LOCUS       DAZUKC010000001        73065 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000001 DAZUKC010000000
VERSION     DAZUKC010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 73065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.001"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            137..508
                     /locus_tag="ACG80T_000001"
     CDS             137..508
                     /locus_tag="ACG80T_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFS4839690.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(782..1474)
                     /locus_tag="ACG80T_000002"
     CDS             complement(782..1474)
                     /locus_tag="ACG80T_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4839691.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(1477..2262)
                     /locus_tag="ACG80T_000003"
     CDS             complement(1477..2262)
                     /locus_tag="ACG80T_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS4839692.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            2499..3899
                     /gene="lacG"
                     /locus_tag="ACG80T_000004"
     CDS             2499..3899
                     /gene="lacG"
                     /locus_tag="ACG80T_000004"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFS4839693.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            3924..4241
                     /locus_tag="ACG80T_000005"
     CDS             3924..4241
                     /locus_tag="ACG80T_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS4839694.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            4254..5927
                     /locus_tag="ACG80T_000006"
     CDS             4254..5927
                     /locus_tag="ACG80T_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFS4839695.1"
                     /translation="MDGIIKQIEKGKAFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            5941..7572
                     /locus_tag="ACG80T_000007"
     CDS             5941..7572
                     /locus_tag="ACG80T_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFS4839696.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(8063..9628)
                     /gene="guaA"
                     /locus_tag="ACG80T_000008"
     CDS             complement(8063..9628)
                     /gene="guaA"
                     /locus_tag="ACG80T_000008"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFS4839697.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(9687..10610)
                     /gene="coaA"
                     /locus_tag="ACG80T_000009"
     CDS             complement(9687..10610)
                     /gene="coaA"
                     /locus_tag="ACG80T_000009"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFS4839698.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            10806..11411
                     /locus_tag="ACG80T_000010"
     CDS             10806..11411
                     /locus_tag="ACG80T_000010"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4839699.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            11719..12735
                     /locus_tag="ACG80T_000011"
     CDS             11719..12735
                     /locus_tag="ACG80T_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4839700.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            12876..14177
                     /locus_tag="ACG80T_000012"
     CDS             12876..14177
                     /locus_tag="ACG80T_000012"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFS4839701.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14223..14885
                     /gene="deoC"
                     /locus_tag="ACG80T_000013"
     CDS             14223..14885
                     /gene="deoC"
                     /locus_tag="ACG80T_000013"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFS4839702.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            14906..15304
                     /locus_tag="ACG80T_000014"
     CDS             14906..15304
                     /locus_tag="ACG80T_000014"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFS4839703.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15370..16452
                     /locus_tag="ACG80T_000015"
     CDS             15370..16452
                     /locus_tag="ACG80T_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4839704.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            16664..18226
                     /locus_tag="ACG80T_000016"
     CDS             16664..18226
                     /locus_tag="ACG80T_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4839705.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18219..19352
                     /locus_tag="ACG80T_000017"
     CDS             18219..19352
                     /locus_tag="ACG80T_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4839706.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19352..20308
                     /locus_tag="ACG80T_000018"
     CDS             19352..20308
                     /locus_tag="ACG80T_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4839707.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20407..21573
                     /gene="deoB"
                     /locus_tag="ACG80T_000019"
     CDS             20407..21573
                     /gene="deoB"
                     /locus_tag="ACG80T_000019"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFS4839708.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            21597..22415
                     /locus_tag="ACG80T_000020"
     CDS             21597..22415
                     /locus_tag="ACG80T_000020"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFS4839709.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22432..23139
                     /gene="deoD"
                     /locus_tag="ACG80T_000021"
     CDS             22432..23139
                     /gene="deoD"
                     /locus_tag="ACG80T_000021"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFS4839710.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(23437..24123)
                     /gene="gpmA"
                     /locus_tag="ACG80T_000022"
     CDS             complement(23437..24123)
                     /gene="gpmA"
                     /locus_tag="ACG80T_000022"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFS4839711.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(24295..24876)
                     /locus_tag="ACG80T_000023"
     CDS             complement(24295..24876)
                     /locus_tag="ACG80T_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4839712.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(25009..25692)
                     /gene="rpiA"
                     /locus_tag="ACG80T_000024"
     CDS             complement(25009..25692)
                     /gene="rpiA"
                     /locus_tag="ACG80T_000024"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFS4839713.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            26011..26424
                     /gene="rpsL"
                     /locus_tag="ACG80T_000025"
     CDS             26011..26424
                     /gene="rpsL"
                     /locus_tag="ACG80T_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFS4839714.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            26508..26978
                     /gene="rpsG"
                     /locus_tag="ACG80T_000026"
     CDS             26508..26978
                     /gene="rpsG"
                     /locus_tag="ACG80T_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFS4839715.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            27072..29159
                     /gene="fusA"
                     /locus_tag="ACG80T_000027"
     CDS             27072..29159
                     /gene="fusA"
                     /locus_tag="ACG80T_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFS4839716.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            29331..30518
                     /gene="tuf"
                     /locus_tag="ACG80T_000028"
     CDS             29331..30518
                     /gene="tuf"
                     /locus_tag="ACG80T_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFS4839717.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            30925..31233
                     /gene="rpsJ"
                     /locus_tag="ACG80T_000029"
     CDS             30925..31233
                     /gene="rpsJ"
                     /locus_tag="ACG80T_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFS4839718.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            31264..31893
                     /gene="rplC"
                     /locus_tag="ACG80T_000030"
     CDS             31264..31893
                     /gene="rplC"
                     /locus_tag="ACG80T_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFS4839719.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            31921..32544
                     /gene="rplD"
                     /locus_tag="ACG80T_000031"
     CDS             31921..32544
                     /gene="rplD"
                     /locus_tag="ACG80T_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFS4839720.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            32544..32834
                     /gene="rplW"
                     /locus_tag="ACG80T_000032"
     CDS             32544..32834
                     /gene="rplW"
                     /locus_tag="ACG80T_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFS4839721.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            32873..33706
                     /gene="rplB"
                     /locus_tag="ACG80T_000033"
     CDS             32873..33706
                     /gene="rplB"
                     /locus_tag="ACG80T_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFS4839722.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            33749..34027
                     /gene="rpsS"
                     /locus_tag="ACG80T_000034"
     CDS             33749..34027
                     /gene="rpsS"
                     /locus_tag="ACG80T_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFS4839723.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            34050..34397
                     /gene="rplV"
                     /locus_tag="ACG80T_000035"
     CDS             34050..34397
                     /gene="rplV"
                     /locus_tag="ACG80T_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFS4839724.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            34411..35067
                     /gene="rpsC"
                     /locus_tag="ACG80T_000036"
     CDS             34411..35067
                     /gene="rpsC"
                     /locus_tag="ACG80T_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFS4839725.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            35070..35504
                     /gene="rplP"
                     /locus_tag="ACG80T_000037"
     CDS             35070..35504
                     /gene="rplP"
                     /locus_tag="ACG80T_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFS4839726.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            35494..35682
                     /gene="rpmC"
                     /locus_tag="ACG80T_000038"
     CDS             35494..35682
                     /gene="rpmC"
                     /locus_tag="ACG80T_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFS4839727.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            35707..35973
                     /gene="rpsQ"
                     /locus_tag="ACG80T_000039"
     CDS             35707..35973
                     /gene="rpsQ"
                     /locus_tag="ACG80T_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFS4839728.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            36031..36399
                     /gene="rplN"
                     /locus_tag="ACG80T_000040"
     CDS             36031..36399
                     /gene="rplN"
                     /locus_tag="ACG80T_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFS4839729.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            36436..36744
                     /gene="rplX"
                     /locus_tag="ACG80T_000041"
     CDS             36436..36744
                     /gene="rplX"
                     /locus_tag="ACG80T_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFS4839730.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            36772..37311
                     /gene="rplE"
                     /locus_tag="ACG80T_000042"
     CDS             36772..37311
                     /gene="rplE"
                     /locus_tag="ACG80T_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFS4839731.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            37330..37515
                     /locus_tag="ACG80T_000043"
     CDS             37330..37515
                     /locus_tag="ACG80T_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFS4839732.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            37551..37949
                     /gene="rpsH"
                     /locus_tag="ACG80T_000044"
     CDS             37551..37949
                     /gene="rpsH"
                     /locus_tag="ACG80T_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFS4839733.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            37981..38517
                     /gene="rplF"
                     /locus_tag="ACG80T_000045"
     CDS             37981..38517
                     /gene="rplF"
                     /locus_tag="ACG80T_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFS4839734.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            38678..39034
                     /gene="rplR"
                     /locus_tag="ACG80T_000046"
     CDS             38678..39034
                     /gene="rplR"
                     /locus_tag="ACG80T_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFS4839735.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            39055..39555
                     /gene="rpsE"
                     /locus_tag="ACG80T_000047"
     CDS             39055..39555
                     /gene="rpsE"
                     /locus_tag="ACG80T_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFS4839736.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            39570..39749
                     /gene="rpmD"
                     /locus_tag="ACG80T_000048"
     CDS             39570..39749
                     /gene="rpmD"
                     /locus_tag="ACG80T_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFS4839737.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            39793..40233
                     /gene="rplO"
                     /locus_tag="ACG80T_000049"
     CDS             39793..40233
                     /gene="rplO"
                     /locus_tag="ACG80T_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFS4839738.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            40233..41528
                     /gene="secY"
                     /locus_tag="ACG80T_000050"
     CDS             40233..41528
                     /gene="secY"
                     /locus_tag="ACG80T_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFS4839739.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            41590..42237
                     /locus_tag="ACG80T_000051"
     CDS             41590..42237
                     /locus_tag="ACG80T_000051"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFS4839740.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            42428..42646
                     /gene="infA"
                     /locus_tag="ACG80T_000052"
     CDS             42428..42646
                     /gene="infA"
                     /locus_tag="ACG80T_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFS4839741.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            42679..42795
                     /gene="rpmJ"
                     /locus_tag="ACG80T_000053"
     CDS             42679..42795
                     /gene="rpmJ"
                     /locus_tag="ACG80T_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFS4839742.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            42813..43178
                     /gene="rpsM"
                     /locus_tag="ACG80T_000054"
     CDS             42813..43178
                     /gene="rpsM"
                     /locus_tag="ACG80T_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFS4839743.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            43206..43595
                     /gene="rpsK"
                     /locus_tag="ACG80T_000055"
     CDS             43206..43595
                     /gene="rpsK"
                     /locus_tag="ACG80T_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFS4839744.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            43675..44613
                     /locus_tag="ACG80T_000056"
     CDS             43675..44613
                     /locus_tag="ACG80T_000056"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFS4839745.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            44641..45021
                     /gene="rplQ"
                     /locus_tag="ACG80T_000057"
     CDS             44641..45021
                     /gene="rplQ"
                     /locus_tag="ACG80T_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFS4839746.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            45293..46132
                     /locus_tag="ACG80T_000058"
     CDS             45293..46132
                     /locus_tag="ACG80T_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS4839747.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            46108..46977
                     /locus_tag="ACG80T_000059"
     CDS             46108..46977
                     /locus_tag="ACG80T_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS4839748.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            46967..47764
                     /locus_tag="ACG80T_000060"
     CDS             46967..47764
                     /locus_tag="ACG80T_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFS4839749.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            47967..48713
                     /gene="truA"
                     /locus_tag="ACG80T_000061"
     CDS             47967..48713
                     /gene="truA"
                     /locus_tag="ACG80T_000061"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFS4839750.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(49168..51837)
                     /locus_tag="ACG80T_000062"
     CDS             complement(49168..51837)
                     /locus_tag="ACG80T_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFS4839751.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(52075..52263)
                     /gene="rpmB"
                     /locus_tag="ACG80T_000063"
     CDS             complement(52075..52263)
                     /gene="rpmB"
                     /locus_tag="ACG80T_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFS4839752.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            52533..52895
                     /locus_tag="ACG80T_000064"
     CDS             52533..52895
                     /locus_tag="ACG80T_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS4839753.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            52947..54629
                     /locus_tag="ACG80T_000065"
     CDS             52947..54629
                     /locus_tag="ACG80T_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFS4839754.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            54746..56782
                     /gene="recG"
                     /locus_tag="ACG80T_000066"
     CDS             54746..56782
                     /gene="recG"
                     /locus_tag="ACG80T_000066"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFS4839755.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            56831..57832
                     /gene="plsX"
                     /locus_tag="ACG80T_000067"
     CDS             56831..57832
                     /gene="plsX"
                     /locus_tag="ACG80T_000067"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFS4839756.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            57954..58196
                     /gene="acpP"
                     /locus_tag="ACG80T_000068"
     CDS             57954..58196
                     /gene="acpP"
                     /locus_tag="ACG80T_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFS4839757.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            58464..59468
                     /locus_tag="ACG80T_000069"
     CDS             58464..59468
                     /locus_tag="ACG80T_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4839758.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            59469..60413
                     /locus_tag="ACG80T_000070"
     CDS             59469..60413
                     /locus_tag="ACG80T_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4839759.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            60413..61375
                     /gene="opp4B"
                     /locus_tag="ACG80T_000071"
     CDS             60413..61375
                     /gene="opp4B"
                     /locus_tag="ACG80T_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFS4839760.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            61402..62316
                     /locus_tag="ACG80T_000072"
     CDS             61402..62316
                     /locus_tag="ACG80T_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4839761.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            62342..64123
                     /locus_tag="ACG80T_000073"
     CDS             62342..64123
                     /locus_tag="ACG80T_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4839762.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            64471..65157
                     /gene="rnc"
                     /locus_tag="ACG80T_000074"
     CDS             64471..65157
                     /gene="rnc"
                     /locus_tag="ACG80T_000074"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFS4839763.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            65177..68758
                     /gene="smc"
                     /locus_tag="ACG80T_000075"
     CDS             65177..68758
                     /gene="smc"
                     /locus_tag="ACG80T_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFS4839764.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            68767..69576
                     /locus_tag="ACG80T_000076"
     CDS             68767..69576
                     /locus_tag="ACG80T_000076"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4839765.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            69587..70585
                     /gene="ftsY"
                     /locus_tag="ACG80T_000077"
     CDS             69587..70585
                     /gene="ftsY"
                     /locus_tag="ACG80T_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFS4839766.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            70772..73039
                     /gene="gshAB"
                     /locus_tag="ACG80T_000078"
     CDS             70772..73039
                     /gene="gshAB"
                     /locus_tag="ACG80T_000078"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFS4839767.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
BASE COUNT        23652 a        13285 c        15981 g        20147 t
ORIGIN      
        1 cactcagaga acgggttggt ttttaataga tttaataagt actgataatg aaatttctcc
       61 tggttgggaa aacgaagttg gttaccctgt agaagaattt tttcaagatt actttgcgga
      121 gtatgccgat aggataatga gagattacgt aacagataaa actcaagaag tcatcaatca
      181 atcccagtca ttaaatgaat tatctgataa ggcgaaagaa gaagcagttg ccattatttc
      241 ttcagggatt agtgactata ttgatatgag tggtgaaatc aattatggcg ataaagacaa
      301 aattgtacgc atgataaaag cttttatcaa aacttttgat gtgaaggcag atcttaagtt
      361 taatgatact tacctagtag gaaaattaat taatatcctt gggacagaga aagatacgga
      421 aacgtttatt tcctctttgt ctatgttgtt tacagataag catttttcaa ctcagcttgg
      481 tggagaggaa ctagtggaaa tcttttaacc attacgccca gcattaataa aagtatatca
      541 aaaaagtact cctgatgatt tttaagattg gtttgagaaa taatgtgaaa acactatttt
      601 taatgttaat tatagataga gatgaacgcg tcatatggtt aaaagaatga aaaagtcaaa
      661 gatacactgc tatatttcac taaggatata gcagtgtatc tttcatgtcg tttcactggc
      721 agttttttat aaaaggaggt gtgcatttca gttgcttttc tttggtagtt agttgaatcg
      781 tttaagaatt aacggatgtg gcagacaggt gctctgtcca aaaaccagta aaatctttga
      841 tggttccatc tttatttagc tgtgcctgtt caaatactgt tttgttttta ccgaatggat
      901 caattgcaat aattgttccg atccccgcac gtttggcagc aagtaatcct gagtaagcat
      961 cttcgataac aagacaatca gccggagcta agctcagttt gtctgccgct ttaagaaaaa
     1021 tatccggttg cggtttccca gggaaagagc cgtcgtcata gacaatcttg tcccagtcaa
     1081 accagcgggc taaatcgaaa atatcaaaat aaaaagcaac attctctttg acagtagccg
     1141 tggcaatcgt taatggaatc tgttggtcag tcagctggtc aagagtcgct tcaagccctt
     1201 ttgtcaactg aagctcgttt tcattttgca agcaaagcat acgataatag ctttcttttt
     1261 cttctgacaa tacggcgatc tcagaggcgg tcaaatgctc tgaaatgaaa tgtttcaaaa
     1321 tctccgtatt cgtccgacca tgaatatttt ttaaaatatc ctcgtctgat aaaccatctt
     1381 ttgtatgatc atgtatcata tgcagccaag cagcttcatg cagatgagag tccaaaaaca
     1441 ttgttccgtt gaaatcaaat ataatccctt tcatagttac gctcctatag tggtattttc
     1501 ggttgaatca gcaatttgca gatcgacata ttgtgcatag tgctcttgaa cctcccgcga
     1561 gatctggcga tccgtaatga gcagatcgaa atcatgcaaa ttataaaaca cataaaaatc
     1621 atctcgattg aattttttat tatcaacgag tagatatttc ttacgggaat tatttagagc
     1681 gatctgctgt gcttctcctt cttcggtact gtaagtagag atcgcttcgt tgtgaacgcc
     1741 atttgaactg ataaatgctt ttgtaaactt tagtcttctt aaattgtcgt tggtgatcgg
     1801 tccgacaaat gcgccggtat ttttccggaa atcaccgcca atcagtagaa tttctggtcc
     1861 atctgcttga gcaagaatat caaaaactgg ataattattt gtaatgatcc gaattttctt
     1921 gttcagtaaa tgcgtcgcca atagttcgat tgttgtacct ggtcctaaga aaatcgtttc
     1981 tccctcgtga atcaaggagg ctgctgtggc agcaatttct tttttttctt gaatctgcag
     2041 agtggctttt tcagtgtgag aaagttcttg atctaaagtg taggaaagac tttgagcccc
     2101 tccatggaca cgcagcagct tgcccgattt ttctaattca tctaaatctc tgcgaacggt
     2161 catatcagaa acatctaatt gattgatgat ttcattaacg gtaaggattc cttttttatt
     2221 gaccatttct acgatagtaa gcaatctttc ttttttcaac attgtcttca cctctaattt
     2281 aaggataatc tatcatataa acggacaaca gtcaatctaa ttcgaacaaa agtaaacatt
     2341 tagggtggtt tttatgtttg ttacaataga ttttattgct gttttctggt ttttatgatt
     2401 atttgtgttt gtttttgttg acaaatgttt tggatgcgtt tacaatgcaa tcagttagaa
     2461 agagcgaaaa ctgattatag atttgaggag agatgtacat gcttaaatta ccagaagatt
     2521 ttatttttgg cggagccaca gcagcctatc aggttgaagg agccactaag gaaggtggca
     2581 aaggcgctgt tgcttgggat gactttttag aggaacaagg gcgattcagt cccgatccgg
     2641 caagtgattt ttatcatcaa tatgcaaagg acattgaatt gtgtgaacgt tttggtgtaa
     2701 atggtctaag attatccatt gcttggagcc gcatttttcc tgatggagct gggaaaccaa
     2761 atccagaagg aatcgctttt tatcatcgtg tgttcgaaga atgtaagaaa agaaacgtaa
     2821 cgccgtttgt gacgttgcat cattttgata cccctaaacg tttgtttgac catggagatt
     2881 ttttgaatcg agaaacaata gaagcatttg tttcctatgc cattttttgt tttcatgaat
     2941 tcaaagaagt caaggtgtgg agtaccttta atgaaatcta tccagttgca accaatcaat
     3001 atttgttagg tgttttccca ccaggaatca aatatgattt tacaaagatc gtcgcctgtc
     3061 ttcataatat gatggttgct catgcccgtg tagtgaacta tttcaaagaa aacgaacttc
     3121 cgggtgaaat tggtgtagtt cactcattag agacgaaata tgcggcaaca gacgcgccgg
     3181 aagataagca tgctgccttt ttagacgatg cattgtcgat ccgtttctta ttagatgcta
     3241 cctatttagg ctattattca actgaaacct tgactgctct tgatgagata tgtgaagcaa
     3301 atcaggccag ctatcatttt cctgaagaag atttcgttga attgaaaaaa gcatccacgc
     3361 gtaatgacta cttagggatc aatcactacc aatgccattt tgtcaaagct tacgatggag
     3421 aaaatgcaat ccatcataac ggtacgggtg aaaaagggac ctctgtttat aaagtgaagg
     3481 gaatcggaga acgtatttat aaagaaggaa ttcctcgaac tgattgggac tggctgattt
     3541 atccagaagg tctatatgac ttgttgctgc gaattaaaag cgattaccca cattacaaca
     3601 aaatctatat cactgagaac gggatggggt ataaagatca gtttgaagat ggcattatta
     3661 tggatcagcc gagaatcgac tatttgagag tctatttgga atccttaagc aaggcgataa
     3721 cagccggggt aaatgtgaag gggtattttt tgtggtcgct tatggatctt ttctcatgga
     3781 caaatgggta taacaaaagg tatggcttgt tttatgttga ttttgaaacc cagaagcgtt
     3841 acccaaaaga atcagcttac tggtacaagt tggtcagtga aaccaaaacg attatttaat
     3901 aagtgataat ggaggaagaa aagatgaata aagaggaatt acaaatgctt ggttttgaga
     3961 tcgttgcata ttcaggcgac gctcgcagta cattattgaa attattgaaa gaagtaagac
     4021 aaggaaactt tgaacatgtt gattcggcgt tgaaagaagc cgacgaaaat ttaacccttg
     4081 cacataattc gcaaacaaaa attttagcgg aagaagcatc tgggaaagag atggagatgg
     4141 gattcatttt tatccatggg caggatcatt taatgaccac tttattgctg agagatctga
     4201 ttcaagactt tattgtactg tacagacaga atcaaggata ggagaagaac gctatggatg
     4261 gaatcattaa acaaatcgaa aaagggaaag cattttttga aaaaatctca cgtaatagtt
     4321 atttaggagc cgttcgtgac ggatttttaa cagcgatgcc agctatttta ttttcaagca
     4381 ttttcatttt gattgctgcg gttcctgaga tttttggtta ccaatggcct gaatctgtct
     4441 ccacatggct atggaaagtc tataattatt cgatgggtgt tgtagggtta ctcgtttcag
     4501 caaccactgc tcgctgcttg gctgattcga tgaatcgtcg cttaccgaaa aacaaagtaa
     4561 tcaacaccac ctctgtgatg ctggcctctg tggtaagttt cctgttgtta agcgtctctc
     4621 cgattgaagg tggattttca acggaatata tggggacaaa gggactactg gcttcctttg
     4681 tcgctgcatt tttaacagtt aatttatata agttttgtgt gctgaaagat attaccatta
     4741 aaatgcctaa agaagttcct ggaacgattt cgcaaacctt ccgagatatt tttcctttct
     4801 ctttttcagt atttgcggcc gttttaattg atttaggcat tcgacaaatc ttccaaatct
     4861 catttgcaga agcaatcatt acggtgctgc agccgctatt tacagcagct gacgggtact
     4921 taggaatcgc tattatctgg ggagcgatgg cgctgttttg gtttgtcggt gttcatggac
     4981 cgtcgatcgt ggagccggca attgcggcaa ttatctatgc caatgtggaa accaatctgc
     5041 aattgttcaa ttccggtgaa caagcttcta aagtactgac ggtgggttta gggaattttg
     5101 taggaactat gggaggaact ggggcaacat tggttgttcc ttttatcttc ctactctttg
     5161 ctaagtctaa gcagttgaag gcagttggga aagcttcctt tattccagta agtttcgctg
     5221 tcaacgaacc attgctattt gcggcaccca tgattttgaa cccttatttt tttattccat
     5281 ttttgttaac acctgttgta aacgtttgcc tgttcaagtt ttttgtcgat gtattgggca
     5341 tgaacagctt tatgtacgtt ttgccttggg caactccagc accgatcggt ttggttctag
     5401 gaacgggcat gggtatctta gcttttgttc tagcagcggt tctaatcgtc gttgacttcc
     5461 tgatctatct accattttgc aaagcatacg atgctgtctt agtagagaaa gaagcacaaa
     5521 aagcagaggt gatcgcaacc gaggaaggtg cttctgccga tgtacgtgag gaagtacctg
     5581 tggcagcgag tcaagttatc agcgatgcaa ataaagaaat caatgtgttg gtactttgtg
     5641 ctggcgcagg gacaagtgcg atgttagcta atgctttaaa aaaaggtgca tctgaatcga
     5701 aagcaccgat cagtgcaatg gcgggtgctt atggttctca ttacgaaatg ctgcaggatt
     5761 ttgatatggt ggtcttagca ccgcaagtca attcttacta tgatgatatc aaagaagaca
     5821 ccgatcgctt aggaatcaag ctggttgcga caaaaggagc agaatatatc ggattaacga
     5881 gagatcctga aaaagctgtt cagtttattc tgaaccaatt tcaataaagg aggagccata
     5941 atgaaaatgg gaatttggtt gcgtaaagct gttttatttg cagctgtgtt aggggctttg
     6001 ggggctgttt caaaaactgt tgaagcatct gccagcgaat ttattcgcgg agcagatatt
     6061 tcgatcctgt cagatatgga aaaatctggt gcaaaatatt atgaagacga tatttcaaaa
     6121 gatgccttag agattttaaa aaataatggt gtcaactatg ttcgactgcg tttgtggcag
     6181 gatccttata atagtaatgg agaaagttat ggagcaggga caaatgatct ggaaacgacg
     6241 attgaccttg caaaacgagc aaaaaatctg ggacttaagg tgctgctgga ttttcactac
     6301 agtgattttt gggttgatcc tgggaaacag aatcttccta aagcatggca aggtctgacc
     6361 tttgaagaaa tgaatacggc attgtacgac tatacaaaga acgttctgtc ggaaatgaag
     6421 cagctggacg tttatcctga tatggttcag atcggcaatg aactgaatag cggaatgctc
     6481 tggccttatg gcaagtcttg gggagaaggc gggggagaat ttgatcgatt ggctgctttt
     6541 ttgaagtctg gtatccaagc tgttcgagac actcaaccta aaacaactcc agtaatgctc
     6601 cacttagctg atggtggcga tacaggagca tttacttggt ggtttgatga aatcacgagt
     6661 cgaggtgtgt catttgattt aattggagtg tcgtattatc cgtattggca cggttcaatg
     6721 aatgaacttc aaaccaatat gaatgcgatc agccaacggt atggtaaaga agtgatcgta
     6781 gttgaaacag cttatgggca tacaacagcg aatgccgata ctatgccaaa tgcttttggt
     6841 gaagccgaag cagctgcggg gggatatgaa ccaacaccga caggacaagc agagtatttg
     6901 ctggatcttg ccgatcggat tcaggcagta ccgaataatc gcggggcggg cttcttctat
     6961 tgggaacctc tttggtacaa cggaaatgtg agttgggcaa cacaagcagg tatgaattat
     7021 ctcagtgttc aatccactat gggaaacgaa tgggacaacc aagcgatgtt tgattttgct
     7081 ggaaacgcct tgccaagctt gcgtgcgttt aaacaagcag gggcgcaaat aaatctagtt
     7141 aaaaatgcaa gtttcgaaag cgatggacca acgaccagtc cttccaattg gcaaacctgg
     7201 tttcaacaag gaaccgctaa gggtacgatc aaaacggaag cgggcacttt ttttggggac
     7261 tacaaactaa ctttttggca ggatcaagca tacgaggcat cagcttatca aaatgttagt
     7321 ggcataagca aaggaactta cacattgagt gcctatgtaa tgtctggggg agagtttgat
     7381 actaatcagt tatatatcaa aaattatggt ggagcggaac taaatactgc ggttgttaaa
     7441 acaagtgaat ggacaaaaat tacaatcaaa gacattccaa ttacaacagg tgtttgtgaa
     7501 attggggttt atagcaaggc ttcaggaaat gcttggacga acgttgattt tgtttcattg
     7561 gtgaaacaat agactgcgga tcgtttcgat tcaagatcgg aggttccctt cagccacatc
     7621 gttagcgtag aaggcaggaa tcgaacctgc acctcgcctt tgccaaaaat aaccagaaac
     7681 gatttccagt catttttacc aaaacgagta aagcaccaga cttcatctac actttgtatt
     7741 atgctaaaga gagttcaacg atttcgtaag aagcaatgaa aaaattgcaa aaagttgtgc
     7801 agataacata aagtttgttt aagaaagggt aaccttagaa ttccgtcgtg aatatgattt
     7861 gagcacttag atggatagaa aaaagttctc ggaaaacagt tcgtttttcg agaacttctt
     7921 tttatgataa gaatgaaaca aattaactta tattatagtc tcttttggta gggaaccatc
     7981 ttatttttaa tgaaaatcaa gcattttagc tattgaagaa tgttgattta tagggattcc
     8041 ccctccatat tatgttggct ctttattccc actccactgt tgctggtggt ttagaagtga
     8101 tgtcatacac gatacggttg acgtgggcta cttcgtttac gatacgtaca gagatttttt
     8161 gcaagacttc ccatgggata cgggcaaagt cagcggtcat tccgtcgatt gaagttacag
     8221 cacggatacc gactgtgtag tcgtaagtac gtccgtcacc cataacaccg acagagcgga
     8281 tccctggaag aacagtgaaa tattgccaga tatcacggtc aagtccagca gctgcgattt
     8341 cttcacgcaa gatagcatca gattcgcgta cgatttctag cttgtcttca gtgatttcgc
     8401 caagaacacg aataccaaga ccaggaccag ggaatggctg acgccaaacg attgaatcag
     8461 gcatgcctaa ctgagtccct aattcacgga cttcgtcttt gaataatgtg tttaatggtt
     8521 cgatcaattc aaattgcata tcttctggca gtccacctac attatggtga gatttgatcg
     8581 tctgtgctgt ttctgtacca gattcgataa cgtcagtata caacgttcct tgtgctagga
     8641 aagagacgcc ttcttcacca gcaagttttg ttgcttcgtc atcaaaaaca tatacgaatt
     8701 cattcccaat gattttacgt ttttgttctg ggtcagatac acctttcaac ttgtctaaga
     8761 agcgatctct tgcattgact ttgatgatgt tcaagccgaa ttttccgcct agactttcca
     8821 ttacttgttc cgcttcgcct ttgcgtagca gaccgtggtc aacaaagata gatgtcaatt
     8881 ggtcaccgat cgctttttgt aaaagaaccc caacgacact agaatcaacg ccacctgaaa
     8941 gaccaagcaa gacttttttg tcaccaactt gttcacggat tttttctact tgcatatcga
     9001 taaagttatc cattgtccag ttaccttcac agtgacaaac atcaaaggca aagtggcgca
     9061 gcaattcgat tccgtattcg gaatgacgta cttctgcatg gaattggata ccataaaaat
     9121 tacgtgttgt gtcttggata gaagcaatcg gacaatcttt gcttgttccg actgtttcaa
     9181 agccttcagg aactttagtt accaagtcac catggctcat ccatactgtt tgttgttttg
     9241 gtgtttcttt aaacaagaca gcttgatcgc ttgttacttc taacaaggct ttcccatatt
     9301 cgcggttttt agcaggctct acggaaccgc caagtttgta cgtgatcaat tgcataccat
     9361 agcaaatccc taatacagga atacctaaat tgaagatctc aggatcgatc ccaaatgcat
     9421 tctcatcata tacactgttt ggtccgccag aaagaatgat tccctttgga ttcatcgctt
     9481 ttacttcttc tgcagtaatg cggtggctca ataattcgga aaagactcca agatcacgaa
     9541 tacgtcttgt gatcaattgg ttatactgac taccgtagtc taatacgata atcttttcaa
     9601 cgttcgtcat atcggcaacg tttgtcaaat ttattacccc tatcccttta aattttatct
     9661 caaaatagtc aaactatttt aaaatgctaa tattttcgca tgaaaatcga atcgatgata
     9721 tgattttcag ttttatggag aattatatcg gctcttcctc gtgtaggcaa gatatattcc
     9781 tgtaaatttt tcaaattcac atttttccat acatcttttg ccattttaaa ggcttcttca
     9841 cgatctccga tagcaaaacg ataataataa ttctctggat tttggaaagc cgtatccagt
     9901 aacgcaccaa aacgatccaa ataccatttt tcgatcagca cgggatcggc atcgacatag
     9961 acagaaaaat caaaaaagtc gctgacgtag atttgctgat tcgcaggtaa ttgaagcgta
    10021 ttgatcccct caacgatcaa gatatcaggc tgctggatca attcgtactt gtctggaatg
    10081 atatcataca catcatgtga gtaaacaggt acttttatct cttcctgacc agattttaca
    10141 cggttcaaga aatcgatcag catctccata tcataacttt caggaaatcc tttacggtcc
    10201 atgattccac gctcttttaa tatacgattt ggataaagaa atccgtcagt cgtgatcagc
    10261 tgaacgtttc ttcgtttgaa ggtacgagac aaaatcgtct gcagcagtct ggctgttgta
    10321 ctttttccaa cggctacact tccggcgatc ccgataataa aaggcggtac ttctacgtat
    10381 ttatgaagaa ataaaccttt actcaagaac aaagattcaa attctttcat ataaagataa
    10441 atcagatgag caagaggtac atagatatct cgaacatcct gcatggaaat gcggtcattc
    10501 aaacttttga tattatctag ttcatcttgt gtcaaaggtg cttgcccatt acgataaaat
    10561 tctcgccatt cttctcttgc cacacggtag tagttcattg gatcattcat attagcctcc
    10621 tgcgttctcc catcaatgga actattttaa cacaaaataa acgcaaaccc caccacttaa
    10681 gagagattgc cgatcttttt gtataatctt tataatagaa agaaacggag tcttattctt
    10741 gacttgcgca cggatacata aaaggataga ctgttgtcat gaaagaaatg agaaggatgg
    10801 aagacatgac aaatcattac tattctgaac gcccagatac ggcgcacgat tttgaacaat
    10861 gggcttttga gttaaaaggc agaacctttc aatttgtaac agattctggt gtattttcac
    10921 gtgaaacagt ggattatggt tcacgtgtcc tgattgatgc tttcgattgg gaagagttac
    10981 ctgatgaagg aaagatctta gatgtgggat gcgggtatgg accgattggg ttagctttgg
    11041 catttgcgac acagcggttt gttgaaatgg tcgacattaa tgaacgagcg gtaagtctag
    11101 cgcaaggaaa cgcaaagcgt aaccaaatcg aacatgttga tatccatcaa tcgaatatct
    11161 atgaagcagt tcatgaagaa acctatgcag ctatcgtaag caatccgccg atccgtgctg
    11221 gaaaaaaagt tgtacatgaa attttgacag gcgcatatcc tcggttgaag aagggcggta
    11281 cactgacaat tgtgatccag aagaaacaag gagcgccgag tgcgcaaaag aaaatggaag
    11341 aaacatttgg taatgcagaa atcgtcacaa aagataaagg atattatatc atcaaaagtg
    11401 tgaaagaata actagctgaa aaatagtgga gcgacgaaag agtggaactg ggcgtgcatg
    11461 tacgggtacg tatcagttct ttttttgttg agaagttttt aaaaacaaag aactattttt
    11521 tcttcaaaaa gaaatagtag gaacagagtc gttgtttttt acgataaaat aaataagata
    11581 cagaacttgt atagaaggct gccaagtcgg tagataaagg aggtttttat caaaaaacat
    11641 actttccttt tttctctatt cttttcttgg gggttttggt acactgatag ggaatttaga
    11701 tagaagggag ccaataggat gaagctgacc atcagagata tcgcagaaat ggcaggcgta
    11761 tcagtaacaa ctgtctcaca gattttgaat aacaaaggaa gcagatttag tgataagaca
    11821 agaaaaaaag tgttgtcgat cgtgaatgaa catcattata agcccgatta tttcgcttca
    11881 aatgtgatca atcgacattc aaagaccatc ggaatgatcg tccctgatgt cactgatttc
    11941 ttcttctcca aactgatcga aggtgtagag agctatttga atcctttggg ctatgtgatc
    12001 atgttatgca attctcgtca tagccaagaa aatgagatca agtatttaca agagctttcc
    12061 caccgctcag tggacggaat cttgctggcc acaccaaata ttttacctga gaaatatgca
    12121 ttagacagcg gtttttatca aaaaatgcca atcattttaa tcgatcgcgg attgaatcgt
    12181 cgggataacg gacggcttat cgtaaaggaa tatgagggcg cctatcaagc tgtttcgctg
    12241 tttattgaaa atggccatac gaagatcggc atgctgaagg aaaccactgg atactatcaa
    12301 ttagaagaac gtttcaacgg atatcgtcat gcgctgaaag atcatggcct gccttttaat
    12361 gggaaatttg ttgaacatgg agagttgact gtccaaggtg gatatactgc atcaaaaaaa
    12421 ttactcaaac ataaagatat cacggctatt ttttgcggga atgatgccat ggcgatcgga
    12481 tgctatcagg cgatcgatga attgggcaaa aaaatcccag aagatatctc agtcatagga
    12541 ttcgatggat taaagctgtc agaatacatg attccgcaat tgacgacagt gcagcagcca
    12601 agttttgata ttggatttta tgctgcacgt ttcctgatcg atacgatcga atttcctcaa
    12661 agaaaagttc cgaacaaagt gttcgaaaca aaattaatta ttcgtgagag cgtaaaagta
    12721 ttgacaaagg gctaactcgg cggtatattg cttatgtaag catttacaaa ggctagttag
    12781 tgcttttttt atttttagta aaccagttta ctaaagtggt ttacctttcc taaaaataat
    12841 gtataatacc gaatgaagac aaggaggatc aggcaatgcg aatggtggat ctgattgaaa
    12901 aaaagaggga tggaaacgag ctgtcgaaag aggaaatcga gtacatcgtc acgaactata
    12961 cgaatggaaa gattccagat tatcaagtca gtgcattatt gatggcaatt ttttatcaag
    13021 acatgacaaa tgaagaaatc acgaacttaa cattggcgat tgctaattct ggtgatgtca
    13081 tcgatttgag ttctcttgaa ggaataaagg tagataagca ttcgacaggt ggcgttggtg
    13141 acacgaccac attgatttta gcccccctag tagctagtgt cggagtgaca gtggctaaaa
    13201 tgtctggacg cggtctaggt tacacaggtg gtacactaga caagctagaa gcaattcccg
    13261 gatttcagat cgagctttcg gatgaagaat ttgtacgcat tgttaatgaa agtaaagtag
    13321 cggtaattgg tcaatcagga aacttggcac cggcggacaa aaaattgtat gccttgcgtg
    13381 acgtcacagc gaccgttgat tctcttccgc tgattgccag ttcgattatg agtaaaaaaa
    13441 tcgcggcagg agcagatgca atcgttttgg atgtgaccac tggagacggt gctttcatga
    13501 agaacatcga agatgcaaga cgtctggcaa agaccatgac gagcattggg aaactggcta
    13561 accgagaaac agtggcggtt atctcagata tgtcagaacc tcttggcgaa gctatcggaa
    13621 atagtctaga agttgtcgag gcaatcgaaa cattacaagg aaatggtcca gaagatctag
    13681 tagaaatgtg ctacgcctta ggaagccaaa tggttgttct tgctggcaaa gctaagacaa
    13741 ccgatgaagc aagaacgttg cttcaagaag ctctggaatc aggtaaagcg ttagcaaaat
    13801 tcaaagaaat gatccaaaat caagggggag acccagcgat cgtggaacat ccagaacgaa
    13861 ttttgactgc acgctacaca atggagcttc ctgctaaaca aagcggagtt gtgtcaaaaa
    13921 tcgtggcaaa tgaattagga attgctgcaa tgatgctagg tgctgggcgt aaaaccaaag
    13981 aagaagatat tgatcacgct gttggattga aactgcataa aaaaattggt gacactgtga
    14041 caaaaggtga gtcactattg acgatctata gtaacgacga agaaatcact tctgtcattg
    14101 aattattata taaaaatatt gaaattggtg aatcggcaat gaagccaacg ttgattcatg
    14161 acatcatcac agaataaagg aaaaaagcgg actttaccgt tttaaagaaa aggggaaaag
    14221 aaatggaact aaaccgaatg atcgaccata ctattttgaa agcagacgca tctaaagaag
    14281 ctgtgatgca aattatcgaa gaagcaaaaa aataccattt ttattctgtc tgcatcaatc
    14341 cagcatgggt tgcgctagcg aaagaacaat tacaaggtga gccagtagcc gtatgtacag
    14401 taatcgggtt tcctctagga gcaaacacac ccgagacaaa agcatttgaa acaacagatg
    14461 ccatcaataa cggtgcagat gaagtcgata tggtcatcaa tatcggtgct ttgaaatcaa
    14521 aggatgatca acaagtccaa aaagatatcg aagcagttgt tgaagcagca aaagatcgtg
    14581 cgcttgtcaa agttattatt gaaacatcgt tattagatag agaagaaatc atccgagctt
    14641 gcgaaatagc aaaagctgca ggcgctgatt ttgtcaaaac atcaactggt ttctcaacag
    14701 gcggagcaaa ggtagaggat gtccgtttga tgcgtgaaac tgtaggacca gaaatgggtg
    14761 tcaaagcttc cggcggtatc cataacgaag aagaagcaat ggcaatgatc gaagctggtg
    14821 ctacaagaat cggaacaagc gcaggagtag caatcgtatc tggttcaact ggtgaaggct
    14881 attaattttt tcaaaggaga agctcatggc agaactcaaa caagaatgga tcaacacagc
    14941 tattgaagcg ttagataaag cctatgttcc ttattcccat tttccggtag gcgcttgtct
    15001 cgtaacggaa tcaggcaaga tttatcaagg catcaacatt gagaatgcat catttggatt
    15061 aacgaattgt gccgagcgca cagctttttt caaagcagta tctgaaggag aaaaaacatt
    15121 tacgcatcta gtcgtagcgg ggcacacccc cgatcctatc tctccttgtg gtgcatgtcg
    15181 acaagtaatg gcagaattct gttcccctga tatgccggtt acgctagtag gagacaatgg
    15241 tgtgacgaag gcaacgaccg ttcgtgcact gctgccttat gcatttactg aaaaagatct
    15301 ataactcatg ttcattcagt cagctgactg ttgaaaaata aaacaattat tttaggaggc
    15361 tttacagaaa tgaaaaaagc aaaattattt ggtttaggtg ctgtcgcact agcagcaggg
    15421 ttatttttag gtgcatgcgg caacaacggc tctactgatt catccggtgg caaaagctca
    15481 tcagacacaa caactgcagc attgatcaca gacacaggtg gtgtagacga ccgttcattc
    15541 aaccagtcag cttgggaagg actagaaaaa tgggggaaag atcaaggact ttcacgagga
    15601 aatgatggct tccaatattt ccaatcttca aatgaatcag attacatccc aaatattgac
    15661 caagcattga atgctggatt caaaacgatt tttggtattg gttataaact aaaaccagcg
    15721 atccaagaac aagcaacaaa caatacaggt acgaactttg ttattatcga tgatgtcatc
    15781 gatggattag ataacgttgt atctgcaact ttcaaagaca acgaagcttc ttatcttgca
    15841 ggtgttgcag cagcttatac gactgaaaca aacgtagtcg gctttatcgg cggtgtaaaa
    15901 ggtgaagtaa tcgatcgttt tgatgctgga ttcaaagccg gtgttgatgc aggtgcgaaa
    15961 gaattaggaa aagaaatcaa agtattgaat caatatgctg gtgacttctc tgctccagat
    16021 aaaggacgtt caattgctca aggtatgtat gcacaaaacg cagatatcat cttccatgct
    16081 tcaggcggta caggtaacgg tgtattccaa gaagcaaaat cattgaacga atcaggcgac
    16141 aaaaaagttt gggttatcgg tgttgaccgc gaccaatcag acgaaggcga atacacatta
    16201 aacggcgaaa agaaaaactt cacattgaca tctacactga aagcagttgg aacagtagta
    16261 gaagatttgg cacaaaaatc agcggacggt aaattccctg gcggcgaaca cactgtttat
    16321 ggattgaaag aagacggtgt tggtctaaca gaaggacaac tttctgacga agcgaaaaaa
    16381 gcagttgatg aagcaaaaga aaaaatcatt tcaggtgacg taaaagttcc agaaactcct
    16441 gaagaaaact aaaaccattt ctatagcctt gttagacaca tgtttgacaa ggctatggtt
    16501 tgattagaac aagaattgtt catttatcca agctgccttg tgacagtttg gataagggaa
    16561 caactccctg aagtaatgtt tgtttcaaaa gaagaacgct cactaagcgt gaatcttagt
    16621 caatcgttgc cctagataga aatcgttagg atgtgaaaaa aaggtggttc aagaaaatat
    16681 tgtcatagaa atgcagaata ttaccaaaga atttggaaca ttcaaggcaa atgacaacat
    16741 caatttacaa gtaaaggcag gagaaattca tgcgttgctt ggagaaaacg gtgctggcaa
    16801 atctacattg atgaacgtgc tttccggatt attagagccg acatcaggga aaattttgat
    16861 gcgtgggaaa gaagtaaaga tcacaagtcc gaccaaagcc aaccaattag ggattgggat
    16921 ggtccatcag cactttatgc ttgttgatgc ctttactgta acagaaaaca tcgtgttggg
    16981 aagcgagcct agtcgcgcag ggatgcttga ccataaaaaa gcacgaaaag agatccaaaa
    17041 agtttctgaa cagtatggat tgtcagtcaa cccagatgct tacgttcgtg atatttcagt
    17101 tggaatggaa caacgggtag aaattttaaa aacactttac cgaggagcag atgtactgat
    17161 ttttgatgag ccgacagctg tattgacccc tcaagaaatt gatgaattaa tcgtgatcat
    17221 gaaggaatta gtcaaagaag gcaagtcgat cattttgatt acgcataagt tagatgaaat
    17281 caaagcagta gctgaccgct gtacagttat ccgccgtgga aaaggaatcg gtacagtcaa
    17341 cgttaaagac gtaacctcac aacaattagc cgatatgatg gttggaagaa cggtttcatt
    17401 caaaacgatg aaaaaagaag cgaagcctca agaagtcgtt ttgtctattg aaaatctagt
    17461 ggtgaaagaa aatcgtggat tagaagccgt gaaaaacttg aacttagagg ttcgtgctgg
    17521 cgaagtactt ggtatcgctg gaatcgatgg aaacgggcag tcggagttga tccaagcttt
    17581 gactggtttg cgaaaggcag aaagcggaca tatcaagcta aaaggggaag acatcactaa
    17641 taaaaaaccc cgaaaaatca ctgaacatgg tgtaggacac gtgccagaag accgtcataa
    17701 atacgggtta gtcctagata tgacattatc tgaaaatatc gcactacaaa cctatcatca
    17761 aaaaccttac agtaaaaacg gcatgctgaa ttattcagta atgaatgaac atgccagaga
    17821 attgatcgaa gaatatgatg ttcgaacaac gaatgaactt gttcccgcaa aagctttatc
    17881 aggcggaaat cagcaaaaag caattatcgc tcgtgaagtc gaccgagatc ctgatctgtt
    17941 gattgttgca aatccaactc gtggactgga tgtaggtgcg atcgaattta ttcataaacg
    18001 tctgatcgaa caaagggaca aatacaaagc agtgttactg atcagtttcg aattagaaga
    18061 aattttaaat gtttcggatc gtattgctgt tatccatgaa ggagaaattg tcggtatcgt
    18121 tgatccgaaa gaaacatctg aaaatgaatt aggtctattg atggcgggct ataccttaga
    18181 agaagcacga aaagagttag aaaagggggc tggcgcacat gaatgatcgc agcgaaaaat
    18241 tccgtaattt attagtgcct gtactttcag ttgttttagg attgatctta ggcgcgatat
    18301 tgatggctgc ttttggtttt cagcccatcc aaggttattc ttctatgcta gatgcagccc
    18361 ttggttcgca aagagggata ggtgaaacgt taaggcaagc aacgccgttg atttttacag
    18421 cattaggttt ttctgtagct aactcagctg ggtttttcaa tattgggctt tctggacaag
    18481 cactttgcgg gtggatcgtc agtgtctggg tggcacttgc gtttccagat ctgccaaaag
    18541 ttgttttgct tccgttatgc gtgatcttag gtgccttagc tggtgcgatg gcagcggctg
    18601 ttccaggttt acttcgagca ttttttggaa caagtgaagt gatcgtaaca atcatgatga
    18661 actatatctt attatacgta agcacatttg ctttacatga agtgatgcct gaaagctatc
    18721 gctcaagttt ggactcttcc aatatgatca gtcaaaatgc ctcactgcgg ttaccatggt
    18781 taacacaagc ttttggcggc tctcgcgtaa actcaggatt atttttagca atcatcgcgc
    18841 ttattttcgt ttggctgctg atgaagaaaa cgactttagg atttgaaatc cgttctgttg
    18901 gattgaatcc ttttgcttct gagtatgcag gaatgagtag taaacgtacg atcgttttgt
    18961 cgatggtcat ttctggcgct ttggcagggc ttggcggtgt ggttgaagga ttaggaacgt
    19021 atcaaaactt ctttgttcaa acgacttcgc tatctattgg atttgacgga atggctgttt
    19081 ccttgcttgg ttctggatca gcaatcggta ttcttttatc agcgatcttg ttcagtattt
    19141 tgaagatcgg tggacttggc atgcagacag gagcaggtgt tccgtttgag atcgtcaatg
    19201 tatcgattgc tttgattatc ttctttgtcg ggatcaactt cctgattcgt ttcgtcatgg
    19261 ctaaagtctt ccaagggaaa aaacaagaag agatcgttgc gacaatagaa tcgaaaccaa
    19321 cgaatcaaaa ccatgaaggg ggcgaactat aatgtcgact atcgaattaa ttgccttgat
    19381 ccttacttct acactggttt attcaacacc gctaattcta acgtcactag gcggaacttt
    19441 ttctgaacga agcgggatcg taaacgtagg gctagaggga atcatggtca tgggtgcttt
    19501 tagttccatc gttttcaact tgacgatgag tccatcactt ggttctatga caccatggat
    19561 cgctatttta gtgggtggcg tcgtaggtat cctattttct ctgattcacg cagtagcaac
    19621 agtcagtttg cgtgctgacc atattatcag cgggacagta attaatctga tggcacctgc
    19681 tttaggtgtt ttcttgatca aagcatggta tggtaaaggt caaactgata acatcacgca
    19741 gaatctgggg tatttttctt tcccaggatt agcagatatt ccggtcattg gaccaatttt
    19801 ctttaaaaat acattcttac ctgcttattg tgctattttg attgctgtcc tagcttgggt
    19861 gatcatcttc aagacgaaat tcggcttgcg cctccgttca gttggtgaaa atcctcaagc
    19921 tgccgacaca ttaggtatca atgtatatgc tatgcgttat tcaggcgtac tgatttctgg
    19981 cttgttaggc ggtatgggcg gtgctgtttt tgctcagtcg atttccggta acttttcagc
    20041 tggtacgatc gttggacaag ggttcatctc catggctgct atgatctttg gtaagtggaa
    20101 tcctttaggg gcaatgggtg cctcattgtt ctttggtttt gctcaaagct tgagtatcat
    20161 tggcgcacaa ttaccagtta tttcttcgat tccgccagta ttgctgcaaa ttgctccata
    20221 tttgctgaca atcattgtgc tggttgtttt cttagggaaa gcttctggtc caaaagcaaa
    20281 cgggaagaac tatatcaaat caaaataatc ttttagcgaa taaaaagggt gggttacggg
    20341 aagtgttttt cgtctcgccc tttttgtttt aaaggcaata aaaataaaaa gaaagaagga
    20401 ataatcatgt ttaaacgcgt acatctgatc gtcatggatt cagtaggaat tggagaagca
    20461 ccagatgccg ataagttcgg tgatgttggt tctgatacat taggacacat tgcgaaagaa
    20521 gcaggattga acgttccaca tttagagcag cttggtttag gaacgatccg tcctcttgaa
    20581 ggtgtggaat cagtgaaaga tcatcaaggc tatgcgacga aattagaaga agtttctgta
    20641 gggaaagata cgatgacagg ccactgggaa atcatgggat tgaatatcaa aactccattt
    20701 cgtgtgtttc ctaatggatt ccctgatgat ctgatcaaac aaatcgaaga attttctggc
    20761 cgtaaagtcg tatgtaacaa accttatagc ggaacagctg ttattgacga ttacggtgaa
    20821 caccaaatga aaacaggcga tttaattgta tatacttctg ccgatcctgt ccttcagatc
    20881 gcggcacatg aagaaatcat tcctttagaa gaattatatc ggatttgcca attcgttcgt
    20941 gatataacaa aagatgatcc gtatatgatt ggccgaatca ttgcacgtcc gtacgtgggt
    21001 gaaccaggag acttcacacg aacaagcaat cgccatgatt atgcgttgaa tccattcgga
    21061 cacacagtcc ttgattcttt gaaagaagca ggaaaggacg tgattgctgt tggaaagatc
    21121 aacgatattt tcaacggaca aggaatcact gaatctgtcc gcacgaaaag caatatggat
    21181 ggcgttgacc agctgctgaa agttatgaaa aacgattttt caggaatcag ctttaccaat
    21241 ttagtagatt ttgatgcatt atacggtcat cgtcgggatg tagtcggcta tgcgcatgcg
    21301 attgaagaat ttgacttacg aattccagaa ttgcttgaag cgatggaaga agacgacttg
    21361 ctaatgatta cagccgatca cggaaacgat ccgacgttta ctggtaccga tcatacgcga
    21421 gaatatgtac cgttattggt ttacagtaaa aaaatgaaag aacaaggggc attgcctcaa
    21481 ggatattatt cagatatttc agcaacgatt gctgagaact ttagtgtttc ttcgactgaa
    21541 aatggcgaaa gcttcttgaa attattaaaa taatcttttt aaataggaga aagcaaatga
    21601 ccaagttaag cgagatgtta caagaaacga cccagtttat ccttgataaa ggagtaaaag
    21661 aaattgattt tggtttgatt ctaggttcag gattaggtga attagcagat gaaatcgaag
    21721 atgctgtaat gatttcttat gatgagatcc cgttctttcc tgtttcgaca gtagttgggc
    21781 atgcaggaaa gctagtttat gggtctctgg caggcaagaa agtgttagcc atgcaaggga
    21841 gattccacta ctatgaaggt cattccatgc aaacagtgac ctatccagta cgtgtgatgg
    21901 ccggactagg ggcccattca gtcattgtga ctaatgcatg tggtggtgtc aacgaatcat
    21961 ttgtaccagg agatttgatg ctgattaccg atcatatcaa ttttactggt gaaaatcctt
    22021 tgatcggtcc gaatgaagat gcaatgggcc ctagatttcc agatatgagt caagcttata
    22081 cgcctgacta tcaagcaaca gcaaaagcag cagcagaaaa actaggtttg gatcttaaag
    22141 aaggcgtata tatgggctat tctggaccaa cttatgaaac gcctgcggag atccgaatgt
    22201 ctcggacaat aggagccgat gcagtaggga tgtcgactgt tccagaagtc atcgtagcag
    22261 ctcacagcgg attaaaagtg ttaggaatct cttgtattac caacttagca gcaggtatgc
    22321 aagcgaatct gagccatgaa gaagttgtag aaacaactca gagagtcaaa caatctttta
    22381 aagcattggt taaagaaaca ttgacattat tgtaatagga ggaacaataa aatgagtgtg
    22441 catattgaag cgaaagaagg cgagatcgcc gataagattt tacttccggg agatcctttg
    22501 cgagctaaat atattgcgga gacttttttg gaagatccag tttgttacaa tcaggtacgc
    22561 ggtatgttag gctttaccgg caaatataaa ggagaacgta tttctgttca aggaacagga
    22621 atgggaatgc catcagccac catctatgcc catgaattga tccagtcgta tggtgtaaag
    22681 aaattgattc gagtaggtac atgcggtgca ttgtcaaaag acgtccatgt tcgtgattta
    22741 gtactagcac aaggtgcagc gaccagttct tcgatgatcg aaaaaaattt ccaagccttt
    22801 cattttcctc caatcagtga tttcaatcta ttactgaaag catatgagat agctaaagaa
    22861 aagggctata cggttcatgt aggaaatgtt ctttcggaag attctttcta caaagatgat
    22921 ttgacagaga cgttccaatt agctgaactt ggcgttttag gagtggaaat ggaagcagcg
    22981 gctctttatt acttaggcgc gaaataccat gtgcagacat taagtttgat gaccgttagc
    23041 gaccatttga tcaccggtga agaaacaaca gctgcagaac gtcaatctac gttcaatgaa
    23101 atgatcgaag taggattgga aacagcgata gcagaataag aaggaaatga aaaagctgcc
    23161 ctgcattaag taataagaac atccaagcaa aaatagctcc tcatgttttt gacttggctg
    23221 gacttattat tgcaaatgta agcttttgaa caccgtttac caagaggttg tgaatctgcc
    23281 gtttaactct gagtcacaag gaacaatttc aaaaaacagc ttttcatgtt tgatgaatat
    23341 tgtgacttgt gaccgagaag ttggcaggag aaaaccgttt atacgaaaaa agggactgtg
    23401 acaaatcgtg tcacagtccc ttttttatag tgttttttat aggtagtatt ttttagcaac
    23461 tgttaagtca tcatttaatt cataaacaag tggttgacct gttggaattt caagatccat
    23521 gatgtcttcg tcagagatac cttcgatatg tttagctagg gcgcgtaatg agttaccatg
    23581 tgcagctact aaaacagttt tgttgtctag taatgctgga gcaatttcat cttgccagaa
    23641 tggcaaagcg cgttctaatg tcactttcaa gttttctcca ccaggaatat cgcgtttgtc
    23701 taacattgcg taacgacggt catttgctgc agaaccttcg tcagtttctt ccattaatgg
    23761 aggaagagta tcgtaagaac gacgccagat gtgcacttgt tcgtcgccgt atttttcagc
    23821 tgtttctttt ttgttcaaac cttgtaattt accatagtga cgttcgttta agcgccatga
    23881 tttgatttgt ggtacccaaa gttgatctga atattctaag atcaagttac atgttttgat
    23941 tgcacgagtt aaaacagaag tgtaagcaac atcaaattcg attccagctt ctttgatttt
    24001 gcgtccgcct tcttttgctt cttcgattcc ttctggtgct agatttacat cagcccaacc
    24061 agtaaattgg ttcaatgcat tccattcact aagaccgtga cgagaaaaaa ctaattttgg
    24121 catgtttagc tctcctttta agataggttt atatttctga aattttcagt tctgttttat
    24181 tgtacaattt tttggaagag atttccatcg aaaaattaga taagattcta tgagaaaaca
    24241 agtagagaat ataaaaaaac gaacagtgcc cacgcaactg ttcgttttta ggtattagat
    24301 gcataattgc tcgatttctt tttcaggtaa attgatatat ttataacatg ttccaactga
    24361 aacaccgcat tttgaagaaa taaattggat cgtttctttt ttttcataat agagacgtcg
    24421 gatttttttg atcgtttttg catcgatttt tggtcttccg ccgatttttc cttttttacg
    24481 agcttcatct aatccaacta atgtacgttc tttgatcaat gcgcactcca tgtttgctag
    24541 gttttccatt aattggaaat agatttttcc catttcgcgc gtatcgattc cttcatcaag
    24601 actaatcaag tgcagcccgc gatctttgaa taattgcgtg agttctgtta attgccgcgt
    24661 agatttgcct aatcgatgaa gttggcaaat gactagtgca tcgccttgtt gcatgtcagc
    24721 gatcactgac tccaaatgaa cagcagtaga atcattttgt tccacttcat atgtctcatg
    24781 gaaaatttct tgacaaccaa agtcagataa ttgatcaacc tgagttgtta aatcaacgtc
    24841 atttatggtt gtgcgtgcat aaccaataat tttcatggtg attcctttct agcagtgatt
    24901 ttctaactgg atataacagt aaaaatgtag cataaagtac gtcaaaaaga tagttttttc
    24961 gcaaattttc cgctgtcttt ttttattgga tgatcaagca gtttttcttt atttacgacg
    25021 gattgcgtca agaatctcaa cgcctttttc tgagccgaca actactttag aaaccatgtt
    25081 taggaaaagc ccatgttcta ctaccccaac aagttcatct aaataggtag ctaagctttc
    25141 tggctgttcg atgacttcta gatgcagatc aatgatgtaa tgaccaccat ctgtggttaa
    25201 gacttctccg ttttcgtctg tacgcaagac gggttggaat cttttttctt caaaaatatg
    25261 aagtagctgt tgggagccat aagggatcac ttcaactggg agtgggaact tcccaagttt
    25321 atcgactaat ttagaagaat caacgatcca aatcgtttct tttgagtacg tagcgactat
    25381 tttctcgaaa agcaaagcag cgccgccgcc tttgatccct tgaaaatcag cactgatttc
    25441 atcagcaccg tctattgtca aatcgactac ggggacttca tcaatactct ttaacggtat
    25501 tcctaatttt tccgcttgtt cttttgtcgc atttgaagta gtaactcctg tgatacgtaa
    25561 accttcttca cgcatacggc gaccaatctc ttctaccata taataggcgg ttgaaccggt
    25621 cccgagacct acgatcatcc cgtcctttac gaatttcgcg gcttcaatgc cagctaattg
    25681 ttttaaattc atctttgcgt ccaccttttt ttagtttgag catcgtgctt tcctcactat
    25741 tttagcgtga tttacggtaa aaagatacca ttctttttta ttccaataaa gctttttcaa
    25801 aaaaaattgc atctcttgtt gacatgtttt ttcattcgtg atattctaac aaaggtgctt
    25861 tatctacagg tttctgtgaa gaaacgtgct gactgtttat caaagcatat ttgataagga
    25921 tgcagacatt gcatttattt tttatcacaa aaaaagaacc acctggatgt gtggaactag
    25981 acgcaaatcg tgggaaggag gaaacaagga atgcctacaa ttaaccaatt agtacgtaaa
    26041 cctcgtaaat caaaggtgga aaaatctaat tcacctgctt tgaacaaagg atataacagc
    26101 tttaagaaaa ctcaaactaa cgtaaactca cctcaaaaac gtggggtagc aactcgtgtg
    26161 ggtacaatga cacctaaaaa accgaactca gctttacgta aatatgcacg tgttcgtttg
    26221 tcaaacttaa ttgaagttac tgcttatatc ccagggattg gtcataactt acaagaacac
    26281 agtgttgtat tattacgcgg tggtcgtgta aaagacttac caggggtacg ttaccatatc
    26341 gttcgcggtg ctttagatac tgctggtgtt aacgaccgta aacaaagccg ttctaaatac
    26401 ggaactaaaa aacctaaagc ttaattttaa ttattaaaca aatgaaactt gcttctacta
    26461 tatagaagta attactatga acaatatttc ggaaggagga gttacggatg ccacgtaaag
    26521 gtcctgttgc aaaacgtgat gttttaccag atcctattta taactcaaaa ttagtaactc
    26581 gcttgatcaa ccgtgtaatg gttgacggaa aacgcgggat tgcagctaac attatctata
    26641 atgcctttga cttgatcaaa gaatctacag gtaacgatcc attagaagtg tttgaacaag
    26701 caatgaaaaa cgttcaacct gttcttgaag ttaaagcacg tcgtgttggg ggttcaaact
    26761 accaagtacc agttgaagtt cgccctgaac gccgcgttac tctaggtctt cgttgggtag
    26821 taaactacgc tcgcttgcgc ggtgaacaca caatggaaga acgcttagca aaagaaatca
    26881 tggatgctgc aaacaacaca ggtgcttctg ttaaaaaacg tgaagacact cataaaatgg
    26941 ctgatgccaa ccgtgcgttt gctcactatc gctggtaaga ttctctctgt ttggtcggct
    27001 tttataagtc gacctcagat agtgtcctta tttttagcaa ctagatagta aagagaggag
    27061 aaacaaggag aatgaccaga gaattttctt tacaaaacac tcgtaatatc ggtatcatgg
    27121 cccacatcga tgctggtaaa acgactgcta ctgaacgtat cttgtactat actggtaaaa
    27181 tccataaaat tggtgaaact catgaaggag cttcacaaat ggactggatg gaacaagaac
    27241 aagaacgtgg tattacgatc acttctgctg ctactacagc acaatggaag ggtcaccgaa
    27301 tcaacatcat cgacacacca ggacacgtag acttcacagt tgaagttgaa cgttcattgc
    27361 gtgtacttga tggtgctgta actgttcttg atgcacaatc aggtgttgaa cctcaaactg
    27421 aaacagtttg gcgtcaagca acaacttatg gtgtacctcg tatcgtattt gcaaacaaaa
    27481 tggacaagat cggtgctgac ttcttatatt cagtatcaac tttacatgac cgtttgcaag
    27541 caaacgctca cccaatccaa ttaccaattg gtgcggaaga cgacttcaca ggaattatcg
    27601 accttgtaac aatgaaggct gaaatgtata caaacgatct tggaacagag atcgaagaaa
    27661 cagaaattcc tgaagaatac cgcgaattag ctgaagaatg gcgcgaaaaa ttggttgaag
    27721 ctgtagctga aactgatgaa gaacttactt tgaaatactt agaaggtgaa gaaatcactg
    27781 aagctgagtt aaaagaaggt atccgccgcg caacagttaa cgtagaattc tacccagtac
    27841 tatgtggttc agcatttaag aacaaagggg ttcaattact attggacgct gttcttgatt
    27901 acttgccatc accgttagat atcccagcta tcaaagggat tgaccctaaa actgatgaag
    27961 aagtagaacg tcctgcagac gacagcgctc ctttctcagc tttagcattt aaagttatga
    28021 ctgacccatt cgtaggtcgt ttaactttct tccgtgtgta ctctggtgtt ttacaatcag
    28081 gttcatacgt acaaaacgca acaaaaggca aacgtgaacg tgttggtcgt atcctacaga
    28141 tgcatgccaa ctctcgttca gaaatctctg aagtttacgc tggagatatc gctgctgctg
    28201 ttggtttgaa agacacaact actggggaca cactatgtga tgaaaagagt ctagttattc
    28261 ttgaatccat ggaattccca gaaccagtta tccaagtcgc tattgaacct aaatcaaaag
    28321 ctgaccaaga taaaatgggt gtggctttac aaaaactatc tgaagaagat cctactttcc
    28381 gtgctgaaac aaacgtggaa acaggcgaaa caatcatcgc tggtatgggt gaacttcact
    28441 tagatatcat cgttgaccgt atgcgtcgtg aattcaaagt tgaagctaac gtaggtgcgc
    28501 ctcaagtatc ttaccgtgaa actttccgtg ctggcactca agccgaaggt aagtttgtac
    28561 gtcagtctgg tggtaaaggt caatacggtc acgtatggat tgaatttaca ccaaatgaag
    28621 aaggtgctgg tttcgaattt gaaaatgcga tcgtcggtgg ggttgttcct cgtgaataca
    28681 ttccagctgt tgaaactggt ttgaaagatg ctatggaaaa tggtgtctta gctggctatc
    28741 cattagtaga tatcaaagct aaactttacg atggttcata ccatgatgtc gattcaaatg
    28801 aaactgcctt ccgtgttgct gcttctatgg cattacgtgc agcagctaag aaagctaatc
    28861 cagtaatcct tgaaccaatc atggctgtag aagttgttat ccctgaagat tatttaggtg
    28921 atgttatggg tcacgtaact gctcgtcgtg gacgtgttga aggtatggaa gctcgcgctg
    28981 gcggccaaca agttgttcgt gcgatggttc cacttgctga aatgtttggt tatgctacaa
    29041 ctcttcgttc tgctactcaa ggtcgtggta catttacaat gacatttgac cactacgaag
    29101 atgtaccaaa atcagtacaa gaagaaatca ttaagaaaaa cggcggaaaa gctgaataat
    29161 ttcttgaaac cggatagtcc tgcaaagaga ttatccaaag agactatatt tttaatatag
    29221 tttcgtgtaa aataattatc gatgatatgg gcatgtggtg taccttgtgt acatcatata
    29281 cctatcagac aaataaaaaa tatattgtat ttttaggagg acattttaaa atggcaaaag
    29341 aaaaatttga ccgttctaaa ccacacgtta acattggtac tatcggacac gttgaccatg
    29401 gtaaaactac attaacagct gcaatcacaa ctgtactatc taagaaaaac ggcggccaag
    29461 ctatggctta cgatcaaatc gatggtgctc cagaagaacg cgaacgcgga atcacaatct
    29521 caacagctca cgttgagtat gaaacagata cacgccacta cgctcacgtt gactgcccag
    29581 gacacgcgga ttacgttaaa aacatgatca ctggtgctgc tcaaatggac ggagctatct
    29641 tggtagtttc tgctgctgac ggcccaatgc ctcaaactcg tgaacacatc ctattgtctc
    29701 gtcaagttgg tgttccttac atcgttgtat tcttgaacaa agtagacatg gttgatgacg
    29761 aagaattact agaattagtt gaaatggaag ttcgtgacct attaacagaa tacgaattcc
    29821 ctggtgacga tgttcctgta gttgctggat cagctttgaa agctctagaa ggcgacgctt
    29881 catacgaaga aaaaattctt gaattaatgg ctgcagttga cgaatacatc ccaactccag
    29941 aacgtgacaa cgacaaacca ttcatgatgc cagttgaaga cgtgttctca attactggac
    30001 gtggtactgt tgctacaggt cgtgttgaac gtggacaagt tcgcgttggt gacgaagttg
    30061 aagttgttgg tattgctgaa gaaacttcaa aaacaacagt tactggtgtt gaaatgttcc
    30121 gtaaattgtt agactacgct gaagctggag acaacattgg tgctttacta cgtggtgttg
    30181 cacgtgaaga catccaacgt ggacaagttt tagctaaacc aggtacaatc acacctcata
    30241 caaaattctc tgcagaagta tacgtgttga caaaagaaga aggtggacgt catactccat
    30301 tcttcactaa ctaccgtcca caattctact tccgtacaac tgacgtaaca ggtgttgttg
    30361 aattaccaga aggaactgaa atggttatgc ctggcgacaa cgtaacaatg gaagttgaat
    30421 taatccaccc aatcgctatc gaaaacggta ctaaattctc aatccgtgaa ggcggacgta
    30481 cagttggtgc cggtgtcgtt acagaaatca aagcttaatt cacttaagtt ttaaagtaaa
    30541 gcaacattta ttcggacgta atcgctcgga acgaaggtct taggatcttt gttccgagct
    30601 tttttattta ttgcaggatc aggaaaaaag acaggagtat tataaataga agaaacacga
    30661 aataaaagag tcagtatttt ctaaatgaaa ctttatgaaa acaaaatgtt ttttacaaga
    30721 aaaagccttg ctttgtgagt ttatatatat tataatgaca aaggtgcgtt tactttagtg
    30781 aattgcacag acgtaatcag aatgatttcg tcggctaaga aacgagaggt tgcgacacgc
    30841 ccggacgctt tgccacgaac gagcgtgacg gaaattttcg tggagctatg tctacttttc
    30901 aaaataggcg aaggagggaa caagatggca aaacaaaaaa ttcgtatccg tttaaaagcg
    30961 tatgaacacc gtattttaga tcaatcagcg gataaaatcg tggaaactgc aaaaagaact
    31021 ggagctagcg tgtctggtcc aattccatta ccaactgaac gcagtcttta cacaatcatc
    31081 cgtgcgactc ataaatacaa agattcacgc gaacaattcg aaatgcgtac acacaaacgt
    31141 cttatcgaca ttgtgaaccc aacaccaaaa actgttgatg ctttgatgaa gctagactta
    31201 ccatctggtg taaacatcga aatcaaatta taaaactaaa acaaaaataa tggaggtgta
    31261 ctcatgacca aaggaatctt agggaaaaaa gtgggaatga cacaaatctt cactgaatct
    31321 ggtgaattaa ttccagtaac tgttgtagaa gctactccaa acgtagtact acaagtgaaa
    31381 acaatggaaa cggatggcta cgaagctatt caagttggtt accaagatat gcgtgaagtt
    31441 ttatcaaaca aacctgcgaa aggtcatgtt gcaaaagcaa acacggctcc taagcgcttc
    31501 attcgtgaat tcaaaaatgt tgagctagga gaatatgaag taggaaaaga aattacagta
    31561 gatgttttcc aagcaggaga cattattgat gtaacaggta ctacgaaagg taaaggattc
    31621 caaggggtta tcaaacgcca cggacaaagc cgcggaccaa tggctcacgg ttctcgttac
    31681 catcgtcgtc ctgggtcaat gggtccagtt gcacctaacc gtgtatttaa aaacaaacgt
    31741 cttgctggcc gtatgggtgg caaccgcgta actatccaaa acttggaagt tgtacgtgtc
    31801 gatgcagaaa gaaatgttat cttgatcaaa ggtaatgttc ctggagctaa aaaatcttta
    31861 attactatca aatcagctgt gaaagctaaa taattgtagg aagaaaggag gaactaagga
    31921 atgccgaatg tagcattatt caaacaagat ggaagccaaa acggtgaaat cacacttaac
    31981 gaagaaatct tcggaattga acctaatgaa agtgttgttt acgatgcaat cgtaatgcaa
    32041 cgtgcgtcat taagacaagg aacacacgcg gttaaaaacc gtagcgctgt tcgcggcggc
    32101 ggacgtaaac catggcgtca aaaaggaaca ggtcgtgcac gtcaaggatc tatccgctca
    32161 ccacaatggc gtggaggtgg cgtagtcttt ggaccaacac cacgttctta cagctacaaa
    32221 cttcctaaaa aagttcgtcg tttagcaatg aaatctgtat tgtcagaaaa agttgctgaa
    32281 aacaacttgg tagcaatcga aggcttgaac ttcgacgcac caaaaacaaa agaattcaaa
    32341 caagttcttg ctaacttatc tatcgactct aaagtattgg ttgttctaga aaaaggtaac
    32401 gactttgctg ctttatcagc acgtaactta ccaaacgttt ctgtagtaac ttctgataat
    32461 gtgagtgtgc tagacgttgt atcaaataca aaagtgttag caacacaaac tgctcttact
    32521 caaattgagg aggtgcttgc ataatgaact tactagacgt aatcaaacgc ccagtgatca
    32581 ctgaaaaatc aatgcttgcc atggatgaca agaaatacac ttttgaagtg gacactcgcg
    32641 caaacaaaac tttagtaaaa caagcggtag aagctgcttt tgacgttaaa gtcaaaaacg
    32701 tgaacatcgt gaacgtacgt gcgaaattca aacgtatggg caaatatgca ggatatacaa
    32761 aaaaacgtcg caaagcgatc gttacattga ctgaagattc aaaagaaatc caattattcg
    32821 aagctgctga ataagcacta atagataact cattaaatta ggagggaaaa acgtggcgat
    32881 taaaaagtac aaacctacct caaatggtcg tcgtaacatg acaggttctg attttgctga
    32941 aatcacaaca tcaacacctg aaaaatcatt gttacaacca ttgaaaaacc atgctggacg
    33001 taacaacaac ggccgcatca ctgtacgtca tcaaggtggc ggacacaaac gccaatatcg
    33061 cgtgatcgac ttcaaacgta acaaagataa cgttgttgca actgttcaaa cgatcgaata
    33121 tgatccaaac cgttcagcta acatcgcgtt agtacactat gaagatggag tgaaagctta
    33181 catcttagca cctaaaggac tagaagtggg tatgaaactt gtttcaggtc cagaagcaga
    33241 tatcaaagtc ggaaatgctc ttccattaga aaacattcca gttggtactg ttatccacaa
    33301 cattgaaatg aaacctggta aaggtggaca attgatccgt tctgctggta caagtgctca
    33361 agtacttggt aaagaaggca aatacgtatt gatccgcttg aactctggtg aagttcgtat
    33421 gatcttagct gcatgccgtg ctacaatcgg ttctgtaggt aacgaacaac acgaattaat
    33481 caacatcggt aaagctggac gctctcgttg gatgcgtaaa cgcccaactg tacgtggtag
    33541 cgtaatgaac cctaatgatc acccacacgg tggtggtgaa ggtaaagctc ctatcggacg
    33601 taaagcacca gtttctcctt ggggtcaacc agctcttggt tacaaaactc gtaacaaaaa
    33661 agctaaatca gacaaactta ttgttcgtcg tcgcaaaaat aaataattac tttgaccatg
    33721 tgtcgcacat tttgagagga ggttcaccat gggtcgtagt ttaaagaaag gaccttttgt
    33781 cgatgagcat ttaatgaaaa aagttgaagc tcaagcaggg gttgaaaaga aaaaagttat
    33841 caaaacttgg tcacgccgtt caacagtttt cccaagtttt gttggatata ccatcgcagt
    33901 ttatgatgga cgtaaacatg taccagttta catccaagaa gacatggtag gacacaaatt
    33961 aggtgaattt gcacctacaa gaacttatcg tggacatgct gctgacgata agaaaacgaa
    34021 acgctaattt gaggggagga taaatccaaa tggcagaaca aatcacatca gctaaagcaa
    34081 ctgcaaaaac agttcgcact tcacctcgaa aagcccgttt agtaattgac cttatcagag
    34141 gtaaaagtgt cgcagatgca atttcaatct tgaaattcac accaaacaaa tctgctggaa
    34201 tcattgaaaa agtattgatg tcagcagttg cgaatgcaga aaataacttt gacttagatg
    34261 ttgaaaactt ggtagtaagc gaagcttttg ttaacgaagg acctacaatg aaacgtttcc
    34321 gtcctcgtgc aaaaggatca gcttctccaa tcaacaaacg tacaagtcat atcacggtag
    34381 tcgtatcaga aaaataagga gggataatca gtgggtcaaa aagtacatcc aattggaatg
    34441 cgtgtaggca tcatccgcga ctgggatgct aaatggtatg ctgaaaaaga gtatgctgag
    34501 ttcttacacg aagatttaag aatccgtaaa tttatttcaa ctaaacttgc tgatgctgct
    34561 gtatcaacag ttgaaatcga acgcgcaaca ggtcgcgtaa acatttcaat tcacacagct
    34621 aaaccaggta tggttattgg taaaggcggt tctgaagtcg aaagcctaag aaaagaatta
    34681 aacaaattaa ctggtaaaag aatccatatc aatatcgtgg aaatcaaaaa accagatcta
    34741 gatgcaaaat tagtaggtga aggaatcgca cgtcaattag aaaaccgtgt tgctttccgt
    34801 cgcgctcaaa aacaagccat ccaacgctca atgcgtgctg gcgctcaagg gatcaaaact
    34861 caagtatctg gtcgtctaaa cggagcagat atcgctcgtt cagaaggata ctcagaagga
    34921 actgttcctc ttcatacttt acgtgcggac attgattacg catgggaaga agcagataca
    34981 acatacggaa aactaggagt taaagtgtgg atctatcgtg gagaaattct tccaactaaa
    35041 aaaaacactg agaaaggagg gaaataatca tgttagtacc taaacgtgta aaacaccgtc
    35101 gtgaattccg cggaaaaatg cgcggggaag ctaaaggcgg aaaagaagta gcattcggtg
    35161 aatacggttt gcaagctgtt gattcacatt ggatcacaaa ccgccaaatc gaagctgctc
    35221 gtatcgcaat gactcgttac atgaaacgtg gtgggaaagt atggattaaa attttccctc
    35281 acaaatctta tactgccaaa gcaattgggg tacgtatggg ttctggtaaa ggggcacctg
    35341 aaggatgggt tgcaccagta aaacgtggta aaatcatgtt tgaaatcgca ggcgttcctg
    35401 aagaagtagc tcgtgaagcg ttacgtctag cttctcacaa attaccaatg aaaactaaga
    35461 tcgtaaaacg tgaggaaatg ggtggtgaat cgaatgaagg ttaaagaaat cagagaatta
    35521 accactgccg aaatgcttga tcaagaaaaa caattaaaag aagaattgtt taatcttaga
    35581 ttccaattag caacaggtca attagaaaac actgcacgta ttaaagaagt acgtaaatcg
    35641 attgcacgca tcaaaactgt gttgcgtgaa caagctaagt aattatggaa ggaggccatt
    35701 aagcgtatga ctgaaggaag aaatcaacgc aaagtttatc aaggtcgcgt ggtttcagac
    35761 aaaatggata aaacaattac cgttgctgtc gaaacaaaga aaaaccaccc tatctatggc
    35821 aaacgcatga actattctaa gaaatacaaa gtgcatgatg aaaacaacac agcaaaagtt
    35881 ggcgacatcg tgagaattat ggaaactcgt ccattatcag ctacaaaacg tttccgtttg
    35941 ttggaagtag tcgaagaagc agttattatc taataacacg agattttcct atatagattg
    36001 aatgcaaaat ctgaaaggag gatacacaac gtgatccaag cagaaagtcg tttaaaagtc
    36061 gctgataact caggcgcacg tgaaattttg acgatcaaag tccttggtgg atctggtcgt
    36121 aaaactgcta acatcggtga tgtgatcgtt gctacggtta aacaagcaac gccaggtggg
    36181 gttgtcaaaa aaggtgacgt cgttaaagcc gttatcgttc gaacaaaatc aggagctcgt
    36241 cgtacagacg gttcatatat taaatttgat gaaaatgctg cagtgattat tcgtgatgat
    36301 aaaagccctc gtggaacacg tatctttggg ccagttgcac gtgaactacg tgaaaacaac
    36361 ttcatgaaga tcgtttcact agcaccagaa gttttataat cttgagacac ttatcaaagg
    36421 aggtgcgaaa cagttatgtt tgttaaaaaa ggcgataaag ttaaagttat cactggaaaa
    36481 gacaaaaaca aagaaggcgt tgtactagca gcgttcccga aaaaagataa agttattgtc
    36541 gaaggtgtaa atgtcgttaa aaaacaccaa aaaccttctc aagctgctcc tcaaggtgga
    36601 attgttgaga tggaagcgcc aattcatgtt tctaacgtga tggtggttga ctctactggt
    36661 gtagctggcc gagttggcta caaagaagta gacggcaaaa aagtccgtgt ttctaaaaaa
    36721 accggtgaag ttttagataa ataaaagttt aaggaaggag ggtcttactg aatgaaccgc
    36781 ctaaaagaaa aatatcttaa agaagtaact ccatcattga tggaaaaatt taactatagc
    36841 tctgttatgc aaacacctaa agttgaaaaa atcgtcatca acatgggtgt tggtgatgcg
    36901 gtatcaaacg ctaaaaactt agataaagca gttgaagaat taactttgat cactggtcaa
    36961 aaaccaatga ttacaaaagc taaaaaatca atcgctggat tccgtttacg tgaaggaatg
    37021 ccaattggtg caaaagttac attgcgcgga gaaagaatgt acgaattctt agacaaattg
    37081 gtttctgttt cactaccacg tgtacgtgac ttccatggtg taagtaaaaa agcctttgac
    37141 ggtcgtggaa actatacttt aggtattaaa gaacaattga tcttcccaga agtagattat
    37201 gatttagtag ataaagtacg tggtatggac atcgttattg taacaacagc aaactctgat
    37261 gaagaatctc gtgagttgtt gacacaatta ggcatgccat tccaaaaata attaaaggag
    37321 gcgaactacg tggctaaaaa atcaatgatt gctaaaaaca aacgtcctgc aaaattttca
    37381 acacaagctt atactcgttg cgaacgttgc ggacgtccac attcagttta tcgtaagttc
    37441 catctttgcc gtatttgctt ccgcgaactt gcctataaag gtcaaattcc cggcgtgaag
    37501 aaagctagct ggtaaaaagt aaattcgcat aagaaggagg taaaagatca atggtcatga
    37561 cagatccaat tgcagatttt ctaactcgta tccgtaacgc aaacatggtt aaacatgagt
    37621 ctttagaagt acctgcatca aaaattaaac gtgacatcgc tgaaatcttg aaacgtgaag
    37681 gtttcgttcg tgatgttgaa tacatcgaag atgacaaaca aggcgtgatc cgtgttttcc
    37741 taaaatttgg taaaaacggc gaacgtgtta tcacaaactt aaaacgtatc tctaaaccag
    37801 gtttgcgtgc ttatgtcaaa gctgacgaag taccaaaagt attgaacggt ttaggtatcg
    37861 caatcatttc gacttctgaa ggtgttgtca ctgataaaga agctcgtgaa aaaaatatcg
    37921 gcggcgaagt tatcgcttac gtatggtaat caagtagaat ctacaaggag gtgtctttaa
    37981 gtgagccgta tcggaaataa agtcgttgtt atccctgaag gagtaacagt tactcaagac
    38041 ggaaacaacg ttacagttaa aggaccaaaa ggtgaattga ctcgtacttt ttcagcagat
    38101 atcaaaatga atatcgaagg aaacgaagta actttcactc gtccaaacga cagtaaagaa
    38161 atgaaaacaa ttcatggaac aactcgtgcg aacttcaaca acatggttgt aggtgtcagc
    38221 gaaggtttcc aaaaagcgtt agaactaatc ggggttgggt accgtgcaca attacaagga
    38281 tcaaaacttg tattgaacgt tggttactca catccagtag aaatcacacc accagctgga
    38341 gtaactgttg aagtaccagc taacacacaa gtaattgtta aaggtgcgaa caaagaagaa
    38401 gttggcgaat tagctgcaaa cattcgtgga actcgtcctc cagaacctta taaaggtaaa
    38461 ggtattcgtt atgttggcga atatgtacgc cgtaaagaag gtaaaactgg taaataatcg
    38521 tttagaaaaa ttgcatagaa gccagcagca ggaaaaaggc atttggctga agtttacctt
    38581 caaaaaaata catttttact tgtgccgcag cttctatttt tcgaaacgta tttactgctt
    38641 gaaatagtag tacaataaaa ataaagaggt gacaattgtg attacaaaac cagataagaa
    38701 caaaacacgt caaaagagac atcgtcgtgt acgtaacaat atctctggta ctgctgagcg
    38761 cccacgcttg aacgtttttc gttctaacaa aaacatctac gctcaaatta ttgatgacgt
    38821 agcgggtgta acgctagcaa gtgcctctac cttggataaa gaaatttcag gtggaacaaa
    38881 aacagaagca gcacaagctg tcggtaaact agttgctgaa cgtgcagctg aaaaaggcat
    38941 taaagtagta gtctttgacc gtggtggata cctttaccat ggccgtgtgc aagctttagc
    39001 tgaagctgct cgcgaaaatg gactagaatt ttagaagaag gaggaatacc attcatggtt
    39061 tatatcgatc caaaacattt ggaattagaa gaccgcgttg ttgcaattaa ccgtgtaaca
    39121 aaagttgtta aaggtggacg tcgtctacgt tttgctgctt tagttgttgt cggtgacaaa
    39181 aacggacacg taggatttgg tacaggtaaa gctcaagaag ttcctgaagc aatccgtaaa
    39241 gcaattgaag atgcgaagaa aaacttagtt gaagtaccta tggttggttc aacaatccca
    39301 cacgaagtaa tcggtgcttt tagtggtggt agaatcctaa tgaaacctgc tgtagaaggt
    39361 tctggggttg ctgctggtgg accagttcgt gccgtattag aattagctgg ggtagcagat
    39421 atcacttcta aatctttagg ctcaaataca ccaatcaacg ttgttcgcgc aacagttgaa
    39481 ggtttgaaac aattgaaacg tgcagaagaa gttgcagaac ttcgcggcaa atcagttgaa
    39541 gaattaatcg gataaggagg acaacaataa tggctgaatt aaaagttact ttaaaacgca
    39601 gtattatcgg acgtcctcaa aaccaacgcg atacagttaa agcgttaggt cttacaaaga
    39661 ttaacagttc agtggttaag ccttctaatg aagcaattaa aggcatgatc aatactgttt
    39721 ctcacctagt ggacgttgaa gaaatttaat ttagaaaaat cataactaga acattaagga
    39781 ggtgccaaac ctatgaaact tcatgaatta aaacctgctg aaggttcacg ccaagtacgt
    39841 aaccgtgttg gtcgtggtac ttcatctggt aacggaaaaa ctgccggacg cggacaaaaa
    39901 ggacaaaaag ctcgttcagg cggcggtgtg cgtttaggat ttgaaggggg acaaacacca
    39961 ttgttccgtc gtttaccaaa acgaggattc acaaacgtga accgtaaaga ctatgcagtc
    40021 attaatcttg atgtattgaa ccgctttgaa gacggagctg aagtaactcc tgttgcttta
    40081 gttgaagctg gaatcgtgaa aaacgaaaaa gctggaatca aagtattagc taatggggaa
    40141 ctaaacaaga aattaactgt gaaagcagca aaattctcta aagcagctca agaagcaatt
    40201 gaagcggctg gtggatcaat cgaggtgatc taatgtttag actattaaaa gatgctttta
    40261 aagtaaaaga cattagatcg aaaatcctat ttactgtatt tgtgttgttt gtatttcgcc
    40321 taggtgcaca tattactgta ccaggcgtaa atgctgcttc attgcaaaac ctttcttctt
    40381 tgccattttt caacatgttg aacttggtaa gtggtagtgc gatgcaaaac ttttccattt
    40441 tctcaatggg ggtttcacca tacattactg cttctatcgt cattcagctt cttcaaatgg
    40501 acatcgtgcc tcgatttgtt gaatggtcga aacaagggga agttggtcgt aaaaaactga
    40561 atcaagcgac tcgatatctg acattagtat tggcatttat ccaatctgtc ggtatcactg
    40621 ctggattcaa tcaatacgct caattaggtt tcgtgaataa tcctaacgta gcaacttacg
    40681 taacgatcgg tttgattcta acagccggta cgatgtttgt tacttggcta ggagaacaga
    40741 tcacagaaaa aggtatcgga aatggcgtat caatgatcat ttttgcaggt atcatttcta
    40801 gattgcctga aagtatcaga gaaatttatg aagattactt tataaatatt gatccttcag
    40861 acatatggaa atctgctatt tttgtggcca ttttactagt agcggtcttg ttgatcgtga
    40921 cattcgtaac atttttccaa caagctgaac gcaagatccc gatccaatat acgaaacgcg
    40981 tagctggcgc gcctacaagc agctatctgc cgttgaaagt aaatgcagct ggagttattc
    41041 cagttatctt tgctagttcg tttattgcta cgccaaatgc tgttttacaa gcattgagcg
    41101 gtagattcgg ttctgaaaac tggtacaaag tgatgatgga gatctttaac tataatacag
    41161 cacctggtgc tacgatttat acgattctga tcgttgcctt cactttcttc tacgcctttg
    41221 ttcaagtaaa ccctgagaaa ttagcggaaa acttgcaaaa gcaaggaagt tatataccaa
    41281 gcgtacgccc tggtaagggg actgaagagt atgtctcaag attactgatg cgtttaagta
    41341 cagttggtgc agtcttcctt ggtttagtgg cattgcttcc aatcattgct caaatggttt
    41401 gggatcttcc acaatcaatc ggattaggcg gtacaagctt actgatcgtt atcggtgtag
    41461 ctttagaaac tgcgaaacag ttagaaggct taatgctgaa acgtaaatat gttggattca
    41521 ttgagtaaga aagcgtgttg aggtttcccc tcaacacacg ttctcacaca tttattagga
    41581 gggaaaacaa tgaacctcat tttaatgggg ctgccaggcg caggtaaagg aacacaagct
    41641 gaaaaaatca ttgatgctta tggtattccg cacatctcaa caggagatat gttccgcgca
    41701 gcgatgcaaa acgaaacagc tcttggcctg gaagcaaagt cttatatgga taaaggtgca
    41761 ttagttcctg atgaagtaac aaatggaatc gtgaaagagc gactagctga accagataca
    41821 gagaaaggct tcttattaga tggctttcca cgtacgctag atcaagcaaa agctctggac
    41881 gcaatgctaa aagatttgaa taaaaaaatt gatgctgtca tcgatatcca tgttggtgaa
    41941 gaaatcttgg tcgaacgtct agccggccga tttatctgca gcaattgtgg agcaacttac
    42001 cataaaattt tcaaccctac aaaagttgaa gacacatgtg atcgttgcgg tgggcatgaa
    42061 ttctatcaaa gagaagatga taaacctgag acggttaaaa atcgtctggc tatcaacatc
    42121 aaaaatagtg aaccaatctt ggcttattat aaagaacaag gtttgctaaa cactattgat
    42181 ggtgctcgtg aaatcgatgc tgtatttgca gatgtacaaa aaatcattga aaaatagcac
    42241 gattgcccgt agccgacttg cattatgcag ttactcttgt caagttcaat aaattatgat
    42301 agaatatgtc agtccgactt ctagagaatc ctctagatga tccattttga gaattttgag
    42361 gtgggaactg cagtttccgc cattttatcg tgtgaaaatg cgaaacaagt acaaggaggt
    42421 actgtgcgtg gcaaaagaag atatgattga agtcgaaggt acagtcgtcg aaactttgcc
    42481 gaatgcaatg tttaaagttg aactagaaaa cggacaccaa gttcttgcta ctgtttcagg
    42541 taaaattcgt atgcattaca tccgtatctt acctggtgac aaagtaacgg ttgaattgtc
    42601 tccatacgat cttaaccgtg gccgtattac ttatcgcttt aaataattgt actccgtaaa
    42661 actacaggag gtattatcat gaaagtaaga ccatcagtaa aaccaatgtg cgaacattgt
    42721 aaagtaattc gtcgtaaagg acgcgttatg gtgatttgcc cagcaaatcc aaaacataaa
    42781 caacgtcaag gataattgga ggtgctataa atatggctcg tattgcagga gtagatattc
    42841 ctcgtgataa acgtgtagta gtttcactta cttatatcta tggtattggt aacactactg
    42901 ctaaaaagat cttagctgat gctggtgtat ctgaagatgt tcgcgttcgt gaattaacga
    42961 atgaacaaac agatgctatc cgtgcagaag tagataaatt aaaagttgaa ggggatcttc
    43021 gtcgtgaagt gaacttgaac atcaaacgtt tgatggaaat cggttcttac cgtggaatcc
    43081 gtcatcgccg tggattgcct gttcgtggac aaaacacgaa aaacaatgca cgtactcgta
    43141 aaggcccagc tcgcgctatc gctggcaaga aaaaataatt cttaagtgaa ggaggttaaa
    43201 acttcatggc agcaaaaaaa gtgaatcgta aacgccgtgt gaagaagaat atagaagcag
    43261 gtattgcaca tatccattct acattcaata acactatcat catgatcact gatactcacg
    43321 gaaatgcatt agcgtggtca tcagctggtt cattaggttt caaaggaagc aaaaaatcaa
    43381 ctccttttgc cgctcaaatg gcagcagaaa ctgctacaaa agcagcaatg gaacacggat
    43441 taaagactgt tgaagtaaca gttaaaggac ctggttctgg acgtgaagca gcaattcgtt
    43501 cattacaagc tacaggttta gaagtgactg caattcgtga cgtgactcca gttcctcata
    43561 atggtgcccg ccctccaaaa cgccgtcgtg tttaatgagt gccgctcatt ttgagtttta
    43621 tcagtaacgt cattgaacaa gacacttttc gttttgaaag gggtaaagat aagaatgatt
    43681 gagttcgaaa aaccaagaat cgcaaaaatt gatgaagaaa aagattatgg caagttcatc
    43741 gttgaacctc ttgaaagagg gtatgggact actttaggga attccctacg tcgtatttta
    43801 ttatcttctt tgccaggtgc tgccatcact aatattcaaa tcgatggtgt actgcatgaa
    43861 ttctctacca tcaaaggtgt tagagaagac gtcactcaaa tcatcttgaa cattaaaggc
    43921 ttagcgctga aaatgtatgg ccaagaagaa aaaacccttg aaatcgatat taccggacct
    43981 gctacagtaa ctgctggcga tattatcgtt gatagtgatg tagaaattct taacaaagat
    44041 atgtacatct gtagtgtctc tgaaggagct acgttccatg ctcgcttaac agtgaaacct
    44101 ggacgtggtt atgttcaagc agatgaaaat aaaaaagaag atatgccaat tggcgttctt
    44161 ccagtcgatt caatctacac acctgttcgt cgtgtgaact atcaagttga gaacacacgt
    44221 gttggacacc gtgaagattt tgataaattg acgatggaga tttggacaga tggttcaatt
    44281 gaaccgttgg aagcaatgag tttgtctgcg aaaatcatga ctgaacattt ggatattttt
    44341 gtgaacttaa ctgatgaagc aaaaaatgct gaaatcatgg tcgaaaaaga agaaacacaa
    44401 aaagaaaaaa tgttggaaat gacgatcgaa gagttagact tgtctgttcg ctcttacaat
    44461 tgtttgaaac gtgcaggaat caatactgta caagaactta caaataaatc tgagcctgaa
    44521 atgatcaaag tccgtaactt aggacgtaaa tcattagaag aagttaaagc aaaattgcac
    44581 gacttaggtt taggtttacg taaagaagat taaaaccttt tacgaaggag ggaaaccaac
    44641 gtgagttatc gtaaactagg acgcacatct agccaacgta aagcgatgtt gcgtgattta
    44701 acaactgatt tattaatcaa cgaacgtatt gtgactactg aagctcgtgc gaaagaagtt
    44761 cgctcaactg cagaaaaaat gatcacttta ggtaaacgcg gagatttgca tgcacgtcgt
    44821 caagctgcgg ctttcgtacg taatgaagtt gccagcgtgc gtgaagaaaa tgaggacatc
    44881 gtcattgaat cagctttaca aaaactgttc aacgatattg cacctcgtta cgcagaacgt
    44941 caaggcggat acacacgcat tttgaagaca gaaccaagac gcggtgatgg tgcaccaatg
    45001 gttgtcttag aacttgtcta aaatcacact aataacatca ctttatagat gattctttta
    45061 gagtgttatg atgttggaag atatttttcc gagtctagct cagcgctgct cctcaagcga
    45121 atatcgcttg gggagtaagc actcatcata aagtgttttt ttataccttc aattacctgt
    45181 catgacagat atgtttgaag gcataaaaaa acattttaag cgagaaataa ttgaaaattt
    45241 tgtctttttt tgatagtgtt agtaggaact ttaggatgaa gaaggagtaa tcatggagcc
    45301 aattattgaa ttagaaaaga taaattataa ataccagcca gatgatcttc gccccgcatt
    45361 gaaagatgtc tcctttacaa ttgataaagg tgagtggatc gctattattg gacacaatgg
    45421 ttcaggaaaa tcgacacttg ccaaaacgat caatggattg cttctgccag aatcagggat
    45481 cgtgaaagtg ggaaatcaaa tattggatga agaaaatatt tggacgatcc gacagatggt
    45541 tggaatggtt tttcaaaatc cagacaatca gtttgttggt tcgacagtag aagatgacgt
    45601 tgcttttggt cttgaaaatc aagggattcc cagagaagaa atgctagtgc gtgtgaaaga
    45661 tgcacttgag aaagttagaa tggctgagtt tgcttcacgc gagcctgctc gtttatcagg
    45721 agggcaaaaa caacgtgttg ccattgccgg cgtggttgcc ttacgacctg atatcattat
    45781 tttagatgaa gcaaccagta tgctagatcc agaaggtcgt gaagaagtga tctctacgat
    45841 caagaagatc aaagaagaaa gtcaattgac cgttatatct atcactcatg atattgatga
    45901 agcagctaat gccaatcgga tactagtgat gagacaaggc gaacttgtcc gtgaaggtac
    45961 accaaaagaa attttttctg ctggtcctga actgatcgac ttaggtctag atctaccatt
    46021 tcctgaaaaa ctgaagagcg cactgaaaga acgtggggtt gatgtgccca gtgaatatat
    46081 gacagaagaa aggatggtgg attggttatg gacatccgtt ttgaacaagt agacttcact
    46141 tatcagccga atacaccatt tgaacaaaga gccttatttg acatcaatat gacgatcaaa
    46201 gaaaacagtt atactgcatt agtagggcat accggaagcg ggaaatcaac cttacttcag
    46261 catttgaatg cgcttgttaa gccaacaagc ggtacagtcc atattggaga acgagatatt
    46321 cagccggata cggataataa gaatttaaag cccatccgaa aaaaagttgg catcgtcttt
    46381 cagtttccag aagcacagct attcgaagaa acggtagcaa aagacattgc tttcggtcct
    46441 aaaaactttg gtgtcagcga agaagaagca ctagtcctag caaaagaaac attagaacaa
    46501 gttgggctgg atgaaagcta tttggaacgt tcgccatttg aactttcagg agggcaaatg
    46561 cgtagagtag caatcgctgg tgtgcttgcc atgaggccgg aagtacttgt attggatgaa
    46621 ccaacggcag gacttgatcc ccagggcaga aaagaaatga tggagatgtt ttggcgtctt
    46681 cataaggaac agcagatcac tattgtatta gttacgcatt taatggatga tgtagccaat
    46741 tttgcagatt atgtctatgt gttggaaaag ggacgaatcg ttaatagcgg ggagccgcaa
    46801 gaggtctttc aaaacattga atggctaaaa gaaaaacaac taggcgtacc tactgccacc
    46861 gagtttgcag aagaattaat ggctaaaggg atgaactttg ctaccttgcc tttgacagct
    46921 gaagaattag cagacgctat cgtccattat gctggaggaa atgcccatga tgaataaatt
    46981 gatatttggt cgttatatgc ctggagattc cttgatccat cgattggatc cccgagctaa
    47041 attattagcc agtttttatt ttatcggaat cattttttta gcaaataatt gggaaacgta
    47101 ccttttgttg gcatttttca cattactttc tgtcttcttg tcaaaagtag atttgaaatt
    47161 ttttatccga ggcattcgcc cattgatctg gctaatcctc ttcactgtag ctttgcaggt
    47221 cttgtttaca agtggcggag aaacttattg gagttggggt gttttccata tcactgaatt
    47281 tggtgtgatg aacggcctct ttattttctg tcgattcgtg ttgatcattt ttatgtcgac
    47341 tctactcacg cttactacac cgcctttgga attatcggat gcgattgagt acttgcttcg
    47401 tccattgaaa gccgtacggt tcccagtcca cgaaatttca ttgatgctgt cgattgctct
    47461 tcgttttgtg cctacattga tggacgaaac agaaaagatc atgaacgctc agcgcgctcg
    47521 cggtgttgat tttggtgaag gaaacctagt tcagaaaatg aaagcagtca ttcctttgct
    47581 gattccttta tttgtcagca gcttcaaccg tgcggaagat cttgcgactg ctatggaagc
    47641 aagaggctac aaaggtgggg aaggaagaac gaaatatcgt gtattgcatt ggcataatag
    47701 agacacgatg gttatcattg cttttgtatt attaactgtg ctactgatct ttttaagagg
    47761 ataaacgttc agtgggccgc tatctattct tttcttgctg atctgtttgc aagaaaggat
    47821 gcaagcagtg tatgacaaga aatattaggg tctgtggcag actaaggtca caggcctctt
    47881 tcttttatct gaaaagaaag aacgaaaaaa ttttatagaa aaatcattat tccagataac
    47941 cttttaggaa caaaggagag aaaaacatgg tacgttacaa agcaatcatt gcgtacgacg
    48001 gaacaaattt taatggattc caaaaacagc caaatgggcg tactgttcaa gaagaagtag
    48061 aaaaaacatt acaaaaaatg gcaaatggaa aagaaatcac ggtctttggt tcgggaagga
    48121 cagatgccgg agtacatgcg atcgggcaag taatccattt tgattatcca gaggagcgtc
    48181 cgttagaacg aatgcgtttc gcgctagata cccagtcacc agaggatatt gctgtaagac
    48241 aagtagaaat tgtttcagaa gacttccacg caagatattt ggtcaaagaa aagacgtatc
    48301 aatttcgagt ggatataggg aaaccacgca gtccttttcg acgccattat gcaagttatt
    48361 ttccttaccc acttgatttg gaaaaaattc agcgggcgct tcctgattta ttagggactc
    48421 acgatttcac ctctttttgt gcatcaggaa gctcgataga ggataaaata cgtacgatct
    48481 acgaggctaa gatggaggta aatgaaacaa aagacgaatt gttgtttaca tttaggggaa
    48541 atggcttctt gtataagatg atacggatct tagtaggaac gcttttgaaa attggcaatg
    48601 gccgattgcc tgaagatagt atcccagata ttattgcgaa aaaagatcgt aatgcagcag
    48661 gacctacagc acaccctgaa ggactttatt tgtatgaagt gaaatatgaa taagaggtta
    48721 tgatagaagt ggtcagcttc aagaaataag aaggtattta cgaaaattac ttctcaaaat
    48781 tttgcaaaat ttcggcttat tttcgaagaa gctacttctg gaacaccgtt taaggaagag
    48841 gtcgtgaaat cagcgttttg acctgagtat tagagaaaca aacgggaaaa tagctcctca
    48901 tattttgccg tttgttgagc taataccgaa ggtcagctga ttgaacaccg tttaagaaag
    48961 aggtcgtgag cctgtcattt aactctgagt cacaaagagc aatttcaaaa aacagcttct
    49021 catctttgat gaatatcgtg acttgtggac gaggcgttga taagtgaatc atctttttgt
    49081 tacctatttt ttctgtaacc aaaaaacgat ggaacgttgt tttgattgcc cttggtgcgg
    49141 acagtctgaa caggttccat cgtttcttta ttttctacgt cttcttctgt gtgtgtttcg
    49201 tggttgttct tctattgctg gatcaatcgt gccataaaag gggcgattgc ggtcaaggta
    49261 ccagacataa agaataaaca atgcaataca taggacaaaa aggattgcac ctatcgtaaa
    49321 tccttcaggc aagtaactga aagtgacagt atgtttacct gctggaactt ttatcatcaa
    49381 gaatgcgtct ttgaaattgc tgaccttgac tggtttccca tcaattttag ctttccatcc
    49441 tttgtcataa ggaatagttg tcaccaacat ggaatcttca gcggtattta cagtcccaga
    49501 agcttttctg ccagtggctt ccatttcgac acctttttct ttgatctgat tcattgcggc
    49561 ttgataggct acaacatcta gaccaacgac tttaggctcc atgaaactta tttctttcgt
    49621 cccataaaaa cttgcagtaa atgtcactgt tgtctcttga ggataatagc caagatcata
    49681 gtattgacca gaaatgttga tttgcgtttt tcgcgatgta ccattgacag tgatcgtcgc
    49741 actagaactt tctaattgcg caaaattggt tgggaacaaa cttaagtaag cttgtgtatt
    49801 tgcaggcaca tggacttccc aagtgagctc ttttggctgg ttatctgctg tttctgagta
    49861 agtgacgcca gtcgttgtct ttgtgatcgt cgtattcaac tgattcttca aagtaggtgt
    49921 atagaatgta aaatactgtt catttagacc agacaatgta ttgatcaagt ttgtctgact
    49981 ggtcaaatta tctgtcactg gctgctcaac atcattaacg gacaacggtg cagtaaatgc
    50041 tagtggcaat gcattgacat tctgatacag tgtatagtcg ccgctttgac cagcttggcg
    50101 gaagccatat ttactgaacg tgctttcgtt tttagaaatg ttatatttga ttcctgtaaa
    50161 cccatccatc agcaaagtat tattgttata acggatattc aaatttgtcc ctcttgaacg
    50221 gaaacctaat ttatccaggt aagaggaaga attacgattt cttatagaag aaaacaaact
    50281 gataccactg taaccatagt tgatcgaatc gtttgcagat actggatcca gattttccaa
    50341 gcggtaaaac gagtcggaat ctttttttgt ttcatttaca agagctttca agtcaggata
    50401 aggatcagta tataaactgc gggaagcata attccaatca tcaagaatgc catggatcat
    50461 actgttcgta ttgaccagtg cttcagcaga catgatcaat aataacaata tactcagatg
    50521 attgaatgaa agttttttat gctgataaaa tcccacagca aaagcataaa tccctaagaa
    50581 aaccactgtc aatacaaagg aagtcatcgt cacataggtg tagcttcctt caggtttgat
    50641 gccccatgcc accgcaaaga gagcagctac gaccaaaatg attcctaaca acagcccacg
    50701 atcgtcttgt tctatctttt cccatccttt tcctgcaagc aggagcacta agaaagagaa
    50761 cagataggca taacggaata aaaacatatt cggtgcatgc ataccatgcc agaacaaatt
    50821 caatggaaca agataaaaac ttgcgataag tatggcgaaa agactgccgt acaaaaattt
    50881 atttttcaac ggtactttcc gactggcaaa ataaaacaga cagaggacta atggtagcag
    50941 acctgcgtaa acgaacggaa tcgatccgta ttttgtcgtg tcataaacac caatcatatt
    51001 tttcatgatt agatccagat aagaagtcgc ttctgttttc aacgtagtga tctgactcag
    51061 tgcctcgcca tttgaccgaa gatccagcac tgctggcaat acaatgatca ttgaagcgcc
    51121 gcctgccagt aaagaagtga taccataagg aacaatcgtt tttttatatt ctgaccagtt
    51181 tgtcaataga cgggcgataa aatataagaa agaaaatacg ccaatcataa aacccatata
    51241 aaaactggat aaaaacaaga gtaaataact gataaataaa agtttcggcc gtttaagatc
    51301 cataacacga tgaatcccca aaatcactaa tggaagataa aaaaatgcat ccagccacat
    51361 gatgatctcc gaatgtgcag tgataaatga gatcaatgcg taactgacgc taagtgcaac
    51421 atgcccccag cgaggaatct tgaacgtacg tgcaagaaac caaaaagcca atccggcaca
    51481 gccgacctta agcaaagtca gaacatacag tgcatctggc atcatctggt ttggaaaaaa
    51541 gaacaccagt ggcgtgaaga tcccgcctag atagtaagaa attaaagata aataattcaa
    51601 tcctagagaa gcattccatg tataaaaaat actttgcttt ccaagaagaa catttcggaa
    51661 gctggcatga aaattagaaa actgtgaaaa ggcatcactt gccaaaatac ttctgctgct
    51721 tccaggatag attccgatac ttagataaac aagtgctaaa atcaaaaacg gaatcaggaa
    51781 actagctgct gcgtagcgtc cgttttctct aaagaatgac tttaaatttt ttttcatatt
    51841 tattttccac caaatttcta tgaagttgta ttcagtttat catatccttg tttttctctg
    51901 cttaaaaaga gtctagtttt cacaaaaaaa taaaatttag aataaataac ttcataattt
    51961 gttcgtataa caaaaataaa cgcaaaaaaa gggtaggaat caaaagtccc acccttaaaa
    52021 atttttatta aataaaaaac cgttcactat ctgaacggtc caagtatcat tcaattaaac
    52081 gcgttcaact tttcctgatt tcaaagcacg agttgacacc caaacttttt taggttttcc
    52141 gtcgaccata acgcgaactt tttgtaagtt aggtttaaca gtacgttttg ttgagttcat
    52201 agcgtgtgaa cggttgtttc cactttttgt cttgcgacct gtaaagtaac aaacttttgc
    52261 catggtaatt tcctcctttg ccttgatttg gagcaataga ataattcagc tcataccaga
    52321 ctaacatatc atatagaatg ggaatttgca agtacatttt catagataca attgacaatt
    52381 gagaagaata attgtaaatt aaacaaggtt atacctgaaa ttttgcaagg tttttgagta
    52441 ctttgggtaa ctgcctttta tttcaagttg cgctatgata aaatatgata gaagaaaaat
    52501 gggagttttt tacccgttaa ggaggctcat tcatggctgt aaaaattcaa acgtcagctg
    52561 gaaccattga aattaccaat gatgtaattg caacagttgt tggtggcgcc gcgacagatg
    52621 tgttcggtgt tgtaggaatg gccagcaaaa atcaaattaa agataatatt actgagattt
    52681 tgcgtaaaga aaattactca agaggagtag ttgtccgcca agaagataat ggtattgctg
    52741 tagatgttta tacgattatc agctatggta cgaaaatctc tgaagtttcc cgcaacgtcc
    52801 aagaaaaagt gaaatacaat ctagaggcat tattaggcgt aactgccaat tcagttaatg
    52861 tctttgtaca aggtgttcgt gtactgccag actaggcagg agactggctg gtaactcagc
    52921 agtcgatctg aaggaggatt atctaggtga gtgtaacaga aataagtgca agccagttcc
    52981 aggaaatggt ccaggcaggt gcgaatcgtc tgcaagtaaa tgcagaatac gtcaattcat
    53041 taaatgtatt ccctgtgcca gatggcgata cgggaacaaa tatgaatcta tctatgacta
    53101 gtggagctaa ggcagtagta gattcaacct cagaaaaagt aggagaatta gcagcaatgc
    53161 tttcaaaagg attgctgatg ggtgcgcgcg gaaattccgg cgttattttg tctcaattgt
    53221 ttcgtggatt ttctaagcaa attccagatg ttgtctcatt aaatgcttct gatcttgctg
    53281 cagccttcac tcatggagtt gaaactgcgt ataaagcagt aatgaaacca gtagaaggaa
    53341 cgattttgac agtagctaga gaagcagcga aagctggaga aaagaaagca aaaagtactg
    53401 atgatgtcgt tgaagtaatg acagctgttg taaaaggagc aaagcgtgct ttagctaaaa
    53461 ctccagactt gcttccagtc ctaaaagaag tcggtgtcgt agacagtgga gggcaaggac
    53521 tgctgtttgt ttatgagggc tttttaagtg cattgaatgg agaattccaa gcggatgaaa
    53581 catatgaacc ttctccagca gaaatggatg atatggtcaa tgcagaacat catcgcagtg
    53641 tccaaggtca aatggcgaca gaagatatcc attttggtta ctgtacagaa atcatggtaa
    53701 aaatcggaga aggacctaca gtcgatagta cattcgatta cgaagaattc cgtaactatt
    53761 tagatggtat aggagattct ttacttgtgg tcaatgatga cgagatcatc aaagtccacg
    53821 tgcatacaga aaacccagga gaagtcttga attatggaca aaaattcggt tcattagtca
    53881 aaatcaaagt cgacaacatg cgcttgcagc atgagacgat tttggaacac gatgaagaag
    53941 tgaacgagtt tgcgaaaaaa gaaataccac gtatcccgta tggtatcgta gcgatcgctg
    54001 ctggtaaagg cgtccaagag ctgtttgaaa gtttaggcgc taattatgtt atcagtggtg
    54061 gacaaacaat gaatccaagt acggaagaca ttgttaaagc gattcaagaa gtaaacgcag
    54121 ataaagtgat tgtcttgcca aacaataaga acatcttcat ggcagctgat caagcagcag
    54181 aagtagcaga tatcccagta gcagttgttc cttcaagaac gatttcccaa ggattgacag
    54241 ctatgctttc attcaatgat caagcttctt tagaagaaaa caaagaaacg atgacagaaa
    54301 tgttgtctag cgttgtgagc ggtcaagtca ctcacgcgat cagagatacc tcaattgacg
    54361 gtgtagagat caaagaagga gactttttgg gcatgatcga tggaaaaatc ctgatctcta
    54421 acccagatat cacagcaaca gcgttaagta cactagaaca aatgatcaat gaggacacag
    54481 agatcgtaac gatcttggca ggtgatgaag gaacagttga acaagcaaat gaatttgctg
    54541 agaaactaac agaaaaacat cctgatttgg aaatcgagat ccaccaagga gatcagcctg
    54601 tctatcctta tctattttct gctgaataat ttctgtgtca gaatatcttt ctaacacagt
    54661 attcattcat aaaaggcctt cggttagtga tcaggtatga tcgctgacca agtccttttt
    54721 ttgttaaagg agggaggaaa aatttatgcg ttctttagat gatccaatca ctatgctggc
    54781 aggagtcgga gaaaaaagag cagctagtct tgccagcttg ggcatccaaa cgattgaaga
    54841 cttactgact tattatcctt ttcgctacga agatattcag gaaagaaatc taaacgagat
    54901 ccaggatcaa gaaaaggtta ctttgaaagg aatcgtagtc tcgcctcctg tgatgaatcg
    54961 atttggttat aaaaaaagcc gattgcaatt tcgtatgatg caagaacatg atgtgttcaa
    55021 cgtttctttt tttaatcaac cttatctaaa agaaaaagtc attttatcgg aggaaatcgc
    55081 cgtatacggt aaatgggatg caaagcgtaa agccttgaac gggatgaaga ttttaggttc
    55141 tcaaacaatg gatgactttt cgccgatata tcacgtcaac aaatcaattc gtcaaacgac
    55201 attagttgat ctaatcaaaa aagggtttca agaatacggc acattgattg aagaaaatct
    55261 gccaaacaac cttgtggaga aatatcgact gcttgaccga gcttctgctg taagaagtat
    55321 gcattttcca aaagaccacg aagaaaacca tcaagccaaa cgacgtgtcg tttttgaaga
    55381 gttcttttta tttcaaatgc gtcttcaagg attgaaaaaa gcagaaaaag cagaaacgaa
    55441 tggtttggag atcctttatg atgtccaaaa attgaaacaa tttactcaaa agctaccttt
    55501 tgaactgaca aatgcacaaa aacgtgtaac gaatgaaatc tgtcgggatc ttcgcagtcc
    55561 gcagcatatg cagcgacttt tgcagggaga tgtaggtagc ggaaaaacag tggttgccgc
    55621 gattgcgtta tacgctaccg ttacagcagg tttccaaggt gcgttgatgg tgcctacaga
    55681 aatcttggca caacaacata tggaaagctt gcaacagctg tttgatccca atgaagtaac
    55741 catcgcattg attacggggt caacgaaagc aaaagaacga cgtgagctcc ttgaaaaact
    55801 agagcaaggg gaaattaacg tcgtgatcgg cacacatgca ttgatccaag atggagtcga
    55861 atttcagaat ttaggtctag ttattacgga tgagcagcac cgttttggcg tgaaccagcg
    55921 gaaagtactt cgtgaaaaag gctggcgacc agatgtctta tttatgacag cgactccgat
    55981 tcctagaaca ttagctatta cagcatatgg tgagatggat gtatctatca ttgatgaact
    56041 tccagctgga cgtatcccga tcgaaacacg ctgggtaaga acacctcaat tggacagtgt
    56101 actggattgg acgaaaaaag aactggcaag aggacatcaa atgtatgtca tctgtccgct
    56161 gattgaagaa tcagaagcat tagatgtaaa aaatgcagtg gaaatctatg aaaaacttcg
    56221 tgaactattt gcaccaaaat atgaagttgg cttgttacat ggtaaaatga agaatcagga
    56281 aaaagaagta atcatggaag ccttcaaaga caaccagatg cagatattag tttcgactac
    56341 tgtaattgaa gtaggggtaa acgtgccgaa cgcgaccgtc atgctgatca tggatgcgga
    56401 tcgatttgga ttggcacagc tgcaccaact tcgtggacgt gttggacgcg gaagtgatgc
    56461 ttcctactgt atcttagttg ctaatcctaa aaatgaacta ggtgtggaac ggatgaaaat
    56521 catgacagag accaccaatg gttttgtact aagcgaaaaa gatctggaat tacgtggtcc
    56581 tggtgaagta tttggcttcc gtcaatctgg tctgcctcaa tttgcgattg ctgacttggt
    56641 gacggatgga aatgttttgg aggtagccag agaagaggct gcagcccttt ggcaaataaa
    56701 ggattggcag atgcttcctg agtatcagcc tttagcgcaa agcctccaaa tagaaaatca
    56761 ggataatcgt tatttcgatt agcagactgt gctatacttt atactgatga cggaaaaagg
    56821 agtggaaaca atgaaaattg ccgtagatgc aatgggcgga gatcacgcac ctcaagcaat
    56881 tgtagaaggc gtggctttag cgcagcaaga ttttccagaa gtagaattct tactttacgg
    56941 aaaagaagca gaaatcaaga aatacttaac aaatgaaaaa aacatcacga ttatccatac
    57001 agacgaaaaa atcaatagtg atgatgagcc agtaaaagca attcgacgaa aaaagactgc
    57061 atcaatggtc cttgctgcac aagcagtgaa aaacggtgaa gcagatgctg tattttcagc
    57121 agggaataca ggcgcgctac tagcagcagg gttgttcatc gtaggacgta tcaaaaatat
    57181 cgaacgccct ggcttgatgt ctaccttgcc agtagtaggg aaagaaggtg ccggtttcga
    57241 tatgctggat cttggcgcta acgcagaaaa caaaccagaa catttgttgc aatacggtat
    57301 tttaggttct ttttatgcac gtaaagtccg aggtatcgga cgtcctcgtg tcgcactctt
    57361 aaacaacgga acagaagcaa caaaaggtag tgaagtgacg aaaaaagcct atgaactcct
    57421 tcagaatgaa acaagcttga attttattgg aaacgtagaa gctcgtgagc tactcaatgg
    57481 tgtggctgat gtagtggtca cagatggatt tacaggcaac gctgttttga aatctattga
    57541 aggaacagca atgaatatga tgaatctatt aaaatctgct attctaaatg aagggatcaa
    57601 aggaaaaatg ggcgctatgc ttttaaaaga cggcttacgt tctttgaaag cagaaatgga
    57661 ttattccaaa catggcggtg ccgttttgtt tggcttgaaa gcaccagtca ttaaaactca
    57721 tggagcaaca ggccccgatg ctgttcgcta tacgatccgc caaatccaca caatgcttga
    57781 gacagatgta gttggacaat tagtcgaaca atttgaaaaa agcgaagaat agtatttgga
    57841 tatctgaagc gatccgttcc attgaaaaaa aggtggacat tcctgaaaaa tgccagtaaa
    57901 attagccatg gtgtaggtaa ttgacaagtg tatgattctt ggaggtgaaa aagttgactc
    57961 gcgaagaagt atttaacaaa gtagcgaaaa ttatttcaaa tcattttgag atagataccg
    58021 ataaagtgac ggatgaattg aacatcaaag atgatttaaa agctgattca attagtatta
    58081 tggaattcgt tttagagctt gaagatgaat tcggtacgga aatttcagat gaagacgctg
    58141 aacaaattga aacagtcggt ggcgcagtag attatatcag cagccatctt aaataatttc
    58201 aaataaaagc aaagaaggat aaaaagctgc atgcagcttt ttatccttct ttttttaatc
    58261 tttttgacac gtaaaagtca tttttctgat aattctggcg ataaacagaa attatgttgc
    58321 aaagtaaagc ggttttctat ataattatta ataattcatt acacaaagat aagaataacc
    58381 tttgttataa aacgatttat tctgtgtaat gtcctttttt attaagaaaa acttccattt
    58441 aggaagcaga gaggggtatt cgattgtccg ataaccaatt attagaagtt cagaacctac
    58501 atacaggatt ccgactcaaa gatgactatt acgatgcagt agatgacgtt tcatttactt
    58561 tagacaaaaa cgagatttta gcaatcgttg gggaatctgg gtgcgggaaa agtaccttgg
    58621 ctactacgat tatgggatta cttgatccta ataacacaaa aattacaggt gagattttat
    58681 ataacgatct gaatttaatc gacctgaacg aaacgctcta taataaaatc cgcggaaatg
    58741 atatcggaat gattttccaa gatccgttgt ctgcattaaa tcctctcatg agaatcgaag
    58801 accagatcaa agaaggactt tcctatcaca caaagatgaa tgcagaacag cgtcaagctc
    58861 gtgcactaga attgctagaa caagtaggga ttcctaatcc tgcgagagtt ggacgccaat
    58921 atccccatga actatcaggc ggtatgagac aacgtgtcat catcgcaatc gcaatcgcct
    58981 gcaaacctcc aatcgtcatt gcagatgaac cgacgacagc cctagatgta accatccaag
    59041 cacagattct tgatttactg aaagaccttc aggaagaaac aaaaacaggg atcatcttga
    59101 tcactcatga cttaggagtc gtagcagaaa tggctgatcg agtggcagtg atgtatggcg
    59161 gacagttcgt tgaagtagca ggtgtaaaag aactgtttga tcatcctaag catccttata
    59221 cacgctcttt actgaactct ataccacaag aaggaaatca tgatgcagaa ctccatgtga
    59281 tcgaaggtgt cgttccttct ctgaaaaaca tgcctcgtaa aggttgtcgg tttgcaccac
    59341 gtattccatg gataccagca agcgatcatg aagaagagcc tatccttcat gaagtcgcac
    59401 cgaaccactt ggtacgttgc tcctgctata agcatttcca ctttagagac gaaaaaggag
    59461 aggtgtagat gacagaatta atcacaatca aagacctgaa agttcattac cctattcgca
    59521 gtggattctt caatcgagta acggatcatg tatatgctgt agatggtgtg gattttatta
    59581 tcgaaaaagg aaagacatat ggtttagtgg gagagtctgg ctctggaaaa tctactacgg
    59641 gaaaagccgt tgtgggatta gaaaaggtaa cagccggaca aatcatgtat gaaggaaaag
    59701 atgtaacgaa gcgaagcaac cgcaaaaaaa tgggttataa caaggatgtg cagatgatct
    59761 tccaagattc aatgtccagt ttgaatccta aaaagcgagt gttggatatc attgctgaac
    59821 ccatccgaaa ttttgaacgg ttaagcgatc aagaagaaaa gaaaaaggta aaaggactgc
    59881 tagatatcgt tgggatgccg gaagatgcgc tatataaata tcctcatgaa ttttcaggcg
    59941 gacaacgtca acgtctagga gtagcccgtg ctgttgctac caatccaaaa ctgatcgtag
    60001 ccgatgaacc tgtatctgct ctggatctat ctgttcaagc gcaagtattg aatttcatga
    60061 aacgaatcca gcaagaatac ggcctcagct atttattcat ttcacatgat ttaggtgtag
    60121 taaaacacat gtgcgacaat atcgcaatta tgtacaaagg tcgatttgta gaaatcggta
    60181 caagagaaga catttataat gacccgcgac atatctatac gaaacgtctg ctttcagcta
    60241 ttccgcaaat cgatgtagaa aatagggaaa gccacaaaga aaatcgacga agagtagaac
    60301 aagagtatat ccaaaaccaa aaagaatact atgatgcgac tggtcgagtc tacgatttgc
    60361 gtacgatcac aactacacac aaagtggcgt tgaaagatgg aggtgcaagc taatgtggaa
    60421 aacgattctg cgacgtgttt tattgatgat cccgcaagtt attattttaa gtgttctgat
    60481 cttcttatta gcccaagcga tgccggggga tccatttacc ggtttgatca atccaaatca
    60541 ggatccggca gtgatcgaac agatgcgaca agcagctggc ttgaatgacc catggtatgt
    60601 ccagtacttc cgttggatcg gaaatgcttt gcaaggcgat tttggtcaaa gtttcttata
    60661 caaacgctct gttgctgact tgatcggcga acggatcatc aacactttat atctatcgat
    60721 cttgacagtt gtccttactt atttgattgc tgtgcctctg gggatgcttg ctggccggta
    60781 tcaaaattca atcttggata agattgtcgt aatctacaac ttctttagtt ttgctgttcc
    60841 tttatttatc ttcggcttga tcatgctatt tgtttttggt tatcgtttag gctggttccc
    60901 aacaagtgga tcacaaacac taggtttgca aggtggattt atcactcaat ggatagaccg
    60961 tttgaaatat attctgttac catcaattac ccaagcattt ttggcgactg ccgtcacaat
    61021 ccaatatctg cgaagtgaag taatcgattc gaaatcgctc gattttgtca gaacagcccg
    61081 ttccaaaggg gtaccgacga ataaagtatt cagtcgacat attttccgaa atgcagcgtt
    61141 gccgatggct tctcaaatgg ggtatgaaat cacttcgttg attgctggtt ctgtagttat
    61201 cgaaaagatt tttggttatc cgggagtcgg aaaactattt attgattcca tcggccaacg
    61261 ggactatacc gtgatcacat ccttagtatt gattttagga atcgcgacgc tagtggggac
    61321 attactttca gatatcatca tgagtatcgt tgacccgcgt attcggattc aataaaacca
    61381 atcagaaagg agaatggatc gatgagtgaa aaaaacaata cagcagttca agaaagtatt
    61441 cctcctatgg ggatacgaat gattgctcga gaatttaaaa aagataaaat tgctattttt
    61501 tcattaggtt tgctagtggt tttattatta gtgatcttta tcggtgcgat gctgaccgat
    61561 caagacaaag tgatgtatgt tagtatcttc gacaaatacg ctgcaccagg aacagtttcg
    61621 acacaaggac aaaagttcat tctgggagca gatgaaggcg gacgtgatgt attaggacaa
    61681 ttgatcatag gtgctagaaa ctctgttacg atcggatttg cgatcacgat ccttacttcg
    61741 atcatcgggg ttggattagg gatcatctct ggctactacg gagggatcat tgacaacatc
    61801 ttgatgcgga tcgttgattt tatcatgatt ctaccgatca tgctgatcat tatcgtcttc
    61861 gtatcgatta tttccagata caatgtctgg tcctttatat tcatcatgag tgcattttat
    61921 tgggtagcta aagcccgatt attccgaagc aagacattat ctgaagcaag aagagattat
    61981 gtcagtgcct caaaaacaat gggaaccaat gattttaaaa tcatgttccg cgaaatcatg
    62041 ccgaatctaa gttcattaat cattactaat ttgacgatga actttgcggc aaacatcggt
    62101 atcgagacaa ctttgacttt cttaggtttc ggcttgccgg caaatgtacc tagtttaggt
    62161 acattgatcg gttatgcaag taacggagaa gtcttagtga atcggcaatg gatctggtta
    62221 cccgcatcaa tccttatttt agttttgatg ttgagtatca attatgttgg acaagcattc
    62281 aagcgttctg cagatgcgcg ccaacggtta ggctagtata gaagcgaagg gggaaatagg
    62341 aatgaagaaa acgatttttg gatttgcagc gctgatgtct gctgtcgttc ttgcagcatg
    62401 cgggggcgga ggtacagata cctctggaaa caactctgga ggcaacggca ataacaaaga
    62461 ggaattaact tttccaattg caacaaaaaa taatgatgat gcaatcgaag acggcgaatt
    62521 agaagctgcg gtcgcaacgg acagtcagtt taaaggattg ttccagtggg agttttacca
    62581 agattcatat gatgcagctt ttatggctcc ttctcatgaa ccgctatttg caaacgatga
    62641 cgatttcaga atcacagacg acggagctgc aacgcttgat ctagatgaag atacgaaaaa
    62701 agcgacaatc aaagtaaaag acaacgtcaa atggtcagat ggtgaagatg taacggcgga
    62761 cgatgtgatt ttcccttacg aaatcattgg gaataaagat tacactggta tccgttatga
    62821 cgacactttc cgtaacatcg taggtatgga agaatatcat gatggaagtg ccgacacaat
    62881 ctccggaatc aaaaaaatag acgacaaaac agttgaaatc gaatataaag aagtaaaccc
    62941 aagcatgctt caatcgggtg gtggtatctg gtcatatgca gcaccaaaac atacgttaga
    63001 aggtgtagca atcaaagata tggaatcaag cgatcaagta cgtaaaaatc cagtgacttt
    63061 tggcccttat tacatgagta atatcgttgc aggagaatca gtagaatact tgccaaacga
    63121 gcactactgg aatggtacgc ctaaattgaa aaagatcacg atgaaatctg taccgatggc
    63181 aagtgcaact gaagcattga actctaaatt atatgatatg attttccaaa tgccaacaga
    63241 tacttacgat acctataaag aaattgctgg ctatacaaac ttaggccgtg aacaaacttc
    63301 ttatacttat ctaggattca agttaggtaa atggaatgca gatacaaaga gtgtggaata
    63361 caatccagat tcaaaaatgg ctgataaatc attacgtcaa gcaatgggct atgcattaga
    63421 taataatgca gtaggtgaac gtttctatgc aggtttgaga agcaatgcga cttcattgat
    63481 tcctccagta ttccagtcat accatgatga tgagatgaaa ggattcacac aagatctaga
    63541 caaagcgaaa aaactattag atgatgctgg ctacaaagat gtggatgacg atggtttaag
    63601 agaagacaaa gatggaaata aactaacaat caactttgct tcgatgtccg gtggggaaac
    63661 agcgcaacca ttggcagatt actatatcca acaatggaaa gaaattggtt tggatgtcca
    63721 atacacaaca ggtcgtctga tcgaattcaa ttctttctat gataaattag aaaacgatga
    63781 tcctgaaatt gatgtttacc aagctgcttg gaatacagga actgatccaa atccaagtgg
    63841 actatgggga ccaaatgctg cgttcaacta cactcgttat gaatcagaag aaaatacaaa
    63901 attgatcaac gatatcagtt caagcaaagc atttgatgaa gagtaccaaa aagaagcata
    63961 tcaaaaatgg caacaatatg catttgaaga agcatttgcg attccaacgt tgttccgtaa
    64021 tgaagtattg ccagtcaacg accgtgtcaa agactgggat tggtcttatg atgtaccaaa
    64081 cccatgggca aatgtttctg ttacagcaga ttcacgttca taatcactaa aaatgaccga
    64141 aaaagggcga tgtccttttt cggttttttg atatttttct gtttatttct ctaatattca
    64201 ggtcaaggcg ctgatttcat gacttctcca ataaacggtg ttcaaagtcc agtttctcgg
    64261 tattaagtca aatttatgca aaaatatgga aagccatttt cacaaaattt tttcttcata
    64321 ctcgaaacta aacgaatttt tcacaacttc tcgtcaatca ctaggattat taaagcttta
    64381 cttacctttt gaccgaacca tttcaaattt gacaaaaaac cagtataata aggaagtatc
    64441 gaaaaaatat ccataaaagg aatgtggaaa atggacaacc agctgacaaa agaactaaaa
    64501 gaaaactata acatcgtttt tcacgatcta aatttgcttg aacaagcttt tacccattca
    64561 tcctatgtga atgagcatcg caatctgcaa ttatcggata atgagcgact tgaattttta
    64621 ggagatgccg ttttagaact gatggtttct cagtatttat tccgtttata tcctcatttg
    64681 ccagaaggaa aattgacaaa aacacgagct gcgatcgttc gcgaagacag cttgtcgaaa
    64741 tttgccaaag aatgtcactt tgatcaatat gttttgttgg gcaagggaga agagaattca
    64801 ggaggacgta cgcgaccagc attgctttgc gacctattcg aagctttttt aggcgcgtta
    64861 tatcttgacc agggattcga cacgacccat gcattcatcg aaaaagtgat ttttcctaaa
    64921 gtcaaagcag gtgctttttc acatgagatg gatcataaaa caaaactaca agaagtcttg
    64981 caaaaatcag gtgatgtatc aatcgaatac cgattgatca atgaagaagg tccagctcat
    65041 gaacgagtat tttggatcga agtttatgta gatgatcaat tgattggcac tggacaaggg
    65101 aaatcgaaaa aattagcaga acaagctgcg gcggaaaatg cattggcagc actgtaaaag
    65161 taagtgaggg aaagctttgt atttgaaacg aattgaaata gccggtttta aatcatttgc
    65221 agacaagaca gtcattgatt ttgaaaatag cgtcacagca gtggttggac caaatggcag
    65281 cggaaaaagc aatattacag aggctatccg ctgggtatta ggagaacaat ctgctaaaag
    65341 tctgcgaggc ggaaaaatgc cggatatcat ctttgccggt tccgataccc gtaagcagct
    65401 gaatattgca gaagtcacag tgattttaga caatagtgat cattatctgc cattggagta
    65461 caatgagatc agtgtaaccc gccgttatcg acggactggg gaaagcgagt ttttcatcaa
    65521 taaacaaagc tgcagattaa aagatatcca agaattgttc ttagattctg gattaggaaa
    65581 agaatcgttc tcaatcattt ctcaaggaaa agtagaagcg attttcagta gtaaaccgga
    65641 agaccgtcgt gggatctttg aagaagcagc tggtgtcttg aaatacaagc agcggaaaaa
    65701 gaaagcagaa cagaagcttt ttgaaacaga agacaacctc agccgcgtcc aagacatcat
    65761 ccacgaattg gaagaacagt taactccttt ggccgcccag agtgaggcag caaaggagtt
    65821 tttacggcta aaagaaacac tgactcaaac cgatgtctct ttgatggttg ctgaaatcaa
    65881 gacagcaaaa aaagattggg acaacaaaca agcacagctt gcgaaattca atctagaatt
    65941 aggcaaacta tctgagtcga tccaagaaca agaatccatt ttggcgaagc agcgtaaaga
    66001 aaatgcgcaa gccgatcgtc tgatcgaaaa aaatcagcaa gtattattag atctttcgga
    66061 aaaactgaag cagacagaag gacaaaaaga tgttttgcag gagcggacaa agcatacaca
    66121 aaaaagctcg caagaatatc aaacttcatt agcagaagca caaaaaaagg ttaaacattt
    66181 tgaaaagctt caagaaagct tgatgaaagc agcagcagaa aaagaaacag agatccaaaa
    66241 agctgaagca aatctcataa aaacacagca agaattagaa aaataccaaa aatcaacgaa
    66301 agaattattg gcagaattaa gagatcaata tgttgatttg atgcaagaac aagcagctgt
    66361 aggaaacgaa ctgaagtatc tagaacgtca atatatacaa gaaacagcaa aaagcaagca
    66421 gacgttagcc aagcaatcgg aagtagaagc atcagtagac cgattgatct tacaaaaaga
    66481 agaattgaca caaaagcagg cgcagctaaa atcatcttta actgaaacaa aagagaagtt
    66541 agaaatgatc caacaaaacg ggaaaaagtt ccaagaagca ttagcaaaag aacagcctaa
    66601 aatgtatcaa ttaatgaatc aagtccagca gctacgtgct cgtaagaaaa gccttcaaga
    66661 aatccaagaa aactattttg gtttttacca aggggttcga ttggtcttgc agcataaaca
    66721 gcagctttcg ggtattgtcg gagcagtggc tgaactgatc gatgttcctg cagatttcac
    66781 gttagctatc gaagcagctt taggtggggc agcccagcat gtcatcgttg aaaatgaaaa
    66841 agatgctcgg caagccatca cgtatttgaa acagcaaaga ggaggaagag caacattcct
    66901 tccactgacg acaatcaaac caagacaatt accggctcat atattgacac aagcagcagc
    66961 tgtagaaggg tttataggga ttgccagtga acaagtcagt tatcccgatc aaatccaaac
    67021 tgtggttcaa aacctgctag gaacgatcct tttagccaaa gacttaacta gtgccaatgc
    67081 aatcgcgcaa acgatccgct atcaatatcg agtggtttcc ttagaaggtg atgtgatgaa
    67141 tgccggagga tccatgacag gtggagcgaa taaacgagga aaccaaggca gtttgtttgt
    67201 acaaaatcaa gaattgaaac agttgactag tgaatttgaa gaagcagata aacaactcca
    67261 agcacaagaa aaaaaagttc aggagctgca acaagaaaca gctcgtttag ccgaagaaca
    67321 agaagttttg cgtacacgag gcgaacaatt acgttttgaa gaacaagaag cgacaaatca
    67381 gctacaaaac atcataaatg aacttgaacg ttttgaaaaa gaaaagcaaa tctcaacttt
    67441 tgagacacgt gaactgcagc agtttatcga agattacgaa aagcaaacga acgaattgaa
    67501 agacaaacaa acagatcttg aatcgcaacg acaaaaaatc gacgaagaaa tcaaatcgtt
    67561 aagtcaagaa agcgaccaga tggaagccag aagagcacaa gtacaaagtc aaaaagcaca
    67621 agaacaagca gatcttgctg tcttgaaaga gcagtttaat catctgcaga tccagctccg
    67681 cggtgcccga gtccaaaaag cagaggcaac cgaacgtcaa gaagcgatag aaaagcagtt
    67741 ggcgacattg acagcagact tttctgatca tgaagtcaca gaagaaagtt tggaaaaaca
    67801 aattaacgaa ttatctgctc agcgagaaac attaaaagcc gagttggcaa aagccaaaga
    67861 gcagcgagat agaaggcaaa aggagatcga taagcttgaa gcagtcttgg cagagcgtaa
    67921 tcgtgagcaa aaagcacgac tgtcggaaca atccaaatta gaagtccaaa aagaccgagc
    67981 tgaaatgatt ttagataatc atttgaccta tctgcagtca gaatatcagc tgacatttga
    68041 gaaagcttcc caagactatc aagaaacaac cgatatcgaa gacagtagaa caaaggtttc
    68101 atccttaaaa gaacagattg aaaaactggg accagtaaac ttaaattcaa tcgaacaata
    68161 cgaacaagtc agtgaaagac atacattctt agcaacacag cgagatgatc tgttagcagc
    68221 gaaaaaccaa ttgtttgaaa cgatggatga gatggatgat gaagtgcgga caagattcaa
    68281 agaagtattt gaagctatcc gtcaagaatt caaaattgtc tttccaaata tgtttggcgg
    68341 cggtaaagct gaattagtac tgacggaacc aactgatttg ctgaaaacag gtattgagat
    68401 tgaagtacaa cctcctggca aaaaactgca aagtctaagt ttgctttctg gaggagaacg
    68461 agcacttaca gccatcgcgt tgctcttttc cattatccgt gtctgtcctg ttccgttttg
    68521 tatcttagat gaggtagaag cagcgttaga tgaagccaac gtgaaacgtt ttggtcgtta
    68581 cttgagcgat ttccaagatg acacacaatt tatcgtagtc acacaccgca aaggaacgat
    68641 ggtcgcagca gatgtactgt acggagtgac gatgcaggaa tccggtgtat caaaaatcgt
    68701 ttctgtacgg atggaagata tcaatgaaga ggcgaaaatc aacgaaacga aagtgtagga
    68761 acgaaaatga tcaaattagt agcaatcgat ttggacggta cattattaaa tgaacaaaag
    68821 ctgatttcag atgagaataa acaagcatta gcacaagcaa aacagcaagg ggtcaagatc
    68881 gttctatgta caggacgccc cttagctgcg atggcgcatt atctgcaaga attaggactg
    68941 gtagatgaag gtgactacag catcactttc aacggcggtt tggtacaaaa aaatgatact
    69001 ggtgaaatca tggaaaagaa agtgatggaa gtatcagata tccaacgctt atacaaactg
    69061 gctcagcaat tggatctgcc actagatgtt ttgtctgaca atgtggtttt acagctgcct
    69121 agtgccccaa ggaagcaatc cttgtacaat gtgttgaata agctgctgca attccaacca
    69181 gcggatataa gcgatattac agatgaattt gtgcttaata aggcagtaat cgcttatgac
    69241 caagaagaat tagatcctaa aatcaaagaa ataccagcag aattccatga aatgtatgag
    69301 atcatcaaaa caagaagtat gcttttggaa tttatgccaa aaggtgttac aaaagcttat
    69361 ggtatttctt tattggcaaa agatcttggt ttgaaacaag aagaaatcat ggcgatcggt
    69421 gatgaggaaa atgatttacc gatgatccaa tatgctggac taggcgtagc gatggagaat
    69481 gctgtgcctt tcgtcaaaga agcagcagat ttcgtgacag ccagcaatga agaaaatggc
    69541 gtagccaaag tgatccaaaa atttgtatta gagtaggggg agtcaaatgg gattttttga
    69601 taaaatcaag cgtgctttta ctggcgaaga gaaaacagaa gaagttgaag tacaggagaa
    69661 gtacgacaag ggtttagaaa agacgcgtaa aacatttggt gaacggatga atgagttgtt
    69721 tgccaatttt cgaacagtcg atgaagactt ttttgaagaa ttagaagaaa cacttatcgg
    69781 tgcagatgta ggttttgaaa cagcgatacg aatcaccgaa gcattacgtc aagaagtgaa
    69841 actcagaaat gcgaaaaaat cggcagaagt ccaaaatgcg atcatcgaga aacttgtgga
    69901 tctctatgaa gcggaaggaa tcaatgaagt aaatgaacta aatatccaag aagacggact
    69961 aacagttatt ttgtttgtcg gtgttaatgg tgtagggaaa acaacaagta ttggtaaact
    70021 tgcccatcag ttcaaaaacg aaggtaaaaa agtattgatg gcagcagcag ataccttccg
    70081 cgctggagca atcgaccagt tagttgtctg gggcgaacgc gctggtgttg aagttgttcg
    70141 tggcaatgca ggaggcgatc cagcagctgt cgtattcgat gctttggaaa gagcaaagac
    70201 agagcaggca gatgttttac tagttgatac tgcaggccga ctgcaaaata aagtgaactt
    70261 gatgaacgaa ttagaaaaaa taaaacgagt aatccagcgg gaattaccag aagcacccca
    70321 agaaacactt cttgtattag atgcaaccac tggtcaaaac gcgatggttc aagcaaaaca
    70381 attcaaagaa acaacagatg tgaccggtct tgttttgaca aaattagatg gtacagcaaa
    70441 gggcggtatc gtacttgcta tccgtaatga attgcattta ccagtgaaat tggtcggtct
    70501 tggtgaagga atcgatgact tggagccgtt tgatccaaat gattttgtcg ttggattgtt
    70561 caaaggatta ctaaaagaag aataacccat gtctaaaaag caggagctgt gacaaaagtt
    70621 gcagctcctt gtttgaatga ttgtttttaa catagctgtg tttttgatgc cttcattttt
    70681 atacttattt gtaagcggtt ctcaaacaat ttcgttcttt ttcttagact ttttgattat
    70741 aattagattc atatgaattg gaggtcactt aatgatgaat tttaagcaat tattattgca
    70801 tgtcaatgcg cgccctttta tcgaccaagc tcgatttggt atagaacggg aaggacagcg
    70861 ggttgatctt gcaggaaatc tagcaaaaac cgatcatcca gcaatctttg gcgatcgatc
    70921 ctatcacccc tatatccaaa cagattttag cgaaacacaa acagagatga tcacccctgt
    70981 taccgattct attcccgaat tatttcagta tctcgctgct gtttatgatg tgactgctcg
    71041 ttctataccg aaagaagaga tgatctggcc attaagtatg ccacctgcct taccggaaaa
    71101 agacgaagag atcattattg caaaattaaa aaatttcgaa gatgtcttgt atcgacgtta
    71161 tttagcaaaa gaatacggga agcgaaaaca gatggtaagc ggtattcatt tcaattttga
    71221 atttggcgat gagttgctaa gaacattgtt cagccatcag gaagaatttc aagatttttc
    71281 cgaattcaaa acagaacttt atttgaaaac agcgagaaac tttatgcgct atcgctggat
    71341 gatcacttat ttattcggtg cttcaccaat gagtgagaag aactactttt tagatgaatc
    71401 acatccgcaa gaacctgttc gcagtattcg caacagtgcg ctgggctata cgaatcatcc
    71461 aaatgtgaaa gtttcctatg cttctatgaa acagtattta gcagatatcg agcgaatgat
    71521 cgaagaaggg aaactttcgg aggagaaaga attttataca ccgcttcgtt tccgaggcgg
    71581 aaagaaagtc gcagatttag caacaacagg tgttcgttat attgaattgc ggaatatcga
    71641 tttgaatcct tatgcaagat tgggtatcaa tccagagcaa gttcggttct tacaattatt
    71701 cctgatgtac atgttatgga cagaagaaaa agaagactgt gaccaatggg tagcagaagg
    71761 tacgactcga aataacaaag tagcactaga acaaccatca gatcaaacag aatttcatca
    71821 agaaggtaga gaaattcttg aaggaatgaa gcaaatgctg gttgaattgg attggttgga
    71881 ttctctttat ttagtagagg aagcattgac tcaaatggat catcctgaac aaacgttagc
    71941 agcgaaactc tatcaagaag cacagctatc gagtcagcaa gaagttgctg tggcattggg
    72001 acatcaatat tataaagaaa gccatgaacg tccctatcag ttggccggtt ttcgcgaaat
    72061 ggaactttct actcagatat ttatgttcga tgccatccaa aaaggtgtcc aagtcaaagt
    72121 attagatgag tcggatcaat ttttgcgtct gcaattccaa gaccatgtag aatatgtgaa
    72181 aaatgcaaac atgacaagta aggacagcta tatcgtccca cttatcatgg aaaacaaaac
    72241 agtaacgaaa aaagtcttaa aagaagctgg gttccgggta ccaggaggcg cagaattttc
    72301 atccatggaa gaagcggtaa aagcttatcc gagatttgcc gagcaggcat ttgtgatcaa
    72361 acctaaatca accaattacg ggttaggtat cacgattttc aaggaaggag ctagtctgga
    72421 agattatcaa gcaggtttag ccattgcttt tcgtgaagac agttcggttt tagtagaaga
    72481 atttatgccg ggaacggaat atcgtttctt tgtgatcgac ggagaagtcc aagccatcat
    72541 gttgcgagtc cctgctaatg ttattggtga cagtatccga acagtgaaag aacttgttga
    72601 agaaaaaaat agtgaccctt tgcgtggaac gaatcatcgt gcacctttag aattgatcca
    72661 attaggtgaa ttagaacaat tgatgttgaa agaacaaggg ttgacgatcg aatcagttcc
    72721 ccaagccaat caaattgttt atctaaggga gaactcaaat atcagtacag gtggtgattc
    72781 gatcgatatg actgatgaat tttctgaagc ttacaaaaaa atcgccgttt ctgcggtaga
    72841 agcactagga gctaagatta gtggtatcga tttgattata ccagataaag aaatcgatcc
    72901 tacaactgat aaaaaagcat atgggatcat cgaagcaaat ttcaatccag ccatgcatat
    72961 gcatgtttat ccttttgcag gaaaaggcag aagattgacg atgaatgtat tgaaattgtt
    73021 atatcctgaa gtattttaaa agaaaaagct tttgacagat tgtcc
//
LOCUS       DAZUKC010000002        71166 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000002 DAZUKC010000000
VERSION     DAZUKC010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 71166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.002"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..482)
                     /gene="mreD"
                     /locus_tag="ACG80T_000079"
     CDS             complement(<1..482)
                     /gene="mreD"
                     /locus_tag="ACG80T_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFS4839768.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     P"
     gene            complement(486..1340)
                     /gene="mreC"
                     /locus_tag="ACG80T_000080"
     CDS             complement(486..1340)
                     /gene="mreC"
                     /locus_tag="ACG80T_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFS4839769.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(1495..2133)
                     /locus_tag="ACG80T_000081"
     CDS             complement(1495..2133)
                     /locus_tag="ACG80T_000081"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFS4839770.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(2134..2784)
                     /gene="rpe"
                     /locus_tag="ACG80T_000082"
     CDS             complement(2134..2784)
                     /gene="rpe"
                     /locus_tag="ACG80T_000082"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFS4839771.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(2797..3696)
                     /gene="rsgA"
                     /locus_tag="ACG80T_000083"
     CDS             complement(2797..3696)
                     /gene="rsgA"
                     /locus_tag="ACG80T_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFS4839772.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(3861..5930)
                     /gene="pknB"
                     /locus_tag="ACG80T_000084"
     CDS             complement(3861..5930)
                     /gene="pknB"
                     /locus_tag="ACG80T_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFS4839773.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(5927..6667)
                     /locus_tag="ACG80T_000085"
     CDS             complement(5927..6667)
                     /locus_tag="ACG80T_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFS4839774.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(6680..8038)
                     /gene="rsmB"
                     /locus_tag="ACG80T_000086"
     CDS             complement(6680..8038)
                     /gene="rsmB"
                     /locus_tag="ACG80T_000086"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFS4839775.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(8038..8976)
                     /gene="fmt"
                     /locus_tag="ACG80T_000087"
     CDS             complement(8038..8976)
                     /gene="fmt"
                     /locus_tag="ACG80T_000087"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFS4839776.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(8969..9460)
                     /gene="def"
                     /locus_tag="ACG80T_000088"
     CDS             complement(8969..9460)
                     /gene="def"
                     /locus_tag="ACG80T_000088"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFS4839777.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(9475..11883)
                     /gene="priA"
                     /locus_tag="ACG80T_000089"
     CDS             complement(9475..11883)
                     /gene="priA"
                     /locus_tag="ACG80T_000089"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFS4839778.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(12136..12441)
                     /gene="rpoZ"
                     /locus_tag="ACG80T_000090"
     CDS             complement(12136..12441)
                     /gene="rpoZ"
                     /locus_tag="ACG80T_000090"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFS4839779.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(12446..13060)
                     /gene="gmk"
                     /locus_tag="ACG80T_000091"
     CDS             complement(12446..13060)
                     /gene="gmk"
                     /locus_tag="ACG80T_000091"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFS4839780.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(13547..13831)
                     /locus_tag="ACG80T_000092"
     CDS             complement(13547..13831)
                     /locus_tag="ACG80T_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4839781.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(13849..14739)
                     /locus_tag="ACG80T_000093"
     CDS             complement(13849..14739)
                     /locus_tag="ACG80T_000093"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFS4839782.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(14759..14977)
                     /locus_tag="ACG80T_000094"
     CDS             complement(14759..14977)
                     /locus_tag="ACG80T_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFS4839783.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(14947..16647)
                     /locus_tag="ACG80T_000095"
     CDS             complement(14947..16647)
                     /locus_tag="ACG80T_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFS4839784.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            16914..17687
                     /locus_tag="ACG80T_000096"
     CDS             16914..17687
                     /locus_tag="ACG80T_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4839785.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(17768..18337)
                     /gene="thiT"
                     /locus_tag="ACG80T_000097"
     CDS             complement(17768..18337)
                     /gene="thiT"
                     /locus_tag="ACG80T_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFS4839786.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(18403..18505)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18511..19482)
                     /locus_tag="ACG80T_000098"
     CDS             complement(18511..19482)
                     /locus_tag="ACG80T_000098"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFS4839787.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(19576..20949)
                     /gene="glmU"
                     /locus_tag="ACG80T_000099"
     CDS             complement(19576..20949)
                     /gene="glmU"
                     /locus_tag="ACG80T_000099"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFS4839788.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(21452..22270)
                     /gene="purR"
                     /locus_tag="ACG80T_000100"
     CDS             complement(21452..22270)
                     /gene="purR"
                     /locus_tag="ACG80T_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFS4839789.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(22437..23261)
                     /locus_tag="ACG80T_000101"
     CDS             complement(22437..23261)
                     /locus_tag="ACG80T_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFS4839790.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(23234..23914)
                     /locus_tag="ACG80T_000102"
     CDS             complement(23234..23914)
                     /locus_tag="ACG80T_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFS4839791.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(23937..24875)
                     /locus_tag="ACG80T_000103"
     CDS             complement(23937..24875)
                     /locus_tag="ACG80T_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFS4839792.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(25120..25971)
                     /gene="ispE"
                     /locus_tag="ACG80T_000104"
     CDS             complement(25120..25971)
                     /gene="ispE"
                     /locus_tag="ACG80T_000104"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFS4839793.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(26125..28698)
                     /gene="mprF"
                     /locus_tag="ACG80T_000105"
     CDS             complement(26125..28698)
                     /gene="mprF"
                     /locus_tag="ACG80T_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFS4839794.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            28839..29645
                     /gene="proC"
                     /locus_tag="ACG80T_000106"
     CDS             28839..29645
                     /gene="proC"
                     /locus_tag="ACG80T_000106"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFS4839795.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(29724..31280)
                     /gene="rny"
                     /locus_tag="ACG80T_000107"
     CDS             complement(29724..31280)
                     /gene="rny"
                     /locus_tag="ACG80T_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFS4839796.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(31691..32740)
                     /gene="recA"
                     /locus_tag="ACG80T_000108"
     CDS             complement(31691..32740)
                     /gene="recA"
                     /locus_tag="ACG80T_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFS4839797.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(32849..34090)
                     /locus_tag="ACG80T_000109"
     CDS             complement(32849..34090)
                     /locus_tag="ACG80T_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFS4839798.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(34330..34908)
                     /gene="pgsA"
                     /locus_tag="ACG80T_000110"
     CDS             complement(34330..34908)
                     /gene="pgsA"
                     /locus_tag="ACG80T_000110"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFS4839799.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(35117..36040)
                     /locus_tag="ACG80T_000111"
     CDS             complement(35117..36040)
                     /locus_tag="ACG80T_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFS4839800.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(36779..38065)
                     /locus_tag="ACG80T_000112"
     CDS             complement(36779..38065)
                     /locus_tag="ACG80T_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFS4839801.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(38058..39326)
                     /locus_tag="ACG80T_000113"
     CDS             complement(38058..39326)
                     /locus_tag="ACG80T_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFS4839802.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            39578..40039
                     /gene="mscL"
                     /locus_tag="ACG80T_000114"
     CDS             39578..40039
                     /gene="mscL"
                     /locus_tag="ACG80T_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFS4839803.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(40098..40544)
                     /locus_tag="ACG80T_000115"
     CDS             complement(40098..40544)
                     /locus_tag="ACG80T_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4839804.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(40637..40882)
                     /locus_tag="ACG80T_000116"
     CDS             complement(40637..40882)
                     /locus_tag="ACG80T_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFS4839805.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(40997..41575)
                     /locus_tag="ACG80T_000117"
     CDS             complement(40997..41575)
                     /locus_tag="ACG80T_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS4839806.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(41652..42182)
                     /locus_tag="ACG80T_000118"
     CDS             complement(41652..42182)
                     /locus_tag="ACG80T_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFS4839807.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(42186..43046)
                     /gene="rlmB"
                     /locus_tag="ACG80T_000119"
     CDS             complement(42186..43046)
                     /gene="rlmB"
                     /locus_tag="ACG80T_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFS4839808.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(43039..43434)
                     /locus_tag="ACG80T_000120"
     CDS             complement(43039..43434)
                     /locus_tag="ACG80T_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFS4839809.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(43431..44843)
                     /gene="cysS"
                     /locus_tag="ACG80T_000121"
     CDS             complement(43431..44843)
                     /gene="cysS"
                     /locus_tag="ACG80T_000121"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFS4839810.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(44840..45376)
                     /gene="epsC"
                     /locus_tag="ACG80T_000122"
     CDS             complement(44840..45376)
                     /gene="epsC"
                     /locus_tag="ACG80T_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFS4839811.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(45462..45701)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(45698..47158)
                     /gene="gltX"
                     /locus_tag="ACG80T_000123"
     CDS             complement(45698..47158)
                     /gene="gltX"
                     /locus_tag="ACG80T_000123"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFS4839812.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(47328..48485)
                     /locus_tag="ACG80T_000124"
     CDS             complement(47328..48485)
                     /locus_tag="ACG80T_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFS4839813.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(48519..49889)
                     /gene="radA"
                     /locus_tag="ACG80T_000125"
     CDS             complement(48519..49889)
                     /gene="radA"
                     /locus_tag="ACG80T_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFS4839814.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(49952..50479)
                     /locus_tag="ACG80T_000126"
     CDS             complement(49952..50479)
                     /locus_tag="ACG80T_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFS4839815.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            50709..51515
                     /gene="proB"
                     /locus_tag="ACG80T_000127"
     CDS             50709..51515
                     /gene="proB"
                     /locus_tag="ACG80T_000127"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFS4839816.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            51512..52759
                     /locus_tag="ACG80T_000128"
     CDS             51512..52759
                     /locus_tag="ACG80T_000128"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFS4839817.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            52835..53179
                     /locus_tag="ACG80T_000129"
     CDS             52835..53179
                     /locus_tag="ACG80T_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4839818.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(53296..53622)
                     /locus_tag="ACG80T_000130"
     CDS             complement(53296..53622)
                     /locus_tag="ACG80T_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4839819.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(54237..55439)
                     /locus_tag="ACG80T_000131"
     CDS             complement(54237..55439)
                     /locus_tag="ACG80T_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4839820.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            55556..56071
                     /locus_tag="ACG80T_000132"
     CDS             55556..56071
                     /locus_tag="ACG80T_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS4839821.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(56270..56716)
                     /locus_tag="ACG80T_000133"
     CDS             complement(56270..56716)
                     /locus_tag="ACG80T_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFS4839822.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(56763..58241)
                     /gene="msr(C)"
                     /locus_tag="ACG80T_000134"
     CDS             complement(56763..58241)
                     /gene="msr(C)"
                     /locus_tag="ACG80T_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFS4839823.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(58595..59224)
                     /locus_tag="ACG80T_000135"
     CDS             complement(58595..59224)
                     /locus_tag="ACG80T_000135"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFS4839824.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(59208..59672)
                     /locus_tag="ACG80T_000136"
     CDS             complement(59208..59672)
                     /locus_tag="ACG80T_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS4839825.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(60159..61427)
                     /locus_tag="ACG80T_000137"
     CDS             complement(60159..61427)
                     /locus_tag="ACG80T_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFS4839826.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(61658..62395)
                     /locus_tag="ACG80T_000138"
     CDS             complement(61658..62395)
                     /locus_tag="ACG80T_000138"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4839827.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(62462..63031)
                     /locus_tag="ACG80T_000139"
     CDS             complement(62462..63031)
                     /locus_tag="ACG80T_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFS4839828.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            63159..64031
                     /locus_tag="ACG80T_000140"
     CDS             63159..64031
                     /locus_tag="ACG80T_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4839829.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            64373..65245
                     /locus_tag="ACG80T_000141"
     CDS             64373..65245
                     /locus_tag="ACG80T_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS4839830.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            65242..67200
                     /gene="nagE"
                     /locus_tag="ACG80T_000142"
     CDS             65242..67200
                     /gene="nagE"
                     /locus_tag="ACG80T_000142"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFS4839831.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(67532..68383)
                     /locus_tag="ACG80T_000143"
     CDS             complement(67532..68383)
                     /locus_tag="ACG80T_000143"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4839832.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            68506..68973
                     /locus_tag="ACG80T_000144"
     CDS             68506..68973
                     /locus_tag="ACG80T_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFS4839833.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(69236..69718)
                     /locus_tag="ACG80T_000145"
     CDS             complement(69236..69718)
                     /locus_tag="ACG80T_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS4839834.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(69818..70681)
                     /locus_tag="ACG80T_000146"
     CDS             complement(69818..70681)
                     /locus_tag="ACG80T_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS4839835.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT        20012 a        15290 c        12713 g        23151 t
ORIGIN      
        1 ggaaaaatga ataatacaaa tataatcatg ttcaacagca gtgtaggtcc taaaaacctt
       61 gtaataaaat acgtgttatt tactactgct aatttaaaaa tcaactggac caccatcgta
      121 aacaattcat aacccgtgac aaagatgatc atactgaaaa attcagtaaa gatgttgaca
      181 tggataacgt ctttcattac atacatcaac catacaatta aagggagagc tactgcatag
      241 ataccgataa cgccaatata gtaagcgtca tagatagccc cgagaacaat cgtggtaatc
      301 agtaaataac gtttttcaaa cgccatactg caacataata atgccaatat cagaaagtgg
      361 gcatttgaca tataggtgcc tttcgaccac tcccctagca ttcttgttaa atgaccatct
      421 atcaacatca aaaagaacaa aacgattggc agatagtact tcattgtttc tttcttcaac
      481 atggactaat cctccgctag ccgttggaca atcgtcacaa ctgacacgtc atacatttct
      541 gcataaggtt tcacataaac ttcccgatcc agcccataac tatctggttt cgttttaata
      601 accgtaccga tcggcaagtt agctggagaa ttccctccaa gaccggatgt ctggacaaca
      661 tccccttctt tgatatccgt atcaccagtt aattgggtca ccactaaggc atggagcttt
      721 tcatcatagt ttttaagcaa accaaacgct tcgccattag ccgaagatac ccgtactgga
      781 aaatgattgg agctttcatt agaggatgac agtaattcga ttttagacga agccgtattg
      841 acctcgatca ctcggccaat caatcctttt tgcgacatga ctgccatatt tgcttcgata
      901 ccgtctttag aacccttgtc tacgatcagc atgtcctgcc aactgtcagg cgaacgagta
      961 atgacatttg ccgttacttt ttcgtaacta gtcagcgtct gattcaactc aagttctttt
     1021 ttcaacgctt cgatttctct ctcgtaattc ttgttttgct gtgaaacgtc atcatagcta
     1081 tcgatttttt cttttagccg ctcattttcg gcataagttg aaaaaagatt atggacagaa
     1141 tctactccat ttcgtaccca ccgtactggg gcagagattg ttttatcaac cattgcaaca
     1201 ctgtcattaa cgatggattg gaagaaattg ggctttcctt cattcgctcg ctgcgcagcg
     1261 gtcacactta gtatcgtcac cacgatgata acaataacca aggtaataat aatattttta
     1321 ttaggattga acttcttcac aaagacacct cgaattaatt agactcttgt gttcaatttt
     1381 atcactaaat gccgtgaaac aaaaggtaag ttgctcctct taagcttttt tttagcaaat
     1441 aatagattct gtcattttta gcctgaaaga caaatttttt tatttgttta tggatcaatc
     1501 tttgctttga ataactgcaa tcataccatc tgaaaaagaa aattctgcgg tatctgaaag
     1561 aaattcgtta cttgcataag atgtcggata ttctacttct ttattttcca atacatactt
     1621 gctgcctttt aatgtaagat tcgagacggg agtcaagcag caataagcta aatacttcat
     1681 atctggtatc tgtcttacta tatgctctcc aggcaaataa taagacaaat aattttgaca
     1741 atctctgata ataagctgat gggcaaaggg tttgaagcga ggttccatac ctaaccagag
     1801 attggctaaa agatgatcca atcttccgcc cgtagctcca atcaaagtga cttccgcttg
     1861 cgggtaacgt tcaaatgttt ttaagatacc taactgtgta tctgtatcgt ctttttctgc
     1921 aggggaacgg gtatactctt tggcttttgc aaatacttcc ttctgctctt cgttcgacaa
     1981 tgaatcaaaa tcacccactg ccagatctac tgggtagcct gcttgaagca ggtacagcgc
     2041 accacggtct atcccgacat aggcatcgta tttggacagc agagactgcg gccagttttg
     2101 cggattacct ccagcagcta ataaaatttt catttagtct aatgcctctt ttaatcgttc
     2161 gatttgtccg actggatttt cggctccata gatatatgat cctgcaacga agacatccgc
     2221 ccctgcatct ttacagattt ttgctgtttc tggaacgatt ccgccatcta cttcgattgc
     2281 ataagtccaa ttgttctctc tacgtagatc agcgagttcc gtaatttttt ctactgtttc
     2341 ttcaataaag ctttgtccgc cgaacccagg attgacagtc atgactagta cttgatcagc
     2401 caaaggcaaa acatgtttga tcatggagac tggtgttcct ggattgataa ctactccagc
     2461 tttaactcct aagctttttg tcatttgaag tgcccgatga atatgaggag ttgcttcgac
     2521 gtggacagta atgatatctg ctccagcttt tgcaaatgct ggaatgtagt tttctggatc
     2581 aacgatcatc agatggacgt ctaacggtaa tttagtaaca ggacggattg cctgaacaat
     2641 atttggccct aatgtgatat ttgggacaaa tcggccatcc attacatcta cgtggatgta
     2701 atctgcgcct cctttttcca ctaactcaat gtctctttgt aaattggcaa aatctgcact
     2761 caaaatggat ggtgctatct tcattcttgt ttcctcctat tttcgtttac cttctttttt
     2821 tgcatacatt ggttttcttt tttcgatttc ttgtaagaat tgcaaataat tgtcgtaacg
     2881 tgtctgtgcg ataccacctg tttctactct atgttttact tcgcagccag gttctttagc
     2941 atgcatgcac tctctaaatt tgcaatggac agaagcttct acaaattcag gaaattgtct
     3001 cggcagttct cctgcttcaa ttgtcaaaaa gtcaatcgag ctaaatcctg gtgtatctgc
     3061 tactaacccg ccatagatag gaatcagttc cacatgacgt gttgtatgcc gtcctcttcc
     3121 aagtgcatca gaaatctctc ctgttgctaa attcagttcc ggtgatattt gattcagtag
     3181 cgtcgatttt cctgctccag actgacccat aaagaccgat agccgttctg ggaagtaacg
     3241 gatcagttct gtcgtatcac ccgtatgatt gggtacaatc accggatagc ctattttagt
     3301 ataggtttgc ttgatctctt cgattttttc tgggtcgtct gacaagtcgg tctttgtcaa
     3361 aaaaataatc ggttcgatcg actcgtattc caaggtaact agaaagcggt caagtagatt
     3421 ataagaaaat gcaggctcta cgatacttgt gacaatgatt ccaagatcaa catttgtgac
     3481 tggtgggcga actaattgat tttttctcgg caagatttcc agaagatagc cgtctgtttg
     3541 attttcactc tcaaagatca cctgatctcc gacaagcggc gtgattttcc gattccggaa
     3601 atttcctctt cctcttgttt gataggtctc tccgtttgcg tatacatagt aaaaaccgct
     3661 gattgctttt ctgatttgtc ctttgataag tgccacctcc gtttttgttt attttaccat
     3721 acgaagagga aagtaggtgt tattccatgc aaacttctca aagtatatgg cttgaaaatt
     3781 gatcaaaaga gatagaaaca caaaaaagag aatgcaacaa atcaatgtcg cattcccttc
     3841 tgatatattt tattcagcac ttattttgaa ctttctgagg tgctagctga tgaacttgtg
     3901 gagctggaag attcttcttt tgaagagttg cttgtgctac tggagctact tgagctgcta
     3961 gaactgtctg aagagtcttt ttcagaacta gaagacgctg gatctggtcc tttagagtaa
     4021 attacgttca caaccattcc tttggtaatt gtcgttcctg cccctggtac tgtacgaatc
     4081 actttatctt tatccaccgt gtttgaatat tcggaagtct catgcccgat atattctgca
     4141 ccgacactag ctaaataact ttgtgcttcg ctttttgtat agccggagat atcattcaat
     4201 ttaggctctg ttccttggct tacgacaaaa ttgatgtaag tttcttctgc tacaacttca
     4261 cccgaggctg gatcctgact gatgatcgaa cctgatggta cagtgtcgct atagtcataa
     4321 gtagcactaa tattattgat attgaatcct tgtgtaacaa gccaattacg aacagtttcg
     4381 tagttttctc ctacatgact actcaaagtc actgtttctg gtcctttgct gactgtgaaa
     4441 gtaatcgtgt ctgttttagg atcgacttct gtatcttctg atggagattg cgaaatgatc
     4501 attccgcttt ctacctctga actgaattcc tcttcttttt gaatcaagtc agcagaaaac
     4561 cctaattctt tcaaacttgc ttcggcatct tcataacttt taccactgta atcatctaat
     4621 ttgatttttt tcgtgccgct actaatatac agagtgactt cgcgtttttg cttgactgtc
     4681 gttcccgctg ccgggtctgt cttcactaca ttaccttctt cgattttgtc atctgcaatt
     4741 tcttctgttt ttgttgctac ttctaatttc gcttttgcga gtgtttctct agcagaagct
     4801 tcagaaagat cagaaacatc aggaacaaca acttctcctc gcccgccaga agcaaagaat
     4861 acagctgttc cgattcctaa aacagcaaga atagctaata ggatcagcca ccattttttc
     4921 tgtcctttct ttttaggatc aggcagaact tcttcctcct ctggtaaggt ttcgttttct
     4981 ggcttgtctt ttggcagagg catagatttg aatgaatcag gcattggtgc atcttccgta
     5041 aggggtgtga taacttttgt ttcattatcc atcacatgag gctgccattt ttcttcgttt
     5101 gcacgagctg gtgataatac tgtagaaaga tcatcagaca tttcctcggc tgacttataa
     5161 cggtctgcag gttctttagc cgttgcccgt aaaatcacgt tttccagtgc ttgtggcaca
     5221 tttggatcaa gtgcttttat agatggaaga tcatcttgaa aatgttttaa agcaattgtt
     5281 acagcagatt caccatcaaa cggtacgcta cccgtcaaca tctcataaag gatgatccct
     5341 aaagcataaa tatctgactg cttcgttgcc atacttcctc tggcctgctc aggggacaga
     5401 taatgaacag atcctagcat cgtattcgtc tgcgtgatcg atgtttcaga taaagcgata
     5461 gcaatcccga aatccgtgat ttttacaaca ccttcattat cgatcagtac gttttggggt
     5521 ttcaaatctc ggtggatgat ttgatggtta tgagctaaag aaatagcaga aaggatctgc
     5581 tgcataatat ttactgctgt ttcataagga acaggataat gtgtttggat atatcgtttc
     5641 aagtccattc ctttgacgta ttccatcacc agatactgca tgttgtcttc ttcaccaaca
     5701 tcgtaaacac taacaatgtt tggatgaacg agttctgttg ctgctaaagc ttctctctga
     5761 aatcgtcgaa tcgcagtctg gtcattttga aagtcaaatc gtaagacttt aactgctaca
     5821 tcacgatcca agattaaatc gtgcgccaaa aatacatttg ccataccacc gctaccgata
     5881 tttccagtga tttgataacg gccatttaat tttttgccca actcgatcat acactgtcct
     5941 ccttatattc gataagaaga acggtgatat tatccgctcc gcctgcttca ttggcacgat
     6001 caatcagctc agttacttta tgagacaatg ggccttcttg attgatgatc gttcctattt
     6061 cttcttcaga cagcatgttc gtcaagccat ctgaacataa cagcagtcga tcttttagtt
     6121 gccaaagatg agtagaaaca tctacttcga ctgttccagg catgccgaca gaacgtgtta
     6181 aaatattttt ccttggatgg ttcactgcca tttcttcgct gatttctcct gatttgacta
     6241 gttcattgac taaagaatga tcctccgtca attgcatgat tttatcttca cgaatcaaat
     6301 aagctctgct atccccaaca ttggcaatcg tgaaccagtc atctgacaga gcggccgcta
     6361 cgattgttgt tcccataccg ttatattccg gctgttcttg tccacgctga taaatcagca
     6421 tattttcttc ttgaatcgat tgaatgaacc attgggcgat tttttcatcg tcaacagctt
     6481 cttgtttttc ccaagcttcg cctagattcg ttaccgccat ctgactggca atgtctccgg
     6541 cacgatgacc acccatcccg tctgctaata cagcaagctt gagacctgaa cggctggtaa
     6601 atacactggc atagtcttga tttgtgttac gtcgtcttcc tacatctgat tgatattcaa
     6661 tctgcatttt ttcacctcat tactttttgc gcaaacaaca aatgaaaaat ccatctgtgt
     6721 aaaactgatg cggatacaaa gtcaacattt tctcttcgat tgatgattgt agcataggat
     6781 tggtcaaaac atcaatcctc tcaaaatcag ggtgtctttc caagaaagca gaaacaacgt
     6841 cttggttttc ttcttttaag atcgtacaag tgctataagt cagaataccg gaagacttta
     6901 aagttggtgc gcagctttct aaaatttcca attggatttt tggtaaattc gctaattcat
     6961 ccggctgttt tttataacga atatctggtt ttcgccgaag cagcccaaga cctgaacaag
     7021 gcgcgtctac taaaatccga tcaaactgct ctgcttcgaa ctgtttactg acttctctag
     7081 catccatctt ttgagcgtag acagcagaag agacacctag acgtgctgca ttatcttcaa
     7141 tgagtttgac tttgtgttcg tgtacatcta aagcgaccac ttctccaccg catgacggat
     7201 ctaaaaaaga agcaatatgc gtcgtcttac ctccgggtgc tgcacaggca tctaagacat
     7261 gatgttcagg ctcgatttgc atagacggcg ctacaagcat cgaactttca tcttggatcg
     7321 tcatgacgcc atcgtggaac aatgagctag aagctaaatg cccttttttc gctactatac
     7381 cgtatggact gacttggctt ttttcagctg tgatctcttc ttcttgcaaa acagtgatgg
     7441 cctgatctct ggaaagtcgg cgtgtatcta ctcgggcact agcatggcta ggctgatata
     7501 gcgataaacc gagcttttca gtttcttcgt atccgatttg atccaccagt cgtttcgtca
     7561 tccatagagg caggctgatt ttgacagaaa gtctttcgat aggatcagca atgccttgca
     7621 gatctggtgc tccttgacgc tgataattcc tcaagacacc atttacaaat ttccctgtcc
     7681 caggatttcc ttttactttt gcgatttcga ccgcctcgtt taaaatagca tgtgcaggga
     7741 ctttatctaa aaattctaat tggtatacag aaagataaag aagcatcttg acccagtcat
     7801 ccactttttt tgctttagcg atcaatggtg caacgtaaaa ttccaatagt cgctgatggc
     7861 taatcgtacc atagatcaat tctgtaaata aacccacatc tttttgattt agctcgcttt
     7921 gtttgatcat ctcattcaac aaaagattcg agtaggcgcc acccttgttc actcgctcta
     7981 atgtcagtaa tgccatatat cgtactgatt ttttcatttt tttagggatt tttttcatta
     8041 tttcacctgc tgaccaatct gaacttgctg accgcttccg ttaaggaaat catgtacggc
     8101 ctgttttcct ttaccagctg gctgtaattc attgatggct aagacggtct gtttaccgca
     8161 agcaatccat agattttttt tgtctttccg gatgatcgta ccaggtgctt tgtctgtagt
     8221 ttctgccaat acttcaaccg ataataattt ccagcgtatg ccctcatagg tcgtgaatgc
     8281 aattggccaa ggacgcattc cgcgtacttg gttgtcaatt tcttctgcag ttttatgcca
     8341 atcgattgct tcttgttccc gtgtgatgtt tggcgaaaaa gttgcttctg actcgtcttg
     8401 aggtactggt ttttgtccat ccaaaatatt gggcaatgtt tctagcaata gctttcttcc
     8461 taaaaggcta agcttttcaa acatcgtccc tacatcatct tgtttagtga ttggtatgct
     8521 ttcttgcgca tagattccgc ctgcatccat ttttttgatc atttccatga tcgttacacc
     8581 agtttctttc tccccattca tgatggcata atgaacaggc gcacctcccc gatatttagg
     8641 aagtaaagaa gcatggacat tgattgcccc taattttggt acttgcaata acttttcagg
     8701 caagaattgg ccaaatgccg cagtgacgat cacatcaggc accaattcaa tgatttcttc
     8761 catttctggc gaaccggaga ttttctctgg ctgcagcaca cggataccgt gcttcagagc
     8821 cgcttttttt accggtgtcg gcgtaattac tcgtttccgc ccaactggac ggtcgggctg
     8881 tgttaccact gcctggatct cgtaacctga ttctatcagc ccttcaagaa tcggcacaga
     8941 aaaagcaggt gtgcccataa atactagctt agtcatcaag atgttcctcc atatattctt
     9001 caagttcttc ttccgggatc tgctgtatca ttttctcaat gaacaagata ccgtctaagt
     9061 gatcgatttc atgctggaag gcacgggcta aatagccaaa agccgtcact tctatctctt
     9121 ctccgtctcg atcataataa cgcacggtca cttcatctgc tcgcttgacc gttccataga
     9181 catgcggaat actgagacaa ccttctacgt caaggctttc accttttgct tcgatgattt
     9241 ctgggttaat cagttcaaat ttctcgcctt catctacttc gatcacagca attctcttgt
     9301 tttgtcctac ttgaggagca gcgatcccta ttccgtcatt cgcaatcatt gtctcatata
     9361 aattgtctaa taaagcgatt gtctcgtccg tgattacatc gattggctga gcagttcttt
     9421 ttaatttatc attagggtgg atcaatatag gatagcgcat aatttggccc tttcttaaat
     9481 aaagtttaat ggttcgttgt caattgccac aaaaagacct tgtcgctgtt ctttctggct
     9541 tccattcaaa atttcatcca aaacatgagg aagattcggt tcatgtttat atttgatgat
     9601 tagttgataa taatatcggt ttttcactct caaaatcatg ctgggcgttg gccctaatag
     9661 caagctttga gggcttaaca cttcttttag ctgattggca atctgaaaga ttttctttgc
     9721 cgctacttgt tcttcaggat ggctggcggt tattttcaca gtaaagtaat aaggaggata
     9781 gcctccacga tgccgtacat gcatttcctg ctgataaaac tgctcataat tttgtttttt
     9841 agccaattct atggcgtaat gttgaggatt gaatgtttgg atcacgactt cgcctgcttt
     9901 ttccgctcgc cctgctcgtc ctgaaacttg ggtaagcaat tggaatgtat gctcactaga
     9961 acgaaaatcc ggtaaattca gggaggtatc agcattcaac accccaacta atgtcacttc
    10021 tggaaagtcc aaacctttag caatcatttg tgtccctaat aatatatctg cttcttttgc
    10081 accgaatttt tgtaaaatct gttcgtgtgc cccttttctt cgagtggtat ctacatccat
    10141 acgcaaaatc ctagcttgcg gataaagttc tcggagttcc tcttctactt tttgagtacc
    10201 tgttccatag tagcggattt tattgccgcc acaattagga cagcgattgg gaataggttc
    10261 ttcatgtccg caataatggc agcgcattga acgtgtatcc atatgcagtg taagagagat
    10321 atcgcaatta ggacatggaa gaacaaatcc acagtcccta cacatcacaa aagacgaata
    10381 tcctcgacga ttgagcagta ggacagtttg ttcttttttg tctaatcgat tttgtatttt
    10441 ttcctgcaaa ttagcagaaa aagtagaggt ccgatgattc tgaaactctt cacgcatatc
    10501 tacgatctca atagctggaa gctgtgcagc tgctttggct cggtggttta actgcaataa
    10561 ctgataaacg cctttttgag ctcgtgctct tgattctaaa gagggcgtcg cgcttcctag
    10621 aactattggg caatgatgat atttactgcg ccaaattgct agatctctcg catgataacg
    10681 cggtgtctca tcttgtttgt aacttgattc atgttcttca tcgataatga tcagcccgat
    10741 atttttcagc ggggcaaaaa tagcagatcg tgcgcctact actacttgcg cttcttcccg
    10801 ctcaatcttg cgccattcat catatttttc accttgggaa agtccgctat gtaagaccgc
    10861 aactgattct ccgaagcgtc ctttgaatcg ctcgaccatt tgcggagtca atgcgatttc
    10921 aggcactaac ataatagccg ttttattttc tgacaaaaca tcagcgattg cctgaagata
    10981 tacttctgtt ttcccgctgc cagtgattcc ctccagcaaa aagacctgat ctttttgctg
    11041 ctgacctgct gtgatgattt gttcgacagc atttttttgt tctgcattca gttccaatgc
    11101 cgtcgtttgg tcaaatactc ggtctttata agggtcgcga taacgttctg attctataaa
    11161 ctccagccag cccttttctt ttccttgatt caagacagca gtactgattc ctttattttt
    11221 gaaataagca attggttctt cttggctcag ttcagataaa cacaaaagaa gttctttttg
    11281 tttcttggca ttttgccgta attttgccca ttcttcttcg atttgctttt cagttaacaa
    11341 agaacggacc aaacgaatcg tttttacttt atttcgtgta tggacttcat atttgatatc
    11401 tactaattgc tcttggcgcc agcgaatcaa ttgagaaagg ctgccttctt cttctgcttc
    11461 cttccaggaa atcacttctc gctgatcaaa caatacctga acttctggtg tgtctgccaa
    11521 aggatagatc aatttatcat attctgcttt catcacactc ggaagcatcg tttgtagaca
    11581 agtgattttg aatgcgtaag taattttttt catataatca gctaatgaaa gtaattcttc
    11641 attgaccacc ggagcaaggt ctagcaaacg aataactgcc ttcaaagatg gattagctga
    11701 ttcttctgat ttttggatag ccataacaaa tccttggaca tgacgattgc cattaccaaa
    11761 agggacttct actcgcatcc cgacttgcaa agctgtttcc atttcagagg gaaccaaata
    11821 agtgaatggc tgatcggtct gcatagtagg tacgtccaca atgacttcag ctgttaatgt
    11881 catggttcgt ttcctccttt ttttgtcttt tcttatcaag cgacaaaaag ttccttgcct
    11941 attttactag agatcgatgg atttgtctta tcttctgcca aatcctaaca aaaaattaac
    12001 agcagataag aaaaaaaaga actgtgtcaa aaagttcaga cacagccttt tctaccagac
    12061 aataagtggc gaatgtttga aaaggaaaca gctgggtttg cttttctttt tagatgttcg
    12121 ctctattttt ctttattaca ttttttgctc ttcacggata cgtgattcca attcgtgttg
    12181 ttcacgctct ttttgtgctt gacgttcctc ttcttccatc ttcaaacgtt cacgttttaa
    12241 ttcaggatgt ggatcattga ctacatttcc tgcatcgatt tcttctaaag cttgaccgac
    12301 gctttttact gaatcaaaag aatctagagt aggctgtgca cttgcatcca attcatgcgc
    12361 gcgtttactt gctaaaatca ctagagaata ttttgaattt acacgatcta atagtgagtc
    12421 aattgaaggt tttaacatca taagactaca tctcctttaa cattttaata tatttaccga
    12481 ttacgcgatc tactcgaaaa tgttcactgg caataatgtt tttgattcgt tcaactgcta
    12541 gcggcacttc atcatttaca acagcatagt cgtaaagagc catcatttcg atttcttctt
    12601 ttgcaacttt cattcgtttt tcgattactt catctgcatc tgttcctcgg cctacgatcc
    12661 gtgattttaa ttccgctaaa tctggcggag tcaaaaagat gaatacacca tcgggcactt
    12721 tttctttgac ttgttttgct ccttggactt caatctccaa gaaaacatct tttccttcat
    12781 ccaatgtctt gttgacatat gacaaaggag ttccataata attcccgaca tattctgcgt
    12841 attccagcat ctctccagct tcgatcattg attcaaactc ttctttgctt cggaagaaat
    12901 aatcaacgcc ttcgacctcc ccttcacgca ttttacgggt agtcattgag atcgaatact
    12961 gaaaatcatt atcctcactc tcaaaaattg cttttcgtac tgtgcctttt cctacaccgg
    13021 atggaccaga caacacgatt aataatccac gctctgacat gacgacgtcc tttcaactag
    13081 tctttttgta cttatcgttt attttgcact ttttttctgc aactttcaag gttatcttta
    13141 attatagcta gaaattggta aaaaagctac cctgtaccaa gaggtcgtaa aaaagctgtc
    13201 ctgcatcaag taataagaac agccaaacat aaacagcttc tcatgttttt cgtttggttg
    13261 gggttattat cgcagatgca agcttttgaa cgccgtgtat aagaggtcgt gaagaagccg
    13321 attagtttcg agtatgaaga aaaattttgt gaaaatagtt tctcacattt ttgcataaat
    13381 ttggcttaat accgagaaaa tggtttttga acatcactta ttcaaaataa aaaaagattg
    13441 tgacacgaat tttgtcacaa cctttaaatg tattcctaga gcaaagaagc taagcgaaga
    13501 aatccaattc tgtttattta ttgtatttct ttgttcagca tccttgctaa aagcccatat
    13561 tggacaaagt attcactaca cccatgatct catcttttcg agaacgtgct ttttctgtcg
    13621 gatcttctac ctcttctgtg tcaattcgaa caataaattt ccgattcatc gtcacgtaag
    13681 caccgtcgtt gatcaaaata aacagttcgt tctcattata gtctgcacag gcatctttcc
    13741 atacgtcaaa gccgtttttt tcagaaggga tatcccgttt aaccacagtt tttccactta
    13801 attcgtggaa cgttacgtca taatatttca ttatctctac ctctttcgtc attcaatatt
    13861 ttgtacttgt tctctgattt tttctaagat cgtcttagac tggacgactt gatttttaat
    13921 ttcgattgca tttgatttgg aaccgatcgt gttgatttcc cgattcattt cttggatcag
    13981 gaaatctaat tcccgaccta caggacattg tgcttcgata agttcatcta gtttttgtac
    14041 atggatcgcc agacgatcca gttcctcgtg aatatctccg cgttccaaca agatggctag
    14101 ctccgtcaac aaccgttgct catcgacttt cgctccaaga aattcctcca gctttttttg
    14161 ataccttatt tgaaattcct tttcatagat ttctacaaaa ctatcgagtt tttttagtac
    14221 ttctttgaat tcttcagtat tctttttcaa cactaaagct aaagcttgac cttctttttc
    14281 tcgactcgcc tcaatagaat caagtgcgct tttcaatgta tctagagcta actgtgccaa
    14341 ttcttccatg ctctcttccg gtttttcttc taccgttacg aagttttcgt ccagcacgaa
    14401 gcgttccgcc atccgactaa ttgacacttg attttgacta ccgtatcgtt gttgaattcc
    14461 gtctgtcaat tgagtgataa gtgaatcaat caattcccag ttgatgacga tctgtttttc
    14521 gtttttgcca atataagcta gattcacaaa tacttcgatt cgaccgcggc ttaactgctg
    14581 tttaataagc tgacgaaaat cgttttcaac aaagttcagg attttaggac ttcgtatttg
    14641 aacatctaga aaacgctggt tgacactttt tatttcaata tctaactgat acgcttttgt
    14701 ttcgcggatg gctttgccaa aaccagtcat acttttcatt cgacagggtc cttcctcatt
    14761 acatattttc tattgctttt tgcagttgtt caaactcttc gttcgtcaat gtcaggcctt
    14821 tccccatttt ttcatggtct ggcgcccaat ctcgaagatc gaattttggc gggcgaccat
    14881 tccagctgac taggttcagt tctttacgcc accccttgtt gttttctgac aatatagcga
    14941 tttcttctac gatttcatat gaaaattctt gtgccatggt tgttcctcct tgagataata
    15001 taaatatagg atcagtaatg tgtcaactgg ttgcttaagc aattcatgct gcaaacgatc
    15061 cttgcgtttt ccttgtagca gataatcaaa ataaacagtt ttcgtgcgct cattttgtat
    15121 tttttgctgg tattttcgat accttcgcca ttcttcagta cttaattgac caatttgtgc
    15181 caataaatca tccggcatac tttttatatc ttgttggcag atcatttgaa tcatcatgtc
    15241 tacttgatag atttcattgg gcagtaaaaa atgttttttt gctaaaagtt ctagcatcgc
    15301 caatccgcat gtctctggta cgaccctgtt cttatataaa ttacgcagtt tttgccaaaa
    15361 ttttatgctt ttgaaaaaag ataattctaa ctgaatttct tttgttaagt aagacaaaaa
    15421 tcctcgccag tatgttcttg caggtttaac agaatgaaat gtataccagt tcccatagta
    15481 atagcctaat cgttttttca tttccagata gcctaattgt aaatttaatt ggttcctttg
    15541 gctatcaaaa agcagaaagc tgcctagtgt ccacaatgtt tgacatttcc aatggataaa
    15601 aatgtctggc atacgaattt ttttcgctgg gcctggccct tgcacatcta cgacaaatgc
    15661 ctctgttgct cctttgttaa tcgcaatatc aataggtatt ttgttgcgat acccaccgtc
    15721 tgcatattta ttttttccga tctttcgata agccatagcg ggataaaatg aagcagaggc
    15781 gagtatccaa tctggtattt cttctgtttt tgtctgttgg atatcagtca ctacttccgc
    15841 tagttctggc agtttagtag acaccgtata taaacgtatt ttgggattat gtttgagtaa
    15901 ggacaaatcc aaattgtctt tgaccatctt ttccaatgga gcagaagaaa ccccgccttc
    15961 aattagtgct gttttcgtca tttggcgtct ttctcgatag aagcgttcac gaaggtcttc
    16021 aactgcagcc atttctggta acgcaagcac ttgtcttgtc gacagctttt tccataaatt
    16081 cacagctttt ttcatatctc cttgtaaaat caatgcgccg ttcaacgcac ctaccgaggt
    16141 acctgtgata atctcaaagt tgatatcatg ttccttcagc gcctgccaca caccaatctg
    16201 ataagcaccg tgcgctcctc ctccacctaa aacaagtgct gtatgataga ccagcttttt
    16261 atttaattgt gtggttcctt gatagccgtt tttatgaaaa atacgcaacc attcttctga
    16321 tagaggagat tgaaataaaa aacaacagtt ttctttaata aaaaaacgag cgcaagcttc
    16381 catataccga aaaaattttt tccattcaaa agtttcacct tcaatcaaca gattattaaa
    16441 aataacatct cctctacttt tagcaatagt aaaatagacg gtatattttt tcgatactgc
    16501 tttaaaagca cattgctttt ttgcagaacg ccaaaaagcg tctctacttt cttgatgact
    16561 ttgaaaaaat ggaaggggtt gttttgcttg ctggataggc gaatacagcg acattttctg
    16621 tgaaaagtaa acttttttta tctccatcgc gctctccttt ttcttcaagt ataccagtac
    16681 gtcataaaaa ggtcatgagt ctcgtattcg actttgtaga ttccataaga aaacaattca
    16741 taaaacctcc tttaaataaa aaaatacaaa aatcacaaaa attaattgaa aatgagagtg
    16801 ttttcaatga aaacgtgatt ttgtaatgga attttaaaat aaaagaaaca agctttttta
    16861 atgcaaatgt tgtaaaataa aagagattaa gaggaaataa gaggaggaac atgatgactg
    16921 agtcagttta tgtacatatt gataccacaa gtaatgctgt tttaacaaaa gggctgactg
    16981 ctactgactt tgcaaacagt atcgtgcatt ttcctcaaaa cttgctcttg cttgatccat
    17041 ctgcttctgc tggagaatat gaatcccata cagggctgaa agtaatccgt ggtacagaaa
    17101 atattcagcg tttcttttcc tcttctcgta acaggcgtgg attcgatgaa ttgaagtgga
    17161 tcgactttac tgatttaacg atgctgaaag aattgacacc tttagaaatt tcagaattat
    17221 tatattttgg tcatatgaaa acacatcttc attcgccgtt tttttataaa ctacagaata
    17281 attttgtata cttcgatctt aatgatcagc tgaatcggat ttattatcgc tatttagatg
    17341 aattttatag gatttttgct agtaaattaa gttcgatctt gtcagataag atcaatgtaa
    17401 aaaaatcttt tttcaaaaaa gaaacacctg ttgaaaaaat cagtgtggaa ttattgaaag
    17461 aaatgcgtaa tttgatgcag gaagggatca ttttttcttt tgatcaagtt ggtttgatcg
    17521 atggtaaata tcgtatccca atctatgtga ttgaagatta tatttggaaa ttaaaaggca
    17581 cccggtatcg agaagaggaa atcatcgcta ctttaatcta ctctgtcacc gatcgacact
    17641 ggcaattggt agaagatgcc gatctttctt atctgccatt gaagtagatc tatttcttat
    17701 caaaaaataa aaaggaaaca agaaactagg acatgattct gtctcaagtt tcttgcttct
    17761 tttttattta cagctcctgt cgtttatctg gcacaaaaat cgaaggagtc gtttgttgga
    17821 tccctaataa aacgatgatc gttacaatag ctgtactcaa cccgctggca ccattcatta
    17881 cgaaagaaaa gagccaagga ttcatccccc atagtgcata cgctccccag aaaaccactc
    17941 ctgcaataaa atgccaaaaa tatctggaaa gtgcgccaat caatgcacca aacacgatgc
    18001 tttttctaat ttgtttcgta ttatcgtcta catagccttt ttggacattt gaagccatga
    18061 gccctgctgc gcctgcaaaa gtataggcaa tcgggtactc aatcaacact tgaacgacac
    18121 ttaaataata aacttgtccc aacggaaaat gtaggatgcc ccaaatgaaa cctgccagca
    18181 tccctgcctt tgtccctctt cttaacgcaa aaagagtcaa tggaatctgc cctaatgaaa
    18241 tagaaaaact gctgccgatc tggatcggaa tcaacgacaa caccatcgct aacgctgcaa
    18301 caatcgttcc ttctacccaa actctatttt ttcccataaa aaaacttcct cctttgaata
    18361 aaaaagtcac aaaggaggaa gcagctttcg aaaatagtca aacagctgtt tattcacaat
    18421 tcctacgctc gcataaccga tacaggtacg aagggtttag aaatagattc aatctcagcc
    18481 caattggact cccctttgtg agtttattta ttatttattt tttgtttcaa acaatggact
    18541 aactggtttg ttttcatgga tacgtttgat tgcatctccc attagtccgc caacacttac
    18601 ttcatcgatt ttgtcgattt tacgatcttc tggaagatag atcgagtctg taacaactag
    18661 acgctcgata gctgaatctt ctattctttg taaagcaggg ccggataata ctggatgtgt
    18721 acatgaagca tacacactag ttgcaccagc ttctttcaaa gcattggcag ccaatgtgat
    18781 cgtacctgct gtatcgatca tatcatcgat cagtacacaa gttttgcctt tgacttgtcc
    18841 aatgatattc attacttctg caacatttgc ttttggacgg cgtttgtcta tgatcgcaat
    18901 cggagatttc aaaaattccg ctaatttacg tgcccgtgtg acaccaccat ggtctggtga
    18961 aacaacgaca acatcatcgc ctttgattcc tttttctaag aaataattgg caataagtgg
    19021 cgcacccatc aaatgatcga ctggaatatc aaagaaacct tggatttgga ctgcatgaag
    19081 gtccaaagtc agcattcttg ttgcaccagc tttttgaatc atgtttgcta ctaatttcgc
    19141 tgtgattggt tcccgtgcac gtgctttacg gtcttgacgc gcatagccat aataaggcat
    19201 gactacgttg attgtttttg cacttgctcg tttcagtgcg tcaatcatga tcagcaactc
    19261 catcaagtta tcattgactg ggctgctcgt tgattgaata acatagacat gcgatccacg
    19321 aatactttct tcaatgttta cttgaatttc tccatcacta aattgagtaa cagatgattt
    19381 tcctaactcg acgccgactg ctgctgcaat tttctctgct aatgggcgat tggaatttaa
    19441 cgcaaaaatt tttaatcttg ggtcaaagta atgttttgac atggggacct ccactttctt
    19501 tttcacaact ctagaattct acctaataaa tagtagtcat attttttaaa taaatcaagt
    19561 gattttgcaa aagtcttagt ttagataagg gagttttttt gcatatcctt ctttattgac
    19621 ttgtcttgct ctcgcaattg ccagcgcata ttctggaata ttgtctgtaa ttgttgaccc
    19681 ggcagcaatc gaactgtttt tagctacctc gacaggtccg atgatattcg tgctagaacc
    19741 aataaagctg tggtcgccga cagtcgtatg atgtttgttc tttccatcat agttgacgaa
    19801 gacaacacca catccgacat tgatttcttc tcccagcgta gcatcaccaa cataagtcaa
    19861 gtgaccgact ttagttcctt tgccgatttg agcatttttc acttccacaa agtttccgat
    19921 atggacgttt tcgccaattt cagcttttgg tcgtaaatgt gcataaggtc ccacatctgc
    19981 atgtttcttg acatggctgc tttcaatgac tgagttttca atgacgacgt gatcctcaat
    20041 cctactgtct acgatttttg aatgtgaccc gatgacacaa tcagaaccaa tgactgtatt
    20101 tccttggatt tgtacacctg cttcgatcaa tgtgtctgga ccgatttcta cgcctgcatc
    20161 gatgtatgtc gttgctgaat cgacaaagct tacgccattg accatatgca tctggttaat
    20221 tcgctttctc atgatctcat ttgctttagc taacgcgatc cgatcattga ctcccattga
    20281 ttcttcaaaa tcatccgttt gataagcagc aactgtatgg ccttcttctt ttaagatttc
    20341 gatgatatct gttagatagt attcaccttg agcattgttt gttccaactt tgcttaacgc
    20401 atcgaacaaa gcttcgttgt caaaacagta agttcctgta ttgatttctt gtacaagagc
    20461 ttcttctggt gttgcatctt tttgttccac gattttttct acgatcccaa tatggtcacg
    20521 gatgattcgt ccatatcctg ttgggttttc tgcttgtgcc gtcaagatcg ttgcacttgc
    20581 attttttcct tggtgatatt caaaaagatt gttcagcgtt tcagtagtca ataatggtgt
    20641 atcgccactg ataactaacg ttgttccttt ttttccttcc aagaaagaag cagcttgcaa
    20701 aacagcatgt cctgttccca attgttctgc ttgtaacgca tatttactgc gttctcctag
    20761 ttgtgcttta acttgttctg ctccgtgtcc aacgatcgta atcacttcat ctggtttcgt
    20821 ttcactgacc cgattaatga tatgttctac cataggttta ccagaaacgg gatgtaaaac
    20881 tttatataat tttgacttca tgcgggtacc tttccctgcc gccaaaataa ttgcataacg
    20941 tgcgtccaac tgcgtcactc ctaaatatag tttgctgatt tcagtttatc ccagtatacc
    21001 cattttttca acaaaacaag tgattttctt ttatcccagc gttttctgga taaaaaaata
    21061 gatagaccat aacgggccta tctatttggt aaccggtgct ctcctgccaa ctcctcggtc
    21121 acaagtcaca ctactcataa aacatgaaaa gctgtttttt gaaatcgttt cttgtgactt
    21181 agagttaaac ggtaggctta caacctcttc attaaacgtt tttcaatcag ctgaccttcg
    21241 gtattagctc aataaacggc aaaatatgag gagcaatttt tccgtttatt gagctaatac
    21301 tcaggacagg acgctgattt cacaacctct agataaacgg tgttcaaaag ccagtttctc
    21361 ggtattaagc caaatttatg aaaaaatgtg gaaagccatt ttcacaaaat ttttcttact
    21421 actcgaaact aagcggcttt ttcacaacct cttattctga aaaataattt cctggcacaa
    21481 ccgtaatatt tttcgtttga gtatctacat ctttgacata aagcagtgac gtataatcat
    21541 cgatggcgcg atgaccgttg aattttgatt cggcaaatac agtgatgcca acaagttctg
    21601 attcgaattc ttcaatcaag ctcttcattc cgttgactgt tccgccgcct ttcatgaaat
    21661 catccactac aaggacacgt gaaccacgtt tcaaacttct cttagacagt tccatctttt
    21721 caattctttc agaagatcca gacacatagt tgacacttac tgttgatcct tctgttattt
    21781 ttgagtcccg gcgtacaata acgaatggcg cattcagata ataggacaca gcttgggcaa
    21841 taggtacgcc ttttgtggct acagtcatta cagcatcgat tttttcacca aggtatttag
    21901 aagcaatgat tcttccaact tgacgcagca gttcaggttc tcctaacaaa tcagagagat
    21961 aaacatatcc tcctggaagc agacggtctt gttctgatag acgttctgct agagattcaa
    22021 tatatttttt cgcctcttca tatgaaattt cagggatgaa caatacacca cctgcagcac
    22081 ccgggattgt ttccaaggtt ccataccctc gttctttaaa tgttttcttg atgattccca
    22141 aatcttcact gatagaagat ttagcagatt cataacgttc ggcaaagctt gttaaggaaa
    22201 tcaattcatg cgggtgattc aataaatact gtgtcatgtc aactagacgt tcactgcgac
    22261 gtactttcac ctattccaac tccttttaag aaattttatc gttttacatt atataagttt
    22321 ttcttcaaaa attcgagcat tttatgaaaa aataagaaaa tagttcggta tttattaaaa
    22381 taaaaagaag cctataaaaa atttttcgtt tttataaggc ttcttttctt tataatttag
    22441 ttattttttc ttctgaaggc aaccaatatt cgtttgatga tatttacaat caggaacaaa
    22501 gcaataaaga ctaatgtgat tgttgctcct ggaggtgtat ctagataaaa cgagctagtt
    22561 agtcctgaca gcatccctag aaatcctaca caaacactga tagcaatcac gacattgaag
    22621 cttttgccta atcgcatacc gattgctgcg ggaagaacca tgattgcgga tatcaaaagt
    22681 gcaccggcaa tcgggatcat cacagcaata gccacaccag taatcacatt gaacatcatc
    22741 gacatcaaat gaacgggtag cccatccacg tgggcggtat cttcatcaaa agtcagaaca
    22801 tacatcggcc gtttgaataa aaagaacaat ataccgatgg caacgaataa gattcctaaa
    22861 aagatgactt gctcccatgt aatggtcacg atcgaaccaa acagatagga ttggatactt
    22921 gttgctgaat tccctccgct caaattcatc aatactaacg ctagcgccaa accaccagcc
    22981 attaaaatgg caatggagat ttctgaatag gttcggtaga tcgttcgcag atactccaat
    23041 atcacagctg ctaatacaac gactagcatg gtcgtaagtg taggattaag gttcaaaaag
    23101 aatcctaacg ctacccctgc taaagagaca tgagaaagcg tatcagccat cagcgattgt
    23161 cttctgatca ctaaaaacac acctagcatc ggggcgattc cggcaatgaa gatagcggca
    23221 agaaacgctc ttctcatgaa ttcatatgaa agcaacgcca ttgagaatcc tcctttctca
    23281 ccagtcggat ctgtctgtcc gcatactgtt tgatatcttc atgatcatgg gtgatcatta
    23341 gaactgattt cccatggaca tgcgcactat gtcggagaag acgataaaat tcatttctgg
    23401 attcttcatc catacctgtt gttggttcat caagaacaaa taagtcaggg tcagtagcaa
    23461 aaatacgcgc taaactgatt cgctgctttt gtccgccaga caattcacct attcgccgat
    23521 ttcgcatatc ccacatccct acagcttcta aagcacgctg tacatgttga tgatcttttt
    23581 cagagagacg cttgaaccag cggcctcttg gatatcttcc tgaacggact aattcgatca
    23641 ccgtactagg aaaacctgca ttgaaagatg caacttgttg aggaatataa ccgatactta
    23701 atttttttcc ttcttgattt ttctcagcaa tctttatcat tccttttgaa ggtttcagta
    23761 atcctaacga tgctttcacc aaggttgact tggctgctcc attttctcca gttaaaatga
    23821 caaattcacc cgcatctaca tgataagaga catcttctag tacaggttca tcatcatagt
    23881 aaaaggtcaa atcagatatt tcgatataac gcaccttaca acctccattc taaaacttaa
    23941 ttgatacttt cttttaaagc tttcagattt ttttccatga cagaaatata attttcgcct
    24001 gctttgattt gttcattagt caagctttct aaaggattca acacttctag cttcactcct
    24061 gtttcattcg acaatgtctc agctaccttc gaagaggcat tttcttcaaa ataaatcact
    24121 ttgatgttgt ttttcttcac aaattctttt agctcagcta agcgacttgg tgtgggttcc
    24181 tgatctggtg aaacgcccga aatcgctact tgttttaagt cgtattgatt cgctaaataa
    24241 gcaaaagcag catgctgagt gacaaatgtg cggtttgttg catcttttag ctcatcggag
    24301 tatttttgat ccagtgcttc aagctttttg atataagcat cggcattctt cgtaaaagtt
    24361 tcctttttat ctggatattt ttcgcctaat tcttcagcaa tcgttctgac ttcttttata
    24421 gctaataccg gatcgagcca aacatgtgga tcgtattcat gtgaatgacc atgcccttct
    24481 tctgattctt catgttcgtc tgcttcttgc gattctaaac ggtcgattcc tttagctgct
    24541 tcgatcatca aggtctgttt gctatccact gaatccttta acgaatccac aaaaaattcc
    24601 atgtctgaac tattatacac gaaaacatct gcatcggaaa tttctgctct ttcttttgca
    24661 cttggttcga aatcatgcgg ctccgttcca gcagggatca aaagtttcac gtttccttca
    24721 tcccctacga tctcttttgt aaaatcatac atcggataaa aagtcgtaac gatcgtcaaa
    24781 tcctcttttt tgtctgcttc cttgttcgta tttccacatg ctgcaaacag taaagcacct
    24841 atcattaccg tagctgtcaa aagatatttt ttcatttata tgctcctttc aatgaacaaa
    24901 tcgtaatttt acgatttatt aacgtgaact aatgtatcaa aataaaaaaa cacagtcaat
    24961 agaaatccat caatttctga tttaagcatg tatattcttg attaaatcgt aaaatggatg
    25021 agttgctgct catatctagc catttcacta caataaaaaa ctgaagaata ccattttagg
    25081 attcctcagt ttttcatcac ctattttact tcttatattt tattgcaggg ttcgtactaa
    25141 ataaacttcc tcacaaaaac cttttaaccc attatatact ctctgcgctc ttgaataatg
    25201 ttgacacaag gcaaaaacgg ttggtccgct tccactcatc aatgcagcat ctgctccgta
    25261 cttgatcata cgctctttga tttgatggat gactggatgc cgctttcccg ttatatcttc
    25321 taaactgttt cccatttttt tgatcatctg ctgataatct tgctctcgga tggcttcagc
    25381 aagtccgtgg atgtcttgat gttggatggc ttgcgtatct acttctctaa acactgtacg
    25441 tgtagagaca ctcattcttg gtttgaccaa cacaacccag cactgtggca tagaaggtaa
    25501 tacttggatt tcttcccctt tgccaccgac aaatgccgta cttccgtaga cacagtaagg
    25561 aacatctgta ccgacttcca aaccgatttc tgccagttct tctaacgaac agcccagatt
    25621 ccataattgg ttgatccctc ttagagcagc tgcacagtct gtacttcctc cgcctaatcc
    25681 agctgctact ggaatattct tcttaacaaa gatcttgaca ccggttttga tattgtattt
    25741 attttttagc aataaagcag cttgataaac attgttccgt tcatctaccg gtaaaaatgc
    25801 tttatttgtt tctacaatga ttttgtcttc tgttatttct tcaaaaaata gatgatctgc
    25861 caagtctacg ctcgacatca ccatttccaa ctcatgatac ccatcttgcc gtctatataa
    25921 tacatccagt cctaaattta tcttggctgg cgctttttca atcaattcca tcctctcacc
    25981 cattcgaaaa gtctaagtcg caagtattct atcataggga aaaataaaag tatagcttat
    26041 gtctgcctag tggttgattt tgtcatcgtt tcaacagctt tcacagaatt ttctcttcat
    26101 aatccaaaca agttctagtg attcttataa ttctgtgagc acttcttctg ccaaactcgg
    26161 aacatttcct ttttcttttc ctatcagact gagtagctgc atggtagcaa atccgaaatt
    26221 acttcctttc ctataagcaa aataacgtcc ttcccaatga tcgacatatt ttaatttctt
    26281 ttcataagca gactggaagg aatagaaaaa attaccataa ttatatacga tatgcatgac
    26341 tttttcttga aagaaagaaa aatctgtttc tccaacatta gctaatggag ctaagcctag
    26401 attggcttcc gagtagccca ttttctcgta tacaataaac agattggcta acagatagtc
    26461 agctaattcc tcagattctt taggtaagac ccgcaacaaa tcatatcccg cctgcttctt
    26521 cgtcatcggc tgagcggtga tgaagccaac gactttttga tctttacgga caataccgac
    26581 accgctagat aatacatatg atttatcaaa tcgtcccaca gagaagtatt tttctcgtgc
    26641 accttgaagc catttttgtg aaacttcatt tacagcttga tgtaattctt ccggcagttg
    26701 atcgtaccaa gtaaaatggt atccttcttt catcaaacgt tgatattcaa gttgctgact
    26761 cagccgagac aagtcttctg ttgctagatc aacaatccct gcttcaccta ctttcgtgaa
    26821 gtgaaaaccc aattcatgaa gcatcactac gtagctttct gatatcttat aaaacgcaag
    26881 ctgatagcct aaacaatctg cttgtttcat aaactgattc gttgctgccc gcatcatttt
    26941 aggattgcca actggatctc ctaaaacaaa gcatttgttt cctttgatct gataaccaaa
    27001 aacgacttga tcttggtcgt cttcttggta ataaaaataa ctgtaacctg acaagcgaag
    27061 ctgatggctt gtagctgtgc ctccgtatgt ttccaataaa cggtcaaaac ggtctttttc
    27121 gtactcactg ccaagcttcg tagtatcttc agctaaatat tgataaagcg ccactaaagc
    27181 tagcaatgag atgcccaaac caataagccc tgagaaccac acatcatctg aaggaaacaa
    27241 aataaaatga tttcctgtta ttcgaccgac taaaaagcgt ccgctgtgat agcctgcaat
    27301 tgcgtaaacg ataaacaaaa agccgaacaa gctagcatca aagattatag ctccccaaga
    27361 ataaacgaat tttgttcgat aaaattcttt tctggataac cacacagcta acagaatcaa
    27421 gacatatacc aatatcaact gccaagataa cgtacgtgtg attgtattta aaataccaaa
    27481 agatagtaaa gcaatcgtag gcagaaatgc ctttttgact tttactgaaa gtgctctagc
    27541 aaggccgagt aataaaaagc caaccaatag attcactgtc tgatctaaaa aattgaatga
    27601 gaacggcaat aatacttgaa atagccgact gattgcagac agattagtga ttgttgatag
    27661 aagtaccatc atgatccccg caaaatagac agcacttacg agcacgatat gcgcaacctt
    27721 ttggaaaaac agtcgcggaa tgttatctaa aaatcgattg attttgactc ctgtatgtgt
    27781 gatgaacaat agaagtcccg tcataaaagg tagaacataa taaaacaacc gataatataa
    27841 tagccaaact actgctacat cttgccgaac agacaattga ctcattccta agatcatcaa
    27901 aacgtcaaaa gtccccattc cacctgggac catcgtcagc attcctatca atgtagcaat
    27961 catgaacata ggatagatcg aaagcatcga aatatctatt ctcatcaact tgccgatgat
    28021 caaaaatacc gcaagggctc ccgtccattg tccaaaggat gccagtagca gaccaagcaa
    28081 gccttttgga taaaactcga agaacaatgt ctttctcttt agataagcaa aaaagaaaat
    28141 gactggtgct aaaaaactac cgccgaaaag ccagatccaa tagcttcgaa aaatactttc
    28201 tgactgaata aaaaagacat caataaatgt aagaaacgac catacagaca aaccagtcag
    28261 cataaatagt gcgacttttg atacagtggc taaaaccatt tttctatttg tctgctttcc
    28321 ataaaaacta gcccgcagac ttgctcctac cacaccacca aaaccggcga gattgttgat
    28381 cgtgtttgtt acccacgcag caagaagaaa ctcttttctt tccatttttg gttttccttg
    28441 tttttctaag actctgatcg tcacccaatc atacaacaac atcggagtga cacccagcag
    28501 tccagccaga atcatgagca ggatcgtcat gcggctttgt tgtcccatcc tttgccaaat
    28561 atcttgccaa ctcatgccat tagcaatatt tgcgacttga tttgccacaa agactaacac
    28621 ggagccgaaa aaaatcagtt ttaaaatcag actatgttcc tttatccatt gcagcatcaa
    28681 gtatcgttta tttttcacat tttcccattc ctaacttgta tacttttgtg tcatacccca
    28741 atgttatctt atttatttta atttttccac tttagacctg ttctttgctt gcgatgatag
    28801 aaaaagaaag atatcctaat gctaaaggag aggataacat gaaagtagga tttatcggca
    28861 cagggaatat ggcacaagct attattgatg gtttattaaa aaaaggaaca gtctcgaaag
    28921 aagaggtgtt cgtttccagt ggacattttg aaaatgcaca aaaatttgct gctgaaactg
    28981 gcgtccatgc atgcaaaagt aatcaggaag ttgcatcaca atcagatatc gttcttttag
    29041 ccgtgaaacc aaaaatcatt gaaaaagtat tggaagaact aagagaatat agccagaaag
    29101 tcttattcat ttctattgct tcaggaaaat cattaataga tttagaaaca gcgcttgaag
    29161 atcctcaggc ctccatcgta cgtgtgatgc cgaatgtcaa tgtcagcgta ggagcaggcg
    29221 tttctgcgat ttgcaaaaat gaagctgcca cggaggaaca gcttcagcaa gctacgatga
    29281 tttttgaagc aatcggcagc gtctatcctt tagcagaatc agatttcagt acattcattg
    29341 gccttgctgg cagctcaccc gcattcgttt acatgctgat cgatgcgatg ggacggactg
    29401 gtgtactgaa tggtttgtcc aaacagacag ccactgagat tgctgcaaaa gcaattatgg
    29461 gttcttgtca aaaattcctt tcaagtgata aaaatccatg ggaacttgta gaccaagttt
    29521 cttctcctgg cggtacaact gttgcaggtg tagttgcctt agaagaagca ggattcatcc
    29581 cagcaatcat caaaggaatc aatacgacga tcgagaaaga tcttgagatg ttgaataaaa
    29641 aataatgata gcaatgtttt ttcgtgcctt acaaaaaaag aagagctgtg gcttttgccg
    29701 cggctcttct tttgtatttt ttattattta gcataatcag ttgcacgagt ttcccgaata
    29761 acgattactt tgatgtgtcc aggataatcc agttcttctt cgattcgttt acgaatatca
    29821 cgtactaaac gaacagcgtc taaatccgag atttcttctg gcttgaccat tacccgaact
    29881 tcgcgtccag cttgaactgc gtaacttgat tctacaccgg cgaagctgtt cgaaatattt
    29941 tccagacttc ttaaacgacg gatgtagttt tctaatgatt cacttcgcgc accaggtctt
    30001 gctgctgaga gcgcatctgc tgcagctaca aggacagaga taacagaagt agcttccaca
    30061 tctccatgat gagaagcgat cgcgttgatt actactgggt tttctttgta cttggcagct
    30121 aattccgcac cgatctcaac gtgagatcct tcaatctcat ggtcaagtgc tttcccaata
    30181 tcatggagca acccggcacg ttttgctaat tggatatctt cgccaagctc tgctgctaag
    30241 ataccagcta attttgcaac ctcgaccgaa tgcttcaaga cgttttgtcc ataactagtt
    30301 ctgaatcgca gacgtccgat gatcttgatt agatctggat gaagtgtatg agcaccgact
    30361 tcaaacgctg cctcttcccc gtattcgcga atacgttcat ccatttcttt acgagatttc
    30421 tcgaccattt cttcgatacg cgctggatgg attcgtccat cttgtatcag tttttccaag
    30481 gtcatccgtg cgatttctct tcggattgga tcgaatccgg aaagaacaac tgcttccggt
    30541 gtatcatcga tgattaaatc gataccagtc agcgtctcaa gcgttcgaat attacgacct
    30601 tctcgtccaa tgatacgacc tttcatttca tcattcggaa gggtaacgac tgaaacagtc
    30661 gtttctgaaa cttgatctgc tgcgcagcgt tgaatcgcta atgataataa gtttttcgct
    30721 ttgcgatctg cttcttcttt ggcacgctgt tcagattctt ttaccattag agttaactca
    30781 tgattcagct cttcttcagt cgatttcatg atgacatctc gtgcgtcctc ttttgataaa
    30841 ccagcaattt tttctagttc tttttgttga tcttcaatca ttttttcaac gtctttttct
    30901 cgctcttcaa ttaattgctg ttttgaacta agtgcttctt ctttactttc aagtgagttc
    30961 tcgcgttttt ctaacgagtc atctttacga ttcagcgttt gttcgcgttg aatcaaacga
    31021 ttctcttgag attttaattc taacttactt tctctcagct cactttcaac ttcagaacgg
    31081 tatttctgat tttcttcctt agcttctaac aatgcttctt tttttagagt ttctgcttct
    31141 ttttttgcac tatcgatgat tccagcagct gatgattgtg caccagctat ttctttctcg
    31201 tgacgtgact ttgcgaccat aaaccctaaa ccaagaccga caattaaacc gatgatagcg
    31261 aggagaatgt tgaataccat aaaatccacc tccatactat ctttttcatt tttatcatct
    31321 atacagcttc tatgataaat tattctaaac tcgtcaatat gcatcttcgc atatatgtgt
    31381 acaaaaaaca tgcacaactt tattctaatg tttgtacccc gtagtgtcaa ctttaaataa
    31441 acaaagaaac ggctttctac ttattcatta aacgatggtt tttaaaataa atgacactag
    31501 tttcatatag gtaaaccttt aaattgagaa acttttctat taaattattc tttgtagatg
    31561 aacagatatt tttattttat gtacccactc gtataattaa ataactcacg atttctttat
    31621 tcaataaaaa aacggcaggt caaggagagt acctactctt cagaccagcc gtttttgttt
    31681 atcataattt ttattcttct actaaaggca attcttcttg tcctttttca tcttctggga
    31741 cagcgacttc ttcaccaata ccataagctg ctctaactag agcagaaact tctgccatca
    31801 tttcaggatg attagccata tagattttag cgttttcccg tccttgacca atccgatctt
    31861 ctttataaga ataccaagca ccgcttttat caacaatgtc tttttctact gccatgtcta
    31921 gaagttctcc ttcttgtgag atccctaaac catacatgac atctacttca gcgattttga
    31981 aaggaggtgc tactttgttt tttacgactt tgatctttgt acggttacca acaatatctg
    32041 taccttgttt caattgttct gcacgacgta cttccaaacg gatcgttgcg tagaatttca
    32101 atgcgcgtcc tccaggagtg atttctggat tcccaaacat cacgcctact ttttcacgga
    32161 tttggttgat gaaaatagcg attgtttttg ttttgttgat cgaaccagag agtttacgca
    32221 atgcttgaga catcaaacgt gcttgtaacc cgacatgtga gtcacccatt tcgccgtcga
    32281 tttctgctcg tggaactaaa gcagcaactg aatcgactac tacgatatct acagccccac
    32341 ttgatactaa agcatcagcg atctctagac cttgttctcc tgtgtcaggc tgtgaaagaa
    32401 gtagttcatc gatattcaca cctaattttt gtgcatattg cggatctaac gcatgctcag
    32461 catcaatgaa agcggccgtt ccgccatttt tttgtacttc tgcaatagcg tgtagtgcaa
    32521 ctgttgtttt acctgaactt tcaggtccat atacttcgat gatacgtcca cgggggtaac
    32581 cgccgacacc taaagcgaca tctaacgcaa gagagccact tggaattgta gagatttgtt
    32641 ggtcgatttt ttcgcccaat ttcataatag agcctttacc atagctcttt tcaatttttt
    32701 tcagtgcagc atctaaggct gctttacgat catctgccaa tacgataatc ctccttatat
    32761 ttacaaatca gagcaacatt tcattatcct tatcatatct tttttagtga aaaaaagcaa
    32821 gaaaaaaccg aacaaatatt cgcatttatc attttcttaa aagctgtttt ctcaaaagat
    32881 caaatccttg cataaccgca ttatatctga tagcagcacg atctctggca aagtgatatt
    32941 tttccgaaac cacgccgtct ttagatgaaa tagcgatcca tactgttcct gcagattgac
    33001 cttctaacgt gtccggtcca gccactccgg tgaaagctac actgtaatct gttcttgcta
    33061 tctcacgtgc ttgaatggcc atctgctcca cacattcttt gctgactgtt ccatattctg
    33121 ctaatagctc cggatcaatc tccagaaaac cagcttttgt ttggagcgag taggtaacga
    33181 atccgcctgg aaatacttca gatacaccag caatgtcgcc taatgcggct tggaaggcgc
    33241 cggctgtcaa actttccgct gctgttacag tctgcttgtt ttctttcagt agttccacta
    33301 cgacttttgc tagcgaatta tcatcgccgt atccataaaa atattcccct actcgttctt
    33361 ggattttttc ttctaatgct aaaagagctt gattccctgc ctgaacatca tttgttttca
    33421 ctgtcagtcg taatgtcact tcatttggtt tggcatatgg tgcgatcgtt ggattgatct
    33481 gtgtctcaat cagatctttt agctcagtga ccagtcgcga ttcaccaatc ccataaaagc
    33541 gcaaaacttt agaaattagt ttttcttcag aaggaaaacg ttcctctagt aaaggacgta
    33601 cttgttccac aaacattggt ttcaactcat taggcggacc gggaagaagg atatacgcat
    33661 gtgtatctgt ttgataaaaa gtccctaaag caagccctgt acgatttggt agaggaaccc
    33721 cgccttcgat aatctgggac tgttgcagat tgtttttggt catttttcga tgggttgttt
    33781 caaaatatgc caataatttt ttgtatcctt ccgtattttg tatgagagac ttaccgaggt
    33841 gagccgcagt tgcttctttt gtcaggtcat cttctgtcgg tcctaatccg ccacataaga
    33901 gtatgagttc acttcttgtt tcagctagag caatcaactc ttctaaacgc tgctgattat
    33961 ctcctacaac tgtttgataa taaacttcga tacctagatc agctagctgt tctgacaaga
    34021 aagtggcatt cgtattcacg acttgtccta gtaaaagttc tgttccgaca gctattattt
    34081 ctgctttcat tacttcccgc tcccttatat agtatacgct gtatttttat atttttttat
    34141 ttaaaaagag ggcaaggagc tgtgacaaaa atcttgtcac agctccttgt tccaaataaa
    34201 cagtgttctc ctgccaaaac ctcggtcata agtcacaata tacataaaac atgaaaagct
    34261 gttttttgaa attatccctt gtgactcaga gttagacggc aggctcacaa cctctccact
    34321 aaacgatact tacattgatc ctttgaacac accgctgttt ttgatgaagt aatccacacc
    34381 tgaatagata gtaaagatca gacaagcata aagcatgatt tgatctactg gaataccgat
    34441 caggctgaat gggatattat tgataaacag caagataata gcaaccattt gtgtagccgt
    34501 cttgactttt cccggccaag ctgcagccat gacttctcca tgttcgacaa gtagtaaacg
    34561 caagcctgtg acggctaatt cacgacagac aatgatcgct actacccaac caggggcctt
    34621 tccttggtca accaacatga taaaagcagt cataactagc attttatctg ccaacgggtc
    34681 cgcaaacttg ccaaaattag tgacaaggcc tcgcgcacgg gcaatttttc catctaacca
    34741 gtctgtgata ctagctaaag caaaaataac tgcacctact agctgtgtga cagccagtgt
    34801 ggtgtctcct acgttaaatt gtccccaatc taaaggtaaa cctacaacca aaatgaaaat
    34861 cgggatcata caaattctaa gtacagttaa tttatttggt aaattcacac tatcgactcc
    34921 tttcttccct attctacgtg actatcagcg aagtttccac cgatattatt tacttttcca
    34981 aatgaattta ttcaaaggag tatttttatt gaaattgatc tttttgaatt aacgaagaag
    35041 gaccgcgaca cagatttgtc acgatccttc ttttttatac ctacaagtaa acagatgcgt
    35101 aaactaattt atacgatcat tctgcgtatt gaagagtaag gttcacattt tttggactct
    35161 gattgttttc ccctggatta actggtacgg attctccatt cgctttgatc gttgcattgc
    35221 ttgctgcacc aatcgtgatg gtcgcatttg ttgctgtttc cggtaacacg gtactctgtg
    35281 tttcatttgc tgccagtgtc ccttgataaa cataggcatt gtctactagc acacctaacc
    35341 atacacgatt gacagcagta aattcaaggg ttacaggctt ggtagcgtct gtgatattta
    35401 ctgcgatggc actgccagaa tcttctcctg tagctattgc cattttcttt tcttcacttg
    35461 attttgttgt tgattctact gtagaagact ctttggtctg cgtgctttgc tctgaggaag
    35521 aggactcctc gactgatcct tctactttaa gcgaagaagc tgtgtctccg attattggat
    35581 cagcttgacg atcttgccaa gtcatatagc ccacgacaac gacaatcgcc aaagcaacca
    35641 atcctaataa aatgacagga agacttgtcc aaaagcgact tggatgcgat tcttcctgat
    35701 gcattgcttt tctagaagta cttactgttt caggctgcgg acgttcaggg accgatcctg
    35761 tcaatttgct tttcccatca aaaacatcga ctaaatggtc tccatcttct cctacagctt
    35821 cagcgtattg ccgaataaat gcccgcacgt aaaatttacc aggcaattgg tcaaaatcat
    35881 cattgtcgat tgcttccaaa taacgttttt gaatttttgt aatctgctgt aattcatcta
    35941 aagatatttt tttattcagg cgtgcttgcc gtaatttttc tccgatggtt tcaaaagcca
    36001 ctcgttcatc ttctccagtc tgtttgattc tccgaagcat ctgctgttgc aacgaaagac
    36061 gttatgtttg ggtgctgtga caaaagtcat gtcacagctc cttttttccg aataaacatt
    36121 gttctcctgc caactcctcg gtcacaagtc acaatatcca ataaatataa gaagctgttt
    36181 tttgaacttg ttccttgtga ctcggagtta aacggcaggc tcacgacctc tttcttaaac
    36241 ggtgttcaat cagctgttcc tcggtattag ctcaaaaacc ggcaaaatat aaggagatat
    36301 ttttccgtat gtttctctaa tactcaggtc aaaacgctga tttcacaacc tcttccttaa
    36361 acggtgttca aaagcttgca tctgcggtaa taagtccaac caagttaaaa acattaagaa
    36421 gctgttttta cttggctgtt cttattactt gatgcagggc agctttttca caacctcttg
    36481 gtacacggtg ttccagaagt agcttcttcg gcaataagcc gaaatatcac aaaaatttga
    36541 gaagcaattt tcgtaaattc cttcttattt cttgaagctg accacttctg tcacaacctc
    36601 tttgattact tcgtttgatt ttattttaat ataagttgat taaaagagag catgatttgt
    36661 tttattttct cactaatttt tccaacacaa ctaagaatac cataaaagct gattttggag
    36721 gaggttccta ctacaagtct aaaacgaaat ttaagacttt tcctcataaa atactggttt
    36781 attttggtct catgtaaaac tcgctgaagg cctcttcact tataaatgct tccccagctg
    36841 ccagtacgtc tttcatttgg atcgaatcga tgatttcagg aagatcaaat aaagttcgat
    36901 cgccaaataa actttgcgta aattgattgg caatatattc gatcgaattt aatgattgga
    36961 aatattgtcc aagcattttc ttcttcagca aatcgagatt tgtttctgac acttccggat
    37021 catcagcgaa tccaagaatg atttctttga ctttttctgc tgctttctct ggttcgtccg
    37081 tatctccgct gaagtccgca aagtgaaatt cacgatccaa gctgaattca aaaccaaagc
    37141 tgtcatcgat aattccttga ttgtacatcg ctagatagtt tcgagaagta ttgcctaaaa
    37201 tcatttggaa caacagattg attgctgttt tatagcgtaa cagttctgtt ccttcttgcg
    37261 gcaatgtgtc taatccttta ataccaagta caaatttatc tcgtgtaatg gctgcttcca
    37321 atgcactttg tttgatgatt tcttttgtgt tttcagggaa ataacgtaca atttcttgct
    37381 ttggagggaa attttttgct tcttggtttt cccgaataag tttcatcaat ttctcaggct
    37441 ccatcttacc aactacaaat aaaaccatat tgcttggctg ataaaacgtc cgatagcacg
    37501 tatacaagtc ttgagctgtg attttgtcga tactttcaac cgtgcccgca atatcgatat
    37561 gcaatgggtg tgtaggatac aagttattta gtatgccaaa aaacatccgc cagttagggt
    37621 cgtcttcata catttggatt tcctgaccga tgattccttt ttccttgttt acagtttctt
    37681 ctgtgaagta aggtgcttgt acaaaatcga tcagagtcgt cagattcttt tctacctgat
    37741 ccgtcgtaga aaacagatag ctggttttcg taaaacttgt gaaagcattg gcggaagctc
    37801 cttgtttccc aaataattga aagacatcac catcttcttt ttcaaacaac ttatgttcta
    37861 aaaaatgggc aattccatct ggaactttca ccttttcttt ttcaccatag ggaataaatt
    37921 cgttatcgat cgaaccataa ttcgtactga acaaaccata tgtcttgtga tagtcatttt
    37981 taggcaacaa atacacggtc aaaccgtttg gcagtacttc atgataaagt gtttcattga
    38041 tttgctcata ttctgtttta ttcatttgca tttcctcctg ctaaaaagaa gattgcctgc
    38101 aattcgattt gctcagctac ttgttgtatc tctttgatcg ttacgctctc catacgtttc
    38161 aaccattctt catcagataa ttttgtttcc ggcagccagc tgtccaaaaa agaagcttcg
    38221 attgccgctt gcggagagtc tagagaaagc agatattggt ttctcagcat tgcttttgtc
    38281 tgagacagtt ccaactctgt tatttcacca tttcgcaaac tttctaattg ctcgtgaatc
    38341 agatgaagta cttgatttcg gttcttttca tcgataccgg tttggacact catgaatcct
    38401 cggaacgtat ccacgctact agaggcataa taagctagac tttctttctc tctgacattc
    38461 atgaaaagtt tagagtgagg aaaaccgcca aataaaccgt taaagaccat tagcgcaaaa
    38521 cgttctggtt catcgtaata cacatttgtc tgataagcta ggttcagctt tgcttgaacg
    38581 attggctcac gtacttgtcg ttccttcacg atattcactt gaggttgggt ataaaacaga
    38641 tctggataaa ctgtttcccg cgactcaaat ggtaattgcc caactgcttc agctgctttg
    38701 tccggatcaa catcgccgac aacaaaaatg tcgatttgat catttgccat catttcttga
    38761 tagtaggacg ctagcgaact agcagtgatc ttgtccaaat cttcgactac gcccaaccct
    38821 gggatctttt gttccgggct gttttcaaaa taaagttctt gcaaggcata ggaagcaaaa
    38881 gtttgtttat cttccttcaa actttccaaa tatagacgca gattgttctt ttccaagtca
    38941 aacgtcaact gatcaaactg atgattttta atatttggat aaaataaaac ctcttttaaa
    39001 aaatctaccg cttgagaaaa aaggtttgga tcattgacat attcaccatt cacaaaactg
    39061 atccctgcgt ttagccaatg taaatttcct ttttttctaa ctgataatcc aaagcttgcg
    39121 ccataaagct ctgcaagttg cgcacttaac tttgtctgat ccgaataatt caaactattt
    39181 gtttccaaca tgctgctgag aagtgaacgt ttcgtgatga cttcttgttg cagacgcgca
    39241 gtaaaacgga taaaaatccg gatcgttttg tattgttttg ttggtaagac atgaagattc
    39301 acgccttctg ccaattgata caccatattt atatgctcct tttcacagtt gattgcccgc
    39361 ttactctttt aatgggtcaa cctgcctttt ttacttattc ctctgcttga aatttataag
    39421 ttcaggcaat tcgccgtttt ttctaaatga tgttacactt tatagtatag catagatgtt
    39481 ttttatcaaa agaaacagac ggctaaaagc gtatattgtt tttttatttt ttatttttcg
    39541 atatactaaa gacaaataaa agaagggagg gtcattcatg atcaaagaat tcaaagagtt
    39601 catcatgcgt ggagatgtct tggatcttgc tgtcggcgtt gttattggta gtgcgtttac
    39661 aagcatcgta aaccaagtgg tgcaaggctt gatcacacca ctaataggtt ggtttgtcgc
    39721 aatgatcaca ggtacttctg atttagaagg agcactttca attcttgact ggtcgccgac
    39781 aaaaggcgtc acatttgcgt ttggtaacgt tattagtgct atcattacat ttctgatcac
    39841 tggttttgtc ttattcttag ttgtcaaagc ggcaaaccgt gcgaagaatt tacgctccaa
    39901 agaagagcaa gtggaagtgg aagaaactcc tactgcagaa gattatctta gtgacatacg
    39961 cgatcttctt gccaaacaag tggcagaaga caatacacat ccagtagagc aaacaaatct
    40021 ttccgatact tctaaataat aaagaatcta aaaaaaagaa agccgatttt cagtgtcggc
    40081 tttctttttt tagattctta acttgctact tgttcctttt tcatgtcgat atgtggaata
    40141 tcatcttcca aatacacatc tgagattgcc ttgaatccaa acgactcata aaaagcttgt
    40201 aggtattctt gtgcgccgat cacgattggt ttttctggaa aatttttctg gatcatgtta
    40261 atcgtttctt gaacaatctt tcttcctaaa ccatctttac ggttgtcctg atgaacaagg
    40321 acacgtccaa aatggatact ctccgcttct tcatatactc gcgtgtaggc aaggatcttc
    40381 ccatcagtat cttgaaaaaa cgtatgccaa gcttctccat caatgtcatc aacttcttga
    40441 taaggacact gttgttccac aacaaaaact gcgattcgtt ctttgacgat cgcgtaaaac
    40501 tctgctgttg acaattcttc aaatttcttt acctgatatt ccactaagca aacgccccct
    40561 gcttttttat tcttagcata ccgattcgca cacaaaaaag caaacgaacc ttacgaatct
    40621 caggctcgtt tgctttttaa actgcttcag ttaaaaattc gatctcaact gtccgagtaa
    40681 gtacatctga atagctgtaa gatactcgtt caaaagaatt ttcttcagga tccaaatcaa
    40741 caacgaatac tgaaggatag gtttctgtta aaacgccgcg gcgttctgtt tgacgctttc
    40801 ttcctgtctg agcaaccaac atgattgggc tgccgatacg accttctaaa tctttcttga
    40861 ttgaagctaa agttgttggc atacagttca cctctactat caactatacc acatattttt
    40921 aaaattttca agtttaccat aaaaaaaaaa gaaatgcaat aaaaataaat aagaagctca
    40981 ttaagatttt tctaaattat tcctcattat aaaatttcaa gtgttttttt attttgagtt
    41041 ttactctccc gtaagcgctc attacttgct tgtgatccat tgctaaacat tcagccatgt
    41101 ctacaaaatc aacgccttgc atataataat gaaaaatttt agcttctatt tttgaaaact
    41161 ggatttcgca attttcgatt gcttctttta ataaaagatg ctgaattgct aaaggttctt
    41221 gcaatgtgat ctctgctaaa aagtctggtc cttctgtctc tattttttgg tcgattgaga
    41281 cttcatcttt ttgatcacga cgcttcagcg cgttctgctt cctcaacaga gaatagattt
    41341 gattgcttat aacgctttta taataacttc caaagctaac acctttgctt ttttcgtaag
    41401 ttctcaatgc ctgatataaa gcgatccttg cttcttgttc ccaatcctca gaaacataat
    41461 ttcttaaata ataggtgttt ttcaaacgta gtaaaagtgg aaaatatttt tgatacaggg
    41521 cagcaaatgc ttcttcgtca ccattacgtg cagcgcgcaa taactcctca gacataaaat
    41581 aattcctcca taaattcgtt tatggaggaa tcttatcatc aatgattcat cgaactgatt
    41641 agaacgatat gttatttatt ttttctcatt aaatcgtcca atttttggga caattttttt
    41701 aactgctctt cattccatgg agaattacgt cggaaatcaa taaaatgcat atctttattt
    41761 tcttgggcta ttgttttttc cgtttttttt atcgtcttat ataattcata agcagaagtt
    41821 cgtaatgctc cttgggaaaa gatcatccac tgttctgcta aatcgcttgt cgccactgtc
    41881 acttgtgtca aacggtcatt cagctgacca gcgattctct cgatataact gtcagccgtt
    41941 tcgtcttctt tcgtaaaaac aacatccaac tggtatttct tataattttg ttggatgccc
    42001 ggaactagtt gggcatcgaa gacaacgatg acttccattc cttcatattt cgcataattt
    42061 gataaacgat gtaaaagagc ttctcttgca tccgccattt tgtcctgctt tttaagctgg
    42121 acaagttccg gccacgaacc aatcatgttg tagccgtcta caatcaacag ctgttttttc
    42181 atggtctact ccccctgacg ttgggaaaaa gcctgataca taagcaaacc agcagcaaca
    42241 ctggcattta agctctggac gtgcccggtc atcgggatcg tcagcaattc atccatttct
    42301 tttttcaatc ctggactcat tcctttgcct tcattaccaa tgactaaagc aattgatcct
    42361 ttggcattcc atttttggaa gtttgtgccg gtcatatctg tgccaaatac ccaaaatcct
    42421 gtttctttga gctgttgcac agcttgtacc aaattcgtca cgcgtgcaac agggacatgt
    42481 tcgactgccc cagtggaagt ctttgttacg ataggtgtga ttccaactgc tcggtgtttt
    42541 ggtatgatca caccatcgac gccacttgcg tctgctgtcc gtaaaatcga cccaaaattg
    42601 tgtggatctt cgatgctatc caaaatcaaa aagaaggggt cttctttttt ctcttttgtt
    42661 cgttcaatca gttggtcaag ggataaatat tcataaggtg taatcgcaag aacgactcct
    42721 tgatgcacgc cgtgatcgct caaagtttct aatttttgtt taggaaccca tttgactggc
    42781 acagcttgtt cacgtgcgat ttctttcagt tcttctactt tttctcctcg tgaatcttct
    42841 tgaagaaaga gtttattccc tcgcccttgt tgtagcgctt ctactgcggc atggtgccca
    42901 aagacgaaat tatcttctaa tgcttcttca ttttgatcag aaactgactg atttcgtcca
    42961 gtttgctttt tatttgaata tctattttgt tttttgtctt caaatccacg tttcgattga
    43021 tgtttgtttg ttctttcttt acccattttt ttcaccaact ttctgaatac accagcggat
    43081 caattcttcc atccgctcgg tttgttttgt caaatgcaga tagcccatga gcgcttcgaa
    43141 ccctgttgcg acccgatacg tcgtaacatc agcatttttt gcgctcgtat ggcttttagc
    43201 atttcttcct ctgcgataat aaagttcttc ttcttctgtc aaaagatgtt cttccagcat
    43261 ttgctgaatc aaaaaggctt gtgcattcgc agaaacataa ttcgttgccg cacgatgcag
    43321 cttattgggt tttgtttctc cttgatcaat caggtggtca cgaacataga tttcgtaaat
    43381 cgcatctcca acataagcca aggctaaacc gttcaattgt gtgtaatctc tcatgcttct
    43441 cttctccatc gggttccttg cggtgtatct tctaataaga tccctttttc tttcaacaaa
    43501 tcacgaattt cgtcgcttcg gacaaaatca cgattgcttc ttgcttgatt tcgttcttcg
    43561 atcaattgtt caatctcttc atctaataac tcttctgctg taaataaaat accgaagatc
    43621 gaaagccatt gcgagaataa agcttctcct tgtgccaaga cagcggcaga aactttcggc
    43681 tggtctagat aagtgttcag ccacttagca agctcataga ccactgtgat cccatttgct
    43741 gcattaaaat catcgtccat ttcttcgatg aatcgctgtt ctaaattttc taacaaagcg
    43801 attttatccg catcatctgc caaattctct gaggcagttt cttggcggaa tttcaaattg
    43861 tcaaacgtat tcttcagtcg ttgtaaattg gtggctgctt ctttcatcgt cgtttcactg
    43921 tagcggatcg gacgacggta ttgtgtggtc gccatgaaga aacgcaagac ttgtggatca
    43981 acgtttttga tcatttcatg tacagtgatg aaattcccta gagatttact cattttttca
    44041 tcgtcttcgc caatcgttac atagccattg tgcatccagt aattagcgaa ttttttccct
    44101 gttttcgctt cactttgtgc gatttcattt tcatgatgag gaaattctaa atcttgcccg
    44161 ccaccatgga tatcgatcgt ttctcctaaa tgttttgttg ccataacgga acattcaata
    44221 tgccaaccgg gacgtccttt gccccatggt gaatcccaag aaatctcgcc aggttttgct
    44281 tgtttccata aagcgaaatc taatgggtct tctttttgct gctgttcttc ccctgtacgc
    44341 tgacttgctc cgacttccag ttcatcgatg gattggtcac ttaatttccc ataattttca
    44401 aatttacggg tacggtaata cacatcacct gaagattcat aagcatagcc tttttcaacc
    44461 aacacttgaa taaatgctaa aatatctggc atatgatcca tgactcttgg atgcagtgta
    44521 gcaggttcga cattcaacgc tttcgtatct tcttcaaatg ctcggatgaa acgatcagca
    44581 acttctggtg ctgtaatacc taattcgttt gctgtacgga tgattttatc gtctacatcc
    44641 gtgaaattcg atacgaaatc gactttatag cctcgatatt caaaatagcg tcgaattgta
    44701 tcgaaagcaa tggtgctacg agcatttccg atatggatat aattatacac agtcggtccg
    44761 cagacataca taccgacatg tcccttttgt atagggatga attcttcttt ttttctggtc
    44821 aatgtattgt agatttttat cattctgatc tcctcacttt ttctcctttg attcgaacga
    44881 cttttgctgg tactcctacc gctgtagcgt cgtctggaac atctttcaac acaacggcac
    44941 ctgcaccgat tttggcgttt tttccgatcg ttaccggtcc taatatctgc gcattcgcag
    45001 agagcatggc tccttgttta acagttgggt gtcttttacc cgtttctttt cctgtgccgc
    45061 ctaatgttac gccatggaat aaaatcacat cgtcctcgat ttctgcggtt tcgccaatga
    45121 cgattcccat tccatggtcg atgaacacgc cagttccaat cgttgctcct ggatggatct
    45181 caacaccagt taggaaacgc cagaattgag cattgatctt cgccaataaa aagagccggt
    45241 gctgatataa aaaatgagag aaacgatgcc aaaacaaggc atgaaaacca gggtatgtca
    45301 aaacgatctc agctgtcgaa cgtgcagccg gatcgtttct ctttgctgca tcaatcgctc
    45361 gggtaaacca actcatgact ttccactcct ttcgtaagaa ttttatgtag gaaatcatct
    45421 tcttataaag aaaagaagcg ccttttaaat aagtttcctt atcaaaagac gctttccgcg
    45481 tggttccact tttcttcata atcaatgatt atctctgaca gctaacgcct gttatacgtt
    45541 cttggctact ttgtttccta taaaacagtt tttcctgttt tctgtgtttc gccaagacca
    45601 ctcccagagg catttcggca accattcatt gataccttgc accacccggt atctctcttg
    45661 taatgatcag ctgcttactt ttttctgttc atcgtgttta gttcagtact tgatttaaat
    45721 gtgctaatga tttttcacga ccaagcaatt cgatcgtttc aggcaattct ggaccatgca
    45781 tttgtcctga tacagctaca cggattggca tgaaaagatt ttttcctttt acgccagttt
    45841 ctttttgaac agctttgatg gcagctttga cagttggagc atctactact tccatttgtt
    45901 ctagctgtgc tttgaatgcg tggagaacag taggaactgt ttcaccagca agtacttctt
    45961 ttgctgcttc attcaataca ggatgttcat tgaaaaatag cgtagacaga tcaacgattt
    46021 ctgcagcata gctcatttga ggttgataca ggctaacgat cttcttcacc cattcgattg
    46081 tcttttcatc tgggttttct gctacacgtc catcagcgat tagataaggc agacacatgt
    46141 cagtaaatgc atctagatcc attgctttga tgtaatggtt gtttacccat tgcagttttt
    46201 tcgcatcgaa agctgcgggt gatttactta ggcgttttgg atcgaacatt ttgatcaatt
    46261 cttggtgttc gaagatttcg tcttcgccca caggtgacca gcccaataat gcaataaagt
    46321 tgaacattgc atctggtaga tagcctaatt cgcgatattg ttcgataaat tgcaagattg
    46381 attcgtcacg tttgcttagt tttttacctg tttcactgtt gatgatcaat gtcatatgtc
    46441 cgaaggttgg tggtgtccag ccaaatgctt catagatcat cagttgtttt ggtgtattgg
    46501 caatatggtc atctccacgt aaaacgtgag tgattttcat caaatgatca tcgaccgcaa
    46561 cagcaaaatt ataagttggc ataccatcgc gtttttggat gacaaagtcg ccaccgatat
    46621 tatcggattc gaacgaaata gcgcctttca ccatatctgt aaatgcgtat tctgtattgc
    46681 gaggcacacg gaaacgtaca acagattcta gaccttgtgc ttctttttct gcttgttctt
    46741 caggtgtcaa gttcgcacat gttcctgcgt aatgaggcat ttctcctctt gcgcgttgtg
    46801 cttcccgttc tgcttctaat tcttcttctg tgcaatagca tttgtatgct cgattgctag
    46861 caagcagctg atcaatcagc ggttgataga tttctttacg ttcagattga cggtaagggc
    46921 cgtattctcc aggattttca ggagattcat cccaattcat tcccaaccaa gcaaggtttt
    46981 ccaactggct tttttcgcca tcttcgatat tacgtttttg gtcagtatcc tcaatacgaa
    47041 taataaagtc tccatcattg tggcgggcaa acaaataatt gaataatgca gttcttgcat
    47101 ttccaatatg caggtgccca gttggacttg gtgcatagcg tacgcgtact ttcgtcattt
    47161 tcttcctctt ctttcaaact agactttgac taacagcaaa aattccgtca gttacggctt
    47221 ctcaaatatc atttatttaa agccactcag cacaaatttt accttttcta aggtaaatag
    47281 taaagccaac tggttgaatt tccttaccag ttggctgaat ttctttttta ttttgattgt
    47341 ttagtctttt tctctgtctc ttttttattg tcgattcctc gtcctgcatg tgcaggttta
    47401 gcaaagatca tgcgtccagc agctgtttga agggcacttg ttacaacgac ttcgatacgt
    47461 tcattcatgt aatgctgtcc atcttctacg acaaccatcg tgccgtcatc aaggtaggct
    47521 actccttgtt ggcgttctgt cccggctttt actactagaa cattcatcgt ttcaccaggg
    47581 atgacgactg gttttacggc atttgccaac gcattgatat taagaacagg tacattttgg
    47641 aattcagaga ctttattcaa gttatagtca ttggttacaa tgacaccatc taaaagctta
    47701 gccaatttga tcaatttgct gtctacttca ctgatatctt caaagtctcc atcgtacatt
    47761 tcgacagata taccctcttc tttttgcaag gcatttagga tatctaatcc gcgacgacca
    47821 cgcacacgtt tcaaactatc ccctgagtcc gcgatgtatt gtagttcgta aagaacaaaa
    47881 ttagggatca tcaaggttcc ttctaagaat ccagtctttg taatatcata aatccgaccg
    47941 tcaatgatca cgcttgtatc caagatttta tatttatgga aattttctcc tgattttcgt
    48001 tctaacagtt gactctcaac ttgactagct gtttcttgct cgattttttt gcttcgtgaa
    48061 ccaaaaactt tcttccattc ttcgattcgt gtcgttccca ttctcaatcc cagatagcct
    48121 aagatgatca tcagtaaaat cggcaataca ctattgacga atggaatttc catattgtaa
    48181 agaggggtcg agatcacaac acctaaagtc aatccgatga tcgcaccgat cgcaccgaag
    48241 agaagataag tcaagctgac ctcacttagt ttttgttcta cttgtttgat aatagctgag
    48301 atatgtttgg ccaacagcaa agataataaa aagaaaataa gtgcgcctaa caggctgttg
    48361 gtaaatgtat tattcagcca tccgtttgac tgttgaccga ttgcctgcca agctgagggt
    48421 aaaaatgaaa tacccaagct tgcgccgaca atgatcatca gcaaagtaat gacacgtttt
    48481 tgcatcgttt ttcctccgtt tcgacaatca tttattagtt agcggaacac acgcttcaac
    48541 gtttcagcca aagttgctac tccaactatc tcgattcctt ttggcgcttc ccagttacct
    48601 aagttgtttt taggtaggta tacttttgtg aagccgagtt tttggacttc tttgactcgt
    48661 tgttcaatat gactgacacg tctgatttct ccagtcagtc caatttcacc gatgaaacat
    48721 tccgttggct gtgttccttt ttctttatag cttgaagcaa tactgacagc gatagctaaa
    48781 tcaatcgctg gttcatttaa ttttacccca cctgcagctt ttaagtaagc atcttgattc
    48841 tgaagaagca acccagctct tttttccagc actgccatga tcagagatac ccgattgaaa
    48901 tcaagacctg ttgttgttcg tttggcattt ccgaacatag taggtgtgac taatgcttgg
    48961 atctctacca aaattggacg tgtcccttcc attgccacaa cgatagcaga gccagttgca
    49021 ccgtctaggc gttcttctaa gaaaatctgt gaaggattag cgacttcttc caatccatgc
    49081 tcatgcattt caaaaatccc aatctcattc gttgagccaa atcggttttt tactgctcgc
    49141 aaaatccgaa aactatgatg ttgttcacct tcaaaatata acaccgtatc caccatatgt
    49201 tctaacattc ttgggccggc gatcgagcct tctttggtaa catgaccgac gataaaaatg
    49261 gctatcccat ttgttttagc ggttttgagt agttctgcag tggtttctct tacttggctg
    49321 acacttcctg ccacacttgt gatgtctggc tgcgtcatcg tttggatgga atcaatgata
    49381 acataatcag gggagatatg ttcgatggca cgactgattt cgttcatatc tgtttcagcg
    49441 tatagataaa attctgtgtt gatacttccg aggcgttccg cgcgtaattt tatctgctct
    49501 gcgctttctt cgccggatac atataagact tttccgccta tggctgctaa ttgctgagaa
    49561 acttgtaaca gcaaagtgga tttcccgata cctggatcgc caccaatcaa gaccatagac
    49621 cctggcacca cacctccgcc taacacacgg ttcagttctt ctaattttgt tttgatccga
    49681 ggttcttttt tagggacgac gtctgcgatt tttgttggtt ttgctttttc tcctgtcaaa
    49741 cttgtccgag ttctgcggtc cgttgtatct tgttcgattt cttcgaccat cgtattccat
    49801 tttccgcaat ttgggcagcg tcccaagaac ttaggcgaca catacccgca attttggcaa
    49861 acaaattgta cttttgattt ttttgccatt gtaccgcctc cttttatcct aatcatgaga
    49921 cattaaatca agcttaagta tctttaacgt tttatttact ggaagaacca aatccgcctt
    49981 gacgttgagc agtcgtttga tcttgatctg cttttaaaaa cggcaaaaag atcccttggc
    50041 cgatacgctc gccttttttg atcactacat ccttgaaacc aaagttggtg aattggaaca
    50101 tgatatggcc ttcatttgcc tcattattgt aataatcact gtcaatcaca cctactccgt
    50161 ttgtcaacac taaaaaccgc ttcaacggat tgcttgaacg gttagcaagc tgcaaaaatt
    50221 catcttctcc catgtaagct ttgattccag taggcacgag ggttggtttc aattgttttt
    50281 cattttctga tacatctttt ttcaatccat ctgcgactgt ttccaacccc tttttccagc
    50341 ctgctttcca gatacttgga atcacgacct cttctgctgc ttcaaaatca tagccagcag
    50401 catgatgtgt agcgcgctgc ggtagattga tattttgatc tttatattta gaaataactt
    50461 caaatcctct ttttctcacc ggttttcctc cctaaataaa ctactcctat tctaacatat
    50521 ccgaggctgt aaaagacttt ctttacaaat tcttcaagaa attctgctat cttaaagtaa
    50581 accattaatt cgttcttttt ccaaacgact gcaagttgta caaaaaaact attttttaca
    50641 tgactttact cgtcaaaaca agtagaatag aacgaaatta atgagtggat atttgattga
    50701 ggtgtcacat gcgtaaagaa attaaaaatg caaaacgaat tattgtcaaa gtaggaacaa
    50761 gtacattgat ttatcccaat ggaaatatca atcttgcggc aatcgatcag ttagcttttg
    50821 tcctttctga tcttagaaat cggggaaagg aagtgatctt agtctcatca ggagctgtag
    50881 gcgttggtat gcataaatta catttgtcag aacgtccgac tttgattcct gaacagcaag
    50941 ctgcggcggc tgttggtcaa gccgaactga tgaatatcta taatcaaaga ttcagtgtat
    51001 ataatcaaca gacggcacag atcctcgtga caagagacgt ggtagattat cctgaaagtc
    51061 gtaaaaatgt gaggaataca ttggaacagc ttttgattat ggacacgatc cctatcgtga
    51121 acgaaaacga tactgtagca gtggatgaat tagatcatct gacaaaattt ggcgataatg
    51181 atcagctgtc agccatcatg gcaaaacttg tcgcggcaga ccttttgatc gtcctttctg
    51241 atatcgacgg gttttattca gataatccga ctacaaatcc cgaggctcag atgtatcgct
    51301 atatcgataa aatcgatgaa aagattctgg cacaagcagg tggtgctggt agcacatttg
    51361 gaacaggcgg tatgcagagt aaattaaaag ccgcttctag gatatttgaa gcaaacagcg
    51421 caatgatttt agcaaatgga aaaaatccgg caattatttt tgatattctt gcaggaaaag
    51481 aagtaggtac tttatttaag gaggacttga aatgactgac ttgatgcaat taggacaaca
    51541 ggcaaaagat agcgcgcaac aactagcact tatgcctaca aaccgcaaaa atgagctact
    51601 tgttcaaatg gcacaaacca tcaaaaagaa tcaacaagcg atcattgaag caaatcataa
    51661 agacttagaa aaagcagtcg aaaacaatat cagtgaaacg atgcaagacc gcttgcagct
    51721 gacacctgag cgaatcaatg ccatggctac agagatcgaa aaaattgctt cgttagacga
    51781 tcctattgga aaagtagatg agatgtggac gaacaccgac ggattgagaa tcggcaaaaa
    51841 gcgcgtgcca ctaggcgtga tcggcatcat ttacgaatct cgtccaaatg tcacgacaga
    51901 tgcagctagt ctagcattca aatcagggaa tgccgttatc cttcgcggtg gcaaagaagc
    51961 attcttctcg aatcaattat tagtccaatt attgcagcaa gtactggtca gtgctggcga
    52021 atctccttat gccatccagt ttgtcgatga tacttctcat gaaactgcag gacagttgat
    52081 gcgattgaca gaatacttag atgtgctgat tcctcgaggc ggagcaaaat taatccaacg
    52141 agtcaaagaa caggcaacgg tacctatcat tgaaacaggt acaggaaata atcacgtata
    52201 tatcgataaa gaagcacagt tatctatggc agtgaacatc atcgtcaatg caaaagcttc
    52261 ccgtccatct gtctgtaacg cggcagaaac attgctgatc cattctgaga tcgcaccttt
    52321 tttcttgcca gctattgaaa aagaactggt agaacacggg gtctctttac gtgcggatgc
    52381 tcgtgcgtta gaatatctag agacggctgc tttagcagaa gacacagatt gggatactga
    52441 atatctagat tatatccttg ctgtcaaagt ggtcgattct ttggatgaag caattgcaca
    52501 tatcaatcgg cataatacta aacattcaga aacgatcgtg acagatagtt atgcggctag
    52561 tcaacgtttc ttaaatgaag tggacgcggc ggctgtctat gtcaatgcct ccactcgttt
    52621 tacagacgga tcggtatttg gttttggtgc agagattggt atctctactc aaaagttgca
    52681 tgcccgtggt ccaatggggt taaatgaatt aacttccaca aaatatatca tttatggcga
    52741 tggacaaatc cgcgaataat gtttcgtgtg aaacattatt cccgcctttt gccttttttc
    52801 tgtaaaggtt ctatccaact agaaaggtgt caatatgttt gaaattttaa tgattttttt
    52861 tatttacctt atttctctga atatcgctgc ttttctggga gtcggtattt tatccctttt
    52921 ctttcaattc aaaaaacgaa gcatagggag tcaacgggaa aaatggtcgc agtattttga
    52981 caaaatcggg caaaaagggc tggttacacg cttgcacatt agttacatgg ttgcattatg
    53041 cttattagcg acaatcaatt actattcttt tttcgatcat tcaatcgctt atacgattac
    53101 gctactgatt gctgggattt ttcacttatc ttataaatat cagctgaata aaaaccactt
    53161 gaatcggact ttccgctaaa aaatatccgt acgataagta gagtgccttg aagatcggca
    53221 aaaaactgcc ggacttttta gaggctgtga caaagatttt gtcacagcct ctttttccat
    53281 ataaacggcg ttcgcctacc agctgcctgg taaatctgtt tcctcttcgt taagaagcga
    53341 aaatccgtca aatgtttcgc catcataata acgaagcagc tcatccatgg acaagttcat
    53401 cgttttttct ttttcttctt ccgctgattc agccagtagc tgttcccgat acacctgata
    53461 cataccagct attttttgct gcatcgcttt tctagtttca ccttccgcat tgattattgg
    53521 caattctttg atatagccac gataaatcaa ccgatcatct gaaaattcaa tgggtaattc
    53581 aacgagtgtc aacccgactt cctgaaaata ttcttccagc atctattatc cccccattct
    53641 ttttcttaaa tgaattttag cttacctact gaaaattttc cagtttcatt attttgagaa
    53701 aaaaagggaa tgtgacaaaa atcgtgtcac atcccctttt tccgaataaa tggtgttctt
    53761 ctgccaactc ctcaatcata agtcacaata ttcaacaaac atgagaaact gttttttgaa
    53821 attgttcctt gtgattcaga gttaaacgac agtctcacaa cctcttggta aacggcgttc
    53881 aatcagctga ccctcggtat tagctcaaaa aacggtaaaa tatgagaagc tatttttccg
    53941 ttttgtttct ctaatactta ggataaaacg ctgattttac aacctctgtt aaacggtgtt
    54001 caatcagctg tccttcggta ttagctcaaa aaacggcaaa atatgaggaa ctatttttcc
    54061 gtttgtttct ctaatactca ggacaaaacg ctgattttac aacctcctga taaacggtgt
    54121 tcaaaagctt gcatctgcgg taataagccc aaccaaacga aaaatatgag aagctatttt
    54181 tgtttggctg ttcttatgac ttgatgcagg acagcttttt cacaacctct gatttcttat
    54241 tcttcttcat ttgtagcaaa ataattacgg tgataaatga tacaacatac actgataaga
    54301 acaatggcac ctgcaaaata aaacgcatag ctttcaccaa tatgttcacc aatcacacct
    54361 gcaataaatg gtgctaaagc tccaccgaaa aagcggacaa aactatagct ggacgaagca
    54421 acgtttcgtt gcagacctgg tatttccata gcaatcgtcg tcaacagtgt attgataatt
    54481 ccttggaaaa atcctgctaa cacaatgctg atcggtacaa ggactggcat agaagttcca
    54541 gctcctaata acagcaaaca aataatgaat aagacaaatg ctgctaaaat tgtccgataa
    54601 gtcgtaaact tccgctcaag agccggtgca acgaaaatcg aagcaatagc cagaagcaca
    54661 ccccagccaa aaaatacaaa accgacttct aattcagaat agccaatcaa taaaaatggc
    54721 gcatacgcaa gtaaagtaaa gaatccgaaa ttatataaca atgcgattag tccaactgaa
    54781 cgaagtttat gattgcttaa cgctttgaca ccttcccaaa ggcctacttt tctctttggt
    54841 ttctcagttt caggtaaaag taccgctagg gctataaaac cgcaaaacat caaaaatgct
    54901 accccaaaaa atggataacg ccatgaacca ctgcccaaga ccccaccgac tagagggcca
    54961 acagccattc cgcagccaag tgctgcttca tacatcatga ttgctttttc tgtgttacca
    55021 actaaaatac tgacgatcgt tgctaaagca gtagagacaa ataaagcatt cccgacaccc
    55081 cagccggcac gtaacccaat cagcattccg atattcccag ataatccgcc aagaccagcg
    55141 aatacaacga tgataaacaa tcctgtcatc aatgtttttt tcaaaccgat ccgactagat
    55201 aagaaacctg taaatagcat caccacacca gtgactaaca tgtaactagt aaataacagg
    55261 gtcgtttcag ctggcgtagc gtttaactgt gcagcaatcg tttttaagat tgggtctact
    55321 agacccactc ccataaaagc gatcatacag gtagctgcta caactagcgc tgaacttttc
    55381 ccctttgttt cttcatttgt ttgttcttgt atatctattt gattctccaa tatttccatc
    55441 cctttctctc tattagcatc atacatgatt tattttacat gtcaatatgc acatgtaaaa
    55501 cgtggaaact ttactgtgat ataataaaga aaataagacg agaggtagga tttttatgaa
    55561 tactttagcc tatatattat tatgcgtgct tttatccaaa ccacgttctg ggtatgaatt
    55621 gaagcagctg atcacaattt tttgggaagc ccatcacagt cagatctata caacgcttgc
    55681 caagttagac cagcagggtt atgtgaccgt tttggacaaa gacgagcact ctcaaaaaaa
    55741 gatctaccac ttgactgatg aaggacgtgc tcttgtagag gaatggatca aagaggaaac
    55801 tcccgctcct acgcaaaaag atgaatttct agcaaaaatc tatgcttttt cgacgctcga
    55861 taaaacaaca gctactggac tgctattcac tagagaacaa cagctgaaca aaatcatgca
    55921 aaaaaatcaa aaaaaattga atgacctctc gctagacaag aaacaagaat ttggacgtta
    55981 tgttgtgatc cagcgcagaa ttatgctttg tcagcaggaa ctccaatggt gtcagcaagt
    56041 aagagaacaa atgcagcagt tttttgagta agggataaac agaacctttc tagaaatatc
    56101 tattgaagag caaattatct tcaataaaag agcaatacag ttggaaaaga aaaagcgatc
    56161 cgaacgacag gccaaaacct atcgttcgga tcactatttt tctagaactg cattcaagag
    56221 actatgacaa aaagcatgcc aaggcctttt ttcaaataac agtgttccat cattcattac
    56281 tttgaattgc ttttagcaca cttcttgaaa gcgggccaac gatgatcaac tgcaacggta
    56341 aagcggcaat cacattcatt ttccaagcat tcatataagc agagaataat tgatcaggta
    56401 ttccttgttc catgatcaca ccgaataaag acatacatgt caccattcct aaaaccatca
    56461 agcatgaaat agttaagatc atttggacct tgctttctgg atttactggt agtttaaagg
    56521 caattttttt cgccagcaca ccgatgatga agacatccaa aatgaaagcc accacgaatc
    56581 caggcactag accaatcaac aaacttgata aatgaagatc ttgatgcagc cataaattat
    56641 acgcgctcat ccccaagacc atcaaaaaac acatcattgt tgtaaaaatt attccttctt
    56701 ttttatttgt aggcaatctg taccactcct ttaaatttgt cacgaaaatc atcgtagcac
    56761 atttaaaaat ttctcgtaag tacttttttt attttctgcc cttttatttc ataaacttca
    56821 tcagccgtac gctcgacaaa gtaagaatca tgagaagtaa acaaaatcgt tcccggatat
    56881 atttgcatta gcttctctaa agcttcgatc gttgccatat caataaaatt agtcggttca
    56941 tctaacagca acacattact tggcttagta aaaagcaaag caatcgtcag acgggtcgct
    57001 tctcccccac ttaatgtaca taacggtttc gtcaaagttt cctgagcaaa ccctaagtga
    57061 ttcagcaaac tacggacaag cgattctgaa aactctgttt gtctcatgac aaaacgaatg
    57121 atggattctt cagatgtcat ttgataaccc ttttgctgat atacttgaaa gcttactttc
    57181 ggagagagaa ggattccttc tccttgtttg cggatatgtt ctaataaggt tgtcttacct
    57241 gaaccatttt caccgacaat cgcgattttt ttacctaaag aaaactgaaa atcaccattt
    57301 accagtaatg ttctcccact tctttccaat tggacatttt gtcccatgat gggatagcgg
    57361 ctgtggagtt caagagattt tggtactgga aaacggattt gtttcatctg ctgcggcttg
    57421 gttgtttctc ctattttttg gagtcttctc tctaatgttt tcgcattctt ttggatggct
    57481 ttttgtacac tgtctttttg tttagaggaa gacaaccgat ccagtctgat ttgttgcttt
    57541 ttctttttac tggacacttt ttccaacttt tccgcttgtt ttttcttttc ttggatcgat
    57601 ttttccaatc gttttttctc tttcatgatc gactctgctt ctcgtagctg agtctttttc
    57661 tccaattcct tttgagtgag ataggcacta taattccccg aaaactctcg gatttctccg
    57721 tcttttactt cccagatttt cgatgctagc tcatctagaa aatagcgatc atgactaaca
    57781 aaaaccagcg taccataata ataacgaagc tcttcaatca gtcgatcaat actttttcta
    57841 tctagatggg ttgttggctc gtctagaagc atcccttgtt cataagaaga aagaagacgt
    57901 atcaactcga tctttcgttc ttcccctccg ctccatttcc gctctgaagt ttcttccagt
    57961 cttaactgga aatgactggc taattccaaa tttaggtcct ttgcttcagc gagataggtt
    58021 aattgaggca ggtagttgaa ggtgatctct gtttggattt tcccatgatc gggaaaaagt
    58081 tcaccggcta ttagtttgag caaagtcgat ttaccgacac cattttctcc tatgatccct
    58141 atacgatctt gctgatagac acgaagagaa tcaatagata gttctagctg gttgccaaag
    58201 ctgacttgta gatttgttat atttactgct aaattttcca tatgttttac actcctattt
    58261 catgtaaaac aacaattatc gatactaaaa aagagcagaa aaatctgttt gcaaaaaaac
    58321 tgcatcacat cttttcctac tctgaacgaa ataactatga ctaaatcacc aaatctgtca
    58381 tagctttcac ctattctact gtcgttcgaa aaaatggaca tactccatcc atgaaattgt
    58441 tgtttataca tgtcttttga atttatttag ttgttattca aaagttcgaa acgtaatttc
    58501 atcgatgcag tcatttttcc gacctccttt tttattaggt taccacacac gattcaaaaa
    58561 aagcaatagc tactgaatag taactgaaaa ccttttattc gaacatagtg atcttttctg
    58621 gtgctaaacg aacagactcg taaccttctt ctaccgcttt tcctattgat aaaataataa
    58681 ctggaacata acggtcttta tccaaaccaa atgcctctgc caactgatct gcttcgaatc
    58741 caccaattgg atttgtttca tagccatgat cacgcgcaac taacatgaat tgcatagccg
    58801 ctaagctaga atcgactttg actacatcgt tcatttcttc tcttgaaaaa ctcttgtaat
    58861 aaggaatgat cgcagctaat tgttgatctc tgacttcttt tggcatcttc cctgattcgt
    58921 aagcttggtt gtagatttct tcgccatatt cgtaacattg catatcgcca aaaatcatga
    58981 ccattgcaga agaagtatca ttttgtctcg tattgaaacg gatcaaagga cgcaagattt
    59041 cttttgcttc ctcgctttca gcgaccacaa agcgccatgg tttcatattg acagaagaag
    59101 gtgcagtcgt cgcttctttg atcatttcca gcatttcttc gtgtgggatt ttgtatgttt
    59161 catcatatac gcgaacggat cttctaccag tcataattgt tgaaaaatca tttttttgtt
    59221 ccattattat taacctcttt cgtttctgtt gttatattgc aaagttgggt attcaattta
    59281 tctaaaagtg ttagaaattg tttcatttct acatcgctga aagccgggga tagcacggaa
    59341 cctgtatctt tatggtccat ttcacaatgc ttgattttct tctgggcttt ttctgttagc
    59401 gctacataca cttcccggtt gttctgagga tttctctgac gcgaaacata gccatttgct
    59461 tccaatacct tcagatgacg agtaactgct ccttgatcaa tctgcaactt ttcttgcaaa
    59521 ctgctttgta gcaatggatt ttcttctttc agtctcatca tgatctcgta tctagtaaga
    59581 cttataccga tattttcttc aaagatctgg acgatttttt gatcggctaa tttgatttga
    59641 tataaaaggt gactaatctc ttctaaagac ataatttcct cctttctaat aattgactcg
    59701 tcaacgattg acttgtcaat aatagaataa aaaaagaaaa aagtcaaaga atcagctgtg
    59761 taataaaaaa aacgctctca atagaaaagt atagagaaaa gaaatttttt gtagaatgaa
    59821 cggaaaaaat gataaagaaa gcacaacgct aaaaccaaga ttattgattc ttatcaaact
    59881 atagaagata gctcttctgc agtagataat tttacaccgc caaaaatttc cgaaatcgcc
    59941 ttttgcaaga atcccgtcga atattagaaa ttggatcagt atgcttaaac agtaataaaa
    60001 aagttatgac aagatgttgt cacaaccttt tttgagtaaa cggtgttcaa aaacttgcat
    60061 ctgcggtaat aagttcaacc aaacaaaaaa catgaaaatc tgtttttgtt tggctgttct
    60121 tactacttga tgcagggcag ctttttcact acctcttttt aataaccata aaatcctatt
    60181 ccttcatatc tccagcccat cggtgttagc caatctcttt ccttagcact caaggtatag
    60241 tgatgggtac cagttttttc atttggatta aatagtcgat aaattggctt ggaaccacca
    60301 gaataaaaac ttagaccttc ataacgccaa ccgatttttg ttaaagagtt gatctcagat
    60361 tgtgccattg tgtaatgatg gtctccactg taaggattgt ataaacggta gacaggttgt
    60421 ccgctgtttg gtgcttgcca agcaatccct tcatattccc agcctacagt tggcagataa
    60481 tttttttctc cggcattcaa tgtatataaa tgttctcctg tatgacggtt atacagacga
    60541 tacatcggag aagttgaagt tggtgctgta tcatactgtg tcagattata ggtttcgatt
    60601 acattgttca atgtagaacc ataattaggc gcagtggcat aacgacctgt tagccaagcc
    60661 gtagcatctt tgtaagagtt cgtattgctt ttccatgcac cggaatagta gtaaacgcct
    60721 ggctgaaaag aagtcgtctt caataccgtt acgttatctt gcaatgattc tttgtacgaa
    60781 ggatattttc taaaagcggc attcaccgta atccattgcc cattcacata ttccgaagta
    60841 ggcatcgtaa cagattggcc attatagctt cctttaatcc caaatagatt gtaattaggg
    60901 gcagaagcca aagtactgtt cccccagcca ctttcaacga tggcttgagc aatcatgact
    60961 gatgcatata agtcattggc acttgccaaa ctttgtgcag ttgaggcaat actatcaatg
    61021 aacgcttgtt gagaattagc agatgagcga gcataaaaag catagccagt atagttttcg
    61081 aattcagaca aatctgcttg atgggtatgt gtcaatgttt gcccatctgt caattggttc
    61141 gtgatttctt ctgcattctc tggattgttc tgagcttctg ctgatgtact gtcttcggtt
    61201 tgtgtttgtt ctgttgtttc agttgttggt tcttcggttg aaattgtatt ttctgagaca
    61261 gtagctgact gcaaagtact ttctgtatcg ttttcaattc ctgtttctga agaactcgat
    61321 gtctgagttg cgacagtttc tgtcgattct tcagctagca ctgttgtgtt ggcaactgct
    61381 agatttccta aaagaataac gctaaataat aaattctttg ctttcatctt ttttctcctt
    61441 tgaattatga aacttttcct aaataacagt gtaactaaaa aagtaaaagc aagtcaatgt
    61501 ccatatttaa ataacatttg tttcttttta ataaatttgc tttttctgca taaaaagacg
    61561 taaatgaaga atattcttct ccattcacgc cttaaattga tggtcgtttg aataactatt
    61621 taacaagtaa ttctctctgg atattatccg ttatggatta tacttcctgt gtcgtcggac
    61681 ggataatcat gttgctgaca gacatccggt ctggcgtttc tatcgcaaat gctacagcag
    61741 aagctatatc ttcagatgtt aatccccatt ctttttgcgc ttcatgaagt tcaagagcag
    61801 cctttttgtc gctgatggtt tgatataatt cagtttgaac agctcctgga gagatgatcg
    61861 tagatttgat gttattttgc cgttgctctt gacgtaatcc ttccatgatt gcacggacag
    61921 caaattttgt tccgcaataa acggcggaat caggataaac aacatgtccc gccacagaat
    61981 cagttgaaat gatatgccca gatttttgtt tttccatgat tggaagtacg gcggaaattc
    62041 catttaacac acccatgatg ttgatatcaa gcatattttg ccattcatca cggtgacctt
    62101 caactaatgg agcggttggc atgattcctg cattattgta caatacatcg attcgaccga
    62161 acttttccat cgctaaatcg ataactgctt ggacttgtgc aaaatctctt acatctgctg
    62221 tttgaatata tagctcagct tctggtaaag attcttttat tgcttttagg cgatcttctc
    62281 ggcgtgcagc tataactagt ttagcccctt tttctgctaa tagcttcgtt gttgcttcac
    62341 cgattccgct tgaagcgccc atgatgacaa tcactttttc tgataaatca ttcattctat
    62401 tctcctcctg ttttgaaaaa tctcttgctt atactcacta tctcttattt catcttactg
    62461 ttcaaataat ccgttcgaca ggtttgacga ttttggcagg gattcctgca acgatggtat
    62521 tgtctgggac atctttcgta acagttgcat tcgctgcaac aatggcattc tcgccaatcg
    62581 ttacgcctgg cagaatagtt gctcctgctc cgatccaagc atttttcttg atgtggattg
    62641 gctttactga caagcctctt cgttcttttg gatcggttag atggttcacc gtgatgaggt
    62701 tgacacgagg accaatcaat actgaatctt ctaaacagat gccacctaag tcaacgaaca
    62761 tcacttgacg attgataaag acatttttcc ctatactaat atgtttgccg aaatctgtat
    62821 aaaaaggcag cagaacagtc accgtatcat ctactttgtc actaatgata ttttctagcc
    62881 gtttgcggat atctgctggt tcatggtacc cagtattcag cgctgctaaa ggctcttcgt
    62941 tttccgcttg gatctgatgg atcacttcaa agagcggatc cgttactcga atctctttat
    63001 tgacgatttg tttttcgatg aggcttgtca ttttgctccc tccttttatc cttactttat
    63061 aacggacggc ttatgatgtc taatacttat aaaacatact taactattcc ttttaactat
    63121 acttagtggt atgctttttt aaaaacagga gggaaaaaat ggatattcgt atactgacgt
    63181 attttttagc agtagctaga gaaaaaaata tcagccgagc agcaaaatct ctgcatatca
    63241 cgcaacctac tctctcaaaa caactgaaag atttagaaga agaacttggt gtgacacttt
    63301 tcactcgagg ctcccgagag atacagttga cggattctgg tcgatatctt tattcaaaag
    63361 gaaaagaaat cttgtcgttg gtagaaaaaa cggcgaacaa tttaacaaat gacgcaatca
    63421 tctctggaga aatctacatt ggcggaggcg aaactaaagc aatctctttc atttctgata
    63481 gccttcattc gttgatcaag gcgcatcctg atatccagtt tcatttatat agcgggaatg
    63541 cagatgatat catagacaaa ctggatagcg gattacttga ttttggactc gtaatcgaac
    63601 cgacaaacaa acaaaaatac cagtcatttc gactgccagc ccaagatcgt tggggacttt
    63661 tgatacatcg gaatcatcct ttagcaaagc aaacagtcat ccatccgcag gaactgaaaa
    63721 ctgttccttt gatcatttcc cggcaaacgt cagtggacag tcaactagct gaatggctag
    63781 gtttcagcat agatcagtta tcgatcgtcg gaacatataa cttattgtac aatgcttctt
    63841 tgatggtcga taacgggact tttggtgcat tagctctaga tgggatcatc aatacttatg
    63901 ggacggaaaa agtattcatc ccactcgatc cgccattaca agcttcactg aatctcattt
    63961 ggaaaaaaga ccagcctctt tctcctgctg ctcgtaaatt tttagatgtc ctaacggaga
    64021 aatacagtta agtagagttt ttttccttcg ctttcagctt tttaacaacg aataagttaa
    64081 gcaaaagatc caatgattaa ataacataag tttcttaatt tgttccttcc ttttatttct
    64141 gataattcca gtaaattact ataattataa aaaaagaatt gacaaaacat aactgtctat
    64201 ataaaattga ttacgttaac aataattcgt ttcatgtgac tagatcatac taggcatgaa
    64261 aaatctctga ggaacatggt ttccctagga ggtttttcgt gtctattttt gtttggccgc
    64321 aatcaactgg tctagcaaac gtgaaaacca aatcttcaaa aagggtgcgc gcatgaaaat
    64381 caaaaagatc tttaatcaaa atgcagtatt ggtagatgac ggtggacagg aaaaagtagc
    64441 tattggaaaa gggattggat ttgacagaaa aagaaatgat ttgatttttg atcgtgatgt
    64501 cgaacggata tttgtcatgg aaccagaagg tcaaatcaag ttgcaaaatt tacttaatca
    64561 gattgatgaa cgctttttat ttgcagcaga aacgatcatt gatcatgcgg aaactgtttt
    64621 gatggaaaag ttgaatgaac atgtattgat cgctttgacc gaccacattg ctttttctgc
    64681 ggagaacatt aataacggta tcgtcatccg caacaaactt ttaagagaaa tcgaagtgct
    64741 ttacagtgat gaattcagta ttgctcaatg ggcagtagag tatctgacaa aagagcttgg
    64801 gattccatat acttatgacg aagcaggata tatcgcaatc catattcaca gcggacgaag
    64861 cggtcggaac aataaccatc gcagtatccg tgaagtgacg attgtatctg acatcatcca
    64921 tttagtggaa tcagaattgg aaacagatat ccattcagaa ccattttcac tgaattattc
    64981 aagactggtg aatcatttac gactgcttct acaacgaacc catgcgcaac aatatgctgt
    65041 tttagacacg gaaatcgtag aaatggtcaa acgaaaatac ccagaaagct ataagatttc
    65101 taaaaaaatc cgtgtgttgc tgaccaaaga ataccagctc gcgatcacga aagaagaact
    65161 gggttacttg gccatccata tcgagcgttt aagaagcgca atcgtacaaa caaatgttaa
    65221 caatcatgag gaggaaaaaa attgaaagca tatctacaac gaatgggacg ttcccttcaa
    65281 cttccagttg cggtcttacc tgctgctgct ttactagtag ggatcggaaa ctggtgggca
    65341 tcttatagta acgacattgt cgctcatttt ttacaagctg gcggtaacgc agttttaggt
    65401 caattacctt tgctgtttgc cgttgggtta gcactaggga tgtcagtgga caaatcaggt
    65461 gctgctgcct tggcaggttt ggtcgcattt gaagttccag taaatgtttt aaaaactgat
    65521 tcagtagcta ctttattgaa tatcaaacca gaatcagttg atccatcttt tgctcagatc
    65581 agtaacgtat ttattggtat tattgccgga ttgattgcgg cagctcttta taatcgtttc
    65641 cacgaagcaa aattaccgat ggctttatct ttctttagcg gcaagcgtct agtgccaatc
    65701 ttggctgcct ttgtcatgtt gctgatcagt gcagtgctac tgctcgtatg gcctccggtt
    65761 tactctgctt tagtttcttt tgctaaattc attttgagtt taggctgggg tggtgcaggt
    65821 ctttatggct tcttcaaccg tttactgatc cctacaggat tgcatcaagc aatgaactcc
    65881 gtgttctggt ttgacgtagc aggaatcaat gatattggta aatttttggc ttctgaagga
    65941 acaaaaggcg taacaggaat gtatcaagct ggatttttcc caatcatgat gttcgggctt
    66001 ccagcaggag catatgctat ttacagaaat gcacgtccag aaaacaaagc aaaaacagct
    66061 tccttgatgt tagcagcagc ttttgcttca ttctttactg gtgtaacaga accacttgaa
    66121 ttctcattta tgttcgttgc ttggccatta tatgtcttgc atgcaatttt cactgggatt
    66181 tctttagcag tgagtgcatt cttccattgg acagcaggat ttgcctttag tgccggattt
    66241 gtcgattact tcctatcttt gaaaaatccg attgcgaatc agccattgat gttgatcgtc
    66301 caaggattag taacagcagt gatttactac tttggcttta actttgctat caaaaaattc
    66361 catctgatga ctcctggccg cgaggaagca gatctttcag atgacgatac tgcaacgact
    66421 aattcagaca ataaatatgc ggcacaagca agcaaaatct acgctgcatt aggcggagcc
    66481 gacaatgtaa cttcgatcga taactgtact acacgccttc gtttgcaagt caaagataca
    66541 ggcctgatcg atcaaaataa aatcaaagcc actggcgtac ctggtatgaa gatcatcgac
    66601 ggaaaaaatg cgcaagtcat cgtaggtaca gaagtacaat tcgttgcaga tgagatggca
    66661 aaactacatg gtggtgctgc aacacgacct gcacaaacaa acaccgtggt taaaacagaa
    66721 acatttgccg gtggaacgat cgaacaagat atttacgcta tcgcaaatgg gaaactgatt
    66781 ccaatcactg aagtttcaga tgatgtattc gcggaaaaaa tgatgggtga cggctatgct
    66841 gtattaccag aaaatggaga aatcttctct ccaatcgctg gtacgatcac aaacattttc
    66901 ccaacgaaac atgctgtcgg aatccaaaca gatgctggta tcgaagtctt gcttcatatg
    66961 ggcatcaaca ctgttgactt aaaaggcgaa ccattcactt tatatgtaaa agaaggtcaa
    67021 aaagtcgcaa gaggtcaatt gattgcttta gttgatcttg ccgctattca atctgcaggc
    67081 aaaaacacgg atatggtcgt tgtattcaca aatggcgaca aagtccaaag cttagaaatc
    67141 gaacccgcac gtgatgtaaa agcaaatgat aaaatcggct ctgtttccaa caaagcctga
    67201 gccatcgtct tccccagact tttttatcga taaacaaaca ggagctgaga caaaaatctt
    67261 gtctcagctc ctttttccga ataaaaacgg cgttctcctg ccaactccgc ggtaataagt
    67321 ccagccaaac gtaacatatg aagagctatt ttttatttga ctcttcttat tacttgatgc
    67381 agaacagctt ttttacaacc tctgataaac ggtgttcaaa aaccagttta tcggtatgaa
    67441 gtcaaattta tgcaaaaatg tggaaagcca ttttcacaaa atttttcttc atactcaaaa
    67501 ctaatcggct ttttcacaac ctctttatac attattcctg atccaatatt tcttttaatg
    67561 ctgcttcaag agaagttaat ggttttccca atactttttc taaatcatca gaggaaatag
    67621 ctaactgatc ctttttaata tcatgttgga tcgctaaaaa catgtcagct gcttcttgag
    67681 ggaatccggc ttcttttaga ttttcgacaa acgctgcatc acttgcttca ataatttcta
    67741 actttttacc tgtcgcacgc tctaatgcct tggctaattc ttcatagtca gttagattgc
    67801 cggataattc taagatttct ggagagtcac tttctactag tacttttgct gcagcttctg
    67861 catattcacg tttcaatgcc caacctgttt ttccatttcc tgcagcataa acgaattgac
    67921 cagtttttaa agcctgccca atgataggta attcattctc tacataccaa ttattccgta
    67981 aaaatgtatg ggaaatacct gaatccttaa tcattttttc agtgaattgg tgatctggcg
    68041 ctaatacact gatcgagtga tcggcatctg caaaacttgt ataagcgata taagataccc
    68101 cagctgcttg tgctgccttc actacattct tatgttcggc ttgacgattt cctggaacgc
    68161 cagagacaaa aagcaatcga tctattcctt tcaacgcttc tttcattgag tctaaatcat
    68221 cataatcacc aatacgtata ttaaatccag cttctttcag tgcttctcct ttttcttcac
    68281 tacgtactag tacatagatt tcattttgtg gaaccatctt ttttaagaat tccagcgcat
    68341 aaccaccaaa acctccagtt gctcctgtta ctaaataatg catatttgcc actccctatt
    68401 ctttttattt gttaagattt acagctgtac atttataaat tacaccttta tcataaagag
    68461 tgtggtaaga taagtcaagc aaaaaaaatg aaaggatgac aactgatgaa gtattctctg
    68521 caatttagtg atgcaatcca tgtacttgcc tatattgaaa tgtttcaaga tacccgatca
    68581 ctttctagtg agatgattgc cagtagtatc gaaacaaatg cagcaaatgt acgtaaaatc
    68641 atgagtcagc tcaaaaaatc cggtttgatc tttacaaaaa ccggaaaacc gcagccttcc
    68701 cttgccaaaa gtccagatca gatttcttta ctagatgtct atcgcagcat cgaaggtaat
    68761 acacagctta tccaagtcga tccaaaaaca aatccaaatt gcgtcgttgg tgcgaatatc
    68821 caagaagctt tagaagaaag ctacgctcgg ctgcagaaaa aggcagaaga agaaatggct
    68881 aagatcacac ttgatagtct gatacataaa atagccgttt tggaaaaaga aaaaagacca
    68941 gaaaacacag gcctcgtaga gagattttta taattctctc tactgtgcct gcatttttct
    69001 gatcggaatc gtctacataa attcttttat tctccggggg acaaaagcct tgtcatgccc
    69061 ctttttcctc tctaatgctc aggtcaaagc gctgaagtca tgacgtcttg ataaacggtg
    69121 ttcaagtagc tgaccctcgg cattagttct gaaaaatgca aaatatggaa agctatttcc
    69181 catttttcct ctctaatgct caggtcaaag cgctactttc acgacctcta tattatcatg
    69241 gtttattgac taccggtatc cagatttctc ccatatattt cccgttggta actcccattt
    69301 cgactacagc atttggaccc ccaacatagg cgtaatcttc tacttctctt aaaacatgcc
    69361 caaaggccgt attcgtcaaa tcatttctta attcttcagg cgattctgct aaagcgatta
    69421 caacaagata cgttcctttt gggaatggga tgattcgggt agtgtttgga acatcctgac
    69481 tcgtttgaac accgacatag tacatcattt tcccgttata gggttcgttg actgtaaaga
    69541 aataatgatt ttcagcagct tgtttcaatg catctattgt tccattaccg ataacttttt
    69601 gctcaaatgc ttgtttctct gcagccaaac cgacaaaatc cgtattgcta ctcttcaatt
    69661 ccacaccaat acccgtcacc gtaaaatctg gtttttcttc gatcctatac tttgccattt
    69721 ctcccaccct ttctattata ttaagttcat cttacataaa attaaaggga aatgacacga
    69781 ttacgctctt ttttctaggg atatttttat tttttaacta tttttttgct ttcactgcaa
    69841 tccaaatctc actgtaaaca ttactgaaat cctctcccca ttttgtaaaa gagatctcgg
    69901 gagcttcgac taactcataa tctgaagaag gaagccattc tgcatagatt ttcccccaag
    69961 tatcttgtaa agtttgggga aatggccctt tattaggaaa aatggcccaa gtgtgttctt
    70021 ctatagcaag catttctaaa tcagcgtacg gattttcttt tgtcgtagca aagccaatca
    70081 tgtgcatcaa actgcccttt tcttccaagc gggctccatc aaatccataa gaaacgttga
    70141 ttacttgatt tggatataaa tctcctaact tatgcatctc ttccctttga gtctccgtga
    70201 tcgtctgcgc taacttttgt atttcttgat tgactccttc aaatttgaca ggaacttttt
    70261 tcgccacacc tgccacataa aactgttctt tcttttcgat acgaaattcc atcgattttc
    70321 ctcctctgac atcaataaaa aatgtcaagc gtggaaaaga tttttgtatg ccgtatctgc
    70381 tgatttctga aggcagatat cctgaccaat cacgaaaagc tcttgaaaaa ccttcgactg
    70441 tttcatatcc atatctaaac gccgtttctg tcactcttga tccatctagg agatcgcgat
    70501 tagccaaagc caatctacgg tttttgatat agtccattag cgacatccct gcaataaacg
    70561 taaagatgcg ttttaattga tattcagaga ttcctgtatt ttcggctatt attttatagg
    70621 aaagtccttc tgtcaaatgt tcttcgatgt aatcgatcaa ttggttcaga tctttaagca
    70681 tatttatctt cctccttgct tgccttttta tcatattatc aaagaaagtc aattacttga
    70741 tgaatcagga accaatttag tcagatgtag aaaatgttgt atggatctaa agaaaattct
    70801 caagcagcgt ttcgtaacgg ataattggta ttatctgcct atctatgttt tacctttaga
    70861 aaaataaatt ttggggaaga cacattctaa gtaaacaaag cggtaaaaat atcttttatt
    70921 cccttagtta atccgcttac tacctactgt acaaagcaaa ttgttgttca aactgctgat
    70981 cagaacgctg cttggatttt tctttgcgat acatcatacc tcttttatca attggaacaa
    71041 ttatttattt ctaacaaaat cacttcattt ttgtcagata aataaaaaac aaaaaaagtt
    71101 ccatctttaa aacacgctat tcttttactt tttctcacat tctctaagaa aagaaaagga
    71161 gaatga
//
LOCUS       DAZUKC010000003        71130 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000003 DAZUKC010000000
VERSION     DAZUKC010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 71130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.003"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            188..418
                     /locus_tag="ACG80T_000147"
     CDS             188..418
                     /locus_tag="ACG80T_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFS4839836.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            515..1816
                     /gene="murA"
                     /locus_tag="ACG80T_000148"
     CDS             515..1816
                     /gene="murA"
                     /locus_tag="ACG80T_000148"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFS4839837.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1829..2005
                     /locus_tag="ACG80T_000149"
     CDS             1829..2005
                     /locus_tag="ACG80T_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFS4839838.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2140..2436
                     /gene="yidD"
                     /locus_tag="ACG80T_000150"
     CDS             2140..2436
                     /gene="yidD"
                     /locus_tag="ACG80T_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFS4839839.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2621..3088
                     /locus_tag="ACG80T_000151"
     CDS             2621..3088
                     /locus_tag="ACG80T_000151"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4839840.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3173..4057
                     /locus_tag="ACG80T_000152"
     CDS             3173..4057
                     /locus_tag="ACG80T_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS4839841.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4171..4407
                     /locus_tag="ACG80T_000153"
     CDS             4171..4407
                     /locus_tag="ACG80T_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFS4839842.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4495..4575
                     /locus_tag="ACG80T_000154"
     tRNA            4495..4575
                     /locus_tag="ACG80T_000154"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4529..4531,aa:Tyr,seq:gta)
     gene            4582..4653
                     /locus_tag="ACG80T_000155"
     tRNA            4582..4653
                     /locus_tag="ACG80T_000155"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4614..4616,aa:Gln,seq:ttg)
     gene            4778..4948
                     /locus_tag="ACG80T_000156"
     CDS             4778..4948
                     /locus_tag="ACG80T_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4839843.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVRVKSKRP
                     TNHTKKWMAKNR"
     gene            5168..5875
                     /locus_tag="ACG80T_000157"
     CDS             5168..5875
                     /locus_tag="ACG80T_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4839844.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5934..6578)
                     /locus_tag="ACG80T_000158"
     CDS             complement(5934..6578)
                     /locus_tag="ACG80T_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFS4839845.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6704..6991
                     /locus_tag="ACG80T_000159"
     CDS             6704..6991
                     /locus_tag="ACG80T_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4839846.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7099..7419
                     /locus_tag="ACG80T_000160"
     CDS             7099..7419
                     /locus_tag="ACG80T_000160"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4839847.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7500..8814
                     /locus_tag="ACG80T_000161"
                     /pseudo
     CDS             7500..8814
                     /locus_tag="ACG80T_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            8811..9530
                     /locus_tag="ACG80T_000162"
     CDS             8811..9530
                     /locus_tag="ACG80T_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFS4839848.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            9658..10209
                     /gene="raiA"
                     /locus_tag="ACG80T_000163"
     CDS             9658..10209
                     /gene="raiA"
                     /locus_tag="ACG80T_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFS4839849.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10431..12965
                     /gene="secA"
                     /locus_tag="ACG80T_000164"
     CDS             10431..12965
                     /gene="secA"
                     /locus_tag="ACG80T_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFS4839850.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13091..14192
                     /gene="prfB"
                     /locus_tag="ACG80T_000165"
     CDS             join(13091..13162,13164..14192)
                     /gene="prfB"
                     /locus_tag="ACG80T_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFS4839851.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14297..14983
                     /gene="ftsE"
                     /locus_tag="ACG80T_000166"
     CDS             14297..14983
                     /gene="ftsE"
                     /locus_tag="ACG80T_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFS4839852.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14976..15860
                     /gene="ftsX"
                     /locus_tag="ACG80T_000167"
     CDS             14976..15860
                     /gene="ftsX"
                     /locus_tag="ACG80T_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFS4839853.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            16012..17460
                     /gene="cls"
                     /locus_tag="ACG80T_000168"
     CDS             16012..17460
                     /gene="cls"
                     /locus_tag="ACG80T_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFS4839854.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17797..18654
                     /locus_tag="ACG80T_000169"
     CDS             17797..18654
                     /locus_tag="ACG80T_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFS4839855.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18769..19689
                     /gene="pstC"
                     /locus_tag="ACG80T_000170"
     CDS             18769..19689
                     /gene="pstC"
                     /locus_tag="ACG80T_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFS4839856.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19689..20573
                     /gene="pstA"
                     /locus_tag="ACG80T_000171"
     CDS             19689..20573
                     /gene="pstA"
                     /locus_tag="ACG80T_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFS4839857.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20588..21394
                     /gene="pstB"
                     /locus_tag="ACG80T_000172"
     CDS             20588..21394
                     /gene="pstB"
                     /locus_tag="ACG80T_000172"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFS4839858.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21410..22168
                     /gene="pstB"
                     /locus_tag="ACG80T_000173"
     CDS             21410..22168
                     /gene="pstB"
                     /locus_tag="ACG80T_000173"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFS4839859.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22181..22858
                     /gene="phoU"
                     /locus_tag="ACG80T_000174"
     CDS             22181..22858
                     /gene="phoU"
                     /locus_tag="ACG80T_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFS4839860.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23435..24127
                     /locus_tag="ACG80T_000175"
     CDS             23435..24127
                     /locus_tag="ACG80T_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFS4839861.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            24124..24891
                     /locus_tag="ACG80T_000176"
     CDS             24124..24891
                     /locus_tag="ACG80T_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFS4839862.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24991..26559
                     /gene="liaX"
                     /locus_tag="ACG80T_000177"
     CDS             24991..26559
                     /gene="liaX"
                     /locus_tag="ACG80T_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFS4839863.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26856..27176
                     /locus_tag="ACG80T_000178"
     CDS             26856..27176
                     /locus_tag="ACG80T_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFS4839864.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            27176..27526
                     /locus_tag="ACG80T_000179"
     CDS             27176..27526
                     /locus_tag="ACG80T_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFS4839865.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27693..28631
                     /gene="hprK"
                     /locus_tag="ACG80T_000180"
     CDS             27693..28631
                     /gene="hprK"
                     /locus_tag="ACG80T_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFS4839866.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28646..29476
                     /gene="lgt"
                     /locus_tag="ACG80T_000181"
     CDS             28646..29476
                     /gene="lgt"
                     /locus_tag="ACG80T_000181"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFS4839867.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29520..30545
                     /locus_tag="ACG80T_000182"
     CDS             29520..30545
                     /locus_tag="ACG80T_000182"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFS4839868.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30560..31498
                     /gene="galU"
                     /locus_tag="ACG80T_000183"
     CDS             30560..31498
                     /gene="galU"
                     /locus_tag="ACG80T_000183"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFS4839869.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            31623..32063
                     /locus_tag="ACG80T_000184"
     CDS             31623..32063
                     /locus_tag="ACG80T_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFS4839870.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            32066..32668
                     /locus_tag="ACG80T_000185"
     CDS             32066..32668
                     /locus_tag="ACG80T_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFS4839871.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(32727..33833)
                     /locus_tag="ACG80T_000186"
     CDS             complement(32727..33833)
                     /locus_tag="ACG80T_000186"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFS4839872.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            34043..35044
                     /gene="ccpA"
                     /locus_tag="ACG80T_000187"
     CDS             34043..35044
                     /gene="ccpA"
                     /locus_tag="ACG80T_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFS4839873.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(35381..37726)
                     /locus_tag="ACG80T_000188"
     CDS             complement(35381..37726)
                     /locus_tag="ACG80T_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFS4839874.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     RIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            38005..38907
                     /locus_tag="ACG80T_000189"
     CDS             38005..38907
                     /locus_tag="ACG80T_000189"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFS4839875.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            39120..40895
                     /locus_tag="ACG80T_000190"
     CDS             39120..40895
                     /locus_tag="ACG80T_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFS4839876.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(40961..42547)
                     /locus_tag="ACG80T_000191"
     CDS             complement(40961..42547)
                     /locus_tag="ACG80T_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFS4839877.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            42798..43022
                     /locus_tag="ACG80T_000192"
     CDS             42798..43022
                     /locus_tag="ACG80T_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFS4839878.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            43164..44915
                     /locus_tag="ACG80T_000193"
     CDS             43164..44915
                     /locus_tag="ACG80T_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFS4839879.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            44915..46699
                     /locus_tag="ACG80T_000194"
     CDS             44915..46699
                     /locus_tag="ACG80T_000194"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4839880.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            47025..47876
                     /gene="licT"
                     /locus_tag="ACG80T_000195"
     CDS             47025..47876
                     /gene="licT"
                     /locus_tag="ACG80T_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFS4839881.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            47969..49855
                     /locus_tag="ACG80T_000196"
                     /pseudo
     CDS             47969..49855
                     /locus_tag="ACG80T_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
     gene            complement(49895..50797)
                     /locus_tag="ACG80T_000197"
     CDS             complement(49895..50797)
                     /locus_tag="ACG80T_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4839882.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            51028..52431
                     /locus_tag="ACG80T_000198"
     CDS             51028..52431
                     /locus_tag="ACG80T_000198"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFS4839883.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            52431..53864
                     /locus_tag="ACG80T_000199"
     CDS             52431..53864
                     /locus_tag="ACG80T_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFS4839884.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            54009..54935
                     /gene="trxB"
                     /locus_tag="ACG80T_000200"
     CDS             54009..54935
                     /gene="trxB"
                     /locus_tag="ACG80T_000200"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFS4839885.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            55149..56876
                     /locus_tag="ACG80T_000201"
     CDS             55149..56876
                     /locus_tag="ACG80T_000201"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFS4839886.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            56891..57241
                     /locus_tag="ACG80T_000202"
     CDS             56891..57241
                     /locus_tag="ACG80T_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFS4839887.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(57333..58193)
                     /locus_tag="ACG80T_000203"
     CDS             complement(57333..58193)
                     /locus_tag="ACG80T_000203"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFS4839888.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            58285..58713
                     /locus_tag="ACG80T_000204"
     CDS             58285..58713
                     /locus_tag="ACG80T_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFS4839889.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            58876..59052
                     /gene="rpsU"
                     /locus_tag="ACG80T_000205"
     CDS             58876..59052
                     /gene="rpsU"
                     /locus_tag="ACG80T_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFS4839890.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            59079..59525
                     /locus_tag="ACG80T_000206"
     CDS             59079..59525
                     /locus_tag="ACG80T_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFS4839891.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(59679..61058)
                     /gene="brnQ"
                     /locus_tag="ACG80T_000207"
     CDS             complement(59679..61058)
                     /gene="brnQ"
                     /locus_tag="ACG80T_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFS4839892.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            61764..62735
                     /locus_tag="ACG80T_000208"
     CDS             61764..62735
                     /locus_tag="ACG80T_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFS4839893.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            62759..64951
                     /locus_tag="ACG80T_000209"
     CDS             62759..64951
                     /locus_tag="ACG80T_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFS4839894.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            64968..65444
                     /gene="ybeY"
                     /locus_tag="ACG80T_000210"
     CDS             64968..65444
                     /gene="ybeY"
                     /locus_tag="ACG80T_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFS4839895.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            65428..65823
                     /locus_tag="ACG80T_000211"
     CDS             65428..65823
                     /locus_tag="ACG80T_000211"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS4839896.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            65838..66737
                     /gene="era"
                     /locus_tag="ACG80T_000212"
     CDS             65838..66737
                     /gene="era"
                     /locus_tag="ACG80T_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFS4839897.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFTQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            66880..67692
                     /gene="recO"
                     /locus_tag="ACG80T_000213"
     CDS             66880..67692
                     /gene="recO"
                     /locus_tag="ACG80T_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFS4839898.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            68076..68993
                     /gene="glyQ"
                     /locus_tag="ACG80T_000214"
     CDS             68076..68993
                     /gene="glyQ"
                     /locus_tag="ACG80T_000214"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFS4839899.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            68995..71070
                     /gene="glyS"
                     /locus_tag="ACG80T_000215"
     CDS             68995..71070
                     /gene="glyS"
                     /locus_tag="ACG80T_000215"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFS4839900.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGKASRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        23299 a        12454 c        15199 g        20178 t
ORIGIN      
        1 tttggaataa gggactgcga caagactttt gtctcagtcc ctttttttgc atatttttag
       61 catttatttc ctaaaaaatg taaggggatt cctatatttt taagtatact tgtttttcct
      121 tttatacatt ttatggtatt atcattttat tagttattat aaaacaatcg ttagaaagga
      181 cgtcattatg caagtatacg gaattgatgc ggtgcttcga atcgtgagtc atttggcgtt
      241 tatctactta gccttctggt ctttacgttc gttacgcatt gaaacttttt tcagaccaat
      301 gcataatacg caaattagaa tggcaatcgt acttttttca atttttttag ggtacactgc
      361 aagtaatttt ttcttagaag ttatcgcttt atgccgaaat cttttcgtta gcctgtaaat
      421 tttaaaaaaa ggtactattt taaaagcaag gatgttataa tagaaaagat tttgagtaag
      481 tgtatgctca ttggaatttt ttggagggaa caacatggaa gagatcatcg taagaggtgg
      541 caatcaatta aatggaacag taagaattga aggagctaaa aatgctgttt tgcctatttt
      601 agctgctagt ttgttagcag aagaaggtat tacgacatta gacaacgttc cgattctttc
      661 tgatgtcttc actatgaatc aagtgattcg tcacttaaac gtagatgtag attttgatga
      721 acaaaagaat caagtaacaa tcgacgcttc aagacaatta gaaatcgaag cgccttatga
      781 gtatgttagt caaatgcgtg cttcaatcgt tgtgatgggt ccgctacttg ccagaaatgg
      841 acatgcaaaa gtagctatgc ctggtggatg tgcgattggt aaacgaccaa tcgatttgca
      901 tttaaaagga tttcaagctt taggagcaaa aattattcaa aaaaacggct atatcgaagc
      961 aattgcggat gaattgatcg gtaacacgat ttatttagat ttccctagtg taggtgctac
     1021 tcaaaatatc atgatggctg ccgtaaaagc aaaaggaaca accatcattg aaaacgtagc
     1081 tcgcgaacca gaaatcgttg atttagcaaa cattttgaat aaaatgggcg ctcaagtcta
     1141 tggagctggt actgaaacaa tgcgtattga aggcgtggat catttacatg cagtcaacca
     1201 ctcaatcgta caagatcgta tcgaagcagg tacctttatg gttgctgctg caatgacaca
     1261 aggcaatgtg ctgattgcag atgctatctc tgaacataac cgcccattga tttctaaatt
     1321 gatcgaaatg ggtgcagaaa tcattgaaga agaaggcgga gtccgtgtca tcggaccaaa
     1381 acatatctta cctacagatg taaaaacgat gcctcatcct ggtttcccta cagatatgca
     1441 agctcaaatg acagctatcc aattagttgc agaaggtaca agtgtagtga ctgaaacagt
     1501 gtttgaaaat cgtttccaac atttagaaga gatgcgccgt atgaacgctc atgtcaaaat
     1561 cgacggaaat gtagcaatca tggatggaaa ccatgaattg caaggtgcag aagtatatgc
     1621 aacagacttg cgggcagcag cagcattagt tttagctggc ttgaaagcaa atggaatcac
     1681 acgtgtgcgt aacttgaatt atctagaccg tggatattat aatttccata tcaaactaca
     1741 gcaattaggt gcagacgttg aacgtgtaga catggatcaa acttctgctg aaaaaacagc
     1801 acaaacgatt gcttaaggaa gtggctgaat gagtaaccaa cgatatatct tattaacttt
     1861 gataaagatt ttaatggtga tcttattgct tatccttctt tttgtggccg gaacgatgat
     1921 cggatatggt gtcatcggtg gcggcaatcc atttaaagta tttcagccaa gtttgtggat
     1981 tcatattcga gacttttttc attgataaat aagaatcaca gtggttctag tcgatatgaa
     2041 gtgagccatg aagcagaaca tgataaaaga aaacagaagg gaacgttgac gttagtcgcg
     2101 atcccttttt ttagtaaaag aaaagaagga atctaattga tgaagaagat attgatttat
     2161 ttagttagag gatatcagcg ctttatttcg cctgcatttc cacctagctg ccgttattac
     2221 ccaacctgtt ccaattatat gataaaagca attcaaaccc atggcgcgct aaaagggacg
     2281 gtaatgggga ctgcacggat cttacgctgt catccttttg tcaaaggcgg tatcgattat
     2341 gtaccattga cattccgatt aacgcgaaat aagaaagata aaaaagaacc agattataaa
     2401 aaggttgtga aaaatacggg tagcactgag cgataaagaa acaatttgga aaatagcttc
     2461 ttatattttg caaaattgtg agttattgcc gaagtgttgg attttgaaca ccgtcaaaaa
     2521 aaaggttgtg aaaaatacgg ctagcactga gcgataaaga aaaagaggaa atttattgtc
     2581 aaagtaacaa ctccgcaact acttatatgg gggacaaaaa atgatcgaag aactaaacaa
     2641 gaaagattta ccatggtcct tgttattaga tgcagatcct gataaagaaa aagttcaagt
     2701 atatgtttcc agagggagtg gctggatatg gaaagaaaaa gaaaaaacaa taggtgtact
     2761 gatctatgtg gcaagggaaa aagaatacga aatcgtcaat gtagcagtaa ccccaacgta
     2821 tcaaggaaag gggattggcg gaaaattact tgagacagct tttcagaagc tttcacaact
     2881 cgcttccagt caaactcaga tcatcatacg aaccggcagt actagcagtg cggcgttaca
     2941 tctctatcaa aaaatgggct atgtggagat tggtcgagtc aaagactatt ttatccatca
     3001 ttactctgag cccatttttg aaaatggaga tcaattaagg gatcaagtga ttttagcaat
     3061 tcacttacct aaaaatagct ttaactaata aaatttcaat tcaagaaaat gctttcaaat
     3121 gctaaataga tacattacaa tagctttagc aaacaaaaag gagaatattt ttatgggaat
     3181 attagttttt gattttggtg gatcggctgt caaatatggt tgttgggatg gcaaagagat
     3241 taaaggtaag ggacaatttg caacgcctga atcttgggaa gaaatgaaag cgcaattatt
     3301 tcaagtttat aaaaaaatac tcgtttcttt tgaagtcgaa ggaattggaa tcagctctcc
     3361 tggagtagtg aataatgaga aacaggtgat tgagggaatc agtgcgattc cttatattca
     3421 tggatttaat attttccgag atttggaaac tctgtttcaa cttccagtaa cgattgaaaa
     3481 tgacgccaac tgtgccggaa tggctgaatt ttatgaaggt gctgcccgcg attatcaaga
     3541 tgttgctttt gttgtggcag gaactgggat tggcggggcg ttgttcacga aaggacaaat
     3601 caataaaggg gcacatcttt acggtggaga atttgggttg atgttcttaa atggcgataa
     3661 gacattcagt aaacttggta cagcggtaca gatggcttgg cgttattgtg aacgcaaagg
     3721 tctcgataaa aatgcttata ccggaaaaga tgtctttgaa ttagcagaaa ctggagatgc
     3781 aatagcaaaa gaagaagttg agagttttta tacgtatctt acaaagggat tattcagtat
     3841 tcaattttcg tttgatccag aagtgatcgt attaggcggc ggggtttctg caaaagaagg
     3901 gctcattgat gagatcaaaa gccgaatgaa gaagctgaca gaacaattcg atctgcatga
     3961 tttcgaaccg cagattttat tatgtgaata cagaaacgat gctaatcttg taggtgcagc
     4021 agcaaactat ctcgctgtac gtcaagggga gaattaagag ctttagagaa ggtgcagctt
     4081 gcataaaaga agtgaatgaa atgaatagga gatttttacc taaggaatgt tttatttgct
     4141 gcagtatgat aaaatatctg agaggtgaaa atgatgtcaa aaaaaaataa agatattgaa
     4201 gtgcgtatag aagaagtgaa aaaagatatc aaaggtaaga attatgaagt cactcagtta
     4261 tttataggga aaaaaatgat tggtgagatc ttagcctatg gaccaaaaga atatgaaatt
     4321 ttctttggtg aagaagattt tggaaaagaa aaaagtcttg aaaatgcgat agaaacagtg
     4381 attcgacact ggaatctgca cgaataaaaa aatatcaaaa aaactttcaa taaagggttg
     4441 caaaaatctg gaaaattagg tattataact aagtcaggtt gatacaaacc tttcggagga
     4501 gtagcgaagt ggctaaacgc gacggactgt aaatccgttc cttagggttc agtggttcga
     4561 atccactctc ctccatttca ttggggtata gccaagcggt aaggcaaggg actttgactc
     4621 cctcatgcgt tggttcgaat ccagctaccc cagtatacca caatcgtaag ctctcgttag
     4681 ttcgagagct cttttttttg ctttgctacc ttgttccttg aagattctgc tgatttaaga
     4741 tagagttaag ttaactacaa gaattggagg gatccacatg aaatggaaag aaaaagtaga
     4801 cgaagcagct gaaaagttat atgatttggt caaatcagaa aaatacaata ttgaagtgaa
     4861 tattccgaaa aaaggcggaa aagcggtccg ggtaaaatca aaacgcccaa cgaatcatac
     4921 aaaaaaatgg atggctaaaa accgctaata ataacaaaag atccataagt cggcattttc
     4981 atcgatttta gggtcttttg ttattttcta ggttggacat atctaaaaaa ttgtttctga
     5041 caataggagt aggaagaacg atgtgtgtct aaatcctctg ttgatggata gaaaccttta
     5101 tctttttcag ttgtgaatca ggtatatacc agttataata gaagaaacga aaggacgggt
     5161 gtacgttatg tcgaatcaat tacctgtata tatacagatt catgaccaat taaaaaatga
     5221 aatcgaacaa ggtatatgga aagtcggaga ccgtcttcct tcagaaagag aattagcagt
     5281 gaaattcaat gtcagccgta tgacgttgag acaagcaatt caaaacttgg cagatgaagg
     5341 aatcttagaa agaaagatcg gttcgggtac atatgttgca cgagaaaaag tacaagaaaa
     5401 aatgtctggt gcaacaagtt ttacagaaat tatggaatca caaaatcgag taccgtctag
     5461 tcggacaatc tcttatttct tgacttcgcc aagctcaagt gaaatggaaa aactaaactt
     5521 gagtaaagat gacacgatct tgcgaatgga acgtattcgt ttcgcagatg atgtccctat
     5581 ttgttttgaa gttgccagtg tgccacaaaa gctgatcgat ggctacagca aagcagagat
     5641 tactgaatcg ttgtatcgca cgctagaaga aaaaggcggg aaaaagatcg gtccagcgaa
     5701 tcaaacgatt actgctgtgt tggcttctga gaaaattgct gactatttag aggtaaaacg
     5761 aggagatgca gttcttcgtt tgcgccagat ttcttatttt gaagacggta cgccgtttga
     5821 gtatgtgcgt acgcaatacg taggaaatcg ttttgagttt tatttggaaa aataaaaaac
     5881 aggtgatccc tgattccgta ttagaaataa aagagaatca gaatcacctg tttttatata
     5941 tgatcagtta ccagttgctc gtgataagaa acaggaccct cttcaaattg gacttgtccg
     6001 ttcagcagct caatgatgga ttctttaaag tggtcagcac ttttttcatc tacagcacag
     6061 atgacgctaa cttgatcagt atattggatt tcttttataa aaaaatgttg gagttcaaga
     6121 taattctgta attttcctag ttgcggatac gtgatcatca cagagatctc ctgttgcaag
     6181 gtaccttcta cgattccaat gtcagacaat gcttgagaga ccgcatgagt gtatgctcgt
     6241 attaatccgc ctgtgccgag tttcgttcct ccaaaatatc gagtgactac tgcagctaca
     6301 ttgatcagtt gatttttttt gaggacttcc aacatcggta tgcccgccgt tccacttggt
     6361 tctccatcat cgctgcttcg ctggatttca tttttttctc caatgatgaa ggcgctgcag
     6421 ttatggttcg ctttccaatg ttcttttttt atttcttgaa tgaaactttt tgcttcgtcc
     6481 tctgattcta ttcgtctaag agaacagata aagcgtgatt ttttgatctc gatctcactt
     6541 tgtccatttt cttttattgt acgatagcgc tcgatcatct tgtcacctct tttttcattt
     6601 tctttattca gtataaataa agttctaggt tgtttcaaca ctgtaacggt tgcgcaattg
     6661 ctgtagtatg cgataataaa gacaaaggca ggtggatctg gggatgcagg aacgaatgaa
     6721 aaagtacgat gcaattacgc attatttgaa gaacaatggc ggctctcaag taacgttgac
     6781 ctttacccaa tttgacgaac tgctgtttcc cagtaacgga cttcccaaaa cagcaaggga
     6841 aagtacagat tggtgggcaa atgattataa acaccctgaa aaaggtgcct atggctggat
     6901 aaatgcaggc tatgaagtag ttgtcatcaa tttggacaaa gaatatgtcg tgttcaataa
     6961 attagtgaaa tctagttggc tatttgatta aataaaccgc gtttagaaaa cgttgacaaa
     7021 tttctttctt tttatataat agagtaacag acgcgaagaa aacgtttgcg tagtttgttt
     7081 taaaaaggag agaaaagtat ggctaaaaaa acaatcatgc ttgtatgttc ggctggtatg
     7141 agcacaagtt tactagtaac aaaaatgcaa aaagctgctg aagcaaaagg aatcgactct
     7201 gatattttcg ctgtgtccgc ttctgacgca gataacaatc ttgcaaataa agacgtggat
     7261 gtgttgttat taggcccaca agttcgcttt atgaaagctg attttgagaa acgtctggaa
     7321 ccaaaaggca ttcctttaga tgtcatcaat atggctgatt atggtatgat gaatggcgaa
     7381 aaagtgttag atcaagcaat cacgctcata ggtgaataaa tagaacagta taactatcga
     7441 acaagcgatc caaaaaatag atcgcttgtt ttttcgtata tttttttagg gcgaataata
     7501 tgaatcattt ttggggtaga aggcttctga cgagagatat taaagcaata gactgtgagg
     7561 aggtaaattt accaaattat gagaagatcg cagcagtgga acgtttgagc aactggattc
     7621 tttgtcatcg ctgtggaatg agaacgtccg tcattgatgg acagcttgca gattacggtt
     7681 atttttgtac acattgtcta tatttagggc gatgcgacac tcaacaagat ttatatttgt
     7741 ttgatcagcc tgaagcaaaa cctcgagaag tcgtcttttt tttggacagg acagcttact
     7801 gaaaaacaaa cggaaatctc aaagagaata ctttgccatt cagaaaaaag acatcatctt
     7861 atttgggctg taacaggtgc agggaaaaca gagatgcttt atcctatttt agtggaaact
     7921 ttaaaagctg gaggaagagt agcgatttgt acaccgcgag tcgatgtttg taatgaattg
     7981 tttttacgct atcgttatgt ttttccagaa gaaaaaatca cgttgctcca tgggaataca
     8041 gcaacggctt atacctttac ttcttttgtt atctgtacga ttcatcagct ataccgtttt
     8101 tatcggtcct ttgatttaat agtgatcgat gagatcgatg cgtttcctta taacggggat
     8161 gcaggattag ctcatgctgt gcaaacagcg attaaaccag acggacgatt catctactta
     8221 agtgccacac ctgataaaaa gttactaaag gagatcaagc agacgtttga catccacaaa
     8281 cttgctttac gctttcatcg gcgtttgctt cctgaacccg tcttggtttt catgaataat
     8341 tggcgacaaa aatgcaagaa caagaagaag ataaaatctt tggtgcggat cattcatcaa
     8401 ttaattaaag aaaaccatat ccttttattt tgtccaagta tcacgatgat gcatcgtttg
     8461 aaagccacac ttcaagaggc tcttccgaga tatgagatca cagatgtttc ttcaagagac
     8521 aatcagcggg cggaaaaagt ccagaaaatg cgagaagcaa aatatgacat actgttaacc
     8581 acgatgatcc ttgaacgtgg agtcacattt gaaagaatat ccgttatcgt tttaggagcg
     8641 gatcatccgg tttttactaa atcttctttg gtacaaatcg caggaagagc cgatcgtaaa
     8701 gggccttata cgaacagtca agtatatttt ttctatgaag aaaaaactgc agctattcga
     8761 aaagcttgtc aggaaatcaa agaaatgaat gaagaaggga agaagatact gtgaggtgca
     8821 gttgctgcag taaagatatc gtccaaaatt taacattcag cgagattatt ggtgtttcga
     8881 aatacagatt acctcagact tgttcagttt gtcgcgcaca atttcattta ttggaaacaa
     8941 gaggctgttc tggctgtcaa aaaccaatcc atttcaatct agagagtacg aaaaagaaag
     9001 taaattctat ccccgtttac tgcagtgact gtcaagaatg gaagaagaaa tatccgacct
     9061 atgattttca gcaccgtgcc ttttttgtgt acgatgaaaa tatgaaggcc tggctcaatc
     9121 aatacaagtt tatgggagat atccgactag caggcacctt tgcatataaa tggacgcaga
     9181 taaaaaaaga atacccgaca tacatctatt gtccaatacc tttatctgaa aaaaggcgaa
     9241 cagaaagagg attcaatcaa gtaagcgaga tgttgaaagc agcaagaatt ccttttcaga
     9301 tgctactcaa aagagagaag catctgctgg ctcaagcaaa aaacacgcga aaggaaagat
     9361 tggagatgcc tcaacctttt gaacttgcag tcaccttttc taagatacaa aataaaaaaa
     9421 tattgattat tgatgatgtc tatacaacag gacagactct tttccacgca gcagattgtt
     9481 tgcagatcgc acagccgaaa atcatccgaa ctatttcttt ggcgagatga attagatgtg
     9541 gaaatttgat agcgatttaa tttgcaaata acctagagtt cgatataata gaattaagaa
     9601 gagggaaaga gtggtccttc tccttcttga ttgtggtaga cgaaaggggt aatttttatg
     9661 tttagatata atgtacgcgg cgaaaatatc gaagtaactg aagcgattcg cgactacgtt
     9721 gagaaaaaag taggcaaact agaacgctat ttcagtgacg cacccgatgc aacggcgcat
     9781 gtgaacttaa aagtttatac agaaaaaaca gcaaaagtgg aagtaacgat cccattacct
     9841 tatcttgtat tacgtgccga agaaacttct ccagatttat acgcaagtat cgatttggtt
     9901 gttgacaaat tggaacgcca aatccgcaaa ttcaaaacaa aaattaatcg taaatcccgt
     9961 gaaacgggca tgaatactgc aaatgctgct gctttattca atgaagagga cgaaaacgaa
    10021 aatgattctg aattagatat cgtacgtacg aaacgccttt cattgaaacc aatggatagt
    10081 gaagaagcag tcttgcaaat gaacatgctg ggacataact tctttatttt tgaagatgca
    10141 gaaacaaatg gtacaagtat cgtttaccgc cgtaaagatg gaaaatacgg actgattgaa
    10201 acagactgat cagccttact taaataacaa cgaaaacttg cagcagctac tgacagctgc
    10261 cgcaagtttt tttgagtttc ctcctctttt agatgaaata tgaataaatt atgaagttct
    10321 tactttaata cgcttaagat atttcaatta tctataacta atgataaaat gaaaagtaaa
    10381 gtctacatag tctggataat acacagaagg aaaaggagat tacacaacgc atggccaatt
    10441 tgattcgttc acttatcgaa aacgataaga aagaactaaa acgtttagat agcattgcaa
    10501 aaaaagttga agcatacgct gaccaaatgg cagcattaac tgatgaacaa ttacaggcaa
    10561 aaactgctga attcaagggc agataccaaa aaggagaaac attagatcaa ttacttccgg
    10621 aagcttttgc agtcgtccga gaagcagcaa aacgtgtatt aggtttatat ccataccacg
    10681 tacaattgat gggtggaatc gtgcttcacg atggaaatat ccctgaaatg cgtactggtg
    10741 aaggtaaaac cttgaccgca acgatgccag tatatctaaa tgctctgact ggcgaagggg
    10801 tacacgtagt taccgtcaat gaatatttag caacccgtga ctcgacggaa atgggcgaac
    10861 tttataattt cttaggatta acagtggggt tgaatatcaa ctcaaaatct tcggatgaaa
    10921 aacgcgaggc ttataattgt gatatcactt atagtacgaa taatgaatta ggatttgact
    10981 acttgagaga caatatggtc gtttaccgcc atcaaatggt acaacgtccg ctgaattatg
    11041 ccattgtcga tgaagttgac tcaatcttga ttgatgaagc gagaacgccg ctgatcattt
    11101 cagggcaagc agaaaaatcc acagctttat atacaagagc agacaacttc gtgaaacgtt
    11161 tgaaagaaga agaagattat aaaatcgatg tacaatcaaa aacaatcagt ttaaccgaag
    11221 cagggatcga aaaagcagaa gaaacattcg gtttggataa cttatacgac attgaaaata
    11281 ctgcattgac ccatcacttg gatcaagcat tgagagccaa ctatatcatg cttttagata
    11341 tcgattatgt tgtccaagac ggaaaagtac tgattgttga ccagttcact ggtcggatca
    11401 tggatggacg ccgttattca gatggacttc accaagcaat cgaagctaaa gaaggcgtag
    11461 agatcgaaga cgaaacgaaa accatggcga cgatcacttt ccagaactat ttccgtatgt
    11521 acaagaaact tgcgggaatg actggtacag ctaaaacaga agaagaagaa ttcagagaaa
    11581 tttataacat ccaagtatac cagattccga caaatcgtcc ggttatccgt gatgatcgtc
    11641 cagatttgct ttacccaaca ttacaaagca agttccacgc tgtcgtgcaa gatatcaaag
    11701 atcgctaccg caaaggtcaa cctgtgttag tcggaactgt tgcggttgaa acatctgagt
    11761 tattatcaga tatgctgaat caagaacgta ttcctcatga agtgctgaat gcgaaaaatc
    11821 actttaaaga agcagagatc atcatgaatg ctggtcaaaa aggtgctgtg acgattgcta
    11881 ccaacatggc tggtcgggga acagatatca agttaggact tggggtaaga gaacttggtg
    11941 gtctagcagt tatcgggaca gaacgacatg aatctcgtcg tatcgataac cagcttcgag
    12001 gacgtgcagg acgtcaagga gaccctggta tgtcacagtt ctacttgtca ttggaagatg
    12061 atttgatgaa acgattcggt tcagaacgaa tcaaatcttt cttagatcgg atgaagatcg
    12121 gtgaggaaga tgctgttatc caaagcaaga tgctgacgaa acaagtagaa tctgcacaaa
    12181 aacgtgtaga aggaaataac tatgacacac gtaaaaacgt cttacaatac gatgatgtga
    12241 tgcgtgaaca acgtgaagta atctataaac agcgacaaga agtaatcatg gaagaaaaaa
    12301 gcttatccaa gccactaatg aacatggtca aacggacaat cagtcgtgta gtagatgcgc
    12361 atacacaatt agagaaagaa aattggaatt tggaaagtat catcgatttt gccggcagta
    12421 ctcttgtcca tgaagatagt attactcttg cagatatcga aggaaaaaca gctcaggaga
    12481 tcaaagagta tctaaacgaa cgagcacaag aagtatttaa taccaaagcg gctcaactaa
    12541 atggtccaga gcaactgttg gaatttgaaa aagtcgtgat cttgcgtgtt gtggactcta
    12601 aatggacaga ccatattgat gcaatggatc aattacgtca gtcaatcggc ttacgtgctt
    12661 atggacagaa caatccatta gtcgaatatc aaacagaagg ctataagatg tttgaagata
    12721 tggtaggcgc catcgagtat gaagtcactc gaatctttat gaaagctgag atccgtcaaa
    12781 acgtacagcg tgaacaggtt tctcaaggaa cagcttctca ctcagaagac ggagatgcca
    12841 aagaaaatag taatactagc gcgaaaaaac agccaatcca tgtgaacaag gtcggaagaa
    12901 atgatccttg tccttgtgga agcgggaaaa aatataagaa ctgtcatgga aaaaatgcaa
    12961 actaatttct gacagcagga ttggtgagac aaattctttt tttaagaatt cgtctcacct
    13021 tctttctgtt atttgctgat tctgttagac tagatataat gaaaaaagta tagtgaaaga
    13081 aggaaaaaac atggaaatat ccgaaattcg aaatctcctg acagaaatgc aggaaaaggt
    13141 cacaagcttc aggaggtctc tttgacttag accagctgga agaagacatt gcggaagcag
    13201 aagcaaaaat ggctgaacct ggtttttggg atgatagcga agctgctcaa aaagtgatca
    13261 gtgaaaacaa tgcaaataaa gaaacttatg atcaattcca ttctcttgct gaagaactgg
    13321 aagaaatcga attgatggtg gagatgttgc aggaagaacc tgatgcagcc atgcaagaag
    13381 aagcagaaac acgtatcaat gcgctagatg aaaaaatgaa gacatatgaa ttatctatgt
    13441 tgttagatgg tccttatgat cgaaacaacg ccatcgttga attgcatcca ggggctggcg
    13501 gaacggagtc ccaagattgg ggcagtatgc ttttacgtat gtatacacgc tgggcagagc
    13561 agcatgggta tcaagtagag acactagatt atcaagcagg tgatgaagca ggaatcaaga
    13621 gtgtgaccct actgatcaaa ggacacaacg catttggtta tctgaaatca gaaaaaggtg
    13681 ttcatcgcct tgtacgtatc tcgccttttg actcagcgaa acgacgtcat acttcttttt
    13741 gttcggtaga tgtgatgcca gagcttgatg aaacgatcga tatagaaatc aatccagatg
    13801 atttgaaaat cgatacgtac cgagcaagtg gtgcaggtgg tcagcacatc aataaaacag
    13861 aatctgctgt acggatcact cacttgccta caggaacagt agtagctagt caggcacaac
    13921 gttcacaatt gaaaaaccgt gagcaagcga tggggatgtt aaaagctaag ctttatcaat
    13981 tagaagtgga aaagaaagaa caagaagcag ctgcacttcg aggagaacaa aaagaaatcg
    14041 gctggggttc acagatccgt tcttatgttt tccatccata ttctatggta aaagaccatc
    14101 ggacaaacgt agagacagga aatgtccaag cagttatgga cggtgacctt gatttattta
    14161 ttgacggcta tttaaaaatg catctacaat aagaacccat tgttaaattg aaataaaatt
    14221 gtaagatttt gcaagcatgt tgaaacattg ccatgctata atagctaaag actgaaaaag
    14281 atatggagag aacgccatga ttgaaatgaa ggatgtaatg aagaagtact caaatggtac
    14341 gaccgctatc cgcaatctct caattgtcat cgaacaagga gagtttgtct atgtggttgg
    14401 tccgtctgga gccggtaaat ctacattcat caaattgatg tatcgagaag aaaaagcttc
    14461 aaaaggaact ttgaacgtcg caggtcatga tctaatagca atcaaaaacc gcgaagttcc
    14521 ttatctacgc agagaaatcg gcgtggtgtt ccaagactat aaattattac caagaaaaac
    14581 agtatatgaa aatgttgcgt acgctatgca agttatcggc agaaaacctc gggatatcaa
    14641 aaaacgagta atggaagtgc tggaccttgt tggtttgaaa cacaaagttc gagtctttcc
    14701 tagcgaatta tccggtgggg aacagcaacg tgtctctatt gctcgggcaa tagtgaatac
    14761 gcctaaagta cttatcgcgg atgaaccgac aggaaatctg gatccagaaa actcatggga
    14821 aatcatgaaa ttattagatc ggatcaatgc ccaaggaact actgtcgtga tggctacaca
    14881 taacagtacg atcgtaaata cgattcggca ccgagtaatt gctatcgaaa acggccgcat
    14941 tattcgagat caagcggaag gggaatatgg atacgatgat tagaaccttt ttccgccatt
    15001 tattagaaag tatcaagagt ttgaaacgta acggctggat gacagttgct tcagtcagtg
    15061 cggtaacgat cacattgact ttagtcggtg tattcatggc agttatcatg aacgcaacta
    15121 aattagctca agatatagaa ggaaacgttg atgtctctgt attcgtagat atcggtacaa
    15181 aagacgaaga gatgaaaaag ctagaaaaag aattgaatga cttagatcat gttaaaaaag
    15241 ttgaattttc aagcaaagaa caggaattga aaaaaatcca aaacgaaatg ggtgattcct
    15301 ggaaattgtt tgaaggtgac aataacccgt tatatgatgt gtatgtagta agtgctactg
    15361 atccttctta tacaaaacaa gtagcagaag aagcagcaga tttgaagaat gtttctcgtt
    15421 ctgattacgg tggagcttcc tcagatcgga ttttccaaat cgctggtgca gttcggacat
    15481 ggggattagt tgcagcggca ttgctgctgt tcgtagcgat gtttctcatt tcgaacacga
    15541 ttcggatcac gattttgtct agacagcgtg aaattcaaat catgcgttta gtcggtgcaa
    15601 aaaatggata tattcgctgg ccgtttttcc ttgaaggtgg ctggatcggt ctgatcggtg
    15661 cgattcttcc gattttgatc atcacatttg gttatgcatg gtttttccaa ttgatcaatc
    15721 catcattatt acgttcccat tattcattga ttcatccaca aaatatcgtt tggaaaatca
    15781 atcttttaat ggtcggcatt ggtgtgatca ttggttcact tggctcgatc atttctatga
    15841 gacgtttctt gaaattctaa taccacaata taaataaaat gagaaagttg gacagtttcg
    15901 ttccagcttt ctttttttat tggctaggag aatagaagga agcggctttt ttccctttac
    15961 tttaaaaaac aacttgggta ttcttaagtt ggtagaagaa gaggtgaagc cgtgaaaatt
    16021 atcgttgaca atttttttac gatccttttg attatgaata ttttgctttc ctttatcatc
    16081 gtttttcgag aaagaaagga aactgctcaa acatgggcat gggtgctcgt tttgatgttc
    16141 attccagttg taggatttat tttatatatt tttttaggaa gagggatttc caaagagaaa
    16201 atctttgatt tgaagatcca agggaaaatc ggtaagaacc tggaaattga agaagacaaa
    16261 caggcattga tgcgagggct ttatcctcat ccgcctactg gacaagtaga tgtgaaacaa
    16321 ttgatctata tgctcacggt atttgaaagt acgctctata cgactaaaaa tgaaatcact
    16381 ttatttacag atggtcgtga aaaattcgat gcgctgattg aagatatcaa gcaggcagaa
    16441 gaccatatcc acttccagta ttatatttat cgcagtgatg cattaggtga agaagttcga
    16501 gatgcattga cagaggcggc aagacgaggg gtaaaagtca gagtccttct tgatgcatgg
    16561 ggttccacac aagtttcttc cagctttttt cagaatttga aaaaagcagg cggagaagtc
    16621 gcatttttct ttcctctatt tgttccgtat attaatcctc ggatcaatta tcgcaatcat
    16681 cgtaaaatcg tagttattga tgggaaaatc ggatatacag gtgggttcaa tgttggaaat
    16741 gaatatctag gattggtaaa gaaatttggt tattggcgag acaatcattt gcgtatttac
    16801 ggagaagcgg tgtatacctt gcaaaaccgt tttttgatgg actggaattc tcagcataca
    16861 aaagaagtag gctattctcc caaatttttt ccgtccattc actctactgg tgatatagct
    16921 gttcaaatcg tgacaagcgg accagatacc gaacatgaac aaatcaaaat gacttatttg
    16981 aaaatgattt cgttagccaa acgggagata ttgatccaaa cgccttatta cataccggat
    17041 ggatcgattc atgaagcact gaagttggct cttctttcag gcgtaaaagt tcatatccaa
    17101 atacctaaca aaccagatca tcttctcgtt tactgggcaa cgtattcttt cgctgcagag
    17161 ctaatcgagt atggagcaag gatcgaaaca tatgagaatg gatttattca tgcaaagacg
    17221 atgattatcg atggtggcat tgtttcagtt ggctcagcaa atatcgatgt tcgttcattt
    17281 cgcttggact ttgaggtcaa tacactggtt tatgatgaac gaatggcttc acgtgtaaga
    17341 aaagcttttt ttgaagactc gaaaatttct acgcatctca caaaagaaat gtatgaaaac
    17401 cgaggaataa tcatcaagat gaaagaagga ttagcaagat tgatttcgcc gctcctttaa
    17461 aacataaaaa ggacatgaga accatcaaca gatggagatc tcgtgtcctt ttctaacggg
    17521 gccatttctg agagtacgtc agaaatggag aaagcgtctc gcatgagcga aattaacttt
    17581 ggggtatctc ttgttttttg gggaaagcaa gaaataagct aatatatgct attaaaagag
    17641 acacatttca aagccagtat agcatagaaa cttttaatga gatagttttt tttacaatta
    17701 tgagaattta ttttgttatt taatgatttt gattcgtttt catcaagtaa tgaaaatgat
    17761 aaaataaaca tactttagga ttaaaggaga tttctaatga aaaaacttaa attattctta
    17821 ccaataatag gattagctat tcttttatca ggctgttcaa agtggattga tcgaggagaa
    17881 tccattacgg ctgttggctc ttctgcttta cagcctttag tagaaacagt tgctgaaact
    17941 taccagacag aaaatcctgg aaaattcatc aatgtacaag ggggcggaag cggaactggt
    18001 ttgtctcaag tacagtcagg agcagtcgac atcggaaact ctgatttgtt tgcggaagaa
    18061 aaatcgggga tcgatgcctc agcattagtc gatcatcgag tagcagttgt tgggatcact
    18121 cctattgtga acaaggatat tggcgtaaaa gacatcagta tggaaaacct gaaaaaacta
    18181 tttacaggcg agatcaaaaa ctggagtgaa gtcggtggaa aagatcagcc agtagttata
    18241 ttgaaccgcg cttcaggaag cggaacccgt agcacgtttg aaaaatgggt attggacgga
    18301 gaaacagctg ttcaggcaca agaacaagat tcaagcggaa tggtgcgcca gatcgttgcc
    18361 gacacacccg gtgcaatcag ctatgttgct ttttcttatg tttccgatga tgtagatacg
    18421 ttgaatgtta acggtgtaga accgacagat gaaaatgtga caacgaacaa atggacgatt
    18481 tgggcatatg agcatatgta cacaagagat catccaaaaa aactgacaaa agaattttta
    18541 gactatatct tatctgatga agtacaagat aacatcgttg gagaactagg ttatatccct
    18601 gtttctcata tgaaagtcga acgtgattgg caaggaaata tcgttaaaga gtaatcttta
    18661 cacaaaattt acaaacttag ctaaactttt acatgttatt aaaaccaaat tgatgttcaa
    18721 tttgtacact gagtgagtat gttaaacctg attaaggggg agtcatctgt ggaagatgtg
    18781 aaaaaagtat tattaacaaa atcaaaacgt tcaaaaatgg aacagcgtgg aaaattcatc
    18841 agttttttat gtatcgcgct gatcgtgttg gttgtattat ctatttttta ttttgtagct
    18901 agtaaaggat tggccacctt ttttactgat aagatcaatg tatttgattt tctgtttggt
    18961 acagattgga atccaagcca aatcggagca aatgggaaac caatggtagg ggcattgcct
    19021 atgatcacag gttcgtttat tgtgaccttt ttatctgcta tcgttgccac gccatttgcc
    19081 attggtgcag cagtattcat gacggagatc tctcctaaaa acggaacgaa gatcttacag
    19141 ccggtcatcg agctgcttgt aggtattcct tctgtcgttt atggttttat cggtttatca
    19201 gttatcgtgc cttttgtcag aagtattttt ggcgggactg ggtttggtat cttagctggt
    19261 acatttgtgt tgttcgttat gatcttgccg actgtcacaa cgatgactgt tgatgcatta
    19321 aaggcagtgc cacggcacta tcgggaagct tcattagcgc ttggtgccac aagatggcaa
    19381 acaatctaca aagtcgtgct acgagcagcc gtgcccggga ttttaacagc agtcgttttt
    19441 ggtatggctc gtgcgtttgg tgaagcctta gctatccaaa tggtcatcgg taatgcagca
    19501 ttgatgccaa caggtcttac tactcctgct tctacattga cgtccatctt aacaatggga
    19561 atcggaaata caatcatggg aacagttgaa aataatgtgt tatggtcatt agcgctgatc
    19621 ttgctattga tgtcattatt ctttaatatc gtgatccgta tgattgggaa gaaaggagag
    19681 ctgaaataat gaacgcaaaa cgctcagata aaattgctac aggtgtttta tatatagttt
    19741 cggggatcat cgtattgatt ttagcagctt tactacttta tatcttagtt cggggaatac
    19801 cgcacatttc atggtccttc ttgaccgaac cttccaaagc ctaccaagaa ggcggtggaa
    19861 tcgggatcca gctatttaac tcgatttatt tgcttttgat cacgatgatc atcagttttc
    19921 ctatttcttt aggtgcgggt atctatcttt cagaatatgc tggaaaaaac tggctaacag
    19981 atgtgattcg cacgtcgatt gaaatcttga gctcgttgcc ttctgtcgtt gtcggtttat
    20041 tcggtttctt ggttttcgtt attcaattcc agtatggttt ctcgattatt tctggtgcat
    20101 tggctttgac tgtattcaac ttgcctcttt tgactagaac agttgaggag tctttacaag
    20161 cggtccatta cacacaacga gaagcaggat tggccttagg tttgtcacgc tgggaaacag
    20221 tgataaaagt ggtggttcca gaagcgacac cagggatttt gactggtgtg atcctaagtt
    20281 ctggaagaat ttttggtgaa gcagctgcat tgatctatac ggctggacaa agtgcacctg
    20341 cgttagattt tacgaactgg aatccattaa gtgtttcaag cccgatcagc attttccgtc
    20401 aagctgaaac attagctgtc catatttgga agatcaatac agaaggcacg atgcctgatg
    20461 gtgtttctgt ttcggctggc gcttctgccg tgttgattat tgctgtttta ctgttcaact
    20521 tcggcgctcg tgcgattggc aaacgattat accgaaaaat gacttcagcc taaggagaag
    20581 aagcaaaatg aaaaaatata actggaatga aacaaatgtc atcaccatgc caaaagagaa
    20641 agaagtagca ctatatacgg atgatctaca tgtctggtat ggcgacaatg aagcaatcaa
    20701 aggagtggat ctggaatttg aaaaaaataa gatcactgca ctgattggtc cttccggctg
    20761 cggaaaatct acgtatcttc gttcgttgaa tcgaatgaat gatgggatcg ctaatacgaa
    20821 aatcactggg aaaatcatgt atcaaggcgt agatattaat gcgcctcaag tggatgttta
    20881 tgagatgcgc aaacggattg gcatggtttt tcaacgtcct aatccgttca gcaagtcgat
    20941 ttatgacaat attacatttg ctttgaaaca gcacggcgaa aaaaacaaaa agaaattaga
    21001 tgaaatcgtt gaaacaagct tgaaacaagc tgccttgtgg gaccaagtaa aagacagctt
    21061 ggacaaaagt gccttggctt tatctggcgg gcaacagcaa cgtttatgta ttgctcgtgc
    21121 gatcgcgatg aaacctgata tcttgctgct agacgagccg gcaagtgctt tggaccctat
    21181 ttcgacagga acggttgaag aaacacttgt aaacttgaaa gaagactata cgatcatcat
    21241 cgtgacgcat aacatgcaac aagcagctcg tatcagcgac tatactgcct ttttctattt
    21301 aggaaaagta atggaatatg acaaaactaa caagatattt accagaccaa aaattcaggc
    21361 aaccgaagat tatgtgtccg gtcattttgg ttaagacgga ggcctcaaca tgacaaaaga
    21421 gattatttca tctaaagatc ttcatctgta ttatggaaag aaagaagcac taaaagggat
    21481 cgatctaagc ttcaatcaag gggaaatcac tgcgatgatc ggtccttctg gttgcggaaa
    21541 atccacttat ctgcgttctt tgaaccggat gaatgacctg attccgaatg taacgatcac
    21601 cggaagtgtt ttatataaag acaaagacat ctatggtcca aaaacagatg ttgttgaact
    21661 aagaaaagaa atcggcatgg tattccagca gccaaaccca tttccttttt ctatctatga
    21721 aaatgtcatc tatggattga aattaaaagg ggaaaaagat aaaaaagtcc ttgatcaagt
    21781 agttgaagaa agcctgaagg cagcgtctgt ttgggatgat gtgaaagaca aattgcataa
    21841 aagtgcttta tctttatccg gtgggcagca gcagcgtgta tgtatcgcac gtgttttggc
    21901 agttaatccg gaaatcatct tattggatga accaacaagt gcgcttgatc ctgtttctac
    21961 tgggaaaatc gaaagtatgc tgttagaatt aagagagcgg tatacgatga tcatcgtgac
    22021 ccataatatg tctcaagctt cacgtatttc agataagacg gcattctttt tagatggtca
    22081 cttgatcgaa tacaatgata cgaagaagat attcatgaat ccagaaaaac aagagacaga
    22141 agattatatt tcaggtcgat ttggataagg aggaaacgga atgctacgta cacaatttga
    22201 agaagaattg ctgaatctcc ataatcagtt ctacgaaatg gggatgatgg tgagcagcgc
    22261 cgtgcacaag tcagttcgtg catacatcaa acacgataaa atcttagccc aagaagttat
    22321 cgaaaacgat ataaatatca ataacatgga aacacgcttg gagaagaaaa gttttgaaat
    22381 gatcgcattg caacagccag ttacaactga tttgcgtatg atcattacgg tcatgaaagc
    22441 tagctcggat ttagaacgta tggccgatca tgcggtgtct gttgctaaat ccacgattcg
    22501 cgtaaaaggg cagacgagaa taccggaaat cgaaaaagag atctccgata tgtccgatta
    22561 tgtgaaaaaa atggtggaca acgtcctagt tgcttatgtc aaaacagacg aaaaagacgc
    22621 acgtacgatc gccaatatgg atcaaagagt caatgactat ttcaatcgaa tctataatgc
    22681 aacaatcaaa aatatgcagg aaaatcctga gacagtcatc agtggaacag actacttgaa
    22741 tgttgccagt tatctagaac ggatcggaga ctatgtgaca aacatctgtg aatggatcgt
    22801 ctacttggct acagggaaaa tcactgagtt aaatacgaac cacaatgaga ttttctaaac
    22861 tgaagctgtg attatgccgt ttgtttagga aaagaggttg tgaaatcagc ggttgtgaaa
    22921 aagctgttct gcatcaagta ataagaacag tcaaacaaaa aatagctcct catatttttc
    22981 gtttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt cgggaaaggg
    23041 tttgtgacaa aacatgtcac aaaccctttt catcatttca tagaaaggat aaagaatgca
    23101 aaaaaaaatt agcatcatca ctatattttt tatttgtgtg cttacttcat gggttattca
    23161 gcatcaagcc gctttatatg aagtacctgt catcgagata gaaaaagtac gaataaaaga
    23221 ggatcatcaa ttgattacag gaaaactgat gaacacggat caaaaaggag aatcgattta
    23281 tttatcggtt ccttatcaaa aaaatcaaat agaaacagca aatataaaag cgagacaaat
    23341 gtatctagtc caaatgcatg gagatgaatg gcagctagta gcaaaaaaaa atgatggctg
    23401 gcttttcttc ttcacaagta tcttcattgg cgccatgttg cttattggtg gaaaacaggg
    23461 tctatttgct ttaggaagtc tcatcatcaa tatcgtttta cttttacttt tgttgctcat
    23521 ctttactaaa acagaaggga tttctctatt aaatatatct atcctgttcg tcgttatagg
    23581 gatgattatt agcatattgg ctttagatgg atggaataga agcagtataa tcaaaatttc
    23641 agctgcagtt acgagtgttt ttttagcatt ttttatttgt cttttgacaa tgaatcattg
    23701 gaaagaccaa ggactgcgct atgaggaact gaattatctg acacgaccgt atcgttctgt
    23761 ttttctttct agcgtactga ttggaacagc tggaggaaca ctcgataccg ttatcacagt
    23821 cattgcgaca ttagaagaat tgactaagcg acaaccagaa ataacagcga aacagttatg
    23881 gctttcagga aggcaagtgg gtcgagatgt gattggctcg atgaccaata tccttttgtt
    23941 cgtctatatc agtgggtcag ttccatcgct tcttttatat ttaggaaatc aatggtcgtt
    24001 caaagaaacg atagaacagc atttatcatt ggaattttta cgcgtcattg ctggcagctt
    24061 aggaatagta ttatcgatcc ctatctcggt cctctttttt ctgactgtaa ggaggctgaa
    24121 acgatgaatg tacttattat acttgttggg attttagcaa tcagcgtgct acttattggc
    24181 ggtacgcaag gtatgtatat tcttttaggc ttattcatca atttagggat ctttttcctg
    24241 ttgttattcg gttatcatca gaagtggccg atactggcat tgtcggtcat aggattttta
    24301 ttgattgctg tggttatctt attttttatc aacggttata atctaaaaat gcgtgcagct
    24361 tttgtgagta tactgatctt tttattttgt ttcctactat tgcttcctat cactgatttt
    24421 ttagcaatcc aaggttttac atcggtagaa ctagaagaat tatctggctt agataagact
    24481 ttagcgattg attttagatt acttacgaga tcgttgcttc tgattagtct tagtggtgct
    24541 gttttagatg cgagtgtagc aatttctagt gggacattcg aagtatatca ggcaaatccg
    24601 caattatctt tttaccaatt gcgccattct agtttcgcta ttgctaaaaa aatattggcg
    24661 tcaacagtaa tgacacttct ttttgcattc atgggaagtt ctttagcgtt gatcctctgg
    24721 tttattgatt tagacattcc ttttcagcaa gtgatcaatg aaaaatcatt tgttctagaa
    24781 tatacgatgg ctattcttac cacattgtcc gcactacttg ttcttccttt aacctgcgtg
    24841 actacagcat atctctttac aaaaaagaaa gaacaactca agatggagta aaagttacaa
    24901 cttaagtcct atgacatttt tactgaatca gtgcataata tggatgtaag gaaaagcttg
    24961 caaacaattt tgcttaagga ggcaatttcc atgaaagaaa gagaacgtat tttagattta
    25021 gtgaaaaaag gaatcctttc gactgaagaa ggcttagatt tattagaaag catggcaact
    25081 gaaaaagacg aaaagcaaat caaaaaagaa gcagacaagg taactgctag tcataaagaa
    25141 aaagatcagg caagtcaatt gatcgataaa cttgaaaacg gcgaagaaga aatttctgag
    25201 ccagttgatc caaaggaaag agaaagacaa gatcaagaaa acttagaaaa gatcttagat
    25261 gaactagcaa ctgaagcgaa taaaacttcg gcgcgtctag atgaagtaaa tgctcaattc
    25321 gctgacaata aggcagcacg caatgagaaa caggaagcat tgatgcagct gaatacaaaa
    25381 gaagaattag gcgagttgac agaagaagaa ttagcacagc gtcagacgtt agaagcagaa
    25441 atcaaagaat tagaagctag tggcgataca ctgttagaag aacaattgaa attagaagca
    25501 gaattaaaag atatccgtaa gaatcaatgg tctgagaaaa aagaaacgtt tactgataag
    25561 tttgaattgc cagatgattg gaaagatcaa gcaacagata cactgaatca agtaggcgaa
    25621 aaaatgtcag aagctggatc acagttaggc aaattcttga agaaaacatt ccaaactgtt
    25681 tcagaaacag tgaatgacaa catggaatgg aaagatgtca gcttacgtgt ccctgggatt
    25741 gcgacaacga aatttgagca tgaattttac tatgaagctc ctgcagcaag tatccttgat
    25801 atcaaggcag caaacggaaa cgtgacgtta aaaacgtggg acagcaacga tgtgaaagtt
    25861 gaagctaaga tcaaacttta cggaaaaatg ggtgctgaac catttgaagc attctctgaa
    25921 cggagccaga ttgaggttaa tgaagatcat atctccttcc aaattccaaa taaacgtgtg
    25981 cgtgcagacc ttgttttcta cttacctaaa cgagtatacg accacgcagc tattaaactt
    26041 ctaaatggca acatcatgat tgaaacattg gaagctaaag atatttatac aaaatctacg
    26101 aatggtaaca tcatcgtgga ccaattaact gcaacgatgc tggaagtaga aggtgtaaac
    26161 ggcaatatcg atattcgaaa cgggaacatc ttagattcta ttatcgaaac agtaaatggt
    26221 acagtgacat ttggtgcgac agcagaaaac ctttctgtgt ctttggtaaa tggtgatgta
    26281 cgtcttacga ttaaagaaga taatttgaaa aaagtagaag caagttcagt gaacggaaat
    26341 gtcaaagtcg ctttaccaga tggcatcggt ttggaaggac atgctaaaac aagtcttgga
    26401 agtatcaaca gccgtctgtc caattacgaa gtagttcgtg agaaaaaaga acggacaaac
    26461 caaatgttgc aattccgtcg tgtttcagat ggtgaaatcg cgcaagtgca actttcaacg
    26521 acaacaggaa gtatctattt aaaagataca gataaataaa gaggttgtga aagagatgtt
    26581 cagccttgag caataagaag cgattttgta aaatagcttc ccctatttta caaaattgtg
    26641 ccttattgcc gatagtctat ctcttgaaca ccgtttacca agagattatg aacctgccaa
    26701 ttatcgccga gtcacaagga ataatttcag aaaaagctcg tcacgtttta tgaatatggt
    26761 aatttgtgac cgagaatttt gcagaaagac accgattaag aagaaggttg tcacaacgat
    26821 tttcttcatt taaaatagta gaacagaggt gagacatgat gaaaaaaaag ttgacaaaat
    26881 ctaataaaaa tgttgttttg acagggactt tagcaggaat aggagaatac atcgggattg
    26941 atccgactgt tatccgtgtg ctttatgtat tcctgagctt agttgctttt ggaagtccaa
    27001 ttattttata cattttatta gctttgatta tcccatctgc gcgttctgca cgaacaaact
    27061 atggacataa caatgcatat tatgaaaaaa acaattatcg tgaacaaact cgtgaacaaa
    27121 caaaaaaacg caaagaagcg gaaaaaatcg atgatgacga atggagtgac ttttaatgtc
    27181 gtactttcaa cgtattttag ttaatacgtt aacattcata tcattggctg ttctttttcc
    27241 tgcgatggtc catgtgagca gtatttggac ggccgttctt gccagcttcg tattgtctgt
    27301 gttgaatatg tttgtcaaac cgattttgac gatactgtcc ctgcccttta cattagtcac
    27361 attgggattg tttacgtttg ttatcaatgc agcgattctt aaaatgacct ccttttttgt
    27421 gggagcagaa cattttagtt tttcttcatt cggtgcagcg ttgttagtag ctatcttgat
    27481 gtcgcttgtc aatgtaattg tcagcgaaca taacagtaga aaataaaaga aagctgtgcc
    27541 gaactatcgg cgcagttttt ttatttagaa tcaggaatgc gttttctatt acattggtaa
    27601 ttaaataaaa acgttttttt taattggtag tacttttggc tagacaatga taaaatgata
    27661 gagaacactc taatagaaat gaggtagcat gaatgtcaga agttgtaaaa gttagccaat
    27721 tagtgaaaaa attatctttg gaaatcgtga caggcgacga gaaaagctta gaccgtgtca
    27781 tcgtaactgg ggacatctca cgcccaggac tagagttgac tggttatttt aattattatt
    27841 cccataatcg tttacagcta tttggcagta aagaaatcag ttttgcggaa cggatgatcc
    27901 cagaagagcg tttgatcatc atgagaagat tatgtagtga agatatgccc gcatttatta
    27961 tttcaagagg attaccagct ccagaagaaa tgatccaggc agcaaatgaa aatgggatag
    28021 ctgtactgcg ttctcccatc tcaacttctc gtttattagg agaattatcc agttatttag
    28081 atggtcgcct tgcccctcga acaagtgtcc acggtgtttt agtggatgtt tacggattag
    28141 gtgtactgat tcaaggtgac agtgggatag gaaaaagtga aacagcgttg gagttgatca
    28201 aacgcggcca tcgattgatc gcagacgatc gagtagatgt ttatcagcaa gatgaattaa
    28261 cggtgatcgg agaaccgcct aaaatcctgg aacatctgat tgaaatccga ggaatcggga
    28321 tcattgatgt aatgaatctt tttggtgcga gtgcggtacg tggctctatg caagtccagt
    28381 tggctgttta tctggaagca tgggcaaaag ataagaaata cgaccgtctg ggctctgaag
    28441 acacgaccgt tgaaatcgcg gaagtgggca ttccacaaat aaaaatccct gtcaaaacag
    28501 gacgaaacgt tgcaatcatc attgaagtag ccgcgatgaa tttccgtgca cgtacaatgg
    28561 gctttgacgc aacgaaaaca tttgaagaaa gattaagtcg cttgattgaa gaaaactcag
    28621 gcaacgacta gcaaggagtg acaatatgtt agcacaaatc aatcgagttg cttttgagct
    28681 tgtagggatt cctatctact ggtatgcatt gatcatcgta tcaggtatca ttatcgctat
    28741 gtggcttagc agcagagagg ctgttcgggt aggattaaaa gcagatgatg tgacagattt
    28801 tatgttgtgg ggattgccta tctccattat tggcgctcgc ttatattatg ttttatttga
    28861 tcttccccag tatatagctg atccaatcca aatattcaat atccgttcag gaggcttagc
    28921 tatttatggc ggactgattg caggcgcgat cacgctgtat ttttttacta aatatcattt
    28981 tatttctttg tggacttttt tagatatcgc tgcaccaagt gtattattag cacaggcaat
    29041 cggtcgttgg ggaaatttca tgaaccacga agcatatgga ccagacacga cgcgaagctt
    29101 cttagaaagt ctgcatttac cgcaattcat cattgataat atgtatatcg aagggatcta
    29161 tcgtcaacca acatttcttt atgaatcact ttggagtttg attggattta ttttgctttt
    29221 gtttttacga aagaaaccac accttttgaa aaaaggcgaa gtccttttat gttatctaat
    29281 gtggtatagt ttcgggcgtt tctttattga aggattacgt acagacagtc tttacttttt
    29341 aggtgtgatc cgtatttctc aattattatc tgctgtgtta tttgtcggta cattgattct
    29401 atttatttgg cgcagaaaaa aaggaaattt accggattat gaccgctcat taggaaaaaa
    29461 tcaacaaatt atctgatttt tagagcgtta aaaatgaaaa aaatttcagg agtggtaaaa
    29521 tgaattatag agtagcggtt ttaggaccag gctcttgggg tactgctttg gcacaagtcc
    29581 tcgccgaaaa cggacatgac gttcggattt ggggacatcg tgcttcacaa gtagaagaaa
    29641 tcaacaccca acacacgaat caacgttatt taccagatca ttatctaccc gattccatcc
    29701 aagcattcac caatatggaa gatgcagtaa aagacgcaga tgcggtgcta ttcgtcattc
    29761 caacgaaagc aatacgttct gttgcaaaag aactagtgcc taaattgaat acaaagccag
    29821 tgatcatcca tgcaagtaaa ggattagaac agggaacaca taaaaggatc tctgacatct
    29881 tgttagagga aatcccagca gaaaaaagaa aagctgttgt tgttctttct ggaccaagcc
    29941 atgcagaaga agtagcaaaa catgacatca ctacgatcac agctgcaagt acagatgcaa
    30001 aagcagcaaa atatgtacaa gagctgttca tgaataatta ttttcggatc tataccaatc
    30061 ctgatgtgat cggtgtagag atgggggcag ctttgaaaaa tattattgct attggtgctg
    30121 gcgctattca cggcttaggg tttggcgata atgcaaaggc agcaatcatg acgcgcggat
    30181 tagctgagat cagccgttta ggtgtagcaa tgggtgccaa cccgctgacc tttatcggat
    30241 taagcggaat aggcgatcta gtcgttactt gcacaagtgt tcattctagg aactggcgcg
    30301 caggcaactt gttaggacaa gggcagcccc tagaagaggt gctagccaat atgggaatgg
    30361 tcgttgaagg tgtgaatact acccaagcag cgatggaact agcccaaatg atggaaatcg
    30421 aaatgcccat cactcaaaca atctatgaag tgctgtatga acaaaaagaa atcaaaacag
    30481 ccgcaaaaga aatcatgtta cgtgatggaa aaaaagaaaa tgaattctca ttaacaattt
    30541 tttgaaggag cgaatgtata tgaaagttaa aaaagcagtt attccagcag caggattagg
    30601 aacaagattt ttaccagcaa caaaagctat ggcaaaggaa atgttgccaa ttgttgataa
    30661 accaacaatc cagttcatcg ttgaagaagc gttgaaatca ggtatcgaag acatcttgat
    30721 cgttacagga aaagctaaac gaccaatcga agatcacttt gattcaaata ttgaattaga
    30781 aacaaaccta agtgaaaaag gaaagaccga attattgaaa ttagtagaag aaacgacaga
    30841 tgtcaacttg catttcatcc gtcaatctca tccaaagggt ttaggacatg ccgttcttca
    30901 agcaagagcc tttattggaa atgaaccatt tgtcgttatg ctaggcgatg atataatgga
    30961 agacgaagta ccattgacac agcaattaat cgatgattac gaacgtactc atgcttctac
    31021 gatcgccgtt atgcaagttc ctcatgatga aacttcaaaa tacggaatta tcaatccagg
    31081 agaagtattg gaagatgggt tatacaatgt caaaaacttt gttgaaaaac cagatccaag
    31141 caaagcacca agtgatttag ctattattgg acgttactta ttgactccag aaatttttga
    31201 tgtattgtcc gagcaacaac caggagccgg aaatgaaatc caactaactg acgcaatcga
    31261 tacattgaat aaaacacaac gcgtatttgc ccgtaaattc acaggtaaac gttacgatgt
    31321 cggtgacaaa tttggtttta tgaaaacaag tattgaatat ggtctaacac atccagaagt
    31381 aggtgcacct ttacgtgagt acatcctgtc tttaggtaaa acattagaag cagaaggaag
    31441 caagatcgat cataatccaa aaggaataac agaaatcaaa gaagaaaaga aaaaataacc
    31501 cttttttcta acgagcttca gctgctaatt gaataaaaag tagttgaagc tcgtttttat
    31561 ttgtttaaaa aacacgaaaa tgctatcctt atagaaaaga gagacaagaa gggagtttga
    31621 tcatgtcagg aggagaaatt gcaggattga tcgcagcaat cgcctttgcg atacttgtcg
    31681 tttttatcgt tcgtgtgttg ttgcaagttt ctaaaacttt agaaaaagta gatcagactg
    31741 tagcagaagc aaatacgacg atcgaaatcg tcacaaaaga cgtagatctt ttgtctagac
    31801 aagtagaagg cttactggtc aaaagtaatg aactgctctt ggatatcaat ggaaaagtcg
    31861 caacgatcga tccattgttc acagcagttg ctgatttaag tgaaagtgta tcagaactta
    31921 atcattctag taaaaacatc gcgacgaaag tcggcggaat cggtaaaacg acagctaaag
    31981 cgacggttgc cggaaaagtc ggtgggacag cgatgaaatt ttttaaaaac aaaaaacata
    32041 ctgaaacgac aggaggaaaa taaaaatggc taaaaaaggt ggatttttat taggtgcagt
    32101 agtcggaggc acagccgcag caatcgccgc gttattgtta gcaccaaaat ctggtaaaga
    32161 acttcgggat gaattagctg cccaagcgga tgatttgatg gatatggctt cagattacac
    32221 agatatggcg aaagaaaaat cgactgagtt aacaggtatt gcaaaagaaa aagcgcaaga
    32281 tctttctcaa caagcaggtg atctagcagg tagtgtcaaa caaaaagtaa gtgaatcagc
    32341 agatgaagca ggaagtgaat ctgacgatat cttgttaaat cttaaagaac aatcagctga
    32401 cttatctaat cgtttcaaaa aaacggcaga agatatgaaa aaacctgcag atgaattagg
    32461 aagtattgct aaagatactt cagaagatat catgattgat gtaaaagacg ctgctggtaa
    32521 agcaggagat actgtcaatg aaggaatgtc agaagcaaaa ccagttgcag atgactcaaa
    32581 agaaatgatg aaaatggcaa aagaagatgc gaaagaagct aaaaaagaaa taaaagatac
    32641 aatgacagat tcatctacaa atcactaaaa tcacaaattt atagaataaa aaggtgggag
    32701 cggactagct ctcacctttt ttactcttat ttttctaaaa caagtaattc ttttggtgta
    32761 gtcgtgaatg gcacacagcc atcgcttgtc acatatacac agtcttcgat acgtacccct
    32821 actttttcag gaatatagat tcctggttca agagagaagc acatcccttc ttcgattact
    32881 aagtcatttc cttctacaag ggaagggaat tcgtggacgg ttgttccgat cccgtgacct
    32941 aaacggtgat tgaaatattc gccgtagcca tagctgttga tcacgtttcg tgcgatttga
    33001 tcaagttcac ctgctgtcac acctggacgg acggcttctt gtgcggcaag ctgtgcttca
    33061 agagtgatgt tgtagatttt ttcttggaag tcggttggtt tttgataagc aattgtacga
    33121 gtagcatcac tgcaatagcc attccaaaca acgccaagat cgaataacac aagttcattc
    33181 ggtgaaatct tcgtatttcc tggtgtacca tgagggcttg cagcatttgc tcctgcaagg
    33241 actaatgtgt cgaaagacat ttgtgaaaca ccttgttttt tcaattggta ttcaatttct
    33301 gcaatgatcg cttgttcagt cacgccttct ttcactgctt taaagccaat ctcaaatgca
    33361 acatcggccc atttaccagc ttcgatcagt ttgtcttttt cctctagagt tttgatcagt
    33421 tgtagacgtt ggatgatgga agttagatca gtgtaagaat ccgttcccgt caagatatca
    33481 gagatagcat cgaagcgctc gacagtcaag gcgttctttt caatccctac attgccaggc
    33541 tgaccatagc gagcacgaag ttcttttccg atgatctccc aagggttttc gctgtctaaa
    33601 tagccacgga catcatacgc ccaactactg ttttgagcat cttcaacttc caaagcagga
    33661 gtgaaaagga aaggatcttg ttcaagaggg ataaaaagag ctagtacacg ctcatgagga
    33721 tcactttcgt atccggaaaa gtaagcgatg tggccagggt cgctgatata agccaattcg
    33781 acttggtgtt gtgccatcca ttgacgtagt tcattgattt ttacgttatt catttggcaa
    33841 cctactttct aaattaattt tgtattcaat aattgtacca ctgatttatt aaaagaccaa
    33901 cgttctaaaa actgaaaaaa gtaaaaattt tcatgaaaac agagataaaa cggttgcaat
    33961 tcttgagatt atttgatatt ctattttaga aatgtaagac gtattttcag aaaacatata
    34021 aaatatgaat aggagaacac aaatggaaaa gcaaacaatc actatctatg atgtagcgag
    34081 agaggcaaat gtttcgatgg ctactgtttc acgtgtcgta aatggcaatc caaacgttaa
    34141 accagcaaca agaaaaaaag tcttggaagt tatcgatcgc ttggattatc gtccaaacgc
    34201 agttgcgcgt ggcttggcaa gtaagaaaac aacaacagtt ggtgtaatca tccctgatgt
    34261 cagcaatatg ttctttgctt ctttagcacg tggtatcgat gatgtggcaa caatgtacaa
    34321 atacaatatt atcttagcaa attcagatgg caacgaccaa aaagaagtca atgtattgaa
    34381 taacttactg gctaagcaag tggacggcgt gatcttcatg ggtcaccata tcacagacga
    34441 aatccgtgga gaattttcac gttcaaaaac tcctgttgta ttagctggct ctattgatcc
    34501 agatgaacaa gtaggtagcg taaacattga ttatgtatcc gcaacaaaag atgcagtaaa
    34561 caaattggca aaaagcggca ataaaaaaat cgcttttgtc agtggtgcgt tgatcgatcc
    34621 catcaatgga caaaatcgtc tgaaaggcta taaagaagca ttaagcgaaa ataacctacc
    34681 atatagcgaa ggattgatct ttgaagcaca atacaacttc aaagatggtc tatctctagt
    34741 cgatcggatc cataatagcg gtgcaacagc tgtctatgtt tcagatgatg aattagccat
    34801 cggtattttg gatggtttat tagaccgtgg cgtgaaagta ccggaagaat tcgaaatcat
    34861 cacaagtaac aattcactac tgacagaagt agctcgtcct cgtttaacaa gtatcacaca
    34921 accattatac gatttagggg cggtatctat gcgcctattg acgaaaatga tgaataaaga
    34981 agaagtcgaa gaaaaaacga ttatcttgcc ttatggaatc gaagaaaaag gatctacaaa
    35041 ataataaaag gacaaccatt cagaggttgt gtgcctgccg tttaactctg cgttacaagg
    35101 agcaatttca aaaaacagca tctcatgttt gatgaatatt gtgactcgtg attcggagtt
    35161 ggcaggagaa caccgtttat ttgggaaaag gggttatgac aaaattttgt tataatcctt
    35221 tttttgtgtc ttaaaaaaga gagaaagtgg ttgaaattac atttatcgta tataaaacaa
    35281 tccgtgtata gatgctgcat tttgttcacc accatcaaaa aaataaggga ctgtgacaat
    35341 actattgtca caatccctta ttactgtgta ttctattaaa tcaatcatct ttctcatctg
    35401 attctttagt tgcttgattc gctcttgcat aagctgaagc ggtgttccag taatctgtat
    35461 agttttcatc ggtaccgcca acgccaaatt taaaggcgct tttcttcggt ccatttttcg
    35521 cccatagcga ttcaactgtt tttcctggtg tttggatgga ttttttattg acagtaactt
    35581 ttccaggtgt ttgaccagta aatccagata cttttttctt catcacatca gatgataatt
    35641 cgaatttctc atcagtgcca aaaatgtcag gttttgcttg gtaagcttga ttgatcagat
    35701 tggcaagata attagccgaa cgtatccttg ctttgctgtc cattccagta ttgtcatcgt
    35761 gccctgccca gctactgatg gtaatagacg gagtagagac aattagccat gaatctcggt
    35821 attcattggt agaaccagtt tttccgaccc aatctgcttt tcctagattt ccgtttaagc
    35881 ttgagatggc atctttgaat ggtgtagtga tttttgcatt gatcacacta cgcatcatgt
    35941 cattcatgat cgatgccgtt gcttccgaat agatccggac agagttgctt tcgtgtttat
    36001 aaattacatt tcctgcgtta tctgtgatgg aatcgatgat atagccttct tcatagacgc
    36061 cgccgtttgc taaagcctga aatccgttgg tctgttgaag cgtcgtgaca tctactgtac
    36121 ctaaaggagc agattcaacg ccccaattgt catttgccgg gtagttcatc tttgccaagt
    36181 agtgctcgta agcatattgt ttcgatccac cggaattcaa cacatcttga tataagtgat
    36241 aagccgggat attgttggac cattctagtg actcacggac ggtttggaag gtgttcgatc
    36301 cttcgttagt agcattgacg attttctcgc cagcattttc tccttcttgc caagttgttg
    36361 cataatctga tacacgggat tctgatccga taagccccat gtcgattgca ggtccataaa
    36421 caagaactgg tttgattgat gagccagctt gacgttctgt atcgaaggca tggttatttt
    36481 gattctgact gtaattccgt ccgccaacga atccgtagat gcggccagtt ctgttatcca
    36541 tcaatacatt tccggtttct acttgactgc cagcaccatc atctaacatg tatccataat
    36601 tttcaacgcc attttgcaat gcagcataga catctttatc gattgtcgaa tggattgtat
    36661 atcctttatt ttggatcgtt tgctgtgcct tttgataata agcgtcactg accgatgaat
    36721 cagacatatc agcgttgtct ttttcagcca attgttgtgc aatgattctt gttgcttcat
    36781 tcatcacagt ataatacaag aattctctgt cagtctgttc tgcgatttgt tgtggtagaa
    36841 agtcttttgt aaggtcatag gcttttgctt cttcgtattg ttctttcgta atttggcctt
    36901 cacgatacat actgaataaa acaaaatctt ttctggcaag gccggcagaa agatcttctt
    36961 ttagcgcgcc tgtgttcgtg tatggactat acgtgatcgg gctttggggt aatccagcga
    37021 tataagcagc ttgcggcagc gtcacatctt ttgcatctac accaaagatt ctttttgcag
    37081 cttcctggac tccagcaatg ttttgtccct tattattacg accaaatggt gagacattca
    37141 aatacgtagc aatgatctca tcttttgaaa agtatttttc tacttgtgca gaaagaatga
    37201 tctcgtttgc tttacgtttg aatgttgttt catctgtaag gatctgctgt ttgactaatt
    37261 gctgtgtcaa agttgatcca ccagaagagc caataccagt agcttcagat actaatgctc
    37321 gtaaaacagc tttaggaacg taaccttggt gtttatcgaa gtattcatcc tctgtgctga
    37381 taattgcagt tttcaataaa ggtgaaatct tatcagatga aatagtcgtg cgcatcagat
    37441 ctgtctggat ttttgatatc tctgtattat ctgcataaac aatctttgac gtttctgtaa
    37501 tgtttcctag ttctgtctgc agtgcttttt tagtcggcaa ttgtgtgtct tcgacgagat
    37561 aggcaaaata gccagcgcct attccaatac ctagagctgc gaacatcaaa agaagaacag
    37621 taataaaaac agtcaatgat tgaaaaactc taaaaataat cttaggaaca aaccattttt
    37681 ctttttttct tctattctgt ttttttcgag tatttttgcg tggcaaaagg gtcacctcaa
    37741 ctaatggttt tatttcgcta attatagcaa ataatagaac aaaaaaacag acaaggctgt
    37801 ttttggcagg aatttctttt ttaaaagagt taaaacgtga gaaaatgatc tttattataa
    37861 aagagttctt aagcttttct ttagagaaaa tttcagttac tatggagaac agggcaaaga
    37921 aaaatcaatc attttctttg tatatcatct taccaatata taggaattgt tagtaacaat
    37981 tgcctttcaa ggaggaatcc atccatgtta ttaggctcac acgtcagcat gagcggaaag
    38041 aaaatgctgt taggagcagc agaagaagca gccagttatg gatcttcgac ttttatgatc
    38101 tatacaggcg cccctcaaaa cactagaaga aaagccatcg aagagatgaa tattgatgcc
    38161 ggaaaagcgt acatggcaga aaaaggtcta acaaatatcg tcgttcatgc cccttatatc
    38221 attaatttgg ggaatacgat caaaccagaa atgtttcctt ttgccgtcca atttctgaga
    38281 gaagagattg ttcgtgcgga agctttaggc gctactcaaa ttacacttca cccaggtgct
    38341 catgtagggg caggaacaga agccggaatt gctagaatta ttgaaggctt gaatgaagtt
    38401 ttgacaaaag aacagacggc acaaatcgct ttggagacaa tggcaggtaa aggtacggaa
    38461 atcggtcgaa catttgagga attagcgcaa atcatcgatg gagtaaaatt gaatgaaaaa
    38521 ttatccgtta cttttgacac ctgtcatacg aatgatgccg gttataatat taaagaagac
    38581 ttttcaggtg taatggaaga gtttgataag atcatcgggt tagatcggct taaagtactc
    38641 catatcaatg attctaaaaa tccgcaaggt tcgcataaag accggcacgc caatattggt
    38701 tttggaacaa tcggattcga cacgctgaat aagatcgtcc atgatccaat gtttgacaat
    38761 atttcgaaaa tcttggaaac tccctatgta ggcaaagata aaaagaaagc ttacgcacca
    38821 tatggaaaag aaatcgccat gttcaaagcg caaacattcg atccagaagt gtttcctgag
    38881 ttattagcag aagaagtgat actctaagat tttctatctg acagcagcaa aaaaggacca
    38941 ggcacattgc cttgtccttt tttgtttaag aaacaaagga gtactttttg cagtcttttt
    39001 catcatgcat cttctccccc ctctttttaa atttagacaa acaaggtata ctggtagaaa
    39061 agtaaaaaaa cgaaaagttt cacaagcaag tggcaaaacg tgattggaag gacgaaaaga
    39121 tgctgaacac tttaaaaccc ttgatcaacc aactaaaaaa gaaacataca tactttattc
    39181 ttggcatagc cctattattt acaattattc gtttgtattt atatagtaaa gcaatctatg
    39241 gaacagacat gtcggcaacc tatgatgacc agctgctgat tcgctatgct caaaatattt
    39301 tggacggtaa atggctaggg gagtatacga ctacaacact ttccaaagga atctcctaca
    39361 gcttattttt agtacttgca cataaactgc atctttctta tgcagttttg ttcggcatgt
    39421 tcagtatttt tgccagtata ttggttgcac tcgcgtttaa accgattgtg aagaacaagt
    39481 atcttctttt aagtatctat ttttattttt tgttttctcc gattagtttt acacatgaat
    39541 attccactcg aacatatcgt aatgccctcg tgattccggc tgttgagatc attatcgctt
    39601 gtttattagc tatttattac cgcaaatacg gttctagcag aaaattgctg ccatggtttt
    39661 tagggctttc tgtcgctttt cctttctttt ggttcattcg cgaagattct atctggcttc
    39721 ttccttttac tgtagtagct ttgggtatca gtttcgtcca gatcttgcta gggcaagact
    39781 tttctttcaa agaagtaaag aagttaaaaa aacgactctt tcttctctcg aaacgggaat
    39841 ggctgaaact ttcattgttt ttacttccta tagttggctt ttccatcgtc tatcaatgga
    39901 tcgaatataa aaatgagaca acatacggta ttgcaaccat caatgaccgt tccagtggag
    39961 aatttggacg tttgatgaaa caactgatcc gaatagatga tgaaacgaac ttgaataaag
    40021 aaaacagtga gatatgggtc tcacatgctg cactaaataa agcattagcc gcatctccta
    40081 cattccatac tgtttctacc cgtattgacg aattatatac gactcacgcg tggactcaag
    40141 gtggaaaagt aaaagaatta cccggagata tcatcttttg ggcattgcga gatgtggtaa
    40201 gcgaatctgg ttattaccaa aacaatgcaa aagaaaccaa tgatttttgg aaaaaagtca
    40261 accaagaatt agaagaagcc tatcacaagg gaacaatcaa aaagaaaaaa gagttctatc
    40321 tgatgggatc aggagatgga aagacgatga gtgatctgcc aatcgtattc gatttccttc
    40381 aatcaggata cgcttatgca tggcgatata aagatttcca tcaaggagga aattatagtt
    40441 tcggaacgca agaacaagtc aaacaggctc aagaattatt aaaccttccg ctaatggaga
    40501 attggatcga tagtaatgaa ccgagaacac ctaatttcca attaacaaaa tcagcaaaag
    40561 tcgcgaatgt catcataaaa atttaccaag ttctggctat tcccgtgctg attgcttcaa
    40621 tagcagggat attacttatg atactaggaa ccgtatttgc aaaagctgat cgtgcctatt
    40681 atagttcctc tttgattgtg gtcaccggat taatactgac acaattggtt tttcttttcg
    40741 gtgtctcatg gttttgttct tatgcgccag aaaaaaagga ctatttttta agtgtctaca
    40801 ctggtgcagg cgtgccatta acgcaattca tcgtagtaac aagcatgcta gttttcagta
    40861 aattaaatct gaaaaaagct ttatcttcta aataaaaaga cccttggtag ctagattttt
    40921 aacctaacta tcaagggtct ttgtgtggaa aaatcagttt ttattcgatt tcttctaccg
    40981 tttcctgcaa aagctgttca tattttttgt ttcctttata taaatcacca atcgtccatg
    41041 ccaacaggag aaggacaaga acaatgatta cataagcgat gtgatcagcc aatgctagtt
    41101 gagcggaagt ggcttgctcg ccgaagaaac tttcgatctg tcctggtaat cctcgtaagt
    41161 tcaagtaagt tagactaatc acagagaacc agccaagaag ttttacgatg aatgaatttt
    41221 taaatcggct acccatctcc gcttggctgt tggtcatcat cagtaaagga atcatcgaaa
    41281 acggcaacgc aaaagccaag aacacctggg attcattcat taagttattc aaagcggcat
    41341 gttcttcgat cgttcctttt gtacttgtaa acaaaacaca aatcaataca ggaatcacag
    41401 aaagcaatcg tgtgaccagt cttcttagcc aaatcgggat acgcatatgg atgaatcctt
    41461 ccatgatgac ttgaccggtc aacgtaccag taatagtaga gttctggcca gaagcaagaa
    41521 gtgctacagc aaataaagtg gacaagatac cagattttgc tacagcaatc aaaataccat
    41581 tgctcatagc tgatggatca gataaagctt catacagacc aaaaaaggaa ggatcttgga
    41641 cagaaccggt tttgaagaca gcgacaccca tgatcaatag cagtgcattg actagaaaag
    41701 ccattgacaa ctggatatta gaatcccaag tagaaaaacg tacagcttgt gctacatctt
    41761 cttcgttttg gtgatcgatc ttccgagttt gtgaaactgc tgaatgaaga tataagttat
    41821 gcggcatgac tgttgcacca ataatcccta aagctccagt cagcggagtt tcgccgccga
    41881 tgaccggtga gtctgagaat gtatgactgt tagggataaa gccagcaaat aaactgtgcc
    41941 aatctgggtt agacaagcca acttgataga caaaaacgaa taaaatgacg aaaattaagc
    42001 agacaaccag tgcttcgatc ttacgaaacc cgacttttgt cagtagtaag agaacgaaaa
    42061 catctaatac agtaataaag acggcaatca ccaatggaat atcaaaaagt aagtagaggg
    42121 cgattgctgc accaatgact tctgcaatat ctgttgccat aatggctaat tcagtcaaga
    42181 tccataaaat gacacctaat gtcttacttg tgcgtgctcg gattgcctgt gccaaatcca
    42241 tttggctcac gattcctaat tttgctgcca tatactgtaa aagcatagca atcagactag
    42301 acatcaaaat aactgacatc aataaatatt ggaaattttg tcctccggtg atcgaagtag
    42361 accagttacc cgggtccata tacccaactg ctactaacgc accaggtcca gagtaagcaa
    42421 gaagtgtttt aaaaaaactt ttcccctttg gtacatcaac tgttccattt acttcagcta
    42481 aagataaacc atttgtatgt tcaataagtc gatgcttatg aaattgttta ttggattgat
    42541 tattcaagaa aaaaccacct ttcctattta tctacgatta cattctacac ctcaaaaaga
    42601 aaaaattaaa gaaaaagatt cggtacgcct aatttttttt catggcgcgt cttgctagat
    42661 gacgaaagaa attttgttct cctctaaaaa aaccactatt ttttgcatga aactaaagaa
    42721 tacgcctttc ttttttcaat aaactttagt aaaataagtc attgagagca attcggaaat
    42781 agaaaggaga gatttatatg ttatcaacag gtattgtcgt acttattgct attattgctt
    42841 tgctaatcgg cgcagtaggt ggctttttcc tagcgcgtaa atatatgaaa gactatttag
    42901 aaaaaaatcc tcccgttaat gaggacatgt tgcgatcaat gatgatgtca atggggcaaa
    42961 aaccatctga gaagaaaatt cgccagatga tgcagcaaat gaagaaccaa gggaaaaaat
    43021 aggttctgac ccaacgactt tttacgggct gattttttca gtccgttttt tatttcgtaa
    43081 tttgttagac tattcagatt attcagcaaa aaagacaagc acagacaatc ctaggctctt
    43141 gataaagaca aaggaggagt aaaatgtcga tttttagaaa attaggctgg ttttttcgtc
    43201 aggaaaagaa acattattta attggggttt tctcgctatt cgcagtggcc ttggtacagt
    43261 tagttcctcc aaaagtgatt gggatcatca ttgatgagat cgccgataaa aatttatcaa
    43321 taaagatcat tttcgggtgg atcttcctat tgattatcgc tgctttttta caatatcttt
    43381 ttcgttatat ctggcggacg aatatttggg gaagtgcagc tcgattggag aaagaacttc
    43441 ggcagcagtt attcaaacat tttacagaaa tggacaatct cttttaccaa aaatatcgga
    43501 ctggggattt gatggctcac gcaacgaatg atttgaatgc gatccagaat gtagcaggag
    43561 ctggtatctt gacctttgca gattcagtga ttacgggcgg aacaacgatc attgcgatga
    43621 ttttgtttat tgattggcgc ttaacactga ttgcgctgat tcctctgcct ttgctggccg
    43681 tcacttcacg agtgctgggg tcaaaactcc atgatgcttt tcgtgattcg caagaagctt
    43741 tttctagtat caatgataag acacaagaaa gtatcactgg gatcaaagtc attaaaacat
    43801 ttggacaaga aaaagaagat ctagcggatt ttgaagaaaa gattgaagat gcaattatta
    43861 aaaacaaacg agtcaatttt ttagactcct tatttgatcc attcattacc ttgattatcg
    43921 gtctgtctta tgttgcaaca attattttag gtggaaagta tgtcttggaa ggcacgatct
    43981 cgatcgggca gatggtctca tttattagtt atatcggtat gcttgtttgg ccgatgtttg
    44041 cgattggtcg attgttcaac gtattggaaa gaggaaatgc ttcttatgat cgcgtcaatg
    44101 aacttttgca tgaaaagacg catatcgtgg aacaactggg tgcagtgcag accccggcaa
    44161 aaggcgagct gaatgtagct atttcctctt ttaattatcc gaaagaagaa tcaccagcat
    44221 tagaacaaat caagtttgcg atccaagaag gagaaacatt aggtattgtt gggaaaactg
    44281 gtgcgggaaa aacgacgatc atcaaattac tgatgcgtga atacgatgat tatcaaggga
    44341 aaatccaatt tggcggacgt tcgatcaaag aatattcatt gaatgcttta ttaggatcaa
    44401 tcggctatgt gcctcaggat cacttcttgt tttcgatgac cattcgagat aatatccgtt
    44461 tttctaatcc aaaactttca gaagaagaag tagagcatgc ggcttcttta gcatttatca
    44521 acaatgaaat caaggaatta ccaaaaggtt atgatacctt agttggagaa cgtggagtct
    44581 ctttatctgg cggacagaaa caaaggattt cgattgctcg agcattgatc aaaaatcctg
    44641 aattattaat attagatgat gcactctctg cagtagatgc caaaacagaa gaagctatct
    44701 tgcataattt aaaagaaata agaaaagaga aaacaactat cattgcagct caccgtttaa
    44761 gtagcgtgat gcatgcaaaa gaaatcattg ttgtcgatca agggaaaatc attgaacgag
    44821 gaacccatca tgagctgctt gcactaggcg gctggtatga acaaatgtgg gagaaacagc
    44881 aattagaagc gaagatagaa ggaggcgagg cctgatggat gaaaattatc aatcggaatg
    44941 gacaaaatcg attccactta aagaacagaa aaagatcatc aaacgactga tgaagttcgc
    45001 caaacctttt agaagaacgt tcatcgttgc tatcttattt gcgtttgctt tgtctgtgat
    45061 caatgtccta ttgccaagga tcatccaaac ttttatggat gatcatctgg ctaaacagtc
    45121 ggctacgaca caagttattt tgttttttgc tggtatgtac ttatttggag tcatagtaaa
    45181 aagtatcatt tggtttttcc aatggtattt gtattccatg gcctcattga agacctatca
    45241 gtatattagg gtaaaattat ttgaaaagct gcatacacta ggtatgcgtt attttgatca
    45301 gacaccggca ggatcaaccg tttctcgagt gacgaatgac acagaaacgc tatttgaatt
    45361 ttggtacgtc tttttgatgg tattgactgg gatctttgca gtcgtttctt cattccttgc
    45421 catgtttcaa atcaatggag aaattgcctt gtggactttg gtgtttttgc cgattttagg
    45481 aatcgtcatc tggtattacc agaaattcag ttctcgtatt taccgaaata tgcgggaaaa
    45541 attaagtcag ctgaacacga aattgaatga atcgatttcc ggcatgcgta ttatccagca
    45601 gtttcgccaa gaagctcgtt tagctaaaga atttgaacaa gtgaacaatg agtatttaga
    45661 cacgcgttat gcgatgatca aaacgaattc gattctgtta agtccgatca tcaattttct
    45721 atatgcattg gcgattgcgt tggtgttgac tttgtttggg atcgatgcgt tgcattctcc
    45781 cgtagaagtt ggactgatct atgcatttac gacctatgtc cagggatttt ttaatccaat
    45841 gtctcaaatg atggactttt tgagcatctt cacagatggg atggttgctg gtagtcgtat
    45901 cttgaagatt atggatacgg aagaatatgc accacaacaa aaccctggag cagatcaaat
    45961 gattacttca ggtaagattg agtttcgcaa tgtaagcttt tcttatgatg gagagcacaa
    46021 tgtattaaac aatatctctt ttgttgctaa tccgggagag accgttgcat tagtgggaca
    46081 caccggaagc ggaaaaagct cgatcatcaa cgtattgatg cggttttacg aattttatga
    46141 aggtcagata ttgattgatg atcatgacat tcgagaatat ccaatggaag agttacgtaa
    46201 gaaaatgggg ctggtcttgc aagatgcttt cttgttttat ggggatatcg caggaaatat
    46261 tcggatgatg aatccagcta tcacagacga acagatacgc catgcggctg agtttgttca
    46321 agcaaacaaa ttcatcgatg aattaccaaa aggatatcac tcaaaagtga tcgaacatgg
    46381 tgccagctat tctagtggac aacgacagct gatcactttt gcgcgaacaa tcgtgactga
    46441 tcctaaaatt ctagtattgg atgaagcaac agcaaatatc gatactgaaa cagaaggatt
    46501 gatccaagaa ggattggcaa aaatgcgtca aggacggaca acgattgcga ttgcccatcg
    46561 gctgtcaacg attcgagatg cagaattgat tttagtttta gacaaaggtc atatagtaga
    46621 acgcgggaat catgaaacac ttctagcgca aaatggtttg tatgcagata tgtacaaact
    46681 gcaaagcagt ggtatataaa agatgttgtg acagtagaat tttgacctaa gcactagagt
    46741 gaaaataaaa catagcttct catatttttc atctgtgaga gttagtgtcg tagtcaccag
    46801 atttaaaaaa agattcgaca gctagtcaga agtgcctaat agccactaag aaagcgttga
    46861 caaaagcatt tgctttcgat aatataaaga aaacgataca acttggatag tgattgcctg
    46921 atgcaagcta aacctgatga gtctcccatg gtttcatgga cggctttttg ggtttttctt
    46981 tttgttttgg atacagccct atacatcatg gaaaggagca aaacatgctg attgagaaag
    47041 tactgaataa caacgtcgta ataaccaggg atgaaagaaa tcaggaaatg atcgtgatgg
    47101 gaaaaggttt ggcattcaaa aagaaagtgg gtgacccgat ttttgactca tgcatcgaca
    47161 aaaaatttca gttgacaaat gacgggcttt cacaacagtt ccaagaattg gcacaagcga
    47221 ttcctcttga gtacttagaa atttcttgtg aaatcattga atatacaaaa gaggctctac
    47281 aagtccagtt gaatgattct gtgtatattg cattgacgga tcatttgtat acggctatcg
    47341 aacgtcacaa aatgggaata tttgtaccaa atgtattgct gtatgatatc aaacaactat
    47401 ttcctaaaga gtatgccatc gggaaaaaaa cagtgaagaa aatctcggat aaataccaca
    47461 tcaagttatc agaagatgaa gccggattta tcgcattgca tttagtcaat gcacaaggca
    47521 ttcaaagcca gcaagacaat acctacctgt ttactataac aatacaggat attctaaata
    47581 tcgtccgcta ttactataag attacatttg atgaagattc agtccatttt tttcgattcg
    47641 tcacacactt acgttttttt ctgacacgcg tcctagatgg agaaaaatca gaagtgggag
    47701 aagtttctga ggaattgttt caggttattc aaaaaaaata taaggatacg tttggttgcg
    47761 ttgagaaaat agcagacttt ttagccgaga cgttcaacta ccagatgtcc acggacgaaa
    47821 aaatgtattt aacgatccat actgcgcgtt tagtgcatga gaatcgttgt aaataaaaaa
    47881 aggaggataa attaacaaga acgtatgaaa taagggtagt gacattcagt tggctaaacc
    47941 ttccactaat aaattggagg gaatagaaat gggaaaatac caagaattag cgagaaaaat
    48001 cgtagaaaat gtcggcggga aggaaaatat caatggtttg acacattgta tcacaaggct
    48061 tcgtttcaaa ttaaaaaacg aagaaaaagc aaatgatgag atcttgaaaa atatggacgg
    48121 tattgtaacg gtaatgcgag cgggagggca gtatcaagtt gtcattggga atcatgtgcc
    48181 agtcgtgttt gaagaggtta taaaagcagg aaaccttact tttgatgagg ctgtttcaac
    48241 agaaaaaatg agaccgtttg atatgcttat tgatggaata tcgggctgtt tccagccatt
    48301 ccttgctatc ctagctgctg gcgggatgat tcgtggactg acagcattcc ttgtcttctt
    48361 aggcgcgttt gaccgcggtt ctggcacatt tgttatgttt gataatatcg gtgattctgt
    48421 attccaattt atgcctgtta ttatcggttt aacagctgcc cgtaaattta aagtaaatga
    48481 attcgtaggg atgttgcttg gcgcagcatt aatgaatcct agcttgtcgc tggaagcttt
    48541 gagtggtgca gcagaagcac cactaacgac catattttct ggcactattt ttgaagcacc
    48601 tatttatcaa acagtattcg gcattccctg gattgcgcgt aactacgctt cttctgtcat
    48661 tcccattatc tttattgtgt tgttagctag ccaaattcag aaaccaatta aaaagttagt
    48721 acctgagatg atcgcgaatt tcttcgttcc attcttcacc gttcttatta caatgccttt
    48781 aggtttttta ctcgttggac cagtattcac attcgcaact gatattttaa tggctggatt
    48841 tgaaacactt ttagcacttt cgccagttat ttatggagca atcgtaggct tcttctggca
    48901 aattctggtg atgtttggtc tccattgggc aatcgtacca atgggattga tgcaattttc
    48961 tgtgaatggt tggcaaaata ttatgacacc tgttgcagta gtgtcttttg gacaaactgc
    49021 agcacttacc gcactctact ttaagcttag aaatccaaaa gacaaagcaa ttgcaatacc
    49081 agccattgtt tctggtattg tggggattac agagccagct atttatgggt ttacattacc
    49141 acgtaaaaaa atatttattt ttacctgtgt cggtggtgcg ttagggggcg cgtatagtgg
    49201 gttaatgaat ttaacttctt ggaatcaagg tggactaggc atctttacaa ttcccaacta
    49261 tattagacca gatggtgatt taacagatgt gattaatgta ttgattggga tcgccattgc
    49321 gatggtggta tcctttacgc tgactttttt cttttggaag gatgaagcag gtgaaacaga
    49381 tgatatacag aaaaaatcag gtaaagaaat tgtaaaaaca ccgatacaag gacaaatcgc
    49441 tccactaaat gcagcaaagg atgcagcttt tgcacaagga acactaggtc gaggaatctt
    49501 gatctaccct gaaaaaggag aagtacgtgc cccttttgac ggaacagtca tgaccctgtt
    49561 cccgacgaaa caggcaattg gaatggtttc tgaaacaggg ctggaattgc tgattcatgt
    49621 cggattggat actgtgcagt tggaagggaa atattttgaa agcttagtgc agcaaggata
    49681 aagagtgaaa caaggcgatc tgttgctacg cttcgacaaa aagaaaatcg aaaaggaagg
    49741 atacagttta gaaacacctg tgatcgtcac caattacacg gattatttgg atattttgga
    49801 aaaccaaggg gagacggtcg ggccagacga tgcacttatc accgcactga cctgaacggt
    49861 taggtgaaga atggactagg cttaactatt ctttttattc cacttcttta ttttcaaaat
    49921 tgaaaggctc tttcatgatg gctgagctga tagggcgttt atcgaatttt tgaccgacag
    49981 aaattagatg gactaaattc tgttgataat tttctgcaaa aacacaacta tataacacgt
    50041 ctagaagata acaaattgat gtattgatcg taaaattacc aatttttgaa tacagtcgct
    50101 cgcgtgtcgt aagtcgcaaa gcggctgtac tcattgcaga tagcgttgaa tcaccgatac
    50161 tggtcaaagc taaaattgga acatgatttt gtttcaatat ttctgcggta cgaaggatca
    50221 cattgttttc acccgtataa gaaatcagaa tcccgcacat atcactcgtg caattgtacg
    50281 cttcatagaa tgattcacca aaagtggtga caatcgtgac atccattttg atgcgcttca
    50341 ttttcagcat gaaatcttga gaaatcaaag tattcgagtt actggcgaaa atcacgattt
    50401 tttttgcttg aagcattagt tgtttggctt tttgcagttg gtcatgatga atgagagaaa
    50461 gtgtatcatg aatcgtcatc tgttctaaag aagccatttt atttgcgatc gtcataatac
    50521 cgtctgtggt ggaaaaagga agattcgcat ctacgtcttg aaaattccct tcaagatagg
    50581 ctgtttcagc taaaaaagca ttacgaaaac tgacccagcc atcaaaaccc attttttttg
    50641 ctacacggat gagtgtagag gggtggacat aacatcgctc agcaatcgct tggatggtca
    50701 tttcgccaag tgctggagaa tcgcttaaga gaaactggat cacttcattt tctgaaggag
    50761 aaaaggagat ttctttgatt ttttcacgta ataacatgta gataccttct tcttattttt
    50821 ttacgtaaat tataactttt ttgaaaatga tttgtaaacg gatttatact atataactta
    50881 aaatagacat tttttgacaa gatatttaca aaaatccact agaaaacagt aaattttgta
    50941 aatgttactg gaaagaaaca gatttctatt gtttgttttg tttacaaaaa agataatgaa
    51001 agtgtaaaaa agtaaaggag tgagaacatg gcaaaaggaa tcaaaatcgc tacgattggt
    51061 ggaggcagca gttacacccc cgagttgatg gaaggattta tcaaaagata tgaggaatta
    51121 ccgatacgag aaatctggct cgtagatgta gaagaaggaa aagaaaagct tgagatcgtt
    51181 agtcagatgt ctcagcgtat gtgggatgct tctccgtatg aagtcaagat ctatgcaact
    51241 ctggatcggg aagaagcatt gaaagatgcg gattttgtta cgactcagtt tcgtgtcggt
    51301 caattagatg cacgaatcaa agacgagcgg atccctagtt attatgggat gattgggcaa
    51361 gaaacaaatg gtgctggcgg gatattcaaa gcttttcgta ccgttccagt cgttttggca
    51421 atcgttgagg acatgaagcg tttatgtcca aatgcttggc tgatcaattt cgcgaaccca
    51481 agtgggatgg tgacagaagc agttatcaga tatggcaaat gggataaagt gattggatta
    51541 tgcaatgtgc cagtgatggc gatgatgaca gaaccagcaa tgcttggcaa gaaaaaagaa
    51601 gagctgatct acaagtttgc cggtctaaat catttccatt ggcacaaagt agcagataat
    51661 aaaggaaatg accgcacggc agaattgatt gatcgtttat acgatgaaga gaatggatta
    51721 ccgaaaaata tctttgatat cccgtttttc aaagaacaat taaaacaaat gaacatgatc
    51781 ccttgtggct accaccgcta ttactatcgt gaagaagaaa tgctggaaca tgctttagaa
    51841 gaatatcaaa cgattggtac aagagcgcag caagtgaaag aaacagaagc agagctattt
    51901 gaattataca aagatccaga attagaccat aaacctgaac aattgcaaaa acgaggtggc
    51961 gcttattatt cagacgctgc ctgtgaaacg attgcttcaa tctatggaaa taaagaaaca
    52021 cagatcgtgg tatctacaaa gaatgatgga gctgttcctg acctgccggc agattgtgta
    52081 gtagaagtca ctgcctatgt tggcgcacaa ggagcgagaa atgtagcgtt tggttctctt
    52141 cctgctgctg aaagaggctg gttgcaagta atgaagaata tggaattgct gacgatcgaa
    52201 gcagctgtga ctggtgatta tgggacagct ttacaggcgt ttacgatcaa tccacttata
    52261 cgatcaggaa agacagcaaa acggatcatg gatgagttgt ttattgcaca ccagcagtat
    52321 ttaccgcaat tcaaggagac aatcagccga ttaaaagaag aaggaatcga agttcaagat
    52381 gaactggcaa gagaattatc aggtaataca catataatgg aggaagtata atgggattca
    52441 gaaaagattt tttatggggc ggtgctacag cagccaatca atgtgagggc ggttatgatg
    52501 aaggtggacg cggactagca aacgtagatg ttgtgccaat cggaaaagac cgttttcctg
    52561 ttatcaccgg aaagaaaaaa atgtttgaat ttgatgagga tcatttttat ccagcaaaaa
    52621 ttgcaatcga tatgtatcat cgttataaag aagatattgc tttgtttgca gaaatggggt
    52681 tcaagactta tcgaatgtcc atcgcctgga gtcgtatttt tccaatgggg gatgagaaag
    52741 agccgaatga agctggcttg aaattttatg aggatgcctt taaagaatgc caaaagcacg
    52801 gaattgagcc acttgtgacg attacgcatt ttgattgtcc aatgcatttg atcaaagaat
    52861 atggtgcgtg gcgttccaga gaactcgttg gtttttatga aaatctgtgt cgagtcatct
    52921 tcaatcgcta taaaggactt gtcaaatatt ggctgacatt caatgagatc aatatgatcc
    52981 ttcatgcacc attcatgggc gcagggctgt attttgaaaa aggagaaaat gaagaacaaa
    53041 tcaaatatca agcggcacat catgagttgg ttgcaagtgc catcgcaacg aagattgctc
    53101 atgaagtaga tccggagaac aaagtaggct gtatgctggc agcaggagca tattatccat
    53161 acacttgcaa tcctgaagat gtttgggctt ctagaaaatc agatcgagaa aattatttct
    53221 tcattgatgt ccagtcacga ggagaatatc ccgcttatgc attgaaagaa atggagcgaa
    53281 atggcattca gatcgaaatg gaaccagggg atgccgcatt gctgaaagaa catactgttg
    53341 attttatctc tttttcttat tatgcttccc gagtttcgac taccgacaac gaactcttgg
    53401 aacagactgc aggaaatatc tttgcttctg tcaaaaatcc atatctgcaa gcaagtgaat
    53461 gggggtggca aattgatcca ttaggcttga gaataacgat gaatgatatt tatgatcgtt
    53521 atcaaaaacc attgtttatt gtagaaaacg gcttaggtgc agtcgatacg ccagatgaaa
    53581 atggttatgt ggaagatgat taccgaatcg attacctggc agcgcatatc caggcgatga
    53641 aggatgcagt agaactagac ggagtcgatc tattaggcta tacgacttgg ggatgtattg
    53701 atttagtatc agctggtacc ggtgagatga agaaaaggta cggattcatt tatgtcgatc
    53761 gtgataacca aggaaatggc acattagccc gttcaaagaa aaaatcattc aactggtata
    53821 aaaaagtaat tgattcaaat ggagaaaatc tttcgaacga ttgatcaatg atttataaaa
    53881 gtctattgtt tttcagcgga tttttcgagt ataataaagc tattcgagaa acgcaagcag
    53941 aacaatagac tttttttcgc attggctgta aagtatgctt acttgattaa gtaaaggaga
    54001 atctacatat gtatgatgta atcgttattg gcgcaggtcc tgctgggatg actgctgctt
    54061 tatatgcttc acgttcaaac ttatccgtat tgatgatcga acaaggtgcc cctggcggcc
    54121 aaatgaacaa tacggcagaa atcgaaaatt atcctggatt cgattcgatc atgggccccg
    54181 atctagctta taagatgtat gaaagtgtct caagatttgg gacagaaaat gcttatggaa
    54241 tcgttcaaga tatcaaggat cacggcagcc ataaagaagt catctgtgaa gataaaagtt
    54301 acgaaggaaa aacagtcatt atcgcaacag gatgtatcca ccgtaaatta ggcgtacctg
    54361 gcgaggaaga atttgctgga cgaggcgttt cttactgtgc agtctgtgat ggtgcgtttt
    54421 tccgtaacaa acgtctagta gtcatcgggg gtggggactc agcagtagaa gaagccatct
    54481 atttgacaca atttgcttct gaagtagtga tcgtccaccg tagagatgaa ttacgtgcac
    54541 aaaagatcat ccaagaccgt gcatttgcaa atgaaaaaat ttcctttatg tgggatacag
    54601 tcgttgacga aatcgttgga aacgaaatgg tagtgacagg tgtgaaagca cgtaatgtca
    54661 aaaccggcaa tgaatttgat ttagcagcag acggagtatt catctatgtt ggactggatc
    54721 cattaactgc accttttaga gaagcaggtt tgacgaacga agctggctgg attccaacag
    54781 acaacgaaat gaaaacgaat atgccaggcg tttttgccat cggagatgtt cgtgaaaaag
    54841 atttgcgaca aatcactaca gctgttggtg acggcggtat tgctggacaa caagtgtata
    54901 aatacttgga gtctttagaa gaaacaacag cataagttta aaaagagtag aagaacgggt
    54961 tcaattgttc ttttactctt ttttttcgcg tgaaatttac tgaatctgat ttatgcgaac
    55021 acgtccaaca ccttttgata aaagaatcct ttcttgattt aagcgttttt tggtcctcat
    55081 actttcatat ctaataagtg aatgctatac ttattaagaa ccatatttca gaaagaggtg
    55141 ttattttcat gtcttgggaa caagtgtatg aacaatgggt acatgaagaa aatttagaag
    55201 aaaacttaaa aaaacaatta aatgatctgg gtgaagatcc tgaaaaactt gaagatgctt
    55261 tttatgcacc gttggaattc ggtactgcag gtatgcgggg aatcttagga cctgggatca
    55321 atcgaatgaa catctatacg gtaagacaag caacagaagg actagcacgt ttcatggata
    55381 cacaaggtga agaaacaaaa cgccgaggtg tcgctatcgc ttatgactca cgccatcagt
    55441 caccagaatt tgcaatggaa gcagccaaaa cactagcaca tcacaatatc ccgtcttatg
    55501 tattcgaaag tctgcgtcca acaccagagc tttcttttgc agttcgtcat ttgaagacgt
    55561 ttgcgggaat catggtaacc gcatctcaca acccagcggc ttacaatgga tataaagtct
    55621 acggagaaga tggcggacaa atgcctcctg cagacgcaga tgcattgacg aaatatgtcc
    55681 gtgaagtcag caatccgcta aaagtcgaag tattatctga cgaagaagct aaacatagcg
    55741 ggttgatcac gatcatcgga gaagaagtgg acgctgctta tctagaagaa atcaaagcag
    55801 tgacgattga tcgtgaattg gttgaaacaa tggggaaaga tttgaaactt gtgtacacgc
    55861 cacttcatgg aacagggaaa atgttgggcg aacgtgcgtt gaaacaagct ggattcgaac
    55921 aattcgtact agttcccgaa caagctgtgg cagatccaga tttcactacg gtcaaatcgc
    55981 caaatccaga agaacattca gcttttgaat atgctatacg acttggagaa aaagaaggag
    56041 ctgacttgct gattgcaaca gatcctgatg cagaccgttt aggcgcagcg gttcgtttgc
    56101 cagatggttc ttatgaagta ttgactggga accaaatcgg agcattgttt gctcaatata
    56161 ttttggaagc acatcaatca agaggcactt taccagaaaa cgcagcggta ttgaaatcaa
    56221 tcgtttccag cgaattgcct acagcaattg cggaacacta cggtgctacc atgttcaatg
    56281 tgttgacagg gtttaaattc attgctgaaa aaatccaaca atttgaagaa gaccattcac
    56341 atacttttat gtttggattc gaagaaagtt atggttattt agttaaacct tttgttcgcg
    56401 ataaagacgc gatccaagca ttgcttttat tagcagaagt agcagcttac tataaaaaac
    56461 aaggcaaaac gttgtatgac ggcttgcaaa cgatttttga aacctatggc tatttcgcag
    56521 aaaaaacgat ttctgtgaca atgagcggac aagaaggttc tgcgaaaatc tctgctttga
    56581 tggaaaaatt ccgtaaagaa gcaccgacag aatttgccgg agcaaaagtt attcaaacag
    56641 aagactttaa agaattgacg agaacagacc gtgaaggaaa cgtagaaaaa ctaacaacac
    56701 cgccatccga cgtattgaag tatacattgg aagaaaatgg ctggatcgct atccgccctt
    56761 caggaactga gccgaaaatc aagttttata ttggtgtaaa aggaagctct acagaagacg
    56821 cagccgaaaa agtagcctct tatgaagcga ttatcaaaaa gatgacagaa gaataaaaaa
    56881 cttgagactg atggaaaaag tggtaaactt tagaagtcag tcttttttca agttggaggt
    56941 gaatggattg ccagacacgc aaaaatcaaa agtagagaaa gtcagtcaat tattcaaaat
    57001 gttgagtgat cccactcgtc ttaaaattct gctatatctg aaagacgggg aacaaaatgt
    57061 gacagcaatc acccaagcag tggaaatgga gcagtctgct gtatcgcatc aactacgttt
    57121 attaagagaa aatcatgtcg tcaagtctca tcgcgaggga aaagcgattt tgtatagtct
    57181 tgatgattct catgtactgg atattttgaa ccagacactc aaacatgtgg aacatcaata
    57241 acgataggtg aaaatagata aacactggag tgtgacaacg aagaaatttc gttgtcacac
    57301 tccagtgttt tttaagaaaa agacaaaaat gattaatcgc ttttttctgt tgtttcagtt
    57361 ccataataac tatgatcttc taggtctaaa gcattcaaaa taagcgaagc cgtttcttca
    57421 atagataaag atgccacatt gatcactaca catcctagtt tttcataaag atcattcgca
    57481 aaatcgagtt cagctctaat tttttcgata tcggaataag cagtatcagg gtttaatccg
    57541 taagagcgca tccgttcttt acggatatta tttaaaatat ctgggttatt cgttagtccc
    57601 acgatttttt taggatcgat ttcccacagc tgtttgggaa tatgtgcttg gggaatcaaa
    57661 ggtaaattag caacttttaa atttttattt gctaaaaaca agctcaaagg cgttttggaa
    57721 gttcgggata cgcctagcaa aacgacatct gcttctaaaa aaccacgagg atctttccca
    57781 tcatcgtatt ttacagcaaa ttccattgct ttgatccgtt tgaaatagtt ctcattcaat
    57841 aaatggaggg caccaggttg ccgaattggc ttgataccag ttaaacgttc aatttctgat
    57901 atcggtggcg tcatcacatc gaaactaaat aattgatgtt cttcacaaaa agaacgtgtt
    57961 atctcgatca gttcttgatt gattaatgta tgcaggacaa gtgctttttc ttctaatgca
    58021 tcttgcaatg cctgccttaa tgttttttca tctgtcacga acgttctgcg gaataggttg
    58081 aactcaacgg taggatactg agccatagta gcttgcgcta gttttgtcgc agtttctcca
    58141 gcagagtcgg agatgacaaa aaaagtaacg atttctgtag aatcggaaga catggataaa
    58201 acctcacttg catttttttt atgtttatta taccatactc tagaatgcgt acttcgtaat
    58261 cattttgatt tggaggaata agtcatgaaa aaacagacat tgatcgaaca atcattagaa
    58321 acattgaaaa ataacggttt taaatatact aaaaaaagag aagcattgtt aacttattta
    58381 gtgaagcgta accgctacgt atctgctaaa gaagtttttg actatatgaa tcaggaattc
    58441 aaaggggtaa gttatgatac gatttaccgg aatctccatg attttgcaga gatcgattta
    58501 ctggaagaaa ccgatttaaa tggtgaaatg aagtttcgtt ttcattgctg tcagggggaa
    58561 atcggccatc accatcattt tatctgtaca gtgtgcggaa caacgaaaga aatccatatg
    58621 tgtccaatga attatttcga agaacagcta ggtggatgca cgatcgaagg gcatagattc
    58681 gagattttcg gaagatgtga aaattgtcaa taaggattta aataaaataa catttgataa
    58741 tttaaaaaaa aaagcaagat ttccttgacg gtcaaaacag cattagatat aatatttaca
    58801 ggacagtctt gttttttgag atatatttca ataacaatta cggttacttt ttaagctcgg
    58861 agggagggaa ttaacatgtc aaaaacagtc gttcgcaaaa acgaatcact tgacgatgct
    58921 cttcgtcgct tcaaacgttc cgtgtcaaaa gcgggtactt tacaagaatc tcgtaaacgt
    58981 gaattctacg aaaaaccaag tgtaagacgc aagaaaaaat ctgaagcagc tagaaaacgc
    59041 aaaaaattct agttctgtga tgaattggag ttggtagtgt gtcactacta agtagattaa
    59101 acgatgacat gaaagcagcg atgaaagcga aagataagga atctttacaa gtcatccgaa
    59161 tgatcaagtc atctatacaa aacgagcaga ttaaagaagg tcacgacttg acagaagaag
    59221 aagaactaac ggtgttgtct cgcgaaatga aacagcgacg tgattctttg catgagtttg
    59281 aagaagcagg acgtgacgat ttagctgaaa aagtaaaaag tgaaattgta atagtcgaaa
    59341 aatatatgcc ggaacaactt tccgacgaag aaattcgaca acttgttcaa gaagcaatca
    59401 ctcaaactgg tgcttcatca atgaaagaat ttggcaaagt tatgggtgca attatgccta
    59461 aagtcaaagg aaaagcagat ggaaaccaag tgaatgcgat tgtgaaagaa ctattacaat
    59521 cttaatttta tcatttaaac tgcggacagt gattgtccgc agttttttgt gttcgtaaaa
    59581 aaagaactaa aaaacagcta gaagaaagaa taagattctt tcttctagct gtttttttcc
    59641 tgtttaatat ctcttatgtt aaattgtgtt tgatttggtt attcttctgg tagttcattt
    59701 gcttcttgtt tataccctga taggcgatag gcgtaataaa agagtaagct gccagcgtaa
    59761 ccaagtaacg ttgggaccat ccagccaagt cctaatgcag caaaaggaac gtgatgatga
    59821 taaaatccta gtaagcttgc tacgacagtt tgctgactca ctaatgcagg cgaatttgcc
    59881 aacatatcca atacggctgg aacggcagaa aagaacatcg ttacttgata gacaaatgga
    59941 ctcttttgga agaattttgc tgttaaggaa agcaagatta acgcaagtga caatggatac
    60001 aaaagcatca atactggtaa agaccattgg atgatattat ccaatccggc attcgcaacg
    60061 acaaaagaga caaaagtcgt taaccgcagc catgtcatat aactgacttt agggaacagt
    60121 ttatggaaat cttgtgcaaa agaaacaacg agtcccatag cggtagtgaa gacacctaat
    60181 gtgaccatta cccctaagaa gctagcaccg aagttaccta aataaaaatt agtgatctga
    60241 gaaagtgcag ttccgccatt ttctgataaa gacatcttat tcaaactcat cgcaccgaga
    60301 aggaccagac caaaataaat catgacttct aacgcaacgc tcaggctacc agcttttgct
    60361 gttaattctg tgactttttt atctttaaaa ccaagatcac gaactgcatg gacaaaagtg
    60421 acacttaaag caagtaaagc tactgcatca accgtgttgt atccttctaa aacgccattt
    60481 gtcaaagcgt tcgtctgata agaatctgca gcagattggt ttagattccc catgggttgg
    60541 aataaagaaa cgacaaaaat gatagctaat aatgcaagaa acaaggagtt taaatacttt
    60601 ccgacatatt tgaccaaacg tgttggatgt acagtaagga aataagcaag taaaaagaaa
    60661 agagcagaga ataaaaacat acctaatgaa tcatattttt caggcaaaaa gggtttagca
    60721 gccatttcaa aagcagttgc tgctgtccgc ggtgtaccaa agaatggtcc gatcgttaaa
    60781 tgaacaagga tcaagaacaa ggcagcatac cattttccga caggtttagc taagtcatac
    60841 agaccatcac tttttgtcac tactacagct aagatagcta ataatgggaa aagagcgcct
    60901 gagatggcaa atcctacacc agcgctgatc cagtttgatc cagccatttg acctaagtgg
    60961 acaggaaaga tcagattacc tgacccgaag aacatcccga agataagtga actgaccatc
    61021 aataattgct tccatgaata agtttttgtt tcttccataa atttttcctc caataattat
    61081 cattttgagt aacaaaataa acgttgcaga aactagaaat gccactaata tcagattcca
    61141 tgaaaaccac caccttaaaa tataaaaaaa agcccttttc cacttgggaa aagggctcta
    61201 gattctagaa cggtaccacc ttttttcgct taaaaaagct tcttcattca cggacgcttt
    61261 tcgataaagt tatccgctag cctgataatg gaggcagcca caatgatcta cgttttcgac
    61321 attgactcgg aaatgatgtt cgtctattcg atttattgtc atttctcact tacatgactc
    61381 actgggaata aatcgctgaa tagcctactg ttttcctcga cgctttgctg tatatttatt
    61441 tgttgacgtt ataatacacc tgtttttttt gaaatgcaag aatatttcaa atattttgtt
    61501 ttcttttttt gaaacaagaa aaatacccag agaatacttg ctaagttaga cggaatactt
    61561 gtcctctatc ttatgcatag cttacatgat cagctttttt tattcaaacg agacctttag
    61621 agaatggaag cagctatttt ttaacgtttc ttggtactta tggttaagca actcttttag
    61681 aaccttttct tatttgctat atagtttctt ctgagtatgg taaaatagga aagaatgaat
    61741 cgaaaaaaag gggatgtcta tttttgacgg aagagtctag ttctttagag attagattga
    61801 cggctgctga tgatgtcagc atgcttttag gtgcacacga caagcatata aaactgattg
    61861 aagaaacaac agaaacaacc atccatacac gtggagaaat cattcagatc attggtgaac
    61921 aatcccagat ttccttggct gcctcagtca ttcggacatt gcaggaattg atcaaacgag
    61981 gaatccatgt aagtacacca gatgtcgtga cggcactgaa aatggggcaa aaaggcacat
    62041 tagattattt tgttgagatg tatgaggaag agatcgtcaa agaccgtaat ggaaaaccta
    62101 tacgggtaaa aaacagcggc caaaagaaat atgtcgaatc ggtagccaaa catgatatcg
    62161 tatttggcgt aggaccagcc gggacaggaa aaacttttct tgcagtagta ttagcaatcg
    62221 ctgccttgaa aaaaggtgaa gtgcaaaaaa tcattttgac gcggcctgct gtggaagcag
    62281 gggaaaatct aggctttctt cccggggatc taaaagaaaa agtagatcct tatctgcgtc
    62341 ctgtttatga tgctttatac caaatttttg gattagatca tacgaaccga ttgatggaac
    62401 gaggcgtgat cgaaattgct cctttagctt acatgcgggg gcggacattg gatgatgcat
    62461 tcgttatttt ggatgaagca cagaacacga cagttgctca gatgaaaatg ttcctgacac
    62521 gtcttggtta tcaatcaaaa atgattgtca atggagatac gagtcagatt gatctgcctc
    62581 gagggacaac aagtggtctt gttcatgcag aacgaacatt aaagcaaatc aagaaaatcg
    62641 attttgttaa ttttgaagca agtgacgttg ttcggcaccc tgtagttgcc gaaatcatca
    62701 aagcttatga aaaggcagat ctccatcaag aataagcttt gcttaggagg ataagcacat
    62761 gttgaataaa acactgcaga aactacaaga aaaacttgga aaggcagcat tgcctttcct
    62821 gctgttcttg ttttttatca ttatctgcgg cgttacattt agtagtgtca aacaaaaggc
    62881 caatgatttc agagaaggcc aagtagcaga agaaagtatc cgtgcaaaca aaacgatcga
    62941 gaacacggaa gaaaccgaac aaaaaagaaa actggcagca gaagcagtca ctccagaata
    63001 cacttaccaa aaagatcttg cagatgacca gaataatcgg atcaagcaac tatttgaatt
    63061 gatcgataag acgaatgatg ccatcaacaa atcttatgac gaaaagattg ataaggcaaa
    63121 ggaagatgaa aatatcccaa agcctactac agaagagcgg atcgccgcgt tgaagaaaaa
    63181 ttttgaaaat gtgaatgcag aaaatgttgc gttttatcaa aaactaccga acaattttta
    63241 ttcgacgata tttgaaatga ctgaaactga gatccatact gtgagggatg agagtctgaa
    63301 gctcattgac gaacagatga gcaaacaagt tcgtgaaagt gaattggaag cttttaaaca
    63361 agaagcagaa gagcaaatcc aatatttgaa tgtcacacca gcacaacaac aaatgattcg
    63421 ctacttagtg gatcaaggta tcgtagtgaa tgacgtattg aatgagaaaa agacagaaga
    63481 attgaaacaa tccgcacgag aagctgtaca accagtgatg atcttccaag gagaaatcat
    63541 tgttcgtgaa ggtaatcaaa tcgatgcttc agctatgaaa aaattggaat tactaggact
    63601 gactaatcaa acaacttcga tttttccgct aattgccatg gttttagctg ttttgcttca
    63661 aatcggcgta ctgatttata attcacttca attccaaaat gcaggagaaa gaacaaaata
    63721 cgtgcttttc tatgtgacag ctatgtcagt cagcatagtg ctgatgaaat ttttccaatt
    63781 attccaaacg gaacaagcta cttatatccc gctgttttat cccgctgctt ttgcaccact
    63841 tgttttaagc tttttcttga atcgaagagc agggatcatg gctggtattt tccaagcagt
    63901 atctgcactt ttcatttttt atgattcgat tggcacgaac tttctaacga ttatcttgat
    63961 gtcttatgtt ttctctgggc tcatggcaac gattgtcaga cgtaaacgga tctcagaaca
    64021 aggattatct gctgccatgt gggtcattat ttttcctgtg atgatggatg tcattttgat
    64081 cgtctatcaa ggaatgagct ttggtgatgc aacgacttgg ttaatgctcg tctgcgggtt
    64141 cactggtgca ctcttctcat tcttgctgac gatcggtcta cacccatata ttgaattgat
    64201 ggtcaatgat gacagtgtaa ttgtattaaa tgaactcagc aatccaaatc atcctttatt
    64261 gaaacagttg ctggaagaag cgccaggtac ttatcatcat agtatgatgg tagctaatct
    64321 aagtgctaac gctgtagcag aaatcggcgg acgttctttg ctaactcgag tagcttgcta
    64381 ttatcatgat atcggcaaga tcaaacatgc tagctttttc gttgagaact tgccttcagg
    64441 agcggaaaac ccgcacaatt tcttattgcc agaagacagt aaacatatta tctttggaca
    64501 tgtgacagat ggagccaaaa tattggaaga ttataatatg ccacaaatgg tgatcgatat
    64561 ttgcagacag catcatggaa ccacgctaat gcgttatttc tacatcaaag cgaaagaacg
    64621 caatccggaa gtaacagaag agcaattccg ttaccctgga ccaaaaccgc aaagtcgtga
    64681 agcagggatc gtcaatattg ctgacagttg tgaggctgca gtacgggcaa tggatcatcc
    64741 gacgagcgaa aaaatcgaaa gttttgtcca taatcttatc gaagagcgta tttccgatgg
    64801 tcaattagac gatagtggac tgaccttgaa agaaatccga aaagtggaaa aatcattgat
    64861 cagcggttta agtagcacct tccattctcg gatcaaatat cctaaaatga aaacagaagt
    64921 tgagaagata aaggaagaac aagaagaata gaaagaagcg atgtataatg gatattacct
    64981 ttatggatga aacaaaccaa gtctcagaag agaaaatgaa agaaatcgat gacttgctgc
    65041 aatttgcagc atcttattta aagctgccag aggaaacaga aatgtctgtt actttcatgg
    65101 acaatgcggc aatccaagta atcaatcgtg attaccgtgg aaaagatgca ccgacagacg
    65161 tcatcagttt tgctctggaa gaagaaggcg aagacgaaat cccagtgatt tttgaagatg
    65221 gagagaatcc attgcctcgt gtactaggag acatcatgat ctctattgaa cgtgctgctg
    65281 aacaagcaga agcttacggc cattcatatg atcgggaact tggcttttta gcggtacatg
    65341 gattcttgca tatcaacggg tatgaccata tgacaccaga agatgagaaa gaaatgtttg
    65401 gtttacagaa agagatcttg gatgcctatg gactcaaaag ataaaaacgt agagaaaaac
    65461 aaacatttcc tgatgtctgt ggaatttgct gtccaaggag tcaaaaccgt ttttgaggaa
    65521 gaacgcaata tgaggaaaca tgttgcgttc ggtatccttg ctttatttat gggcgtgatt
    65581 ttccagcttg atcggataga gtggttatgg ctgcttttag ctgtttttct cgtttggata
    65641 gtagagatca tcaatactgt ttttgaaaat gtagtggaca tgtttacgga ttttcatttt
    65701 catccgattg ggaaaaaaat caaagatatg gcagctggag cagttttagt gaccgcttgt
    65761 tttgctgtca tcattgggtt gattttattt gtccctaaaa tatatcattt gttgtttaat
    65821 taaaaaggag aacgtatatg ccagaacata aatcagggtt tgtcgcaatc gtcggccgcc
    65881 cgaatgtagg aaaatccaca ttattgaacc gaatcgtggg tcaaaaaatt gccattatga
    65941 gtgacaaggc acagacaact agaaataaaa tccaaggagt atatactaca ccagaagctc
    66001 agctgatttt tatcgataca ccaggaatcc ataaaccaaa gcatcgtcta ggagatttta
    66061 tggtagaagc agcttacagt gcgttgaaag aagtggatgc agtcttattt atgatcagtg
    66121 ctgatcaaaa acgcggtcgg ggagacgatt ttattattga acgtctaaaa aacgtgcatt
    66181 ctccagttta tctgattatc aataaaatcg ataagatcca tccagacgag ttgctgggaa
    66241 tcatcgaaga ttattcgacg cagatgcctt ttacacaagt gattccgatc tctgctacag
    66301 aaggaaacaa tgtcgaacgc ttaatggatg tattagtcag tgaaatgcca gagggcccac
    66361 aatattttcc agatgatcaa gtgactgatc atcctgaata cttcatcgtt tctgaattga
    66421 tccgtgagaa agtcttgttt ttaacacgag acgaagtgcc tcattctgtc gcagtcgttg
    66481 ttgattcgat gaagcgaaac gagaatgata aaatccatat ccaggccacg atcatcgtcg
    66541 aaagagatag ccaaaaagga atcattatcg gcaaaggcgg aaaaatgctg aagcaagtag
    66601 gaacgaaagc aagaaaggat atcgaaaatc ttttaggaga caaagtattt ctagaacttt
    66661 gggtcaaagt ccaaaaagac tggcgagaca aaaaagtcta tctgcaagac ttcggctacc
    66721 gaaaagatga atactgatat tctgaacttc ttataggtta agtatagaaa taaactggat
    66781 aaaaatccgc acgttgagaa aaacggcgga agatcgaaac ataaataatc tggatcggca
    66841 gaatctgctt ttccagattt attttttagg agggagaata tgagccagct tgcagaatcg
    66901 aaaggcttga ttttatctag ccgtaattac aaagaaaaag ataaattagt caaaatcttt
    66961 acggaatctt ctggcaaaat gatgttttat atcaaaggaa tccatcggca aaatcagcca
    67021 ttagctccgg ctttgctccc ctttaccgaa gcagtctata tcggtaattt tcgaagtgaa
    67081 ggtttgtcgt ttttaagcag tgtcaaagac gtccatgcgt ttcggaacat ccaagaagat
    67141 atctttttga gcggttatgg tacctatttg ctaaatctag tagatgcagc aatcgaagat
    67201 catcaatatg acccgcattt attccaattt acgcaacaag ctctgcaacg gatggatcaa
    67261 ggagatgatg cagaaatcat cacgaatatc tttgaagttc aaatactgca acgcttcgga
    67321 atcgcgccaa tttggacgca ctgtgtcgta tgcggggaaa caaaaggaaa atttgactat
    67381 tcttccaaat atggcggtgt gatatgcgag aaacactggc caatggacga acaccgttat
    67441 catgcagatc ctcgcgctgt ttatttcgtt cgaatgtttt cggctatttc ttatgataag
    67501 atcagtggaa tccacttgaa agaagaaacg aagcaggcaa tccgtgacct cattgaccag
    67561 ttgtatgatg aatatgtcgg tattcatcta aaaagcaaaa agttcattga tcaaatgaag
    67621 tcttgggcac acgtattaaa gacgccagag aaaaacatgc ctgaaaaaga gatacctgaa
    67681 aaagaagaat aaatgaaaga tacaacaaaa aaatcaaaac tatttgacaa atacagggac
    67741 ttccgtctat tattaaccgt agattaaata gattgttcac tgagaggatc tattatacaa
    67801 acaagaaaac tgcaaaatga atacgtacat tgaccaagat cagtaaaaaa cacgtggttt
    67861 agcgagttcg ggagagtgga agccggaccc aaacgatttt tgaagaggca cttgcgagta
    67921 cgataaatga agctgccgaa tctttattcg gaagtagggt ggaaccgcga aattattcgt
    67981 ccctatgtct gtaatcagac atagggactt tttgtcgttt tctttcctgt attcttattg
    68041 acagcaacgc ttaaaaattg aaaggggcaa aacagatgag taaaaaattg accgtccaag
    68101 atatgatttt aacattacaa aaattttggt catcaaacgg atgcatgctg atgcaagctt
    68161 atgatacaga aaaaggagca ggtacgatga gcccatatac attcttacgg gcaatcggac
    68221 cggaaccatg gaatgctgct tatgtagagc catcccgccg accagctgac gggcgttatg
    68281 gagaaaaccc aaaccgtttg tatcagcatc atcaatttca agtcgtaatg aaaccttcac
    68341 ctgaaaatat ccaagagctt tatttagaaa gcttgcgtct tttaggaatt gatccattag
    68401 aacacgatat tcgttttgtt gaagacaact gggagaaccc atcgatgggg tgtgccggtg
    68461 tgggttggga agtatggctt gatgggatgg agatcacaca attcacgtac ttccagcaag
    68521 tcggaggact accttgtaaa ccagttactt ctgagatcac ttatggacta gaacgtcttg
    68581 cttcatatat tcaagaagtt gaaagtgtct atgatctgga atgggctgat ggcgtgaaat
    68641 atggcgagat cttcaaacaa ccagaatatg aacattccaa atattctttt gaagtaagcg
    68701 accaagactt gctactaagt aattttgacc gttttgaaaa agaagctaaa cgctgtatcg
    68761 atgaaaatct tgttcatcca gcgtatgatt atattttgaa atgcagccat acattcaact
    68821 tattagatgc ccgaggtgca gtatccgtaa cagaacgagc aggttatctt tcccgtatcc
    68881 gcaacatggc tcgtgctgta gccaaaatct ttgtggcaga acgcgaaaaa ttaggattcc
    68941 ctttattgaa taaaaacaca gcaaatccag aaaacatgaa ggaggctgaa taagatggca
    69001 aaagacttat tacttgaaat cgggctggaa gaaatgcctg ctcatgttgt cacaccaagc
    69061 atgaaacaat tagaagaaaa aacagcaaaa tttttagatg aaaaccaatt gacttatgat
    69121 acgatcgaaa cattttccac accgcgccgt ttagcaatca aagtaacaaa tattccagaa
    69181 cgacaagcgg acagcgaaga agaagtcaaa ggaccagcca aaaaaattgc tttggacgca
    69241 gatggaaact ggacgaaagc agctgaagga tttgtccgag gacaaggtct gacgacagat
    69301 gctatcactt ttagagaatt aaaaggcgtg gaatatgtct atgtaacgaa atacactcac
    69361 ggcaaagaag caaaagaagt attgacaggt ttagcagatg tcattaaaag cctcactttc
    69421 ccagtaacga tgcattgggc agaatacgat tttgaatata tccgccctat tcactggatc
    69481 gtggcattgt tagatgacga agtaatccca ttcgaaatct tggatgtcaa aacaggcaaa
    69541 gcctctcgag gtcatcgttt cttaggcgaa gatgtaacat ttacgcatgc aaaagaatac
    69601 gaagaaaaat taaatgaaca atttgttatt gcttcaccgc aaaaaagaaa agaaatgatc
    69661 atcacacaag caaacgaatt agcagaaaaa aatcactgga tcattgatct agatgaagat
    69721 ttactagaag aagtcaacaa tctagtcgaa tatccaactg tcttttctgg cgacttcgaa
    69781 gaaaaatatc tatctgttcc tgaagaagta ctagtcactt ctatgaaaga acatcaacgt
    69841 tatttcgaag tacggaacga tcaaggtatg ttgcttcctc attttatttc tgctcgtaat
    69901 ggcgatcggg taaaattgga aaatgtggta cgaggcaatc aaaaagtatt gactgctcgt
    69961 ttagaagatg cggaattctt ttataatgaa gacaagaaag cttcaatcga ccaatacgtt
    70021 gaaaaattaa aaactgttac tttccatgaa aaaatcggta gcatctatga gaaaatgcaa
    70081 cgggtacaag tcatcgctcg catcataggc gaacaagtag gactatctga ggaagaattg
    70141 atggatctgc aacgcgctgc tgaaatctat aaatttgatc tagtaaccaa catggtagga
    70201 gaattccctg aactacaagg gatcatggga gaaaaatatg ctctgcttaa aggcgagaaa
    70261 ccagccgtag caacagcgat ccgtgaacat tacatgccta tatccagtga aggagatcta
    70321 cctgagacta cagtaggttc tgttttagcg attgctgata aattagatag tatcttttct
    70381 ttctttgcag ttgggatgat cccaagtggc tcaaacgatc catatgcttt gcgccgtcaa
    70441 gcatatggtg tcgttcgaat catcgaatcg aaaaaatggg cctttccttt gtctgttctg
    70501 caagaaacga ttagtgaagt tatcagtaaa gatacggatc gttttggcat tggcttgtca
    70561 gcgggacaac aacaggtgat cgactttatc aaaggccgtc tgcgccaatt attgacaact
    70621 aagaatattc gtcatgatgt catcgaagct gttttgaacg cagaacaaaa agatctgaca
    70681 aaagtatttg cagcagcaca attgttcaag cagcatcttg cagatgaaga cttcaaacca
    70741 tcgatggaag ctttgacacg tgtcgtgaac ttagcgaaaa aagcggagct tgaaaaacaa
    70801 tcagaagtag accctgaact gttcgaaaac gaagcggaga aagaactgca caaagcagta
    70861 gaggcaatga aaacaagctt tgctgaccaa acgatcaacg aaaattatga tcagcttgta
    70921 agccttcgcc cactgatcga aaattatttt gatcagacga tggtcatggc agaagaacca
    70981 gcagtacgta agaaccgatt aacgcaatta agtcaaattg ctgccatggc tttgtcacta
    71041 gcaagtttag atcaaatcat cacaaaataa tttctaatat gtaaataaac cagagaacag
    71101 gcacaaaata ggctatttct ctggtttatt
//
LOCUS       DAZUKC010000004        69597 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000004 DAZUKC010000000
VERSION     DAZUKC010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 69597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.004"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(40..483)
                     /locus_tag="ACG80T_000216"
     CDS             complement(40..483)
                     /locus_tag="ACG80T_000216"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4839901.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(499..1659)
                     /locus_tag="ACG80T_000217"
     CDS             complement(499..1659)
                     /locus_tag="ACG80T_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS4839902.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            2166..3866
                     /locus_tag="ACG80T_000218"
     CDS             2166..3866
                     /locus_tag="ACG80T_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4839903.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(3968..4801)
                     /locus_tag="ACG80T_000219"
     CDS             complement(3968..4801)
                     /locus_tag="ACG80T_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFS4839904.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(5105..7270)
                     /locus_tag="ACG80T_000220"
     CDS             complement(5105..7270)
                     /locus_tag="ACG80T_000220"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFS4839905.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKNPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            7530..9824
                     /locus_tag="ACG80T_000221"
     CDS             7530..9824
                     /locus_tag="ACG80T_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFS4839906.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            9817..10482
                     /gene="pgmB"
                     /locus_tag="ACG80T_000222"
     CDS             9817..10482
                     /gene="pgmB"
                     /locus_tag="ACG80T_000222"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFS4839907.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            10475..11482
                     /locus_tag="ACG80T_000223"
     CDS             10475..11482
                     /locus_tag="ACG80T_000223"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFS4839908.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            11504..12523
                     /locus_tag="ACG80T_000224"
     CDS             11504..12523
                     /locus_tag="ACG80T_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4839909.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(12804..14171)
                     /gene="nhaC"
                     /locus_tag="ACG80T_000225"
     CDS             complement(12804..14171)
                     /gene="nhaC"
                     /locus_tag="ACG80T_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFS4839910.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(14280..15701)
                     /gene="tyrP"
                     /locus_tag="ACG80T_000226"
     CDS             complement(14280..15701)
                     /gene="tyrP"
                     /locus_tag="ACG80T_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFS4839911.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(15944..17821)
                     /gene="tdc"
                     /locus_tag="ACG80T_000227"
     CDS             complement(15944..17821)
                     /gene="tdc"
                     /locus_tag="ACG80T_000227"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFS4839912.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRNVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(18112..19368)
                     /gene="tyrS"
                     /locus_tag="ACG80T_000228"
     CDS             complement(18112..19368)
                     /gene="tyrS"
                     /locus_tag="ACG80T_000228"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFS4839913.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(19480..19685)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            19948..21249
                     /locus_tag="ACG80T_000229"
     CDS             19948..21249
                     /locus_tag="ACG80T_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFS4839914.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(21318..21917)
                     /locus_tag="ACG80T_000230"
     CDS             complement(21318..21917)
                     /locus_tag="ACG80T_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4839915.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            22171..23052
                     /locus_tag="ACG80T_000231"
     CDS             22171..23052
                     /locus_tag="ACG80T_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS4839916.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            23065..24675
                     /locus_tag="ACG80T_000232"
     CDS             23065..24675
                     /locus_tag="ACG80T_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4839917.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(24752..25411)
                     /locus_tag="ACG80T_000233"
     CDS             complement(24752..25411)
                     /locus_tag="ACG80T_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS4839918.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(25694..26359)
                     /locus_tag="ACG80T_000234"
     CDS             complement(25694..26359)
                     /locus_tag="ACG80T_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFS4839919.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(26506..27111)
                     /gene="dhaL"
                     /locus_tag="ACG80T_000235"
     CDS             complement(26506..27111)
                     /gene="dhaL"
                     /locus_tag="ACG80T_000235"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFS4839920.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(27139..28149)
                     /gene="dhaK"
                     /locus_tag="ACG80T_000236"
     CDS             complement(27139..28149)
                     /gene="dhaK"
                     /locus_tag="ACG80T_000236"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFS4839921.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(28124..28519)
                     /gene="dhaM"
                     /locus_tag="ACG80T_000237"
     CDS             complement(28124..28519)
                     /gene="dhaM"
                     /locus_tag="ACG80T_000237"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFS4839922.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            28673..28993
                     /locus_tag="ACG80T_000238"
     CDS             28673..28993
                     /locus_tag="ACG80T_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFS4839923.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(29105..29962)
                     /locus_tag="ACG80T_000239"
     CDS             complement(29105..29962)
                     /locus_tag="ACG80T_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFS4839924.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(30070..31374)
                     /locus_tag="ACG80T_000240"
     CDS             complement(30070..31374)
                     /locus_tag="ACG80T_000240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4839925.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(31371..31880)
                     /locus_tag="ACG80T_000241"
     CDS             complement(31371..31880)
                     /locus_tag="ACG80T_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4839926.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(32055..34190)
                     /locus_tag="ACG80T_000242"
     CDS             complement(32055..34190)
                     /locus_tag="ACG80T_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFS4839927.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(34273..35928)
                     /locus_tag="ACG80T_000243"
     CDS             complement(34273..35928)
                     /locus_tag="ACG80T_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4839928.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(35930..36244)
                     /locus_tag="ACG80T_000244"
     CDS             complement(35930..36244)
                     /locus_tag="ACG80T_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS4839929.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            36414..36674
                     /locus_tag="ACG80T_000245"
     CDS             36414..36674
                     /locus_tag="ACG80T_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFS4839930.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            36688..37002
                     /locus_tag="ACG80T_000246"
     CDS             36688..37002
                     /locus_tag="ACG80T_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFS4839931.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(37732..38286)
                     /locus_tag="ACG80T_000247"
     CDS             complement(37732..38286)
                     /locus_tag="ACG80T_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFS4839932.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(38412..40341)
                     /locus_tag="ACG80T_000248"
                     /pseudo
     CDS             complement(38412..40341)
                     /locus_tag="ACG80T_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(40647..41363)
                     /locus_tag="ACG80T_000249"
     CDS             complement(40647..41363)
                     /locus_tag="ACG80T_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFS4839933.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(41364..43187)
                     /gene="glpO"
                     /locus_tag="ACG80T_000250"
     CDS             complement(41364..43187)
                     /gene="glpO"
                     /locus_tag="ACG80T_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFS4839934.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(43201..44697)
                     /gene="glpK"
                     /locus_tag="ACG80T_000251"
     CDS             complement(43201..44697)
                     /gene="glpK"
                     /locus_tag="ACG80T_000251"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFS4839935.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(44833..46293)
                     /locus_tag="ACG80T_000252"
     CDS             complement(44833..46293)
                     /locus_tag="ACG80T_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4839936.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(46283..47578)
                     /locus_tag="ACG80T_000253"
     CDS             complement(46283..47578)
                     /locus_tag="ACG80T_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4839937.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(47910..48587)
                     /locus_tag="ACG80T_000254"
     CDS             complement(47910..48587)
                     /locus_tag="ACG80T_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFS4839938.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(48617..49708)
                     /locus_tag="ACG80T_000255"
     CDS             complement(48617..49708)
                     /locus_tag="ACG80T_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFS4839939.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(49809..50243)
                     /locus_tag="ACG80T_000256"
     CDS             complement(49809..50243)
                     /locus_tag="ACG80T_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFS4839940.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(50827..52284)
                     /locus_tag="ACG80T_000257"
     CDS             complement(50827..52284)
                     /locus_tag="ACG80T_000257"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4839941.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(52281..54176)
                     /locus_tag="ACG80T_000258"
     CDS             complement(52281..54176)
                     /locus_tag="ACG80T_000258"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS4839942.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(54234..56009)
                     /locus_tag="ACG80T_000259"
     CDS             complement(54234..56009)
                     /locus_tag="ACG80T_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFS4839943.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(56059..57555)
                     /locus_tag="ACG80T_000260"
     CDS             complement(56059..57555)
                     /locus_tag="ACG80T_000260"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4839944.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(57749..59371)
                     /locus_tag="ACG80T_000261"
     CDS             complement(57749..59371)
                     /locus_tag="ACG80T_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFS4839945.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(59699..61012)
                     /locus_tag="ACG80T_000262"
     CDS             complement(59699..61012)
                     /locus_tag="ACG80T_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFS4839946.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(61257..61481)
                     /locus_tag="ACG80T_000263"
     CDS             complement(61257..61481)
                     /locus_tag="ACG80T_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4839947.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(61501..61923)
                     /locus_tag="ACG80T_000264"
     CDS             complement(61501..61923)
                     /locus_tag="ACG80T_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4839948.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(62065..62775)
                     /locus_tag="ACG80T_000265"
     CDS             complement(62065..62775)
                     /locus_tag="ACG80T_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFS4839949.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(62793..63290)
                     /locus_tag="ACG80T_000266"
     CDS             complement(62793..63290)
                     /locus_tag="ACG80T_000266"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4839950.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(63327..63779)
                     /locus_tag="ACG80T_000267"
     CDS             complement(63327..63779)
                     /locus_tag="ACG80T_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS4839951.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(63914..64519)
                     /locus_tag="ACG80T_000268"
     CDS             complement(63914..64519)
                     /locus_tag="ACG80T_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFS4839952.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(64969..65589)
                     /locus_tag="ACG80T_000269"
     CDS             complement(64969..65589)
                     /locus_tag="ACG80T_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4839953.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(65591..66460)
                     /locus_tag="ACG80T_000270"
     CDS             complement(65591..66460)
                     /locus_tag="ACG80T_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFS4839954.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(66483..66890)
                     /locus_tag="ACG80T_000271"
     CDS             complement(66483..66890)
                     /locus_tag="ACG80T_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFS4839955.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(66909..68198)
                     /locus_tag="ACG80T_000272"
     CDS             complement(66909..68198)
                     /locus_tag="ACG80T_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4839956.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            68256..69595
                     /locus_tag="ACG80T_000273"
     CDS             join(68256..68697,68697..69595)
                     /locus_tag="ACG80T_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFS4839957.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT        20201 a        14624 c        12073 g        22699 t
ORIGIN      
        1 aaaaattagg ctgggagcat tatttcgtta ttctgttcat caaaaatcag cgttttgatt
       61 ccatttcatc acaagttcct cctcatttac tgcttcatct ttttgctgtg tttgaattgt
      121 aaaaccttgt gaaagataga aatgaatagc tgattgattt ttttgataga ctgtcaaagt
      181 cagtgtttta ttggtttctt ttattttttt cattagttgc gtaccgattc tttgttgccg
      241 gtattccctt ttcacaaaga ttcctgcgat aaactgatca atcaccccag caaaaccaat
      301 aatttcttga tctatttctg ctaagtaaat cactgcatct ggtaaagctt tcctaacaga
      361 ttctacttga ttctcccaat aagttgatgg aatgaattca tgagaatcca agttagcatg
      421 cagccaaatc gatagaacag attccagttc tttggcagac aattttgttg ttgtgcggat
      481 cattttttct ctccttactt attcattgat tttaaaataa aaaccatttt tttgaagctc
      541 ttttacaagt tttcgcccct caaaaaagta tatcttctct tgttttactt ttctaatacc
      601 aaaagaaaca gcaactttag gatcatcagc tgaaacgatt cgataagttc caccaggcaa
      661 ttgatccagc ttgactttcc catgatggtc gctttgtact gaaaacggca caccatttct
      721 agtaagtgtt tttttcttat gtttcaaaat gaatgaacta ccatttaccg gtttccctga
      781 cgaatcacta ataataaatg aaaaatcaag cactttttta cttaatctat actgatagat
      841 tagtcgtccg ataaacacta acagtatgac tacagctatt atcgttcctg caagaataaa
      901 ccattgccgc atggttcttt cctgatttcc ttttccttct tcttttttta ccgattcgtt
      961 ataaggaatc cgatgtcctg tcactagcaa acgatgcgtg ttgatcatat acggcgtaca
     1021 tgtaagcaag gtaactaagt cttctccttc tgaaatcgtt agaacagacg tgtcatctgg
     1081 cagtaccact tgtatatttt taacttgata agccaatttc tcccccaaaa cagtcaaaat
     1141 aaacgtgtct ccgttttcta aacgatctaa ttcggtaaat aaccgtcgat taggtaaacc
     1201 gctgtgagca gagatcactg aatgcgtcga atttccacca agcgggaaag aggttccttg
     1261 caagacgcct gctccatttt caaggatcct accattgagc cgatcataaa gtgaaatatt
     1321 taattggatc ttaggtatat tgatacttcc gatcagccat tcctctaatt ggctggatgt
     1381 tgtactttca tttctgttct tttcattgaa aggatcagta ccaggattga atccttggtc
     1441 agttaatcgt tggtttttct cctccatttt ctttcgctgc acttcaattt cagctgcggt
     1501 ccgtttttgt acttgttcga tacgtttttg atcaataaaa ctatttaaag aatccacata
     1561 aaatggatat aaagcgatca gtaaccctac gacgatacat attaataaaa acaaacgaaa
     1621 caaaagtcga cgagactgtt tttgcttact ctgcttcaca tgtgtcctcc cttgcaagtt
     1681 tttttgttct ttcttttatt atatgggttc aggaaaaaaa tagaaaatga aagccgatga
     1741 ggttgtgaaa aagctgtcct gcatcaagta ataagtacag cccaaaaaaa tagctactca
     1801 tattttttgt tgggttgggc ttattaccgc agatgcaagc ttttgaacac cgtttaagga
     1861 agaggttgtg aaaactgcgt tatgctttga gtaataagag cgctagtcag aaaatagctt
     1921 ctcatatttt ctaggctagt gagcttatta ccgagaagca gctgattgaa caccgtttac
     1981 cgagaggtca tgaaaaagct actttgtttc gagtattaag aaaaattctg tttcaaaaca
     2041 aggagatgca acaaaactta tgtcgcatcc tctctcctta gaactttcac ttatgttttt
     2101 cttgtaaaat tcataaatat tttcatgaat tttctaattt cttgcctacc tctcgcatag
     2161 gatatatgat gaatttatct aaagtttgca aaggagttcg acaattgaaa aaatatggcc
     2221 catttttagc aatttttttc attgctttgg catttctcat gcctcattta ttcacacata
     2281 aactaatttt gggtgctgat gccccattcc attacaatcg gttctatgat gccgcgcaac
     2341 aaattaaata tggggatttc cattattttc cttcccttta tggattccag caaagtggca
     2401 ggatcgtgaa tgccgtttac ggcccatttt tcgcttattt acaaggattg atcgtgttgc
     2461 ttgcaggtag ctggtttcat tatcagattc tttcaaattt cattctttat cttatttcag
     2521 gattttctat gctgatttta ctgagatatg tcaatgttcg aaattgggta agtgtaccag
     2581 tagccgtttt attcatgagt acgtattcta ttcaatactg gtctatcaac caaggattca
     2641 ctagctgggg gacttgtttt tatcctttat gtatgatccc tattgctgat ttcgtccttc
     2701 ataaaaagtt ccctgttatc aaagtcgctg tctcagtggg acttatgaca cagatccatt
     2761 tactgtcttc gatcatgtta gctttgatgt atatcccttt ttatctttat tactttttta
     2821 accagactga aaaaggaaaa gcactgcttc aactgattaa gtcggtcttg ctttattttg
     2881 ttctgacagc aaatatttgg gcaggattag ctattgttta cagtgggaat aagattcttc
     2941 ctccctttgt taacagagaa atgagtcaaa aggcgataga tctggatggc agttattggc
     3001 tccattaccc taaggccttt cctatattga tattatcagg ttttttgctt tttattttct
     3061 ttcaacaaaa gaacacctta ttccaaaaaa tcgtgttgta cacaagtctt gttttctttt
     3121 ttctttctac tagcttgatt ccatggacga cattgatcga aaaaaatgtg ccttttatta
     3181 gtttgatcca gtttccattt cgatttttcg tgccatttac gctattgctt ctactttacc
     3241 ttgccctttc actgaaccag tggtctgaac aaaagtggtt ccgtctttta agtatatttg
     3301 gcattcttat tttttcagca cagacgatcc agaacttata ccagcatctg gaaaagtggg
     3361 aaaatgaaac atttgtcagt cgacatactt atctattcga tacgccggaa gaagcaagga
     3421 aaacattctt cagcaaagat atgtctacca gcttgttcgt tttccaaaaa accacacctg
     3481 actatctgcc aatctataag gagacaaaag acaataaata cgaccggtat aacgaagaaa
     3541 tactagaaaa cgaaacaact tttataaaaa cacatccagg cggtcttttg acaatcaagt
     3601 ggaaaaacac tggggacaaa actgttcata tccctgttat cgtctacgat cggactgtgc
     3661 tacagcaaaa tggaaaaaca ttgactgatt atgaagtaac agatatcggg accccaatcg
     3721 tgaagcagca aaaaggaatc aacgagctca cgcttcatta tcaaacgcca atctatttct
     3781 atttcattct ttctcttact ttgattggct ggtttacctt gctatgttta tttatttatc
     3841 atagatacaa gcttttacgt gcttaggcaa aagggataaa tacatccgat caaaaaactc
     3901 tccaaacggt ataatcgttt ggagagtttt attttaatca gtttttttat gttatatgtt
     3961 atatttttta ttcgttcaca tctaattcga tttccacccc gtagtgatca ctgatgattg
     4021 gactatcgat cccatcaaaa atccgttgat attttttaac ctgcattcct tcagggacga
     4081 aaataaaatc tatacgcaat ttttctgtat tttgttccca accatctatt ttcttttcca
     4141 cagtagctgc ttttcctttt tcattcgcga caacatacga atcttgaaga agaaggcaac
     4201 tttctcttac agtataataa ccttgagagt ctactggatt attgaaatca cctaggaaaa
     4261 aaagtggtac gcgagtttcc aaaaagtagt tttccaagtt tttccactca aaataaaagc
     4321 cacttttttt atctgtccac caagaaaagt ggcagctcga gacaaaaatg tcctgatcat
     4381 ctatctttgt tttcccaatc aagatcttgc gtgtctgata atcttctttc ttttgaaatt
     4441 gggagacggt aaggacctga ctttccaatg tttttttcgt aagcagtgca tttccttctt
     4501 catatttatc ataaccgatg tggctcattt cccaactcca aaaatactct tttccgtgtt
     4561 cctttaaaag ctgaactaaa cggtaggcaa aattatcttt acggataggt acttgattct
     4621 ttaccggaca gaacttcatt agcttcgcag gttctacttc ttcagattcc agtaattggt
     4681 tgatttcttg taaagcaatc acttcataat cttctaacag gatcttatct gctaattctt
     4741 gcaatttttt ctctggttct ggctcaagcc agctatgcgt gttgagtgtc atgaatttca
     4801 ttggttttct ccttgtcttc atttcgaaac aaaagggttg tgacaaaatt tttgtcacaa
     4861 cctctccagc aaatagtgtt ctcctgctaa ctcctcagta ataagctcaa tggaccagga
     4921 gctatttttt gtttggctgt ttttatcact tggtgcaggg cagctttttc acgacctctc
     4981 cactaaacag tattcaaact gctgcttctc ggtaataagc ccaaccaaac gaaaaatagg
     5041 aggagctatt tttgtttggc tgttcttatg acttgatgca ggacagcttt ttcacaacct
     5101 ctccttacat tttaatggag ccgatcactt taccagctgt cactgtacct aattgcttga
     5161 tcactacttc ttctacttta tcgtttgtaa atacgatcat gatcgttgga tctttttctt
     5221 cgttttggat cacatcaaga tccatctcag ctaatttcgt tcctgttcct acttcttggc
     5281 cttctttgac agcaatctca aaagctggct gattcatttg gactgtatcg attcccatat
     5341 ggatcaaaac ttcgatacca tctttcgttt ccagcccaat tgcatgtttg ctagggaaaa
     5401 tacttgtcac ttttccagcg atcggcgcaa caacttctct tgatgttgga ataacagcaa
     5461 agccgtcacc catcattttt tgtgagaata ccggattttt tacttcagat aaaggaatca
     5521 cttctccaga agctgcggaa actagttctt gttctatacc taagtattgc ttactacctg
     5581 cttgtccatt tcctgtagaa accatttctg gctctggaat ctctgctcct gattgcagca
     5641 gatcttcgat atctgatttc aagacatctg cttttggtcc ataaactgct tggactccat
     5701 tgtctttgac gatcaatccc atcgcccctg ctcgtttcca ggcttcttca gtccctactt
     5761 tttctcgatc atttacactt acgcgtaacc gagtcataca agcatcgaca tctttgatat
     5821 tttcttttcc tcccagcaaa tgaatgatct ggaccacttg ctgatctgct gttcctgttc
     5881 cgctgttaga tacaacggtt tcttcactgt cattgtcgta atttccgtta cgtcccggtg
     5941 tagcaaagtt gaattttttg atcaagaaat ttgctaagaa atacgtaaca acaccaaaag
     6001 cgatcacaca caacacgaag ttgataagat ctccacctag acccgctttg atagctaacg
     6061 gtgtccgagt cagcaattcg atattcccaa atgaatggac ccgcaatggc aagatatctg
     6121 ccatcgcaaa tgcagcccct tggatcactg aataaatgac atataatggc acagcagcaa
     6181 acataaacat gaactccaat ggttctgtta cacctgttag aaagacagcc aacgctgctg
     6241 aaaaatacat tgatttatat cttgctttct tatcaggatc gacattacga tacatcgcaa
     6301 aagccattcc catcaagata cctgaagaac caatcatttg tcctactttg aaacgagccg
     6361 gcgtccagtt atgcaacaca aactcatatt gcgacatatc ccctgcacct ttaagattca
     6421 ctaaatcagt ggcccaagcc aaccataacg gatcttgccc aaatacttgt gtccccgcct
     6481 gtgcgcctga taaaatctca tacgatccgc caagctgtgt atagttgata ggaatcgtca
     6541 gcatatgatg caatccaaat ggcaatagta aacgttctaa tgttccaaat aaaaacggtg
     6601 ctagaatcgg tgcgctttct tgtgattctg caatccataa accaaaatta ttgattccag
     6661 cctgtacgtt tggccacacc acagcaagaa tcaatgccac gattgttgac cataagataa
     6721 caacaaacgg aacaaagcgc tttccattga aaaatgacaa agcatccggc aattttctat
     6781 aattatagta cttattataa gccattgccc cgacaaatcc agcgatgatc cctacgaata
     6841 ctcccatatt caaagctggt gcttccaata cacttgtaaa aaatccttta accatgatct
     6901 tcgtaccgaa cagagtgtgg gtaaatgcat cttcattcgc aagcatgtca cttgtgacac
     6961 caaagatagc cccagtgatc cgattgatta aaataaatga gatcccagct gcaaatgctc
     7021 cgcctgctct ttcttttgcc caacttcctc ctattgctaa agcaaataaa atatgaagat
     7081 taccaataat tgcccaacca atattttcaa ttactccgcc cgttgtgaca agcggcgtcc
     7141 aatcaggatt gatcaaggga attgatttcc ctatactgat catcaaccca gctgccggca
     7201 taactgctac cacaaccatt aaagcctttc caaatttttg ccaaaactca aaactaaata
     7261 tctttttcat tccgtttagc tcctttacgc aatcggtttc gttaaacgta ttatagcgtg
     7321 cgttttcttt tttgtaaatg ctttattttt tatttttctg tctataccat tctataaaac
     7381 ctataatacg tgtgttccct tctataattt tctcctaaaa atttattttt ttaagctttt
     7441 ttctatgaaa tattttcttt tgtctattga cttgtgcaat cggttgcgtt tattatgaac
     7501 gtataaaaca gctcattagg aggagaaaaa tgaaacagat caaacgacta tttgctattg
     7561 atccatggaa aatcagcacg acacaattag ataaaaaaaa tcttcgactg caagaatctt
     7621 taacaagtat tggaaacggt tatatgggga tgcgcgggaa ctttgaagaa acttactcag
     7681 gggaccacca tcaaggtact tatttggcgg gtgtctggta tcccgataag acacgtgtcg
     7741 gctggtggaa aaatggttat ccagaatact ttggaaaagt gatcaacgcc ttgaacttta
     7801 tcgcgataga tatttcgatc aacgatcagc tgatcgactt ggcttctttg gaaccagaag
     7861 acttttactg ggaattagat atgaaaaatg gtgtattatc tcgtacattc acaatcacta
     7921 cttctactaa taaagtacgc atttccttcg aacgttttct gagcattgtc aaaaaagaat
     7981 cagcttatat ctatttaact atagaaatgc ttgaaggtag cggaacagtc aaactcgtat
     8041 caaaattgga tggaaatgtt caaaacgaag acagcaacta tgatgaacat ttttggaatg
     8101 agattgatcg tggaactata gaagaaatcg gctatttgac gactaaaact attccaaaca
     8161 actttgggat tgaacaattt actgttactg cagcaatgcg ccatttcgtt aatggaaaat
     8221 cagtcatccc tgactatcac gaagatactt tagcattaca tggttgcttg tctgcagaat
     8281 tatctcaagg tgagacatgg tcagtggaaa aagaaattat cgttcttact agtagagata
     8341 ttccagaagc gcagcaagca gaaaaagctg tctcagaatt aacgaaccag acaaacaatt
     8401 atatccaagc aaaagaagag caaacagcgg cttgggcaga acgatggaag cttgccgatg
     8461 tggtcatcga aggagacgaa gaagcacaac aaggtattcg attcaatttg ttccaactat
     8521 tctctactta ctacggcgaa gatgaacgat tgaatatcgg ccccaaagga ttcacaggag
     8581 aaaaatacgg cggagccact tactgggata cagaagcgta tgctgtccct ctttatcttg
     8641 ctttagccaa acctgaagta actaagaatc tgctgaaata tcggcataat caactgccgc
     8701 aagcaatcca caacgctcag caacaaggat taaaaggcgc gctttatcct atggtcacct
     8761 tcactggtgt cgaatgccat aacgagtggg aaatcacatt cgaggagatt caccgaaatg
     8821 gcgcgattgc ctatgcaatc tacaattata cgaattacac cggcgatacg gaatatttga
     8881 aaaatgaagg actggaagtt ttagcagaaa tcgcacgctt ctgggcagac cgtgtccatt
     8941 tttcaaaacg agcaaataaa tacatgatcc acggtgtgac cggcccaaac gaatatgaga
     9001 acaatatcaa taacaattgg tatacaaata cgatcgcagc ttgggtcttg cgttacacgc
     9061 gagaaaatta tttaacttat caaaaagaag ctgctatcac tatcagcgaa gaagaactag
     9121 ccaaatggaa agatatcgca gagaacatgt acttcccagt tgacgaagaa ttaggaatct
     9181 ttgtccagca tgacacattc ttagataaag atctcatgcc tgtatccgat attcccacaa
     9241 gcgatcttcc tctgaatcag cattggtctt gggacaaaat ccttagatct tgcttcatca
     9301 agcaggcaga tgtactacag gggatttatt tcttcaatga tacttttaca aaagaagaaa
     9361 agcaaaaaaa ctttgatttc tatgaaccaa tgactgtcca tgagtcttcc ctttcgccaa
     9421 gtatccattc gattttagca gcagaattag gaatggaaag caaagctgta gaaatgtacc
     9481 agcgaacagc tcgcttggat ttggataatt acaataacga cacagaagac ggcttgcaca
     9541 tcacttccat gactggtagc tggttgtcga ttgtccaagg atttgctcaa atgaaaactg
     9601 cccatgatca gttgagattc gcaccatttc tgccaaaaac ttggaaagct tattctttcc
     9661 atatcaatta tcgcggacgt ttattgttca tctcagtcac agatgaacaa gttactttcg
     9721 agttattaga aggttctcct ctagaaattt acgtctatga acagtcttat tctttagcgg
     9781 atcgtctaga aataacgcta agaaaggagg aagcaaatgt ttaaaggggt attgtttgat
     9841 ttagacggtg tcattaccga tactgctgaa ttccattatc gtgcttggcg aaagcttggt
     9901 caggaaatcg gcattacgat cgaccacaca ttcaacgaac agctaaaagg cgtcagccga
     9961 gaagactcac tctctttgct tttagcttat ggcggaaaag aacactcttt ttcaaaggaa
    10021 gaatttgcag aattagcgaa aagaaaaaac gattattatt tagagatgat ccagacgatc
    10081 gaaccaaaag atgtcttccc cggggtcgtg cctttactgg attctttaaa agaagaaaaa
    10141 atcaagattg ctcttgcttc tgcaagtaaa aacgggccat tcctacttga aaaaatgggt
    10201 cttacacctt actttgatgc tattgccgac ccggcagaag caacaaacgg aaaacctgcg
    10261 ccagatatct tcctccttgc agcaaaagct gtcggactaa aagcagaaga atgtatcggg
    10321 atcgaagacg cgcaagccgg tatccaagcc atcctctcaa gtggcgctca accaattggc
    10381 gtgggacgtg ctgaagacct tggagaggag atcccgcttg tccctgatac gacttttctt
    10441 acgatcgatt acttgaaaga aaagtggcat gaccatggat aatccagaga tctccgtcgt
    10501 accagttcca gagactgaaa taaagaaaat cacattgaag aacgactact tagaagtcgt
    10561 tcttctcaat tacggtgccc gcctgcacca aatcttcgca ccagacaaag aaggaaaatc
    10621 agaaaatgtt ttattgtctt acgatcgttt cgaagatgtt ctaacagaca aaagcttttt
    10681 tggagcaact gtcggacctg tagccggaag gatccgggat gcttcttggg gtgagcatag
    10741 attggagaaa aactgcggat cacaccatat ccacggtggc acgaaaggct ggtctttcca
    10801 attttgggaa gtagaagttt tcaaaactcc atcatcaata ggtgtggtct tttacttaaa
    10861 agatgaattt tctacctatc caggaccaat cactgctacg atcacttacc gtttaacaaa
    10921 aaacgaatta gagatgacaa cctctgctag tagccttgtt gaaaccatct gcaatccgac
    10981 caaccatgcc tacttcaacc tttcaggaaa tggaacaaga gacatctatg aacatcagtt
    11041 aacggtcttt ttagatggtg tgcttgaatt agatcaagaa aaactgccta ctggaaattg
    11101 gacaaaaaaa gaaaaactgc caatcgactt tagaaaatcc ccaacactgc aagagatcct
    11161 tgcatgttat ccagatggac tagatgatgt atttcttttg catcatccta ggctttccaa
    11221 aacttcctta caattattcg agaagcattc tggtcgccaa atgactatag caacttctaa
    11281 taaaagtatg gtattatttt ctacgactgg attcgaagct gatttctctg tcaatggaaa
    11341 acagatgcat tcaaactacg gattagcaat cgaaccgcaa gaaatccctg atatcgttca
    11401 tttccccaaa tttggatcga tcaacctcca tcctggtcaa gaacggatca gtcagacgat
    11461 ttatcggttc tctgcacaat aacaagagaa aggtgttttt atcatggcta ttaccgtaaa
    11521 agatgtggcg aaaaaagccg gtgttgccac ctcgaccgtc tctcgtgtca tcaacgatca
    11581 tcctagtatt tccgagacaa caaagaagaa agtccgaaaa attatggatg aactaggtta
    11641 tgttcctaac atgactgccc gcaatctagg aaaacgcatt tccagtgcca tcggcgtaat
    11701 cttaccccct cttgattcaa aagagcggat cggtaaccca ttttatcttg agatcatgga
    11761 agcaatcaac gaagaagcgc ggaattatga tatgactaca gcaatcgcta ctgctaaaaa
    11821 ttttgaattg ctgttagaga acgtccagcg tatgcattta caaaaacaag tagatggttt
    11881 tattctagtg tattcagata gtcaagatac tgtcattgat tatttatacc aaaaccacat
    11941 ccctttcact ttgatcggac agccttatca tcatgagaac gaaatcgttt atgtagataa
    12001 tgataaccag cttctaggta aacaagcaac agaatttttg atccagaatg gacataagaa
    12061 tatcctgttt gtgacaaaca cgactcatga aagtttgtat ttcgaacgat atttcggtta
    12121 tcaaaaagca atgatgatgg ctggtcttcc tgcgttatcc agtgtagtac tagagcatcc
    12181 ggaggattat ctgtcctttg agaaaacgtt gaaagaaaac gaagccacag cattggtggt
    12241 cattgacgat atcttcgccc ttcgcacgat gcagttggcg caaatgtatg gctatgtcat
    12301 tccagagaca ctctccgtca ttagcttcaa caactcgatc ttctcaacat taacacaccc
    12361 ttaccttaca agtatcgata tcgatatctc cgaattagga cgtgtcgcta tgcaaaagct
    12421 gcgcgaattg atccatgaag aattgagcag tggcgtacgt ttggtcatcc ctcatcgctt
    12481 gatcaaaaga gagacagtcg ttcctttaga aaacccatac taaatagctg actcactaaa
    12541 tacgctgacc atttatccta tggtcagctt tttatgtaaa agaagatact acttctaaca
    12601 cattgtttat ctgtaaaaaa gagcgtggga caaaagtaaa gaacactttt gtctcacgct
    12661 ctaaacacgt ataaacggcg gaagcagaag caactccttc ggaaataagc tgaaattcac
    12721 aaaaatttaa aaagcaattt tcttgagttt cttcttattt cttggagtta aacgcttctg
    12781 tcccagcctc ttttttcctt ccttcaattt tttacacgtc gacgattcaa taaaaaaccg
    12841 cctaatattg taaagacagg tgcaagaatc gagaaaaagg cgaatggcaa gtaatgcaac
    12901 gcgctaactt tcatcgttcc cataatgaat gtgccgctga ctccccacgg tacgagagaa
    12961 ttgacggtcg cacctgaatc cgctagtgtt cgtgtcaaat aggtttttga taagccaacg
    13021 tcatcaaaag aacgcttgaa tgtttctcct ggaagaatga tggacagata ctgttctcca
    13081 acgatcagat tgattccgat acagctcaag gcagtaaacc cgactaaacg agaaggtcgg
    13141 tccatctttt cttttaattc ttgaacgatg gtatcaacga tcagatattt gatcagtaac
    13201 ccacctaatg ccaatgcaag taaaatcaaa ctagcagaac ctaacatact catgatcccg
    13261 cctcgagaaa gcagttcatc tagctttgga tcaggtgtat ttgcgacata accattcatc
    13321 aagatgtctg ctgcttttcc gaccgtcaaa cttttctcat ggataaaaga aagaacgaga
    13381 gcagctgtcg atcccgctag taatgtaggg atagccggaa tttttttgat tgccccccca
    13441 aataaaataa ctagtggaat caatgtccaa ggagagatcc aaaagctcgc tttcatgttt
    13501 tggatcattt cattgatact agctagatcg gctgaatgac tgttatgtcc taatatcgta
    13561 taaaaaatca acgaaaggac gaatgctggg aaatccgtat aacgcatacc agcgatatgt
    13621 tcgaatagat taactcctcc aatacccgct gccaagttgg tcgtatctga taaaggcgag
    13681 cagttattcc ctaaaaaggc accggaaacg actgcgcctg tagtcattgc atcattgaat
    13741 ccaagtaaat gaccgatccc caaaaaagca attccaatcg tcgaaatcgt tgtaaatgaa
    13801 ctgccgacag tcactccgac aattgcacaa acaagaaatg ctgtcggtaa aaagaaacgc
    13861 acagaaacaa gtttaaatcc ataaaccata atcgtaggga tggttccaga taaaatccat
    13921 gaagcaacta agacaccaat caaaagaaaa atcaggattg gaatgatccc tggagtgatt
    13981 cctttgatga tcccgtcatg gatctcatcc catgtgaact ttttaaatct gccataaaac
    14041 aacaaacaca tgaaagctgc aaggatcggg atatgtggcg agagctggaa tccgatgatc
    14101 agtactccta ggagtgccaa caacacgatc aatacagcga tactttcttt taaagaaata
    14161 tttttattca tcgtcgatct cctatttcaa atgaaaaggc cctgccatga aaaaactatt
    14221 cacttggcag gacctcctac taaatcagcg acaaaacgtc ggctgaaaga cttatttttt
    14281 tatttttctt ctaaactttt ttctacctca atcccccaag aatctttctt gaagtgattg
    14341 atgatcaatg gaataacgaa catgaccact gtgatagctg ctactaataa tgcatcactc
    14401 aatgtcgctg tacctaatgt agcaaatacg acaacgatga tagagaagat cagcatgcag
    14461 ctgactaccc aagctaatcc gtttccgttt ttaccaattc ggtaaggacg ttcgaattct
    14521 ggatgtttct tacgcatttt aagcaatgaa acagcaatca aaatgtacac gatacaataa
    14581 ataacagttg tagcatttgt aagtgtcaag aatacagcac ttacgtcatc cattacacca
    14641 taaagcaaag caaataatga aataacgatt gattgtgtta gaacgacatt tctagatacg
    14701 ccgtatttgt tttctttatg gaatccgaat tttggtggaa ggaatccttc acgagctact
    14761 tggatgattg tttttgatgg tccagtaacc caagctgata attgtaaaag cacaccaata
    14821 aataccatga agctgaagat atttccgatc acttctggta agcctaggat ttgacagtca
    14881 atcaagatag gctgcgtgat atttgctaat tccatttttc catctggtac gacatttgct
    14941 acaagcatcg cattgatgac attcaataaa actaatccga tcaaagcgat aaatacgcct
    15001 tttgtataat tttttgttgc gtctttcaaa cgaggcatgt aaacagaact catttcgatc
    15061 ccaacaaaaa taaatgtgat accagctaag tatttcaatg aatcaatatg ttccaagtca
    15121 gggaaagctt ttgaccatga aaagtcacct agatagccgt ttgagactag tccgactttg
    15181 aaggctgcca aaacacctaa aacgaacatg atgactactg ggatatatac ccctagccat
    15241 acaccgatgt tcccgccgac ttttgccata tcgaatttca agttcaagat cgtgatgatc
    15301 cagtagataa ctaaaataca acctaaaacg aaccagtgat tattgccaag ttctacatta
    15361 ccaaatgtgt ttcctaacaa tggtcctaaa gtagaagcga ccattaccat acctgggaac
    15421 atttgcaccc ataatagcca tgcaacgaca aatccccatt tgctgcccag tgctgttttt
    15481 acccataact gaggaccgcc ttcttgagga agcatcgtgg aaagctcacc ggaaaccatt
    15541 gatatcggac cggcaaaaaa caaaactgca aaaattgaat aaaaaatcaa tgtccaaccg
    15601 actgcggcta acgtaggaat actccgaacc gttgcacaca gtgccattgt cagaccgata
    15661 aaggtcccaa gacccatttt acttacttca gatttactca ccgttgacac tctccttgat
    15721 tttttggata aaataacggt gtctttgaag tagtttcagc aataaacgaa acaattcagc
    15781 gaatatttga ggggattctt tgagtttatt gcttgaaggg tttcttcaaa gacaacctca
    15841 aaacaaaggg atcgaagagc ttaggtctcc ccttgctctt cagatccaaa catgcgtcag
    15901 aaacagttag tattcaagct gttttctaac atgattacct tgcttatttt gcttcgcttg
    15961 ccaataattg gtctgcgtag attttttcca atttttcttg aagagctgct ttgatttttt
    16021 cagcatattc ttcaaagtgt tcttctttgt tcatgtatgg tgtcattacg ctggcacgca
    16081 atacagttac ttttccggca cggttccatt cttcatctga gaatcctagt tggttcacaa
    16141 attgtaatgg gctgttgcca tagtctggga tagcaaagtc agtatgtgaa gttaagaact
    16201 cattaccgta aatgcttcct ttcacgtatg aagaataatc atatacgtca tggttcaatt
    16261 tattcattgc aactaagtca tcgttgcctt tttctttgaa tacatagtct accatattga
    16321 aatctggata agttaatgga tgaacttcga tttctttgtc tcctacttta aatgacaagt
    16381 cattcaagaa gttgtagaaa cgatgtgcac cttcgattga tgcacccatt aatttaccat
    16441 atcctgtgac attcaaaggt aagacatggt gtgctgccca aacgctggct gctgttgccc
    16501 ctgcttttga accttccaag atataagccc caagtaatgc tgggatatct gcgccttttt
    16561 caaatacata tgtcgcaaag taagagataa cgtttctcat gcggatatct ttgatgacga
    16621 taccaccggc agaatatgga acatagccca ttttatgtgg gtcaatcgtt acagattctg
    16681 cttcttcgat tgctttatat gcactgtgta cttcttctaa gatataatct ttgttttctg
    16741 taaagacatt gtatttgtaa tgaacatctt ttaaatcttc aaatggaatg aagttgttgt
    16801 cttcgtctaa gaaaatcgca cggccgtatc cgccataagc agcatctacg tgtaagtaga
    16861 agtaaatacc gtctttttct aatacgcgac gcaatgcaac gattttatcg ataccgtcga
    16921 ttgctccttc ttctgttgat cctacaacac cgactacacc taggattggt gttttttcag
    16981 ctgctagacc acgaacgatt ttttcaagtt cgttgatgtc catgcgatag ttgtggtcaa
    17041 ctggcactgg aatcacttgg tctaaaccga taccgatgat gtcggcagct tttagccatg
    17101 aatagtgttt agtttgtgga accaaccatt ttcctaattt ttctaaatgt tttccgctac
    17161 gtgctgagtg agctttgatt tcgtcgattt tttctgggac gctgtctaac agattcatga
    17221 tttcttctgt tgacaagttc attaattccc aatcgctttt tccagcaact agttcaggtg
    17281 tcacttcttt cattgcaagc ggcaatgatt tgatgttgcg ggcataccaa agtccttcta
    17341 agtttgctaa agaaccatca gcaacgatgt ggccccagcc atctttatag ctcattaatt
    17401 ttgcaaattc cattcctact tcttcttcca tttggcttgt tgcaggagaa gattcatagg
    17461 caacgttatt accattccat agcatagcaa aattgtacgc tagtaaagat ggcatcaatg
    17521 tttcagagtt catatgaccc cagtaacgtc cggcattatg ccaaggaacg gaatgggtac
    17581 gcatccgcgc tgaaatttct gatagaacat cctttgtttt attcacagtg tgttcaaaac
    17641 tagcactgct tttttcttct ggcgtaataa taggcatgtc ttgaggcatg tagttttgac
    17701 gccagcctaa atgttcatcc accaattcat tcaacaaagc tttatagatt tggccgttct
    17761 cagctttgtc cccaatgaat agggcattta ggtttagatc tttcgacaat gattcactca
    17821 tagtgaaacc ctcctaaaaa aatatttaaa ttttgtcgag ccctacatta cacctgttct
    17881 tctattctgt aaactaagtt ttggcctgat ttttaatcat attttaatat gtatcaatta
    17941 tccgaattgt tcacactttt gaaataatta aaaaaaacga gggaatttac aaatgtaaac
    18001 tccctcgcaa agattgtatt aacgacaacc agcgttctat tttaggatgg gtttcaaaat
    18061 tggaatgttg taatagtatt tccctttttc aaggtaaaaa cttactaaat tctaatcaat
    18121 tactttggcc aaaaagtaat ttttcttacc ttttcgaata accacaaact taccatcgaa
    18181 ttcatcggat ggatttatga caaaatttaa atcggtaacg cggtcaccgt taatacttat
    18241 agctccgttt gaaacatctt cacgtgcttg acgtttagat ggttcaattt ttgtggaaac
    18301 aagcagttcc acgatatttt caggtgtgct tgaaatttca acgttcggca ttttaccgaa
    18361 tccttgcgcg atttcttccg catttagttc tttaatattt ccagagaaca atgcttgcgt
    18421 tattttttgc gcttctttca agtcttcttc actatgcaca aatcttgtaa cttcttctgc
    18481 caaacggcgt tgtgcttcac gtttctcagg ttctgtctct acttttttcg ctaagtcttc
    18541 gatttcttct tgagacaaga aagtaaagaa tttcaagtat ttcactacat cgcgatcatc
    18601 ttgattcaac cagaattggt agaactcaaa tggtgaagtt ttcttcggat caagccaaac
    18661 ggcaccgcct gcagtttttc cgaatttcgt tccgtcagcc ttcagcatca atgggatcgt
    18721 taaaccgaac gcttctgctt ctggtccttc taattttcgg attaggtcta atccagctgt
    18781 gatatttccc cattggtctg ctccaccgat ttgcaattgc acatcgtatg ttttatgcag
    18841 atgcaaaaag tcgatagatt gcaggatttg gtaagtaaac tctgtaaatg agattcctac
    18901 ttccaaacgg ctggcaacga tatcttttgc taacatcgtg ttgatgttga agtttttacc
    18961 gtagtctctc aaaaattcaa gtaaagagat ttttgacaac cagtcgtagt tgttcacaaa
    19021 tgtgatattt gcatctttac caaacaattt tctcatttga ttggaaagtg cgtccacgtt
    19081 atgttgcact tgttccatcg tttgcaacac acgttctgtt ttgcggccgc ttgggtcgcc
    19141 gatcgttcct gttccgccgc caatcagaat atatggatga tggcctgcca attggaatct
    19201 tttcatcatc ataaatggaa tcaaatgtcc gatatgcatc gaatcaccag ttggatcaac
    19261 gccgcaataa agtgaaatgc tcttttcttc aacgagctcg cgaagtcttt cttcgtttgt
    19321 ttgctgatta atggcgtcgc gccaagttaa ttcatcaata atattcattt tactgacctc
    19381 cgtgtagttt ttttatgaaa gcaggaacct gctagcatat gtaattaatt atttcttcag
    19441 gataggatag gaaataaaaa aagtcccttg gcatataagc caagggacga aatctcgcgg
    19501 taccacccaa tttgtatgtc tagatatcca acatacctct cgaacattta acgctgtcgc
    19561 gtggcaatca ctgccaaaga ctcctgaaat gtaattcgct ttaaagcatg tactggtttg
    19621 cagcgaccac cagctttctg aaacattggc tttaacaact acttcgtttc gatcttcgtc
    19681 ttgttattat taatatgtgt ctatcataga gcgcaacttt ttctttgtca acagaaaaag
    19741 gatatttttt ttaaattcct cattttttct tttttgaatc cgctctaatt ccgaacattt
    19801 tattttcaat tattgttaaa agaaataact ttttctgtat tttttctgca atttatcgag
    19861 aagtcacttt actttgcctc ttattctttc ttcgttacaa taaggaatgg gacaagtaaa
    19921 aaaatgaaaa agcaagggac gaggtatatg gaaaaaaagc aaaaaattat tttaggagcc
    19981 acttctgctt taactatcgt tattttaatt ctaagtttgt tttttgtcat ccgatcatct
    20041 acaaaaaaag aaccaatcat tcctgaaaaa aaaataacag aaaaagcaaa agaaccgaca
    20101 gatgataagc aacaactagt tactcaagca aaaaatctgg ctcaaagcta cgattatgac
    20161 caagcgattg cgttattgga aaaagatagt aagaacggat ctactgaaac acaacagctt
    20221 cttcaaacac taaaaaagga gaaagaacaa ctgatcgact ggtctgatcc tactcagatc
    20281 tctcatgtct ttttccacag cttgatcgtg gacccgcaaa aagccttcca ctctcaacag
    20341 gcacaaggat acaaagatta tatggtgact gttgaagaat tcaaccgttc gatcgaacaa
    20401 ctttatcaaa atgattttgt tttagttaat ttgaatgatt tgatccaaaa agacgaacaa
    20461 ggaaatcttt cctttaacgg gcttgcgcta cctgaaggaa aaaaaccatt ggttctttca
    20521 caagatgatg tcagctatta tgaatatatg gataattccg gttttcctag ccgattggtc
    20581 gtatcaaata acaatgaaat caaaaatgtc tataaagaga aagataaaga agaaatcggt
    20641 gactttgaca tggttcctct gatcgatgcc tttgtcaaga aacatcctga tttttcttat
    20701 cgaggcgcaa aaggtacctt ggccctgaca ggttacaacg gagttttagg ttatcgaaca
    20761 tcaaaatctc aatatggaga caatgaaaaa acaaaaaaag agattaaaga agctaaaaaa
    20821 gtggcggaac agttgaagaa agacggctgg agctttgctt cccatacatg gggtcacttg
    20881 aatatgactc aagcttcact agaagatgta aaaaaagaca atgaattgtg gcagcaagaa
    20941 gttgcaccta tcctaggaaa aacaaatatt ctgatttatc catttggtgc agacatcagc
    21001 gactggcagc cttattcagc agaaaatcaa aaatttgctt acttgaagag tcaaggtttc
    21061 gatattttct gtaatgtcga tgcttctaca ccagcttggg gacaattagg tacggatttc
    21121 tatcgaaatg ctcggatcaa tatcgatggc attcgttttc aatctgattt agacggaaaa
    21181 aatcctattc ttgatccatt catcaatgtc catgatgtct atgacaaaga agcacgcgaa
    21241 tctgcttgat tttacgtcat ttatgaacca aaaaagccga actgacattt tatcgtcagt
    21301 ttcggctttt ttatgcttta tttcttcttc ggtgcgattc ccttgatcag gacttctgtc
    21361 cattctgctg tatatggaag cagttttgcg tatatctccg gcttagaaat tccttctacc
    21421 attgcttgaa aatagaggat caagaactca tccgaatatt tcaaatccac ttttccttct
    21481 ttccgccctc gatggaaaag atcaagcatg atactgaaac tttcttgcgt atacgcatcc
    21541 atcgcgtcag aaaattcttt tttttctttt tttgtatagt atgtcatcaa atcgatataa
    21601 aattgtttgt tcactcgatt gagataacgg attttgtttt cactcatggc aatcagtgtt
    21661 tcttcaaatg gaagattttc cgcaatcaac gctttcgctt cttgccctaa atcattcata
    21721 aatgcaaaaa atacttcata aatcagccct tccttgctgc caaaatattt aaaaatcgtc
    21781 gctttactta cctgagcgtt ctcagctacc ttatccattg tcactccagc ggttcctatg
    21841 tctgtgttca tcaagtcaaa agctgcttgt aaaatctctt tctttttctc ttgtgctctc
    21901 ttttcaaaac cgttcatttg tagagcctcc cgtaatttta aacttttata atatatatag
    21961 ttcaaattta ttctgaggtt tattataact atctattgag aaagtatcaa gctagcaatt
    22021 ccttgtttat gaattcattg acagaaattt ataaaaagaa tattatgtac taaaataata
    22081 aaaaaagttt tataaaaagg tataaaacct ttttattaaa ctatttaatc ttgtttagta
    22141 tatattttta gaaaaaggag tggggttttt atggcagaaa tcgtcaaagt cacacatttg
    22201 aaaaagagct ttggtcgttt cgaagcttta aaagatgtct ctttcaccgt acattcaggt
    22261 gaagtcgttg gatttatcgg accaaatggt tctggaaaat ccacgacgat tcggacactt
    22321 ttagggatta taaaaaaaga cggcggaacg gcaacgattt ttgggcaaga tgtatgggaa
    22381 gatagtttat ctatccatga acgtctttct tatgttccag gcgatatctc tttgtgggga
    22441 aatttgactg gaggagcgat catcgatctc ttcatgaagc ttcatggaaa agggagtgct
    22501 caaaaaaaag atgaactgat tcaacgtttc tctcttgatc ctaagaaaaa agcaaaaaac
    22561 tattcaaaag gaaatcgcca aaaagtgggc ttgattgctg ctttggctgt tgattcggat
    22621 ctctatttat ttgatgagcc tacttctggt ttagatcctt tgatggaaac gattttccaa
    22681 gaagaagttg aaaaattgaa agaacagggc aaatccatcc tactttcttc tcatattctc
    22741 agtgaagtcg agcgactagc ggataaagtc gtgatcatac gcgaaggaac actcgatgaa
    22801 gaaggaacac tcgatgaatt gcgtcatttg actcgttcga ccatcactat agaaacagaa
    22861 gcagagaata gtcacttagc acaattgaac ggtgtccatg actttgtgca aaaaggtcac
    22921 ctcgctcaat ttgctgctga taacgaagct ttcgatgtga ttttagctga agcgtccaaa
    22981 ttaggtgtga aaaaatttga atctattcca ccgactcttg aggatttatt catgagacat
    23041 tatgaaggat gaggaggaag cgaaatgact gataaatttt cacgctggtc cacccttttt
    23101 tctcaaaacc ttagccgtga ctggaaaaag atcctgatat ggctagtcgg tgtcactgct
    23161 ttttctggtg gttttgtccc tgcgtttgaa gaattgactt ccggacaagg attagctgga
    23221 ttatacgaaa ccttgcaaaa cccggcaatg attgcaatgg tcggaccaac tccagcttcg
    23281 aatgctgctg agtatacttt aggagcgatg tatgcaaatg aaatgttgct tttttgcagt
    23341 ttgtttgctg ctgtgatctc tgttttacac gtggtgagcc aaacgagaaa aaaagaagaa
    23401 cttggtctga ttgaacttat tcgttccttt cgagtcggac gtcaagcaaa ctctttagct
    23461 gtcatcgctg aaagtattct gatcaatttg cttttagcaa tatcgatcag ctgtacgatg
    23521 atttttttcc aagcggattc gatcactgca gaaggtgctt ggctttttgg gctatctgta
    23581 ggtagtatgg ggattttggg gagtgccgtt gcactgtttg ctgcacaact gatgccaagt
    23641 tcagccagtt cgaatggtct gtctttagcg tttttaggtg gactttacct tctacgagca
    23701 ataacagatg tagctgattc ctccctttcg atgtggaatc ctttaggctg gtcttatttg
    23761 acctatccat ttacaactaa caactggatt cctttgcttt atttgttttt gttcagtcta
    23821 atcttgataa tcagttcatt tgttttagag gaacatcgag atttgaacac cggctatatc
    23881 cccgaacgtg aaggaaaacc gtatgctaaa cgttctcttt tatccttgcg tggtttatta
    23941 ttctatctaa ataaagggat gacaatcagt tggcttatca cttttggttt gatgggggcg
    24001 gcatacggct ctatctacgg gaatatgcaa cagtttttag aaagtaacga cttaatgaaa
    24061 cagatgttta tccaatctgg cgtctcaatt gaaaaaaact ttacaagcac gatcatggta
    24121 gtcttgattt gtctagctgc tattttacca attgctagca tcaatcgttt atttacagaa
    24181 gaaacccacg gacgtatgaa tcagctgttc gcaacaaaaa tttctaggaa ccaactttat
    24241 tggaccaata tgttactagc tattctattt gctatattgg caatcctgat tagttctggt
    24301 gcactagggc tttctgcttt ggcttcgatg aaggatgcga aagacatgga tctattgatg
    24361 tttcttaaag ccgggttcaa tttcctgcct gccgttttag tgtttatcgg aattagtggc
    24421 tttctattcg gatggcttcc tcgaatcggt aaacttgctt atatctactt agcttactcc
    24481 tttgcactca attactttgg cggaattttg gatttaccag aatggttttc caaaacagct
    24541 gtccctagct gggttccacg gatgccaaac gattcttttg atctttccgt gttccttacg
    24601 ttatcatgta tcagccttgc actgttcgtc cttggttcta ttggttatag gcgaagagac
    24661 ttactagaag gataataaaa aagcatgtac aatttcatgc tagcctccaa atctgggtca
    24721 aaaatccaac atttggaggt cattttttta tttacaagtc ttctaactgc tctttatcaa
    24781 tgatccagac ttctcgattc tttcttttta cataaccatt ctcctctaat aatttaaatt
    24841 ttctagataa agtttcgggg gtcgtaccga taaatgcagc aagttctttc attttcattg
    24901 gcaggaaaaa gtgatccttc cctgtcgctt tagctaagtc aagcagatag acagcgactc
    24961 gttcttctac tttttccatc atcaaaaaag ttgtctgctg ctgtactttt gttaatttct
    25021 caacgttgat tgctaaaagt ttcagactaa gggcgggata atgcagtaag agttccgaga
    25081 aatctttttg tctcaatatg caaatttccg ttttttctag tgcttcgcca aataaagact
    25141 catttggaac gcctagcaac tggctctctc cctcatatcc gcccggctct attactcgta
    25201 aaagctgttc ctttccgcta gcagataatt gatacacttt catactgccg gaggccacga
    25261 tcactaattg aggttctcca ttcggcgcaa gaatctgttc tcctttttcc acatagtgat
    25321 gtttgacaag tgcgtgtact ttttcttgat cttgctgtac taagtgattg aacaaaggaa
    25381 caaatgaaac acataaatga tcatgactca taatctctcc tccttctaaa cagctatttc
    25441 taataaagaa acataacttc ccttattcca actgataatt ttcttttatc atagcataaa
    25501 cgtcaagcga accagatacc agcaaataga gtggatttct ttttgtccct tgctttattt
    25561 tccttctttt gagaccttaa gtaattgatt cctatcaatt ttttaaatat ttactttttg
    25621 aataatcaaa aacaaacctt cactcgcaag ccgagagtaa ggtttgtttt tcgcattgct
    25681 tatttcgtgt tgtctattga aatttttccg cacctagctt ttgatatagt ggtgctagtt
    25741 cttgtaaggt ttcagcggtt atttgctcaa cgtgttcgat ttcttgcgga tagaagaata
    25801 gtttacccat ttgaaaggtt cggtctctca tcgctttttt caactcttct tgtttctttg
    25861 tatcggacaa atagtcagac aaagctaagt ggtcgtcaag ttcatgttct ggttgcggcc
    25921 atatgtaata atccttgata tcgactgttt tgctccactc tttcagtatc gcaagcaact
    25981 ggttatacgc tgcaatgcta ccatatcgtt cttcgctttt ataatgttgg tacatcaaat
    26041 agatttggta ttgtttttga ttcaacaaaa ctgctagaca ggcattctcg tcttgacgag
    26101 attcgcttcg gtacgcgctc caaaaatgac tgcgtaaatt ccagccattc gtccagcttt
    26161 cgattttagg tttgctcatg ccgtacatat caggcaaaag tgcagctgtc tggagctgaa
    26221 tctctttcca tttttgccag tgttctttat attctttctt gatttgttca atcttatctg
    26281 ttggatggct ctctttgagc tgtttgaatg cgtagattgg tcgatcaaac aactgataat
    26341 ctgattttcc aagttccatt gtttcgcctc tttccgttat cttttgtatt ctacctgaat
    26401 tttgcgatat ggacaaatag aggttgtgaa aaagctgttc tgcatcaagt cataagaaca
    26461 gccaaacaaa aaactgcgat gagcatcacc gcagttttat tgaacttaag gttgattgat
    26521 cacatctaaa agcgttgaaa ataaaatggc actagaagca gctcctgggt cgatatgtcc
    26581 gattgcacgc tctcctagat aggaagctcg tcctttttta gctaccaaga ctttggtgtg
    26641 ttcttttgcc tggtcaatga tttcttttgt taaattccct gccttcaatg cctctgtgac
    26701 tggcagccaa acatctaaca tcgttttatc gtcagcttct gcctttcctc ggctttgaat
    26761 acccgctgtt ccctgctcga tcacttcgcc taatttttct tgagaatcaa tagaatcgac
    26821 ctcttttgtc gctttcatca tgttcatgaa tgcagatccg tataacggac cagaagcccc
    26881 accgactttc gatatcatcg ccatagagaa tactttgaat gtatcgctga ttgtttcagg
    26941 atgatcggct tgaaaagctt cttcataagc tgaaacccct cttcccatgt tatttccatg
    27001 gtctccatcg ccaatcgggg tatctagatc actgagatat tgtttgttct gattgattgc
    27061 ctctgcaaat tgcgtgagcc attcttggat ttcttgaacg gttagtttca tattatcacc
    27121 cttttatctt gagatttttt accatgaaat cgtctgtaca ttgctttcta aagaatcttt
    27181 ccattgtgtc tccgtcaagt cgatcagtgt tagagaaaga ccttgcatat ctagtgaggt
    27241 cataaaatta ccgacttttc ggaaaactac attgacgcct ctttcttcca acagagctaa
    27301 aacatcattc atgaacacga actgttccat caaaggcgtc ccgcccattc cgttgactag
    27361 tacacctgct tcttttggta aattcgagta atcatctaaa atcttagtaa caagttcctt
    27421 tgccagtact ttagaaggct gcatcttttc tcgacgatag cctggctcac cgtggatacc
    27481 aacaccatac tcgatctcat cttctcctag ttcgaatcct ggtttcccta cttctggtac
    27541 agttgctgct cgaagcgcta gaccgatcgt cttagttgcc tgtacgacct tttctcccag
    27601 ttctttcaac tcagctaggg aagcaccgtt tcttgctgca tcgcccacga ttttgtgtac
    27661 aaggacagta cctgcaactc cgcgttttcc agcagtatac gtactatttt caacggcgat
    27721 atcatcatct acgatcacac tttctacttc aatatcgtcc atagcagcta attcctttgc
    27781 catatcgaag ttcaatgcat cccctgtata attcttaacg atcagcaaaa ctccttttcc
    27841 actatcggct tccttaatag ccgtctgaat ctgatctgga gttggagaag taaacacatc
    27901 accaagcacc gctgcactta acatcccttc ccctacaaac ccggcatggg atggttcatg
    27961 tccgcttcct ccaccagaaa ccaaaccaac ttgttccgct ttctgattct tggctacaac
    28021 acgggaagat tctacacgat gaacaagttc tggatagctt tttactaatc cttccagcat
    28081 ttcttcgaca atattgcttg gatcattcat gatttttttc aatttattcc catcctttct
    28141 ttccggcatt ttgtgcttct gttaagattt gctgagtatc atctgttata ccggcagtga
    28201 tggctgctgc aaaagctcct tccactaaag gcgcatccac cagatgataa cgttgctgct
    28261 gctcttcttc caacatgtct tttgccagtt ccgagttcaa taccgcgcta ccgatatccg
    28321 caaaaacaaa ataactagct gcatcgcttg attcattgac tgcttctacg attttcatcg
    28381 ggtctgatcc gatctcgcca tttattccgc cgagagaaaa aatttgcacc tcttcggatt
    28441 tttgcatctg ctcaatcatt tctttaatac catctgttac tttttgacta tgagaaataa
    28501 gcaaaatact ttttttcaca agaatcttcc tttcttttac atagttgcta acgattggtt
    28561 tcgctttcat taatgaacaa ttcaagtata taataaattt ctaattttgg gaaaatatgt
    28621 gtagtgcttc ttgaagtttg ttatacttta attaaaagag aggagtgatc gaatgattac
    28681 aacaacaacg aacagtgtag agggaaaagc cgtagtagct tataaaggga tcgtttttgg
    28741 tgaggtcatt actgggatcc atgcatttaa agacttggag gcggggttgc gaaatatctt
    28801 tggcggccgc tcaaaaagct atgaaaacga actaatgggt gcaagagaag aagcacttgc
    28861 cgaaatggcc gaacgtgcaa aagcattagg agctgatgcg atcattggcg taaaaatgga
    28921 ctatgaagta ttaggcagcg acaatggcat gctgatggtc acatgtagtg gaacagctgt
    28981 ctccacgaga taagtagggg acatctgttt taataactaa agagagatgg gacatgtagt
    29041 ggcactcaag aattagattt ttcagtctaa cttcgaggta ctctaccttc ccatcctctc
    29101 ttttttattt ctttttgcgg atgattccgt aagctgctgc gactaacaca cccgcccctg
    29161 ctaaggaata actgacagaa gctgtttttt catttgtttt tggcaaagtc tgacttgttg
    29221 atttggatgg ctctgtcgtt tgctgaactg tttctgtcgt gcttccagag gtcactgatt
    29281 tttctcctgc cgaatgcaaa atatctttta ttcgttggaa actagctgct aagtcagctt
    29341 tttttgcttc aaaatcatta gcaactgttt gtgcttcgct gatctttccg tggattgctt
    29401 gactggccgc tgtgatatcc gcttgagttt gacttagctt tccagaagct gtttgagcaa
    29461 cctgatttaa ttgctgattg aattcttttg cttgttggat cttcccgtca agatctgttt
    29521 ggacagcttc cgcttttgct ttcgcttcgg caagcttttt ctcaagttgt gcacggtcat
    29581 ttcccacttt atttttaaga tcagctaaat cttgatcgat tgctgcttgg tcttctttca
    29641 atttgttttt tgcagcttct aatgcttctt cgagttcttt tcttttattt tccaagtctt
    29701 gcaaatgatc gatttggtca gaagaggtag aactatttat tgatgtatct tctgaagatg
    29761 aaatcgtttc tcctgaatta tctgtcggta cattggaagt catttctgtt tccgaggcac
    29821 cgttttcagc accagaagag cttatggttg ttgaaggttc tgtttgagca ggttcagaag
    29881 tttccgcaaa agctgtctgt cccaataaga aaatactgga aacagcaatt ggccagatta
    29941 acggcttcat tctttttttc atcacttttc ccatccttac tactttttaa ctaatttctt
    30001 attataagca aaaatgtttg gaagaaccat aaacacaaca ggctgtttgt ctaaaaaacg
    30061 gtgcatcttt tatttttttg tacgttccgg gcttttccat gtcccgcttc cataacctaa
    30121 aatcgttttg actgctggaa tgaaagtcgt cacgatcgtt atcgcgttca ctatccagaa
    30181 caaaagcata tatagtggtg cgaaaagaag gtatttcaat tttcttcgtc gatcatctac
    30241 aatcagcgaa gctagcaact ggaaaaatcc tgcgaccatt tcaaagcaga tgaacacaaa
    30301 actgaatgta aaagtaatat aaatctgttc gtagtttcct tgatatacat aatgcccgat
    30361 taatccagca aaaagaacta cggaaataca aaagaatatt gaccagacaa tactcaatgt
    30421 ttgatcaatg aagatgatcg ttcttccaat atgtttaaaa ggatggagca tcactttttt
    30481 gaaattcgtc agccagactt ctgttccacc ttttgcccaa cgttttctgt gtcgatatag
    30541 catcttcaac gtcacaggta cttccatgaa aaagataatc tcaggagcaa aaacagataa
    30601 ccagtcattt aactgatgat cccaagcaat actgatgtct tctgtcgctc gatcttgtct
    30661 gaatccgccc acatcgatca aggcttcttt tcgatacatc gtatttgctc cactgtacgc
    30721 ataaagacca ccgaaaacag ctgactgtgt tcgtttaatg atgcccacga tacttgagaa
    30781 ttcaaccgtt tgagattttg caatcaattt ggtacgattt tggacatcca tatttgcagt
    30841 gaccgcagca atatgtcttg caccagggcg aatgaaataa ttcacatacc taatcagtgc
    30901 atctggctcc ggtaccgtgt ctgcatcatt gcttaaaatc aatttccctt ttgcaaaggc
    30961 aagtccgata ttaaacgcgt gggcttttcc tttatttttt tcgatccgaa ccacacgaag
    31021 attagcgtat tttttcatca gtctagctaa tatttctggt gtttggtccg ttgaaccatc
    31081 gtctgtcact aatacctcat agttgtgata attcaatttc gtcatcaaat actcgattgt
    31141 atattcgatc acaatttctt cattatgggc tggcaccatg atcgtgatga acggttcgac
    31201 actcaaggga acatctaccc attctttttg cttatgtttg tataaaaaca cataacacca
    31261 gacaccaata aaccatacaa atccgcctaa aatcggataa gaaacgagaa aaaaatacat
    31321 aatagacaaa caagtatcta agaataaatt acctgtatgg gtgattgaga tcatttcagt
    31381 tccaccccat tttctttata caaatctgca atgaatgtcg tatctagatt tttctcttcg
    31441 gagacagaat aaaaacggac cgtttctctc tcttcttttg ttccaaaacg ttccgtatac
    31501 acttcttcaa gtaattcttg tctttttttc aatttttcat cttcatattc aaaagattta
    31561 tttacttttt tgtatagatt tcgattgttc cacctagtca atacaaatga ggtaatcagg
    31621 aaactgaaaa aaatcaatac aaaaaagccg attaagaaat acagcatttt cttttctgat
    31681 aaaaaagcgt acaaggaaaa atccttggct ctttctggtc tcgttaatgg gaaaaagacc
    31741 catataaaag gaagaacaac gatcatccat cctaaaagag ccactaacgt ttgccaaatc
    31801 tttaaccagt aattaccttt tgaaaaatac ttatctttca ccaaaattga tgcaacttct
    31861 tctcttgtta cttttttcat atcctcacct cacgtttttt tgttacgacg taggagttta
    31921 ccatacattt catttctttt cacataaagt gttcctaaat ttttcctaag aaaaagaaca
    31981 aaacatttct catcagcaaa tgaaaaatat tttgttcttt ttatcagttg ttctttttat
    32041 tttttatctc ttaattatgt tgctctttcc aatacgacaa tgcttttcat tcctctagat
    32101 atcgccgtgt acatttgtct gcgtcttctt tgctcatctc catcgttttc aaatggatat
    32161 aagattacat gatcaaattc cagtcctttt gctagactca aaggaatgat ttggcagttt
    32221 tctaatccat atgaattttt tttaatcgat tcttgtagcg tttgggtttc ctcttctgtt
    32281 tgggtgataa ctgccgcttt ttctcctttt agagaaacca aaatctcagc tagtattttc
    32341 aacaattctt cagaggatcg aaaagagcgg tattcgggtt cttttccttg cggtcggatc
    32401 gatacgatgt cgatcttctc ttgatccaca gcataggttt tgaaaagctc tgtgattgct
    32461 ccgctagaac ggtaactatt tctaagatca taccgatgga tcggcagatg gtagtcgtcg
    32521 aaacaacgca taatatcagc aaaagtagtg gaagaattaa agattgcctg attctcgtca
    32581 cctactaaag taaagtgagt ttttggaaat aattcgatca atagacctaa ttgtgccagg
    32641 gtataatctt gtacttcatc aatcaataga tagcgaaatg ctggtacatt taccttttca
    32701 acaagtaaat gacgaatagc taatataatg actgcttcat caacagtacg tgtgggttgc
    32761 caagcataag ctgagccata tcgtttttca taaattcttt caaacaactg atgctcatcc
    32821 acccatgcca tttcttcgat ttgtctggtt atttttcgat attttttttg taaaagtttc
    32881 cttgcataag ctacaatact ttgttccgaa tcatcagaaa tcaatttacc gaataattcc
    32941 aactgcctat cttctgtcaa cgagagtacc tgattctgca cagcaggact ttttgcttgt
    33001 ttcaaaagat gattcttcca ttcgctcatc agtgcctttt tcgttgcttg aagcctatcg
    33061 atcaatttag gatgagcagg aactttctca tatatttttt cgatcgtttt tttagaaagg
    33121 actgtttttc catttttagt aagtccacgg ataaaattcg gatgatctaa aaacaaagga
    33181 tctgattgtt ttagattatc gataaacttt ttgctacgca aacgttctgt ctgctccgat
    33241 acattttctc ccgtgatacg tttgaaataa gcctcttcac cttctatttc ctctgctgaa
    33301 agctgtgaaa ccagctgtat gatggttaga ttcagtggat ttctttcacc taatgatggt
    33361 aggacattcg aaatatagtc gataaaccga tgattaggtg atagaatcag caagtcatcg
    33421 gatgtcatcg tatctcgata taaatagagc agatacgcaa tacgctgcat gatagcagag
    33481 gttttaccac ttcctgctac gccatttacc aagatatttt gacttttcac atcccgaatg
    33541 attgtgtttt gttccgattg gatcgtagca gtgatagcct tcatttcatt ggtctcatct
    33601 tggcctaaga cttctaataa tacgtcatct tgaatagcta cagacgtatc aaaaaacttg
    33661 accaaacgat ctctttccaa aagaaattgt cgtctttctt taataaccgt atcgatttta
    33721 ttttgacgaa ccgtataaga agatggacca atcatgttat tgtaaaaaag ttcggcaatt
    33781 ggtgaacgcc aatcataaac aagattttca tctgcatcat ctgtgaaatt tgctgtaccg
    33841 atataaaagg attcttttgg ctcattatcc agaaagtcta caacgacttt tccaaagtag
    33901 ggacttttca ataaacgttc gatctttgct agctgatttt ctgcgttttt ctgtttgata
    33961 ttcatctgat cgatttctct atttttcatt tcaatcatcg aaaaagtatc caggttatct
    34021 aagtaactgt caaaattcaa tcgaagctcg ccagccattt ctttcaacga ctggtttcct
    34081 tctatttttg ccgtatccat cgattcccgc agttcgtttt ttttatcaat tagcttttga
    34141 tacacgttct tcagatatgc ttcttctaac tgtttttgtg tgttgaccat acacattctc
    34201 ctcttttttt aaagtggtgg aaccgattag tttacctctt ttaagagtcg aatgcaagct
    34261 ttttaacgag tatcatttgt taggatatat cagttcttca ggtcggacag tttgatataa
    34321 agaaaatgca ccatcaaggt tttttacgtg gtagccagac tgcttcaaga ttctttccgc
    34381 tagatagcta cgtaatccgc tatgacagct gacgatatat tcttgttggc tgtctaattc
    34441 atttaatcgg tcccgaagtt cattcaaagg gatatgtttg gcttccttga atttgccctt
    34501 ttccaattct tctgctgttc gcacatctaa taaaattttt ccttttgcta gttctgtcgg
    34561 caattcatgc cattgtacgg tttcactaag cccttctgca atgttcattg ctgcatatcc
    34621 tagcatgttt actggatctt tggctgagcc aaatggcggt gcatacgtca attctaattc
    34681 cggtaaatcg aagatcgtca aatgcccttt gattgctgtt gctaaaatat cgatccgttt
    34741 atccacacct tttgccccaa caccttgtgc accataaatc tcgccagtct ctggatggaa
    34801 gatcaacttc agtaaaatat ctgttgcttc tggataatag cttgcatgat cttttcctga
    34861 tacatgaatg acttcaaaag acagttcgtt ttctttggct gttctttcac ttaaaccggt
    34921 ggatgcggca gtcattccaa acgttcgtat aattgctgtt ccaataccgc cttgatttct
    34981 tcgagaaatt ccggaaatcg tgtccgcaac ttgtctacct tgacgattgg ctggtgaagc
    35041 aagagaaata agtgcatctt ttccagtgat ttgctgtttt acaacgatag catcccccac
    35101 cgcataaata tcaggaatat tcgtttgata acgttcgtct actaggatac ccccgcgtat
    35161 gcctaattcg attccagcgg tcactgctag ctggttatca ggacgaacac caatggataa
    35221 aataactaaa tcagcaggga tggtacgccc atcatccagt cgcaatcgtt tcccgtggtc
    35281 ttcaaatcca gcgacagcat gagaagtaat cacttctact ccttgatgag acaattcttt
    35341 ttctataaaa gctgccattt cttcatccag tgttggaaga atatgtggtg cttgttcgat
    35401 caccatgact tctaaacctc ttctttttag gttttctgcc atttccaatc cgatgaatcc
    35461 tgcaccaacg atcacggcac gctttggctg tttttctaat gcatgcatca ctttatcgat
    35521 atctggtaca tttctaatag aaaaaacatt gtcggcttct gttatccctg gaatcgatgg
    35581 aacaactggt tttgcaccag gagataaaac caatgcatcg tactgttcaa tgtgtttttg
    35641 accctcatgt acgacttcta ccgttttgtt ttcgggaaag atggctgtca cttcatgatt
    35701 tggacgcaca tctaaacaaa aacgagcgga taatgattct ggcgtttgga caagaagatt
    35761 ttcccgttca ctgatttctc ccgaaagata ataaggcaag ccgcaatttg caaaagaaac
    35821 atacggtcct ttttcaaaaa caacgatttc tgcatcttcc attaatcggc gaagccgtgt
    35881 agcagcagac attccacctg ctacacctcc gacaataacg attttcatct tatttccctc
    35941 ctactgttcg atcaaaccat tgattcatgc caccacgtac attcactaca tcataaccag
    36001 atttctttaa ttctttagct gcttgtttac tcctcatccc tgattgacaa atcacataga
    36061 cggtcttttc tttgtctcca tcatatctgt ttatactttg taatggaaca tttttagctc
    36121 cttttatatg cccgccccgg tattctgcag gtgttctaac atcaagtaac gctggatttt
    36181 ccaaaagctt ttcctttaat tctgggactg ttatcgattt tattgattga aatagcaaat
    36241 tcattttctg cacctcattt acatttttat aaatacctca tggggtatat taaacaggag
    36301 ctttattaat atgtcaacca gaatgactct tcattttttt cactactttt tatatttgca
    36361 taaaataccg ataaggggta ttatctaatt ataaaaatag aggagaatgt gttatggaga
    36421 ttgataataa aaatatcctg aaccgtttga aacgtacaga gggacaaatt cgtggtatcc
    36481 aaaaaatgat cgaagacgaa aaagaatgta tggatgtcat cactcaatta agcgctgttc
    36541 gttctagtat tgatcgcgta atgggcatga tcgttgcaga taatctgaaa aattgctttg
    36601 agaaacctga tagtaatcca gaagaacaag cggctaaact agaacaagcg atcaagatga
    36661 ttattaaaaa gtaaaggaat gatttcaatg aaagtcgttt tccatattga tgaattagaa
    36721 aaatggtcag aaacagggaa aaacgtaaaa aacctgataa aagcttctcc agaaacgacg
    36781 attgtcgtat ctgtaaatgg catcgcgatt accggttatc tagactctgc caatgcagaa
    36841 ttcttagatt tgcaaggagt cgtttttcac gcttgcgcca atgctatgcg tgctaatcat
    36901 atttcagaaa gttctttacc tgaacaagta atcgttgtac cagcaggtgt ccttgattta
    36961 gttgaactgc aatcccaagg ttatgcttat atcaagcctt agtcagtaga ggttgcgaca
    37021 gaagtgatca gcttcaagaa atttcggctt atttttcgaa gaagctactt ctggaaaacc
    37081 gtgaatcaaa ggttgtgaaa acgctgtcct gcatcaagta ataagaacag ccaaacaaaa
    37141 atagcttctc atatttttcg tttggttggg cttattaccg cagatgcaag cttttgaaca
    37201 ccgtttaagg aagaggttgt gaaatcagcg gtttgaccta agtattagag aaaaaaacgg
    37261 gaaaatagct cctcatattt tgccgttttt tgagctaata ccgaaggtca gctgattgaa
    37321 caccgtttaa ggaagagatt gtgaaaaagt cgcttagttt cgagtattaa gaaaaatttt
    37381 gtgaaaatgg atttctacat ttttgcataa atttggctta ataccgagaa actggctttt
    37441 gaataccgtt taaggaagag gttgtaaaat cagcggtttg acctgagtat tagagaaaca
    37501 aacggaaaaa tagctgctca tattttgccg ttttttgagc taataccgaa ggtcagctga
    37561 ttgaacaccg tttaccaaga ggttgtgagc ctaccattta actccgaatc acaagaaata
    37621 atttcaaaaa caacttctcc tgtttttggt tcggttggct tattatcgag aagtagctac
    37681 tgggagtcta tttatcaaaa aaaggaatgc gacataggtc acaccccttt tttattttct
    37741 ttgaaacatc tttcggtact cgacagcata caagacaaaa gattgtaaaa gaccgtaagt
    37801 gatgacggca aaaatgactc tcacccacag cgttggtaat tctgggaaaa gcagcaaaag
    37861 gatcatgcca acatagatga cagcagtcgc tactttgcca taccacttcg ctccacttaa
    37921 tttgatattt ttagatagcc cgattaatcc agctacaaac ataaagcctt cttttatcaa
    37981 gaatagacca aacaacagcc aaagccacgg tcgataccat gccatagaca aaatcagaaa
    38041 aagttgtgtc aatttatctg caaacggatc aagcaccttc cctaagtctg ttacctgatt
    38101 atatctccga gcaatgaccc cgtctagata atcagacaaa cctgagacaa caatgaccgc
    38161 tcctgctata taaaatgact gtctcagcac aaaataaaga taaatcggca gcaaaaaaat
    38221 acggaatatc gataaaagat tcggaatcgt ttgtatttct ttacgccagt ctgtgcttaa
    38281 tttcatttta tcgccctacc tcctcagcac tcttttcttt ttattttagc ttacacgggg
    38341 aataaaacca atgatttcac tgtttttatt tttctttaca gacactgata ttatgagcta
    38401 atttgagata atcacttgct gttttcatcg tttactcttc tttcttcctg tcgtttgcgc
    38461 atttctttta ctagtacttt atgagctttt tttgatcctc gctctaggtc acgctgccgt
    38521 ttacgtgttt tataatcgat aaacagactg tctaaatcgt aacctgtcgg gtacagatct
    38581 tctgctgaac gcaccagacg aacacgctga cgaggcacgt ggattatttt tttgtctaaa
    38641 tatacatcaa tcgtatcttc acctatatct tcataaacta acgcttcttt ttggtattca
    38701 ttgacaaata tccgatcacc ttttgcaaag agctgacgtt cttttttatt ctccttccta
    38761 tttgtaactg ctgtcttagg aagaaattct tttttctgtg tccgataggc tttttccgcc
    38821 aagtaacgct ctgcttgatg gatcaatttc atcgacatat ccattttatg agcaatccac
    38881 aaagcttggc tttctcctgt ttcttccaac ccgcaactga tatttaggac gaagtgcttc
    38941 ccgatcgaaa gccatagcag ccggcacaaa atcttcatga tctccggcga atttcttgat
    39001 ttctccataa tgggtcgtgg caatgatcaa tgccccttgc tcatacatag attccatgat
    39061 tgcgatcgct agtgcagccc cttcatttgg ctctgttcca ctgccaatct catctaaaag
    39121 taccaacgta ttccttttta tctttttcaa aatagcagcg atgttttgca tatgaccaga
    39181 aaatgtactc aacgcatttt ctaaattttg gtggtcaccg acatcgacga aaatctcatc
    39241 aaagacagca agctctgtcc cttcttttgc cggaacttgc ataccgaaca tcgccatcaa
    39301 agtcaataaa ccgactgtct tcaacacgac tgttttgcct ccggcatttg ctccggtgat
    39361 aatcaagcct cgatagtctt taccgatttc aacatccaat ggtaccgcat gctcaggtaa
    39421 aaatggatgt ctgccttgct tgataatgat atgttccgat ttattgattc taggtgtgct
    39481 tccattgatt tctcggctaa attttgcccg agcaaagatg atatctaaca ccgtgatcgt
    39541 ttcgatcaat aaatcgatca cttcttcgtt ttctgcgata gcaccagtca atgccgccag
    39601 gacttgatat tcttcactga tttcttcagc ttttaacaac tgatagtgtt cattcaattt
    39661 agaaatcaca gttggttcaa taaaaaccgt cgttctttta tttgattcat cgataatcgt
    39721 tccatctatt ttatttttat agctggcttt gataggaatc gtatagtatt cgcctttttg
    39781 aacaatcatt gcttcttgga tcatttcttt gtttttcgga tgacgcaaga atttcagtaa
    39841 ttttgactgg atttcttttt cgattagttg taattgtttg cgtactttac gcaaattacg
    39901 tgacgcgtcg tcactgactt tattattttt tattttctgg tcaatcaact cttctacatt
    39961 tatcaaatca ggaagatgtt tgctataagc aaacaacaag ggtgtctggt attgattctt
    40021 atcaaaaaac tttgtgatca tccgactgct tctaagaaaa tccgcatatt cgatcaaatc
    40081 ggttggctgc agaatcaacc cttttttgac ttgttctgtc agtgcatcga tcctcggcaa
    40141 ccccataaat ggaacatgct gactgctttc taagatcaag cgtgcttctc ttgtttcttc
    40201 ttgacgatcc aagacagttt gtaggtttgt cgagaccgtc atatcgctga ttcgtttttt
    40261 gctatagttt ccaattgctc ttgcttggat ttcctttttg atccgatcaa attgtaattg
    40321 actaatcatt tcgttgtcca tattctctcc ttttcttgaa gagatacctg tcccaaaata
    40381 aataagggcc tagagataaa taaaaggata gaacacagct taaaaaagtc atgtcctatc
    40441 cttattagaa tacaaagata ttgttttcct gacaggtacc gtttcataag cacaccaaat
    40501 aaactttgtt tattggctat tcgacttact gattagatac cagctacact cacaaaacat
    40561 accccattct tctaggtctt atttattttc cacttaacta tagcaagaca cattttggtt
    40621 gtcaaacttt agtatgtttt atagatttac accattaatg aatagacacc taccgcgata
    40681 attgctccga tcattggacc tgcgattggt acccatgaat atgcccaatc tgaagttcct
    40741 ttattagcta ttggcaatac cgcatgtgct aatcgaggac caaagtcccg agcagggttg
    40801 attgcatatc ctgttggccc tcctaatgaa agaccgatgg ctaagatcaa tccacctaca
    40861 gcgaatacgt tcgttccagg tgcaaattcg ttttgtccaa aagctagcaa cccaagaact
    40921 aatacaaatg taccaattaa ttctgtgatc acatttgctg gataattccg tatagcagga
    40981 cctgttgcaa acgttcctaa aatcgcgctt tcatctttcg ttgcattcca atgtggcaaa
    41041 taagaaagcc aaacaagaat ggcccctgca aaagctccta aaacttgcgc aatgatgaaa
    41101 ggaacgacaa gattccaact aattgcccct gtcatcgcca tagctagaga gactgcagga
    41161 ttcaaatgag ctgggcccat gaatccagaa atatagacag ccattgttac agcgagtccc
    41221 caaccaaatg cgatgacaat ccagccagaa gcctgtgctt tacttttgtt taaattcaca
    41281 gccgcacaca caccatctcc aagcaaaact aggatcagtg tcccaataaa ctcccccatg
    41341 atttgagtca tatcactgtt catttatttc tcccccttta gttcccgcag atctgattct
    41401 gcaatcaatg cttctaaacg tttagtctgt tgcgccttct cctcttctgt ccattcaaaa
    41461 tatccagcaa ttgcatcgat cacaggttgc ttgatctcat caagctgatc tcgttcaaac
    41521 aacagatgat ttgtacgacg aatgagataa tcgcctgggg ccaaaaccat ttcttcttct
    41581 aaaccataac gtaaccgagc tgactcagcg agactcaaac caggataagg tgccatttct
    41641 tttgctaatt caaagatacg tcgggcatta gtcccgtaaa aatcagcgat ataagtagca
    41701 tcttcttcct ctaaaccggc tgctactcct tcttttgcta attctgtcac tgtttcttct
    41761 aatttagttg gatcgaagtt tccacctgaa atctgatatt ttttagagtc gatttctttc
    41821 gtctctattc cgtattcttc ttttaataat tggcgtatca atcgcaaagc tccttctgcc
    41881 atcttacggt aatcggtgat tttcccacca gataaagtca gcaaaccatc tggttctctt
    41941 tctaaagaac tacctctgga aatcgtagaa ggtgcttttt catcacggct gttttccaaa
    42001 tggttcaaca cgtcttctac ttcagcacga gaaactttgt tttccttata ttcacttact
    42061 gtatcgacaa ctttattgaa gctcttgtct gaaatcgatc cattatctcc accattgtaa
    42121 tcagaaccag aattaccaat caacagtgga cgaagtcctg cccagcttgc ttcgatatca
    42181 ttcaatgtga tatttgcttc tggatagcga tgattgatca catccaatag ataatctaca
    42241 tcttcttgtg tgactttcgg atccgtaaaa tctccttggt aatccgtatc tgtcgtacca
    42301 aagtaagtct tgttttctct tggaatagca aaaaccatcc gcccatcttg ctttcctgta
    42361 tcgaagtatg tgggttgcgg tacaggcagt tttttcgcat ctacaactaa atggatccct
    42421 ttagttggac gcattttagg agagactgga cgcgtaaaat tcaagtttcg tactttatct
    42481 acccaaggac cactcgtatt gatcactaat tttgctttga tttcgatcac ttcatctgtc
    42541 agcaggtcac gagctttgac gccaacgatt tgatcgccat catataaaaa gccaaccgct
    42601 ttcattttac ttactagata agccccatct tcggcagcct ttttgatatt atcaatcact
    42661 aaacgggcat cattgttgcg gaaatctaga tagacacccg cgccttttag cccttctttt
    42721 ttcaaaaacg gttctctttc caatacttct tctggtgtca gcgtataatt ctcatatttg
    42781 gttcctgtca catttgccag tttgtcataa aggtccattg ctactttgac cgagaacata
    42841 ttgaaggttg ttgctccttc gccctcatag attggcaaaa gcattggatc tggttttggg
    42901 atgtgtgggg caatactttg tacgactgca cgttcaccaa ctgtgtcagc tactacttcc
    42961 acatcaaatg tcttcagata acgaataccg ccatgaacaa gtttggtcga gcgagaggaa
    43021 gtcccttctg caaaatcctg catttcgatc aatcctgttt tgattcctga tgctgctgac
    43081 tggactgcca caccggcacc agtgattccg ccaccgatga tcaacagatc caattcttct
    43141 tgctgcattt tttgaatgct atcttgtctt gtcttgtttg aaaacatatg gatttcctcc
    43201 ttattcaaat gcttgggtag cttttactgc ttttttccaa ccgttgtaaa gtttttctcg
    43261 gcgctcttcc tccatttgaa cttcaaatag cttgccagct tcataaaatt cgcggatctc
    43321 gtctgtatct ttccaataac cgacagctaa tcctgctaaa aatgctgccc ctagcgccgt
    43381 tgtttccaag ttttctgctc gttggatcgg tacattcaag atatctgttt ggaattgcat
    43441 cagatattca ttgtttgctg caccgccatc tactttcaat acaggaatct tgattccagt
    43501 gtcatcttgc atcgtatcga tgatatctct cacttgataa gcaatcgact gtaaagtagc
    43561 tttaatgata tcttcttttg tcgtaccacg tgtcaagcca aacatagccc ctcgtgctgc
    43621 ctgatcccaa taaggagcac ctagcccaac gaatgcagga acaacataaa cctcatcttc
    43681 gcttgtagag cgtttcgctg cgtcttctga atcgctagct ttttgcaaca tttgaagtcc
    43741 atctctcagc cattgaatcg aagaaccagc cacaaaaata cttccttcta aagcatagta
    43801 aacctttcca tttatgccgt aaccaattgt cgtcaataaa ttatttttgg ataattgcgg
    43861 tttttcaccg gtattcatga caataaatga acctgtacca taagtattct tgaccatgcc
    43921 tggttcgaat gccatttgtc cgaataatgc ggcttgctgg tctcctgcca tccctgaaat
    43981 tggtgcttcg cttccataaa aatgataacc ttgcgtataa ccataaactt ctgagttaga
    44041 agtgactttt ggtaacatca ctcgaggaat atttagtaaa tctaaaatct cctgatccca
    44101 atctagctga tggatattga acaacatcgt ccgactggca ttcgagtaat cagtaacatg
    44161 tgcttgtcca tcggtcagtt tccatactaa ccaagaatcg atcgttccaa acatcaattc
    44221 gcctttttca gctcgttctt gcgcgccttc tacatgatca agaatccagc gtatcttcgt
    44281 tgctgagaaa taggcatcga tcactaaccc tgttttttcg tggaaaaatt cttgatagcc
    44341 ttcatttttt aagttatctg caattccagc tgactgtctg gattgccaaa caactgcatg
    44401 atagattgga cgtcctgtat ctttttccca tattactgtt gtttcacgct ggttcgttat
    44461 gccaatccca gcaatctgat ttggtttgat ccctgattcg atgaacgctc cggcaataac
    44521 tgactgtaca gaattccaga tttcatttgc atcatgttcc acccaacttt cttgcgggaa
    44581 atattgcgtg aactcttttt gagaactgcc gatgtgtctt cctttcttgt caaagataat
    44641 tgctcgtgag ctagttgtac cttggtcaat cgccatgata tacgttgatt ctgtcattgt
    44701 gattccctcc attatatgca aacgctttct ttaactactc ttatcatact actgttttaa
    44761 aaaaagaaac tgtcaaaatt ttattctttt tttctttttt tgacagtttt ttattatgaa
    44821 gctatttttt aatcaattct tttctttttt cagcgtctct attcttcttt tcaatcgaat
    44881 aatgtcgtaa ggtgacgcaa attctgacag ataatattca cactgttttt tgtttctctg
    44941 gcaaaatgtg atcaccaaat catattcttt accttcttca taacttccaa tctcaatacc
    45001 gccgattccc ctcagttctc tgatcagata ttgttgaaaa gcaatacgat aaatcggcag
    45061 actttctaaa tcgattccaa tcactactgg ttttgcaata taaaaatcga tcatcaaaag
    45121 aacattggca tactggatca tcaggtaact aagctcacta tcttcagctc ttttcctcgg
    45181 caattgctgc accgctgttt cctgtaattt cgccagtaac tgtgttaagg aatgcccgag
    45241 cattttctgc tgctgttcca aaagccgatc acgctcatag acttcgatct ttccaacaaa
    45301 gaaaaaccat tcattgttga tctgggaaat ttggtagcct actgcctttt cttctttgat
    45361 tttcaaacga cgaggacgat aatgccataa catcgtttcc cgaatgtaag tatctaaaac
    45421 agcggttggt aactttttgc tccgtgtcaa atcatactga taaaatattt cttcgtctac
    45481 tatcgaaaaa gaaacaaaaa agctgtaaaa catcattgtt tcgtatacat tcagttctgc
    45541 tgctgttcga ctaagatgca tcacgatgat tggatcgatt gcttgataaa gccggtccga
    45601 ctgatagagt aatttctttt ctttcaaagt tgcgtacgta cttttttcat tcaatagtct
    45661 ttttttcgta atcccgagcc agcaagcaat ctgtgcttct gctgatgccg taaacgttgt
    45721 ttctaataca cggtccaatc ctagaatgaa cggtcttttt tctggtgtat tttgcaaaaa
    45781 aagcggtcgc tgatcttccg ggatatattg gaatagttca taataaaaat atcgtatctg
    45841 tagctcttct ccgtataatt gaccgttttt gatttgtaat ccaaattcct ctaagagctg
    45901 gttcaattct ttgatctttc gaaaaacaga agactcactg atattaaacg ctgtcgctaa
    45961 tcgaacgatg gatacttgct gatgttctaa taaatagacg agcatcttaa acttcaagct
    46021 ttcctgcaat aaaaacaata gaattttttc caaacttaca tcttcgtcca acacaagcag
    46081 cgcctgatac tcatttcgct gtacttctac ttttttttcc atcattcggc caatcaattc
    46141 aatatcttcc aaatattgat caaacgaggc ttttgacagt tggacatgct ctctcaaacc
    46201 attgatagag gctgttcccc cttgcagaac aaggtttcgc aaaatcagga tctgtaactg
    46261 ctcttttctt tctaataatg cttcaatctt cataggcagt attcgccacc tttgaccaca
    46321 ccatatctgg cacacggtat tctggctgga aaaatagctc atgttcttct aactgttcaa
    46381 acgtccatcc ttcttctact gcaattgtca gttggtcaag aaggtggaac atctcttttt
    46441 tgctttggag ctgtgctcca agaatctttt tttcagaatc aaatagaaat ttgagtttaa
    46501 ctttgctttg atttattgct gtaagcttgt agtctacgta tcctgtttca attttttggg
    46561 gataaaaaat gctctcttct tctgtcaaac ctacgcttcc taagaaatag ccaaaccact
    46621 gattgccgac tggacgatag gttccctgat cctttgtctg tgagccacta attgtcttgc
    46681 tgacatttcg tgctgtacgg acagcgttat ttactagaga aacatacatt ttttcatttg
    46741 ttggacgaaa agttacttgg atcgcatcgc caatcgcata aacgtctttt tgagacgttt
    46801 gcaagtagtc atctacccag atcgttccat catcattcaa tgttaattgt tcttcccaca
    46861 tctgattgtc gggacgagaa tgattagcca gcaaaacaag atcatagttc tcctttcttt
    46921 tttccgtctc tacaatagcg gtttcatttt cggtcaaaga cacaacttgt tcttctaaaa
    46981 aaagtgaaag cccttgattt ttcatttcct ttactaacgg ttctgtcatt tcaggatcta
    47041 gataacgaaa aagaattgac tttcggcttt cgtaaagatg gatttgtttt tttcttgatg
    47101 ccaacccttc agctatttcg attcctgctt gtcctgcacc aataattgca attttctttg
    47161 cattcatgat cttttgctga agagccgtta cgttttgtat ctttttaact gggtgaataa
    47221 gcgttgattc tgcagaaata tttcgaaact tttgatttga accagtcgct agaatcaaac
    47281 gatcaaaatc caattttttt cctcctgcta aaacgacttt tttttctttc aatcctatta
    47341 ctgtcttttc tgtgtaaaca tggattcgat agcggtcagt cagttcttct ttcgtgatcc
    47401 aagaaagttg tttttcactg gtaacctttc cttttaatat caaattgaac ccgctgggaa
    47461 tccatcctaa cttcgattgc ttttcaatca aatatatttc acttgttggc atcaattggc
    47521 gaagataaat agccgcatga atccctgcaa acgacccgcc gacaattaca atgttcatct
    47581 gcattcctct ttcttataag gtgttctact gctaacttct caatcagaaa acacagtatt
    47641 cataactcgc tttcaacctt atgatacata gcgatgcagc agaatcaacc ctctccatca
    47701 taccgtgttc taggtctgct tctcaataat gtactagtgg acctaattga agagtatcat
    47761 ttctgaagag atgtttcaaa aaatgtactc ttataaaaga aaaaaactcg ccttttacgt
    47821 acctctgtcc aaataggaaa cagaaaagaa cgaaaagacg agttttagcg tgtgtaatgt
    47881 aatagttaga atagacacat catttatttt taattcttta atgcttcaac aactgcttcg
    47941 cctaattgat gagcagattg aggattttgc cctgtaagaa gccgcccatc aatttgaacc
    48001 acttctgaaa aagctgctcc agactgatag tttgctcctt tttctttcaa agcatcttct
    48061 gctaagaatg gtacttcatt cgttgtacca tttgcttcct cttcttcatt actgaaccca
    48121 gtcactgtac gtccagctaa aatagatttt ccagactcat ctgtaactgg caacaatcct
    48181 acaacaccgt gacaaacagc tgagaccagc ccttcatttc gatagatttg tgaagcgact
    48241 ttcccaagat ctattgcttc tggaaaatcc cacattgttc cgtggccacc agcataataa
    48301 accgctgcat aatcatttgc atccacatca tcaggacgta aggtatttcc caaggcccga
    48361 tttctgaaat cttcatcttc ataataggcc caatcgattt catccatatt caaaacactt
    48421 tgcggttcta aaggtacata tccgccttta ggactgacaa aatctgcatc gatcccattt
    48481 tttactaata catcataaaa atgagtcagt tcacttaacc acaaacctgt aggacgctcg
    48541 atcttttcgt acttttctac atttgttaat acgatcaata ctttcatttt cccatcctct
    48601 ttctataaaa attgcgttat tgataacgtg ctttcaagaa attatataat gggcttggca
    48661 aataaagctc tttatatgct ttttcaaaag gtttcgttac aaattcatcc tcacggtttt
    48721 ccaataattt ctctgcccag tcaggatcga cgatcatttg ctgtccaatt gctgcaagtt
    48781 ctgcatattt caatacctgc tctacatctt cgcgattgcg gatcccaccg acaccaacta
    48841 aagggatctt tccttgaatc gtgttatgga ccacttcaag aatcgattta tcgttgtatt
    48901 tatcagaacg agctattcga tcatacatat ttaatgaaac atgcagataa tctagttttg
    48961 actcacgtaa tcgttcaaga agccagatcg tgtcttcaaa acggatacct ggtgtttcat
    49021 attcttctgg tgagaaacga taccctacga tgaatggttt tttagcatat tgttcaaccg
    49081 ctgagaaaac cgttgttaat aattcttcaa taaaacgata acgattttcc aagctaccgc
    49141 cccattgatc ctctcgtcta ttcgaatgcg gagagaaaaa ttgttggatc aaatacgtat
    49201 ttgctccatg caactcgatt ccatcaaatc ctgattcaat cgctctacgt gtggctttgc
    49261 caaatgcttg gattgtttca acgatttctt cttcactcat tgctcgtggt gtttccgcat
    49321 caggacgttt tgctgcaact gcacttgcag atacaggctg ttctccagat aaagttgctc
    49381 gacttgtcat tcgtccaccg tggaaaatct gaataatcgc ttttgcgcct tctccttgga
    49441 ttgctttcgc tagctcactt aatcgaggaa tcatttcatc atgagcgatg ctgagttcac
    49501 ctggccagcc ttttccgata tcttggacat ttgcagcacc agtaataaca gcaccaagac
    49561 ctttagaacg ttgtttataa taagtgatct catcttcggt gatcactcca ttgaaaaagc
    49621 tgagttgtgt cgtcataggc gacagcatca atcggttctt caattgaact cctgaaggta
    49681 aagtaattga ttgatcaaat tgtgacattg tcattcctcc cataattcgc tctgattaac
    49741 ctatcgagaa tcatctatca ttaaaccact cgattagttg tacatgcaac taatttgctt
    49801 aaaaaatttt attttttttg taacttatct gttgcatcat tcattgtctg ggctaattct
    49861 ttacttgctt catttcccaa aactgctttg tatgactgat gaagttcttc ccagctacca
    49921 tcgacttcct tagcaaattg gcgtccttgt tctgtaagat aaactaatga caagcgacct
    49981 tcgctaactg ttttgacgta tcctttatat tctaattttt caacaaaacg agcaatcgtt
    50041 gatggggcgg aatagagcat ttgtgcaata tctttttgtg gtattccatc tttttctttt
    50101 aatatcaata aaataaaacc ataagttggt gccagacctg tccttctaaa cgcttcatca
    50161 gctaatttat tcaacatgcg atctagcttt ttcacgctaa agaaaagaca ttcatctaac
    50221 gctttttcaa ttggcttttc catttcatcc cctccattgg cgtacatatc atagtatgat
    50281 ttttattttt tgtcaaaaaa cacggaataa tgtccgcctc ctctgtctag atttgaaaaa
    50341 aagaagtagc catcttcaaa gaatttcggc ttgttttcga caaaactcct tctggaacac
    50401 cgtgaatcag aggttgtgaa aaagtcgttt agttttgagt atgaagaaaa attttgtgaa
    50461 aatggctttc cacatttttg cataaatttg gcttaatacc gaaaaactgg cttttgaaca
    50521 ccgtttatat cagaggttgt gaaaaagccg tcctgcatca agtaataaga acagccaaac
    50581 aaaaatagtt cctcatattt tgtcgttttt tgagctaatg ccgatggtca gctgattgta
    50641 caccgtttat tgagaggtcg tgagcctgcc gttgaactcc gagtcacaag gagcaatttc
    50701 aaaaaacagc ttctcatgtt ttatgactat ggtgacttgt gatcgaggag ttggcaggag
    50761 aatactgttt attcgtaaag aaggggctgt gacaagactt ttgtcacagt cccttatttc
    50821 cttttattat aggatttcgc catttgattg gatgacttgt ctgtaccaat caaatgattt
    50881 tttctttaac cgtttctttg tgccattccc ttcgtcatct tgatcaacat agatatatcc
    50941 ataacgtttt gacatttgtg aagtagaagc gctgacgata tcaatgcagc cccacgacgt
    51001 ataaccaaat aaatctactc catctttgat agcttccttc atctgctcaa tatgctgctt
    51061 caaatagtca atgcggtaat cgtcttgaac ggttccatct tcttctactt tgtctatagc
    51121 tccgagtccg ttttcagcta tgaacagtgg tttttgatac ctatcatata attgattcaa
    51181 agcaattctc aaacccttag gatcaatctg ccagccccat tcacttgatg gaagatacgg
    51241 atttttaatt cctccagtta ttaagtttcc ttggacccct tcccaatttt ctggatgagc
    51301 actgactgca ctagacatgt agtagctaaa agaaacaaaa tcaacagggt aggctgctaa
    51361 gatttcttca tcacctactt ctatttttaa gttgacacct ttttcttcaa acattcgttg
    51421 cgtatacgct ggataatatc ctcttgcttg aacatcacta aagaacaaag aagttctttg
    51481 ctgctcaatt gttttttgaa tatcatccgg atcacaagaa tacggaacaa gaagctgata
    51541 gctgatcata caaccgactt gtgaaccttt gataatttca tgacaagctt ttgtagctaa
    51601 agcacttgct ataaactgat ggtgtgcacc ttggtatttc gcttgttcca aatggggatg
    51661 gttttcttca attactccta cgctaacaaa agggtgatgt tttacacagt tgatctcatt
    51721 gaaagtcatc caatatttta ctttattttt atagcgtttg aatactgttt cagcgaaacg
    51781 aacgtaaaac tgaataagct ttcgattagg ccatccacca tattctgtaa ccaaatataa
    51841 cggcatttca taatgggaaa gagaaacgac tggctcaatc ccatatttta gacattcgtc
    51901 aaacacttga tcataaaaaa ataatcccgc ttcattgggt gtttcttcat caccatgagg
    51961 aaaaattctt gtccatgcga tagacatccg atagcattta aaacccattt cagcaaataa
    52021 acggatgtct tctttaaagt gatgatagaa atcatttccg tgtcttttag gataataata
    52081 cttgctgttg ggatcctgcg ccgcttttac ttgctgatgc gtcatcatat gccattgatc
    52141 tgtccatttt tctttaggta aatcgctgtc gaatgtatac acatcagaca cagataatcc
    52201 tttttcatct acatcccatg caccttcaca ttgattggcc gctgtcgctc cgccccataa
    52261 aaatccttct ggaaattttt tcatatcgat ccccctaaac ttgctagttt tcttgtttga
    52321 ttatttttct tatgactagt aatgatattt ttgaatctac tactatttgt aacaataacc
    52381 attgtggtcg gatcatatcc ctctttgatt aattcttgca aatctacttc catcaataaa
    52441 tcgcctttac gtactttttt tccatcagat actgcacttt gaaagtattt gccatttaaa
    52501 ttcactgtat caataccgat atggatcagt atctctgctc cttcttcact gactacccca
    52561 attgcatgtt tcgttggaaa aactgttgaa acttccccgt ttacagaact gtagattttt
    52621 ccgtccgttg gaatgatcgc aaacccttct cctaaagatc cactagcaaa tgttggatcg
    52681 tttacttcag ataaatcaat tatttcacct gctgcaggag tacttaacac tatatccttt
    52741 gaggattctt cttcgatttc atccttgtag ccgaaaagaa aagttagcat acaggaactg
    52801 atcatcgcta agaaataact agcaactagc caaacaaatc cttcaccaat ataaatcggg
    52861 aaagacaaaa tacttcttgt agcaactgcc aaagcaccgg ctcctgcggc accagctact
    52921 ccaccaaata tagctccaca tatacaagca atgtaaaatg gtcgtttata tttaagagtc
    52981 acaccataaa cagcaggttc agtgattcca aataatgctg tgataaatgc agataaagat
    53041 aaacttttta ctttcttatt tttcgttttc aaaaataccc caagtactgc tcctgcttgc
    53101 gcagctacgg cagtcataca tgctggacct agcgtatcac gaccgtagaa agaaagattg
    53161 ttggtcatta ctggtacaag tccccaatgt aacccaaaaa tcactaacac ttgccaaaat
    53221 ccgccgataa cagcaccaga taaaacaggg ctaaagttat aaacagctaa atatccagct
    53281 gcaatcagct gacttgcata atctgcaatt ggacctacaa ctaataaaat aattgggacc
    53341 atcacgatga tacaaatgaa tggtgtcaat aaatttcgaa tactttcgtg caagcttttg
    53401 cgaatcttag gctcaatata agaaagaacc catacagcta aaattgctgg aataaccgta
    53461 cttgtatacc ttgctagaat cacgggcatc cctagaaatc gcaaggttat gctatcacta
    53521 tatgcactga caatcgtagg ataaacgaga gctgctgcaa taaccatagc gatgaaacga
    53581 tctgtattga acttttttgc agcagtatat gccaaaaata taggcaagaa atagaaaaat
    53641 gcatcagctg ctgcattcaa aatgatataa gttcctgatt cagtagttaa taaattcaaa
    53701 ctcgtacata aggcgagtac tcctttgatc aaaccacttc ctgccaagac gcctaaagtc
    53761 ggggcaaaaa cagcggcgat catgtctata aaagagttta aaatgccttt tttctcagtt
    53821 cctctctttc cactagatgc atcatcttct agtttcattt gtttggataa tacgtcatac
    53881 acttcattta ctgcatttcc tactacgact tgaaacattc ctccagcttc gactacagtg
    53941 atcacacctt ctagttgctc taccttctcc ttatctgcct tcttattatc ttttagggta
    54001 aagcgtaacc ttgttgcaca atggtaaaca ttgctgacat tttcttcgcc tccgacaaat
    54061 cggacaatat ctttcgctaa ttgtgaatag tccattttta cttccccctt gttaattaat
    54121 gccgtagcaa tccttcttac aagataagta tagttcttag aaaacggttt ctccattcaa
    54181 ttagtacccc ttctaatagc gaacgaaact gtacaatagt gtgtatggtt tacttagccg
    54241 agcaaatcac tcactgcttg aaagaaaaca agtatatcgt ttctcttata gtcaaaggag
    54301 acaaaaaaaa cacgcttgat cttcctttgc tgatgtataa gtggtatctc aagctgatac
    54361 tctgtgattt ttgtttctac ttggcttttg ataagacagc acaacaaagt ctcggcattt
    54421 aacggaagca taactaggtc agaactagga gagattagtt gctgaatgga atctatcttt
    54481 ttatggattt ttatttgatt ttcttctttg tagtaatgat caagcagttt tgttttcttt
    54541 ttttcttcaa ctatggtcag cttttcacgc aaaatctgat aaatctccca agattcaaac
    54601 tcatctagaa cactaggtag ataaatcaaa tcatcccact caaaaataag ctcttgctca
    54661 gtagtcaaaa ttgcatcata ttccgttatt ttgtttgatt gatttttgaa aagatacact
    54721 ggttctatcc ggcattcttt gatccacatg ttcatacttt gttgaatctg tttttttagt
    54781 gtattcaaaa tactaattgg ctggttacta acaatcagta tctccccttg ttttcgaaaa
    54841 ggattcaacg cttcggcaag atacaaaaca aaataagcgc tttcagctaa attgggacgt
    54901 aattgaaaat gtattgggaa gaaacgccga attaaataaa tttctaaagg ataacgggag
    54961 atgatctttt gtacaaaatg attcatggta ttatgtccgg catccatcct cgttttcatt
    55021 tgtttgatat gggttagcaa aaattctgtt tcatggaaca ataacgaaac aggaagatct
    55081 aaaaaacgaa taatcgtttg ctcaaatgct cttagattct tctctatttc tttgtttttt
    55141 tcatcattaa tcatcaaata atttaactct aatagtcgat tttcaattgc ttgtttttct
    55201 tgattggaaa aagaataggg tagttctgat gataactttt ttgtgagggt ctctaccatc
    55261 ggaataaata atgtatacct gtttgttttt tctaaaagtt cttctcttcg aagaatggtc
    55321 aaaacgagat accgagaaaa tcgtatgaag tcctctcctg aaacaatata attatatgat
    55381 ataagaacct cttttaatgt cttttggaca gtcggcatga tattggaaaa aattgtactg
    55441 atttgttgag gaaaccttgc gtgttggatc accgtttctg ttccaacaag agataagaaa
    55501 attctttttg aattttcttc agcaatgatt tttagtcctt ttgtcgacga aacttccagt
    55561 tctaatttag gattgcgctc gatccaacgc aaaatcgttt ttatttgtaa tgatacggct
    55621 gtttttgaaa gaaagaaaat gtctgctatc ttttgcatac tgacataccc tttttgaaac
    55681 aataagtacg tcacaatagt tgaagctcgg aaatccatgc tggaatagat tttatgtttc
    55741 gtcacctctt gtatgatttg ctgtttagtt tcagattcaa ttgattctaa aaaataacct
    55801 gaacgtttat ttgactggat aatgattttc ttctcgtaga tagcaagcat atttttgatt
    55861 tcagtttgga tcgttttctt gttggtacct aaagcttcag ctaattcttc gcttgttatc
    55921 caatgattct tatttcttaa tactacaaga atttggagtt gtcgtttatt taagtccatt
    55981 tgcttacacc tcctctttgt tataaacata caggaaaaac ctctcaaatg acaatagtca
    56041 cttgcagagg tttaaaaatc atttgaagaa agatctatat tcaaggtttt taccattaga
    56101 ctggataatt tgtttataat attcaaaact atcttttttc aatctcctaa agcttccgct
    56161 cccatcattt tgtctatcca cataaataaa gccatatcgc ttcctcattt cccctgttcc
    56221 tgccgatacg atatcgattg gtccccacca taagtaacca atgatttggc aaccatcttt
    56281 tattgcctca gcaatttcct gaagatgtat ttttagatac tcacaacgtt ttggatcgtg
    56341 gattcgattc aattcatctg gtgcttcatc tagtccaatg ccattttcaa ctatgaataa
    56401 tggtaattca taacggtctg tcaattcatt catgacataa cgaaacccta gaggatcaat
    56461 tggccaactc caaggttcgg gagatttttc cttcaaaaag ggattatcga aaccatttgc
    56521 tccgccagta tctacgcgca tttctgcttc ttttttatag acagcgctac gataataact
    56581 aaatgcaata taatctgccg tgttagcttg caagcactgt tcgtctccat ctaacattac
    56641 tggtgcacag tcttcttcct tccatgtcct gtaaatataa gctggataat gccctcggca
    56701 catgacatcg gagaaaaacc atgaattgcg tttaaattgc agtgaaccaa atacattctc
    56761 tggatcacag tcatatgcat aagtcgctaa tgcagaaaag ctacacataa tacccatctt
    56821 agcatttgga tcgattttct ttaataattg agtagcacgg gcgttcgcta gaaacatatg
    56881 atgacaggct tgataaatca ctttttttgt tagtgtagca atgggttcaa gtttatctct
    56941 agcagggaat gtaaccaatc cagcagctgc ataaggcata cgaaaagcat tattgatctc
    57001 attaaaggtc atccaatatt tcactttccc ttggtagcgt tcaaagacgg tctgcgcata
    57061 aaattcaaaa aagtcgatta attttcgatt ttgccacccc ccatactctc ctaccaatgc
    57121 taagggggtt tcataatgac taagagtaat tactggctcg atatcatatc gatttaatgt
    57181 attgattagt ctgtcataaa atacgagacc agcctcattt ggcttctgtt cgtctcctct
    57241 tggaaatatt cgtgcccatg agatgcttgt tcgaaaagct tttaaaccta actccttcag
    57301 taaacgaata tcctcttcaa atcgatgata aaaatcaatc gcttcatgag aaagataatg
    57361 gagtgattcg tctaaagcaa tctcccaaat ctttttttct tcattccact gaatactagg
    57421 atgctggttc atgatcccga taagcgtatc tgtcacatta ggtaatttgc cgtcttccaa
    57481 ccatgcacct tcacattgat tagcagctac tgctccgccc cataaaaaat tttctggaaa
    57541 tatttcctga tccataacta tctctcctcg ctatcaacaa tttcttactt gaagtatagc
    57601 tcgattcatc aaaacaagat attctaacta tttcggcaga agagaagaaa aaaattgaat
    57661 gctactcttc atttttaata tttatatata acaaatacat aagacgatcc ccgaaagaaa
    57721 acactcgtta accatctaag caaacgattt attttacttc caacacaaat gcttcataag
    57781 gtcgtaaatc aactttgccg tctggtaact gtttcagagg ataatttgac acaataaccc
    57841 ttaaagcttc acgtggaata gtgaacgtct gcttttcact ggacatatta caaataacga
    57901 gccattgctg gtcttcatat atgcgagaat aggcaaagac atgctcaggc gtcttttcca
    57961 ctaattcata gtctccccag acgatgatgt catgttttct acgcaaatcg atcaatttct
    58021 tgtacatata aaaaacagaa tcttgatctg ctaacgcttc ttctgcattg atttctttat
    58081 aatttggatt gactcgaagc catggaattc cagtagtaaa cccagcatgt tcacgggcgt
    58141 tccattgcat aggcgtccta gcattgtctc ttcctttcgc cttgattgat tccaagattt
    58201 cctcttttgt aaatccagaa gaaatccttt cgttatacat attgattgtc tcaatatctt
    58261 ttgcttccgc gatatcatcg attgggcaat tcgtcatgcc taactcttct ccttgataga
    58321 tataaggggt gcctttcatc atatggagca agatagcaaa catcttggca ctttcttttc
    58381 gatacacatc gtcatttccc caacgagaaa caattcgcgg aagatcgtga ttattccaaa
    58441 ataaactatt ccatccttca tcacctaaag atgtttgcca tttggataat acttttttca
    58501 gttcagagat actcaagggt ttcaaatccc atttgtcttt gccttcttgc tgatctaatc
    58561 ctatgtgttc gaattggaag accatggata attcatttct tttaggattg gaataaagtt
    58621 tagcgatttc ggtagttgct ccccatgttt cgccaacagt catcagattt ttgtcaccaa
    58681 aagttgcttg attcatttcc tgaagatact catgcagttt aggtccatta cttgtgattt
    58741 ctttatccgg ttctttgcca atcatatcga tcacgtccat acgaaagccc ccaatacctt
    58801 tatcgatcca gaaattcatc atttgataaa tctcttggcg aacctgttta tttttccaat
    58861 tcaaatcagg ttgacgcttg ctatacaagt gcagaaaata ttgccccgtt ttttcatcca
    58921 actgccaagc agaaccactg aatattgagc gcaatctatt aggctctttt ccattgaccg
    58981 gatcacgcca aatataatag tcccgataag gattttcttt atttttacgg gcctcaatga
    59041 accacggatg ttcatcagaa gtatgattca ccaccagatc catgatgata cggatatttc
    59101 ttttctttgc ttcttcaatc agttcttcca tttcttccat cgtaccgaat tctgccatga
    59161 ttgcttgata attactgata tcatacccat tgtcatcatt cggagactcg tatactggag
    59221 aaagccaaat tgcatcaata cctaaatacg ccagataatc caacctggaa ataatccccc
    59281 gcaaatctcc aataccatct tgatttgaat cttgaaaact tcttggatag atttgataaa
    59341 caactgaatg gtgccaccat ttttgttcca tgattttcct cctataaatg gatgtttcta
    59401 ttttttgctt aatcatttat tttagtctct ctcactaaaa attgtaacgc tttcaaatag
    59461 ctattgcacc ttgttatcgg taacagaata aaacaaaaaa acagctgagg aaattccctc
    59521 aactgttttt tgaaaggatt gatttatgca gggaagaatg ctgatttcac aacttcttta
    59581 taaacggtgt tcactcagct gtcccttggt attagctcaa aaaacggcaa aatataagaa
    59641 actatttttt cgtttttttc tctaatactc aggccagaac gctgatttca caacctcttt
    59701 atttcacatt caatatccac atgaatccca caaatacaaa gaacagacca tacatgattg
    59761 gagatacttc ttttcctctt ttagctgcta acatggtaat tggatagaag ataaaaccta
    59821 atgcaatccc atctgaaata ctataagtca aaggcatgcc gatcaagatc aagaatgaag
    59881 ggatcgcaat ctctaagttt ccccattcga tttgtcgcaa agattgtgcc atcaatactc
    59941 ctaccataat caatgctggt gcagttactt gtgaagtaac aactgatagt aatggagaaa
    60001 agaacaagcc taacaagaag aaaatccctg tagtaatagc tgtgatacct gaacgtcctc
    60061 caacggcgat accagctgat gattctacaa atgcaccaac aggagaagta ccaaataagg
    60121 aacccgcaag catagctgtt gaatcagaag ctaaagcttt ccctacacga ggcatcttgt
    60181 tgtctttcat gaaacctgct tggtttgcta atccaactaa agttcctgct gtatcaaaga
    60241 aagtcacaag caagaatgtc agcactacga cccacatttg aagagaattg atgtccccta
    60301 catgttttaa tgcaactaaa aatgttggtt tcaaacttgg tgctgcggca acaatttcag
    60361 aaggcattgg aatcaaacct gtgaccagac ctaaaatcgt tgtagctgcc atcccgatga
    60421 agatgccacc tggtacacgc cgaacaagca agatagcagt tacgactaat ccaaaaatcg
    60481 tcaaccaagt agaaccaacg ttgagtgatc ctaatcctac taatgtagat tcattcgaaa
    60541 cgatgatccc accttcgctc agaccaagga aagcgataaa tagaccgata ccgccagaaa
    60601 tcgcatattt caaatctgct gggatcgcat caatgatcat ttcccgcagc ttgaagattg
    60661 tgatcaaaat aaaaatcaaa gaagcaacga atacgccggc taatgctgtt tcccatggaa
    60721 tccccattcc taaacaaaca gaataagcaa aaaaagcatt gatcccaaga gcaggtgccg
    60781 ttccaattgg ataacgggct aataccccca ttaaaacaca gccgatcgca ctggctaatg
    60841 cagtcgcagt aaacactgct ccttcatcca ttccagaagc acctaataca gttggattta
    60901 caaataagat ataagccatt gagataaatg ttgtaaatcc tgccagtacc tcacgtttta
    60961 ccgtggtgtt caatttttct atttcgaagt aggaaagtat tttttccttc atcttccatc
    61021 cccctaaatc cgaacattat aaataaagtc cgttaaaatt tcgatttatt aataaaacaa
    61081 cgtgttttat catatactaa aacaaaacat aagtaaacaa aaaattcgga ttttaagcaa
    61141 aatccgaacg ttccatctga ttaaatccct ttatctcaaa gttcatgaac attcactttg
    61201 gattacaaga atttctttat tgtgttaata aagtttcttc attctagtct caaaaatcaa
    61261 tagtgtgatt tgattttacc atttcgtttc tgttcttctt tgatgaattc ttttgtatcg
    61321 acaggattca gattatttag tccgccaaaa cctgtatcga ccatttttcc ccagcctttt
    61381 gtcttctttt ttcctgattt gagattcttt gctcgaagct tctcactatg ttttgctcgc
    61441 cgatacttca aataaccttc taatcttccc attagatcca ctgtttttcc tcctttttat
    61501 tcaatcagac gactcagctt ttcctataac aaaataattg ccttcagggt cagaaaaatt
    61561 gaaagtcaag atgccattgt tatctgtgat ttctcctggt tcttccatgt tctcgtagat
    61621 agtttcaaat tcatccgtaa aaaatactaa agaaggcggt ggtcctaata cctctggtga
    61681 aaacttctgc acaaatactt ttgggaatac gcctaattct aattcttttg agatgctgag
    61741 tacaatactc ttaaattctt ctggcaattc gattgtttca gaaatttctg caccaatttt
    61801 ttctacaaag aatcggcaaa tcatctctac gtcattgaca taaagcatca cacgtgtttt
    61861 ttgtatcatc atttgaccct tctttctatt tgattttctt tcctgtctat aaactgattc
    61921 cattatatca cgaaagcgca aacaccttgt gtttgatact attcaagaaa aataaagcaa
    61981 tagtttctag gattgctttc ctccacaaac tttttttaca aaaaaaaaac ggcaatgaat
    62041 gccgctcttc ttatttcgat tcttttataa ttctgcttct gattcatctt tggttagtgt
    62101 gtgtctaaca ctagttacgc caaatgtttg atcgattttt tccttcaacg cttcgattgc
    62161 ctgtgcgcca cgtgccgctt gttcagcatt gatggattca atgaccagca ccgctgcttg
    62221 tgtttcttcc gtcagcatcg ttctttgggc ttcacgccag ttgagacagc gacagatcgc
    62281 tccttgttca tcatagtaga tgatttcttc cggaagtgcc ggagcatcgg aatctgcacc
    62341 taaaggcagg aatgattcgc caccttgggc ttttcctaaa tgcagatttc ctgcgatctt
    62401 ttcgatatct tctccgccgc aaggtacgcc aaaagacaag gaaatactat tatagatatc
    62461 tacaagcgga ttgattggga aaaattcccg ttcttgattt gcacgtttca gtaaagcttc
    62521 aatagaagaa cgtgcacctt ttttagtctt gaatgtggta aatgcctgac gccactgagc
    62581 aatgacttcg ttttggctga atgtttcttc tgtaaggtaa tttcttgctt cttttgctgc
    62641 ctcattcaat aattgatgat acgtttcttc actttctttt gttacgtggt tatcgatacc
    62701 atttacggat aagatttcga tggttgcttc aggaaatact tcccagaaat tggtatccac
    62761 tattactttt ttcatctgtt cacacctctt gattagaata attgcttgat gaatagatag
    62821 tctgtttggg gatcatctcc catataaaaa acatgttcgc tgtatttgat aaatcctttt
    62881 ttacgataaa aagcgagcgc aacatcgttg tgttcccaaa caccgagcca aatggctgtt
    62941 ttttgctttt cttttgcttt ttcaatagct gtatctagta aaaatgtacc gataccttga
    63001 tgtttaaatt tttttcgtac atagattcgc tcgatttcta aaaaatgatc agccatttcc
    63061 tcagattgac tcgttcctgt atttagtttc aggtaacccg caggctcttg atcgatactc
    63121 actaggaaaa aatcggtttc tttatctttt agctcttctg taagtttttc tagtgcataa
    63181 gcttgtgtca agtaatcagc taaatgctcc ggtgaattat cttttccaaa tgtatctgta
    63241 aatgtttcga tactaatttg ttgtagtaag tctacatctg aaggcgtcat ttttcgaatt
    63301 tcgtatttat tcacggcttc ttcctttcaa tagatccttt tattgccttt tttgacaaaa
    63361 ttccaatctt cgctaacatt cttgcatact ttatttaaaa gatattccaa ttgttttgct
    63421 tcttcgtcag aaagaccatt taatgcgact gcattggagt agttgttttc tcgaatgatg
    63481 aaaggataga tttctgctcc ttttttagtc ggatagagtt tcttgatttt tttattttct
    63541 ttatcttcca aacgttcgat catcccattg gattctaatt tcttgattgc tcgggcagtc
    63601 gttgtacgat ctactttgat catttctgct agtttttctt ggatgattcc aggattttcg
    63661 caaatacgta ctaaataaag atactgtcct cttgttaaat cgacttcttt gaattcgata
    63721 ttgctaatag aatctagcgc tcgggcaatc attccgatgt ctcgaaggat ttcagccatt
    63781 tcatcccacc tttattttgt ttgtaagact agttcacctc tttttgttgc aaatgcaact
    63841 attttcaata aaaaaatcaa gttctttttt aataactagt aaagtatatt cttttcttct
    63901 tttacgaaaa agctcaatca caatagatct ggtttttgtt ttctaaaata agtaactgtt
    63961 tcttcgttgt tcgcttttct gatagctttg gcaactcttc tatactaaaa aatcccatgt
    64021 tggaagtttc attattctca ataaacttgg cctcttcacc gtggatcgca caagcaaaaa
    64081 caagtttata atactgaaaa agctgtggaa tatctttttg tttattcgta tcaaaaactg
    64141 ctcgcaactg agtagtttct actgttaatc ctgtttcttc atagacttct ttacgcacat
    64201 tttcttcagg agacaatcct atttcagcaa atccaccagg aagtgaccat tctttcgtcc
    64261 gtttatcttc gattagtagg accttgccat cttttttgat ataggcacga acatctactt
    64321 ttggtgttgg atgtccttct ccaatcgttg tgagcgcttt tatttcttcg aaagtttctt
    64381 ttcctatttc actgatcagt tccaatgaaa tcgtccggat ctcctcaaaa cgctctttat
    64441 caaatacatc ttttccataa tatagaccag cttgagaaag tgcgagtagt cttttgtatt
    64501 gagccaactt cttgttcatt ctcttccctt cttttttagc aaatgtatga ctatgtaatc
    64561 aagtatagaa gactggttta attacggcaa ataaatataa tgaaatctgc aataaataaa
    64621 agaatgttaa aaaactcgag aacaaaagac attcgacttc tctggggctg tgaataaatg
    64681 gcgttcaaaa gcttgcatct gcggtaataa gccccaccaa acgaaaaata tgacgagcta
    64741 ttcttgtctg gctgttctta ttaattgagg caggacagct ttttcacaac ctccccacta
    64801 aacggtgttc tcctaccaac tcctcggtca caagtcacaa tattcatcaa acatgagaag
    64861 ctgttttttt gaaattgctc cttgtgactc ggagctgaac gacaggctca cgacctctcg
    64921 gtaaacagcg ttcaaaagcc aatttttcga tattaagcca tttctttgtt acttattgtc
    64981 attgatttct ttttctaatt tatccaatag tggagtgtaa tccacttgag tatagtctgt
    65041 tacatcaaat tcatatagtg ttcctaactg gaatttgtca tattttcggt cggcgatgat
    65101 ctttttgaat gcttctttcg taatatgatt ggtccctttc gttcgatctt ccaatacgtc
    65161 accatagtga taatgatcca tgatatagct caaatgacga tctgattcac gatcacgttt
    65221 gtaccgacgt tgccataaca cttcaaagtc tgctgttagg cgaatcgtga tcacctgata
    65281 tccgtatcta tctgctaatt cttggagtcg tgctttttgc ttatcactaa aaggatactc
    65341 ggacacaatc actttctttc ctgcttccat atacaaatca agtgctttgt aatagaaagg
    65401 ataaactttc gtcctttctt gttccgcccg ttcttccaaa ttgttaaacc cgatggattc
    65461 tgcatagtat tccttgattt catcgagcgt gatggcatac atatctggaa atcgttcccg
    65521 tagtttattc ataagatatg tttttcctgt accaggacta ccagccagaa gaatgaaata
    65581 ctttttcatc tcatttattt cctaagttaa ttggactttc caagttcata aacagtccaa
    65641 tcttatgctt gacaatttct tttactgctt catttgctgg aatcacatta tggcgaagag
    65701 acttgttgat ttctgtttca ctaaaggttt ctttagctac tttcgtccag ttgactaaaa
    65761 gctccgttcc aacattgaat ttgcggatcc cgttattgat caattcagga taatcttctt
    65821 ctttcacacc tgttcctcca tggataacta gggggacatc aactactgca tcgatttctt
    65881 tcagcaaggg aatgtttact tctgtttttg atttgaattg tccatgattc gtaccgatag
    65941 caatagctaa tgcatccacg cctgtagctt ctacaaattt cactgcgtct tccggtcttg
    66001 tatatacctt gtcattttca gcgacatgga ttccttcttc tgtaccgccg attgttccaa
    66061 gttctccttc tacggaaaca ccgtgcgcat gggcatattc tactactgct tttgtcttca
    66121 ggatattttc tttgaaaggc agatgggacc catcatacat gacagaagta tatccgctgt
    66181 cgattgcttc tttgatatca tcaaagtctc gggcgtggtc taagtgcaag actgcatcaa
    66241 ccatttcttc ttctgcaata gctgtcatta cattgactaa cacatgcatg ccgatatatt
    66301 tagcggtatc gacacttgtt tgaataatga tcggcgaacc catttctttt gctgcgccta
    66361 gcatagcagg aagcatttct aggttatgcg tattaaaagc tccaactgta aaattcaact
    66421 cttctgctgt ttttgttact tctttcaatg ttgtatacat cttttattgc caccttatct
    66481 ttttatttgt ttcctttgat cgttacttgt tttgcaattt gtcgcatctc atccattttg
    66541 cccatgtagt aatcgatccc ttgatcatca ccagcacgag cagcatctgc acgcttctgg
    66601 ttatagaggc tcatcagttt tttataccca tctccgatcg tctgtttatt ttccaaactc
    66661 ttttctaaat attggatggc ttgatcgttc acatacaatt cagaaaattt aagtccaact
    66721 tgttcataaa gtttcgctaa tttatgctta tcttgggtag tggaaatctc ttgtttcaat
    66781 tgtgtaatct ctgattgaat cttttcggtc gcttctggcg atagggcttt ttcttctttt
    66841 gccagaactt ttttcggttc tttttctttt ttcttaaaaa atccaaacat cattttccct
    66901 actttcaatt aaaatgcttt caaaactggg cttaagatcc aggcatataa gagtgctgcg
    66961 gctactgtat ctacatcggt tgctgtagca ttgttgaatc ccatcgaatt aaagatcgtt
    67021 actagcaaag cgggcaataa tgtaataaag aaaccatgag caataccgcc aatgatcgct
    67081 cctcggcgtc cgccaactgc gttgccaaat atccctgctg tccctccggc aaagaaattg
    67141 gttagcatac caggtaaaat catcgctaag ccgaacatgg gcaaagtaaa catggcaata
    67201 atcgtaccaa tcgttgtcgt aatgaaacct aaaatgacag catttgggct ataaggaaag
    67261 aaaactggac aatctaaggc agggatcgca tcaggaacta atttcatagc tatccctcga
    67321 aatgccggca cgatttcacc taacagtaga cgtacaccag ctagcaatac atacacaccc
    67381 actacgaatt ggatggcttg taaaaaggca aacatgatat agttttgatc tcctgataat
    67441 tcagaagcga atggttcgcc tgcaaataaa acagtgatga tatatagcgg gaccatcacg
    67501 accatgacag acaaataagt atcttgcaga aattcgaaat gtgtcggcaa cttgatatct
    67561 tcgatcgact ttttattttc gcctttctca ccaaaaagct tagcaacacc ggcttcaaat
    67621 aagtacccga tcgtacaaaa atgacctaaa gcaatatcat ttgatccagt gattttacga
    67681 atgatcggtt gcgctactgc cggcatagca atggcgaaac aagctccgat aaaaccaccg
    67741 accaagatca atacgatccc tcgcagaccg gcaaaatagc caaagactgt cgtcatcgtt
    67801 gccatccata aaattgcttg accagttaaa aaaatgtact tccattttgt gaaacgagcc
    67861 aaaataatat tgaatacaaa aatggctaag aacgtcagtg caatatctct acctaaaccc
    67921 aaatcattca ttgcttgacc attgatagat tcaatagaag ggatgatccc ttgcatatga
    67981 aatccttcag taaagatttt cccgaaataa gttaaacttc caacgatgat gctcgaaccg
    68041 gcactcaaca cttgaaatcc taggagtgtc tttaacgtgc cagagatgac ttgtcctgct
    68101 gatttcttct gcagtagtag acccaacata gcaatcaatg caatggtgat cgatgcctga
    68161 gtcaaaatat tttcgatgat aaaattgata acgcccatgt tttgatgtgc accccaaaag
    68221 ttagactaga aatctaatta aaaggggtgc tttttttgcg gaaatataca tttgttttta
    68281 agaaaaaagt agtttcagac tatttaaata acgaaggcgg ctacaaatat cttgcacata
    68341 aatatcaaat aaatcgtaca ctggttagac attgggtaag gatttataac tatcatggtt
    68401 gggaaggctt ggttggaggt ggcaaaagct acactacaaa atttaaactt gatgttatag
    68461 aatatatgga aacaaatggt ctttctatcc aagaaactgc taaaaaattt aatatcggtt
    68521 caaatagaac tctaagtaaa tggatagagc aatatgaaga aggcggtgct tcttcacttg
    68581 agagccaaaa aaggggcaga aaaattagta tgaattccaa gctaaacatt cctaaaaaac
    68641 ttaaagatga gtctcttgaa gaagaagtta ttcgtttaag agcagagaac gcatatttaa
    68701 aaaagttaga aaccttgatt caagaacaag atttgagcaa gaagaaatca aggttaaaac
    68761 aatccttcag ctcaaaaaag aatttaaatt gaatctatta ctttcaattg cacaattagc
    68821 gaagtcaact tattattact gggtaaaaaa attagataag ccagataaat atagcaagat
    68881 aaagcaagaa attacagcga ttgtaaaaga atcaagaaac tcttatggtt atcgtagagt
    68941 cactttagcg ttaaagatga aaggatatac aatcaatcat aaaacagtta gaaaattaat
    69001 gtcccaaatg ggacttacct gtcaaatccg aataaagcga tataaatctt ataaaggaac
    69061 agtaggaaaa attgccaaga atgtgttaaa acgaaatttt tcagtagata cacccaataa
    69121 aaaatgggtg acagacgtca ctgaattcaa gataaaagga agaaaaatct atctatctcc
    69181 tatacttgat ttatttaacg gagaaataat tagttatagt atatcaacta gtccaacata
    69241 taaactgatt gaagaaatgc ttcaacaagc aattaaaaaa aagggaactg agggttcact
    69301 gattctacat tcagatcaag gatggcagta tcagatgcca caatatcaaa aaaaattaaa
    69361 agagaataac attattcaaa gcatgtctag aaaaggaaat tgcttagata attctgtaat
    69421 agaaaatttt tttggtgtgc ttaaatcaga gtttttttat cgagaaaaat tccgatcaat
    69481 tgagatattt caaagtaaat taaatgagta tattaggtgg tataataaca aaaggataaa
    69541 actaaagtta aatggactat ctccagtaga atatcggaaa cagtccatta aatagtt
//
LOCUS       DAZUKC010000005        68203 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000005 DAZUKC010000000
VERSION     DAZUKC010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.005"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..420
                     /locus_tag="ACG80T_000274"
     CDS             <1..420
                     /locus_tag="ACG80T_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFS4839958.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(504..1226)
                     /locus_tag="ACG80T_000275"
     CDS             complement(504..1226)
                     /locus_tag="ACG80T_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFS4839959.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(1216..1953)
                     /locus_tag="ACG80T_000276"
     CDS             complement(1216..1953)
                     /locus_tag="ACG80T_000276"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4839960.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            complement(1965..2957)
                     /locus_tag="ACG80T_000277"
     CDS             complement(1965..2957)
                     /locus_tag="ACG80T_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4839961.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVNYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            complement(2972..3715)
                     /locus_tag="ACG80T_000278"
     CDS             complement(2972..3715)
                     /locus_tag="ACG80T_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4839962.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     regulatory      complement(3789..3893)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(3956..5278)
                     /locus_tag="ACG80T_000279"
     CDS             complement(3956..5278)
                     /locus_tag="ACG80T_000279"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS4839963.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     gene            complement(5265..6230)
                     /locus_tag="ACG80T_000280"
     CDS             complement(5265..6230)
                     /locus_tag="ACG80T_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4839964.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            complement(6227..7765)
                     /locus_tag="ACG80T_000281"
     CDS             complement(6227..7765)
                     /locus_tag="ACG80T_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFS4839965.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            complement(8181..8468)
                     /locus_tag="ACG80T_000282"
     CDS             complement(8181..8468)
                     /locus_tag="ACG80T_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4839966.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            8602..9270
                     /locus_tag="ACG80T_000283"
     CDS             8602..9270
                     /locus_tag="ACG80T_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4839967.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            complement(9561..10301)
                     /locus_tag="ACG80T_000284"
     CDS             complement(9561..10301)
                     /locus_tag="ACG80T_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4839968.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(10431..11378)
                     /locus_tag="ACG80T_000285"
     CDS             complement(10431..11378)
                     /locus_tag="ACG80T_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4839969.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(11507..12271)
                     /locus_tag="ACG80T_000286"
     CDS             complement(11507..12271)
                     /locus_tag="ACG80T_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS4839970.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            complement(12275..12664)
                     /locus_tag="ACG80T_000287"
     CDS             complement(12275..12664)
                     /locus_tag="ACG80T_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4839971.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            12799..13341
                     /locus_tag="ACG80T_000288"
     CDS             12799..13341
                     /locus_tag="ACG80T_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFS4839972.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13489..14121
                     /locus_tag="ACG80T_000289"
     CDS             13489..14121
                     /locus_tag="ACG80T_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4839973.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITVAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(14255..14380)
                     /locus_tag="ACG80T_000290"
     CDS             complement(14255..14380)
                     /locus_tag="ACG80T_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFS4839974.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(14514..15407)
                     /locus_tag="ACG80T_000291"
     CDS             complement(14514..15407)
                     /locus_tag="ACG80T_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFS4839975.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(15589..16515)
                     /locus_tag="ACG80T_000292"
     CDS             complement(15589..16515)
                     /locus_tag="ACG80T_000292"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFS4839976.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(16600..17361)
                     /gene="pflA"
                     /locus_tag="ACG80T_000293"
     CDS             complement(16600..17361)
                     /gene="pflA"
                     /locus_tag="ACG80T_000293"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFS4839977.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(17605..19836)
                     /gene="pflB"
                     /locus_tag="ACG80T_000294"
     CDS             complement(17605..19836)
                     /gene="pflB"
                     /locus_tag="ACG80T_000294"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFS4839978.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(20113..22563)
                     /gene="parC"
                     /locus_tag="ACG80T_000295"
     CDS             complement(20113..22563)
                     /gene="parC"
                     /locus_tag="ACG80T_000295"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFS4839979.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(22575..24620)
                     /gene="parE"
                     /locus_tag="ACG80T_000296"
     CDS             complement(22575..24620)
                     /gene="parE"
                     /locus_tag="ACG80T_000296"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFS4839980.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(24786..25220)
                     /locus_tag="ACG80T_000297"
     CDS             complement(24786..25220)
                     /locus_tag="ACG80T_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFS4839981.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            25347..25985
                     /gene="plsY"
                     /locus_tag="ACG80T_000298"
     CDS             25347..25985
                     /gene="plsY"
                     /locus_tag="ACG80T_000298"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFS4839982.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(26117..26992)
                     /locus_tag="ACG80T_000299"
     CDS             complement(26117..26992)
                     /locus_tag="ACG80T_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFS4839983.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVIRYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(27074..27865)
                     /gene="codY"
                     /locus_tag="ACG80T_000300"
     CDS             complement(27074..27865)
                     /gene="codY"
                     /locus_tag="ACG80T_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFS4839984.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(27883..29283)
                     /gene="hslU"
                     /locus_tag="ACG80T_000301"
     CDS             complement(27883..29283)
                     /gene="hslU"
                     /locus_tag="ACG80T_000301"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFS4839985.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(29297..29845)
                     /gene="hslV"
                     /locus_tag="ACG80T_000302"
     CDS             complement(29297..29845)
                     /gene="hslV"
                     /locus_tag="ACG80T_000302"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFS4839986.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(29882..30787)
                     /gene="xerC"
                     /locus_tag="ACG80T_000303"
     CDS             complement(29882..30787)
                     /gene="xerC"
                     /locus_tag="ACG80T_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFS4839987.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(30985..32361)
                     /gene="trmFO"
                     /locus_tag="ACG80T_000304"
     CDS             complement(30985..32361)
                     /gene="trmFO"
                     /locus_tag="ACG80T_000304"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFS4839988.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(32330..34408)
                     /gene="topA"
                     /locus_tag="ACG80T_000305"
     CDS             complement(32330..34408)
                     /gene="topA"
                     /locus_tag="ACG80T_000305"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFS4839989.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(34530..35387)
                     /gene="dprA"
                     /locus_tag="ACG80T_000306"
     CDS             complement(34530..35387)
                     /gene="dprA"
                     /locus_tag="ACG80T_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFS4839990.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(35443..36210)
                     /locus_tag="ACG80T_000307"
     CDS             complement(35443..36210)
                     /locus_tag="ACG80T_000307"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFS4839991.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(36203..37063)
                     /gene="ylqF"
                     /locus_tag="ACG80T_000308"
     CDS             complement(36203..37063)
                     /gene="ylqF"
                     /locus_tag="ACG80T_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFS4839992.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            37324..37722
                     /locus_tag="ACG80T_000309"
     CDS             37324..37722
                     /locus_tag="ACG80T_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4839993.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWVMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            37959..38501
                     /locus_tag="ACG80T_000310"
     CDS             37959..38501
                     /locus_tag="ACG80T_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFS4839994.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRVIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(38584..39135)
                     /gene="lepB"
                     /locus_tag="ACG80T_000311"
     CDS             complement(38584..39135)
                     /gene="lepB"
                     /locus_tag="ACG80T_000311"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS4839995.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(39287..39514)
                     /locus_tag="ACG80T_000312"
     CDS             complement(39287..39514)
                     /locus_tag="ACG80T_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFS4839996.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(39511..40029)
                     /gene="msrA"
                     /locus_tag="ACG80T_000313"
     CDS             complement(39511..40029)
                     /gene="msrA"
                     /locus_tag="ACG80T_000313"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS4839997.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(40049..40762)
                     /locus_tag="ACG80T_000314"
     CDS             complement(40049..40762)
                     /locus_tag="ACG80T_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFS4839998.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(40765..41661)
                     /locus_tag="ACG80T_000315"
     CDS             complement(40765..41661)
                     /locus_tag="ACG80T_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFS4839999.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(41819..42661)
                     /locus_tag="ACG80T_000316"
     CDS             complement(41819..42661)
                     /locus_tag="ACG80T_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS4840000.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEEILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            42793..43446
                     /locus_tag="ACG80T_000317"
     CDS             42793..43446
                     /locus_tag="ACG80T_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFS4840001.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(43510..44037)
                     /locus_tag="ACG80T_000318"
     CDS             complement(43510..44037)
                     /locus_tag="ACG80T_000318"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFS4840002.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEIDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(44057..45004)
                     /locus_tag="ACG80T_000319"
     CDS             complement(44057..45004)
                     /locus_tag="ACG80T_000319"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFS4840003.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKEVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(45016..46899)
                     /locus_tag="ACG80T_000320"
     CDS             complement(45016..46899)
                     /locus_tag="ACG80T_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS4840004.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSGDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            complement(47067..49139)
                     /locus_tag="ACG80T_000321"
     CDS             complement(47067..49139)
                     /locus_tag="ACG80T_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFS4840005.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRHADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            complement(49152..49427)
                     /locus_tag="ACG80T_000322"
     CDS             complement(49152..49427)
                     /locus_tag="ACG80T_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840006.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(49789..50244)
                     /locus_tag="ACG80T_000323"
     CDS             complement(49789..50244)
                     /locus_tag="ACG80T_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840007.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(50398..51606)
                     /locus_tag="ACG80T_000324"
     CDS             complement(50398..51606)
                     /locus_tag="ACG80T_000324"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFS4840008.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPSLKEEGEALLRFAELPSVPLKEESQVWTLLISQLGLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(51718..52050)
                     /locus_tag="ACG80T_000325"
     CDS             complement(51718..52050)
                     /locus_tag="ACG80T_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFS4840009.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            52198..53073
                     /locus_tag="ACG80T_000326"
     CDS             52198..53073
                     /locus_tag="ACG80T_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4840010.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(53155..53736)
                     /locus_tag="ACG80T_000327"
     CDS             complement(53155..53736)
                     /locus_tag="ACG80T_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFS4840011.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(53742..55001)
                     /locus_tag="ACG80T_000328"
     CDS             complement(53742..55001)
                     /locus_tag="ACG80T_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFS4840012.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIAPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(55003..56049)
                     /locus_tag="ACG80T_000329"
     CDS             complement(55003..56049)
                     /locus_tag="ACG80T_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840013.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQRPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(56284..56559)
                     /locus_tag="ACG80T_000330"
     CDS             complement(56284..56559)
                     /locus_tag="ACG80T_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFS4840014.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(56802..58112)
                     /gene="der"
                     /locus_tag="ACG80T_000331"
     CDS             complement(56802..58112)
                     /gene="der"
                     /locus_tag="ACG80T_000331"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFS4840015.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(58294..59523)
                     /gene="rpsA"
                     /locus_tag="ACG80T_000332"
     CDS             complement(58294..59523)
                     /gene="rpsA"
                     /locus_tag="ACG80T_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFS4840016.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(59658..60338)
                     /gene="cmk"
                     /locus_tag="ACG80T_000333"
     CDS             complement(59658..60338)
                     /gene="cmk"
                     /locus_tag="ACG80T_000333"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFS4840017.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAIQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(60386..61021)
                     /locus_tag="ACG80T_000334"
     CDS             complement(60386..61021)
                     /locus_tag="ACG80T_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFS4840018.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(61087..62490)
                     /locus_tag="ACG80T_000335"
     CDS             complement(61087..62490)
                     /locus_tag="ACG80T_000335"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFS4840019.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGFLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVIHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKHQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(62477..63508)
                     /locus_tag="ACG80T_000336"
     CDS             complement(62477..63508)
                     /locus_tag="ACG80T_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4840020.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYERILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            63539..63760
                     /locus_tag="ACG80T_000337"
     CDS             63539..63760
                     /locus_tag="ACG80T_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFS4840021.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(63925..64662)
                     /locus_tag="ACG80T_000338"
     CDS             complement(63925..64662)
                     /locus_tag="ACG80T_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4840022.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGNSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(64816..66156)
                     /locus_tag="ACG80T_000339"
     CDS             complement(64816..66156)
                     /locus_tag="ACG80T_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFS4840023.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(66393..67916)
                     /locus_tag="ACG80T_000340"
     CDS             complement(66393..67916)
                     /locus_tag="ACG80T_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFS4840024.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
BASE COUNT        18843 a        14342 c        11636 g        23382 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacaac ctgttgtaca agaaacggtt
      121 caaacggaaa aacaagcagc gcctgttgct gaaacacaac ctgctcctgc tgtaacagaa
      181 actgctgcta ctccagcaag cacaagctct gcaaaagaat ggatcgcaca aaaagaatca
      241 agcggttctt acacagcaac gaacggacgt tacatcggac gttaccaatt agattcttct
      301 tacttaaatg gtgactactc tgctgcaaac caagaaagag tagcagaaca atatgttact
      361 tcacgttatg gttcttggga agcggcgaaa gcattctggg aagcaaacgg ttggtactaa
      421 gattcattta ttaatcaaac agtttacttt tatacaaaga aagccaaaaa gaggtgcgac
      481 actagttgtg tcacatctct tttttattta cctaatctaa tgaaacgttc caagttttga
      541 taagtatttt ttttaacttc ttcaactgat agctctttga tctcagcgat tgtctttaac
      601 tgttcttcaa gcagtgagac ataatctttt tctgaaaaaa tcccccctaa agcccactct
      661 gctgcatgta gtccatccgt ttcaataagt aagtggtcga ttgggactgc tttagctagt
      721 tgccagactg ctggattctt tttcaaatca agaccaatgc tgaaataaca gcctaaatca
      781 atatagtctt gttgatagtc caaagaagaa taccaatgga tcaaatagtg attagggtaa
      841 gcttggatat aatttaagat ctctttttcg catccttttg tgtgcagtac aacaggtttc
      901 tcatttatgg ctgcaaaagc aagctgtcgc tcaaatactt ttttttgtgt agtcatcggt
      961 acattcgtcc aaatattatc taaccctatt tcaccgatga tccttgcttt tttcaaaaaa
     1021 ggctcaactt gttcaaacgt atagctatct gctttccaag gatggatacc gtaacttaag
     1081 gcttgagtct tacttgctgc aagctgtctg ttttccttcc attcttctgg cgaatcacaa
     1141 ttgatgatcg tcaaaatcgt gtgttcctgc tgaaggagtg ctaaactctg actcccatct
     1201 aaatgacaat gagcatcact taacatctaa ttcacctgcc aataactgat aaatcgtttg
     1261 cttcaactct gattgctgca gtagccattc acgtgaccgt cgtttttcag gcacttgaat
     1321 ctcagcaatc acatgtgccg gacgggcgga aagcacaaga atcctgtctg ataaataaat
     1381 tgcttcttcg atatcatgcg tcacaagtaa agtagtccgc tttgcttttt taggcagctt
     1441 caacatccag tcttgcatct gccctctggt catagcatct aacgctccaa atggttcatc
     1501 aagaagcaag taagaagaag gattcatgat tgttcgtaac aaggccgttc gctgcttcat
     1561 cccgccagat aattgttccg gcaaaaaatg ttcatagccg tttaacccaa agatatccag
     1621 ataatccctt acttgttctt cattgattgt tagatgattg attttttgat ataacgtaac
     1681 gttttgatag acgctcagcc aaggaagtaa catatcttct tgcggcatat aactaaatgc
     1741 tgtatggaga ccagttactt tttgatcgtc tactttgatc gttccagaag tcataggctg
     1801 gactcctgtt aaagttttca gcaatgttga cttaccggct ccgcttggcg cgacaatcga
     1861 tgtaaaggta tctttaggga tagtgaaatg gatgtcttct aatattttca ggttttcaat
     1921 gactacattc aagtggctga tttggattga catatctatc actcctattt tataaactga
     1981 ttagtgaata gttcttgcga aggtacatct tgcttgatca atccgttttc ctgcataaaa
     2041 gctgtataat tgcgccaaac tttttcttcc atcaatcccc aattctcagg atcatccgta
     2101 taattttttg acaagaatgc ttgtgattct tctaaaaacg tccgatcatt ttcttttgca
     2161 tatttttgca acagtttagc actgtcattt ggatccttaa tagcttcctg atagcctttt
     2221 ttcgtggcat ccataaaaga ctgtaccaat tctggatcac ttttgatcag ttgatcattc
     2281 gtaataatta ccggagtata gtaatctaac cgttcatcta attctttcaa tgggatatag
     2341 tttagttcaa tacccgcttc ttttgctttg atcatatccc aaccttcaaa gtaccattgg
     2401 atatctgagg attttcctaa gctttctggc ccttctccat tgcttcctac cattgtcagt
     2461 tttgaaaagt ccccgccgtc tttttccatc acagctttta agacagcttc ttcacttgga
     2521 ctttgccaac ctgcatatgt ttttccttca aaatcttttg gtgaatggat gttactgtct
     2581 gatagggtgg cgaatccaga agtattatgt ttgatcactg tagcaattgc tttgactggt
     2641 atattctgtc catctgcgtg agcataagtg acatcttctt gataatttac accaaattgt
     2701 gctttgtccg cacctactag accaatactt gtcgaattat ctcctggttc gatgatttgg
     2761 acatttaaac cagcctcttt ataatagcct ttttcttttg ctaggtagat gccagtatga
     2821 ttagtattcg gtacataatc caaaacaagt gtcacttttt tcaaatcctt tatttcttgt
     2881 ttgctacttg tttcttttgc agtgccacaa gcggataaaa gcaacagtag cggtaataaa
     2941 agccataatt ttttcatttg atccccttct cttaaaaaat ataaagcata cgataatacc
     3001 cttttttcaa taatgaacaa gcgccattca gcaataaact caatagaatg acacaaatga
     3061 tcgcagcaaa aactttatcg atctggtagc tgttttttac gcgtatcatg taatacccta
     3121 agcctgcttg agcgcttagc cattctccaa cgactgctcc gctgacacaa taagtagcag
     3181 ccacttttaa accggaaaag aagccggata aacccgctgg tattttatat attcgataaa
     3241 tcttccctgt atctgccccc atcgttttca aaaagatgat ttgctctttt gatactttcc
     3301 tcaatgtatc cgaaaaagaa actacgatcg gaaaatagct tatcaagaca acaaggatca
     3361 cttttggaag cataccgaaa ccaaaccata gggtaagtaa tggccctaac accatgattg
     3421 gcaacatttg tgaaatcact agtaacggat aaaaagacat ttctaaaaac ttccaataat
     3481 ccattgccaa ggcagtaata aaagaaagaa cgctagctat taccaaacca agcactgctt
     3541 catttaatgt cgtgacagaa tgggaaagta atacttgtgc atctgccgct aatgcagaaa
     3601 atacagcagc aggtccgggt aaaataaagg caggtatttt ccaaactatg acaacaatct
     3661 gccaaataat taaaatgaat gccatactga ctgctggata aagatttttc ttcattttgt
     3721 tacctccttt cttctaatat gaataacaaa aaacgcttga aatgggcacg acatagtaac
     3781 ccgcttcaag cgtctgctat gtttctccct tcaccagtat taactgacag gttcaatggg
     3841 tctaagttca caacttactc tcatgtcgtc tacgacatcc ccagaaacag gtattcattt
     3901 tttaaagtgt agcacaagaa ggaaaaagca acaagcgctt tagttatcat gctgattatt
     3961 tcaataaata ggagagatca cttgccaatg tcggataccc catgatatag ctatccaact
     4021 cttttttagt caatttttga ttgatgacga gtgtcaaatc atcgatcaat tcgtctgctt
     4081 gctcgctaat cactgcagca ccaatcaaat aattttgctg gtcaaaggtc aattcagcct
     4141 tagcaactgg ttcattgaca cgatgatagg tgaaccaact ggtaagatcc atctctacca
     4201 cttgatctga cgaagaggcg tgactttttg taactcctac ttcagcaagt ttaggactgg
     4261 cgtagacgat cgtaggtata atcggatatt tgataggttc gcttgttgca tccgttatcc
     4321 gcttagctac ataatttcct tcaaaagtgg cgactggtgt caattttggg gttttccttg
     4381 aaacaatatc tccacacgca aaaatttttg gattgctcgt ttgcagataa tcattgactg
     4441 caattccgtg tttatcaaag acgatattcg cttgctcaag tgctaaagat tctgtattcg
     4501 gctgtctccc agtcgcacaa aaaatcatat ctgctactaa ttccgtctct tttccgacta
     4561 aacgatagcg agtaccttct gaaatgatct tttgggtttc aacaccaaaa gcaaactgaa
     4621 tgccagatgc ttccatttga tggacggctt cctctaccaa agacgcctca aattctttta
     4681 atggccgttg attatgatgg acaatggtta ctttgctccc tgcagcattg gcaatcgtag
     4741 ctagctcaaa agcaatataa ccaccaccga taaaaatgat ttctttaggc agtacaggca
     4801 atgataaaaa gtctgcacta gttttcaagt attcctttcc ctccactggt aagatcgtag
     4861 gtctttgccc ggttgccaaa actaagtaat ccgcatggaa gacttcttcg ttgacttcaa
     4921 tagatgaatc atccaaaaaa cgagcggttc ctgaaagatg atcgatttca gcttctgcta
     4981 attgtttttt tcggctttct gggactggat ccgtaaatgt tcgtttgaat ttttgaagtt
     5041 cttcccagtt ggcagttggg atctcgttaa aaccttttcc actcaattgt tttacccgat
     5101 tacgtgcctc tactgcactt aacaagactt ttttaggatc acatccacgg ttaggacaag
     5161 tcccgcccca tagatcttct tcgaccacta gtactgtttt acctgcttct ttcaatccat
     5221 aagcagcact taagccgctc acacctgaac cgatgatgat tgcatcatat gttttcattt
     5281 ccttcctcct ctcgacttaa aaaagcaaag atttctttac ttgtcagcga cccaatactt
     5341 ctttgctttt ctactatcac aatgcttgga tacaccgaaa gaagcgaata tacttctttc
     5401 ataggcgtat caaagcaaac ttgttgacga gcattttctg gtatttcatt taagaaatat
     5461 ccattgacga ctaaatcttc tactcgccac tctttcgtct gtttcggttc atcaaagaaa
     5521 tgccgaacgt agtcgttttt aggattcatc aataactgct ctggtgtgtc acattggata
     5581 atttctccat ccttcataaa tgcaatacga tcacctagct tgattgcttc ttccatattg
     5641 tgcgtaacga aaacgatggt cgtgcccaat tcttcgtgta aggacaaaac caattcttgt
     5701 aatgagtttc ttgaaatcgg atctaatgcg ctaaatggct cgtccatcaa aatcacatct
     5761 gggcttgcag caatagctcg gatgatcccg atccgttgct gttctcctcc agacaactct
     5821 ctaggcatac gatctcgata aatatcagga gataagccaa ctttttgaag aagttcatct
     5881 acacggtctg ctcttttctg cttttcccaa cctaacattt cagggatgac ttcaatattt
     5941 tgcttgactg tcatggtcgg gaacaaagcg atttgctgca gcacatatcc catattccac
     6001 cgcattttct gcagattata ttcatttaac gattttcctt tgaagtagat gtctcctgct
     6061 gatggtttag ataatccatt gatcattttc aaagtagttg tttttccact accggaagga
     6121 ccaaccaaaa caaagatttc tccttttttg attgccagat ttaggtcatc gatgacatac
     6181 tttccatcat actttttttg cacatgtttg aactcgatta atttagtcac tgatcttcct
     6241 cctttactaa tccttcattt ttcaggaaga ttcgtgctac ttctgtcggc tttttctttt
     6301 cgacattgac ttggtaattc atatcaatca tttgatcctc tgttattttt ccgcttaatt
     6361 tattcagtgc tttaacgatt tcaggatgtt ctttagcaaa atcttttttc atcagcggtg
     6421 cgccttgata agctgggaat aaccctcgat catcttctag agtcactaga tcgtactcct
     6481 tgagctggct atctgtggaa taggcatcca caatattgac atctccatta ttgattgctt
     6541 ggtaacgcaa acttggctcc atgctttgta cacttgaaaa attcaagcca tatgcttctt
     6601 ggattccttt atatccatct gaacgatcaa taaattccag tgtgaatccc gctttgatct
     6661 gatcagagat agctgccaaa tcggaaatct ttttaagttg atgttcgttc gcaaaagatc
     6721 tcttgactgc caaggcatac gtattttggt aagccattgg ttttaataat gtcatatcga
     6781 attgcttatt caataattct tctgcttcgt catatgtttt ttcttttgtc aaatcagcac
     6841 ttttcattgc ttcaggtacc ttcaccaagc tttctaggac agtacctgtg aactcaggat
     6901 agatatcgat ttgaccttga tccagtgcat tgaacaaaaa actcgtcttg ccaaaattcg
     6961 gttttaattc aacttctgtt tttgtgttgt cttcaatcaa ttctttatac atttggatca
     7021 ggatttcagg ttccgctcct aattttccag caatcgtgat ggtttcattt ttaaacaagg
     7081 cagactgata taaagaaacg ccgccaaata aacacaccac tgctgctaaa gtaatgatgg
     7141 aatatcgagg tttcttatgc tgcaaccagc gaatcagtcc gctgaatata attgcaagca
     7201 aggcagaact gatcgcacca atcaatgtca aaacaggtgt atttcggtca atacctaaca
     7261 aaataaatgt ccctaatcca cctgctccga tcaatgctgc caaagtagcc gtaccgatga
     7321 ttaaaactaa ggcagttcgg atacctgaga taatgacagg taatgcgatg ggaagctcta
     7381 ctttcagcaa gcgtctcatt ctggacatac caaatgctac cgctgcttct tcaatagacg
     7441 ggtcaatttc agctagacca atatacgtgt tttggaaaat cggaagcaaa gcgtaaataa
     7501 caagtgcaat caatgctgga acagttccga tccctacaaa tgggataagt aaacctaata
     7561 aggcaagtga aggaatcgtt tggagcacac ccgtgatctg caataagact tctgccattt
     7621 ttttatgatt ggctgcccaa atcgctaaag gaatagcaat cagtgctgca atcagcaaag
     7681 aaatgagaga aatggacaga tgttgaaatg cagcttccag caactcgcct tttctttcac
     7741 ttaatgtttg tataaaatta ttcatgcgac cttcctttct gttattcttt aagtataaac
     7801 atttctttcc tttttcccaa ctaacttgtc ttgccttaac cttacagcac gaacaaaaaa
     7861 aagaaaaaaa tacctctaaa tatgctgaca aaaaaagaat ccaagcaaca aagtcggatc
     7921 aactttttgg ttctcgtttt ttcactacga ataccataaa aatctatacg aaattgtcac
     7981 ttggaatcag ctatatcttt tttacacaca caacttttat tttaataaaa agaaacagcc
     8041 aataaagtag aaatctcgtg ggggagaaca cctttattgg cctgttcaac gaatattata
     8101 ctgtatcatg acactttttt gccaaaaatg taaaaaatgg ttgttttgac atcataaacg
     8161 tcttgttttc ttatttttac ttatatcacc actttatttt ctatctcttt cactccataa
     8221 agaggaatga gttcttcttt tgactgataa taaacagctt tctcacggtt cttcatcaaa
     8281 aaaagaaaga tgtctttcat agcaagtggt gctttgccgt gttcaaataa aaagataaat
     8341 tgatttgctt tatttatctg gaccattgtc acaggcacca gtttttgttc ttgagccaaa
     8401 aaaactcctt gatactctgt ttttttgggt gattttgtaa gaattgtcaa taagtctgct
     8461 gctcgcattt ttccactcct tatttgataa ataaaaagaa acagccaaaa ataatttttt
     8521 ttcattttcc tcttgaagta agagtataag tcagtataat aaagaagttg ctgaaaattt
     8581 tcaaaagaaa agaggctaga aatgaaaaaa ttaaatttat ttacagaatt gtgcggaatc
     8641 attttaggcg caggaatcta cggattagct gtgacaggaa tcaatattcc atctaagctg
     8701 gcagatggtg gggttactgg tattgctttg cttttgcata atctttttgg attcgctcct
     8761 tctgtcactt cactgctttt taatttacct ttattgctta tctcattgtt tctttttgga
     8821 aaacacgctt ttctccggac gatcgtcgga acatttgcgc tagttttctt tttacattta
     8881 tgggagagcc ttcatataca ttttgctgta gatagtctac tgatcaatag tttgatgaca
     8941 ggtattcttt ctggcattgg ttgcggtctc gtctttcgat tcggcggaag cacaggcgga
     9001 acagatatca tttatcaagc cattgaaaaa tattttcata ttaagattgg caaaagtctt
     9061 ttcttgatta cttttggtat tttagttgtc tccttgcttt atttggatct cacacatttt
     9121 ctgtatacat tacttagctg ttctattcta tcttatacat tgaataaagt gaagtatctg
     9181 gaaattggat ctgttttgcc ccaattggaa gcaaaacaga ctacagaaac atttgaacca
     9241 cttgatcctc ttgaacagtt agagcactga ttcttatttt tcactacttt atttaattta
     9301 cagaaatatc attatgtaaa ctaaaatgat attattcagt aaaatcttct ttataaaaaa
     9361 ataaactatt tttgcaaacg atctgcgaaa atagtttgtt tttttatttt gccattattt
     9421 tcttacttat tgggagaaaa aattcgctta aactcaaaaa aatttgagga tacgatataa
     9481 actttcggat agagtataaa ataaggaaac gccgcagcgt ttcctttcca actgtagccg
     9541 aatataaatt tgtatatcac ttatttaaaa tagctatttt ctcccaaaga gtttccttga
     9601 tcatcgatca atccttgtaa tgagcgttct aagataccat ggcttttttt cgattttttt
     9661 tcttctgact gctttgtttc ctctaacgtt tttgttcgat tgaatgttcg tacagacggc
     9721 tcaccatttc ttttttcttg atacggcgtc ggttctgtat cgaacaacag ataagagtca
     9781 tccgtttttt tcatcgattc gattaaatga tcctcagaaa ttttgctttc tctagggtct
     9841 gggataatcg atgcgggtac atatttagga acaaaataag aacgctcccg caccttcgtg
     9901 tcaacagatg cgtttcgtgt agtatgcttg gactctgtcc ttgtttcact ttgtctgaca
     9961 gaacgagagg aatcaccaaa aagttttttt cgtcctgtcg atgtgatttc tgattggtaa
    10021 tggccggtat aatccggtaa ttttccttta tgcttttcca aattttcctt acgcggagct
    10081 gacttcaatc catttgttgc acgacgcttg cgacggattt tctcggtgtt cgtgttcgtg
    10141 aaatgaaatt ctttcatctg ttcaaacggg gactgattct gttcagtcaa aagatcttct
    10201 tgtccgtcaa ataatactcg cttcttgtcg ttggttaatt ttacgcctaa ctcatcatcg
    10261 tacgcaggaa aacggaaatg ttttcgtctg atttctttca ttcaaaccat ccttctcaaa
    10321 aatggaactt ttctcaataa tagcacagat tcattgtttt cagtagcaat gcgctgatta
    10381 ttttaagaaa atagcgatga atttcataaa tatatatata gtaataagac ttaattcgtt
    10441 gtctctctga taactagttc aggaaaaaaa cgacgataac cttgactcgc ccctttttct
    10501 tctatttttt ttaacaacat ttctccacat acttcaccca tacgtataat atcttgcttg
    10561 atcgttgtca atttaggtga actgatttgg tctaaaaata ccccatcaaa tccaatcaca
    10621 ccaaattctt cgggcaagtg tcctccagca tttataatcc ctcttactgc tcctagtgct
    10681 agccgatctg aactacatac aaaacaagtt ttatctgtaa aatctgccca gttttctgtc
    10741 atatatcttt ctacttctgt cgaatgattt ggaaaccgca agattttact aggaagatca
    10801 acttccttca tcacagataa ataaccagtt tcccttgaaa tttcaaaagg ctcgtccact
    10861 tcgataccga tataaataat cgtttgataa tctctttcta aggcatattg cgttgctttt
    10921 gctgtaccat attcattatc gctgtctaca taatcatatc cttcagaatt ttccccaaac
    10981 aagacaactg gtttttccaa acggtcgatc caatcgaagt ctttttttct gacacctgtg
    11041 atgatgtatc catcacaaga accgacgtcg aaggcatcat ttgtaactaa ttggagagaa
    11101 taatgcgctt taccgatccg tttagcgatt cccatcagca aattcatgta atagggctca
    11161 gttgtgtcga tttcttccag tataaataat ttgatgatct gcgaccgatt cgtgacaagt
    11221 gcttttgcag caatatttgg acggtaatcc agttcagcca ttgctgagaa aactaactct
    11281 ttcaattcgt cagtcacctg ttccggatga ttgatgactc gagagaccgt catttttgaa
    11341 acattggcac gttttgcaac atctgctaaa gtgggcatgc tctcttcctt tctaacatgt
    11401 tgtcattctt actatcaatt ataccaaatt ttcctaacag agtttattat agcccaaaat
    11461 aaaaaacgga aaatcctcta tcgtcatgag tgattttccg tttcaattat ttatactttt
    11521 ttatcaaatt ttttgctaaa aaagtggaag cttcttccat tagtggtatt tgtaccatat
    11581 gtcgctgtgt ttttgaggaa cgaaaaacaa tttcttttcc gtaagactta tgccgatttt
    11641 tttctacaaa tcgttccgtt tgtatataag ggattttttc gtcagcttgt ccatgccaaa
    11701 agaacagagg acgtccagct aacttttccg gctgcaatga caaatcatag ctcttaaccc
    11761 atgaaatcaa atcaaaatag tcttttggta cgacataatt atattccgat gcgcgcctta
    11821 cgatttcttt tccatattcc aaaggtgcag gggaacccat caaacacacg cccgctacta
    11881 tatgaggatg tttagccaac aaggcgcatg ttgtcattcc ccccatcgaa accccgccaa
    11941 ctgcaagctg atcatgtaga agacctcttt tttctaaatg cttgataagt gtgtcaaatt
    12001 caaacagatt tccgtagata ctatcccaaa atgtatagga tggaatagaa gaaaccggct
    12061 gtttgcgctc cccatgattc atcgcatctg gcaggatcac acgaatgccc tgctgcgcaa
    12121 tttttcttcc ttgcgtcaaa actaactcct tacttgtctg ccatccgtga taatagatga
    12181 caacgggaat acgttcctct cttaattctt tcagaacaac ttctaacact ggaatatgat
    12241 ggacagaacg tttcaatata attggattca attgttactc acctattttc attaaatttc
    12301 ctgatcgcca tagcaaccat gctccaacag ttgtctggaa caggttcagt gtaccaaata
    12361 atataaatgc aggactgaat ggctcgggaa ataagcacag agaacaaagt cccaccactc
    12421 ctaatgctag cgaaaggagt ggataaatag aaaaattcaa aaaactggca acgctaccga
    12481 aaatattacc aataaacagt atcaaaaaga aaaaaacgct taaaattaaa ccatctgtca
    12541 gcgacatctg aaaaacaatg ttctctacct caattggctg ttgaatcgaa tagacaccta
    12601 agtatatact gagaaggaga cttagcacgt ggctggcaca aaacacacga ttgaccgttt
    12661 tcattttaat cacctaacct atctttataa ttctattata atcattttat catttttttg
    12721 tatgtataaa gtttcaattt ttcttcactt acgatttttt ttgatattgt tgttatataa
    12781 atgaaaggag ttttacaaat gattgatcca aaaatgttgg ggatgatctt cctcattaat
    12841 tttgcttata tcactctgaa cacgttacgt ttcatgttga caatgaaagg ctatcgactg
    12901 attgctccac ttgtcagtat ggtcgagatc acgatttata tcgtcggctt atcccttgtt
    12961 ttagatcgtt tagataatcc actgaattta tttgtctatg cgttaggtta cgcagtaggt
    13021 atcagtgtcg gaattttgat tgaagacaaa ttggcattag gatacatcat ggtcacaacg
    13081 attttgccag caaatagcgc agaagataaa agtttgccgg gtatactgag agaacacggt
    13141 tatggtgtga cacagacttc tgcaatgggg ctggaaggtg agcggctagt tctcgaaata
    13201 ctttctccta gaaaaagcga acgtgaactg tatcgcctga tcaaagaaat cgaagagcgt
    13261 gcttttatca tttcttatga gccaaaatat atttcaggtg gtttctggac taaaaaagtg
    13321 agaaaacgac aaggaaaata actcacaaac ttaaaaactg tctaatatct aaattaaatt
    13381 ttaaatgtta gacagttttt atttattatt ttgattaaaa aacatcgaca tatactcatc
    13441 aatttgttat tatactttat atacttatca atttaatttg gagatattat ggaaattcaa
    13501 cgtagaacca ctaatttttt acatttaggc tctttttttg caactttgac gatcttattt
    13561 tttctatgtt attcatacct taagacctca catgcgcctt ctagtgcatt aggctggttg
    13621 cttttggatc attctgatat cacagtagcc gaagaaaatc cgtttttttt gattttgtct
    13681 tctctttgcg gcttactgtt ttctgtttat ttcggtgcct atttttttca tcgacatcct
    13741 ggtacgttaa acgaagcttc aagcgtattg attgcattta aaacgttttc ttttttagca
    13801 attctttttt attcgtttaa tcacaatacg cttttcttct ttatcatgaa tggtttagtc
    13861 gtgattttat ccatttttac ttattttatt ttttcactca tttttagcca attgaaatgt
    13921 ccgctcttct ttcgattagt tcctgtcacc tgttttattt acagcttgtt ttcctttttt
    13981 gtccttgctt taatgccact gaccaaccct tcgacgatcc aagtcgcagg aaatctgttg
    14041 gatatgctct tcatcgtcag tctaggtgta tttatgtggc aaagaaaaaa caaacaaaat
    14101 aaaaagcatt ttgaagcctg actattacga cttcataaat gcttttttag ttttaaacac
    14161 ttcaatctaa aagaataacc caagattgga taaattatta atgactcgcc attcgtttct
    14221 tagtgattct ggattatcaa atggacttcc taatctaatc agctagttct tcccataaaa
    14281 tgccatactt gactgaatca taatattgat tatttaaaaa tctaactttt cttatcacta
    14341 cttcctttgt cattccacat ttctcaccat ttttttgcat tcctttgttt ccagaccatg
    14401 tagtgaatct aataggtggt agataggcaa aatactcaaa taattctttt aaccataaag
    14461 gaagagaaag gctcctcctg caagagtcag tataattttt gcttactgct ctcttaggtt
    14521 ttactcacta aaactgttcc atattctttg atataagaaa atgcatgcgg tataaatttc
    14581 ttgctcagct cagaaaagat ttgtcgttta agaaaggcca tttcctccat tgtcaatgaa
    14641 ggatccaatg ttggtaattc aatctcaaaa ttgttttttt cgacaatagg taattctaat
    14701 gtgaatgatt cttcttcctt tatagtattc gtcacaagta gagactgatg gaaaatacta
    14761 ggccggcgat ttttatattt attcttacgt atcctgtcaa cgataacttc tatttgatca
    14821 aaaaagtacg attcatttac gtctccaata agttcggcta attctttgaa tgccttcgtt
    14881 acaggaagct gattcttctg tacaaaaaag taattagcca aagtaagcgg gaagtgttct
    14941 atagaggaaa gtgtgataaa acggtacttc tcatttcctg cagttgtcct cagcaattct
    15001 gtctctcggc tgtttcttac tccataaaaa tacgcttctt tccgttcatc ttcgaaacaa
    15061 tacgcccaaa taacttctcc catcgctaat ttttgttcat caacagagag tctcttcaat
    15121 tgttcagcta tggtttcagc agccgaagtg gcttgttgca agtaatcttt aggatcaaat
    15181 attggaaagt tcaaatgata tcttcgttct tccctcttga ttaatcctgc atcgatcaat
    15241 aaatcaagaa aatgggaaaa tttattttgc gaaaattctt ttttcaattc tcttagaatc
    15301 gtttcttgtt ctcgatgttc atatagataa tcggtcaatg aagaaaaaag cggatcatcg
    15361 gtcagttttt gataaaagtt ttccttattt tttgaataat agttcatata tgttctcttt
    15421 tctatccgtt ttattttact taaaaggctt ttacgtaaaa aggaggaaga acaagagtca
    15481 taattgactg ttattctcct ccatttcttc ggcctttagt atgttctatg tttcttttta
    15541 tatcaatcgc ttatccattc attttgaatg cttcatacta ccatttgatt agctaaatac
    15601 agccgtcaat tgaggtacga cttgtttttt acgtgaaacg acacctttaa gcaaagcacg
    15661 gttgttggtc aaagttgttt ggaaagcttc ttctactttt tcttttcctt taccagcaac
    15721 aagtaattct gaatcactgt ctaagatgtt tgtgacaact aatacgaaca gatcgtagcc
    15781 atttgctttc atttcatctt ccattgctgc ttctagctct gcttgtcgat ccatcacttc
    15841 attaaggtcc actgtattga cttgagcgat acgaacgttg gaactgccca ttgggaaact
    15901 ttttgcatcc aaatcaagaa gaaccgcagc tgatttatca cttagatttg ttcctgcttt
    15961 caacatttca agtccataag tgtttagatc gacttcagcg attgctgcaa gttcttttgc
    16021 agctgcttca tcttcaggcg tacatgttgg tgatttgaac aacaatgtat ctgaaatgat
    16081 agctgagagc atgattcctg cgatttcttt aggaattgtt acttggtttt ctttatatag
    16141 ttttaaaata atcgtacttg tacatccaac tggttctgca cgatagtaca atggattagc
    16201 tgtttcaaag tttgcaatac gatggtggtc tactacagct aaaatattta cttcgctgat
    16261 atctgaaata ctttgttgga attcattatg atccacaagc atgacatctg cattttctgc
    16321 tttagctgac tctactactc gtggtgcaga tagctgaaag tggtctaatg cgtattgtgt
    16381 ttcttcatta ggtgtaccta gagcaactgc ttctgtttct ttacctaatt cattttgcaa
    16441 gtgtgaaaac gcaatggctg ccccgattgc atctgtatct ggattttggt gaccaaaaac
    16501 taaaattttt gacatgtcat tcgctccttt gtgatttctc ctatttaata atagcaaaaa
    16561 aacggaaaga agcaatactt atcttctttc cgatgcgacc tatcgcgtta aatattttgt
    16621 atactcttct gtatgaagca atcgtttagc attttcgaca cgttcttttg taggtggttc
    16681 gatcccttct aatggatatt tcaaaccaag tgtttcccac ttgtattttc ccatcgtatg
    16741 ataaggaaga atctctactc tgtctacatt tgacaaggtt tgaataaagt tgtctagtcg
    16801 gatcaaatat tcatcataat cacttcgctc aggaactagt acatggcgaa tccaaaccgg
    16861 tttgttgatt tttgacaaat attgagccat ttctaaaata ttttcattgt cccaatgcgt
    16921 tagtttttta tgctcttcat tatcgatgtg tttgatgtca aataacagta aatctgtata
    16981 cttcatcaat tcttgaaaac gtgaaaagaa aggttcttca tacgtaaaag gttttccgca
    17041 tgtatccaat gtagtatgca tcttccgctc ttttgctttt ttaaatagtt cgatcaaaaa
    17101 atcgatctgc aatagaggtt ctccaccact aacagtaata ccacctttat ctccccagta
    17161 aggtctgaag cgttctgctt tatctaaaag ttcatctgct gtgtattctt tccctccgcc
    17221 aatattccaa gtatctggat tgtgacaaaa ttggcaacgc atgcggcatc cttgcataaa
    17281 cacgacaaag cgcagtccag gtccgtctac tgaaccgaaa gtttcgatag aatgaacata
    17341 gccgattgtt ttttcttcca tttaagttca catcttttct ttgatttcat tttaagtaca
    17401 agttagctgt cgaagcgaag ctttctgaac aaaatttttc gcaattgccg cttcgttatg
    17461 tgtaaaattt acagggacgg aacatacaag tcctgcccct gtaaatcgtt tctttcaaat
    17521 aagcatactg attattcgat tttgatgcga tggctattca acgtcctttc gacgctaagt
    17581 cgaaaaactt ttttcacgaa ctttttacat tctgtcgtgt gaagttctca tcaagacatc
    17641 taattgttgt tctcttgtca agtcacggaa tttaactgca taaccagata cccggatcgt
    17701 caagttcgga tatttcattg gattttccat tgcatcacgc aataaatcat tactgaatac
    17761 attgacattc aaatgataag ctccttttga gaagtatcca tctaagacat tacgcaagtt
    17821 ttcgattctt gtatcttgtt cttttccaag acctgaagga ttgattgttt gggtatttga
    17881 aattccatcc aatgcacagc cgtaattcat tttagctgtt gagttcaggc tagctaataa
    17941 cccactttgt tccgcattgt aacttggatt tgctcctggc gcaagtggtt ttccagcttc
    18001 acggccatca ggagtatttc ctgtattttt accgtaaaca acatttgaag taatcgtcaa
    18061 taatgaagtt gttggttttg agttgcggta agttttatga cgtttgattt gtcccatgaa
    18121 gtattccaag atccatgaaa ctaattcatc tgcacggtca tcatcatttc cgtatttagg
    18181 gaagtcgcca tccacttcaa aatcaactgc tattcctttt tcattacgga ttgcttttac
    18241 ttttgcatat ttgatcgctg acaagctgtc tgccgcatga gagatcccag caatacctgt
    18301 agcaaatgtg cgttgcaagt cagaatccat caaagccagc tgagctgctt cataagaata
    18361 tttatcatgc atataatgaa tgatgttcaa cgtattgaca tatagttctg ctaaccaatc
    18421 catcatattc atgaagttat gtttcacttc ttcaaagtct agtatttctg atgtgattgg
    18481 acgatattct ggtccaactt gggcgccagt cacttcatcg actccgccat tgatggcata
    18541 taatagtgtt ttagccaagt tagcacgagc gccaaagaat tgcatgtctt ttccgacaac
    18601 tgttcctgat acacagcaag caatcgcaca atcatcagaa ccccattgtt cacgaagctg
    18661 attatcattt tcgaattgaa tcgaagaact ttcgatagcg attctgctag catatgttct
    18721 gaatccttct ggaaggtgtt cagaatacaa aacagtcaag tttggttctg gtgaaggtcc
    18781 catatttgtt aatgtatgaa gcagacggaa atcattcttt gtaactaatg aacgaccatc
    18841 taatcccata cctgcaatgg ataaagtagc ccagataggg aatcctgaga ataattggtt
    18901 atattcaggt gtacgcgcga atttgaccat acgtaatttc attactaagt ggtcgatcaa
    18961 ttcttgtgct tctttttctg tgattactcc attatccaaa tcacgttgga tatagatatc
    19021 aaggaatgct gagatacgtc cgattgacat cgcagcacca ttttgtgatt tgattgctgc
    19081 taaatatccg aagtatagcc attggacagc ttcttttgca tttgcagctg gtttagaaat
    19141 atcatagcca tatttcgcag ccatttcttt catttgcgct aatgcacgat attgttcagt
    19201 gatttcttca cgtaaacgaa tcacttcttc actcattgta tgataaccag tcatatcatg
    19261 atcttttttc ttttgttcca tcaaatagtc gattccatac aatgccacac gacgatagtc
    19321 accgatgata cgtccgcgac cgtaagcgtc tggaagccct gtaatgattt tgtttttacg
    19381 agcagcacgc atttcagcag tataagcgtc aaatacacct tgattatgtg ttttgcgcca
    19441 gtcagtgaaa atatgtgtca attcatcgtt tggtgtatag ccattgcttt ttagcgcatt
    19501 gttagccata ttgattccgc cgaacggcat gaatgcttgt ttcaaaggtt tgtctgtctg
    19561 tagaccgacg attttttcca aatctttatc caaatagcct gcatcgtgtg aagtaatagt
    19621 tgatacgatt tctgtgtcca tatcatagac accgttacgc tcgaattgga tatcatttaa
    19681 cttttgcaat tcatcccata atttttctgt tgcttctgtt gggccttcca aaaagctgtc
    19741 atcaccgcga tattcagtgt agttattttg aataaagtct ctagtgtcta cgccttcttt
    19801 ccatttggta cctttaaatc cattccattg ctccattgta tggacctcct aaatttctgt
    19861 tttatgtaac agtttttacg tgattagtat aacacagcac cctatctttg caagggtttt
    19921 cttgtttttt atttaacaat ttttatgttt tatttacata tatttaaatc aacgttgaca
    19981 taccaacatt taagcattgt gaacactttc atcttttttt gcaaacgtat aaggatttat
    20041 taaaaatgaa tgaaaaagag acgccacctg tattaataca gatggcgtct cttcattaaa
    20101 atactacaac agttacgact cgatgaaaat ctcaggagca tcgataactt cgatgacttc
    20161 tccacctttt ttctcatcca tcgcaaatga tccattcgat gttcgatcac ctatagggaa
    20221 atcagttggg tcaatcgtta actgctgccc tttttgattc atcaaggtta tttcttttga
    20281 tcttccttcc gacatataga taatacggtg cggatttttt ttcagctcac gcatgaccgt
    20341 cactccccgc ttcgcacgtg aagtttggct gatttcttga gcaagcatgc gtttaacagc
    20401 tcctcgctga gtaacgatta cgatcggcgt atcaccttct gctaacacaa gcagcccgtt
    20461 gataatccta tctccatctt tcaaattcat agacttcact ccagctgcct ttgctccaac
    20521 aacgggaaca tctgttaaag gatagcgcaa acccattcct tgatgcgtta ctaagaaaac
    20581 atctaagcct ggctgatctt ctttcagata cacagcaatc agttcatctt cttccgcctt
    20641 tagcttcata cagctgagcg gacggctctt ataggtgcgc catggttcga actctgtcat
    20701 tcgtgtttgc ttgatcaatc cattcttgct gatgaataca aatgttttct ctgcttgaat
    20761 tttttgatat gggaaaacag ctaaaatcga ttcgtctaca gacagattgg agattgtttg
    20821 agaaatgtgc tcaccagcat ctttccattt caaatcgggt agttcatgga cagggcggta
    20881 gatcacattt gctttatttg tgatcagaag aacatggtcc aatgtactca attcacctga
    20941 gtatagcaag tagtcgcctt ctcgcatccc gatttcttct ggtttggaag cactataaga
    21001 acgaaggctt gttcgcttca catagccttc tcgcgtcaca ctgacaatta cgtcttcttg
    21061 tgccactaag acttgcgtat caattttgat ttcttcgatt tcgtcttcga ttgtcgttag
    21121 acgagcggaa gaataatttt ttttcacttc tcgcaattct tttttcataa cagagaaaag
    21181 ctctttgtca tttgaaagga ttttattcaa ctctgtgatt tgcgcaatca agctttcgga
    21241 ttccttttga agctctgtga tatccgtgtt cgtcaaacgg tataattgca atgtgacaat
    21301 tgcttctgct tgttcttctg taaattgaaa gacgtcgact aaattctttt tggcgtcttt
    21361 tttgtcttta ctttcacgaa tcgttgcgat tacttcatcc agaatcgata aagctttcat
    21421 caatccttct acgatatgct gtcttttttg agctttggct aaatcgaatt gactacgttt
    21481 tgttatgact tgctttcgat gctcgatata acttcttaaa atgtctttca gtccaacttg
    21541 atgcggagtc atatggtcga tagcgaccat attgaaattg taattgatct gtagctcagt
    21601 gtttttgaac aaataattca aaattccttg agcattggcg tcttttttca attcgacgac
    21661 aatttgcaga cctgttctat cactttcatc tcgtacttca gctattccat cgattttctt
    21721 attcaaacgg atctcatcca tttttttcac caaggtcgct ttatttactt catatgggat
    21781 ttctgtgatc acgatttgct gtttattacc cttcattgcc tctatctttg ttttcgaacg
    21841 aagaataact tttcctcgtc ctgtttcata agcttttttg atttcatctt ttccttgcaa
    21901 gatccctcct gttggaaagt caggtccagg aatatattcc atcaattttt caaggctcgc
    21961 ttgtggatga tcgatcatat agattgtgcc atcgatcact tcagctaagt tatgagtcgg
    22021 gatttctgtt gcatatccag cagatatacc tgtcgatcca ttgacgagaa ggtttgggta
    22081 tcgagctgga aggactgttg gttctttttc tgtatcatca aaattccaca cgagatcgac
    22141 tgtttctttc tcgatatctg ctagcatctc tccacttaac ttggataatc tggcttcagt
    22201 ataacgcatc gcagcaggtg gatcaccgtc catactcccg ttattcccgt gcatttcgat
    22261 cagtacttcc cgcaacttcc agtcttggct catgcggacc atcgcttcat aaatactgcg
    22321 gtcaccatga gggtgataat tccccatgat atttccgaca gattttgctg atttacggaa
    22381 tcctttgtca tacgtattgc catctttgtt catggcaaat aatatccggc gttgcactgg
    22441 cttcaatcca tcacggatat caggcagtgc acgttcttgg ataatatatt tggaataacg
    22501 tccgaagcgg tcacccatca cttcttctaa agttaattct tgtacttctt gtcgattttc
    22561 catcaggtga cctcctattc tacttcaaat aactgattgt tttcattttt atctgctcgt
    22621 tcttcatcta ataaatcatt agaaactgaa ggagagattt cagtatcctc ttttttatct
    22681 aagatactgc catcttcttc gagcgtaaat tgcacgtgat tttcgatcca ttttcttcga
    22741 ggttctactt tgtcacccat cagtgtggtt actcgacgtt cagcttgcgc agcatcatcg
    22801 atacggacgc ggatcaacgt acgggaagtc ggatccatcg ttgtttccca caattgttcc
    22861 gcattcatct ctccaagtcc tttatatcgc tgcagcatat atcctttgcc gactttttta
    22921 atcattgctg ccaattcgtc atctgtccac gcatattcaa tgacttgctt ctttccttgt
    22981 cctttggata ctttatataa aggcggtaac gcgatatata ctttcccagc ttcaatcagc
    23041 ggtttcatat aacgataaaa gaaagtaagc agtaatactt gaatatgggc gccgtctgta
    23101 tccgcatcgg tcatgatgat tactttgtcg taattacagt cttcgataga aaactcagga
    23161 ccgactcctg caccaatcgt atagatcatc gtattgattt cttcattttt caagatatct
    23221 tgcattttag ctttttcggt attgatgact tttcctcgta aaggtagaat tgcttggaac
    23281 tttctgtctc ttccttgttt agcagatccg ccagcagaat ccccttcgac taaatacaat
    23341 tcatttttct tagggtttcg ggattgtgcg ggtgtcaatt ttcctgaaag aagagattcg
    23401 ccttttttac gcttcttccc atttcggctt tcttctctgg ctttacgtgc tgcttctctc
    23461 gcttctctgg ctttgattgc tttacggacc aacatctggc tcatttcgct attttcctgc
    23521 aaataaaagc ccatttgttc actgatgacg ttatctacta cggtacgagc aactggcgta
    23581 ccaagttttt cttttgtttg cccttcaaat tgcagaagat tttcaggtac acggatagaa
    23641 agtacagcag ctaacccttc tctgaaatca ctaccttcca gattcttgtc ccgttctttc
    23701 agcaaaccaa cttttctcgc atattcatta taagatttcg tcatcgccgc tttcatacca
    23761 gcttcatggg ttcccccatc ttttgtccga acgttattga caaaagataa aacattttct
    23821 gaataaccgt cattgtattg ataagcgacc tctacttcga taccttcttt ttctcctgaa
    23881 aaataaacaa ctggtgtcaa tgtatctttt tcttcattca gataatcaac aaattctttg
    23941 attccttctt cgtaatgaaa aatttccttg actggttctt ctccacgaag atcagaaaga
    24001 gagattttaa cgccttttag aagaaaggca gattctctta accgttcggc taaaatttca
    24061 tatgaaaatt tcgttgtact gaatattgta tcatctggta aaaaatggac agtcgtcccg
    24121 tttgcttttt ttgtttttcc tatctttttc aaagtaccgt caggtttacc gccgttttta
    24181 aatttctgct gatactctac gccatctcta acgatcgtta caattagcca tttcgataag
    24241 gcatttacca cactggctcc tacaccgtgc agtccaccag aagttttgta ccctccttga
    24301 ccaaactttc cgccagcatg cagcacagta aaaatcactt caactgttgg tattccggaa
    24361 gcatgcattc ctacaggcat cccacgtccg ctatctgcaa cagttatact attgtcttca
    24421 tggatggtca catcaatctc actgccataa cctgataatg cttcgtcaac tgcattatcg
    24481 acgatttcat agactaaatg atgcaagcct cggctgtcag tggatccaat gtacatccct
    24541 ggtctttttc taactgcctc taatccttcc aatacttgga tggacgagtc attgtattcg
    24601 ttgtttactt tttttgccaa ggcaaacgct ccttcaataa tactttgcgg caaaaccgca
    24661 ccacgattat catactagaa aaggtcgtga aaaaaaagag ggtagacgag aaatcaattt
    24721 taattatctc ctaaccgctt tttcgacatt cccctctatc gatcatctct tctttgtggt
    24781 catttttatt tttggttttc tttcaactta ccaagttcaa tcttaataca acgatccatt
    24841 acgatatctg tacgtcctgc ttttcttagc atctcagctg cttctttgtt ttcaatccct
    24901 aattgtgccc agaacacttt agcatcagtt ttcaaaaaat cacctgctat ttcaggtaaa
    24961 aattcactcc gtctaaatac atctacgata tcgattggat catcgatttc tgtcaaattc
    25021 gcatagactt tctcgcctag aatttcttca ccagccaata atggatttac tggtatgatt
    25081 ttatatccat tgtcttgcaa tacctgtgca accatgtaac ttgttcgttc tggtttattg
    25141 ctcaagccta cgacggcaat attttttgct tcttccaaat agttgaaaat cgtttcttgt
    25201 gttggatttt ttacagacat ttctgccacc ccttgctttt ctttttattc catctattat
    25261 acttgtatct ttcatttttt caattgttcc tgttacctat tcatgctaaa ataaaggata
    25321 gttttcaact tgaaagaggg taaaaaatga aattgatctt attacttatt ttagcatatc
    25381 ttttaggttc tattccttcc ggtgtctgga ttggcaaatt gtttttcaaa aaagatatcc
    25441 gacaacacgg gagcggtaat actggaacga ccaatacatt tcgtgtatta ggcaaaacag
    25501 ccgggattgt tgtcctgctt atggatattt taaaaggtac attggctact tgtttgccga
    25561 tgatcttcca cctttctggg attaaccctc tttggtttgg ggtatgcgcc attcttggcc
    25621 atacatttcc gatttttgct gggttcaaag gaggaaaagc tgttgcgaca agtgcaggaa
    25681 tgcttttagg atttaatcct gtattttttg tttattcttg tatcatcttt atcgttagtt
    25741 tattttgtac aagtatggtg agtttgagca gtatgatcag tgcagtattg atcactttat
    25801 ctacgatcgt ccttccttat gtagctcctg tgattttggc taagcccaat tggttactga
    25861 cgatcattgc ctttttagtt tcttccttta tcttttatcg gcataaagac aatatcaagc
    25921 gtatcagaaa ccatactgaa agtcgaatcc cgtttggttt gaatagcagc aagaaaaaat
    25981 cctgatactt tttcattcca aagagataaa acgggagtgt gacaaatcga tgtcacactc
    26041 ccttattact tacaggatag cttttttaca caccttggta aacggtgttc cctgctaact
    26101 tctcggtcac aacctcttac ttcactgtga tggaatattt tgtcttgaag atttcatggg
    26161 cacctagttg attgatccct actttttcca tcaagtttcc actactgttc accgtgtcag
    26221 cgattccgca ccacggttcg atacaaacga aaggcgcttc ttttggataa ggagaccaga
    26281 tccctacata aggcatatct ttatagctta aagttacgct atgggaagat ttttcacttc
    26341 gaatggcgta cgcatttaga ccccttgttt caaaaaccaa ggcatcttgc tcaaaaagtt
    26401 cacgattcaa agccagattc gtatttgtct gacctaacgt cttttgatcc atatcgatga
    26461 atgggccttt caatggtagg cgtaatcttg atttcaatgg agaaaatgcc agatagaaat
    26521 cttcaaatga caatccttct tcaagaggta cattgaaagc aggatgtcca ccgatagaaa
    26581 agaacatttc tttttcaccg gtatttgcca cttgataatg aaccgtgatg ccgtctcctc
    26641 ctaattcata cctgatcact aattcaaaat caaatggata cttttctaat gtctccggcg
    26701 tactccttaa gatgaaacta gccatttctt gtccatgttc aatgacttca aaatccatat
    26761 cccgagcaaa tccgtgttgc cccatttgat atgttttccc ttgataaaca tactgatcat
    26821 cttttaaacg tccaacaaaa gggaataaaa ccggtgcatg tctgccccaa tacgctggat
    26881 caccttgcca gatatactct aaaccatgtt cttttgattt cagactgatc aattccgcac
    26941 cgtgttcagc gatttcggcg atcagctggt cattttgaat aataacagtc attgccattc
    27001 ctccaacttc gacttacttt ttaaaatggg aaggctggaa caaacatttc tatctgcccc
    27061 agtcccctta cttctaataa ctgtgttttg ctaactcgtc tttaaaacgg gaattcaata
    27121 cttttaaata agttcccttc atgcctaacg aacgagattc aatgattcca gctgattcca
    27181 attttcgtaa agcattgacg atcacagaac gagtgatgcc gatactatcg gcaatattag
    27241 aagcagtcag acgaccttca tctccatcca atgcttcaaa gatagcttgt acagccttca
    27301 attcgctata agataatgtg ttgattgcca tttggacagc cgttgcactc cgtacatctg
    27361 cttcgatgct tcgagacttc tgataaagga tctgcatacc gacaaccgta gctccatatt
    27421 ctgctaagac tagatcgtca tctgcaaacg attgttccat acgagataag ataatcgtac
    27481 ccaaacgttc gccagctcca aagatcggaa cgacagtcgt cagaccaaat ggatatttct
    27541 ctcttgactc taccggaaat gcagtaatat cactagtaat tgggatatta gcttcagttt
    27601 tcatcaactg tgccactgta tcagtataag attcaggaaa ttgtttctca acaaacattg
    27661 ttttgattcg ttcattgttc acatcgtgac gttcattata acctagtaat actccgtcac
    27721 ttcctatgat ataggtgttg ctgtcaagaa taccacctag tgtgacagcc attttatcgt
    27781 aaggcaaatc tgcttgcatg tcaaaagtat tcttttgctg caaaagtgtg tttatcttgc
    27841 gggttttcgt tagcaagtct gtcattgtcg tcctccgatt ttttataaaa tatagcgact
    27901 taaatcttca tttgccgcaa ttgagccaag tttgtcatcc acatatgctt ctgtgatcgt
    27961 tatttctccc atttgcatat ctggtgcttc aaataataga tcttctagta gtttttccaa
    28021 aatggtatgc agtctgcgtg cgccgatatt atctgtgtca cggttgacat cgaaagcgat
    28081 ttgagcgatc cgttcaattg cttcttttgt aaacgtaact gacacatttt ctgtaccgat
    28141 caacgcgacg tattgtttta ctaaagcatt atttggttcg gtcagaatct tcacaaaatc
    28201 ttcagctgtc aaatcatcta attctacccg tatcgggaag cgtccttgaa gttctggaat
    28261 tagatcactt ggtttagaca aatggaaagc tcctgaagca atgaatagga cgtgatctgt
    28321 ttggatgatc ccgtattttg tgtttacttg tgaaccttcg acaattggca atatgtctct
    28381 ttggacgcct tctctggata cttctccaga ttgctgtgat ttagaagtga tcttatcgat
    28441 ttcatcgata aaaataattc cactttcttc cgctaatttg attgcctcac tatggatatc
    28501 agcatccttg actaattttg ctgattcttc cttgatcaat aactctaaag cttctttgac
    28561 tgttaccgtc cgttccactt ttttcttcgg tgtcaaagca cctagagttt catttaggtc
    28621 gatccccatt tgctccaatc cattgttcat cattggtgca ggtttctttt ggtcttcaac
    28681 ttcaatggtt acttcacgac tatccagtaa acccttttgt agttgttcca agactgtacg
    28741 gcgattgatt cgaatatctt ctgtcacttc ttcttgcggt tcagcggtat tttgctgact
    28801 agcattaaaa agattcatca tttgttcaaa ttgattagtg gattgtttct gttctttttt
    28861 gataccaggt accaagattt ttaccaaacg tttttctgca ttttttttag cacttgtgta
    28921 tacacggctg tactgctctt tttctacgat ttggatcgca ttttctacta aatcccgaac
    28981 cattgattct acatctcttc cgacataacc gacttcagta aattttgttg cttcgacttt
    29041 gacaaatggt gcattgacga tttttgccaa acgtcgtgcg atttctgttt taccgacccc
    29101 tgtaggtccg atcatcagca tattttttgg tgtaatatct tgttgcattt tttcatctaa
    29161 ctgcaaacgg cgatagcggt tacgcaaagc aacggcaaca gatttttttg ctgcgtgctg
    29221 tcctacgata tattcatcta attcacttac gatttgtctt ggtgtttgtt ttaattcata
    29281 catatctact cacctcttac agttcttcta cgataatatt atgattagta aagatacaga
    29341 tatctgcggc aatatttaac gcattttctg caatttcttt cgcactcatg tcgttttgag
    29401 catagttttt cattgctcgg gctgcagcca acgcaaaatt ccctcctgat ccgatagcta
    29461 agataccatc atctggtgtg ataacttctc ctgtacctga aacaagaagc atttctttat
    29521 cattcattac gatcagcatc gcttcaagtt tttgcataga ttgctgtgtc cgccattctt
    29581 gagctaattc gacagctgcg cgttgtagat tcccgttata ttcgtttagt ttgccttcaa
    29641 atttttcttc taaagtaaac gcatctgcaa cacttccggc gaaacctaca acaacttctc
    29701 cattgtagat acgtcgtact ttctttgcag tgcctttcat gacaacagat tcacccattg
    29761 ttacttggcc atctccagcc atagcaaatt tcccgtcttt ttcgacggca cagatagtgg
    29821 ttgaatgaaa tggtgattcg accatgtttg tttcctccta aatttgttct tttccttatt
    29881 tttacgctct tggatggaaa gaacgatagt ttttttgtaa actttctttt gttacatggg
    29941 catagatctg ggtagtcgat aaattggcat gtcctagcaa ttcttggact gtccgcaaat
    30001 ctgccccatt atttaataaa tgagtagcaa atgtatggcg cagcatgtga ggatggatat
    30061 cactatccaa actgcttttt ttgatcattt gattcaagac atattcaatt ccagtaggcg
    30121 tgattggttt tccatgatga ttcacaaaaa cgatatcatg ctcttcgtga tattttgtca
    30181 tcaatacctg gcggccttcc ctgaaatatt cttgcagagc atcttgagca aaagaaccta
    30241 gaggagcata tctctctttg ttccctttac catggatcaa catgacctct gaatcaaaat
    30301 caatgtctga taccgacaaa ctgctacact cacttaaacg gatgcctgac ccgtaaagga
    30361 cttccagtaa agcgcggttc ctcaaatcca aaggactatc tcctgctacg ctgtcaaaaa
    30421 gtgcttgcat ttcattttca taaaaaaaac gtggcagtct caaatttttc tttttgagat
    30481 gaacgtaaga aaatggattt tcttttatca cttcttcttt gagaagatac tgataaaacg
    30541 aacgtaggct ggctatcttg cgactgatag aatttctgct atagctagga cgatgatcag
    30601 ctaatgcact caaatagaca cgaaccgtaa aatattccgc cttcagcaga tcagcgtctc
    30661 cagattcctc caaaaaatgt ttgaaatgga gaatgtcttc ttcatatgct tctttcgttt
    30721 tttctgaata tcctctttcg acaatcaaat aacgcaaaaa tttttgttcc cacgtggctt
    30781 ctgtcatttt ctcaccttcc ttttgtccca gagaataatt tagcacagcg tttataaaaa
    30841 gacaaataaa ttgtcagaat ttaaccaata tttctaagtt tgttcacaat tggcgcaatt
    30901 cctcacattt tgtttgagaa attcttaaga aaaacgcaag aaaaagagcg agactatccg
    30961 tctcaccctc ttcatggagt ttccttactc atttgggatt gcagtttttt ctaattctgt
    31021 ttttattgcc tcattagctt ttaaagctcg ttgtgcaatt gcttcatatc ttgctttttt
    31081 gtctcgaatg cgctctggca gttcaggaaa cagaccatag ttggcattca tcggttggaa
    31141 atgttttcct tccgcatgag taatgtagta agccatactt ccaatagctg tttcttgtgg
    31201 aaactcgact ggttccattt cttttgctaa gcgtgcggcg ttgattccag caagtagtcc
    31261 gctcgctgca ctttcaacat agccttctac accagtcatc tgacctgcaa agaacagatc
    31321 ttcgcgtttc gttgattggt aagtcggttt cagcaattct ggcgaattca taaagctgtt
    31381 tcggtgcatc acaccatagc gaacgaattc agcattttct aacccaggaa tcatccggaa
    31441 aacacgtttt tgctcgcccc atttcaagtg agtctgaaag cccacgatgt tgtataaaga
    31501 agctgcagca ttgtcttgac gcagctggat aacagcataa ggacgtttgc cagttttagg
    31561 atcttccaat ccaactggtt tcataggtcc gaaaagcatc gttttgaatc ctcgctgtgc
    31621 cattacttca attggcatac atccttcaaa atatttttct ttctcaaaag atttaagttc
    31681 tgccacttcg gcattgatta gtgcctcatg gaaagctttg aattcctctt ctgtcatcgg
    31741 acagttcaag taagctgctt ctcctttgtc atagcgggac ttcaaatata ctttatccat
    31801 atcgatcgta gctttgtcta cgattggtgc agctgcatca tagaaataaa aaccatctga
    31861 accattgaat gctttgatgc tttctgctaa tggatgagaa gtcaaaggtc ctgttgctac
    31921 tacggtgatt ccttccggta tttcagtaat ttcttcgttt atcactgtta ctaatggatg
    31981 ctctttgact tttcgggtaa ttgtttcaga aaaagaatcg cggtcaacag ctaaggcacc
    32041 accagctggc actgctgttt catctgccga agtgattact accgaattca atcggcgcat
    32101 ttcttctttc aataacccta ctgcattagc caaactgttt cctcttaatg aattggaaca
    32161 tactagttca gcaaaattac ttgtgtgatg cgcctcagtc gatttgactg gacgcatttc
    32221 atataaacgg acagggactc ctgcttgtgc gatctgccaa gcagcttcac ttccggcaag
    32281 ccctgctcca acaacattta ccattttcaa tgaaagaacc tcttttctat tatttttgga
    32341 tgtcttcttc gtaatctcca tttacacaga ctacttgttt accgcctttg actttcttct
    32401 cgaccagata tccgtcacat tttggacaat ttctgcccac tggtttatcc caagaagtga
    32461 attcacattc tgggaaacgg gaacaaccat agaatagtct gtttttctta gattttcgtt
    32521 caatgacttg tccttcatga catactgggc aagtcacacc gatttctttt acgattggtt
    32581 tcgtattccg acaatcagga aaattactgc aagcataaaa cttcccatat ttccctaatt
    32641 ttatcaccat cggatgtcca cagacatcac aatcgaatcc tgctggttca tctttgattt
    32701 gaattttttc gattttttct tccgcattcg tcagttcttt ttcaaacggt ttatagaaac
    32761 gatcaaccac tttgacccac gcttccgtgc cttcttcaat tttatccaaa tctccttcca
    32821 tttctgctgt gaaatggata tcgacgattt gagggaagaa ttcacagatt aatgaattga
    32881 caatttctcc tagttcagtc ggctcaaatc ttttttgtgt caattttaca tagtagcgtc
    32941 tttggatcgt atcaagtgtt ggtgcataag tggaaggtcg ccctaccccg ttttcttcta
    33001 gtgcacgaat caacgtagcc tcactaaaac gtgctggtgg ttgagtaaaa tgttgttttg
    33061 gttcaatatc aacagattgg actacatcgc cttcttcaag ttctggaaga atattttctt
    33121 tctcttcttt gccgtcatct ctaccttcca catacacttg catgaatcct ttgaatttga
    33181 tttttgaacc gttcgcaatg aagatgacac cattttgttc taatgtcact ttcattgtat
    33241 ctaaaatagc tggtgtcatc tgactagcca caaaacgtga ccagatcaat gtataaagtt
    33301 tcaattgatc tttatctaaa tattgtttga gttcatctgg tgtacgaagc acactcgttg
    33361 gacgcacagc ttcatgggca tcttgtgcgc cttgagcatt tttgatcttt cggtgatcat
    33421 gtctagaaaa ttcttttcca tacgtatctt caataaatgc tgcggcctct gcttttgctg
    33481 tatcggcaat ccgggtagaa tcagtacgca tgtatgtaat caagccgact gttccttgtt
    33541 tacccaaagt gattccttca tataattgct gtgctaccat catggttttt cttgttcgaa
    33601 agtttaactt acgggcagct tcttgttgca ggctacttgt agtgaaagga ttcgcagggt
    33661 ttcttttgcg ctctttcttt tctacttttt taacttcgaa gtctgttcca tcaattctag
    33721 cagtgacttc ttttacactt tgtgcatctg gtaatttctt tttcttaccg tcgattcccc
    33781 agaaatttgc tttgaatttc ttgcgagctt ttttaaaatt accgtcgatg ctccaatatt
    33841 cttctggaac aaattctcgg atctcttttt cacggtcaat aataattttt aaagcgacag
    33901 attggacacg tcctgcactt aaccctttct tgaccttacg ccagagaata ggactgatcg
    33961 aataaccaac gattctgtct aaggtacgac gagcttgctg tgcatctact aaatctacat
    34021 cgatcgttct tggttcctta aaagctgctt tgactgcgtc ttttgtgatt tcattgaaga
    34081 ccacccgatt tttttctttc agatccaacc caagtaggta cgctaaatgc caagcaatcg
    34141 cttctccttc tctatccggg tcacttgcca agtaaacttt ttgagctttt tttgctgcgg
    34201 ctttcaggct tttgatgaca tcgccttttc cgcgtataga aatataatgg ggttcataat
    34261 tgttttcaaa atcgataccc attttacttt tcggtaaatc tcttatatgg ccaacactgg
    34321 ccattacttt atagtttcgt ccaagatatt tctcaattgt cttggctttt gcaggtgatt
    34381 ctacaatcac taaatattta tacgccatta ctgaatggac tcctttccat tgtcgtgttc
    34441 ctactattaa aagaggattc atacaaatcg tagctaatca tatccattca gaaaacgttt
    34501 gtcaagaaaa gcaaatgaaa agtatggtgt tattctttaa attctgccaa gatatcttca
    34561 cccgaaaccg tgcaaactgc accgtcttgg atcaaatgat gacatccggt agaatgttct
    34621 gttaaaatat ttcctggaac agaaaaaact gttcttccaa gatccaatgc ttgctgtgct
    34681 gttatcagag agccgctttt tttcttggct tcaaagacac atgttccttt agacaaagct
    34741 gcgataatcc gatttctcat tggaaaacaa tgctttttcg ggccggattg aggaggatat
    34801 tcactcaaaa gaagatgatc atttgcaatc acctcttgca agtcacgatt ttcagcagga
    34861 tagacttgct ctatccctgt tccaatgact gcgattgtcc gtccaccatg agagattgct
    34921 gcttggtgac cgtatgtatc tatccctttc gcgcagccac tgacgatcac atatccagct
    34981 tctatcactg gttttagtaa ataataaact gtttttttgg ctaaagcaga agtgtgtctt
    35041 ccaccaacaa cgcttaacat gttacaagaa agtaaagata gatcaccatt ataaaacagt
    35101 acaattggcg gataggtcaa gtgaagcagc tgagggggat atttgggatc aaagcaagtg
    35161 ataaacgact gtttcgattg gattttgccc cagttagcgt gaatttcctg ccaacttttg
    35221 cgaaacagct cccggtgttt ggttatttta cctatcttaa tgatgtcttc aaatccgatt
    35281 tctttataag gatactgcat catccagtta gcaagtgccc attttccttg actactgatt
    35341 ccttttgtat aagcaagttt caataaattt tcttctattt gatacatcat aaaaccgcct
    35401 ttcgtctctc tttctaataa gatacgtaaa agcggctgtt ttttattgta aatatttttt
    35461 tacaggtgca aaacttttac gatggattgg cgtaattcct tgtcgttcaa gtccttctaa
    35521 gtgttccttt gttccataac ctgcattatg cgagaaacca tatccaggat aaagcacgtc
    35581 atattctttc atcaattggt ctcgatatac tttagcaatg atacttgcag cagcaatcga
    35641 cacagatcgt gcatcccctt tgattatttt ttcctgctga ataggcaaat ccaaggtcat
    35701 cgcatcgatc aacaaatagc ttggtacaat ggacaatttt tctactgctt ggatcatcgc
    35761 taatttactt gcttcataaa tattcacatc atcgattgtc tgctgatcaa cgatcccgat
    35821 cccgattgcc agggcttttt cttgaacttg ttgataaaga agttctcgct ttttctctga
    35881 taattgtttg gagtcattca gccctaatat gatctcattt ttcggcaaga taactgcagc
    35941 tgccacgaca ggtccagcta atggtcctct tcctacttca tcaatgccgg caatccattg
    36001 gtgaccttgt gcgtaccctt gcttttcaaa gcaaaacatt tcttcatatt tttccttaat
    36061 taatgcttcc tttttcaatc gtcgttcaaa agaagccagt gcttgctgca cgcctttgcg
    36121 tggatcattt cttaatgatt ctagtgtagg atcatctatt ctctgtatgc ttttcaatcg
    36181 ttctttgata ctttggatgg attcagtcat cacttttttg ctccatttct tcgcaacgat
    36241 ccagtgtgta tcgtccaagt tttccttgac gaatatcatg cacgactaat tcgctgccac
    36301 gatcatagtc ttctttatac ccacgttttg ctgtaatatc catcagtaat tccggtgcca
    36361 atttttcttc ttgctcgatt gataaaccat aacgatcgct gattcttcct gaataatatc
    36421 gggcaaaaaa gtctaatccg taaatcgcta aatcatctaa atggacaagc tggtctttga
    36481 tcgcgcctgt cagcgctagt tttttgccga tttcttcatc ttcaaatttg ggccataaaa
    36541 tccctggtgt gtctaacaat tccaactcat tacctaaacg aagccactgc tgaccttttg
    36601 ttacgccagg cttatttcct gtctgtgcaa ttttcttgcc tactagtcga ttcatcaacg
    36661 tagatttccc aacatttgga atacctaaga ccattgcccg aatagcgcga ggcttgatcc
    36721 cccgagcagc atctcttgcc cgtttttctt tcaatgcttc tttagccgca ggtactagac
    36781 ctttaattcc tttactttct tgtgccgtga tagctaatgc aagatggcct tgtttacgaa
    36841 aatattcttg ccacagcttt gtctgttgtg gatctgccag atccgcttta ttcaataaaa
    36901 caaggcgagg tttttgctga atgatctgat ctagcattgg attacgagaa gataaaggta
    36961 atcgtgcgtc taccaattca aatacgatgt ccacatattt cattttttca gaaacttctc
    37021 gtcgggcttt tgccatatgt cccggaaacc attggatggt cattgttctc tccattcctt
    37081 tcataccgga atattccccg gctttacttg ttttttttct ttttcaatat aaattcactt
    37141 ttttattata aagaaattcc atctattagt taagtgaaag tgaaccagtc ctcattctat
    37201 cacatcttca cttttttgat gatgtaaatt tttattttgg tttctatata tgaatctaaa
    37261 tttttgacaa cgtttcctaa ataagagatg atagacgaac aaaacagaaa ggagacgaaa
    37321 caaatgaata gtatggtttt tgttaatttt cctgttgctg atgtcaatcg ttccgttgct
    37381 ttttatgaaa agctgggttt taaaaaaaat gaagaatttt ctactgaaga agctggttca
    37441 atggtatggg atgacacttt ttgggtaatg cttctctctc atgattttta taaaaaattt
    37501 ataaaagata aagaaatagc tgataataaa aaaacaagtg gtgctttgat ttcgtttagt
    37561 gttgaaaacg cggatgaagt aaaaaaattt gctcaaattg caaaagaaaa tggtggtact
    37621 ttccacgaag tagatatggg aatgcctgaa gaagaaatgt tttcattaga agtccaagat
    37681 ctagacggaa atacattaga acccgtatgg atgaagatgt aagcagataa ctaatctgac
    37741 gttttttata acaaaaaaac aaaggcggat gatacgataa gtattctccg cctctatttt
    37801 tttcattcgt acaatctctt ttttacagtt tataaaagca gtcttctcac ccttttttta
    37861 attgttagat gccagtgtat gtaaatcacc aatgtgtcat gacacattgg tgattttatg
    37921 gtatcctttt attattaact gacaaaggag agaatatttt gaacactttg attgcaaaag
    37981 acgtttttag attgcccgaa atgaatgcaa gattgttgtc gacctctgct cttttattag
    38041 cccttgatgt catcttatac aagatagcga ttggtcctgc ttattttcaa atcacattcg
    38101 gatttttaag tatggcgctt atgggttatc tatatggacc gatctgggct ggtttgcttg
    38161 aaatgttgtc gaacacttta aatttcacga tatttggttc aggaacgttt caagctgctt
    38221 ttcttatcac ggctttcctt ggtggattta ttaatggctt atttttatat cataaaaaga
    38281 taaacctgcc ttccgtcatc gctgcccaat tattgattat gatcccagtc agccttttta
    38341 tcaattcttg gctgatgtct gttttgtttg gtgcagattt caaagtgatt tttctaggta
    38401 gagtcatcag gaatatgatc caaatcccta ttcaaataat ggttgtttat tcatttatca
    38461 aaactttaga ggctagaaaa gtgtttaaac gagctgtttg attttacgta ctaaggtagt
    38521 aaactgattt tggattaaac aataaagcgt gcagtctcaa cagattgcac gctttattgt
    38581 ttctcactgg atccatttta tttcatcaag tggataatag acaaatcttg ctttacctag
    38641 aatatcagtt ttattgatcg tgccaaacga acgactatct tttgaaactc ttcgattatc
    38701 tccaagtaca aaataattat cttccggaag cttggattgt cccgttagct cttgtagtgt
    38761 aaagtccgtc gtataggatg catttgcatg atctgatttc aggtttttag ttagataagg
    38821 ttccttaact actttcccat tgatcttcaa ttgatcattt tggtagctga cattttctcc
    38881 tggaagaccg attactcgtt tgatataaat cgtgccatct gctaattgaa aaacaacaat
    38941 atcaaatcgt ctgatctccg aaaatttttc catcacaacc atatcacctt gcttcaacac
    39001 gttttccatt gaattgcctt ccactggtac cgggatgaat aaaaatcccc gcaacatgaa
    39061 cgcaaggaat acagaaacaa gtaagtattt gaatacaagc cagaagcgat taatatagcg
    39121 ttgttttttt gtcaatctct atcaatccat ccctcgtttt ttcattcttt ctttattata
    39181 agaaaaatat caaaaaaatg cgactagata gctgaaataa aaataattct ttcaattagg
    39241 ctattggaca aaaaactttt ttgatcataa agtacaaaac tatctcttat atgtcatgat
    39301 tattttctaa atatttttcc catgcttcgt caaagatatc catggacggc aaatagtcga
    39361 cattcagttc cagataatta gaaatttctt cgtagctaga gctgtgctta ggaaattgga
    39421 tgtctttacc ggcttctgta gcgaaaacag ataaagcatc atgtgtgcca ccgcctcgca
    39481 gtgtcattaa ataatgaaaa aagcttcgat tcacccttcg ttcctccaat tttcttcgat
    39541 aaaggcagcc cggcgtgcat ggtttctttt aaaattttca ggatgctttt tataatactc
    39601 ttgatggtct tcttctgcta gataaaatgg ttgggctggt tcgatttttg taacgattgg
    39661 ctgttcgaaa cgtccacttt cagctaactg tttcttactt ttttcagcaa tgatccgctg
    39721 ttcttctgtc aaataaaaga tcaccgggcg ataattgtca ccgcgatcct cgaattgtcc
    39781 gaaagcgtca gttggatcag tttgctgcca gtaaagttct actaattgtt catatgaaat
    39841 caatgttgga tcatagataa tctccactgc ttctgtatgt ccagtcgttt ggctttttac
    39901 ttgttcataa gtcggatctg gtacatgccc tccagtatat cctgacatca ctgtatggat
    39961 accaggaagc gtgtcgaaag gctggatcat acaccagaaa cagcctcctg caaatattgc
    40021 tcgttccatt atgattcccc ctctaatact actcatccga tgatttttta ttttcttttg
    40081 tcttgctttc agtactgctg gtgctagttt tgttggtatt tgagttgttt gaagcatcag
    40141 aggtatcttt tggcaaatat agactaaacc gaatgtcatc atttactaag tcgattttat
    40201 ctgctttaat atacatcccg ttttgcattc ggaattgatc caacctaagc atgatagtca
    40261 gatcatctgt attgacttcg atccaatctg gtaatttata gtttctcttg atcatattca
    40321 tcacatcttt gattggcaaa ctcaatgttc cgatagaaag acttctagct tttagttgga
    40381 catttccgtt ttccatcaca tacggatcaa aatataaata gaaatcgata ggaaaaccta
    40441 gtacttcgaa agtaccattg agtagtgctt cattttccaa ataaaattta tattcaatat
    40501 cactatcttt ttgatactca ctaaggaaaa agctgatgat ttgattgact ttttctttat
    40561 ttgaattgat cgttaagaca ggatctcctt gtctttccgt aagagctggt atttctggga
    40621 gttctggttc ccgattagca aaaatacggc tgcctaaaaa tacggtcgaa ccaagtacta
    40681 ccccaagaag gataaggaag gcgaatttcc agtaatttgt tttctttgtt ttgtttgaag
    40741 atacgtcaga tcttgttttc atgtttattg atcatcctcc ttctctagcc attggttttg
    40801 tgtctcgatc agctgatctc tcactgcatt tgccatcaat tgataaccta aattatttgg
    40861 atggaaatga tcttcttcat agagcacatt atttttgatg ccatttagat tttctgtcgt
    40921 gctagttgtt tgatctgttc cttcttctgt gacacctact tcctgattca atccttgata
    40981 aagcaaatca ttgattggga taaagtagca gttcggcatt ttttttgtca ccgtttctgt
    41041 tcctttgttc caattgttaa caattgtctg catgtcagtg atctcaggaa agttcaaata
    41101 aaatggattg tagatgccta aaatgtaaat aggtgcggtt ttattgaggg atcgaaattc
    41161 ttccaatagt tctgtgacat tttcctgata gtttttcagt ggctttttaa aggaattgac
    41221 cgttaacccg aataaattat tttggataac tttcatcaaa tcattaccgc caacagtcag
    41281 tgtgataaaa tcagctgttt tgatattgtt tcgaatttct tcttttgttt ttactcgctt
    41341 taagatctgg tcacttcgtt caccatttac accatagttt tctgtttcaa ccgcggtcaa
    41401 attataacgg tcttttagat catttgcgac aaggggaacg aatcctcctt gcttggtttg
    41461 atctcctact ccttcagtca gcgaatctcc gatagctatg tagtgaagtt tttctttttt
    41521 atttggatct tctctcacag tagttactgg tttcattagt gggccagctt tcggtataga
    41581 agcagctaaa cctgcataga tgatgatcgc aattaataat gggaacaaaa cgaggatcag
    41641 atgtcgtttc gttaatttca ttttttcacc tcttgtattc gattggttct aatgttatca
    41701 aaaattttat caaaaaggta gtctttatga aaaaagaaaa caattatttt cctaatttta
    41761 taaagaaaaa gagaataagc tgcaagcctt tcaggctgag ggcttatcct cttttcgact
    41821 aatctacata atacatgatt gcaaacgcgt ctttaccagt atgcgtagcg ataaccggat
    41881 tcgtatgcag tacagggata tgcatgtccg ggaataattc ttgcaaaccc tctttgaatt
    41941 cttgcgccaa ttcttttgca tccgcatgtg aaataccgat ttgtcgcaca tttggcaatt
    42001 ctttcagttc atttttcaaa ttattaaacc atttatcgaa cgttttcttt cctcgcccct
    42061 tagttacagg gattaattca gaatgggcga aattcatcac cacgcgtatg ttcaaaacac
    42121 tagacagcaa accagttgtt cgactgattc ttccaccttt taccaagtta tctagtgtgg
    42181 atattccgat gtataatttt gtattgtctt gtactttttt gacttcttca aggatttcct
    42241 ctactgtttt accttctttt gctaattcag ctgctcgaat tacctgaaaa gataatcctt
    42301 ggtcagtggt atcactgtcg ataaccgtta ctttcgtttt tgttagatta ctggcttgtc
    42361 ttgctgcttc tactgttcca ctcaagcctt ttgtcatatg gatcgaaatc acttcactgc
    42421 cgtcttctcc tagctcatca tacatcgaga taaattctcc aatcggtggt tggcttgttt
    42481 tgggcagatt ttttgctttt gccatcattt ccataaatct ttcgccgtct agttggtcat
    42541 catcgggata tactgttccg tcgatcatga tggatagcgg catgacacga atgcctaaag
    42601 catcgcgtac atttttttcc atcgtacatg aagaatctgt tacgattttt acatttgtca
    42661 ttattttctc tctctctttc tttaacaaga tgccttattc atgttaaact acatgtaaga
    42721 caatttctat ttttagtata acagacaaat aatgattttt cattaattac acctagtaaa
    42781 ggagccgttt tcatggaaaa aacgaaattt aatcgaacgt atgaaatatt aaatgaagtg
    42841 tttaatgcag tcacccatgg aattgggact ggattgagta tcgcgggact agtggttctt
    42901 ttagtaaaag gtgcccactt gcattcctcc ctccatattg tctcttatgc tatctatgga
    42961 tcgatgatga ttcttttatt tttaacgtct actttgtttc attcattgat ttttacaaaa
    43021 gcaaaaaagg tgttccaagt attcgatcat agttcgattt ttcttttgat tgcaggaagt
    43081 tatactcctt tttgtttgct gagtatccga ggatggctag gttggacttt atttgtatta
    43141 atctggttgt ttgcgattct tggcattata tataaagcgt tgacgttgca taaacgggag
    43201 agagtatcaa aagtatctac gatcatctat atttttatgg ggtggctctg tgtcgtggct
    43261 gctgttcctc tttatcactc tcttggaatg actggtgtta ttttgatggt agcaggcggg
    43321 ctttcttaca cgataggcgc attcttttat agcttgaatt ctatccgcta tatgcatgtt
    43381 gtttggcatt tgtttgttat gttaggtgca ggttttatgt acttctctgt tttattcttt
    43441 acttaataaa aaaaaataaa gctatggatg ggacagctct aaataacagc tagtctatcc
    43501 atagcttttt tatatcgtca attcgcctgt cccgcttttt acaggattct cattacgctg
    43561 atacgtttca aattgatgcg gataacgatt tttttcatct atttctcctt gactagtatt
    43621 gatcagtgac caatcagacc agtcaacagg tggaaaataa gcatctccat cgaatacatg
    43681 atggatgact gtacggtata atatcgtaca aaatggcaga aattcttgat aaacagctga
    43741 ccctccagcg atgaaaaaga taccgtctgt ttctttttcc agagcaagca cgtcttccac
    43801 actatgcatg acatgcacgc cttctgcttt ataggaagaa tctctcgtca aaacaatcgt
    43861 ctgacgattc ggcaacggac ggctacccat cccttcgaaa gtttttcgtc ccatcactag
    43921 tgtatttgct tctgtcattt gtttgaagaa tttcagatca tttggcaaac gccaaggaag
    43981 acggtcttct tttccaatca atccgttttc atcctgtgcc caaattgcag ctaacatttt
    44041 ctcatccttt ctgattctaa acagcgatag gagctttgat cgccggatgt gggtcatatc
    44101 cttctaattg gatatcttcc atctcaaaat caaatactga ctgcttttcc tgattcaaaa
    44161 ctaaagtcgg aaatgcgcgt atttctcggc tcaattgttc tttcatttgt tcgatatgat
    44221 tgatataaag atgagcgtct cctaatgtgt gaacaaattc gccaaccgct aaacctgttt
    44281 catgtgcaat taggtgagtc agcaacgcat aacttgcaat gttgaacgga acgcctagaa
    44341 acacatcccc actgcgttga tacaattggc agcttagccg tccctcttga acataaaatt
    44401 gaaacatcgt atgacaagga ggcaaagcca tgctaggaac atcttccgga ttccatgcag
    44461 aaacgatcaa tcttctagaa tcaggagtct ttttgattgc ttcgatcact tctttgattt
    44521 gatcgataaa gccaccgtct tttgtttccc agtgacgcca ttgatagccg tagatgtgtc
    44581 caagatcgcc gtatgtttca gcaaaggaat catcggttag gatcttctcg caaaaaagct
    44641 tgctctgctt tttatattct tcattgaatt caggatctgt caaacatcta tggccaaaat
    44701 cagtcatatc tggtccatta tagtctggac ttttgatgta gcgttcaaat gcccattcat
    44761 cccaaatatg gtttttattt tgtaatagat agcggatatt cgtatctcct tttaaaaacc
    44821 aaagcaattc gcttttgatc aatccaaaag gtactcgttt cgttgttaac aaaggaaacc
    44881 ctttttgcag atcaaaccgc atttggtatc cgaataagct taatgtgcct gtgcctgttc
    44941 tgtcttcttt acgatgccct tcttccaaca attttcttcc taagtcaaga tacgcttgtt
    45001 ccatcctttt tcctcctaat cttctaccca ttcactcaag tattcccacc gtgccatttt
    45061 ttcttcaagt tgtgcttctg tttcagatac gtcgttttgc aattcttgta atcgagtgaa
    45121 attatctcct tgatgattca tctcttcttg gagcaatgag attttttctt caagttctgc
    45181 aatctcatct tcaatcgttt cccattcttt ttgttccatg taagataatt tcttcttttc
    45241 agcaggttcc ttgctctgta tttttggttt cgctttttca gcaggctgct gggtctcttt
    45301 ttgcttcagt aaataactgg acatgtttcc gtaataaagt tcttgttttc cgtttccttg
    45361 gaatactaat aacttatctg ctaccttgtc caagaaataa cggtcatggg aaacagtgat
    45421 cacagcacct ggaaaacttc taaaataatc ttccaaaatc gtcaatgttg ctatgtccaa
    45481 atcattggtc ggttcatcca ataataaaac attaggttgc tggatcagca gtttcaacaa
    45541 aaataaccgg cgtttttctc cgcctgaaag tttgcctatc agtgtcccat gcataaatct
    45601 tggaaataaa aatcgttcaa gtaattctgc tactccgatt tgactgccat ctgctgtttt
    45661 tgcttcttca gcagcttctt gtaagtaagc aatcatccgt ttatctggtg ccatttcttc
    45721 attttcttgt gtataatacg caagatgaac cgtttcaccg atagacattg taccgctatc
    45781 caggggaatt ctgccagcta gaatattaag caatgtcgat tttcccgcac catttttacc
    45841 agtaatacct agtctttccc gagattgaat cagtaaatct aaatgttcta gcagtgtttg
    45901 gtcatttata cggtaactgc catctttgat ttccagtact tttttcccta aacgttgtgt
    45961 cgctaggttc agctctaaat catcttcttg gtttacttgg tacaagttct cctttagatc
    46021 ttcgaaacgg ttgatccgtg cttgctgctt ggtgccacgt gcctgtacgc cagccctcat
    46081 ccaagctaat tcctgtttat aaagccgctt gcgcttttct tcttgttcta cttctacacg
    46141 atcacgttct gctttttcta agacgtaagc ttcataattt cctttatact cgtataattt
    46201 tccaaatgaa agttcgaaga ttcggtttgc tactctatct agaaaataac gatcgtgggt
    46261 aaccatcaat agtgcgccac ggtattgttt caaatagttt tccaaccact cgatcgcttc
    46321 ataatctaaa tggtttgttg gttcgtctag taatagtaaa tcgggttcat caatcaacac
    46381 ttgcgccaga ctaactcgtt ttttctgacc acctgataaa gtactgattt ttttatctaa
    46441 ttcactgatt cctaattttt gcaaaataat ctttgcgttg gtatctgtag tccaagcatc
    46501 ttctttattc atttgttctt ctgctttagc ataacgtttt tgcacatcag ggtcttctcc
    46561 gttttcccct agttcgatca atgcttcctc atatgctcga acagtttgga tcaaggggct
    46621 gtttccttga aagactgctt ggagaacagt taattcatcg gaaaattcag ctgcttgaga
    46681 aagataacca atacggtaat cagttggata aaacactgtt tgccgatcac cgtcgccgct
    46741 atcgataccg gcaataatat ttaagagact tgttttaccc gtcccgttcg tcccaatcaa
    46801 cccgatacgg tctttttcgt ggaccaaaaa agaaatctgg tcaaacaaat ctttttcacc
    46861 ataagttttt ttgaggtcag ttacttttaa ttctttcaac atgttccctc cttatcttgg
    46921 atattgtatc ataaaaatag tgaacttcag gaaaattttt ttggtatttg ttctttttgt
    46981 gttctcaaaa aaagaacagg accgattcga tcctgttctg atatgttttg ttttttataa
    47041 aacttgaaaa tttttacctt tttattttat tggactcgtt tttttgactt tgctcgaaac
    47101 gcaaatcgga aagaaaagtt atcagatggt ttgttcatcg tataagattc taacaaattg
    47161 atattcctgc gatagctgtt cgcagaaaac gtcgtgattt tctcagcaac ttcgtattca
    47221 tcaatgattt ttctttcaag ataataacct cttagcattt tttttgtaaa cagactatct
    47281 tgctggatca tccacgaaga aatcaaattc cccgagttca tttttattcc ttcaagtttg
    47341 cgttgctgga taaaaaattg aaccatcgtt tcatcataaa tatcagtcaa gttatctaaa
    47401 ctttcaataa tcaactgagt atttttttga tatgtctctc tgatttcttg gatatcttca
    47461 tcactaataa atgtctgacg atcagcatgt cttctttgcc aaaacatttt cggatggaga
    47521 acgattcgca ttaacctacg gacaacaatg aaccaaaagc cgataaaaga caaaatctgg
    47581 cttgtgatag aatgttcgaa tcgagaaaga aaacgggagt aaaaacgata accattagaa
    47641 gaaagcttgc ccgaacgata actttcatcc aatccatccc gttcgatcga caaaatcagt
    47701 gcttgtattt cttgtacttc ttgcttttca tcatcggtta aatcttccaa gtacagatca
    47761 cgtatccgtt cttgataatt ttctatcact gcttcagtgg ctaaaaattc ttttgtatta
    47821 cgatcaattt cctttatttc ttttcgcaaa acttcaacga cctcttctaa aatcatcaaa
    47881 gcattcaaat ccgtgctttt cgcttcctct gtttccgtca gcataggcaa gacaatgatc
    47941 cctatgacca aagtcactaa gatgacaccc gccgtcagaa acaaaagcaa agaccgctgg
    48001 taaaacatca tattattgat tgaaaatggc aaaataaaaa tcgtagccaa actgactgtt
    48061 ccctttacac cgccaaatgt taagatcaag atctcgttca actgtttttt gaacttttta
    48121 ctgccatctt tgaatacata aaaaagactg atagaaatga atctgatcac aaaaagcatg
    48181 acagagatta atacgataat cgctaataaa agaccgtttg gatataaacc gtctccccat
    48241 actggcgaga acacttgcgt caattcgatt cctaaaaata taaatactaa tgcattcagt
    48301 gtaaaagcaa tagttgtcca tgtactgtgg gacaagctcg acaattctgc atcaaacaca
    48361 gagattttcc taaaaccaga agcttggaga atacctgctg ccactgcagc aatgattcca
    48421 gatacatcaa atgcctctgc caatacatag gctagaaaag gaagcaaaag ttcgatcagc
    48481 agataccctg tgacatcttg tgctgatgct ttttcgatca aatgaattat tttttgtttg
    48541 aaccagacaa gcaaaaagcc gatcaaagct cccccaatac tagaaactac tagactcatc
    48601 cccgcgttta ctgctgaaaa agatccggtg atcagcgcac caagagcaaa ctggaaggca
    48661 gtaacaccag atgcatcatt caaaagacct tctccttcca aaatatgcat cgccttttta
    48721 gggatattca ctcgtccaga aagagaactg actgctaccg catctgtcgg acctaacgct
    48781 gctccaaaag caaagcaggc agcaagtggg acacttggca acaggaatga taacgtcgca
    48841 cctaccccga caagggtcaa aatgactcct ccaaatgcca agaacaaaat tgtcccgaaa
    48901 tttttgagaa tcgaagaaat atctgctttt tctccttctc taaataacaa tggtgcaata
    48961 atcataacta agaacaattc aggttcaaaa tcgattgatt gtccccactc tgtcagacct
    49021 aataccactc caaggaaaat ttggatcagt ggagctggaa tcattggcaa aatgcggcta
    49081 gctatgttcg ataaggtgat cgttactgca aatacaataa ctaactcagc aaattccatc
    49141 gtccatcccc tttatttttt attttgtgta agcattttta aaaattgttc ttttttcttt
    49201 aaaattttgg tttcgactac ttcaacctgt gtttggttac cctctaattc acactgtaca
    49261 cgttttgact ccaaacgaag cagatcatgt ttcagacgtt ccaactgacg atacatcaat
    49321 atatttcttt tacgcaactt acaaagtatt tgacggcggt catccatcga aagggcgcca
    49381 tagcgatcat gcaacagttt tttttgttgt tcaaaactct ctctcatttt ccagatctcc
    49441 tatacacaaa cttccatcat tatagcaaag ttctttttgg atgaaaaata tttcattgtt
    49501 tcatcgttta ttgctagtta gaaatttcca taaatttttc aacgcctcat tctattccaa
    49561 atagtatcag atatcaagta aaacttaaga aagataaaag tttctgacaa aagaatgttt
    49621 acttttccat tcctgccttt cttgttcttt tatcacaaca aaaaaatggg gctgcgacaa
    49681 aaatcttgtc acagtccctt attctgaata aacggtgtgg aagagacagc ctatcagtag
    49741 taataagaca catctctttc acagcttcat ttttgcgtaa aaatcgattt attcgaaaaa
    49801 ttccccgtat acatcttcta cacgttgatc tggattgatc acgatataca agttacctgt
    49861 tttttcgctg atttttgttg attgatattg attgtctaat ccttctgttc ctttatctag
    49921 atatgggaag tacacaagat caggtgtgcc atttcggtaa aggatttcca ttcgctcgat
    49981 atcttcgtat ttcaatgcgc gattaaacat gcccatttcc agtccgccta ggttgatatc
    50041 atgtgtcgca actgcgtccc cttcatggaa gatttcgatt ttaaatccag cacacggatg
    50101 gatttcaaca aattcactgc cattgattcg tccaaaactt gttgtgattt gtttgatcca
    50161 aagatcgccg atatgacggc ggttgatcgt ccaggtttct ccatttctaa aataaaattt
    50221 tagcgctgtc atttctttac tcattaaaaa gactccttta tgatgtaatt tattcttgta
    50281 ctacctcagt ttagcacaaa tttatgtaag tggtcacatc ttatgttttt tcttctaatg
    50341 acgtatctgc ccactttaag tagccaacaa agctgagcag atacagtcta gtgtttttta
    50401 aatcaatttt tctttcgata aaaaatccaa aacctgtttt ttatcatttt ttattctttg
    50461 ttctacgacg gctttttcag ctaattgcaa agcttcacta acccacttac caggtttttt
    50521 tccagtttct tctagcaaga ttttaccatc tactgctaat tcgcttaatg acttgatagg
    50581 taaacgatca taggattctc ctacggcatc catctgaggt gtttcgtcaa aatagatcat
    50641 caattcttct acttgcagtg catgtttttt gcctaagaag tacaattcga atggttccaa
    50701 agctccagtt tctcgttgtc gtagtccttg gacaagtgct tgaacgtctt taatcatttg
    50761 gttagaacac ttccacgatt tcaaaaagga acgaatctca ttttctttta gacccagttg
    50821 gctgatcaat aatgtccata cttggctctc ttctttcaat gggacggatg gtaattcagc
    50881 aaaacgtaaa agtgcttcgc cttcttcttt caaactggga cagcaaatgt agcactctgt
    50941 ttcaataaat gcatggattc ccgcggcccg ttcatttccc aagagcatct tgatgaattc
    51001 cacatttata cgttctacag atattttacc taatagctcg tggttttctt tgatggcgta
    51061 gaaggtttct tcctctaatt tgaaccctag ttgactgaca aaacgcaacc cgcgcatcat
    51121 tcgtaaggca tcttcatgga accgctcatg aggattgcct acagctcgca agatctgttc
    51181 ttctaaatct tgcagaccat cgaacaaatc gatgatttct ccatcttctt tcaaagcaaa
    51241 cgcattgatc gtaaaatctc ttcttttcaa atcttctgcc aacgaacgaa cgaattcgac
    51301 atgatctggt ctgcggtagt cctgataagt tgattccgtc cgaaatgtgg tgatctcata
    51361 ttgttcatca ttttctaata ccaacacagt tccatgttcg atcccaatat ctatcgtttt
    51421 tggaaaaagc tgtttgattt cttctggaaa tgcgcttgtt gcgatatcca catcatggat
    51481 tggctggttc agcaagatat cacgtacact tccacctaca aaataagctt catatcctgc
    51541 attctctaat tttttcagaa ctggaattgc cttttggtat tccagaggta attgttttaa
    51601 tttcattttt ccaacctttc ctgcgtgttt cttgtactga gataaagact cagttgcatg
    51661 gtgactgttt gacaagtccg tttcagagtc ttcaaggcca gaaccacttc atcttgttta
    51721 ctcgtcctta cgctcaaagc gattatggtc tctttgattg aatttttcca tgtttttttc
    51781 aaaaacttca gttaagtcga tattcaatga attagccatg atcatcgtaa cgaataaaac
    51841 atcaccaagt tcttctgcta ccgagttaac tggttcagaa gcctttttgc ttttttctcc
    51901 ataagtgtgg ttgacttctc ttgccaattc acctacttct tcagttaatc gagccatctg
    51961 agctaatgga gagaaatacc ccaccttgaa ttgctggata tattcatcca cttcctgctg
    52021 catactgtat aaagaacgct ttttatccat ctttttccct ccaaaactat cttaccaaaa
    52081 ctttaacgca aatcctagat aagagcagtt gagaaaatcg aatttccatg ctacatttag
    52141 aaaggaattt ctaaatggaa attctgtgaa aatacataat tgaaagctgg aattcatatg
    52201 gtcaaacgaa aaacagtaaa agaagttctt tttatcatta taggaacaag tatttatgca
    52261 tttggtcttg tttacttgaa catcgctaat catttagcag aagggggcgt ctcagggatc
    52321 accttgatct taagagcttt attcggaatt gatccagctt atacgacttt attaatcaac
    52381 atcccattga ttttactagg aggaaagatc ctcggtaaac gttccttggc ctatacagtt
    52441 ctaggtacac tttctttgtc attcttttta tggatctggc aaagggttcc actagaaatc
    52501 aatttacagc atgatctact gatcgtttct ctacttgcgg gtttagttgc aggtgtagga
    52561 agcgggatcg tctaccgcat gggtggaaca acaggtggca gcgatattat tgctcggatt
    52621 ttagaacaaa attacggcat cactatgggg cgttccctat tggcatttga tatcatcgtc
    52681 ttacttgctt cattaaccta tatcgacttg aaacgtatga tgtatacgtt gattgtttcg
    52741 tatgttttca gtcgtgtgat tgattcgatc ttagatggcg gatattctgc aaaaggtcta
    52801 cttgtcgtct ccaacaaaag cgaagagatt gcaccactac tgatgaccgg tctgcaaaga
    52861 ggtgtgactt ttctttctgg agaaggcgca ttctctggta attcaaaaaa aatcatctat
    52921 atggtcgtta gctctagtga attaactgaa gctaagagga tcgtccacga gattgacgaa
    52981 aaagctttcc tttcaatcat caatgtgcat gaagtggaag ggcaaggctt tacttatttg
    53041 aagccacaga caagattact gaaaaaacta taagaaaaaa gacaaagata ggctatgagg
    53101 aaagattcca catccagctg atctttgtct tttttagtac tatactattt ttaactaatt
    53161 gttatgttca cttacccggt tacttttctt ttgtgtctgc aaaaggatct ttcggttaac
    53221 ttcatcagat aatttcaaaa aagcatccct atctccatct tctagtgctt gatcgatttg
    53281 agcataaagc atttttagtt ttgcctcttc ttctttttct tgaaaatatc gttcaattaa
    53341 ttcagtatct tcttcactga ctgaagaatc ccaagaagaa aatggattgt cttctaagat
    53401 agatagatat tctcttgtct gccaagcatt ttcgaatata cattccacat ataaaggctt
    53461 tttccaattc aaacggattt catgaaaaat ctggtctgtg tccgtaaatt cttttccctg
    53521 caaaaacaat ttcatgggtt ccccttcaac agatagatca ctgatctgca gtcctcgatc
    53581 cgcaaaattc gccccttcaa caaagtggac attgcttaaa atcgcttcat gattggcaag
    53641 ataattcaag atccaaaaaa cttctcttcg gctaaaagat acatgattga ccatccacgt
    53701 taaaaaattc ttcttttccc gtacatcaat tagcattttt ctcacctctc tgttaggtca
    53761 tctaataatg acagtgcctc catatctcct ggttcaagtg ttaaataata agtcaataat
    53821 tcttgcgctc ttttcagctg cccttcttct cttaagaaaa aggcatactc tttcaagaaa
    53881 tctggttcat gtgatagttc atgataagcc tgttcatagt gtacagctgc aacggtaaag
    53941 tcttctaatt cattataagc atgggctagg ttccattctg cataaggatt agcagtttct
    54001 tccatctgat tgatcacttg tatcacatct tcatatcttt catcatccaa gtaaagattg
    54061 cttaaagtaa gtaatgtttc atcttgtttg tctcctactt ctaaagcttt cagtaaaagt
    54121 tcttctgctc gctgtctatc atgcagacgg aaagcatttt ccgaggccag gtgataaagt
    54181 tcgacttggt aaggattttc tttgattcct tcttctaaca ctgtttgtgc ttcttcaatc
    54241 atttcttctt cttgaagagc ttgtcctaga tatagatata acgattgata atgcggatta
    54301 acttctcgaa gttcttccag atagttgatt gcctgctgat tttctttcaa ttgaagattc
    54361 gtaaaagcta gctggaacag tcgatcatct gtcctttctt ctttcagtgc ttcttctaaa
    54421 ggtgcgatag ctgcttcgaa ttctccttgc atacttaagg attgtcctaa acgttcctgc
    54481 atcgagattc ctgaaatttc atttatcccg cttgccaaca atgtttcata aaccactgct
    54541 gcctctttga aacgatcaac tgaaaaatac aattcagcta gcgcaaactg gatcaatggt
    54601 tcatcaggaa gcaacccagc tgcttctttc aattttgctt cactcacttc agggattccg
    54661 ataacttggt acaaatcagc tatcgccaat aagctttgcg gatagtaatc actttgctta
    54721 tccacttttt ccaggtattc aaaagctaag tcgatctcat tatcttcgat tgcaatttct
    54781 gctaacggga tgttcagtcc gtcattttct gggtattggt tgatcagttg ttcaaaaatc
    54841 tgttttgctt cgatcaaaaa gccaatagcc agcaattctt cgcctagttc agctaatata
    54901 tctgctgggt cttcttttaa tgctttttct aaaaagattt gtgcttgtga caagtcttca
    54961 tctgataaag cttgcaacat tttttcgcta tttgtattca tttcaattcc ctcttatctc
    55021 actgacgatt tgttgaaata ccgtcattgt tctcaataat tctttttcgt ttggacgttg
    55081 gtatccgtct attataccta ttttttctaa aacaagtatt tttttgcttt tttctgccaa
    55141 aaaaagaact ttttccaaat actccatctt tgaaatctgt tccagccagt ggattggata
    55201 gccaagcaag tccagccaca caaaaaagtt ttttagttgg aaatcaaaag gatatgcttg
    55261 gagattccac acgaaatgaa acatcattcc taaaaatttt ttcatactag cagaaagtag
    55321 atggccattt tcggtcagat aaaaagcttg ttcaaacagt ttaccataat ttgacaggtt
    55381 ttctgcctct aattgataaa aatagatcat ttcttcgatc atcccggcaa acggccgagt
    55441 cgttaattgc tttttatttg gatagttttt atacagattt tgcaaaaaac gatgatcaca
    55501 aatcattccg caacagataa aagtcaaaaa gtcaatctgc tttccgtcca cttgccgctc
    55561 gctgattgtt tgatcataaa cgatgcgttc tggcagattg atggtttgca gtactacacg
    55621 atttggtagc tgcaaaatcg tccgctgcgg tgttaatgct cggcttagag aacggatcga
    55681 tacagggatc atccatagtg atgaatcaag ggttgtatgt ttttgaaaaa aacctgccag
    55741 atccatgatc ccttcatttc caaatccgat gataaacatg gattgatttt cgggatagcg
    55801 tttgacgaaa tcaagcaggt tctttaattc tgttaaatga ttgcattgag tgttggcaca
    55861 aatataccag tcattgtttg cttgatttgc aaataacggg ttgatcttat ctgcaaacaa
    55921 atcataatag cgctgattcg ttaaaaataa gattggctga tcaatggaaa gttctcttag
    55981 ctggctgact agcgtttctc cgtaaaacac tttcgtcaac gcatcttcta cttgataatc
    56041 aagttccata cgcatccctc ctcttcttat ttttatttta ccattagtaa gcaaattcta
    56101 gaaaagataa gagcaaagct cacctcataa cttggaaata aataaaaatt aaataaaata
    56161 catgtgtttt ctatgtatga catacggatt cggaatctct gtctcttatt tattcctttt
    56221 ctgctaggaa ataaaaagaa aagcacttgt agccctagag ccacaagcgc tttttcaact
    56281 gtcttattta acagcgtctt ttaatgcttt acctggtttg aatgcaggta ctttgctagc
    56341 tggaatttcg atttcttcac cagtttgtgg gttgcgacct ttgcgagctg cgcgttcgcg
    56401 aacttcaaag ttaccaaaac cgattaattg aactttttcg ccgttagcta aagcatcttg
    56461 aatagttgaa aatacagcat ctactgctgc agttgcatct tttttagtta aatcagtagc
    56521 tgaagcaact ttttcgatta attctgcttt gtttgccatg gatagattca cctcctccac
    56581 aagagatatg atagaaatat catttagtta aaactttctg agtggtccag aagtttaaaa
    56641 agaaatattg attaacttca atattctgtt atcacgatat catatgaacc gcttaatagc
    56701 aaggttttta ccaattttca catacttttt ttagtcatag aaacgtaaca aacgaaactg
    56761 tctttttctt ttgaaaaaaa aacgtcgata tggtaaaatg cctattttct cctacgaggg
    56821 ataattcgga tggacgtccc ttcaaaagta aatgcttttc tgatttgatt ttctaagaaa
    56881 cgttcataag aaaaatgcat caattcctct tcattgacaa aaacgacaaa tgtaggtggt
    56941 ttgacagcga cttgtgtagc ataaaaaatc ttcaggcgtt ttcctttatc tgttggtgtt
    57001 ggattgattg ctacagcatc cataatgata tcattcaaca cagccgaaga gatacgaaga
    57061 ttctggttca tacttacttg ttcgatcatt tcaggcagac gttccaaccg ttgtttagtc
    57121 actgcagaaa caaaaacgat tggagcgtaa tcaagatacc ggaattcatc acgaatctct
    57181 tgttcgaaat cacgcattgt attggtttct ttctttacaa gatcccattt attcacgacg
    57241 ataataattc cacgaccagc ttcatgggcg taacccgcta ctcgcttgtc ttgttcccga
    57301 atcccttctt cagcattcag taccatcaaa acgatatcag aacggtcaat ggcacgcatt
    57361 gcacgcatca cgctgtattt ttcagtattt tcgtatactt ttccgcgttt acgcatacca
    57421 gctgtatcga tcattaagaa tttctgacca ctttcagaaa cgaagtgggt gtcgattgcg
    57481 tctcgggtcg ttccttcaat atccgaaaca attacacgtt cttctcctaa aatcgcattg
    57541 atcaaagagg atttaccaac atttgggcga ccaatcaaac taaacttaat cgtgtcgtcg
    57601 tcctcttctt cagtctcttc agagaaatac ttcactgctt catctaaaat atcaccaatc
    57661 cctagtccat ggcttcctga aacaggataa ggatcgccta gccctaagga ataaaattca
    57721 tagatatcgt ttctcatttc tggattatct actttattga ctgctaaaat cactggttta
    57781 ttacttctgt ataaaatttt cgcaaccatc tcatctgcgt ctgtcacgcc ttcgcgtccg
    57841 ctgactacac aaatgattac atctgcttct tcaatcgcga tttctgcttg atgcttgatc
    57901 tgatccataa agggttcatc gccaagatca attccgcctg tgtcgatcac gctgaattct
    57961 cgacctaacc attcaccttt tgcataaata cgatcacgag ttactccagg agtatcttcg
    58021 acaatcgaga tacgctcacc agcgatgcgg ttaaaaatcg tagacttacc gacgttcggg
    58081 cggccaacga ttgcaattgt tggatttgcc atattatttc ctcctcacaa gtttccaatc
    58141 tcacttagtt taacgaatgc attgccaact ttcaagcata gattgtcaat taaacgaagt
    58201 atttttattc cttattgaaa tactaatcgc ttttcaaaag aaaaactgag acaaaagcag
    58261 ctgctttcat ctcagtcaca gatacaaaat caattatttt tcagaatcat cggttgcttt
    58321 ttcttctact tcttctttca acgcatcacc taaaatgtct cccatagtaa atccagtatt
    58381 ttcttctggt aattcataag actcaacatt ttttggttct tcttctgaag ctggtttttc
    58441 ttctaaagct ttgatactca atgcaatacg atgttcttct ggatggatct ctaatacttt
    58501 cactttcact tcgtcacctt catgaagcac ttcatgtggt gtcgcaatat gtttatgtga
    58561 aatttgagag atatggacta gcccttctac tccagggaac acttcaacaa aggcaccgaa
    58621 gcttgtcaaa cgtttaactg ttccgtctaa tactgtgcca actggagctt ttgtatcgat
    58681 ttctgaccaa ggtccaggct gtgtgtcttt gatggacaag gaaacacgtt cttgttcagg
    58741 attgatagac aacactttga catttacatc atcattgaca gccaatacat cacttggttt
    58801 tgctacatga ctatgtgaaa tctctgagac atggactaga ccgtcaattc cgcctaggtc
    58861 gacaaatgcg ccaaaatcag taagacgtgc aacacgtcca ctgagaacat ctccctcatg
    58921 gatagttgaa agaatttctt ttttcttttc ttctctttcg ttttccacta ccgctttatg
    58981 cgataagatc aaacgatttt ctgatggttc gatttcgatg attttaaagc tcaatgtttt
    59041 gcctttatag tctgaaaagt ctgcaacaaa atgatcttcc accattgatg cagggacaaa
    59101 accacgtaca ccgacgtcaa cgactaaacc gcctttgaca acatttgtaa caggtgcttc
    59161 gatcaccttg ccctcttgga aatctctttc gatatcttcc caaacttttt tagcatctaa
    59221 acggcgtttt gaaagtaggt agctgccgtt ttctttatct ttgccgatcg tactgataac
    59281 gactaagtct aatacatcac cgacttttac tacttcgctg atatcttcta ccggcaaagt
    59341 tgataattct tttgccggaa cgactccttc aactccagcc ccttcgattc cgacaatcac
    59401 ttgtttgtct tcaatgacta aaacttcgcc cttaacaaca tcgccaactt ttacttcttg
    59461 tacgcttttc attgcgtctt ccattgactg gttgttctct tgattttgat caaattctgt
    59521 cataacactt gtcctcctaa ccaacattct gccgtaagta cggtactgtt tttagtttaa
    59581 ttgattattc aaaaaaaata aagcgatttc gttcgttttc cttgaaaaaa caattgtttt
    59641 tcattttttt tcttaattta aaaaagaaga taaccttttt tcaaaacaac agcttttatt
    59701 gctgcaacga cttctggaat cgatttgcca gtcgtatcga tacgaacagc atcctctgct
    59761 tgcttcagcg gagagacgtc ccttgttgaa tcataatggt ctctttcctg tatctctttc
    59821 gtcagtgttt caagatcagt cggaattcct ttttcttggt tctctttgaa acgtctttct
    59881 gcacgctcac ttacgcttgc tactaaaaag attttcactt ctgcattcgg taatacagct
    59941 gtcccgatgt ctcttccgtc catgacaacg ccaccgcgtt cccctatttt tcgttggata
    60001 gcaactaatt cttctctgac ttttccatgt gcagagactt cagaaacagc ttttgtgaca
    60061 tctggttgac ggatttctaa agtcacatct tttgatgcta tgaatacatg ctgtccatct
    60121 tcttgttgcg aaaaagagat aggatacttt ttgatcagcg aaactagccc ttcttcatct
    60181 gaaaaggata cattgttcgt aatagctaaa taagttactg cgcgatacat ggcaccggta
    60241 tctgtataaa tataattcaa ttcttttgct aatatcttgg caacggtact tttcccagat
    60301 gaagcaggcc cgtcgattgc aatactgatt tgtgtcatga tataactcct ttttctcaaa
    60361 aaaaagccgc aaagcgcggc tattattatt taattcttaa ttcttgacca gggtaaagca
    60421 taaagttgtt tggatctaag ccattcaatt caaacaattg atcagtggtg atccctgcac
    60481 gctcagccac ttgttttgga ccttcgcctg cttgaacagt cgttgttgaa ccggctgcag
    60541 ctgatgaaga aggtgttgtt tctgctgcat tgctgcttga atcccctgct tgataagttg
    60601 cctcacttga ggaagtcggt gttgtctctg ctgcaatact gctttctgac gttacacctg
    60661 tatcttgaga cacagtagat tcttccgcga ttgtactaga ttctactgtt gaagtctcag
    60721 ttgactcctc ttttgttgta cttgtagaac tttctactac tgatgtcgaa gtttcagaag
    60781 aagttgctgc tgttgcattg ttgcttgatc catttctgat ccagaaataa gcacctgctg
    60841 gaatagcgat acataagacc agtaaaatca aaaatagcga aagaaataat gtatttcctt
    60901 tcttttgttg tctctgagaa cttcttgatg aaacttcatc atctgtatcg tagattggtt
    60961 gttcccatgg ttcttgtgtt tcgttatttt ctgtagattg atgtctatct tttctgctca
    61021 ctgttttacc tcctaaactc tatcgtgtgt attataccat aggaaaaagt gtcttgtcct
    61081 aaaaaatcaa tgttccatgt tttttttaaa taaatttagt aaaatcgttt cccaatgaag
    61141 ttgttcagtc ttagaaaaaa gcttcctctt tggcagaagt tctgcttttg ctccatcaat
    61201 atcacaacaa ttctgcggct tttcggagag tttttcgcca aaataagcta agatgaattt
    61261 tctgcggcag ttttcttcat taatatagcg aagcatctgc tgcaggcgga attctttttc
    61321 tttttcctga tgtttgagcc gttccaccca attttctggt ttttcagact gctggatcag
    61381 ctccatccat tttttttgca gctcatcaaa gggagcttgc tctgtggcat attccaaata
    61441 aagctcaaaa ctttgtcgct gttctctaga aagttgatta aagaaataat ggatacgttc
    61501 atccccactt tgataaaaaa gaattgctgc actttcttcc tgatctctgc ctgctcgacc
    61561 gatttcttgt acatagtttt ctagactgtc tggcaaatca taatggatca cataacggat
    61621 atcagattta tcgatcccca taccaaatgc attcgtagca atgagaaact gcagctgatt
    61681 ttttacaaac tgctgttgaa gttgtcttct ttgagctgca tcaagaccac cgtgatagta
    61741 tccaactgta aaacgttccc tgaataaatg atacaatcgt tcgacttctt tttttgttgc
    61801 acaataaatg attgccgctc cttgtgctct ttccatgaag tgttcaagtt cttgttcttt
    61861 ttgctgcgtt ttccgaacaa aaagcgagat attttgccga ttgactgatt gtctgatctc
    61921 ttttggtggt cgatcaaata gtacatgacg tatatctctt gctactaaat ctgttgctgt
    61981 cgccgttagt gccagtgtga ccgggtttcc taaaagcttt cggatttttc ctaactgttg
    62041 atattctgga cggaaatcaa ctccccattg agacacgcaa tgagcctcat ctactacata
    62101 taacgcaata ttttgttttt tcagatgctc taaaagagaa ggattagtca acatttcggg
    62161 actcaaaaac aaaaatttat attgcgatag atgacggagg acgtatcttc tttcagattg
    62221 cgaaagtaaa ctattgaaag cagctactcg tttctcccct cgtttttgta attgcgtcac
    62281 ttgatcttcc attagagaaa gcagcggtga cacgatgatg accagacctt ccattaaaaa
    62341 ccccgtcaat tgatagcaaa ggctttttcc cgttcctgta ggcaaaatag cgagagtatc
    62401 ttctccatca agcaacgcct gaatgatttc ttcttggccg ggacgaaatg aatcaaatcc
    62461 aaaatatttt tttaattcat ttattaacat tctgtatcgc ttcctttctc aatagataga
    62521 tttgatacaa acgaaattct ccataatcta attgtccatc tacattccat tcgctaaagc
    62581 gccactctcg cacggaatcc ttatttggtt ccaaacgttc gattgtttct gcagagagaa
    62641 tccgctcgta tggaaaatca tcaaaaaaca aggcccactc aataagatga tctgttactg
    62701 ttcctctttt taaatgacgg attgccataa tctggtcaat tgtctgacca ttcataaaca
    62761 tctgcttggt gatcatcatg ctttgattga aattttgctg taataatgaa tcgatcaaag
    62821 cgtgccaaag cgaatctggc cgtttttcga tttgtgctaa caaaaggtgg atacatctgc
    62881 tttgttgcag ataaaccgct gactcatcat tgttttttgc cagctgataa ggaagcagcc
    62941 ctgttcgctc atttccagat aattggttag ctaaaaaatc tgctgcttct tctggtatct
    63001 ccgaaaacag ctgcaccaaa ctctcatacg cagtatcaat caaaatttca cgtggcaatt
    63061 ggctttcact aagccatttt tttagttgga aagtataaaa aggacttgtc tctgctggaa
    63121 gatagtcttg aatacccgtt gccagattag aaattacttg aacagcaaat ttaatgagcc
    63181 gccaactagt ttcccctgtt ctgccatagc ggtcaaaacg caaaccagta tgttctattt
    63241 gatgttcgga caacatatca gcccctgcag gcgttagctt tgcttcattt tcatttattt
    63301 cgatccaacc ttgctgcgca attttttgca aaatttgata aaacttatcc tgcttcaatt
    63361 ctggaaaact gttatggata aacaaaagtt cgtgggtaaa tccataaata agtactgaag
    63421 tcgttcgttt tcccacaagg agttggtaaa gagaactaat ccgcagcttg tgtcctgatg
    63481 aaaatagcga taaaataaaa acaatcatat tattctcctt aagttaggat ggttagaaat
    63541 gtcactacta tgtaaacttg tacctgaacg ctgtattgca tgcggtcttt gtcaaatcga
    63601 agcgccagat attttcgatt atgatgaaga aggaatcgtt ttatttgccc aagaaccaga
    63661 ggctcatcaa cagttcgtga tgccggaaga agaaacatat gtaaaaaaag cgtatcaaaa
    63721 atgtccagtt cgagcgattt tattagaaaa taaaatataa atataaacga aactttaaaa
    63781 acgtacacta agttctctaa acttgaaaat aagccgggat ctgtttctct tgttccgtga
    63841 aatcccgact tattttgaaa gattcatttg ctatctatag ttaattgact agttttttca
    63901 cggaccctat aggacccatt tatattattt caactttttc agccagtttt ccaatgtatt
    63961 gacgatcaca gtttgctggt ctgcgttaga aatttctgcc tctccatctc ctttttgctc
    64021 accatagctg ccaaaacctc catgatttcc gccattgatt gatacatact ctgttctttc
    64081 cggtaaatac tgcttagctt cttgataagc ttcctgattc aatactttat cttttgtcgc
    64141 cgtgatcgat aaaacaggaa cagctgattc tgtcaatgtt ccttttttgt cgggataact
    64201 tgccatgaag aatacccctt ttaaatgttt gtctgaatga ttcttattga cataacgact
    64261 tgccattacg ccacctaatg aatggccacc gatcacataa tcggaatttg gaaaatcttc
    64321 tttgacctta tccgctttgt ttccgcccat cactgctaga tcaagtggca ttttgacaat
    64381 agcgactgaa aagccgtttt ttgctaaatc ttgcgcccaa atactataac tttctggctc
    64441 tacgagagca ccaggataaa aaataagtaa tggagaattt ccatgattct ttttctctcc
    64501 ttcaaaaaat aagtaatctt ctttgttttt tccttctttt accaacactt gcgcttctga
    64561 cgatggttcg tacgtatttt gctgtatata gtaataaccg gctccaccga gtaatcctaa
    64621 aatgatgact aagccaatta gtattcgtct aaatattttc actttctccc accttttcta
    64681 tctgagtgac ccctttttct taatcaagta tgattatacc ttaaaaaaaa taagagagaa
    64741 atgcaaactt tccagcatca aataaaaaaa gacggaatat ccatttgaat atccgccctt
    64801 tttgtatgtt tatttttatt tagcacataa acgatacaca gcttctgcat aaatttccat
    64861 tgctttatac atactactta attcccatcg ttcattcgcc tggtgcataa agtcaggagt
    64921 atcagggaac atcgcaccga atgccacaca ctggttcatc gtacgagcaa aagtagcgcc
    64981 gcctgataca aacggttcag tcatgtctcc agtaatatcg cgatatgtcc ccaatagatt
    65041 tttcactaac tcactgtcta gtggcacgta taatgaatca aggaagtcaa attcttcata
    65101 agtcaaccca tatttttctg ctgttttagt taatttagct actaaatcat ctttcttgaa
    65161 tgtaacaggg attctcatat ctacaccgat ttttgtttgt tctggtgtga tttccaaact
    65221 tgcaaagttc atcgttagct cgccggattg ttcatcttct gttttgccca cgacagcttc
    65281 accagttgca ttttcttgaa ctaattttcc taagaaattg attgggccaa aatcgaatac
    65341 ttctgaaaga gcaattgcca gacgagaaat agcattcact ccttgtgctg catctttcgc
    65401 gtggatactt ttaccaagaa cgacgatgcc ttcaccttgt tcttcgaaat cgaattcgtg
    65461 ttttttcaac gcttctttta cttcagaaag cttttctcca gagtaaggag ctgcatcagg
    65521 aacaacattc aacgctcctc cagcttttac agaaaactcg ttcgttcctg gtcctgtcaa
    65581 gtaagcttgc aataagcctt tttctgcata aattaaagga aattcagcat ctggcgcaaa
    65641 tccttgcgta attccttctt ctttttcgtt atatttttct aaacaacgcc ataaattttc
    65701 ttcatctgta ccaaagataa aacgaatacg tgtcttgaat tccacgccag catcaatcaa
    65761 agctttgacg gcatacattg ccgcaataga aggacctttg tcatcttggg atccgcgacc
    65821 aactaaccag ccgtctttta tagttggatc gaatggtttt gtttcccaat tattttcgtc
    65881 ccctgctgga acaacgtcca tatgacacaa gataccgaat aattcatctc cagagccaat
    65941 ctctgaataa ccgtaatagc cttctggatc tttaaatgtt ctaaatccta gcttttcact
    66001 gatttccaat actttatcca aagcttcaat cactttttta ccgaagggat gtccttgtcc
    66061 tgaatctgct tcatctagta cggaaggaat tcggatcaat tcgctcaatg attctaatgc
    66121 ttgttcatga tgttgttctg tcacaaattt tttcatgata ttatcctcct aatatgttaa
    66181 aaagggtcat gtcaaattcc tccgacacaa cctcttcttc tcaaataatg acgttttaag
    66241 cctgctccat cagcaagcag aatgttctta acaaatgatg aaagaaaaaa cactcactga
    66301 atggagctat tgacttgtcc acgtgcttat cgatagccgt ttatcggttt cttcattctt
    66361 atgatagacc ttgtacctgt atttgtctag gtttagaaca ttgctgctac acctaggatc
    66421 acacagatgg ctacaaataa tatcgcaatt aatttaccgg taaatttcca ccacactgaa
    66481 acatcgaaac gagcaattgc tacagcaccc attacgatag cagatgttgg tgtaattaag
    66541 tttactaaac cagatgcaga ttgataagca gtgattacta aatccttacc gacgccggca
    66601 aattcaccca taggtcccat gattcccatt gttgcagcag ctaagccgga agttgaagga
    66661 atcaagaatg acatagggat atagaaaata aatgtaataa tgatgaatac acttgaagat
    66721 aatgaactta atccttgttc gccccaatgt aagatggtgt ctgtgattaa tccattgttc
    66781 ataaccactt ggataccacg agcaacagct actacgattg ctacaccgat caaatcagaa
    66841 gcaccagcta aaaattcaga gacaaattca gactctttca tgccataagt gattgctact
    66901 acgatggaca ttactaagaa taacatagta atttctggga agtaccagtc acctaatgga
    66961 agcatgcttt tacctagaac agtacctagt accggaaggt ttgttaacca gtttgtcaat
    67021 gattcaaaaa tcgtgaaatt cgcattcaag gttgtccaag gaatcaaact gatgatcatg
    67081 ataccaaaag taataaagaa caatacatta acatgttttt gttttttcgt gattacttct
    67141 tgacgaccga ttgcttcaat atcgaaatgc ttcatatctt ctttacgttg gttgtatact
    67201 aatgattttg ttggatcttt ttcgatcttt gatgcataac gatacacata tacaatagaa
    67261 atagctaaca aaataacgaa catcaagaat cgaagaccaa taccttcacc catcgaaatc
    67321 ccaacagctt gtgaagctac gcctgttgca aaaggattta ccgttgaagc tagacagcct
    67381 acttgagacc ctactaatac gattgctact gctactactg tatcaaagcc tacagccatc
    67441 ataactggga tcagtaaagc atagaatgga atcgtttctt ctgccattcc gtaagtagag
    67501 ccgcctaatg caaataacag catcaatact ggaataagca ttttttcttt tcctttgaat
    67561 ttcttcacaa ctgaagcaat cccggcatct agtgcccctg ttttgttgat aatacctaaa
    67621 aatccaccaa caaccaaaat gaagaaagag attgggattg cagcaggtgt ttctttagtt
    67681 agctcccccg ctggtacacc taacatccca ttgattggcg ccatgaaaat atcccacagc
    67741 ccttgaggat tttgttgtac tcgttcatat gatccagcga cgatgttgcc tgcatcgtct
    67801 acactatagt gcccagctgg tataaaccaa gtaagtacag cgattactgc aataataata
    67861 aataagaccg tatacgctga aggcatttga aattttttct tttttgtttg ttccatcttc
    67921 ttcttccttt cttttccgtt agagacaaca attttggctc taatgttatt ttatcaaaaa
    67981 gagtatcaga acaaaaataa taacacgcat tttgtaaaac ttttgttcaa aaagttcgtt
    68041 tttccctatt ccctcctctt ttttcaggtt tttaaagctt tcttcttttt cattgtagcg
    68101 tttccttttt tttataaaca aacattttcg tcttttaaaa ggagaagaat ttcacatttg
    68161 caaagaattg attgattttt atgcataaat caaaaaaata ctc
//
LOCUS       DAZUKC010000006        60934 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000006 DAZUKC010000000
VERSION     DAZUKC010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.006"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..716
                     /gene="purC"
                     /locus_tag="ACG80T_000341"
     CDS             <1..716
                     /gene="purC"
                     /locus_tag="ACG80T_000341"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFS4840025.1"
                     /translation="LLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQISGKGEL
                     NNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFSKRLGV
                     PEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINEILTEL
                     FAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDLGDLIP
                     VYQEVYNRLSELTEEKERV"
     gene            716..970
                     /gene="purS"
                     /locus_tag="ACG80T_000342"
     CDS             716..970
                     /gene="purS"
                     /locus_tag="ACG80T_000342"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFS4840026.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            972..1643
                     /gene="purQ"
                     /locus_tag="ACG80T_000343"
     CDS             972..1643
                     /gene="purQ"
                     /locus_tag="ACG80T_000343"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFS4840027.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            1644..3866
                     /gene="purL"
                     /locus_tag="ACG80T_000344"
     CDS             1644..3866
                     /gene="purL"
                     /locus_tag="ACG80T_000344"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFS4840028.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            3851..5290
                     /gene="purF"
                     /locus_tag="ACG80T_000345"
     CDS             3851..5290
                     /gene="purF"
                     /locus_tag="ACG80T_000345"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFS4840029.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            5313..6365
                     /gene="purM"
                     /locus_tag="ACG80T_000346"
     CDS             5313..6365
                     /gene="purM"
                     /locus_tag="ACG80T_000346"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFS4840030.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            6362..6940
                     /gene="purN"
                     /locus_tag="ACG80T_000347"
     CDS             6362..6940
                     /gene="purN"
                     /locus_tag="ACG80T_000347"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFS4840031.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            6937..8472
                     /gene="purH"
                     /locus_tag="ACG80T_000348"
     CDS             6937..8472
                     /gene="purH"
                     /locus_tag="ACG80T_000348"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFS4840032.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            8488..9762
                     /gene="purD"
                     /locus_tag="ACG80T_000349"
     CDS             8488..9762
                     /gene="purD"
                     /locus_tag="ACG80T_000349"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFS4840033.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLTGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            9884..10798
                     /locus_tag="ACG80T_000350"
     CDS             9884..10798
                     /locus_tag="ACG80T_000350"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFS4840034.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(10931..12496)
                     /locus_tag="ACG80T_000351"
     CDS             complement(10931..12496)
                     /locus_tag="ACG80T_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4840035.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(12489..13940)
                     /locus_tag="ACG80T_000352"
     CDS             complement(12489..13940)
                     /locus_tag="ACG80T_000352"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4840036.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIRESKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(13954..14970)
                     /locus_tag="ACG80T_000353"
     CDS             complement(13954..14970)
                     /locus_tag="ACG80T_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4840037.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15190..16278
                     /gene="chvE"
                     /locus_tag="ACG80T_000354"
     CDS             15190..16278
                     /gene="chvE"
                     /locus_tag="ACG80T_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS4840038.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            16332..17858
                     /gene="mmsA"
                     /locus_tag="ACG80T_000355"
     CDS             16332..17858
                     /gene="mmsA"
                     /locus_tag="ACG80T_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS4840039.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            17860..19065
                     /gene="mmsB"
                     /locus_tag="ACG80T_000356"
     CDS             17860..19065
                     /gene="mmsB"
                     /locus_tag="ACG80T_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFS4840040.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            19322..20299
                     /gene="guaC"
                     /locus_tag="ACG80T_000357"
     CDS             19322..20299
                     /gene="guaC"
                     /locus_tag="ACG80T_000357"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFS4840041.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            20563..21987
                     /locus_tag="ACG80T_000358"
     CDS             20563..21987
                     /locus_tag="ACG80T_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFS4840042.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(22029..22286)
                     /locus_tag="ACG80T_000359"
     CDS             complement(22029..22286)
                     /locus_tag="ACG80T_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840043.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            22448..23557
                     /gene="pdhA"
                     /locus_tag="ACG80T_000360"
     CDS             22448..23557
                     /gene="pdhA"
                     /locus_tag="ACG80T_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFS4840044.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            23560..24537
                     /locus_tag="ACG80T_000361"
     CDS             23560..24537
                     /locus_tag="ACG80T_000361"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFS4840045.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            24562..26205
                     /locus_tag="ACG80T_000362"
     CDS             24562..26205
                     /locus_tag="ACG80T_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFS4840046.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26213..27619
                     /gene="lpdA"
                     /locus_tag="ACG80T_000363"
     CDS             26213..27619
                     /gene="lpdA"
                     /locus_tag="ACG80T_000363"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFS4840047.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEITILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            27781..28056
                     /locus_tag="ACG80T_000364"
     CDS             27781..28056
                     /locus_tag="ACG80T_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFS4840048.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28062..28829
                     /locus_tag="ACG80T_000365"
     CDS             28062..28829
                     /locus_tag="ACG80T_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFS4840049.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            28997..30826
                     /gene="typA"
                     /locus_tag="ACG80T_000366"
     CDS             28997..30826
                     /gene="typA"
                     /locus_tag="ACG80T_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFS4840050.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            31087..32859
                     /gene="recQ"
                     /locus_tag="ACG80T_000367"
     CDS             31087..32859
                     /gene="recQ"
                     /locus_tag="ACG80T_000367"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFS4840051.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            32973..33275
                     /locus_tag="ACG80T_000368"
     CDS             32973..33275
                     /locus_tag="ACG80T_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFS4840052.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            33278..34441
                     /locus_tag="ACG80T_000369"
     CDS             33278..34441
                     /locus_tag="ACG80T_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFS4840053.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            34467..37895
                     /locus_tag="ACG80T_000370"
     CDS             34467..37895
                     /locus_tag="ACG80T_000370"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFS4840054.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISPGDLLIEVTEK"
     gene            37916..39040
                     /locus_tag="ACG80T_000371"
     CDS             37916..39040
                     /locus_tag="ACG80T_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFS4840055.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            39021..39467
                     /locus_tag="ACG80T_000372"
     CDS             39021..39467
                     /locus_tag="ACG80T_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFS4840056.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            39451..39789
                     /locus_tag="ACG80T_000373"
     CDS             39451..39789
                     /locus_tag="ACG80T_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFS4840057.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            39908..40468
                     /gene="rsmD"
                     /locus_tag="ACG80T_000374"
     CDS             39908..40468
                     /gene="rsmD"
                     /locus_tag="ACG80T_000374"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFS4840058.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            40465..40956
                     /gene="coaD"
                     /locus_tag="ACG80T_000375"
     CDS             40465..40956
                     /gene="coaD"
                     /locus_tag="ACG80T_000375"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFS4840059.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            40946..41989
                     /locus_tag="ACG80T_000376"
     CDS             40946..41989
                     /locus_tag="ACG80T_000376"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFS4840060.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            42148..42876
                     /locus_tag="ACG80T_000377"
     CDS             42148..42876
                     /locus_tag="ACG80T_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFS4840061.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            42992..43237
                     /locus_tag="ACG80T_000378"
     CDS             42992..43237
                     /locus_tag="ACG80T_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFS4840062.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            43234..43452
                     /locus_tag="ACG80T_000379"
     CDS             43234..43452
                     /locus_tag="ACG80T_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFS4840063.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            43588..44127
                     /locus_tag="ACG80T_000380"
     CDS             43588..44127
                     /locus_tag="ACG80T_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFS4840064.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            44120..46393
                     /locus_tag="ACG80T_000381"
     CDS             44120..46393
                     /locus_tag="ACG80T_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFS4840065.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            46431..47477
                     /gene="holA"
                     /locus_tag="ACG80T_000382"
     CDS             46431..47477
                     /gene="holA"
                     /locus_tag="ACG80T_000382"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFS4840066.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(47566..47820)
                     /gene="rpsT"
                     /locus_tag="ACG80T_000383"
     CDS             complement(47566..47820)
                     /gene="rpsT"
                     /locus_tag="ACG80T_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFS4840067.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(48210..48488)
                     /locus_tag="ACG80T_000384"
     CDS             complement(48210..48488)
                     /locus_tag="ACG80T_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840068.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPECLY"
     gene            complement(48750..48878)
                     /locus_tag="ACG80T_000385"
     CDS             complement(48750..48878)
                     /locus_tag="ACG80T_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840069.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(48958..49413)
                     /locus_tag="ACG80T_000386"
     CDS             complement(48958..49413)
                     /locus_tag="ACG80T_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFS4840070.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(49551..51479)
                     /locus_tag="ACG80T_000387"
     CDS             complement(49551..51479)
                     /locus_tag="ACG80T_000387"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS4840071.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(51676..52509)
                     /locus_tag="ACG80T_000388"
     CDS             complement(51676..52509)
                     /locus_tag="ACG80T_000388"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFS4840072.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(52701..53405)
                     /locus_tag="ACG80T_000389"
     CDS             complement(52701..53405)
                     /locus_tag="ACG80T_000389"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS4840073.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(53428..54054)
                     /locus_tag="ACG80T_000390"
     CDS             complement(53428..54054)
                     /locus_tag="ACG80T_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS4840074.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            54380..56755
                     /locus_tag="ACG80T_000391"
     CDS             54380..56755
                     /locus_tag="ACG80T_000391"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFS4840075.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(56779..57270)
                     /locus_tag="ACG80T_000392"
     CDS             complement(56779..57270)
                     /locus_tag="ACG80T_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFS4840076.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            57457..58377
                     /locus_tag="ACG80T_000393"
     CDS             57457..58377
                     /locus_tag="ACG80T_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4840077.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            58531..58875
                     /locus_tag="ACG80T_000394"
     CDS             58531..58875
                     /locus_tag="ACG80T_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFS4840078.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(59065..59565)
                     /locus_tag="ACG80T_000395"
     CDS             complement(59065..59565)
                     /locus_tag="ACG80T_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4840079.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            60093..60176
                     /locus_tag="ACG80T_000396"
     tRNA            60093..60176
                     /locus_tag="ACG80T_000396"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:60127..60129,aa:Leu,seq:cag)
     gene            complement(60500..>60934)
                     /locus_tag="ACG80T_000397"
     CDS             complement(60500..>60934)
                     /locus_tag="ACG80T_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFS4840080.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        19981 a        10542 c        13080 g        17331 t
ORIGIN      
        1 aacttttgta tgaaggaaaa gccaaaaaaa tatatcttac ttctgatgag aacttgattt
       61 ggatagaata tttagatcaa gcaactgcgt tgaatggcgc gaggaaagac cagataagtg
      121 gaaaaggaga attgaataat caaatcacaa gtgcaatttt ccgttatctg actcaaagag
      181 gaataaaaaa tcattttatt gaacaattgt ctaaaaccga acaattagca aaaaaagtac
      241 aaattatccc tctggaagtg gttatccgaa atattgcagc aggaagtttc tcaaaacgtc
      301 taggtgttcc cgaaggtaaa gaattcatca aaccaatcat tgaattctac tataaggatg
      361 accagttgga tgatccattt atcaatgaag agcatatcta tttcttacaa cttgcaaagc
      421 cggaagaaat cagtgagctg aaagcccaag cgttggctat taatgaaatt ttgaccgaat
      481 tatttgctaa ggtcggtttg gttttagtag atttcaagct agaatttggc cggtcgaatg
      541 gacaaatttt gcttgcggat gaagtaacac cggatacttg ccgattatgg gatgaacgta
      601 cgaatgaaca tatggacaaa gacgtttacc gcagagatct aggtgatttg atcccagtct
      661 atcaagaagt atataaccgt ctatcggaac taactgaaga aaaggagcga gtatgatgta
      721 tttcgtaaaa gtatatgtaa cctataaaga ttcagtgtta gatcctcaag gggaggcagt
      781 aaaaaaagca gtccatcgta tgggatatga aacaatcgaa gatatccgaa taggaaaata
      841 ctttgagata caacttgctg atgcagaaga accaatcgat acagtgattg aaacgatttg
      901 tgacaagctg cttgcaaatg taaatatgga aacataccga tatgaaatca aaaaaatgga
      961 ggaagtataa catgaaattt gcggtcatcg tttttccagg ttcaaattgt gacgcagata
     1021 tgttatgggc agttcgcgag atcatgggcg ctgatgccga atttgtacgg catgatgagt
     1081 cctctttaga aggatttgat ggggttttac tgccgggagg attctcctat ggtgactatt
     1141 tgagatgcgg tgcgattgct cggttttcta caatcatgga agaagtgatt cgttttgctg
     1201 aagaagggaa accagtattc ggtacatgca atggattcca gatattgaca gaagcaggac
     1261 ttttgccagg tgtactgaga cgaaatgagt ctttgcattt tatttgcaaa actgtaccat
     1321 tgaaagtcaa ccccaagacg gcctttactt cagagtatga agccgacgaa ataatctatc
     1381 ttccagttgc tcatggggaa ggaaattatt tctgtgacga agaaacactt caagaactaa
     1441 aaaaaaataa tcagattgcc tttacttata atgacaacat caacggcagc gtggaaaaca
     1501 ttgcagggat tacgaacaaa gcagggaatg tgttaggaat gatgcctcat ccagaacgtg
     1561 cagtagaaga cttgctagga tcagaagatg ggaaacggtt ctttgcttcc attttaaaaa
     1621 acttcggaaa ggtgaccgta tagatgaaaa cattgaaaga accgacagct gaagagatca
     1681 agaaaaaaag aatctatgcc gaatggggat tgactgatga agaatatcga atgatcgaag
     1741 aagatatctt aggaagaatg cctaattata cggaaactgg actgttttct gttatgtgga
     1801 gcgaacattg ttcctataaa aattctaaac cggttctacg caaatttcct actagtggac
     1861 ctcatgtgtt gcaaggccct ggagaaggcg caggcatcgt cgacatcggt gatggactag
     1921 cagttgtctt caaagcagaa agtcacaatc atccttcagc cgtggagcct tatgaaggag
     1981 ctgcgacagg agttggcgga atcatcagag atattttcag catgggggct cgtccaattg
     2041 cgttgctaga ttcattgagg ttcggcgaat tagataatcc aagaacgaaa tatttactag
     2101 aagaagtagt tgcaggtatc ggcggatacg gcaattgtat cggcataccg actgtaggcg
     2161 gcgaaattgc atttgatcct tgttatgaag gaaacccatt agtcaatgca atgtgcgtag
     2221 gtttgattga acacaaacat atccagaaag ggcaagccgc tggcgtcggt aattcgatca
     2281 tgtatgtagg tgcaaaaaca ggtcgcgacg gtatccacgg agcaactttt gcctcagaag
     2341 aatttaatca agaagaagaa cagcaacgtt ctgctgtaca agtaggcgat ccttttatgg
     2401 aaaagctgct gcttgaagcg tgtttggaat taatcttgca tcattcggat tctctaatag
     2461 gtatacagga tatgggagca gcaggacttg tttcatctag tgcggaaatg gcttcaaaag
     2521 ctggctcagg acttatattg acattagatg aagtcccaca aagagaaact gggatgacac
     2581 cttatgaaat gatgttatcg gaatcacaag aacggatgtt gatttgtgtt aaatcaggag
     2641 aggaagaaaa aatccaagaa ctatttcaga agtatgatct agatgcagtc actatcggta
     2701 aagtaacaga cgacgggcaa tatcggctat atcatcaagg agaagaagta gcaaatctgc
     2761 cagtcgatgc attagctgaa gatgcaccag tttatcataa agaaatgaaa gaaccagctc
     2821 gaatcgtaga atttcagcag ttagctcctt atcagccagt tatcgaagaa cctggacaag
     2881 tattgcttga tctgctgcag caaccgacga ttgcttcaaa acgttcaatc tacgaaactt
     2941 atgattcaca agtacaaacc aatacagttg tccgtccagg aagcgatgct gctgtgatga
     3001 gggttagagg gacgaataaa gcgttagcta tgaccacaga ctgcaacgcg cgctatctgt
     3061 atttggatcc aaaaatcgga ggacaaatag cagtggcaga agctgctaga aatattattg
     3121 ctagcggtgg taagcccttg gcgatcaccg actgcctgaa ctatggttct cctgataaac
     3181 cagaagtttt ttgggaatta tcgacttcag ctgacggaat tgctgctgcg tgtgaaacgt
     3241 taggaactcc tgttatttca ggaaatgtct ctttatataa tgaaacggat ggtcaagcaa
     3301 tctatccaac accaatgatt ggtatggtcg gattgattga ggatcataaa catatcacta
     3361 ctcaggaatt caaaaaatca ggggatctaa tctatatatt aggtaagacg tttgcagatt
     3421 ttgatggcag tgaattacaa aagatgcagc ttggaagaat cgaaggtgtg atccgaaact
     3481 ttgatctatc cattgaaaag agaaaccaag agttagtatt gacagccatc caaaacggat
     3541 tgattgaaag tgctcatgac tgttcagaag gtggcttggc aattgcactg gcagaatcag
     3601 ctttcaaaca tcagctaggg atatctgtac agtttgaatt gtcctctgct caattatttg
     3661 cagaaactca atcaagattt gtgttgacag tcgcaccgga aaataagaca cgattcgaag
     3721 aaatgatggg agatgctgct gttttagcag gtaaggtgac ggatgaagcg ataatcgaaa
     3781 tttcagcaac tgacggtcag atcaaaatag aaactgcagt agcaagaaag tgctgggagg
     3841 atgcaatcgt atgtcttatg aagtgaagag tttaaatgaa gaatgcggga tcttcggtgt
     3901 ttggggtcat ccagacgccg ctagtgtggc atacttcggg ctccatagtc ttcagcatcg
     3961 aggacaagaa ggtgcaggta tcgtgtccaa tgatcgagga aagcttaagg ggcatcgaga
     4021 tttggggctg ctttctgagg tattcaaaga tcaaagaaaa ttgcagcagt taacaggaga
     4081 agctgcaatc ggacatgtac gttatgcaac agcaggcaat ggaagtgtgg acaatattca
     4141 gccttttcta ttcaaatttc atgatcagtc gatcggctta gcgcataatg gtaacttgat
     4201 caatgctcgt tcccttagaa aagagttaga ggaaaatggg gcgatcttcc attctaactc
     4261 cgatacggag atattgatgc atttgatccg acgaagtgaa gaaacagatt ttttaaataa
     4321 attaaaaagt gctttgaatg aagtcaaagg tggctttgcc tatttattaa tgacagaaac
     4381 ggcaatgatt gccgctttgg acccaaatgg ttttcgtcca ttagcaatcg gacaaatgaa
     4441 aaacggagca tacgtgattg cctcggaaac ttgtgcatta gaagttattg gggcacgttt
     4501 tataagagat gtccagcctg gtgagatagt gatcatcaat gacgaaggaa tccagattga
     4561 ttgctttacg aaggacacgc agctttctat ttgttcgatg gaatatatct attttgccag
     4621 accagattca aacatcgcag ggatcaatgt gcatactgca cggaaaaata tgggacggaa
     4681 tttagcgaaa gaatcgccag taaaagcaga tatggtcgtt ggcgtaccta attcttcttt
     4741 gtcagccgca agcggttatg cggaagctag tggaatccct tatgaaatcg gattagtaaa
     4801 aaatcaatac attgcccgaa catttatcca gccaacgcct gagctaagag agcaaggtgt
     4861 tcgaatgaaa ttatctgctg tacgaggagt agtggaaggt aaaaaggtga ttatggtcga
     4921 tgattcaatt gttcggggta cgacaagcag acggatcgtc catcttttga aagaagcagg
     4981 ggcaaaagaa gtccatgtga ggattgcttc tcctcctttg aaatatccat gtttttacgg
     5041 gattgatatt caaacgcgta aagaactcat tgcggcaaat cacacgattg atgaaatcaa
     5101 agaatgtatc ggtgcagatt ctttaggttt tttaagtgaa gagggtctga tcgaatcgat
     5161 cggtctggaa aaagatgctc cgtattcagg attgtgtatg gcttatttca acggagatta
     5221 tccgacgccg ttatatgact atgaagaaaa atatcaggaa tcactaaaag aaaaagtatc
     5281 gttcttttaa aagcagacca agaagggagt atatgaattc aatggcaaat gcatacgcaa
     5341 aagctggcgt agatgtcgaa gctggatatg aagtagtaga acggatcaag aagcatgtca
     5401 aaaaaacgga acgtttaggt atgatgggcg ctataggagg attcggcggt tgttttgatt
     5461 tatcggtctt tcaattaaaa gagccagtcc ttgtctcggg gaccgatggc gtggggacga
     5521 aattagcagt ggcaatcgaa gcagacaaac acgatacaat cgggatcgac tgcgtagcga
     5581 tgtgtgtcaa tgatatctta gcacaaggcg cagagccgtt atatttctta gactacttag
     5641 caacggggaa aaatgatccg ttacgtttgg aaaatgtagt aaaaggtgta gcagatggat
     5701 gtgttcaagg aaatatggcg ctgattggcg gagaaacagc tgaaatgcct ggtatgtact
     5761 caggtaatga ttatgatcta gccggatttg ctgtagggat agctgaaaaa tctcaattat
     5821 tgacaggaga aacgatcaac ccaggagatg tgttgcttgg aatagcatct agcgggattc
     5881 attcaaatgg gtactctctt gttcgcaaaa ttttctttga gcagcagcag ttttctttat
     5941 catcaaaact gccagaattg aaaaaaacac ttggcgaaga attgcttgag ccaacgaaaa
     6001 tctatgtttc ttccttgctt ccgctgatca aagaaaagaa aataaaagga gctgcacata
     6061 tcactggtgg agggtttgta gaaaatatcc cgcgaatgtt accagaatct atggctgcac
     6121 aaatcaaact tggctcatgg ccgattcttc caatattcgg ctgtttacaa aaatatggtg
     6181 aaattcctga gatggagatg tatgaaatct tcaatatggg catcggtatg gtcctggcag
     6241 tcgaagaatc gaaagtaaaa gaaatagtac acgaaattga gcaagctggc gaaaaagttt
     6301 atgaaatcgg gaaagtgatc gccaaagaaa aacatcgtgt ggtgtttata ggagaagaac
     6361 gatgagaatc gctgtttttg cttctggaaa cggaagcaac tttcaagcgc tagccgatta
     6421 tctatcgaaa aaaggattgg aatcttcgat tgactggttg ttttgtgatc agcctgaagc
     6481 atatgttttg aaacgtgcaa ctgcgttgag tgtaccggca gattgttttt cacctaaaga
     6541 atttgattca aaaaaagagt atgaagaagc aattttgcat aaattgaaag aaaaaaagat
     6601 cgatttgatc gtattagctg gatatatgcg aatcattgga ccggtattgc tgaaaaatta
     6661 tgataaaaga atcatcaata tccatccgtc tcttttacct gccttccctg gtttgcatgg
     6721 catacgtgat gcctttgaag ctggagtaaa agaaacgggt gtcacaattc attatatcga
     6781 ccaaggtgta gatactggac caatcatccg gcaggaaaaa gtgagaattg aacaggagga
     6841 cacgttcgat tctttagaag aaaaaatcca tagagtagag catcgaatct atccagaagt
     6901 gattagtgaa attattgaaa atggaggcta tctgaaatga caagagcatt gatcagtgta
     6961 tcagataaaa ctgggattgt agcattcgcc caaggtttga ttgaacaagg agtagaaatc
     7021 atttcaacag gaggaacaaa gcatgcgttg gataaagctg ggatcaaaac gctttcgatc
     7081 gaagaagtga cagggtttcc tgaaatgatg gatggccgtg tcaaaacact tcatcctaaa
     7141 atccatggtg gtcttttagg aagacgcgat ctccctagcc atatggaggc aatggaaacg
     7201 gaaaatatcc aaccaatcga ctttgtttgt gtcaatttat atcctttcaa agaaacgatt
     7261 atgaaaccag atgtcgaaat agcagaagct attgaaaaca ttgatatcgg tggtcctagt
     7321 atgctccgta gtgcagcaaa aaaccatgaa tttgtcacct ctgttgttga tccatctgac
     7381 tatgaagaag tactttcaga attacgtcag tatgggcata cttcactttc tttcagacaa
     7441 aaactagcgg caaaagtctt tcgtcatact gcggcatatg atgcactgat cgctcaatac
     7501 ttaacggatt gggtggaaga aaaagaaccg gaaaaattga cacttacgta tgaacgaaaa
     7561 caaacgcttc gttacggtga aaatagccat caagccgctg ccttttatca gtccgctctt
     7621 ctggaaaaat attcgatcgc agcagcaaaa caactccatg ggaaagaact ttcttataat
     7681 aatatccgag atgcagatgc tgcgctacgg atcatgaagg aatatgacga gccaacggta
     7741 gtcgccttga aacacatgaa tccttgtggt attgggacag catctacgat tttagaagcg
     7801 tgggaagcag cgtatgaagc agatcctgtt tcaatctttg gtgggatcat cgttttgaat
     7861 cgagaagtag atctgccaac agcagaacag atgcataagc tgtttctaga aatcatcatt
     7921 gccccttctt atacacaaga agctttggaa ttactgcaaa aaaagaaaaa catccgatta
     7981 ctgacagtag actttgcgca ggaagaaaat caaaatcgag aagaaacggt ctcagtctta
     8041 ggtggacttc ttgtacaaga tcaagatctg gcaatagaaa atgaagaaga gtggaaagtc
     8101 gtgacaaaac gtaagccgac tccagcagaa gcaaaagcat tagtgtttgc ttggagagct
     8161 gtcaaacatg tgaaatctaa tgcgatcgta ctggcaaacg aacgtcagac agtaggaatc
     8221 ggagccgggc agatgaatcg agtcggttca gtaaaaattg cgattgaaca agcgcaagag
     8281 aaaatggaag gtgcagtttt agctagcgat gcttattttc caatggatga ttcggttgaa
     8341 tatgcagcca aacatggtat aaaagcaatc attcagccag gaggaagcat caaagaccaa
     8401 gattctattg atatggcgaa taaatacgat ctagcaatgg tctttactga tgttcggcat
     8461 tttagacatt agagaggagt cggataaatg aacattttgg tgatcggtgc aggtggtcgt
     8521 gaacatgcaa taagtaaaaa acttttagcc agtccgaagg tggatacggt gtattgtgcg
     8581 ccaggaaatc cggggatgac aaaagatggt atcaattgtg tcgctatcag tgagcgagat
     8641 cattcagcgt taatccattt tgttaagaaa gaggctattg attggagtat cgttggtcct
     8701 gaagttcctt tgttaaatgg gatcgtcgat gattttatgg cggaaggatt gaaaatcttc
     8761 ggtccgacaa aagcagcagc tctgatcgaa ggttcgaaaa gttttgctaa acaattaatg
     8821 gatacttatc aaatcccgac agctaaatcc agaagttttg aatgttacga aaaagcagca
     8881 gcgtatatca aagaaaatgg tgcgccaatc gtagtgaaag cggacggctt agcagctggg
     8941 aaaggtgtag tcgtggctac cacagtgaag gaagcgctgg atgcggcaga acaaatgttg
     9001 aaccagcatc gatttgggga tagtagcaaa aaaatcgtcg tagaagagtt tttgacagga
     9061 gaagaattct cccttttggc gtttgtccgt aataccaagg tgtatccgat ggtcatttcg
     9121 caagatcata agcgggcatt tgataacgat aaagggccaa atacaggcgg aatgggtgct
     9181 tattcaccag taccgcaaat ttcaaaaaag atgattgaaa aagctgtgga agaagtttta
     9241 aaaccagcgg ctttgggaat gaaagaacta ggacgctctt tcacgggtgt tttatatgcg
     9301 ggtttgattg ctacgaagga aggaccaaaa gtgatcgaat tcaatgcgcg tttcggtgat
     9361 cctgaaacgc aagtcgttct ttctcggttg aagagtgact ttgcgcaagt aatcgatgat
     9421 ttattagaga atcgtacagt agagttaaag tggcagcaag aaggatacaa tgtaggcgtt
     9481 gtggttgcag ctgccggtta ccccgaagaa tatgagacag gaatggttct accagatttt
     9541 ctggaagaag agctttctgt ttactatgct ggagtttcct caaataacgg caaactagtt
     9601 ggagcaggag gacgtcttta tttagtagaa gcatacggtg agacgatcaa agaagcacag
     9661 aaaaaaattt acaccgcgtt aatggataaa acgacagatg gaacatttta tcgtacagat
     9721 attggagaaa aagctgccaa tagcgaactg atcgtgaagt aaatcagctg cttccattta
     9781 taaaaaatac aacaaacata atcggaagcg ccgattttag tgaagccttt tcataaagag
     9841 tgtggtacac ttttgttgtg tatataaata aggaggagtt cagatgaaaa aaattcaatt
     9901 aggtacttct gatcagaaag cagctgcagt gatcttaggc tgtatgcgta tcaacaatgc
     9961 ggaagatcca gtaaaagtga ttgagacagc ttatgaaaac ggcattgatt tttttgacca
    10021 tgcagatatt tatggaaacg gtgaatgtga agaaatcttt gcagatgctc tagcaaaaac
    10081 atcaatcaaa cgagaagatc tttttattca aacaaaatgt ggaatcgttc caggtgtgat
    10141 gtttgatttt tctaaaaaac atatcattga atctgtagaa aaaagtttga cccgtctgaa
    10201 aatggattat gtggatgctt tacttcttca tcgtccagat acattggtag aaccagagga
    10261 agtagctgaa gcgtttgatg aattggaacg gtcaggaaaa gttcgttatt ttggcgtcag
    10321 caatcaaaaa ccaatgcaaa tcgaattgct gaaaaaatat gttcgtcaac cattagtagc
    10381 taatcagctg caatttggct tgaaacatac tggaatgatc gatcaaggga ttcatgtcaa
    10441 tatgacagat gaagcaagta tcgatcatga cggtagtgta cttgattatt cccgattgca
    10501 tgatatgacg atccaagcat ggtcaccata ccagtatgga ttttttgaag gtgtatttat
    10561 cgggaacgaa aaattccctg aattaaacca aacattagaa gaactagcag ctaaatatca
    10621 aacgacacca actggcttgg cttctgcttg gattttacgc catccagcca atatgcaagt
    10681 gatcgccggt acgatgaacg tagggcgaat caaggaaatc gctcaagctt cagaaattaa
    10741 tatgtctcgg gaagattggt acgaattgta ccgtgctgct ggaaacattt taccttaaag
    10801 attgtcataa gcaggtcaaa agggctataa attaaaaaga cagaataact tgaaagctgt
    10861 tttcagcttc gagatattct gtcttttttt attgcaaatg gaaagagtat cattgtacgg
    10921 atttcgttta ttagttattt ttgtttttaa ggaaaagctg tgtccgatat tcttttggag
    10981 aaatcccttg ttttttctta aataaataac tgaaatagtt agggtcatta taaccaattt
    11041 caaaagcaat atctgaaata cgatagtctg attgctgcaa taattttttt gctaattcga
    11101 ttctttgttc tgtcagataa tcgatgaatg tttggttaaa cgattgagaa aaaatcgtac
    11161 tgaaatgaga gggacttaat gcaacttgtt gagcgaccca agttaaggac atatctggat
    11221 cggtaaaatt ttctttgata aaatccaatg catggttcaa aattggctga tatttgttcc
    11281 ttgaaggctg tgagggcaga gcagttaaga aagaaatgaa catataaact gtcgaacggt
    11341 aaagcgagtc gtcggcgctg attgctaagc gttgactggt ggatgtgcaa tgaaccattt
    11401 cttgcgggat tttcttttct ttcaggcaga catgctggag tagcttagtc aagacaaata
    11461 aacgatataa tcgtgttcga gttggtgtgc cttgtggttt cgtcaagtgt tcgatgtact
    11521 tagatacttc gtttttcgat aatgaagaca aatcaagtga gaggtcaaag taaaagacag
    11581 aatcaactgg aagtttcgct tcttgaatca aatcctcata gctgataatt tgttctttaa
    11641 gggaactggc ggtttcaagt atcatctgcg tgatctcaaa agatgcaggt aattcgctga
    11701 ttctttctgc gacaggacca atggcgacag aaaaattatt tttttgtccc ttttctaatt
    11761 catggaccaa cgtttgggct acctgataac tggtctcgat cacattttct tcaagagaat
    11821 caactattaa aaatttgatg aatcgagagg aaacagcaga gaagatcact tgttgattct
    11881 ccctaaataa aaacttcaaa taatcactga gatgagcgta atcttcaaag tctgtactga
    11941 cttggtttgc tgctaataat acggtcattt ttttccctgc taaagagcga ttgaaagatt
    12001 cagcaagttt caatgtttca ctgatcgtaa gtgagccttt gaacaaaccg ttcaagaaat
    12061 gatttttttt caattcgaaa acaagatcag aagaagtgtg cgtttctttt tgctgcaaat
    12121 ctaaagcagt tactgctttt tctattgctt gtttcaattc gctactttta atcggtttaa
    12181 gcagataatc atctgcctgt acctggattg ctcctttcat ataatcgaaa tcgtcgaatc
    12241 cactaataaa tatgagacga gtccaaggaa gtagtttcct tgcttctttt gaaaaagtca
    12301 acccgtccat gaaaggcatt tggatatcag tcagaatgat gtcgggctgt aagtcgataa
    12361 ttgaagacag tgccatctca ccatctccag cttcgccgac ataagtgatt ggataataag
    12421 aaccaaggac agttatttgt tcccgcaagt tattgcgaat gaggtattcg tcttcgatga
    12481 taaaaacttt atacatgttt gtttcctcca attgttttgg gagatagagg gatcatcagt
    12541 gtgacagttg ttccttctcg gtatttgctg tcaatcgtga taggtttttg atcatttccg
    12601 taataaagaa ggagacgctt gtttacgttg taaagtccgt agccgatact gaaatctgaa
    12661 tcgatccctt tggctaactc tttacgtatt tctgccagtt tttctgcagg gatgccaatc
    12721 ccgttgtcta taactttgaa ttggatcatc tcatcaattt tgcatgtggt aatacgaata
    12781 aatcctttgt gacggacgta cttcgttcca tggtatacag cattttcaat caatggctgc
    12841 aagatcattt ttaaaataga aacaggaaaa agtgtttctg gtatatcgat cttatagtca
    12901 agcatatggc cgtatcttat ttttaagatg actagataat cattgatatg gctaatttct
    12961 ttttcaagag gaatccagtc ttgtcctttg cttaaagacg tacgaaaaaa gccggaaagt
    13021 gcatgggtca tagtcagagc ttcttcaaag cagtcttgtt cgatcaaaga aataatggca
    13081 tccaaactgt tataaataaa atggggagta atctgagctt gcaatacacg aacttcactt
    13141 tgtgctaaat ggtattgttt caaagcgttc tcccgtaaag actggtttag atcttccgcc
    13201 attttattca tgctagcggt aagtgtttgt aattccagtg tttccggcag ggttaaacga
    13261 aagccaaggt tccctgaaga aagttgttct gacatctcga tcagctgatt gatcggttct
    13321 tggacctttc tctctaaaaa gcgacgattc tttttcgcat agaaaaaaat ggtactaaga
    13381 actccaagtt ccactattgt taatagaatg agcgagcgga caagtgtcgt attttttttg
    13441 cttgctaagt cgatctccac acgaacaaaa tcttgtaaga tatctgacag cagttgagtg
    13501 acagaatcta cctgattgat gatctcttca ttggtatcca taaaatcttt tgccaagata
    13561 ttattgatca gctcgttttg ataatcttcc agtgtatcga tcgtacgtag cgatacatct
    13621 aacgtactaa tttctttaag tgtatttgta ttatttttgc tctctcgaat atcattccta
    13681 agttcttcta ggatattttc ctcattgaac tcatctacgg ttacttggcc agaaatcaag
    13741 tcccacatac tttccaaaac tttttgatca accttcaaag caattgcatc tgctttttgg
    13801 atatttgcga tcgtctgttg ataaatcaaa agctgttgcg aatataacag tatgctcagt
    13861 agcaaaggga taacagcaaa tagaagcatg gctttgtttg cttgggcaaa aatctgtttt
    13921 atgcttaaat tttttgtcat gatacttctc cagtcaataa tttctggttg ggatgttagc
    13981 tatattctgc tgggaaaaga cagtctcgga aataaaaata tctttaggta atgtttttcc
    14041 tgtttgaata tttccaaaat aacgagtaac ggtattggct acataccagc cgacatcagg
    14101 tttacactcg actacacaat tgacgattcc tgagcgtagc ttctcgatca tatcttcttg
    14161 tccatcaata gttacaatga tcaaatcttc tccaggtttg attgatgttt cttttatcgc
    14221 agctaaagca ccgtgagtca tttcatcact atggctgtat aatacatcga tttcggataa
    14281 atcatttgtt tctaaatatc gtttcatctg gtcttttcct ttcatccgaa taaaatcacc
    14341 gacaattcgg tgaacaatag tcatcggagg tttatggtcg atggttccat ctttttttag
    14401 ggttggttta cgattgattg attccaaaaa tccttcggag cgtagcttgg tcggtgtgga
    14461 gttctctgaa ccagcaagtt ctaggattct gatttcctgt ttctttgaat ttgtgaagtg
    14521 attgctgaca aataaacccg cccgatttcc ttctgctttg aaactggggc caatgtgggt
    14581 gacgaaaagc tcgggatcat tcgtttgaat atgtctgtcg acaagtatga cagggatccc
    14641 tgctcgcttt gcttctttta atacagagtc ccacccggat tcttgcaaag gagtaaaaat
    14701 gatcatatcc actttatagg caatgaacgt ccgtatatct tggatttgcc gttcctgatt
    14761 catgtagcca tttcggtaga gaacttggta atcttctttt tcaagcgcag tgataattga
    14821 ttcagtatgc tttttccgcc agctactctc cgttcctgat tgcgaaaaac caacgactag
    14881 tgggtcaggt gcttcctttg tattaggtgt acaccccatc atttggtaag cgattacaac
    14941 aagggctaaa aaaaataaaa cacgtttcac tttaaatcac tccatctgga tttgttagta
    15001 ctatcttatc agataaaagg gaaaatcaaa gtcaaaagga atcaatcgaa aaaaatccaa
    15061 gaaacttaaa aaaatccaag acaaaaaaag tacacctaaa aaatttaaag taaatcccat
    15121 aaaaaaatca atgttaacgc tttctttatt agctatactc ggtgtatcaa ttgaaatggg
    15181 ggataaaaaa tgaaaaacaa atttagaaag attgttggga ctatcgctat tttgaccgca
    15241 agcattctat taagtgcgtg tggcaattca ggatctgcag atgatagtac ggggtatgta
    15301 ggaatcgcta tgccgaccaa atcagcagaa agatggatcg ctgatgggaa caacatggtc
    15361 tcggaattag aaaaactggg ttataaaaca gatcttcaat atggagaaga taaagtagaa
    15421 aatcaagtag cacaaataga aaatatgatt acaaaagggg tagatacact tgtgattgct
    15481 tctatcgatg gttcggcttt aactgatgtg ttagctaaag caaaagaagc agatatcaag
    15541 gtgatcgcct atgatcgttt actgatgaat tcagaaaatg tcgattatta cgcgactttt
    15601 gataattttg gagtaggtgt atcgcaagca gcttatattg aagagcactt aggattgaaa
    15661 gaaggaaaag gaccattcac gattgaatta tttggtggct cgcctgacga caacaacgca
    15721 ctaatcaatt acaacggagt gatgtctgtt ttacaaccat atatggacaa cggtcagttg
    15781 gtcgtgccat ctggacagac gagtttcagt caaatcgcta cgcttcgctg ggatggttct
    15841 acggcgcaag caaggatgga taatctgtta agcgcaaatt atactgacca aacattggat
    15901 gctgttttgt cgccatatga tccaataagt ttaggaatca tttcgtcatt aaaaggcgtg
    15961 gggtatggga gtgaaagtaa acctttacct gtgattactg gacaagatgc gacagttgct
    16021 ggcgttaaat cgatcattgc cggggaacaa acacaaacga tctttaaaga cacacgtatc
    16081 ttagcaaaaa atacgattga aatgatcaaa gcaatcagtg atggagaaga ggttccggtc
    16141 aatgatactg aaacttatga taatggggtg aaaactgtcc ctacgtattt agcaaataca
    16201 gtatctgtcg ataaagataa ttatcaagca gagctgatcg acaccgatta ttataaagaa
    16261 tccgatctga aaaattaaga ggaaccaaac aaggaactaa agagatctaa gtcagagaga
    16321 ggataagaat tatggcggat tgtattttaa aaatggcaga tatcaccaaa aaattttctg
    16381 gaatccgagc gttagattgc gtgaatctga tgattgaacg tggggaaatc catgcattgt
    16441 gcggagaaaa cggagcggga aaatcaacat tgatgaatgt cttgagcggc ttgtatccat
    16501 atggctccta tgacggggaa atcttttatg atggaaagct gtgtcaattt aaagatttga
    16561 aagatagtga agaaaaaggc atcgtcatca ttcatcaaga attagcacta agtccttatc
    16621 tttcaataaa agagaatatt tttctcggaa atgaacaagc agaaagaggc gtgattgatt
    16681 gggaactgac agaaaagaaa acatatgaac tgctgaataa agtcggtttg tctatcgacc
    16741 cgaatcttct agtatcccag attggcgtag ggcaacagca actagttgaa atagctaaag
    16801 cattatcaaa atccgtccgt ctgttgattt tggatgagcc taccgctgca ttgaatgaag
    16861 aagaaagcgc gaatttgctg agattgatcc gagagttgaa agagcaaggt gtaacctcca
    16921 tcattatttc acataagctg aatgaaatcg tggatatcgc agatcgaatc accattttac
    16981 gggatggaca gacgattgag acactagaaa atgaaatgat cagcgaagag catattattc
    17041 gcggaatggt gggcagagat ttagttgatc gttatcctga aagaaaaccg gatattggag
    17101 aagtttattt taaagtagaa gactggacgg tctatcaccc tcttgatcac gagcgaatca
    17161 taaacgacca catcaacttg catttgaaaa aaggagagat tgtaggaata gctggactga
    17221 tgggagcggg cagaactgaa ttcgctatga gtctatttgg acattcttat ggaagcaaga
    17281 tatccggacg tatattaaag gaaggggcag agatatcagt taagaccgtt ccttcagcca
    17341 ttgcaaacgg actcgcttat gtctcggagg accgaaaatc tttaggactg aatctgctga
    17401 tggatattcg ggaaaacgtg acgctgagca gtttggataa aataagtcaa aaaggagtat
    17461 tagataaaga aaaagaagta actgaggcag aaaatttccg gaaaaagatg cgtataaaaa
    17521 caaactccat ccatcaaatt gtcagtagtc ttagtggtgg gaatcaacaa aaagtcgttt
    17581 tatccaagtg gctgatgaca caacccgata ttcttttttt ggatgagcca acccgtggga
    17641 tagatgttgg ggcaaaatat gagatctaca cgattatcga agaaatggca gcaaatggca
    17701 aagctgtctg tcttatctct tctgaacttc ctgaaatatt agggatgtgt gatcgaatct
    17761 atacgatgaa cgaaggacgt attacaggag aagtctcgcg agaagatgca gatcaggaag
    17821 tcttgatgaa attaatgacg aaagaagagg tgggttaagt tggaacaggc aaaaaatctt
    17881 acggataaaa aagagaaaaa actaaatttg aaaagtagag cagctgatct ttttggtcgc
    17941 tacagcatgg tgatcatttt agcgggacta cttattgcgt ttcaattgat gacggatggt
    18001 attttctggc gtccattgaa tataactaac cttgttctgc aaaatagcca tatattagta
    18061 ctagctgcag ggatgttatt agttgtgctc ttaggtcatg tggatttgtc ggtagggtca
    18121 gtgatggcgt ttgttggtgc aattgcgggc atgatgatgg tcaatcgcca aatgaacccg
    18181 tggatgacag ttatcgcatg tcttgcagta ggcgggctga tcggcgcatg gcaaggtttt
    18241 tgggtagctt atgcagggat accggcgttt atcgtgactt tagcaggatt gctgatgttt
    18301 cgtggactga cacaagttgt tctaggaggc caatctctag caccttttcc agctgttttc
    18361 caaaaaatgt ctacgggtta tttaccagac ttaagcgaag gaagcagcct tcacttcttg
    18421 acattgattt taggaatgat catcacgatc gtgttagttg ttggtcaatg gcgaacaaga
    18481 aatcgtcgga aaaatcattt atttgaagtg gaatctccaa cagctttctt ggtaaaggcg
    18541 gtatttacag cagtcgttgt actaggaata acttatatct ttgcctctta tcaagggtat
    18601 ccgattattt tgatcattct tggtttgatc gttgcagcat atgggttttt gacaaataaa
    18661 acagtagcag gtcgacagat ttatgcgacg ggcggaaatc gaaaagcggc tgaactatca
    18721 gggatcaaaa caaagcgaat cacgttctgg gtattcgtca acatgggagt catggcagct
    18781 ttagctggat tagttttagc tgctcgattg aatgcggcaa cacctcaagc aggtacatcg
    18841 atggaactgg atgcaatggc ggcagtatat tttggtggtg cttcgacttc cggcggcatt
    18901 ggaacaatca tgggtgcgat tgtcggtggc ttggtcatgg gtgtattgaa caatggcatg
    18961 tcgattttag gcgtcggggt agactggcag caggcaatca aaggattgat cttgctttta
    19021 gccgttgtgc tagacttgta taataagaaa aaacgaacaa cgtaggagaa cgccggttat
    19081 tcagaaaaaa gactgtgaca agacatttgt cacagtcttt ttttcgctaa aagcgaacat
    19141 tcattgtttc gtttgtatag actaaaaata atatatgttt ttttaccatt cgtataaatt
    19201 ctctttttgc tcttgatttt tttgcttaat tgagttaaat tacatagggc gctaaaaaaa
    19261 gccttgatta aattaataat gttcgtgttt tgctggatgt gaacatcagg aggataatgt
    19321 aatgaaagta tttgattacg aagatattca attaattcct aataaatgta tcgtaaatag
    19381 ccgttctgaa tgtgatacaa cggtcacatt aggtaaacat acattcaaaa tgccagtagt
    19441 gcctgccaac atgcagacga ttattgatga aacaatcgca gaattcttag cggaaaatgg
    19501 ttatttctat atcatgcatc ggtttgatga agcagctcga attccgttta tcaaaaaaat
    19561 gaaaaagcgt ggattgatta cctcgatcag tgttggcgtc aagaaagagg aatattcctt
    19621 tatagagaag ttggcagaag aatcgctaaa tcctgattat atcactatcg acatcgcgca
    19681 tggacatgct aactcagtta ttgatatgat tcagcatatt aaaaaatatt tgcctgaaac
    19741 ttttgtgatt gccggtaatg ttggtacacc ggaagccgtc cgtgaattgg aaaatgcggg
    19801 cgcagatgca acaaaagtcg ggattggtcc aggaaaagta tgtattacaa aaatcaagac
    19861 aggctttggg actggaggct ggcaactggc agctcttaga tggtgtgcga aagcagctcg
    19921 caagccgatc atcgctgacg gtgggatcag aacacatggc gatattgcaa aatctgtccg
    19981 ttttggtgcg acaatggtca tgattggttc attgtttgcc ggtcacgagg aatctcctgg
    20041 cgaaacaaaa gtcgaaaatg gaatcgtgta taaagaatac tttggttctg cttcagaatt
    20101 ccaaaaaggc gaaaaacgaa atgttgaagg aaagaaaatc tggatccaac acaaaggatc
    20161 tttgaaagat acactagtgg aaatgcaaca agaccttcaa tcatctattt cttatgctgg
    20221 aggaagagat ctggaagcca ttcgaaaagt tgactatgtc atcgtgaaaa attcgatttt
    20281 taatggagac acaatctaaa aaataaataa aatattagga tgttgtgaaa aagttgtcct
    20341 gcattaagta ataagaacag ccaaacaaaa atagttcttt atatgttttg tttggctgga
    20401 attattatcg cagatacaag cttttgaaca tcattattca gaataaggga ctgtgataag
    20461 atttttgtca cagtccctta tgttttgcaa aaaaagtttt tcttttttct cgtaaattcg
    20521 gtaaaatcaa aagaaaaaat agacaataga aaagagaagc ttatgtggga aaaagtagag
    20581 aaaaaacaag gaccgattta tcgtcaaatt atggaacaga ttatgagaga aatcgaaaac
    20641 ggaaggatca atcctggaag tcaacttgaa tcagaacgaa aactagggca actttttggt
    20701 gtgaatcgtt ccaccgttgt ccatgcattg gaagagctta gggagcttgg ggtactaacg
    20761 agcaaacgtg gcagcggatg gtttgttaat cagactgaat gggggaaatt cgctgttcca
    20821 agaatcgatt ggcggcaaat gatctctcct cgttatgagc agacggactg gtatgagcaa
    20881 aaaataagag aagcaaagca gatccaaaaa acatcgtttc ttgatttata cgctagcgag
    20941 atgccagatg agcttttgcc gaattttgag tttccttctt attctcttga agaaatctta
    21001 gcggaagaaa aagaaatgga tcttttaggc tatactccgt tacgtaacaa aatcaaacgg
    21061 tatttggaaa gtaaatttga attggcattt tcccccaacc agcttcttgt gactggtggc
    21121 ggtcagcaag caatcttttt gattttacaa acgattcttt cagctggaga ggggatagct
    21181 gtagagtcac cttctttttt ttaccgatta cctcttttca aagcttcagg aacgcgttta
    21241 tttgggatac cgatggatga agaagggatc gacttagaaa aactgaaaga atcgatccat
    21301 aaaaataaat tgaaagcagt gcttatcaac ccgaattttc aaaaccctac aggaaaagtg
    21361 atgagtcaaa aacgacgaga aaagctagta gaattgtgtc gacagtatcg gcttccaatt
    21421 attgaagacg atgtatttac ggatctttca tttctaaacc aagaagaagt ccttcctata
    21481 cgaacgattg atccggataa tgtgttatac gtcggttcgt tatctagggt tttaggaaaa
    21541 acaacgaaaa tcggttgggt gataggacct gagactttta tctcgcaact agctcaagcg
    21601 caagaaatga tggagttttc gatgaatatc cttacacaaa tcacagtggc taatgtattt
    21661 aatgaagcaa ttgatttgaa aatgcaagat gtaagaaaaa agttgaaaga aaaaagccga
    21721 ctgataagcg cttggggaga ttcacaatcg tttttcaagc ttcattcaat tttaggtggt
    21781 tactatgcct ggattacttg ggatgggaaa aaactagacc ccgaactagc agaaaaagta
    21841 gtagatatgg gtcttggaat tgcaccaagt ctactttttg ggcaagatat aaatggaatc
    21901 agaatcaatt acagtaggct ggatgaaaac caaataccgt tgttttattc aaagatgaaa
    21961 attctagaaa agtggttaac agaataattt cacaaagtaa atagattaaa gaatataaca
    22021 gcttggattt aaatttcaag ctgtttcttt tttttcggaa aaagaatacg atataaagaa
    22081 actaagaaaa aaccattatt tgtacgtccg ttcacgatgt gtaaataaaa taaatgtttg
    22141 caacaaaact gtataacacc aacgttttta aatagaaaca gatggttgta agtttgatta
    22201 gaaaatagaa aaaatgcaga tgaaatataa aacgctttcc ttattgtgat tttattcact
    22261 aatgctgtta atatgcggat tggcataatt tcctttcatc gtgaaaattt gtgcttttag
    22321 cttgtatttc tcataaatcc atgataattt attaatgaag acggttaaat agtgatacag
    22381 ttttattcac tgtttcaatc gcagaatgaa gagattttct tatagatgag agaggaatgt
    22441 gtgacaaatg gcaaacaaaa aagcaccgat cgacttccaa gcactgctag atgaagtcaa
    22501 tgcagatttc ccagtatatc aagcacttga tcaagatgga aacgtagtta acaaagattt
    22561 agtacctgat ttatccgatg atgaactcgt tgaattaatg actcatatgg tatggtctcg
    22621 tgtattagac caacgttcaa cagcgctgaa ccgccaagga cgcctaggct tttttgcacc
    22681 aactgctgga caagaggcaa gtcagctggc tagtgcttac gcatttgata aagaagatgt
    22741 tcttcttcca ggttatcggg atgtacctca actagtaaaa catggtttac ctttatctca
    22801 agctttttta tggtctcgag gacatgctgc aggtaattct tatccggaag aattgaaagc
    22861 tctaccacca caaattatca tcggtgcaca atatgtacaa gcagccggtg ttgcattagg
    22921 tttgaagaaa cgaaacaaga aaaatgtcgt cttcacttat actggtgatg gcggttcatc
    22981 acaaggtgat ttttacgaag caatcaattt cgctggcgct taccatgcaa atgccgtatt
    23041 ttatatccaa aacaacgggt atgcgatctc tacacctcgt gaaaaacaaa ctgcagcaaa
    23101 aacacttgca caaaaagctg tcgcagctgg gattcctggt attcaagtcg atggaatgga
    23161 tccgcttgca gtatatactg tatctaaaat ggcacgggaa tgggcgatca gtggaaatgg
    23221 accagtccta atcgaaacac tgacttatcg ttatggacca catacactat ctggtgacga
    23281 tccaacccgc taccgtacaa aagacacaga tgacgattgg cagaaaaaag atccattgat
    23341 ccgcttccgt aaatatttga cggaaaaagg actttggtct gaagaaaaag aagaacaagt
    23401 catcgaagca actaaagaag aaatcaaagc agctattgct gaagccgaca aagtaccaaa
    23461 acaaaaggtt tcagatttct tgaaaaacat gttcgaagta tctccacaat caatcaaaga
    23521 acaaattgca atttatgaag cgaaggagtc gaaataatca tggcacaaaa aacaatgatc
    23581 caagcaatca cagatgcctt agcacttgaa ctagaaaatg acgagaatgt cgttgttttt
    23641 ggtgaagatg ttgggaaaaa cggaggagtt ttccgggcaa ctgaaggatt gcaggaaaag
    23701 ttcggagaag accgagtatt cgatactcct ttagctgaat ctggaatcgc aggattgagt
    23761 ttcggtcttg cgttagaagg atttcgtcca gttcctgaaa tccaattctt tggttttatt
    23821 ttcgaagcaa tggacgaagt agtagctcaa atggctcgta caagatatcg aatgagcggc
    23881 actagaaatc tacccatcac aattcgttcg ccatttggtg gcggtgttca tacaccagaa
    23941 ttgcattcgg ataatttaga aggtttgatc gctcagtctc ctggtattcg agtagtgatt
    24001 ccttccaacc cttatgatgc aaaaggacta ttgatttctt ctattagaag taacgatccg
    24061 gttgttttcc tagaacacat gaaactatat cgctcattcc gagaagaagt gccggatgaa
    24121 gcttacgaag ttcctttgga taaagcagca gttactcgtg agggaacaga tgtatctatt
    24181 atcacatacg gagcaatggt gcgtgaagca atcaaagcag cagacaatct tgcaaaagaa
    24241 aatatatctg ttgaaatcat tgatcttcgt acagttgcgc cattagatgt agaaacgatc
    24301 attcaatccg tggaaaaaac tggtcgcgta gttgttgtcc aagaagcaca acgacaagcg
    24361 ggcgttgccg cgcaagtagt ttctgagatc tcagaacgcg ctatcctttc attagaagcc
    24421 ccaatcggac gagtttctgc acctgataca gtcttcccat ttggacaagc agaaaatgtc
    24481 tggttgccaa atgcaaaaga tatcgaagac aaagtaaaag aaatcgcgga attttaagaa
    24541 aagaagggac gaaacaaagt catggcttat caatttaaat tgccggatat cggtgaagga
    24601 atcgcagaag gtgaaatcgt caaatggttc gtcaaaccag gagatacgat caatgaagat
    24661 gatacattac tagaagttca aaatgacaaa tcagtagaag aaatcccgtc acctgttaca
    24721 ggaacggtta aaaatgtgat tgttccagaa ggtaccgtag caaatgttgg ggatgtatta
    24781 gtagaaatcg atgcaccagg acacgaagac aatgaagggg acagtggtgt agccgcagaa
    24841 tcccaaactc cagctaaacc ggctgctgaa cccactgttg atacagaatc agctggatcg
    24901 tcaagcgaag gagtcttcca atttaagtta ccggatatcg gtgaaggaat cgcagaaggt
    24961 gaaatcgtca aatggttcgt caaaccagga gatacgatca atgaagatga tacattacta
    25021 gaagttcaaa atgacaaatc agtagaagaa attccgtcac ctgtcacagg gacagttaaa
    25081 aacgtgattg ttccagaagg cactgtagca aatgttgggg atgtattagt agaaatcgat
    25141 gctcctggac acaatagtgc accgtcaact agtgcgccat cagctgaagc accaaaagaa
    25201 aaagtggaaa catcaggttc agcaagtgtc gttgaagctg ccgatccaaa taaacgtgta
    25261 ttggctatgc catctgttcg tcaatttgcc cgtgaaaaag atgtagatat ctcacaagta
    25321 actgcaactg gaaaaggtgg cagagtaaca aaagaggaca tcgaaaactt cttggcaggt
    25381 ggtccaagca gtgcaccagc gaaatcagaa gcgccagaag cggcagcacc gaaagaagca
    25441 gctcctgcag cagaaagcaa accagctgca ccagcaaaac cattcaagtc caacttagga
    25501 gacttggaag aacgtgtagc aatgacgcca acacgtaaag ctatcgctaa agcaatggtc
    25561 aacagtaagc atacagctcc acacgtgaca ttgcatgatg aagtagaagt gtctaaacta
    25621 tgggataatc ggaaacgatt taaagaagtt gctgctgcaa atggtacgaa acttactttc
    25681 ttgccatatg tcgtgaaagc tttaacagcg actgttaaga aatacccagt attgaatgca
    25741 tctatcgacg atgcaaacca agaaatcgtt tataaacatt actacaatat cggtattgca
    25801 actgatacgg atcacggact atatgtacca aatgtgaaag atgcagaccg caaaggaatg
    25861 tttgctattg cagacgaaat caatgagaaa gcaaaattag ctcatgacgg caaactatct
    25921 gcagaagata tgcgcaatgg aacaattacg atcagcaata tcggatctgt aggtggcggt
    25981 tggtttacac cagtcatcaa ctatccagaa gtagctattt taggtgtagg aacgattgca
    26041 caacaaccaa tcgtaaatgc tgaaggcgaa atcgttgtcg gtcgtgtgat gaaactgtca
    26101 ttaagtttcg atcaccgtat cgtagatgga gcaactgcgc aacaagcaat gaataatatc
    26161 aaacgtcttt tagctgatcc agagctatta atgatggaag gatgataaac aaatggtagt
    26221 tggagatttt gctgtagaat tagatacagt tgtaattggt gcgggacctg gtggctatgt
    26281 agctgccatc cgcgctgctg aaatgggaca aaaagtagca atcattgaaa gagagtatat
    26341 tggcggtgtt tgtttgaatg tcggctgtat tccttcaaaa gcactgatag ctgcaggaca
    26401 ccattatcaa gagtcactag attctgaaat gtttggagta actagtgaaa acgtcaaact
    26461 agattttgca aaaacacaag aatggaaaga aaacaaagtt gtccatacgt tgacttctgg
    26521 tgttggtttc ctattaaaaa aacataaggt tgaaacaatc gaaggagaag cgttcttcgt
    26581 agatgatcac acactacgcg tcattcatcc agattcagct caaacttatt cgttcaataa
    26641 cgcaatcatc gcaacaggtt cccgtccaat cgagattcca ggatttaaat tcggaggacg
    26701 tgtgttagat tctactggcg gattagcatt gaaagaagta cctaagaaat ttgtgatcat
    26761 cggtggcggt gtgattggtg ccgaattggg cggagcgtac gcaaacttag gagcagaaat
    26821 aacgattctt gaaggctcac cacaaatttt accaacttac gaaaaagatc tagtaaaatt
    26881 agtagaagat gatttcaaga aaaaaggcgt aacagtcgta acgaatgcaa tggcgaaaga
    26941 agcagtcgat aacggtgaca gcgtgacagt gaaatatgca gtggacggga aagaagaatc
    27001 cgttactgct gattatgtga tggtcactgt aggtcgtcgt ccgaatacag atgatatggg
    27061 cttagaacaa gcgggtgtcg aagtaggaga acgaggattg atcacggtgg ataaacaagg
    27121 acgtacgaac gtacctaaca tctatgccat cggtgatatc gttccagggg cggcattagc
    27181 acacaaagcc agttatgaag caaaaatcgc tgctgaagca atctctggca aaaaagtagc
    27241 tgtagattat aaagcaatgc cggcagtagc cttcactgat cctgaattgg cttctgtagg
    27301 tatgacgatc aaagaagcaa aagatgctgg tatcgaagca aaagcatata aattcccatt
    27361 ttcagggaac ggtcgggcat tatcgcttgg taaaacagaa ggattcatcc gtctagttac
    27421 gacgatcgaa gataacgtgc ttattggcgc gcaaatcggt ggagtcggcg caagtgatat
    27481 ggtctctgaa ttagctttag caatcgaatc aggaatgaat gctgaagata tcgccttgac
    27541 gattcatcct catccatctc ttggcgaaat cgtcatggat gcttcggaat tagcattagg
    27601 tttaccaatc catatttgat ttgaataata caggcgaaac gcaactgaaa aatcagttgc
    27661 gttttgtttt ttagaattat caataatatt attatgtaaa cttaaaaata tgacaacttc
    27721 ttggtttatt accacttcct atgctacact gaagaggaat atgaaatagg agagttgaag
    27781 atgcaagaat atcagtatcc acttgattta gattggacaa cagacgaaat ggtcattgta
    27841 atgaagatgt gggaagatct ggaacgtgcg aatgaaaaag ggataaacaa tgaagctttt
    27901 ttgaagactt atcagcagtt taaaactgtt gtcaaatcca ttggtgagga aaaacgattg
    27961 ggacgggaat ttgaaaaagc ttccggttat tctttatatc gtacagtgca gcaagcaaaa
    28021 aaaaatccaa acaaaacttt gagaatgaac gggtgagata aatggaacag atgattgatc
    28081 atatcaagtc ctggcttgaa gaagctggtg agaaaatcaa atcttctcaa caaacattaa
    28141 caatctcgca aaaatcaaat cgaacggatc tagtaacaaa tgtcgatcga aacatccaga
    28201 attttctaat cgaaaaaatc cattcctttg atccagaggc gaaaatattg gcagaagaag
    28261 aagggtgtaa tcaattatcg gatctcgatg gaagagtctt tatcatcgat cctattgatg
    28321 gcaccttgaa ttttgtgatt gaaggggaaa atttttgtat tatgctcgct gtttatgaat
    28381 ccggcgttgg aaagcttggt ttcatttatg acgtgatgag aaatgaactt ttttggggtg
    28441 gaaaaggaat aggcgtgtat aaaaatgatg agccattgcc tagaccagca aataaatcct
    28501 tatcggaagg cttacttggt gtgaatagtt atcttttcgg tcataataaa tatggaatcc
    28561 gagaaattgc ggaaagcagc atggggacac gtatgtacgg atgcgctggt cttgaactag
    28621 tggcaatctt gaaaggaaac cacattgctt atatttcaaa ccttagccct tgggattatg
    28681 cagcgggaaa tgtcttattg gaagagtttg gtatggtaac aagcggtctt tttggtactc
    28741 ctttgacatt tgaaaaaagg gaatactatt tagcagggac ttcttgtgca tatgcagaaa
    28801 tcgcagacct tttgaaaaaa gaaggctgaa aaaaaggtag aaatcattca caaaaatgat
    28861 aaaaacgagc aaaagattaa ttttctcgaa aaaatgaaaa cattttacta tatttcatga
    28921 aaaaagtctg ctataattag cagtcgagta aaatttgccc tgtcataaag aaccaataaa
    28981 aaaggagtta gttacattga attatagaaa cgatatccgt aacgtcgcaa ttattgccca
    29041 cgttgaccac ggaaaaacaa cattagttga tgaactatta aaacaatcag atacattaga
    29101 cgcacatacg caattgcaag aacgtgcgat ggactcaaat gcgctagaaa aagaacgtgg
    29161 tatcacgatt ttagccaaaa acacagctgt agactataaa ggtattcgcg taaacatcat
    29221 ggatactcca ggacacgcgg acttcggtgg cgaagtagaa cggatcatga aaatggttga
    29281 cggcgttgtg ttggtcgtag atgcgtatga aggaacaatg cctcaaacac gttttgtatt
    29341 gaaaaaagca ctggaacaac atatcacacc aatcgttgtc gtaaacaaga ttgacaaacc
    29401 ttctgctcgt cctgagcatg tcgtagatga agttcttgaa ctattcatcg aattaggagc
    29461 agatgatgat cagttagact ttcctgtgat ttatgcatct gcactaaatg gtacgtcaag
    29521 cttgtcagat gatccagcag atcaagaacc aaccatggca cctatttttg atacaattat
    29581 tgaaaaaatc cctgctcctg tcgataacag cgacgaacca ttacaattcc aagtttcatt
    29641 acttgactac aacgattatg taggtcgtat cggaatcgga cgtgtattta gaggaacaat
    29701 caaagtagga gaccaagtag cattaattaa attagacgga acagttaaga aattccgtgt
    29761 aacgaaatta tttggtttct ttggactaaa acgtctagag attcaagaag caaaagctgg
    29821 agacttgatt gctgtctcag gtatggaaga tatctttgtc ggtgaaacag ttactcctgt
    29881 ggatcatcaa gatgcgttac ctatccttca cattgacgaa ccaacattgc aaatgacttt
    29941 cttagttaat aattcaccat ttgccggtcg agaaggtaaa ttcgtaaccg ctcgtaagat
    30001 tgaagaacgt ttgatggcag aacttcaaac agatgtatcc ttacgagttg aaccaacaaa
    30061 ctctccagat gcatggacag tttctggtcg tggagaattg catttgtcta tcttgattga
    30121 aaacatgcgc cgtgagggat acgaattgca agtatctcgt ccagaagtta ttgaaaaaga
    30181 aatcgacggt gtaaaatgcg aaccgtttga acgcgtgcaa atcgatacac cagaagaata
    30241 tatgggtagt gttatcgagt ccttgagctt acgtaaaggt gaaatgcaag atatggtcca
    30301 tacaggaaac ggacaaatcc gtttgacatt cttaacacct gcccgcggtt tgattggtta
    30361 ttcaactgaa ttcctttcaa tgacacgtgg ttacggaatc atgaaccata cttttgacca
    30421 atatctgcca atgcttcctg gtcaaattgg cggacgtcac caaggggcgc ttgtttcgat
    30481 cgatacagga aaagcaacga cttattctat catgagtatc gaagaacgcg gtacagtctt
    30541 cgtagaacca ggtacagaag tatatgaagg aatgattatc ggggaaaaca gccgtgataa
    30601 tgatttgact gtcaacatca cgaaagcaaa acaaatgaca aacgtacggt ctgctactaa
    30661 agaccaaaca tctgtcatca aaaaaccaaa acaattgacg ttggaagaat ctttggaatt
    30721 cttgaatgat gatgaatatt gcgaagtaac accagaaagc attcgcttga gaaaacaaat
    30781 tttagagaaa aatgctcgtg aaaaagcatc taaaaagaaa aaataatctg taaaaggatg
    30841 aagagagaaa ccagcttgtt ataaaaaata gctggtttct ttttttgtcc aactcttttc
    30901 tgtggagaag tcatctttgt caatggacat aaattcagaa aatacgccga aagacgttta
    30961 aaagactttt aaaaaactta tttctagaga gtttttgttt cataataaga taatgtttca
    31021 cgaaaggaga tcaacttgtc cttcgaagaa aatcgaagta aaatggcaag agaaaggagc
    31081 gggaagatga acgacttatt aaaacaatac tttggctatg atgagtttag acctggtcaa
    31141 aaagaaatta tccagaaagt cattgatcag gaaaatgtat taggaatcat gcctaccgga
    31201 agtgggaaat caatctgtta ccagcttcca gctttactat tagatgggct tactgtggtt
    31261 gtgtcccctt taatctcttt gatgaaggat caggtggacg cggcgaatca attaggtata
    31321 cccgccactt ttatcaatag ctcgttagat ggttacgaaa cagctagacg cttccaagaa
    31381 atcgatagac agcagtaccg tttactttat atcgctcctg aacgttttat catgccggat
    31441 tttatccaag caatgaagcg atggaacgtc cgtatgatcg ctattgacga ggcacattgt
    31501 atctctcagt gggggcatga tttccgccca agttatttac agatggcgaa ccagctagat
    31561 caactgccaa atcgtcctgt aattgttgct ttgacagcta cagcgactgt gcaagtagct
    31621 gctgacataa aaagactttt aaaaattcca gaaaacaacc atattcagac tggttttgaa
    31681 cgagaaaatc tacgatttca agtaattaaa gatcaaaaaa aagaacaata tctaatcgaa
    31741 tatttaaaga tcaacaaaaa tcaatctggt atcatttatg cagcaacgag aaaagaagta
    31801 gaccgtctct atcatttgct taaaaaattc gatttttccg tgggtagata tcatggtggt
    31861 ctaaatgaga atgagcgaac agaaatgcaa gaagcctttt tatacgatcg cttgcagctg
    31921 attgtagcga caaatgcatt tggtatgggt ataaataaaa gcaatgtccg ttttgttatc
    31981 cattatcaaa ttcctggctc tctagaagca tactatcagg aagctggtcg ggcgggaaga
    32041 gatggtcttt ccagtgaagc catcctgctt ttctctcctc aagatatcca agtccaaaag
    32101 ttttttatcc agcagtccca aagagaagaa gggcaaaagc aaaaagaata tgaaaaatta
    32161 aaagcgatga cagaatacgt gtatattgag tcctgtcttc agcaatacat cctaaactat
    32221 tttggcgaga caagcagtcc gtgcaaccgt tgtggtaact gtttagatga cagagaaata
    32281 gtagaagtaa cgacagaagc acagatggtt ctttcttgct taaaacgtat gggtgagaac
    32341 tatggtaaac aaatgctgat gaaagtctta gcaggatcga aagaacaaaa gctgcaagct
    32401 ttaggttttg gacatttatc tacctatgga ctgatgaaaa accaatcgca aaaagaaacg
    32461 atgcagttaa ttgaatattt gatatcgaat ggctatcttc tgaccatcaa tggtgagtat
    32521 cctgttttga aagtaacaga aagagggata caagtattga aaggacaaga atctgtttat
    32581 cgaaaggaac ccaagaaagt ccagcagcta tcggatgaag aaaccgatac actatttgaa
    32641 gtgctgcggg aactgcgaac ggatctagca tcagaagcag gcgttccccc gtatgtcgta
    32701 ttttcagatt ccacattgaa agaaatgagc cgaatacgtc catcaagcag attggagatg
    32761 cttcagataa aaggagttgg ccaaagcaag cttgataaat atggcgaagc ctttttatct
    32821 cggataaaaa atgcggactc taacgttctg gataagtaga aagaacgaat acgtaaaaat
    32881 gaataaaaac aactggttaa atctgttttt tttgctcctt atgaaaatca aatatgttat
    32941 aattaaggaa ttagaatggg aggtaagcac agatggattc tttgtcgaaa aaacttgcga
    33001 tccaagtatt aaatgatgaa gcagaacgga tcaaacgatt aatccgaaat caaaacaaca
    33061 gtctctgtat ctcacaatgc aaagccttcg aagaagtggt agatacacag atgtacggct
    33121 tttcccggca agtcggtttt gctgtccgta taggagtaat cggaaagaac gagggccagc
    33181 tgatgttaag tgagctagaa cgagaattga atcagttata tagtgaggtt taccaagaaa
    33241 tttatgataa aaaagaagtt ggcaaggagg aatagacttg cctaagaaag tgaaaaaaag
    33301 gcatctatta gactatagca tcttgattcc ctatttaatt ttatgtgtat tgggattgat
    33361 catggtatat agttctacgt cgtatctatt attagaaaac ggacaaaatc cgtccgcatc
    33421 agtcattaac cagagtattt tttgggtgct tagcttgatt gctatcgcac tactttataa
    33481 aatgaaaaca gatgttttga aaaaccagcg tttgatcatg gcagccatcg cagttttgac
    33541 gattttgctg ctgattgttg tgttttttgg taaggagatc aatggggcga aaggttggct
    33601 gcaaattgca ggattctcga tccaacctgc cgaataccta aaaatcattt ctatctggta
    33661 cctgtctctg actttatcta aaagacaaaa cagtgtacaa aaagattttc ttgggactgt
    33721 gaaacgtcca cttgcaatgg ttatcggact tactgcatta gtcgctattc ttcccgattt
    33781 tggtaatgcg gcagttatct ttttgattat tttggttctg cttttagcca gcggagtcaa
    33841 ttatgtgtat actttgattg taggagtagg aggcttttgt ctaagtactt ttacgatctg
    33901 gctgatcaat atcacaaacg gaaagatctt acccggacgt ttacagtata tctataatcg
    33961 atttgctatt taccaaaatc ctttctcaga cgaattaaat aaagggcatc aactagtcaa
    34021 cggctactat gccatgttca acggaggact atttggcagg ggattaggta acagtatcca
    34081 gaaaaaagga tttttacaag aagcacaaac cgattttatt tatgcaatcg tagtggaaga
    34141 actgggagtg attatgggga ttttgatatt agcgttgcta ttctttatga ttgtgcgcat
    34201 tgtccttgta ggtatccgtt caaaagatcc gttcaattcg cttttgtgca ttgggatcgg
    34261 cgcaatgttt ttgatccaag tatttgtcaa tcttggaggg attactggcg ttattccttt
    34321 gacagggatc acgttcccat ttttaagtca aggaggctcc agtttgctga tgctttcgat
    34381 ttgtgtcggc tttgtcctca acatcagtgc ggatgaaaaa cgcaagagct taggtttata
    34441 ataaaaaaag atgtaggagt ggttcgatga aaaaagtttt agttgctaac aggggtgaaa
    34501 tcgctgtacg tgtatttcgt gcttgtacag aattaggaat taaaactgtt ggaatctatg
    34561 cagaagaaga tgaatattcc gtacaccgtt tcaaagcgga tgaagcatat ttagtaggac
    34621 aaggaaaaaa accgattgat gcgtatctag atattgaagg gatcatttct atcgcgaaag
    34681 aatgcggagc tgacgctatt catccgggtt acggtctttt gtcggaaaac ttgaattttg
    34741 cgcaacggtg cgaagaagaa ggcatcattt ttgttggacc caaactgcat catttagata
    34801 tttttggaga taaaatcaaa gcaaaagctg cagctatcga agcggggatt gcttcgattc
    34861 caggcacaga tggaccaatc gcttctatag acgatgcctt ggagtttgcg aaacaatacg
    34921 gttatccgat tatgatcaag gcagctctag gcggcggcgg acgcggcatg cgtgtagctc
    34981 acgatgaaaa gagtgcaaga gagggctacg aacgtgcgaa aagcgaagca aaagcagcct
    35041 ttggtagtga tgaagtgtat gttgaaaaat acatcgctaa tcctaagcat atcgaagtac
    35101 agattctcgg ggatacgcat ggaaacgtca tccatctttt tgaaagagat tgttctgttc
    35161 agcgccgtca ccaaaaagta gtagaagtag cgccttgtgt ctctatgaat gaacagcaac
    35221 gtcaaaaaat ctgccaggca gctgtccaat tgatgaaata tgtcgggtat gtcaacgcag
    35281 gtacagtaga atttcttgtt gaaggagatg atttttactt tatcgaagtc aaccctcgcg
    35341 tccaagtgga acatacgatc acagaaatga ttacagatat tgatatcgta acaacgcaac
    35401 tgttgattgc tcaaggtttg gatctgcata aagaaatcgg attgcctcag caagaaggaa
    35461 ttaaattaaa tggttcagcc attcaatgcc gaatcactac agaagacccg ttgaataact
    35521 ttttaccaga cacagggaaa atcgatacgt atcgttcacc tggtggtttt ggcgtacgtc
    35581 tggatgtcgg aaatgcttac gcgggctatg tagtgacacc gtattttgat tcattgctag
    35641 taaaagtatg tacgcatggt gctacctttg aaacagccat tcagaaaatg gaacgttgct
    35701 tgaaagaatt tagaatccgt ggagtgaaaa caaacattcc atttatgctc aatgtcatca
    35761 cacatcctga attccaatca ggaaatgcaa aaacaacctt cattgatagt acagcaacat
    35821 tatttgaatt tcctcgacta cgagaccgcg gaaacaaaac gatgaaatat atcggtgaaa
    35881 ttactgtgaa tggttttcca ggtatcgaaa gtggcgaaaa accattttat gaagaaccac
    35941 gtatgcctaa agatctaatc acaagaactg attacgtaac ggctaagaat gtattagatg
    36001 cgaagggtgc cgatgctttg gtcgaatgga tcaaaggaca ggaaaatttg ttgttgacgg
    36061 atacgacatt tagagacgcc catcaaagtc tgctagcaac tcgtgttcgg acaaaggatt
    36121 tcaagcagat tgcacgtttg actggagaag gtcttcctga actcttttca agtgagatgt
    36181 gggggggagc aacatttgat gtggcttacc gctttttgaa tgaagatcct tggcaacggt
    36241 tgagaaaaat tcgttcttta atgcctaata cgttgctgca aatgcttttc agaggttcaa
    36301 atgccgttgg gtattctaat tatcctgaca atgtattggt ggaatttgtc aaagaagcag
    36361 cagcgcaagg aatcgatgta tttcgaatct ttgatagttt gaactggact ccacaaatgg
    36421 aaaaaagtat ccaagctgta cgggatactg gtaaaatcgc agaggcagct atttgctata
    36481 ctggcgatat caacgatcca agtcgggcaa aatacaatgt tcagtattat aaagacatgg
    36541 caaaggaact agaacaactg ggtgcgcata tcattgcaat caaagatatg gctgggctgc
    36601 ttaaaccgca ggcagcttat cgtttgatta gtgaattgaa agaaacgaca gatctgccaa
    36661 tccatctgca tactcatgat accagcggga atggaatcat cacttattct gctgcttcaa
    36721 aagcaggagt agacatcgtt gacgtggcaa tgagtgcaat gagtgggaat actagtcagc
    36781 ctagcatgag cagcttgtat tatgcactgg tcaatggtcc acggctgcca gaaatcacga
    36841 ttgaaaatgc acagaaatta aaccattatt gggaagatgt gcgcatgtat tacaaaccgt
    36901 ttgaaaacgg cttgaatgct cctgaaacag aagtatatat gcatgaaatg ccaggaggac
    36961 agtattcgaa tctacagcaa caggcaaaag ctgtcggatt aggtcatcgc tgggatgaaa
    37021 tcaaacaaat gtatcatact gtcaacttga tgtttggcga tatcgttaaa gtaactccat
    37081 catctaaagt agttggagat atggcactat ttatggtcca aaatgatctt acagaagaag
    37141 atatttatga aaaaggcgaa acactaagtt tccctgaatc tgttgtgact ttcttccaag
    37201 gagaactagg gcaacccgtt ggcggatttc ctaaaaaatt gcaaaagatt attcttaaag
    37261 gcagacctgc attgaatgaa cgtccaggtc ttttggctga atcagtcgac ttcaacgaag
    37321 taaaaaaaga attggcagaa aaaattggct atgaaccaaa acaagacgaa gtattaagct
    37381 atttgatgta tccgcaagtc tttttggact atcaaaaagc atataatcaa tttggcgatg
    37441 ttacattact ggacacacca acatttttcc aaggtatacg tttgggtgaa acaatcaatg
    37501 tacaaatcga acgcggaaaa acgcttatca ttcgtttaga tgaaatcgga gaacccgata
    37561 tcgaagggaa tcgcgtactg ttcttcaatt tgaatggaca aagacgtgaa atcactatca
    37621 aagataattc gatcatcagt acggtccaaa cccgattgaa agctgaaccg acaaaccgag
    37681 aacaaattgg agcaacgatg tctggttctg ttttggatgt attagtgaaa aaaggcgata
    37741 acgttaaaaa aggtgatacg ttgatgatta ctgaagcgat gaaaatggaa acggccatcg
    37801 aagcacgttt tgacggagaa gtcgcacatg tatatgtgtc ttcaggagac actatttcgc
    37861 ctggtgacct tttgattgaa gtgactgaga aataataagc gaaaggtggg gaaacatgaa
    37921 aagactgggt ttgttttggc tgattctgct attagttatt gtggcattct acatggaacc
    37981 agtcttgttt ccgccaaaac ttgaagtgaa ggaaaactct gatcaacaaa tatcaaacca
    38041 aatgacaacg tggaaatacc aggagcttaa agcaactggt ttttctactt atatcggaaa
    38101 accgatcgat cagctggaaa aggattttgg ccaagcctat gatcgattaa ctagcggatt
    38161 tggttttgaa acaagatatt atgaaaataa agctagtcat ctcacatttg aagcgaacat
    38221 ccaagacagc agagtcacaa ctgttaaagt actgcaaaca gggcctgctg atatcgcgcc
    38281 gttttattta gggatgacga tgcaagatct tacgaaaatc actactatct atactaattt
    38341 cacttttgag taccatgata cagaagttgg aatcgaacta atggaagagg atatgaatta
    38401 tcgtccgcta attgcttttg ataacgacac ttttgctatt ttattttttg atcaaacaac
    38461 aaacggccta ttttcagttg tgtatttaga taaagatagt cttttgaaac tattgccata
    38521 tcaagtgttt ggtgaaggcc ttccacatta tcaagcagat aaatcggctg attgggaatc
    38581 aatcaatcgc gttaaagaac gaaaaagtac gactttatta aatatgctta gaagagaaga
    38641 cgaacttccc atatacaatc aaacacttgc ctttcaaaac cacagtcagc tgctactaca
    38701 tgattttttg acaaggcctg aggaaatatt gtcagaggaa cgtatggctg attggcaaaa
    38761 atcgctaggc agtgctcaaa cgacagtgaa attctcactg acgaatgatg aattggagaa
    38821 gttaatcagt aaacaaaaat tagaaaatac tagtgggcta tttattcatc cggcgattga
    38881 cgctactttt tcgtttttat attggtacag cgatccttac aaccatgacc gtttcatgga
    38941 cccgggaacg gattctgtag gcattgcttt ttctaaagaa aatatgttag tcttattgca
    39001 agaagaaaac ggagaaagta gtgatagcag tgatcgttaa cgaagagctg tttcaactgg
    39061 aggatcaatg cctccaagtc gctgaaatga taaaaaagag taaaacgatg gcttcttatc
    39121 ttgaacataa acagtcaatg gaagaatctg caaaagtttg ccagttgaaa gaagcgttct
    39181 tgtctaagaa agaagcattt gaaaatgtag cggcatatgg tgagtatgca cctgattatc
    39241 gtgaaaaaaa acgagctgtt cgaaaagcaa aaagacagct tgatatggat gagcatgttg
    39301 cagctttccg atatagtgaa acacagctgc aaagtctgtt ggatcaaatt gtccaagtac
    39361 ttgccgaaga agtttctaat gaaatcaaag tagatgcagg tagcccgttt tttgaaacga
    39421 aaaaacactt tggatgcgga ggtaattgtc atgcaattag acaatagtga atttgaaata
    39481 acaaaacgac gcgggctgat cgtatgggtc tacagcttaa aacaattaaa gaatctgaaa
    39541 aagttcggct tgttgcatta tgtctcaaga agaatgaaat atgtcgtgct ttacttaaat
    39601 gaagagacat tggaagaaac agaagaacga atccgtaaat tacattttgt tcgaaacgta
    39661 gaacgttctt accgaccgga tatcgaaatg aattttgccg aaaaaatagg aaacaaaaca
    39721 gtattaaaag acgaaggttt cgaaattgaa gagctaaata cagaaatccg attagctgaa
    39781 catctttaaa aatctcttat gagcagtttt taaataaaaa ataacttgga taacgatcga
    39841 tttttgatca ttgtccaagt atttttttgg tatactagta ccagtaccat ttctaaggag
    39901 tgagtagatg cggataatat caggaaatta tggcgggcgc cgtttaaaaa gtttagcagg
    39961 ggcgaacacc agaccaacta cagataaagt aaaagaatcc atattcaata tgattggacc
    40021 atattttgat ggagagacag tattggatct gtttgcagga agcggtggtt tagctatcga
    40081 agcagtatct agaggctgca gtcatgcggt atgcgtagac aaaaattacc aagcgctaaa
    40141 aatcatcaaa gaaaacatcg aaattacgaa agaaccagag aaatttacca caataaaact
    40201 tgatgcggac aaagcaatcg aacggctagc agcggagaaa caacaattcg actacttgtt
    40261 tcttgatccg ccatatgcaa aacaaaaaat aatcgatcaa atccataaaa tggaagaact
    40321 gggacttttt tctaaaaccg cagttatcgt atgcgaaacg gataaaacag tcgaattgcc
    40381 agaaactgtg gctggattcc atcaaataag aaaacagatt tatggcataa cggccgtaac
    40441 tatctataga aaagagggaa atcaatgaaa cgtgcattat tcccaggaag ttttgatcct
    40501 tttacaaaag gtcacttaga tacggtggaa cgtgcggcta aattattcga tgaggtagta
    40561 attggtgttt ttatcaatac ttcaaagaaa agtttgtttc ccccagaaga aagaatgaca
    40621 ttgatcacaa aagctgtttc tcatcttcct aatgtaaaag tcatgcatca agaaaatcaa
    40681 ttgacggtag aaacagcaaa agagattggt gcagatgcgt tgatcagagg catacgaagt
    40741 atcaaagact ttgaatatga acgagagatt gctcagatga atcatcatct atatgatgaa
    40801 ttggaaactg tcttcttatt agcaaaacca gaatatagtc acgtgagttc cagtatttta
    40861 aaagaagtac tgcactttgg cggagatgtg agcagctact taccgccagt gatcaacgaa
    40921 gctttagctg gaaagcgtga atcaaatgaa agctaaacaa tggttgaaaa cagtactgat
    40981 tatcatattt ggaatgttga tcattggatt tgtagtagtt cccttgccgt attatgtaga
    41041 atcaccggga gccacgatcg atctgaaaga actaattacg gtcaatgata aggaagataa
    41101 aggttctggg tctttctctc ttacctctgt tggtatcagg caagctacag gtttcttagc
    41161 tttaaaagct aaaatgaccc cttttgaaga attgatcagt gaagaagaat tgactggtgg
    41221 ggcgacaaat gaagaatata atcaaatgca acagtattat atggattctt cccaaaatgc
    41281 agcaatcgaa caagcgttaa aacttgcaga caagccttat aaaatggaat tcaaaggcgt
    41341 atatgtcatg agtgtagaga aaacatcgaa tttctttggc aagctttcag ttggagatac
    41401 agtaacaaaa gtagacggga aaagctttca aagtacagaa gcatttatgg attatgtaaa
    41461 aagccaaaaa gttggacaaa caatcgaaat cagttatttg cataatggtg aagaaaaaac
    41521 agcttctggt gatctgatcg agttaccaac agataaaaag gcaggaatag gtatcacgct
    41581 tactgaccat accgagatcg aatcagatac agatgttcgt attaattctg gctctatcgg
    41641 tgggccatca gcgggattga tgttcacttt agaaatctat gaacaagtca ctggtaaaaa
    41701 cttgcgacat ggtaagcaaa ttgcaggaac tggaacgatc aacagtgaag gagaagttgg
    41761 ccgaatcgga ggaatcgata aaaaagtggc aagtgcggat aaagctggcg tagagatttt
    41821 ctttgcacca gatgatacga tttcagacga tgttaagaaa gaatatcctg aaataaaaac
    41881 caactacgaa gaagcaaaag ctgccgctaa aaaattgaat tcctcaatga aaatcgtacc
    41941 agtaaaaaca gtacaagaag cgcttgacta tctagagaaa atgaaatgaa actaaataat
    42001 taaagagggg ttgtgacacg agttctgtca caacccctct ttttcgtcaa aaatcttcac
    42061 gataaaaata aaaacaaaag aaaaaattga cgtaattaga tgttgaaggg acgattttgc
    42121 cagagctcac tgtttcttca attttaaatg aaaggagtaa tgaatgattt ttactggtca
    42181 ttctttgaaa aaagaacaat gatagaaaaa atcagcaaga aaatactagc ttcacccaaa
    42241 ttgatcattt ggatgtctat gatagttctt atcttttgta tcgcaagtgg ttttttatta
    42301 tcaaatttgc tattttatag agaaacagaa gagactgctg caaaagaaat actttcttcg
    42361 tttagttcca gtggagaaga tccatcagct gaaggaaacg atgtagaaga ggatgctcgt
    42421 ccaaaggtca tgtacaccga tatcaaagga agtgtgaagg agcctggtat ctattccttt
    42481 tcttcagaag aacgggtcta tgatgtacta aagcgtgcag gtggattatt agaagaagcg
    42541 gatagcgatc gaatcaactt ttctgcgaag atagaagatc agcaagtcct atatatccct
    42601 gctgtaggtg aagagctgcc agaacatctt gatcagttgg catcctcgga aggaaaacag
    42661 cctacagcag attccgagcc tcccaaaata aatatcaata cagccactcc atcagaactt
    42721 cagcagattc ctgggatcgg atcagtcaaa gcgcaagaaa tcattcgatt tagagaagaa
    42781 aacggatcct tccagaaagt ggaagacctg caggagattt ctggtatcgg tgaaaagaca
    42841 gttgaaaaac taaaaaactt cgttacaatt aaatagaatg attcaaaatc gactggcacg
    42901 agcaataaat ggacgctaat ggatactaag aataatccaa ttctaagagc ttggcaggac
    42961 gaggaggtag tatttttgga taggaaatag cttgattgtt cgcaaagaaa cattaaaaaa
    43021 accaatgctg aatgtctatc tccaaaataa aatatctggg attcatatta tgaatacggc
    43081 tgtcagcggc aataacagtc aagctctacg agaacgcttt gccaaagatg tacttagcta
    43141 tacagcagat aaagtattta ttctcattgg gacgaatgat ctagcagagc ataaacaact
    43201 ttctaaagaa acgtaccaaa aaatctgcag tggatgatag ttcagctgaa aaaagactat
    43261 agttctgatc atgctttctt tatcacgcca ccagcagtcg atgagacaaa acaaaaatat
    43321 cggaataaca agttgatcaa agaatatatc tctgttctca cagaagtagc aaaacgccat
    43381 gattgtgaag tatcgatttt tatacggaat ttcttgaaca aatggattcg aagaattttt
    43441 ataatgggct aatggatgac ggacttcatt tcggcagtga agggtaggat cttttgtcag
    43501 acaggatgat tcgttatatt taaagaatat tttgtttaag caatgagtac tttttaatca
    43561 acttgaaaaa acggaggaat ataaaacatg ttagaaaaat cttcaaaagc atgcgatacg
    43621 aataaagaaa aagttactga taagtcacat aatgaacgca ttccttggga ccagtacttt
    43681 atggctcagg cagtactgtt atctttacgt agtacgtgta cacgtttaga agtaggagcg
    43741 acactcgttc gagaaaaaag gatcattgct ggcggctata atggagcagt cagtggagat
    43801 gtccactgca tagatgaagg gtgttacgtc gtggatggac actgcttgcg aacgatccat
    43861 gcagagatga atgcactcct acagtgtgca aaacttggta tcccgacgga aggttcagaa
    43921 atctatgtca cacattttcc atgtttggcc tgtacaaagg ctttgttgca agctggtgtg
    43981 aaagggatca attatctcca tgactatcga aatgatcctt atgcccaagc gttgatcgaa
    44041 caattaggtg tatctgtaca ccaagttgct ttagactcac agcatttttc aaaacttcaa
    44101 aatgaattaa caggaaaaca tgtctagcgt tttagaaagg catgttcgaa atcggttgat
    44161 ttttccggca attacgtcga tgattatctg tttgttgtat tacactgaca attggggtta
    44221 tcgtttgctt cttgccggaa tttttttatg gttttcgcta aaagaaggac gttttatcgc
    44281 gtacttgtgt attttgtgta gcataacggt atccatttct tataggctac aagagtattc
    44341 atttttagag gaaaaaacaa catctgcgca aattactgta tttacagata cgattcgtgc
    44401 aaatggtaat tttttgacaa tggaaggaag gttatcagag aataaacaaa agctccagct
    44461 ttcttatcaa gtaaacacag aagcagaatt gaaaacatgg cttaacttcc gaggaaaggg
    44521 aatagaattg actgctgaag gcgagttcat ctctccagca aaaaagcgga atctttataa
    44581 ttttgaccaa gaagcttact tcaggaacca tagaatttct ggacggttcc aaattcaaaa
    44641 gattgaacag atcaccatgc aaaatcactg gcgaaactgg cttcagagaa aaagaggatc
    44701 ttttatctcc tttgtccaat ctgaatttcc gcaaaaaaca agtgtatata ttaattcttt
    44761 attatggggc tataaagatg caggattcag agaaccagaa gaaattttta gggagagtgg
    44821 tttgctgcat ctgttcagtc tatcgggtct acacatccaa ttttatttag ggtggctgta
    44881 ttatttattt cgacgtatcg gatggccact acacgtcagt attattccgt tgagtctact
    44941 aactatctgt tatgtttcta ttgcaggaag ttctatttct gttttgcgtg ctagtttgct
    45001 gtttttattg cgattgctag tcaaattgct gaataagcat tattccacca tggacttatt
    45061 tgcagttgtc ttttggcttc ttttactatt tgatcctcga tttctttttc atgcgggtgg
    45121 acagcttagt tttttcatga gttttttgtt tatcctgatt tcttttgatg gagattggtt
    45181 gaaaaaaacg gcctctcacg tcctgtttac tatgatcacg atgccgttga ttgtatggca
    45241 tttttatgag tggcctattt taggtagtct ttttacatta ctgattgcac cggtattcaa
    45301 gtttcttttt cttcctggtg tgttatttct cacactcttc aatcgctggc tgccggaaac
    45361 tttacttttg ctgatagaag agggattaat cttatttgag cgaataatcg atttttcttc
    45421 agggtcgtct ctcattatag gacgtttgcc ccttgtgcta tctgggatgt atattttcgg
    45481 attactgatt tgtatggata ggttgaaaga acaacctgtg aaacacttgt tttatgttgg
    45541 tcttttttct tttatttttt tgactttgcc atttttacgc ttttcttcaa cgatcgcttt
    45601 tatcgatgtc ggtcaaggag acagtatctt tttgcaaagt ccttttcgaa aagaaacgat
    45661 tttgatcgat actggaggtc agttacactt tgaaagagaa aagtgggcgc aaggtatgat
    45721 acgtcctcct gctgaaagaa atatcgttcc atatctaaaa ggtcagggga taagtgtaat
    45781 cgataaactc atcttgacac acgacgatac ggatcatgtg ggagaattac ccacgttatc
    45841 tcagcatttt actatcaaaa aaatttatat cggttggggc gctggacgaa atgagccatt
    45901 acaaaggcat ttatctgaag ctcaaaaaaa tggtactgag atcattgaaa tcaaacatgg
    45961 ggacaggatc agcggttatt atgatcttta tgtattgact ccttttgaaa agggagaggg
    46021 aagaaatgaa gattcgattg gtctatggat ggaatataac aataggcgct ttttattttt
    46081 aggagatttg aatcaagcaa tggaaaaaca attgcttttg atttatccta atctgaaagc
    46141 cgatgtagtc aaattaggtc atcacggtag ccgtacgtca agtaacacag attttctttc
    46201 ccatattgaa gcaaaacatg ggattatatc ctgcggcgta aataatcgtt atggtcaccc
    46261 gcacagcgaa gtattggaga cactagaaga aaatcagata aaaacatggc gaacggatca
    46321 acaaggaatg atcacctatc gctggcatcc agtttttcat cctcatgggc tagtggaaac
    46381 aatgatagac tagtaattct catctgctca tggtaggata atttttgaaa gtggtgagta
    46441 atttgagtat acaagcagag ttacaaaaaa tacgtaaaga aaaacttgca tcttgttact
    46501 tgattttagg aacagaaaag tttctccaag atcaagttcg gacagaaatt gtcaaaaaaa
    46561 tccaattaga gggcgaagat gacttaaatt ttttaagttt tgacatggaa aatgcaagtt
    46621 tagatgaggt agtagctgaa gcagagacgt tgccgttttt cggagatcaa cggttagtat
    46681 tcgtagagaa tccttatttt ttaacaggtg aaaaagtaaa caatggaatc gaacaaaata
    46741 ctgatttatt gacggcttat ttgaaagaac cattagaatc gactgtatta gtattctttg
    46801 cgccatatga aaagttggat gaacggaaaa aagtaacgaa acaattgaaa aaaacagcaa
    46861 ttatcatcaa tgtccaacag ttgaatgaaa aagaagtacg tcaatatctg atgaatacct
    46921 tagagaattc ctcaatcaaa atggaacgaa gtgcaattga tttattcttg aggttgaccg
    46981 acttagattt atccaaaatg atgagagagt tacagaaatt ggtcctttat ggacaaaatc
    47041 aacagcaaat cacggtaaga gaagtagaag aattagttcc taaaacgtta gaacataata
    47101 tttttgacat gacccaatat attttaaaag ggaagacgga acaagctttg cgtttatacg
    47161 aagatttagt tatgcaaggg gaagaaacaa tcaaaattaa tgcgattttg ctttctcaat
    47221 tacgtttatt tttacagaca aaatttcttg taaagattgg ttatcagcag gcaaatattg
    47281 ctgaaacatt gaaaatccat ccatatcgtg taaaacttgc catgcaggaa gtaaaaaaat
    47341 tcgatgaaca tttgctggta cagttgtttg atcaattagt tgagatggat tatcaaatca
    47401 aaacgggaca aatcgaaaaa gaattaagtt ttcaactatt cgttttgcgc acagggcagt
    47461 tgttaagaaa tcgataagaa gaaagacgag ggcaaagcat gcgggaaggt cgtttgtttt
    47521 tgtccttttt ttgacaaata aaaaaatcgg ccgaagccga ttcttttatt tagcgatttt
    47581 tttgcttaaa cgagatttgt cgcggttagc tttgtttttg tggattagac cttttgattc
    47641 agccatatca atagctttaa cagcttcttt atataacgca tctacgttgt cagcacctga
    47701 agctacagca tcttcaaatt ttttgattgc agtacgcata gcgcttgttt gagatgaatt
    47761 tttcacatta gcgttttcgc ttgtacgaac acgtttgatt gcagattcaa tgtttggcat
    47821 tagtttcacc tccgataact taagttctta cgcgtgttac cgcataattc aacattcatt
    47881 attataccta aaggcagcgt gctttgcaat atgaacatga caagtttttt tccttaacag
    47941 tcttttatta ggattttttt tcagtaaaaa cggatagtac aagctgaaaa aaatcaaaaa
    48001 gacgctattg tgggaggcaa tagcgtcaaa ccaaactaga tttttattta tctatttaaa
    48061 caaagtttga atgggaatgt ttggtactaa gttattatat aacaaagaga accgtttgca
    48121 aagttttttt tgaaaaggga tacgattccc tgttgttctt tgtttttcta ttaagaaact
    48181 gcagttattt tataaccagt atcctttgat cagtaaaggc attctggtgt accgattatt
    48241 ttaatgtatt catcgatatt ttttatcaac atttttttta tgaccaactg gttccgacgg
    48301 ttccatttgc cacttacttc cagcacatat ttatttaggg gcaaacgtaa gatatatttt
    48361 gccaaatagc gatctttaag caggccattg atcacacggt cctcaagctg caagttgatc
    48421 ccagtcagca tcattggctg ttcttgtaaa acgtacgtat caattaaaag accttcaaaa
    48481 gtttccacaa gcgtcttcct ttcttttcaa agtaacctga aaacaggcac taattcttcc
    48541 attttgatat tcgttgaagt ggatgcgatc attctttctt ttgtccactg taatctagta
    48601 taacaaaaaa gagcagaaga gccaaaaaga aaaaagagca tcgaattcta taggaagtct
    48661 cattggcacg gcctaaatta ttacaaaaca ttgattaaaa aaagcccctg catcgcagga
    48721 gcatccccta tatcgtttta ataggtcaat caaaaagcag tatcaaacac attttgtatc
    48781 aaatcaggaa ataatagata agctaagccc agaatcaaaa gtaccgctag aagaaaccag
    48841 ccgattgttc ctatgaaata ctttaatttt tcgaacattg tatcacctcg ttgtcagtat
    48901 atcacggatc agtaaattgg gggtgttaag aatctcctaa aatagacaaa atttgttcta
    48961 ttttcctaaa gcaaatactt gtaagataca gtaagtgata ccagaagcaa tgaccggtcc
    49021 ggcggcgatt cctcttaaga agacaacacc taaaatcgtg ccgaaaacaa gtgcaacagt
    49081 gatttgtgga tcagaagcaa tcaatcctac acccttggaa gataagacag ccactagccc
    49141 accgcaaaaa acagctaccc agccaatagg ggatttaaag gcttctaata gatcacgtag
    49201 accgatttct cctgttgcga tagggattag aatagccgct gtgatgatcg tcaccccaat
    49261 attgattcct ttgccttgaa gccaagggaa aaaggaacgg gaggctggta tgagcttcat
    49321 aatcaaaaca aaagctgccg ctatcatcag actttgattt ttagctaaaa ttgctacaag
    49381 taaaataatt cctaaaaaaa gccaactttc catgaaatat attcctcact ttcttcgaaa
    49441 caatcataac atgtttttag aaagttacaa aagtaggaat tcatccttca agacctattc
    49501 ataagctgtt tttttcataa aataaacaaa atcataaaaa aacactttgc ttatttgaca
    49561 gaagacagcc gcgctttgta tagtggacaa gacttacatt tttttgaaga atcagaaaga
    49621 tcaggtaaag gtcttagtaa acgcaagcca ttcagaataa ccaatattgt gcttccttcg
    49681 tgtcctacaa ctccaagagg tagatttaaa aattgcagga agttagaagc aatcaacaag
    49741 agaataacaa gaatcgaaaa aataatattt tgtttgatga tccgccgcat ttttaaagat
    49801 agccgatgac tgtagacaaa tttatttaag tcgcttttca ttaagacgat gtctgcgata
    49861 tccatagcgg catctgtccc ttgccccata gcgacaccta aagaagcggc tgctaaagca
    49921 ggcgcatcat tgactccatc accaaccatc gcatttactt ggtaaagttc attctctttt
    49981 ttgactagtt gtgttttttc ttccggagta cagcttgcat agtaatgttc aatccctact
    50041 tgttcagcaa tggcctttgc tgtgccagaa tggtcgccgg ttatcatact tgtatggatg
    50101 ttttgggatt taaagtagga aattacttgt tgggtatttg cttttggaat atctagcaga
    50161 cctaaaacag caattaactg ctgatcttta gaaagataaa tcacggtttt gccttgattt
    50221 tccaaacgtt cgatcatttc cgcaatctct ttgctgatta tcatcgtctc aggatcatat
    50281 gcgtgcttgc caactttcca ttgcgcacct aaataggtag tttgtaatcc aaaaccagta
    50341 atttcttcta ttggtaagta ttggatttct tcaggtattg tgcaatcgaa atattgcaaa
    50401 atcgcatgag caagtggatg cgtcgttttt tgttccatag caacaagaag ttgttgtgcc
    50461 aaatcctttt catcaaagaa aaagctgtca gttacaactg gttttccttt agtgagcgtt
    50521 cctgttttat caaaggcaat cgcttttaat tcagaaagct tttctaaatg gataccgcct
    50581 ttgattaaaa tcccattttt tgcagaattt gaaagagcag ctagagaagc tggtgtagca
    50641 gatgcgacaa gcgcacaagg agaagccaca actaataata ccattcctcg ataaaagctt
    50701 tcatcccaac tccaacctag gaaaaagtgg gggagaagga tcattagagg aacagcaagc
    50761 aaaatcaatt ttacataatt attttcgatt cgttcgataa atgaagctgt ttgagttggc
    50821 gtattctgcg cttcttctac taattggata atcttagcaa agacagtttc ggaactcgtt
    50881 tttgttactc gaatcgtgat gggatttcct tgattcagcg ttcctgcata catctcatcg
    50941 cctaaatatt tttctacagg cacagattca ccgcttaatg cagactcatc gatcgttgcc
    51001 tgtgtgcttt ctaaaaagcc atctatcgga acagctgctc ctttagcgac aaataccaag
    51061 ttatccacct ttagttgaga aacatctact tcttgcattt gtccattttc ttttaataat
    51121 tgggcttttt gtggttgaag attcatcaaa gcagtaattt ctttttgact tttattagtt
    51181 gtgtattcct ctaatgcacc gcttaaacaa aagatgaatg tcaacattgc tccttcaaag
    51241 taattaccga tcaaacaggc accaatagca gctaaagcca tcaataaatc cacattcagt
    51301 gtccgatcat tccacaattc tttcaagcct tccgatgttt gtttatatcc accgatgata
    51361 atggcactga tataaaaaag tggggtgatg gtaagagaga atcggtcgaa gataaaaccg
    51421 aagatcataa ataaaaggca gccgattgtt gctgcaaaag cttttttctg attagtcatc
    51481 gttattagac ctccttgtca aaacaaggat agcataataa aaatgtaatt gataataata
    51541 acagctaaat gagaacgata atggttatca tttggtgatt aagcttgatg ttgtttttat
    51601 tatttataat gattattatt aaagaaaata aaaaaccaga acatttctca aataagagaa
    51661 gtgttctggt tttttctaca ttgcaataaa ccaataacca atcaaaatga taccaaggat
    51721 aatacgatac cagccaaaag cggtaaagtc gtttttcttc aaatagctta gtaagaattt
    51781 gatagctaag acggaaacca caaaagatac gaatgttcct acaagaagaa caacagtttc
    51841 ttccattttg aatgaattcc cttcagctaa atatttgacg atcttcaagc cgcttgcgcc
    51901 aaacatgaca ggaatcccta agaaaaatga aaactctgta gcgacaaaac gcgaagcacc
    51961 aatcaagata gatcctagga tcgttgcgcc agaacgggat gttccgggaa caagtgccaa
    52021 gacttggaat aacccaacaa gaagagctgc ttggtacgta aagtcattca gagaagtcca
    52081 acgaggtgtt ttgtttttgt tgcgtttttc gacaataata aaggcaatac cgtaaacgat
    52141 caaaacaatg gcaactggta agaacttgtg gaaatttgct tctaaccaat catccaaagg
    52201 caaaccaatg atgatggctg gtatgcaagc aacgatcact ttaaaccaaa gtgtccacgt
    52261 atcttttttt tctaccgttg atttacttgg agaaaatggg tttaacttgt gaaaataaag
    52321 aacgacgaca gccaagatcg cccctaattg gataacgaca ttgaacatcg acataaattg
    52381 tgaactggca tccagtttga tgaattcatc tgctaggatc aaatgaccag tactactgat
    52441 gggcagccat tccgttattc cttcaataat accgagaatc actgctttga taatatttgc
    52501 aaaaaacata aattccttcc ttcccaatta agatacatct tatagtatac cgttattttt
    52561 tgagaaaccc aatctgtttt ttaatattaa gaaaaacttg agaaataaag cagataagta
    52621 cacgaagaga aaaaagaaga caaagccctc gttcagactt tgccttttat gattctctgg
    52681 taaaaattaa aatatcccca ttatagaaac gagtcgcgac tatgtgtttt cccgttatcg
    52741 atataagacg tatcattgat gccgtggata gaaaatcgac cgtttgcata agtgaccttt
    52801 gttacgctgc catttaaaag cccagcacga acaggctgtt taggatcgat cagattaagc
    52861 aaaaaggcga tcgtcaaacc atgagatacg actaaaacat ttccaccacc atttttttcg
    52921 cagtgatggg cgatatcttc aaatcctgtc catacacggt ctcgcaatgt ttcataagtt
    52981 tccgcccacc cagcggtatc taagttgtat acagcattag caatttgttc aaaagaaact
    53041 tctgtggtaa acatttcatc atatgtttta aaattcaata cacgagggat cacaccccac
    53101 atttccatgt catatccacc ttcaagagac ccgaaacacc attctctaat ccttttttca
    53161 ataaaataag gaatttcttt tcccttagaa tgttctccta gaataatacg ggcagtttcg
    53221 attgcacgac cactgtcact agaataagca aatgaaaagt cgacattttt taacccgcga
    53281 cctaaataat gaatgccttc ttgtccttgc tttgtgaggg gtgtatcaca ccagccttgt
    53341 actcgttcaa ttgtattgaa catcgttttt ccgtgtcgta cgatataaag tgtagtttct
    53401 gtcattttat tctcctccta agtcgtttta acgattgaaa aaaggatttg tccttttttc
    53461 ctgttcaatc gtcgttgcat cgccatgtcc tggatagact gcaaattcat caggtaatac
    53521 aaacagctgg gtccgaatac tatgaagtaa ttgttctaaa ttccctgtat acagatcggt
    53581 tctaccgata cttcctctaa acagggcgtc gccagaaacg acaaaatcat caaaaataaa
    53641 gctgacgctt cctatagaat gccctggggt agggacgacg cgaaaagaca tgtttcctaa
    53701 agtataagtt ttcatttcaa attcatattc tgcaggcgag acaataacat cagcaatatc
    53761 atcatgtcta ccgagtccag aaagattcaa aatcggatca cctagccatt tttgctctag
    53821 tgggctgaca taaactggga tttgataata cgcgcgtaaa ggttccactg caccgatatg
    53881 atcgtagtga gtatgggtaa gcaaaatcgc taccggctgt tggtttgttt ttttgatttc
    53941 ttgtttgatt cgttccgcat cttctccagg gtcaatgatc aaaagtgctt cttcgttgta
    54001 gaccaaataa cagttttctt caatcattcc tgtttttaat ctttcgatat gcatgtgcat
    54061 tcctccttga tagccaaaac ttctttcatc cattagtata acgcattcat tgctaggaaa
    54121 caaaaacagg tacttatttc tctttactta tctgaattat cagactatat gaaaagtatt
    54181 tggatattct aaagagaagg aaagtaagtt ttcatagcat tttaatatgt ccttgttaca
    54241 ttagattcat ccaaaaatcc aaattctata tcctatatcc ctgcctaatc agcagggtgt
    54301 ttttttatgt ttggaaagag agctcttttt gctaaactag gttttttcag gtaatcttag
    54361 agagaaagag gtggtggaaa tgatcaaaca gtcaaaaata tctgtgcggc acttagtaga
    54421 atttattttg cgacgtggaa gtatcgacaa tcgtaaaaaa agtaaccata ctgcattaga
    54481 aggagcgaga atccaccgca agctccaaaa agatgcggga gaaaactatg aaaaagaagt
    54541 ctttttaaaa acaacagttc aattagatga atattacctg atcgttgaag gccgtgcaga
    54601 cggtattttc aaaagagacg gtatttatta tatagatgaa atcaaaacct ccgaaccacg
    54661 atttgaagat ttagaaccag aacaagtgga acttttcttt catcaagcaa gggtgtatgc
    54721 ctatatctat agtcaagaac atgatttaca agaaataaat ctgcaattga cttacttcca
    54781 gacggcagaa gaagtgatca cgagaaaggt tgaacatcag acaagagagc agttagaaac
    54841 tttttttaaa aagctgactg aagattataa agaatggctt gtttttcaag aaaactggcg
    54901 cactgtacgc aatgcctctt tgatggcttt gaaattcccc catgatgagt ataggaaagg
    54961 tcagcgtgaa ctagcggtag ctgcttataa aacgatccgt acgaaacaga aattgtttgt
    55021 agaagcacct accggaacag gaaaaacaat ctctacgtta ttccccgctt tgaaagctgt
    55081 aggggaagaa gagggagaaa agatctttta tctaacggct aaaacaataa ctagacaagt
    55141 agctgaagat gctatgacag cattaaaaga tacaggagct gaagtaaaaa gtgtcacttt
    55201 gacggcgaaa gacaagatct gctttttaga tgagacgacc tgtaatccag atcagtgccc
    55261 atatgccaat ggttattaca atcggattaa tgaaggttta tgggatcttt tgaatcatga
    55321 gaatcagatc actcgtgaag tcatcgaaac ttatgcaaga aagcatacac tttgtccttt
    55381 cgaactatca ttagatgtca gtatctggtg tgacgtaatc attggggatt acaattattt
    55441 atttgatcca actgtttatt taagacgttt ttttgaagat gaaaaaaacg aggactatct
    55501 gtttttgatt gacgaagcac ataatttggt gaatcgttcc cgagagatgt attcggcaga
    55561 actatcttat gaaaaaacga aacgaagtaa ggaagctata ccaaaggaat tcaaaaaact
    55621 tcatcgccgt ttcaacaaac tattaaaaga atttgattcg atccgagaaa tagcaaaaga
    55681 agatcattgg gattatcatc atcaaaaagc accggctgaa tctttagtaa aggcaggtta
    55741 tcaattaagc gaaaaaatta aagaatggct ggcagaattt cctgagcacc catcacaaga
    55801 acaattgctt ccttattatt ttgatttgct tcattttttg aaggtcagtg aattttatga
    55861 tgatcactac gaaacaacag tcgaaaaaac gtatcgcgat ctgatagtaa aagagttttg
    55921 tattgatcca agcttgtttc ttgagcagtc gttggataaa ggaaaaagca gtattctttt
    55981 ttctgctagt ttttcaccac tctcttatta tcaggaaaca ctggggggac aaaaaagtct
    56041 ggcttacaaa ctgccgagtc ctttcccaga aggaaaccag caagttttca tcgcaaatta
    56101 cctagagacg acttatcgaa aaagggaaca cagtctggca aaattagtag agacgattca
    56161 tgcttttgct gatggaaagg tcgggaatta tttcgttttc tttccttctt atcaatattt
    56221 agatcttgcg gtggaagcat tcagacagca atatcccgac agcaatgtgc taatccaaga
    56281 aacggacatg aatgaggaag aacgcgaacg atttttaaca aaatttcaaa caaatcccgc
    56341 tgaaacattg attggatttt gcgtacttgg cgggattttt tcagaaggaa tcgatttgag
    56401 aggggaccga ttgatcggca gtatgatcgt aggtgtaggt ttgccgcaga tgaatcatga
    56461 gcaggaactg atcaaatcat attatgatga gaaggaacac cttggatttg cttacgctta
    56521 tcagcttcct ggaatgaaca aagtcttgca agctggtggt cgagtgattc gtggcatggc
    56581 ggatcaagga atcgttgtat tggcagatca acgattttct agtttttctt atcgacgttt
    56641 attcccacaa cattggcaaa cagcaagaga agtacgaagt gttggggaat tggaagaagg
    56701 aatacagcgt ttttggttat caaaagaagc agtaaatcaa aaaaacgagg aatgacagac
    56761 gtcagcctcg ttttttgatt aacgatagtt agtgaattgc aactcgattg gtaagtcaag
    56821 tttacgtaat aaagcaatca cagcttgtag atcatcacgg ttcttacctg tcactctaat
    56881 ctgatcttct tggatttgtg cttttacttt cagtttttca tttttgatag ctgtagtgat
    56941 tttcttcgcg ttttcacgat cgattccgtt gaccaaatcg gcagtttgtc gagctttgcc
    57001 acctagagcg tgttctgatt cggaaaaatg gatatttttg attggcacat ttcttttgat
    57061 cagcttacca aataatacat ctttgatctg ttcgattttg aaatcatcat cgctcaatac
    57121 aactagattg ctgttctcta atttgatttc tactgtagaa tccttgaagt caaagcgatt
    57181 ggacaattct tttgttgttt gctgaatcgc attttttact tcttctgtat tgacttctga
    57241 tacaatgtcg aagctagcat cttttgccat aggaaccctc ctgatataca tataaatatt
    57301 taccatacat ctatcttatc ataggattag ggcagattct tacctgtttg ctaaaattct
    57361 tctcatttga gttcgaagta ttttatttta atagataaac tgttattctt acatttggaa
    57421 taccaaagta ggggagaggt gcttgtgagt aaaattatga ctgcgtatca taaaaatgaa
    57481 agattaaaga aaatagtggt tattttgata accggattga ccgctgcggt cgctttaaat
    57541 tattttttga ttccagccaa tgtcttttct gcagggatga atgggatcgc tcaaatcatt
    57601 tcctcgcttt tatatgaatg gtttcatatc aagatagaca caggaacatt tatttttctt
    57661 ttgaatatcc ctgtgtttgt attagggttt ctcaaagtag gaaaacgagc aacgatcctt
    57721 agttttgtga atgttgtatg tgtatcggtg atgacgatgc tgcttccagt cggacagatc
    57781 actgataata ttttgatgaa tgccctagta ggcggtgttt tattaggtct tggcgtagga
    57841 ttatctttaa aaatgggctt tacaactggt gggatggata tcatctctct tgtactttca
    57901 caaacaactg gaaaaacagt cggaaactac atgctgattc taaatggtat cattgtagtc
    57961 atcgcaggct tcttctttaa ctgggaaagt gccttgtata cgattatttc catttatgca
    58021 atgactcaag taattgatgc catccacacg agtcatcaaa aagtaacagc aatgatcgtt
    58081 accgtcaagc cagaagaggt aacagaagaa ctagcaaaac ggatggtacg tggaatgaca
    58141 ttactgccgt ctgtcggtgg ctattcaaaa aaacaaggga acatgatcat gatggtcatc
    58201 acccgctacg aaatgtatga tcttgatcag atcgttcatg aaatcgacga cgatgcattt
    58261 atcaatattt taccaactca ttcagtattt ggccggtttg caaatgaaaa cgaacaaaaa
    58321 atgtatcgaa caactggcgt attgccagaa ataaaaaatc gttcacaaaa aaaatgagga
    58381 atcaggttta ttttagaata aatttaatct attacatcta ataagaacat ttttataaat
    58441 ttaaacgaga atcttacttc taaattctca tttacaagca gctaaaaaaa gagtatgata
    58501 aataaaaatc gtgttaagga gtgtcgtacg atgagcgaag caaaccaaga acggtctgga
    58561 caagaaatag aactaatcaa agaacgtatt cttgctgcct tggaaatggt gattgatcct
    58621 gaattaggaa tcgacatcgt caatttaggc ttgatttatg atatagaatt caatcctgaa
    58681 aatggagaaa cagtcattaa gatgactcta acaactatgg gctgtccatt agcagatatt
    58741 ttaacagaat ctatccatga cgcattaaaa gaagtgccag aagtaacgaa agcagaagtc
    58801 aaactagtct ggtatccagc atggacgaca gataagatga gccgttatgc acggattgcg
    58861 ttggggatac gatagaaatc ttggctgact agctatgaga agcaaatagg aaaaacaaaa
    58921 aagcctttta tcctttctag aagaaaagag ataaaaggtt tctttgttta taaaattttg
    58981 tctgctatat tataagttaa ttaaataaat gtgttctatt gataaaggac attcaaatcc
    59041 ctttcctttt ggaatccttc ttttttacat cagtttgatt ttcgtatagt attctttctc
    59101 atcttcacga taaatcgcag ccccattttt ttgttgtgct ttaagactgc caagattttt
    59161 tttatttaca gacaaaagtg cctcatcaat gccgattttt tttgcctctt gtagtactaa
    59221 tgctaatcct tgtgtggcat atcctttttt acgataatta ggggagatgc cgtaaccaat
    59281 atggccagcg ccgtttctta atgtttcgtt cagtttatgg cgcaaattaa aaattcctac
    59341 atactcatgt tcatcacttt ctaaaataaa gacggttgcg ggaacgtaac tctcaggaag
    59401 atcgattcct aaagacgttt tttcgcattt ttttacataa tctaaaaatt cagcaaatgt
    59461 atagccataa gcattgttga taaatccact ctcatttggt ccaaagctag tttgtagctg
    59521 ataagctttc tttaaatctg cttcccatag tttgatcagt ttcatcgtat tcacctcttt
    59581 aagtaacctt attcatttgt tttctactat agcactattg ttgtaaatag ccatgcctat
    59641 ttttatttat tgtgtcatat cgtattaaga atcatcgaac aatacaaata tgaattacaa
    59701 taaaaaaatt atttgaagtt cgttcattcg ttacttatga tctctagaag aaagaaaacg
    59761 tgaattactt cagcgtctag aattccagaa ggatagtaat caacagaaga aataatcaat
    59821 atccaaatga aagaagatag ctgaatcgag aaaaagagga aaaaagaaat gaagaaaaaa
    59881 ataaaatagt tactaatccc agagtcgttc gtttaacatt gaaatcgatc atatggataa
    59941 aacagatttt ttcaccaacc ctcaatagat gatggagttg ctcggatgaa ttatttgtgt
    60001 agatggcata agggagggcg tacagaaatg aaaaagaaca aaaatatgtt cttgacaaaa
    60061 aacacatgta atcctaaact aaaagatgtg atgcggctat ggcggaattg gcagacgcgc
    60121 tagcttcagg cgctagtgag ggtgacctcg tggaagttcg agtcttctta gccgcataat
    60181 ttgttttttt ataaagagaa ggtacacctg gagcgatgag aaagaaaata acatattatc
    60241 taaaagaata taggaaaaat tttaaacaac ggaaccagat tcaaataaat caaaaaaaat
    60301 ttgtgtgaag gtgggacagt ttaaaaccta atagattgat tcaaatagta gatttataga
    60361 ttctgataca agaaatactg gcaaacgata gttaaggaat aaagactaaa cttaaactca
    60421 aaaagaggtc atgcgaaaga ataaatcgca tgacctctta ctatattttg aatgtgttta
    60481 aacgttcaaa taatgaatct tagtaccaac cgtttgcttc ccagaatgct ttcgccgctt
    60541 cccaagaacc ataacgtgaa gtaacatatt gttctgctac tctttcttgg tttgcagcag
    60601 agtagtcacc atttaagtaa gaagaatcta attggtaacg gccgatgtaa cgtccgttcg
    60661 ttgctgtgta agaaccgctt gattcttttt gtgcgatcca ttcttttgca gagcttgtac
    60721 ttgctggagt agcagcagtt tctgttacag caggagcagg ttgtgtttca gcaacaggcg
    60781 ctgcttgtgt ttccgtttga accgtttctt gtacaacagg ttgttcagct tgaacaggtt
    60841 cagcttggac tggtgcttct tgaacggctg cttgaggttc agcagctgct tgtgcagagc
    60901 cttctgttgg gattgttaat tgttgtcctg agta
//
LOCUS       DAZUKC010000007        52289 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000007 DAZUKC010000000
VERSION     DAZUKC010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.007"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            261..1304
                     /locus_tag="ACG80T_000398"
                     /pseudo
     CDS             261..1304
                     /locus_tag="ACG80T_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
     gene            complement(1509..2828)
                     /locus_tag="ACG80T_000399"
     CDS             complement(1509..2828)
                     /locus_tag="ACG80T_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HFS4840081.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            3085..3555
                     /locus_tag="ACG80T_000400"
     CDS             3085..3555
                     /locus_tag="ACG80T_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFS4840082.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            3777..4880
                     /locus_tag="ACG80T_000401"
     CDS             3777..4880
                     /locus_tag="ACG80T_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFS4840083.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            5101..6060
                     /locus_tag="ACG80T_000402"
     CDS             5101..6060
                     /locus_tag="ACG80T_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS4840084.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            6230..6910
                     /locus_tag="ACG80T_000403"
     CDS             6230..6910
                     /locus_tag="ACG80T_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFS4840085.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            7000..8034
                     /locus_tag="ACG80T_000404"
     CDS             7000..8034
                     /locus_tag="ACG80T_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFS4840086.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            8048..8383
                     /locus_tag="ACG80T_000405"
     CDS             8048..8383
                     /locus_tag="ACG80T_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840087.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            8367..10847
                     /locus_tag="ACG80T_000406"
     CDS             8367..10847
                     /locus_tag="ACG80T_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840088.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            10903..12462
                     /locus_tag="ACG80T_000407"
     CDS             10903..12462
                     /locus_tag="ACG80T_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4840089.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            12459..13823
                     /locus_tag="ACG80T_000408"
     CDS             12459..13823
                     /locus_tag="ACG80T_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFS4840090.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            13994..15064
                     /locus_tag="ACG80T_000409"
     CDS             13994..15064
                     /locus_tag="ACG80T_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4840091.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            15079..15747
                     /locus_tag="ACG80T_000410"
     CDS             15079..15747
                     /locus_tag="ACG80T_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4840092.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            15767..16345
                     /locus_tag="ACG80T_000411"
     CDS             15767..16345
                     /locus_tag="ACG80T_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4840093.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            16746..17657
                     /locus_tag="ACG80T_000412"
     CDS             16746..17657
                     /locus_tag="ACG80T_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFS4840094.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(18035..18952)
                     /gene="rnhC"
                     /locus_tag="ACG80T_000413"
     CDS             complement(18035..18952)
                     /gene="rnhC"
                     /locus_tag="ACG80T_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFS4840095.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            19154..19591
                     /gene="zapA"
                     /locus_tag="ACG80T_000414"
     CDS             19154..19591
                     /gene="zapA"
                     /locus_tag="ACG80T_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFS4840096.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            19649..20197
                     /locus_tag="ACG80T_000415"
     CDS             19649..20197
                     /locus_tag="ACG80T_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFS4840097.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            20312..22672
                     /locus_tag="ACG80T_000416"
     CDS             20312..22672
                     /locus_tag="ACG80T_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFS4840098.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            22759..23073
                     /gene="trxA"
                     /locus_tag="ACG80T_000417"
     CDS             22759..23073
                     /gene="trxA"
                     /locus_tag="ACG80T_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFS4840099.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            23255..25033
                     /gene="uvrC"
                     /locus_tag="ACG80T_000418"
     CDS             23255..25033
                     /gene="uvrC"
                     /locus_tag="ACG80T_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFS4840100.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            25178..26077
                     /locus_tag="ACG80T_000419"
     CDS             25178..26077
                     /locus_tag="ACG80T_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4840101.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            26070..27332
                     /locus_tag="ACG80T_000420"
     CDS             26070..27332
                     /locus_tag="ACG80T_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4840102.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            27493..29391
                     /locus_tag="ACG80T_000421"
     CDS             27493..29391
                     /locus_tag="ACG80T_000421"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFS4840103.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(29721..30440)
                     /locus_tag="ACG80T_000422"
     CDS             complement(29721..30440)
                     /locus_tag="ACG80T_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFS4840104.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    30630..30677
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            30777..31109
                     /locus_tag="ACG80T_000423"
     CDS             30777..31109
                     /locus_tag="ACG80T_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840105.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            31096..33099
                     /locus_tag="ACG80T_000424"
     CDS             31096..33099
                     /locus_tag="ACG80T_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFS4840106.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            33118..33588
                     /locus_tag="ACG80T_000425"
     CDS             33118..33588
                     /locus_tag="ACG80T_000425"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFS4840107.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            33609..34193
                     /locus_tag="ACG80T_000426"
     CDS             33609..34193
                     /locus_tag="ACG80T_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFS4840108.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            34208..35194
                     /locus_tag="ACG80T_000427"
     CDS             34208..35194
                     /locus_tag="ACG80T_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFS4840109.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            35184..35495
                     /locus_tag="ACG80T_000428"
     CDS             35184..35495
                     /locus_tag="ACG80T_000428"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFS4840110.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            35517..37298
                     /locus_tag="ACG80T_000429"
     CDS             35517..37298
                     /locus_tag="ACG80T_000429"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFS4840111.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            37291..38667
                     /locus_tag="ACG80T_000430"
     CDS             37291..38667
                     /locus_tag="ACG80T_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFS4840112.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            38674..39309
                     /locus_tag="ACG80T_000431"
     CDS             38674..39309
                     /locus_tag="ACG80T_000431"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFS4840113.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            39339..40694
                     /locus_tag="ACG80T_000432"
     CDS             39339..40694
                     /locus_tag="ACG80T_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFS4840114.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(40886..42280)
                     /locus_tag="ACG80T_000433"
     CDS             complement(40886..42280)
                     /locus_tag="ACG80T_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFS4840115.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            42372..43343
                     /locus_tag="ACG80T_000434"
     CDS             42372..43343
                     /locus_tag="ACG80T_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFS4840116.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            43340..44122
                     /locus_tag="ACG80T_000435"
     CDS             43340..44122
                     /locus_tag="ACG80T_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFS4840117.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(44291..44662)
                     /locus_tag="ACG80T_000436"
     CDS             complement(44291..44662)
                     /locus_tag="ACG80T_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4840118.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            44796..45239
                     /locus_tag="ACG80T_000437"
     CDS             44796..45239
                     /locus_tag="ACG80T_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFS4840119.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(45282..46091)
                     /gene="yidA"
                     /locus_tag="ACG80T_000438"
     CDS             complement(45282..46091)
                     /gene="yidA"
                     /locus_tag="ACG80T_000438"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFS4840120.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(46110..47477)
                     /locus_tag="ACG80T_000439"
     CDS             complement(46110..47477)
                     /locus_tag="ACG80T_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS4840121.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(47461..48291)
                     /locus_tag="ACG80T_000440"
     CDS             complement(47461..48291)
                     /locus_tag="ACG80T_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFS4840122.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            48454..48891
                     /locus_tag="ACG80T_000441"
     CDS             48454..48891
                     /locus_tag="ACG80T_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFS4840123.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            48967..49587
                     /gene="rpoE"
                     /locus_tag="ACG80T_000442"
     CDS             48967..49587
                     /gene="rpoE"
                     /locus_tag="ACG80T_000442"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFS4840124.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            49776..50282
                     /locus_tag="ACG80T_000443"
     CDS             49776..50282
                     /locus_tag="ACG80T_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFS4840125.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            50299..51198
                     /locus_tag="ACG80T_000444"
     CDS             50299..51198
                     /locus_tag="ACG80T_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFS4840126.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            51285..52169
                     /locus_tag="ACG80T_000445"
     CDS             51285..52169
                     /locus_tag="ACG80T_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFS4840127.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
BASE COUNT        17309 a         8991 c        10992 g        14997 t
ORIGIN      
        1 taaaacagca tagttctcaa attgtttaaa atgattgtca agttaaaaaa atctgggatc
       61 atataaaata gtatagttct caaatatgat tagaaaccct attaaagagc aatattaagc
      121 caacttactt aaatcgttta attaagaaca gtaatctctt ttagtcagac ttttagtctg
      181 acttttttgt atttccaaaa tttattcttt acaaaaacga acatatgttt gtataattgc
      241 ttgtgaatgg gggaataaaa atgtttgatt acagtagaga accggtgaat gactattttt
      301 taatcgacat gaagtctttc tatgctagcg tggaatgtat tgaacgaaat ttagacccaa
      361 tgactacacc gttaattgtc atgagtagat cggacaacac tggttcaggt ctgactctag
      421 cttcttcacc tactgcaaag aaaaaatatc atattactaa tgttagtaga gctactgatt
      481 taccttatcc attaccaaaa gaacttcata ttgttccacc acgaatgaac ttatatataa
      541 aaaaaaatat ggaagttaat cagattttta gaaagtatgt cgcagatgaa gatttattga
      601 tttattcgat tgatgaatca attttaaaag tgacaaaatc cttaaattta tttactagtt
      661 caggaacaag aagcgagaga agaagagaat tagctaaaat aatcaaagat gagattcaga
      721 aagaaacagg tctaatagca acagtcggaa taggagataa cccattatta gctaaactcg
      781 cactggacaa tgaagcgaag agtagtaaag aatttatagc tgagtggtct tatgagactg
      841 tacctgaaaa aatatggaaa ataaacgaga tgactgactt ttggggcatt ggatcacgca
      901 tgaaaaaaag attgaatcat atgggaatta tgagtataga agatttagca aattggaatc
      961 cctacacaat taaaagtcag ttaggtgtaa tcggacttca gttatatttc cataccaatg
     1021 gaattgatcg tacggatatt gctattccac cagaaccaac caaagaaaag tcttatggga
     1081 attcacaagt tctaccaaaa gattatacga ggagagaaga agtagaacta gtagtcaagg
     1141 agatggcaga gcaagtagcc attcgaatta gacaacacaa ttgcaaaact ggttgtgtac
     1201 gcttagcaat tggtacgtca attttggaag aaaaatcagg tttctcgcac cagatgaaaa
     1261 taccaattac agataatacg aaagaattac aaaattattg tctttcctga ataattcata
     1321 gctttaattc tttggcacat tttattgaaa tatgtatcac taatatccct atcaactgat
     1381 tttgactaaa aagttacttc aaacattcac tgttcaaaaa tattgaggaa taaattttgg
     1441 gtgtaaggca ttttgagcat acttctccct ggataacttc ccagtaaaaa aatcgttaga
     1501 ttgttggatt agatccattg actggctcgc ctcaggcgag caaagaccct gtagaattca
     1561 ttctgataca catgataact agataatttg agatagactc gtctacccgt ttggactaat
     1621 ttcccagcaa ctttcagaaa cttcaatcga atagaatgaa tggtcattcc cttttggact
     1681 tcttcaaagc caatcgttct taagaagttg actaagttgt aagctatcaa gctgagcatc
     1741 attcggacat gattctccaa aaagcgagga ctgtctgttt tgtcaaagta aaagccagct
     1801 ttcgcttctt taatgaagtt ctccattgtg ccacgtttgg catagataga aaagatggta
     1861 tcaggagaaa cattttctga tagattcgtc acgataaatt catgtcgaaa gagtagttcg
     1921 cccgcttcac gtgttgagcg tatacacacg cgacgacttt gtgaccagga ttgtgcttga
     1981 taagtggtcg agaagtactg aacttctcgt tcttcccact tttgattatc gccataaagt
     2041 actgatttct cagctatttg acctagtcta cgattattct tcagtcgaat aacataatga
     2101 ctcttttttg attcacacga atcatacaca ccaggtgtag cgaacccgct gtctccacga
     2161 accaagatgt cagtgtttgg tagagagtga ttatagtgct ctaataaagg tgtgagaaac
     2221 tcctttacgc cttttgatgt atattgattt cctgaacgta gttcagcttt taagaagtca
     2281 ccagtcaatc cgtcaaaagc taccaatgga tggtaaccat aggtttggta gtgtgtatta
     2341 taattcgttt gttcttggtg accaaatgta tctgaatggg tcgaatctaa atcaatgatt
     2401 aattccgtat cattacggat gaggcgtgct ttatcgataa gttcttggtt caaagcttga
     2461 agttcatgga tattctcctc agatagtcga tctaaaaatc gggaaagtga agattgagaa
     2521 gcgagttctt tcctatctaa aacagcttta aacacaggat cttgtctcag gagattagct
     2581 gcagaatcag ctgagtaacc agcaattaat tgcataatga actgctcaag tatggataag
     2641 ttgtcatgag taaaatacgc tcgttcgtct tcaatgtgaa tgtgttgctt agccagttca
     2701 gaaaaaccaa agatgttcat cagctctttc actaggacga gacccgaatc actcgataat
     2761 tgaccacctg tatgagaaat agtgatattt gaattgaatt ttacttggtt tttgtgtaag
     2821 ctagtcatga aaagaactcc tttcttttgg ttggtttagt cactttaacc atagcagaaa
     2881 ggggttcttt tttcatcact taacgggtga agatgaaaaa gtaattgtaa gctgccgtga
     2941 ggcagtttca catggtttta attaaaagta tgaattattc aggtagaatc tctgaagaat
     3001 aaatcacgtg atcgtgacga taaaatacac ttacgtcagg tgataaaaaa tacataagta
     3061 gaaaactaag aggaggaaca gaaaatgaaa gaagttcaat tgatcagaaa aagcgaacta
     3121 tcagaaggtg gttgcaatgc atgcggcgta gtagaagcaa caagctatac attaaagctt
     3181 ggtgccaaca aagcgataat ctctgagttg acagtaggcg gtcttgtgga cagtctggcg
     3241 cttgctgaag gttttattgg agaagatatt tatgagatgt tttcagaaat ccgtcagtta
     3301 aaaaaagggg aaaactgcat agaggttcat catgagagcc cgaatgtgag attcaaacgt
     3361 ggtgataatg agatgatctt caataatcat gtgtccgatc acaccgagct atatggaatc
     3421 gtgaatcaaa tactaactga gctttttgga ttgggacctt atgcgttcaa ggaagaaaac
     3481 ggtaatccaa aattaaacga agaatggcaa gaaacgattg aaatacaaag aaacaacccg
     3541 cacttatttc aataggcatc cacagtattt cgtaaaaggg actttgacac gacttttgtc
     3601 aaaatcccat cgttttttat ctatttgtgt tctaaacatt tctttcagag tagtaacaac
     3661 tggaatgact gaaatgtaaa cagattattt gcctgtatca atacggaaga gtaatataaa
     3721 gtgtgtaaac gctatcatat tattcattca attttttata gagggaaggc attgaaatgg
     3781 aaaaaacgta tcaagcaggt acaaatgaag gaatagtgga ttttatcaat atggaagatt
     3841 tagaaatagc cgcttcacaa gtgattccag ctggtggtta tggttacatc agcagtggtg
     3901 ctggcgactt atttacttat caagaaaatg aacgggcatt caatcatcag ctgattattc
     3961 ctcatgtatt aagagatgta gaattacccg atacaactac acattttgac aaagaaacgc
     4021 tgactgcacc aatcatcatg gcaccagttg ctgctcatgg cttagctcat gtgaaggcgg
     4081 agaaagcttc tgcaaaaggt gtagcagatt ttgggacgat ctatacagca agttcttacg
     4141 cttcttgtac attagaagag ataagagaag caggcgggga gaaagcaccg caatggtttc
     4201 aattttatat gagcaaagat gatgggatca atttggatat tcttgaagtg gccaagcgta
     4261 atggagcaaa agcaatcgta ttgacagcgg atgcaacagt tggcggaaac cgtgaaacag
     4321 accgcagaaa tgggtttacc ttcccactgc caatgccgat cgttcaggct tatcaatcag
     4381 gagtaggcca aacgatggat gctgtgtaca aatcttccaa acagaagctt agtccaaaag
     4441 atgtagaatt tattgctgct cattctgatt tgccagtcta tgtgaaaggt gtccagtcag
     4501 aagaagatgt ctatcgttca ttagaatctg gtgctggcgg aatctgggta tctaatcatg
     4561 gtggccgtca actagatggt ggtccagcag cgtttgattc gttgcaatat gtggcagaag
     4621 cagtagataa acgtgtacca atcgtgtttg acagcggtgt acgacgcgga cagcatgttt
     4681 tcaaagcaat cgcatcaggt gcagatcttg ttgctattgg cagacccgtt atttatggat
     4741 tgtctttagg aggaagtaca ggagtccatc aagtgtttga tttctttaaa acagaattgg
     4801 aaatggtcat gcaattagct ggaacacaaa cagtagaaga tatcaaaaaa ataaaattgc
     4861 gtgagaatcg ttttatttaa gaaacaaatg acgaaaaaaa taggagtgcg gcagatgatg
     4921 ccgtactcct atttttatag ttcaaggatg tcactaatag aataatttga aacgaaaaaa
     4981 taaaattgaa atattttttc atacttcgcc gattgtaaaa ttaagctaga caactaaaaa
     5041 aagtcatttg tgctacactc aaaatagttc cgacgaaaga attataagga gtgagtacaa
     5101 atgacctata accatcttac accaactgaa ctcgtaatga tagaagctta tttcaatcaa
     5161 tcacaacctg tttcaaaagt agccaatctg ttacaacgtt ctcgacaaac tatttataag
     5221 gtctatcgtt ttcttaaatc tggtggaagc gcaatgagct actataaaca atataagaaa
     5281 aacaaacgta actgcggaag acatccgatt gttttacctg atgagcaaca ggaatacatt
     5341 caaaaaaagg ttgttcaagg gtgggctcct gatgtcctta ttggtcgagc agagtttcct
     5401 attgcttgtt cggtacgcac actgtatcga atgtttaaag ataaaacctt tgacactcac
     5461 gatttaccaa tgaagggaaa aagaaaacca aacggtcata aagaaaaaag aggaaaacaa
     5521 gggtttcgcc ggtcaatccg agacagaaag acagactata cccagtttga tcatgaattt
     5581 ggtcacctgg aaggtgacac tatcgtgggt ggaaaacata aaagcgcggt catcacatta
     5641 gttgaacggc tatctaaagt catcattacc ttgaaacccg aaggcagaca ggctaaagac
     5701 attgaaaata ggctgaatca atggtttcgt tgtgtcccaa aaaacttatt taaatccatc
     5761 acatttgatt gtggaaaaga attctcaaat tggaaaaaga ttagtaatac caacgatatt
     5821 tcaatttatt ttgcggatcc aggaacacct tctcaacgag gattgaatga acattcaaat
     5881 ggcttattac gtaaagatgg tttgtataaa gctatggatt ttaatggtgt atctgaaacc
     5941 tttatccaat cggtggcatc gaaacgaaat catattccta gaaaatcatt acactatcgg
     6001 acaccaatgg aagtattttt gagttacgtc acaaaagatg atttgtctaa cttaatttga
     6061 caaacgaaac ttaataaaaa aggaagaata ttcatgtaaa cgattttatt gtttttttct
     6121 cttttataca aaaagggtca taatttcaag taagcggatg aattcatgag gattttttgc
     6181 atactgtctc tactatactt aggtcactaa gaaaaaggag tgatttatta tgaaaaagaa
     6241 catgattggg acattattac tttcaacact tcttctaggc ggcttggcaa caccagcttt
     6301 tgctgagggt caagcgactt caaaaggaga tattaccttt actgaaccaa caaataccgt
     6361 ggagcctctt aaccctactg atccttcaaa accagtagag ccagcagatc cggaaaatcc
     6421 agcaactggc cagacaggtc ctttaacact ggatgtagtt cccgaattgc catttggtac
     6481 acacgagatt gaaagcggaa caaaaacgta tcaagtagac gcttcaaaaa acgatacacc
     6541 ttatctccaa gtatcagatc gtcgaggtgt aggtgcagat ggccaagcac aagggtggaa
     6601 cgtgacagtt tcggtttcag attttgtaaa cggttcacaa gttttgcaag gcgctgaatt
     6661 agattttgga acatccactg ttaaaagtac gtcagataac gaatccactg gaccaactag
     6721 tcaaactgta actgggttga gtaaagcaag tgcagcgaca ccaatattta cagcagcaaa
     6781 agatcaaggt ctgggaacat ggctttctgt ttatgatcca gcaaatatca cactgaaagt
     6841 gccgaaagca gcagctggaa cattcacggc tgatttgact tggaacctcg tggctggccc
     6901 agtagcatag agtatgaaat aaattcaaca aactgcggtg aaaatactat aggtgaagga
     6961 ttttttaccg cagttgttgt tgaaaggatg tggtggacaa tgaatcaaag taaacgactg
     7021 tttgtttcgt ttttttctat tttaagtcta tttttcattt ttccgtcaat cagccaagca
     7081 aaggattcag caggagactt tggaatcaaa ccggtatttc cagagaacca aatagataaa
     7141 gcaatcggtt attttgattt attagtggca ccggaacaaa atcagacact agaagtaatt
     7201 atcagcaatt cttcagatga agaacgaaca tttgaggtct ctgtcaatcc ggcggtaacg
     7261 agtgatggtg gaacgatcga ttatagtcaa aaaaacccaa cgttagatga gacattgccg
     7321 tttgatgtcc gtgacgtttt gctcattgcg aaaaaggaaa tcaatgtatc agcacatgca
     7381 gaaacaactg tacctataga agtgaaaatc ccagcaaaaa gctttaaggg acgtgtatta
     7441 gcgggtattc atgtttcacc taaagaagaa gctgaaacag aaaatgctac agaaggagca
     7501 caaatcaaaa atcgtattgc ttataatctc gcagtcgttt tacaagaaag tcaagagacg
     7561 atagaaccgg atctgaaact tttatcagga gatttggacg aggtcaacgc aaagccaact
     7621 gttcagcttc gtttccaaaa tcctcagcct aggattatca gcaatttgat atttacgagc
     7681 aagatctttt atgaaaatca gctttatatt gaaaatacgt ctaatgcatt tttagttgca
     7741 ccaaattcga attttcactt gaatcttgat ttagctggtg ataaagcaaa agccggcgat
     7801 taccgcgcag aaatcatagc aaaatcaggc gacagtaatg aatggcgatt tacacaaaac
     7861 tttacgatca aaaaagaaaa agcacaaaaa gtaaatgaaa attccgtttt tgcagtacaa
     7921 gaacaatctt tcccatggct ttatgcagga ttggttgctg cagttctagt gttatggttg
     7981 attttgctgt ttttgtgggt ccacaagaaa aagaaaaaag gagaaacaac atgatgaggt
     8041 gatttcgttg aaaacatcga tcttattggg agggattatt gcaagtttgc tgttcttcag
     8101 ctggcaagag ccagtccaag cagataccaa aagttcaggt acgattacat tttatgatct
     8161 aagtccaatt ccgcatccaa gtggttcagt caatggcgca tcaaaacagc agacacagct
     8221 accgatttat aaagaatcta atcaacatgg cgtattcccg gagacgaatg aacaaaagat
     8281 ccgttttctt tatttgatca ttggtataga attatggctt cttgcgtggt tgttgttatg
     8341 gagaagaaag gaaaaggagg atgaacatgt tgctacaaaa taaattttca ttttttcgct
     8401 ccttcttttt tacttcgctt tttgttttaa gtatatttat cttttcacct tcggcagaag
     8461 ctgttggagc aacgcctcct gccatttctt atgatccata cacgtatgaa aatatcgtac
     8521 ctccaaatgg tggacgtagt gcagtcaatg gcgggaagct tgtccaacta actggggcta
     8581 accaattgcc taaattgact gctacttatt tgaacgatgg ttcaaatgat ccttggaaag
     8641 gtggcacggt ccgttttcat tgggaaagcc atgtgaaact aacaaatgta cttgatttgg
     8701 tgaatatcac tgcacaagga tttgcatttg tggcgatcat caatttgccg gaacatgtat
     8761 caagcagtga tgtattaaaa gcaattgact ggaacaacgc ctacttgtat attgccggtg
     8821 cccccatcaa ggttactagc ggaagtttat tatcagcagg aagccatacc ttgcgttttg
     8881 ggatggggtc gtgggataca aatactctat tgacagatat tattgctatt attacgcgag
     8941 gcgaattcaa cgtggacgat atcccgcttc aatttgatgt tacagctaat gtcgcgaaga
     9001 tgacagaaaa cggagataaa tatgatacga gtccaaacaa aattttgacc aagggtaaat
     9061 tacaaccttc tatgtctaaa aaggccgttt tttctgttga tttttatgat tcgaataata
     9121 ttatccaaga tcgtatgttc aattcgattt tagctccagc tgcaggtaga ttttatccta
     9181 aattaactgg cggaaacttg aatccaggag catatgcatt ttctgcaagt gcagagataa
     9241 atacgtggaa tagttacatc tcaccaagtg attcaacagg aaaatataat acatttgctg
     9301 ataccacaga atacgtggat ggaaataacc agaaccttcc aggctctacc aatagtcgga
     9361 cattgacgat ggggctgact gaaggacgtt ttgatcagtt gccatttagt cgctttgatc
     9421 gcgtagtcaa ctattttaca ggaaaaaacg ttacacaagg agcaatactg actcatactc
     9481 cagcaagcac aagtggattg aatcagacag ccaatatcgt gtacagtggg aaagatgcgc
     9541 aaggcggatt gctttctcct gttgctttaa aggtattgga gcagtacaag ctagacggaa
     9601 cggttttacc aacaaactta acagccgata gtcagtgggg aaagctcctt tcttctgcat
     9661 cgtaccaagt cacggccaat tggtcagcaa ctgaactaaa aaagggttcg atccattaca
     9721 ggctttattc gaaaggaacc caaaaattag cgacaggatg gacagatcaa cttttccaaa
     9781 cgatccaatc aaataacggg ggtgtaaata cagctacttc tacactacct gcattggctt
     9841 cagggcaata ttattttgat tatcgcttgg tggacgatgt acttactaac atgtatccgt
     9901 cttttgcgta caaatggcag tctgaacaag ctggaatcac cactcttcct caaataacag
     9961 tggctaattt tccatctgtg agtgcagata gtcagctaaa aaaccttagc agacaacctg
    10021 agacaggaga tcccctgtct gctctatcag gagatacgat tcaagaaaat gtgacattca
    10081 cgttaacaaa atctggggat gcgataaccg acaaaaaaat cacgatctca cttcctgaaa
    10141 atactactta tatcaatggt tccttaaaat taaatggtac aactatcgca gataccggca
    10201 ttcaacaagg agtttctgtc ccagcagatc tattgagcaa aataggagat accattcatc
    10261 ttacttacaa ctatcaatta aatactgtag atacttcttt acaatccgtc agcatactga
    10321 caaaggcagc tgtactttcc agcaatatta cattagcaga tggtgctaag cttcctaacc
    10381 cagttgtcca aacatctgct aaaacaattc ttgtccccaa acaagaatta acattagtta
    10441 atgtaccaga tgattttaca tttggtaacg atctccccaa accgttgaag acaagttatt
    10501 atgaatcaaa aggcgatttt tcttttgatg tgcgggatac tcggcttcca agcacgtctc
    10561 cttggcaact gacaggtaca ttgacttctt tatttaagaa tgatcaaggt caagaattaa
    10621 gcggaacaaa attatacttc aatcattctg gatcaaagca attgattcag caaggtcaaa
    10681 acacgttgat ttatgagagt gatggaacag ctaaaggaga ggttctcgtt gactttcccg
    10741 ataccgatgg tttactctta gaggtaaata gcagtacgaa tgctcaacct ggcgcaacgt
    10801 atcaaggcat ggtcacatgg gaactgacag ctgggccaac cagctaagtg attagaattt
    10861 gtatggatct gtgataactg tcacagatcc tctttttcaa ggatgagaag gaaacttctg
    10921 ctgttaaaat cagaaatgct tgaaggagtg gacttggaac ttttcagttt gcttgaaaaa
    10981 aatgaacaac ggcaaatcaa acttttccaa cttttattag aggaaaaagg agaggtttcc
    11041 gttaataacg tacttacccg cttatcagtt gatcgggcta ccttgaaaga agatctgttt
    11101 tatttacaag ctaacttgga aaatatcaaa caattggaaa ttgttatcca agaagggaat
    11161 attttattaa tcagacatgg aaatatagca gcagtcgatg tttattatca ttgttacttg
    11221 cgctattcta caaaatatca gatacttctt tatctgttgg aaaatggctc atttgatcgg
    11281 cagaaacttg tcatggaact taatatcagt tcggctactt tgtctcgcag agtgaaggaa
    11341 ttaaatgatt ctttaaatga gtttcatctg aaaataaaaa gcgggcggct gaccggtcca
    11401 gaaagtcaaa ttcgttattt ttatttccaa ttattttggt ttggacgtcc ctatcttgaa
    11461 aacaaaaaag aatacagtga tttatcagga aacacttttt tagatctgct gcaaaaagag
    11521 tttagttttg ctattacaga agaaggacga ttgaaatttg aaatatggct ttccattatg
    11581 aagaagcgaa tgcgctgtac gaaaaaatcc aaaccagatt atagcaatct tcctgttgga
    11641 ttgtttcaaa cgacacaatt tttattggac ctccatacat ttttgacccg ttacttcttt
    11701 caatatgcct ttccttggaa tcaaaatgaa acgttggttt tttatctttt catgatcagt
    11761 aactttacat tagatacaca taactcgcat gtccaggagt ttttgataag cgaaaaatac
    11821 aaagacgacg tagtagctca attgaatcga acgtttaaac aatcgataga agaatatttc
    11881 tctttttatg tttttccaga agagatttcg caaaagattg cgttgacagt gatgcaaaat
    11941 cacgctcatt ttgcctattt tagaggatgg atgaatattt ttggccaaga aggattaaaa
    12001 cagcggttaa ctgaagtaga agataaacaa ttcctggttt tatgtgaaag attggtcaga
    12061 caaacatttg ctattgttga ttttactgaa gtagacgagg aaaatgcccg ctttgaatta
    12121 tttgctcgct atgcatctat tttacggacg ctgatcaagc agttagaatt acgtctgcaa
    12181 attgcttatg atcttcctta tgaaaggcta atggcggata tcgtgaaaga aatcatccag
    12241 gaacaattgc tgaatacgat caaatatgat ttagtcgaat acgagaaaga aaagcagtat
    12301 gatatcattt tgactagcca attgaaagag tacccgtcac agaagtccgc taaagttttt
    12361 gtattcacta gcaacgaata taattatgat ttcccctatt tgaaccaatt tttaaaagaa
    12421 tgttatttag aaaaattgaa tttaaggatg aataaaacat gaaaatcgtg atcattggtg
    12481 cttcccatga aggcatcaca gctgccctaa atctaagaaa acttcaaccg aaagcagaag
    12541 ttttactgat cgatgaagat cataaagatg gactaggcta tgtttcaaat ggaatcaacc
    12601 tttatttaaa aggaaagata cgtagtttag ctgaagttgc ccataacatg cggactcttc
    12661 aagaaagtgg ggcaaaactg atcaccgaat ggagagtaac tgaacttgat ccagacaaac
    12721 atcagctcat cctcgcatcc aaagaaggaa aaaacgagac cataacctat gataaattaa
    12781 tcgtagcgac aggttcctct cctgtcacct tgtataaaca aattgaagct gaaaatgtct
    12841 acacctataa aaatctagtg caatctaagc aagtacttgc cgctttaaaa gaagcaaaag
    12901 aagtagtgat cttcggtgct ggttacattg gattggaatt agcagatgct ttgagaaata
    12961 aaggatacat tattcatcta gttgattata tgccgaatgt tttatcaaga tatttcgata
    13021 aggatatgat caacagtttt caaaatcagc tgcaaactaa gcaaataaat ttttatccaa
    13081 atgaattctt gatcgattgg aaaaaatcag aagagaaagt agtatccgta caacttctgt
    13141 ctcaaacaat aaaggcagat atggtcattt tttctgccca aactcgtcct aatacgacat
    13201 tgttaaaaga aaaagtggct ttgtatgagg atgagactgt gatagtcaat gaatatctgc
    13261 agacaagtga tccggacatc tatgcgattg gagatatcgt cccagtcagt tttgacaaaa
    13321 ataaacggca tctcttttta cctttagtta ctcgagctgt acatatggca agagcagtag
    13381 cgctaacttt gagtggtcag ccaaccgctt acgacctcag acaaaagata acagcagcag
    13441 tcattataga ctacttttta ggaactgtag ggctgacaga agatgaagcg ccattcttgg
    13501 aacaaaatac cggttcgtgt tcaggagaat ttgatctatt tccacaatac gacgaagaaa
    13561 acaaaacagt aaatgcaaag ctgatctacc atccggatac attagaaata attggcggac
    13621 agctgataag ccaagaattc ttattatcag atttgaacct gcttgcggat atcgtgaaac
    13681 acaaaactac gattccacag ctagcagttg aggattttgg atttctgtct gaatacacct
    13741 ctcgttttca ctacctcaat gagttagctt ttaaagtact agctaagaga gcaaatagca
    13801 atagagtaga taaaagactg tgagaaatat cacggtcttt tattgtgtgg ggatataata
    13861 aatattaatc aatcgagaat aaaaaattca tataatgaaa tagcggaaga attttttgag
    13921 cataatattc gccaaaaatt tatcaagtga taaaattgtt gtataaaatg attatttcag
    13981 gaggaggagt taaatgtttt tagcattaaa tgaaattatg cattcaaagt tacgttatgc
    14041 gttagttgca ggggtgatgt ttttgatcgc ctacctggta ttttttttga ctgggttagc
    14101 ttatggctta gctcaagata atcgaacagc agtcgacaag tgggaggcag attcgattgt
    14161 gctttcgaaa gatgcaaatt caaatctagg gatgtccatg attactaaga agatagcaga
    14221 agaagtcgaa ggtgggaaag tggcgtatct tgcccaaaca cctggagtcg tgactagtaa
    14281 agacagtaca gaagaaggaa aaatcaatgt tagcttcttc ggtatcgata aaaatcaatt
    14341 catcatgccc aatcttgttg aaggaaaagc ttttgataac gatgatgagg cagtaggcga
    14401 tattagtttg aaagaagaat atggtctagc cataggagat acagttaagt tatctggtag
    14461 tgacaagacg tttaaactaa ctggctttac ggatcatgcc aaattcaatg tctcaccagt
    14521 tctctataca accatcaatg cttaccaaga aatccgcttc gaaaaagaag atacaagtga
    14581 aaatgcacgg attaacgcaa tagttgtcag aggtaaaatc agtaatttac ctgaagattt
    14641 agaacagatc aaaatttcta aattcatcaa tgaattacct ggatataatg cgcaagtatt
    14701 gacttttgga tttatgatcg gatttttgat cgttattgcg gctattgtga tcgggatttt
    14761 catttatgta ctgacgatgc aaaagattaa tatctttggt gtaatgaaag cacaagggat
    14821 cacaggagga tttattgcgc gttctgtagt ggcacaaaca ttcattttat catttgttgg
    14881 tattcttctt ggtctattag gaacagtggg aacatccctt gttctacctg atgcggttcc
    14941 attccaaagc aattggctgt tttttggcgt cattagcttg ttgatgttag tagtagctgt
    15001 gttaggcgca ctattttctg ttcggacaat cgtaaaaatc gatccgttga aggcaattgg
    15061 ataagaagga ggaagaacat gaaagcaata gaatttaaag aagtagaaaa aagtttccaa
    15121 gatggcgacc aagtagttaa agcattgaaa gaaacaacca tgagtatcga taaaggggaa
    15181 tttgttgcaa tcatcggtcc ttctggttca ggtaaaagta cttttttgac tttggcaggc
    15241 ggattgcaga cgcctagcag tggctctgta tttatcaatg atcaagaatt cagtaatcaa
    15301 aaagaaagcg aacgcgttaa actacgtttt aaagaaattg gctttatttt gcaagcttcc
    15361 aatctggttc cttttctgac agtaaaaaag cagttattat tagtagataa gattaaacat
    15421 gaacatcgtc aaaaagaagc aaacgaatta tttgagcagc ttggcgtgga taagttgatc
    15481 aacaagtatc cagaagaact atctggtgga gaacggcaaa gggtcgcaat cgctcgtgca
    15541 ttgtacaatg atccgtccat tattctagca gatgaaccaa ctgctagttt ggattcagaa
    15601 aaggcacgag aagtagtcga tatcttggca aaagaatcaa aagacaaaaa taaagcaacg
    15661 atcatggtta ctcacgatac acggctgatt gacaaatgcg atagaatatt tatgatggaa
    15721 gatggtgtat tgaaagaaaa agaataacca aaaagcaggt ggaaaaatgg tccatcgata
    15781 caacaaagat acacagcaaa aaattttgaa agaagcgact gacttgttta tgtccaaggg
    15841 atatctcggc acttcaacaa gagaaatcgc acaaaaagtt gggatcaccc agcccaatct
    15901 ctatcattat ttcggtgata aagaaaaact atatacggca gttttagaaa accacttaaa
    15961 agatgttgga aaagctttga gagaaatcgt tcaaacaagc gaaacgggat ttcaagtaac
    16021 cttgacaaaa atggcgcagt ttttgattga gacacatctg gtcgatttgt ttctgatgct
    16081 tcacgatctt gaaagcaatc tttcaaagga aacaagagat catcttttct ttctatggaa
    16141 gaaaaattat cgtgaacctt ttgaagaaat cttttcagca aaccaatctg tgctaagaaa
    16201 tgggatttca aaagaaatcg cagcaaggca ttttttctta gtgttagcac cttacatcac
    16261 tcggtcgaaa agacctgtgg aagaaccgct aaatgtggcg caattgatcg atttatattt
    16321 gaatggtgta ttaggagaag cgtaaagctg ttatataggg agaaaatagt taggtgaaga
    16381 agcaagatat gacgaagatc gttatatctt gcttctttat ataggtagtt tttcataaat
    16441 acaaatttgt tattctctca tgattatcag ggtatatttt tatttattat gtcggtgttc
    16501 caggaagtgt tttaatttaa taacgaagaa attaattgtt tgtagaatga aggaaactat
    16561 ttattgggta gatgaatcac cacgaatagt caaaaggaga tttcaggata tgccaaaaat
    16621 gaaaaaaatc attttagcag taatgttatt ttctacggtt atatctttta cagccccttt
    16681 tttgcttact taagctgatt ttaagttttg gcccttagtc tggatctacg aggtgagagt
    16741 caagaatgaa aaaaataatt ggagcttttg tatttgcaat cattatcatt attatctgtt
    16801 ttcaaataga tgtttttggt caaaaaaaga tagttggaca aacggtcagc agtacgaaca
    16861 gtacggttac aaagacaaag gaatcatttg atgatacaga acaagagaca ttaatcaaaa
    16921 tcggaaaaaa tgaccgtaca ctatatacag atgactcgct aactacccca cttgcaacta
    16981 taaacggcgg agagctggcg gagtttcttt ctgaaacaaa aaatgcttat cagatcaaga
    17041 caaatgatgg ctatactgga tatttagccc tcgaagatgg aaccaaagtc acaaagaata
    17101 tacaaacaaa accggaagag ctatctgatg cagtgattgt attagatcct gggcatggcg
    17161 gaaatgatac tggggcatta agcaatgatg aacaaacaga agaaaaagat ataacattaa
    17221 gtacagccat aaaagtaaaa aaggcgctag aggatgctgg agcaacggtt tatttgaccc
    17281 atactacaga tgaattagta cagcttggag atatatgcga ctacagtgaa gaaaaaaagg
    17341 cagatgtctt tatcagttta cacgcagact ctacggaata tgcgaatgaa gcaactggaa
    17401 tcacgacata ttattactat ggacaagagg aaacattagc acagacaatt gctgactcct
    17461 tcactgatct gcctctggat tcaagaggaa tctccactgg gaactatcaa gttcttcgag
    17521 aaaatcttca accttctatt ttaatcgaga tgggttatat gaataatgat agtgatttaa
    17581 aggaattagt aacagacagt tatcagcagc agattgctgc aagtttgaca caagcattga
    17641 ccacttattt tcagtaataa ataagcccag ccaaacaaaa atagctcctc atatttttcg
    17701 tttggccggg cttattatcg cagatgcaag cttttaaaca ccgtttaagg aagaggtcgt
    17761 gagcctgccg ttcaactctg agtcacaagg agcaatttca aaaaacagct tctcatgttt
    17821 gatgactatt gtgacttgtg accgaggagt tggcatgaga acaccgttta agaaaaaggt
    17881 tgtgaaatca acgttttgtt ctgagtatta gagaaacaaa cggaaaaata gttcctcata
    17941 ttttgccgtt tgttgagcta ataccgagga tcagatgatt gaacaccgtt tattcggaac
    18001 aagggactgt gacatgactt ttgtcacagt tccattattt ttttagtagc ttcaaagctt
    18061 tttctgtatt ggcaaaatga agtttggcat atttttcaag cataggcatg ccgcctttct
    18121 ccaatagttt tgccgctact tggtcagatt tcgttccagc tcctgacgga agggtgattc
    18181 cagcttcagc tgatgctttg tctaactctt ccaaaaagct ggcacgactg atgatagaag
    18241 cagcagcaac agctaaatga tattgttctc ctttagtaac gaagtacagt ttttcttgta
    18301 attgattttt ttctaaacgc gcatattttt taaaatttgc ttccggcgta aactgatcga
    18361 tcaaaatcgc ttctggtttt tctggctcga ttttttttag cagcaaatgg atcgcttgat
    18421 tgtgcaaagc gactttcatc cgaacggcat tgtattttgg ttggatctca ttgtattttt
    18481 ctggagataa gacaagcagt ttatagggga tggttttttt taagatgtca gcaagctgga
    18541 tgatttggct atcttttaac tcttttgaat ctctaacacc aagtttacgt aattctgcaa
    18601 gctgatcacg tttcacataa gcagcacata ctgttagcgg accgaaatag ctgccatttc
    18661 cgacctcatc actacctaaa acggataatt gactgatatt ttttggcaaa gaagaggctg
    18721 tttttgctgg cttggcttgt ttatcctgtg cctcccccca tttatcagct tctattgctg
    18781 cattcgtacc ttgaaacatc acttttccgg atgtataagc ggtaatggtc gtcccgtctt
    18841 tttttgcagc aaagctagta taaggaatct gtttatttaa caaataagag cgatagtatg
    18901 ctttcatttg actaagttgg tcgtttgttg ctttgataat gacgttgctc ataaaattct
    18961 cctcgttttt atacataaaa ctataccgca actcacgact tttcgttttc taagagattg
    19021 cgagacatag cattttttta aagtttatca tataatagtt aagtatgtga aaaatatttg
    19081 tggagaacgg tgaaagaacc aacttcatgg tacactaaat aaaactttca tcaaatagga
    19141 ggaaatgatt cgtatggcgc acgaaaaaac acgctacaaa gcggtgattg ccaaccaaac
    19201 atatactatt atcggacggg aaaccaagca tcatatggat atcgtaacga agctgatcaa
    19261 tgaacagtta gcagaattga aacaattatc accacaaatg gataatgaac aagcagcaat
    19321 cttgatggct gtcaatgcgc tttcggatca attgaagaaa caagaaagaa tattggagct
    19381 ggaagaagaa acagcagaat taaagaaaaa aatgatcaaa tttacagagc ttgaaaatcg
    19441 tgtgaaacgc attgaagcaa tcgagaacga agctcgggaa gtattgaaag aaaatggtca
    19501 agcagatcat accattaaaa atcatgtgga agcacaacag attttaaacg agaaacgaaa
    19561 aggacaaatt aaacaaaaat cttctaatta agatcctttt atcaaaataa gaaaaattta
    19621 aaaaagttag aagagaaagg aacgtagcat gctgagtttg ctgattttat ttttattgct
    19681 aattgcgttt ttttctggag cacgtcgcgg gtttgctttg caaatcatat tcgcgattgg
    19741 atatgtattg tcatttattg ctgcccagca tttttacaaa ccattagcaa gtcatttgga
    19801 attatacatt ccatacccag ctgtcacccc aacatcaaag ttagcatttt ttgatcaggt
    19861 atttgccttt cacctagatg aagcttttta tgcaggaact gcttttctga ttattttgct
    19921 aattggctgg ctgcttaccc gatttgtggg ggtttttgtc catggattga cgtatgtgcc
    19981 gatcttgaga caggtagact ggattgcagg aggcatcctg tcactaatca tggcttacgt
    20041 tactatattt ttgattttgc agttacttgc ctttgttcca ttagatgtgg tccaaaatca
    20101 attcggcgag catagtttcg ctcgttttat cgttgagaga acgccatttc ttaccaacaa
    20161 gatgtatgag ctttgggtaa cgaatatttt gaattaagga aaatagtgct ggaaaatatt
    20221 tcgttttttg tacagagtag tttatttaag aaggctgcgt agcgtatgtc cggcacagct
    20281 cttttttcat agtgaaagat aaggtgataa aatgaataac cgaatccttg aaacattgga
    20341 atttgaaaaa gtcaaacaga tggtcagaca atttgtagtg actgcacaag gaaaagaaga
    20401 actggcagaa ttggtaccag tgagtgagaa acaaacgatc acaaactggc ttcaagaaac
    20461 tgaggatgga ttgaaagtgc aacgtctgcg aggcgggatc cctataccta aaattgaaaa
    20521 tatccgtccc catatgaaac ggattgagat cggagcagac ttgaatggag tggagcttgc
    20581 acaagtttcc agagtattgt ctacgacaag cgaattgaat cgtttcattg atgatctttc
    20641 tgacagtgaa atcgaatttg cacgtttgta tatgtgggca gatcagttag tcacgatccc
    20701 tgttttgagc cgccgtttaa aggaagcaat cgatgaagac ggacgcgtga ctgatgacgc
    20761 ttctcctgag ttaaagagca taagacagaa cattcggcga agtgaacaag ctgttcgtga
    20821 acagttagat gggatcgtcc gtgggaaaaa tgcaaagtat ttaagtgatg cgatcatcac
    20881 catgcgaaat gaccgctacg ttatcccagt caaacaagaa taccgcggtg tatttggcgg
    20941 tgtagtccac gatcaaagtg cttcaggtca gactttattt attgaaccaa aacaagtggt
    21001 ggatttgaat aatcgtttgc gtcaatacca aatcgctgaa cgaaatgaga ttcaacgtat
    21061 tttaagtgaa ctttctgctg aacttgttcc ccatcgtcaa gaaatcatcc acaatactta
    21121 tgtgattggt aaaatggatc tgatgaatgc aaaagcacga tttggtaaag aagtaaaagc
    21181 catcgtgccg ggaatcagtg aagacaatca tgtcgtcttg aaacaagcaa gacatccact
    21241 gatcgatcag gaaaaagtag tttctaatga catcacaatc ggaaaagact atcaagccat
    21301 cgtcattaca ggacctaaca caggtgggaa aacgatcacg ttgaagactt taggtctgtt
    21361 acaactgatg gggcaagcag gattaccgat tcctgcaggt gaagaaagtc aaatcggtat
    21421 ttttgaggaa gtatttgcgg atataggaga cgaacagtcg attgagcaaa gtctatcgac
    21481 cttttcttct catatgacga acactgtaga tattttatca aaagtcaatg aaaaaagtct
    21541 ggtattattt gacgagctcg gtgcaggaac agatccgcaa gaaggtgctg ctctagcaat
    21601 cgcaatctta gatgatcttg gtaaaaagtc tgcttatgtg atggcaacaa cgcattatcc
    21661 tgaattgaaa gtttatggat ataatcgggc aaatacgatt aatgccagca tggagtttga
    21721 tgtggataca ttaagtccaa cgtatcgttt gctgattggc gttcctggac gaagcaatgc
    21781 gtttgaaatc tctagccgat taggtcttga tacagaagtt attgatgaag cgaagcagtt
    21841 gatgaatgat gagagccaag atttaaacga aatgatcaca gatttagaga atcgccggaa
    21901 aatggcagag accgaatatc tggaaatgcg tcattttgtt tctgaggcac aagaacttca
    21961 cgatgatttg aaagaagctt acagctattt ctttgaagaa cgtgaaaaag aaatggaaaa
    22021 agcaaagaaa aaagcaaatg aagtcgtgtc agaggcggaa gaaaaagcag aaaaaatcat
    22081 tgctgatatt cgtaaaatgc aacagcagat tgggcaagga aatgtgaaag agcatcagtt
    22141 gatcgatgca aagacccaat tagcaaatct acatcaagaa gaaactttga agaaaaataa
    22201 agtattgaag aaggcgaaag aacaaaaaac gttgaaaccg ggagacgaag tgttggttac
    22261 gacttacggc caaagaggga cgcttttacg taaaaacggg aatcagtggc aagtagaaat
    22321 cggtatatta aaaatgaatg tgtccgaaga cgagctgacg cctgttgctc ctcaaaaaga
    22381 gccgacacaa cgagttatcc atgctgtacg atctgaatct agtagtcacg tgccgaatca
    22441 attggacctt cgaggaaaac gatacgaaga agcgctaagt gaggtagatc aatatttgga
    22501 ttctgccatt ctagctggtt atcctcaagt gactattgtc cacggaaaag gaacaggcgc
    22561 acttcgcaag ggaattactg attatcttaa aaaccatcgg agcgtcaaaa gctttgagtt
    22621 tgcaccggca aaccaaggcg gaaatggtgc gacaattgtt aaatttaagt aaagcttact
    22681 aaaggtaagc agatggttcg ctatccaagt atagaaatgc tatactagtc atagatttta
    22741 aaaatggagg tctttgatat gtcacaagta attacagata agacatttaa tgaagaaaca
    22801 gataaaggac ttgtattgat tgatttttgg gcaacttggt gtggtccatg tcgtatgcaa
    22861 gcaccgattt tggatcagtt ggaacaagaa tatgatgaag aagaattcag aattgctaaa
    22921 atggatgtgg atgaaaatcc agaaactcct caacaattcg gtatcatgag cattccaaca
    22981 ttaatgttga aaaaagatgg tcaagtagta gaaaaagctg ttggtgttca ttcaaaagaa
    23041 caactacgtc aaatgatcga tcaatattta taaaaactta cagcaaaaat aagctgaggg
    23101 tgggactgaa tccagttccg tcctcggttt ttttgcggtt gagttaaggt tattcaaatt
    23161 aatatttttt gagtataatt acgttgtcag gctggattgt ttttttgaca gacatgctat
    23221 aataacttta attgaatatg aaggggagta ccagatgaac gaacgaatca aaaacaaatt
    23281 agctttactt cctgatcagc ccggctgtta cttgatgaaa gataaaaatg gaacgatcat
    23341 ttatgtcggc aaagccaaaa tattaaaaaa tcgtgtccgc tcatatttta ctggtagcca
    23401 taatacgaaa acggaacggc ttgttagtga aatcgaagac tttgaataca tcgttacaga
    23461 atcgaatatc gaagcattgc tcttagagat caatctgata aagaaaaatg atcctaaata
    23521 caacattatg ttaaaggatg ataaaaccta tcctttcctt aagataacga atgaaaaata
    23581 tccccgtttg gtgattactc gtaaggtact aaaagacaaa gcactgtatt ttggaccata
    23641 tcccgatgtc ggtgcggcga atgagacaaa gaagatcctc gatcgattat ttcctctgag
    23701 aaaatgcaaa cctagtcaga cgaaggagcc ttgtctttac tatcatttgg gacaatgcct
    23761 gtgtccttat tatttcgacg tagatccgaa agtctatacg aaaatcgttg atgaagtgaa
    23821 aaacttttta aacggaggac acgaaaaaat cgagtcagag atccatcaga aaatggaaaa
    23881 agctgctgaa aacatggagt ttgaaaaagc tgcagaatac cgagaccaga tccgtgccat
    23941 tgagacaatc atgactcgcc aaaaaatgac aaatactgat ttactggatc gagatgtatt
    24001 tggttatgcg attgataaag gatggatgtg tgtccaagtg tttttcgtaa gacaaggaaa
    24061 gctaattgaa cgagatgttt caatgtttcc tttctataac gaagcagaag aagatttctt
    24121 gacttacctc ggtcagtttt atcaggaaaa tgagcatttc attcctaaag aagtcttgat
    24181 tcctgatacg atcgacaagc ccagtgtaga agctttgtta gctacgaaag tgctgcaacc
    24241 aaaacgagga gaaaagaaaa aattagttga attggcgagt aagaatgcct cagttgcttt
    24301 gaatgaacgg tttgatctga ttgccagaaa acaggaacgg acgatcggag cagttgaacg
    24361 tttaggaaac gcgatgaata tcccgacccc aatacgtatc gagtcttttg ataactctaa
    24421 tatcatgggg acgaatcctg tggctgcgat ggtcgtattc attgatggaa aaccggcaag
    24481 aaaagaatat cggaaatata aaataaaaac agtcaacgga ccggatgatt atgcatcaat
    24541 gagagaagtc atctatcgca gatattcgcg agtgattcgc gaagggttgc ctttacctga
    24601 tttgatcttg atcgatggag gaaaaggaca agtagatgtg gcaaaagatg tcttagaaaa
    24661 tcaattagga atcgatattc ctattgctgg catggtaaaa aacgataaac ataaaacaaa
    24721 tgagttgcta ttcggtacag atttggctgt agtaccgctt gagcgaaatt caccagagtt
    24781 tttcttactc cagcgaatcc aagatgaagt ccatcgattc gcaatcactt tccatcggca
    24841 gctacgcagt aaaaacagtt ttgcttcccg attagatgaa attgaagggt taggaccaaa
    24901 acgaaaaaaa atgctgctta aagagttcaa atcgttgaaa aatatcacag cagccacgac
    24961 agaagaatta caagaaatcg gactgccgaa aaatgtcgca caaaacgtat ttgataaatt
    25021 acatcaggaa tgaggaaagg gcactttaca aagaaagtaa agtgtcctat cattttatgt
    25081 acaacaaaaa gagcgtttac gttttgttgg taaataataa atcgcttacg tctggtgtct
    25141 aaggatatat gtgatttctt gaagaaggag tgaagaaatg ttagaagtca aggatcttgt
    25201 taaaactttt gggaactaca cagctgtcga tcatgtctct ttccaaatcc ccgatggaaa
    25261 aatcatgggg ctaattggcc aaaacggggc tgggaagaca acgacttttc gtttgatctt
    25321 ggattttttg acacaagacc aaggagaagt gttatggaat ggccatcatc ttggggagaa
    25381 agattatgat gtcatcggtt atcttccgga ggaacgggga ctttatccga aagtaacgat
    25441 tcaagaccaa ctgctttatt ttgcagaact tagagggaaa tcaagaaaag agattgaacc
    25501 aaaaatcgac ttttggatgg aaaaatttca ggtaaaaggg aaaaagacgg ataaagtaaa
    25561 atctttgtct aaaggaaatc agcaaaaagt ccagctgatc gccacgttaa tccatgaacc
    25621 taaattgatc attttggatg agccattcag cgggttagat ccggtaaatg ctgaattgct
    25681 aaaagatgga atattggaat tgaaaaaaca tggttcatgt gtcatttttt ctagccacaa
    25741 tatggataat gtagaaaaaa tctgcgatca tttgatcatg ttgcgaaacg gaaaaatggt
    25801 tttgaatgga aaagtacatg agattcgtga atcatttggc cgtacgaagt tattcttgga
    25861 atctggttta agccaacagg aagtagaaga aatcgaaggc gtgaaaaaag tgatccttca
    25921 tgaagacaat acgttagaga ttaccttaga agatcctgaa gctgggaaag aaatctttac
    25981 tcgtgcgact cagttcggct acattcctat gtttaaccag caaccgccaa ctttggaaga
    26041 gattttcaaa ttgaaagcag gtgaaatcga tgcgtaagtt ttggattatt accaaagatg
    26101 tctatttaaa aaatgttaag tcgatttctt tcttgatcat gatattagtc ccttttatca
    26161 tgctgggtat catttatgta gcaggaaact ttgctcaaca aaatagtgat gtcaataaaa
    26221 tcggcgtatt ggctgataat gagcaaatca gtcagcaaat gagccaagtc aaaacagatg
    26281 attttgagtt caaagcagtt gattctgaag aaaaagcaca aaaagagctt tcggaagaaa
    26341 aaatcgatgc gtatttagta gtctcaactg agaatgataa gataaatggg atgctttata
    26401 gcgaaaattc attaggacaa agtacacaac tactgataca acaacaactg actggcttgc
    26461 aatcgatgct tcgagcgagt caattaggca tttcaccaga agaagtagct agcttgagcc
    26521 aacaagcaag tttgactcga caaaaagtca attttgattc aaaaggaaaa atgacagtcg
    26581 gagaagataa ctcgacagtc caatacgtcg taagttatgt agcaacgatt gtcttgttca
    26641 ttattatttt gacgtatgcg cagatcattg cacaagagat cgcttctgaa aaaggcacgc
    26701 ggatcatgga agtgatcctg tccagtacga ctgcacaaaa gcatttttac ggaaaattaa
    26761 caggtgtttt acttgttgca ttaacgcaaa tggcattata cggtattatt tttggtatag
    26821 gatacagtca attcaaagat atggaaatgg tcaaaaatat attagacggt atctcattgg
    26881 ataacttgat tggaccattc ttgtggtatt ctcttctatt catgatcttt ggcattttga
    26941 ttttttctgt tttagcagct ttatgtggct ctttggtcaa caaagcagaa gatacagcaa
    27001 aagcaatttt gccagtgact tatctatctc taggcggata catgcttgga ttgatactag
    27061 gagcttcaga tccaaacaat atcgttattc ggatcacttc gtatattcca ttcttatctt
    27121 catatattat gccgattcgc ttagcaaatg agacagtcgg tatgggtgga gcattgattt
    27181 ccttgttggt acttgtagcc gtaacattct tattgatgat cttgtctgca aacatgtaca
    27241 aatcgaatgt actcgtttat agcgaaggcg gattatggag ttcattgaaa caatcgattt
    27301 caattatgag gaatgaacga aaaaaagcat aaaacaatcc taaaatacag aaggaagctg
    27361 gacatctatt gttcccagct tctttttgta tagacctatt ttatttattt tactatattg
    27421 gatatatatt cattgaaaat agtgtataac aactttataa tattgagtat accaattttt
    27481 ggaggcgttt taatggaccg ttatttagat ttactttcag aaaaattccc ctcaacggag
    27541 gatgtcatca cggaaatcat caatctcgaa gcaattttgc agcttccaaa aggaacagag
    27601 ttatttttga gtgatataca tggcgaattt cctgcttttg atcatattct gcgtatcggt
    27661 tcaggaaatc ttaaagaaaa gatcaaagac cttttttctg aacaaatgac ggaaacggat
    27721 atcagccaat tcactatttt tacggcttat ccagaatatg cattaacgac tgattggtac
    27781 aaagaacaag ataaatcgca gctgatccat catttgatcg atcttttacg gtttacgaca
    27841 gtcaaatata cccggtcaaa agttcgaaaa ggattgccga aagaatatag ctatatcatt
    27901 gaggaactct tgtatatcga tgaccgtatc aatggaaaaa aagactatgt aaaaaaaatc
    27961 attgaacaac ttgttttgat gaaggaagaa gaaaaatttt taacgaaact tgcacagacg
    28021 atccaaaaat ctgtcatcga tcatttgcat atcgttggag atatttttga ccgaggaact
    28081 caagcagcca aagtgatgga tcgaatcatg gatttttctt ctgtagatat ccaatgggga
    28141 aatcatgata ttttgtggat tggtgcgtat tgcggctctg aggcatgttt actgacactt
    28201 ttacggattg cagcacgcta taattattta tttgaattag agaaagaata tgggttgaat
    28261 ttacgtccat tgttttcttt tgctcatcaa acttatcaga agaatccagt atttgcgcct
    28321 aaaaacagta aaaatctatc cgaaagagag caagaagaat tagaaaaaat ccatcaggca
    28381 ctttctatcc ttcaattcaa atcagaacat cagttattgg atcgtcgacc tgaatttaaa
    28441 atggacgatc gtaaattgct ggaaaaaatc gactatgaag catcaacgat tgatttagaa
    28501 ggtcagctct atgagttgaa aggaacttgt tttcaaacga tccagccaga tgatccaaaa
    28561 aaattcttgg aagaggaaca aaaagtgatt gatagtttga tgtattcatt ccagcactct
    28621 ttgagaatgc aaaaacatat gacgtttttg atcgaaaaag ggggaatgta tctcacgaac
    28681 aatcagcatg tgctgtttca tggctgtata cctgtagacg agaagggaca gctcttagca
    28741 tttgaattag accaatctta tcgcggaaaa gaactgctgg gcttttttga aaaacagata
    28801 actgaaagcg caaaagatct gactgcaaaa gaagatttag caaccgattt gatttggtat
    28861 gcgtggagtg ggccattttc gcctttattc ggcaagagta aaatggcgac ttttgaacgt
    28921 tattttctca cagagcctca tacgcacgtc gagaaaagca atccttatta tcacttgcga
    28981 gatgaagagt ggttcagtga aaaaatttta gcagaattcg acacaaaaga agaagggtca
    29041 gcaatcatta atgggcacac gccagtaaaa gtaaaaaaag gggagtcacc gatcaaagca
    29101 aatggaaaag tatttgtcat tgacggaggt ttatccaaag cttatcaaaa aacgacagga
    29161 attgccggtt attcactatt atgcaattct tatggtttcc aaatcgtgac acattatccg
    29221 tttttgccta ttgaacaaca atttgcaaaa aaatccgacc aaacaaatgt gaaaaaagta
    29281 atcgaacagc aactaccacg caaactcaat cgtgatacaa caaaaggtat ggaactgcag
    29341 cagcagatta cccagctcaa aaggctgttg gactatcgaa aggacgattg agaagaattc
    29401 cagtgaacat tgttccatag actggagact gagacaaaag tcgtgtcgca gtctcttttt
    29461 ccggataaac ggtgttcttc tgccaactcc tcggtcacaa gtcacactat tcaacaaaca
    29521 tgaaaagctg ttttttgaaa ttgctccttg tgactcagag ttgaacggca ggctcacgac
    29581 ctcttggtat aaacggtgtt caaaagccag tttctcggta ttaagccaaa tttatgcaaa
    29641 aatgtgaaaa gccattttca caaaattttt cttaatactc gaaactaaac agctttttca
    29701 caaccccctc agaaagtcta ttaaaattca ttcacaaaga aatcgaataa gcgctgttca
    29761 gatggatgta cacggtgggt aagttccgga tgccattgaa caccgaggat tttatgcata
    29821 tcatctttgc tttgaaccgc ttcaacgagt ccatcgttag aaaaagcgat ggcttttaat
    29881 aaaggtgaaa gatctttgac tgcctgatga tggtaagaat tcacaaaata tggatcgggg
    29941 agaaggttgc ttaacaaact gtctgaaaca atcttgactt cgtgagtcgt aaattgcggg
    30001 gcagtgggct gttgttcatg ttttactgac cactttggat ataatgaaag atcttgataa
    30061 agtgtacctt ccaaaacgac attcaatagt tggagtcccc ggcagactgc aaaaattggt
    30121 tttctttgtt tcaatgcttc caagatcagt gcctgttcaa attggtctcg ttgaatattt
    30181 gtttccgtta gctttggatg aggatcttcc atgtaaagtt gtggactgac atcttgacca
    30241 cctgcaagaa taagtttgtc gatttgtcca atcagttgag gagctgaatc tggagacatg
    30301 actggcagaa taagaggcaa tccttgagca tctaaaactg catccacgaa tccttgcggt
    30361 gtatagctca cttgatttcc ttggaaagta tctgttgctc gaatcagttg atttccggca
    30421 atgccgatga ttggtttcat ttgtattccc cctaaagtag acttattgta acaaggattt
    30481 aaaaaaaatt aaactatgta agacttgccc ctatacttat ctgttcttgc tgtgttacta
    30541 tctgtaaaga gaaatttcgt gtgaaattca tgacgatagt tgaaaatatt gcttaatttg
    30601 ttataataaa aagtaagcag aaataaaaag gttgggcgta agcttcaagg atctagttct
    30661 tcaaggggtg agaaggacat tttattgaag gggcctgcct atactaagtt atctttagag
    30721 gtacatactt tcaatgaaaa aagaaagcat gtacctcttt tttaaatcga aaggaagtga
    30781 agcaagtgtt agaagctttg gaacaaatcc gaaaagcaga agaagaaaat gaaaagcgtg
    30841 ctcaaacact gcggcagcag ctgaagatct atgcaacaga aaaagagcag gaattggaaa
    30901 cgatccaaac aaactatcga caacaactaa aacagttggt ggaagaaaaa gaacaatttg
    30961 aaatacaaga attagaaaaa gaaaaaatga aattgaaagc agaagcagac aaggtgcaag
    31021 caactttgaa agatcaatat caaaagcatc agagtcaagc gatcggtgcg atcattgaaa
    31081 gggtgaaaga aacttatggc cgtcactaag atggaaaaag tgaccctcat ttcagataaa
    31141 agaaatcagg agaggatttt acaagcagta caaggaatcc aaaacgttga aatccgggat
    31201 ctgtatcaaa atagaacaaa caaccagtgg gtggagaagt attttccaga taccacgatt
    31261 attgataaag aagcaaaatt aagcgcttta ggtaatcggt tgaaagacat ccaagaagct
    31321 atccaattca tacaacacca cggcgatagc cgtcaaaaga aactccattt gaaacgtcaa
    31381 gaactttcct tacaagattt ggaagccaat tattccgaag aaaatttcat aaataaattg
    31441 aaagaagtcc ttgatttaaa agaaaagtgg gaacaactaa ctgaagaacg tgatcgttta
    31501 gaagaagagg aagaatggct tcttcattgg caacagctgg atattgctcc aagtatgtat
    31561 gcaagtaaga cgaccatttt tgagatgggg actgtgagtg cagcaaattt tgaaagtttc
    31621 aaggaagaac tgagtaagct tgatgacgtt tatatggaag aaatagatta tacgccaaaa
    31681 caagtttatc tttcgtatgt tgctttaaaa aaatcagttg ctgttataga agaaattgct
    31741 gggaaatacg ggttcaataa agaaacgtat ccttatgaac aaaatcctaa agaaaaactg
    31801 caagagacca agcagcaatt atccattgtt cacgagcagc aaaagaagat aacgacagcg
    31861 ttaggtcagt gcagtggcta tatccaagaa tttgaatggg tagaagaagt cacacttgcc
    31921 gttgcagaac gagaaaaaat aaaagaacgg tttatccatg cgacttatct gatcgttttg
    31981 caaggttggg tagatgcaga agaaaaacag aatttgctgc atgtgttaaa cgaaaccgta
    32041 tcagaagaag atatatacgt cagtttcgaa acaccaaact cagaagagat tcagcaggaa
    32101 gtccctacta aattgaaaaa ccatccgctt gtcgaacctt ttgaattgct gactgaaatg
    32161 tacagtctgc caaaatatga agaagtagat ccaacacctt ggatgacacc attttatctt
    32221 gtcttcttcg ggatgatggt cgcagatgtt ggttatggtt tgctgatgct cattgggaca
    32281 atactagctc aaaaactact tgttctacca cgcgggatga aacgtttcgt aaaattcttt
    32341 gagattctct cgataccatc gatcatctgg ggattgatct acagttcatt tttcggacag
    32401 tcattaccaa aagaactgtt tggcatccgc ttgcctttcc caatcctatc tacgacggat
    32461 gacgtcaata cgattttgat tttatccgtg attttcggtc tgatacagat tttggttgga
    32521 ttatttgtgg cagcaaagga aaatatcagg agaaaagatt acttgagtgc tgtcagtgat
    32581 ggatttgcct ggcaagggat cctaattggg atcgttattg cgttggttgg tgccatgttg
    32641 ataaaaaata cagcatttgt ttaccttggc gctgggctat cgatcattgc tgccttatgt
    32701 atcttgatcg ttccgattat ccaaacgccg tctaaagtca aaggtgctgc caaaggtgcg
    32761 tataatcttt acggactgac cggctatatc ggtgatttag tcagttatac acgtttgatg
    32821 gcattgggta tttcaggcgg aagtattgga gcggcattca atatgctagt tgctttcatg
    32881 ccgccagctg ctcgattcag tgtagggatc cttttgatca tcgctttaca cgcactgaat
    32941 atgtttttga cactgctaag tgcctatgtc catggcgcac gattgcagta tgtcgaattt
    33001 tttgggaagt tttatacagg aggcggacgt gcctttgatc cgctgaaaac ggcggaaaaa
    33061 tacgtcaaca tcaatcacaa gaaaaaaaac aataaataat tggaggattt ttatataatg
    33121 atggattact taattagtca aaatggtgga atggtatttg cggtattagc gatggcaaca
    33181 gcgacgattt tttcagggat cggttcagct aaaggtgttg gaatgacagg tgaggcagca
    33241 gcagcattga caacaagtca acccgaaaaa ttcgggcagg ctctgattct gcaattgctt
    33301 ccaggcacgc aaggtttgta tggattcgtt attgcctttt tgatttttat caacttagga
    33361 aatgacatgt cagttgtaca aggactgaac tttctaggtg cttctctgcc gattgctttt
    33421 acaggcttgt tttcaggtat tgcccaagga aaagtcgcag cggcaggtat tcaaatttta
    33481 gcgaaaaaac ctgaacatgc tacaaaagga atcatttttg ctgcgatggt tgaaacatac
    33541 gcgattttag gtttcgttat ttccttccta ttagtgttga atgcctaagc ataaaggagg
    33601 aaaagcagat gaacgccatt gaaaaaatca tctctcagat gaacgaagca gcagagcagg
    33661 aaagagcggc gttagaacaa gaagagcgaa tgaagatcga tcaaaacttt gaacaaaaac
    33721 gtacgcaagt tgaaacagaa catcaaaaac aaaaagaaaa acaaatcgaa ttactcgaaa
    33781 aaaaataccg tcagctaagg aatcgccagc aagtagaagt acgacaagaa aatttgaatg
    33841 cgaagcagga atttttaaga cgattgtttg cagatgcggt aactgaaatg gaaaattggg
    33901 atgaatctga acaaatccag tttatcaaaa atgcattgta ttcattacct ttgacaggaa
    33961 aagtagcatt cattgctgga gaaaaatcag cagcctatct ttctcagacg ttgttggatg
    34021 aatggaacaa tgaacttccc tttatgatgg ttttaagtga tgaaactgtt gctgatcaag
    34081 cgggttttct gataaacgat caaggagttc agtataattt tcttttcagc agccttgtac
    34141 aagatatcca aggaacgatg agttttgaga ttgccaatca attattcgaa taaggaggga
    34201 tcagtgtatg gaataccacg aactaaatcc cttgattcgc ggaagagagc ttgaactttt
    34261 gtccaatgag acatttgaac ggatgattca aacaaattct gtcgaagcac ttggtgaaat
    34321 attgaaatcc acgatttaca gtccatatat ttatgacgga tatgaatatg acttcgaaca
    34381 gaatctttta aaggagcaga gccgtctatt tcgctggtta aaggaatcag caccggagcc
    34441 tgaaatcgtt tggatctata cgatgcgtta tactttccat aatctgaagg ttttgacaaa
    34501 agctgagatg acaaatcaaa atctggacca tttgtatatc gacgatggat tttattcatt
    34561 agaaaccttg aaggatgcca ttcatacgca agtttcaaat gaacttcctg aaagcattat
    34621 ggacagtata cgagaagtac gtgattattt tgaagagtct accattcttc agggaattga
    34681 cgtgatttat gatcgacagt ttttgacaga gcagcgacgg cttgccgata agctaggata
    34741 tcaagaattg ctgacagaag tcattgcttt tattgacttg accaatatca caacgatggc
    34801 tcgaggaata ttgcagaaaa gaacatatgg gttcatgacg acggtacttt ccagctcggg
    34861 aagtatcgaa aaagaagtat ttttgaactt tgtagataaa gatatggaag attacacgaa
    34921 ttttctatta gagacagatt atgctgattt tctgcgccct gtcatccgtg agggaaatat
    34981 tgatctcgta aagcttgaac agttgaaaga tgattatcta agttcattgt atcaagaagc
    35041 acaaacccag gctttcggac cactgccact actagccttt ttgaacgcaa aagaggtaga
    35101 aagcaaaaac cttcgtttac tggttatcgg caaaagaagc catttcgaat cagaaacaat
    35161 cagaaagcgg gtgagacaag tctatgacgc ataagatcgg tgtagttggc gacaaagatt
    35221 ctgttttgcc attcaaattg tttggctttg atgttcgtta tggtacaacc aagcaaaccg
    35281 ttcgtcaggc aattgaagaa atggcaaaga atgagtatgg cgtgatttat gtcacagagc
    35341 aatgtgcagc attgagccca gaaacgatcg aacgttacaa ggaacaaatg atacctgcta
    35401 tcgtattgat tcccaatcat caaggatcat tagggatagg gctggaagaa attcaaaaaa
    35461 acgtagaaaa agcagtaggt caaaatatct tatagtatga aaaggagata gaaattttgc
    35521 aaattggaaa aatcataaaa gtctccggac ctcttgtaat ggctgagaac atgtctgatg
    35581 ccagtatcca agacatgtgt ctagtgggag atctaggagt tatcggagaa attatcgaga
    35641 tgcgtggcga tgtggcgtct attcaagtat atgaagagac ttctggaatt ggtccaggcg
    35701 aaccggttcg ctctactggt gaagctttat ctgttgaatt aggaccaggg atcatttcac
    35761 aaatgtttga cggaatccaa cggccattag atacctttat ggaaatcaca caaagtaatt
    35821 tcttagggcg cggagttcaa ttgcccgcat tggaccatga gaaaaaatgg tggttcgaac
    35881 caacagtaga agccggagaa acggtgagtg ctggagatgt tatcgggatc gtagaagaaa
    35941 caaaagttat aaaacataaa atcatggtgc caaatgggat aaaaggaacg atcaaaacaa
    36001 tcgaaaaagg ttcattcact attgatgaaa cagtatgtgt cattgaaacc gaaaatggtg
    36061 acaaaaattt gacgatgctt caaaaatggc cagtaagacg tagtcggcca atcaaagaaa
    36121 aattaaaccc ggatgcaccc atgatcactg gacagcgagt gattgataca ttcttcccag
    36181 tgacaaaagg aggagcagca gctgtacctg gtccttttgg tgcagggaaa acagtcgttc
    36241 agcatcaaat tgcaaaatgg gccgatgtgg acatggttgt ctatgtggga tgtggcgagc
    36301 gtggaaatga gatgacggat gttttaaatg aattccctga attgattgac cctaatacag
    36361 gagaatcgtt gatggaacgt acggtattga ttgctaatac atcaaacatg ccagtagcag
    36421 ctcgagaagc ttctatctat acgggaatca caattgcgga atatttccgt gacatgggtt
    36481 atgacgttgc tatcatggca gactccactt cacgatgggc agaagcactt cgtgagatga
    36541 gcggacgttt ggaagaaatg cctggtgatg aaggttatcc agcttatctt ggttcacggc
    36601 ttgcggaata ctatgaacga tctggaagag tcattgcatt aggatcagaa ggtcgcgaag
    36661 gaagtataac cgcaatcagt gctgtatccc cgtctggagg ggatatttcg gaaccagtca
    36721 cacaaaatac attgcgggtc gtaaaagtat tctggggtct tgattccagt ttagcccaaa
    36781 aaagacattt tccttcgatc gattggatac aaagctattc tctttattca acagaagtag
    36841 ggaagtatat ggatcatatc cttcaagctg actgggcatc catggtcacc gagggcatgc
    36901 gtattttgca ggaagaacag cagctgaatg aaatcgtgcg tttggtaggg attgattccc
    36961 tttcagataa tgaccgtctg acattagaag tggcaaaatc cattcgtgaa gattatttgc
    37021 agcaaaatgc ttttgatgat gtcgatacat tcacttcaag agagaaacaa ttcaaaatgt
    37081 tgaagatgat cttgaccttt ggtgaagaag gacgccgtgc gttgtcgtta ggtgcttatt
    37141 taagtgagat catggacggt accgtatcga ttcgtgaaag gatcggacga agcaaatata
    37201 tcccggaaac agaattagaa aaactagatc aaattattgg tgaaatcaag caaacgatgc
    37261 aaacgatcgt ttcagaagga ggcatgagca atgattaaag aatatcgtac gatcggtgaa
    37321 gtcgtcggtc cattgatggc ggttgaaaaa gttgctggtg tcaaatatga agaattaatc
    37381 gaagtccgta tgcaaaatgg cgagattcgt cgtggacaag tattggaagt ccaagaagat
    37441 aaagcaatgg tccaaatatt cgaaggaaca agtggtatca atttacgtga ctcttctgta
    37501 cgatttttag gccatccttt agaactaggt gtatcggaag acatgatcgg tcgagtattc
    37561 gatggtttgg gacgaccgaa agataacgga ccagagatcc tgcctgaaaa gcatttggat
    37621 attaacggag aagtaatcaa tccagtagct cgggattatc cagatgagtt tatccaaaca
    37681 ggtatctctg caatcgatca tttgaataca ttagttcgtg ggcaaaaatt accagtattt
    37741 tctggctctg gtcttcctca taaagaatta gctgctcaga tcgcgcgaca agcaacggta
    37801 ctcgattcat cagatgattt cgccgtagtc tttgctgcta ttggaatcac ttttgaagaa
    37861 gctgaatttt tcatggaaga ttttcgtcaa acgggcgcaa tcgaccgttc tgtcatgttt
    37921 atgaatttag caaacgaccc tgcaatcgaa cgtatcgcta ctcctcgtat ggctttgact
    37981 gctgcagaat atcttgctta tgaaaaggga atgcatgttt tggttatcat gacagacatg
    38041 accaactatg cagaggcatt gcgagagatt tctgcagcac ggcgagaagt tccaggaaga
    38101 agaggatatc ctgggtatct ttacacgaac ttagctactt tattcgaacg agcaggaaga
    38161 atccgcggac taaaaggatc ggttactcaa attccgattc taacaatgcc agaagacgat
    38221 aaaactcatc caatccctga cttgactggt tatattacag aaggacagat catcttgact
    38281 agagagcttt acaagagtgg gattcagcca ccaattgatg tactcccgtc tctttcgcgg
    38341 ttaaaagata agggaactgg acccggaaag acaagagaag accatgcagc tacgatgaat
    38401 cagctttttg cagcctatgc acaaggaaaa caggcaaaag aattagccgt agttttaggt
    38461 gaatctgctt tatctgatgt agacaagatc tatgcgaagt ttgctgagcg gtttgaaaaa
    38521 gaatatgtca atcaaggatt ctacacgaac cgttcgatta ctgaaacact tgatctaggg
    38581 tgggaactgc tatcgatgtt gccaagaacc gaattaaaac gaatcaaaga cgacatgctg
    38641 gacaaatatc tgccagaagg gaagtgagtt ctgatggcac gattgaatgt gaatccgacc
    38701 cgcatggaat tgactcgatt gaaaaaacag ttgacaactg ccacaagagg gcataaatta
    38761 ttaaaagata agcaagatga actgatgcgt caattcatcc tgttgattcg aaagaacaac
    38821 cagctgcgtc aagaaatgga agagtctatg acagaagcaa tgtctgattt tgtattggca
    38881 aatgcttcga tcacggaacc tttcatcgaa gaattatttg ttttgccggc ggaaaacgtt
    38941 gatttgtcta tcgtagacaa aaatatcatg agtgtaaaag tacctgtgat gaattttgaa
    39001 tatgatgaac agctgacaga cacacctttg gaatacgggt atttgaattc cagtgccgaa
    39061 ctggatcggt caatcgatcg gtttactacc ctgcttccaa aacttttgga actgacagaa
    39121 atcgaaaaaa cttgtcaact gatggcgagt gaaatcgaaa agacacgtcg gcgagtcaat
    39181 gcattagaat atatgacgat ccctcaatta gaagaaacga tctattatat tcgtatgaag
    39241 ctggaagaaa acgagcgcgc agaagtaaca aggttgatca aagtaaaaaa tatgggtacc
    39301 actgactaag tggtatcttt ttttaaaaga ggaaaacgat gattaaacaa agcaaacaaa
    39361 taaagaaacg tttgccggct gttcagcttc tcgcattagg attttttctc ctgattttag
    39421 ttggcggcgg cctacttacc ctaccatttt ttagtaatag tgggcatggg acaaatttta
    39481 tcgatgcgct tttcactgca acttctgctg tctgtgtaac gggattgaca acattgaata
    39541 cagcagagca ttggaacagt gctggtcaat ttttgatcat gcttttgatt gagatcggcg
    39601 gtcttggttt tatgatggtt ccgattctat gctttgcatt gataaagaag aaagtcagtc
    39661 tcagcacacg tattatatta aaagaagcgc tgaatctaga agaagtatca ggtgtgatga
    39721 agctcatgct ttacatccta aaattggcct ttgtgatcca attgattggt acggttgctt
    39781 tatcttttgt atttgtgcct gaatttggct ggttgaaagg tatctggtac agtttgttcc
    39841 atgcaatctc tagtttttgt aatgcagggt ttgatttgtt aggagacagt ttagccaatc
    39901 accagcaaaa tgtttatttg atcatggtgg tatctgcttt gatcatttct ggtgggttag
    39961 gcttcatcgt atggcgtgat ttactggaat atcatcagaa aaagaagatg acgattcatt
    40021 caaaaatcgc attaaccgtt acaggtaccc ttttattagg cggattcgtt gtgttctttt
    40081 tcacagagcg caatggggca acgctagtgg aagggaatat gtttcaacga ttggcaaaca
    40141 ccttttttat gagtgtgaca cctagaacgg ctggatatta ttcagttgat tacctccata
    40201 tgagccatgc aggtctgatt ttaacgatgt ttttgatgta catcggcgga acatctgggt
    40261 cgacggccgg cggattgaaa acaactacgt taggtgtgtt gctgatccag atgcgttcga
    40321 tgttgaaagg acgtacgaga gcggaagcat ttggccgtac catccgacaa ggcgcagtgt
    40381 tgcgggcact tactttattt tttgttacgt tgacgttgtg tgtagtcgcc atcatgattt
    40441 tatcggtgac tgaaacgata cctaaaactt ctggcatcga atatattgct tttgaggtct
    40501 tctctgcgtt tggtacagta ggtcttacga tgggattgac gccagacttg actgcaatag
    40561 ggaaattgat cattatagcg cttatgtaca ttggacgagt tggtgtattg actgtggtct
    40621 tctctctgat gacaaaagca caaagaacac aatcaaaata caaatatcca gaagaaagtg
    40681 tcatgattgg ataatttcat cttctgacag aaacctagtg atttagtaaa ataagaaaat
    40741 aaaataattt ttcttgcttc ttggatactt taagtgcaaa atgttcgttt attttttgag
    40801 gcactacccg ttgttgaacc tcgggtagtg cctctttttt attgaaagga atcaataaga
    40861 tttgattcct ttatttatca ataggttata attcatattt ttttattttt tcttttacga
    40921 tacttagtcc agaagctaaa atttcaaggt tattagagga tagggaaatt cgaacgaatg
    40981 catctggttg cgcacgtgta gtaaagcgtc tggaatgata aagtcggata ccgttagcaa
    41041 gaaagtcatt ctcaattatc ttagggtcaa tctcaggatc aactggaaga attcgatagt
    41101 aagggacacg attcgttggt ttttctactt tgaaaagaga atcaaaaagc tgattggctc
    41161 tttttgtaag acttaattta gtttctaaga tcctatttgc ttcatttgaa tggaggattt
    41221 gagtgatgac ttccacatct aatccagaag ttttcacatt tatattaaaa attgcttgtt
    41281 ctatcttgct ttggacatgt actggaacta caagataagc aactcgcaat ccagaacaaa
    41341 taaattttgt cattccagct aagtagattg tttggttagg taataaattt tggaaaggag
    41401 gaatgatcgt atgttgatag taagttgtta aaaatgaatg gatatcatct tcaataaccc
    41461 atagaccttc tttttcaatc acttcggcta attgctctcg tcttttgtga gagatttgaa
    41521 accctatagg attattacaa gaaggcataa gaaagacacc atgaatcttc ttttttctac
    41581 attcctgaat caatatatca ggtcgcattc cttgctcgtc aaaatcaatt ggaacgatct
    41641 ctaaatgata taactgagcc aattcaatga aattagcgta tgtatatcgg tctgttgcta
    41701 ttctatcacc tggagagaaa agtgcagaaa gtgtgatagc caatccattt tgaacaccgg
    41761 aaacaataga tagagattct aaggacggaa tgactccttg agtttttaac caatttgctg
    41821 cagtagtcaa ttgaaaattt gtgcctttgg gatcttggta attaagcaaa ccaacaagat
    41881 ttgggttatt actagttgat tgaataaacg ggacaacgaa tttattacat tcttcaaaag
    41941 aactaaccag accaaaatca atgatatgtt cggttacttc attggtagag atggtgatgg
    42001 actcaaagac attgggagaa ataaatgtgc cacttccaat attcgtatac agcaaacctt
    42061 tctcaattcc atatttataa gccttgccaa cggtagtaaa atttaaatct aaataatcag
    42121 ctagctctct ttgggagggt aactgagtgt ttttctgtaa tttattcttt aaaatatctt
    42181 gttctaattg ttgaatcaac gatcgataat aaggacgctt caatttttgt ttgtctggtc
    42241 gccaagttaa cgggtaattg tcataagaat taataggcat tttctccact ccttaaaatt
    42301 gtataccata caattttacc attgattgta ttgattttag aagttaatct tttgtgagga
    42361 gggaattgtc tatgaaaatt attaattttg aggaagtagt agcttctttt agttttgaag
    42421 aagcgattca agtaatgaaa aaatgttttt ttgattatga aagtggtaaa atcagccaaa
    42481 atcctcgatt ggtggaaatt ttacctgacg gaaaaggcaa aaatatgttt gcgatgatgc
    42541 ctgcttattt gggggagaac agatattttg gcacaaaagt cattaccgct tttccggata
    42601 atcatcagta tcatttacct tctcatttag gagagatttt cttgtttgat gctcaaaacg
    42661 gactacccaa agcattgata aacgcaaatg cagtcacctg gattcgaaca gctgctgtat
    42721 ctgcgctggc aacagattac ttagcaaatc cgcatgcaga aacacttgct ttaattggag
    42781 ctggtcaaca agcattgtcg catttgactg cgatttctac cattcgtccc atcactacag
    42841 tgtttgttta cgatatttta gaagaaagac gaaatacttt tatcgctaaa gctaaaaaac
    42901 agtatcctaa aataacgttt ataaatgcaa tatcagtaga agatgcagta cgtccagcgg
    42961 aaatcatttg tacgttgacc tctagtaaag aggcatttct tcaaaaagag tgggtaaggg
    43021 aagacgcaca tatcaatgct gtgggcacat ttactcctga tacaagagag ataagtacat
    43081 cgttgatgga aacttctcaa atctatgtag atgattactc agctgcctta acagaaagcg
    43141 gggacctgct tatagccatg gcagaaggag atttagagga atcttgtatt gttggtacat
    43201 taggcgaatt agtttctgag aaaaaaacat tttataaagg agaatcgagg ccgacgattt
    43261 tcgacgcagt tggtctagct attgaagatc tttgttgtgc agaatatatt tataacaaat
    43321 tagagaaagg ggctttaaaa tgaacttaga ttattacgta gttgatgcat ttgcagatga
    43381 ggtgttcaaa ggaaatccag cagcggttta tgtgctagaa gaatggctgc cagaaggtac
    43441 gatgcaaaaa attgccattg aaaacaattt atctgaaaca gcatttacag tgaagaaaaa
    43501 ccaagaattt gagcttagat ggtttacgcc agatcgagaa atcgatcttt gtggtcacgc
    43561 gacattagca acagcatttg ttttatttaa ctattacaaa atacctgatg aaacgattaa
    43621 attttctact caaagcggta atctatttgt gacaagaatt aaagattatt attacatgga
    43681 ttttcctagt attatgccga aaaaagtgcc gatattgtct gaatatgaag aagcgattgg
    43741 ggctaagatt aaagaagcct acttagcacg tgatttattt ttcgtattag aagatgaaaa
    43801 aacggtggct aaattgcagc cagattttac agctatcaaa aattttgaat taggaatagg
    43861 agtcatcgtg acagcagaaa gtgtgcaaaa agactttgtt tctagaacct tctttccaaa
    43921 attaacaatc aatgaagatc cagtatgtgg atcagcacat tctaatctta ttccttattg
    43981 ggcagaaaga atgaataaaa caaaattgtc agcttatcaa atgtcatcaa ggggcgggga
    44041 tctagaatgt gaattaaaag aagatcgtgt gataatagga ggaaaagcag ttttgttttc
    44101 taaaggtacc tcgtttattt gatttctttc aaatgaatta aaaataaatt ggtggagagt
    44161 ggcaacctta tagctacaat ggttgttaaa aaaagacgca aactttttca atttaatgaa
    44221 aagtttgcgt ctcattttaa ttattggtgt ttgtttttta ttcaatcaag agtaaaatgc
    44281 cccatactaa ctatcctcgg atgacttcga ttttataacc atcagggtca atgacaaagt
    44341 agtaagaagg cgcagtgcca ggtaatcctt ttaagtcagt aatctcaaga ccagcagctt
    44401 tatgttgctc gtgtaatttt tctagatcat cagctgctat agcgatatga ccatagccgt
    44461 ctcctagatc atacgcacca tgatcatagt tgtaagtcaa ctctaattca tagtcatccc
    44521 caggaagtgt caaataaaca agtgtaaatt tgttttctgg aaaatcacgg cggcggcttt
    44581 cttcaaaacc aaatgccttt tgataaaatt cgattgatgc ttcaagatct ttcactcgaa
    44641 cacatgtgtg agccattttc atagattcat ctctcctttt ccttttactc aatcataaca
    44701 aaaagaagta aggaaatagt tgatttgtgc ttgttcatga gaaatttctc atgtaaaatg
    44761 gatagaaaat aaaactgtta aggtggaaac tattcatgga taaaccaatt tttggtgaaa
    44821 aaaagcaatt tgctgattat caaacccgtt atgcagcata tattgtcata gaaagagatc
    44881 agcagattgc agtaatcgag gctccaaatg gtgctttttt tcttcctggc ggggaaattg
    44941 aaggagaaga gacgaaagaa gaagcgattg tccgtgagct gatagaagaa atgggaattg
    45001 tggcagaaat ttcttcttat ctgggagaag ctgatgagta tttttactcg aattatcgtg
    45061 caacttatta ttataatcca ggatatttct ttgttgctga ttcttggaag cgtataggag
    45121 aaccaactga aaagaccaat aaaatttggt gggtgagccc tcaagaagcg cttgtgaaat
    45181 tgaaacgtgg tagccaccga tgggcagtcg agagatggat cgaaaaaaat aaaaactgaa
    45241 agaaagcgag tagatcagct ttctctcagt ttttttattt attacagtac atattttttg
    45301 attgcttcag ctacaccgtg ctcgtcgttt gatgaagtga tgacatttgc tgcttctttg
    45361 acaagagaaa cggcatttcc cattgctact cctagaccag catattcaat catggataag
    45421 tcgttctcgt tatctccgat agccatgatt tcatcttgat caataccaag gtgctgtgcg
    45481 agattcgcaa ctgccgcacc tttacttgct tctttattta gtacttcgaa gtaatagggc
    45541 gcacttttga ccgttgtgta tttatcacgg aaagtttgcg gtaaacgcgc aattgctgca
    45601 tctaaaattt ctggttcgtc gatcatcatc attttgacga tgttcatgtc tggtgtcatt
    45661 tcttcaggtg ttcgatattt tagcggcatc ttgactaaac ttgcttcgtg gatcgtataa
    45721 gcgctgatat ctcgattagg cgtgtagatc gctttgtcat cgatcgaatg gagatgactg
    45781 ccgacacggc gtgccattac ttcgatctct aggaaatctt catgtgtcag tccgtgacgg
    45841 gcgatgattt tacctgattt cgtttgttgt acgagtgccc cgttataagt aataacgtaa
    45901 tcattatctt gaaaaaggtt caactcgttt aactgttcct ggacacctgg taagggacgg
    45961 ccagtgcata aaacgatcga aacacctttt gcaacagcag cggcaatcgc ttcttttact
    46021 tcttttgtta cttctctttt ctcatttaat aaagtaccat cgatgtcgat agcgactagt
    46081 ttgatagaca taatttcctc caatatattt taatttattt caatcaatgc gccattacgg
    46141 atatggctgg caaattcttg ataagtttcg tcaaataaat cataatgatc acgcaaggac
    46201 ggatcaacca tttcttttgg aaagaaaaat cgctcatctc cttgttcttg tcccgctaat
    46261 gcagcaacaa gctgactaac ctgtgataat tctatcaaag ttccgtcatt tcgcaaaatc
    46321 tcgatttgtg tgcgatgtct gccttgttct gggcgataaa aatcataagg tagatcgtag
    46381 ctagaattca cagcagtgta ataaacagga ttatatccga ccttttctac taacaaagtg
    46441 agttcttgga ctaattttga atcccggttg ccagaaaaag tagcagattt cagtggttta
    46501 cgattcaaaa aacgtttagc taaatcattt aaaataggat cagcagattc agaccattga
    46561 gtaaagtaag tgcccaagac cccatcatca agtttgaggt attcttctaa agtaaagctt
    46621 ccgtcaagaa atggcagaag taattgagaa tggagttcaa aactgctggc atcttcttga
    46681 tacaactcgt gtgcgcgatg aagcagatgc tccaaaatta cttccatacc acgggaaaca
    46741 ggatggaagt acacttgcac atacatttga tatcggctaa cgatataatc ttccactgca
    46801 tgcatgccac tcatggaaaa agcgatcccg ccttcataag gacgaatcac acgtaatatt
    46861 cgggtaagat caaatgttcc atattctgtt ccagtgaaat aagcatcccg aagcaaatag
    46921 tccattcgat ctgcatcgat ctgactagaa atcatttgga caacttgtgg gttggggtaa
    46981 gttttttgga tcacactagc taccttttca ggaaaacctt cttctactcg gttaagaatc
    47041 tgatacactt ctgtttctgg agaagtgatg atctctacag tgatggcttc atgattcgta
    47101 tggaagatat gttcaaatgt atgagagtaa ggaccatgac cgacatcatg caacagtgcc
    47161 gcacataaag tcaccagccg ttcctgatca tcccagcctc cgttgcctat tttttctttt
    47221 gaaaaatttc gagaaaagat atcgcagatt cgtcttgaaa tctcataaac gcctaatgaa
    47281 tgagtaaaac ggctatgttc tgctccgtga aaagtgaaag aagaagtgcc taattgttta
    47341 atgcgtcgta gacgttggac ttctttcgaa ttgatcaaat ctaagattac ttgatgctgg
    47401 acatggatat agttatggac tggatcacga aaaacttttt ccataggcag catttgatat
    47461 ttataagggg ttgtcataat gtagctcctt tatgttatta cgttgttcca ttctctggat
    47521 ttggttgttc cattgctcca gctggatttc tttttttagc cagtcggtag aatcctgtaa
    47581 atcacttgca gaatacagct tttcaaaagc ttttgcaaat gcttctttga cttcttttac
    47641 aaaaaggggt tggtcaagca gttcttcaag tgttgccatt gtagcaggat ccactgcagg
    47701 atagccattt gtaccaaagt cttcttttaa agcagattga taaaatcgct tcatgacttg
    47761 cccacgtttt tcctgattac cgttgacact taagtacatc ataacagaaa tcccattttt
    47821 gatccgacgc tgagcgatac cggcaaattt tttgccatgg atgcttaaat cataagttcc
    47881 aggacaataa gaatgggtga tttcgaatgc ttgtatcggt gcctttggaa aagcgtattt
    47941 agtcaatagg agcatttgct cgtaagcgtc atctatggtt attttttttg tgtcactttg
    48001 ggggaaaatc aaagaaatat tcaaaattcc ttcatctgat acgatcccta atccacctga
    48061 atttctgatc actggagcgt aacctgcatc ttccagtgtt tggatcccgt cacttagaaa
    48121 aggagttcga acatccttca tacctaagat gactgtgcag tcatattgcc agaaatgtaa
    48181 aaagatttgc tggtgttctt tagaatattc cgttaatata tctgttaatg caaaagggga
    48241 aaagtcgttt ttttgataaa tgtgctgatc tagtcgaatc gcagggttca ttcaaccggc
    48301 tcctttgtat aaattcatat tttctacaat attatactct aaaatcaaag agttgttttg
    48361 gaaaaaaggt tttctgtaaa aatggacaag ttggttgaca aaacaaaaga aactaggaac
    48421 aatgtagcta tgagaagtaa aggagaatga ataatggaat tatcacaagg aacaccgatt
    48481 tcaatcaaat tgcgtacaaa agtaaaacag caaggggaag tgcaagattt ttattttgat
    48541 ttgaaagggc aaatggtgac aattggcgat acgctgtaca tacgttataa agagattcag
    48601 gaggagacgg gcgatgagat tcctgtaacg atcaaattag ttccagatgg tcatgtacag
    48661 ctgatccgtg caggagaaat gcggatgcgt ttgaaatttg gctataaaga acggttagaa
    48721 acatcctatc gaacaccata cggtatgatc cagattgcta cttttacgaa agaactgcat
    48781 gttagtttga aagaccgtcc cacagcaggg aaggttcgaa tcgattatga tctgttcatg
    48841 ggaccggaaa aaatcggcga atattatttg accttggatt ttacagcgta aatataaaag
    48901 aaaagtttga ttttttgtgt ctgattttgt atgataagga gtagactgtg aaaggacgtg
    48961 tcctgattgg aaattagtgt atttgaaggt gcaaacaaaa gcgaattatc aatgatcgaa
    49021 gtagcacacg cgatcttaga acaacgtggc gatgtcatgg atttttctga tttagctaac
    49081 cagatccaaa actatcttgg gaaatcagac agtgaaattc gtgattcact cgcacaattt
    49141 tatactgact tgaatattga cggcagcttt atctcattag gggataatcg ttggggcttg
    49201 cgttcatggt atgctatcga ttcgatcgat gaagaagtaa accatggaat ggacgaagag
    49261 gatgaagata ctccacgtcg ccgtaaacgc aagaaagtca acgcctttat caatgatgat
    49321 gaagacgcga tcgattacaa tgacgatgac ccagaagata cagatctgac agaagaagat
    49381 gacgatgatc tgtttgatga cgatgatgat gaagatgaag aaatcgctgc ttataattct
    49441 gacttgcaag aaatcggagc agatgacccg acagatgatg aagaagactt gccaggaaat
    49501 atcgaagaag atttaagtat catagacgaa gacgatgatg atgaagatta tgaagaggaa
    49561 gaattttctg actctgaaaa agaataaaca ttattttctt acgagaggtt gtgacagaga
    49621 cgatcagcct atcagaataa ggaggtgtga cacgattttt gtcacacctt cttttttgta
    49681 tataaaaatc ccaagtaaat acataaaaaa taaaaaaatt gaaactttta gcgcaccttg
    49741 tacgtcatat taagtgaggc agaaaggagt ggagaatgat ttttactgga tataagaaaa
    49801 aacggcagat cgaaaaatac cgtcacctgc tgaatggact catcgaaact gattatcaaa
    49861 aaatgttccg tatcacgatg aattacgtac atgataaaga agaggcctta gatgtgatgc
    49921 aagatagttt tcataaagcc ctgaactatt ttgaaaaaga aaaagagatc gagcatttta
    49981 gtgcatggtt ttatcggata ttgatccgca cggcattaga tagttggcgc agacaaaaaa
    50041 ggacgcctgt ggacttattt gaaattcaag atatccccca acagttacct ttagaaactt
    50101 ccgttgcaga gctccatatg attttagata aaatagacag tccggaaaaa gagattctga
    50161 tcctctactt ttttgaagga tttcgtttga aagaaattgc gactatcttg gatatgaatg
    50221 aaaacacagt taaaacgaaa atgtaccgct cattgaatgc tttacgtcaa atcctagaat
    50281 gaaggagaga agagtgccat gtattcccaa aaagaaaatt taaaaaaact aaagaaaact
    50341 tataaagaac aagcaattcc caaagggctt aaagaggaga tccgccagcg ttttattatc
    50401 gaagagcaat catttataaa agcaaagcgg agaaagagac gtctccaagg gttcggagta
    50461 tctttggcta tcgtaatgat tacaacaagt agccttcttt ttaatagtca agttcgaagc
    50521 tttgcagaag atttacccat cttaggatca gtcattgaat tgatcttagg cgaacgcttt
    50581 actgatcgat ctgaaaaaat cgatattcag gtaccgaaga tcaatacaca aaatgagaaa
    50641 gaaaataaaa caattcatgg attaaatcaa aaatactttc gcgaaggaca agcagatttt
    50701 gaaaaagctg aaaaagaata tggaaatttt gaaacggatc attaccaagt tttgggagat
    50761 tatcagaaaa tcgtagacga taaccggttt ctcgtgattg aaaggcagat cacccaaaca
    50821 gcagcagata gccatgttga aaaaagatac gacacaatcg acaagaaaaa cagtgtccag
    50881 ctctcacttc cacttttatt caaagatgac acctatctat ctgttttgac aaaagaagtc
    50941 aaacggcaga tggccgaaca agtcaaggaa gatccatcta agtactattg gactgagcaa
    51001 gatattcaag aagggacgat agaaaaacca acacttgtta cgcctacacg aagtttttat
    51061 ttgaacaaag agcatcaatt agttttgact ttttctcaat acgaaattgc tcctggttat
    51121 atgggtacac cagaatttgt cattcctaag tccgtgacaa aaacgatttt ggcatcggaa
    51181 gattatttgg atcattagac gagaagtagt tgcgactagc agtttcaatt ttcaagtaag
    51241 tacaagtaaa taataagaag agaaaaagag aaagaagaga tatagtgaaa agaaattatt
    51301 tagttatcag tattattgct ttatccgtgc ttttgttttt cgaaattact tcggtggcct
    51361 ccttgatcac cgaagtaaaa ggacaaacac actcttcgga gcattcgtca caatctttca
    51421 aacaatcaga acaatcaacc attaaaagcc aatcaactga aacagctaac gtcaaagaag
    51481 ccattcaaga atcagcggaa aaggtacaaa gtcagtcatc acaatcaaca caagacagta
    51541 caaaaacgag ccgagatccg gctgtcaaaa gcgtcgcaat cagctttgat gatggaccag
    51601 gagcgaccac aacaccgcag cttctgcgaa tcttaaaaga aaaaaacgtg catgccactt
    51661 ttttcgtctt aggagaaaat acggcgcagc atcctgaaat cgtgaagcag acagctgaag
    51721 cagggcacga gattggcaat cacacatacg atcatcagaa tctggccact ctttctgccc
    51781 agtcgatgac ggaggaagtg acgaaagcag acactgagat caaaaaagtc actggcaaaa
    51841 cccccacttt tgttcgtccg ccctatggtt ctgtcacgaa tgtaggagca acgatcatcc
    51901 agcgaccgat catcgaatgg tctgttgatt cagaagattg gaagacaaga aatcctgatt
    51961 tgattttgca gaagatccaa gcaacagtat acgatggggc aattatttta ttccatgata
    52021 tctatcctga aacgattcgt gccgttcctc aagtgatcga ttatctgaaa gagcaagggt
    52081 atcgcatcac gacagttggg gatttattag ggcatccaac ggctgtcgag aactattatg
    52141 gcagaaacga tcatcggcca gttcagtaag tgagacaagt atctttaata aataacacat
    52201 tttaataagt agttgaacac ataactacct tacttgcaaa ggcttaacaa tatctataca
    52261 ggtacaacaa aaaactgtcc accaatttt
//
LOCUS       DAZUKC010000008        50608 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000008 DAZUKC010000000
VERSION     DAZUKC010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.008"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..730
                     /locus_tag="ACG80T_000446"
     CDS             <1..730
                     /locus_tag="ACG80T_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS4840128.1"
                     /translation="SILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEPKSIPTTLNV
                     ALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGMNKINAAYSY
                     GGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSLDLDGVYINK
                     GEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFKLPKAVGKLL
                     GSIQTNLPESVLLDCGMDFLKDIC"
     gene            788..2827
                     /locus_tag="ACG80T_000447"
     CDS             788..2827
                     /locus_tag="ACG80T_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFS4840129.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLLAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(2936..4087)
                     /locus_tag="ACG80T_000448"
     CDS             complement(2936..4087)
                     /locus_tag="ACG80T_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFS4840130.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4241..4771)
                     /locus_tag="ACG80T_000449"
     CDS             complement(4241..4771)
                     /locus_tag="ACG80T_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFS4840131.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(4870..5220)
                     /locus_tag="ACG80T_000450"
     CDS             complement(4870..5220)
                     /locus_tag="ACG80T_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFS4840132.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5377..6906)
                     /gene="ahpF"
                     /locus_tag="ACG80T_000451"
     CDS             complement(5377..6906)
                     /gene="ahpF"
                     /locus_tag="ACG80T_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFS4840133.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(6916..7479)
                     /gene="ahpC"
                     /locus_tag="ACG80T_000452"
     CDS             complement(6916..7479)
                     /gene="ahpC"
                     /locus_tag="ACG80T_000452"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFS4840134.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7623..8285)
                     /locus_tag="ACG80T_000453"
     CDS             complement(7623..8285)
                     /locus_tag="ACG80T_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4840135.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8469..10115
                     /locus_tag="ACG80T_000454"
     CDS             8469..10115
                     /locus_tag="ACG80T_000454"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFS4840136.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10106..11188
                     /gene="uxuA"
                     /locus_tag="ACG80T_000455"
     CDS             10106..11188
                     /gene="uxuA"
                     /locus_tag="ACG80T_000455"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS4840137.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11296..12849)
                     /locus_tag="ACG80T_000456"
     CDS             complement(11296..12849)
                     /locus_tag="ACG80T_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4840138.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            12997..14232
                     /locus_tag="ACG80T_000457"
     CDS             12997..14232
                     /locus_tag="ACG80T_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFS4840139.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKPEVAPEEKPDVTP
                     EPDTDSGNQTVPETNPDTDNETENPEKPEVDPEEKPDVTPEPDTDARDQGIPEKINKK
                     TIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAETLKSSKDTTFAVLKRKINS
                     YQNPESHKTKLYCGAESFY"
     gene            14205..14285
                     /locus_tag="ACG80T_000458"
     CDS             14205..14285
                     /locus_tag="ACG80T_000458"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840140.1"
                     /translation="MLWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(14411..15490)
                     /locus_tag="ACG80T_000459"
     CDS             complement(14411..15490)
                     /locus_tag="ACG80T_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS4840141.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(15593..16033)
                     /locus_tag="ACG80T_000460"
     CDS             complement(15593..16033)
                     /locus_tag="ACG80T_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS4840142.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(16090..16500)
                     /locus_tag="ACG80T_000461"
     CDS             complement(16090..16500)
                     /locus_tag="ACG80T_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFS4840143.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(16680..17114)
                     /locus_tag="ACG80T_000462"
     CDS             complement(16680..17114)
                     /locus_tag="ACG80T_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4840144.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(17214..18062)
                     /locus_tag="ACG80T_000463"
     CDS             complement(17214..18062)
                     /locus_tag="ACG80T_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4840145.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            18451..20217
                     /locus_tag="ACG80T_000464"
     CDS             18451..20217
                     /locus_tag="ACG80T_000464"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFS4840146.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            20319..21578
                     /locus_tag="ACG80T_000465"
     CDS             20319..21578
                     /locus_tag="ACG80T_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS4840147.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            21725..23026
                     /locus_tag="ACG80T_000466"
     CDS             21725..23026
                     /locus_tag="ACG80T_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4840148.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            23029..23880
                     /locus_tag="ACG80T_000467"
     CDS             23029..23880
                     /locus_tag="ACG80T_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFS4840149.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            23893..24708
                     /locus_tag="ACG80T_000468"
     CDS             23893..24708
                     /locus_tag="ACG80T_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFS4840150.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            24859..26025
                     /locus_tag="ACG80T_000469"
     CDS             24859..26025
                     /locus_tag="ACG80T_000469"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4840151.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            26255..26902
                     /locus_tag="ACG80T_000470"
     CDS             26255..26902
                     /locus_tag="ACG80T_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFS4840152.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            26986..27930
                     /locus_tag="ACG80T_000471"
     CDS             26986..27930
                     /locus_tag="ACG80T_000471"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFS4840153.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            28065..29516
                     /locus_tag="ACG80T_000472"
     CDS             28065..29516
                     /locus_tag="ACG80T_000472"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFS4840154.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            29537..30373
                     /locus_tag="ACG80T_000473"
     CDS             29537..30373
                     /locus_tag="ACG80T_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFS4840155.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            30373..31032
                     /locus_tag="ACG80T_000474"
     CDS             30373..31032
                     /locus_tag="ACG80T_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS4840156.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            31158..31847
                     /locus_tag="ACG80T_000475"
     CDS             31158..31847
                     /locus_tag="ACG80T_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840157.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            31853..32809
                     /locus_tag="ACG80T_000476"
     CDS             31853..32809
                     /locus_tag="ACG80T_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFS4840158.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            32853..33365
                     /locus_tag="ACG80T_000477"
     CDS             32853..33365
                     /locus_tag="ACG80T_000477"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4840159.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(33621..34169)
                     /locus_tag="ACG80T_000478"
     CDS             complement(33621..34169)
                     /locus_tag="ACG80T_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFS4840160.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(34258..35439)
                     /locus_tag="ACG80T_000479"
     CDS             complement(34258..35439)
                     /locus_tag="ACG80T_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFS4840161.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(35675..37183)
                     /locus_tag="ACG80T_000480"
     CDS             complement(35675..37183)
                     /locus_tag="ACG80T_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFS4840162.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            37342..37533
                     /locus_tag="ACG80T_000481"
     CDS             37342..37533
                     /locus_tag="ACG80T_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840163.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            37736..38653
                     /locus_tag="ACG80T_000482"
     CDS             37736..38653
                     /locus_tag="ACG80T_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFS4840164.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            38646..39719
                     /locus_tag="ACG80T_000483"
     CDS             38646..39719
                     /locus_tag="ACG80T_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840165.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            39867..40385
                     /locus_tag="ACG80T_000484"
     CDS             39867..40385
                     /locus_tag="ACG80T_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840166.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            40366..40758
                     /locus_tag="ACG80T_000485"
     CDS             40366..40758
                     /locus_tag="ACG80T_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFS4840167.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            40834..41769
                     /locus_tag="ACG80T_000486"
     CDS             40834..41769
                     /locus_tag="ACG80T_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS4840168.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            41902..42681
                     /locus_tag="ACG80T_000487"
     CDS             41902..42681
                     /locus_tag="ACG80T_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFS4840169.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            42674..42991
                     /locus_tag="ACG80T_000488"
     CDS             42674..42991
                     /locus_tag="ACG80T_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFS4840170.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            43235..44845
                     /locus_tag="ACG80T_000489"
     CDS             43235..44845
                     /locus_tag="ACG80T_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS4840171.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            45002..45850
                     /locus_tag="ACG80T_000490"
     CDS             45002..45850
                     /locus_tag="ACG80T_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS4840172.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            46081..46560
                     /locus_tag="ACG80T_000491"
     CDS             46081..46560
                     /locus_tag="ACG80T_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFS4840173.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            46593..48821
                     /locus_tag="ACG80T_000492"
     CDS             46593..48821
                     /locus_tag="ACG80T_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4840174.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            48950..50593
                     /locus_tag="ACG80T_000493"
     CDS             48950..50593
                     /locus_tag="ACG80T_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFS4840175.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
BASE COUNT        16932 a         9105 c         9815 g        14756 t
ORIGIN      
        1 atctattttg ttactgctta cattagtagt tggctatttt tcgattgagt ttatccatgg
       61 attttcgtct gcaaaacaga gttcaactgt gaaaaaggta gagccgaaaa gtatccctac
      121 cacactaaat gtggctttga ttggttcaga tgctcgttcg aaagacgaaa acggtcgctc
      181 ggattcactt ctggttgcac aatacaacca gaaaacacaa caagcaaaac tgatctctat
      241 catgagagat tcatatgtcg atattccagg ttacggaatg aataagataa atgcagctta
      301 ctcttatggg ggagttgatt tattaaatca aacattaaag gaaaatttca aatttgaagc
      361 tccctattat gcaagtatca catttcaaga ttttatcgat tgcgttaatg aactgtttcc
      421 tggtggagtc aagatcgatg cagaaaaatc tctagattta gatggcgtat atataaacaa
      481 aggagagcaa gtgatggatg gtaatacttt actccagtat gctcgatttc gggaagacaa
      541 agaaggagac ttcgggagga tcagaaggca gcagcaagtg atcaaagcta tctctcagca
      601 gctgaaagac gtaacttcaa tattcaagct acccaaagcg gttgggaaat tacttggtag
      661 tatccaaacg aaccttccag aaagtgtgct tctggattgt ggtatggatt tcctcaaaga
      721 catatgttag aataaacagg tataaatagt gaaataaagg aatgacaagc aagagaagga
      781 ggaaaaaatg aaaagaagtg acaagcacgg caaaaatcga acaggcgctt atattgccgg
      841 cgcagtgatc ttaatagcag ctgcgggtgg cggttatttt tactaccagc actatcaaga
      901 aacccaagca gtagaagctg gagaaaagac agttgagcaa tttgtccaag ctttgaacaa
      961 gggagattat aacaaagctg cagaaatgac atcaaaaaag gcggcaaata aaagtgcatt
     1021 atctgaaaaa gagatcttag ataaatacca gaatatatac ggtgctgctg atgtcaaagg
     1081 acttcagata tcgaatctaa aagtagataa aaaagacgat tctacttaca gtttttcata
     1141 taaagcaaag atgaatacct cattaggtga attgaaagat ctttcttata aaggaacatt
     1201 agacagaaat gatggtcaaa ccacgatcaa ctggcaaccc aacttggttt ttccagaaat
     1261 ggaaggaaat gacaaagtaa gcctgaccac acaagaagca gcaagaggga atattataga
     1321 tcgaaatggt gaaccattag caacaacagg caaactaaaa caattaggag tcgttccaag
     1381 taaacttgga gatggaggcg aaaaaacagc caatatcaaa gcgattgctt cctccttcga
     1441 cttaacagaa gatgctatca atcaggcgat ttcacaaagc tgggtacaac ccgattactt
     1501 tgtcccattg aaaatcattg atggagcaac gccagaactt ccagctggag ctaccattca
     1561 agaagtagac ggcagatatt atcctttggg tgaagcagct gctcagctga ttggttacgt
     1621 gggagatatc acagcagaag atattgataa gaatccagaa ttaagcagta atggaaaaat
     1681 cggtcgctca ggtttggaga tggcttttga taaggatctt cgtgggacaa caggtgggaa
     1741 attaagcatt acagatgcag acggtgtaga gaaaaaagtt ctgatcgaac atgaagttca
     1801 aaatggcaaa gatatcaaat tgacaatcga tgcgaaggca caaaaaacag cttttgacag
     1861 tctaggagga aaagctggat caactgttgc gacaacgcca aaaaccggtg atcttcttgc
     1921 gcttgctagc tctccaagct atgatccaaa caaaatgaca aacgggatct cacaagaaga
     1981 ttacaaagct tatgaagaaa atcctgaaca tccattcatc agccgatttg cgacaggtta
     2041 tgctcctgga tctacgttta aaataattac agcagcaatc ggtctcgaca acggcactat
     2101 cgatccaaat gaagtgttga cgatcaacgg gcttaaatgg caaaaagaca gttcttgggg
     2161 atcttttcaa acaacgcgtg tcagtagtga tgtatcacaa gtagacttaa aaactgcttt
     2221 gatctattcc gataatatat atgcggcaca agaaacgttg aaaatgggtg agaaaaaatt
     2281 tcgtacaggc ttagataaat tcatttttgg tgaagacctt gatttgccaa ttagtatgaa
     2341 tccagcacaa atttctaatg aagatagctt taactcagat atcttgctag ctgatactgg
     2401 atatggacag ggcgaacttc taattaatcc tatccagcaa gcagcaatgt attctgtttt
     2461 taccaacaat ggcacacttg tctatcctaa attgattgca gataaagaga caaaagataa
     2521 gaagaatgtc atcagcgaaa cagcagtaca aacgatcgtg ccagatctga gagaagttgt
     2581 gcaagatgta aatggtacag cacattctct ttctgcttta gggattccgt tggcagcgaa
     2641 aactggtaca gcggaaatca aagaaaaaca ggatgtaaag gggcaagaga acagtttctt
     2701 gcttgctttc aatcttgata accaaggtta tatgatggtc agcatgttgg aaaataaaga
     2761 agatgatgat tcagcaacca aacgagcatc ggaactattg caatacctca accaaaatta
     2821 tcaataaaaa attgcttttt aagtacagta aaaggactgt ggcgttatcg tcacagtcct
     2881 tttttcaaat aaacggtttt cacgatgtct ctttcacaac ttctttttga tttactcaca
     2941 cttcataaac tttttttgta aaatatttga gaacaagtgg agaaataagt gtacttaata
     3001 aaatcacgat aactaaaggt gaatagtact gtggttcgat caattgactt tgctgtccaa
     3061 tttgtaaaat gatcaaagcc atttctccac gcgaaatcat accagcaccg accatcaatg
     3121 aactatgttg agaaaaaccg gcaatctgag caccaaaata acctccaagc aatttggtca
     3181 aaatcgctac aagcgtcaat atagaaataa agagtagttg ttcactaaag tcagaaaaat
     3241 caacttctaa tcctacactg acaaaaaata ctggtataaa tactgcatat cctaaagcct
     3301 ctacattgtg gtatacttct tctttaacct ttgtctgact gacagctatc ccagcaaaaa
     3361 aagcaccaat gacagagcta agcccaacaa gatccgctac gtaagacatt cctaaacaaa
     3421 taactagcga tacgatgata atagaagaat ttgcgtacac tttttctgca aatgaaagta
     3481 ggaaaggcgc aatccatttg actaataaga aaataaaaac aaaataaatt agctgttcaa
     3541 tcaaaacaag cggtaaagaa gcattgctag aagcttcccc cgttagaaaa gataggctga
     3601 aacttagaac taaaacgact aaaatgtcat ctaccacaga agcacccaaa attgttgatc
     3661 cttcttttgt attcactacg tttagttctt ttaatacttc tactgaaata ctgacagaag
     3721 ttgccgcaag aataatccca aagaaaatcg cttcatttcc cgaaacttgg aatgctttgc
     3781 ctgtcaacca gccaaaaaca accgggaaga tgatcccaag caaagcaacg aacataccag
     3841 gtcggaaata ttttttcaaa agagataaat cactttccag accagctaaa aacattaata
     3901 agatcacacc aatctccgaa aagtcatgga ctagaatatc aggtttgacc caattcaatc
     3961 cagcttggcc tagcaaaaca ccaacaagta attgtccaat cactgctggg atacctaatc
     4021 tccgcgagat atgtgagcca atcgttgttg aaatcaatat caggcataaa ataccaataa
     4081 attccattct catcattcct ttgtataaaa tatagcccga acttagcata acatacatta
     4141 ttgaaaaaat aggaaaatat actcacaaaa aaaccaacaa tccaaaagat ttttttggat
     4201 tgttggtttt tatacggtct attttaataa ctaaaaatga ttaacgtata gtcatttttc
     4261 ttattctgat gaaattgata atctcctgga tgcccatgaa aatcaaaata aagccgaata
     4321 tttggaatag aagcatcaaa cttttgaatg gattgaacaa taagacgaat ccgccaatca
     4381 acaacagaat actactataa acactccaac ttttagcttt tgtattcatg ctgaagaaca
     4441 actggaacaa gctgttgatg ataacagcta atccaagaag aatcggcagg atagaaacga
     4501 ttgcaggtgc aaaagcatag acgatcagag ctaagatcag gaaaaaaaga ccactgaaca
     4561 gaccgatact ccaagaacct gttttccttt taaaacggta agcctcaaaa agattcaaca
     4621 acccaagcaa aacaagatag gcggtgatca gataaccgat aaaatgaaaa acagcagaag
     4681 ggtttagcac aatcgcaatc ccagaaataa tataaacagc agctcttaat aaggcgtatt
     4741 tttgaatatt ttgtttaatc gtctcaaaca tataagaaaa cctcctttga cttattttaa
     4801 cgtgaactag tcattttacc aaattaaatg ttacaaaatg agcgaaggag aagaatatct
     4861 ctggaaaaat caacttctga ggatcttttc catttttttg ccttttgcta gctcatcgat
     4921 cattttatct aaataacgaa tttctttcat caagttatcc tcgatgtctt ctactcgtac
     4981 accacaaaca acacctgtaa tcaaggaacg tgaaggattg gggacagggg cttccgcaaa
     5041 aaattgttcg aaagtcactt gtttttgtag ttgatattct agttctgttt gggaataacc
     5101 cgtcagccaa caaatgattt catctacttc ctcttttata cgatcttttc tctccgcttt
     5161 tgctacatac ataggataaa cgcttgcaaa acttgtcgta aaaatacgat tttgcgccat
     5221 aatcagtcac ctttcttttt ttcaaattat ttactactta tagtatataa gaaaaagaga
     5281 agaaaaagcg attattcaaa aaaacaaaaa ggattgtgac aaaattcttg tcaccatcct
     5341 tttttccgct ctctgacttt ttacaatctt catagctcaa gtgcgcatca gataatcgaa
     5401 tgctcctaaa gctgcagtag ctcctgaacc cattgaaaca atgatttgtt tgtatggact
     5461 atctgtacaa tcaccagctg cgaaaacgcc ggggatattc gttgcgccat ttttctcggt
     5521 cactatttca tttcttggat tcatttcgat cgttccatct agccaatccg tattagggag
     5581 taagccgact agaatgaata ctcctgaaac agctagtgta tgcatctcca tcgtacggcg
     5641 atcgatatag gagagagatt ctacctgatt cgtaccgctg atttctttcg tcgctgcatg
     5701 tttgataaca gtgacattcg caagagagac tagtttatcc tgtaagattt gatctgcttt
     5761 taattctggt aagaattcta gaacatatac atgttttgct aacccagcca aatcgattgc
     5821 tgcttcaaca ccagaattac caccaccgat aacagcaatc tcttttcctt taaataaagg
     5881 gccgtcacag tgtgggcaat aagcaattcc tttattttta aactcttttt cgcctggtac
     5941 attgatagaa cgccagtgcg cacctgtcgc gatgatggcg gtttttgctt taagtgatat
     6001 accgttagct aagccaatat taataaaatg ttgctcttta tttaaggaaa cggcctgttg
     6061 attcatcata atgtcgatag ggtaactacg cacatgactt tctacttgct gcatcaattt
     6121 tggaccttct gtgtaagagg taccaatcat attttcaatc cctagtgttt caacgacttg
     6181 tccgccgaaa gaatcagcga caagtcctgt acggattcct ttacgtgctg catagatagc
     6241 tgcactacta gctgctggtc caccgccaat tactaataca tcataaggtt ctttttcaaa
     6301 caactcttct tcaatcaaag ggccagtgat tttttcaaca atttgttcga tcgtcatgcg
     6361 tccgctacta aattcttctc catttaaaaa cacagttggc actgccatga tttttttatc
     6421 atcgatttct ttttggaaca tcccaccttc aatcatcgta tgagtaatat ttggatttaa
     6481 tacagctaat atattcaatg cctgtacaac gtcggggcaa ttatgacaag tcaagctaac
     6541 ataactgtca aaatgcaatg tttgtgttat ttttttgatt ttaagactca aagattcact
     6601 gattttcggt gcccgtccac taatttgtaa catagccagt aaaaaagagg aaaattcatg
     6661 accgaggggg ataccagcaa aaacgattcc actttctttg ccaactggat tgatagaaaa
     6721 actaggtgtg cgcatcgaat ctcttttttc ccaagagatt ttaggagaca tttctgctat
     6781 ttccttcgta aattcagcta gtttttgact attttcacta ttatccaaac taacagaaaa
     6841 aacaatagga gattcaatca gtgctaaata ttgttcgaga ttttgtttag tttctttgtc
     6901 taacatatcc accacctaaa tctttccaac aagatcaaaa cttggtttca atgtttcgcc
     6961 actttctttc cattttgccg gacaaacttc acctgggtgg gtacgtacat attgagcagc
     7021 gcgaatctta tcaatcagtg cactagcgtc acgaccgatt ccatctgcat tgatttccat
     7081 agcttgaacg attccgtctg gatcaataat aaatgttcct cgctgagcta atccttgctc
     7141 ttcatccaaa acatcaaaaa tacgagaaat ctgatgagac ggatcaccga tcatcacgta
     7201 ttcgatttta ccgatagcat ctgatgtatc atgccatgct ttgtgagtaa agtgggtatc
     7261 ggtagagcaa gaaaagactt caacacctaa agatttcaat gtactgtatt gttcttgtaa
     7321 gtcttctaat tcagtaggac aaacaaaagt aaaatcagct ggatagaagc aaagaatact
     7381 ccagttcccc ataagatctt tttcactgac ttctaaaaat tctccttgat gataagcatg
     7441 tgcattaaat tcttcgattt ttttcccaat caatgacata gtaaaacctc caataataat
     7501 tatttattta taattattgt aatttagttg gagttgcaaa gtcaatggat attttgttat
     7561 ttaataaaga ttactcaaaa acgaaggtta attataaaaa tagaagaggt cgcaagaaaa
     7621 tgctactgct ggaaatactc gggataacgt tgagtaacag tttcctgttc tttcagcatc
     7681 atgtgtaagt gcaatcccgt ttgaaatcga gcttcttctg gttttttatc aactaaagcc
     7741 ttgagtaaat cataatgttg ttccatgatc gtttcccatg gaagcccctg aatggtcact
     7801 cgtaaccagc ggaaacgttc taaatgggta ttatggtcat ctaaccagtt ccaaacttct
     7861 tttcttcctg caatttcgaa acaagcttca tgaaataaat tatctgcacg gaaaaaagcg
     7921 cgttcgtcat ttattttgac tgctttttct tgttcttcta aaatcgtttc tagcactatt
     7981 cgtgtttgtg tgtccaattt ggaacaacat tcaatcatga tttgctcttc caactgttct
     8041 cggacaaagc gagcattttc tgcagaaaca agattaattt tggaaacata cgtaccactt
     8101 tgaggtatcg tatacacgag ttcttgctga cgtagttgaa tcaatgcttc gcgaatggga
     8161 gtccggccga tagcaagatg ttcctccagt gccttttcag atattttcct tccaggttct
     8221 aagtcagcat agataatttg tttacgaatc gcttgataag cttgtgattg caagctgctt
     8281 ttcatcttta ttcctccaaa atcatttcta tgaacgtagt ataacagaag aggtctgaaa
     8341 aacaatctgg tatgtcacat taactagtaa atttgtataa atagtaaaat tttggttgac
     8401 atagcgattt ttattgtata aaattcactt gaatacaaaa actaatatgt tagtttagga
     8461 ggggaaaaat gattgattta aacaactgga ggaactttat ccctgaatta tctcaaaaag
     8521 aagtagaaat acctcatttt gaccgtgcat cattaagaga aaatggacaa aaaaatcctg
     8581 tctggatcca tttcggtggc ggaaatcttt atcgaggatt tcatgcagaa atcgctcaac
     8641 gtttagcgga tctaggcgaa ttgtccactg gtgtcattgt ttgtgaaaca ttcgatgaag
     8701 aaataattga aagggcttac caagattttg atgatttgat tcttgaagtg gtcatgccaa
     8761 gcagtggaga gttcgtcaaa cgtatattag ctgcgacagc tgacagtctg tattgtcatc
     8821 cagattgttc tgaacacttt gataagatga ttcgacactt tgaaaatcct agtttacaat
     8881 tagttacttt tgctattact gaaaaaggtt atcgattgaa agatacaaat aataaatata
     8941 taggaccgat tgcagtcgat ataaaaaatg gtccaaagaa gccatcacat acaatgagta
     9001 ttcttgctgc gctactttat aaacgttttt tatctggagc ttggccgatt gcacttgtct
     9061 ctacagataa tttttcacaa aacggtagtc aattacgtac ttctttgcta acgatcgcaa
     9121 aagaatggca aaaggaaggt tatgtacaaa atacatttat ccaatactta tcgaatgaag
     9181 aaattgttgc gtttccatgg acgatgattg atcgtatcac cccgaatcct tccttgtcag
     9241 ttagcaaaat gttggcaaaa gagggctttt caaatatgtc gattttccaa accgcaaaag
     9301 gaaataatat tgctccattt gtcaatacag aggacatcca ttatttaatc atggaagatc
     9361 attttcccaa tggacgtccg aaattcgaaa aagctggggt attcttgact gatcgtgcaa
     9421 cggtagataa agcagatacg atgaaggtga cgacttgcct taatcctttg catacgtctt
     9481 tagctattat gggttgttta ctggggtata ggagcatagc tgaggagatg aaagatccag
     9541 atttatcgct gctagtttct gaaatcgggt acaatgaagg cctcccagtt gtggagtctc
     9601 caggtatcat tgatccaaag atctttctgg aagaagtaat tactgtgcgc ttttctaatc
     9661 caaatattcc tgatatgcct cagcgaatcg ccacggatac ctctcaaaag gtggcgattc
     9721 gttatggtga gacgattaaa aaatatatgg aaatagaagt taaagacccg cagacacttg
     9781 tattcatacc attgactctc gctgcttggc ttcgatacct tattggcatt gatgatcaag
     9841 gaaaaccatt catcccgagt ccagacccac tattgacaga ctttcagcat aagctttccg
     9901 atattcaact gggtacgcaa gatacatcac atattcatga gaaaatggct cctattttgt
     9961 caaataaaga aatttttggt gtagatcttt accaagcaga gcttggcaga aaaatagaag
    10021 aaatgttcat caagatggta gcaggaccga atgccgtacg aaaaacatta caggattact
    10081 tacaagaaca tggaggaaat ttatcatgga aatgactttt agatggtacg gatcacagga
    10141 agaaaagatt accttagatg aaatcagaca gattcctgcg gtcaaaggga ttgtaggcac
    10201 attgatggat ataccagtag gagaggtatg gcccagggag aggatcaaag aactaaaaga
    10261 agaaattgag tctcgtgggt taacactaaa agtaattgaa agcgtcaatg tgcatgatgc
    10321 cattaaaatt ggattacctt cccgtgagca gtatatcgaa aactataagc agacaatccg
    10381 aaacttggca gaatatggaa tagaagtcat ctgctataat tttatgcctg tatttgactg
    10441 ggtgaaatct gaccttgact accgcttaga agacggctca tccacgttag cttttatatc
    10501 tgatgatatt ccagatgatc caagagaaat cgtcgcgcgc atcgaacatt ccagcaatga
    10561 atttgaattg ccgggatggg aacctgagcg cttagctcat atcaaatcct tgcttgaagc
    10621 ctatgcatca gtagacgaag aaaaacttcg agaaaatttt gcctattttc tccaatcaat
    10681 catcccaaca tgtgaagagg taggggtcaa aatggctgta catccagatg atccgccata
    10741 ctcgattttt ggattacctc gaatcgtaaa gaatcgagaa gatttagatt ggttgtgccg
    10801 catagtagac agcccttata acggaatcac tctttgtaca gggagtattg cagaagatcc
    10861 agaaaatgat gtatatggga ttttagcaga attttgtcaa agagatcgaa tcccatttgc
    10921 acatgttcga aatattaaat ttttagctaa tcgagatttc tatgaggcac cgcataaatc
    10981 cgaatacggt tcattagaca tgtaccgcat actaaaaaca atgtatgaca acggatttaa
    11041 cgaatacatc cgtcctgatc atggaagaat gatctggaat gagacaggaa gaccaggata
    11101 cggcttatat gatcgagcat taggtgctgt ttatctaaat ggattatggg aagcaatcga
    11161 aaaaggaaac aaaagtaaag ataattagtg aaaaaaactg taattttatt atgtaaataa
    11221 gaaggatgtt caaattcttg atacataaat ccggaggttg ttaataagca ttcggattta
    11281 tgtatcttat ttggtctagg aagtaacttg attttctttt ttcgattgat ccatgagtgc
    11341 ttcttctttt aattcaaaga tatattctga gatcctcata atcacttttt tccagtcttc
    11401 tggtgtgatg attcctttta aaatatagat ttttttgata tgagaaggca attgtttcaa
    11461 agggcagtcg ctgataacga tgttgcagtt ctccataaaa tcattttgta ttttgatcga
    11521 ttcatttgaa aaaatttgtt ctatcttaac gatcagatct ttactgatat aaaaatcacg
    11581 cgtatagttc acaccaatca caattcgcgg ctgttttttg tagcgatcat acaaatgtac
    11641 taggtcttca accatccatt gcacattttc cttttctaaa ataactggta acaagtgttg
    11701 attttgttct ctgaaatcca tataaaattg ttcaatggcg gtttggactt ctttcaacac
    11761 gctatgttct tgataaagca tgaaagtagg aaggggcgta tttggttgat tgaagttaag
    11821 aaatccatta taaagtttat ttcgtgcgaa tgccaatgag taaagaatct tttctttttc
    11881 aggtataaac aaatcaaata cagtacaaaa ggactccacc atttctcttg aataaagaac
    11941 atgaggaacg tttgcttttt ttaaaagttt gtattgctcc aagcttgttt cttccgattc
    12001 gattttcgtg atagaaagtc gagctaaaat attcactaag atgcgttgtt cgcgacaaat
    12061 tggcagatta ggtcgcaaaa tatctacaga gggatcaata aaaaagtcaa tatattcata
    12121 ctctgtgaat tctttttgta cgtctttcac agaagttgca ggaaaattct ctttacatat
    12181 ttttaataga aaatataatt tatctatcat accgctattt agtcggtcaa gtacttcttt
    12241 tttaaaaaaa gtattcatca taaaagccac tacttgcgga tcttcatcat aaaattctgt
    12301 agcagtactt gaaaagatgt tccaataaat atctaatgct gcatattgaa tttgtacctc
    12361 ttttccagaa aaagactttc tgccattatt agaaaaggag atagacaaac cgtacaatgc
    12421 taaaaatgca ttaatctttt tcattttcgt atacaaataa gaggcactat agtttgtttg
    12481 ctgacttaaa ctgataatcg aatttgtctg acagctgaac aaagaagtca agatctgata
    12541 actgactgat tcgttgcagt atcttccaaa tagacgataa tagcagtaag ccggatcaat
    12601 tccttcaaaa ttcaacttta aaaaaggttt ttgcttatca atatgcagtt ctgttagatt
    12661 gagacgctca aagtcttgtt ctaattcttt gatgtatagt aaaaaatttg attcagacat
    12721 atggaaatga tctgctaaat attgtgtagt aataaagaaa cgatgttcag ataggaaaag
    12781 aaaaatatct aattttgtat tgcttttttt cttcaagaga aacgttttaa tcgcttcttg
    12841 agtttgcatg aaagaggctc ctttttactt aaattgttaa aacttgaaaa tattactgtt
    12901 tttttcataa atttagacaa aaaaacagta aacatatagg tagaatctct actatcttat
    12961 ttcttgcata caaaaaaatc aaggagtgag agagttatga gatattcaac catcttaata
    13021 tcaacatttg cagccttaag cttatcgctt actataaatc agacagtcca cacagaggag
    13081 atagaaacag ccaaatggac agaaaccaag tgggccaacg tcaatcgaat tacctttcag
    13141 gatgaatttt catccttcaa ttcaggagtc gcaaaattaa gaaaaggaca aacagcgacc
    13201 atcaaagcaa aaattgacta ttcaggagac ttagcaccta tactacaatc agctgttaca
    13261 tttgagaatg ttgatcccgc tttgtcgatt ggctatgacg agaacacttt gactatccaa
    13321 cacaagcaag cgatatttac cttcaccgtt acattaaatc aggacttgtt aaaaccagcg
    13381 ctttttactg tcaaggtatc agatggacta ccgaacgatc atcatcattt aacgccgtat
    13441 agtcagcggc agacaattga gcaagattca gctatcgact caggaggaga tcttgttgaa
    13501 gaaccaacag ataaaccaga aaatgaaaat aagccagaag taccgcctac agaaaatcca
    13561 gacggagaac agaaaccgga aatagaacct ggagaagaac ccgatacaga aactcaacca
    13621 gagccagaca atgaatcaaa acctgaaata acccctggag aaaaaccaga tgtagaccca
    13681 gaagaaaaac cagatgtgac tccagaacca gatacggatt ctggaaacca aacagtaccg
    13741 gaaacaaatc cagacacaga taatgaaact gaaaatccag aaaaaccaga ggtagcccca
    13801 gaagaaaaac cagatgtaac tccagaacca gatacggatt ctggaaacca aacagtaccg
    13861 gaaacaaatc cagacacaga taatgaaact gaaaatccag aaaaaccaga ggtagatcca
    13921 gaagaaaaac cagatgtgac tccagaacca gatacggacg caagagacca aggaatacca
    13981 gaaaaaataa acaaaaaaac aattcaagag gatggaaaaa aagagagtaa aaaatcaaat
    14041 ctagcaattc taaaaataaa tgaagagcag ctgaataaaa aatcacgaat atttgatagc
    14101 gcccaatcag ctgaaacctt aaaaagtagc aaggatacaa cctttgctgt cctgaaacga
    14161 aaaataaaca gctaccaaaa tccggagagt cacaaaacaa agttatactg tggagcggaa
    14221 tcattctatt aagtatagca acaatgcttt ctgcaaaaag atttaaacaa aacagatctc
    14281 tctaacattt gaataatgtc tgtgaacgca tttttcaaat aaaaagaaat atccagaaaa
    14341 tgaccccaaa ataccagatt gaaatctgat atttggggtc gttcatactt tatctttctt
    14401 catatttatt ttaagggata tctgtttctt ttggaagatg gttcgggatc tttttcactt
    14461 tcaatacatc taaaaagaag gtaaagatcg gaatgccgac aatcaagccc caaacgccaa
    14521 agaaccgttc gctaacaagt aagaccacaa atgtataaaa aatcggcagt tctgttttgc
    14581 tggacatcaa cttcggattc agcacataag actcaattag atggacaatc aaaatcgtaa
    14641 tcaggatata aacaacatct cggatgcctc cttgggaata agctatgaaa gatagaggaa
    14701 tacaagaaat gataacacct gctactggga tcagacttaa aatgaaaatc atgatcgcta
    14761 aactcagcag ctgatgaaag ccaaatacag ccaaggcgat tgtagtgagc aacgtattta
    14821 aaagagcgac aacaaattga gcttctaata ccaaaccaaa tgtattgaca aacttatcgg
    14881 caaaatagta aatatcttga aagaaccaag aaaattcact ttttaaaaac aatcttgaga
    14941 atacaattgt ttttttcttt tcaatcataa aaaagaaact taagatgaat gacatagcaa
    15001 acgccactgc catttttcct atatcctgta aatagccaag cgcaatcgaa gcgccatttt
    15061 gaagctgttc aaaaaaatcg tttcgctcaa tataagtatg gatatattgt aaaacaggat
    15121 ctgcatcttt tggctgatgc tcgtaaaaat taataacgga ctggatcatg tcaaatgttt
    15181 gatgaataag catcgggata tactttgtga atgccagata gataaggtaa acgatcagtg
    15241 tataaatcat gatagctaaa acacctgtag ggattcggac aaatcgctga atgatttgaa
    15301 caagatgaat catcaaataa gtaaagataa atgtgagcaa aatggttgta atcatacttc
    15361 taactaagaa aagaacaaaa acgacaaata ataaaacagt gagacggcgt attttttcat
    15421 tcgccaaaaa acgctgataa acggtcaaat cgacaacctc caaataacaa aaaataaaca
    15481 cgttattcat tatatgatgg aatatgaaaa taagaaagag caaaaagaaa aaccgctaaa
    15541 tgacgttatc ttatgagttg gttttcctca taaaatcgtt catctagcag tttcagccct
    15601 ttttctggtc ttgatgaaaa ttatctagca tttttcgggt cagatgttgc agtgtatcca
    15661 attcttcctg ttcaagtgga agataagact tgatcttttg tggaatacct tctgcttgtt
    15721 tttgcaacgc agcaccttga gcagtcagcg taacatttac gattctttca tcacccgttt
    15781 cacgctgacg attaatataa tttttttctg ctaacttttt tagtaaggga gtcagcgtac
    15841 cagaatccag acacaaatca tttcctagct ctttcactga agtctttttc ttttcccata
    15901 aaactaacat cacaagatac tgggtatagg taagattcag cggcttgagg aggggagtgt
    15961 agtgtctaat gatttcttta gaggcggcat ataaaggaaa acatacttga ttaactaaat
    16021 atagatcttc catttttcca cctccgctgt tttactatag tgtatcatag cggatttaca
    16081 caaaaaagtc tattcactaa ctgctttcag agtaacttct atatttccag ctgtagcttt
    16141 tgaatacgga cacactttat gcgctgcctc taataattct tgcgtttctt ccagagaaag
    16201 accttcgata gatccttcaa tctctacacc taagacaaag ctcgtttcac tttctgaata
    16261 aagggaaact tgtgcactga cagtagaagc cccagagata tttttttgct gtttgatcaa
    16321 ttctaatgca ctgttaaaac atgcactgaa accagctgca aacaattgtt cagggttggt
    16381 tgcattttcg acgcgtttac caggagctgc aatattcaat tcgaatgtac gatcgggatt
    16441 atgcacttct ccagagcgtc cgcctgtgtt gatcattttc gtagaataca tttttttcat
    16501 aaaattacca cctttaatta tattttatac aattgaattt tacacaatat aattgtggaa
    16561 agcaaagaaa tggattggca taagataaat aagagaaagc acatgaaaca gacgcgcttt
    16621 ctcgaatagc aaacatatac aaaaaatatc agagacaaaa aataatctca tcataaacat
    16681 tacatatatt tctcaaaaag ttgttttctt tcttcaaaaa ttacttcacg atggacctga
    16741 tgaagcatcc ataaataacc aaaaggatct ctgaacattg agttagtcac accaaagtcc
    16801 atcatttctg tcatttcttg aatctctaca cagccatttc gtaatgcttt attatgtgtg
    16861 tcctcgatat cagagacgat gatattgaac cagatcgtat ttggatgttc agcagtagga
    16921 gcagtcaaac caagagacgt gttttcatcc agcatatgga atctcgtacc atacaaggta
    16981 aaaatgactt cattattgcc tctagggtaa tctgttgcct caatcagttg tatttcatca
    17041 aagattgttt gatacagttc tagtgctttc aaactatcat ttaatacaaa atcgatttcc
    17101 gttcctttat gcataaagat ctcctctcct tttcagtcga acagttacta gattctttta
    17161 ttataccagc taaagaaaac gcattcaaaa aaatagcgga tttacttacc aactcattcg
    17221 attggatgat aagtaaatcc gcgtcccatt acttctgcta cgctgctgac aatgacaaag
    17281 gcacttggat cgatttcttc tatcaatggt ttcattcggg aaaattcatg atcagtcaca
    17341 acgatcaata aaacctcttt tgactttcga ttatacccgc cttttgcctc tagcagggta
    17401 aggccacgtc caatctcagc acttaagcga aggcggatgt cttccaaata atgttcactc
    17461 atgaccataa cagatttctt tcgattgata cctgtttcga tataagtcat gacaattgaa
    17521 gtgattgcaa tagataaaat agcataaaga aactcttcta ttccaaaaat aaacagatta
    17581 agagaaacaa cgaatgcgtc tgtaaaaaat aagccaatcg aagtattcaa gtggaaatat
    17641 tttttaaaaa tgaggggagg gatcgtcgta ccgccgctag aagagttgtt tttatataaa
    17701 atcgcaactc ctaaagcaaa aatcgcactt ccaaaaatga cagacaacaa ccgatctgaa
    17761 gtaatcattg tttctggaac tattgcaatg attaaaggaa aaaccaagct tccgaaaagt
    17821 actttaaaaa agggtttttt acctaaaaat agaagagcta gtaccaacat aacgatattc
    17881 aacactaaaa taactatagc tcggtcaaag cctaaagcag cttccagtaa tattcctaga
    17941 ccgctgacac ctccagcagc aatatgatga ggccctaaaa acatattgat agcgactcct
    18001 aatatgaaga gggcaaaaat aatcttaatg tatgtccata taaattgtct cgtttctttc
    18061 atttattcac ttccttaatt cattaataat actcttatct tattataact tacttttcac
    18121 cttaagttaa ctttaattaa taaaaaaatt aggctagtct aaattattat gtttcattct
    18181 tctagaatat acttctacgc caaaaactgt taaaaaactt ttactttaga aaattggaga
    18241 aattgatcgc ttaagataaa ttcaggacac taaatagtac aggaagtaga ctaaaacttt
    18301 taaaagtttt aagtctatct atttggcaag tttaaaaaat aaaaattcgg tatcaaaaaa
    18361 ctgttaccga taacattttt gaaagcgttg tcttttgctt ctctatcact tatagtgatg
    18421 acgtcatatt aaaacacaag gaggaaatca atggatacag ctgcaattta tcatcgaccg
    18481 gaaagtgaat atgcatttct atatacgaat acaagatttc gtattcggct tcgtactagg
    18541 aaaaatgata tcaaaaaagt atatgtgcta tgtggagatc catataccat aacaactgaa
    18601 aaatggtatc aaaaacaacg accaatgaaa aaatgtctaa gtaccaatgt tcatgattat
    18661 tgggaaattg aagtgacgag cgaaacaagt cgcttacagt atgcgttcca tgttgttggg
    18721 gaagataaca cggactgttt ttacggtgat caaggaattt ttccttatcg agaaaatgtg
    18781 attactgaac caaattttta ttttcgaata ccttattttc atcaaatcga tcgattcact
    18841 attccagaat gggtaaaaga aacagtatgg tatcaaattt ttcccgaaag atttgcaaac
    18901 ggggacaaag caaatgaccc cgaacacact ttaccttggg gaagtaaaga tccagaccga
    18961 gaagactttt tcgggggaga tttgcaaggt gtgatggatc atttggatta cttgacggac
    19021 cttggcatca atggcatcta tttttgtccc atatttaaag ccacatccaa tcataaatac
    19081 gatacgattg attattatga ggtggatccc gcttttggcg ataaaaaact tttaaaaaac
    19141 ttgattgatg aagcacataa aagagggata cgtatcatgt tcgatgctgt attcaatcat
    19201 atgggtgtcc attcaccaca gtggcaagac gttctcaaaa acggagaaaa atcgatctat
    19261 aaagactggt tccacatccg ttttttccca gtagattctt atcagatgac tgacttacca
    19321 gaaactgctg aaaatatccc atatgatact tttgctttta ctccattcat gccgaaacta
    19381 aatacagcaa atccagaagt gcaaaaatat ttgctggata tcgcaactta ttggataaaa
    19441 gaattcgata ttgatggatg gcgtcttgat gttgctaatg aagtcgatca tcatttttgg
    19501 aaaaaattca gagaagcagt tacagaaatc aaaccagaca tctatatcct tggagagatt
    19561 tggcattcct ctcaagcttg gctgcaaggg gatgaattcc atgcagtgat gaattatgca
    19621 tttacagatt cgattaaaga ttattttgca aaaaagaaaa tcacagcttc acaaatggtc
    19681 agtggaatga atcatcagca aatgctttac agagatcagg tgaacgaagg aacattcaac
    19741 ttattagatt ctcatgatac tgccaggatc ttgacccttt gccaaggtaa caaagaattg
    19801 atgaagtctg tgatggcctt tatgtttctt caaaaagggt ctccttgtat ctattacggg
    19861 acagaaatag ggatgactgg tgaagatgac ccggattgcc gcaaatgtat gatatgggag
    19921 aaggaggaac aagatctaga gctattcggt tttataaaag aacttgtttc tctaaggaaa
    19981 cagttgtcta agattatttc tgaaggttct actcagtggc taatagtaaa tgatcgtgaa
    20041 gataagctct atttcacacg gaaactagag ggacagatca tctatgttta ctttaatcag
    20101 tcaaaagaac cttgggtagt agaacaggaa aatgaggtga ttcttagtca aaattgtcag
    20161 ttgttagaag acgggaaagc agagatacga caatacggat ttttgatcat gtgttgaaaa
    20221 ctgttaccgg taacaacaag aaagcgtttg caaatgaaga tttgtgccca ttataataag
    20281 ggtgtaacag cacgttgaat acaaaggagg aaatgaagat gaaaaaaaga agtgtgaaac
    20341 agttgggttt agggttaatc tctttaggaa tcttaggcgg attagcggct tgtggcaatg
    20401 gtaactcttc agcagaaggg gaagccgatg aaggaaaaac gttaacagtt tccgttgatt
    20461 ctggatataa agattatata aacgaaataa aaaacacttt cgaaaaagaa aacgatgtga
    20521 aaatcaagct agtagaaaag gacatgttcg atcagttgga atcattagcg ttagatgggc
    20581 ctgctggaaa gggacctgac gttatgatgg cagcatatga ccggatcggt gcgttaggac
    20641 agcaaggaca tctggcggaa gtaaaactgg gaaatgaagc agcttacgat gaaacagaca
    20701 aagcccaagt gacttatgat ggaaaaatct acggagaacc tgcagtaatc gagacccttg
    20761 ttttgtacta taacaaagat ttagtcgata cagcaccatc aacgtttaaa gatttggaga
    20821 atctatccaa agacagtcgt tttgcttttg agtcagaagc aggaaaaaat acaggatttt
    20881 tagctaaatg gactgacttt tattattcct atggtttaat tgcaggttac ggtggctatg
    20941 tatttggtga tgatggcaca aatccttcag atatcggttt gaataatgca ggtgctgtag
    21001 aaggaatctc ttatgcaaca gattggttca aaaatgtttg gccaaaggga atgcaagaca
    21061 taaaaagtgc aggagacttc gccagccagc aattcatgtc caataaaact gcagcaatca
    21121 ttgacggtcc atggcaagcg caaacataca aagaaaacaa tatcaattat ggtgtagcca
    21181 aaattcctac tctaaataat ggacagcctt atcaaccgtt tggcggagga aaaggatggg
    21241 tagtcagcaa ttatgctaaa aacaaagacc tctctcaaaa atggcttgat tatgtaacga
    21301 atcaggaaaa tcaagaaaaa ttctttgaaa tggtaaatga aatccctgca aaccaacaag
    21361 ctagggaaac agcaaaggct aaaaacgatg aattaacaac agctgtgatt gaacaatatg
    21421 aaacagctca agcgatgcca aacatcccag aaatgggcga agtttggaca ggtgcagaaa
    21481 acttgatgtt cgatgcagca tcaggttcaa aaacaccaaa agaatccgca gatgaagcag
    21541 taaaaacaat cagtgaagcc atagaacaaa aatactgatt aaaatgataa ccgtccattt
    21601 ttataaaaaa tggaacaggt gcttcagata aaagtggcaa gagcgagatg gaaaattatc
    21661 ttgctcttgt tgccattatc ttaaaagcag tttgcggttt agcaagttta gagggggcat
    21721 tcagatgcag caagataaaa caagcaatat caacgaaaaa aaaatcacgc tttgttcact
    21781 tattccaggg atggggcagt ttgtaaatgg ccaagctttt aaaggtatcg tttttttcgc
    21841 catattcgtc ctattcattg gtcagatgct tctgggcgga tttcatgcat tggtaggatt
    21901 ggttactttg ggaagtatac caatggaaga tcattcatta tttattttga ttgaaggcac
    21961 tttgcaattc atcattacaa tcattttctt gattttttac ggtttgaatc ttttagacgc
    22021 ttatcgagta gcgaagatgc gtcaagaggg caaaaaaatc aatcggacga tgaaggaagt
    22081 catacataat ataggtgatg aaggttttcc ttatctattg acacttcccg cttatcttct
    22141 catgattttt acgatcattt tcccagtcct agttacattg ttcactgcat ttactaatta
    22201 tgacttcaat catattccac cagccagttt aattgattgg gtaggggcta aaaacttttt
    22261 tagtatcttc tttttaagct catatcgtaa tacttttttt gcagtctttt cgtggaccgt
    22321 gatttggact ttatgtgcaa caactttgca aatcaccctt ggtattctca cagcggttgt
    22381 tgctcaccag aaatttatta aattcaaacg tattttcggt atcattttct tattaccatg
    22441 ggcggtccca gcttttatta ctatcatgag tttttcgaat atattcaacg atagcgtagg
    22501 tgccatcaat actcaagtga ttcctttcat aaaccagctt ccttttgtag atatcagtgc
    22561 aatctcttgg aaaacggacc cgaactggac gaaagtagca attatcttag tgcagggctg
    22621 gttaggattt ccctatatct atgtgatggt tacaggaatc ttacaatcga tttcagaaga
    22681 cttatatgaa gcagcaaaaa tcgacggagc aaatgcatgg cagcgctttt caaacatcac
    22741 gcttcctgct gtcttcctag tggcagcgcc aacatttatt acacagtata cggggaattt
    22801 caataatttc tccatgatct atctgtttaa caatggcggt cccggaagcg taggcggagg
    22861 agctggttct actgatattc ttatttcctg gatctataaa cttacgactg gaacttctcc
    22921 gcagtattct atggcttcag caatcacgtt gattatctcc gtcatcgtta tctgtgtgtc
    22981 attgctggta tttaaaaaga caaatgcgtt caatatgggg gattaacaat gaaaaaattt
    23041 cattcgagtg ctcgattcca aaaacgactt tctcactttt ttacttactt ttttctttta
    23101 atactgtcag tcattatcat ctatccgctt ttgatcacag caagtactgc cttcaagtca
    23161 ggaaatattg cagcttttag tttagatctt tcaagtgact ggactttagg aaactttact
    23221 cggctattca gtgaaacact ttacgggact tggtataaaa atacattgat catttctgtc
    23281 ctaacaatga tcgtacaagt gactgtagta actttggcgg gctatactta tagtcgctat
    23341 cgttttatcg gccgcaaatc aagtttgatg ttctttttag tgattcagat ggtaccgact
    23401 atggcagcat tgactgcctt ttatgtcatg gctttcttat tagaagcgct cgataagtac
    23461 tggttcttga caatgatcta tataggcggt ggaattccaa tgaatacttg gctgatgaaa
    23521 ggatactttg acactgtacc aatagattta gatgaatctg ctaaacttga tggggcaggg
    23581 catttcagga tttttttcca gattgtatta ccgcttgtca aaccgatgct cgccgttcaa
    23641 gcattatggg cttttatggg accttttggg gatttcatgt tagctaagtt cctgcttcga
    23701 acacctgaaa atcagactgt tgctgtcggc ttacaaacat ttatttctga tgtgacaaac
    23761 caaagagtct ctctttttgc tgctggggca attttgattg cactaccgat ttgtctatta
    23821 ttcttctttt tacaaaagaa ctttgttacc ggcctgacat ctggcggaac aaaaggatag
    23881 gagcagagta tcatgcaaac atttccgtta aactattttt ccgcattacg tacgccaact
    23941 caagtgtttg cgggacgcaa actattatca tggcctaaat ttttcttgat atttgtattt
    24001 ttagtttcct tgatggtcat gcctgtgact cttttttatg ccaatcagat ccaagcgatt
    24061 ccattagaac aatttctatc cgttcattcg ctgatcgatg aacaaggaac ccaaaaattt
    24121 tctgaacttg aattatctga aactgggtta caagcatcgc aacaaacgat agctgtgacc
    24181 cctgaaattt tggtaggtgt gtctttatct gagaaacaac aatccgatca tggaactttt
    24241 attgattttg agaaggaaca atgggtgatc caacaaaagg ataaatctgg tatacgccgc
    24301 tatacgatga attatagccc atctttccaa ccagattctg tacgtacgcc cgaagatttc
    24361 caacgatttt tagaaagaga attttatgcc agtaatcgtc caacaatcat tttatcttat
    24421 agtttaagtc ttggtcttgt cctatttgtc atgacgagtc tcattttatt cggggcttcc
    24481 tttttcttgt ggatgacaag aaaaagccaa ctttcatcca ttcatacatt caaagagagt
    24541 gcaaatttga tgctgaatgt tatgggaata ggttccatgg cagcagcagt tgtagggttt
    24601 atccactttg actttatctt gatgctaagt gtccaaacaa cgataactgt cttgctgctt
    24661 ttatgggttt ttgtcaaaac aagattcaaa gataagcgtg tcgaatgaca agtttaagcg
    24721 atttatgcga caatgaatgt gaaaaaaatg aaaaataaag tagtttttga aaaatttctg
    24781 aaaaaattaa acttcttagt tgattctttt cttttttagt attaacgtaa tagggtagtt
    24841 tttttaagga ggaaaatcat gttagagaaa actttataca atcaattatt ttcacgttct
    24901 ttctcgctcc ctgtcgaagt aaattattgg gacggtacaa cgaaacaata cggagatacg
    24961 gacactcctc cccaaatcaa aatcaatatc catgaagaaa tcccaatgaa agagattaca
    25021 agtaacgctt ctttggcact aggagaagcc tatatggatc atcgaatcga gatcgaggga
    25081 agtatccaag cattgatcaa cgatgcttat cagcaagcaa acagcttttt acgaggaaaa
    25141 gattatctaa aatggctgcc aaagaaagaa aaacatacga aacaaaaaaa caaagaagat
    25201 atccatgcgc attatgactt aggtaacgac ttttataaac tttggctaga tccaactttg
    25261 acttattctt gtgcttattt caatacacct gaagatacgt tggaacaagc acaaatcaat
    25321 aaagtccatc atatcttaga caaactcttt atcaaagaag gcgaaacgtt gcttgatatc
    25381 ggttgtggct ggggaacatt gatgttcact gctaccaaag agtataatgt caaggcaact
    25441 gggatcacac taagtgaaga acaatacgat ttgattacca aaagaataaa agaagaacat
    25501 ttagaagata aatgccgtgt attattaatg gattaccgcg aactaaaagg cgaaacgttt
    25561 gatcatatca caagtgtagg tatgttcgaa catgtaggtt ctgaaaatct tgaaggttat
    25621 ttcaaagtgg taaaagattt attgaaacca aaaggaacag ctttgatcca tggaatcagc
    25681 cgacaacaag gcggtgcgac aaatgcttgg atcaataaat atattttccc tggcggttat
    25741 gtccctggtg ttactgaatt agttgataat attacaaaaa acgatttgca actgattgat
    25801 ttggaaagct tacgtcgtga ctaccaattg acattagaac attggacaaa gaattttcat
    25861 caagtccaag atcaagtcac gaaagaaatg ggtgattcct tctatcgaat gtgggatcta
    25921 tatttacaag cttgtgcagc ttcatttgaa tcaagtaata tcgatgtgat tcaatatttg
    25981 cttgttcaac ctggaaacaa tgacatccca atgcatcgtg cataattgag gcaaaagtac
    26041 tgtttaaaga ctatttaaaa agaagctaag ttttatgatg ctccagaagt gaactttttt
    26101 gtctaacttc taggattgtc tctaaagctt agtttctttt tctttatatt ttcataataa
    26161 aagtttggtt ctttaatttt taaacatgac agaaaatttt ttcttcgtta tacttgtaat
    26221 agaatactat tttataacaa aggggatcga atcaatgaaa aaaactgtta tttatactag
    26281 tttatttgca gctttgtttt tagctgcttg tcaatcaaat accaatcaag gcacatctgg
    26341 gaatacgtct tccagtagcg aacagcgctc ttcacaggta tcggactcct caacaaaagc
    26401 aagcacatct tcgtctagcc agacaaaaag ttctagtgaa gcaagtactc agtcagatat
    26461 caaagtgtct gcagctcgtg cgatcgaatt gttccaagat aaatacccag atactgcaat
    26521 tactagtttg gaattagata gtgattgggg atcttacttc tacaagatag aaggtgtcga
    26581 tgatcaaaaa gaatacgaag caagaattga tgctcaaaat gaaaaaatgg agacagagaa
    26641 tccagaaact ctagatcgag atgaacaaaa tggtcaaaag aaaaaagaag atggtctgga
    26701 tgttgataaa ttgatcacga ttgaggaagc agcaaaaatc gccgtcgaca aagtaggaag
    26761 cggtacggca acagattggg atttggataa agatttaggt actacttatt gggaagtcaa
    26821 agtaaaagac ggcaatcaaa gtacatcagt caagatcaat agccaaacag gcgaagtact
    26881 gtcaactgaa caagatgatt aagaatttct tcggaaaaca agagttgatt tttcttttag
    26941 tcatgataca ctagacaaaa taacttgttt gaaggagcga tttacttgag tttagaaacg
    27001 acctttgcca atcatatctt tgccaatcct ttgatgaatg catctggtgt ccactgcatg
    27061 actacccagg aattagatga actggctcat tcagaagcag gagcatttat cactaagagc
    27121 tgtacgatca atgaacgaaa aggaaatccc gaaccacgct attttgatgt tcctttaggg
    27181 agtatcaatt ctatgggact tccaaactta ggtttttctt attatctaga gtatgctctt
    27241 gcgtatgaaa aagtgcagga aaaccaaaat caaccgttat ttttttccat tgccgggatg
    27301 agtgtccagg aaaacttgga aatgcttgag aaaatagaaa aaagtgggtt taatgggatc
    27361 actgaattaa atttatcttg tcccaatgtg cctggaaaac cacaactagc ttacgatttt
    27421 gaggccactt atgaaacact gaaagaagta ttcagcatct tttctaagcc gctaggaatc
    27481 aaattgcctc cttattttga ttttgctcat tttgatcaga tggctgatat cttgaatcag
    27541 tttccattga cctatgtcaa tgctatcaac agtgtgggca atggtttgta tatcgataca
    27601 gaacaagaag cagtagtcat caaaccaaaa gaagggttcg gcggcattgg cggagagtat
    27661 atcaagccga ctgctttggc gaatgttcgc gctttttata cacgtttgaa accagaaatc
    27721 caaatcattg gtacaggtgg gattcggaca ggacaagatg catttgaaca tctgctatgt
    27781 ggcgcaagca tgttacagat tggtaccgag cttcataaag aagggccgga aattttttct
    27841 agaatcataa aagaattaac gcagattatg tcagaaaaag gatatacgtc aattgacgaa
    27901 tttaaaggca aattacgcac catttcatag caaagagacg aaggtcatga ttgatcttcg
    27961 tcttttttta taaaaaacac tcgtctgttc attcctgtgc aagcgtactc attgtatgtt
    28021 agacgaactt gtgggaaaat aacagaggca aaaaggagga taatatgaat gatgcaacag
    28081 aattagcaaa agagcttaaa gaaaaagaat ttagttttca agaactagtt acggatatac
    28141 agaaaaaaat aaagaagcaa aatcatgaat tgaatgcgtt tgtcgcgctt gaaccattcg
    28201 agagtaattc ccacgagcta ttgattcccc agtcagaaaa agggccattg gctggtatcc
    28261 catttccttt aaaaatgctc ggtcaagaaa aaaagggatg gctagctact tcaggatctc
    28321 gtttatttga aacacaccga gcttctcata catctaatta tgtgcggcaa gcagaagcga
    28381 taggtctagt tccttttgga caaaccaatg cgccagagtt tggtttcaaa aatattacag
    28441 acccagtcat ctatggccca gcaagaaacc cttggaacct tgaatgtaca ccgggcggat
    28501 caagtggagg agctgctgct gcagtttcat caggaatcgt tcctttagct ggtgctagtg
    28561 atggcggtgg ttctattaga atacccgctt cgttttgcgg attgattggg ctgaaacctt
    28621 cgcgagggac gatgccagtt ggtccatatg cttggagagg atggcaagga gctgctattg
    28681 atttcggctt gacagtttct atgagagaca cggaggcttt attttatggg atgagaagta
    28741 tccattccgg tgcgccatat caagtatcgc cagttgagtg gcaaacacat ccaagaaaaa
    28801 aacggttgaa gatagctttt tgtgttgatt caccgatcca ctcacagatt agtgaagagg
    28861 cagtacttgc tgtaaaaaaa gctgtagatt tccttggaaa agaagggcat gatttgacag
    28921 agattcctta cccactagat ggtcgtaaac tgatcacgtc ttattatcag atgaatggcg
    28981 cagaaacagc agcaatgatc cgctcgattg aaacaggcat ccagcgagct gtcagaaaag
    29041 aagaacttga acccatttct tggactttgt tccaatatgg tcagaagctc tctgccgcta
    29101 cttacatcga ttctctgcat gtatgggatg aagcagcaat tgtcatggaa aacctacatc
    29161 aagaatacga tctattcctt tcaccaacga ctgcatttac cgcccctaaa atcagcgaag
    29221 atttgcaaag tgatgcgata agagcacaga tggaacacat cagtgattgt tctgaagtag
    29281 aaggtttaga cgttgtttct tccatgtttg aggagagctt gaagatcaca ccttatacac
    29341 agttggctaa tctgactgga caaccagcaa tcagcttgcc tactcatttg acaaaagaaa
    29401 acatgcctct aggaatccaa tttatggctg ccagaggaag agaagatttg ctatttcaaa
    29461 tcggctatct atttgaagca aatcatcagt tccatttacc aaatccatgg aaatagcaga
    29521 aacaaggaga agaagaatga agaaaaaaag attgtttgct ctaataacta gtgccttttt
    29581 tattttaagc gcttgcggta cagctgccgc taatcaggaa gataacagct ggagtaatgt
    29641 agaaaaaaag aaagaattag tagtagcgac ttctggcaca ctgtttcccg catcttatta
    29701 taatgatcaa aatgaattag ttggttatga tgtagatttg gcaaaagagg tggctaaacg
    29761 gttaaatgtc aaaatctcct ttaaagaata caacgtggat ggacaaatca cttctgtttc
    29821 aaaaagagaa tcagattttg ctgcgaacga ttttggtatc acaaaagaaa gagaaaaaaa
    29881 attctccctt tcagagactt tcaaatattc atttgacagt atgatcgtac gaaaaaaaga
    29941 tgattcagga atccactcac ttgaagatct taaagggaaa aaagcagctg gcgaacccaa
    30001 tactagctat atgcgtttag cggaaaaaca cggagctaaa ttagtcacat atgacaatgc
    30061 cacgaatgat cagtatttaa gcgatgtagc aaatggtcga acagatgtta ttctaaatga
    30121 ttattatctg cagaaaatgg ccgtatctgc tttaccagat atccctgtaa aaatactgga
    30181 gaacgtttat ttcaatgaaa acgaaagtgg ttttttattg aaagaaggaa atgatgctct
    30241 gaaaaagaaa atcgatggcg tattaaaaga gatgaaagaa gatgggacga caaagaaaat
    30301 atctgaaaaa tattttcaaa ctgatgtttc agtcaaaccg aaagaaacga tggctgagtc
    30361 ggtgagtgag tgatgtactt tcctctaatt gatttttccc tcatgtggac ttcacttcct
    30421 ttcgtactgc aaggcttggt atacacatta gggattggct tcgtgtcgtt tcttttagga
    30481 aatctggtag ggttgctttt gacagtttta ggtcttttgg attggctgcc tctgaatgta
    30541 tttattcgtt tttatttatc gttctttaga ggcattcccg cactagtcct gctgtttctg
    30601 ttatattttg ggttacctta tcaattaagt gccttaacgg cttcagtcat ctgctttacg
    30661 attaccagca gtgcatttat tggtgaaatc tatcgaggat cactagcagg cgtttcttca
    30721 ggtcagtggg acgctggttt agcttctggt ttttctttct ttggggtatt gaaatatatc
    30781 gttttgccgc aagcttttcg aatctcagta ccggcactca gtaacgtagc tatggatcta
    30841 gtgaaaggta cttcgttagc agcaacaatc acagtgccag aaatatttca aaaagcaaag
    30901 atcataggcg gtcggacatt cgattatttt tctctctata ttttagttgc actgatttat
    30961 tgggtcattt gtatcgggat cgaaaaagtg caacagtatt tagaaaaacg ttctatgcat
    31021 cagtcaatct gaaaaataga aatggtgcta gcgtggctgg caccatttct attttttgtt
    31081 tcttgtatat tctgcctagt attttaaaac catttcatgt aaaatgtaaa tcgaaaatat
    31141 gttcggaggt agaaaacatg aagaccccgc taaccatcga aatagaatcc tcgttatacc
    31201 aatattgcat cgagcaaggc ggcttggttg tagaagaagt cacgatgcct gatgaccaag
    31261 gaattgtaga tacgcttagc tgcttgacaa aatttgatgg aagccgagaa tggcgatgct
    31321 atgagctgaa agtgacaaaa gcagattttc gctctacagc caaactgtca tttgttggac
    31381 attataatta ttttgtttta ccaagtaaac tatatgaaga agtaaaaact gaaatcccta
    31441 caggaatagg tgttttagtg tatcgtccgt acacggttga agaggagatg cctgcctctg
    31501 gtacatttct gattgcgaaa aaagcagcaa agaaagagct tgctgtaccg gaagaagctt
    31561 tgacaaaccg cttcatggct tctttattca gggaagtaag aaaagcaaag caaatggagc
    31621 gaggcgtaaa atattttcca acagatcaat tgtacaaaga attgaaaaga cgttcagaaa
    31681 atcaagatcc attttctaac gaacgatatt atgaacggtt tttagaggaa atagctagtc
    31741 aggcaatcaa tgagttgcaa gaagaagtta tcgcgcttca gcatgattat gaatatttga
    31801 ggcaacaaca gcagatccgt cggttgccta cagagccatt ggagtgaaaa atatgtacta
    31861 tccttattta agaggaaaac aatttgacct attagcatta aaagaagcac tgtcgcgagg
    31921 attgctctcc aataaaatcc aaccagtgat cgaaccagta cgtgattccg ctacgcttaa
    31981 aaatgtgatc gaattatttc aaaaaaagca ccatccaatc gctgtgatcc aaaatcccca
    32041 agtaggacaa ttcaaattat tcgatcagca tgtgcacagt tggacagtca aagaaaatag
    32101 ctctgtcgtc cctgcacaga tcttaacgcc agaaaatctc agtgaagtac tggattcacc
    32161 tccagcgttt cttgttttcg atggacagca ctacccaaaa gatacagagg tgtggaaaca
    32221 gttggcagga gttgattcga aatttttgat accagatact agtcgctttc gcatctggct
    32281 gcccgaaaac aaaattgtga tccgtgactc ttttcaaacg agaaagcatg ttgaaagtta
    32341 tgcagataaa acagatgact tttttagtga tgactatctt tttttccacg cagacggata
    32401 tattggattt tctgatttca cgatcgaagg tagccgttat ttcgataaag ggttccctag
    32461 ccgagcgctt gctatccatc taacgtatat tgatgcttac ggcaatatac gcgtgaaaca
    32521 ttttgtctct gacagtaacg actctgctaa agaccaagca ttgaagtttc ttgaagcagg
    32581 agataaaatg aaaaaatgga tcatgcgtca ccatcatcaa ctcttgatta cttcaggaat
    32641 gattgaatta ttggctcttt accagcaaca aaaattccct ggactgggag tgttgaaaaa
    32701 gtggtcactt atgcatcatt tggaacttat tagtcacctg ctagatcatc caaaagattg
    32761 gcttaaatat tattcaaaag ttcaggaact atatgaagtg atccaatgat tagataatgt
    32821 aaatgaaaaa ggaatagaaa agaggaaact ggatgtatat tcggcaaaca aggaaagaag
    32881 aagtagaaga agtggtagaa atcattaatg ccgcagcgga tttcattgct tcgcaaggct
    32941 ctcctcaatg gcaaaacggg cagaaaccaa caaaagaaac gattttgaac gatattgaaa
    33001 gaaaagaaag ctatgtatta atagtggaag atcaaattgc tggaacagct gcattagtct
    33061 caagcgtgga tcccgtttac acaaaaatag atggtagttg gaaagaatca actggtcctt
    33121 atttatcgat tcatcgtgtc gctttagcag aaagtactcg aggaaaaaaa ctagcaaaac
    33181 cgttcttaga acatttgttg acgattgcag ccagcaaaaa tatccaagat atccgtatcg
    33241 atacttatcc agctaatgaa ccgatggaaa agaccatcta ttctgctgga ttcacttatc
    33301 aagggatgat tgaatttcct tttgaacacg gcgaaagaaa agcatatcaa ataatcatca
    33361 attgactata aaatcatctg agcaaagata aagacattaa cactactgaa aaaaacaatc
    33421 atatgatagc caagaggttg taacagaagt ggccagcttc aaaaaacaag aaagaatttc
    33481 cgaaaatgct tctcaaattt ttgtggaatt ttagcgtatt ttacgaaata gctgctttct
    33541 gaaactccct tcatcgagag gtcgtgacat agcttttgtc acgacctctc ggcttatcct
    33601 acgagtatta ttcgatacat ttatatcaat aatccttttt ttattttatc tgcagcttct
    33661 tggaacgctc catcgtttga cacgactaaa agtgagggga tagctttcaa acgtttcaac
    33721 tgtgctaaat cttctacata aaccgccagt ctggctgaaa tctgttcaga tgaatctcca
    33781 cgttttttca ggcgcgattg caatgtttct ttagagatcg taataaagat aggacggact
    33841 accgtcccga aatactcata taaatgccaa aaaccatttg ctgtcaaaac ggcgtaacaa
    33901 tttcctactg tcagtttctt tttaatttct gctttactgg aaccatattt atgttgagca
    33961 tattggtccc actctgcaaa ctcatttccc ttgatcatct gattgaatgt attgtcagaa
    34021 acaaaataat aatcctcttg atctttttcc ccaggtcttg gtgatcgatt cgtgtaagta
    34081 atgatctttt gttccggctg aaaaatagct tctgcgagtg aagtttttcc agaaccagaa
    34141 ggaccgataa atacatagca ataattcatt gtcatttctc ctttgatgat tgaacaagtg
    34201 aaaaagcttt ggaaaatatt tccaaagctt ttaaaagtaa ctttgctttt ttgtatatta
    34261 taccagcaag ccagtcacaa tagcacttaa aaagctgact aaagtcgctg ttagcaaaat
    34321 tttcaatccg tgttttgcga caagatttcc tttttcttca tttaatccct tcatcgcacc
    34381 agaaatgatc ccgatagaag agaagttggc aaatgaaacg aggaagacag atataattgc
    34441 tgttgtacgc tcagtaaaat ggaaattacc atttgctaat tctgtcatag caacaaattc
    34501 atttgtaacc agtttagtcg ccatgatact accggcatcc actgcttctt tccatgggat
    34561 tccgctgatg aaggcaagag gggcaaagat gtaacctagg atctcttgga aagtgatgcc
    34621 gaatatcccg tggaagatag cattgatcat ggcaatcaaa gcaacaaaac cgataagcat
    34681 agcagcaacc gtgatcgcca catggaaacc atcaagaatg tattctccca acatttggaa
    34741 aaatgtctgt tctttgtttt cttcaatgat cagctcatct tctttttctt ctaatacata
    34801 gggatttaca attgaagcca gaatataacc gccaaataag ttcaaaacaa gggcagtgat
    34861 cacatattta gaatcgatca gtgccatata agatccgacg atcgacatag aaactgtaga
    34921 catagccgat acactcatgg tgaacaaacg tttctcagat aggaagggta attccttttt
    34981 tatggaaata aaaacttccg attgtcctaa aatagcagaa gctactccat tataagattc
    35041 caatttgccc atcccgttga ttttacttaa tcctagacca acagctttca taaataatgg
    35101 aagaatcttg atatagcgta gaataccgat aatagcagaa ataaatacaa ttggcatcaa
    35161 tacggaaaga aagaaaggtg cacttccttt atttgctaca ccggcaaaaa caaagtcaac
    35221 accttgccca gcaaaagcaa gaagatgatc aaacattttt gccaatacag aaacaaccgc
    35281 tgtaccaaaa gttgtacgta ataatacgaa accaagaaca agctgtaaaa cgaacatcac
    35341 aattagtggt ttcaatttga ttgctttttt gttactgctg atcaaaaaag caagccctaa
    35401 tgtgacaaat aaaccaataa tacctaataa ataactcaaa taatcactcc tattccaaat
    35461 tccgttttat tttacctgtt ttgcaaataa aatactaatc tttagtgaaa ttttcattac
    35521 ttttttagag tgaaacgcat tcattttcca ttagaaaata aagcgaacaa aataaaaggt
    35581 ttaaaaagtg taacgtctta gtaaagtcta taaaaaaacg aaactatcga ttaagctaat
    35641 agtttcgttt tcaacattta taagtaaact ttttctattg gtaatataga ttttcggaag
    35701 ggtagacagg tgcgcaggta atatcgattc ctagtttttt caatgtttgt tcatcatttt
    35761 tattgaccat cacagtagag tgggcttgtg ctccctcaag ttcagctagt ttatcataag
    35821 caacttgagc gattggattc gttactgcac tgattgataa agcgatcaag acttcattga
    35881 tgcttagtgc atccaaagag gaatgaagtg tctttgattt caatccttgt attgtttcaa
    35941 gaactaatgg tgacaataaa ggaatgtcat ctgcgatatg ggctaaatgt tttactgcat
    36001 ttaagataac tgccgctgac gcatccatca atgaacttgt acgcccggta atgatgactt
    36061 catctggtaa ttcgattgca atcacagcag aaggatcttt tgtaggactt tgcgcttgaa
    36121 tcttatctgc atagtcatgg gcagggacta ccacgtttcg gtcttcagga cgtaaaccga
    36181 cttcttccat aatcagtttg attcgattaa ctgcttcttc gtcgatcaat ccttttttga
    36241 agtcgcattc tgtttggaaa taacgacgga tgatttcttg tttagaggct tcttggacaa
    36301 cggtatcatc cacgatccca aatcctacac gattcacacc catgtcagta ggagattgat
    36361 aaacagattc tttaccagta attttttcaa taatacgttt gattacagga aatgtctcaa
    36421 catcccgatt atagttcaca gctactttat tgtaggcatc aaagtggaag gaatcgatca
    36481 tattgacatc ttttagatcg actgtggctg cttcataagc aatgttcagc ggatgtttca
    36541 acggaacatt ccatactggg aaagtttcga acttggagta accagctgca tgtccttttt
    36601 ggctttcatg atacagttgg ttcaaggaag tagcgagctt tccacttcct gggccaggag
    36661 ctgtcactac aacgattggt ttagttgttt caatatactc attttttcca aaaccatctt
    36721 ctgagacgat tttttctacg ttgactggat agtcttcgat ttctgagtgt ttatagacct
    36781 tgatgtttcg tctttctagt ttattgataa agactttcgt ggcaggctgt ccactatagc
    36841 gagtgataac gacactgttg attaataagc cataaccacg taattcatcg attaaccgca
    36901 agatatccat atcataagta atcccataat caccacggat cttattacgt tcaatatctc
    36961 cagcatagac gcaaatcagt atttcagctt gatctttcaa tttttgcagc aatttgattt
    37021 tcgcatcttc ttcaaatcct ggaagtacac gtttcgcatg cttgtcgtca actagcttcc
    37081 caccaaattc taaatataat ttgtcatagt ggttcactcg ttccaaaata tacgctgact
    37141 gctcttcaat atatttttct gaatcaaaac caattttttt cactaggttc cctccttaag
    37201 tactaaagca ttttttcatt tttttgagga ttcgtcaact ggaagagcga aattcctcaa
    37261 aaaaacaaag aaaatggttt aaaccaactt gtctagatag ttattcattg gtatgatagt
    37321 ttttgataag gaaggtgcta catgaaaaaa agacagaaaa agaaaaacgc atataaacac
    37381 tatatccgtt cgatttttac cggttatgaa agaatgcttg aagatcccga attggaacaa
    37441 ttgacgttta cttatctaaa cgaagaaacg cagctaactc gtgatgagca tcagcgcatt
    37501 catttcacaa caagagatct gcctagtaaa taaaaaataa gataaaggtc atcggattcg
    37561 attctgtcct atcagatgga aagactagtt gatcgaaaaa aaggctgtac caaattttgg
    37621 tgcagcctgt tttatggagc attgcccgct cttttttata acaatttttt acagaaaatg
    37681 gtactattga agtattaata agtgaaaaac agatgagtaa gaggaggcaa gtagtatgaa
    37741 agagccagat acaaatgcaa attatgagcc agacgaacta gaggagacga atgcggatca
    37801 ggatacagga caagaatcgt cttcttttta cgatatcaaa gaaaaagcaa gcatgaatct
    37861 ttcccaatta ttagaagact tacaggcttt tgaaaaagct gtccgcgaag aagatatccc
    37921 tgagatctac cgtttataca atggccgatt gaatgaagaa ttaaaaagaa gctcaaatga
    37981 aaatcacgag atcgatcaat tgctgatgcg taagatccat gatcgttttt tacaggaatt
    38041 tccttttatg gaacaagtag aaaacatctc gccaacgatg agctattaca aaatcggcac
    38101 ttattaccat gatcggccca cggtcggtat tgatgcaagt ctgccagaaa tttttgtttt
    38161 gcctaagatc gatgaagagt gggagaaata ttcccaaccg gagtcaacgg actatgacaa
    38221 aaaaatcaat gagctagacg caaaggtgat cactgctcag acagaaattg aacgtttaga
    38281 tgaacaaata aaagaaatca atcgacaaat gactgatttg aacgacaata aaggattctt
    38341 gaatcgtaaa aaaatcgagg aagaaattca agaattagag aagaagaagc aagtactatc
    38401 taatgaaaaa ttaggctggc ttccttatat tgagacgcct gaaacaatcc agcagcaaaa
    38461 agaagcattg aagcaagaag caagagcaga ccagcttcgt gcagccatcg tagaaaaaga
    38521 acaaaggcaa atcagacgct atttcggtag caaagaggga ttcggtcaag caatccatga
    38581 attcttgatg aattatcttg gcaatgagaa tcctaataat caatctaatg aaggaggttc
    38641 cgagtatgaa tgaacacgat gaagcagaat ggacaaaaaa tgaagaacaa acagaagatc
    38701 atgaagaaac gaacacttca ttggaagaac aaacaagtaa tcctctggat gacacagaag
    38761 aaatccctat gctagaggaa acaaaaatac acgaaaaaga aaaagtaact ccgacgaata
    38821 cgaatactgc agaaaacacc catgcttacg aaataactga aaatcccggg tatttagaag
    38881 gaaaagagca agatcatcaa gatatcgtca gtttgattga aacagcggaa gcagaattag
    38941 cggatttacg tatgagaaaa gatttgatcg aatcagattt tccacaatta ttagaatcat
    39001 atgatcgttt tatattgaat gaagaaccaa tctccacgat cggaaagaca agcctgatcg
    39061 aatacttcac gtatgaatta ctgaaaccgt tgaatgatat tggtctggaa cggtcaaatt
    39121 cagcttatga cacatggaaa acaagacatt tttcgatcaa ttatacgttg aatggacaaa
    39181 acgaattagt cttttccttt gtattaccga ccatcgatca acgataccaa gtggaatcga
    39241 tgagtctcct aaacgtatca ccagagacaa tggaaatcaa tgtccaagat gaccgggtgc
    39301 tttctttgat ccgacactgg tcagtggatc gagtattctc tgcagggcag atcacgatat
    39361 tcaatcacaa attgaatcaa ttattagccc atgcaagaac acttggtttt accgttaatc
    39421 agacattact agacaatacg aagccacttc gtttgaatct tcagagcgaa tttgaattga
    39481 cgaatgaagt attggatgat atttttatcg tggcaatgaa aaatccatcc tacgatatgg
    39541 aaaagatcaa cgaaactact tatcaagtac gattagataa aggacagtct ttaacgataa
    39601 caacaaacga aaaagatcaa acagtcttgt cgatctcatc tggagactac ccacgatctg
    39661 tcattgattt ctttatcaat tacgaattct tggtcccatt aatggtgaga aagtcctgat
    39721 tggataagtt ggattaccag atgtcattca gataattctg ttccaaagga cttgcggcag
    39781 ttatccagaa taattgtcac aagtcttttc tgtatgtact aaatggacgt gaataacttg
    39841 ctgatgaagg agggatttca ttaggaatgt ccacaaataa aatagcacag cttagcttat
    39901 tgtctgctgc ttgtgtagca ggtaggatcg cttttcaatt tattccgaat ttccagccaa
    39961 tgactgctat ttttttattt atagtacttt acttaagctt aaaagatgcg ttaattgtca
    40021 tgagtctttc tattgcgatc agcagttttt attttggtgt tggaccatgg gtcatcggcc
    40081 aatggatcag ctatactgtt gttctgagtt tgttttcaat cgtctgcttg agaaaaacag
    40141 tgcaaaggaa tctatggtta aagggagtct gtttttttct ggcgggcatg gtttacggaa
    40201 taggtatgac cgtttttgat acgctgttat atcgtttgcc tcaaccttgg atctattacc
    40261 ttcaaggggt gtcgtttgat ttgatgcaca gtataggcaa tgttgtgttc tttcttgttt
    40321 ttctgccagt cgtcaaacgt ttttacaatc attcaggagg aaaaaatgaa aaaaataatt
    40381 tatagtttgc ttgcaatgag tgtcagtata ctcattttga caagttgcag tacaacctat
    40441 acacaatcgg cttccaacca aaaggaggaa gtaaaaacat cgcaaaaagc aacaatcacg
    40501 ttacaagaaa atggtcaaaa cttttcggaa aaagaagtgt tcttcaaaaa aggcgatgat
    40561 ttactgagta ttttgaaacg aaattttgaa gtcaaagaag ataacggctt catcacttcg
    40621 cttgaagggc actcacagga tgagaaaaat caactctatt ggatgtttac tgtggacggg
    40681 aaaagtgcta ctactggtgc caaagagatc aaattgaatg atggacaatc ggtcattttc
    40741 aatttgtcta aactataagt ttcataaaaa actgttaaag ggcgaatatt gccataattc
    40801 agtgagttca gttgatgaaa gaaggaaaat aacatggaaa attttaaaaa aagcggaatg
    40861 ttttccgtaa ttgctgctct aggggctaat attctagttg ctatcagcaa atttgtcggc
    40921 tatgcaatca gtggcagtgc cgccatgcta aacgaaagca tccacagtat cgttgattgc
    40981 agcaatcaga ttttgctgtt attcggtgat aaacaagcca ataaaggaca gagcgagttg
    41041 catcaatttg gtgaaggacg agcaaaatac tttttcagta caatcgtcgc aatgatgctt
    41101 ttcttcggcg gtggggcact tggggtgatg gaagcttttg aaaagttgac gcatccatcc
    41161 catgaagtgg gtaatccgat cattgttata gcaatactgt tatttggttt ggtaatcgaa
    41221 ggaagttcgt tacgcgtggc aatgaaagaa atcaaagaat tgaatactga aaaactttcc
    41281 acgatgcgct ttttgcgcga aagtcgacac agtgaaatac tgattatttt tacggaagac
    41341 ttgtgtgctg taatcggttt ggtcttggct ttagcaggaa caatattgac ttatgcaacc
    41401 gggattgctg catttgatgc gatcagcgga ttgctgatcg gtttcttgct gatgggagcc
    41461 gccattttcc tagcaaaaga attttatagt ctgatcatcg gtgaaagcgt gacagcaagc
    41521 gatttatcaa aaatcaagtt agcttttgaa cgtcctgaga tcagtcggct gatcgatata
    41581 aaaacagttc atttaagtcc aacggagatt ttagttgcag caaaagcaga tatcgttggt
    41641 aacaaagaga atgaagctta ctcggtcatc aatgatattg aaaaaaatat gcgtcagtct
    41701 cttcctgaca agaagatgta tatatacatt gaaacagata aatatgaccc aaattattct
    41761 cgtaaataaa acgtttattc atgaattgaa gcaaaaatga gaaaaatata atcttgtttt
    41821 taaataaaac ggctacaatt tagcgaattt atgttacaat gagtccgctg tttatttgtt
    41881 gtaattaaag gagatatttt aatgaacaaa aagaaaagtg cgccatggct tcaagctttg
    41941 catgcagtga tgccactttg tataagctat ataccagtag gattagcttg cggagtattg
    42001 ttgcaaaaag ttggatttga tcctctctta tcaggactgt tatcgattct ggtcttttcc
    42061 ggtggcgcgc agtttttagt tgcatctatg cttacgacgc aagcatcatt tgcaacgaca
    42121 ttattgatgg ttttcttttt agaattacgc tatacattat taggagcaag tcttgccggt
    42181 tttatgaaaa aagaaaagcg gacgtttttg gctgtctttt ctcaatcact gaatgatgaa
    42241 aactatgcgg tgaactactt aaagttttca actgaccctt cctggaataa aagaaaggct
    42301 ttatatgtca attggttctc aatgactgca tgggccggta gcaatatgat cggtaatttt
    42361 ttcggctcgg tgattcgagt agatgctgac cttgtccatt ttgctttgac ggccatgttc
    42421 attttcatgt ttatcatgca gatgaaaagc gcactactga tctttacagg tttattctca
    42481 ggcgtattag gggtcgtatt catggtatta ttccaaaata ctttcggatt gattgcagca
    42541 acagtcattg cttcatttgc cggatttatg ctggagaagg cattcaaaaa agaaaaagct
    42601 aagaaactaa gagaccgcgg taaagatgtc aatgagccgc tcttcaactt cccggaacag
    42661 gaggccaatc accatgacta aactatattt gctactttta gttgcttgtc tgttcatcgt
    42721 ggcatatatc ccgcgcttgt tcccgatgct ttattttact catcgaaaag tgccatcctg
    42781 gttcagtgac tggatgaaat atgtaccggt ggcattattt gcggcgttag cttttaaaga
    42841 tgttttcatt actcatgaac atctggatat tgcttggaat atcaaaatag ctgcaatgat
    42901 tctagtagca ggcgtggctt acaaaacacg ttcgatggcg ttgtcagttt tgacaggact
    42961 tgcgtctgtc tttttgctgt ctatgcttta aactgtttta ttttattaaa aacagagatt
    43021 gcgacataag ttgttcaaac tgatctatcc gaactgcatt tgcaacagtc aattctggat
    43081 gcaagtttat tcgtagggat aaaaaagatt agtgttggct tatgatgcaa gctctttttt
    43141 attttaggaa ttagagaggg atttgtcctt ctatgatttt ttgctataat agggaagatg
    43201 tttattttat gaaagaatag ttgagggaat ttaaatgaat aaaaaattga tgcaaggaac
    43261 attttggcta acctttgcta atcttctatg taaagtttta ggggtagtct atttgattcc
    43321 ctggctaagt atgatgggga acaatcaaga tggtatgctt gctacgacat tgtataatgt
    43381 tggctatctt ccttatggat tgttcctgat gcttggtaca gtcggatttc caaatgcgat
    43441 tgcaaaaaaa gtagctgtag ccacaaaaga aggagataac gctgcttgtc ggctgatttt
    43501 tagaagtacg atcaatatca tgtttgtcat cggtatcgcc tctgctgcac tgatgtattt
    43561 atttgctcct ttactagccg gaatcagtcc gattgctaac gttaataatg ggatactagc
    43621 tatcagaagt ttatgcccat ctttgattgc tatcccaatt cttagtgcaa tgagaggata
    43681 tttccaagga aaaaatgatc tgcggcctta tggaacttct ttgattatcg aacaggctgt
    43741 gcgagtgatc gttatcttgg ctggtactta ttacttgcgc gtattgactg acggaaccat
    43801 attagaagct gtattgatca gtacagtcgc ttctttcttc ggtgggttag ctgcgattat
    43861 ccatatgtat atcgtcggac gcagaaaaga cttcttcgaa cttaaagatt ttttgatttc
    43921 cagccgttat ttcgaaagag aaaaccgatc tgcctctgtc tctatcattc gggaaacact
    43981 accgtttatt tttgtcggct cagtcattac tatcgttcaa atgatcgacc aagtaacgat
    44041 gaaaccactg cttcactttt tccgtccaga gatcgcagat cagcaactgg aattcttatt
    44101 cagccgtgct tctgtcaatc caaataaatt gacattgatc ctgatctcca tggtaggaac
    44161 agtagctata agcagcttgc caatattaag cacgatgaag aaaaaagatc ggatacaaat
    44221 cgaaaaaaca gtaggagata gtttttcgat tgcgttattg attcttcttc cttcattgac
    44281 tggaatgtct ttattagctg gaccactata cacattgttt tttggctatg atcctgaaag
    44341 tgtcggatat ttccaaatgg ctttgctagc ttctttgttt ttctctttat tcacgatcct
    44401 atctacgatg cttcaatctt tgaatcacca tcgttttgcg atccgattaa ctgtagaagc
    44461 aattatcctg aaagtcatct tccaagtaat cggattagga ttagttggcg gatacgggat
    44521 gagcttatca aatggcttag cattcggtat tgtctttgtt cgcggctatc actttatgtg
    44581 caaagaatac cgaatagcac ctttagcaaa gatcagcaat tttttcttaa aaactttccg
    44641 ctccaccctg attatgcttg cagtatgttt tgccgttttc tttttgctga atcagcgatt
    44701 gtcaatggaa tcaaaaagct atgcaatgat ttattgtgtg gctgtaggaa gtattggcgg
    44761 cttagtattt ttattttctc aattcggaaa aaatgggttt cgaatgttga aaaatgttaa
    44821 acatcatagc agaagcaaag aataggttaa ctaatcgttt tttgctgtca cttgaaaaac
    44881 ggcaactcgt atgattacgc ttgtcgtttt tctattttcc aattttagtt tcatatctaa
    44941 tcttacttat gatatagtta taaggtctgg aaaaattctt tgaaacgagg aaattaaaca
    45001 catgaaactt gcagtagtta cagatagttc ggcatatctg ccggaacgta tcaagaacca
    45061 tcccgactta ttcgttattc caatcccttt gattatggat gggaaaattt ataacgaagg
    45121 aatcgatatc gaagcggatg aatattatgg acttttaaaa aacagtaaag attttcctac
    45181 tacttcacaa cctgtcgtag gggaagtttt gggtttatat gaggaattga aagagaaagg
    45241 gtatgacacg attttaagta tccatctttc ttcagggatt tctggctttg tcaacacatt
    45301 atttgcgatg aaagatgaca tcagcggagt aacggtcatt ccttatgact ctaagatcac
    45361 tagtatgccg atgggacaca tggtagaagc tgccttagac ttaaatgatg aaggtcagtc
    45421 ccttgatgaa atacttgcac atatcgatcg aatccgagat aatacctacg cttatctgat
    45481 tgtagatgat ttgaacaatt tagtacgagg aggacgtttg acaaatggtg cagcgctgat
    45541 tggcagctta ttaaaaatca aaccgatcct gacattttct gaagggaaaa ttgttttatt
    45601 cgaaaaaatc cgttcaagta aaaaagcatt tgctcgagct gaagaaatta ttggtagaag
    45661 aaataacgaa atacaaccgc cagtcaaact ttacgtgata catgcaaata atttagaagt
    45721 agcgattgcg gagaaagaga aattgcagaa aaaatatcca aatgcagaaa tcgaaattgg
    45781 tcatttcgga ccagtcattg gtacacattt aggtgaaaaa gccatcggca ttgccatttc
    45841 agcgcaataa ttccttttat tcaaaaaaca taccaaaaat attccaacat catcttggac
    45901 tttctacatg ttactaatga gcgtgtaaaa gaacgagatg gtgtttttat ttctatcaat
    45961 gaagctcttt ttctttgcaa tcctcacttc aatcctctat aatgtcgcat atagtttagt
    46021 aaatgagtaa actactaatt tacttattta ctaaaaataa aaggatgagg agttaaaata
    46081 atggaaatac caacaaattt aaaacataaa ccagtaatcg ttgtagaaga ctatgataaa
    46141 gttgatggaa gaaatgcatt aaatacggat gcaaaagggc tttcactggg ccttgctcag
    46201 tggaatgatc gtggaaaagt cgatatctca ggcaaggtat ggcgacatac tggagaaaaa
    46261 tggtcacgac agtcggaaga attaccaatc acacgtattt tagatttagc gatcttagct
    46321 gcacaaggaa gtttatactt ccaagatgct tatcgttatg aaaaatttta tgatccagaa
    46381 aatccagtcg tagacatcat tggattacaa gggaaccgaa tgacggtaga agtagatacc
    46441 aaaaatccaa agatcgatga agacatcatt ttgtattacg atacactaca aaaagacgga
    46501 gaactgattg gggaacgatt ccgtatttta aaacgtcttt tagatgagct aggatattaa
    46561 tagacagaga tgatcaaaag gggaagagaa gcatggagaa tttctcacta acagaaaacc
    46621 gaaccttttc acggctagaa aagcaaatga tctggaagaa accgaaaagc catcggacaa
    46681 gtgcagaaga atggcggatc gcctccgaaa tcaaagggaa ttggcaagat cctgaaatga
    46741 aaatcagtaa cgtacttttg gaaggagatg ccgggtcggg gaagacacaa ttagccaaag
    46801 cactatctgc tgatctgcaa cttccctata caaaagtgac ctgttttgca gatatggaca
    46861 aatcagatgt tttcggagcc ttattacctg tagtagattc taataaggaa caagaccaag
    46921 aattactaga agctatttac caaacagata cacttgaagc tgtactgaca ctgatccaga
    46981 accattacgg cattgatcct ttttctgcta aagaaaaatt agcagagcta gttcaaagga
    47041 tcgaagctga ttatccagat gcaatccatt acagctatta cccttctgaa atcgttcgag
    47101 cgattgaaaa aggatattta ttagagattc aagagccgac tgtcatccga gatgcctctg
    47161 ttcttgtggc acttaactca gcgctcgaac caaatgggat gttgaattta ccgacaggaa
    47221 tcattcgtcg ccatccagat tgcgtcattg tgatcactac aaatcgaaat taccaaggaa
    47281 atcgcccatt aaatgaatct ttaagagatc ggatgcaaca tgcggaaaaa atggatcttc
    47341 ctgccttaga agtcatggca gaacgtgcga tggcgaaaac tatgatccaa aaacaagaac
    47401 ttctcttaaa gatggcagag attatccgtt tgttggatga aactgcaaaa gctaacgcca
    47461 tcaaaggtgt agcaggaatg cgttcttatt tttattgggc aaatacctat aagcaaggac
    47521 aagatttact ccagtcgatt tatccaaaag ttttgtataa attatccact gattcagatg
    47581 aattggcaat tcttgaacaa gctttgtctg acagtggatt gctactggaa cttcaggaca
    47641 tattgagaaa agaaaaatgg ggtcatcctc aatctgagca tacaaaagga agaaccatta
    47701 gcgccgaaga agcaaatgaa cgaaacatca ctgaaaatgc tgaagaaatt ctgcgcttag
    47761 atgaaaagaa atcgttgtct gctgaagaac caaatgaggg agaagagaaa caagaagaaa
    47821 caatagaaaa aaacgagatt cctcctcaaa tgcaaaaaga acgcagtagc gatatcgaag
    47881 gaaaaaatca aaaagaacaa caaacaggag atgattctca agaaatgtct gcttctgaca
    47941 tggaagcaca tgataaggaa atcaagaagc aactgaataa agaagcgcga caaatcatga
    48001 aggaaacgat tcatgaaaaa gaaggtctca tcgttcaccg accagctttc caaggaaaaa
    48061 cgccggaagc acgtgagcta cacatgcaag taatgccaat agtagaatca cttagccggc
    48121 aaattttaga gttactggat aatgaacaaa cagagacgta ccaaaaagga aaatacgaag
    48181 gtcagcggtt caatgcaagc aaagtagcat atggtgattt gagaaatttt gacaaaaaga
    48241 acccgccaca tgagcaacct tctttagcgg tagctgtacg gatcgatgaa tctgggtcaa
    48301 tgatacggga tgatcggatc gaagcggcaa aaaaagcagc gattgccatt gccgaatttt
    48361 ctaagaaagt cgatatccca ttgttgattt acggagatac agcagaccgt tcgtcaagag
    48421 aaaagacttc tttgttttct tataaagaat ttgaagacgg atttcaatgg ttaaatgaaa
    48481 aattagtcac tatgaaacca aggcaaaaca atcgtgatgg tgctgcactt cgtttagcag
    48541 cagaaaaatt aaacagacaa tccgcaacaa ccaaactgtt gcttaatatt agtgacggtc
    48601 agccaaaagc gttgccggat tatacaggca aaaaagcaaa agaagatatc cagtctgttt
    48661 tgaaagaata tgaacgtcaa ggaatcctat ttgtctccgc agctattggc caagacaaag
    48721 aagaaatcaa gtcgatctac ggtgaaagtc gttttgtaga tattactgac ttgaatgaat
    48781 ttcccaagca aatgattcaa ctgattgccc gctatctatg ataaaataaa gctcaacgaa
    48841 cgcacttagc agattcgttg agcttttcgt ttctagttac cggtaacaag gatacttcta
    48901 aaatagaatc ggttataatc aagagggacc gttaaaggag tgaaacgata tgatacaaga
    48961 acaaaaatgg tggaaagaag cagtaggcta tcaaatctat cctgctagtt tcaaagatag
    49021 caatggcgat ggtcaaggag acatcaatgg gatccgtgaa aaacttaatt atttgaaaga
    49081 tcttggtatc ggctttatct ggatcaatcc gatctatcaa tctccatttg tcgataatgg
    49141 ttatgacgta agtgactatc aagatataga agagagattc ggaacgatgg aagattttga
    49201 tttattgcta aaagaagcac atgaattagg gatcaaagtg attatggatc ttgtcatcaa
    49261 ccatagttcg gatcagcatc aatggtttga agaatcgaaa aaatctaaag aaagcccgta
    49321 tcgcgattat tacatttggg tcgacggggt agatggcaaa gaaccgaata attggacatc
    49381 gattttcggc ggttctgctt gggagtattc tcatgaaact ggacagtatt atctgcatgt
    49441 ttttgcaaaa gaacaaccag acttaaattg ggaaagcgag aaattgaaag aagaactatt
    49501 caatatgatc cgatggtggt tggataaagg gatagatgga tttcgtttag atgcgatttc
    49561 tcatgtaaaa aaagatgaat attcagtaaa agccacagag aaccctttct caccctttca
    49621 aaatgtctca ggaatcgaag aacatttgac cgatttgaaa catgtttttg aagagtacga
    49681 tatcatgact gtcggagaag caagtggtgt cactgcagaa gaaggaccac aatgggtagg
    49741 gaaagacggt tatttcgata tgatattcga atttgatcat atccatatct ggcagcaaga
    49801 aaaagaaggg caattagatg tccttaaatt gaaacttgca ttaagcgctt ggcaaacttc
    49861 attggatggt atcggatgga acgctttata tatggaaaac catgatgttc ctcgtgccgt
    49921 ttcagtgttt ggagatactc gtcccgattt ttgggctatg tctgcaaaag caatcgctat
    49981 gatgtatttc tttttgcaag gtacgccatt tatttatcaa ggacaggaaa tcggaatgac
    50041 gaatatgccc tttgaatcga tcgatcaagt tgatgcagta gattcgaaaa gactttataa
    50101 aaggctgctt gcggaaggta agacaagaga agaagcattg gatatcattc gtgaaacaac
    50161 gagagacaac agcaggacac ccatgcaatg gacttcggaa caatatgctg gtttttctac
    50221 acatgaacct tggctgatca ctaatccaaa taccaaaacg atcaatgtag aacaacaaga
    50281 atatgaacca gagtcggtcc tgcaattcta taagaacatg atccgcatta gacaaaccaa
    50341 taaaggtctg atatacggtt cttataaaga atatcttcat gaacatccac aactttatgt
    50401 ctatgaaaga tatttagaag atgaacaata tcttattatg gtaaacttaa cagaatcact
    50461 tgctgactat gaacttccaa aagaagcaga tcagtcctgg acattactct tgtcaaattc
    50521 ctctagcgga gaatttgaag ctaaaggcat actagcacct tatgaagctc ggttatataa
    50581 aacaaacaag taaaacttcc tcgttaaa
//
LOCUS       DAZUKC010000009        50061 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000009 DAZUKC010000000
VERSION     DAZUKC010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.009"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..831
                     /gene="pnuC"
                     /locus_tag="ACG80T_000494"
     CDS             82..831
                     /gene="pnuC"
                     /locus_tag="ACG80T_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFS4840176.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(1015..2292)
                     /locus_tag="ACG80T_000495"
     CDS             complement(1015..2292)
                     /locus_tag="ACG80T_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840177.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(2371..3015)
                     /gene="pcp"
                     /locus_tag="ACG80T_000496"
     CDS             complement(2371..3015)
                     /gene="pcp"
                     /locus_tag="ACG80T_000496"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFS4840178.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(3214..4158)
                     /locus_tag="ACG80T_000497"
     CDS             complement(3214..4158)
                     /locus_tag="ACG80T_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFS4840179.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(4160..4846)
                     /locus_tag="ACG80T_000498"
     CDS             complement(4160..4846)
                     /locus_tag="ACG80T_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFS4840180.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            5320..5640
                     /locus_tag="ACG80T_000499"
     CDS             5320..5640
                     /locus_tag="ACG80T_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFS4840181.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            5794..6699
                     /locus_tag="ACG80T_000500"
     CDS             5794..6699
                     /locus_tag="ACG80T_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4840182.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            6696..7469
                     /locus_tag="ACG80T_000501"
     CDS             6696..7469
                     /locus_tag="ACG80T_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS4840183.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(7650..9074)
                     /locus_tag="ACG80T_000502"
     CDS             complement(7650..9074)
                     /locus_tag="ACG80T_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFS4840184.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            9285..9731
                     /gene="nrdI"
                     /locus_tag="ACG80T_000503"
     CDS             9285..9731
                     /gene="nrdI"
                     /locus_tag="ACG80T_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFS4840185.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(9796..11778)
                     /locus_tag="ACG80T_000504"
     CDS             complement(9796..11778)
                     /locus_tag="ACG80T_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFS4840186.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(11792..12688)
                     /gene="murQ"
                     /locus_tag="ACG80T_000505"
     CDS             complement(11792..12688)
                     /gene="murQ"
                     /locus_tag="ACG80T_000505"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFS4840187.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(12691..13752)
                     /locus_tag="ACG80T_000506"
     CDS             complement(12691..13752)
                     /locus_tag="ACG80T_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFS4840188.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(13963..14490)
                     /locus_tag="ACG80T_000507"
     CDS             complement(13963..14490)
                     /locus_tag="ACG80T_000507"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS4840189.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(14557..16059)
                     /gene="eat(A)"
                     /locus_tag="ACG80T_000508"
     CDS             complement(14557..16059)
                     /gene="eat(A)"
                     /locus_tag="ACG80T_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFS4840190.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(16317..17437)
                     /locus_tag="ACG80T_000509"
                     /pseudo
     CDS             complement(16317..17437)
                     /locus_tag="ACG80T_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            17563..18504
                     /locus_tag="ACG80T_000510"
     CDS             17563..18504
                     /locus_tag="ACG80T_000510"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFS4840191.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(18654..19550)
                     /locus_tag="ACG80T_000511"
     CDS             complement(18654..19550)
                     /locus_tag="ACG80T_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFS4840192.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            19819..21159
                     /locus_tag="ACG80T_000512"
     CDS             19819..21159
                     /locus_tag="ACG80T_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4840193.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            21377..21820
                     /locus_tag="ACG80T_000513"
     CDS             21377..21820
                     /locus_tag="ACG80T_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFS4840194.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(22324..23742)
                     /gene="nhaC"
                     /locus_tag="ACG80T_000514"
     CDS             complement(22324..23742)
                     /gene="nhaC"
                     /locus_tag="ACG80T_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFS4840195.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(24145..25368)
                     /locus_tag="ACG80T_000515"
     CDS             complement(24145..25368)
                     /locus_tag="ACG80T_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFS4840196.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(25399..26238)
                     /locus_tag="ACG80T_000516"
     CDS             complement(25399..26238)
                     /locus_tag="ACG80T_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS4840197.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(26255..26935)
                     /locus_tag="ACG80T_000517"
     CDS             complement(26255..26935)
                     /locus_tag="ACG80T_000517"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFS4840198.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(26937..28010)
                     /locus_tag="ACG80T_000518"
     CDS             complement(26937..28010)
                     /locus_tag="ACG80T_000518"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFS4840199.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(28066..28304)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(28347..28580)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(28657..29328)
                     /locus_tag="ACG80T_000519"
     CDS             complement(28657..29328)
                     /locus_tag="ACG80T_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4840200.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(29415..32063)
                     /gene="mgtA"
                     /locus_tag="ACG80T_000520"
     CDS             complement(29415..32063)
                     /gene="mgtA"
                     /locus_tag="ACG80T_000520"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFS4840201.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(32405..32579)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            32752..33189
                     /gene="ndk"
                     /locus_tag="ACG80T_000521"
     CDS             32752..33189
                     /gene="ndk"
                     /locus_tag="ACG80T_000521"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFS4840202.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(33211..34077)
                     /locus_tag="ACG80T_000522"
     CDS             complement(33211..34077)
                     /locus_tag="ACG80T_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS4840203.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLSHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(34237..36330)
                     /locus_tag="ACG80T_000523"
     CDS             complement(34237..36330)
                     /locus_tag="ACG80T_000523"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS4840204.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(37079..37702)
                     /locus_tag="ACG80T_000524"
     CDS             complement(37079..37702)
                     /locus_tag="ACG80T_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840205.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHVGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(37861..39258)
                     /locus_tag="ACG80T_000525"
     CDS             complement(37861..39258)
                     /locus_tag="ACG80T_000525"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4840206.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(39290..40261)
                     /locus_tag="ACG80T_000526"
     CDS             complement(39290..40261)
                     /locus_tag="ACG80T_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4840207.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            40635..41060
                     /locus_tag="ACG80T_000527"
     CDS             40635..41060
                     /locus_tag="ACG80T_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840208.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(41548..42249)
                     /locus_tag="ACG80T_000528"
     CDS             complement(41548..42249)
                     /locus_tag="ACG80T_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFS4840209.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(42265..42705)
                     /locus_tag="ACG80T_000529"
     CDS             complement(42265..42705)
                     /locus_tag="ACG80T_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFS4840210.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(42705..43280)
                     /locus_tag="ACG80T_000530"
     CDS             complement(42705..43280)
                     /locus_tag="ACG80T_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFS4840211.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            43521..43730
                     /locus_tag="ACG80T_000531"
     CDS             43521..43730
                     /locus_tag="ACG80T_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFS4840212.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
     gene            complement(44128..44640)
                     /locus_tag="ACG80T_000532"
                     /pseudo
     CDS             complement(44128..44640)
                     /locus_tag="ACG80T_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(44766..45467)
                     /locus_tag="ACG80T_000533"
     CDS             complement(44766..45467)
                     /locus_tag="ACG80T_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4840213.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(45575..47018)
                     /locus_tag="ACG80T_000534"
                     /pseudo
     CDS             complement(45575..47018)
                     /locus_tag="ACG80T_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            complement(47032..48897)
                     /locus_tag="ACG80T_000535"
     CDS             complement(47032..48897)
                     /locus_tag="ACG80T_000535"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS4840214.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(49018..49869)
                     /gene="licT"
                     /locus_tag="ACG80T_000536"
     CDS             complement(49018..49869)
                     /gene="licT"
                     /locus_tag="ACG80T_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFS4840215.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
BASE COUNT        14456 a        10561 c         8672 g        16372 t
ORIGIN      
        1 ttttttcaag aaaagcatac aatagattgt gttagtaaat acagaacaca ctacatttag
       61 tgtcttaagg agtcctcata catgaagtca tattttcagt ttttagctca tcagctaaaa
      121 ggctggccgc aacaaaatta ttatcttttc tttttcagtc ttggctgcca ggtcatgact
      181 cttgtcaata gtcccatcac ttggctgtcc gttattacat ttatcggtac cacgcttggt
      241 gtattgtgta ttttatccat caatgcggca aaatcagtca acggtatttt aggtattttg
      301 tctgcgattt gttttattat cgttgggttt tcagcaaaga actatttaag tatcggtgaa
      361 cagattgctt atatgatcac attggatatc cccgtattgt taagtaaaga atggaatcat
      421 aatatggctt caaaaatccg aaaattcact ggaaaaagct ggtttatcgc aatcatcagt
      481 actttccttg tctatcttgt ttcaggattc ctgattggtc gttatacaga tgatcctcgg
      541 ccgtgggtag atgccattgc tttttctatt agcttgactg ctggtatcat cagtttcttg
      601 cgttacaaca accaatatta ctggtggtta gcttcagggc ttgctcaaat ggtcctttgg
      661 tatatttcat ttacgcatgg atctgcaagt ttggcgatgt tcgtgaacag ctctgtttac
      721 ttgctgaatg atgtccttgc atttaccgtt tcaccttggt ataaccaaaa agaacgtgaa
      781 cgtatgcaaa aaatggaaga agaatactac aacgcacaac aacacgcata aaaaaactga
      841 gcgtgggaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat aaacggcgga
      901 atcagaagca actccttcgg aaataagctg aaattcacaa aaatttgaaa agcaattttc
      961 gtgaatttct tcttatttct tggagttaaa cgcttctgtc ccagcctcta ttttttatag
     1021 taattccttc agatactttc ttgcctcatc attgaaacgc agtgattctt ctaacttctg
     1081 ataatattcc aacgcttgtt tctgagcttc agtaaaacga taatgttttc ctttccattt
     1141 gtcgattgtc tcttgcacat ttccagattg gatatctttc actactagtt gccatttttc
     1201 ttttgagact gtacggtctt tcttatcagc agattgtata gcttctcgtt ctttacgcaa
     1261 tacttttaaa aaaccatttc tgactatccg aggaatgctg tcgaagaacc cttctgtaaa
     1321 accatagcga caatattgct tccaaataaa tagatagatt tttggttgga tgaatcgttg
     1381 gtttttcgca aaatattcca tctgagcagt gacttctggt gttcgttgac gttttgtctt
     1441 tctcaacacg gaaataaatg tttcaaaata agcattagga tgtactggca cttcttcata
     1501 tacgatggat tgccaatctt tacgtaaaaa tgcgcgatag ttgatcaaga attcttctac
     1561 atcctttact aattcttctg caaagtcgtc aaatagttct ttttttatct gtcgcttttt
     1621 tgactgccca ttgatgtgtt cacttatttt cttgatttca ttttccaatg cttgataatc
     1681 cgctatgtaa atcggcattt ctacttggcg aaattctgcg agccgctgag ctaattttat
     1741 atccggtaaa tatggctgtt tcatccgacg attttggtac aagacaaaac agtatttttg
     1801 ccatagtcct tcaggaaaat cgatattctt ctgcttaagc cataataatt cgtctaaggc
     1861 cagatcaact tttgtcattc cctgatggat cgaagctgcc actgtcggag agacatgcag
     1921 gtgatcggca aagtggttga tgcctagttt taggatcttt ccggtttttg gcgatttaac
     1981 gatgtattgc acttttaatt ctctaccaca ctcacaatac aatttttgct ggcttcgttt
     2041 tgttcgaaaa aacggattga tcttgaagtc agaaaacacc caatctgttg cagctaaata
     2101 atgttcttcc ataaaacgag agctgattag atatcggcgg tgcttatcga ctagttcccg
     2161 ctgttttttc gttaattttg catagatctc ccaacgttga ctgcttgtgt attgtttaga
     2221 aattttgatg ggatagcttt tgctgatttc ttttggaaac cattcttcaa agacggattg
     2281 ccattcttcc acggctgttt ctttcctttc taattacaaa aagctggcta ctaacataac
     2341 gttagcaacc agccagtgtt tcttgctaaa ttagtgagtt gttccggcat ttccggaaat
     2401 atcttcttta ttttctactg ctgcttcaac tgcataagtc aaagaagaag caatgtcttc
     2461 taaagacata gaaggcgtac ccactggacg atcgatcact tgatcgggtg agaatggcac
     2521 gtggatgaac ccacctcgaa ttgccgggaa ttgtgtttcg atcaaatgaa gcaaacgata
     2581 catgatatca ttgcagacaa atgttcctgc tgtataagag atgtacgctg gtagaccatg
     2641 atcttgtaca ttttttgtca ttgctttgat tggcaatgag gtaaagtaag cggctggacc
     2701 gttttcttct aaagccgtat cgaatggctg atttccttca ttatcaggga ttcttgcttc
     2761 tgctagattg attgcgacac gttcgaacga gactgctgac cgcccacctg cttgtccgac
     2821 acagatcacg acatctggtt ggtgctgttg gatggcttct ttgactactt gactgctttt
     2881 ttcgaatacg gtcggtactt ccactttgat gatttcagca ccgctgattt catctggcat
     2941 tttttttact gcttcatagg ctggattcac tttgtcccca ccaaatggat caaaacctgt
     3001 tactagtact ttcatctgtt gttcctccgt ccgattgatt taacatatgg ttatctttta
     3061 tcataccatt ttccttgaac agcgtaaata aaggaaacaa tagtttaaat aacataacag
     3121 atcaaactta atatattctt ataaaaaaat agtgttccac tacaatgtta gaagaggttg
     3181 cgacaattat tctgtcacaa cccctctctt ctattattta aatagaatca tataagcaat
     3241 ttggaaaact agcataaata acgcaataaa caactggttt ttgatcactc cgtatttgtc
     3301 tttcatctcc agcatcgcta caggcacgat attgaagtta gcagccatcg gtgtcagcag
     3361 ggtcccacaa aaaccgcaag tcaatgcaac cattcctatt aacgctggat tcgctccatg
     3421 actaaatacg aatggtgcac cgatcccaac cgtcatcacg gtgatggccg caaatgcatt
     3481 ccccatgatt accgtaaata tcaccatgcc aagagcgtaa atgatgatgc cgatgttcac
     3541 atttccttct ggaacgatcg tacctactgc attggaaatc actgtcccaa ctccagcgct
     3601 tgtaaatacc gcacctagag aagctaaaag cattggcaac atgcttaacg gccctactgt
     3661 gcccagcata tctgctgcat catctaaaaa tgtacttggt cgattatctc gagaaaagaa
     3721 catcaagatt agcatggctg caaaaacacc gacgcccacg ccgactaaag cacctaaatt
     3781 ggtaaataga gcaaacaaga tcgcaaagac accgatactc aaagctggaa taaaaatttt
     3841 cattcctagt ttgtctgaca ttttttccat atattctttt gatggaagcc ggctttctcc
     3901 tttgctgact ttcttagcaa ttgctggtat ggtcatcgca aagatcaagc caccgttcac
     3961 gattgccgga agccatcttc ctcctgcaat aactacccct aagaccgtcc agaaaaaggc
     4021 tgacccataa cgatgaggat tggttttatc tgttaaattt cttactccag tgtaaatcaa
     4081 tacgagtccc atcacgatat aaaggatctc cagtaattta tcgcctaata aaatatcagg
     4141 atttgtaaaa aagtccatct tattgcccct ttcctggata atatttcttc gctaatttct
     4201 tttctttaag atagaagtag acacttgcca caactaaagc cgtgatagcc actggaactt
     4261 ccactaaagc aagctgtccc aatgttacgt catatcctag atctttcagt gttgattgga
     4321 ctagcaacgc ccctgctcct ccgatgaata gtacttggcc aaagaaccag cagacatttt
     4381 ccatagctga agccatacct tttagttctt cttcatgctt aggatggact tctaaccctt
     4441 ttgattcgat ggtccccaat gccatcggta aaatgattgg acgaacaaat ccagcaactc
     4501 caccaaaact cacgttaaaa gccgcaaaga tcattcgaaa gatcccataa atatctacaa
     4561 tcataccagc agatactttc ttgaatcgct tgatcaagga agccgctgat tctttcagac
     4621 catttctttc aagcgttcct gttgccaaca taatgatgat aaaaatcgcc atcccgcgat
     4681 tgtttacaaa acttgtacct aaaagttcta acattccttg aatgccaaga ccgccagtca
     4741 aagctgtaac aactaaagca accattacga tcaatatcga gtctaattta aatgcaaacc
     4801 cgagaataac aatcaagacg cctaaaaggc taagataatt ttccatatat tttcctccat
     4861 aaaatgacaa tattttttta attataaagc aaaacacata aaaaatatag ttgttttctc
     4921 acgaaataga cactattttt aaatattttt cgttattttt ttatcacaaa aattatctta
     4981 cttattgtaa ggtaaagctt tttttaattt attgcccgat aatatataga aataaggtct
     5041 agttactgtt ttttttaaat gattaataaa taacaacttc tttctatgaa attgcaaagc
     5101 attcatcttc ttttccgcta acgatatatc cgtcagtgct tggtacactt tactaaaaag
     5161 tatgagattg ccgatatctg gataggtgtg ctatactaca gaaaaagata tacaatctgc
     5221 caccgggtag gataaagagg atgatcattc cattaggtct ttgaagggat gattttcctc
     5281 tttttgtgta tttttttata gttaaggagg aatgtaagta tggcatggat ggaattaatc
     5341 atagcaggaa ttttggaagt attctggtca acgatgatga aatggagtga tggtttttct
     5401 aaaatcaatt attcttttta cacagtgatt ggcatgattg ctagttttta ctttttgtca
     5461 aaggcaatca aaagtttgcc gatgagtctc tcttatccta tctggacagg gattggcgca
     5521 gttggttcgg tcataattgg tgtggtcttg tttcatgata agctgaatct ttccacttgg
     5581 ttctttgtgg ggatgctact catcagtatc attggcatca agattactag cggccactag
     5641 acacttggaa taagtactga tcagaaaaaa taagcctttt tatttaatat tcaatttttt
     5701 tatgaaaaaa taagaaataa gtccaatgac ctagcgactt ctccactcat caggtgtatg
     5761 cttgtactat ctatagaaaa ggagtgaata tttttgaaaa gtctttcggt ccaagaatta
     5821 tccaagaact ttggtcacaa acaagccctc agcgatgtca gttttagctt atcccctggc
     5881 aaaatcgttg gattagtagg tcctaatggc gctggaaaaa caacgatcat gaaagctatt
     5941 ttaggactga tccattactc agatggaaca atctgtattg gtgaaaatga gatttctccc
     6001 tctagtcata aagggttgga acaagtagga gcattgatcg aatacccggg tatctatcca
     6061 ttcttaacag gctatgatca tctgaagtta ttctctgaaa ccaacgatgt atctgctatc
     6121 gacactatcg tgaatcagct caagatggaa aagtacattc acaaaaaagc gaaatcctac
     6181 tcgttgggaa tgaaacaaaa attgggaatt gctcttgcct tactgaacca tccgtctatc
     6241 gttattttag atgagccgat gaacggtttg gatccacaag caacaagaga tgtgcgaaaa
     6301 atcattaccg acttggctac tcagggcacc tctttcttga tctccagtca cttactcagc
     6361 gagcttgaaa aaatggtcga tgagctgatc ctgatcaatc aaggaaagat catcaaacaa
     6421 tgtacaatgg ctgaattgaa ccaaacagcg caagatttca tcgttatctc tacaacaaat
     6481 gatctagctg ctcgaacgat cttgacagaa gctggatata ctttggaaga tcctgataaa
     6541 atcaagcttt taaaagaaaa cgaagaatta ttaggaaccg tgatccagct attgtctgca
     6601 caaaatatcc atgtgacaga cgtccagcat ctgacgaatg atttggaatc ttctctattg
     6661 gaaattctgg attcagtaaa gggggaagaa gaaaaatgac attgcttgta aaacaagaat
     6721 tatttaaatt gatcaagaaa aagtcaactg ctgttttatc tgttctatta gttgttttgc
     6781 tcataggaac agcacttcta gccaaaaaat acacgacgat cattgatcca gttgaaatga
     6841 ctgctcagct ttttagtgcc acatcttgga ttgtctttat catgattgca gctgcaagca
     6901 cgatcatttc gatggaagca caatatggta cattgaaaaa tctcctttat cgaaagtatt
     6961 ctcgaggaga aatattagta agcaaatgga tcacattagt tatttattct gtttatttat
     7021 accttttagc tattattgtc actgtgttga tgaaactgat tcttttccct tcgatttcct
     7081 tcacggagaa ggtttctact ggtcaaacac tgatccaaag cttgttcact tatactttgg
     7141 gtagttatat cggtttatgg ctgattttaa gtttagtgtt gatgattgct tgctttatca
     7201 atagttccgg cgcatccatc tcagcaggaa tcgtctttta ttttgcttcg tcaatcatat
     7261 ctggtatcct tattgcactc atccaaaaat gggagtggat caaatggaat ccgatcagta
     7321 tgttgaatct gcaaaaccaa attggaaacg aagagatcat gaagcaattg actcatcttt
     7381 ctactaatca aatgctattc ggtaatttag cttacatcgt tttgttctta gcattaggat
     7441 atctcgtatt taagcgaaaa aatgtatgat gactcatcaa aaaagaggct gtgaaagaga
     7501 cgttcagcct tattgccgat tggctgtctc ttaaacaccg tttattctga aaaaggagct
     7561 gtgacaacat ttgtgtcaca gctcctttcc tagtatgttt ttgaataaac gacaggctca
     7621 caacctctct ttatacttct tttatacttc tatacgtgaa gcatcccagt ggcaaataaa
     7681 accattgcaa tcactccgac catcgtcaca cctataagta aaatcttatc taagtggata
     7741 tgttgtacag gctcgttttg ctccttacgt gctttgaggt aaaaataaat gcctggcaag
     7801 aaaagaacaa gaacaagcat tacttcatta aaggctacga ctagcatagc ccatacttga
     7861 aaggcacttg ctaaaagccc tactaaaaat tggacccatt ctttccgctg ataagaaagt
     7921 ttcatttggt acaatcctaa gaaaagccac gtgaagaaaa tagctgctgt accaagtgtg
     7981 taagcaaagt tataagcact gtcagtgaat aataatgaaa gtaaaaagac attcgtacat
     8041 aacgtcgtca atacgatagc ataagttggt gccccatttt tgttgagttt tccccatgct
     8101 ttaggcaaaa gtccgtcttc agccatgttt cccattgttt caatcggtaa gatcgtccat
     8161 gagagccaaa caccaactaa agaaatgatc aaaccgccgt tgatcacgac tgctccccat
     8221 ttaccaacca tcatttcaaa cacatagccc atagcaggct gcgccttgat cgtttgcagc
     8281 tgattcatcg tcattgtccc atatggaagc atggaaacca agacgtaaat aataatcaag
     8341 ccgaatatcc ctagaatcgt tgctttccca acatctgatt gtttttttgc tcgtgaacct
     8401 acgacagagg ctccttcaat accaacaaat acccaaagca tcaccatgat gcaactcaat
     8461 acttgactgg aaaacgggat cggctgtcca ttcgcattca atataatgtc gctagaaaag
     8521 ttatcaatga attgttggat cgaaaacgtg ctgatagcta ctacaataaa taaaaataat
     8581 ggcactaatt tacagaatgt gacgatagca ttgacgaaac tagcattttc gactccccga
     8641 ttcactagcc atgcatatcc ccataaaaag actgtactca tcacgatcga tgccagattt
     8701 tgcccacctt caaatactgg gaagaaatat cctaatgcgc tcatcaacaa tgttgcaaaa
     8761 gctacgttgc ctaaccagca tgtgagccag taaccccaac cgctgataaa accagcgaaa
     8821 cggccaaaac cttcttctgc gtaactaaag atcccgcctt ttaaatctgg acgtttttta
     8881 ttgatattat tgatcgacaa ggagagcatc aacattgctg ttcctacgag tacccaactg
     8941 aaaatagctg ggccgccagc cgatccggag atgtccgtca taataccaaa tacgccacct
     9001 ccaatcgctg aaccaattac taaggcagca agtggaatta aaccaattcc ttttttctgt
     9061 tgctctgtat ccatgtaagc atcctccaaa ataaaaccgc ttttatcaaa aagtataaca
     9121 aacctttttt atttttactt aattaataaa aactttaata gaaatataat tctaccggct
     9181 tttttctcat tatacaagca aagcgcctag tccctttcca aattacacca ccaaaaaact
     9241 taaaaaagat gctatactaa aaggaaagaa ttggaggatg atccatgaac attttatata
     9301 tatcgatttc aggaaatacg cgtgcctttg ccaaacatct agcagaatat gctgaaaaaa
     9361 tgcatgcaga agatcctgtc aatcctgaag ttactttaaa agaaatccat gaaaatagtg
     9421 attttacgaa agaaacagaa ccatttttta ctttcgttcc tacttattta gacggcggaa
     9481 atggtttaga taacggtgat acagaaatct tgaccgaaac gatgcgtgaa tatctggaac
     9541 atgaagacaa tcataaactt tgtcttggtg tagtcggtag cggaaataaa aacttcaata
     9601 atcagtattg tctaacagcc aaacaatatg cacaaacatt tggctttcct ttcttggcag
     9661 attatgaatt acggggaaca ccatctgatg tgacacgaat ttatcagatt ttagcagaaa
     9721 acaataaata ataaaaaaga agaagctgaa aaatagattt cagtttcttc tttcgtttct
     9781 attgtttcta tcatattaag ctttgacatc catcacaggt gcttttgctt ttgctttacc
     9841 tgttttttcc aagacaaagt tttttacacg atccatattg gttaccaaga cgatcatgga
     9901 tgtttctttg cctgctgctt tgatcgcttc taagtcagct tctgctacaa gcgtatcagg
     9961 tgtcacttgc tgcccttctg ttactttcac atcaaatggc gtaccgccta agtcaactgt
    10021 gttgattccc atatgaagca agatttctaa accatttggc gttgtgatcc caatagcgtg
    10081 tttcgttggg aagacagagc tgactacccc agtaactgga gaatagatat gtccgttttc
    10141 tggttctaca aagtagcctt cacccatcat cttttgtgca aaaactggat cacttgcttt
    10201 atctaaagaa acaactgttc cgtcagcaac tgatgagagg atagtcacac tttcagctga
    10261 agtttgaggt actacttttt caggcacttc atttactgtg gctgtttcag tcgctaattc
    10321 ttctttttct aaacggtctt taggaatacc gaagaagaat gtagcaacga agccaccagc
    10381 ataagctgcc aataatccaa ggatgtagcc taaccattta ccgtcagaga tcaatgggat
    10441 caaagcggct cctgaaggtc caatagcgat agccccgaca tttccgatca ttccgatcac
    10501 tgccccgccg atacctccac cgatacaagc agtaataaat gggcgtccta gaggtaatgt
    10561 tactccgtaa atcaatggtt cgccgattcc taggatcccg actggtaaag ctcctttgat
    10621 catttctgtt aattctttat cttttttgca gcgaatccat aatgccaatg ctgcacccac
    10681 ttgtcctgca ccagccatcg caagaattgg aagcaatagc gtacgaccag tttcatcaat
    10741 catttgcaca tggattggag tcaaaatttg atgcagtccg aacataacca tcggcaagaa
    10801 taatgtgcct agaacgaagc cggcaaatgc tccaccaacg ccaagtaccc agttgatccc
    10861 accgaccatg ccatcagaaa tgaaacctgc taaaggcatg atgaagaaaa tttcaatcag
    10921 accgatggca atcaatgaaa gagttggtgt aacgatgata tctaccgcat caggtactac
    10981 tttatgcagt tttttctcaa cgattgacaa tagccaaaca gcgaagatga cacctaggat
    11041 cccaccttgt ccagctgcaa gagcttcacc agtaaataag tttttcagtg gtgcttccgg
    11101 attcatccct gtcagcatca caacggcccc gataactcca ccaagagttg gtgttgcacc
    11161 aaaaacttgt gcagcattga tacctgtata gataactagg tagctaaaca tcccgttttt
    11221 caagatgttc atcacatcga tatattgttg ccatgttgct gcatcaagtg ttccggctgt
    11281 gactaagttt gctaaaactg cggcaatccc tgccacgatc ccagtaccga caaatgcagg
    11341 aatcattggc acaaagatat tagaaatgtc ttttaatacc gatttgattt tagatggttt
    11401 ttgtttcgat ttttgtgccg ctttcatttc ttgtgcccgt tcgtttacac ggtcttttcc
    11461 agatgctgat gaatttgctg tagctgtact tacagaagaa agtttttctc ctaatttcac
    11521 gcctgccaaa tcaaccattt gctgtgctac tttgttcact ttcccaggtc cgatgatgac
    11581 ttgaagctgt tcatcttcta ctacaccaag cactcctgga atcgctttta attcagagac
    11641 atttacaaga tcatcattga tgatcttcat acgcacacga gtcatgcagt ggacaatgct
    11701 ttctacattg cccatgccgc ctgccccttt ataaatttgt tttgccagtt cttccaaagt
    11761 cagttcgttt tttgccatgg tcttttcccc ctcataaggt cccttttaca aatccgtttg
    11821 cttcagctaa ttttgcttga gcttcctctt tatcactatt tgtcaaaatc atgacgatag
    11881 ccagcttaac attttgatct gcttcttcaa agtacttttc agctgtttcg taatcagctt
    11941 ctgtcgcttg catgatgatt cgttttgagc gttcgattag tttttcattt gttggtttga
    12001 catcgaccat caagttattg aatactttac ctataccaat catactgata gtagaaatca
    12061 tattcaagat aagtttttgt gctgttcctg acttcaatcg agtagagcct gtcaaaaatt
    12121 ctggacctgc atcgacttcg attgggaaat ccgcgtgttt actgatttcg gcttgtttat
    12181 tgcaagaaat cgttcctgtg acagcaccga tcgattttgc gtaatctaat ccaccgatca
    12241 catatggtgt acgaccgcta gctgcgatac caataactat atcatttttt gtcaaattca
    12301 gcttgattag atcttcttgc cctagctcat aagaatcttc ggccccttcg acagctacag
    12361 tcattgcttc ttctccacca gcaattagac ctatgaccat ttccggttct acaccgaatg
    12421 tcggtacgca ttcagcagca tctaacacac ctagtcttcc acttgttccg gctcccatat
    12481 aaatcagacg tccacctttt ttaaaggact caatcgtgtt agcaatcact ggttcgatac
    12541 ttgtcagttc tttttcaacc gcagcggcca ctttctgatc ttctttgttc attagttgaa
    12601 cggcttgaga aatactcatc tcatctaatc caaaagtttc ttggtttctg cgttccgttg
    12661 ttaggttctc taaattgatt ttgctcattt ttatcttcct tcttttctta taaattgata
    12721 gttcatacca gctttgattt ggttgatcaa tggtagctct tctttgataa ctttagctac
    12781 tacattcact ttttcatccg ctggcaaatc atatttggtt aattggatct cacccatata
    12841 gcgcaaatac ttttcgttat ctaaagtaat agaccctttc atacgcgtag ctgtatttct
    12901 tgctgggata ggcggaatct ctcgaaaacg tgcatctacg cttcgtatga catctcttgc
    12961 atcgtcttgc cgattcgtat gcttccctaa tatatattcg taaaattctt catctacagg
    13021 ttccgtatgg agcaagactg tctgttcttt ttggaaagaa gcaaattgct cctgaacgtt
    13081 ttctgacaga cctgcatctc cgatgtaaac aatatccgtg ttacttgcta atagatctag
    13141 agcgcctgca agaggatgga tccctcgatg ttcttccaaa gttggcaatc cttgatatag
    13201 tgggcctctc aactctgtat ctcctggtac aaaaccttgg acaaggagac cttgaatttt
    13261 gagccattga ttttttttct gataccattc tttgtccagc cctgtttcgg gacgtggata
    13321 ataattatgc caagcttcaa tctgtgagaa atcagcttct gcttctttca attcatcgat
    13381 atctttaggc gtgatcgtgc tagcattcaa actgattttc aattgatgtg accagtcggc
    13441 aatctgctga ttggaaatat gataatccat tcttaaacct gttacaccaa ttgctttcaa
    13501 aggcgttaga tcttctaatg aaaaacccgc tcgttccaat gcctctcctg aaatatcgac
    13561 catcaattct agtttattct ttttcgcttc gcttcctagt tcgatcaatc gcttttgata
    13621 tgccgaaaca tcatcttctg gtatatgcat ggaagtgaaa ataccgacaa agccattgga
    13681 tgccatttgg cggatatact gtttcttctc ttctgacaaa gaatcgttca taaatattga
    13741 aaagccaaac atattatccc tctttttctg tatccgtctt catttctatg actagagtat
    13801 atatctaaag aaacaaaatt tcaatacttt tattaaaata attaaatttc atttctaaag
    13861 gttgagttta tattatcaaa agaattgtca taacctaaaa aaggcactgc taagcaaaag
    13921 cagtgccttg aaatatttta tttaaataac acaaaaacat aattactcat aagaaaccaa
    13981 ttctgtatta tctaccatga atgtaaagaa cccctcatta ggcatcggat gaaaataagc
    14041 attgatcact cttatcaagg cattatggca aactaacaga tagacatttt cttcgtcttc
    14101 gatgcattct ttcaataaag gaacgatccg tgcatacaca tctaagactg actccccatt
    14161 tgggaatcgt acagcgaacg ctaaacgtgc tttttgaaag ttctcatctt tgcttggcag
    14221 tccatcataa tccccaaaat ccatttctac caatcgttca tcaacggtca gcggcaagct
    14281 gagacgatca gcaactgctt gtgctgtatc tcttgctcgt tgaagagggg aatggatgat
    14341 tttcgtgatt ggaacaggca aatctactac cttttctgct agtttctgtg cctgcatctg
    14401 tcctttttca gtcagcggca catctgcccg tccgcaaact aatccttgtg cgttccaagt
    14461 tgtttctccg tgtctcgtta cataaagcat gtgattctct ccttcgttgg caaagttccc
    14521 cttaaccata ccttgttgtt cttgagatta caagggttag attctctcaa gagaaataat
    14581 ctcagtagat actttatcca ggaaggtttg atcatgttcc actagtaaca tggcaggttt
    14641 cacgttcaag atcagttgtt ctaattgttc ttgattgaag acatccaaat aattcaatgg
    14701 ttcatcccat gtatatagtt cagctggctg tgataaagat ttagccaatt ccactttttt
    14761 ccgttggccc atactcatct gctcgatctt gttatgaaaa acatctcttt ccatcccaag
    14821 ctttcggagg ttgttcaaaa atgcttggta gtctacttgg tttttctctg caaattccgc
    14881 caacgttcct cgattgtctt cataattttg gcttgcataa ctaatgctca gatgttttgg
    14941 ctggtatttt tctcctataa ccttgccgtt gaatgccccc agaagataat ggataatgga
    15001 tgactttcct gcaccgttag gacctgaaat cgccacccgc tgatgaggct cgattgtaaa
    15061 atgaattggc tcgaataaca gattttcata ccctaattga agatcttcta cgcttaaaag
    15121 tcgcttatgg tgagacgctt ggctattcat cgtcaaagaa tcgatatatt cgatatcttt
    15181 taatagtttt tctttctcgc tgatctgggt ctccatccga ttcacgattg ctttggatcg
    15241 tttcatcgtc cgtgcagcat cagcacccac tgctccttta ttcactcgtc tagattcagt
    15301 atcgatgaat ccgacttgtt tctttgtttt atctccttct cgggaacgag accattcggc
    15361 tttttcagct gctgttttct ttagcctact gacttctttt ttcaattttt cattttgagc
    15421 catttcaaat tcatcacgaa gttttttctg ttcttcatag atagagaaat tcccttgata
    15481 aagttccagt tgactttttt cgattgctaa aacatggtcc actacttcat cgataaatcc
    15541 ccggtcatgg ctgaccacga tgaagccttg ttttttcttt ttcaaataag ccgctacttg
    15601 ttttctacca gagatatcca aatgattcgt tggttcatcg attaacggga aatgagtgtc
    15661 atccacaaat aaaagtgcca gtaggacttt tgtcttctct ccccccgata gtgtgctgaa
    15721 ttctctccat aagatttcag gatctgtttg catcaattgg agctctcttt cgatttccca
    15781 tatctcaaaa tccgtaatat catttaacac gtaataggtt aaacgttctt tatcttttgt
    15841 ctgttgcggg aaataggcaa attcttgctg atggattact tgcccttgat aaggtagttt
    15901 gttttgtaga atattcagta aagttgtctt tcctcgaccg tttcgtccga taagtcctag
    15961 tttccattgt gtgtcaaaat ttagatttgc ttgttcaaat aataatgtgc cttggctgtc
    16021 gtagccgaat gtcagatttt ttatttcgat tttagacatt ttccatcacc tcgtaaaaaa
    16081 agaccagtcc tattttcaga actggctaaa gaggatgcaa gggttggata cacacaaaaa
    16141 gacccataca tcgaaaaaac caagccaatc ccgaaaatct gcacactgct tccttgtctc
    16201 cacaaggtac agtctgaatg cagctttcat caatcgactt aatttttcaa tgcacggatc
    16261 cctcactttg tcctatattt gaaattacga tagcataaag aaaatgattt tgtcaactac
    16321 ttcgggagga tggtgaaaaa tctgctaagt gtctgtccta agatttttgt tttttctaat
    16381 gtttgcctgt gttccattgc ttcttctaaa cctattggac ccaaatgaat acttaagaca
    16441 attgggagaa aatcagaaag tccttctagc tcttggtctc tacttccgca aatagcgatt
    16501 attggttttc gatatttttt tgcaagcact gctacaccgt atggaacttt tccgtggatc
    16561 gtttgctgat cgatccgccc ttctcctgta atgatgacat ctgcttgttc gatctttttt
    16621 tccaatccag tgatttggct gactaaatcg aaaccagaaa ccatcttcgc tcccaacaaa
    16681 aaagggctcc accaataccg cctgctgctc cacttcctgg aatatcatct aaatccacct
    16741 cccagaaatc ttttatttgc tgagcaatct ttcctgcttg tttatctaag aaagctagcg
    16801 tcgtatctat tcctcctttt tgtcttccaa attgccaagc agctccttgt tgacctgtat
    16861 atggattggt aacatctgca gcgataatca attttacttt agaaacaaga acgtttcttc
    16921 gatcaaaatg aagtttttta gtagataata aaggatttcc ttctgtgtac ccttctaaag
    16981 aagcacccaa ggattggagt aatcctagac caccatcgct acacccgctt cctcctaaag
    17041 tgacaataat ttgttctact tcttgaagca gtgcatcttt aataagttct cctaaggcat
    17101 aactggaagc aagtcggata gtttgatccg ttggtttatc aagtagatct aaccccaatg
    17161 cttgtgcaga ttcgatgact gcggtgtttt ttccttgcca agttgtcaat aaatagctga
    17221 tttttcgctg acggaataag agatccatcc catcaatggt acgccatgtt cccctgagcg
    17281 cgtaatagat tgcttctgca gtattttctc cgccatctgc aatcggcaca ttgatttttc
    17341 ttcctttacg atcttcccag ccatttagaa ctgcctgatt tagttcaaat gaagaagcac
    17401 ttcctttaaa tgaatcaatt gccgccaaaa tatccatctg tatccctcac tttcttctta
    17461 agtaagacac tttttttcat ttttttcaaa caaaacaatg ctattttctt ccatttttct
    17521 atatactaaa agggaactca ttgaataaag gagagaaaaa acatgaaaat cgcaatagct
    17581 ggggccggag caatgggcag ccgaatcgga ttgatgctac atcaaagcgg aaatgaggtc
    17641 ctcttgatcg atcgttggcc tgctcacatt gaagccattc gtacaaatgg gttgattgct
    17701 gactttaacg gtaaagaagt ggtcgcaaaa ctgccaatct attcaccaga agaaatcatc
    17761 gaaagcaacg aacacgtgga cttgatcgta gcattaacaa aagccaatca attagatgat
    17821 atgttttgtt cgatccaatc cattattact gacaatactt atgttttgtg tttactaaat
    17881 ggattaggtc atgaagatgt actggaaaaa tatgtgccaa agaaaaatat cttatttggg
    17941 atcacgatgt ggacagctgg tctagctggt cccggaaaag tcacattgtt aggtgatgga
    18001 gaaatcgaac tagagaactt ggaaccagaa ggcgaagcat ttacaaaaaa agttgtagaa
    18061 gtctttcaag aagcaaattt aaatccgatc tatagccata acgttcgtta ttccatttgg
    18121 cgaaaagctt gtgtaaatgg aacattgaat ggtttatgca cgattttgga ttgtaatatt
    18181 gctgaactag gcgcgcaaaa agcggcagag agcatggttc gaacaatcgt atctgaattt
    18241 gcttctatcg cagcaaaaga aggaatcata ctagatcaag aagaagtgta ccaacatatt
    18301 gcttctactt atgatcctga caacatcggg ttgcattacc catctatgta ccaagacctg
    18361 atcaaaaacc atcgtttgac tgaaatcgac tacatcaatg gtgccatttg gcgaaaagga
    18421 caaaaatatg atattgctac accatattgt gcctttttga cccaattagt ccatgcaaaa
    18481 gaaggaatcc ttggcgcaga ataaaaggct ctctgatgag aatcagctga atcagcagct
    18541 ggttctcttt ttttaagccc aataaaagat caaaaagaaa acacaactct catgatgcca
    18601 taagaattgt gcttgtttgt ttcagtacat aatctcttca aaatgtaggt tttttatttg
    18661 ttttcaacag aggagaggtc atattcgtat tgctcataaa tagtggaatc gttagtgata
    18721 aatcgttttg cccaattttc tgcatcataa aattccaata gctctgatag gattggctta
    18781 aatggtgtat gcagaaattc cggtaattca tctaaatcac gttctcgaag caaaaaaata
    18841 ttttcaggac gataaaaatg ataatgagca atcgcttgat tatccgtcac tagtttcttt
    18901 tgaaaataca tggattcaaa tgtccgcaaa gtcactcctt gctgaccctc ccgcaacagt
    18961 tccagaatcc cgcgcgattc ccctgttttt ttcaggtact cttcataagt aagatatcca
    19021 ggttgttcat tccctcgatg tggaagaatg aaatagttgg tcgtaacatc tctttttata
    19081 aattcctgct tatagctttc tgctcgttct tttctcccat tattcgttgc accaaaaaat
    19141 agatccgtct ttggttcgct agaagaaaaa ttcatatgtt tcgaataaaa tgaactatta
    19201 tgtttcagcc caagttcttt tgcttccaaa aaatcaaaat gataaatctc atcgattgcc
    19261 ggttcatctt gtgcctgctt cagcatcgcc tcgtgttctg ctttgaagtg attccaaaaa
    19321 aataacacaa tttttgcatt ggtaaatggg cgcacgcgtc gagccatctc catatcaaat
    19381 ccataatcta aaaaaacgat cagctgataa tttgccaact gttgtctagc tgatttaatg
    19441 aattccgact gtagtaaatc ttttccggac caaacatcta aagagcggat ggaaggcaaa
    19501 aaataaagtg atttattttt tgcaactagc agtacgtcat ttttgttcat cttttccgcc
    19561 tcactttctc tgtcactttt tcctatgata gcctgtttaa atagtaaaaa gccagtaaaa
    19621 cgtttcctat atttgtatga aaatttaaca tttgtatgaa aatatgatga aataaatcat
    19681 tcttctatta aaattcgcct aaaagttttc tgaatagttg taattgtctg aaaatttgtt
    19741 atcattagtc attgttaaat attgctattt attcttaata taaattttga ttgtgaattt
    19801 aaaagaaaga ggtcatttat ggaagataaa aaattgaaaa aacaacttag ctccagacat
    19861 atcaccatgt tagctttagg tggagcaatc ggtgcaggtc tatttaaagg aagtggcgaa
    19921 gcgattggga tcgccggccc ttctgtattg attgcttttt tgattggtgg gctgatttta
    19981 tatatcgtga tgaaaggtct gggaaaaatc gttttaagcg gtggcgacac gcatcacgga
    20041 ttatctggtt tgatacgccc ttatctaggt gctcattctg ctgattttac tgattgggtc
    20101 tactggtcta tgtggattat caatatcatc gctgaagctg ttgcagctgc ttcctttttg
    20161 cagttgtggt tcccgcacat tccagcctgg gtatttgttt ttatgttggc tgttttaact
    20221 acgatcatca atctttattc ggtacggtta ttcgcagaaa cagaatattg gctggctttt
    20281 gctaaaatca gtgtcattat cttactgatc atttttgcta cttatcttgt cggtcagcaa
    20341 atgttgggca caggcgtttt ccctaccctt cagcaattga ctgaccatgg tgggtttact
    20401 cctcatggta tgaagggaat catcagttct ttgcttgtag ttatctattc ttacggcggt
    20461 tctgaactga ttgctatcac ggtaagcgaa gcagatgatc ctaaacgtgc gattcctaaa
    20521 gcaatcaaag gtgtcatggg aagaatcatt tccttttaca tcattccttt gtttttgctt
    20581 ttgattcttt ttccttggaa tactttagca ggcacgaatg tcagtccttt tgtgatggta
    20641 tttgaaaaaa tgaatatccc atttgcagcg gatatcgtca attttgtgat tgtccttgct
    20701 ttgttctcat ctatcaattc gggtgtgtat gcatcttcaa gaattttgtt cttccgtttg
    20761 aaggatcgaa aaggttcctc tagaaaacta gctgttttaa acaagcatca agtaccgcaa
    20821 cgtgctgttt tattttgtac cagtgttctt tatcttggtg tagctctttc ttattttgtc
    20881 ggtgacaaat tatttggcta tctggcaggt tccctttcgt atacagtatt attgatctgg
    20941 atcattatca gtgcggcatc ctttgtactg gcactaagaa aaggaacatt atgggataaa
    21001 ggaatcagct tttttgcatt agctgtctta ggattgattt tccttggtat cctgttcacg
    21061 aactccattg gggtaactgt attgactggc ctgctttacc tgttcattta tttcagttat
    21121 caaaaaaaga atgatgcgtt cgtattgacg aatgaatgaa atcgatagaa aattttattt
    21181 tatttatgtg gaatagatgg agctattgaa gaaaggagtt gtgacaaaag tcttgttgca
    21241 actccttttt ccgaataaac agtctcatga cctcttggta aacggtgttc aaaaaccctc
    21301 tgtgcaaaat aattgattgt tctccatgct catgacatga tagaagaaag gaatgaaaaa
    21361 aaggagtttg ttttaaatga aaaagtattg gaagtttttg ttgatgatcg gtgtttcaac
    21421 ggtagttatg tttggtatga tgtacttgaa tgtctatgcg ctcgatcata tcttcttcag
    21481 tcgtacacga gtattcatgg cgttgatgat gggtgcagtc atggcagtca tcatgctgtt
    21541 gttcatgtgg aaaatgtatg aaaacaaaaa gataaatgtt atgatcttag ccgctagtct
    21601 tcttgttttt gccggttcct tgtttatggt acgaagccaa actactgtca acgacacagc
    21661 ttggatgaag gccatgattc ctcatcattc tatcgctatc ctggcaagta aacgagctga
    21721 tttaaaagat cctgaagtaa aaaaattagc ggaagaaatc attcaggcac aagaagaaga
    21781 gatcgacaaa atgaaaaaaa tgatcaaaga aatggaatga ccattatccg tccaaataaa
    21841 aaaagaggat gttccagaag ttatcagctt caagaaataa gaagaagcta cttctggaac
    21901 gccgtgaata aggggttgtg aaaaagctgc cctgcatcaa gtaataagaa cagccaaaca
    21961 aaaatagctt ctcctatttt tcgtttggtt aggcttatta ccgcagatgc aagcttttga
    22021 acaccgttta ttaagaggtt gtaaaatcag cgttctgacc tgagtattag agagacaaat
    22081 ggaaaaatag ttcctcatat tttgccattt tttgagctaa taccgagggt cagctgattg
    22141 aacaccgttt atcgagaggt tgtgaaaaag tcgcttagtt tcgcgtatta agaaaaattt
    22201 tgtgaaatgg ctttccacat ttttgtataa atttggctta ataccgagaa actggctttt
    22261 gaataccgtt tattcgtaaa agggattgtg acaaaagtct tgtcacaatc cctttttatg
    22321 tttttattgt tctaattgcg ctgtcttgct tactgcttct tgcggtttca caaatttttt
    22381 tgagattccc caacctgtga aggcactgac catcgaaaac acaggagaaa gcaaactgaa
    22441 aaatacgaat ggcaagtaag tgagtgtagg aataccaaat gtacttgcta cgaaacttcc
    22501 ggcaattccc caaggcacta agtagttgat tactgttcca ccatcttcta gcgttctacc
    22561 taatacagtt ggatcaaatc ctgctttttc ataagtttct ttaaatgcat taccaggcag
    22621 aataacagat aggaattgtt caccaataaa aatatttact ccgatacttg tcatcaatgt
    22681 tgtaaaaatc aaatttggtg tgttcgtcat ctttcttgat actcgttcca tcacggttga
    22741 gatcaagcca aattcgatca acaatccacc taatgacaaa gttagaataa tcaatgacac
    22801 agtaggcatc atactttcga ttcctccgcg gctaagcagt gaatcgattt gttggtttcc
    22861 agtatttgaa acaaaccctg attcgatgac cccagccaac ttcgttaagg aagtttgtgg
    22921 gttttgaata aaaatcattc ctactgctac agcgatattc aacaaaatcg ttaaaattgc
    22981 cggcaccttt ttccatgcac acagtaacat taaaaataaa ggaatcagtg accagattga
    23041 aatagtaaag tttgcttcta gaacatcagt cacttcacga atagatgaaa gacttgcggc
    23101 tttacttgtg tgtcctaaaa tcatgaataa aaagaaagaa acaacaaatg ccgggacagt
    23161 tgaccacatc atatgcttga tgtgtttgaa caaatcggca tcaacaatag ctgctgctag
    23221 atttgttgat tcggataatg gagacatctt atctccaaaa acggcacctg aaacaatagc
    23281 tccaacaacc agcgctggat ttaagcctag tgttgtccca ataccgaaaa atgcaatccc
    23341 aatcgttgac ataacagtaa aggcactccc tacagcgcta cctacgattg cacacacaac
    23401 aaatacagaa gggacaaacc actggacact gatcaatttg aacccaatga ccattagagt
    23461 tggaatgatc cctgcttgta tccatacagc aatcaatgaa ccgactaaaa taaaaataaa
    23521 gattgggatg attcctggtt tgatgccatt gatgattcct tcgtgcagcc aatctgtcgg
    23581 gtatcttttt atcactgcat acaacatgac cattccgatc gctgctaaga tcgtgatatt
    23641 aggtgagacc tttaatccga caacgccaat cgcaatgcct aaaacaataa ccattaatac
    23701 aagtgttgct tctaaaaaac ttggtgttcc tttttttccc atgataatct ctcctttttt
    23761 agttgttctt atgttctaaa aaagttaaga tatcggatta ctccgccgca attcatccgc
    23821 gattcctcct ttataaaaat aaaaagacct gttttaaact aaggacgaat gactccgcgg
    23881 taccacctta attaaaaaca gatctgtttt tcactttcaa acttttattc aaatgtatgg
    23941 actttccatg taattcgtca attaggcttg atcggttcac agcgaccacc gactttctga
    24001 acaaaacacc taaccgctac tgcgtgaaaa gttgacttgt acgaagttta ccttccataa
    24061 gatcatttgt caatgaaaaa gaaaatattt tttcattttt ctgaaaatta tttttataac
    24121 tttcacacac aagtttattt tttactagaa agtagcgccc tcttgttttt ttcttgctac
    24181 tatttcttga tgttcttgct tgattctttc taatagcttt ggctctttca acagatcaag
    24241 agctgtattg gcgagtagtt ctgctgcaat cgcaatagaa gcaaggcctt tgtcactttt
    24301 agcagcagct ttgaattctt ctgtgtgtcc ggcaatatat gtctcagaga ttgctaccgt
    24361 aggctggatt gttggtatga cttggctgac atttcctaca tcagaagaac cgagacttgt
    24421 tctttgttcc gtttctattt cttcatctgg aacacctgct tcttttgcat gctggaagaa
    24481 taagtcatca aaagaaggag taacgatgac atcatcaact gcattttgga acaatccaaa
    24541 ctcaaaagtc gtgcccgtac ttatcgcagc acctcgtacg atattttcca ctttttcata
    24601 tactttatct aatgtttgcc gatttcctgc acgtaaatag aaacgtccag cagcgtaatc
    24661 tggaatgacg ttggcagcta ctcctccgtc tgtaatgatc ccatggatat tgacatcttt
    24721 tggcaattgc aagcgtagag cgttgattcc attaaacact tggatcaatg cttccaaagc
    24781 atttactcct ttttcaggtg ctgctgctgc atgggaagcg acaccgtaaa atttgatgtc
    24841 gactggatca ttcgctaaag attctgtcgt tttcccataa cgaaaagctg gatggacgca
    24901 taacgctaca tcgacatcct caaaaaaacc ttctcgaaca aagctgcctt tagctgatcc
    24961 gttctcgccc ccttcttccc ctggggtgcc atatacacgg atttctccac cagtttcatc
    25021 aatgaattgc ttcactatgc tggcggctaa tacggagtaa gtaccaaaca aattatgccc
    25081 gcatgcatga ccaatacctg gtaaagcatc gaattctgcc aaaaaggcaa ttgtgggacc
    25141 gggtttattg gaaatataac gtgcatcaaa ggctgttcgg tgtccagcta cttctttttc
    25201 tacccaaaac ctttcttttt ctaattgttt aatcaacgct gctgaggaat aaaactcata
    25261 gttacttact tcaggatgtt cgtggatatc tagagcaagt gcttgatatt ctggtaaacg
    25321 atattggatt agatccttca attggtttgt tgagattgtt gttgtcataa aaaattcctc
    25381 ctatgctagt catttttctt attttctccc gaatgtttcc catgcaggca cgcttgatcc
    25441 ttttgatgtt ttctcgatta ctttgactgt gtcttctgtt tgataagcgt ctactatttt
    25501 ttgatacgtt ttattgtttt catcttcttt tcttgcaacg atgatattca cataaggacg
    25561 tgcatggtca tccactggtt ctaagaagat ggcatcagaa gcaggattaa atccagcatc
    25621 tacagctaca ccgctattga tcactgaaat gtctacatct gacaaagcac gtgctgtttg
    25681 ggaagcatct aattcagaaa tgtctagatt ttttctattc tcggtaatat cagaaacagt
    25741 gggcgcttgt ccttttgcct tatccacttt gatcaatcca gcagtttgca ataacagaag
    25801 agctctgccg ccatttgtaa catcattagg gattgctact tttgctccgt ctttgatatc
    25861 agctacatcc ttcaaagtgt tagaataaat tcccagcggt gcattgacag tatttccgat
    25921 cgatactaaa tcagttccgt gttctttatt gtaattgtct aaaaagaatt gatgttggaa
    25981 agaattcaaa tcaatcgatc catctgctaa agccacattt ggttgattgt aatctgtaaa
    26041 tttcacgtat tccaaattaa tgttctcctt tttcaaacgg tctttcacgt tgtcccatac
    26101 atctgtatct tctccaataa tccctagttt tacgatagtt tcttttttgt cagttgtatc
    26161 tgcttttcct tggctacacc ctgccaacca aataattgct acaaatgtaa tgactacaaa
    26221 acgaataatt tttttcattt tttctcctcc actttcaatg tgtaattttt ttaatcagtt
    26281 gattactgat aaattgactt aaaaaaacta agattagaat caataatgta gccacaatcg
    26341 ttacatctgt ttggaatcga ttataccctc tcgatatagc aagattccct aatccgcctg
    26401 cacctactgc tccagccatg gtagtcagtc cgatcacgtt gatgatagtc aacgccgaga
    26461 cccgaatgat accaggcaaa ccttctaaca aatacacacg taaaatgata ccgattggac
    26521 tggttcccat cgcttctgcc gcttcgatca ccccaggatc aacttctagc agtgcgttct
    26581 cgatttgacg agcaaaaaac ggaacaactc caaaaatcaa tggcactaaa gctgctttcg
    26641 caccaatcgt cgttccaact aagaaacgag tagtcaatgc taaaagagaa agcaagataa
    26701 tgaaagggat ggaacgaatg atattgatca gcttatcaag tatttcaaat agcagacggt
    26761 tagctagtaa tccattaggg cgtgtcacta ctaacgtcac acctaagatg attccaatca
    26821 tcccgccaat gaatgctgtc caaaaagaca tatatagtgt ttgcttcgtc gcttcgacaa
    26881 attcatccgg aatcaaataa acattgggca aatatgtttt aaagaaatca gtcattttat
    26941 cttcctcctt cgatcacttt gatacgacta tctaaatcgt ttttttgtaa aatatttatt
    27001 tttacaccag atttgatcaa ataatccaat gcttttttcc gctgtttggt ttctccagct
    27061 aaagtaacga tcaaactccc taacggcgtt tcttgtaata actctacatt gccatataaa
    27121 atattcgctg ttacttgata tttgctgtac agatgagcga ttagaggttc gttcgtctga
    27181 tttccaatat aacttaattc caccagccat ttattggcga tctgatcggc aaaagcagaa
    27241 tgggcaatga tcgtttctaa tgcttggtca agatgagtag ctgttcgaat aaaatctttg
    27301 gtcaattctt cttcaggatt gctgaagatc tggatgctgc tacctttttc aacgattctt
    27361 ccatcttcca taaccgctac cttgttgcag atttctttga caacttgcat ctcatgagtg
    27421 atcaggacga ttgttaaacc taactggcga ttcaattttt ttaacaatgc aaggatctga
    27481 agggtcgttt tcggatcaag tgcactggtt gcttcatcac ataataatac cttaggttca
    27541 ttcgccaaag ctcgtgcgat tgctactcgt tgtttttgtc cccccgacag ttgtttagga
    27601 taagcagatg ctttttcttc caaaccgaca agttccagta gttcattcac tttttgacga
    27661 cgttcctgtt tcgattttcc agaatacttt aaagaaaaat caacgttgtc aaaaatagtt
    27721 cggctttcca ttagattgaa atgctgaaag atcataccga tggcctttct ttcttttcgc
    27781 aattctttgg gagagagctg actcagattc gtctgattga caatgacttc tcctgctgtt
    27841 ggtttctgca gcaaattcat cacacgaact aacgtacttt tccctgcacc tgagtaaccg
    27901 acgattccat aaacatcgcc tttttcaatc gttaaatcta cagaatctac tgcctgtatc
    27961 tctttttctt tttgtttgaa acatacagaa acatctcgca attcaatcat taaaattcct
    28021 ccttatctaa aaaaagcgtc ctcaagaaaa tgaaaatgtc attttcttga ggacgctttt
    28081 ttgcgtgtta ccacctcatg ttcaagctgt gttcacacac aactcctctt caagtacgat
    28141 catactctag caagatatcg gtcgcagccg tcatcgcctt acatctatgc tcgggatgaa
    28201 ttgctcgatg gccattttcc aatagtcgtt ccttgcttct tctcagctac cgaagctctc
    28261 tgtcaaaggt tcttctatcg tacttaccat ttcaatgcaa ataatattca aaaaaaaagt
    28321 ccttttacta acataaacta tgtcagtaaa aggacgacgt atcgcggtac cacctttttt
    28381 cggaagtttc cttccctcaa gcccaacatc tcaaaaagag agattggcgt tcgttttaac
    28441 ggtcgaacag ttccgaattt gcctacttat cgacaaatta ttctccaagg ccattttcag
    28501 cttttcttac ttgactcttc tcaccagcca gagcctctct gcaaagtttc caaacttact
    28561 ttccttttca acgaaatcga tattttgttg ttgggattaa tctacatgag gaaaactgaa
    28621 aagtcaagta cgaaattcaa attatttttt ctatatttat ttttctgttt ttttatttaa
    28681 catttcaata acagttcgaa gatcttttcc ttccgcaaga agtttcaatt tttccttttc
    28741 ttcgacggta taatttcgga caaaactgga atcaggtatc aaaacaacgc ctaatcctgc
    28801 tcgtgaagca gctgttgctc ctactaaaga atcttccact gcaaaggctg aactagcttc
    28861 taatcctgtt cgttccaaag ctgtcagata aggaatcggt gaaggcttgt tctccgacac
    28921 ttcgtctccg aatgtaaacg tgtccaagta atcagctaat ttaaagtgat ccaagatatc
    28981 tgttgctctt ttcgtaacag tggtcgtagc aatgcctagt ttcatatgac gttcttttaa
    29041 gtctgccaat atttcttttg catatggttt taagtggatg gctccgcttg tcagctgttg
    29101 ataaaaatat tcttcacgct tcgctcggac ttcctgtact ttttcagggc tacctgctgc
    29161 tttcactaaa atatcaaaac tttgtttcca gttttttccg ccccaagcgt caacgatttc
    29221 ttttgggata tggatatcaa attgctgaaa tccccaaagc cagccatcac gatacgtttt
    29281 ttctgtgtca actaacaaac catctaaatc aaatattgca cctttcatct atacattaat
    29341 cctttccaaa acaatacaca tagtttagca cgactggtcc aaaaagaaca gaagcaaaga
    29401 accagccttc tcgtttataa gagttcttgg aatcgacgga tatagatttt cttcacagct
    29461 gtcacaagcg ccagataagc gatgactgta gcaaataacc ataaccagta attgccagga
    29521 agtggcatca agcctaattc agcaccaaaa cttgtaaaag gaaggacaga accgacaata
    29581 attcctaatg tagtgataac aaacatggca aatgttgcgt tgctttggat aaatggtact
    29641 ttcggtgtac gcagtgcatg gagcactaag gtttgtgtcc atagtgattc gacaaaccat
    29701 cctgcatgga ataatgcgat gaaaatcact cgctgtgaac tatccaatgt atggaaactt
    29761 ccgcccacta cagcaggaca aataacaaaa tacatcaaca agtaagtagt aatatcaaat
    29821 atcgaacttg ttggaccaaa gtaaaccatg aattccccaa tactagatgc attccatttt
    29881 tttggctcgt ccaaatattc tttatccatc cgatcccatg gcaaggaaat gcaggaaaca
    29941 tcatagatca gattcaaaaa caaaagttgt aaaggcagca ttggtaaaaa tggcaggaac
    30001 gtacttgcaa tcagcacgga aaacatattg ccaaaattag aacttgctgt agccttgata
    30061 tatttcatga tattcccaaa agtttctcgt ccagaaagaa ggcctctttc caaaatcgtc
    30121 aaatcttttt ctaaaaggat cacgtctgca gattctttcg caatatccac agctgtatct
    30181 acagaaatac cgatatcagc cactttcatt gcaggagcgt cattgatacc atctcctaaa
    30241 aatccgacag tatgaccatt tttacgtaaa gctgtcgtca aacgtgcttt ttgctgagga
    30301 ttcaatttta cgaatacttc atggtcttca gctacttgtg ctaattcatg atcactcatt
    30361 tccgagattt tctcaccggt aaccaattca ttgatctcta gacctacttg acggcacact
    30421 gaacgtgtaa ctaattcgtt gtccccagtc agaactttta cagctacacc atgttctttc
    30481 agagctttca acgcttgttt cgttgtttct tttggtggat caaggaaagc aagataacca
    30541 atcaaaacca tgtctgactc atctttgact gaaaattctc caacgacgct tggattggtc
    30601 ttttgtgcga caccaatcac tcgtaagcca tcttcattaa gtgactgtac ttttgcaagt
    30661 acggcttctt ttcgttcttt tgttaatggt gtaacagtac cgccaagatc aataaattta
    30721 gaaatgttca gcatttcttc aatcgcgcct tttgtgatca tttgtgtttt accagctgga
    30781 tcagcaacca caacactcat acgacgacgt tcaaaatcaa aaggaatttc atccactttt
    30841 tgataattga tcttatctgt atctaaagtt tctgttgctt catcaatgat agccacatct
    30901 aacaaatttt tcagccctgt ttgatagtag ctatttagat acgcatggcg caaaacacga
    30961 tcatcttcat ttccttcaca atccatatga tattcaagaa tgattttatc ttgtgtcaat
    31021 gtccccgttt tatccgtaca taacacatcg attgcaccaa agttctggat ggcattcaaa
    31081 tttttgatca ctgttccttt tttggccatc acacttgctc ctttgaccaa attcgtcgta
    31141 acgatcattg gaagcatttc aggtgttaga ccaactgcta cagatagtcc aaaaagaaaa
    31201 gcttctagcc aatccccttt tgtaagccca ttgatgacga taactgctgg agccatcaat
    31261 gacataaatt tgatcagtag catggatgtt ttatggatgc ctaattcaaa gcttgattga
    31321 attggttttt cagatagcgt ttctgctaca tgaccaaata gtgtttcatt tccaacagaa
    31381 acaatgattc cttccgcact tccactgatg acattacttc ccataaagac aagattttcg
    31441 taacttgttt cgctttgctg atcgtcttca catactttcg catgcttttc tactggatag
    31501 ctttcccctg tcaatgctgc ttgggaaatg aataaatcct ttgcggacaa taaacgcata
    31561 tccgctggta tcatatcccc tgctgaaagc cgaacaagat ctccacagac gatttcttcc
    31621 ataggaatct ctgtgtactt tccattacgt tttacagctg ccgttacttt cacaagtgat
    31681 ttcagttttt cagccgcttg attggaacgt acggactgaa tcaacgtcat tgtcccgctg
    31741 acgataacca tagtgaagat aatggctaca ccgaaaagat ctttatctcc aggagctgct
    31801 aatacatatt ccgtgatgaa tgaaataatt cctaatgcga tcaacactaa tgtaaatggc
    31861 gtgatgtatg ctttcaatat ttctacaatg aatggtgttt ttttgccata tgaaatcgca
    31921 ttttcaccgt aattttctct tgaacgttcg atttgttctt ctgtcaatcc atttgctgat
    31981 gcatggaata gttcaaataa ttcttgtttc gtttttccag caaaagctgc atagattttt
    32041 tgtgtatctt tctttttttc catctcaatc tctcctttgt gtccaaaaga cagctgttct
    32101 cgctgctttt ggtctttagc tattcatcgg atcgatagag aaaaaactga aaggggctga
    32161 gaacagatag ataatagccc tggcctttcc tctagatcct tgtttttcga atgactgtca
    32221 tccatttgtt ctcatctcct ttactaagta attttaattc atgacttcca atcatttttc
    32281 gaatagttat ttttccaatt agaccacctc ctttttattt ttcaccgatg gggatttgcg
    32341 gcaacaaaaa aacctctcgc caaatactca gcgaaaggtt tgaaaaagca tattaaaaaa
    32401 agcttctcag atctcatcgt tgagttttga cactgtacaa cgtaaaaagc agatacctgc
    32461 ttttagctac cttatgaaaa gccttatctc gacaattcct gttctaccca ttggcgtctc
    32521 tcgacgtttt tgggcagtag cctatgtttg tataggagcc tcacctaaca aatcttattc
    32581 ggttttcctt cgttaatata acaactaaaa aagaggatgt caaatcaatt ttccatgttt
    32641 ttcatacttt ttttaaaata cacacgttat attcctgtct cacgcggttt tatttgtctc
    32701 attttccacc tcccgctata atagaaatat agtaggaaac gaggtgctgt tatggaaaga
    32761 acacttgtta ttatcaagcc agacggcgtg agacgccatt tagtcggaag catcatccaa
    32821 cgttttgaag caaagggact ggccattgct gaaatgaagt ttgaaactat gactcctgaa
    32881 ttagcaaaag aacattatca gcacttgact gagcgcagtt tttttgatga actgatcgat
    32941 tacatgactt ccggtcctgt cgtttatctt gtgctggtcg gcgaggaagt gatcgatatc
    33001 gtccgtaaga tggttggagc aacaaaagct gccgatgctg ttccaggtac gatccgagga
    33061 gactacgcgc tgccggggac tgaaaacatc attcacgctt ctgattcccg tgatgctgcg
    33121 gtaaaagaga tcgcccgctt tttcccgcca ttcgatataa acaaaccgtc tgaaaaagcg
    33181 gcgaagtgat acaaattcta aactgctggt ctagttgctg ggttcagcaa tggccagcag
    33241 ttttttctct aaggacctcc gttgattctt tcctaacata tattcgagca ctgtgaagaa
    33301 ttcttggtag tctttcgtct tttcttcccc taaagcagca gaaatcaatc tttgataata
    33361 atctgccatc aatgtttcat agatcactgt acttctttct ttatgtgata agctccgttt
    33421 catatcttca ttcacactac gtgccttttt caaccgacca ttttctatat aaaacgaagt
    33481 caggttcatt aaaaaagtaa agagatactg ctcatgattg ttcaactgtg ctaatcgtcg
    33541 cattcttttt ttgattttca aataattgtt ctcaatgaac cgatcactga atatccaagg
    33601 aaaatcagct agaatagata gttcaaattg accatagtct ggtatctgca tcaagtattc
    33661 ttcaatgatt cggcattcat aacgtaaaaa ttcaaattct gtctcagata cttctgtttt
    33721 ttcgaccatg tgaagattca aaaccaaata atacagatct tctgttttct taaaatgcct
    33781 acggttattg ctgatctctt tataaggatc aaagctgtct tcttgtaccc gatctagcat
    33841 tcgtcttttt ttctttaact ttattggttc atccagctgg tttcgcatcc attcatattc
    33901 tgcaagtgtc atgttcattc gctctaaaaa tgatctcaat gttacaaatg acattttttt
    33961 cactgaattt gtctcaatcc cgactagaga agtcttaaaa cagattcctt ccgctaattg
    34021 ggactgtgtc aacttcttgt tgactcgaag tttcttgatc aattcaccat catacataat
    34081 agttctcctt tttaatttaa atttttccac attcaataaa tagatacgtt aaaaaggaaa
    34141 aatacacgaa aaaaataaaa aaatgttttt ttcataaaaa ataccaacct tcaagcatta
    34201 gaatgttgtc taactactga aggttggttt gattgtttat tgattatttt tttgctgttc
    34261 aacgtctgtt cttgttactg gatataagtt ggttgttgtt ttattaccat tttttgaaaa
    34321 gacacttgtc gaatgttcgc ctttttgctt ttcgattgct tcattacgtt ccgcttcatt
    34381 tttatagtca tattttttcg gatcaatcgt ctcaaatccg tttggcacat aaaaccgcaa
    34441 gagattttcc tgattcagct tatctgaaag acttaacgca gtcttgactt tttcctgatc
    34501 tttggagatt tcctcatcga ttcctgctgc tttcgtatcg atgatttctc ctgtctgatt
    34561 attgtaagct tttcctccta ggacggtgta tttgggtgtc acaaagttcc catttcgaaa
    34621 agcaactact tgatcatgct gagaagataa taagtctgta ccaaaatgaa gataatcttt
    34681 atcatcgatt cccaatagat gtagtaatgt cggcaaaaca tcgatttctc caccgtattc
    34741 atgattgact gtccctttag tataaccagg catatggatc atcaatggca ctcgttgcat
    34801 ctgtgcgttg tcaaattcat cccatgtatc tggatcttta ccaagagcgc ttgctaaatc
    34861 tttattatct gtattcgaga tcccaaaatg atccccgtaa atcacaaaga ttgaattttg
    34921 gtaaatacca gattttttca aatacgtaaa aaattgttcc aatgattgat ccagataatg
    34981 tgccgttcga acataattat ctactgtgct attgccagta ttcaaagatg gaaagtcgaa
    35041 attcttgtca tccgtataat aaggcgtgtg gttggttacg gaaagaaatt tcgtgtaaaa
    35101 cggctgttgt agatgctcaa gatattgggc agattctctg aataaatctt tatctaatat
    35161 cccataacct aacgtttcgt cggattcatc aaaataagat cgatcaaaga aattgtcata
    35221 acctaagttt ttatatacat gatctctatt ccaaaaacta gctacattcc catggaaaac
    35281 agcgcttgta tagccttgct tttgtccaag gatcgctggt gcggcttgaa agacattgtc
    35341 tgacccaagt tctgtgaata atgagccttg aggtaaacca aatgtgccag tttccaacat
    35401 attttcagca tcacttgtct ttccctgtcc tacttcatga aagaaattgt caaagctgat
    35461 cgttgcttga ttctggaaaa tactatttaa aaacggtgtg acttcttgtc cgtctacctt
    35521 catattaatc aaaaactgct ggaaactttc taaatggaga acgatcacat ttttcccttt
    35581 agcgatccca aatgtttcgg gatttggctc tgcataatgt ttctttgtat aatctaaaac
    35641 ttcatcaatc ccattactgc ttgcatgcgc gcgaacactg ctagtcattc ctgttttgat
    35701 tccgtcataa atagtatacg catcgattcc tagatatttg acaatatatg aacggtcaaa
    35761 tgttcgctgc agcaattgtg gacggttggc ttcgctcagc gctagatttc ctgaaaaaat
    35821 aagccctgaa agacaggtaa cagcgatagc catcggtttg ccgacagggt tgcttttgat
    35881 aggtattttc ttgaccttca accaaaccag tagcccaatg aacacagcga tatccagcca
    35941 gtagatgaca tcatggggct tcatcaaaga aaaggagctt cctgacaatc cttgcgacac
    36001 tttcgaaaag cctaatacag atttgactgt aatgaaatct gtgaattccc gataaaaaat
    36061 aatattcaag tacaacaaaa gcgtatttaa aatatcaatg atcaaaagaa tagctaacgc
    36121 tggctttggc tttttaatat acaaagaaag gccaaaaaac aataaggtag tcgcaattgg
    36181 gttgatccac aagataaagt actggatcgt accttccaca cctaatgaaa aatctgcata
    36241 gtaggcaatg atcgtcttca gccagaaaaa gaaaacagct gtcaaaacga ttgctaaacg
    36301 attttggcac aattttttaa gttgtgtcac gtatatcact tctctctatt cacatttttt
    36361 cttattttgc tcctgaaaaa tgaaaatttc ctaaaaaagt tgttatctta agattttttt
    36421 attctttcta aagattattc cgtccacttt tcacagcata aaaccgctgc gatgtaagcg
    36481 attaaactaa tgaagagata aaagtcttgt ggtgaccgct tcactccacg gtattcaaaa
    36541 gccagtttct cggtattcgt acaaaaaaag aggctgtgac aaaattcttg tcgcagctcc
    36601 ttatttcttg atgcaggaca gctttttcac aacttcttcc ttaaacggtg ttctcctgcc
    36661 aactcctcgg ccacaagtca caatatttat aaaacatgag aagctgtttt ttgaaattgt
    36721 tccttgtggc tcagagttaa acggcaggct caagacctct tgatacacgg tgttcaatca
    36781 gctgaccctc ggtattagat tatgtcagga cgctaatttt acaacctctt ggtaaacggt
    36841 gttcaaaagc ttgcatctgc ggtaataagc ccaaccaaac gaaaaatata aggagctatt
    36901 ttttgaaatt gctccttgtg gctcagagtt aaacggcagg ctcaagacct cttgatacac
    36961 ggtgttcaat cagctgacct tcggtattag ctcaaaaaac ggcaaaatat gaggaactat
    37021 tttgccgttt gtttctctaa tactcaggtc aaaacgctga tttcacaacc tctgtttttc
    37081 attgtccata ggattgatat tgtccttgtg atgactcatt cgtaataatt gaggcttgat
    37141 tttcttcact tcccgttatc gtttgtccac tgccagaatc tgaaggactt gttcctgtcg
    37201 actcgttttt ttgagaagag acagttgttt tcttgacttg ttcatttgtc tttttccatt
    37261 ccgctaaacg atcggcgacc acttttttgt ctactttttc agaataagcg gtgatcactt
    37321 ttccattttt caaacgggct actttgtcgc caacatgagt caaaagcaaa acggtcaaat
    37381 gcttgtcttt aaattgatat tgtctgagag aaatcgcact aagtacgtca tcgatattaa
    37441 taaattgatt ttcttctttt ccttttggca aataaatgtc caattcgcct ttttcgtaat
    37501 tggcagttat tttatactcc gcccaccaat aagttgagaa ttcttttttg aaattatcta
    37561 ctgattcttt gcttgctacg attttttctg tttctttctg agcaagatct gctgtcattt
    37621 ttcctgaaaa tgctgcggat aaaataagca gtaagaaaaa accgaataac caaaagagaa
    37681 gaaacggtct ttttttcctc atgctttcct ccactcctat ttaattttga ctattctatt
    37741 atatacaaaa aatgaaatag aaagacaaaa aagttgaaaa agatcgtaaa taattgaact
    37801 tctttttcac aaaatggtaa gaatggagaa tttcacgcat ttgctctcaa ctattctttc
    37861 ttaagcctca aatccgtttt tatctgatac gtctttgaac caatgacccg attttttgat
    37921 tgttcttttt ccatcttgtg ctaagtctac tgaaataaag ccatatcgat tcttatatgc
    37981 attggtccac gaccagttat ccatacatgt ccacatatga tagcctaaac aattggcacc
    38041 ttcttgaatg gcttgatgga catatttcag atgagattgg acaaattcga tccgataatc
    38101 atcgtggatc atgccatctt ctttgataaa tcgtgcttcg ttttctacac ccataccgtt
    38161 ttcagaaata aagcagtcga tatttccata atgatctcgt acattgatca aaatatcata
    38221 gattccttgt tcatagatct cccatccgcg ataaggattc attttacgac caggcatttc
    38281 ataattgtcg aaataatcct caggcattgg accgttttta gtatcgattg gtgtttcttt
    38341 tgcttttacc cggcgtggct ggtaataatt gatacctaaa agatctactg tattttcttt
    38401 gataaccgtc aaatcctctg catggatcac aggcatgaga tcaagttctt ttactatctc
    38461 aatcaattca cttggaaact cgccttttac agctggatca aggaatgaac gattgaagaa
    38521 cgcatctgct atttgcgctg ctttaacatc ttcagggttt gattcgtctc ttggatagct
    38581 tggtgtcaga ttcaagatga taccaataac accaccttgc tccatttcat ggtaagattt
    38641 gatggctaac gctgaagcca acgcttcatg atagccaact tggatcgctt ttttcatatc
    38701 tgactcttct ggataatgcc atcgatataa ataacctgct tcgactggaa cgattggttc
    38761 attatgagta aaccattttt ttactttcga accaaacaat tcaaaacaac ttttggcata
    38821 tttttcatag gcttcaaccg tttctcggtt caaccatccg cctttttctt gcagatacat
    38881 cggcatatcg aaatgataca gattcacaaa gggttcgata ccgttttcaa tcaactcatc
    38941 aaaataacga tgatagaaac gaacggcttc ttcgttgatt gttccagatt catcaggtat
    39001 caaacggctc cattggatag atgttcgaaa tgaattatgc ccagtctgtt tcatcaactg
    39061 tacatcctct tgatacttcg tataaacttg cgaggttttt tcaggtccta cttgatcaaa
    39121 gaacttttgc ggttcttttt tgtaccaata atcaaaaatg ttctctcctt ttccatcgcc
    39181 tgaaatcctt ccttctgttt gtgggccact tgctgcagaa ccccaccaaa aatttgctgg
    39241 aaattgatat tttttcatca aagcacctct ttttcttttt tagttcgttt tagttgattc
    39301 catgtatgat attttccatc acaatcactt tttcattttc ttcctcttct tttttgattc
    39361 gttttaacag ctgttctgct gctcgttccc cgattttatc tgttggtggc gtgatcgtga
    39421 ttttcggaag gatactcaga tcgtttttat ctaaataatc atagtgactg aacgcaatat
    39481 ccttcccgat tctgacacct tcttgattga aacattcgat ggcaccaatc gacatattgt
    39541 aattgctgac aaaaatagca gttgcccctt tttctaataa tgcttttgcc ccgtaatagc
    39601 cgctttttct agaatagtct cctgtaaaga cctctaattg gatcgatgga tcattttgga
    39661 ccgcttcttt ggctcctgct aaacgttctc ttgctacgta gtctgtttga gtggcagcaa
    39721 taaatccgat ttttttatgt ccttgttcac catgatgagc gatcactttt gctacacttt
    39781 ctcgatcatt cactaatatt gtatcaatac ctgctggtcc ttctggacaa ttcaatgcga
    39841 taactggaat actcattttt gtgagcattt caaatccaga ccactcttct tcggataaga
    39901 agacaatcag cccatcgatc acatgcttca tcaggtattc aattttttgt tcaatttttt
    39961 ctggctgatc ggcaaacccg cttaataata agctataccc attttcttca gctactcttt
    40021 cgatccctga gacaacttcc gacccaaaac ggctggaaat cccgtttatc aaaagtccaa
    40081 tagagaagct tcgattattc ttcaaccctt tagcaatgat gttttcttta tagtccaacg
    40141 ctgaaatcgc ttctgctatt tttttcatgt tcttttcctt cagttgctga ccattcaagt
    40201 aacgggaaac tgttcccact gaaacaccag ccatcttagc cacatcacgg atcgatgcca
    40261 tacattctca ctcctgccag ttacatttga tttctctttc ttgttgttcg attatgatga
    40321 ccgcattctt tgcatagtgt tgcttgtcat caatatccat ataactaaag tcttcagtag
    40381 agcaacttcc tctcactatt aattatactt aaacaagtat ttgaaccggt tcatttttaa
    40441 tatagcattt ttttaaaaga ttgcaacact tttttaaaac gctttctgaa agtggcggat
    40501 tttttgcctt tttaaaattt tattctaaaa tttactctat ttgtgggtgt tttgtgcgtt
    40561 tttctcttcc atccgtttga agttttacat tccaatggta taatttcttt atcctatcaa
    40621 atgggggaag tattttgaag aaattaatcg tactatttat tttgtctgcg ggacttttga
    40681 cattgactgg ctgtgatttg tttggcggct ctgaccaatc ctcggacgaa acaaccgatt
    40741 cttctgtctt aaaagcggaa aaagcagata ccaccaacag tgtatatacg ctaaaagtac
    40801 gtaaagatat ggatttatcc gcgggacagt tgacatttac agataaaaaa atggaatgga
    40861 cacgcacgta tcagacacaa ggaactgatt cttctgaatc atctgaagaa aaaacagtgc
    40921 tgacagatat caagatcact accaaagatg acgaatatat tatttcagga aaagaaaata
    40981 aaaaagaaac aacagtcata tttacaaaaa tcggtaatta ccgtttgaag gatgaggatg
    41041 gcaacatcta cagtctatag tgccgtctgt tggatcttct tttgtttctc cccttctttt
    41101 ctgctatagt aaagaagcaa gatgattgta aaggagacga aatggatgat cgatagtcct
    41161 gattattgta catgtaataa caacaaaaat atccatacag aatttaatga ccaaggaagc
    41221 ttgagcatat gcgacaactg tcacaaacta gtcaaagatt catttaaaac atttgaatct
    41281 tatcctgttc aaaattatgt aaaaagtaga agaagataaa caaatccaag caaaactcac
    41341 tatggctgat ttttatgaac ttcaaactca gcttcttcct ttgatcaagc ttcatcagta
    41401 aaacaaacag tggatcattt cagtttacaa atcaaagagt aaagatttat ttatttcaaa
    41461 tatttgaata taaaataacc aaacgataga aaggcttatt ctacatcacg tagtaatttt
    41521 tctttcccgt cgtttggttt tttattttta gcctaattct tgaccttttg catgctggtt
    41581 atccgtaaaa tattttccag caggctttcc ttccaattca gctaattttt cttggatctg
    41641 acggatcacc ttttttgcat cttctggatt ttctacaaaa tcgaatttat ccccatccac
    41701 ttgtatttta ggacatacat caaaatcttc ataccattga ttatagcggg tgttcaacgt
    41761 ttcataatag gtatataacg tctcatcgct ttctagctgt tcatagtcac gcccacgttt
    41821 tttgatacgt tctagcatcg tgtccaaaga aactttgata tggaccatca aatcaggccg
    41881 tttttttgga gcaacatcat ctaactcgtt tagcatcgta tctaataagc tatcgtactg
    41941 ctgtacttcg atatccgtta cccgacctaa atcagcgttt agatggaaca acaggctatc
    42001 ttcatagatg gatcggtcta gcacattgtc atcgttcact aaagcatttt tcattgctaa
    42061 aaatcgcttg ttcaaaaaga agatttgcaa taaaaaagcg tactgctcag gatttgaata
    42121 aaagagcggc aatacttcgt tatcttcgac attttcataa aatggtttag aatcaatctc
    42181 ttttgcaagc atatccgtca aactgctctt acctgcacca atcgttcctg aaatcaaaat
    42241 cactgacata caacccttcc tcttctataa gtactcgcct acgtaagttt caacaggcaa
    42301 tgtttcaaaa tcatattcac gtacagcttg gtctgatttt aaatacgtat ggagactttg
    42361 tgtaatcatc aagttcgtag gtaccgtctc ttcttgtaca atgatcatcg gttttttcaa
    42421 catagttgcc acacctaatt cgtaagccgt tcctggatcg attgtttgat gctcaaagtc
    42481 aaggatagca acaacaaatt ctgcatttgt cagttcttcc atatcttttt cgtatacttc
    42541 ctgcgcccat ccagcgctga atagttcata attgctttct tggtgatgac gtggagagta
    42601 gaaacttgtg actgttttgt tttcctccaa cgctttttcg atacgactta ctcgatcgat
    42661 ttgctcctct gaaaaaaatg gtcctgctaa atatatttga ctcattattt tgcctccttc
    42721 aataataact gatttttaat agatactgct cgtaattcat tgaccagttc caatggattg
    42781 ttggaaaaga ttacttgatc caaccatttt ttatccataa aatcctcttt tgcaaaagtt
    42841 tctaataatt gttgcaagcc gctgtaataa ccgccaatat tcaatattcc gattggcttg
    42901 tcgggcgtca gaccgattgc tatccaagaa agaagttgtg agaattcctc caatgtacct
    42961 agacctccag ggaaaacgag gacagcatct cccttttcaa ataataatac ctttctttca
    43021 tcgatcgttt ctgtaggaat aaatgtagta acatcttttc caggcaattc agtttcaaaa
    43081 agcccattcg gatagatacc tgtgactttt ccgttatttt ccagtactgt tttactaatc
    43141 actcccataa tcccggagta tgaaccaccg taaaccagtt cgatttcttc ttgtgccatg
    43201 tattttccta acgtttgcgc aataaatttg taactttctt tattaccaaa acgggaaccg
    43261 caaaaaacag taagtttcat aaaaaaacac tccctaactc aaaagtctat cttcattcta
    43321 cacgtgtttt tttaaaataa aagactagat tttgtagttt tttagtggta tataggatag
    43381 acgccactat atatagtatt tagaaaaaaa tcatttttca gccattttat agataaaaca
    43441 aaaataaata acgtgttttc atcgatatga aatgacttac ttatagaaga tactgggatt
    43501 tcgtcctata tgccaataac gtgtattatt ttaaacaaca aatacttgtt tcttttatta
    43561 acgaaaaaat cattttgttc tcgtgttatg tttcactgat aaatgatgtt tctctaaata
    43621 aaaaagccga cgaactaaac gatcaaaatt tgatcatttt tgttcatcag cttctcctaa
    43681 aaatcttttt gttaacgata aatagctgga tttgtaaaaa tatcacttga atcgtcactg
    43741 ctggaagaaa attcagtgat aaccgcacct gttgcgcctg cctggaacta gtgtttgata
    43801 ttgggctcga acgtgtactg atctctagca tgaagaataa tttctttttt tactgtataa
    43861 taactttctt tcctttttgg aatagtacag gaaagttttt ttgtttcctt tccttcgaca
    43921 taaggtatac cagacccttt cgacaacgaa aggttctcat ttttccttca tcgtcatttc
    43981 aaggagggtg ccgatgctct ggacagtttc gatcgctcct aaaccaaaat ctgtatattc
    44041 gattctttct aactctgatt ctttcatacc attcctgtct caaggtacat ctattgtact
    44101 tttttcttgt aatccattct gcctctccta actaattgtc aaatcatccc aagatatgtc
    44161 ttcttcctct ttttcttctt ggatgatttc tgcattcgga tctactggtt ttttcaataa
    44221 taaaatggtt attcctgtga caaaagaacc agctaacaat gcaccaatga atgcccatgg
    44281 tctggtcatc gtagcgactg caaacattcc cgatgccgga atacggctat ctgcgcccca
    44341 aaacatgctt aatccgccac ctactgtccc accgactccc gttgctatga cattgcgtgc
    44401 caagtcgttc aatacgatag ggaaacatcc ctctgtgatc tgacagatcc ccataggaaa
    44461 agcggtcttc aatgtatcga tttcttgacg attgagtata tttttacgaa tgattttccc
    44521 taagaaataa gcgatcgtca agccgaatgg ggttaccatc gaagctaaaa tcaatacagt
    44581 gattggttca tagattcctt ctgaaaatcg tgccacaccc ccttatccag aataaatggt
    44641 aatccagaaa cagccgtgta tctgcggtaa taagcctaac caaacgaaaa atatgagaag
    44701 ctatttttgt ttggctgttc ttattacttg atgcagggca gctttttcac aacctctttt
    44761 tttatttatg tttcataatc aatatggtaa ggatgcagct ccgtatggta gaccgcctcc
    44821 gaatattcaa tcatttcccc aatttgcgta gacgatttcc gtatccgttt gatgccgatc
    44881 tgtgcgtctg ttttcagaat tccttgttct tcttctgtta aaaatactgc cgagaaatca
    44941 tcagaaaatg agtcaatagt atatcctttt ttcgtcagca aacgatacaa agattctttg
    45001 ccaaattctt ctctgctgaa ttgcatcatt tcttgtggaa gataatgaac aaaataaata
    45061 taaggctgat tgttcaaata atataagcgc ttaaaacaaa cagcttgctc accgaaaaag
    45121 gaaaacaaag gatcttcctt ctttatatgg accaattgca aatccagcgt ttctttccgg
    45181 acttcatatc gggagttctc caagatttgc gtaaagcttg ccgctttgga taatcgattg
    45241 taaggacgat tgcttagaac cgtcgtccca cgaccacttt tctttgcgac atattcatct
    45301 gatgccagca gttcgattgc ctttcggaca gtgattttgc tgacttgaaa cagttcttct
    45361 aattcacttt ctgtcggcaa catcgaaccg actggatatt ttcctgagaa aatatcttct
    45421 ttaattttat ctgcgacttc caagtatagt acacgtgtcc ttttcataac gtcacccttt
    45481 gctttactat tactaaaaga atagcacata aaaaataaaa aaagaacaca aatcacatgt
    45541 gagaagtgtg ttctttttca ttcagctgct ttaattacaa gtttacgcca tttgaggcaa
    45601 ttacttcttt ataccaatca aaagattttt tctttgatcg attcagtgtc ccatttcctt
    45661 catcgtctag atccacatag ataaagccgt aacgtttact catttctcct gttgatgcac
    45721 tgacaagatc gatacatccc caaggcgtat agcccatcaa atctacaccg tctgctactg
    45781 cattagccat cgcttcgata tgacgacgta aataatcgat ccgataatca tcaataatcg
    45841 tgccatcctc ttccatatga tcaatggctc caagaccgtt ttcaacaatg aataatggtt
    45901 tctggtaacg attgtataat tcattcaatg cgattctcag cccttctgga tcgatctgcc
    45961 agccccattc acttgcctca agaaacggat tttttactcc tcccagcaag ttcccttctg
    46021 aagttgctgc ttgtggatca gtctcattga ttgccgtaga catataataa ctaaatccga
    46081 tgtagtccac tggatattct tttagcaact ggaagtcttt ttccgtttgc tctaattttt
    46141 ccggatcgac tccatatttt ttatgggtac gcgctgtgta tgcagggtat tctccacgta
    46201 cttggacatc cgtgcagaaa aaattgaatt cttggttttg gatcattgtt gctgcctgat
    46261 tgactggatt ggcatcgatg ctgtaagttg ttgcatagat aatcatgcag ccgatttgga
    46321 tatccgaacg caattcgtgg ccgattttta cggctttgct acttgcaacg aattgattat
    46381 gccatgcttg gaaaatattt tggtactctc ctgctccatt gctttttact aatccttgac
    46441 ttaaagctgg aaagtgaaag gccgaattga tttcattgaa agtcatccaa tatttgactt
    46501 tactgctgta tctctctaat actgtctgag caaatcgttc atagaagtcg atcagttttc
    46561 gatttttcca gccaccgtat tcttttgcca aatgaagagg catttcgtaa tgagaaatcg
    46621 tgatcacagg ttcaatatca tattttaagc actcatcaat cagctgatca taaaattcta
    46681 atcccgcttc gttaggagta ccttcgtctc catttggaaa aattcgtgtc caggcaatcg
    46741 aaaaacgata acatttgaat cccatttccg caaacaaggc aagatcttct ttgaaacgat
    46801 cgtaatgatc gattcctcga tgattcgggt aaatatattt ttcttgatca atcgtccagt
    46861 caaattcttc actgccgata atcgcaaagc gctgcttacc tccaggcata gcatctgcaa
    46921 cggatagtcc ttttccgtct cgatcataag ccccttctaa ttgatttggc agcagttgct
    46981 ccaccccata aaaatttttc tgaaaaacgt gattgcatcc tgatttcctc cttattttac
    47041 aattgccaga acatcttcgt ttgcaataat ctctttttgg tctaattcaa gtatatcggc
    47101 aaatgcatca gtattcgtaa tgacaactgg agtgattacg ctatagccag ctgctctaat
    47161 tgcttcgatg tcaaaacgaa tcaataaatc tcctgctttg acgagatccc cttgtttgac
    47221 taagatctca aagccattcc catttaattc tactgtatcc atcccgatat gaatcaaaat
    47281 ctccgctccg tcagtcgttg tgatccctac agcatgtcca gttggaaaaa tagtcgtgat
    47341 ctctccgtct gcaggtgcaa ctagttctcc tacttctgga tcaatagcga taccttttcc
    47401 catcacgcca gaagaaaaaa cttggtctgg tacttcactc aatggcagta ctttacccgt
    47461 taaaggactt tttaaggtat gccgtgctga agtaatcggt tctcctgcat tcgcatgttt
    47521 gttttctaat tgattgtctt gtgtttgttc gtcaaatcct aaaatcaatg tacctacgaa
    47581 tgcaagaaca aaagcaattc ctgtagctat tactgctact gtcacatttg actctacacc
    47641 atccaccgta ttaatgaaag tcggaatcgt caataagctg accaaactgc tggagaaagc
    47701 ttttactccg gaaaatccga taatagctcc accaatacct ccagaaatac aagcggcaat
    47761 gaatggtttc ttcaaaggta atgtcacacc atacacagtt ggttcagtga ttccaaataa
    47821 cgaagtcact gtagaagaaa tccctaatgc tcgaagtttc gtatcttttg ttcgcaatgc
    47881 tacagctaaa gcagccccac cttgtgccaa gatggctgga agaagcattg gcatcaatac
    47941 atcaaaccca tattgttcga tattcaaaaa catgatcggt acgaatcccc aatgcatgcc
    48001 gaacatcacg aatacttgcc ataaacttcc catgattaga cctgctacga ttggactaaa
    48061 tccataaatc gagttgaaga gaccgcctaa agcattaccg gcaaccgtcc cgattggtcc
    48121 taaagcaaga aatgttaatg gaaccatcac tactaaaacg acaagcggaa ctaaaatcat
    48181 ttgcagaaat tgcggaatca ctttttttac gaatggctct aatttacttt gaacccatac
    48241 ggctagaatg attggtatga cagaagaagt ataaccagat ggtgaaagaa tcactgggat
    48301 cccaaagaaa ctgatagctt ctcccgcacc agcaactgct gtgatagctg gatacacaag
    48361 tgccatagca attgctactg ctaaaaactg gttggctttg aactttttag ctgctgtaaa
    48421 agcaagcatg acaggtaaga aggtaaagac accatctgct gcagcaaaca atatttgata
    48481 agtaccgcta tcagctgtca gccagcccat caccgttgcc aatgataaga atcctttcaa
    48541 taccccggcg gctgccatcg cccctagaaa aggtgtaaat atactagaga taatatcgat
    48601 cagacgattc aaaatatttt tggactcttc ttttccatcc tccgaaactg caagaccttc
    48661 ctcatccacg atcgattgat aaacatctgc tacattactg ccaatcacta cttgatactg
    48721 accgccactt tgtacgactt ggatcacgtc cgggtcttct tttaactttt gcgtatctgc
    48781 taaagattca tcttttaatc tgaatcgcaa ccgcgtagca caatgtacta aacttttgac
    48841 attcttttgt cctcctaccg cttcccatac acgacgacca actgcttgat tatccattgt
    48901 tttttccttc tttctttttt atttaaaaaa taaaaaccca gattgaaaaa aacagtcaca
    48961 aacattcgtg aacctatttt ttcaatccag gttttgcctg ccatacagta acaatcctta
    49021 tgattggatg ctttttttct cacttactac tctttgaata tgaatcgtta aataaatctg
    49081 ctctgcgatc cccatttcct gtttttgcgt ttgtcgaaga tactcattga tccgttcgac
    49141 acaatgaaac gcttttggat actttccttg aactaatgcg tagagaaatt catcttgctc
    49201 gtcagatcct tcccctttga gataacgctg ggcaaaaaac tgcagatgag taatgatacg
    49261 ctgataattc aaagattctt catcgaacac gatgccgaag aatttactga tgatactcag
    49321 tatatctctt accattgtgg tagcggtcat cgtcacttgc atgtcttcgt ttccttgttg
    49381 gctattcaca aaatgaaagg caataaaacc ggcttcatcc tcagaaagtt ccacacccgt
    49441 cttttgtttc acgaacaaaa gagcctcttt cgctgcttga tattctttcg gataaaattt
    49501 ttttgtttca aataataatg gattaggcag ttggatattt tcttttgctc gtgtgatcgc
    49561 ataatgaaga tgatcggtca atgtcagata aatattaggc gaaaattcaa cttttagttt
    49621 atcttcagcc agttcgacta cttgtttgac ccattcgatc tcctctgccg gaatatcagc
    49681 catcagctgc tggaattggt ttttagcaaa agaatctctt aaaacaaatt ctttttggac
    49741 cgcttctggc gaaatctcgt ccccaatctt tttttgaaaa gctagtcctt ttcccataca
    49801 aacgacttct tccccgaatc catttttcga aatgacgaca ttgttattca aaattttatc
    49861 aattttcatt ttgcttcgcc ctctcaagaa aaataaaaaa acctagaatt cgcctactgt
    49921 ttcttagctg aattctggtt ttgcctgccg tacagtaaca atccatttct tattcaaaat
    49981 catcatacca tcgaattttt atttatgcaa gccttttcat ataaaaaata aaaaaactgg
    50041 aacacaagtc tatctagaaa a
//
LOCUS       DAZUKC010000010        49446 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000010 DAZUKC010000000
VERSION     DAZUKC010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.010"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(240..1967)
                     /locus_tag="ACG80T_000537"
     CDS             complement(240..1967)
                     /locus_tag="ACG80T_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFS4840216.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(1960..3153)
                     /locus_tag="ACG80T_000538"
     CDS             complement(1960..3153)
                     /locus_tag="ACG80T_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS4840217.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3340..3984)
                     /locus_tag="ACG80T_000539"
     CDS             complement(3340..3984)
                     /locus_tag="ACG80T_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4840218.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQAKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4078..4212)
                     /locus_tag="ACG80T_000540"
     CDS             complement(4078..4212)
                     /locus_tag="ACG80T_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFS4840219.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4214..6346)
                     /gene="feoB"
                     /locus_tag="ACG80T_000541"
     CDS             complement(4214..6346)
                     /gene="feoB"
                     /locus_tag="ACG80T_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFS4840220.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6343..6816)
                     /locus_tag="ACG80T_000542"
     CDS             complement(6343..6816)
                     /locus_tag="ACG80T_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFS4840221.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7217..7441
                     /gene="nrdH"
                     /locus_tag="ACG80T_000543"
     CDS             7217..7441
                     /gene="nrdH"
                     /locus_tag="ACG80T_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFS4840222.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7600..9759
                     /gene="nrdE"
                     /locus_tag="ACG80T_000544"
     CDS             7600..9759
                     /gene="nrdE"
                     /locus_tag="ACG80T_000544"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFS4840223.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            9809..10774
                     /gene="nrdF"
                     /locus_tag="ACG80T_000545"
     CDS             9809..10774
                     /gene="nrdF"
                     /locus_tag="ACG80T_000545"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFS4840224.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(10863..12002)
                     /locus_tag="ACG80T_000546"
     CDS             complement(10863..12002)
                     /locus_tag="ACG80T_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS4840225.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12311..13024)
                     /locus_tag="ACG80T_000547"
     CDS             complement(12311..13024)
                     /locus_tag="ACG80T_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFS4840226.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13278..13979)
                     /gene="nagB"
                     /locus_tag="ACG80T_000548"
     CDS             complement(13278..13979)
                     /gene="nagB"
                     /locus_tag="ACG80T_000548"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFS4840227.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14213..15745
                     /locus_tag="ACG80T_000549"
     CDS             14213..15745
                     /locus_tag="ACG80T_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS4840228.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(15936..17138)
                     /locus_tag="ACG80T_000550"
     CDS             complement(15936..17138)
                     /locus_tag="ACG80T_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4840229.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17220..17909)
                     /locus_tag="ACG80T_000551"
     CDS             complement(17220..17909)
                     /locus_tag="ACG80T_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFS4840230.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18015..18383)
                     /gene="rplL"
                     /locus_tag="ACG80T_000552"
     CDS             complement(18015..18383)
                     /gene="rplL"
                     /locus_tag="ACG80T_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFS4840231.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(18436..18939)
                     /gene="rplJ"
                     /locus_tag="ACG80T_000553"
     CDS             complement(18436..18939)
                     /gene="rplJ"
                     /locus_tag="ACG80T_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFS4840232.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19143..19270)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(19349..20038)
                     /gene="rplA"
                     /locus_tag="ACG80T_000554"
     CDS             complement(19349..20038)
                     /gene="rplA"
                     /locus_tag="ACG80T_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFS4840233.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20142..20564)
                     /gene="rplK"
                     /locus_tag="ACG80T_000555"
     CDS             complement(20142..20564)
                     /gene="rplK"
                     /locus_tag="ACG80T_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFS4840234.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            20907..21575
                     /gene="sdaAB"
                     /locus_tag="ACG80T_000556"
     CDS             20907..21575
                     /gene="sdaAB"
                     /locus_tag="ACG80T_000556"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFS4840235.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            21588..22460
                     /gene="sdaAA"
                     /locus_tag="ACG80T_000557"
     CDS             21588..22460
                     /gene="sdaAA"
                     /locus_tag="ACG80T_000557"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFS4840236.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(22551..23651)
                     /locus_tag="ACG80T_000558"
                     /pseudo
     CDS             complement(22551..23651)
                     /locus_tag="ACG80T_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(23743..24630)
                     /locus_tag="ACG80T_000559"
     CDS             complement(23743..24630)
                     /locus_tag="ACG80T_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS4840237.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(24707..25252)
                     /gene="nusG"
                     /locus_tag="ACG80T_000560"
     CDS             complement(24707..25252)
                     /gene="nusG"
                     /locus_tag="ACG80T_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFS4840238.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25353..25523)
                     /gene="secE"
                     /locus_tag="ACG80T_000561"
     CDS             complement(25353..25523)
                     /gene="secE"
                     /locus_tag="ACG80T_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFS4840239.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(25547..25699)
                     /gene="rpmG"
                     /locus_tag="ACG80T_000562"
     CDS             complement(25547..25699)
                     /gene="rpmG"
                     /locus_tag="ACG80T_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS4840240.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            25861..26505
                     /gene="cbpA"
                     /locus_tag="ACG80T_000563"
     CDS             25861..26505
                     /gene="cbpA"
                     /locus_tag="ACG80T_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFS4840241.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(26561..27064)
                     /locus_tag="ACG80T_000564"
     CDS             complement(26561..27064)
                     /locus_tag="ACG80T_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFS4840242.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27077..27123)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            27661..28626
                     /locus_tag="ACG80T_000565"
     CDS             27661..28626
                     /locus_tag="ACG80T_000565"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS4840243.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(28725..29897)
                     /locus_tag="ACG80T_000566"
     CDS             complement(28725..29897)
                     /locus_tag="ACG80T_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4840244.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(29884..31029)
                     /locus_tag="ACG80T_000567"
     CDS             complement(29884..31029)
                     /locus_tag="ACG80T_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4840245.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(31043..31967)
                     /locus_tag="ACG80T_000568"
                     /pseudo
     CDS             complement(31043..31967)
                     /locus_tag="ACG80T_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            32084..33163
                     /locus_tag="ACG80T_000569"
     CDS             32084..33163
                     /locus_tag="ACG80T_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFS4840246.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            33160..33788
                     /locus_tag="ACG80T_000570"
                     /pseudo
     CDS             33160..33788
                     /locus_tag="ACG80T_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(33898..34125)
                     /locus_tag="ACG80T_000571"
     CDS             complement(33898..34125)
                     /locus_tag="ACG80T_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4840247.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(34228..36816)
                     /locus_tag="ACG80T_000572"
     CDS             complement(34228..36816)
                     /locus_tag="ACG80T_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFS4840248.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(36836..37495)
                     /locus_tag="ACG80T_000573"
     CDS             complement(36836..37495)
                     /locus_tag="ACG80T_000573"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS4840249.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(37544..38047)
                     /gene="trmL"
                     /locus_tag="ACG80T_000574"
     CDS             complement(37544..38047)
                     /gene="trmL"
                     /locus_tag="ACG80T_000574"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFS4840250.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(38268..39134)
                     /locus_tag="ACG80T_000575"
     CDS             complement(38268..39134)
                     /locus_tag="ACG80T_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFS4840251.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            39243..39884
                     /locus_tag="ACG80T_000576"
     CDS             39243..39884
                     /locus_tag="ACG80T_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFS4840252.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(39946..41307)
                     /gene="mgtE"
                     /locus_tag="ACG80T_000577"
     CDS             complement(39946..41307)
                     /gene="mgtE"
                     /locus_tag="ACG80T_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFS4840253.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(41325..42224)
                     /locus_tag="ACG80T_000578"
     CDS             complement(41325..42224)
                     /locus_tag="ACG80T_000578"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFS4840254.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(42226..43023)
                     /locus_tag="ACG80T_000579"
     CDS             complement(42226..43023)
                     /locus_tag="ACG80T_000579"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFS4840255.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(43001..43705)
                     /locus_tag="ACG80T_000580"
     CDS             complement(43001..43705)
                     /locus_tag="ACG80T_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFS4840256.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            43836..44411
                     /locus_tag="ACG80T_000581"
     CDS             43836..44411
                     /locus_tag="ACG80T_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFS4840257.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            44529..45206
                     /locus_tag="ACG80T_000582"
     CDS             44529..45206
                     /locus_tag="ACG80T_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFS4840258.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(45285..47093)
                     /gene="pepF"
                     /locus_tag="ACG80T_000583"
     CDS             complement(45285..47093)
                     /gene="pepF"
                     /locus_tag="ACG80T_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFS4840259.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSDAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(47269..48267)
                     /locus_tag="ACG80T_000584"
     CDS             complement(47269..48267)
                     /locus_tag="ACG80T_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFS4840260.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(48493..49146)
                     /locus_tag="ACG80T_000585"
     CDS             complement(48493..49146)
                     /locus_tag="ACG80T_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFS4840261.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        14780 a        10393 c         8720 g        15553 t
ORIGIN      
        1 tttgagaatg cacttcgtac ataataaact ttaatcacat tagtctttca tacctacgat
       61 cgatctcttt gactcgcacc cataaattca taatagtttg tcggcgtctt tggacgtttc
      121 gaagatgagc tacgcattct ctttttttat agacaaaaaa gatagagacg aacatggagc
      181 ttccaaattt ggatatttga tccaattttg gaagctccat ttatttcgtg tctgtctttt
      241 tattttttcc agctttcgac taattcttgg ttctctttga tccagtcttt tgctgcttgc
      301 tgtggatctt ttccattgtt gatttccaac ataacttttt ccatatcttt ttctgaccaa
      361 tggaaattgt ctaatacttt gtaagcttct ggctggtctt ctttcaatcc ttttcgaacc
      421 atcgtatgga tctgctcttc gcttcccatt ccgttttcag gatcttctaa gtatttcaga
      481 tcatatttgg caaacatcca atgaggtgtc catcctgtaa ctacaattgg ttcgtgtttt
      541 ttgatcgctt gacctagtgc aacggtcatt gctcctgaag aagatgtctc tactttccag
      601 tctttcaagt tgtcatattt ttggatcgtg ttttcagcag ccgtaactac accagctcct
      661 ggctcgattc ctatgatttt cttacctgcc tgatcggtta actcatcgat cgaatttaca
      721 tccatgtagg ctggcactgc taaaccaact tttgctcctg tcaaattggc acctaggtct
      781 tcgacttgat ctttatattg tgcatattgt gaaccatgag ttttaggtag ccaagctgaa
      841 accatggcat ctgactcgcc atttgaaaca gatttccaca tgattgaatt atctaatggc
      901 gtcatcgtta catcatagcc catttgtttc aatacttcac cgactacatt tgttgaagca
      961 acttctgtat cccattcaac ataagataag ttgatacgtt ttgtttcttt ggcagatgaa
     1021 aaactcccta taccaatcgc acctaagatc actactacta cagcaccaat gatcagtcct
     1081 tgacgttttt tacttttctg tgttgttgct cctgcagctt ttttattgtt tggctggttc
     1141 aaatgctgtg taaaacggtc aatgatgatt gctaagataa ctaatgcaac cccgttgaca
     1201 aatccattcc cgacttgtgc tctttgcaat gctgataata cgccgcgtcc taagccgggt
     1261 gcaccgatca tagaggcaat aacgaccatt gaaagtgaca gcattgttgt ttgattgatc
     1321 cctgccatga tcgtactttt agctaaaggc aattctagtt tgaatagttt ttgacgactt
     1381 gtgctcccga atgaatcaga agcttctact aattctttag gaacttgtcg aatccctaaa
     1441 ttcgtaaaac gaacggttgg tggcagagca aagatgactg aggcaaatac ccctggaacc
     1501 atcccgatac cgaaaaatgc aactgctgga atcaaataaa cgaatcctgg cattgtctgc
     1561 ataaagtcaa ggatcggttt gataatactt tgggctttac tgctttttgc catcaagatc
     1621 cctaatggta ccccaatgat gatcgagatc acactagata aaagaacaag tgtgacagta
     1681 ttcatcaaat cattccatag accttggtta taaataaacc atagaccgat taatgtgaac
     1741 aacgtaagtc ctggacgttt cttagaaata aagaaagctg ctaccgtcaa taacaagata
     1801 aacaaaggag ctggaatcac taacagtaaa tttgtaatcc ccgacatcac tgcctgtcca
     1861 atcgtttgta aaaatgaaaa taggccagaa aaagttgtcg taaaccattc tgtgatattt
     1921 tctacccagt ttgccactgg taattgatag ttcaatagat tattcatgtt cactcacctc
     1981 gcttgtttct gctaatgctt caatcacact acctcggatg acgacacctt ttaattttcc
     2041 atcctctaca actgcaagtg gagtcggtga gtcataaata atattgaaaa tatcacttac
     2101 caccatgtct ttcccaatca ccgtcacatt ttcatctagg aagtcgatca atggacgttt
     2161 ttgcttccta gcttctaacg cttgatctgc agtgatgatc cctttcaggt gtctctttct
     2221 gtcaacagcc atcagcatgc tgacttcttc attacgcatc cttgtcaatg caacgtttgg
     2281 tccatcagat tcgatattcg tcgtcaatgc aggtaccatg atgttttgag ccgtaagcac
     2341 tttggaacga tcgacttctt ccacaaattc acgtacataa tcatttgctg gattggtcaa
     2401 tatttcttca cctgttccga tctgcatgat ttcgccgtct ttcatcaagg cgattctgtc
     2461 cccaatccgt aaagcttcat tcaaatcatg ggtaatgaaa ataatcgttt tttgaacatt
     2521 ggcttgcagg tccagtaatt cgtcttgcat ttctcgccgg atcagcggat caagagccga
     2581 gaacgcttcg tccatcagca agatctcagg atcatttgct aaagctctgg ctaggccaac
     2641 ccgttgttgc atcccaccag ataactgact aggatattgg tctttaaatg aaagcaaact
     2701 agaattttct aatgcttttt ctgctttttc ttgtcgttct tctttaggta caccacggac
     2761 ttctaacccg tattctgtat tttccaagat cgttcgatgt ggaaacagac cgaagttttg
     2821 gaaaaccata ttgattttat gacgacggac ttctcgcaat tcttctttgc ttaacttcga
     2881 tacatcttct ccatcaatat agatactgcc cgaagttggc tcaatcagac gattcaatag
     2941 acggatcaat gtggatttcc cactcccgga aagccccatg atcacgaaga tttctccttc
     3001 ttttacgtca aaattcacgt catacacacc aaccgttgca ccggttttct ttaaaatctc
     3061 cgttttcggc tggtgatcat tcatcatatc taaagcggct tgtgtctttt taccaaatat
     3121 ctttgttaaa tgttttactt ctacttttac caaacaaatt ctcctttttt atgtaaattc
     3181 atttttcttt ctgaaaaaag tgaccacact atgttaacat catgtagtca ctttgtcaaa
     3241 aatgaaagaa atttgcttgt ccaaactgaa aaaaacttgg ctaaatcttt gtaaataaaa
     3301 ggtttagcca agttaatatt tttttaatat ttcacacaat cattctgcaa gctgatgctc
     3361 cgggtaaaaa aagtttgtca tacgctgtag tgtatattca tttccaataa aatgaacgat
     3421 gtcccctgcc gtaaatttag cataggggcc aggtgaaaca agcagttctt cattatgaga
     3481 aattgcgata actgtggcgc cagtcgcctg ccagatattc aactcgctaa ttgaacggtc
     3541 cagatgcatt gctttttctg ataaagataa ttcaaacgga agtaaaggat tcatcttatg
     3601 tattcgcttc gcttgattga ccagctgatc caataattga gaaaaatgat tcagctcatc
     3661 ttgttgcttc tccacacttg ccatgatttc tgcttttgta tcttggatgg attgtacatc
     3721 ttgatattgc aataggaaat cttttgcttt ttccttcgaa gcaacaaatg ccccacttcc
     3781 atgccgtact tccatgattc caagatctac cagcacattg atagccttgc gggtagtctc
     3841 aggagaagca ttgtacgtac tggcaagtgt tgagcgggca tggatcttct cacccacttt
     3901 atatcggttt tcaacgatac gttcggctaa atcaacagca atctgctggt agcgcgggcg
     3961 gacgattttt ttctttccag acattttctt tcctctttct ttttttactt tccctcatct
     4021 tactatgctc tagcaaaata agcaaaaaag gggagaatag cctcttgcaa cgactgatca
     4081 tcgcttgatc tgttcctttt ttactgggca agctgtttga cagtcatcac aattcttacc
     4141 ggattttaaa cgagtataga caattgcgcc tgcacttcca aagattaaca cacttaataa
     4201 gaatgtcgcc atcctattcc tctccttcta atgacaatat agtataaaac tggttcttgg
     4261 attttggctg acggaacaaa acataaacga gtcctatcat cagtaaaata gcaagatacg
     4321 tccctaacgc tacctgtcca ccctccaata agtgtcccaa ctgataaatc acaaaactaa
     4381 ctaaataagc taaaccgcat tgatagccta tggcgatcca agtccatttc ttggcattca
     4441 tttcccgatg gatagcccca attgccgcaa aacaaggcgc acaaagtaaa ttgaaggcta
     4501 acaatgaata agctgctgcg ggagtatatg tgtgttgtag cacttgccag atttctctgc
     4561 cattttcgga tacttccgca tgaccaaaca aaataccaaa tgtgccaata atattttctt
     4621 tagcgaccaa tcctgtaatc gctgcgactg ttccttgcca gtttccccaa ccaagcggct
     4681 gaaaaacaac tgccaggatt cgcccgaaaa aggctaagat actttggtct tcagaaacag
     4741 gttgagcata aaaattataa gaagagctga accagatcac gatactagaa acaaagataa
     4801 tcgttcctgc ttttttaaca aaagacttgc tgcggtcata ggtttgtctt aggaccgctg
     4861 accattgagg aaaatgatat gcgggcaatt ccatgataaa aggagctgta tctccagcaa
     4921 acaaacgtgt ttttttcaag ctgattccag agaaaataat tgcagtcata ccaagaaaat
     4981 aggcagaagg agctacccaa gattgatgag gaaaaaaggc ccctgctact aaggcaataa
     5041 tcggcaactt tgcagaacaa ggcatgaagg ttgtgaccat gatcgtcaaa cgacgatctt
     5101 gctcgttttc aatcgtgcga ctggccatga cgcctggcac tccacatcct gtagcaatca
     5161 gcataggaat gaaggatttt cctgaaagac cgaatttcct gaaaagtcga tccatgacaa
     5221 aagcgatcct cgccatatat ccgcagtctt ccagaaagct caagcataaa aacaatataa
     5281 tcaattgcgg tagaaatcct aacactgctc ccacgcctgc gatgatgccg ttcaagatca
     5341 gctcctgcat ccatcccgct acttgccatt cagctaacaa tctgccaaca tgttctggta
     5401 accatgtacc aaacaatgtg tcattgatcc aatctgtccc cattgttcct actgtttgaa
     5461 tagccagata atagatcagc cacatcacaa atgcgaagat cggcaaggct aaccaacgat
     5521 tcgttgcaat ctgatcgatt ttgtcactca tcgataattg gaaagaatca tttgacacaa
     5581 cacagagtgt acatagttgg gtaataaatt cataacgttc attgaccaga atcgtctcac
     5641 tatcgtcgtc caatagtttc tctgttaact ggatcagttg ctcgatctct ttttgctgag
     5701 aaactgctat ctcgtaatcc tctcttacct tctgatctcg ttcaaaatat ttcattgcat
     5761 accagcgttt cttgccgctt tgttctccta gtacatcttc aatttcatgt aaagctgctt
     5821 ctaaacgtgg atcatatgta cagggaacag gaatagaagg ccgctttttt gcagactcct
     5881 gaatcttttc gattagtttt tcgattcctt gctttttcaa tacgctgatt ggtaaaactg
     5941 gtacaccaag accataggaa agcttttcta gattgatttt ttttccttct tttttgatga
     6001 catccatcat attcagacca atgatcatag gaatgtctaa atccaataac tgcgtagtta
     6061 aataaagatt tcgttctaaa ttggtagcat caataatgtt gatgatcagt tcaggctgtt
     6121 cctgaaccaa ataatctctt gcaatcactt cttctggtgt ataaggagac aaagaataga
     6181 tgccgggtag gtcctgaata tgaatgttcg gatttttttt caacgatcct tctttttttt
     6241 ctactgttac gcccggccaa ttccctacat attgattcga accagtcaag agattgaaca
     6301 agctagtttt cccgctgttt ggatttccga ctaacgcgat attcattctt tttcctccct
     6361 ttgtatcaag attagttcgg cctctgactt tcgtaacgtc agttcatacc ctcgcactgt
     6421 cagttcgatt ggatctccca gaggggctag ttttcggata actattttta catttcgcgt
     6481 caatcccata tccattaacc gtcgcttcac tgcgccttgt ccgtggattg ctacgacgat
     6541 cccagtctct cctgctttta attgatcaag agaagtccag ctttcagagg aagcagtctt
     6601 tttattgaca aagatttgac ctagtatttc ccgacttact gccactcgat gactttttac
     6661 cagtaaaatg gcattgttgc ctgataaatt gacgacgacg attttacttc cggcaacgat
     6721 cccgatattt tgcaaatggc gttttgtttt ttcttctgtt tcgattcgtt cgacttgata
     6781 aacgaaattc tgctttgcct gcgctaaggt ctgcatattg attcctctct tgtgaaataa
     6841 atcatgtatt aaaatcattc attatatgtt ctaattttct tacgtgttac gcaaataaat
     6901 gagaatcatt ctcacataga atactctcgg taattgagaa tgtcaattat acttgtgata
     6961 ttatgagcga atctttagta ttgatagata aaaatgagaa aaaaatattt cttcatagtt
     7021 ttccacaatt ttttctctat taaatttgct attttcccac acgatacttg ttttcagctc
     7081 ttttttcaaa aacacaaaac ttagtattcc acagtttcca caacacaata tgtagtgttt
     7141 gttgtttgac ttttttacta agagttttat actatctatt atagataata agaatgtgtt
     7201 agaggaggca ctcaccatga acgtaaaaat cttttctaaa aataattgta tgcaatgtaa
     7261 gatggtcaaa cggtttttaa cagaaaatca aattgctttt gaagaagtca atatcgatga
     7321 acagcctgaa gcagtagact ggttgaaagc acaaggattc caaagtgttc ctgtcattac
     7381 ttctgatgca gaaacagtta tcggattccg tcctgatcag ttgcgtaaac ttgccagcta
     7441 acatgactaa aagaatcaaa acgtgataca tggatttgaa gggcttgaga cagcaccagt
     7501 ttttgtcgca aacttccaaa tcctttttta aagcttaaaa acaatgcaat gatttgcaat
     7561 caagcatcat caaatacgaa atgaaggttg ggcgtacgta tgagtcttaa aaatttacaa
     7621 gatgtcagtt atttcaagct gaataatgaa atcaatcggc cagtcaatgg tcaaatccct
     7681 ttgaataagg ataaagaagc tttagttgcc ttttttgagg aaaatgtcaa accaaatacg
     7741 atgacttttc tatctattat ggaaaaaatc aattacttga tcgatcaaga atatatagaa
     7801 gcagaattca ttcaattgta ttcaccatca tttattgaaa agctatatca atttttggac
     7861 gagcaacatt tttctttcaa atcttttatg gctgcttaca aattttattc tcaatatgcg
     7921 ttgaaaacca acgacggttc cgcttatctg gaaacttatg aagatcgagt agccttcaat
     7981 gcgctttact ttgcgaacgg ggatgaagaa ttagcacttg ctcttgcaga cgaaatgatc
     8041 catcaacgat atcagccagc tacaccttct ttcttgaatg ctggacgtaa acgtcggggc
     8101 gaattggtgt cttgtttctt gactcaagtc actgacgata tgaactctat cgggcgctca
     8161 atcaactctg ctttgcaatt gtcccgtatt ggtggcggcg tagggattac tttatcaaat
     8221 cttcgtgaag ccggcgctcc tatcaaagga tatgagggtg ctgcaagtgg tgtcgttcca
     8281 gtcatgaaat tattcgaaga tagtttcagc tattctaatc aattaggtca gcgtcaagga
     8341 gctggcgtgg tttatttgaa cgttttccat ccagacatta ttgctttcct ttcagcaaaa
     8401 aaagaaaatg cagatgaaaa gattcgcgta aaaactcttt ctcttggcgt gatcgtacct
     8461 gataaatttt atgaattgac acgtaaaaac gaagatatgt atttatttag tccatatagt
     8521 gtcgaaaaag aatatggtgt cccattctct tatgtaaata ttacagctga gtatgatcat
     8581 ctagttgcaa atccaaacat tcgcaaacaa aaaatcaaag cgcgtgatct tgaaaatgaa
     8641 atctcaaaat tacaacaaga atctggctat ccttacgtaa tcaatattga tacagcgaac
     8701 cgcgagaatc caatcgatgg caagatcatc atgagtaatt tatgttcaga gattttacaa
     8761 gttcaaacgc cttctgtgat taacggcaaa caggaatatg aagtattagg aacggatatc
     8821 tcatgtaact taggctcgac aaatatcgtg aatttgatgc aaagccctaa ctttggtcaa
     8881 tctgtgcgta cgatgactcg tgcattgaca tttattacag atgcatctga tatcgatgtc
     8941 gtaccaccga tccaaaacgg aaataagctg aatcatacga ttggattagg tgccatgggc
     9001 ttacacactt atcttgcaaa agaacaaatg gaatatggct cagaggattc attagacttt
     9061 acaaatattt acttcatgct gttgaattat tggacacttg ttgaaagcaa caatatcgct
     9121 cgtgaaagaa aacaagtttt ccataatttt gaaaaatcag cttatgctga cggcacttat
     9181 ttcgaaaaat acgtaactgg agaattccag ccaaaatcag aaaaagtcaa agcattgttt
     9241 gaaggcattt ttatcccaac tgctgaagat tggaatgctt tgaaacaagc agtaatgaaa
     9301 gatggactgt atcatcaaaa tcgcttggca gtagcaccaa atggctcaat ttcatatatc
     9361 aacgatacga gtgcaagtat tcatcctatc actcgcatga tcgaagaacg ccaagagaag
     9421 aaaatcggta aaatttatta cccagcgcct tatctatcca atgagacgat tccgtattac
     9481 acttctgctt atgatatgga catgcgtaaa gtaatcgatg tttatgcaat ggcacaacaa
     9541 cacgtcgatc aaggcatgag tttgacttta ttcatgcgtt ctgaaatccc agaaggcatt
     9601 tatgaatgga agacaacaac gaagcaaacg acacgtgatt tgaatatttt aagacattac
     9661 gctttccaca aaggaatcaa atccatttat tacgtacgta cctttactga tgatgcagaa
     9721 gaaatcggaa gcaatcaatg tgaaagctgc gtgatctaat atcatagata gattcgctta
     9781 ttcatatttt aattaaagga gcattctcat gtcagaaaca tattatgaag caataaactg
     9841 gaatgaaatt gaagatatca ttgataaatc cacttgggag aagctgacag agcaattttg
     9901 gctagataca cgtatcccac tttcaaatga cctagatgat tggcgtacat tgtctcaatt
     9961 ggaaaaagat acagtaggac atgtatttgg cggtttgaca ctgctagata ctgtccaatc
    10021 tgaaagtggc atggatcagt tgcgtaaaga tgtccgcact ccccatgaag aagccgtttt
    10081 gaacaatatt caattcatgg aatccgttca tgccaaaagt tattcttcta tttttagtac
    10141 actgaatacg aaaaaagaaa tcgaagaaat ttttgactgg acaaatacga atccttatct
    10201 gcaaaaaaaa gctgagcgta tcaatgaaat ctacaaacac ggtacaccgc tagaaaagaa
    10261 aattgccagc gtctttttag aaacattctt gttctattca ggtttttata ctcctcttta
    10321 ttatttagga aacaacaaat tggctaacgt agcagagatc atcaaactaa tcatccgaga
    10381 tgaatctgta catggaacct acatcggtta caaattccaa ttaggattca acgagttacc
    10441 tgaagcagaa caagaaacgt taaaagactg gatgtacaat cttctttatg agctttacga
    10501 aaacgaagaa cgttatacag aagaattata cgatccaatc ggctggacag aagaagtcaa
    10561 gacattccta cgctataatg ccaacaaagc attgatgaac ctagggatgg atccactctt
    10621 tccagatact gccaatgatg tcaacccaat cgtaatgaac gggatttcaa caggcacaag
    10681 caaccatgac ttcttctcac aagtcggaaa cggctatttg ttaggtcatg tagaagcgat
    10741 gaaagacgac gattatttga ttggattgga ataaggattt attttaattc aaaaattttt
    10801 cgaaaaataa aaagagttta gcgtacagat attttaaatg tacactgaac tcttttttat
    10861 gcttattcac ttgtttcttc cagcactttt ggactgtcta acttttccga aacatgttcc
    10921 attcttctca ataaatagat tttccggata tttaatatta tcgtcttaat cactgcatag
    10981 acaggtatgc aaatcagcat gcctaatatt cctgcgacac ttcctgctcc aatcaatacc
    11041 aagatgatcg tcaaaggatg gatattcatt gatttcccaa ataacaacgg cttgatcaaa
    11101 tttccatcta attgttggat cactaaaatc gacaacgcca tatacaaagc ttgaaatgga
    11161 gagataaaca aaccgacaat gattgctggt accgctccaa taaaagggcc gacatacggg
    11221 ataatattcg tgattccgca aatgaccgca agcaacaagc tataaggctg atcgaatatt
    11281 ctcattgcca aaaagctcat tacaccaata atcaaagcat ctaagatcgt actgctgata
    11341 taggcagata atgtttcgtt taattctttt accgtttggc gtaactcttt acgaatcgct
    11401 ttcggaaaga agtgagacaa cgcatcaata aatttgtgcc catccttgaa cataaatagc
    11461 aaaataaatg gaacggtaaa taacaaaata aaaaattgca tcataaatga aatgattttt
    11521 gagacacttg cagataaact gactaatatg atattgaaca aattactaag agataaattt
    11581 gctgatgcta attgttcttc caaattgaaa tttttaaact cttcccgctg cgccaattca
    11641 tttaaccatt tgcttgtttc atctgcatac attggcaagc tctgggtcaa ttgaattgtc
    11701 tgttccatta actgaggcac tacattcatg acagttaaaa caactaaaat gattacgacc
    11761 aaaaacgtca ataaaaaacc gaacactctc gggatccgat gcttttcgag aaaaacaacg
    11821 attggatcga acatataata gataaaacca gcaacaagca atggtaaaaa aactgccgaa
    11881 agcattttca atattggact aaaaatattc ggcatttgta aaataaagta cacgccgatg
    11941 atcgtgagta ctagctccac tgtccagaaa aataattttg attgtttaaa acgtgattgc
    12001 atacatctcc tccaaggtcc tttgatactt ctattctatc atttaacaaa gagttacgcg
    12061 ctgagatttt catttgctta aaaaaaacaa aaaaaataaa catattttaa tgagtagacg
    12121 agtacttaaa caaaaacacg gtttggagta gtgacaaaaa ttatactgca gtcccttttt
    12181 ccaaataaac ggtgttcaaa agcttgcatc ttcgtaataa gcccagccaa acgaaaaata
    12241 taaggaacta tttttatttg gctgggctta ttacttgatg taggacatct attttacaac
    12301 ctcagatcat ttattgcgca atactgacta aatgcaactt tgtgatattt ttgtgtacgg
    12361 acaactcctc gatcacatcg gcataagtga gcccttttgg aagatcaatc gtatagatat
    12421 ttttgtacac tcggtagtca tcaacgaatt ttacgtcaaa attcacatct tcgattacga
    12481 tatttttctc ttcaaaataa tcggtaagaa aatctttggt ttcttttcta tggataaatt
    12541 ggatttctaa ctttttagtc gtaggtacat ggatcacgcg tttcactact gtgagtgcaa
    12601 tagcaatccc gataaaactg atgatcgcaa tggaatagta acccatgccg atggcgatcc
    12661 ctaatccagc aattgcccat aaagaagcgg cagtcgtaag tcccgtcacc gattgtttcg
    12721 tgacgatgat cgttcccgcg cctaagaaac cgacaccact caccacttga gcaatcagcc
    12781 ttgcttcatc tgaacgaatc gttccagcaa gctgtggata ctctaaagca tctcttaatg
    12841 caccactagc gatctcaact tggatcaatg caatgataca tgctcccata caaactaaaa
    12901 tatgcgtacg catacctgct ggacgatttt tatattgtcg ttcaaaaccg attgctccgc
    12961 caaatagcat agcgatccct aaacgtaaaa taatctctgg aattgtcaat accatatcat
    13021 tcatctaaac accctccagt ccaatatatc atgtctgaaa ggagcagaca aaaaaagtgc
    13081 aatttttacc tcttacgaaa aaaggactgt gacaaaattt ttgtcatagt cctttttatg
    13141 aataaacggt gttcaatcag ctgaccctcg gtattactca acaaacggca aaatatgggg
    13201 agctattttt cctttgtctt ctctaatact caggtcaaaa cgctgatttc acaacctctt
    13261 tagcctacta ttgaaaatta caacttactt gctgcttctt catcgatgat cacaacaaca
    13321 ttgtcgtgat tttgcaatac acttgctggc aaatgattcg ttacaggtcc ttcaatcatc
    13381 cctttgattg catcagcttt gctttcgcca aatgccatca agacgatttc tttacttttc
    13441 ataattgaac cgattcccat tgaaacagct tttgttggta catcttctgc tttatcgaag
    13501 aatcgtttgt ttgcattgat tgttgattct gttaagtcaa ctactgaagt cgttccgtca
    13561 aatgaagcac caggttcatt gaaaccgata tgtccatttt gcccgattcc taagatttgg
    13621 atatcgattg gatgtgcatc gatgatttga tcatactttt tgcatgctgc ttccagatct
    13681 tctgcttttc catctggtac aaacgtttct ttgaatggtt ttttatcaaa taattggaca
    13741 tccatgaaat gacggtagct ttgttcatct gaaccgccta gtccaacgta ctcatctaag
    13801 tttacagaag tcatttcact gaaatccaaa tcgcttgaag tcatttcttt atataaagta
    13861 actggtgtgc ttcctgttgc caatcctaat acttttgcac ctttgttcat accttctttg
    13921 atgatttcaa atgctttttt tccgccttct tcagcgtttt ttacacgaat gatttccata
    13981 gtgattgctc ctttatattt tggtatagcc caattatagc attctttttt tctttttaca
    14041 ataattagta tagaccaaat tgcatttttt tcattttctt gacaataaat aggaatccgt
    14101 gttaggtttc ataagatggt tcaatcaaac aatccatgtt gcatgaaaac caagcaagtg
    14161 attcactgtg gacgctttat gtctgcagtt tttttatgaa aaaggagtta ttatgagtat
    14221 actgacatta aacaacatta cccagcaatt tggcgacaaa atcctctatg aaggtgtcga
    14281 tctgcaatta aatgctggcg aacatcttgg gctgattgga caaaacggag ccggtaaaag
    14341 tacattgatt aaaatcatca ctggtgagat cttaccagat gacggacaga tccattggca
    14401 aaaaaatatt cacaaaggtt acctcgatca atatgtggaa gtcgatgaaa cattaacaat
    14461 cgaagaattt ctaaaaactg cgtacgcaaa agaatacgaa aaggaagaaa aaattgcttt
    14521 tctttaccaa acatatagcg agaccttcga tgattccttg ctggaaaaag ctgggaaact
    14581 ccaaacggaa ttagaccaag gagtgtttta tcaaatcgac accttagttg ctgagatgag
    14641 cagcgggttg gggatcgatg tcttaggttt agattcactg ttgaaaaatc taagtggtgg
    14701 acaacgttca aaggtgattt tagcaaaact acttttagaa aacccagatg ttctgatctt
    14761 agatgaacca accaatcact tagatgatca gcatatccaa tggctgacgc agtttcttca
    14821 ggattttgac ggtgcgtata tcgtgatttc ccatgaccaa gaatttcttg atcagatcac
    14881 cacccatatc gcagacatcg agtttggaaa gattactaag tatactggcc atctaaaaca
    14941 agcattgaaa caaaaggaac aaaatcgcga aagctatttg cgccaatatc atgctcaaca
    15001 aaaacaaatc gaaaagaccg aagcttatat tcgaaaatac aaagccggtt cccgatcaac
    15061 gatggcaaaa agccgacaaa aacaattaga taaaatcgaa cggctgacgc ctccagcttc
    15121 agcaagtaaa gctgtctttg attttccatt ctcgcctatt gtcacgacat tagcagtgga
    15181 aacaactgat ttagtcatcg gatatgatcg accgctactt gcacccatca atttgaccat
    15241 ccgctttggt gaaaatattg ccattcgagg cttcaacggg attggaaaat ctactttatt
    15301 aaaaacactg atcggtgaga ttgaaaaaat aagtggcgat ttccacttcc cagataacac
    15361 aaaaatcaat tacttttcac aagatctgta ctgggaaaat ccattagaaa caccactaca
    15421 atatttaagc agtcaatttc caaaagcgac tatcaaagaa ttacgtcgcc aactagctaa
    15481 agcagggtta gttaatcaat tagcaagtga acctctcact acattaagtg gtggggaaca
    15541 aactaaggtg aaacttgcac agttgacaat gaaccaaggt aatttactga ttctcgatga
    15601 accgacgaac catatcgatc aagaaacgaa agaaagctta caagacggga tacagaaatt
    15661 tccaggaaca gtaatcatcg tctctcatga acaagaattt tatcaagatc ttgtgcaacg
    15721 agtcattgag attgaaccaa aataaacgtt tgattgaata gttgaaaaaa aggagctgtg
    15781 acaaaagttt tgtcacaact ccttcttctg aataaatggt ggccaattag ctgaccttcg
    15841 gtattagctc aaaaaacggc aaaatatgag gagctatttt tatttggcta ttcttattac
    15901 ttgatgcaga acagcttttc acaacctctt ttcgtctatt ttttattttc gagatttctc
    15961 tttcctttaa aaacaactgt ttgaaagaag aacgcacatc cttgaaaaac aagcccgcta
    16021 accgttacac ctaaccagcc atactgctgc cacattgtcg tccctagcaa tgaaccagcc
    16081 gcacctccaa taaagtacat aaacataaat acagtattat ttcggttgct tgctgcttca
    16141 cctagccctt gtactcgtgt ttgattggcg acttgaccaa attgattccc gacgtctaat
    16201 aaaataatag ctaaaagcaa aatggccaaa tggcttccgc caaccaataa agcaataaag
    16261 cttattgttt gcataacaag tcccatatac acgatttttc tttcagaata agaatctgcc
    16321 agtcgtccca cgataggtgc agcaactgct ccagacaaac caaaaatcgc taatatccct
    16381 gcttcgaatg ttccccaatt gtaggcggga ctgctgatat aaaaaatcaa cgtagaccaa
    16441 aagatcgaaa atgttccaaa catgaaaaat ccactgactg ccgcttctct taatagacgt
    16501 tgagaagcaa ctaaatgcgg caaactttta agtgaatcaa ggtatgaaac agctgtacta
    16561 ctttgctgga cagaacgcgg cattttccag taaagcagaa gtgtaagaat tccgactgca
    16621 cataaagcaa ataaatagat catccgccaa gaaaaaagac tggcaacaat accagatacc
    16681 gtccgagaaa gcaaaatccc cacgagtaaa ccgctaagca atgtacccat gaccttccct
    16741 cttgcttttg gtcccgctag taccgcaccg taaggaatga tgatttgcgg cacgatcgaa
    16801 agtaatccta ttaaaaaaga agcgcatgca aataacgtaa acgaaggcgc aaaaaaagcg
    16861 gccaacagag agacggcaga taacgctgcc attcgaatga tcaattttgg acgatccacc
    16921 acgtccccta aaggaactaa aaacagtaac cctaacgcat agcctaattg ggtcaacatc
    16981 gtcaatatcc cgactgcact tgtgctaaca gataaactag ctgctacttt tgttccaatc
    17041 ggctggatgt aatacatatt ggcaacaaca acgccacaag tgattgataa tagtaaaata
    17101 agcgaagttg ttaaaggttt tttttcatta ctcttcattg atttccccca tttcctatct
    17161 tagtttacaa cttatggaaa agattggaaa tagaaaaata caactattcg ccacacttat
    17221 tatttgtgct tttgttttcc ttgttttttc ttgcgatctt tataaatgaa gacaactaac
    17281 cctactagca cagtaacact tgcaaccaac gtgactgcag ttttgttata tccgtgaaac
    17341 agagcttctc caccacacgc atacaacaca gacgaaggga tgactccaat cacgatcgac
    17401 aatatgattt gctgtagagg caatttcatc gcatctgcag caaaattgac catagaagat
    17461 ggaataaccg gtatcatata gcctaacatt acacctattt ttggatgttt catttgtcgg
    17521 attgcttgca cccaatgatt cgtttcggtt ttcttatcta aaaattttaa gcgatgcata
    17581 aggaaaattg cagtgatgtt accaagagca ttacccaaaa tgttgatgat cgcacctgtc
    17641 agtggtccaa agctcaaacc agcaatgact ccaactactg aagtaggaat acctggaaca
    17701 gcacacatca ccgctgtcaa tacgatcaag agtatcgatg caaaagtccc atgcgaacga
    17761 atatgtgcga gtagctctgc cttactcgct ctcggattta aaaataattg gatgtctgtc
    17821 tgatattcta aatacaaccg atagcccact accagtatca gaatgattcc caacgagatc
    17881 aatatcaatc ggattatttt ctttttcata tttcctcctg aaaaaaatct atattcatta
    17941 aataatagtt taacataaaa acaaaaaagc accgaaatcc aacataggat ctcagtgctt
    18001 ttaaatagta aaaattattt aacagttaca gatgcgccaa cttcttcaag tttagctttc
    18061 aattcttcag cttcttcttt agatacgcct tctttaactg gtgcaggagc gccatcaact
    18121 acagctttag cttctttcaa gcctaaacca gttgcttcac gaactgcttt gataacttta
    18181 actttttggt ctccagctgc agttaattct acagtgaatt ctgtttgttc ttcagcagca
    18241 gcagcgccac cagcagcagc tacagctaca ggagcagcag cagatacgcc aaattcttct
    18301 tcaatagctt taactaggtc gttaagttct aggatagttg cttcttttaa ctcagcaacg
    18361 atgttttcaa tgttcaatgc cattataatt tcctccgttt aattgttctt ttttatttaa
    18421 tgctttatgc ggcaattaag ctgcatctcc atcttctttg tctgccactg ctttgacagc
    18481 gtaagccacg ttgcggactg gcgcttgtag tacagatagc aacatagata gaagtccttc
    18541 gcgatttggc aattttgcca atgctgtgat ttcttccaat gaagagactt taccttcgat
    18601 gataccgcct ttgatttcta atgcttttgc ttctttagca aattcgtcca tgattttcgc
    18661 tggtgcgact acatcttcgt tactgaatgc aacagctgta ggtccagtga atacttcgtc
    18721 catgccttca aggccagctt ttttagctgc acgtgaaaga atagaatttt tgataacttt
    18781 catttctacg ttagcatcac gaagttgttt acgtaaacgt gttacttcat caactgttaa
    18841 tccgcggtag tcaacgacta ctactgatgc agctgctgta aatttttcgc ttacttcatc
    18901 aacgattgct gctttttttg cgataattgc ttcactcatt tttgagtttc acctcctgaa
    18961 ttttgatgga agagtttttt gcactgaaaa ggcaagtcac ttttatagtt actttgctca
    19021 accagtatta ttcatcacct attcacttcg ttcatagtga ttctactcgc tttgctcgaa
    19081 aggaaactcc tttcgctttg ctcaaccagt attgttcatc atctcttcac ttcgttcata
    19141 gtgattcaca ataaaaaact ctatgccacc gcagacatag agggactatt gaatatattc
    19201 aataaacgtc ctcggtagga aattaaggct tgatgccacc tactgtcttc ggtacaagtt
    19261 aattattaac ctcaactagc ttagcatgct aaactagttg agtcaagaat tatttttcag
    19321 ttggatcaat caacattcta atatttaatt agaaagaagc ttggtcaaca tggataccag
    19381 gtccaaatgt tgttgtgatc gttacatttt tcatgtattg acctttagct gctgatggtt
    19441 tagcttttag caatacatca tggattgctg caaagttttc aactaatttt tcatcgtcaa
    19501 atgaaacttt tccgattggt acatggatat ttccagcttt gtccacacgg taagttactt
    19561 taccagcttt cacttcgttt actgcttttg ttacgtccat tgtaactgta ccagttttag
    19621 ggtttggcat taagcctttt ggtcctaata ctcgtcctag acgacctaca gtagccatca
    19681 tatcaggtgt tgcaacaact acatcaaagt caaaccatcc gttttggatt ttttgaacca
    19741 tgtcgtcgtc acctacgaaa tctgcaccag ctgcttcagc ttcttttgct ttgtctcctt
    19801 tagcaaaaac taaaacagtt tgtgttttac cagttccgtt tggtaataca acagcaccac
    19861 ggatttgttg gtctgctttt ttagggtcta cgttcaaacg gtatgcaact tcaactgttg
    19921 catcaaattt tgcaatgttt gtttcttttg ctaaagctac tgcttcggca acaggataaa
    19981 ctttacttga atcaactttt tttaatgcat cttgcatttt tttgctcttt ttagccattt
    20041 tggttcctcc ttgattgtgg ttataacggt tgtacctccc acgtgttcta tatctttcaa
    20101 tatagagcca actgagaagc tgctgtctta gggtgtatag attattctac agtgatcccc
    20161 atgcttcgtg cagttccttc aaccatgcgc attgctgctt caacgttagc tgcgtttagg
    20221 tcttccattt ttaattcagc gatttctctt acttgatcgc tagaaacttt cgcaacttta
    20281 tttttgtttg gttcgcctga acctttttcg attttagctg cttttttcaa tagaacagca
    20341 gctggcggtg tttttgtaac gaatgtgaat gaacggtctt cgtatacaga aataacaacc
    20401 ggaatgatca aacctgcttg atcagctgta cgagcgttga attcttttgt gaatcccatg
    20461 atattgatac ccgcttgacc tagtgcagga cctactggtg gagctggagt tgctttacct
    20521 gcaggaattt gcaatttaac gattttttct actttctttg ccacgagaca tacctcctta
    20581 agtccgtgat gtggttaatt ggagatgcat atttctcctc ccacgtttta tctgatctga
    20641 aaaaatccag acaaataatg tgcaaaacaa tgcacactgg tacagtatag caaatttatt
    20701 tgttttttca agattttttt ctgctatttt tacattttgc ttgaataaac gctaaattta
    20761 ttctaaaaaa acaacaattt ttcgttctca tttcttcttt tatgttacac ttgcgctagc
    20821 atggttcaac agatgaaagc aaacaataac acggtttttt agacccttgt acaaagattc
    20881 ctacataaca aaatgaggtg gagaaaatga aagatttaag gtatagaagt gtctttgata
    20941 ttattggacc tgtgatgatt ggtccaagta gctctcacac tgccggagct gctagaatcg
    21001 ggaaaatcat acgtagtata tttggcgaac agcctgattc tgtagatatt tatctatatg
    21061 aatcatttgc aaaaacctat cgggggcacg gaacagatat tgcattagtt ggcggcctgt
    21121 tagggatgga acccgatgat gaacgtttag ctgattcttt gaaattggca tatgaagccg
    21181 gtatggaagt cttgtttgtt cctaagagtg aaaaagccga acatccaaac tctgtaaaga
    21241 tgcttctaaa aaaaggaaca cgaaagcttt cagtcactgg catttcgatc ggcggtggca
    21301 atatccagat ttctgaattg aatggattca aaatttcttt aagcatgggt actcctacct
    21361 tgatcgttgt ccaccaagat gttccaggta tgattgctaa agtgactagt atactttctg
    21421 agaccaatac gaatatcggc actatgacag tgacacggga atcaaaaggt gaaaatgcca
    21481 ttatgataat tgaagtagat gatcctcaag tagaagaaac ggtaaaaaga ctcaaacagc
    21541 tgccaaatat cgacagcgta aattatttcg attaaggagc taaaaaaatg tttttatcga
    21601 ttgaagaatt agtagatcaa gcaaaagctt atccaagtgt agcagaattg atgattgcag
    21661 ttgaaatgga aatgagtggc cgttcccgta gtcacatcat cgagatcatg gaaaaaaacc
    21721 tctcagtaat ggaacaatca atcaacgagg gaaaagaagg tgttacctcc gtcacaggaa
    21781 ttaccggtgg tgatgcaaaa aaattagatg attatttaaa tgaggcagac tttttaagtg
    21841 gagaaacaat cttaaaagct gtacgcaatg ctgtagctgt taatgaagtc aatgcaaaaa
    21901 tggggttgat ttgtgctaca ccgactgccg gaagcgcggg tgttgttcct ggtgtgctga
    21961 tggctgtccg agatcggctt gatttgacac atgaacagca attggaattc ttatttaccg
    22021 ctggcgcttt cgggttagtc atcgcaaaca acgcttctat cagtggtgca gaagggggat
    22081 gtcaagcaga agttggttct gcaagtgcaa tggcagcagc agctttggtt tgtgcaaaaa
    22141 atgggactcc tcatcaagca gcacaagcag tagccattac attgaaaaat atgatgggac
    22201 tgatttgcga tcccgttgca ggcttagttg aagtcccgtg tgtcaaacgt aatgctttag
    22261 gctcttctca agcttttatc tctgctgata tggcattagc gggtatcgaa agtgtcattc
    22321 cgccggatga agtcgttgct gcaatgtacc aagtcggacg tcaaatgccg tcgattttca
    22381 aagaaacagc agaaggtggc ttagctgtta cgccaactgc taaacgactt gcggaagaga
    22441 ttttaaataa gagggcgtga taaagctcct taatttcgag tattaagaga ttagttatct
    22501 tcataacaaa aaacgaagag aggactaatt gcccttctct tcgtttttta ttaatccatc
    22561 aatttcgtat tttttacttc atcgatcgtt tttgttcctg caagttgcat cgtgatcgac
    22621 aattctttgt tcaggtgttc aaaaacagat gttactcctt ctgccccacc tagattcaac
    22681 ccataaatca ctggtcggcc aatcgcaact aagtcagcac cacttgccag agctttgaag
    22741 acatgctctc ctcgacggac accagagtcg aaaatcactg gtacacgttt gttgactact
    22801 tcggcaattt ttggcaatac ttcaaaggac gcaggacctc catctagttg acgtccacca
    22861 tgattggata cccagatacc atctgcccct gcagagatag cgattactgc atcttctggc
    22921 gattgtattc cttttacgat aactggaaga tgggtgattt ctttgattgt tttgatgtca
    22981 tctggtgtca agccttgttt tgcagctgca taaatttctg cgattccttt tccttcgcca
    23041 ttaccacttg cactttgttc gctatacgca gctaagttag gcattggtaa tggaaattgg
    23101 aattgattga tgacgtcttc ttcacggtaa ccaccgagcg ttgaatctgc cgttaaaatg
    23161 atagctttag cccctgcttc tactgctttg ttcaaaatga actcattgaa tctgtcgtct
    23221 ttactcatat ataattggaa aaactgcggt gcgcctggcg ctgcatcggc tgcgtctttg
    23281 attgtcgtat ttgcataagt gctgatggaa aagattgatc cagcagcagc tactccttta
    23341 gctgtatcag cttccccttt ttcatgtgcc aatccttggg ccgctgatgg cgcttggata
    23401 atcggtgtat ctaattcaat tccgaaaaca gatgtatgca aatcggctga gtcgattccg
    23461 cgtaatatcc gtggcatgat tttctttgta ttaaaagaag tcgtattttc tttcatcgtc
    23521 cactcatctt cagaaccacc gcgaatatag ccaaaagctc ctgcttccat gcgctctttt
    23581 acacgacctt ctagttaggc aatgtttaca atatcgattg gatgttcttc attacttgta
    23641 tgataagtca tagtagatcc ttctttcatc ttcttgatta catataaatc atactaaact
    23701 tacaaaaagt aagcaaacat ttactctcag atacttttat cgctattctt tccaaataaa
    23761 tcgacttaca agctgatcta cttcttggtt ttcatgaagc atactatgag aggcattttc
    23821 acctgtcact actttttcct ctatttcatt tccgcgttgc ttcaacaacg catagaccgc
    23881 taatgcactg tttaacggta ctgttccatc actcaagtct gtttcatcta gttgaccagc
    23941 aatcaagaaa aatcgagtgg ttataggaat attaccgatc atttgctggt aatcttgata
    24001 gcggttagaa acgacagcag gcccctttgc taactcatct gtcagagact gcgcactatc
    24061 atcgacaaaa tcattaaaag gcgcgccaat tgctgttaat gtctgaactt gtggcaatga
    24121 cgtatcctgt ccatacgttc ctaaatagcg tagtgcacta actcctccca tggaatgccc
    24181 cactatgttg actttatcta tgccgtagtt ttctttcaga tacaaaagac aagttttgat
    24241 ccattctgct tgattccact cgttattttt attatcttca aacaatactt ggaccatcgg
    24301 attgtctgct tttccagaca aatctcctgt tgcttgtatt tgtccatctg ttcctactgt
    24361 taaaaccaat tcttttactg cttgctgatt tttttccaaa cgccgcaaca tccctttaaa
    24421 ggaattttct gtcccgctgt agccgtggaa aaaaagtgtt ggaatagtgg ttttttctat
    24481 cactttactg gattctgccg ttttttcttt atcagaaact tcagtagtat ttatcttcgt
    24541 cgtctggcaa ccacttaacg caaacaaact gataaggact aaagctgctg cagttgcacg
    24601 tttgcataaa tttttgattg gttctgtcat acacctcttc ctttccatat agaaaaaaga
    24661 aagacttgaa aggccaatca tatgatcgac atttcaagtc tctttattaa tcgattttat
    24721 ctacttgatc gaaatcaagt tctgtactgg tttcacgtcc aaacatatcg atgttgactt
    24781 tcaatttttc tttttctccg tcaacttctg ttacttgtcc ttctaatcca gcaaatgcgc
    24841 cttcgatgat tttcacgact tcgccaactt ctacttctag atcattttgt cgcaggctca
    24901 taccgataga acgcaagata tgattgactt cttctggaag taatggtgct ggtttgcttc
    24961 cggctccgtg tgaaccaacg aatcctgtca caccaggtgt gttgcggaca acataccatg
    25021 aagcgtctgt catgatcatt tcaacaagta cgtatcctgg gaatgtttta tgaacgattt
    25081 ctttttcttt cccatttttg acttcagttt ctttttcttc aggaacaacg acacggaaaa
    25141 taaagtcggc cattttcata ctttgcgcac gagattcgat atttgctttt actttgtttt
    25201 catatcctga atatgtatgc aagacatacc agtttttttc aaaagattcc attgaaggct
    25261 cctcatttca ttgtaaaata aaaaaacctc agtacacccg aagtttttct ttcgcttcat
    25321 tatagcacta aaagtacgtt gtggctagcg agttatttta aaatccagcc aaataagctt
    25381 tggatggctg tatccattac gtaaaacatc aatccgaaaa taatagaagt ttcgatgact
    25441 actaacgtat ctttacggag ttgttttctg cttggccaag ttacttgttt gatttcatct
    25501 tttacgcttt ttagaaattt catgagacaa cctcccttgc tactgtttat ttcgtttctt
    25561 tatgtaatgt atattgatta caatatttac aaaacttatt gatttccaaa cgttcgccgc
    25621 tttttccttc actgactgat tttgtgtagt tgcgagaccc acaaacggaa caagcgagtg
    25681 ctgctttttt ctttgccatt tacttctgcc cccttgctga gaatcagact tatacttcat
    25741 aacagtatca cgtaactttt tacttgtcaa tttgtgaaat aaactatcta ttgttttgga
    25801 taaaattatg atatgattaa tttgaaatta agctaaaatg agacaattgg aggtatagcg
    25861 atgctattaa aaacacttgt atataagaaa aaagatctga caaccgttac agagactagt
    25921 acattagaag aagcattaaa aattttagaa acttccggat accgttgtgt gccgatctta
    25981 gattcttcag gtaatatttt ccgtggaaac atttacaaaa tgcatatcta tcgtcataaa
    26041 gcaaatggcg gtgatatgac gcttcctgtc actcatcttt tgaaaaacgc tacaaagttc
    26101 atttacctgg atacttcttt cttcaaagta ttctttacga tcaaagagct tccttatatt
    26161 gctgtccttg acagtgaaaa tcatttttat ggtattttga cacatagtac gttgcttaat
    26221 atgctctctc aatcttggag cgtagaacaa ggaagctatg tactgacaat tgcttccaca
    26281 ggaaagcaag gagatttggc tgctattgca aaaatcattt caaaacacag cagcattgcc
    26341 agctgtatta ctcttgatat cgaaaaagac gaatacattc gacgtacatt gatcacgcta
    26401 ccggctaaca ctgaagcgaa agtatgtgaa acgatcgtca accacttaga gaaaaagaat
    26461 tataaagtgg tagaaattga aaatttgcaa aaccaagaag cataatcaaa aagcgaaaaa
    26521 agaaaacagc taatctgctt tcttttttcg ctttttataa ttagacacgc ttttcaaaat
    26581 caagcgcgcg atctaaataa tacatcaatg gtgctgaaac agccatgacg ataaactcac
    26641 ttaatgcaag cgtgccatag gttgtccaaa atgcttggcc acctgttgga actaacatat
    26701 aagcaatcag tcccatgctg atggtaaaga agatcgtgtt caatactaaa cgcatcttta
    26761 catttttcac tttgttttgc aataaagctg tcaggcctaa agaaagcaat gtttggccgc
    26821 caccaaaaac cacatccatg ataccaaaac caaagaaggc attatagacg atgaccccgc
    26881 ctaaaactcc ccaaagcaat ttacggttaa agacaactaa atgattcaaa ctttctgaaa
    26941 tacgaaactg gatcggccct gaggatacag gcgcaactaa aatagttaac gctagataaa
    27001 gagccatgat gatgccattt acaacgatta cggataaccg ttgtctgctt ttcgtttttt
    27061 gcattttctc tcctcttttc tcctagtttt tttaacgcgg gatggttgat gaaccgcggt
    27121 tatgtgaaac aaacaataat tataacagac ttttcattta tttcaatccg tcttatctgc
    27181 ttttaaaaaa gcaaaagctt aaacaatgta aacgtttgct aaaaatcgtg ggatatggta
    27241 taaagtaagt gtaaggggtc ataagaattc ccagtaaagc agacgaagta ggatctgcaa
    27301 gaacagaatt gttgtgaaac ttaaataaat aaaaaaggat gtgggaattt gagcaacctc
    27361 ttactagaga acggtctagc aagcagctga agatctaagt ggacatgccg aaccttggaa
    27421 cttctcctta cgtaagtaat ggatcatgcg cttgctaggc ttttttgtgt ttttcggtct
    27481 tcggaccttt ttcattttcc tagacttctg ctataataac caagaattaa ttttgtaaca
    27541 gatttttttg aaaaaacaag tttctttcat tcaaaaaata gaaaaagctt gctaattatt
    27601 ggttaaccag caaacaggag tattaaaata tcttgtttta ttgaatagca ggaggaaatc
    27661 atgaaattcc attatcatat attgatcaat ccatcggcag gcagcggaaa tggctataaa
    27721 gtagcagaaa gaattttacc ggtattaaaa aataagcata ttgactatac acttcattat
    27781 agtgaataca aaggacacga tgctgagatt gctgaaactt tggcaaaaga aacattgatt
    27841 ccttggatag aagaagaaca gaaaacaatc gatactttcc cccttttgat catcgtagga
    27901 ggtgacggca ccttacatca ggtacttgat actttctatc agatggaagt agaattccca
    27961 gttgcttata ttccagcagg ttctggagat gattttgctc gaggggcagg ccttccaaaa
    28021 aatcccaaaa aaggacttca gctgatttta gcagctcaat caccagaaaa agtccacata
    28081 atggcttatg aagaaaaaat aagtgaaaaa aaaggaatag ctgttaataa tttcggtata
    28141 ggattagatg cagctatcgt ccatgcgaca aatcattctt ccacaaaaaa gcgattgaac
    28201 aaatacaact taggatcatt ttcgtattta ttctctattc tccgtgcttt attcactcaa
    28261 aaaggatttc caattctggt agatgcagga ggaaaacaat taagtttttc aaacgcattt
    28321 ttatgtacag caacgaagca tccttttttt ggcggaggaa tcgcgattgt tccaactgct
    28381 gatgcgtcta aaccagtcat tgattttgtc gtggtcgaac gaatcaattt ctttaaaatc
    28441 ttgtggctca ttcttttact tatacagaaa aaacaattga aatcgaaata tttccatcac
    28501 tacacaacaa acagattacg tatcgtttca acaacaccgc aacatggtca agaagatgga
    28561 gaagaaatgg aaaaacgacc ttttgatatc acaataacca caactaaaca gttgttttgg
    28621 tgttaataaa agaggttttg tcagaagcta catctggaac accgtttatt cggaaaaaag
    28681 gactgtgaca cgacttttgt cacagtcctt tttttactat gcagttaatt ctttgcccaa
    28741 ttgggttgcc atcgatagtt tctttcttta cccaaaataa attggatcat tgaaaacacc
    28801 gcagcaaaag caactaagac acaaccattg aaccaaaaag attctgcaaa tgtttgattg
    28861 taactgagtg tttcactgat caagttatta ttttgaacaa accaatatgt tggtgtgaat
    28921 gccgctattt tgctaaccat ttctggcaag tattctgctg gtacaaacac cccaccgata
    28981 aagcaactgc ctgtgataaa aatatctttg atcccaccgc tggtttctga gttttttacc
    29041 aatgaagtga tacaagccga aaagctaatg atcggcaata aaaacaagaa agtatttaaa
    29101 atatgatacc ataccgttat atcccacgag gtgtggatca ctaacagaac cattagaagt
    29161 aagaagccac tgaacaacag cagcgagtaa ctaatcaaac taaaggttac tttgcgattc
    29221 aagtttcgtt tggtaatggg cgcactctgg tttcgtttac tgatctcttt gcgattaaat
    29281 gctagattga tgacgctgaa accgctaaaa atggatagaa acaacccata agacaagaaa
    29341 ttaaaggttg tgcccgtcaa cagctttttc atccgctgac tatatcccgc acttagctca
    29401 acttctccag taaccgagag tgccatttgt gtctgttcca tagctgcatc gattgtttta
    29461 tcttcttctt gaaacagaat cagtgtttgc aaaaaggtgt tcacttgctg tgtaacaact
    29521 gttttagaaa aggcatctgg gcgactctgt atcttgatcg tcggcatctt tcctacttcg
    29581 acttgttcgg taaagtctgc aggcagatgt aagataatgt ttacacgatt aaaataaaga
    29641 gcatcatcga tttctttttg tgaggttcca tccaatgtaa cgactgtttc cttgtcattc
    29701 aaatatttac gaaggccatc gactacaggc gactcttctt cactaatcaa ggcgattttc
    29761 gcatcttgca gagaggtatt ttcaacatca atattttgcg caaaaaagaa tgtggcaaca
    29821 acagtgataa ttacacctat cagtaaattc gatttatttg ctttaagcaa tgtccaaatc
    29881 gttttataaa gcgtcataac gcacccctct ttccatccaa gcatttccta acgcaaaaag
    29941 gaaaataaat ccgatcaacc atagaagatt ggtataaaaa ctagccaaat cagtgtagaa
    30001 ataaagttta tacaaacttt cgctgatcaa gttgacgaga tttaattgac caacaatcgg
    30061 aatatgaaga tcaatccaat acttcacttg ttccgacccc atcataccag caaaaaagct
    30121 catcgcaatc gtcactgaaa tcaagatgct atccttttgc accagattca tttttggtat
    30181 caaattacca accaatgtac ctaacaaaat cgcacacaac gaccccagag cggtaacaat
    30241 catcaaccat tgccaacgtt caccaaagtc tacttgatag ataaaacgaa agacagctaa
    30301 cataatataa atctctgtca aaacaatcgt aaagcttgct gccaaattag cgattgaaac
    30361 taacgattgc tttctaggaa tcaaggacaa gcgaatacca tttgacgatt gatttgcttg
    30421 ctgatcctgt acattgcgga ccccccacat cgtcccgtag agaatcgcca ttccaatcag
    30481 agtgaagaaa taaaaacttt tgaaactcat attacgacct gcatgaactt cttgaaaaga
    30541 tacttcttgt tcaaaaacag tcatgatggg cgcgagatca acgttctcca cttctgccac
    30601 tttactcgaa attataccaa tacgctgtaa aaattgatcc atgacgtttt tcatcaacgt
    30661 ctgactcatt cctgcttgac ccacatggag cgaaacagat gaaccatcaa tgagataaaa
    30721 accagccaaa tcatctgcat ttagcttttc attcgcttct acttccgata gttcctgtac
    30781 ttcaaataat ggtttatcat tgacttcgac ttgttctaag actgtggtaa aattttccga
    30841 aagcgctgtt tgatccgctt tgattacaat tccagtatta atcgtttcga gattgccacc
    30901 ttgatcaatc tcgccaaagg ccgccataaa catcaatcct aaaagaattg gaaaggccaa
    30961 tgtccaaaac aacatggatt tatttcgcag cagtattttt gtgcgatatt tataaaaatg
    31021 ccaaaacatt atttcccctc ctctagtctc tcaaatcctt gccagtaatc tctaaaaaga
    31081 catcattcaa ggacgcttct ttggttgcta tttttccgta actgatcttt tcttcttgta
    31141 agattgtaag aatcggcaac aatactgtat ttagttcagt agttgttact tggagctgac
    31201 cttttttata agcgattcca tcagaagcta ttgttcgctg acaacacgtt ttctgggagt
    31261 aatgggatgt cgatcgtcaa acgatccgcc gtccgtacca tatcttttag agattctttg
    31321 gttcctttag caatcacctg tccttgatcc atgatcacga tctggtcgca aagaatttct
    31381 acttcttcca tgtaatgtgt tgtatagaca atggttgctc cttgtcgatt cagctccttg
    31441 atactttcta aaattttatt ccgactttgg gggtctacag ctactgtcgg ctcatctaaa
    31501 aaaattaacc gcggcttgtg ggcaatccca catgcgatgt tcaaccgacg tttcaagcca
    31561 ccactaagtt ctcctggacg aaacgaaaca tatttttcta atcccactaa ttgaatcacc
    31621 tcttcaatca aggctttcct tttctctttt tcggaaacat ataaaccgca aaaataatcg
    31681 atattttctt gcacacttag ttcgtcaaag accgcaacgt cttgaggaac gaccccaatc
    31741 attgccttga cagcataatt ttcaggagtc attgttgtcc cgaacaattt aatggttcct
    31801 ttgtcataat ttaacaaaga aagcatacag ttgatggcag tcgatttccc actgccgttt
    31861 ggtcctaaca gacctaagac ttctccttct tgtacttgca gatcaaaatg attcaatgct
    31921 acaaaatctc catagcgttt gactaattta tcaatttcga taatcatcat gaaaaactcc
    31981 ttccaaattt cttgttgatc tgactataat aaagtgcaag aaaagaaact agtgacgatt
    32041 ggctaaacca cacatgacat ttgtcacaat gaaggagtct gctatgcata aatctcggct
    32101 actcaacgaa actagctttc tactggttat ctcattgctt ttgctggtaa gtcaaggatt
    32161 cgttgctatg caagttatct atttattgat cgccgtttta ttcactggtg tcagttattt
    32221 actgcctaca aagccaaatt ggattttcca agttattcta tttcttttat gtgccttttt
    32281 acctgatctt gtcctgtttc tgccagcgac gatgcgagtg atcgcccctg aagaaagcca
    32341 ccttgattac ttgtttttgt taggtttttt gttgatttcg acggttggta atcatccccc
    32401 catcaaggtc gcctttgtcg taattttgct gtttttttcc ttttacttac gaaaaaaaga
    32461 tcaacgaacg accgatcttg agaaaaagta cattgtctcc aaagatactc attgggaaaa
    32521 acaacaagag ttatcggaac aaaataaagc attggccctg tcacaagaaa cacaattagc
    32581 cttgcaaatt gtggaagaac ggaaccgaat tgcccgagac atccatgaca atgtcggtca
    32641 tctgctgtca agtgcgattt tacaaattgg tgcgcttgaa atgatcaatc aagaaccaaa
    32701 actaaaagaa ccattagaaa atttaagtgc caccgttcac actgggatgg atcggattcg
    32761 tcaaagtgtc catgacctcc atgagacatc gatctctttt caacactcct tagagtttct
    32821 gatcgctgat tttccttctt ctgttacgat tgaaggaaac ttgtttgaag gattgaatga
    32881 aacgcagcaa aactgcttct taatggtcat caaagaagcc ttaaccaaca gtatcaaaca
    32941 tagtcatgcc aaaaccgttg ttttacattt tcgaacgttg ccggcctttt atcgcttgca
    33001 aatcacgaat gatggcacca gcccacaaaa aaattctgct cccggtattg gtttgaaaac
    33061 gatgcgtcaa cgtgttcaag aacaaaaggg acaagtccat attttccaag aaaaccagca
    33121 atttcagtta acgatcattt taccaaggga gggacaccaa tgataacagt agtcattatt
    33181 gagatgatcc atttgtgacg aaatcgttac aaaccatttt agaaagcacg aaagagatcc
    33241 gtgtgttagg cattggtcat tgtgccaagg atgcgctagt cttatacgaa acccatcgtc
    33301 ccgatgtttt gctgacggac atccgcatgc ctaaacaaac gggcatcgat gctgccaaag
    33361 aaattttggc taaatttccc aaagccatcc ttttgctttt gacgacattc aaagatgaag
    33421 aatacatccg cgaagcattt tccatcggtg tcaaagggta tttaatcaaa cagaacttgc
    33481 aagcgattat tccttctgtc aaatccgctt ataatggaca ggccgtattt ggtaatgaaa
    33541 tcatcgagac tttcactcaa atgatgaaga ataaaccgac gattgatagt catgcttatg
    33601 cttcttttag tgaacgtgag cttgcaatca tcaaagaagt agctgccggg aaaaacaata
    33661 aagaaattgc agatgccttg tacttgagtg acggcactgt tcgcaattac atcagccaat
    33721 tactagaaaa gctagattta cgggatcgca cgcaattggc tatctatttt tatcagcatt
    33781 tgcaataagt cttatctaac acaaaaaaac ctgaatgatg ctccttcgca tcattcaggt
    33841 ttttttcaca tcttctattc aattgtcccg caaaccattt cctcacgatt gaaagaatca
    33901 aggattcgca gggctgccag gattcgttgg attgttcggc gtgttaggcg gaaaaatagg
    33961 cgccttgtct aacgtttttt ctagttttga ttcttcacga tcgtctcgtt ctacatcagg
    34021 actttcattc aatttgaatt ctttttccat ctcatctaac tttttaaagt cattgccaga
    34081 ttcttttgct aatttcgcta atttatcttc ggtttcttta gccataaagt aacgcccccc
    34141 ttttgatttc agcttagcat atcacactga aaaaacaaaa gaaaacgcta accaacgatc
    34201 atttttagct atttctcatc tttaaaatca agcatttttt gccggtataa aatcgtaggg
    34261 agaactgttt tccataccaa tcatcggatc tactaactgg ttatcgatca cttctgctgt
    34321 aagaacgaaa tctttagaaa cagcaggttc tacctcttct ttcttttctt ctccaacaaa
    34381 tgaagaaaac agatctggtt ccactgtttc cattacttgg tttttcgttt cattcgtccc
    34441 actgtcatct tctacaggta gcttttcttt tacaacccgt ggctcttttg tatagttccc
    34501 atcttcaaat ggatcatttc cgaccaaaga atcttcataa gctgctagtt tggtccggag
    34561 agtgatattc attttatcat tatccatgat ccttgatttc aacgtagtca tccggcttgc
    34621 tgattcattc gctacacaag tgacataggc ttgctcttcc cctaacaaaa tcaacagttc
    34681 cttactgcga gtcactgccg tgtagagcaa attacgctga agcatccgct gatattgatg
    34741 gaccatgggc aaaataacca tcttgaattc actgccttgt gatttatgga ttgagcaaca
    34801 ataagaaaga gtgattttat tccattcgtt tcttttataa gtgacctcat ttgcatcaaa
    34861 ctggataact aattcatcca ctttatcttc agaatctttt gccaagatga tacccgtgat
    34921 ttctcccata tctccattga atacattcaa ttcgggagta ttgactaatt ggaggacttt
    34981 gtctcctatc cgataaacgg tgtcgttcca atgtacttcc ttttttccag gtttccctgg
    35041 attgaatatc tcctgcatca ttttatttaa cgcatcgatc cctgcaggtc cccgatacat
    35101 tggtgctaat acttggatat cttgtgcacg gaatccctta tttttcgctt tttccacaat
    35161 tttagagatc aagggttcaa tttgataagc atcacttcgg aaataggaac gatctttttg
    35221 attctttgtg aaatcttttg gcagacgtcc ttctttgatc tcatgggcaa gaggaataat
    35281 actagaacca tcaccttgcc gataaatctg attgagttcc attttgggaa tttgttcgat
    35341 ttccaaaaga tcatggagta cttgaccagg accgacagag ggaagctgat ctttgtctcc
    35401 tactaagata acttgcatat tcgtcggaat cgctttgaat aatgtgttag ccagccatgt
    35461 atcgaccata gacatctcat caacaatcaa aagtccgcct tctaattctt tcgtactgac
    35521 tgaaggtgtt ttttcccgtc cattcagccc cagaagacga tggatcgtac tggcaggcag
    35581 acctgtcgtt tcgttcattc gtttagctgc tcggccagtt ggtgctgcaa gtagaatagg
    35641 aaaaatttct tgggtatatt ttgaaggatc caattccagt ccgttcaact ctgcaaaaag
    35701 attgacgatt ccgttgatca cagtagtttt ccccgtacct ggtcctcctg ttaaaataaa
    35761 tagaggggaa cggattgctt cagcgatagc ttgttcttgc gaaggtccat actgtatgcc
    35821 gaatattttt tcgatacgcc gaatattctt ctgcacttct ttttcaggat aatggatttc
    35881 ttttttacga ctgagaaggc gctggatcga attaccgatc ccccattcag aaaagtacaa
    35941 actgttttcg taaagattag tctcttcttg ttgtatcttt ccttcttcaa caagttggat
    36001 aattacagta gcaacctgat caggctggat ttcaattggg cgacttgttt ctaaaatacg
    36061 gatcgtttgc tgaagcaatg tctccgccgg tacgtatgta tctcctgttt gaactgcatg
    36121 ctgaaaaatc tgatgcaaga tagctgctcg aattcttctc ggtgaatctg ctgcaatacc
    36181 gatttgttcg gctagattat ctgcacgttt gaacccgatt ccttctatgt cttcgactag
    36241 ctggtacgga ttttcttcga tgatatccaa agactcgttc ttataggcct ggtagatact
    36301 gaaagctaac tgactaccga acccgtaacg attcagtccc atgatcactt gatccatccc
    36361 gtggtttagc cgaatcgtct cagcaagcat ttctcttttt ggcttcgtca aaccggagat
    36421 ttgttttaat accgaaggat cagcaatgat tttttcgata gcatcttctc ccagcaattc
    36481 tacaattttt tctgctgttt tcttcccgat tcctgggaat ttttcgctgg aaagatacgc
    36541 tacgatccca tttgcagttg ttggcttttc ttgctcatat ctttcgagtt taagctgcct
    36601 tccgtattta ggatgatcca ctaattctcc ataaaaacga taagtctcat tttcttgaat
    36661 atcgccgaaa cttccggtga ctacaatctc tttttcacgg taatcagctg tggtttcact
    36721 gatgtgtacc aaaagcactt tgtaaaaatt actaggattt tgaaaaaaaa tcgcggcaac
    36781 tgtaccaacg aaataaggca tttcttctgg ctgcatcgga agccgcctcc ttcttctata
    36841 gtaaaggttc tggtgctgtt tcagttccta aaaaatctgt ttggttccat actttcagtt
    36901 catatggcaa taatctgcaa ggctcttcag tctctaaaat cgtcaaacta ctattgaaca
    36961 atccacccat ttcacgcaac tcacttagct cttttcctgt catccattga atggctgcag
    37021 ttaaagaagc tccatgtcct acgaataaaa gtgggccatc tttatgttgg gcaaccgcat
    37081 ctgctacaac agtttcgata cgtgtgattg ccgattcaat cgtttctccg tgaaaaggag
    37141 aaggatcata cagatctaaa tgattacgca aattcggcag attatccgga tatttctggt
    37201 acatttcatc gatcgactga ccttctaaag tacccagccc tagttctctt agcttatctg
    37261 tataaaaaat ctctaccgga cgagcaagct ctttgttgat accttctgct gtatcacgtg
    37321 ctcgctttgc tgtactggaa taaattgctt taaaaggaac atattttacc tttttcccaa
    37381 atgctttgat ttcttcgtaa ctttgtggca aaagcggaga atcgccattg ctcccttgaa
    37441 aacgtctttc ctgattccat tgtgtcttcc catgtcgggt aaaatatagt tccatacgca
    37501 gcaaactcct gtctttcttt tattttactc tataataaac tgtctaatcc agcttgtcat
    37561 tttcatagtg atgcgtcaat tctaaattag ggaatccttg ctggcgaagt gcttcataca
    37621 ctatcattgc agctgtattc gataaattca atgagcggac atgctcatca ttcataggga
    37681 tgcgtaaaca cttttcttcg ttttcacgca taaatgtttc tggcaaaccg gttgtttctt
    37741 ttccaaataa aaagaagtgc tcttctccat cagcaaaatt ttcgtcacta tatactttat
    37801 gggcaaattt tgagatcaaa tgtagtttac gattacctaa aaatgttaaa aaagcttcta
    37861 aattactgtg gtaagtaata tttacatcat tccaataatc caaccctgcc cgttttagat
    37921 gtttgtcatc agttgaaaaa cctagtggct cgattaaatg aagcggagta ttcgtggctg
    37981 cacacgtacg ggcaatattt ccagtgtttg cagggattaa tggttcaaat aaaacgatat
    38041 gattcatcag gatcattcct ctcatattta tgttcgtttc tattttctga caatttgatt
    38101 tttgcataaa aaaaaacgta tcaactcggt gtcagtcact gcgagtcgtt acgttagtga
    38161 agctcttctc actaactttt atcagaacag gaacctgata aaaaccaatg aaaaaacaac
    38221 ttgttgctaa tataacaccc tcaacttttt tttgcaacct ctactcatta aaaactgcta
    38281 gaaaaacgtt ttctcttagt tccttttaat aattctatgt caattgtaat cttcttcaaa
    38341 ggattcttat ataagtctgc tttgattcgc tcttctacct gccattctaa aggtgacatg
    38401 gccaaaagat ctttttgatt ttcctctggt atctcaaatt cataggttac acgctgacgt
    38461 tcagttaatt tcattgcttc agcaaacttg gctactacac gttcattgga ataactctgt
    38521 ttttcaggtt gattcggata aaaagcctga cgtagttcct ttaaataatt ttcttgtggc
    38581 accaccttca acacttctcc atcttctgag agtacacgtt ggaattcttg gtaatgagaa
    38641 ggcgaaaaga tatttaaaac cgcgttgaat gtttgatcag caaacggcaa attggtcaaa
    38701 tcagctacac accaaaatgc ttcggcagat tgttctgtag ctgcgtagac tccttctttt
    38761 gaaatatcaa atccaacagc attttgaatc tttttcaatt ttgaaagttc attcaagaag
    38821 ctaccttctc cgcagccaac atctaatagc gagacttctt caggtaaatg ctgcgcaata
    38881 gtctcaataa cgggctgata catgccagat tggatcattc tacgccttgg aacgaacatt
    38941 ccttgatcgt attctgtttt aaccgcatgg gataaaaaat aaagtgttcc ttttttggaa
    39001 aggtcaaaac ggtgattatt tttgcatatc aagccattat cagacttttt tacttcttct
    39061 tggcaaaccg gacagcggaa cagctgatga ttttctgcca aaaactggcg tcctcggtca
    39121 atttttttta acatagttcc ccctcatttc tgttttatct taccgttaga tgctcacttt
    39181 ggcaactgct aaaaatatga tacaatgcct agaagaaagt gaaagataaa ggagaagatt
    39241 agatgatcaa agaattttgc gctgaaaact acacatctat tcctctagca attgctagag
    39301 gagccgatcg gatcgaattg tgtgacaact tagcggtcgg tggcacaacc ccgagtactg
    39361 gtgtgatcga agaaacgtta agttatgctg gggaaaaaga tatccccgta atgacgatta
    39421 ttcgtcctag agggggcgac ttcgtctata acgatatcga attgaaaatc atgcatactg
    39481 atttgattga agcaaagaaa ttagggacgg atggcgtagt atttggttgt cttactcctt
    39541 caggctggct agacgaagag gccttagaac tattgatcga aacagcagaa gggttgcaga
    39601 tcaccttcca tatggctttt gacagtattc ctgaagatcg gcaattcgaa gcaatcgatt
    39661 ggctggtaga acatggtgtt gaccgtatct tgactcatgg cggtcctgct gacaaaacta
    39721 ttgtagaaaa tttcccacgg cttaaagaat tgattgccta tacagatggc cgcttaatca
    39781 ttttgcctgg tggtggtgtc actgcagaga atgcagcaca tgtagctaaa gaattgaacg
    39841 taagcgaagt acacggaacc aaaatcgtgg atcttcagtt ataatcttat aaacaaaaaa
    39901 acatttgcaa caggcataat gcttattgca aatgtttttg tttttttaca taaaatagcc
    39961 cataaataaa gaagcaatat tgaaataaat cagtacactc gtcaaatcgc tcaaagtcgt
    40021 gataaaggga ccgcttgcta ctgctggatc aaatcctaga cgatccatta gcatcgggat
    40081 taaactacct gcaagattcg ccactgtgat tgcaaatagc atagccatcc ctatgacaaa
    40141 ccccaagata aaattatgtt tccaaaaacc tacgattaga aaaatcgtga tccccgtaac
    40201 agctcctgtg acaacgccgg tcaagacttc agccaaaaca agtcgaacaa atccatgatc
    40261 tttctcatca gaaatcgcaa gtcgacgaac agccacagct agcgactggg ttccagcatt
    40321 accggcagtt cctgtaatca gcgaaatgaa gactgctaaa atactcgctt cacttaccaa
    40381 gtcttcatag tgactgatta aagaagcggt cgacatacct aaaaacaaca gagtgatcag
    40441 ccaaggcaaa cgtcgagacg ctgcctttac tggattttca ttaatttctt ccacattgac
    40501 cccggcaagt cctgaataat cgctagcagc ttcatcatca ataacgtcga tgatatcatc
    40561 gactgtgacg attcctaaca aatgatcatc ataatcggta acaggaagag ctaagaagtc
    40621 ataatctcta aatgtttgtg ccacatcttc ttggtcgtcg cctacatgaa ctgaaagcac
    40681 tcgttcgctc atcagatcag aaatcattgc gtcgtcatca ttcacgatta ggtcgcgcag
    40741 agaaataacc cctactagct gaccttcttg attaacgaca tagatgtaat agattgtttc
    40801 agctacatct gcttcgttct tcagcacgta catcgcagag cgaacagttt gattggctac
    40861 gatcgaaaca aattcagttg tcatgatcgc gccagccgtt tcatcctcgt aatggagaag
    40921 ttccttgatt tcactggcat cgtctgccga catcaaactt aaatatttag caatttgctt
    40981 tttatccagc gtattaagca agtcaacagc attatctgta tacatctcag aaagcatatc
    41041 agctgcatag ctaggacgca tttcatctag gtattctttc atatgttcgt tgtcttcttc
    41101 aatcacatcg aacatatccg ctaattcttt aggcgacaga taggaataga tcagctgcct
    41161 gttctcttct gtaagagatt ggtagaattg cccctgctca tagatatgca tctctaaaaa
    41221 actgtttctg aatgcctgaa tattattttc ccttaaatct tgagtcaact gggcaaatct
    41281 ttcctctaat tcttgatttt catccaaacc aatctcacct cgccttattc ttcaaaccgt
    41341 ctcacaactg cctccatgtc tgcggctaaa ggctgcttga actccagcaa ctcttctgta
    41401 aatggatggt aaaaacgcag atcataacaa tgaagggctt gtctttttat tcccagatcc
    41461 attcgcccgc catacatatc atcaccgagc agggcacagc caattgcgga aaaatgaact
    41521 cttatttgat gtgtccttcc tgtatgcagc tggatatcaa ctaacgcgat ttctgttagt
    41581 cgcttctcta tccagtactc agtctgtgcc gaacgtcctt catcagagac cattcgcttc
    41641 aatagcgaag aataatctcg ggcgatcggc aaatcgatat gtccatgtgc cgataaatct
    41701 gaaagccttc cactgactaa ggcctgatat tttttcacaa acttcttttc acgaagttgc
    41761 atatctaatt ttgcgtgcgc aaatccatgc tttgcaaaaa gcatcaaacc agatgtatcc
    41821 cgatctagtc gcgtcaccac atggattact tggttttcgt aaccttgttt tttataataa
    41881 gcctttacac gattagccat tgtcccattt ggatggtatt gggcaggaat agacgaaacg
    41941 cctgctggtt tatttactac cagaacgtgt tcatcttcat acacgatatc gatcggtgtt
    42001 tcatccaaaa gtacggtttc atgttctcct tcagcaggaa tcacaatggt taccttatca
    42061 tccttagcta gagaaaacaa gacattttct acttgatcat taactttgat ttgaccgcct
    42121 tgaaatttta ttttcgctaa cagacccttt gagatccctt tttcttttaa aaaatatttt
    42181 acttgctgag aatgatcttt ctcataatcc catgtaaact gcatatcaat cctcactaat
    42241 aaaggcatct ttcacacgat gccaaaaatg catatgtcga taagaagcaa aatggatccg
    42301 ttcttctgct attcgataat aaattgagcg gatatttgct tttgcgacat tcaattgatc
    42361 aaccgttaca agataatctt ctgtattttc caattttatt tctagccatt catcatgggc
    42421 aatcacgatc ggagaaccca aagtacgaaa cactcgatta ttcagtgaag caatctctgc
    42481 caactgaaag gcattgatac taggatggat cacagcaccg ccgacacttt tattatatgc
    42541 tgtcgaacca gtaggcgtag aaatcgtcaa tccatctcct ctaaaacttt caaacagctc
    42601 gtttttgatg tatacatcgg ctaccatcgt acgattggcc cgtttaatcg ttgattcatt
    42661 cagtgctaaa aaatgctggt ccggtgtttc atccaaataa ctgatccgaa catccagcaa
    42721 gggataacta acactttgtt cccggttcgt tcgtaagctg tctactagtt cttccagctc
    42781 gtaatcccgc caatccgtgt aaaaccctaa atgacctgta tgcacgccca agaaacgcac
    42841 atcattcaga caatgactgt agtgatggaa cgccgacaat aatgtgccgt ctccgccgac
    42901 tgagatgacc aattctggct catgatcatc tcgttgaatc cctgcttgtt ctaaaagtgc
    42961 gttcaaacgt tctgtcactt ctatagtctt tgcttcttta ttatgaacga tcgctacttt
    43021 catcccctgc ctcctctctt tcgctttctt tcttcaaaag cgttcgatag tagacatcat
    43081 ctgattttcc tcttccatgg gagaataacc gctgtgcttc ttggatttct tcacggatac
    43141 tggacatctc ttcatccaac tgataggcag cttctgccgc acgctgaagt cgttcactca
    43201 tttcttctgg aaaaacgcct tgatatttat aattcagcga atgctcaatc gttgcccaga
    43261 aattcatcgc aagagtccga atctggattt ccgccaaaat tttcttctca ccgctgatca
    43321 gctgtacagg atactcgatc accagatgat aagaacggta gccacttgct tttttatttg
    43381 tgatgtaatc tcgttcttgg agaactttca tgtcttttct tttacggatg atctcaacaa
    43441 cttgatggat atcttctaca aactgacaca tgatccttaa cccggcaata tcagacattt
    43501 cttcttctaa acgtcccatt ggaatatgcc gtaattgtga ttttgttaca atactatcga
    43561 ctggcttgac tctcccagtg acaaattcaa tcgggacatg tcgattttgt tcacggaact
    43621 gtttgcggat cccccgcagt ttcactttca attctgatac agtttgttca tatggtgcta
    43681 aaaatgcttc ccaatctcgt tccatagctg atacctctac ttcattaaca ttcattgttt
    43741 attctaccat atatgcaaag ttttttcgta ctcttgattt atgcattgac caaaaaaaga
    43801 aaattaggca aaatagacaa aaaggagtcg atcaaatgac acaaatgctt gaaatcgaat
    43861 ataaatctat gctgacaaag caagagtacg agatattcat cgctcactat caattaacag
    43921 ataaagattt ccgtgtacag actaatattt atttcgatac cgttgatgaa cagctgaaga
    43981 aacaaaattg tggcctacgg atccgtttcg ttggtcatca agctgaatat acattaaaaa
    44041 cacctgctga aaaagggcgt ctcgaaacaa cggatacctt tataacagaa gaagcagaag
    44101 catttatctt cgaaaaacgt ctgcctcgtt caggcgcagt ttttcaaaaa ctttctgatt
    44161 tgaatatcga tcctgcttca ttaatccaaa ccggaaagct tacaacaaaa cgggctgaat
    44221 tccctattga agaaggactt ttagctattg atgaaagctg gggagacaat ctacacgatt
    44281 ttgaactgga actagaggta ggggatgcat ctgtgggaaa aattgctttt ataaattttt
    44341 taaaacgttt ttctttacct tatcgtcctg ctaagaacaa gatccaacgt atgcttgaag
    44401 ctaaaaatta gaaacctttc attttcacaa ggcacttgag ttcatttttt tacttttttc
    44461 tgttaagtta taggatgtga aagaatctag tcactagttg tatagaatct attttggggg
    44521 agattcatat gatcgaaatc tatttatttg ttaatccttt aggggaatac tgtttcgaaa
    44581 tggaacaaca gttattacaa tttatcgaag aagagtacgg gacaccatca aaagaaaaga
    44641 tgcaatttcg ctttcttcct ttggtcaatt tgcagacaat tggggatgtc atgcaacaaa
    44701 aaggaatttc tcagaacaat cttgaggaac gtaatcactt attctcaatg acttattctg
    44761 cagcattgga ctgcaaagcg gcgcagtttc aaggaaagaa aaaaggccgc cgttttttga
    44821 tcaaattgca agaagcagtc ggctgtaatg acataccata ttctcaagaa cttgctgaat
    44881 ccatatttga agaaatcggc ggcgatttga atatgtttaa agaagatcgt cagtctgact
    44941 tcgttaaaga agtattcttt tctgatcaaa aaattgctcg ggaaatgggc atcacaaaac
    45001 acccttctgc agtcgtttac aattatgcct gcgaccgtga ttttggtgtt ttagtagaag
    45061 taaaaggtac aagtagcctt tggagaatca cagagctgtg taaaacaaca gataactcat
    45121 atcaagtttt taatggtgaa catttacata caaatgattc tcagttccat tcaagaacta
    45181 gccatttaca cttgctttct aattaaaaaa atacgtcgac tcccttacaa gagagccgac
    45241 gtattttttt cgtttctatt cgagaaaatc aaaaatagaa agccttataa acgatcgatc
    45301 aattcttcta attcatttag acgttgttca aacatcgaca ttgcatcttc aatatatgca
    45361 gcctgtgtca tatcaactcc tgcttttttc attacttcca caggataatc gctgttacct
    45421 gcttttaggt aagccaagta gttctctagt gcctctggtt cctggtttag tattttcttc
    45481 gccagtgctg aagcagcaga aaagccagtc gagtattgga aaacatagta attataataa
    45541 aaatgcggta tccgtgacca ttcgaattta atttctgggt cttcttcgac tgctggacca
    45601 taatattttg cattcaattt accataacta tcacttaggt attcactagt caatggcaca
    45661 cctttttcat cttccgtatg catgaaatgc tcgaattcag cgaattgtgt ctggcggaaa
    45721 actgttcctt taaacccatc caagtaatgg ttgagtacat aagcccgtac acgagggtct
    45781 ttttctgttt ccaataaata ctccgttagg atattttcat tcgttgtcga agcaatttct
    45841 gctaaaaaga tggagtagtc gccgtacaca taaggctggt tcgaacgagt gaaataacta
    45901 tgaacactat gtcccatttc gtggacaagc gtaaatagct gatccagcgt atcatgccaa
    45961 ttcaataaaa tatatggatt tgtgtcatag cttcccgaag aataagcatc gcttcgtttc
    46021 cctttatttt cgacaacatc gatccaacgt tcagagaatg ctttttctac gatggccatg
    46081 tattcttcac ccattggttt cagtgcttct aaagcttttt cttttgcttc ttcgtacgta
    46141 aaggtaattg gagcttcccc taagaccggt gtataaagat catacatgtg cagtttttcc
    46201 acttctaata aacgtttccg taattccatg tatcgatgta acaaaggcaa atgcttgttt
    46261 accacgtcta ccaaagtatc gtatacactt tcaggaatat gattattgct caaagacgct
    46321 tctctggcag agctgtaatt acggactttc gctttaaaat tatgtccttt tatatgtgtg
    46381 cctaaagtag aagcaaatgt atttctaaat tgttcgtaaa cactgtacaa tcccttaaat
    46441 gctgcttcgc gcacccttcg atccgtgctt tctagcaact gaccatacac gccatgagat
    46501 aattggacta tttcaccatt ttctccttca atcgttggaa aaactagatc tgcattattc
    46561 aaaacagcaa atgtatcact tgaagcatca aagatttcac ctgctccagc aagaagagat
    46621 tcctgctcag cagataagac atgggctcga ttatctacta tttgctggat ataatggcga
    46681 tagacttcca attttggttc ttctttgaaa tattgccaaa tctggtcatc tgacaattgc
    46741 aatatttctg gttcaaacca ggaaacggct tcactaactt tcgaaaacag actgcttgct
    46801 cttgcataaa gcgcttggta atctgtattt ccagtgtctt gatcgttttt aaggtgcgca
    46861 tatacataaa tgacttcagt ttggcgataa accctcaata cgaattcaat cgcatttaaa
    46921 aattgagaag cgccttgatc aagtgttcct ttgtgttttt cagattgttt taactcttct
    46981 gataattcca aatatttctc atcaaactct tggtcgcttg aaaagatctt ggtcaagtcc
    47041 caagttaaat tttcaggcaa ttcttctcgt tttggcaact gctttacttc catgaaatcc
    47101 actccttttc ttcccttagc tctatctatc aaaatagatc atagtctaac atccacatgt
    47161 cacagcatct aacttttcta ctcattcatg tgtctgcttc catcaatttt agcataacta
    47221 aatcttgttt tacatagaaa accgattcct agcctatttt tatacttact attttttcaa
    47281 agaagacaag tgtatgcatt cgttaaaaaa ctgctgatag accatctctt catcaatcat
    47341 aggaaaaagc cattcccttt tatgttcttt tatttggcaa agccatgagt tgaaatcagg
    47401 aactttctgg ttttccaagg cttcttcgta caacattcga taaagaaaaa cctgttcttg
    47461 taaataaaag aaaaaccgac ttttttgata gatccacgat gatagatgca aaagatgttt
    47521 atgacgcaag tagcattgtt cttgtatgga tactgtattc ttttgcttgt gaataagttg
    47581 gcgtgtcagc catttcttcc catcttcact cactttttct atttttttca tttggttcat
    47641 tttaatttcg ttactagtga atatttcttt aaggtcccta ctagaaaaag tccagcagct
    47701 actgcaaaag gaaatatgcc ctgatacagt ctctgtaata tgatgataca aataaagctt
    47761 ttgtttcttg agatcagctt tccagcaatg cactcctctt tttctattat agtagcagca
    47821 acttttttct atcaatgaga attgcgattg tccaagaaaa gaattaccaa gtatccacca
    47881 tacttggtaa ctgttattca aataattttg ggttctttca ataaatctct ggtgagacag
    47941 tctagaacat tggatttcta ttgctagatt cgccaacaaa atatccggcc tttgccgcaa
    48001 cctcggcaaa taggcttcca aaaatactgg ttctactgac tgtcccagcc aatccatgaa
    48061 gcattccttt aactgtaagt gctcttttga ttctctttca ctaaagaccg aacagttatc
    48121 tgatttttga tgggcaaaat gagcacattt tttctttccc tttttcaaaa tcactctttt
    48181 tcgacagcca cgacaataat atttctttcc tttttctgca tgtttagcaa aacagcactt
    48241 attatattca ttcattgcaa gcagcatttt tctccctctg aacttattta cgtctaagaa
    48301 tttatttaca cgaaaaaaga gggcgtgaaa aagctattct gcatcaagta ataagaacag
    48361 ctaaacaaaa atagttccca atatttttcg tttggttggg cttgttaccg cagatgcaag
    48421 cttttgaaca catttatacg aaaaaaagac tgcgacacaa tttttgtcac agtctcttac
    48481 tataatgctg atttatacaa aataaaattt cgttcgctcg atagcattac gcgccatcag
    48541 acattgtcca tattcattca atgtttctgg tgtacgatcg ctcttttcag caaactccaa
    48601 aattcgagca atctgattct ctacatccgc ttggttgaga ttttccatcc agaagtgaac
    48661 gactaggtaa tagcgctctt cgaataaata caaatcagtc caagcagact ctagttcgac
    48721 atctgttgca agttggatca cttgttcaaa atcttcaaaa gcaaaagttg cttgtctttc
    48781 ctgttcttct agggcgcttc cagataattc ttcctccgga tcgctgccaa taatctgctg
    48841 acgaagaaag tcactcacat cttctgaatc aacagtagaa tcttctgatt gagaaatttc
    48901 ttcatccgga agatttttac tgataaacag ttctaatccg tctccttttg gcaaaacttg
    48961 gaaagtgaca gcttcgcttc ctcggaactc ctcttcgata tcaacttctt ccaaaatact
    49021 ataaaagaaa ttttcgattt ctttgtgatt gccaagtaaa tccaaaaatg aaacgccccg
    49081 ttcctctaaa tcttcatgac caattagtac acggatcgta ttttcattaa tatgttccat
    49141 ttccattttg ctacacctca ctttgccaca atgtttaata tagattcatt gtagcgaaat
    49201 cagcttaaat gtaaagaaaa aaaatctcgc tgttaagcga atttaataaa accagcggat
    49261 ttttcaacct ttcaagctat cttatttaac tataacattt ttggcagacg aatactatat
    49321 agccgacttt tcagctaatt ttccctctct atttttcgtg caatcatcat cttctactac
    49381 ctttttatac atctaatcaa gaatactttt tgtaaaaaca aaaaaacttg gaaaaactat
    49441 ctttgt
//
LOCUS       DAZUKC010000011        47754 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000011 DAZUKC010000000
VERSION     DAZUKC010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.011"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..1188)
                     /locus_tag="ACG80T_000586"
     CDS             complement(37..1188)
                     /locus_tag="ACG80T_000586"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFS4840262.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1201..1638)
                     /locus_tag="ACG80T_000587"
     CDS             complement(1201..1638)
                     /locus_tag="ACG80T_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4840263.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1653..3419)
                     /locus_tag="ACG80T_000588"
     CDS             complement(1653..3419)
                     /locus_tag="ACG80T_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFS4840264.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3683..4360
                     /locus_tag="ACG80T_000589"
     CDS             3683..4360
                     /locus_tag="ACG80T_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS4840265.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4363..5490
                     /locus_tag="ACG80T_000590"
     CDS             4363..5490
                     /locus_tag="ACG80T_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4840266.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5690..6130)
                     /locus_tag="ACG80T_000591"
     CDS             complement(5690..6130)
                     /locus_tag="ACG80T_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4840267.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6141..8174)
                     /locus_tag="ACG80T_000592"
     CDS             complement(6141..8174)
                     /locus_tag="ACG80T_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFS4840268.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8176..9879)
                     /locus_tag="ACG80T_000593"
     CDS             complement(8176..9879)
                     /locus_tag="ACG80T_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4840269.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9973..10923)
                     /gene="manA"
                     /locus_tag="ACG80T_000594"
     CDS             complement(9973..10923)
                     /gene="manA"
                     /locus_tag="ACG80T_000594"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFS4840270.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10962..11987)
                     /locus_tag="ACG80T_000595"
     CDS             complement(10962..11987)
                     /locus_tag="ACG80T_000595"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFS4840271.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12196..13065)
                     /locus_tag="ACG80T_000596"
     CDS             complement(12196..13065)
                     /locus_tag="ACG80T_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4840272.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13524..15416)
                     /locus_tag="ACG80T_000597"
     CDS             complement(13524..15416)
                     /locus_tag="ACG80T_000597"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4840273.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15413..17140)
                     /locus_tag="ACG80T_000598"
     CDS             complement(15413..17140)
                     /locus_tag="ACG80T_000598"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4840274.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17156..17806)
                     /locus_tag="ACG80T_000599"
     CDS             complement(17156..17806)
                     /locus_tag="ACG80T_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFS4840275.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18010..18306
                     /locus_tag="ACG80T_000600"
     CDS             18010..18306
                     /locus_tag="ACG80T_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS4840276.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18478..19098
                     /locus_tag="ACG80T_000601"
     CDS             18478..19098
                     /locus_tag="ACG80T_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFS4840277.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19190..20356)
                     /locus_tag="ACG80T_000602"
     CDS             complement(19190..20356)
                     /locus_tag="ACG80T_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS4840278.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20368..20721)
                     /locus_tag="ACG80T_000603"
     CDS             complement(20368..20721)
                     /locus_tag="ACG80T_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFS4840279.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20849..21871
                     /locus_tag="ACG80T_000604"
     CDS             20849..21871
                     /locus_tag="ACG80T_000604"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFS4840280.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            21890..22051
                     /locus_tag="ACG80T_000605"
     CDS             21890..22051
                     /locus_tag="ACG80T_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840281.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22310..22744)
                     /locus_tag="ACG80T_000606"
     CDS             complement(22310..22744)
                     /locus_tag="ACG80T_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840282.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22854..24713)
                     /locus_tag="ACG80T_000607"
     CDS             complement(22854..24713)
                     /locus_tag="ACG80T_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS4840283.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            24714..24854
                     /locus_tag="ACG80T_000608"
     CDS             24714..24854
                     /locus_tag="ACG80T_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFS4840284.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(24831..25991)
                     /locus_tag="ACG80T_000609"
     CDS             complement(24831..25991)
                     /locus_tag="ACG80T_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS4840285.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26298..27722)
                     /gene="araA"
                     /locus_tag="ACG80T_000610"
     CDS             complement(26298..27722)
                     /gene="araA"
                     /locus_tag="ACG80T_000610"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFS4840286.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27738..28436)
                     /locus_tag="ACG80T_000611"
     CDS             complement(27738..28436)
                     /locus_tag="ACG80T_000611"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFS4840287.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28393..30054)
                     /locus_tag="ACG80T_000612"
     CDS             complement(28393..30054)
                     /locus_tag="ACG80T_000612"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFS4840288.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30272..31672
                     /locus_tag="ACG80T_000613"
     CDS             30272..31672
                     /locus_tag="ACG80T_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFS4840289.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31924..33003)
                     /locus_tag="ACG80T_000614"
     CDS             complement(31924..33003)
                     /locus_tag="ACG80T_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4840290.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33089..34048)
                     /locus_tag="ACG80T_000615"
     CDS             complement(33089..34048)
                     /locus_tag="ACG80T_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFS4840291.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34060..35571)
                     /locus_tag="ACG80T_000616"
     CDS             complement(34060..35571)
                     /locus_tag="ACG80T_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFS4840292.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35593..36156)
                     /locus_tag="ACG80T_000617"
     CDS             complement(35593..36156)
                     /locus_tag="ACG80T_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS4840293.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36162..37025)
                     /locus_tag="ACG80T_000618"
     CDS             complement(36162..37025)
                     /locus_tag="ACG80T_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4840294.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37039..37935)
                     /locus_tag="ACG80T_000619"
     CDS             complement(37039..37935)
                     /locus_tag="ACG80T_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4840295.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38110..39399)
                     /locus_tag="ACG80T_000620"
     CDS             complement(38110..39399)
                     /locus_tag="ACG80T_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4840296.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39371..40888)
                     /locus_tag="ACG80T_000621"
     CDS             complement(39371..40888)
                     /locus_tag="ACG80T_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFS4840297.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            41086..41976
                     /locus_tag="ACG80T_000622"
     CDS             41086..41976
                     /locus_tag="ACG80T_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFS4840298.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(42049..42873)
                     /gene="nadE"
                     /locus_tag="ACG80T_000623"
     CDS             complement(42049..42873)
                     /gene="nadE"
                     /locus_tag="ACG80T_000623"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFS4840299.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(42885..44357)
                     /locus_tag="ACG80T_000624"
     CDS             complement(42885..44357)
                     /locus_tag="ACG80T_000624"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFS4840300.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(44994..45356)
                     /locus_tag="ACG80T_000625"
     CDS             complement(44994..45356)
                     /locus_tag="ACG80T_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFS4840301.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            45579..46331
                     /locus_tag="ACG80T_000626"
     CDS             45579..46331
                     /locus_tag="ACG80T_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS4840302.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(46471..47394)
                     /locus_tag="ACG80T_000627"
     CDS             complement(46471..47394)
                     /locus_tag="ACG80T_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4840303.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(47409..47681)
                     /locus_tag="ACG80T_000628"
     CDS             complement(47409..47681)
                     /locus_tag="ACG80T_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840304.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        13894 a         9933 c         8250 g        15677 t
ORIGIN      
        1 aaaatttcgt cacattcccc tttttcgtat cctattttaa gcgacttgct tggcataacg
       61 gttcacgttt tgcttgatat tcccgattgt tttcggatca ttgattcctg tgacttcttg
      121 aatcaattgt tccaagtttt catggatctt catttcatgc aattgacggc tttgttcatc
      181 ttcaggatca tagtaattaa aaataatccc cattgttgcc gtcaaatgtg ggcaagtcaa
      241 gttatgttct tgtagttctc tcactggacg tgtaaatctt tcttgataac ctaacttacg
      301 taatggtgtc cgagcgacac gtgtgatcgc atcagagata tttttgtttt ggaagcgatg
      361 aatgattttt tcaatatacg catgatgctg ctctgcatca aacccccatt tggcaatcag
      421 caaacttcct gtttcctgca aaactgattt taattgtgcc acaactaacg catcttgcat
      481 tgcttcatca atggtctggt aacctaacaa ggcacctgta taagcgacag tcgcatgacc
      541 agtattgaca ctaaatagtt tgcgttcgat atatggttct agatcatcta catataaaac
      601 cccttttaat tggatttttt tattttttct ttggctatcg tcaatgaccc attctttgaa
      661 aggttccact tggacgaata aaggatcttc atggtgttgt aatgggacga ttcgatcgac
      721 tgctgcattt ggaaagccga tatatttgtc agtaaatgat gtttccggaa gatatttata
      781 cacttctttt tctagaaatg ctgaaccgcc aatcatattt tcacaggcaa tgatatctaa
      841 agatgttgtg cttccttcct tttctctttc ttggatacct tttgcaatca actcagcaat
      901 aaaaggtaag atattcgggc caatggcggt agtaactaaa tctgcttcag cgatttcttg
      961 gattactttt tctggatttg tttgattgtt gattcctttg acatttttta cagtgatttt
     1021 ttcttttgat tcatctgcta attcgatttc gtattctttt ctttcattta aagcatcaat
     1081 gatcgtgtga ttcacatcta cgaaagtaat ctcaaatcca ttagctgcca aaacctctcc
     1141 aataaatcct cgaccgatat ttcctgcacc aaaatgtact gctctcattt ttctctctcc
     1201 ttaagcaatt gctagatttt ccgttacttc ttctttgctg agcgcgtctg ctagctggac
     1261 aacattgtcc atatcactgc aataaagtgc gatttgctgt acaagttgca aatgctcatc
     1321 tccgactcct gcaataccga acaaaacagt cgcaatcttt tcttcttctt cggtaccaaa
     1381 atctacacca tctggtactt gaacgacaca gatgccggat tttttcacat atttttttgc
     1441 ttcatctgtt ccatgaggaa tcgcgataaa attccccata taaacagata acattttgtc
     1501 tctatctacc atggcatcga tatattcctc atcaacgcac cctgcttcta caagcatctg
     1561 accgcaatag cggatcgctt cttctttggt tttaaaagat cgattgagct cgatcatgtc
     1621 gaatggcaaa ctttgcatgc tgttttcctc ccttaagctg tctgtttcaa atattgtgca
     1681 atcgattcgt atttttgcgg attgaccatg ctgttgactg ataaatgaat ggattgaggt
     1741 gcagccatct gtactttttc cgctaaactt tctttggcta cgatcagtga atcaggttca
     1801 tcttttaaat cttggatatt gacttttctt aacgggatag ttaaatggtt cttgtctaat
     1861 atatctttga gcacggtcat ccccattgtt gctgatccgg aacgatcatc gtggacaagt
     1921 agaatctctt tgatttgttt gatgtttgct gaaagaaact cttctgctgg ctcattctca
     1981 atttcagaaa ctgatgtttt ctgcttgctg cttaattctt gaatgatttc atcatatcga
     2041 ggtgaattca aaaagttttc tactgagaca aaacgcgcgc tcggtgcttt ttgttttgct
     2101 ctttcttgta gttcgacctg agtaacgatc aatgtattcg agtcatctgt cagttggctg
     2161 attgcttgat tggtcaccgt ttgaggcaag tctgcttcct gtactttttt tctcaagatc
     2221 gaagcgccca tcgcacttga tcccattcct gcatcacaag caaaaatgat tttttgtacg
     2281 ccagagaatg tagctccttg aggaatacct tgggcacctt tggaagcttg cttagctgcc
     2341 tttgtctctt gaacagattt ttcgaagtct tcatttgtat ctttcgcatc cgctttcaaa
     2401 attactgcag aaataccaaa cgaaacagct gcggcaacaa tcacgcccaa aataacagga
     2461 aggtaagaat tgaacggtgt catcgctaga atcgcaatga tcgaccctgg agaagctggc
     2521 gctcttagtc cagcatccat caattggaaa atgaaacttc ctgacattcc tccgcctata
     2581 actgataaga ataataacgg tttcatcata acgtaaggaa aatagatttc atggatcccg
     2641 cctaaaaagt gaatgatcat ggctcctggt gctgaagatt ttgctgctcc tttgccaaac
     2701 aaagtaaaag caagtaatac gccaagtcct ggaccaggat ttgcttcaag caagaataga
     2761 atagattttc cagcctctgc tacttgttca gttcccaatg gtgtcaaaat cccatgattg
     2821 attgcattat tcaaaaataa gatttttgct ggttcaataa aaatacttgt caatggaatc
     2881 aaatgggcag ataaaatgat ctctacacct tttgccattg cttgagtcaa tgcatcgaca
     2941 actggaccaa tcgcagaaaa acctaataac gctaatccaa aaccgataag acctgctgaa
     3001 aaattattga ctagcatttc aaaaccggat ttgatccgtt tttggaatag ctgatcaaat
     3061 ttcttgatgg catatccacc aagaggtccc atgatcatcg cgcctagcat cattggaata
     3121 tctgttccga caatcacacc catcgttgca attgccccaa caactgatcc gcggtcacca
     3181 gcaattactt ttccgccagt ataaccgatc aataaaggaa tcagataagt tagcattggg
     3241 cttaccatgg tagccaaagc ctcatttggt aaataaccat ctgggataaa tagagccgtt
     3301 aatacacccc aggcaatcag tgctccaata tttggcatga ccatgcttga taaagtactg
     3361 cctaattttt gtaccctcgc ttttagcgaa atgttgtttt tgggaatcgt ccgttccatt
     3421 ttgtctctcc ctctctcctt ttgatactct aattgtatcc gcttactaaa aagagaaata
     3481 gtcgtacttt ggcacgaaac tttgtgccaa aggtttgagt atgtcagcaa aataaaaaga
     3541 tttcggtatc agaaaacttc aattttaaat aaaactgtta ttttaatttt tttataatat
     3601 gcaaacaata acttttgtta gcatttaatt tagatgtttt ccattctatt atgttattaa
     3661 attttatatt ggaggcctat ctatgacatt actgatcaaa aacatgaaca tgaaacgtgg
     3721 taaaaaacgt gtgattcgac aattgaatct tttagtagaa tcaggagaag tagctgcttt
     3781 gattgccccg aacggatttg gcaaaacaac tttgctgaat ggcattgccc aactgctgcc
     3841 aacaacttat gatcaattgt cccttcacaa catctctcct actgcttata cagattatcg
     3901 ccggtctttc ttttttctag aatcaacgaa tcagttatcc gactatctat cggctgttga
     3961 ttatttgact ttgatcaaat actattggca gagcaaagta gaatgccaaa ctgttttcca
     4021 acgattgaag atgactgagt accttacttt gccgattgga aaaatgtctc taggaatgaa
     4081 acaacatgtc ttgcttgctg cttacttgat cagtgattca tctgtcatgt tattcgatga
     4141 accgttgaac ggattagacc caggaagtat tgatatcttg aatagcctca tcagacaatg
     4201 gcagaaagag gggaagacga ttcttctttc ttcccacgct atcgcgaata tccaatcgat
     4261 ttgttctaaa gcatgttttc taaaaaatgg agaaatcgtc acagaatcct caaatatgga
     4321 agagatttta gctatttatc gtaaattata tgaggtgtaa ctatgtcttt acttacattt
     4381 gaacgcaatc tcttactaaa aaacaaagta aactttttat ttcctctatt gttggtcgtg
     4441 ctctttgcat tcccactttt ttttgatcat aaacttgctt atacagaatt cgatgaactg
     4501 aatcataact atgaagagat gcaacgtttg attgaaacgt tgaaagaaga tgagaatgag
     4561 aaagaatttg tggaaagttt agagaaaagc aacaaactga tcgaagcaat cttacatgca
     4621 aaaaacacag ggaatgtcca gcaaatggtc gaagcaactt atcattatga aaaagatata
     4681 ctggatcgac tgatttctgg tcaaagacaa ggaattccaa tcattgaaca acaaaaacgt
     4741 gtcgagttgc tccgttatat gaaagaacat cagatccaaa gatattctat ttttgacttg
     4801 ccagcgcatc tccctttagc aaattactac gaaaatattt tttcaggaat gatttctagt
     4861 tttttgattt tatgtataac cgccttgttt ctttcttcca tcatctctta tgaaaaaaga
     4921 aagcaggtaa tctctctagt caatctatta ccggatagta tggtaaagaa acatagcatc
     4981 cgttttacca tttattatgg tgcagctatg ctaagtctcg tgatgccatt tttgatcgtc
     5041 tctatactag taataatcaa aaatggtttg ggagacttcc gttatcctgt aggaacgatc
     5101 attggtcagg agatacgaat ccttccgatg tatgagtatc ttttccagag ctttctgttc
     5161 ctgttgttgt gggtgctctt tttaagtaca atcagttttc ttctatctgc tttatttgaa
     5221 cattctctag tcaatctttt aggtacactt ctttgtcttt tcttagcaga gtatcgcttg
     5281 ttttcttcga ttggatggat agaatcaatc agtcattact tgccaacttc ttatgttgat
     5341 tttcaaaatg tgatcatagg aggagatatt ttctcgccac tagcttcaga acaggttact
     5401 ttcatgaatg gtatcttgac acttggtata tggagtatcg ttttattatt cataggtatg
     5461 ggcactatct atataaaaaa gagctattaa aacacaaaaa taataggggc ggtgacaaaa
     5521 accttgtcac cgtcccctat ttcaaataaa cggttttcta gaataaatag ttttcaaaag
     5581 cttgcatccg cagtaataag aacctcccaa caaaaatagc tcctcttatt tttatcttgg
     5641 ttgaagttat tacttgatgc aaggcagctt tttttactgt gaacattggt tatatatgtc
     5701 catgttcaaa aatcatttgg acttcttcaa tggttttcgc atcactgagc gccattactt
     5761 ggtcgatgtc tgaacagaaa aaggctaaat cttggagcac cgctaactga tgttcttttt
     5821 ttaaagccac tacaaataag agtgtcgcga tttttggttc ctcttttgtt ccaaagtcca
     5881 caccatcagg cacttggact aaatagattc cttcttctac tacttcttca tctttgtcag
     5941 tcccatgtgg caaggctacg aaattcccga tatagacact cgctttttct tgtctttcga
     6001 tcattgattg gatatatgcc ggtttgattc ccccttgttg ggtcatccac tggccgatct
     6061 cggtcaacgc ttctttccaa ttttgatagg attgatcgag caatattttt tctgctggta
     6121 gctgtatcat cttttcacct tcatttcttt atttcttcaa tcaggatttt cgctaacaac
     6181 tgataaacga tttctttatt tcctgagcgg aagatctcgg tgttcaagtc attcatgatg
     6241 attgctccac tgatctttcc taaaagaatc ccgtcttttt cttggattgg cgaaggcgcc
     6301 agcataacta acatacggga caaatcgact ggctgtctat ccatcccttc aatggtcaac
     6361 ggatgtttca aattgaaaat acaaaagatc ggtttagaaa tcaatgagct ggatgtatgg
     6421 aataacccca tatttgtatg aggaataccg ataggtgctt gttcatatcg cttaagcagc
     6481 cggcgatgaa ccacctcttc atccgttact agtccttcag gaaaataagg aaataagctg
     6541 cgaatcgttt cggaaatcgt ctcaggatta tccagttctt ggataaaaaa acgaccgagt
     6601 aattgattga tttgatccat gaaatgcatt gtttcctcat aagtatcttc tatcatccac
     6661 tcgttattgc tgttatacgt aattttttgc ctttttttct tctcaatatg cgaaaaagct
     6721 tcttttagct gcttgacttc atcttctagc agtagcgggg agactaaaat atattttcca
     6781 ccgtatcctg gtaaaataga tgtagaaata acaacatcat attcttcttg taaatcgatt
     6841 ttttttaaat cagcaatccg atagaaatga atttgttgga tgaagggaaa ataccttttc
     6901 aatcgtagtt ccaacatgct agtagaagct aatccacttg gcgaaaatcc tgcaatactg
     6961 atattgcttt ctttcggact tcgttccaat gaattcgcaa aatgaaggac catataagcg
     7021 atctcttctt cggataattt tttttcttga aaaatccgct ctactgcttt acggattgca
     7081 ttcgcaactt cctcgtattg aaccatgatt ttttctaaaa tcggattggt cagtgtctct
     7141 tcttgtaaaa cggctcttga aaacacacca gataaatgag tcaacagcat tttgtacaaa
     7201 gtttcatctt cataaaaatg gacattcgtc tcttcactta cttcttgaat cagttgtttt
     7261 actaaataag caagctgtgt atcgaacgtc tcttcaaaga aattctgatc aaatgagttc
     7321 gtgaagtcat ttaataatcc tgcaaaaaag acgatctcat ttactggata aagttgtttt
     7381 gtctcagaag cgatccgtga aaacaatcgt ttagctgtct gtagtaattc tttgtttgcc
     7441 agactattat atgtctcatt tgtcagtaaa tgatttttag tcacacgctc catcgtcaat
     7501 gtcagcatca aaaccaaatg ttcaagcttt ctatcactta gattggtctt tttttggatc
     7561 aattcttccc cgattacttt gcgtgcaaga gatagatgtt gcggatcaac gaatcgaaaa
     7621 aaataattca gtgatgcatc gaaagaaata ctatgaaaaa gttcatattc attgatttct
     7681 aattctaata cattcgctgt cagaagccgg cggtattttt ccggtccttc gatctcgtat
     7741 ccctggttcc gaatgatttc caaaggcagt tggcgaatac tttcttccaa ctgtttgata
     7801 tctgcataaa aagtggtatt actgatgcga tatgattcca tgaaatgagc aagactcaac
     7861 ggctctgtcg tcagtaacaa ctcggctaaa atcgcgtgtt gtcgtttagc agtggataag
     7921 tcattacttt tcccatctgt attttctttt aattgctcta atacttcttc tgatgcctca
     7981 atgacaaatg agcctctagc tgcttttttc aaagaagcat ttaccgattt caatgagact
     8041 tcaagattat ctaattcgcg gtaaatagtc cgtttactga ccttcaataa attcatcatt
     8101 ttttggatag atacaggtga cgggctactg agaaattctg ctaataattg tttttctctg
     8161 ggcgataaat acatcttata tttctccctt cacgagtttt tcgtattcat tcgtagctac
     8221 ccaatgttca actagaacta gccgagggat cttttcttct agctgttcag ttacagctag
     8281 ttctctttgt gcgatataaa tcgtttcttc ttggattttg attttttcta ttgattcttt
     8341 tgagataaca aactgttttt tcgctttttt tgctaattga cttaaaatcg tcatgcccat
     8401 agtttgtgag ccgcgtctgt tgtttttata caaaaataca atattagcag gcgacttttt
     8461 ttgttctgtg atatctgcta tctctttttt ttcttcccgc tttggaaatt ggatcgcttc
     8521 ttttattcgt gacagcaaga tatctgtttc caaaaagtga tccagtacat agatttgact
     8581 ttgaggtact ttttctcttg caagacccgc tagttcatgt tgcacgataa ccagctgatt
     8641 tacttggtta ttcaattcat agatagagct gtatccgaca gaaagatcga ttttttctgc
     8701 tgctaatttc tttttcagca gggcggctcc catagcactt gatcccatac ctgagtcgca
     8761 agcaaaaatg acggaatgaa tgatagatgg ttccaagatt tctccccttt ctttgaccgt
     8821 tttgttctct ttttctttat ttggtaattt caatagtatc gctgcacaaa tgaaggtcac
     8881 aaatgtactg attgctatcc ctatacttac cccagctagt tgtggataag gcgcattggc
     8941 aaaaatcacg agtagtgacc caggcgatac aggtgcagct aatcccgtat ctaaccattg
     9001 aaatatcaac gtgccgcttg ccccaccagc cattacagct aaaaatagat acggtcgcag
     9061 caagacaaat ggaaaataga tttcgtgaat cccgccaatc gcatggatca tcaatgctcc
     9121 actcgcattt actttttcct gtttttctgc aaaacaaagg atagaaagta agatccctaa
     9181 accaggtcca ggatttgcct ccatcaaaaa taaggaagag tacccgactg tttgtgagaa
     9241 ttcgattcct aaaggtgtca atacaccatg attgattgca ttattgaaaa atagtacttt
     9301 tagaggttct aagatcagac tgacaagtgg taacagattc attgtgacta gccaagacac
     9361 aacggaggcg agcaacaagt gaacactatt taaaactggt tcaataaaaa cgattccaat
     9421 tatacaaata acgctaccag tcagtcctac tagtaggttg ctgacaagca tttcatatcc
     9481 agccttgaat ttcacgtgga atctagaaac aaattgttca tatacgatgc cggcaagcgg
     9541 cccgataatc atcgctccta cgatttgtgg agcattggaa cgagcgatca accctaaaga
     9601 agcaattgca ccggcgactg cccctttttg tccccctacc gatttcccgc ctgaataact
     9661 gatcaagatg ggtaaaagca gctggatcat ccaatggcat acctcttcta atgtggtgtg
     9721 caaatcgcca gacacacctt ttgttgctaa tgacaagatt ccccaagcga taaacagact
     9781 tagatttggc atgatcattt catttaagtg atgtccagct tttctcagtt gtttcataga
     9841 cacgcgactg ctgttgtttg ctttccattt gtattccatc tttttcacca tacttctatt
     9901 tattgatgga cggattttat gtagaaaaag cagcaaacga aaaaccgtct gctgctttca
     9961 gctatctggg aactatttgc ctggttcagt cgcaatgatt gctagttcac cttggaccgc
    10021 ccactcagaa acgccatttg gaataataca gcttgttcct ttttctaatg gatatgtttt
    10081 tccgtcaacg acaaggtctc ctgctccttc gattaccgtc atcaatgtat aaggggcttg
    10141 ttttttgaaa gatgtgattc ctttaatatc ccactcatat acattgaaaa actcagtttc
    10201 taaataagtg acgatcgatg aattcccttt tcggacttct ttgatttgta attctggtgt
    10261 tttcgcaggg acagttgtta catcgatcga ttgttggata tgcagctctc ttgtttgtcc
    10321 ttgcgcatct tttctgtcgt agtcatacac gcggtaagtc gtgtcagaac tttgctgtgt
    10381 ttctaaaatc atgattcctt taccaatagc atggatcgtt ccgcttggta cataaaagaa
    10441 atcacctttt ttaactggta ctttcttcaa aaggtcatcc cagcgcccat ctttgatcat
    10501 ctcagctagt tcttctcttg tctttgcatg atgaccatag atgatttctg caccaggttc
    10561 agcatcgatg atataccagc attcagtttt tcctaattca ccttcatgtt tcatcccata
    10621 ggcatcatct ggatgtactt gtacagatag atcatcttct gcgtctaaga tcttgatcaa
    10681 taatggaaac actggttctg aaggatggcc aaacaattcc ttgtgttctg accatagttc
    10741 atctaatctt ttccctttga attcgccatt ttctactaca ctgacaccat gaggatgcgc
    10801 actgatcgcc caatcttctc cgattttatc gttagggata tcaaaaccaa agactgaacg
    10861 taaacgactt cctccccaaa ttttttcttg aaaaactggt tttaaaaaca taggttcctg
    10921 cataattcaa actcctctca tttttcatag ctattatagc attatttcgg taataccgct
    10981 ttctctttca aaaagttttc aacaatttca tctcgacata tgagatactc cgcacgatga
    11041 tcgacaggat gtacgcgatt tgacaaaaat atcaacgctt cttgtgtttt tatatcaatc
    11101 aagaaaaacg tacctgtata accagtatga aaaagaatcg catgaccgtc ttctacatcc
    11161 ttttttaagt cccatcctaa agaacgatag ccattttttt tcggtgtttg atcttgcagt
    11221 aaagaatcaa tcgtctcttc ttctaaaaaa cgaccattac ggatttttcc cttgttcaaa
    11281 tacatttgga caaaagtaat gctgtctttc aaatcaataa agagacccgc gttacctgca
    11341 tgttcagcca aaactcttgc tttcggatca tgagtttgtc cacgcaaaac ctcacccgaa
    11401 ggcagttgtt ctgtgggaac gatttttttg gaaggaaaag gaagaaataa gctgttcatc
    11461 attcctaatg gttccaatac ttgttcttga aagatttcca ctgcagtttc ttggtaaatt
    11521 tcctctagca tcaacccaag aagaatcgta ccggcatctg tatattttac ttcttttcct
    11581 agttgctcac ctggttctag cttcaaataa gcggcgatca attcttcctg agatagctta
    11641 tctctgtttg gtatccatgt tttgatatca gaagtatgtg tcagtaaatg acgcaaagtg
    11701 attgtctgat tttcaaattc aggaagatat ttatgaagcg gttgatcgag ttctatcttt
    11761 ccatcttcta atagacgtaa aactaatgaa gtggttgcaa cgactttcgt cagtgaggct
    11821 gcatcaaaca acagatcttc tgtcatcagc tgcttttcag gaacgacttg ggcaaagccg
    11881 gctgtaaatg tttctacttg attttctttt atgaaggcga ctgctacacc tggataaaat
    11941 ccttcttcca tctttttcaa aataagctgt ttcgtgtgtg gatacatctt tttctcctgc
    12001 ttttctatca gattcttaca gtccaagctt aaagcttaat ctatgaggaa tcaacctttt
    12061 ttacacaaaa aaactgcgcg aaaaaccgcg cagtttcccc cggtatacta ttttctcttt
    12121 ctatctctag aataagcgat agtatgaccg ttcataaaat gacgagacat gcgacaatcg
    12181 ttaagaaaga aaaagttaag cgtcgcatcg aatgtcagtt ttattctttt cttttggctc
    12241 actgacgaac caccattcga ttcctatagc atcatagatt gtgagtactg tgtgcttttt
    12301 gtctaagaag aaatcaactg atagttgctg caagtgagca atcagtcctt ccatgctttc
    12361 agtatcgatc atgaatttca gaaaatcaag tccaatcact tcatctgtag gcaaatcaat
    12421 tgcgcctccg ctagcttctg tcaaaccaac atggaaatct ccttcattca atacatggat
    12481 gccctcttct tcattatgta ccttcaaacc aagtacatga ttaagaaatt ggcgttcttt
    12541 tgttaagtta gtaacattta aatggatttt ttcaaaataa gaacctgttg atagtttact
    12601 gaatttttct tcagattttc ttaataatgc ttcttgatcc attgtttctg gaatttctct
    12661 ttggtcgttc ttattctctt catggtaaat ttccaattca ttctcttctg ggtctacgag
    12721 caaaatcccc atacgtccat gatcttctaa agcttctttg attggataat tcattttacg
    12781 gatacgacaa aggatatctg ccatttcttc tgtgctaggg ataatcaatg aaaaacggtt
    12841 cagctttttg atcaccccca tgtgatcatt cgctctagga ctttcttcca gcagcaacat
    12901 ttcacttcca gcttcttctg ttcctaaaat ggctaattcg ttttcttctc ttttcaaatc
    12961 aaagccaaca acgttcttgt aaaaatcaat cattttatct cggtctttca cccgaatggc
    13021 tacacttttt aaataggtgg cagcccccaa ttgaaaatct ttcatatttc ttgctcccct
    13081 ctacgtcaag ctttatttta acgatggatt cttcttcttt caagcaataa ctttgatttt
    13141 gttaaaaaaa atgtgagaaa attcgcattt tgcttgtttt ttgtaaatta atgagtaaaa
    13201 actcttataa aaagaggttg tgaaaaagct gttctgcatc aagtaataag aacagccaaa
    13261 caaaaatagc tcctcctatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt
    13321 gaacaccgtt taaggaagag gttgtaaaat cagcggtttg acctgagtat tagagataca
    13381 aaacggaaaa atagcttctc atattttgcc gttttttgag ctaataccga aggacagctg
    13441 attgaacacc gtttattcgg aaaaagggac tgtgacacga cttttgtcac agcccctaaa
    13501 aaaaataata aatttgtact attctatgaa gcttcttctt cagaaaactg actgttataa
    13561 agatctgcat agaatccgtc tttttccatc aatgtatcgt ggtttcctgt ttctacgatc
    13621 gaaccatgat tcatcacaat aatgttgtct gcatcgcgta ttgtagacaa gcggtgagca
    13681 acaacgaaac ttgtccggtt ttccaatagg cggttcatcg ctttttgaat cagtatttct
    13741 gttcgtgtat ctacactaga tgttgcttca tcaaggatca ggacatctgg atttgctaag
    13801 aatgcccgcg caattgtgat tagctgacgc tgcccttgcg aaatattgct tgcatcctcg
    13861 tttaatacag tgttatatcc ttctggcaat ttacgaacaa aatcgtctac gtgagcagct
    13921 ttagcagcct caatgatttg ttcgtctgtt gcatcgtcgt tcccatattt gatattgtca
    13981 tagatacttc ctgtaaacag ccaagtatct tgcagtacca ttgaaaactg tgcgcgcaat
    14041 tcatcacgtg aaagatcacg ggtatctaca ccatcgtaac gaatactacc tccagaaaca
    14101 tcgtagaacc gttcaagaag gttgatcagt gtcgtcttcc ctgcaccagt cggacctaca
    14161 atagcgacca tctcaccagg tttgacatcc aagttaaaat cagtcattag caagttatct
    14221 ggtgtgtagc cgaattgaac attttcaaaa cgaactttgt atggtgtatc tttttcttct
    14281 ggaaggccag atttttcatt ggacatctct tcttcatcca acacttcaaa tacccgttcg
    14341 gctgaagcaa ctgttgattg gatagtgttc atcaaacttg caagttgagt gattggttga
    14401 gagaactgtg ttgtatattg caagaacgct tgaacatccc ctaatgtcat gttcccattt
    14461 gcaactttta ctccgcctag tacagcgacg aacacatagc ctagattttt aacgaaattc
    14521 atcaacggca tgatgattgc cgaaataaat tgagctttcc aaccagcggc atacagattc
    14581 tgattttctt tttcaaatac ttcctggtct ttttcagtat gattgaatgt tttaacaatg
    14641 gtatgaccac catatgtttc ttctacttgg ttattcaata aacctaaact tttttgctga
    14701 tctgcgaaaa acacttgtga tttcggcgct acgatcatca cgatgatcaa gcttaaaggg
    14761 atcatcaata acgcgactaa cgtcaattgc cagctgattg aaagcatcat cacgatgacc
    14821 ccaatcaatg tgactgcact agttacaaat tgagtcaaat tttgctgcag cgtgcttgcg
    14881 atgttatcca tatcattgat tgctcgagac atgatatccc catttgtatg tgtatcataa
    14941 taggaaatcg gtacacgatt cattttggct tccaaatcat gacgtaattc ataaactgtt
    15001 cgttgggaaa cacgtgtcat aataaattgc tgtaaaaagt tgaatacagc agaaatcaaa
    15061 tacatcacga ttactgtcgc aatgatctgc gcgattttat caaagtctat cggaaatttt
    15121 gttacttgtt ggccttgttg catctgttga gcgccagcca ttacaccttt gaatatttct
    15181 gtggttgctt tccccaagac ctttggtgtt tggatttgga agatcacggc tgctattgct
    15241 agtacaaata cagcgatgat cgcaaccata cgtttagaca tatagccaaa caatcggcgt
    15301 accgttcccc agaaattttt aggcttttca ccttttacac cgagatttct gccaggtcca
    15361 ccatgtcctg gaccgcctgc tggtccatga gagggacgtg tgtttgtttt actcatgcta
    15421 agtcctcctc tcttagttgt gaatgaacga tttcttgata agtttcgttt gttgctaata
    15481 gttcttcatg tgttcctttg ccaaccagtt ttccttcatc tagcacaagg atcatatctg
    15541 catccattac tgtacttact cgttgagcaa ctaaaacggt gatactttct ttcatattcg
    15601 ttttcaatgc ttggcgaaga ttggcatccg ttttgaaatc caacgctgag aaggaatcat
    15661 caaacacata gatatctgct gttttgacta atgcccgtgc aattgccaga cgctgtcttt
    15721 gtccaccaga gaagtttcct cctccttgtt ccacatggct atctaagcca tcttccatct
    15781 ctgaaacaaa atctttagct tgtgcgattt ctaatgcttt ccaaatcatt tcatctgttg
    15841 catccggctt accatactgc atattgtctc ggatcgttcc agagaacaga actgcttttt
    15901 gaggaacaaa gcccattaat tctcgcaaat tatattgagt catgtcacta atgtccgttc
    15961 catttatttg gatcgaacca gattcgatgt catataatcg agggatcaaa ttgactaagg
    16021 tcgttttacc tgaaccagtc ccccctatga ttgcgacgat ctcgcccgac ttggcttgga
    16081 aatcaatatc ttccaatgca agtttttctg ctccatgata tcgataattg acatgattga
    16141 aggccaatgt tgcgtttttt ccagcaaaag aaactgtttt aggattttca gggtcatgga
    16201 taccgatttt ttcatccaac acttcattaa tacggtcagc tgaagcttgt gcacgaggca
    16261 ccatgataaa aatagcggaa agcatcataa agctgatcaa gatctgcatc gcataagtca
    16321 taaatgcaat cagattaccg acttccaaag tcatatctgc tatgtagtgt ccaccaaacc
    16381 aagtgattgc tacgtttgta ccgctcatga tcaatgtcat cattggcatc atcaatgcga
    16441 cgatcgtatt aactttgatt gccgtatttg tataatcttt attcgctaaa tcaaaacgat
    16501 tttcttcaaa gcgtgtctta tcgaatgctc gaatcactct gacccctgtc aatccttctc
    16561 gaaataccag gtttaaacgg tcagttttct tttgcatact tttgaataat ggaacagcaa
    16621 agtacatgat tccgccgatc aaaataatca ttactgggat gacaactaag aagatttttg
    16681 tcaactgatg atctttttga taagcaagta tgctcgcgcc aatcaacgtg attggtgcat
    16741 tgatcatcat acgtaaaaac atttgtgtaa ccatttggat ttgagtcaca tcattggtcg
    16801 ttcttgtgat caaagaggcc gttccatatt tatcaaacgc atcttttgtc agactctctg
    16861 attttttata gacatccgta cgcaattgct tacccagttt ctgtgattca cgagtagcaa
    16921 aaaatgtatt tgcgatagca gctaagatac tgatcaaaga aaagccgatc atcacaaaac
    16981 ctgtatgcca aatataatcg acatctcctt gcgctacccc tttatcaata atattggagg
    17041 taagtgtcgg caaatataaa tctgccagta cttggatcac catgaaaact gcagctgcaa
    17101 ttgctgaagt caaggagatc cgttttacga tttttagcat ctagttccct tctttctatt
    17161 tatatactcc gtttttcagc caagaaagtt tcaaattgaa ttcactgatt gcttgctcta
    17221 ctgagtaatc ttcagatgtt gaaaataagc gatacgaatg cgcaattgtt gtaaatacag
    17281 tattcatcac taattggatc agtaattcca attcatgatc attttttact ttcagcttcg
    17341 tccgatcgat caattcaagc aactcttttg catcttgttt gtgttcttta tgtggatcgt
    17401 gaggcgtttt ttggcgactc gtatgaggag cgacttttct tgaggcacgg taatccatgt
    17461 tcataaacaa gttacgataa aaagaagcat tctcgccttc caaaatctca taaatcattt
    17521 tagaaaaata ggattcgaat cctttgaata gatcaccatc ggattctttc attagttgta
    17581 acatatcttt agagctgtca cgtctcattg tctcaaagta ataaaaataa agatcctctt
    17641 tatcttcaaa atactgatag aaactgcctc tagggatatc cgctaatttg acgatattag
    17701 caatcgaagc ttcttttaag ggtactctag aaaactcgat tctcgcagcc tcgattagtc
    17761 ttttttgttt ttctttaggc aaatggaaaa atgtctgtgt cggcatatgg atcctccttt
    17821 ctttcaagat gacacattgt catatgacgc tgtgtcatta taacacctcc tacctgtttt
    17881 gcaagacatt tgcatagttt ttttcattat catccgatac attaaaaaag tgataagagg
    17941 aatccttcca tttgaattaa tgagagttgc ccattaaaat agccacatac taaacaaagg
    18001 atgagataaa tggcccaatc aattagtgtc caagacttca ataaattgcc tctcgatgct
    18061 gacagcatcg ttttagatac aagaaatacc ttagattaca caaaaaacca cttaagccac
    18121 tcgctttcta tccccgctgc tatcttgcca agacaattaa aaaatctaga tccaaagaaa
    18181 ctttattata ttctaagcta tacaggcagg cgatcagaag cattagccga tcaacttact
    18241 gctaatggtt ttcgtgctgt ttatatcatt ggtgggatgg aagctcttca gtcgagtgtt
    18301 gcttgaatat tgcttgttag attcaaaaga ggctgtgaca aaaaatcgtg tcacagcctc
    18361 ttttataccc tgtctttttt tagttattta ttcttcaacc ttatcaatat gacttgatag
    18421 ccttttcttt ttctcatagt tataacaatc atagcaataa aaggagaagg taagcatatg
    18481 gcatctgttt tagtagtcaa aggtcatccc ctaactgcag aagaatcgcg cacagttaag
    18541 gcattgacgt cttttttaac aagttataaa gaaaatcatc cagatgatga agtgactgtt
    18601 ttggaacttt atcgcgatga tattccggaa atcgatgaag aattattatc aggttgggaa
    18661 gcattacgtg ctggtgcaga attcacttcc ttatcagaaa gtcaacaaca aaaaattgct
    18721 cgtttcaatg aattgactga tcagttcctt gctgctgata aaatcgttat cgcaaacgcc
    18781 ttatggaatc taaatatccc aacaaaatta aaagcttggt tcgataccgt caatgtagct
    18841 ggaaaaacgt tccgctatac agaaaacggg ccagaaggat tagtgacagg taaaaaagca
    18901 ctgcacatcc aatctaatgg cggtgtatac aacggacaag actttgcctc tcaatatgta
    18961 aaaggtattt taaactttgt aggcatcgat caagtagatc agctattcat cgaaggaatc
    19021 gattatgacc ctgaccgtgc agatgagttg atgcaaaatg cattagataa agctgctgct
    19081 ttaggtaaat cattttaaaa attgagataa atgcatacaa aagagacaga ggaaaaaatc
    19141 ttcaaagcag catttgtacc ttggatattt tcccatctgt ctctttttgt tattctttct
    19201 gctttttcaa cggattttct tcataaagag aagagattgt tgtgtaccat tcaaaaacat
    19261 gagtaatcac tactttagct gctgcataaa caggaatccc caagatcaca ccgatgattc
    19321 cgaacaattt cccagaagtc agcagaacta ataaaatcgt tactggatgg attgctaact
    19381 gattccctag tactaatgga gagatcaagc gtccttctat tgtctgctct actgcaaaaa
    19441 cgatcagtac tttgatgatc atgaatggac ctgctacaat ggctaaaaag atagccggaa
    19501 tcatcgctaa aaatgatcct aagtaaggga tcaggttcaa aaaacccgca ataatcccca
    19561 acgtgacagc atactctaaa ccaatgaccg caaaaccaat cataaacatg attgccacag
    19621 caaaagccac agttagttgt cctcttatat aagaagaaac ttgactattc atttcactca
    19681 gcacattcaa cgtcggtttt tgcatgcgtg tcggtaaaaa tttgacaaaa taaggtgcta
    19741 attgtttacc gtctttcaat agataaaata agataaacgg catcgtcagc agagccacta
    19801 aaatagtcgc taccgcgccg acaaaacttc caagactctg aactgtactc ttagatatat
    19861 cttggatcat atcgcctgcc gaattcatga acttatcacc tgctgtttcc aactgttcgc
    19921 gaaattggtt gaacagcggg tcccgcaaaa tctcggttaa tttattatca atcgtatcta
    19981 catattgagg gaagttactg atgaagctca ctgtttgttc ctgaatctta gggatgatca
    20041 ctacgctccc ccatgcgatt agtgcaacaa ccaaaacaaa cagcccaatg atactgtaaa
    20101 gccttggtac ttttcgcttt tcaaagtaat caactaccgg attcatcaag taatacaaga
    20161 ttcctgctaa aatgataggc agacctacga ttgctaaaaa ttgccagatt ggtgcaaaca
    20221 aatatgaaac ttttgtaaat aaaaagacga tcagcaatat taacaataca acaagtaagg
    20281 ctgtgactac ttgattattt aaaaaccagc gccaaaacca cgataccggt ctgttcttct
    20341 tattttttcc ttccataatt cctcctgtta gttataaaaa atttggcttc ccaaaccaat
    20401 agcaggcagt ctatgcggat gaagatagat tccatttgca atttgtcctt cttctggaca
    20461 agcatcaaaa atcaatgata cgtgagctac tgtctctaaa ttcccattgg ccatcggacc
    20521 gacataatcg atcgtatata cctgatcatc aaaagcaatt gttccgcctg ctttcaaaga
    20581 aaaaggttcg tcttttgata cttcttgaat caccgaatat tttcgtaagg cagctgttgc
    20641 tgttttatcg aacaaaatca gcatgggttc ttccttgttt attgcttctg tcccaatttc
    20701 tgtaatcttt gcctgaatca ttgcactctc tcctttaatt gctttcaaaa acagtataac
    20761 acattctcat tctttagccg tgtggaataa ttatgtgata gaataaaaac gaaaactatt
    20821 tcattacaat taggaggaat cagctcatat gatcgaaaag ataattttag gaacctatac
    20881 gcgtcgcgaa agtaaaggaa tctatacgat cgaccttgat acagaaaaag aagaattagc
    20941 caacctttca ttagctgttg aagaaaatag ccctacctat gtagcaaaaa gtaaagctgg
    21001 caatctttac acagttacta gtgtggatgg attaggtggt gccggtgcgt acgataagga
    21061 ttttcatttt ttgaatgcgg taacagagtc tggcgctcct ttgtgttatg tagcggttga
    21121 tgaagcacgt caattactat acggggcgaa ctatcataaa ggtgaagtaa atgtctacca
    21181 tatattagga gatggcgaac tgaaagcagc agattcaata ttccatcaag aagcgacagg
    21241 tcctcataaa aaccaagatc atgcgcatgt ccactatacg gatctgactc ctgaccaacg
    21301 attagtcgta tgtgatcttg gtacagatcg tgtgtatact tatgatgtgt ccgaaaacgg
    21361 caaattgaat cttgtaacta ccttcactgc agagccagga acaggccctc gccatcttgt
    21421 ctttcatcca aatggacaat tcgcttattt atttggtgaa ttagacagca cagtccgcgt
    21481 gctttcttat aataaagaaa caggtgcttt tgaagaatta cagaaaatat cgaccttgcc
    21541 agaagaattc gacggtgaaa atggaggggc agctatccgc atttcaaatg atggcaagtt
    21601 cctttatgct tctaaccgag gacataactc gattgctgta ttcgcagttt ttgaagatgg
    21661 ttcccacatt caaaacaagc aaatcatctc aacagaaggg gattttcccc gcgatttcgc
    21721 actagatcca agcaataatt ttgtcgtgtg tgccaatcag aacaccgata atctgacatt
    21781 atatcgccgt catcctgaaa caggattact ggaattactg aaaaaagatg tctatgtacc
    21841 agaatgtgtc tgtgtttatt ttgatcgtta gtaagaaaag gagaatgaaa tgaatcagca
    21901 agacgtatta gacagactac gggaagaact aaatattcct ttctttgatg ggaaaatcga
    21961 agataaagag tatagcgaag aggaatatca gaaactcaaa gccgatttac agaactactt
    22021 tgaacaatat gtacagaatg tagaaaacta atcagctttc catactatct gaaaagaaca
    22081 tcaggacaag acttttccgt ctatcctgat gttcttttct acaaataaaa aaccgccgag
    22141 atggttagac tcgacggaaa aggagtattt actcgatttt gagtaagtac aaggttgttg
    22201 ttggttatag gtatgtcatt atcataaagg attttgatca ctctatcata aaggtaatat
    22261 tacatttata ttagataact ctagttttta ttgttatcaa agatttcttt tattttttca
    22321 acaacaaatt agtcattttt tggatggcag ctgtcaattg gtctgtcgct tggtcttgtt
    22381 ttgcttcaga tgtattgaat agatcttcat ctacccctga ttcaagatcg atactttgga
    22441 tcatttcttc acagcctgct acatatgaaa gataagcttt ttcaaaattt ttatgaatgc
    22501 ccattgcttt tgctggtgga cgcaaagtac tgactttctt aagcattgcc cgatattttt
    22561 ttgttccttc ttcaaataac aggatgatct cttcaagagt cgatttgtcc agttcacttg
    22621 tttttccttc atcaattgcg tttctgatca cttcatattt cggatgcatt ccttctccga
    22681 ttgcttctgt ttccttgacg atttgatcga tggactgcac ataatagcca atattcggta
    22741 acatgttcgt tttcctactt tctccattgt tgttattatt tattctactt gtttttatcc
    22801 tcaaatgaca agtacttgaa aaaatttaat ggtttctata cagctcgcca ttcttattcg
    22861 tgctcaaaga aaccatctgc taaactatat tgtttccctg cgacaatgtc atattggatc
    22921 aattgatatt cgtcgtaaat ctcttgttgc gagcgattga attgcgcttc tttataccac
    22981 tgtccgtttt gatagtacag ttctcgttca aacgccggaa gttcctgttc taatgataaa
    23041 agcagctgat aaaagccatt tgttgtctga ttcgtctgtt ccattagatt cgctgcaaaa
    23101 taaaacggac tggtgattgc atcgtgagtc gtttgctttt ctaattctcc tttactgtcg
    23161 aacataagaa attgtgtttc atgcaatgtt tgtttgtcat tttttgcttg gatcgtatct
    23221 gaatagatcc ctggaagatg atccccccaa aaaacaacta aagtccttct agaaagattc
    23281 ttcaattctt ccgtgaactg cttcagtgcc gtgctgctat aggaaatatc ttgcagataa
    23341 ttctctaagt caagtgtccc acttccatct gaagtttttg caggatagtc tagattatca
    23401 tactttccat cgtagggcat atgtgtctgc atcgtgacta aatgaatgaa ctgaggtgtt
    23461 ttttcttcct tgagaagtgt cattacttct ttgtaagctg actcatcaga aatatatgga
    23521 ttattctcta tcgtatccgt ataggtcatc gtacgctcgc tgatgaactg gtcaaatcct
    23581 aatgtctgat aaacatcctc tcttttatac atcgaagtat tgtatggatg gatcgctgtc
    23641 gtctggtatg attgtgcgtt taacgctgaa acgatagaag gaagctgatc catttttggt
    23701 acaagcatcg tgtaaggggt ggtgagttgc gcattaaata acgccataga aaaaccagtc
    23761 aatgcttcaa attcgatatt tgctgttcca ccgccatagt tttgagaaag catattgcca
    23821 ctatatgttt gatcagctac ttcataataa tctgccaatg gctcaccaga tacttctacg
    23881 ccgtttaatc gactaggatc tgagaaactt tcactcatca cgaacacgat gttcggcgac
    23941 tcttcctcta cagttttatt tttttcatcg gctagcttct ggtatttttc agtgatttct
    24001 ttgatctttt ctttcgaata tccttcaggt tcatccattg gttctacctt cagattgtac
    24061 aaaaacccac cgataaaacc agtgttatag tagttcattt tctggctgta aggtatccat
    24121 agtgccgttt tattatatgc ttttcgcaac aaattatcgg gattgttgaa attactgata
    24181 taccctagca gtccaattgc cacaagcagt gtcgttaatc ggatcaattg gaatcgcctt
    24241 ccttttttca ggcttcgaaa catgtaccag caaaacaatc ctatcacggt tcctgcggca
    24301 gctaagatca ctgtaaacag catagtgccg gtcatatctt tcagcaatcc gatttcagtg
    24361 atcatcttca aatcgtctgg ataaatcggt tcttgccgat aactcatttt catataattt
    24421 gcaaaaccta atacaccgat ggctacgaca tacgtaaggg aacctaaagg gacagatcct
    24481 gctagtgcaa tcagaaaaat aagaataatc agcaagacga gacaggctaa gaagaatttt
    24541 tccgtatgcc aagaaaaagc aaatttcaac gcaaggtcta cggatagatc attttgacac
    24601 cactgcaaaa ataaattact aaaaacaaca agaaaaaaga tactgatgat tgctgctgtt
    24661 ccttttaaaa tctttttctg tttggtcgaa agtctgctta tatatctttt caaatgcttg
    24721 ctccttcatt tctatcaata aaagaatgtt gcttctctta ttttagttgt acgaaattcg
    24781 aaagtaaaga acggaaagaa gaattaatga aatccaaata ttctctttct ttatggcctt
    24841 tctggtttct ttaataagcg gacctttcgt actccagaaa ttttttctag ctgttgtttc
    24901 acttgattga gctgttcaaa atcagtactg tctatgtcaa tcaatatgta gacatgctca
    24961 ttcttatggt tacgcgatat atctgcgatc gctagctcgt tctcattcag cagagaaaat
    25021 atctctgcta atatattggt tttcttttga taaaatatag tgaatcgaaa cggcgcttgg
    25081 aaaaacattc gtgcggatgg ataattgacc gcttctctaa ccgtaccgaa caaaagataa
    25141 tcttttaagg cttcaactgc caaacgtccg ctgtctgcca aagcttcctg agtcgatccc
    25201 cctagatgag gcaacataag tatacggtca ttttctaaga actcttcttc tggaaaatca
    25261 gtcaagtatt tagccagatg ttgtttttcc aatgcttgca taacagcata tttatcaaca
    25321 atttcacttc ttcctacatt gataagtaca gcatcttttt tcattttttc tatattcgcc
    25381 tgatcgatca atccttttgt atcttctgtc aacggcagca tgactacgat gtaatccgag
    25441 gtagaaagaa ctgtttctaa atctgcttgc cgaacataat ccaactgggc atcgcggata
    25501 gaataaccca agacatccat ccctaagcta tagcaactta gcgctacttt tgtgcctatc
    25561 gctccaagac caagaagacc gatggttttg ccttgcaatt ctcgtcccac ataagcactt
    25621 cttttgtttt ctgcctgttc taaaatatta ggacctgtta gtgtttgtac catacgactt
    25681 gcttcgatta tcggacggga agataagagc aaacagcaca aaacaagctc ttttactgca
    25741 ttagcattta cacctggtgt attcatgacg atcgtcccgt tttcagaagc tttttcaacg
    25801 ttgatcgtgt tcaccccaac accagctctt gaaattgcta gcaaatctgg tgtaagccat
    25861 tcatctttca ctttcgtact acgaataact attccttcgg ctggcaaatc atctattatt
    25921 ttgaaatatt ccccttgtaa cttttctatc tcttgttctg gaatagcatc aatcaaatga
    25981 acgtgataca taaaaccccc tccttactaa caagtatctc gaaatttctg caattagaca
    26041 actgtttacc atttgacttc ctagaagcaa aaagagcagt tagagcagag taggctgagt
    26101 gataaaaata aggctatgac aagttactgc cataagcctt attttcgaga gcaaaatatt
    26161 gagggtgaga tatcttgata aatggtgttc cgttagctga cattgataat aagactaaat
    26221 ttcaaaaaag ttgaaaagca aatttcgtca atctcttctt atttcttaaa actaagtggc
    26281 tttttcacaa cctcttttta tctaataacc actgtttcca actcaactaa ttttgcccac
    26341 gtttcaattt gatctgttgt tagatttagc gagacaacgg tatgatgacc gccacctgcc
    26401 tgaatccatg aatggacacc ttcatggaaa ttcggtttag ttttccataa cacacgtgca
    26461 acaggtaaat taggtgcttc agtcgttggt tcaaaggctt ctacttcatt gatcagcagt
    26521 ttataatgcg taccgaaatc tgccatcgat acgactaccc cttctcctgc tttcccatca
    26581 aatacaagac gcgccggatc ttctctatct cccattgata aaggagaaac aacgattctt
    26641 ggtttcgtcg cagcaagcgt cggatctact tccatcatat gggattccaa gattgcttct
    26701 tggccactag ctagctcgta agtataatct tccatgaatc ctgtatcttt ccctcgagcc
    26761 atgattttca gcaaccgatc aatcgctgca gttttccagt cgccttcccc agcaaaaccg
    26821 tatccttctg ccatcaaacg ttgtacagct agtccaggta gttgtttcat tccatacagg
    26881 tcttcaaaat tagtcgtaaa agcgttatag cctccttctt ctaagaaacg gcgaataccg
    26941 atttcctgct gagcttgtac ttttacatgt tcctcccatg tcttttcttc ataatcacca
    27001 tagtcgaagg tatacaattc cttgtactct tcaaataatt gatcgacttc ttcgtctgat
    27061 acgcgatcga ttacttgtac aagatctccg ataccaaaat aatcaacagt ccaaccaaat
    27121 tgaatctgtg cttccacttt gtctccttct gtgacaccca cgtttctcat gttgtcacca
    27181 aaacgagcca cttttattcc aaaactttca ttataggcta ctgcaacatc catccaatca
    27241 gcaatctctt tttggatctc aggtcgtttc cagtagccaa cgacgatttt attttgttta
    27301 tttaatcgag cattgatgaa cccatattcg cggtcaccat gagcggattg atttaaattc
    27361 atgaaatcca tatcgatcgt tttccaagga atgctttcgt tatattgcgt agctaaatga
    27421 agcaatggtt tttgtagaag ctttgtcccg cggatccaca tttttgctgg tgagaaagta
    27481 tgcatccaag tgatcacacc tgctacttct tctcgataat tgacctcttt catgattttt
    27541 gtgatcgtat cagcagtaac tgctaattct tgaaggcgga ttggataagg caattggcca
    27601 ttttcattca gttcatcgac catttcttgt gcatgtttct ttacttctcg cagtgcttct
    27661 tccccatata aatgctgtga tcctacaaca aaccaaaatt ctttttctcc aatattcaac
    27721 ataattagcc actcctttta tttgttttgt ccgtaatacg cattttttcc gtgttttcgt
    27781 aaataatgtt tatctaaaat ccgttgaggc agctcttctg aaaatgaatt gagctgtctg
    27841 gtaaataaat tcatcttgca tacttcatct agtacgactg cgttcattac cgcactttgc
    27901 gcatctttcc cccaagtaaa tggaccgtgt ccgtgtaaaa gtacccctgg aattgctaag
    27961 ggatcaattt tgcgttcttt gaatgtttct atgataactt ttcctgtttc atattcgtac
    28021 ccgctgtcga tttcctgttg tgtcaaaaat cgagcacaag gtaccgatcc ataaaaagta
    28081 tctgcatgcg tcgtccccat cgcaggaaca tccaatcctg cttgtgccca gatcgtcgcc
    28141 catgtcgagt gagtatggac gattcctccg atttgaggaa atgaacgata taaaacagca
    28201 tgagtaggcg tatccgaaga gggattgagt ttaccttcaa tgatatgatt gtccaagtcc
    28261 acaacgacca tgtcgtctgc tgtcatctct tcataactga ctccgctagg tttgataacg
    28321 aaatatcgtg tttcaggatc aaaagcgctc acatttcccc atgtatattt gacgagtccg
    28381 tgtttaggca agtcaagatt tgcttggtat acttcttctt tcagttgttc cagcattttc
    28441 tatcactcct ttttcttgca attgatgaat cgctgttttt tcaatgtcga tcccctcacg
    28501 gtaacgtctc ataaatatct gatagcctgc tatttcttct gctgaaggtg tgatggtgat
    28561 tccttggtct tgtccaaaca cgtcttctgc caagaagttt tctaaagact tcccatgtcg
    28621 atccatcata taggctgcta ataacgcgat tccccacgct ccaccttctc cggctgtgtc
    28681 catcactgta actggtgcct ccatagcaga tgctagaatc cgctgtccaa cttctggtgt
    28741 tttaaaaatc cccccatgcc ccactaaacg atcaatttgg acattctcag atttcaatat
    28801 atccattccg attctcattg caccaaaagc actggctaaa tggatccgca tgaaattcgc
    28861 taaatcaaaa cggctacctg gtgtacgtac aaataatggc cttccttcat ttactcctgt
    28921 aatgttttca ccggaaaaat aaccataaga aagtaatccc ccgcaatccg tatcgccttc
    28981 tagcgctttg ttgaataata ccgtgaataa tttatctctg cttatctcca tacccaaaga
    29041 attggtaaat tcttcaaaaa tactgaccca agcattgata tccgaagagc agttattcgt
    29101 atgaaccatg cctactagtt ctcctgaagg tgtagtgacc atgtcgattt ccggatggac
    29161 attttctagt tctttttcta atacgatcat agcgaaggcg gaagtaccag ccgaaatgtt
    29221 ccctgtccgt ttggctacac tgttcgtagc gaccataccg gttcctgcat ccccttctgg
    29281 cggacaaacc gggattcctg gtctcaaatt tcccgtaggg tcgatcagat aagcccctga
    29341 ttcgcttaac ttgccagctt cttctcctgc tactagaact ttaggcaaaa tgtgatccaa
    29401 gtcaatcgct acgtgcgctt tttcgattaa ctgaccaaaa atatttttca tttcttgatg
    29461 gtaggttttc gttgaaggat cgataggaaa catgcctgat gcatctccta cgcctagtac
    29521 tttctcacca gtaagctgcc aatggacata acctgcaagc gttgtcaaaa aattaatttc
    29581 tttcacatgt tcttctttat tcaaaataga ttgatacaaa tgagcgatac tccatctctg
    29641 cggaatattg tattgaaatt tttccgttaa tattcgttct gcttcttcag tgattgtatt
    29701 tcgccaagta cgaaatggta ctagtaactg ttccttgcta tcaaatgcta gataaccgtg
    29761 catcattgca ctgaacccaa tcgacccgat tgtcgtaaga ttctcgccat agatttcgaa
    29821 tacttctgct gccatttttc ggtaactgac gcgcagtcct ttccaaatat cttctaatga
    29881 ataggtccag ataccatttt ctagtttgtt ttcccactca taactaccag cggcaatagg
    29941 agtaaaagat gaatcaatca atacagcttt gatcctagtt gatccaaact caatccctaa
    30001 agatgtacga ccatctatga catctaacat actttgcgca tttacttcct tcataatgat
    30061 ttaactccct ccttctcgtc aaacctgatc aaaccgtctt tttagtagcg cttacaatga
    30121 ttattgtata cttttgtacg tacatgtcaa gcggttattt acaagaatac aataaaaaac
    30181 acttgttttg tacgtacata agttataaaa tgaaattgct tacaaaatct gatcaaccgc
    30241 cttctcgaaa ttctagaagg agagaatact tatgaaaaaa gaaaaaaaag tttcctcatc
    30301 gtttatttat ttttttggcg catttggcgg cattctattt ggatatgata ttggtgttat
    30361 gaccggcgcc ttaccttttt tacagcatga ttggggtttg gcaggtaaag cttctttgat
    30421 aggatggatc acgtcttcgg tcatgttagg tgcgatcctt ggcggctctt tatctggtct
    30481 tctttctgat aagcttggac gacgaaaaat gattcttctt tctgcactta tttttatggc
    30541 tggttctgtc ctttctgcat ctgcccctca caatggttct tattttctga ttgcagcaag
    30601 gattcttcta ggtttagctg ttggcgctgc atctgcccta gttcctgctt atatgtctga
    30661 aatggctcct gctagactaa ggggaagatt gtcagggatc aatcaagtga tgattgcttc
    30721 tggcatgctc ctttcctatg ttgcagatta tttgctaaaa gtgttgcctg aaacgatggc
    30781 ttggcgcgtg atgttagggc tcgctgctgt ccccgcgctg attttatttt tcggtgtttt
    30841 agctctacca gagtcaccgc gttttcttat gcaatctggc cgcttggaag aggcaaaaag
    30901 agtattgaac tatattcgga caccaaaaga agctgaacag gaattcgaac aaatccagct
    30961 gaatgtcaaa caagaaaaga caaccggaac ttcttggcat actttgtttt tagaaaaata
    31021 tcgttcctta gtcattgccg gtattggtgt agcagtattc cagcaatttc aaggagctaa
    31081 tgctattttt tactatattc cgctgatcgt agaaaaagcg actgggcatg cagcgagcga
    31141 tgcattgatg tggccgatca tccaaggaat cattttagtt gctggttctt tgctcttttt
    31201 ggttattgca gacaagttca atcggcgaac tttattgaaa ataggcggta gcgtgatggg
    31261 actttcattc atcctaccag ctgttttagg aactgtactg gatgctcata caaattcgtt
    31321 gttgattctc ttgtttttat gtatttatgt cgcattttat tcttgcacat gggctccact
    31381 gacatgggta atcataggcg aaatattccc actagctgtc cgcgggcgag catctggcct
    31441 agcctcttct tttaattgga ttggttcttt cctagttggc ttgctttttc ctgtgatgac
    31501 tgcttctatg tctcaagaaa tcgttttcgg catttttggg atcatctgct ttttaggggt
    31561 tttatttatt caagagatcg taccagaaac tcgtgggaaa tctttagagg aaatcgaaca
    31621 aagtgcatct aaaaaaactt atcctaaacg tatttctatc catcattctt aaagaggttg
    31681 tgacagaagt ggtcagcttc aagaaataag aaggaaccgc ttagtttcca gtcacaagga
    31741 acaatttcaa aaaacagctt ctcatgtttg atgaatattg tgacttgtga ccagttggta
    31801 ggagaacacc gtttagtgga gaggtggtga aagaaacgtt cagccttgag cgatgacaaa
    31861 agctctcaca aacattgcga cgagtaaccg taggagcaat aagaggcggt aaaaacccgt
    31921 ctcttatttt tcgttttctt tcactgtttc ccgttcaact agtacaggtt catagagaat
    31981 atttggctgt gcctgtcctt tttcaatcgt tgtgacgatc caccgtgcgg catcttgtcc
    32041 caacttttct tttggatgcg tcaatgttgt caatttttta ttccctaaat ggcttaaaga
    32101 agaatcgtct gttcctacga tcgataggtc atctggaata tgatagccat tttctgtcaa
    32161 cttatctagc attaaactag ctatttgatc gttatagcaa atgatgccat ctaaagcttg
    32221 ttcttctttc aaccgttgta ctgttttttc agcgattgtt tctcgtgttt ccgtggtgaa
    32281 agtaatgata tcttccgtca aaaaacgcac attatactgt tcacacgcgc tgataaaacc
    32341 tttcatcctg tattttcctt gaagatcgtc gatttttgtg atgaacaaca gacgctgatg
    32401 tccttttttt atcaaatatt ctgctgccat aaaaccgact ttcacatcat ctaaacaaat
    32461 ataagaggca tttaactctt cataagcagc attgatcatc accatcggaa tgccttgttc
    32521 tctcaaagca acataaagtg ctaagtttgg gttatactga ttacttttcg taggctcaac
    32581 gatcaaacca tctaccccat aagcaatcat tttcttcaaa cactctttct ccaattcgtg
    32641 atcgttattc gtactggcta aaagcaatga atacccagat tctcgtaatt cattttcgat
    32701 tcctcggata atcgacggaa aaatataatc agagagatat gtggtgatga caccgatcgt
    32761 ttttgttctg ttcaatggtg ttttttcttt cttatagctg tcatctacat atgtgcccga
    32821 ccctttttct ttacgcagat agccctcatt taccaacaca gcgattgctt gccgaatcgt
    32881 atgtctgctg acgtcatata tttcttgcag cttgaattct gagggaatgg ttgtatttac
    32941 ttcgtattcc tcacgaagga tcttttgttt tatctcttct gcaatatctt gatattttgc
    33001 catttgcctt gccccttttt atttgtacgt acatatatca taacgagtct gcaaggttta
    33061 ttcaagcaaa aatcaaggtt tcttgtgctc ataaggcata cggaacaggt tcaccaaatt
    33121 ctggaaatcc ttgctcattc cacgtaaagc attgcgcgtt cgcgtgccga ttaggattgt
    33181 ctaacggatc ccctttttgg ttcttctctg gtcgagcatg atagatcaaa atatcaaagc
    33241 acccgtcttc tgaaaccgta aacgagttgt gaccaggacc aaattgctta tttttttcag
    33301 aagaaacgaa tactggctcc gataatttat gccatgattc ttttaataaa agattcttgt
    33361 gctcatcagc ccacagcagg cccatggcat agttttcatc tgttgcacta ccagaatacg
    33421 taatgaatat tttcccattg cgagtaagga ctgctggacc ttcatttact gaaaaacccc
    33481 gtttttccca ttcatactca ggtatagaaa ggaggatctg cttccccttt aacgtccaag
    33541 gattatccat ttcggataga taaagattag aattcccagt aatttcagga tcttgttggg
    33601 cccacacata atatagcttt ccttgagaag agaaagttgt tccgtctaaa ctgaaagttt
    33661 cccttttcgt ttttatttgt cccttttcta tccaatctgt attcattgga tctatatgct
    33721 gattttcaag tacgaacatg cggtgatgat gagattcatc acgtatagtt ttatgattgc
    33781 ttgcagcaaa gtaaatatac cattttccat tgatataatg gacttcaggt gcccagatca
    33841 attcactcat cggtccttct tcatgagcct gccatacaat caggcttgca gcatgttcta
    33901 attcattcag tgactttgcc cttcttaatt caatcacttg gtagcctgga accgatcctg
    33961 taaaataata ataaccatct gtatgtttat atacccaagg atctgcccgt tgccgaacga
    34021 taggattatt ataaagtgtc ttcatcataa caaatttcct cacattttct ttattctaat
    34081 tacattccaa gagtattttt ctatccctac atttaattcg tcatgttttt ctattgaaaa
    34141 atagtttatt tcaggtttta ttttttctgg ttcttcacgt gtatttttgt ccaacagatc
    34201 gctactggtt aatgtgtagt gctctccttg atctagcaaa ttgaaatcat gttcaatcgt
    34261 tatcttttgt tgttcatttg tgcgattgat caagaaaata gtcagctgat tgccgttatc
    34321 tacgatcaca ctgtccacat aaggaacatt ttttatcttt tcacaatcat aaacagggca
    34381 aatgatttgt tgtttcaata cttgaccttt agcatatttc gataagtgtt gcatcacata
    34441 ataagttgga ttcctccatg cttgtccttt agcatctgtc agtattaaag gtattgtgtt
    34501 tactaacaat gattgacaac taatctttac gcgatctgcg tgtcgtaaaa tagccagccc
    34561 taatgaacca aataataatg tatcttccat agtaaagtaa caccaatcga tgggactacc
    34621 gacttcaaaa tccctatgtc ttttttccgg ttgggcatgt acattccatt catcaaacgc
    34681 tagatacatt gttttctcac ttctcaaata tccttttaca taatcacaag tagcaacgat
    34741 ttcttcaatt tgacgatcga atcgataagc ttttgcaagg tatgtcgctg tatcatcacg
    34801 ctcattttta ggaggtttag tcaagtcttc ttcatcatac ttatcaatat agttatgcaa
    34861 agctaaataa tctacattct cataagcttc ttctaatgcg atacgatccc attcagggta
    34921 cgatggtaaa cgtggcgtag agcttccaac taagattgtt tcaattgaat catccactaa
    34981 cttcatgact tttgaggctt catttgctaa cctaccatat tcataagcac tcttagcccc
    35041 cacttgccat ggaccgtcta gctcattccc taaacaccat aatttaacgc caaaaggctt
    35101 tttttgccca ttttttattc ttaaatcact ccagaaggtt ccttttggaa aattacaata
    35161 ttctactaaa ttttttgctt catcaattcc ttttgttcct aaattcaccg tgaatatcgg
    35221 tttcgcttgt acatgactga tccatttaaa aaattcgtgg agtccaaaag aatttggttc
    35281 tatacctcgc catgctaaat caattttttt tggtcgttgt tctttcctac ctacagtatc
    35341 cacccaatca taaccagatg taaagtttcc tccaggatag cgaatgacac ctaattctaa
    35401 ttcctttaca taagagagga catcttgtct aaacccgtca ccatcagctg tttcatggcc
    35461 cggttcataa atccctgtat agacgacact tcccatatgc tcaacaaatg agccaaacag
    35521 ccgagaactg atctctccta ccgtaaaatc ctctctgatt tttactttca cttcttcgtc
    35581 caacagcttt ccctaccttt catttcctat agtcaagata atagatacaa gtttccatac
    35641 gccaccagca aagataaaaa taaaaggagg gaaaagaaat atgagcagta caaatcctaa
    35701 taacaatacg caacaaagaa tatttctcaa aaaatcaaaa aatactttcc atgcactttg
    35761 ttcaaaaatt tgtaaaaatg tgatatcttt gttttcagaa atcacatata tccaggataa
    35821 aagaaacatg gaataaataa gtaaaactac atatccacta attgaaagta cgattgtctg
    35881 ttcttctaat aattgattaa caagaaaata aataatgatc gttaacagac taaggaacaa
    35941 gttcttacca aaatgttgct ttattccttg aaagaaaaaa taaatgatat ttcctgatcc
    36001 ctcacctttc cattgcgcca ttacataaaa aacagcacat gtagcaggca cgatagtaat
    36061 aacaggcaaa cacacgatta cccagagaat acctaataag atgtaatcag aaaccacaat
    36121 caactgtcca taaaatttag tctcaactat tttcatcttc ctcactcttt cattccagac
    36181 atagctgttc cttgaacaaa atatttctgt gcaaaaatat aaaccactag taatggcagc
    36241 attgaaataa ccgttccagc cattacaggt ccataaaaag aaatgttctg ccctgtaaat
    36301 aaagctaacc ctgctggtaa agttctcatt tttgaactac ttgtcaatat caatggataa
    36361 agcaagtcat tccaactatt catcagcgtg aaaatcccta atgataaaag agtaggtttg
    36421 ctcaatggca acatgatttt caaaaaaata ttaaactcac ttaatccatc aatgcgtgct
    36481 gcctcttcca aagaatctgg taaagtaata aaaaaggaac gcatcatgaa gataccaaat
    36541 gcagttgtca tacgaggaat gatcaaacct gcataagtat caagcatgcc aagcaaattg
    36601 acttcaataa atagaggaat catgaagact tggaatggga tcatcattgt cagcaaaacg
    36661 aaatagaaca aaactgattt tcctttgaaa cgcatacgag caaatgcata ccccgcaaga
    36721 gaatcaaaga atacagaagt gatcactgtc cctccagcaa aaatcactgt atttttgatg
    36781 taatctaata aagggatcgt tgtccaaacc ttaatatagt tatctagggt atagctatct
    36841 gcaaatattc taggcggaaa tgagataatg tctttttcgt gtttaaaaga agaaataatc
    36901 agccacaaaa aaggaaacgc aataaccaac aatataacta gcatactaat cgttcgaatg
    36961 atttttttac cactaactgc ttttcgacta ttcacagtaa ctgtaatcga gctttctggt
    37021 gccatacagg tttccctcct atttcatttg tttttctttc cgtgacatgt acacattagt
    37081 ggagatagct atagttgcaa caatgataaa caaaaccgaa gacagtgctg atgcatagcc
    37141 caattcataa ggtgctgtaa atcctcgact gtaaatatat tgaacagctg tttctgtctt
    37201 aaattgtggg ccaccagcgg ttgcaacata tacttggtca aacacttgca tcgaaccaat
    37261 cagattcgtt aaaatacaaa aacctaagga gggaacgatc tgaggtagcg taatgttaaa
    37321 aaactgctgt cgatttgttg caccatccat ttgagcagat tcatacaaag agggagagat
    37381 tccctgaata gcagtcaaga gaatcgtcat agtaacacca aaattcttcc aaacagtcat
    37441 gatggctacc gtcggcatag caagggttgg atcgcgaaaa aattgaggga tcggcaagcc
    37501 gacttgtgaa aacaagtaag gaatcgcccc tacattcgga tcaagcaaca tagaaaaaat
    37561 aatcgcaatt gacgtcaatg aacaaataac tggaatgtaa aatgtcgaac gacaaaaacg
    37621 attgaatctg gtatttttag ataatgctac tgctgctaat aaaccaacga taatttgtgt
    37681 tggcgtttcg agtacggtga aatataaaga atgaatcaac gaattaactg ctcgttcatc
    37741 actaaatata cgtgtgaaat tttcaaaact tacaaaattt cttgatgtaa agaaaatatt
    37801 caaatctgta aaactaatag caaatgttcc taaaagcggt atgacagtaa ataataataa
    37861 aactaaaaaa gatggagtta aaaacaaata cgcagcacga tcaggacgat tccaatattt
    37921 ggtgaatccg ttcatatagt ccacctcatc tttcattttg ataaaatata caatcttggt
    37981 ttaaacgaat ctgaataaga tatccgaaca atcgctgtga tttttgcaat aacacctatc
    38041 tgcaaatact aggtcgatca ttcagatatc tgtatcttgc caacagtcta tcctgtataa
    38101 agttctgtac tatttttgca gtaattgatt gatggcatca tctgcatcgt tcatgagtgt
    38161 ctcaggatct tctcctgcta ataatttttc aatcagtgga ttgatgatat cgttattgat
    38221 cgcagagatt tgggtaaaac ctggtaaata tggctctgca tagtccattt gtttcgataa
    38281 ttcagataca ataggatcat tttttacttc ttcatctttt gctactgaat ccaagtaggc
    38341 aggaaaacca ttttcgatac tccatttttt accaatttca gttgtattcc aatatttcaa
    38401 aaactcatag acagcttctt ttttattatt atctgtactt gccggaatgg catatcctac
    38461 gccatctaaa atagctgttt ttttgtctga atcactccct tgtggtaagg tagtcacatt
    38521 ataatcaatc tcgtttgctt tcaatccgtt atttaaccat ggcccattga tttcaatggc
    38581 tagttgacca gcattcatta aattgtccat ttcggcgcca gaaatcgatt ctggaccagt
    38641 cttgtctaca tggatcattt tttggatcgc ttttaacgtt ttaaggttct caggagacgc
    38701 aaattcagac ttagtataat catcgttcac taatttaccg ccatttgcta acatccagtt
    38761 atataacggt gcagttccat ccttattgaa gacaaaacct gatacatttt tactactatc
    38821 agttaatttt ggtgccattt ctgtcaattc ttcccaagta gtaggtggtt gttcagtatc
    38881 taatccagct tgtttaaaca aatcattatt ccaatacata tacatgcctt gtacctgcat
    38941 ggggacaaaa tactgtttac cttctacttc acctaattca atagccgttt ctttaaaatc
    39001 agatttatct actccatcat attcccagaa atcgtctaat ggttgtaccg ctccgttttc
    39061 tacatactgt gcaaaatctg gataatttaa tgcaataaag tcaggtgctt ttttagaaga
    39121 aatcgctgta ggaagttttt cattcaaatt ggcccaagtc atgatatcca tttcaatttt
    39181 gatgttttta tcatttgttt tattaaattc attaacgatt tcttgcaata tttcaccatc
    39241 agatgcagta aacccattcc aaaaagtcaa agtcacttct ttatcagaat cagattctgc
    39301 tgaacttccg ccaccgcaag ctcctaaaat acctgttgca gctaccaaca aagagccaat
    39361 taaccatttt ttacctttca ttttttgttc ctccttcatc tgataaacga catctgacca
    39421 cattccaaga tagcggtttc aataaacaat tgattttccc atcttcagct tttacatttg
    39481 ttgaatgact tggttttact tgttcacttt taggaagatt agttgctttt gtatcaaatc
    39541 cactcatttc agaaaaatca atcactccct ctaatgataa ttcagacaag tgaacagtta
    39601 gattcatttc ttcttctcca cggttaactg caaatatgac aagttctttg tcatttatta
    39661 cggctatact ttctaaatac ggcacttctt caaaatcttc cgtcgcatag tttgctgaac
    39721 gaatttgtgg gcttaacacc tctccttgtc catacacagc agcttgcata aatggataat
    39781 agatcgtctg tctccaagct tctccgtttg tttctgtcct gattggtgca ataacgttta
    39841 ccaattgagc aagacaagca atctttactc tatctgcatg tttcaataaa gtgattaata
    39901 gacagcctaa tagtaatgca tcttcaaaat tatagttatc ttctagtaaa ggaggagcaa
    39961 tctgccaagg cgctgcttgc tcatcttgtt ccgctgaatg ataccaaata ttccattcat
    40021 caaaggacag gttgatctgc ttcttactat gtttcttcgc cttgactgta tcgcacatcg
    40081 ctgtcacttc agaaatgaac cgatccatgt ctaaggaacg tgcaagataa tgagaaagat
    40141 catttttttg gttgccataa tattgatgca gagacaaata atctatttga tcgtacgctt
    40201 gatcaagaac tgttaactcc caatcaccaa atgtaggcat ttgccgattc gaacttccgc
    40261 ataaaaccac ctcaattgaa tcatctacga gtttcatcgc cttagccgtt tcctctgcta
    40321 accgtccata ttcttccgct gttttatgtc caatctgcca aggaccgtcc atttcattcc
    40381 ctaaacacca tgtcttaatt ccaaaagggc tctcttgacc attcgaacgc cttaaatcac
    40441 tccaataagt tcctttagga aaattacaat attcgactaa attacgagct gcatctactc
    40501 cacgagtacc cagattgaca gccatattga tttcggcccc cacatgctga caccagttat
    40561 aaaattcatg aatcccaact tcattcgttt caattgtccg ccaagctaaa tccaaccttc
    40621 taggtctatt tgctatgggc ccaatactgt cttcccaacg aaatcctgac acaaaatttc
    40681 caccaggata tctaatcatg ggaatgttca attcttttac caagtgaatg acgtcttggc
    40741 gaaatccact tttattagct gatggatgat ctggctgata aatcccttca tatactgctc
    40801 gtcccatatg ttcaataaat gaactgtaca accgcggatc aactttgcct acacgcttga
    40861 cactactaat caacattgtg gcttccataa ctgacctcct ttaatttata catgaataat
    40921 acacctcgtt gaaagcgttg tcaataatat aaatgttaaa cttacttatt ttttatcgat
    40981 tttaattaac caaaatatta ataatgatta aaacaaataa tttaatataa cttttattaa
    41041 caaatatgtt aatatataag aaaaaccttt taagaggtga tatatatgca actacaaata
    41101 aacgaagaat ccttaccggt atatgaagca ttagctagta aaacaaggat caagatcatc
    41161 cagctacttt ccaaaaaaaa gatgaatgtc aaagatttgg caaaagaact tggagtgagc
    41221 agtgctatca cgacgatgca cgtgaaaaag ctagaggaag caaacatcat caaaacagaa
    41281 aaagtaggac aacaaaaaat ttcgagcctt cgtgtcgata aaatcgatat tagttttcca
    41341 gaaaaaatat tcaatgcttt tgatacgaaa gaaacatcga tacctattgg tcactataca
    41401 aattatgcaa ttgaaccaac atgcggttta gctacgatcc atgattttat cgggaaagtg
    41461 gatgaaccac gctattttat ggatcctcga aggatggatg cccgcattct ctggtttacc
    41521 agcggatttg tcgagtatca ggcacctaat tttttaaata cagaggatac attggagatg
    41581 ttggaagttt ctgtggaaat ttcttctgaa tttccttttt caaatgacaa ttggccttca
    41641 gatatcactt tcagcctaaa tggtgtggag ctaggaacat ggacgagccc tggagatttt
    41701 gctgatatcc gcggtaaata tacgcctgat tggtatccag ataatcttaa tcaatacggc
    41761 ttgctgaaga cgatccgaat taccaaacac ttgacgaata tgaatggaga acccttgtca
    41821 aacatcacga tcaacgacat ccctaaagag caagacactt ggcatttgcg aatcgaagtg
    41881 aaagacgatg ccaaacatgt ggggggctgt actttatttg gtaaaggatt cggtaattac
    41941 gatcaggata tcaaattaaa agtttactac agctgatatt tttcttttct attttataaa
    42001 acaaaaacag ccaatcccta tgaagggacg gctgtttttg ctttattgtt atttccagaa
    42061 atcatccaaa acggaaattg gcaaatgtcg tttatgttgt gtcttgttcc accatgcttc
    42121 gattttttct tgtgcttgag cgggaacttc ttttccttct agataatcat caatttcatt
    42181 atatgtgaca cctagtgcga cttcatctgc aatcattggt ttcccatctt caagatctgc
    42241 agtcggaatt tttgtgtata atttttccgg tgcattcaat gcttgaagca attgtttccc
    42301 ttgtcgtttg tccaaacgga ataatggcaa aatgtcggct ccaccatcac caaatttcgt
    42361 gaagaatcca gtaatgtttt ctgcagcatg atctgtccct aatacggctc ctgctctttc
    42421 tccagctacg gcatattgcg taatcatacg ttgacgtgct ttgatatttc ctttgttaaa
    42481 atcgctgatt cccacacctg cttcgcttaa tacttgtact tgtgcatcaa cagctggttt
    42541 gatatttact cgcagactaa catcgggctg gataaagtca agagcagctt ttgcatcttc
    42601 ttcatctgct tgttcaccat aaggaagacg aacagcgata aattgatagt tttcatcttt
    42661 tgtttcttca cgcatttctt ccatcgttag ttgcgccaaa cggcctgcta aagtagaatc
    42721 ttggccgccg ctgataccta gtacaaaagt tttaagaaac ggatattttt tcaaatatgc
    42781 tttcataaag tcaatacttt tacgtatttc ttcttttgga tcaattgttg gtttgacacc
    42841 taattcattg ataatttctg tctgcaggct catttattct cctcctatgc ttctgtatga
    42901 agttctgcta cgcttttttt cattcgttcc atgatagcca ttttatggtt ccagcattca
    42961 gtagacagat cgactgggta tttttgcgga ttcaagtctc gtttatactc ttcccatagt
    43021 gaatctagat tttcttttgt atattctttg atttcgttta gtgcaggtaa ttcgtaaact
    43081 ctttttcctt cgataaagat atcttgtaaa actgggcgag ctgtaaagtc tcgaacagtt
    43141 ttattgataa acgtatgcac tggatggaac atgaagatag cttcttcttc tcgagggtct
    43201 tcatcccata atgtcacgta gtctccttca gatttcccat caaaatttcg agtgatgcgc
    43261 cacacttgtt ttttccctgg agtagttact ttttctgcat tgcttgataa cttgatcgta
    43321 tccatcattt ttccttcatc atcttcgatc gatactagct tgaatactgc accaagggct
    43381 ggctgatcat aggctgtgat caattttgtc ccaacacccc atacatcaat tttagctttt
    43441 tgcatcttta aattcaaaat cgttgcttca tccaagtcat tcgaagcata gatttttgct
    43501 tccgtaaatc cggctgcatc taattgttcc cgaacacgct tagatatata ggccatatct
    43561 ccactgtcga tgcgtacgcc ttggaagttg attttgtcac ctaattcttt cgccacgcga
    43621 atcgcacttg gcacacctga tttcaacgta tcataagtgt cgactaagaa cacgcagtct
    43681 ttatgggttt tagcgtaggc cataaatgct tcataatcat ttccataaga ttggactaac
    43741 gaatgagcat gggtcccgct agctggtatc ccaaatatct tcccagcacg tacattgctt
    43801 gttgcatctg ctccgccgat atatgccgct ctagttcccc agactgctgc atccagttct
    43861 tgtgcccgtc tagttccaaa ttccaatagt ggatcttctc cgatgacaga tttgatacga
    43921 gctgcttttg tcgcgatcaa cgtttggaaa ttgaccatat tcaacagtgc tgtttctact
    43981 aattggcagt gcgctaaagg cccttccact tggatcaatg gttcatttgc aaaaactagt
    44041 tctccttcac gagcagaacg aactgttgct ttaaattcaa aattttgtaa atactccaaa
    44101 aaaccttcag gatagacttc taatccacgc aagtaagcaa tatcggaatc attgaacgtc
    44161 agattctcta aataattgac taaccgttcc aatcctgcaa aaaccgcgta tccgtggtta
    44221 aaaggcattt ctcgaaagta acattcaaaa acagcatgta aatcagctcg acctaattcc
    44281 cagtatgtct gcatcatatt gatttgatat agatccgtat gcagcgtcaa actgtcgtct
    44341 gggtaagtat tattcatctt gtattactcc tcttggcaag ttttcatttc ctacccatta
    44401 taacaaaaaa aattaatagg aaaagaaatc ttccattttt tctgaaaaag cacatgtttt
    44461 acggatattt cttgctaaaa atgaactcca tctgctaaaa ctatcctttc ttattggtat
    44521 ttaacctaaa cgagaaagaa tacttatttt attcaatgta tccgagacac gaagaaatta
    44581 gcgttttcta tttttcttac ttgataaaat aagtagattt tcctactaca atcgtagccg
    44641 ttaagatacc gatgattgaa caaatataac caaatgtctc tacaacataa tcaatggcaa
    44701 aagtttttga agcaataata cgtctttcct tttttgcttc ttatccccaa agaaacggat
    44761 ataacttatt atgtattgaa tttttttgga aaggcgaatt atgacaagat ataccttgcg
    44821 ttgaaaaaac tcgcggaaaa aatatcacac gtttttaaaa agatacaaag taagaaagca
    44881 gctgtcaata gaatggaaac aaaaatggcg agacatcaat cagtctcgcc ttgtttttta
    44941 ttgtatacat tagatatgga gcaggttacc ataaagatga ttattagtcg ttattaaata
    45001 gaatccttcg tatttttttg ttctatcact acaatatctt tatttggacg tttgaattgt
    45061 attccggcac gttctagctc ctgtttcact cctacatgga ctttgacatc ttctcgtgtc
    45121 atttttaaaa cgtgatgaat cgcatagtcg atttcttgcc ttgtgatttt tttcttcgta
    45181 ttgatcatca cgacttgctt tttatcatat gtgtctacca atagcagttg tgttcgatct
    45241 aacgaaaata atttgtaata attaatagcc gtattcccaa aaaagaattc cactacttta
    45301 ggataaaatg tcttaatatt taaattacta ttcacaggtg tttcgattag cttcatctca
    45361 ttcctctttt ctatatagtc gatttagatc caatcttaag ttatgttacc atctttccta
    45421 gcattaagga atcggttaca attgaacctt taaagacaaa aggatttacc ttttatcact
    45481 taatagtata acataatttc atgagaaaaa aatacaattt tttcttgacc atctcgttac
    45541 gtaaaggatt atgcttacat ttgtcaggag gaaaaataat ggagtacaca ataaagaaaa
    45601 tgtcagagat atcaggagtc agtccgagaa ccttgcgctt ttatgatgag attggactgc
    45661 taaaaccagc taggatcaat tcttcaggtt atcggattta tggcaaaaaa gaagtcgatc
    45721 gtctgcaaca catcttattt tatcgaacaa tggcattcaa gttggatgat atccaagagg
    45781 tcttagacaa tccatctttt gaccaccaga aagctttaat caagcatcag caaatgttgc
    45841 tggaaaaacg ggcacaaatc gataccctct tgacaactgt ccaacagaca cttgatatgt
    45901 acgaaggagg aagaaaaatg agtgacaaag aaaaatttga agggtttaaa caacaaaagt
    45961 tgaaagaaaa tgaagaatcc tatggacaag agattcgaca aaaatacgga aaaaaaaagg
    46021 ttacagcatc caatgaaaaa tggatgaata tggataaaga aacgtatgaa gaaatgcaaa
    46081 ctgttgaaaa gcagatgtta tttgatctca ctctttactt aaaagaacca gaagatgaaa
    46141 gcttagcaga ccgtatcttc caagctcata agaagtggtt atcttacagc tggcctgaat
    46201 atcagccaaa agcgcataaa ggactaggaa tgatgtacga agccgatgaa cggttcactg
    46261 catattatga cgagcgaagt ggtcatggtg cagcaaaagc tttaaatgaa attatccaaa
    46321 aaaaggcgta atcttttaca tgatccaaca aaaaagtaca cgtaaaatga cctaataaca
    46381 aaacagcaga cacccaagtt agatttttat gctaactttt ggtgtctgct ccattctttc
    46441 acaagttttt tcatcctact ttaaatatat tcatcgttga ataaacgtat aaggtatttt
    46501 cactttatcc tttttttctg taagaaacaa ttgaaatgcc atttctccgc aagcttccag
    46561 atggtggtcg atggttggga aaccaagaag ctcgctgatc agcaaattat cgcgtccaat
    46621 caccactggt gtcttatctt gcagatagct ttgcaagatg cctgctgcaa ccatatccga
    46681 atttgtaaag acaccatcaa ctccaagctc tttaaaaaag aagcctgctt gttttccttt
    46741 ctctgcatct atacaatccc aaaaaacaag tgattcatca aaagatggaa aaagttgatg
    46801 acatagctgc aaagtgattt ttgagttata acttagcctg ccacttcgcc ccaacgtgat
    46861 gcccaattgc tttatacctt gctgcttcaa ataagtaaga ccttctgtaa ttgattgttc
    46921 tcgatcaata aagacacagc ttgcctgttt tcccttgatt tcttcacaaa aaactagagg
    46981 tccatatttt tgatatgcca ataaatcatc tagtgtattg gcacgagacg taatgatcaa
    47041 tccatcgaaa gctttagctg caaattcttc aagatacctt cgctcttttt ctttctgata
    47101 gcccgtcggt aaaagtgtca ctttgtaatc ataatcaaac gctgcttcaa tgatcccact
    47161 gatcaactga tcaaaatacg gatgattggt ataaggcaga atcactccaa tgcttttcgt
    47221 ttttccataa cttaactgtc tggcatttgc attagggata taatcgactt ctttcatgac
    47281 cgcaagtatt tctttccttt tttcctcatc tacatatgga tgtccattga ttacacgtga
    47341 aatagtagaa actgaataac ctgtgagctt tgcgatatct cgaatgtttg ccactgctgc
    47401 ttcgccacct atacaaaaag attttcttgt aaatagtcta ccacagctgg accgcattca
    47461 aaaaactgat acatttttcc ttcatgcgaa aaactgatcc gctgcccgcc ccaatatata
    47521 tcttttgtca aattttcgat ttcattcatt ggtatttctt ttactatatt cctattcaac
    47581 ccttgaaaac tgtacaacgt taatacggat cccttgaact cgacaaacgc ataaaaataa
    47641 ttgttcgttg tgccatctgt tatctctaca taagtattca ttgcaagccc tccttttgat
    47701 actttgagta tagtgtatga aacgcgtctc atagcaagga gtttgttttg ccaa
//
LOCUS       DAZUKC010000012        47470 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000012 DAZUKC010000000
VERSION     DAZUKC010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.012"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(55..789)
                     /locus_tag="ACG80T_000629"
     CDS             complement(55..789)
                     /locus_tag="ACG80T_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFS4840305.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="ACG80T_000630"
     CDS             1116..1526
                     /locus_tag="ACG80T_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFS4840306.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="ACG80T_000631"
     CDS             1519..2220
                     /locus_tag="ACG80T_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFS4840307.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="ACG80T_000632"
     CDS             2220..3806
                     /locus_tag="ACG80T_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4840308.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="ACG80T_000633"
     CDS             3825..4046
                     /locus_tag="ACG80T_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFS4840309.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="ACG80T_000634"
     CDS             4043..4804
                     /locus_tag="ACG80T_000634"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFS4840310.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="ACG80T_000635"
     CDS             5184..5693
                     /locus_tag="ACG80T_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFS4840311.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="ACG80T_000636"
     CDS             complement(5768..6307)
                     /locus_tag="ACG80T_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFS4840312.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="ACG80T_000637"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="ACG80T_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFS4840313.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7320..8171
                     /locus_tag="ACG80T_000638"
     CDS             7320..8171
                     /locus_tag="ACG80T_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFS4840314.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8199..8834
                     /locus_tag="ACG80T_000639"
     CDS             8199..8834
                     /locus_tag="ACG80T_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFS4840315.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="ACG80T_000640"
     CDS             8854..9627
                     /locus_tag="ACG80T_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4840316.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="ACG80T_000641"
     CDS             10035..10832
                     /locus_tag="ACG80T_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFS4840317.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="ACG80T_000642"
     CDS             10810..11223
                     /locus_tag="ACG80T_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFS4840318.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="ACG80T_000643"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="ACG80T_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFS4840319.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="ACG80T_000644"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="ACG80T_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFS4840320.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /locus_tag="ACG80T_000645"
     CDS             16000..16560
                     /locus_tag="ACG80T_000645"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFS4840321.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="ACG80T_000646"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="ACG80T_000646"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFS4840322.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="ACG80T_000647"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="ACG80T_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFS4840323.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="ACG80T_000648"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="ACG80T_000648"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFS4840324.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="ACG80T_000649"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="ACG80T_000649"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFS4840325.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="ACG80T_000650"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="ACG80T_000650"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFS4840326.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="ACG80T_000651"
     CDS             21907..22485
                     /locus_tag="ACG80T_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4840327.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="ACG80T_000652"
     CDS             22537..23019
                     /locus_tag="ACG80T_000652"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFS4840328.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="ACG80T_000653"
     CDS             23136..23510
                     /locus_tag="ACG80T_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840329.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(24188..25540)
                     /gene="brnQ"
                     /locus_tag="ACG80T_000654"
     CDS             complement(24188..25540)
                     /gene="brnQ"
                     /locus_tag="ACG80T_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFS4840330.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
     gene            25687..26277
                     /locus_tag="ACG80T_000655"
     CDS             25687..26277
                     /locus_tag="ACG80T_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFS4840331.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(26405..27754)
                     /locus_tag="ACG80T_000656"
     CDS             complement(26405..27754)
                     /locus_tag="ACG80T_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4840332.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(27922..28662)
                     /locus_tag="ACG80T_000657"
     CDS             complement(27922..28662)
                     /locus_tag="ACG80T_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS4840333.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(28675..30267)
                     /locus_tag="ACG80T_000658"
     CDS             complement(28675..30267)
                     /locus_tag="ACG80T_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS4840334.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     regulatory      complement(30405..30579)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            complement(30835..31131)
                     /locus_tag="ACG80T_000659"
     CDS             complement(30835..31131)
                     /locus_tag="ACG80T_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840335.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            31307..32032
                     /locus_tag="ACG80T_000660"
     CDS             31307..32032
                     /locus_tag="ACG80T_000660"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFS4840336.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            32025..32867
                     /locus_tag="ACG80T_000661"
     CDS             32025..32867
                     /locus_tag="ACG80T_000661"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFS4840337.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            32870..33391
                     /locus_tag="ACG80T_000662"
     CDS             32870..33391
                     /locus_tag="ACG80T_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFS4840338.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(33644..34207)
                     /gene="def"
                     /locus_tag="ACG80T_000663"
     CDS             complement(33644..34207)
                     /gene="def"
                     /locus_tag="ACG80T_000663"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFS4840339.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            34458..34727
                     /gene="rpsO"
                     /locus_tag="ACG80T_000664"
     CDS             34458..34727
                     /gene="rpsO"
                     /locus_tag="ACG80T_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFS4840340.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            34915..37041
                     /gene="pnp"
                     /locus_tag="ACG80T_000665"
     CDS             34915..37041
                     /gene="pnp"
                     /locus_tag="ACG80T_000665"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFS4840341.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            37551..37817
                     /locus_tag="ACG80T_000666"
     CDS             37551..37817
                     /locus_tag="ACG80T_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS4840342.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            37961..39952
                     /locus_tag="ACG80T_000667"
     CDS             37961..39952
                     /locus_tag="ACG80T_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFS4840343.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            40306..41607
                     /locus_tag="ACG80T_000668"
     CDS             40306..41607
                     /locus_tag="ACG80T_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFS4840344.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(41737..43107)
                     /locus_tag="ACG80T_000669"
     CDS             complement(41737..43107)
                     /locus_tag="ACG80T_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFS4840345.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVNPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(43289..43612)
                     /locus_tag="ACG80T_000670"
     CDS             complement(43289..43612)
                     /locus_tag="ACG80T_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFS4840346.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            complement(43937..44545)
                     /locus_tag="ACG80T_000671"
     CDS             complement(43937..44545)
                     /locus_tag="ACG80T_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFS4840347.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            44875..45591
                     /locus_tag="ACG80T_000672"
     CDS             44875..45591
                     /locus_tag="ACG80T_000672"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFS4840348.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            45604..46299
                     /locus_tag="ACG80T_000673"
     CDS             45604..46299
                     /locus_tag="ACG80T_000673"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFS4840349.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            46383..47012
                     /gene="udk"
                     /locus_tag="ACG80T_000674"
     CDS             46383..47012
                     /gene="udk"
                     /locus_tag="ACG80T_000674"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFS4840350.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
BASE COUNT        15391 a         8788 c         9878 g        13413 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcaccggg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatggaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaac aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcacagca tgacagcatt
    23641 gggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cactagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagttt ttttgcgaaa actattttta tatcatatat atgtctaaaa tctatattat
    23881 aaaagaaatg ttgtttgatc agcttctctt cggtattagt tcaaaacaaa acggcaaagt
    23941 atatggagct actttgccgt tttgtttctc taatactcag gtcagtttat agacgctcaa
    24001 taaacggtgt tcaaaagcca gtttctcggt attaagtcaa atttatgcaa aaatgtgaga
    24061 agccattttc aaaaaatatt tcttaatact cgaaactgag cggctttttc acgacctctc
    24121 aataaacagt gttcaagaga cagcctatcg gcaataaggc tgaacgtctc tttcacaacc
    24181 tcttttatca atctgctact tcttctttct tagcgaaact ccaaatcagt ccgataacaa
    24241 aaccgataac tgctggtacg atccacccca tccccaattt gaaaaacggc aagtagcttt
    24301 ccgcaacata taaaagattt tgaacaatag gtgtttgttt gatagtttct gggcaagcgt
    24361 tcaacccatc aaaaatcgaa gcaatcaatg taaaataagt agtcattcga taaacagccg
    24421 tacgctgctt aaacaaagga ccaactaaca ctagtaaaat cagtgtcatt gctaatggat
    24481 agatgaacat caaaacaggc aacgaaaatt gaatgatatt cgtcaagcct acgtttgccg
    24541 ctaaacaagg aaggatacta gcaagtgcaa caaagaatgc gtaatttctt tgcggaaaca
    24601 aatctgtgaa tgtttctgca aaagctgtaa tcaaaccaat accggtcttc aagcaagcta
    24661 atataacgat aaatgctaat aagatactgc cataagtacc taaataatga tcagcaatct
    24721 gagccagcgt gatcccgcca ttttcactta gagcaaagct acctaagctc atagcaccta
    24781 aataagcgag caaagtataa atgattccca tcaatataat gctgatcgca ccagatttga
    24841 tcgtgtcttt agcgatttca gcaggttttg ttacacccat gttttggatt gttgtgacaa
    24901 tgatgatccc aaatgctaaa gcagcaagcg catctaatgt gttatatcct tgagtaaacc
    24961 ctgtgaaaaa agcatgctct tgataatttg gctgaatagg cgcttgattt acagaaccta
    25021 gtggattcaa aaaagcaagg agaagcaaag cacctaataa aaccaaaaat gcaggattca
    25081 ggaactttcc aacataatct aatatctttg tcggttttct tgaaaaccac caagccagca
    25141 caaaaaatac aatactgaaa attgctagat atagcgtctg tctgctagga tcgataaatg
    25201 gtgctaaacc aatctcgaaa gatgtggttg caaggcgagg taaagcaaaa aacggtccga
    25261 taactaagta aagcaagatg gtaaaaacca tcgcatatgt ttgatttatc cgttttgcta
    25321 attcatatac acctgagctt ttcgatacac caatggcaat cacccctaag aagggaagac
    25381 cgataccggt aaccaagaaa cctagattcg cccaaaaggt agcagcgcct gcttcttgac
    25441 ccaaatgaac gggaaagata agatttccag caccaaaaaa tagaccgaaa agcatggagc
    25501 ctataaaaag atagtcttta aatgataatt tttttgtcat acataactcc tttacggttt
    25561 ttttaaaagt aacaaaattt cgaaaaatat gcaacaattt tcagaaaatt tcctatataa
    25621 aacgacggtt atttaataac gtaagtgata aaataaaagc gctgaatcat ctgaggagga
    25681 atcatcgtga caaagtcgag attagaagca tttactgatg gagtggtagc cattgttttg
    25741 agagttcttg tattagagat caagatccct aatacatcta gctttcattc tttatgggtc
    25801 atccgaaata cattattagc ttataccatc agctttatat ttgttgcggt gatctgggtg
    25861 aaccatcatc gtatatttca gatggcagaa cgaattaatt accgagtggt ctggtcgaat
    25921 atctttttgc tcttttggct gactttatgt ccttctgtta ctagttgggt cgggcgtaac
    25981 cctgaagctt tttggcctga gatggcttat gtcttagtat acacgatgtg gagtttttct
    26041 tttggtatct tgatgaggca aatcatgaaa gcaaataaac cagattccca tgtcgtgaga
    26101 gttttgtcaa aggaccgtcg aagtcttatc tccatgatga ttaacttatg cttgattgcc
    26161 ggtgtctttt ttgtaccgtt gatcgggtta ttcggacgtt tttttgtatc tggtatttgg
    26221 attttttcct ataaaaaagc agatgcctat tatcagcggt tgtttccggg gaaatagaaa
    26281 agaggctggg acagaagctt ttaactccaa gaaataagcc gaaatttcac aaaaatttaa
    26341 gaagcaattt tcgtaaattc cttcttattt ctcgaagctg accacttctg tcacaactac
    26401 tttcttactt cttacgttca tatttcatat gatagattag taatagaagc aagaaccaca
    26461 caggtgttag taataaagcg attctcgtat cttttcctag gaacaagacg acacacacaa
    26521 aggccatgta aagaaatacc agataattac taaatgggta caacggcatt ttaaatgaat
    26581 gagctttttt gccgaggata cttttacgat atttcatgtg cgtgtagaca agcatactcc
    26641 agataaacaa gaaacaagta gtggcaatac tgcttactag tgtgaatact tcactaggca
    26701 tcacgtaatt caagataaca gaaatgaaaa tgacgatagt agaaaatgtc aaagcattcc
    26761 ctggcacatg atgtgttgtt aactttttga attttttagg ggcactgcct tcttgcgcta
    26821 aagaacgaag catacgtcct gtactatata ttgcactgtt gcaggcagaa gctgctgcac
    26881 ttaatacgac taaattgatc aaggaagcag caatcgtgat cccaatttcc gaaaatactt
    26941 ctacaaatgg actgttatct ggattcaagc tgttccatgg atagattgcc ataatgacaa
    27001 aaagagatcc taagtaaaat aagatgatcc tgataggaat attattgata gcttccggca
    27061 acactttttc aggattttct gtttctccag cgactaatcc aactaattct atcccagcaa
    27121 aagcaaaggt taccatttgg aaagctaaga taaatccttt tgcaccagtt ggaaagaatc
    27181 cgccatgaga ccatagattc gtgatactgg ctgtgccaat atctgtcgta tagtgggtga
    27241 ggatcatata agctcctacg aggataaagg caatgatcgc tacaactttg atcaatgcaa
    27301 accaaaactc taactcacca aacaatgaga cagccaccat attcaagcaa agaagaagac
    27361 ctaatgcaat cagttcagga agccactgag gaacaccagg cgcccagtag cgtacataca
    27421 ttccgatggc tgttaaatcg gccattgcaa tcgataccca gcagaaccaa tacgtccagc
    27481 cagtgatgaa agccgctttt ttgccaagat attctgcaac aaaatccaga aaagaatggt
    27541 tattggtgtt tgataacaga agttcgccta acgcacgcat aatgaaaaaa cagatgcatc
    27601 cagtaataag ataggcgagc aatatcgaag ggccagctag ctggatagac tttccggaac
    27661 ctaaaaacaa ccccgttcca atcgctccgc caatagaaat cagttggaca tgtctatttt
    27721 tcagtccacg ctgcagttct tgctcttcat tcatttttaa atcatcctaa ctaaaaaagt
    27781 ttaattactt ccatattaaa agagttttct gaaaaacaca actaagattc caaaattttc
    27841 agaaatttac cttttatgag taattcataa aaaagaactg cggtaaacac cgcagctctt
    27901 tgtcccaatc agctattgat cctaagcatt taaaatacgt gaaaggaatt cttttgtacg
    27961 ttcttctttc ggatggacaa agatatcttc tggtgtacct tcttctgcaa tcacaccttt
    28021 atccataaaa atgacacggt cagatacatc ttttgcaaat tccatctcat gcgtcacgat
    28081 aagcattgtc aagccagtat gtgctaagtc tttgatcgta ttaagcactt cgccaaccat
    28141 ttcagggtct aacgccgaag taggttcgtc gaatagcaat acatctggat tcattgataa
    28201 tgcccgagca attgcgacac gctgtttctg tccaccagat aattgtgctg gtttggcttc
    28261 gatataacga tccataccaa ctttttccag attttcgatt gctacttttt tggcagtttc
    28321 tttatctcgt ttcaggacag ttgtttgacc agacatacag ttttccaaga cattcatatt
    28381 gttgaacagg ttgaaggatt ggaaaaccat acctagattc gttcgatatt taggtaaatt
    28441 atatttagga gctaatacat tttctccatt ataaatgatc tcgccgcctg ttggtttttc
    28501 cagtaaattg atacagcgca agaaagtaga tttaccagaa ccggaagaac cgatgatcgt
    28561 cacaacttca cctttattga ctgttacgct gatatctttc aatacttcat tttcaccgaa
    28621 gctttttctt agatgtttga tttcaataat tttttcattc atgattcttc ctccttattc
    28681 ctgcaaattt gaattgtcga tttcttcaag cttggtataa gctgaaggac catcgatttt
    28741 cttttctacc gcccgcagga ttctggtgat cgtaaaggtc atgactaagt agatgatccc
    28801 gacgatcgta aatgtttgga agaattggaa attcgcaccg gaagcagagt ttccttggaa
    28861 gaacaaatca gctacgccga ttacacttaa aactgctgta tctttgatat tgataacaaa
    28921 ctcatttcct gttgctggca agatattccg taaaacttgt ggaataacaa cttttctcat
    28981 cgtttggcca tgggtcatac caatggcttg cgcagcttcg aactgccctt catctactgc
    29041 aaaaatcccc ccacgaacga tctctgtcat gtaagctcca gtattgagag agacaataaa
    29101 caaagcggca atcgttcggt tcaatgaaat gccaaaagcg agtgccagac cgtagaagat
    29161 gaccattgct tgtaccatca tcggtgttcc gcggaatact tcgacataga tagaaagaag
    29221 tgcatttccg attttttgga agaagcgagt cagtgcattt cctgattcag gtaccgaacg
    29281 gaagacacca atcagtaatc caagtgtagt accgacaact gtcccgatca atgcgatgaa
    29341 caaggtaagt ccagcacctc gtaagaacat attgccgtat tgtttccaaa tcgtagtgaa
    29401 atcagaaatg atgccaggtt tttcttcact atcagttgca gcaggctgat ctttgactgc
    29461 ttggtccata attttggttc gttcatcgtt agagatgcca gaaagaatct ggtttacttt
    29521 ttggatatcc gggtcgcctt tacgcatacc aacagaaatc tgaacatctt ccgggtttgt
    29581 ctggaagcca tctgcaccgc tgaattcaag catttccaag tctttgttca cactagttgc
    29641 cgttacacct tccggacgtt cgctgacata accatcgatc attccagaag ctaaagctgt
    29701 tctcattgct gagaaattat ccattgcttg ttgtttctgc acatctggta tctgatcgat
    29761 cacagtatag tggaatgtat ttaactgagc agtaatcttt gctccagaaa gatctgctaa
    29821 actttttgct tttgcatatt tgctatcttt ttgaactact actactaatt gtgattcgta
    29881 ataaggatcg gtaaaatcaa tttcttttcg tcgttcagca gtagggctca ttccggcgat
    29941 gatcgcgtcg atcttgcctg attgaagggc aggagccaga ccatcccatt tcgtttggac
    30001 gatgactaat tttttcccta aaccatctgc tactttttta gcgatttgaa catcataacc
    30061 gccggcgtaa ctattttggc cttgaattgg caaggcatta ttctcatctg tttgttgaga
    30121 ccagttgaag ggtgcatacc cagcctccat acctacgcga aactcatcgc tcggtgtttt
    30181 ttcatctgca tttgccagag atcccacact gaatagtgag aagacaaaag caaatagtac
    30241 ggcaactgaa accatatttt ttttcatttt tttctccctt tctgattata tgatacacga
    30301 gcggtctaga tgaaatgcgg taaagcttta tctgaaaaaa taaaaagcga ccgatttata
    30361 gatataaaaa ggccgctaaa attccataag gtgataaacc tctcacaaaa catagcgcaa
    30421 cttagcatca ctgctaagac agtccgcaag ctcttaactt gcgtcccaac atacactcca
    30481 agcaaaaaag tgcacatttc ggcgatcctc cctattcctg cttctccatg tttgcttcac
    30541 tccacagggt taatgattct cgcgacctct acctcattga ttgaggcatc tcgtattcag
    30601 ttatttatac aaaaaaagtt tacgatactc gagaggcttt gtcaatctat aactgctgtt
    30661 ttttgaaaaa atactgataa atatagaaat ctttactttt accgaaaaaa caatcaaaat
    30721 gtgaatcatc tatccattgc aaacgatagt ataattatac acaaaaatgt atgtaataca
    30781 ataaaaaaag aactagaaca gaaaatttcc tgtgtccagt tccatctcga tcatttaagc
    30841 tgccgctttc tttggctcac gtcctaggat cgcttgcaaa agaacaacga cgccataaat
    30901 ggctaatatt agataaatca aacttgagta ttgggtgatt ccccagcccc atgttcgata
    30961 tacggctaat gtaatggcta aaacaattgc ggcaatcacg ttgaataaag caaaagccca
    31021 aagatttcca ttttctttgc gtacaagata aaaaacagag aaataaacgc ttaacgctaa
    31081 ccaggcgata caagccagaa taacgcccca ataaataaag aatggtaaca tatcgattca
    31141 cctcactttt atacagcagt attgtaacac gttttcattc ttttgtcagt gaacattgtg
    31201 ataaattgtt caaatgccca taacactttt ttattcccat ttttctgact tttcaacaat
    31261 aaatttgttc gagtatgggc tataatggaa agtgaggtga caaaacatga aacaattaac
    31321 agtagacgaa gcagcaaaag agatattagc ttatcagcaa attacatttt tgaccggtgc
    31381 aggggtatcc acaccttctg gagtgccaga ctatcgatct ttgtcaggaa tctaccacgg
    31441 gatggagcag ccggaatact tgctgagtca tcaagcaatg gtgaatgaac cggataaatt
    31501 ctatcaattt gtcaaaaaga tttatcatcc tgaagcaaaa cccaatatca ttcatttgga
    31561 aatggcacat ctagcgaaag aaaaaaatgt ttgggttgtt tcccaaaaca tcgacggttt
    31621 gcacgcaaag gctaaaagcc cgcaactcgt caattttcat ggaagtttgt atcaatgtca
    31681 ttgtcgaaaa tgtcaacaaa cggtagactg gaaagaatat cttcacagcg acaaacatca
    31741 agcatgcgga ggacaaatac gcccagacat cgtgttatat ggtgaagggt ttcaagatgg
    31801 agtcatggag caggcggctt atgccgtcag ccaagcagag ctgatcgtca ttgttggaac
    31861 gagttttcaa gttcatccgt tttgtgatct ggtacagttt cgccagccgt cagccaaaat
    31921 cttagtaatc aatcaaacgc ctgtttattt agcggagcct tatacgttta ttcaagaaga
    31981 cggagcggtt gttttcaaaa aaatacaaga attaggagca gattatgact gaattcaaaa
    32041 ccgaaaaaga aaaaatgatt gcaggcgagc tttattttgc caatgatcct gagctggttg
    32101 ccgaccgtat gtttgcaaga agtcaaagcc aaatcatcaa ccaagcagaa tcagcggaac
    32161 tgcgatccca attgttgaaa gagacttttg gccgtacagg gaaaaaaatc tatatggagc
    32221 cagttatcaa ttttgattac ggatataata tatttgttgg ggagaatttt tatgcaaatt
    32281 tcaattgtac atttttagat gtcagcacga ttgaaatcgg tgataactgc atgttcgctc
    32341 cgaatgtcca gctttacacg gcgacgcatc cactacatcc agtgaaacgt aacagcggtc
    32401 tggagtacgc aaaacccatt aaaatcggta ataatgtttg gttaggcgga ggagttatcg
    32461 tcacgccggg tgtcactcta ggggacaacg tagtagtcgg cgcaggttcg gttgtgacca
    32521 aatcttttcc cgacaatgta gtgattgcag gaaatcctgc tcgtatcatc aagacagtag
    32581 aggaagaact aacagaagag tctcttgaaa cattacgtca taagatcgat atgatcgatc
    32641 gagagttgat tgctttatta gaaaaaagaa tggatacggt aactaaaata ggacaagtga
    32701 aaaaagaaga aaaccaagca gtttatgatg caaaaagaga gcagcaagta ttggataaag
    32761 ttgttagtct gctgaaaaat aagggatata aggaaacaat aacagatacg tatgttgatt
    32821 tgatgaaaca ctcgcgcgaa taccaaaaca aaatgaagga ggaataagtg tgatcgaagg
    32881 agagacacgg cgaaaacaaa tcatgcaaac cttggcaaat acagaaaaac cagtgagtgc
    32941 tagcaagttt gcgaaaactt ttggagtcag ccgtcagatc attgtaggcg atattgcttt
    33001 actacgagct gcaggtaaaa gtatcgtggc aacagcaaga ggttatatcc tcgaaacaga
    33061 agaaaccaat actgggcaca tcagtaaaat cgctgtccag catggaaaag atcaaacaga
    33121 agaagaactg agactaatcg ttgaaaatgg tggtgaaatc attgatgtaa ttgtggaaca
    33181 tcccctttac ggtgagttag caggaacttt gcacatcaaa acgccacaag atatcgattc
    33241 ctttatgaga cgttatcaaa aaagtaatgc tactttgtta tccgaattaa caggcggcat
    33301 ccatctgcat acgatccgct acccagaaaa gcatatgctg aaaaacatca aacaaaaact
    33361 agctgaagca ggaatcctct acgaaggata acatcgtagc tgctactttt tatcctagaa
    33421 actattctat catctctgag ataaacaccc atatggaaga aataagaaaa aataagaaaa
    33481 gatgcgtatc caatacacag aattttttgg aattgcgatt tctagatgtc tatctaaatt
    33541 tcgattagtg gagaaacata aaaaagggaa gtgcagcttt tgagcactcc ccttttttat
    33601 aaagctttct tgtagcatag tatcgaagga acctgccaat tctctactcg atcaccaata
    33661 cgccttcttt tagtgcaaat ggattttgat cgttgatgtg gtcatagaac atcacaccgt
    33721 tgatatggtc gatttcatgt tgaacgacga tcgattcata attcttcaaa cgaatcttgt
    33781 gtttttcacc attcatatca taataagaca cagtgatttt ggcatggcga acaacatatc
    33841 ctggtacttc tcgatcaaca gataaacagc cttcaccttc ccctaaacaa gcatcttgaa
    33901 cagaatggct caaaatttta ggattataca taacagtact tagagaaggt ccatctgcct
    33961 ctgggtcagg acttggtacg tgaacagcaa tgattctttt agaaatatct aattgaggag
    34021 cagctaagcc aacaccgcct cgtaagtgta gttcttcagc tttgattgga tcttggctgt
    34081 tttttaagaa ctcaagcatc tcttttccta aagagatatc ttcttcgctt aatggaagag
    34141 aaacttcttt tgcaacttcg cgtaaggtcg gatttccttc acgaatgata tcatccattg
    34201 taatcatgga atatgtaccc ctttcttaaa agactgtgtt tcaagtctat tttcgcatta
    34261 tagtttacca caaaaatgga aaagtagtag taagattgtt tgaaaactac cttttcttaa
    34321 tgattaacta cttgattttc aacccggttt caggtaaagt atagctgtgt gctctaggca
    34381 catgacctgc cacttggtta agcgaatcac caacgtttta ctcagtcgca ggggaataat
    34441 cgaaagaggt gaaagaaatg gcaatttcaa aagaacgcaa gaatgaaatc atcaacgaat
    34501 acgctcgcca tgaaggggat actggttctc cagaagtaca aatcgctgta ttaactgaag
    34561 aaatcaacca cttgaatgaa catgcacgcg tgcacaaaaa agaccatcat tcataccgcg
    34621 gcctaatgaa aaaagttggt caccgccgta acttgttagc ttacttacgt aaaactgacg
    34681 ttcaacgtta ccgcgaattg atcaaacgtt taggcttgcg tcgttaattt tagtcaaaaa
    34741 gagtgggatt cacatgaatc tcactttttt tactcgtata tcaattaagt aaaattccgt
    34801 gatcaagcta gatggactcc tactaaccaa atgaaagctg ctcaaaagac ggctttcggc
    34861 agttttcatt tgtttagtag cgtcttgctt tcacgagaag gaaaaggaga agttatgtca
    34921 gaaaaacaag tattcaaaac gacatggggc ggccgtccct tacaaatcga agtcggtcag
    34981 ctagcaaaac aagcaaacgg agccgtttta gtccgttatg gagatacagt agtcttaagt
    35041 gcagctgtgg cctctaaaga agcaaaagat acagactttt tcccattaac gatcaattat
    35101 gaagaaaaaa tgtatgcagc agggaaaatc cctggaggtt tcatcaaacg agaaggccgt
    35161 ccaagtacgg aagcaacatt gactgcccgt ttgatcgacc gtccaattcg cccgatgttt
    35221 gcagaaggct tccgtaatga agtccaagta acgaacatcg tgatgagtgt agaaacagac
    35281 tgctcgccag ctatggcagc aatgctaggt tcttctttag cactatcaat ctcagatatc
    35341 ccatttgacg gcccaatcgc aggcgtagaa gttggtcgtg taaatggcga gtacgtcttg
    35401 aatccaacag tagaacaagc agaacaaaca gatatcgaat tgactgttgc cggaacgaaa
    35461 caagccatca atatggttga aagcggcgcg aaagaagttt cagaagaaga catgcttggt
    35521 gcattgctgt ttggttttga cgctatcaaa gagttagttg ctttccaaga agaaatcgtt
    35581 caagcagtag ggaaagaaaa aatggaagtg actttactgc aagttgatga agtattgaaa
    35641 aaagaaatct ttgatgcttc ttacgctaca atgaaagcag cagtaatgac tgaagaaaaa
    35701 cttgctcgcg aagataatat cgagcaggtg aagatcgata tccgcgaagc ttatgctgag
    35761 aaatttgcag gtcatgaaga tgaagatcac ttgttgaaag aagttaaaca aatcacagaa
    35821 gatcttgaaa aagatgtcgt acgtgaattg atcaccatcg ataagatccg tcctgacggg
    35881 cgtaaattgg atgaaatccg tccattgtca tcagaggtca gcttattgcc acgtgtacat
    35941 ggatcaggtt tgttcacacg aggacaaaca caagctttat cagcttgtac actcgcacct
    36001 ctaggagagc accaaatcat tgatggttta ggggtggaag tcagcaaacg attcatccac
    36061 cactacaatt tccctcaatt ttctgttggt tctacaggac gtgcaggctc tccagggcgt
    36121 cgggaaatcg gacatggcgc attaggtgaa cgtgcgttag cacaagtaat cccttcagaa
    36181 gaagaatttc cttatacgat ccgtttagta gcagaagtat tggaatcaaa tggttcttct
    36241 tcccaagcaa gtatctgtgc tggcacatta gcattgatgg atgcaggtgt accaatcaaa
    36301 gcgccagtcg ccggtattgc gatgggactt gtttctgatg gtgaaaacta tacgatccta
    36361 actgatatcc aagggctgga agatcatctt ggcgatatgg actttaaagt tgccggtaca
    36421 aaagatggta tcactgcttt acagatggat atcaagattc aaggaatcac agaacaaatc
    36481 ctaactgaag cattaactca agcaaaacaa gcacgtatgg aaatcttaga agaattgact
    36541 tcaacgattg ctgctccacg tgaagaatta agccaatatg caccaaaaat cgaaatgatc
    36601 cagatcgaac ctgctaaaat caaagatgtt atcggtaaag gcggcgatac gatcaacgga
    36661 atcatcgatg aaactggtgt gaaaatcgat atcgatcaag atggaaaagt aagtattgct
    36721 tcagctgatg cagaaatgat caaaaaagct atcaagatca tcgaagattt gaccaaagaa
    36781 gtcaaagttg gtgaagttta tctagggaaa gttgtccgta tcgaaaaatt cggtgcattc
    36841 gtcaacttga tcaaaggaaa agatggactt gtccatattt cccaattagc aaacgatcgt
    36901 gtcaataaag tcgaagatgt tgttaaacta ggtgatgaga tccttgtgaa agtaactgaa
    36961 atcgataaac aaggacgcgt aaatctgtca agaaaagcaa tgttgaacga agatggttca
    37021 acgaaaaatg aagaaaaata atttaattca taggaataat gtacttgtga tcgtgaaggt
    37081 gacaggggga caataattat tcgaaaaaag gggactgtga caaaagccgt gtcacagtcc
    37141 ttttttttcg aataaacggt gttcaaaagc ttgcatctgc ggtaataagt ccagccaagt
    37201 taaaaacatt aggagctgtt tttatttggc tgttcttatt acttgatgca gaacagcttt
    37261 tttacaacct ctccaataaa cgatgttcaa tcagctgtcc ctcgatatta gctcaaaaaa
    37321 cggcaaaata tgaggaacta tttttccgtt tgtttctcta atactcaggt caaaccgctg
    37381 atttcacaac ctctgattca cggtgttcca gaagcagctt ctacggaaat aagctgaaca
    37441 cttttgttac aacttctttt tttctggagt ttcttggcgc atatttttgg agtccttctg
    37501 aataaagcgt aaaataaaag tgagaaatat ggataaagga gggaaaggag atgctgaatt
    37561 ttttatggtc gttatttatc ggcggtatat taggtttgat agctggcgca attttaggaa
    37621 aggaagtccc gggaggcgta tctggaaata tcattatcgg ttttttaggt agttggctcg
    37681 gcgaattttt actaggacca ctgggaccgg aactcggcgg tttttatttg ataccagcat
    37741 tattgggtgc aattttttgc cttgctcttt attcatttgt ggcaagacgt atcagagaac
    37801 gtccaaagaa agaataattt tattcaaata agttgacact tcagtgtgtt acttggataa
    37861 ttagaagcaa tttaggaaag cgggtgtcat ggataagaat atcatctatc attcaagctt
    37921 acctaaagga tatccatata acaagaaatg agggaaaaac gtggcaacaa cgcacaatac
    37981 caaaaagata tcaatgactg gcctgcttgt agcgatggga gtcgtctacg gagacatcgg
    38041 gacaagtccg ctttacgtta tgaaatcgat tgttgaagat aatggcggat tgtcaggaat
    38101 caatcctgat tttgttatcg gatcagtctc attgattttt tggacattga ctctactgac
    38161 aacaatcaaa tatgtcgtga ttgcattgaa tgcagataac catggtgaag gtggaatttt
    38221 ctcactctac acacttgtcc gaaaaggtgg gaaatatttg atcattcctg caatgatcgg
    38281 aggagctgcc ttactagcag atggggtatt gacccctgct gtgacggtaa cgactgcgat
    38341 tgaaggcttg cgaggaattc cgcagttttt tgaccggttc ggtaataacc agaacgtgat
    38401 cgtcattatc actttagtta ttatttttgt tttattcatg gtccaacgct ttggtacaga
    38461 tattgtcgga aaagcattcg gaccaatcat gttcgcttgg tttacttttt taggcgtaat
    38521 gggattaatg aacttcagtc aagactggac agttatccgt gcactgaatc cttattatgc
    38581 tatccagctg ctttttagtg cagataacaa attaggcttg ttcattcttg gaaatatttt
    38641 cctggctacg acaggtgcag aggcgcttta ttccgatctt ggacatgtcg gcaaacgaaa
    38701 tattcgtctg agctggcctt acgtcaaaat ttgtttggtt atcaattatt ttgggcaggc
    38761 tgcgtggttg ttgaatgttt atcaaaaccc tgaagcacag caaatcaaga acctgaatcc
    38821 attcttccaa atgatgcctc aaagttggat cgtgatcggc gttgcttttg caacagtcgc
    38881 tgcagtcatt gcttcgcaag cattgattac aggttcgttt acgctagttt cagaagcaat
    38941 caagttgaaa ctgctgcctc ggttaaagat catgtatcct gggaacagta tcgggcaaat
    39001 gtatatccca gcagtcaatc tgattttatg gatcgcctgt tctctagtgg ttgtcacatt
    39061 ccgaacgtct catcatatgg aagctgctta cggtctatcg atcactgtga cgatgttgat
    39121 gacaacagca ctgctttact tttatcttat ccaaaatggg cattctcgtt ggcttgcata
    39181 tttagttacc tttttcttcg gtgcgataga agtcgttttc tttatctcaa gtgttgtcaa
    39241 attcttccga ggcggttttg tagccgttct gattggtttg atcatcttag tcgtgatgtt
    39301 tatttgggaa caagggaaca ttattcgcga atcgtcaacc gaggatgtgt ctttgagaga
    39361 ttatatcccg cagcttagag aattacgtga tgatacttct ttaccaatct atcagacaaa
    39421 tgtggtcttc ttggttcctg atatggctga agataaagtt ggccgacagt tcatctattc
    39481 gattttagat aaacggccta aaagagcacg agtctattgg tttgttcatg tggaagtgac
    39541 ggatgagcca tatacgaaag aatatcaagt agatatgatg gacactgatt ttattgtcca
    39601 agtgaatctg ttcttaggtt tcagggtcca acaggaaatc aacgtatatg ttcgccaagt
    39661 catccatgat ttgatgaaac aaggccgttt gccaaaacag ccacaaagat actctctgac
    39721 accagggcga gaagttggcg atttccaatt tgtcctgatt gatgaagtag tttccaatgt
    39781 gacaactcta ggaaaatggc aacgtcaaat catgcaggca aaactggcaa tcaaaaaaat
    39841 cgctacctcg ccagaaacat ggtttgggct agagtacagc gaagtaaaac atgaaactgt
    39901 tccgcttatc attggaaata ctcggaaaac atggttgaaa gagcggttgt gaaagtagcg
    39961 ctttgacctg aactaatgtc gaatgtcagc tacttgaaca ccgtttatca gaggtcatga
    40021 aaaagctgta ctgcaacaag cagtaagaac gagcagataa aaacagcttc tcatgttttt
    40081 catctgtttg ggcttaatgc cgtagatgca agcttttgaa taccgttttt atcagaggtc
    40141 atgaaaaagc tgtttagcaa caagtaacgc aatgattttg aagtgataag gagatgtggc
    40201 tgaaaaatgc cgcatctcct tttttattcc taaatatgaa tacgcattat tttcaatctt
    40261 tttgtagaaa aagggtacaa tagaaaaaga gaaaggcggg agaatatggt tatcaatttc
    40321 attattttat ttttgatgat tgcaattact tcattttttg tagcaagtga atttgcttta
    40381 gtgaaaatca gacgatcaag attggaacaa ctagaaaagg aaaatgtaaa gaacgccaag
    40441 ctagctcttc atgttacaca ccatttagat aactatttat cagctagtca attaggaatt
    40501 acgctaaccg gtttgatcat cgggtgggtc ggtgaaggat ctgtcgcggc tttactagaa
    40561 ccagtgatcg gtaaactacc ttttagttcg gctattagca gtacaatctc tgtggcgctg
    40621 ggatttattt tagttacgta tatcgatgtg gttattggcg aactgcttcc taagagctat
    40681 agtatcgtca atacggagaa ggtcgtcttg ttcgtagtta aaccacttca ttatttttat
    40741 aaagtaatgt ttccttttat ttgggtattg aatcattcag ctgcagggct agggaagcta
    40801 ttaggtgtgc gtctcgtatc tgaaggagaa gagacattgt ctcaagaaga gctgacatta
    40861 gtcgcattga attcttatga aaaaggagag ttgaccaaag aagaatatta ttatttggaa
    40921 aacgtgtttg aatttgatga taccttggca aaagatatac aagtagaccg tacgtccatg
    40981 caagtattcg aagcttcaga aaccgtcaaa gaagcgataa aagcttcgat tgaacaagga
    41041 catactcgct atccggttat cttagaatca aaagacaatg ttttaggtta tgtcaccttg
    41101 ccagatttga tcaagcaatc ttataaagat gaccagctga cagtagaaca gttgattgaa
    41161 gagccgatcg taacaactga aacgatccct ataaaaaaat tattgacgat catgcgaaaa
    41221 aagggtaagc atattgctat cttaaaagat gaatacggag ggacaagcgg cttagtgacg
    41281 attgaagata ttttagaaga aatcgtcggg gaaatccgag atgagacaga tttggacgaa
    41341 gcattgatag cggaacagtc agatggctct tatatcatct ccggcaaact aacattagat
    41401 gatttccagc gttattttca tgtagagatt ccagagtttg aagaaacgaa ttttactaca
    41461 cttgcgggct ttgcttctag ccgatataaa gaaataaaag caggaacaat cattgagatt
    41521 gcatcattcc gtttcacggt attagaatac cagcatgcac atatcgatta tttcaaagta
    41581 gagtccacgg aaagaaaaac agaataaaag aaaaaaggct gtgacatttt ttgtcacagc
    41641 ctttttgtta tctaaaaaac ctgttttcgg taaacgaaga agatattttc aataaaagtt
    41701 ttcattttta gccgcaacga tttcttttaa tcaaggctat cttgcttctg catgttctaa
    41761 tgtgaaatgt tcctcttcac ccatgtgttt caattgtttc atcgaacgca tataagagta
    41821 aagcaacacg gcaacagaac cgatccaagc taaaggagaa gctgctgctg ctcctgggta
    41881 tccccacatt cctgtaagaa cgatagctgc aaaagaacgc ataagcaatt ccatcatacc
    41941 ggcgatcgta gggatcttgc tttgtccaag cccttgaagt gtgtagcgta aaatgaacag
    42001 gactgctaag atccagtaaa gagacccatt gatattgaag taggtttgtg ctaattcaaa
    42061 tacacgtgtt tctgatgggt taacaaacaa ttgaacaaag aaatgaccga agaaaatgac
    42121 gatagcacct gctaagagac taaatccgac actcatcagt aaagtttgac gtaccccttg
    42181 caagattcgg ccatattctt tcgcaccata gttttgtgca gtgaaagttg ccattgcaat
    42241 accaaatgac atcatcggct gatttgcaaa ttggtcgata cgaccagcag cggcttgagc
    42301 agctacgaca tctgtcccta gactatttag tgctgcttgt aacacgatag caccaacagc
    42361 gatgatcgaa gattgaaacg ccattggcaa agcagcattt aggtgtacag caaactcttc
    42421 tttttcaaat ttgaaatctt ttttgcgtaa ctgtaacaaa ggcatttttt tcttgatata
    42481 cactagacac agtacgctgg aagaaacttg tgcgattact gtagcaatag ctgcaccttc
    42541 cacacccatg ccaaaataga taataaagat caaatcaagt acgacgttga tgatgaccgc
    42601 aatgatcaga aagaatagtg gtgtgcggct gtcaccaagt gcgcgaatga cattagaaag
    42661 caaattgaaa ctgactgatg caaagatccc taataaaata attgaaataa atgtttgtgc
    42721 ttgatcaagt atttcaggag gagtttgcat caattgaagc atcggacgaa taaacaggag
    42781 acttaggaca gttaaaatca cagtgacgac aagactaatg acaacactag cagcaaaact
    42841 tttcttcaga ccccgataat cttttgcacc gtaacgttgt gcggtaataa tggctaaacc
    42901 agctgttaaa ccttgggcaa agccaatgat caaaaatgtc aggcttccag ttgcaccaac
    42961 cgctgccagc gcatttttac ctagcgtctg acccacgatg atcatatcga tcatattgta
    43021 gaactgctga aaaacatttc cgatcagtaa aggaacagtg aacatcaaaa tcagtttagc
    43081 ggggcttcct tttgttaaat cgcgcatctt ttaaactcct tatatcttta aaatttagtt
    43141 agtagtatat agcgtttatc ctgagaatac caattagaaa tcaatgataa tttatgaaac
    43201 tccataaaaa gaagccaaga attgagtcaa agaatcaaca acttggcctg gtctgtaaaa
    43261 taagggacat ccacgtttat ctatcacctt aatattgtct aatctcactt ggtaaaagga
    43321 tcgtttctct cgtaccgtca tcataggcgt atacttgagc aggaaattgg ttcgaatagg
    43381 caaacggcaa atcaatatcc atttccacat tgcttcctgc ttgagaaaaa tgcatcgtca
    43441 cttttccacg attttcgatc aaatcaaagg ttaatatatt ttctaatgga atgactccct
    43501 taagatctag gtcaataata aaccatatac tgtcaatcaa ttcatctggc aaacttgata
    43561 cgacaccaac tgaagcgtaa cggttgcggc tgctatcaaa actttcaaac atctgtttaa
    43621 cctccttcga atcaacgaat gttttatttt tataattaat cctaggataa actagaaata
    43681 cttacttcaa cgagggttca ttctaataaa gaatcaggaa aaatgcaaca ataattgaat
    43741 actttttttc aaagtataaa tttttataaa ggtacgataa aagtatgcga ggaaaagctt
    43801 ggtaaattag gatttttcgt ttgtaaagcc atttacaaaa gtggaagccc catatgaaaa
    43861 taaacgatta gaaataaaaa aggcgtgtta aaagctgctg acccgccgct tctaacacca
    43921 cctgattgtt tcagtattat tcttctgttg aaccgatcac ttctggttcg ccaccttcac
    43981 ctgtgctttc ttcttcttca ctttgagcga ctacagcagc gatttgctct tcactgtcgg
    44041 tcacgaattt gtaatcgtcg ttttttggaa gatcggctac tgtaatagaa tcacctaatt
    44101 ccaagtttgt cacgtcaact tcaattcgtt caggcaattt gtctggtgta gcagatacga
    44161 ctactgtata caagttttga gctaacactc caccagattt agcacccact gattcgccaa
    44221 caagaacgac ttctgtttcg acttctgttt cttccgacat gtttacagat agaaattctg
    44281 catgttcgaa atgattggta aaagtgttcg tttggatctt gtgaaccaat gtattgactt
    44341 tttgtccatc gatgtccatc gtgatgactg tgttcgctcc atgttcacgc aacaatttgc
    44401 ttagtgtcaa accatcgaat gagatagggg tactttctac tttataccca taaacaacag
    44461 ctggaacttt cccttcgtga cgaagtttgt ttcttaatga acggggacgt acttctcttt
    44521 tacttacttc taatgatact gacataacca aaattcctcc ttaaaatgtg actgtctgct
    44581 ttggtaacgg acagatttta tttaagtgaa ttctaaaggc taatctcgtg cacatcatcg
    44641 aaggcagggc gcttaaaaaa gcgcaaaagg agagatgcgc gcatctcccc ttagaaagtc
    44701 aggattcaac ttttctccac caggtcatta cgctgtgtat gagaaatcca attcacttta
    44761 ctatttaacc gtaacgtgaa ctgttaatta agtcaaggaa taactatgat gatgtatttg
    44821 caagcaagaa tattcaaggt ataattaatt agtagtaata agaaaggaaa cagcatgcgt
    44881 ttagataaat taatagaaga aaaattagaa acgacacgca aggaaatgaa gcggctgttt
    44941 gctttgaagc aagtgatgat agatggcaca gtcgaatttc gacaaaatcg aaatgtagat
    45001 agtttactgc atcaaatcga agtagcaggt gaacacttag aaacaaatga gtcctattat
    45061 cttctcaata aacctgaaaa agctgttaca gctgtatcag atccagaaaa gacgacagta
    45121 atcgatctga ttgcaagtgc cgatcgtttc caaccattat atccagtggg aagactagat
    45181 agagatacaa caggactatt gctgatcact tcgaatggac aattgggata tgaaatgcta
    45241 cttccagaaa aaaaggtttc aaagacttat caagtagttg tgaacgaaag ggtgacaccg
    45301 gcagacgtaa cagcatttga aaatggaatc gtattcgctg atgggtatca atgtaagccg
    45361 gctgatttaa aaatactcaa agcagacaag caccaaagta cggttttatt gacgatccaa
    45421 gaaggaaaat tccatcaagt caaaaagatg ttcttagccc aaggaaagaa agtgaccgca
    45481 ttgaaacggc taagcatggg accattggaa ttagatgaac agctaggagt aggcgagtat
    45541 cgatcgttga cattagcaga gatgaaaaag ttaagtattt attttaaata ggaggtcaaa
    45601 aagatgaaat ataaaacttt attgtttgat gtagatgata cattgttaga ttttcaatta
    45661 actgaagcca atgctttaag tgctttgttt gcagaacaag gaatcacatt gacaccagaa
    45721 atcgaaacca gctataaggc tttgaatcat catttatggc aggagtttga aaaaggcaga
    45781 ctatcacgag aagaaataat aggcagtcgt tttgggttac ttttccagca atttgggaaa
    45841 acagtagata gtgatcaaat ggaaaaacgc taccgccact acttgaacca aggtcatgat
    45901 cttgttccag gaagtctaga aatcctgaaa aaggtccatc cgcatgctga tctttatatt
    45961 gtgacgaacg gtgtgtcgca cacgcaacat cggcgattaa cagatgcagg aatgcttgat
    46021 tacttcaagg atatctttgt ctcagaagct gtcggagcgc aaaagccgat gaaagaattt
    46081 tttgatcatg tcttttccca cattcctaat tttgataaaa agcaaactgt gatcattggt
    46141 gattctttga cttctgatat caaaggagga aatatggcag ggatcgatac gatttggttc
    46201 aatagaaata aattaccaga aatcgaagaa atccagccta cttatcgaat cgattcatta
    46261 gaggaacttt attcattatt ggaaattgaa aaggattaat tttcagaaaa atatagcatt
    46321 ttcgtgtttt ttcgctataa tagattagaa tgaaaaaacg gatacgaaag aggttttagt
    46381 caatgacaaa aagcaagcca attatcattg gtgtgacggg tggatccgga agcggaaaga
    46441 caagtgtcag ccgtgcgatt ttcaacaact tcccggacca ttccatcatg atgcttgaac
    46501 aagattctta ttacaaggat cagagccatt tgagttttga agaacgattg aacacaaatt
    46561 acgatcaccc atttgccttt gataatgatc tgctgattca gcatgtaggc gacttactga
    46621 actataaagc cattgaaaaa cccgtgtatg actatgtagc gcatacaaga agtcaggcaa
    46681 cgatcattca ggaaccaaaa gaagtgatca ttctagaagg aatcctgatt ttagaagacg
    46741 aacgtctgcg tgacttgatg gatatcaaag tatacgtaga tacagatgat gacattcgta
    46801 tcattcgtcg tatcaaacgg gatatggaag aacggggacg cacattggac tccgtgatcg
    46861 aacaatattt gacagttgtt aaaccgatgt atcatcaatt tatcgaacca acgaagcgct
    46921 atgcggatat cattgttcca gaaggcggag aaaaccacgt tgctattgat ttgattacaa
    46981 caaaagtagc tagtttctta aaccataaat agtgcgcagc gaaacagttg aacggactgc
    47041 gaccgtttga aaagcaagga ttctgctttt tgaacagtca ccagccaact gtttttttat
    47101 ttataacaag aggttgtgaa aacagcatct tgacctaagt attaaaaaga agaaacggaa
    47161 aatagctgtt tatattttac cgttttttga gctaataccg aaggtcagct gattgagcac
    47221 cgtgaagtag aggttgtaaa aaagctgtct ttgcatcaag cgataagagc atccaaataa
    47281 aacagctcct cacgtttttc gttgggttgg gcttattatc gtagatgcaa gcttttgaac
    47341 accgtttaac gagaggttgt aacagaagtg gtcagatgca ataaataaga agaaacttac
    47401 gaaaattatt tctcgaattt gtgtgagatt tcgacttatt tttttgaaga agctcttttg
    47461 tcgcaccccc
//
LOCUS       DAZUKC010000013        46230 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000013 DAZUKC010000000
VERSION     DAZUKC010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.013"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(449..979)
                     /locus_tag="ACG80T_000675"
     CDS             complement(449..979)
                     /locus_tag="ACG80T_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFS4840351.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            1084..1635
                     /locus_tag="ACG80T_000676"
     CDS             1084..1635
                     /locus_tag="ACG80T_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS4840352.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(1788..2261)
                     /locus_tag="ACG80T_000677"
     CDS             complement(1788..2261)
                     /locus_tag="ACG80T_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS4840353.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            2399..3871
                     /locus_tag="ACG80T_000678"
     CDS             2399..3871
                     /locus_tag="ACG80T_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFS4840354.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            3868..4578
                     /locus_tag="ACG80T_000679"
     CDS             3868..4578
                     /locus_tag="ACG80T_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFS4840355.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            4930..5214
                     /locus_tag="ACG80T_000680"
     CDS             4930..5214
                     /locus_tag="ACG80T_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFS4840356.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(5495..5914)
                     /locus_tag="ACG80T_000681"
     CDS             complement(5495..5914)
                     /locus_tag="ACG80T_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFS4840357.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(5916..6500)
                     /locus_tag="ACG80T_000682"
     CDS             complement(5916..6500)
                     /locus_tag="ACG80T_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFS4840358.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(6547..8022)
                     /locus_tag="ACG80T_000683"
     CDS             complement(6547..8022)
                     /locus_tag="ACG80T_000683"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFS4840359.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            8203..8514
                     /locus_tag="ACG80T_000684"
     CDS             8203..8514
                     /locus_tag="ACG80T_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840360.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(8629..10965)
                     /locus_tag="ACG80T_000685"
     CDS             complement(8629..10965)
                     /locus_tag="ACG80T_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFS4840361.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            11107..11775
                     /locus_tag="ACG80T_000686"
     CDS             11107..11775
                     /locus_tag="ACG80T_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4840362.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            11778..12821
                     /locus_tag="ACG80T_000687"
     CDS             11778..12821
                     /locus_tag="ACG80T_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4840363.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(13058..13939)
                     /locus_tag="ACG80T_000688"
     CDS             complement(13058..13939)
                     /locus_tag="ACG80T_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4840364.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            14098..14826
                     /locus_tag="ACG80T_000689"
     CDS             14098..14826
                     /locus_tag="ACG80T_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFS4840365.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            14982..15299
                     /locus_tag="ACG80T_000690"
     CDS             14982..15299
                     /locus_tag="ACG80T_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS4840366.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            15292..15900
                     /locus_tag="ACG80T_000691"
     CDS             15292..15900
                     /locus_tag="ACG80T_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFS4840367.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            15955..16746
                     /locus_tag="ACG80T_000692"
     CDS             15955..16746
                     /locus_tag="ACG80T_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFS4840368.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            16884..17372
                     /locus_tag="ACG80T_000693"
     CDS             16884..17372
                     /locus_tag="ACG80T_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFS4840369.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            17423..18295
                     /locus_tag="ACG80T_000694"
     CDS             17423..18295
                     /locus_tag="ACG80T_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFS4840370.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            18471..18833
                     /locus_tag="ACG80T_000695"
     CDS             18471..18833
                     /locus_tag="ACG80T_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS4840371.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            18842..19594
                     /locus_tag="ACG80T_000696"
     CDS             18842..19594
                     /locus_tag="ACG80T_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4840372.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            19608..21410
                     /locus_tag="ACG80T_000697"
     CDS             19608..21410
                     /locus_tag="ACG80T_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4840373.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            21687..22727
                     /locus_tag="ACG80T_000698"
     CDS             21687..22727
                     /locus_tag="ACG80T_000698"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFS4840374.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(22779..24923)
                     /locus_tag="ACG80T_000699"
     CDS             complement(22779..24923)
                     /locus_tag="ACG80T_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS4840375.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(25280..26653)
                     /locus_tag="ACG80T_000700"
     CDS             complement(25280..26653)
                     /locus_tag="ACG80T_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4840376.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSSKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(26653..27048)
                     /gene="rbsD"
                     /locus_tag="ACG80T_000701"
     CDS             complement(26653..27048)
                     /gene="rbsD"
                     /locus_tag="ACG80T_000701"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFS4840377.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(27076..27993)
                     /gene="rbsK"
                     /locus_tag="ACG80T_000702"
     CDS             complement(27076..27993)
                     /gene="rbsK"
                     /locus_tag="ACG80T_000702"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFS4840378.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(28101..29114)
                     /locus_tag="ACG80T_000703"
     CDS             complement(28101..29114)
                     /locus_tag="ACG80T_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFS4840379.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(29195..29794)
                     /locus_tag="ACG80T_000704"
     CDS             complement(29195..29794)
                     /locus_tag="ACG80T_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFS4840380.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            29980..30681
                     /locus_tag="ACG80T_000705"
     CDS             29980..30681
                     /locus_tag="ACG80T_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4840381.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            30691..33402
                     /locus_tag="ACG80T_000706"
     CDS             30691..33402
                     /locus_tag="ACG80T_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFS4840382.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            33853..35481
                     /gene="treC"
                     /locus_tag="ACG80T_000707"
     CDS             33853..35481
                     /gene="treC"
                     /locus_tag="ACG80T_000707"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFS4840383.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(35541..36722)
                     /locus_tag="ACG80T_000708"
     CDS             complement(35541..36722)
                     /locus_tag="ACG80T_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS4840384.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            36974..38497
                     /locus_tag="ACG80T_000709"
     CDS             36974..38497
                     /locus_tag="ACG80T_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFS4840385.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(38566..39294)
                     /locus_tag="ACG80T_000710"
     CDS             complement(38566..39294)
                     /locus_tag="ACG80T_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFS4840386.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(39307..40587)
                     /locus_tag="ACG80T_000711"
     CDS             complement(39307..40587)
                     /locus_tag="ACG80T_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFS4840387.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVALIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(40728..41330)
                     /locus_tag="ACG80T_000712"
     CDS             complement(40728..41330)
                     /locus_tag="ACG80T_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFS4840388.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            41575..41781
                     /locus_tag="ACG80T_000713"
     CDS             41575..41781
                     /locus_tag="ACG80T_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840389.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            42093..42812
                     /locus_tag="ACG80T_000714"
     CDS             42093..42812
                     /locus_tag="ACG80T_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFS4840390.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(42893..43576)
                     /locus_tag="ACG80T_000715"
     CDS             complement(42893..43576)
                     /locus_tag="ACG80T_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFS4840391.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            43794..45980
                     /locus_tag="ACG80T_000716"
     CDS             43794..45980
                     /locus_tag="ACG80T_000716"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFS4840392.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT        14956 a         8208 c         9230 g        13836 t
ORIGIN      
        1 ttttttgctg ggagcattat ttcattttga agtcactaat ttaactgtgt gaatatgcag
       61 ttgttacaat ctttgataaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa
      121 cggcaaaata tgaggaacta tttttccatt tgtttctcta atactcaggt caaaacgctg
      181 atttcacaac ctcttgataa acggtgttca aacggctgct tctcggtaat aagcccacta
      241 gcctagaaaa tatgaaaagc tattttctaa ctagcgttct tattactcaa agcataacgc
      301 tgatttcaca acctcttgat aaacggtgtt caatcagctg accctcggta ttagctcaaa
      361 aaacggcaaa acatgaggaa ctatttttcc gtttctttct ctaatactca ggtcagaacg
      421 ctgatttcac aacctctctt tcacaacctt attttactcg tttataattt tcatcgaagt
      481 attttccgct acggatatca tctggcattc catcgtatgg ttcttcgcta ataacatctt
      541 cattgtctat acctgcatca cccatataag tgatcgtagc aaattcagga acaagtaatt
      601 ttggataagt ttcatatttt tcacgagctg cttccatttc tgcgatatgt tcgttttgga
      661 aagtaacagt gatttctgga tgttcttcaa cccatttagg gaaaaagatc attccgtgaa
      721 gtttcttttc atttggcata aagtccaatg ccacgtctgt ccctgttggc tctgtccaag
      781 tgattttgaa tacgccgtcc gtcaatttaa cgatatcagc ttcttggtct tttacccaac
      841 ggcctttgac cattccgcca tggatacggt aatcaacagt atggtcattt ttggcatacc
      901 attcatattc ccaaccattg tcatacgtat agataaagtg agttccaata aaatcttcca
      961 attctttaaa ttctttcatg tttaagcatc tccttataaa ttaaaacatg taataaacta
     1021 catgttttcg attactcatt tatgatagaa tggatttaaa gatttgtcaa ggagggaaaa
     1081 ctgatggcaa gaaaaaatac acttcgatat attttgttag ggttattaag taagaagaag
     1141 atgacaggat atgaattgaa tcaatcgttc aaaaatgaaa taggagaatt ttggcaagca
     1201 aaacatagtc agatttatcc tgaacttgca aaaatggaag agcaaggaat cattcagcat
     1261 caagtagaga tcacaggtga aaaattggaa aagaaagttt atgaattaac agaagaaggg
     1321 aaggaacaat tggcagagtg gattcataca cccaccaatg aactaccggt taatcgagac
     1381 gaatttgttc tgaaattgta tttcgtcaaa gacgtgaatg atccggcatt agtagaaatt
     1441 attcagcagc agcgtgacct tcatgaagaa aaacgacttc atctattaga gcgaaaaaaa
     1501 acgatctttc ctactgaaaa agaaaaacat gaaaattacg gacactacct gattttagcg
     1561 catgcgatca atcgggaaac agaatatgca aattggctaa cagatgtatt agaagaaagt
     1621 aaccgaaaaa aataaaaaaa tatccgaaca gtgatggaga attcctttct atatgaaaga
     1681 aacgcagatc accgttcgga tattcttatg ttcaaacagg ccttttttca agtatttatg
     1741 tagcatctac ttattaaaaa gcttggtact taggagatta gtggcgttta gagttctttt
     1801 aacaactgtt cgtagtcagc tttttcatcg acaggaagga gcttgtaagt taattcgtct
     1861 gtctcgttgt catgaatgat gtacatctta gcattctctt ctttgaagtt accgatttgt
     1921 aaaatcgtat agcgttcgtc gatcaattcg atatctttga ttgcttttga aaaaacttta
     1981 cggacatggt gcattttctt ttggcggatt tctgcctttt ctgcagcttt tgacaaagta
     2041 aatccgtcat ctaaatattt tttgatcagt tctactttta tgactgtagg taaacgatat
     2101 tttctaggac aattcgcatc agcttcgatc gaacgaatga atcccttttg ttcccaatac
     2161 cttaattgac gtggagacac acctacgata tcacttaatt cactgatgcc aacaaccaat
     2221 tgatcatttt ccaacaaacg tttaagggaa gccccaacca tttttgtcac tccttttgtc
     2281 ctcttgcttc ccttaattat atccgtatta acttatgtgt caagtaggtt gacaaatagc
     2341 gaatttagga ctaaagtatt ctagtagttt cagaaaaaat tcagaaagaa ggagaaatat
     2401 gagtaacaac caacccgtag atatttacgg aaaaccatat aatcggacac ttttagttgt
     2461 tgtcttatta attggtacat tttgtaccgt tttgaatcag accttattga cgacagcttt
     2521 tcctacattg atgaaagctt tcgatatttc agcgtctgat gtgcaatggt taacgactgg
     2581 tttcttgcta gtcaacggga tcatgatccc aataacagca tggctgatca ataaattcag
     2641 ttcaagaaat ttatatcttt ctgccatgac gatctttttg atcggaacga ttacttgctt
     2701 tacagcacct aattttagta cattactaat cggtcgtttg atccaagctt gcggtgtggg
     2761 agtatcgatg ccattattgc aaaatatcat gctatctatt ttcccgccag aaaaaagagg
     2821 atcagcaatg gggatgtcag ggatcgttat tggacttgct cctgctttag gccctacatt
     2881 atctggttat atcattgata actaccattg gcgtgacttg tttggtatgg tactgccaat
     2941 cgttgttctt gtcttggttt tagcattctt cctaatgaaa agtgttattc cattgtcaaa
     3001 tcctagcatt gatattttat ccgctatttt atcaacgatt ggattcggta gtttacttta
     3061 cggattttct agtgtaggag atgacggctg gggaagcttg aaagtaattg gtttcttagt
     3121 tgttggtgtg attttcatcg ccttgttctc atggcgtcaa ttacatttgg aacatccatt
     3181 cttggaactt cgtgtattca aatcatctac attcacgatt gcagctgttt tagctggtgt
     3241 aacaaatatg gcgatggtag gggcagaaat ggtcgtacca ttgtatattc aaaatattcg
     3301 tggagaatca gcattccatt ctggcttgat gttgttgccg ggtgcattga tcatgggggc
     3361 tatgatgcct gtgacgggtg cgattttcga taaatacggc gcgaaacgtt tagccatcac
     3421 agggatgttc attttgacag cggcaactgc accatttgct ttcttgacaa aaacgactcc
     3481 agttttatat atcgtgatcc tttatgcgat ccgtatgttt gggatctcca tggtcatgat
     3541 gccggtaacg acttctggta tgaatgcatt gccaaataac ttgatcagtc atgggacagc
     3601 ggtaaataat acattccgtc aaatcgcaag ctcgattggt acagctattt tgatcagtgt
     3661 gttgacgaat gtgacaaaag caaatatccc aagcaaatct gtattggata atacgccatt
     3721 agcttataaa gatcaggcaa tcaatgccac actgtcaggt taccatgccg cgtttatcgt
     3781 agcgatcatc tttgggattc ttggctttat tattgccttt ttattgaaga aaaaagaacc
     3841 tgttctaatg aaagcggggg ataatgcatg attattggag ttttgattgt cagcttgctt
     3901 gcttttgctt taacgaacgt gttcgctaaa aaaacatggc aaaccttttt atctttgatt
     3961 ttcggactaa tcttcgtcgc ttcactaagt ttgatcgtcg ctaatttgtc caatcacttt
     4021 ggaatggaaa aagtaacgga aacaaaaaca gaaaaaattg tttccagtgc tgattcccaa
     4081 ggagcagata tgcttttgta taaagcttta gggaatggaa aagaaaaagt gtatctttac
     4141 cgaacaaatg aaaaacaaga aaaaccaaaa gcaactggta cggataacga aacaaataaa
     4201 gtagaaaaga cagacggcga tgctgaaaaa gtgacgaaaa ctacttattg ggaatacaaa
     4261 aacgacatgt ataaattctg gttcaatatt gctgataaca atcacgaata tgacagccga
     4321 gtgaatacat tcaaaatccc agagacatgg gtagaattaa gtacggacca agcagccaag
     4381 ttagctgaat tagtgaagaa acagcaaagt acgatggaaa gtgaagcaaa agcttatgtt
     4441 caagacggta tggtaaaagc aatgactgaa aatccaaaaa tgagcaaagc agagcaagaa
     4501 caacgtacga aagccttagc tgctgaattc caacaacaag cattcgcaaa attagtgaaa
     4561 gaagcaaaag gcgaataata ttttgaacac catgtaggag gttgtgaaaa agctgctctg
     4621 catcaagcag taagaacaat cagataaaaa tagcactcca tatttttcat ctgattgggc
     4681 ttagtgccgt agatgcaagc ttttgaacac cgtttaagga agaggttgtg agcctgccgt
     4741 ttaactctga gtcacaagga gcaatttcaa aaaacagctt ctcatgtttg ttgactattg
     4801 tgacttgtga ccgaggagtt ggcaggagaa caccgtttat tcgaaaaaag gaactgcgac
     4861 aagacttttg tcacagttcc ttcttttttt tgctatgcta tactagatgt aaaaaatagg
     4921 agaaataaaa tgagggaaat ttattctttt gaatcaaaaa tctatgcttc cgaagtcggc
     4981 aagggcgggg catatattat ttttccgtat gatataagaa aagaattcgg tgctggaaga
     5041 gttaaagtcc aagcaacttt tgacaatgtg ccgtatgaag gcagtatcgt gaatatgggc
     5101 gtgaaaaatc cagatggttc agtctgctac atccttggac tacgaaaaga tattcgtaaa
     5161 aacatcggaa aagaaatcgg tgatattgta gcagttacag tgaagcaaaa ataaaagagg
     5221 ttgtgacaaa agacgtgtca caacctcttg ataaacggtg ttcaaaagcc agtttctcgg
     5281 tagtaagtca aatttatgca aaaatgtgga aacccatgtt tacaaaattt ttcttactac
     5341 tcgaaacgaa gcggctttat cacaacctct tgataaacgg cgttcaatca gctgtccctc
     5401 ggtattagct caaaaacggc aaaatatgag aagctatttt tccatttttt tctctcatac
     5461 tcaggtcata acgctgattt cacaacctct tctttcataa tggtttttca ccatctttat
     5521 aataaactgg ttctttgata tggaaatcta ttttttccaa ccaatctaaa atcttgtaac
     5581 ctaactcttc tgacatcgga aaagaggcgt tgatttgtgc attggagata tggtcaacat
     5641 ggataccgga agtgatcgta caacttcccg gaacggcagt tttgatgatc acagctattt
     5701 tttcggctaa gacatcgtct ttatggaacc gaccatcgtg gctagggaaa cgtacagttt
     5761 gcatctctgt atcacgagaa aatgtagtga ctgttccgat atgtggtgta tctccgcctg
     5821 ttaaagtgat caataagtct ttaccgatta ctttaacagc agcttggatc gtgaaaccta
     5881 gtttggttac ttcaactatc gtttcttttt ccatattaat caccctgcca acgttttgaa
     5941 tgctcattta caatttcaaa ttgatccaaa agtttcattg tatggtgatt aacaagatct
     6001 tggatcgttt gaggctgatt ataaaaagca ggaattggtg gtatgatttg cacacctagt
     6061 cgtgctaatt tagtcaagtt ttccaagtga atggcattta atggcgtttc acgaggaaca
     6121 atgactaact ttcgttgctc cttcattgtt acatcagcag cacgtgcaat caggttgtcg
     6181 cctaaaccaa cagagatacc tgcgactgtt ttcatacttg ccggtacgat gatcattcca
     6241 tcggtcaaaa atgatccgct ggcgacagca gcacctaaat ctttgtttga ataatggtaa
     6301 tcaaacaggc tttcgaattc agttagtgat aagtcggatt ccagctttaa gttctgtttt
     6361 gcccatggac tgatgattcc gtgtgtttct atcatcggat attcttttag tttcttcact
     6421 aggttgatgg cataaatggt gcctgaagcg ccacttacac caatcacgat ttttttcttc
     6481 cgttgtgcca attgttccat tttccgcact cctttttatg atgtcaaaac agttgcagat
     6541 cattcattat ttcaaatatt tttcccaatc ttctacttct tggaattttg cacgtacaaa
     6601 atgttctttc atatcgaaag gaactgtacc gtcaaagata gtttttgcac ttatcccacg
     6661 gaaacgaata gagccaggat tatattctgg taattctgat gggtccaatg gatggttcct
     6721 cattcctggg atcacgatga tatctttatc tccttggaaa cgagtgttga aagtccaaac
     6781 tacatcgttc atgtcgaaaa tatcaacatc ttcatcgact aaaatgactg tcttcaattc
     6841 tttgaaggct gataaagcaa gaatagctgc ttgacgctga ataccttcat ccgcttcgtt
     6901 ttctttacgg atttgcatga tggtcatcaa tttaccacca cctgcaggag gattgtagac
     6961 atttactact tttcctggaa tagctttatc gaccaaggca agaatacttg cttctgtagg
     7021 aataccggcc atagatacgt gttcttcact gggaccaatc gttgtttgca tgattggatt
     7081 gtcttttcgg tgagtaactg ctttgacttt cactacttgt acagctggat tagcatcccc
     7141 agtatagcca ggaaattctg gcattgcttt tcctgagttc gtgttgatat cttcttgcat
     7201 tgtttcattt ggcatgattt ctgcttcaat gatgaattca gcacgagcaa tacccacttc
     7261 atcgactgct ataccatcaa cgatttgaat aggttcgtta cgcaaagctc ctgcacacca
     7321 tagttcattg tagccaagtg gagtggtagg tggttcgaaa gtcgtgccaa tggcaattgc
     7381 tggatctaaa ccaatattga tagtgatagg cattggttta ttcaaggctt cgaattgttt
     7441 ttggaagtga ccaatgtggc gtccgccagg catgatgtac ataccgatcg tatctttatc
     7501 ttctagaacc atacgatgga tcgttacatc gctcattgtt ttttctggat ctgatcctaa
     7561 gactacaccc attgtgatgt atggaccagc atcttcagga gtatttgtag gtgcaggcaa
     7621 aatgttgcga atatcgaagt ctgcatcggt agctagatga acgacttctt gtgctggagc
     7681 gtctgctttg tctactttca caggtttgat tggattttct acagcgtctt tcaagaaacg
     7741 accaagtgtt cgataatcat ggtgcaaaat ttcacctaca cgtttacgac tagccattgt
     7801 tccgatgttc acacgtgtgc cggggaaacc tttgatattg ttgaaagtca tagctggtcc
     7861 ttcttgagtc ggacgtttta ctgtaccgcc agcaccgata taacggtaaa caccagataa
     7921 ttctgcttca ggatcgattt caatatctgt ttcgtgatat tgacctggaa gtgtttttag
     7981 ttcttcgatg acttttctta aatcatacgg ttgatctgtc atgagagatt cctccttagt
     8041 tgtctttacc tatctatagt aaaaggtgaa aaagaggaaa acaattcaac ttgttcggta
     8101 aagtattcta caatggaatg gatgcgtttt tttgataata gaataaacta tgatatactt
     8161 atcactaaaa gaaagcgcat acttagtgtt ggaggaagga aagtgaaaaa aatggaggag
     8221 tcatttccta aagcagtaaa agtggaaaat atagcgaata ttttgaaagt tacatttgaa
     8281 aacggagaag taaaatacgt gaaatctcat tggacagaag agataaccga tgccttgcaa
     8341 tttggaaaaa aaggaagagg aaaaagaaaa aatcttctcg cactttctcg taatatgtgg
     8401 attgggactg aagtaacgat tgaagcagat ggaacagtat tcataaatgg aaaagacagg
     8461 tatacgccag aagagttgtg gtacaaagga aagaaaagta taccagaatt ataaaaaata
     8521 attaaaaaaa acagcgcgaa acaaggaaca tattacttcc tcgtttcttg ctgttttcta
     8581 ctatttcagc gctcttatga ctcaaatcta aatgtggttg tcacaacctt attctgcacg
     8641 caaagcgata gcggcgtctt ttttcgctgc cattcgtgca ggaagatgac cgccaagcat
     8701 cgtcaatata gtagagacga tcactagaat aatcgcatgg acaggattta gttgagcaac
     8761 attttttaga tccgtcatgt tgtataagat gctattgata gggaacgtag ctagccatgc
     8821 aataaacaca cctaatatcc cagaagaaat acctaaaata cacgtttctg cgtcaaatac
     8881 gcgagtgatg tcttttttac gtgcacctaa agcttttagt acaccgattt cttttgtccg
     8941 ttcaatcaca gatgtataag taatgatact gatcatgatc atacttgtta ctaaagagat
     9001 ccctgcaaaa gcaataagga cataagtgat agcatccatc aatccgccag tcagttcagt
     9061 catcgttcca gctaaatcag tataaatgat tttatcttca tctgatttgc ctttattgta
     9121 atcatctaga taatccaaga tcttttcttt gtcttcaaaa ttgttcggat aaatcatgat
     9181 acttgaaggc aagctgtctc cacctaaata actaagcaat gtttgtttgg cattttcgtc
     9241 aactttttca gtagttaaga catttacacc actgtctctt tgagctttga cgatttctga
     9301 ttctttgttt tcgttgacga tttgagtcgt taattgatcg ctataagcga ttcctggaga
     9361 taagagattc attgtagaag aagatttcac gcgtaggatc cccgagatct tcaattcatc
     9421 agaagcattc tgatacatcg catcataatc tgtattaggg ataaaattcc cagttggaag
     9481 ttttgtatag taagtattgt tgtttactaa tttgaatgtt gtaccaacga tatcatcaaa
     9541 atcaagtttt tcgtcttctt tcacatcaaa tccaagattc ttcaatgcgt tgatattagt
     9601 attattattg ccgtctacga tcagcacgac atctgttgct gaggctggat aacttccggc
     9661 aagtaaagaa taattgtctt ttaaaaagtt ttctttgcta gtgtcaagct gtgtagggaa
     9721 acttgacact cccacacctg tcattgctga catcgtacta gataaagaaa gagattccgt
     9781 atcaggattt tgattcgaga agctgacagg ctgtactttg ccatttacat cacgtaaaag
     9841 attgattcct gtagtacggg taaatccgat attattactt aagttaggat cgatgtctgt
     9901 tacgtaatcg acgtatgttt gatcaatctt attcgtgtgc tgtgctttat cttcttcgct
     9961 gacttttgca gtaatcgttt tgctgtctgg ataatccgct ttgcttgcac ccaatccggc
    10021 atcgtcacga gtctggttag tcgttacctt agaaatagta atcggaaatt tcgccatcgt
    10081 ttcagcttgc gtgttatcga tttgtttttg gaatccagaa gaaagggaca gaacgatcgc
    10141 gattccgatg atcccaatac tggaagcaaa ggaggtcaag aaagtacgtc ctttttttgt
    10201 tcggatattg ttgaaggaaa gctttaacgc tgtccaaaaa ctcatctttg ttctgcgtaa
    10261 gttaaattga tcatcttttg gtcgttcgat gtgaggatgg gaatctgtta agatcttacc
    10321 atcagaaaat tcaatgattc gatctgcgta ttgatgggcc agttcaggat tatgtgtgac
    10381 catgatcact aatttttctt tcgataactc ttggatcaat tccatgatct ggatacttgt
    10441 ttctgtatct aacgctccgg ttggttcatc gcacaaaagg atatctgggt cattggctag
    10501 tgctcgagcg atggccactc gctgcatttg tccaccagat aactgatttg gctttttatg
    10561 catatgattt gtcaagccga ctcggtgcaa tgcatcttct gcccgttttc gtttttcgtc
    10621 tttagaaaca ccacttaatg tcatcccaag ttcaacgttt tcaataatgc ctagatgacc
    10681 aatcaagttg taactttgaa aaatgaaacc gatagagttg tttcggtagg catcccagtc
    10741 actgtcctta aagtctttag tagactttcc gttgattacc atatcaccgg aatcataatt
    10801 atctagtcca ccaatcacat tcagcatagt cgttttacca gaaccactag gacctaaaat
    10861 cgctacgaat tctttttgtc tgaaagcgac agacacacca tccaatgctt tggtggtggt
    10921 ttcgccgact ttataatatt tttttatatc ttttaattga agcataatca cccttctccc
    10981 gtattgaaat attcggtata ctaataagta tatcagaaca atgatcgtag tatacgaaat
    11041 aaatgttaac tgaagatgaa ctacaagaaa aaatctagaa atttttcaaa aaacagaggg
    11101 agatcaatga atcgtatttt agtagtagaa gatgatccgc aattacagtt gctttaccgt
    11161 tctgtgttgg aacgagcggg cttttcagtc atcaccgcat tgaatggaga ggatgcgctg
    11221 aaaaaactag aagcgacacg tatagaatta atcattacag atatcatgat gccgaaaatg
    11281 gatggctatg acttgctaga ctcattacgt agcaccagat ttgacacacc tgttctaatg
    11341 attacggcaa aggccgattt tgaggataag aaacgcgggt tcaaattagg agcagatgat
    11401 tatatgacaa aacccatcga tgtcaatgag atgatcctac gtgtggaagc attgcttcga
    11461 cgttcaaaga tcaattacag cagtagcttg ataatcggac aaacaaaatt agagctagat
    11521 gcttatacag tgttacaaga tcagcattcg ttgattctgc ctcaaaaaga atttctgctt
    11581 ctgtataaat tactgtctta tcccaacaag atcttcactc gccaacagtt gatggatgat
    11641 atttggggat tagatacgaa tacagaagaa cgaacgatcg atgtacatat caaacgttta
    11701 aggacccgtt ttgaaaagac ggaagatttt caaatcatca cagtacgggg actaggctat
    11761 aaggcggtta tttaaagatg aaaaaaaagc tattttcaca attatggatc tattttgcta
    11821 gtattgtctt catttcgatc ttagtgacac tgctgtgttt tctcggtttt attttcttat
    11881 tgtctcgaca ttctatctcg cctgctgaat cggctggaaa accaacgtta tacccgttat
    11941 tcgtctttgc ggggttcagt atggtcgtgg gtacaggaat atcgattttt gttgggcggc
    12001 gtatcctgca tccaatcagt gcgctaggga caaatatgag tctggtagca acgggagatt
    12061 tttcgattcg aatggatgaa cagcagaaag tagcagaagt acagcaactg tataaagatt
    12121 tcaacgttat ggtccaagaa ctcaatagta tcgagacact gaggaatgac tttgtgtcca
    12181 gtgtatccca tgaattcaaa acaccactag caacaatcca aggatatgtt caattgttac
    12241 aagccccaaa tctctctgac gaagaacgcc aaatattttt atatcggatt atcgaaagta
    12301 tcacgcagct ttcacaatta acagagaaca tcttaaaatt gaacaaactg gaaaaccagc
    12361 gtatccaact ggaaaagaaa gagtatcgat tagatgaaca aatcagagaa gtaatcgttt
    12421 tttttcagcc taaatgggag aaagaacagc tagaattgga tatcgaactt gctgcagtaa
    12481 attacacagg caatgaagag tttttgtacc aagtgtggct gaatatcatg gataacgcca
    12541 taaagtacaa tcaaatcaat ggacagattc atatcaaact atttgagaca gccacagaga
    12601 ttgtactgga agtgaccgat tcaggtgtag gaatgaatga agagaccaga gatcgaatgt
    12661 ttgaaaaatt ttatcaagga gatacaagtc gtcaaatttc tgggaatggg ctaggacttt
    12721 cactggttaa aaaaattcta gaactgcatg atggacgtat tgattatagc agtatcgaag
    12781 gagtaggaac gacagcaatg atacggttaa gtaaacaata ggaaaaagaa taaaacccct
    12841 aaaaattcta ttgaaggttt ttaggggttt ttagtcgttt ttttgtgatt agggaaatgt
    12901 tcttacggtt cttttggcat aaaaatcgct gctttcttat tgaactacga gaaatgttag
    12961 aggtgtcatt gaaacaagga catgatgcaa gaaagagagc gaaagtcatg cgtcgctctc
    13021 tttttttggg tggatagatg gacagaaaat gtagggctta gttttcttct tgatttaaat
    13081 actcccaaaa tgtatctgaa gccaaagaat gctcaccgac tttacgagta aatgcaagat
    13141 aactctctgt tgttggagaa atagggagaa tcacagtatt ttcttttaat agattcttga
    13201 ctgttttttc cataacaata gcaatcccca tgtcatttgc aatcatattg atgatcaagt
    13261 cgattcttgc tcctttataa gagatttttg gttcaaatcc agcttcgcga cacagatcta
    13321 gtacaggttg atacaagttc gtagtggggc caaggagcaa aaagttctcc tttttcagtt
    13381 cgcttagatc caatttcttt ttactagcta aaggatgatt tttaggtaga actgctacaa
    13441 aatcgtctgt ttcagtaggc aaccactcaa tcgaacttgg tggggcctgg aaacttctag
    13501 caaagtggat cgtgtcacta tgttcattgg cgtcattgta agttaattcg acactttcca
    13561 cttctttcaa ctggactgta aattcaggat ggcgtttcaa aaactcggtg attttggaaa
    13621 aacctttata attgatcatt gtcggtatcg ttaagatatt taatgaaaga ttgttgtctt
    13681 tcgtataggt ttcaagcact tcttgcatag catggtattg ttctacgaca ttttttacat
    13741 atgggagcaa taagcttcca gcttcggtca attcgatttt tctatgggcg cgttcgaata
    13801 atttgacgtc taattctgtt tctaacgaaa gaatctgttt agaaatattt ccttgggtgg
    13861 tgaattgtcg atcagcagtt ttggtgaaat tcattgtttc ggctaaatca agaaaaactt
    13921 cgaatttatg gatatccatc atattcctcc tacttattcc aataaagaat actttaacac
    13981 aaaaaatgaa ttggtgtaat cttctctgtc tgttacaatc tttcttgcag cgaaagcaat
    14041 aattacagaa atttttctga tttttttcgc ttcgtagaaa aagagaggag ttttttgatg
    14101 ccaactggag ttttgatcaa tgtaggttcc gttttgtttg gcggactaat tggcggatta
    14161 gtaggaaata aattaagtga acattttaaa gcacagctga cgatggtttt tggtgtgtgt
    14221 tcgatgggga tgggaatcta ttccattgcg ccgatgaaaa atatgccggc tgttattttt
    14281 gctttagtga taggaacagc gattggtttg attgttcatt taggtaatgg gataaataag
    14341 ggggctgcgc tgatgcaagt acctatttct aaaatcttcc ctagtgaaaa gcttggtatg
    14401 acccacgatg aatttatcag cacattagtg acggtcattg ttctattttg tgcaagtggt
    14461 acggggatct atggaagttt agattctggg atgacaggag acagtacgat tttgatttct
    14521 aaatctgtcc ttgatttttt cacagcagct atttttgcgt gtaatttagg gtatgtcgtt
    14581 tctgtgatag caattcccca attcattatt ttttatattt tgttcttatt ggcaaaattt
    14641 atttatccat tgactacacc agacatgatc ttagatttta aagcttgcgg cggattcttg
    14701 atggtcgcta ccggttttag aatgatcaac gtgaaaatgt tcccagtagc tgacatgata
    14761 cctgcaatga tcgttatcat gccactgagc tggatgtgga caaattggat tatgccactg
    14821 ttataataaa aatcaaagag aagccatttt ttgctgacta aaaaggatca aatcttttag
    14881 tcagtttttt tgtatttgac aaactgattt tgaaactata ttatatgttg tataatatag
    14941 attgaaataa aatattacac ttgagaatag aaggtgacgt aatgcacatc cagattccaa
    15001 ctgttcttct tgatggtact gtgctagcag cattaagcaa ggaagatatg tatgggtatg
    15061 ccttaacaaa aatcgtacaa gaaagtctct ctgtcagtga atctacacta tatccggttt
    15121 tgagaagatt gaaaaaagaa ggatatcttg agacctatga tgaacctttt gaaggcagaa
    15181 accgtcgata ttactcgttg acaccaatgg gaagtcgtca tttagaagaa attattgatg
    15241 aatggattgt ttttcgagat tctttagacc ggttattgga ggagagcaca aatgaatgaa
    15301 tatttgaatg aagttgtgga tcatcttcaa gaaattcctg aagacgatcg ttttgatttg
    15361 atccagtatt atgaggaata ttttttagat tctggaaaaa cattagatca gatcattgat
    15421 gaatatggta caccaaagca gtttgcctta aaattaaaaa tcagttactt ttcagaagaa
    15481 gaacaagcag aaaacgagtt agtgaaaaat ccaaaaagac aaattcgcct gatttggtta
    15541 atcattatcg gtttatttgc ttctcctgtc ctcattccac tagcactggg gtttatattg
    15601 gttattgggg gactattggt tgctttacta gcgctcattt ttagtatcta tgttttatgt
    15661 atcagtatct taggtgttgg cctattcatg atcatctcag gaatcagtgt gtttggacaa
    15721 tcgattctga gtggattatt ctttatcggt gtcggtctgt tcgccacagg aagcgcagta
    15781 tttatttcgc cactgttgct gaaaggaaca aaagggcttt tttatgtcat gatgaacttt
    15841 gttaaatgga tgggacgacg ttttattaca aaaagagatc tgcattcagc gggcgtttga
    15901 aggaggacac ataaattgag attgaaacat tttttgatta ccggtattgg tctgatgctt
    15961 actggtggcc tcctagcagg tgcaacttat acattaggcg ctcaaaagag tctgacttgg
    16021 gataatggtc ctcggttgat cgatatggtt actgagacaa aaaaaatcaa ggatgcaaac
    16081 aaaatcgtca tccatgcaga aaatcaaaca gtttcggtca tacgcggatt ggattttgag
    16141 ataaaaacga cttatgataa gacagaaaag ccagaaataa aggagaaaaa tggcgtgttg
    16201 actgtgaatg cagtcaaaaa atcaacaggt gcaatcatta gtttagaaga cacaagtgca
    16261 caaatcacta ttgttgtccc tgcagatcat gaattatctg aattgacaat cgaagggaat
    16321 aatagtgcaa ttgatatcaa cgaagtatca atcaaaaaag tgaatactaa gacacatgat
    16381 ggctgggtat cgttcaatga atttacagga ggaacaataa ctgcgaaaga tgaattaggc
    16441 tcacttcgat tagatgaggc caatgtggat aacttgatcg tcacaggtga aaacagcaac
    16501 ttccatctag aagacagttt gatcagcaaa gaaggaaaag tcatcctaaa cggtggcaat
    16561 ttatctttgc atgagatgga aaacagtggc tatactatcc aaacgtctgg tgaatcttat
    16621 attgaaaaag actaccagcc aataacaaat actttgttag aacaaggaca gaaaaagcta
    16681 aagatcactg ctaaagacac atcgatcaat attacgacgg atcaagagga caatgaaaca
    16741 gattgatcct gtactcaact ggatatttta agaaaaaaca gcaaagatcc ttccaatctt
    16801 tgctgttttt catcgcttaa cgcacataac tatagtaaaa taagaaagaa aatatctaaa
    16861 aagaaaacag ggtgaaagag tatatgaagg tagaagaagg tcaaagagaa aagttaaaaa
    16921 cagaaatgga aagattacat acttatatca ctcaactaag ccaaaccttc tatgatccag
    16981 acaaagaaaa agtgatggtt aattacccga acaattcaga aggtcgccaa ttagagcaag
    17041 tgtaccatga agttttcaaa catttattga cggtgaagaa agagctggat tattattctt
    17101 taccgattat cgatacagga atattgaagt atgatgggaa gaaggaacga tttatcttta
    17161 aatctgttcg ggaagatttg cctctttcag cagggatgga tctagaggtc ttggtagaag
    17221 actattttac agaagaaaag cactgggttc gaacaaaatt ggattattta ccgcaggcag
    17281 ccggagggac acaggcaagc ggttggtaca tcactgaaga caaagagtta gaactagaag
    17341 gtgtaatggc gcgtattcga aaaaaaaatt aaaaaagtaa cattagatat ttattgaagg
    17401 ataaaggagc gcaggaagta gcatgagaga aaaaagagca ttttatatca atggttatgc
    17461 agggattgtc ggattggtcg tattagcttt agttggattg tttttcttct atttaggtat
    17521 gtggcaagcg aaagtttggg cacttttttt aagtattttt ctttggttga tcacgttatt
    17581 gttgttaagt tcggcaactg tagtcagtcc taatcaagca aaagtaattt tgttttttgg
    17641 acaatattta ggtacgattc gagaaaatgg ctttttcctt acgatacctt tggcacaaaa
    17701 aatgactgtt tcgttgaaag ttcggaattt caatagttct gttttaaaag tcaatgattt
    17761 ggatggaaac cccatcgaaa tctcagcagt agtagtcttt aaagtcgttg atacagcaaa
    17821 ggcattattc gatgtagcgt attatcaaga ttttgtagaa atccaaagtg agacagccat
    17881 tcggcatatc gctagccaat acccgtatga tacattcaat gagaatgatt tgacgcttcg
    17941 aggaaacacg acggcagtgt ctgacgagtt gcaaaaagag ctgcaagagc gtttagcagt
    18001 agcaggtgtc gaagtaatcg aaacacgctt gaaccattta gcctatgcaa cagaaatcgc
    18061 tagtgccatg ctccaacgtc aacaagcaaa agccattttg tctgctcgac aaaccattgt
    18121 tgaaggagct gtcacaatca ctcaaatggc cttagagcaa atcgaagatg gacaagaaat
    18181 caatttcacc gatgatcgga aagtccaatt gatcaataat ttacttgtct caatcattac
    18241 agacaaagga acgcaacctg ttatcaatac aggcgacatg aaagaccgta agtgacatac
    18301 tttttgtaag ataggaagaa ggagtttagg acggattatt gtcttgaact ctttttttag
    18361 ttgttttaaa gaaaatgaca aaattgaaag aagaatgata gttgattcga aggcagaatt
    18421 gttcgctttt ttctataatc agtatataaa gtaagagaga aggggaaaaa gtgaaaaaac
    18481 ttctgatcgg tctacttgtt ttcatcgttg tggtacttgc aggggtgaaa atcgcagatt
    18541 acgtagttat gggtggagaa gaatactatg tacaaattac gacagatggt acaaaaacag
    18601 tttcaaaatc agactctgga caagaaacag tcaactatag atatatcctg cctggctata
    18661 cgaaagaggg taaggaaaaa gaactggact tcaagggaca attgactcga ccgcttcgta
    18721 aaggagcata tttaaaaatc acctggaaca aaaataaagg tgtcacttcg tatgaagaag
    18781 tcgaaaaaaa agacgtgccg aaaacagcac tggaaaaact aattaaaggg tagggaaaga
    18841 aatgaacaaa atagtcgaag tacagaatgt aacaaaagta tacggaaaaa acaatgagaa
    18901 aaagacgcaa gcattaagcg gcatcagctt cgatgtagaa aaaggcgaat ttattgggat
    18961 catgggcgct tctggttctg gtaagtcgac acttttgaat attttatcca cattagataa
    19021 accaacagat gggcatatcc gaatcaatca aaaagatgtg accacattaa aaggaaatca
    19081 actagcagat tttcgtgcga atgagattgg ctttattttc caagatttca atttattaga
    19141 aagcttgaca gcacaagaaa atatcgctgt tcctctttct ttacaaggcg tcagaccaaa
    19201 agaaatcaag caacgtgtcc aaaaaattgc agaacgttta tccatctctc atattttgga
    19261 aagctatcct tctgagattt ccggtggaca aaaacaacgg gtcgccgccg cacgtgcatt
    19321 gatcacacaa ccaacgattc tattagggga tgagcctaca ggtgcgctgg attccaaaag
    19381 tgcccgggat cttttagata cgatggacga attgaataca aaagatcatg tatcgattct
    19441 tcttgtgacg catgatccgt tttctgcaag ttattgccaa cgtattttat tcatcaagga
    19501 tggcggtatc catcaagaag tgaaacgtgg agatcaaagc cgtgattcct tctatcgaga
    19561 aatactatct attcttggaa acttggaaca gtagggggag attgactatg ctttggaaat
    19621 tatcacttac tgggatcaag agccgtttaa gagactactt ggtgttattc tcaggattag
    19681 ttatggcatc tgctattttt tacatgtttg aatcaatggc aagcaacgaa gcttttttga
    19741 aaagtaatac gatcgttgcg gctacagtta tcattttccg attcggttct gttttactag
    19801 ggatcatcac attcgtttat attttatatg caaattcctt tttgatgacg atgcggcaaa
    19861 aagactatgc aatgtttatg atgctgggag caaaaggcag aaaaatcgca cagatgattt
    19921 tttctgaaac attcttagta gggatcgcag ctactttagt gggttcagct gtcggtgtag
    19981 gattgacaag tatcgtccac cgattattag tggatcagtt agatctcaat atttcgcatt
    20041 ttacaccttt tagtattcac gggttgttga tcactgtttt gttttttgct gttttgtttt
    20101 tattagctgc tgttgtcaat gctttttcga ttgtcaaaaa accgatttta gcattgattc
    20161 gtgcggatca gacaccaaca agaatcaagc agcataaatt cttgtttttc cttgaagtag
    20221 tactgggaat tgcttttcta ggctgcggtt actatatgat gaaacacgtg gggacctatc
    20281 aagtctttgg tataggggta gccttagtta cgatcgtatt aggaacttat ttcattttcc
    20341 attctgtgat cattttcttt ttatcattac taaagaaatc agaacagatt tcattgaaaa
    20401 aattgaacaa tttcacattg tcacagttaa actttcgtat tcgtgaatat acacaaatgc
    20461 tctcaatggt cgctatgctg tttgccttgg cactgggcgc tttgacggta ggattaggtt
    20521 ttaaaaatga aattgcagac ttgacagaaa acaattctgg atatgatcta gtgatgaata
    20581 atcctcgggc agaagatcag caaaaaatca aagaactttc accaacactt tctgcttctt
    20641 atactcaaaa agaagatgcg ggaacgattt actataatgc aagtgaattt gcggctcaac
    20701 cattgatcat gataaaacat gaagatggaa caccgccgaa gatcacgaaa gaaaaagttc
    20761 ctgctaaaaa aatgaatgaa ctgactgtac aagaggaact tcgaagctat gaattgccgg
    20821 aacagcaaga aaaagaaatc aaggtagtca gtcaatcaga atttgatcaa ctgaacttgc
    20881 cacaatccac tttacagcta gttcaggtaa aagacttcca agcaaacctg aaaccaatcg
    20941 aaacacttgt tgctcaaaat aaaatgaaca atccatcttt gcaaaaagta gagtacagca
    21001 accagaaata tgaaatgtat gaagtgtata atggtctatt ctctggtttt gaatttatgg
    21061 gattcttcct aggtttagct ttcttgacca tgcttgctag ctgtttgatg ttcaagatct
    21121 tgtcaggaag caagagtgac attgctcgtt atgaaatgct tgaaaagata ggaacacgac
    21181 gcgggttgct taaacaatcg atccgtagag aagtaggcgt gttattctta gcaccaggaa
    21241 tcttaggtgt agtccatgtc ttattcggat tacaaatgtt tggtctattc atgcaaaatc
    21301 catacaaaga tatttgggtt ccgtttacga tattcttcgt attgtatttc atctactatg
    21361 tactgacgac atggttatat actggaatcg tattgaaaaa gagattgtga aaaagctgtc
    21421 ctgcatcaag taataagaac agccaagcaa aaatagctcc tcatattttt cgtttggttg
    21481 ggcttattac cgcagatgca agcttttgaa caccgtttgt tcggaaaaag aggttgtgac
    21541 aatatttttg tcacaacctt tttttcacgg tcttttatta gaatatagta aattttttct
    21601 cgttactcac ggaataagct gttaagaaaa cggtttccat gtatactgga atcagtgcaa
    21661 atgaaagaaa agagggtgca aggaacatgg aaatcaatga acaaaatttt ggcgaaaact
    21721 atcacttgat tacgatcacg aatcaaaatg ggacatcttt gtctgtcagt gatcttgggg
    21781 cacgggttgt tcattttcaa acaataatcg ataatgaaaa acgagagttg atcttaggtt
    21841 ttgatgaagc aaaagaatat ctagaaaaag acccttatat cggtgcttca atcggacgta
    21901 tggctggacg gattacgaat ggcacattca aattagatga tgaaacgttc gaagtaaaaa
    21961 cagacccgga cactggtcat tgtttacatg gtggtgcacc tagctttgaa gccaagcagt
    22021 ggcattatac agtcatcaat ggtgaaaacg aagcatcggt cattttctat cagactagcc
    22081 cagatatgga aaacggattt cctggaactt tagatgtaga agtacggtat acgctgacga
    22141 acgatgatac atggcgagtg acggtccaag ggatcagtga ccgcaaaaca gtattcaatc
    22201 ctacaaatca tgtctatttc aatttaaccg gtgatccagc acagtcaatc gacgaacata
    22261 tactttggtt gaatagccga ttatttgcta cgctcaataa agatacaacg cctacaggag
    22321 aagtagtttc agtaagtggc acagcatttg acttccaacg tcctaaaaaa ttaggtgagg
    22381 tctttgccag tgattttcgc caatcaatga tagtgggcgg catcgatcat ccatttttgt
    22441 tgaaaaaaca atctggttta ctaaatgctg cggaattgac aagcccagat gaaaaagtat
    22501 cgatccaagt aaaaacagat gcttcaggaa tcgtgatttt tacagcaaat tttggagaag
    22561 atggaccgga aatgcgcggg aataaattag taaatcatgg cggtatcaca tttgagactc
    22621 aagaactgcc aggagcggag cgacttgaaa gttttggaga tgtcactctg gaacctaacc
    22681 aagtacgcga gtttgtaacg gaatacaaaa taaatataaa gaaatagaat aaattaaaaa
    22741 gggagtgtga cagtgtcaca tccccttttt aatttattct atttctttaa aggcagatca
    22801 agccagaatg atttgtctaa acttaacaag gttagtacag gaacagttgc agctttaact
    22861 gtacgagtag ttgtttctgg tatctgtctt gccgtaaaat tgttccacaa atcccagatt
    22921 gaagcgacac ggaagatgga agaagcgttg ttgacgcgtt ttatcgttga aaattctaat
    22981 cctgtgattg gcacgccatc tgttgtccat ttgattggca catcgaatcg cttagacata
    23041 ttgaggattt cagcgtttgc ttcttcagtt gttgctgctt cttttttcgg ttcatcatat
    23101 tttgtcaggt cgtacgcttc gatgatcgcg ttcaattttt gtgaatattg gcgatctgta
    23161 gcataacgtc cttccaaata tttggttgct tctttgtagt cttttgtttc cgacttccaa
    23221 gttggtttgt agaaatcagg attgccagtc agtccatttt tgatcaagtc ggcatagtct
    23281 tccaaacttt ccttgtaaga aggatattta cggaattgtg cgcggatttg gtagctttgt
    23341 ccggaactat cttgttcaag tgtattgaag gtcacgctgc ttcctttata tgatcctttg
    23401 ataccaaaaa ggttatgatt tggttcgctt gataataagc tgtttccaga tccactttca
    23461 aggatagctt gagccatcat gactgatgcg tagattcctt ctttttgccc gatttcctgt
    23521 gcatcatctg caatcttttt caagaactga tcagttgttt ggttttgact gaaatggaac
    23581 gagccatcta atccatctaa aatagatgag ccgcctgtca gcgaacctga agcagcaggt
    23641 aaacctgcta agtttactcg gacagcaaaa tcaggcgtga aatctgaaat agaagttact
    23701 cgaactttat ctcctggttg aggcgcatgg ataaattgtc cgttcccaat atagatacct
    23761 acatggtgag taacaccgga agaaggtccc cagaagacga ggtcgccagg ttgggcctga
    23821 cttactggaa tctgcgtacc tgcatattgc tgttcgcctg tccagccacc gatataatga
    23881 ccggttgctt tcatatatac gtaataagtc aaaccagaac agtcaaaagt atttggacct
    23941 tttgctcccc atacatatgg ttttccaaga tgcttgaatg cttctcgaac gattgcttgt
    24001 tggacattcg ttgtatttgt aggcaataca gcatcaattg gtgattttac aacaaaatca
    24061 gaagaagcag aagatggatt ctctataccg ctttgatttg ctgtatttgg acgagatgaa
    24121 gcattgttcg ttccgccatt cgatgaacca ttgtttggat ggtttggttc tctctgagta
    24181 tttccattgt tattcgatcc agatgggttt gtttgattcg aagaattccc gctgttcgaa
    24241 ttattgttat tattgtttga attatttgaa ccattattat tgttgttatt acccacatct
    24301 ggtttgtttg gcgtttccgg atctggtgta ctagggactt cttcactagg atcagttggt
    24361 ttctctgttg aagtatccgg tccagcttta aattctgcag taatagataa attgttcgta
    24421 cctactgtaa aggtctttgc atcataattt acttctttgg caccattaac agcgactgaa
    24481 gcaatttgtt caccttcagc tggcgtaata gtgtaagtta ccttcgtttc cgctaataaa
    24541 ccttctaccg ttccactttc cgtaatatcc aaaggttgct gttcagggga atccgctgtc
    24601 gaagcagtca gttggatctt accaatcaaa gaaggagcaa ttgtaaggga atatgtctcg
    24661 gctggctttt cattttctga tggatcagcc ggatcaattt cagttggctc ggtcgatcct
    24721 gttgttgagt tagttgttcc agttgggtct tctgtaaaag aatccgttgt ttcactagta
    24781 gctgagctgg atgtagtcgt atctgttgtg actgtttgat cagctatttg attatctacg
    24841 gcaaatacgg tattggtcga caaaacagac ggagcaatca gccaagtagc agctagcaaa
    24901 cgtatcgttt ttttattttt cataataaac tagtcctttt cctttcattg attttctgtc
    24961 ttattcagca ttcattagac attttttgga acagataatc gtgtcgaaaa gctttttctt
    25021 ttcataaatc tgtctgtgta ttctaaataa aacaatttgt aaataagcaa cgagcattca
    25081 ttcgtttttc tttgacgttc attttattgc atagaccacc tttgtttggg gataaacaag
    25141 tggggataaa aatggttatg catatgtaaa ttataccaaa gtttggaaag tatcacaaaa
    25201 atttataaaa ataatgatcc taaaaagaaa aagactgaaa tatgtgataa acatctcata
    25261 cttcagtctt tttcattaac tattcataag acattaggtc ttcatcaaac ccgctaacga
    25321 gagtagtgat cattgctacg actagactaa cagccagtcc tgctaagtac cagaaaaaag
    25381 taccggaaat gaatgcaggc aatgtggtca gactaccaaa tacaaaagcg atcgctttca
    25441 cttgggcaaa tcccattaca gctccaccag cagctgcacc aatgcaagca gcaacaaatg
    25501 gttttttcaa tgggatcgca attccgtaaa cagttggttc ggtcactccc gcaagtaaaa
    25561 tcgaagcaaa agcagaaccg gagatcgaac gggtttttga acgtttactt cgcagaaata
    25621 cacctaaagc tgcaccagcc attccaaaat tggctgctcc tgtcaacgga acgattcggt
    25681 cgaacccata agcagagata ttactgatca tgacagggtt tacagcccat tggataccaa
    25741 atattaccaa aatattccaa ccaccagcga tgattgcgcc actgatcggt ccgttaatat
    25801 ccatcatcca agtaattgcg gcagctaaac cttcgccaag gtaaacaccc gcaggcccga
    25861 ataccattaa cgtcaaagga accgtgataa ctaatgaaag taaaggtaca aatactaatt
    25921 gcatattttc gcgaatatat tttttgagga atttttcaac aaaagaataa aagaaaatac
    25981 ctaaaatcgc cggcatgaca gtagacgtat actgcatcag tactacagga atccccagaa
    26041 aatcagtagc tgctttgcta ctgtctgaaa gtaaagaggt cagattcggt tcaatcagtg
    26101 ctgcagcaat gacagccgca atatatccat tcactttaaa cgttttggca gcagaaaaag
    26161 caagaaagat tggcagaaaa taaaagacgg cattcgacgc agcagacaaa atatgataag
    26221 ttccgcttgt ttcactaagc cagccaatcg tagtcgctaa aacaaccaga ccttttaata
    26281 taccagaacc ggcaaacagc ggaatgatcg gcgtaaagat ttccgatatc aaaccaaata
    26341 gtttttcgat cggatttagc ttttctttcg gagctgaatg atcgatttga cgcgtcaatt
    26401 ccttgtaagc aggagccaat ccctgatcca tgattatttc tgcatatcta tcgagtaaaa
    26461 tgaaatactt aatggaaggg atttgctgtt ttaatttttc tgcattggct actggctgtt
    26521 ttttcaattt gatacgaagc cgagtcgcac atggaaggac agaatcaata ttgtcttttc
    26581 ctcccaactg ttcaatcagc atatgaatct gctcggacag taccatctgt tcaggattca
    26641 ttgtcgtttc cattagaaga ccactccact cactaatata atattggcgt aaggtgtcat
    26701 ttcgcctgta cgaataaatg catgacagtt attcaactct tttttcattt cttcatgtgg
    26761 gataaagcta atcggtgtat ctggtaacaa taatttgatt ttttccaata tttccgggtt
    26821 cattgtcttt atttcttccg ccaattcaat cgattgtacc tcaagttctt ctaaaacgtt
    26881 ttctaaaaca gataaaaaac ttggaagacc gtttgagaca gccaaatcta ttttttcagt
    26941 accaaagggg acaggcatgc ctgcatcccc gatacttaat ttatcaaagt gccccatttg
    27001 tgcgattact cgtgaaatat ctgaattgat tacttttcct tttttcatgt ttgtttcttc
    27061 ctctcattgt taattttaga ctgctgattg ttctaaagct tcgcggtaag ggatagaagg
    27121 ttgcgcacca taagactgca cagcaacaga agaggctaga tttccataaa ggatcgcttc
    27181 tttcaagtta ctaaggtctg gctgaagtgt gctgcttaat gcgccaataa atgtatcacc
    27241 tgcggctgtt gtgtcaacgg catccacttt aaaagcaggg acgataccgt gttctttttc
    27301 tgtatgatag aaagcgccgg cactgcctag tgtaatgatt actgttccaa tacctaattc
    27361 atggagtttt tcggctgctg cgaccaaact gttatgatct gtcactctga ttcctgtaat
    27421 gatttctgtt tctgtttcat ttggtataat gatatctgtc ttttcaagta gttcagtagg
    27481 aatcgtttca cgagctggtg caggattcag gatcgttgtt tttcctgcgt cttttgcaat
    27541 agaaaaagct aggatcgtcg catctaagct actttcaaac tgagcaataa taaagtcact
    27601 gtttgtaatc agttgcttgt tttttaggac ttcttgttca tggaaagcat tatttgcccc
    27661 tgcatggatc aagatactat tttctcctgc tgcgtctacc ataatcattg cttgtccggt
    27721 tgtggcgcct tggatttcag ctatcccaga tgtatcgatg ttttcttttg tcagtagatc
    27781 taataactgt tgtccttcgc tgtcagcccc aacgcctcca ataaaagatg tccgtgcgcc
    27841 tgaacgtttg gctgccactg cttggttggc acctttacct ccgccagcgt gaaaaatctc
    27901 atgcgcatgc atcgtttcgc ctggcttggg catgttggtt aaacgaaggg tagtgtccat
    27961 attaatactt ccaattactg taaccgtgtt cattttacaa acctccaact atctcattta
    28021 ttttgatggg aaaattattt tttaaaacgt tttagtaaaa cgttttaata gttcgataaa
    28081 aatatagcac accttgaaag ctatttcaag ggtgcagttg aaaatctttt ttctaatcgt
    28141 acaggtaatc ttttttcttc ccattctttt tctgggaatt ggatccgatc taacaataac
    28201 ttggcagatg tttgtcccaa ttcgaagatt ggttgtgcga tcgttgtcag tttcggcttg
    28261 atatactcgc acatatctat attatcatag ccaataatgc tgtagtcttc agggatgctt
    28321 tttccagctt cctctaatcc gcgatataat ccaaaagcta attcatcatt taaagcaaac
    28381 acaccagttg cagcagaatc aagaagttct gctgtgatct ggtagccacc ttgttttgag
    28441 aactgtgtag gtaagaggat cagttggtcg tgagggattt ggtaaacatc aagagcagat
    28501 ttaaacccct caatccgagc gtggacattt tccggtggat tttcaggata gacaagggcg
    28561 atcgtttgat gtcctagact aagcaagtgc atgcccgcta ggtaaccacc gcgaaaatcg
    28621 tctgttcgta cggcatcact aaatccttca gattttttct gatccaaaac aatgaagggc
    28681 cggccttgtt ttttcaaatt ttcgttgatt gcatctgtag agacagcaga agtcgcgata
    28741 atgaatccat ccacacctcg acgtgtgagc tcggctagat attcgatttc tttttgatga
    28801 tcagaatcag cattacataa tatcgtgacg aaattctgct tatacaagat atcctcaatt
    28861 cctcgcatca gtgtactgaa gaatggattc gtgatatcag gaacgagtac accaattgtt
    28921 ttcgtttcct tggtaatcat ttgtctagca aaatagtcag gctgatagcc taattcttct
    28981 ttggcagcta aaactttctg aacagttttc ggactgaatt tctccgtatt tccatttaat
    29041 atcaaggaga cagttgtgat tgaaacgccg gaaagtttag cgacttcttt gattgtgatc
    29101 ttttttttca ccatgttcat actcctatct aaatcctgtg gttattttcc catgaaatca
    29161 aaaagaagta aatgaaaatc acgtgtgcca gtacttattt gctttgttgg atagcttcga
    29221 ttatttgttt cgttgagcgt actcgtgcag ttttcggaaa tatctttttt atgaacaatt
    29281 gatgggattc tttgtcacga tcactgcaag catcttcagc aagcacgata tggtacccgt
    29341 gttgaaaggc atctaatgca gtagcatata caccgtttgt tgtcgctaca ccgcttaaaa
    29401 tgatagtttc gattccacgc cggcgaagtt gaagatcaag agaagtacca aaaaaagcac
    29461 ttggattgta ttttgtgata gtcaacatat tatctgtatc tttcaaagaa tccttcaaaa
    29521 gtaaatcagt gaattctgga ggtacagaag cagtcattcc acccatatca gtttgttgac
    29581 tcaaataagg gaaagtggag atgtctacgt tcactaaaac tttcaaggca gcggtacgtt
    29641 tcaatgttct tactaacaaa tcattgtttg tcagaataga tgctgcagtg tgtggagaga
    29701 gtgtaccgtt atttacgatc tctttttgca tgtcgatcgc gacaaaagca gtttttgata
    29761 aatcaaaaat catattttcc tttaaatcat tcatgatgtt ccttctttca aataacaatg
    29821 ttttttatgt aagtaaatca taaatcgttt atttgtcaac taaattaaag gattcctttc
    29881 tttagtctag agaaattcag tggtaaaata aataagtgtg agaaaacgca ttcgatgcat
    29941 agatatagcg agaaatatcg tgaaaatgga ggaatcagag tgaagtatgt cgaagtaaaa
    30001 aatgaataca aacgatacaa aatgggagaa acgaccatta cagcaaatga cgatatttct
    30061 ttttcaattg aaaaaggcga attagtgatt atacttggtc caagtggagc aggaaagtca
    30121 acgattctta atattttagg tgggatggat acacctgatg aaggtcagat cattattgat
    30181 gacacagata ttgcgcaatt ttcagataaa cagcttactg cttaccgacg aacagatgta
    30241 gggttcgttt tccaattcta caatctcgtt cctaatctaa cagcaaaaga aaatgtggaa
    30301 ttggcaacgg aagtttctcc tgatgcgctt gatcctgtag aggtattgag gcaagtgggc
    30361 ctagctcatc gattgaataa ctttccgtcc caattatcag gaggcgaaca gcagagggtt
    30421 tcaattgctc gtgcactagc taaaaatccc aaacttcttt tatgtgatga accgacaggc
    30481 gcgctggatt ttgaaacggg caagcaagta ttgaaattat tgcaaaacgc tagcagacaa
    30541 cacggcaata ccgttttgat cattacacat aattctgctt tagcgcccat cgctgataga
    30601 gtcattcaca ttaatgatgc taaagtaagg tctgttgaat taaatgatca tccttcatcc
    30661 attgatgaga ttgtatggta gggggatttt atgaaaacaa gaacatatct taaagcaggt
    30721 tttcgcgaaa ttcggcaatc taaaggacga ttcattgcaa tcatccttat tgtcatgcta
    30781 ggaacacttt tatttgtagg tgtcaaaact gctggacctg tcatgcaaaa aacgatggat
    30841 cactatgttg gtacagcagg actttctgat ttgcaaatcg tttctactgg tggcttgaca
    30901 caaaaagata ttgctcaagc agaaaagatc ccagatgcaa aagtagaaac aggaaaacag
    30961 atctattatt ccaatactgg caaaaacgaa gtgattcaaa tattttctta taacaaaaat
    31021 aacaagcaga acaagctaca attaactgat ggaaaactcc cggaaaaacc aaaccagctt
    31081 gtattggatg aaaaagcgga agatgaaggg tataaaatcg gtgatattta tcagattgat
    31141 tcagatgaat tgaaagaaaa agagtatacc atcacgggct ttgtccgttc tccattgttc
    31201 atcaacaatt tggagagagg atacgcgaac gtcggaaatg gtagcgtcga ttattttgtc
    31261 tatttacctg aatccgtttt taaaacagat atttattctg tcttatatct tgattttaca
    31321 aacgtaaaga atttgaatac gtacagcaaa gcttacaaag ataaaatgga gcaaaatcaa
    31381 gaaaaggcag aaaagtattt acaagatcga cccgaagaac gcttagaaga gctgaaaaaa
    31441 tcggccaacg aagaattaga accggcacag caaaaaatag cggatggcaa agcacaaaca
    31501 acgcaagcaa gaacacggtt agaagatgct aaaaaacagt tagcgcaaca gtcagcaact
    31561 atccagcagc ttcctgaagc gcaacgaaca atcgctgttc aaacattatc tcaacaagaa
    31621 gctcaacttg ctgaacaaga aaggcaatta gttgagaaag aacgtgaact ggcctctgca
    31681 gaggaagaat tgaatcgaaa tgcagaaaaa attgaaaatt taactaaacc atcataccta
    31741 tacaatgaac gttcagaaaa tgtcggtttt caagaatttg gtgatctgtc tgatcgtatc
    31801 gctgcgattg ccaatgtttt tcctgttttc tttttcttta ttgccgcatt gatcaccttt
    31861 acgacaatga cacgtatggt agaagaaaac cgcagggaaa tcgggacatt aaaggcgtta
    31921 ggttatacga aagttgaaat cgcaaaaaaa tatgcaatct atgcaagtct agcctcaggg
    31981 ataggtattg ttttaggaac gatattagga acgaatcttt tgccaaggat catttttgag
    32041 ctgtctaacg agcggtatga tattgggagt gcgcttattt tttatgactg gccgccgatt
    32101 ttacaagcag ctgccgcatt cttcattgcc gcatttggcg ctgcaatgat cgttttattc
    32161 aaagatttaa gagaacggcc tgctgcattg cttcaaccga aagcaccgaa gccagggaaa
    32221 cgcatttttc ttgaatacat cactccgctg tggtcacgcc ttagctttaa tcaaaaaatc
    32281 agttaccgaa atctcttccg atataagtca agaatgctta tggcgattat cggaattgct
    32341 ggatgtgccg ggctgatggt tgcaggcgtg ggattgaaag attctctaag ttctgtttca
    32401 gccaaacaat ttggaccgat cattgactat caagcaatcg ttacattgga ttcatccacg
    32461 gaagatagtc gtcaaaaagc agcggatatg ctgcagaagc aagaaaaagt aaatgagatc
    32521 ctagctattc atactcaaac cgtagaattg agaaaaaaag gacatgcttc gcaaagtctg
    32581 acagtgatcg taccaagtaa aacagctgat ttggatcgtt ttatccattt aactgatctt
    32641 gaagagaacc cacgctcctt gcctaaaaca ggaatcgcta ttacgcaaaa agtagcagaa
    32701 ctatttgact tatctacagg agatcatatt tcgctttatg atgaagatca aaaagaatgg
    32761 aaagtcgaag taaaagaaat cgtgaaaaac tatctaggta gctttattta tatgtcccct
    32821 gaatattatg cttcagttac tgatcaatcg atgatggata atgcattttt gctgcgtaca
    32881 gataagatga caacaaaaga agaagaaatg ctttccgaga aactgcttgc cacagacgct
    32941 gtgacaaata cgtctttcat gtccaaacaa atcgaaacgc aagaagaatc aatggcgaac
    33001 ttagactcgg ttgtcttaat ctttgtcgta ttatcaggac tgctggcttt tatcgtcttg
    33061 tataatctaa ctaatatcaa tatttccgag cgtgtacgcg aattatctac aataaaagtc
    33121 cttggatttt ttgataaaga agtcacgatg tacattgttc gagaaaatat catctttact
    33181 cttttaggaa tcatcggagg atttggtgta ggatatatat tgactgactt catcttgcaa
    33241 caagcgtcta tggagaatgt catctttcca ttagtgatca catggtgggc ttatgtctta
    33301 tcagcaggat taactatcgt atttacggtt attgtgatga ttgttaccca tttcaaatta
    33361 aagcatatcg atatgattga tgcgttgaaa tcgaatgaat aaaaacagag gttgtgaaat
    33421 cagcggtttg acctgagtat tagagaaaca aacggcaaaa tagtttttca tattttgccg
    33481 ttttttgagc taataccgaa ggacagctga ttgaacacca tgaatcagag gttgtaaaaa
    33541 agccgtcctg catcgagcaa aaagaatact caaataaaat tagctcctca tgttttacat
    33601 ttgagaagac tgattgccgt agataaaagc ttttgaacac catttataga agaaacaaga
    33661 atattttgct attttaaaca agaggataga caaatacatg tctgatctct tgttttttat
    33721 tgggcaacag acctcttaga atgtataaag ataaatttta caaacgcttt cggttcagtt
    33781 ataataataa atgataactt gtatatataa gttgtggtag gtaataaatc agttgaatgg
    33841 aggtaaatat ccatgacatt taaagacaaa gtcatttacc aaatctatcc caaatcttac
    33901 aaagacagta atggtgatgg aattggagat ttaaaaggaa tcaaagaaaa actcccgtat
    33961 ctgaatgatc taggcatcga catgatttgg ctaaacccta tttatccaag ccctcaaaaa
    34021 gacaacggtt atgatatctc tgattacaca gccatcgatc ctatctttgg tacgatggag
    34081 gaattcgaac agctaatagc cgaagcaaag aaataccgga tagaaatcat gctcgatatg
    34141 gtgctgaatc acgtttcgac agaacatgaa tggttcaaaa aagcactagc aggggaccag
    34201 cattatcagg acttcttcat tttaagagaa gagccaacgg attgggtatc aaaatttggt
    34261 ggaagtgctt ggtcgccatt tggagatacg gggaaatatt atttgcactt atatgatcgg
    34321 acacaagctg acttgaactg gcggaaccaa gcagtaagag aggaactatt caaagttgtt
    34381 cgtttttgga tggaaaaagg ggtaaaagga ttccgtttcg atgttatcaa tgttatcggt
    34441 aaagacgaac aattgaaaaa taataaagaa aatgatggca aacctgaata tacggacaaa
    34501 ccaatcaccc atacgtatct aaaagaattg aaccaagcaa cgtttgggac ggattccact
    34561 atcatcacag tcggtgaaat gagctctacg acgatcgaaa attgtatctt gtatacagaa
    34621 cctagtagag aagaattatc aatggtcttt aatttccatc atttgaaagt cgattatgaa
    34681 aatgggaata aatggtctac tcctccattt gattttgcag aattaaagcg actatttcat
    34741 acatggggag aaaagatgag tgagggtaac ggctggaatg ctctattttt taataatcat
    34801 gatcagccac gtgcgctcaa tcgatttgta gctatcgatc agtatcgaaa acaaggagca
    34861 gagatgctag caaccatgat ccatttgaac cgtgggacgc cctatattta tatgggagaa
    34921 gaaatcggca tgatcgaccc agactattca tcgatttccg actatattga cgtagaaagt
    34981 ttgaatgctt ataagttact ccttgaacaa ggattcacag aaaaagaagc gtttcgtcgg
    35041 atcaaagcaa aatctcgcga taactctcga acacccatgc aatggtcaga cacagaacaa
    35101 gcaggattta ctacagggaa accttggtta aaagttggag gcaagctaga agagattaat
    35161 gtagaaaaag aactatcttc tgaagattct attttgtcct attacaagaa attgattcgg
    35221 ctgagaaaaa catatccaat cgttgcacaa ggagattatc atgcttatga agcaaatcat
    35281 ccggaagttt atggctttct tcgccaattt gaaggacagc agctgcttgt attaacaaat
    35341 ttctatgata aagatacaag gatcgccatc ccagaaggat tcacagaagc caatattctt
    35401 atcgataatt attccgatac tgttcttcat caaagtctgg agctaaaacc ataccaggca
    35461 attgctttgc tgaaaaatta aaacaaaaac acaaaacgtc gttcctacga gctattctcg
    35521 taagatcgac gttttgtgtt ttatttaaaa taatcttctt cagaaaatga agagttttca
    35581 tactctcctt tatcgccata aaatttattg tagcgttgtt tgaatttacg gaaaataaaa
    35641 gtaacgagta atttgactaa cgtatagata ggaataccaa aaataacgcc cacaattccc
    35701 attagatcac ccatgacaag taaaacgatt aagatcgtaa tcggatggat ctgtaaacga
    35761 tcacccatta ctcgaggaat caccaagtca ccgtggagaa gttgtacggc aaaccagaca
    35821 atgacaaatt tgacagccat aaaagtagag tcttggaatg caatgatcaa acctggaaag
    35881 aaggcgataa acggcccaat atatggaatg atacataaca acccagcaga aatagctaaa
    35941 acactagcaa attctaatcc gatcagtaag aaacaaaccc agtagactgc tccaaggata
    36001 aatgaagcaa tgatttgccc ctttagaaat gaacccaatt gttgattagc aatctttgtc
    36061 aaatttttaa aatctgtacg aaatgctggt gggacaatcg ctaaaactga ctgataaaat
    36121 ttttgaggat ctctcaaaag gaaaaaagcg acaattggac cagtaaataa agtaataaaa
    36181 atagctgtaa cagttgaaaa aatgttgctg atccgttgtg cgccactttc ccaataattt
    36241 ccaataaatc cagctatatc agacgtcacg tcgtcaatag aagcgaaaaa ttgattaaat
    36301 gaatcggcaa aaggtgtttt ctccaaaaat tgtcggagac tctcttgaaa atcttgaaac
    36361 aaatctggca aactactgat caatgtcttt gcctgttctt caagaaaagg aataagccat
    36421 atgcttccaa gccccaataa aaataaaata atcacataaa cgataatgac tgacaagttc
    36481 ctcgaaaggt gcttgctcaa acgtttgatg actggttcca aaagataata caagatcagt
    36541 gcaaacaata atgggggcaa gatagtagcg ataatgattc cgacaggctt gaatataaac
    36601 gagatctgat ggaaaaaata aatgatcagc gcaagtaaga taactattcc taaaacatag
    36661 taagaggttt ttcctcctaa aaagcggatc aaacgattcg ttgtatctgt ttcttttttc
    36721 actctttttc ctcctttatc tataaattca ttatagacaa aagcgggtat aaaaggaaaa
    36781 tgatagaggt tgattgcaaa aatatcgtca atcattcata taaactttct catcaattaa
    36841 aataagaaaa cgcttgacag ttcttttaac catatgataa attaaatcct gttgctaaaa
    36901 cgtaactatt acggtttgcg aaaagcgaat cggaacattc aaatgaaaga agatgagaag
    36961 aggagtgtca taaatgaaac gattgatagg tatattggcc atgctggttt tagcaggtat
    37021 gttaactgct tgcggggcaa gtgggaaagc cgaggacagt aaagaaaaat tgagcgtgat
    37081 gacgaccttt tatcccatgt atgattttac aaaagcaatc gtcggggatg aaggagaagt
    37141 agagttattg attccagcag ggactgattc ccacgattat gaaccttctg ctaaagacat
    37201 ggcaaagatc caagatacag atatctttgt ttataatgat gaaaacatgg aaacatgggt
    37261 ccccgcaatc caaaaaacat tacaagaagg caatgtccat acgataaaag cgacagaagg
    37321 tatgctttta cttccgggaa gtgaagaagg acatgatcat gaccatgaac acggggaaga
    37381 aggccataca catgagctag acccacatgt atggctagct ccaagtttag caatcaagca
    37441 ggtagcaaac atacgtgatc agctgattga agcttaccca gaaaaacagg aagtatggac
    37501 aaaaaatgca gcagcctata ccgagaaact tcaagcactc catcagcttt atcaagaaac
    37561 ctttaagcaa gcaaaacaaa gatcttttgt cacgcaacat gctgcattca actatctagc
    37621 gttagaatat ggattgaatc aagtatcgat tgctggtctt tcttctagtg aagaaccgtc
    37681 tgcggcgaga attgctgaac tgaaacactt tgttaaagaa catggaatca actatattta
    37741 ttttgaagaa aacgccaaag atagtattgc cagaacgtta gcaaacgaag caggtgtatc
    37801 tttagaagta ttgaatcctt tggaaggatt gacgaatgaa caaatagaaa atggagaaaa
    37861 ctatctttct atcatggaag caaacctcga agcgttgaaa aaaacgacag agacagaaaa
    37921 cccgctagaa gatactttga cccccaaaaa agaaaagagc gtctacaacg gttattttga
    37981 agacgaggat gtacaagatc gctctctttc agattgggca ggaacttggc aaacagtaga
    38041 tacgtatgta gaagatggga cttttgatcc tgtttttgaa tataaagaaa aattgaatca
    38101 ggataagaca gcacaagaat ataaagaata ttatacgaag ggctatcaaa cagatattgc
    38161 ttcaatcaag atcagtgaga aagagatggc atttacgttt aagaatggaa caacgaaacg
    38221 ttcaaattat cgttatgtag ggaaagaaat cttgacctac gaggcaggaa accgtggcgt
    38281 aaggtatcta ttcgaagcag aagataagga aagtggcgct tttagatata ttcaattcag
    38341 tgatcacacg attgcaccgg ctgactccga acattttcat ttatatgcag gaaatgaaag
    38401 tcaagaagta ttgctgaaag aaatggagaa ttggccaaca ttttatccag atacaatgga
    38461 tggacgtgaa attgcacagg aaatgctttt gcactgatag gcaaaaaaga accgaagggt
    38521 ttggatcggc tggtcattta gtcggtccaa actattcggt tctttttatg aaatcaattt
    38581 ctttaatcca cggatagagg ctgggatggt ctcgccgttt gccaacgtga cttctctggt
    38641 ttttttatta atcgaccgca cattttgtgt attgataaca taggatttat gacaacggat
    38701 aaaattccga tgcattttct cgatttcttt gatcttctca tagaattgta attgggcatg
    38761 agtcaaatgg acttccagtt tatgtggcgc tcctagcgaa gtagaaaaat attggatgtc
    38821 tttgatcggt aaaaatttga tctcatcatt aaactctatt ttgaagatat ctttttggat
    38881 ttgggacatt tgaaaatctt cgaaaatttt gctcaggata tgctcgacat tttcttttaa
    38941 atcataaaga tcttctttga cgatataatc agtcggttcg atgttgcttt ttaagatttt
    39001 catcgacatt tccgtatggg aagtcacata gattattttt cctaaaggat ctaattcacg
    39061 gatcaattta ccgatcgcta caccatccat ttcactgtct tctagttcga tatctaaaaa
    39121 gtaaatacca aaagaagaag ttctgatctt ggctgcattg agaagttctt ccggatcaga
    39181 ggtagaaata tggacataac tgtctaagtt ttcaatcatg attctatttt tgatcaccgt
    39241 tgataaaact tctaattgtt ttggatcgtc ttcacaaata taaatgggaa tcatatcatt
    39301 actcctttat tttatttgta tataaatcgt ttgagaaaaa cgctggtttt caattgatgt
    39361 ttcaaggaaa agataaggat tggtctttgt caattcatcc aagataaaca agccaaggcc
    39421 ttcgtttttt ggatttgttt ttgttgagaa ccctttttgt ttcaactggt ttaacggtaa
    39481 tgtatttgtt gttgtattga tcacacgaat catcacggaa tgattcttct tgagtatggt
    39541 caattcgatt tttttcttct ttgcagcact agctccttca atcgcattgt ccaagataat
    39601 accaagcata cgtacaaggt ttaccgtatg tttcgtatcc agttcgattg gctcaggaaa
    39661 atgaagctgg acgatcaacc ctttttcctg tgccatcatg agttttaatg acagtaaact
    39721 acggatctca ggaactttca tattatccaa catacttaga ttggccggaa aaaggttcat
    39781 catctggcgt gtaggtaata tcgtttgatc ataataagac cgtaatcctt ctaagtcgcc
    39841 ttctgaaata tagctgtgaa gagaaaaaag cagattttgg taatcatgtt tgaattctct
    39901 caagtcttga tagttttttt ctagatcttt gatataatcc tgttgcatcc tgacttgttg
    39961 ctctaataac tgatcagcta atttcttatt agagattttc caataataaa ccaagatgat
    40021 cagggcaaca cacagatata gaatgaaaaa actcgtattc aaagctaaga catcaggtgt
    40081 ttcaccagaa aaacgggtat aaaaaataat gatatagtat gtaaataata tcaatgtccc
    40141 gatcgtaccg atcatacggc ggtccacagt gtgaaaccaa gtctctaacg atttttttaa
    40201 acaaaaagct aataaagcag ataaaaaagc ggataagaca atgtgaaaat actgcaatga
    40261 atctcctggt tcaataaccg aagatttcaa aacaaaaaaa tcgccgatgc ttgttaagtg
    40321 atctcctaaa agcgacgtca ataacccaaa aggaagcgcg taaaccaatt gttgacgcga
    40381 agtcgaaaag aagaaaaaag taagcatcag cattcctata gcaattagag cagtaaaaat
    40441 ccctaaaatc gaatagaccc atccaatcaa aatacaagaa atcagaatga ctcccgcaaa
    40501 cagatatttg tatcggatgt gatggtcaat caataagata caaattgtgt aaatcaaata
    40561 ttgcaacgct atggaccata aaaacataaa gtacagctcc cattgatttt taccaaatta
    40621 tagcacaatt ggcgtattat ttttttaatt tttcagggat tttgaattca gaactcatac
    40681 caaagcagca gccagataca aaaaagtcag ataagttagt taattgttta atgattgttt
    40741 ttttcatgaa agattcctcc aaaattcatc aagtagtttg taataggaag caacgtagct
    40801 atttctaaaa caatcccaag tagcaaatag gtagaaaagt aagaagctga gaaaaataac
    40861 gtaatgagag aaaaaattag aaacatgctg atcgccttct ttaataatgg gggacgtttc
    40921 tcgggttcaa tgggattgat tgctgtacct cttgatccat agcgaagcag taacaaaaag
    40981 cagctgatta acaaaaagac catcattggg aaggagatag tcaattgcag atgtttcatc
    41041 accaagggaa cgccccataa ataaagtagt gtccacagca gacaaacgaa ttcagagtcg
    41101 gaatgcaatc cataagcata tctgcggatg cagatataag aaagaaaggt gattcctgtt
    41161 tccgcccatg agcctgtcac cagagaaaag actagaacga ccgctagttt tccaagatta
    41221 ttcaataaaa cttctaagac aaattttact tggacataca ccatatcttc ttccgatttg
    41281 tctccgctga ataaggcttc taataatttg ttcacaactt tatctttcat ctcatacgta
    41341 cctccaccac aaattttatg ctaaaaaaaa taaaaaacgt cttttgtttc ataaaaagtg
    41401 gttttgacat caaaaccgtc atgatctcct aaaaaaatct tcgttttcca agaaccttac
    41461 caaaaaaagt gacagttttg atacgaaaag tactttttat gacaagtttg tgctttttcg
    41521 agtgatttat gttaaaaaat tactgacctt ataaaaggtg atggagggat tcttatggaa
    41581 aatgaacaat tgtttgaagt cggcgaaaca tatgcattta aatctgattt atttgaatca
    41641 ccaataaaag gaaaaataga aaaaatctat gaaaatagtg tgatgatacg tgtcatgaaa
    41701 tgcgcagtag aagacaatga taccgctcat aatctgcact ggcagttagt tgtacgcaaa
    41761 tcaagtacaa tagaagaata aaaacagata aaatgttgag agcataaaga ggttgtgagt
    41821 ctcccgttta gctctgagtt acaaggaata atgttaaaaa acagcttttc atgttttatg
    41881 aatattgcct tgtgaccgag gtgttggcag gagaatactg tttatttgaa aaaagggctg
    41941 cgacaagact tttgtcacag cctttatttt catgaatagg agttactttt ggctactttt
    42001 agagaagtag cttttaagga gcagataaag tcaaggcttt tatttctata aatgaagtag
    42061 taaaatgaag aacagatttt attacaggaa ggatgccgat aattgtgatc attgttgcag
    42121 gaacgatcgg tgcagggaaa agcacactaa ctgaaatgct tgcccaagat ttagaaacaa
    42181 aaccttttta tgagaacgtg gaagataatg aagtattgcc gctattttat tctaatccag
    42241 aaaagtatac ttttttatta cagattttct ttttgaacaa acgctttttg gcaatcaaag
    42301 atgctttcag ccatgatgac aacgtactag accgctccat ctacgaagat agtatgctgt
    42361 ttcatttgaa tgcagattta gggcgtgttt ctgaagtaga agtaaagcag tatgaaggtc
    42421 ttttagaaac gatgctgaag gagttagaag aaatttcgcc acaaaagaaa ccagatctac
    42481 tggtttatat tcgtgtatcc tttgaaacta tgttagcgag aatcaaaaaa agaggccggg
    42541 aatatgaaca gctagaacaa gatcctgaac tctattccta ctataaagaa ctcaatcgac
    42601 gatacgaaga gtggtatgaa cagtttgata tatgtcccaa aatagtgatc gatggggaca
    42661 aatatgattt cgtagccgat ccggcatgtg gagaacaaat cgtacaagaa atcaagatgc
    42721 gggcaaagaa aatgacagaa gaggcagatg cccagtcgat ccgctacact acaaataacc
    42781 cgaaagcaaa gggaatcttt acaactaaat aatataaaaa ctaagcaaat tgctcactcg
    42841 cagatatttc tctgtgagca gcatcttgct tagttttttt gattcattga gatcaaatag
    42901 tgaaccgatt cgtatgataa aattgccata aatgaaatgt ctgagtgagg gccatgatca
    42961 aaaataaaat ggaaacgatc aaaatcgaat gggcgccaaa cagatgatga cagaaaacaa
    43021 ggacaatagc tcctgcgata aaggcaagaa cgacacttag atagtggaaa aatcgatcaa
    43081 aatctgcttt ttctttagaa atacaataat catataagtt cgttgctacc ccttttaaat
    43141 ttccagttgt aaataaatta gtgtaggcac gcccgttgat tttatcaaaa gctacccact
    43201 ggatcgatgc agtaaaagca atcaaaatcg ttactaatgc gggatgctca tggacgggtg
    43261 tcaaactaac aaagaaaaaa atcactgctt cataatatag aacaaacaag cgccagaaat
    43321 gtttttcttt ttttctaaaa taatcaatta gatactttgt tagaagaatc cctaagaaaa
    43381 agaaaagggt agacaatact tttttaccaa cacttagcca ttctccgtca aatgcttgga
    43441 tgatcgatag aatgatattg ccagtttgag cagaggcaaa agcaccatat tggatataag
    43501 tataactgtc catggctcct ccgataaatg tcagcaaaag tcctattgta cgcccctcgt
    43561 gaaaggaagt ctgcattttt ttcatcacct tttatgaata tttttgtctt ccttatctta
    43621 gcataaacga ttgaaaaaaa ggaaaaattt tcagaaaact aaaatcatat cgcttcccat
    43681 tttcattttt agaaaggtct gttagtataa agaaaaaata gacaacgaaa ctttaaataa
    43741 ccaacttgaa ataaacaaat tcaaagaaga aagatgggaa ggaaatcaat cagttgaatg
    43801 aaaaagaaat tctccgatta caaaaagaat tgaaacgctg ggagaacaag tataaaaagc
    43861 ttcaacaagt ctttcggcta caagaagaaa tcattgctac ctatgaagcg gcttatggcg
    43921 aagcacccgg acttattgag gaagaagtac aagtaaaaga aagtgaagga aacagctctt
    43981 tctttaaaat gatggagaaa cgttcctata atcgcttcaa tcaagaacaa aaagcagcta
    44041 tcacatatga tatgaagaaa catcttcgaa tcatagcagg agctgggagc ggcaaaacgc
    44101 aaacaatctg tgcaaaagct gtttatctga tgacgcaaaa acaagtagac gaagaacgca
    44161 tactaatgat cacttttaca aggaacgctg caaatgaact aaaaaaacga gtagataatt
    44221 tcagccagag aaaaaccgct gtccatatcg gaacattcca tagtatcttt tttaggatat
    44281 acaatgaaat ttgtcgaaaa tttcctgaag tagcaatgca aggaatacaa ggagactttt
    44341 caaaggatac tgcacaaaaa gtgaatgctg ttttgcaaca gctgatacgt aaatataatc
    44401 tttatatatt tgatcaatat ggagaaaaaa cgattgcttc tcggttagac tattggcaga
    44461 atatgaacct ttcagtagaa gaaatggttc agttagttaa agaaaaatat gattccattg
    44521 ataagaatgc acgacagccg atatcagaac gactgtatgg attgctcacg gaactccaag
    44581 aacaaaaaag aagccagcaa ttgctagagt tcaatgatgt actccaaaat ctgaaaagcg
    44641 ctttggagaa tgaggagatc cagcattata tcggccaaaa atatgattat ctattcattg
    44701 atgaatttca agatactaat ccacttcaat gggaaatcgt gaaacggatg acaaaggaaa
    44761 acaggatcaa gttgattgtt gttggagatg atgatcagag tatctatgga ttcagaggat
    44821 cagagccagc atacatcaag aactttgaaa aagaatatcc taccaaaact cttttcttat
    44881 taactaatta tcgttctcgg gcagctattg tccaaggagc aaatagattg atcagctata
    44941 ataaaaatga tcggatacca aaggcgatga ttccagcaca aaaagaagca ggtgttatgg
    45001 aagcctattt gttttctgat actaaagcag aaagtcagtg gctaatcaaa caaattcaga
    45061 catttattca aaaaaatggg aaatataagg aatcaatcat attataccga tcagcttcac
    45121 aaacgcagca gcttgtccaa tctctgctga aaacgaatat tccttttgta ctggaagcag
    45181 atagtccata tgaaggtatc tttggtatca agtctttcca acatttctat caaaaaattg
    45241 ttcattggca aacagcactg aatctgcaaa agaaaaatca ggcctaccag caattgctcc
    45301 ggcagctgat ggcagactgc tacctgaaaa aaagtgaatg cgatcaatac ttttctccta
    45361 aatatcaaaa tatagccgtt gcagattata ttttgtcgat aagccccaat ttaaaatcga
    45421 aatctgcaga aatccgagag tttgaggaag cgttgcgtaa aacagaccag catcaagaaa
    45481 tctattcgct tgttcaaagc tatcttcatc tgccacgtat agctaaggaa atcgaccaga
    45541 gtgatcaaga atggatacga gaagaagtaa agcagcatgc tactttcaga agactccaag
    45601 ccttatcgca agaaacccaa gcgacagcta aagaattaaa aacccgcttg attgcttaca
    45661 gacaaggaaa acttgatgca ctatgtatcc agagcattca caaaagtaaa ggtcttagtt
    45721 accgaaatgt ctttttgatt ggttgtaacg aaggaagttt gccttataac ggagcgacag
    45781 aaaagaaagt aatcgacttt aatgaaataa aagcagaacc agtaacgacg atagaagaag
    45841 aacgaagact attttatgtt gcgatgacta gagcaagaaa tcgtctgtat ctatgtgtac
    45901 ctcaaatgaa aaattcaaga aaattgaaag tatcgagatt tatcaaagaa accgggagtc
    45961 gtgtgaaaaa aggaaagtga tagaagagag ggtgtgacag aagtggtcag tttcaagaaa
    46021 gaagaaggaa tgtacgaaaa ttgcttctca aatttttgtg aaatttcagc ttattttcga
    46081 agaagctatt tctggaacac agtttattcg gaaaagggag gtgtgacaag aattttgtca
    46141 cacctccctt atttattttg aaaatgagaa tactattatc aaaatagaga atatatagaa
    46201 aaattctcat tattcaaacg taaataatca
//
LOCUS       DAZUKC010000014        45995 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000014 DAZUKC010000000
VERSION     DAZUKC010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.014"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..327)
                     /locus_tag="ACG80T_000717"
                     /pseudo
     CDS             complement(13..327)
                     /locus_tag="ACG80T_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(581..2216)
                     /locus_tag="ACG80T_000718"
                     /pseudo
     CDS             complement(581..2216)
                     /locus_tag="ACG80T_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2539..3018)
                     /gene="rlmH"
                     /locus_tag="ACG80T_000719"
     CDS             complement(2539..3018)
                     /gene="rlmH"
                     /locus_tag="ACG80T_000719"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFS4840393.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            3446..4735
                     /locus_tag="ACG80T_000720"
     CDS             3446..4735
                     /locus_tag="ACG80T_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFS4840394.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(4774..5379)
                     /gene="ytpR"
                     /locus_tag="ACG80T_000721"
     CDS             complement(4774..5379)
                     /gene="ytpR"
                     /locus_tag="ACG80T_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFS4840395.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(5584..6048)
                     /locus_tag="ACG80T_000722"
     CDS             complement(5584..6048)
                     /locus_tag="ACG80T_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS4840396.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(6070..6390)
                     /locus_tag="ACG80T_000723"
     CDS             complement(6070..6390)
                     /locus_tag="ACG80T_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFS4840397.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(6534..7613)
                     /gene="pepA"
                     /locus_tag="ACG80T_000724"
     CDS             complement(6534..7613)
                     /gene="pepA"
                     /locus_tag="ACG80T_000724"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFS4840398.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            7813..8163
                     /locus_tag="ACG80T_000725"
     CDS             7813..8163
                     /locus_tag="ACG80T_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFS4840399.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            8386..9333
                     /locus_tag="ACG80T_000726"
     CDS             8386..9333
                     /locus_tag="ACG80T_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4840400.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9390..10808)
                     /gene="pepV"
                     /locus_tag="ACG80T_000727"
     CDS             complement(9390..10808)
                     /gene="pepV"
                     /locus_tag="ACG80T_000727"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFS4840401.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            10957..11802
                     /locus_tag="ACG80T_000728"
     CDS             10957..11802
                     /locus_tag="ACG80T_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFS4840402.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            11806..12246
                     /locus_tag="ACG80T_000729"
     CDS             11806..12246
                     /locus_tag="ACG80T_000729"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFS4840403.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            12296..12490
                     /locus_tag="ACG80T_000730"
     CDS             12296..12490
                     /locus_tag="ACG80T_000730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840404.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            12626..13311
                     /locus_tag="ACG80T_000731"
                     /pseudo
     CDS             12626..13311
                     /locus_tag="ACG80T_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(13719..14408)
                     /locus_tag="ACG80T_000732"
     CDS             complement(13719..14408)
                     /locus_tag="ACG80T_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840405.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(14383..16519)
                     /locus_tag="ACG80T_000733"
                     /pseudo
     CDS             complement(14383..16519)
                     /locus_tag="ACG80T_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
     gene            complement(16567..17904)
                     /locus_tag="ACG80T_000734"
     CDS             complement(16567..17904)
                     /locus_tag="ACG80T_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFS4840406.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(18156..18515)
                     /gene="rplT"
                     /locus_tag="ACG80T_000735"
     CDS             complement(18156..18515)
                     /gene="rplT"
                     /locus_tag="ACG80T_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFS4840407.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(18568..18768)
                     /gene="rpmI"
                     /locus_tag="ACG80T_000736"
     CDS             complement(18568..18768)
                     /gene="rpmI"
                     /locus_tag="ACG80T_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFS4840408.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(18840..19364)
                     /gene="infC"
                     /locus_tag="ACG80T_000737"
     CDS             complement(18840..19364)
                     /gene="infC"
                     /locus_tag="ACG80T_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFS4840409.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(19408..19533)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            19686..20318
                     /locus_tag="ACG80T_000738"
     CDS             19686..20318
                     /locus_tag="ACG80T_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFS4840410.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(20320..21225)
                     /locus_tag="ACG80T_000739"
     CDS             complement(20320..21225)
                     /locus_tag="ACG80T_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS4840411.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(21310..21969)
                     /locus_tag="ACG80T_000740"
     CDS             complement(21310..21969)
                     /locus_tag="ACG80T_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFS4840412.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            22285..23229
                     /gene="mvk"
                     /locus_tag="ACG80T_000741"
     CDS             22285..23229
                     /gene="mvk"
                     /locus_tag="ACG80T_000741"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFS4840413.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            23222..24199
                     /gene="mvaD"
                     /locus_tag="ACG80T_000742"
     CDS             23222..24199
                     /gene="mvaD"
                     /locus_tag="ACG80T_000742"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFS4840414.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            24212..25297
                     /locus_tag="ACG80T_000743"
     CDS             24212..25297
                     /locus_tag="ACG80T_000743"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFS4840415.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            25294..26358
                     /gene="fni"
                     /locus_tag="ACG80T_000744"
     CDS             25294..26358
                     /gene="fni"
                     /locus_tag="ACG80T_000744"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFS4840416.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(26607..27410)
                     /locus_tag="ACG80T_000745"
     CDS             complement(26607..27410)
                     /locus_tag="ACG80T_000745"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFS4840417.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(27430..28272)
                     /gene="kduI"
                     /locus_tag="ACG80T_000746"
     CDS             complement(27430..28272)
                     /gene="kduI"
                     /locus_tag="ACG80T_000746"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFS4840418.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(28288..29295)
                     /locus_tag="ACG80T_000747"
     CDS             complement(28288..29295)
                     /locus_tag="ACG80T_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFS4840419.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(29302..29946)
                     /locus_tag="ACG80T_000748"
     CDS             complement(29302..29946)
                     /locus_tag="ACG80T_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFS4840420.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(30046..30804)
                     /locus_tag="ACG80T_000749"
     CDS             complement(30046..30804)
                     /locus_tag="ACG80T_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFS4840421.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(30931..32082)
                     /gene="fucO"
                     /locus_tag="ACG80T_000750"
     CDS             complement(30931..32082)
                     /gene="fucO"
                     /locus_tag="ACG80T_000750"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFS4840422.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(32164..32475)
                     /gene="rhaM"
                     /locus_tag="ACG80T_000751"
     CDS             complement(32164..32475)
                     /gene="rhaM"
                     /locus_tag="ACG80T_000751"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFS4840423.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(32477..33322)
                     /gene="rhaD"
                     /locus_tag="ACG80T_000752"
     CDS             complement(32477..33322)
                     /gene="rhaD"
                     /locus_tag="ACG80T_000752"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFS4840424.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(33336..34592)
                     /gene="rhaA"
                     /locus_tag="ACG80T_000753"
     CDS             complement(33336..34592)
                     /gene="rhaA"
                     /locus_tag="ACG80T_000753"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFS4840425.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(34589..36049)
                     /gene="rhaB"
                     /locus_tag="ACG80T_000754"
     CDS             complement(34589..36049)
                     /gene="rhaB"
                     /locus_tag="ACG80T_000754"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFS4840426.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            36202..37071
                     /locus_tag="ACG80T_000755"
     CDS             36202..37071
                     /locus_tag="ACG80T_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS4840427.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(37197..38096)
                     /locus_tag="ACG80T_000756"
     CDS             complement(37197..38096)
                     /locus_tag="ACG80T_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4840428.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(38261..39085)
                     /locus_tag="ACG80T_000757"
     CDS             complement(38261..39085)
                     /locus_tag="ACG80T_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS4840429.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(39096..40391)
                     /locus_tag="ACG80T_000758"
     CDS             complement(39096..40391)
                     /locus_tag="ACG80T_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFS4840430.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(40407..40886)
                     /locus_tag="ACG80T_000759"
     CDS             complement(40407..40886)
                     /locus_tag="ACG80T_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFS4840431.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(40892..41920)
                     /locus_tag="ACG80T_000760"
     CDS             complement(40892..41920)
                     /locus_tag="ACG80T_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFS4840432.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(41937..43043)
                     /locus_tag="ACG80T_000761"
     CDS             complement(41937..43043)
                     /locus_tag="ACG80T_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS4840433.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(43272..45869)
                     /gene="adhE"
                     /locus_tag="ACG80T_000762"
                     /gene_synonym="adhC"
     CDS             complement(43272..45869)
                     /gene="adhE"
                     /locus_tag="ACG80T_000762"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFS4840434.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT        13307 a         9770 c         8326 g        14592 t
ORIGIN      
        1 aattcttctc gtttacctca attcattttc aaggggaatc agctcatcat ctaacatttt
       61 acgccatgct cgcttcattt cttgtgggta tttaaatggt ttcgcttctg gatgtttggc
      121 tttatatcct tcgtaatcct tactgttgat aattgctttc atattaggaa ttggatcttc
      181 acctggcata tattgcagat tagaagcatg taattttttc tcagaaacaa accactcatt
      241 ggaaaagaca tgaatcaaat catcacttac ttgtgtaaaa tagcgacgtt taatgacaaa
      301 gatatcttca ttgctatcta gtgtaccttc atttctttgt gcttaaattc aaagataatt
      361 ttctcaataa agatttttga ttccagtaag attcctttac tccttttaca acatagcgtt
      421 gtcctttgtt ttgaaacatc ttcttcccct ctttgccatt aaaaatagcc caattctcta
      481 taaagaagat taagcttcat tattataata taaagtttaa atttggttta ttacttactt
      541 tttcttattc tctagtaagc taatattgaa aaaaatgtaa tcagatcatt atttttatat
      601 gttgtaactt cctgataaga aaactaatta aaagaaagag gtaactacat aaactaatta
      661 aaaaagctaa cttcatagtt ggaggataaa tggaagaaac ttcaatgatt gtttctcctt
      721 gtgttccttc aatcgacact agtcctatta tcaatctctc ttttttgtcc atttactttt
      781 gcgactgttc cccaataatc aaatacagga agcgttattt tttgtccttt agattcatgt
      841 actgtataga ctattttcga atccatcact tgataaggca tagtatgcca ttccttgcca
      901 attcttactt catgattctg caaatgttct ttgtattgta atgcttccgt gggtgcatag
      961 tcataaagtc ctccgggaag gagtgtcgtg taacgaataa gattttcttg attcattacc
     1021 aagtgatctc tctgagtgac aactttatgt tcatagtttg cttttgttgc agcagataca
     1081 gccaacagta ataaaacaat tgccgttata ttaactactt ttctttgctt agctgatgtg
     1141 ctcttcatga aaacgaacga taaaatcaac gagccaaaca gtacttgaaa gcctaagatt
     1201 cgccaaggaa attgaatcat ttgaatgggt gtattttgta ataaagacca tggaaaaaat
     1261 ttggtggata agagccaaga gactaccgca ctaaggtaaa tcactttttc tttgcgatta
     1321 aatcctttaa aaaagaagag tggacagatc aaagaaataa taccaatgat tcctaccgta
     1381 aaggttgtca ggtcattatt catcgcacca atcaaactat ccgagacatt taatgcttgg
     1441 tgttgtaagg tcgttttatc tggctcatta atcgattgaa ccatgatttg tcggaacatc
     1501 ggataccaaa agtatgctgt taaaagaatc gtcattccta ctgcctttga caactccaca
     1561 atcaaccgga tgctttcttt ttttcttgtt tcacttacta caaagccgcc taagactagt
     1621 aagccaacgg ctattacact cataaaagtg gaaagaacat gtgtataaat gagtaagctc
     1681 atactgactg ctaatggaat tgcacatggc tttttctcat aaatcagtgc gtaagcataa
     1741 tacataacta tcggtataac cgtaagtgca atcgcttcag caatagcaga acgatgataa
     1801 atatcaatcg tacggtaccc ataaaatgta tataaacagc taaatataaa agccgcatcg
     1861 attcttttta aaaattttag tccataatga aacatcagca tgatagtaca aacattcaat
     1921 agccaaacat ataagatata actaacgatt aaattattac ttatccaata aaaaatgact
     1981 gccggaaaaa aggtcaagta gggataaaaa taattcactc cgctcccata atgattaaac
     2041 gtcgtaaaat tgattggacc agaaaaaatt gaagataacc ctttgactct agcaaaatgg
     2101 aaggcaatat cccccgatgt agaaatagta tgaacatttc taaaataatt gattgaataa
     2161 agtccagcaa taattgctag gagaatggac aatattattt ttttctttac ttgcattact
     2221 taattatata ctcctttcat ggattgcttt atctagtata acttaaattt taaataaatc
     2281 gttatttatt tttactctca cttaaaaaag cactaaaatt ataagagagg gaggtaatgg
     2341 tctgaacacc aagaattgtt ccaacatcga attgcttaac taatgagttc aaagcttaat
     2401 tatgtggctc ttatatttac cgagcgtgga ttgcgatctc tttagcgaaa caaacatctt
     2461 tcataaaaaa aacagccata tccgctttct tggatatgac tatttactac tactttacta
     2521 tctttcattc ttcttgtctc acttatgata aggcgccccc gcattgatcc ggaaggcacg
     2581 gtagatttgc tcgacaagga tcagacgcat caattgatgt ggatacgtca tttttccaaa
     2641 agagatctga gcattgcttc ttttcatgac ggcttcgctg agtcctaaag agccgccgat
     2701 aacaaaaaca aggtggcttt ttccttggat acctaattgg tcgatttgtt tagcaaaaga
     2761 ttcacttgta ggatttttcc cttcaattgc taaggcatag acgtattctt gttctttgat
     2821 tttcgctaag atccgttccc cttctttttc ttttacttgt ctcatttgcg cttcgcttaa
     2881 attctccggg gctttttcgt caggaacttc gatcaattcg atttttgcat aggcattcag
     2941 ccgtttgaca tactcattga taccttggat caggtatttt tcttttaatt ttcctactga
     3001 gataattttg atattcatgt tttctctcct ttggtcttca tgctcacttt gtttattata
     3061 atcaattttt tcgtctatat gtttattttg gctatttttg ttaaaaagat ttccccaaca
     3121 gttatgcaca acatccacag gtttatccac agaaaacagt gttattttac cttatttcat
     3181 cacattcatt aggcaaacat acgttttata agaatttatc cactttatac acaggctgtg
     3241 gatatgtttt atcccttatt tttccaatcc tatccgcgta gttttacaca gtatctgtgg
     3301 aaaaagaaaa tcaaatattt tcatctgaaa acaatgattt ttcctaaagt ttttcataaa
     3361 aagttcacag tctcaaagta gactaaagtc aacaaataag actatactaa cagtagataa
     3421 atgaatgtaa ttggaggagg aacacatgga tagaaaaaat gtgacaccaa agatgaaaaa
     3481 aagtaaaaat aatagcctct ggcgtaagtt aggtctgggg cttgttggag ggatcgttgg
     3541 aggactactg acagctggca ttttttatgc agcgatgggc actggcaaca atagttctac
     3601 tgctacaagc ggcaatcaga attctgccgg tgaaacagtt gtggaaaatg tcaaagtcaa
     3661 tgtagattct gatatcacca aagcggtaga taaagttcaa gacgctgtcg tttctgttat
     3721 caacttacaa agtcaaaacc aatcaagcgg ttttggcgga ctattcgggc agcaagaaga
     3781 aagtagcagc tcagatgata gtaatttgga agcttatagt gaaggaagcg gcgtgatcta
     3841 caaaaaagac gggaatactg cttatgtagt cacgaataat cacgtagtag atggccagca
     3901 aggtcttgaa gtcttgatga aagacggtac gaaggtaaaa gctgaattag ttggaacaga
     3961 tgcttattca gatttagcgg tgttgaagat caattcagac aaagtcgaaa cagttgcctc
     4021 ttttggtgac tcaagtgctc tgaaggttgg tgagcctgcg attgcgattg gttccccact
     4081 aggatctgaa tatgcaaatt ctgtcacttc aggaatcatt tcttccttga accgacaagt
     4141 gacaagtacg aatgaatcga acgaaacagt caatatcaat gcgatccaaa cagatgcagc
     4201 aatcaaccct ggaaattctg gtggtccttt agttaatatc gaaggacaag ttatcggtat
     4261 caactcaagt aagatcgcaa gtacttctga atctacttct aatgtaagcg tagaaggaat
     4321 gggatttgct attccaagta acgatgtagt gaatatcatc aatcaactgg aaaaagacgg
     4381 aaaagtgaca cgtccagctc taggtatcac aatggtcgac ttatctgccg tttctactca
     4441 gcaacaagaa caaatcttga agattccttc atctgtaact aacggtgtca tcgtgacaaa
     4501 tgtccaaaca gctacaccgg ctgaaaaagc tggattgaaa caatacgacg tcattactaa
     4561 gatcgatgac aaagaagttt cttctggtgt agaacttcaa tctgctttgt ataagaaaaa
     4621 agtgggagat tctgtcaaaa tcacttacta tgaaggctcg aaagaaaaaa caactactgt
     4681 tgaattgacg atcgatcaat cggcattaaa acaaaatcaa aatagtgaaa attaataaaa
     4741 aagaggttgt gacaaagact tgtcacaacc tctttatttt ggaaatgctt ctccgactac
     4801 ggcatcctca ggcaatacta agattccttt ttttgctgga gcatttggca aatgcaattc
     4861 acgagcggaa cagatcattc catagctatc tacccctcgc aaagttcctg gccagatcat
     4921 cattccatct ggcatcatag ctcctggttt tgcaacgaca actttttgtc cagcttcgat
     4981 attgggtgca ccgcaaacga tttgcaatac tgtaccattg tctacttctg tctcagtaat
     5041 cgataaatgg tcagaatcag gatgtgggac gcatgttttt acgtacccga caacgatctt
     5101 cggttcatga tcagcttcaa gctgttcttc gaatcctgct tctttgatca atcgattcaa
     5161 tttcgctaat tcctcatctg ttaagcttac ttggccgttc ccttcgattg ttagatgatc
     5221 ggagatatgg aaaaagttcc aagcaacaac ttgaccttgt tcatctttga tccgagcaac
     5281 attccatttg cgttcaacag tgttttctgt tcctttatcg tcggctataa taaccatcaa
     5341 ggtgtctccg acgtgtgctt tgttataagc aaaaatcatg ttctttcctc cattatcctt
     5401 tgctgagatt tatcttaaca aaaaattgca ttcactgcat gaaatttttt tcatgcttta
     5461 tttccgaaga agcctcttct ggaacaccat ttattcgtaa caagggactg tgacaagact
     5521 tttgtcacag tccctttact gttttagtta attgattaaa aaggtcgtac aagttccact
     5581 tgcttaatta cttggctgtt cttcttcgtc agaaacgatc aagacatcac aaggtgaatg
     5641 acgtatcacg tagctggcta cacttcctgt cataaagcgt tcgactgcat ttagacctga
     5701 ctgcccaacc atgattagat cgatctcata tttttttggc aatttgactg ccatggattc
     5761 acgaacagaa ccatatgcaa taattccttc tatatttgtg taatcaacag atttcccata
     5821 ttctttacat tcttcgatca tttccttggc tgtttcagtt tgctgatcga ctagattttg
     5881 atcaataggt gcatagccca taaagttata aaattgctga tcgattacgt ttgctacata
     5941 tactttccca ttattacggc gagccacttc taccgctttt ttgaaagcag caagtgcttg
     6001 ttcactaccg tcgataccta ctaaaatatt cttatattct tgattcatct tgacccctcc
     6061 taacagatgt cagccttttg ttacactttc taggaacgat tcgatttcct gttttgtttt
     6121 acgatcttta ttcactagac gacctaattc ttttccgtct tggatcacga caaaacttgg
     6181 gataccaaaa atgttccatt cagcggctac atctatgtac ttgtcacggt ctacttcaat
     6241 aaattgccaa tcagaaaagt cattttcaat ttcaggcatt tgcggtttga taaaacggca
     6301 gtctccgcac cagccggcag taaagaaaaa tacactcttt ccagtttcta cgtagcctgc
     6361 taattcttct aaactatttg ggataatcat tctttgtcct ctactttctg tttatgtatt
     6421 tttcaattaa attatacacc tttctttcga actaacaaac attgatcctc tttttataaa
     6481 aaaaatctgg caaaaagcaa tcttttgcca gattttatag tttcactatc gcattatttt
     6541 ccgtatacga tcgtatttac tgttgattta tccaaacctt tcagtgtttg aatcaacatt
     6601 tctcgtgcag cttcaaaatc gcgaatactg aacatcgttt gatgcgtatg gatataacgt
     6661 cccactacgc caatcactgt acttgggatt ccttcgtttt gtgtatgagc agcacctgca
     6721 tctgtaccgc cttttgaaac aaagtattga taagggatat tgtttgtttc agcgatatcc
     6781 agcaagtact cgcgcaaacg aggaagcatg atcaaacctg gatcttggat acgcatcaat
     6841 gtaccttctc ctaaatgtcc gaatgtgcca tttttcgtca ctgtatcatc tgctgcagag
     6901 cagtctactg caaagaacag atctggcttg aatttattga cagaagcttt agaaccacgc
     6961 aagcctactt cttcctggac attcgcgcct gcaatcaatg tatgacctaa tgtttcattt
     7021 tgcaacgctt ctaaagcttc taataccatt gtacagccat agcggttgtc ccatgattta
     7081 ctgataatat tctttccgtt ggctgttttg atcgtttcag cgtaaggaac gattgtgtct
     7141 cctggaagga caccgtaact cattgcctct tctcttgatt caaacccagc atcgaacaaa
     7201 atatcggtta cttctactga tttttgtccg cttgttcctc gcaataaatg tgggggaacg
     7261 gaagaagaaa tacatggata attcttacca gtagaggttt taagtgtaaa acgctgagca
     7321 gaaacaacgt aaggattcca gccgcctaga ggtacgactt taaaaagccc gttttcttgg
     7381 atttgtgtca gcatgaatcc gacttcatcc atatgtgctg cgaccattac acggggtgca
     7441 tccgcttctt catgatggcg cagaccaaaa atgccgccca aaccatctaa ctgtaactca
     7501 tctaccagcg gttccatatg actttccata taagcgcgaa catcttgttc aaaaccgctt
     7561 gttccttgta attctgttaa ctctttgatt cgttgaaatg ttttttcttc catatgctat
     7621 tcgtctaaat ttcagactaa actcctctca gacttatatt gatctgcgga taaaatatga
     7681 ttttttctgc agaccgatcg tatttattat agcttatttt ttctggtagc ggttatttat
     7741 ttcaaagaaa atatgagagt gtggtaaaat aagcacaagg agtaacaagt gagaagaagg
     7801 aggtatgtcc acatgtatga agaaagcgaa ctaagctatt ttaaaggcgg gttagcagtt
     7861 ggaatcggtc taggattatt gagcggggta gcttcaacga tttggtatca tcgcaaaaaa
     7921 gtattgtcag cagaccaagt acttgaaaat atcaagtcat cttttctaaa agaaggtgca
     7981 atcgaaggtt cttggatctc ttttgaaaag gaacctatta ggaaattcgc cgttcggtcg
     8041 agtgcttatc ggggaggtct ttctcgaata gaagatggcg aaactgttta ttatgaattt
     8101 ttagcagatg ctcatacagg aaccgtatta gaaatttcaa gaaaaaaagg tgcaacagat
     8161 taatacgtca aaaaagtgag agaacgaata cgcacccact attcaaaaga gaggtatcat
     8221 gtccttcttt tgtagtgggt ggttttttag atagagcttt ccaaacatat attccagtat
     8281 tttcttttta aataaatgat atttagcggg aaaaactagc atttcctttg attatgaggt
     8341 atttttaatt tagcttttgt tttctatagt taaggagagt gaaaaatgga acaacaagaa
     8401 aaaattgctg ttctgattcc ttgttacaat gaggaagcaa caatttcaac agtcattgca
     8461 gactttaaac gagaattacc agaagcagat atttatgtct atgataataa ttcgaccgat
     8521 cgaacatacg aattggccgt tcaaggtgga gcaatcgtaa aaaaagaacc tcgtcaagga
     8581 aaaggaaatg tgatccgcca aatgttcttt gatatcgatg ccgactatta tttaatggta
     8641 gatggcgatg atacttatcc tgcagaagct gtacatggtt tgctcgagaa attacgttct
     8701 ggtgaagcag atatggtcat tggtgatcgc ctatcaaatg gaacctactt tgatgagaat
     8761 aagagacctt tccacgattt tggaaataac cttgttagaa atacaattac tagaatgtat
     8821 aaaacaaaaa ttcttgatgt catgaccggc tatcgaggat tcaatcgtat ctttgtcaaa
     8881 agttttccta ttatgagttc tggttttcaa atcgaaacgg aattgacgat ccatgcttta
     8941 gataaaaaat tcaagttcgt tgaaatccca atcgattatc gtgatcgtcc tgaaggcagc
     9001 gaatcaaaat tgaatacatt ctctgacgga ttcaaagtga tcatgatgat tattaaaatg
     9061 tttaaagatt ataagccttt gcttttcttt gggatttgga ccattttctt ctttttattt
     9121 ggattggtca cggggattcc tgtgattcgc gaatatatgc tgacaagctt tattacacgc
     9181 atcccttctg cgattttaag tactggattg atgactttag cacttctttc attagtaaca
     9241 gggttgattt tggatacagt cgtcacgaac gctaagaaag aatatgaatt gaatctatat
     9301 catgtttatc atgaatacca cagtagaaag taaaaaaagg atcaagactg caaaaataat
     9361 ttgcaattct tgatcctctt tttaattaat tatttcgcca aacgatagat ggcatctgca
     9421 tagattgctg ttgctctgaa caagtcatca agactcataa attcgtttgc ttgatgcatt
     9481 gtatcagtat atcctgggaa catcgctcca taagcaacgc cacgtttcaa caaacgtccg
     9541 taagttccgc caccgatgat ttgttcgtag cctttttctc cagtgtggtc ttcatagact
     9601 tgcaataatg tagccaccaa tggatcatcc attggtacgt aatgtggttc cattacgcga
     9661 gcacctcgag taactgttgc gccttcttgc cccactgttt gttcgatgtc actttgcaat
     9721 ccgtctactg ttacaccttt tggaaaacgg aaattcatat tgatgaagtt cgcttcctct
     9781 gtaccatttt cttcaaaagc aaatagtcca gcattcattg tcaatttgcc cattttttca
     9841 tcttcataag cgactcctag tttttctcca taaaaatctt catggacata agcagctgct
     9901 acatggataa attgtttagc agatcctagg aattcatagt tatctaagaa caatgccaag
     9961 aaagaacctg cattgatacc agattgtggg cttgcaccat gtgcgccttt accaactaat
    10021 tcgattttta catacgtatt gtccgcttca atcgttccgc ttactggatt tgcttcgatg
    10081 aattggtaaa atgcttcttc catcgcgata gctgcatctg cacttggtac ttgaagtgct
    10141 gcagttgctg ttcctggcac catattttcg cgaagtccgg aaacaaaaga tttcaagaca
    10201 taatctcctg cattgtcgcc tttgaagcgt aaagctaaag aaacatttcc tttttcacca
    10261 ttgatgattg ggaattcagc atctggtgag aaaccaaaat ctggttcttc ttcatgttcg
    10321 aaataataag ccatatcgcc ccagccgctt tcttcatcgc tgccgactac gaagcgaact
    10381 ttttttgata gtttcaagtc aagttcttta atgatcttca tcgcatagta agctgccata
    10441 cttggacctt tgtcatcgct tgatccgcgt gcaaagatct ttccatcctt gattactggt
    10501 tcataaggat ctgtgtccca gccatctcct gcaggaacga catccatgtg tccaaatatc
    10561 cctagcgttt cgtctccttc gccaagatca atatgccctg catagttatc gacatttttt
    10621 actttaaatc catcacgttc gccataagct aacatatgtt tcaatgcgtc gcgaggtcca
    10681 ggtccaaatg gtgcatctgg tgttactttt gagtcatcgc gctcgctgtt gacacgtaaa
    10741 agatttttca agtcttcaag caagtcttct ttacgtgctt ctacttcttt ttgccaatca
    10801 attgtcataa agttaatcct ccacttattt aagtcttcgt gttcatgata ccacaaatta
    10861 cagaaaaaaa tagttcaaaa aatctgacaa ttccttttct tatctgtttt tctctaaaaa
    10921 aaatatacgc tataatgagt aaaaggaggt ttttagatgc tgataaacga acaactagtg
    10981 aaaagagtca tcacacctcg tccaaaaatt tcacataaag gaacatttgg acgtatcgtt
    11041 ttgattggcg gcaatgaaca atatggcgga gcaatcatta tgagtgcttt agctgccgta
    11101 cacagcggag cgggtctcac gactgtagta actgatgaaa aaaatcggag tgctcttcac
    11161 agtcatctgc cagaagcaat gattgtagtt tggacggata ctgaccagtt tctttccgta
    11221 ataaagcaag cagatgtact cttgattggt cccggattgg gtacttcttc taagagtttg
    11281 gaacgtttca aacaagtact agaaaaccaa tcaaaagaac aatggctggt gattgatggc
    11341 tctgctttaa gtttacttgc tgctaatcat cttttgctac cctatcccga acaaactgta
    11401 cttacacctc atcaaatgga gtggcaacgg ttgagttctc taccgataca agaacaacgg
    11461 gataaagaaa acaaaaagaa gcaacaggaa atcggtgctg tgctcgtttt gaaaagtcat
    11521 gagacaaaaa tttatggtca ccagcacttc tacaccaatc ctttaggttc tgctgctatg
    11581 gcaacgggcg gcatgggtga cactttagct gggatgatca caggattttt agcccagttt
    11641 cctcaaaatg accaaacgat tgcagcagct atttatcttc atagtttcat tggagaccag
    11701 ctggcaaaag aacaatatat tgttttgccg acacaaatca gcgaagcact tccgaaatgg
    11761 atgaattatt tttgtaaaaa atcaatggaa aacgaggcat gagtcatgga gaaatttaca
    11821 ggttatgtgc gagaaccttt ttattatgaa acagatcaaa tggggattat tcatcacagc
    11881 aattatatcc gctggtttga agaggctcgg gtagccttat tggtacatct agggtttccc
    11941 tataaaaaaa tcgaagaatc tgggatcatc atccccgttc tggaagtaaa ttgcagctat
    12001 aaagaaatga tccattacgg agaaaaagtg agcatccatc caactatcca aaagtatacg
    12061 gggacacggt tggattttag ctacgaaatt gtgggaactg aagacaaaaa attaaaaact
    12121 actggcagta gtaaacactg ttttttactt tctgattcgc aacgattagt gaagctgagc
    12181 aaagtcaatc cggagttaga tcagttattc caagcgtacg cttctttgga tgatgtaaat
    12241 tcttaataaa gaccgtctaa gcatttttct tgtattgtgt cgagtagttc gtataatgaa
    12301 aagaaaaaag caggtgatcc ccatgtatgt cgttaaagta atgcacggtt acatcgataa
    12361 aactggctgt cgcactcggg aaaaaaactt agacaatctg ctcattttca aagacaaaaa
    12421 agaatccgaa gcttttgcta aaagaatcgg cggtcgtgtc aaaccgatcc aagaggtacg
    12481 accagattga ttcaatattt ttcttttcga ggctctaata ggcatgccta tcagggcttt
    12541 ttttgcagac tgtgttttta tgcccacacg atttgtaagc gatttatgtt ataatagatt
    12601 atatctgcat agaaaggaag gttaaatggt tatcaataat ttgcaacagc aagcttatga
    12661 atctatacgt aaacaaatca tttattccga gttagcacct ggaagtaaaa tctccgataa
    12721 agtgttagaa gaacaactag ctatcgggcg tacaccgatt cgagaagcac tgatccaact
    12781 gcgcaatcaa gagcttgtaa aaaccattcc acagtccggc acttatgttt caaaaatcga
    12841 tatccgatct gcttcattag cccgttatac gcgggaaaag ttggaaaaac cgatcctgca
    12901 agaatgcagt gcgaaaatga ccaagcaaga tcaagctaaa ctagaaaaaa tactagaaca
    12961 aaccgatcag gcagtaatcg ctcaaaataa aaaagctttt ttgatttgga tactgatttt
    13021 caccgaactt gttatgaaat tgctggaaaa cgagaaattt gggattggct agaaagttat
    13081 agtacgcact tgaaccgttt cagatggtta cgtttgacga tttcagaatt agactggggt
    13141 cgagtattag atgaacatca gacaatgctt caatcaatga ttgatcataa ctttgatgaa
    13201 gtcggatttc tttgcacgat gcatttacat atgattatcg aggagcaaga atacgtgatt
    13261 cataattacc ccgattattt tgaagatatt caggacagac ccatcaaata agaggttttg
    13321 gaatcagcgt tttgacctga gtattagaga aagaaacggc aaaatagttc ctcatatttt
    13381 gccgtttttt ttgagctaat accgagggtc agctgattgc acaccgttta ttgagaggtt
    13441 gtgaaaaagc tgccctgcat caagtaataa gaacagccaa gtaaaaatag cttcttatgt
    13501 ttttaacttg gctggactta ttaccgcaga tgcaagcttt tgaacaccgt tttccaagag
    13561 gtcgtgagcc tgccgttcat ctccgagtca caaggaacaa tttcaaaaaa cagcttctca
    13621 tgtttgttga atattttgac ttgtgaccga ggagttgaca ggagaacatc gtttattcgg
    13681 aaaaaaagga ctgtgacacg acttttgtca cagtctcttt atttgtaatc taaatcatac
    13741 tccgtacatc ttttttctat tttttttatt tcttctaaga tcccttggct acgaatttca
    13801 ttatatggcc aaagtaagac tgcctctaat acataaggcg aaaaatcgct ttgattcatc
    13861 tcttttgaaa aactcatcgc tgctatctcg ctcaatgcac gaatgtttga ttgatgtgta
    13921 taaaaaggca accgccacag cacaattttt tcgctttgcg tataaattcc cttaacagaa
    13981 gcttcgatgt ggtgaaatag ttcatgtcca ataatcaaag cttgcgctgt ttctttagag
    14041 atcgaagcta gtcctgaaca ttttaatttt tctaacggct ccgtcatcaa tcgaatccct
    14101 tcatcttctg cataagttgc aaaagtgatt cgttcgttta tttgctctga ttcacaggaa
    14161 acgattggaa cagccatttg ttcacaaatt tctcctacat cggcaaaagg gtactttttt
    14221 ttgatgctac atgccgtttc gataccacaa tcgattgatt gttgtataag aattttctgc
    14281 ttggacaaag gaatcttttt ccaaaataaa tctcgccgaa actggtagaa tccccaaatt
    14341 tcttcgggga tctgttgtaa ttcgtttagt aactcttcta acttatgttt gtttgatatt
    14401 tccggcaacg acgatcactt cttcctccgg ttccatagta gatacttcca gatccatcaa
    14461 aagctgtagt tgttccagat ccgtcgctgt aaaatcagag actcttgctc ctaagcagag
    14521 ataaaattct ggtcgggcaa tcaattcagt ttgataaact gccatatctt cccaaagttg
    14581 ttcgacagct gtcatataat tggctgcagt tgttttcata catgaagcat gtccacgttg
    14641 gaccttgata aatccttttt gatcgatgat ccgaagactt ccagcatttt ttgaacgatt
    14701 ctgttcgccg catacataaa agaaaggtgt attttccaat aagcaaactt catttttatt
    14761 taatcgcata tcctctgcag caagctctag tgcttcttct gttgtgatct ccttggttag
    14821 atcggttgct ttcacttccg tagaaccagt agcgatagcc gttacttttg aagtctgtgg
    14881 atcgatttct acgtggactt cgatcgattc tggtgtagcg cctgattcaa tcgctttatt
    14941 catcgcttca tttttcaacg atcgtatatc ttctttactt ggcgaaggga tgatacgttc
    15001 tacaacatct cggaccattg caagtgctac accaatagaa gaaatcactt cggcgttttc
    15061 aggaatcgaa tatttcacac ccattttatt actaaagtaa gtaatcaatg aggcagcgcc
    15121 gccccctaca cctacaagag aaatctgatc tttctccaag tgatacttgt ctgccaattc
    15181 taaaatcacc ggttcaatct tagcatagga tttttccatg atctgttctg cgatgtcttc
    15241 aaccgtcgta tgacaataat cagctaatgc ttggatggct tttcgtgcag atgggacatt
    15301 tccataagaa aaatgttctt cttgcactaa tccaaggaca ttggctgcac atgtatttgt
    15361 aatagtcact tcttctccgt cttccatcac taccttgacg taatcagcag ggtctccttc
    15421 tttaggagag aaaaattcaa ccttaggacc tttgattttt tctgtctccg taaatactgc
    15481 ataatctaga ccggcaatat gtgcagaacg tggaccgaca tcgatgattc ctgactgatt
    15541 agcccgtacc atcgaaccgc ctgccacacc tagaacccga acatctaatg aagagatata
    15601 tgttgggtgg ccggtggcca cctacaattg aataatcgat tgctggacgt ccatttttaa
    15661 tgaccccgat attggttgtt gttccgccta cttcaaaata aacaccatta gacgctctca
    15721 agtacatcaa agagcccatg actgatgctg ctggaccgga aagcatggtt aaaacaggtc
    15781 gttttttcat ttcattgatt tccattactc cgccgtctcc gcgcataatc atcaaagaaa
    15841 cattgacccc tgcttctcgt acggaatctt ctgtagaggt tgccgtatct agcatttttg
    15901 gtaaaataga tgcattgatt gctgctgtcc gagttcttct tgtcaatcca tataattttg
    15961 tgatgtcaga agccatcgtt gtagggatcg attttaccga tgctgcttca taaacaactc
    16021 gttctggttc cccattatct actccaaatg ccatagaaga aaccaagacc tccgctcttt
    16081 ctctttctaa agaagaaatc gtttctgaaa cacggtctac atttaagtgt ttgacaggta
    16141 agaatgcatt gacaatctct atttttttct tattccctaa atcgatatcg ttcaatctag
    16201 tttgtcgttt tgctaaaaaa ccttctaatc caccttttgc catcccgatt acgccgactt
    16261 ttgctacgtc tccttcaatc agtgcattag tcgcttgtgt ggtagaatgt gcaacaaaca
    16321 caacatcttc tggactgata ttattttcca gcaaacaatt ttgaaaagac tgaacgacac
    16381 ctgcggctac tccacggaca tcatcgtggg ttgttttcac tgaagatttt ccgatgattt
    16441 cgtgcgtcgc attatcgatt gcgactgctt tcgtatgtgt accgccaaca tcaattccca
    16501 ttcgcacaaa acgtttcatt ttcttttcct ccattcatct aatcgttttt atcgacgttt
    16561 tgtttactag gcgttcccgt acaataagaa cgtgatcagg cataagatcg ctccgatgat
    16621 ccaaccagtt gggatggatt tcttcatata ttctcgagaa gttacttttg tataacctaa
    16681 tccccaagca acccaagatt gtgtaatatc taaatgctga ggagcaatcg ttgtgattgc
    16741 aaataacggg aacaaaaagg ctggcgacat cgttggatta acagctaata cgacagctaa
    16801 aatagctgag cctgaaccga ctagattcat tggaccgcgg aaaaatccta gtggtgtcaa
    16861 tatagcaaat acgattgcca agactaatgg tgattgcggt atccaatctc caaaaatagc
    16921 tgaaaaataa ggagaagcat acgcagctga attattgaac atcgctaagg tcatcaagaa
    16981 accaaccatt ggcgcaacat ctactgcgcc atctgtaaat aatttcgcaa actgacgaca
    17041 gatttcttta taacctccgc gcaattttcc agtaaacagc aatgcccaga tacctgctag
    17101 aataaaacct aagatgattg gaagatctaa aagaacgacc cctaaaactg gtaataatac
    17161 ggctaaccat gagatcattg gtgcgtcgcc accttcagct ggtacgtttg ctgccattgc
    17221 atagcgtttc tttttgttca ttgatatatt agcgaccgtt agtaccacaa ctaagcttac
    17281 aatcatccca atcataccaa tttggaaata gtcattataa gtatagcttt cagccgctgg
    17341 attgaaacca gcataaatcg tttgatattg tttgaaattg acgatattag caaagattcc
    17401 agccataatc gagcccataa atgagaacag tgcaaccggt actgaaatac ctaatgacat
    17461 caagattggc aacacaataa ctgcaataga aatcactggt ccgattcctg tcatcgacat
    17521 gaatataaca gcggttacaa tacatagtaa gctcatcgtg attcttggct tgtcccctcc
    17581 aagttcaact acctttcgaa tcagggtagc tgcgattccg ctatctacta gaacacgccc
    17641 gaaaaaggca ccaaagaaga catttaccaa gatcgatttg gcgtactcag ctggccccgt
    17701 cgtatagata tgcgtcaaca catcaattac tccttgccct tccatcgccg ggtttggtga
    17761 aaacgtgttt ccgactagcg ctaatcccat ccatacagta gccatgatgg caaagccgac
    17821 catcaagttg tagcctttta cacagtacca gaccatgaaa aagaatgaga taactaacaa
    17881 aataccaata ataatatcac tcatgttatt cctccgtcca ttttgttttt ctgataagca
    17941 aactaatata tcagtcaaac tcattctaac ttatgtagtt agcgctttca ttaaagggtg
    18001 accataaatg ttcagttttc aattaaaaaa ccttaattat gctattttag acttatacgt
    18061 tattgtcctg tttcatttga tggaggattg taaataaaaa aagaggaaag aacataactt
    18121 ttcgttatct tctttcctct ttataattgt ttagcttatt tagccaaagc ttcttttgct
    18181 tggtcagcca atgcagtaaa tgctgctgca tcgttaacag ccaagtcagc taacatttta
    18241 cggttgatgt cgatttcagc aactttcaaa ccgtgcatta attttgagta gctcaagcca
    18301 ttcatacgag ctgctgcgtt gatacgagca atccacaatt tacggaagtc gcgtttcttt
    18361 tgacgacgat cgcgatatgc gtagttgtat gaattcatta cttgttcttt tgctgtttta
    18421 aataaagtgt gttttgaacc gtagtaacct tttgctaatt ttagcactct tttacgacgt
    18481 ttacgagtta ctgttccacc tttaacacgt gccatgtata attcctccta ataaaaataa
    18541 atcttcagat tctgttattt gtacttctta tttcattttt gctagttgtt ggcgaatacg
    18601 tttgaagtcg ccactagaaa ccatgcttgc tttacgcaat tgacggcgtt gtttttttgt
    18661 tttgccgtgg aaacggtgac ttgtaaacgc gcggaagcgt ttcaatccgc ctttaccagt
    18721 acgtttgaaa cgttttgctg atccgcggtg tgttttttgt tttggcatga ctatatttcc
    18781 tcctcaaaat ctttcgttat ccgactatcg gctgtccaac agccagttag tcattcaaat
    18841 tacttactgt cgtttttcgg tgccagcgtc aagaacatgc tgcgtccgtc cattttcgct
    18901 ttttgttcca ctgttgcaat atccgcagtt tcttcagcta agcgattcaa gactttttga
    18961 ccaatttctt tatgggtaat ggcacggcct ttgaaacgga tagaagcttt cactttatcg
    19021 cccttttcca agaacttacg tgcattacga agttttgtat tgaagtcatt caagtcgatc
    19081 gtaggactta agcgaacttc ttttacattg atcacttttt gttttttacg cgcttcgcgg
    19141 tctttctttt gttgttcgaa acggtattta ccgtagtcca tgattcgcgc aactggtggt
    19201 ttcgcaccag gggcaactaa cacaagatcc aagtttgctt gttctgcaat attcaatgct
    19261 tctgcttttg tcttaactcc taattgctca ccatctgaac cgatcaatcg taactctcgt
    19321 gcacgaatgc cgtcgttaac catcatatcc tttgctatgg tcattcacct ccaaatatta
    19381 tgagagaaag aaaagcttac cgaataaacg gcaataaaaa aacgagttct cttacagaac
    19441 ccgcgcgtac ttcacctaat agaaccagtg aaaactatct agcccagtga cattatcaac
    19501 taaggcgaga agcgggtgct tctgctttta tttctcaacc atcatagtat agcaaaaact
    19561 aatctttttg tcaatgtttt tcatacagtt tgtcttcctt ttctgaaaat gattagatta
    19621 gcagactttc tagcagtgat tcccgcttct ttgtgctata ctaaagaaaa acgaggtgaa
    19681 cccacatgaa aaaaatcgaa cctattccac cttctcttcc tcttcttaca aagggagagt
    19741 tttatttaga agatgaggag atcttctctg gtcttctgat caacgggggt gaccaaagct
    19801 atctcgatgc caaaaatatg gtattaagag agagtcatat ccaaaaactt acgatgcaaa
    19861 aaacagtttt acgtcatttt gaatgcagta atgtcatttt tgaaaaatgt gatttttcca
    19921 atctggagtg gattggtggc agtttccacc aagtcgtgtt tcatcaatgt aaattaacag
    19981 gtacgaattt tgccgaaagt tatcttcgag actgtacttt cactgattgt attgcttcga
    20041 tggcttcctt tagtagtaca aacttgaaag ctgtctcctt cgatcattgt caattagaag
    20101 acagcgaatt ttatgaggtc acttggaaaa accttttttt gtctgacaat cagttgaccg
    20161 gttcgaactg gtttcgaact tctctgaagg gattagactt tacgactaat ttcttcacag
    20221 caatcgcttt atctcaagat tctttggcgg gactaatcgt caaccaagag caagcacttg
    20281 tgatcgccca agcccttggt ttgcagatca aagactagat caacgaatcc gccgccacag
    20341 tgcaataagc atccaaatag ataagaccaa actgatgatc aaggcgattc cccatccgaa
    20401 aacggaatgg gtaaagggta actggagatt cattccgaag aatcctgtca caatagttgg
    20461 aacggtcaag atcagcgacc agaccgtcaa aaatttcatc gtgtcattca agttgttatt
    20521 cagcaaatta ttataagtgc cagagagctg gtctaaaatc tgcgaagcta agttcgtcat
    20581 ttcaactgct tgtcgtgctt cgatcagcgc atcgtccaac tcttcttttt ccttttctga
    20641 caacttgcga taaatagcca atgccttgat ttgttctaaa agtactacat tttgttttgt
    20701 tcctgtaact aagtaaacca aaccgatctc taaatctgaa agttgcagca gattcttgtt
    20761 cgttgttttt tctcttaatt tcagattaag ccgctgacgt tcactattca cttcctcgat
    20821 taaaggaaaa aaggaatcag aaatcaaaaa caatgtacga aacaataaac tgtatacact
    20881 catgtctggc attcgttcca aaagcgatcg aattagtcga atcacgtact cggtattcgg
    20941 cgtggtaaat gtaaacaaac cgtcttcttt taaaataaaa gcgataggac tagtttcata
    21001 atgattatcg actttttgct ggtgcggaac atttgataca agcagtaacg cttcatcaaa
    21061 agaatcgtac tctacgcgcg cccgttcatt tttatctaga gaataagtca gcatctcgtc
    21121 actaatgcca tattcttctt ttaagttttt aacagctagc acatcgtttt gctgtacatt
    21181 gatccaaaaa ttacggtctt gattaaaata gtgtttttcc cgcatactct atcctcctga
    21241 catttgtttc cagtataaag aaaaaagtcg ctcaaataaa ggtttaccct ttatttgagc
    21301 aactgtaaat caaatggcta ctttggtttg gcgaatctct gctaattttt cttcgatcat
    21361 tttcaagact tcttcttttg cttgaggatc ttccacaaaa ttatagcggt ctccatcgat
    21421 ttggattttt ggactttctt tgtattcttc ataccattga tcgtaacgtc tgttcaattc
    21481 tttgtaataa tcgtatagac ttggatcaaa ggacaattgt tcatacgaac gaccacgttt
    21541 ttcgatacgc tccaacatcg tatcaaaaga tacacgaata tgaactaata aatccggatg
    21601 ttttttatgg gcagcatagg gaagttcttc catcatgttt tccagcagtt catcatacac
    21661 acgaacttct gtttctgttg ctcttcctag atctgcattt agatggaaaa gcagtgaatc
    21721 ttcataaatc gaacggtcca atacattatt gtcatctttc attgcttgtt tgatgcttgc
    21781 aaaacgttta ttcaaaaaat aaatttgcag caaaaaagca tactgttcag ggtttgcata
    21841 aaataagggc aagacttcat tatcatcaat cgattcataa aatgcctgac tatcaaagtg
    21901 ttcagccatc atttctgtta aacttgattt tccggcacca attgtacctg ctaaaacgat
    21961 cacgctcatt tacctccagc tcacttcatc actaaatatt gtgtttaact ccaacctttt
    22021 taaatatacc cccaatatct agtagtgtca attgtatttt tttctagtca aagaagaaaa
    22081 aaagtcaaaa aattcttaag aaaaaagtgt tttttcttta gaaaacgtat tcttagtttt
    22141 acttctgaga tggttttagt aaaataaagg aggaatgttt tttgagaccc ttagtggaca
    22201 aaaaaattcg tgaagcagcg caactcgttt tgggcgcttt attttttgct taaaaatcac
    22261 ttttacgtga gaaggagtta gattatggca aactatggcc aaggagaatc aagcggaaag
    22321 atcatattga tgggcgagca cgcggttgtt tatggagaac cagcgattgc ctttcctttc
    22381 tatgcaacaa aagtcaccgc attccttgaa gagctggatg caatggacga tcaactggtt
    22441 tcttcctact attcaggaaa tttagccgaa gctcctcatg cattaaaaaa tatcaaaaaa
    22501 ttattcattc acttaaaaaa acagcatgac atccaaaaaa acttgcaact gaccattgaa
    22561 agcacgattc ctgctgaacg tggaatggga tcaagcgctg cagtcgccac agcagtcact
    22621 cgtgcttttt atgattactt agcatttcct ttgtctcgtg aaatactatt agaaaatgtc
    22681 cagctttcgg aaaaaatcgc ccatggtaat cctagtggaa tcgatgcagc cgctactagc
    22741 agcttgcagc cgatttattt tacaaaaggg catcctttcg actacttttc tttgaacatc
    22801 gatgcttttt tgattgtcgc tgatacagga atcaaaggac aaacaagaga agccgtcaaa
    22861 gatgtcgctc acctctttga aacccagcct catgaaactg gacaaatgat ccaaaaatta
    22921 ggatacttga cgaagcaagc aaaacaagca atcattgaaa attcaccaga aacgttggca
    22981 cagacaatgg atgaatcaca atcacttctg gaaaagctga caatcagcaa tgattttctt
    23041 aatctattga tccaaacagc aaaagatacc ggggcattgg gtgccaaatt aactggcggt
    23101 ggacgcggtg gctgtatgat tgcattagca caaacaaaaa caaaagccca agaaatcagt
    23161 caagcacttg aagatgcggg tgctgctgaa acttggatac aaggattagg agtacatacc
    23221 tatgtttaaa ggcaaagcac gcgcatatac caacattgct ctaatcaaat attggggtaa
    23281 gaaaaatgaa gaactcatcc ttccaatgaa caacagcctt tcgttaacat tggatgcatt
    23341 ttatacagag acagaagtca tcttttctga tagctatatg gtagatgaat tttatctaga
    23401 tggcaccttg caagacgaaa aagcaacaaa aaaagtcagt cagtttcttg acctttttcg
    23461 taaagaagcc ggcctctcac taaaagcttc agtaatcagt cagaatttcg ttcctaccgc
    23521 agctggatta gcctcctctg ccagcgggct agctgcttta gcaggcgctt gcaatactgc
    23581 tcttaagctt ggattagatg atctctctct ttcaagattt gctcgacgcg ggtctggttc
    23641 agcttgccga agtattttcg gtggtttcgt cgaatgggaa aaaggccatg acgacttaag
    23701 ttcttacgct aagccagtcc cttccgattc tttcgaagac gatttagcaa tggttttcgt
    23761 tttgatcaac gaccagaaaa aagaagtgtc cagcagaaat gggatgcgtc ggacagtcga
    23821 aacatccagt ttttatcaag gctggttaga ttccgttgaa ggggatctat atcaattgaa
    23881 acaagcaatc aaaacaaaag atttccaact tctcggagaa acgatggaaa gaaacggact
    23941 aaaaatgcat ggaacgactt tagctgccca gcctccattc acttactggt ctcctgacag
    24001 cttaaaagcg atggatgcgg tgcgacaatt gagaaagcaa ggcattccat gctactttac
    24061 gatggatgct ggccctaatg tcaaagtttt agtggaaaac agccatttat ctgaggtaca
    24121 agaaacattt accaaattat ttagcaaaga acaagtaatt actgcacatg ctggtccagg
    24181 aattgcgatt attgaataaa gggagatttt tatgattgaa gtatctgcac caggcaaact
    24241 ctatattgcc ggagaatatg cagttgttga aacaggccat ccagcagtta tcgctgcagt
    24301 cgatcaattc gtgacagtca ctgtagaatc tgcacgaaaa gtcggaagta tccaatctgc
    24361 acaatatagt gggatgcctg tacgttggac aagacgcaac ggagaattag ttttagatat
    24421 tcgggaaaat ccttttcatt atatccttgc tgctattcgc ttgactgaaa agtatgcaca
    24481 agaaaaaaac attcttttat ccttttatga tctgaaagta acaagtgagt tagacagttc
    24541 aaatggccgg aaatatggat tgggttctag cggtgccgtg accgttgcaa cggtcaaagc
    24601 cttgaatgtt ttttacgcgt tgaatttatc tcagttggag attttcaaga ttgcagcact
    24661 agccaattta gcagttcaag ataatggttc ctgcggcgac atcgctgcta gctgttatgg
    24721 tggctggatc gctttctcaa ccttcgacca tccttggctc caagaacaag aaactcagca
    24781 ttctatcagt gagttacttg ccctggattg gccaggtcta tccattgagc cattgattgc
    24841 tcctgaagat ttacgtttat tgattggttg gacgggtagc cctgcctcta cttctgattt
    24901 ggtcgatcaa gttcaccgtt cgagagaaga taaaatggtg gcttatcagc ttttcttaaa
    24961 aaatagtaca gaatgtgtca acgaaatgat caaagggttt aaagaaaata atgtaacgtt
    25021 gatccaacag atgattcgaa aaaaccgaca attactgcat gatttatctg caatcactgg
    25081 ggtcgtcatc gaaacgcctg ctttgaacaa attgtgtaat ttagctgaac agtatgaagg
    25141 agccgcaaaa tcttctggtg caggtggggg cgattgcgga atcgtaattg ttgaccagaa
    25201 atctggcatt cttcctttaa tgagtgcatg ggaaaaagca gaaatcactc cactgccgtt
    25261 acatgtctat agcgatcaaa gaaaggaaaa ccgatgaatc gaaaagacga acatgtttca
    25321 ctagctaaag cctttcatga taaacaaaaa aacgaatttg acttcgtacg gatcatccat
    25381 aaccctttgc cgcaaatagc agtttctgat gtcgatctaa gtacacaggc agttgggttt
    25441 acactaagca gcccatttta tatcaatgcg atgactggcg gaagcgaaaa aacaaaaaaa
    25501 atcaaccaag atcttgcaat cgtcgcaaga gaagcagatt tgatgattgc cactggttca
    25561 gtcagcgctg ccttgaaagt cccttcttta gccgatactt atacgattat gcgacaagaa
    25621 tatcctcacg ggaaaatcat tgccaacatc ggtgcaggga cttctgtcga aagagccaaa
    25681 gaagctatcc gattgtttca cgcagatgcc ttacagattc atttgaatgc acctcaagag
    25741 ttggttatgc ctgagggaga ccgtgatttt accaattgga aagtacttat tcaagaaact
    25801 caaacagcca ttgacgtccc cctcatcgta aaagaagtcg gttttggcat gacaagagaa
    25861 acgctcaatg atctagctgc tttaggagtt catacagttg atatcagcgg tcgaagcgga
    25921 acaagcttca cccagatcga aaacgcccgt cgctccaagc gggaactgag ttatttagct
    25981 gactgggggc aatcgacagt ctcttcattg cttgaagcaa atgaagcaga cacttctatg
    26041 gaaatcctag cctccggagg tatccgcaat gcctatgaca tatttaaagc cctttgtctc
    26101 ggcgcaaatg ccgtgggtac ttcaggaacc gtactgacgc atctgatgaa tcacggcgta
    26161 gaagaaacta ttatactgat gaagcaatgg caagaagagc ttcgtctgct ttatactatg
    26221 gtcggcgcaa cgaataccgc ggccctacac caacaatctc tgatcttttc cggaccagtg
    26281 aaggattggt gcgaagcaag agggatcgac ttagtgaagt atggaagacg cacagaaaag
    26341 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta caaacggcgg
    26401 aagtaaaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
    26461 cgtgaattcc ttcttatttc ttggagttaa atgcttctgt cccagcctct tttttcgaat
    26521 aaatgatgtt taatcagctg tccttcggta ttcgttcaat ccgctgattt cacaacctct
    26581 ccactaaaca gtgtactaaa gctcttttaa ggttgttttc cgatataggc aagtatgcct
    26641 ccgtccacat acaagacatg gccgttaacg aaatcagatg cgctagaagc caagaagaca
    26701 gatggtccag ccaaatcgat tggctcgccc cagcgtgctg ctggcgtacg tccaacgata
    26761 aattgatcga atggatgacg ttcaccgttt tcttgtgttt cgcgtaatgg agcagtttgc
    26821 ggtgtagcaa tataacctgg tccaattcca ttacattgga tattgtattg accgtattca
    26881 gaagcgatgt ttttcgtcag catttttaat ccgccttttg ccgcagcata agcactgacc
    26941 gtttcgcgtc ctaattcact catcatggag cagatattga tgattttacc gccgcctttt
    27001 tcgatcatat ccggaataac tgcttttgaa acgatgaatg gggcatttaa atccacgtcg
    27061 attacttggc ggaagtctgc cgcatccatt tcgatcatcg gtgtccgttt gatgatccct
    27121 gcattgttga ctagaatatc aactgaacct acatcttctt tgatctgttg aaccatcgct
    27181 tgaaccgctt cttcatcggt cacatcgcag acataacctt ttgcatcgat gcctgcttct
    27241 ttataatgcg ctataccttc atcaactgat tcttgattca agttattaaa tacgattttt
    27301 gctcctgctg tagctaaaga gcgtgcaatc tcaaatccaa taccatagac agcaccggtg
    27361 actaatgcta cttttccatc taaacgaaac atatccatac taaattccat cattttctct
    27421 cccttatgct tattttaatt gatccatttg caccatatcc atgtctgtat aggtgatatt
    27481 ttctccgcac attgcccaga tgaatgagta attgcttgta cctacacctg aatggatcga
    27541 ccagcttggc gaaataatgg cttgttcatt gtctacaact aaatgttttg tttcatctgg
    27601 tttgcccatc atgtggaaca cacgtgtatc ttcattggca taatcaaagt agacataagc
    27661 ttccatacga cgttcatgtg tatgacaagg catcgtattc caagcgcttc ctggttctaa
    27721 aatcgtataa cccatttgta attgacagct ttcgcataca tttggatgga tgtattggta
    27781 gattttacgt tcattcagtg tcaggccttc accagtctcc attggtttta cttggtcgat
    27841 actgattttt acatttggat atttatgatg agctggaaca gaactaatat agaatttagc
    27901 tgggttttca gcatcttgag aagaaaatac aacatgtcgt gtttcttttc cgatatagta
    27961 tccgtcttgt tttttcattt cttctttttg cccatcgatc tcgatgaatc ctggtccacc
    28021 gatattgatc acacctagtt cacgacgctc taagaagtaa tcaacaccta gttctttgtc
    28081 taatgtgatt tctaattctt tttcagtagg tgtcactcca ccaaagatca ttcgatcgtt
    28141 gtgcgtataa gtcaaactga ttttccctgg ttcaaatacc ttttctacta aaaattcttt
    28201 tctcaattgt tccgttgaat aatgacggat atcttctgga ctgtgtgtat aacgtgtttc
    28261 catttcttgc attttcttct actccttcta tcgaatgatt tttccagagc cgcatgctaa
    28321 aaattgtgcg acttcttctt gactaaattg gttgcagtcc ccatggatcg tatgcttcaa
    28381 ggcagaagct gccgtagcaa aatcaatgat ttcttgcgga gacatattgg ataaaatccc
    28441 atggagcacg ccacctgcaa aagcatctcc gccacctact cgatccacga ttggggtgat
    28501 tctatggact tgggattcca catattcatg gttcatccac aatgtcccct tcaattcatt
    28561 ttcacttgct gaatgaacgg tacgcttggt cgaataaaac acctgaatat tcgggaactt
    28621 ttcctgcatt ttttgataat aataaattag aggttgttct tctgcttctg gcgctttctc
    28681 gatacctaac aaataaagag catccatttt tccagctgag cagatatcta ctaaaggtaa
    28741 aagctgatgg attacttcgc tggcttcttg ctgtgtccat aattttccac gatagttgat
    28801 atcgaagctg atcaggcagc cagctttttt tgcagcttct atcacctgtt tcgtcatttt
    28861 ttgccatttt ttagacaaag ctggggtgat tcctgaaaca tgaaatatat ctattccttg
    28921 gaataactcc tctagcgacc attccaaatg atccatttca gcaaaacttg atccggcacg
    28981 atcatagatc actgaggcgg cgcgttcgcc actaccagtt tccatataat aagtacctag
    29041 tcttgatcca ccagataata cctgtttcgt atctactcca taacattgca agtgcttttt
    29101 gactgctttt cccaatgcgt tatcaggtac ttttgtagca aaaatcgctt catgtccgta
    29161 attagctaaa gaaattgcta cgtttgcttc ccctccgcca taatgggcag cgaatcgttc
    29221 acttgaatcg atacgggttc cctgctctgt ggacaatcgc aacatgattt ctccgagtgt
    29281 cactactttt cccataagca gctacctctt gatttctttc atttttgctg catactgttg
    29341 cgcgacttcc gttactttgt caaaatctcc tgtagctgcg ggtgctaaaa ggttcccacc
    29401 tactccgact gttacgacac cagcatcgaa ccactctttc atattgtcca gactcacacc
    29461 gccagttggc atgatattga cttgaggcat cggcgccttg aaggcagaaa tgatacttgg
    29521 tccataagcg cttccaggaa atagtttgac gatatctacc ccgcttttca gcgctgtttt
    29581 gatctcggta atagtcatac atcctggtaa gtaaggaatt tgatacagat tgcacatctc
    29641 tgccgtttct tgatcaaagg aagggctaac aatatactct gcgccagcta agatagccaa
    29701 gcgagctgtg acagcgtcta atactgtgcc tgcgccaatc actatttctg gttggtcttt
    29761 gtagaatgac aaaagttctt tgattacttg atctgcttga gggactgtaa aagtcaactc
    29821 aataccagtt agaccacctt tgatgattgc gtggcaagca ttcaaagctt cttctttgct
    29881 ttttccgcgt accaccgcaa tcactccggc gtttttcaat cgagataaaa tatcaatttt
    29941 tttcatcttc tccacctatt tcctatttag aaattttatt ttataacttg gaaatctata
    30001 cccatattat acggcagaaa agaaaaattg caaacgcttt tttcattatt tttgaattct
    30061 ttgctcgatt tcgctttttg tttgcttgag ggcactgatt atttgttctt ttgtctcatc
    30121 tgttaaacga tatttcggca tacttacact aaatgcacca atgatttgtt cgtttttcac
    30181 gattgaggct cctatacaaa aaatatcacg ttcgatttct tcatcgtcaa atgcgatgtg
    30241 ggtttctttt accgttttga tttccttttt caaccgtaat gcattggtga tcgtatattc
    30301 tgtgtaaggg atcaacggat gcttttcaag ataatcagtg tactgctctt cactgaattc
    30361 tgctaatacg gctttgccca tcgcagaatt gtataatggt cgggtgattc cgatttttga
    30421 agacatgcgg atcgtttgat gttttggatc tagtttgttc acgtacaaga tctcattgtt
    30481 gtccaatacc cctaagtgga tagtctcatc gatcttttgc tgcagctgtt ctaaaaacgg
    30541 taaagtcaac tctaccaaat cgatttgttc aatgttttta ttcgcgtagc gaataagctt
    30601 tgcacctaaa cgatagtttt tctctgaatt tttatttacg tagccgatca tcaacaaagt
    30661 atctagtatt ttcaagactg tagaagaggt cattcccaca ccctttgcga tcgtctgcaa
    30721 agagacatct tggttttccg ctaaatagtc tagtatttta gaggctttga tcaatacagt
    30781 tccataaggc tttttatttt ccatataata ttctcctatt tctttataag aaaaattata
    30841 gcggaaatac catcaattta aaagagatat ccagatataa actaccattt ctctttacag
    30901 gcaatttaat gttgaagatc cgcttcgttt ttacatcaaa ctttgatata aagcaatgat
    30961 ttcttctact gtcgtttctc ttggatttcc tggtgtgcat acatctcgca aagcatcttc
    31021 tgcaattgct ggaatatctg cttcttttac acctaattca gaaatggttt ctgggattcc
    31081 aaccgctttg ctcaaccgat cgatctcacc acaagccgct tctcttacat cttctaaaga
    31141 catttcagct gctcctttaa tccctagaac aagagcgatc tcacggtact tttctccagt
    31201 atattctttg ttatatttca tgacagttgg caaaagtgct gcacacgcta cgccatgagg
    31261 aatgttgtac catgcactta acgggtgagc catcccgtgt accagtccta aaccgacatt
    31321 tgagaagccc attcccgcaa tatactgtcc taatgccatt ttttctcgcc cattttgatc
    31381 gccatctaca gaagcttcca aagaatggcc gatgatttcg attgctttta aatgcagcat
    31441 atcagtcatt tcccaagcgc cttttgtgat aaacccttca atagcatgcg tcatagcatc
    31501 catacctgtt gatgcagcca attttttcgg cattcccatc atcatatcgc tgtcgatgaa
    31561 cgcgacgatt gggatatcat gaggatcaac acaaacaaat tttcggtgat ttacttcatc
    31621 tgtgatgacg tagttgatcg ttacttctgc tgctgttcca gaagtcgttg gtatagctaa
    31681 tataggtaaa catggatttt tcgtatcagc aacaccttct aaactaatga cgtctgaaaa
    31741 ctctggattc gtcaaaataa tcccaatggc ttttgccgta tcgattggcg aacctccacc
    31801 gattgcgatc aaacagtctg cctctgcctc tttaacaaaa gccactccat cttttacatt
    31861 ttggatcgtt ggattaggta cgatcccgtc gtataaagca taagcgattg ctgctttatc
    31921 tagaagttct gttacttttg ttgctatacc gtgctcgatc aatcctttgt cagtaatgac
    31981 agctgctttg ccgaatcctc gctgctgaaa ttcagggacg atcgactcaa tcgctccttt
    32041 tccaaaatac gatgtttcat ttaaaatcat tcgattactc attttctatt cctcctaatt
    32101 tgtattattt ttcacaataa aaacgaaaga accccaagca accggttgcc cagttgcttg
    32161 ttgctacagt tcaaagactg gcatcaattc ccaactgact ggagaggcat ctggatttgt
    32221 ctccatgata tcttccatgt acttccacca tttttgattg atagatgtat ttgccatttt
    32281 ttcccataac ttttcatctt cgatcactaa gtaaccaaat aacaaacttg tactcggatc
    32341 aagatagatc gaatagtcga tgaccccgtg ttcacgaagt gctgtacgca tttctggcca
    32401 aagttcatca tgtctttttt tgtattcttc ttgtttttct gggtaaactt tcatgcaaaa
    32461 agcttttttc accatgtcag acacctgctt tcgtatgaac ttcctccaaa taacctgctt
    32521 ttggagtgac accaaacgca tctgctaaat gccacaggtt ttcgtccgtg attgtttgca
    32581 agattggtcc ttgcgctttg acgtacgtat agacttctgc tgccttttct gctgtctcga
    32641 tcaaaccaaa gacttcatcc atgtcttttc ctgacccgta gattccatgt tgtggccata
    32701 aaaccaatcg agtttctttc attttttcag ctgttgcttc cccaatctca tttgttcccg
    32761 gaaccatcca cggaatgatg gaaattcctt ctggaaaaac aactaagcat tccgtacaca
    32821 tttgccatag cgtacgacta aaggaacgtt cgtctaattc atgcgtaaaa ctcatcgcta
    32881 atagatgact agcatggcaa tgcataacga tccgattatc tgggtctact tctaaacggg
    32941 cgatatgact catgaaatga cttggcaact cgcttgtagg aaggcattcg ttttccagcc
    33001 cccacaaaag ttccagtgtt ttgccatctt cagcaactcg aacgattcca agattctcgg
    33061 caggttcgct cgctacattt ttaaaatatc ttcctgaacc agttacaata aaatagcatc
    33121 ctgctaaacg gctggcatca aaaatcatcg agatgcggcg gatcactttt tcaggatcca
    33181 agaacggtgt gatctcttct tcttttagta aataagaaat attcccgcca tttctttcat
    33241 cccatcccaa gcgataaaga ttcgacgtcg tttcgatcat ttctttcaca taaggcgctt
    33301 gtaaaatatc tatttttttc aatatagcca cactcctatt ttcttaattc caacacgtct
    33361 ttttgatatt cctggacttc ttccagccaa tccaaaccaa ctggaacttg atttttgtaa
    33421 cagaaataat tccatacatc ggcatacggg aaatctttca attcttccgt ataagccatt
    33481 cttgaagtaa agtcaaaagc cagctccatt tctttcaatc gctcaactgg ttccagcata
    33541 gctttcagca gtgctttttg tgtatttctc gtaccgatga cccaagcagc gatacggttg
    33601 atcgttgcat caaagaaatc cagaccgata tttgtttttt ccaacaattc gttacggact
    33661 aattctttag cgatttcctg caattcatca tccatcaaaa ccacatggtc tgaatcccaa
    33721 cgcactggac gggaaacatg caacatgatt cctttgctga ataaagctaa tgaagataat
    33781 ttattggaaa tcacttctgt tggatgaaaa tgtccagcat ctagacaaac aagtttgtca
    33841 cgagtcaacc cgtaacccat ataaaattca tgggaaccga ctgtataagc ttcagcgcct
    33901 aaaccaaata gtttgctttc tactgcatct tgtacatacc gttcatctaa ctcttctgta
    33961 aaaatttcat cgagtgattc cattaaacgt tcccgtgggg taaaacgatc aatcggatta
    34021 tctttcatcc catcaggtac ccaaaagtta ttgatacttg tttgcccagt ttctttccca
    34081 aagtactccg cgattttgcg gcttcttttc ccgtgttcga tccagtattc acgaacttct
    34141 ttatctggat gagccaatgt aaagccgtct ttcatcattg gatgcgaaaa gaaagtcgga
    34201 ttgaagtcaa gaccgatttc ttcttttttt gcccactcta cccatttttc aaaatgtttc
    34261 ggctcaattt catttagatc gacagattct tctgtatcca aatagatagc atgaaggttc
    34321 aatttgtgtt tgcccggtat caacgaataa gccttctcca gatcttgtct aagctcttca
    34381 ggtgttcgtg cggctcctgg aaaattccct gtcgccatga ttccaccggt caattcgcca
    34441 tccggtgtca agaaaccttt gacatcatct ccttgccagc aatgcataga aattttgatg
    34501 tcctgtatct tttctagtac agcatctgta tctacaccga tagttgcata tttttctttt
    34561 gcttgttcgt aatttctttc aatcgtgttc atacgattac tcctttgatt ttattcacgt
    34621 accattccat cgtgttttgc ttattttcat ctgggtaaaa agtttcacat ggaaacgact
    34681 gtttgattac atttcttgct tgttctatat tcgaaaactg acctgtactg agcatttgca
    34741 tggcaacatt cccgattgct gtggcttcac taggtcctgc ttctacaatg attccggcat
    34801 aatctgctgt caattgattc agtagacgat tattcgatcc gccccctacg atgtgcagct
    34861 tttccaattt cgttccagtt aagtgttcta ctttgatcag ctcttgtgcg taacaaagag
    34921 caagattatc atacacacaa cgagcgatag ctcccgatga gacagggact ggctgctctg
    34981 tttcaaaaca gtattgctga atctcttcta tcatatttgg gggattcaaa aatcgagggt
    35041 cattgatttc catcacttgc tgcagcggtt tttcttttgc agcaagctct gccaattccg
    35101 cataactgta ctcgtactct tgcattcggg caacttcctg gatcaaccac atccccatga
    35161 tgtttttcaa aaaacgcacc gtgttatggg caccccattc attcgtataa ttttcagaaa
    35221 aagcattgcg agaaacattg gcgatctttg tttcaatccc taaaagcgac catgtcccac
    35281 tgctgatata accccaacgt tctccttgac ctggcgttcc taatacagct gacgccgtat
    35341 catgacttgc tactgtgata aaggtcacat ctggcaaatc atattttgaa aattgttctt
    35401 tttgcagatc tcctagtatc gttcccggct ctgtcaatgg tggaaataat tccttcgaga
    35461 caccgatttt ttcaagaagt tctatttccc attcttttgt ccctgcattc agcagctgca
    35521 tagtagatgc attagttttc tccgtcacca tgttaccagt aaagacatac cctaaataat
    35581 caggtatcag taacaattta tgtgcttttg ccaaattttc tttttcttca gcaaacaatt
    35641 ggaacaaggt attgaaaggc tggatctgaa tccctgtctg ctcatacaac ttatctagag
    35701 aatagatttc agaaaactgt tctactgctc cattggtacg cttgtcccga taagcaatcg
    35761 gatcatttat ccgatcccct tgttcatcaa tcagacagta atcgactgcc catgtatcga
    35821 tccccgcaaa aaggcgatca atccctgtct gctttaattt ttcaaatcca atcaaaatct
    35881 ctttgataag ataatcgatc ttccagcgat cgtgaccgtc tttgttccga aagccgtttt
    35941 tgaaccggtg tacttcttcc aagatcaaag aaccttttgc atccctcgta cttttcatta
    36001 gtcgtccgct tgatgcaccg atatcgatgg caacaaatgt tggattcatt tctccacctc
    36061 ttttacttta ttgttgatta gcactttatt aataaagcgt tatcatttat tgacaatgct
    36121 atcataatac agaatcttat attttgcttc acactatttt ggtatataat tgcattatct
    36181 tgacaagttg ggagtgttta catggattac tgggaaaaag ccaaccactc atggtcggaa
    36241 gactcaaaaa gagtgatctt gacgcctaca caaagaagca ggaatttatt tttttatatc
    36301 caagaaatcg gttacttcaa agcaccaatc ccttactata ctgaacgagc acatttacct
    36361 tcttacttga taaagtttac tttgtctggc aaaggttcgc ttacatataa tgataatcag
    36421 caacagctaa aagctggaga cttatttttt attgactgcc aagaatatca gcgttatcaa
    36481 acgatcagcg aagaaccatg ggaaatggat tggatccatt tgaatggtca gaacgtccaa
    36541 tcattttatc aggagttcac aaaagacggc cataacacct tccattcaac aggagatcca
    36601 tcagaaaaca agatccacca tttgatcaca gaattgatcc acatgcaaca aggcacgaat
    36661 gcccgtaccg atttccagtc ctctgtagct atccacgagt tgctgaatga actgattttg
    36721 caaaagtatc agctagattt cgaagaagag gatattcccg cctacgttta caaactgaag
    36781 caatacttag acgaacattt caaagaaacg attacacttg atcaattaga acaacaattc
    36841 ttattgaata aatatcagct gagcaaagat ttttccaaat ttatcggttc tccaccaatc
    36901 gattatttga tcagcaaaaa actttcttac gctaaagacc ttcttcggta tacagaattt
    36961 acggttcgaa tgatttccga agaaataggg attgagaatt ttgcttactt tagccgcctg
    37021 ttcaaagcaa aaacaggact atcgccgaga gactttcgta aattcagctg attcatataa
    37081 tatgtacgtc attttttcaa aatattttga aatatataga taaaaataaa ataaccgcag
    37141 tttctgcttc tatgtaaaca acagcagcta ctgcggttat tttttagtga tcattctcat
    37201 tgttcatctt caaaatgttc ataatgaacc aactgtttct gtgtatcacg tttcaaaaat
    37261 tctttaggcg ttatgccgaa ctctctcttg aatgctttca gaaaatgcgg ataagaagaa
    37321 aaaccacttt tactatatac agcactagag ctgcggtatt ctcgcaacaa gtgcttggca
    37381 tagatcagct ttttcttcat tacatactga tgaaacgtaa atccagtgta ctttttaaat
    37441 tctcgtgtga tatggtattt gctgacaaaa aactgctttt ccatttcctc taaagaaagc
    37501 ggatgagata aattttttga gatatattgt aacatttgtt ccacacgctc attaggaact
    37561 acagcattct gttcttgcgc actctcttct ttttgtacaa cttgattgag aaaaatcaag
    37621 tattctacca gagcttgttc atattttatg tcttcaccgt aaattttttt atcaggagga
    37681 tggtcgataa atgataattt ttgcttcaat acttcctgtt ctatttgcaa tacacgactc
    37741 ctcttctctc ccattggttg aaaacaagcc gatagattag accatggcgt ggaacgactt
    37801 tctagaaaag aaggggtgac aaagatatag acacgctcga atacatctga agattggcgg
    37861 acgatacgat gcaaatcatt ggaatggatg agcagcacac ttcctgcttt gatcgtaaat
    37921 tgtcgtccgt tcaaataaaa agtcgcttct ccctttaatg tagcatggat ctcatagaaa
    37981 tcatgatgat gatagatcgt tttattttgg ctgacgacat ctttgacatg atagatttca
    38041 tactgatcag aaaccatcgt attgacttgt tcaccgctga ttttcttttg gagcatgtgc
    38101 cgcacctcct tttgttcact tatgcacaat tttattatac cacgtttttt gtaatcaaaa
    38161 agagctgctt ttatatactc ttataaaaaa gaagagacgt ggcatcttct agccccgccc
    38221 cctccattct aaatattagt taaccaacgt cttctttaca ttattcaaga agacttcttc
    38281 cataacgaat cccatcatct tttgtttctt caagaaccac atacaggtac ggacgattct
    38341 tcatgatgat ttctttgatt tttgtgtact cgatcacacc gtctcccaaa tttaccggca
    38401 cgattttttc ctgttcgaca cgaaaatctt tcaaatgaac cgcacatatc ttttcaccaa
    38461 aacgttcaaa agcttcttct accaattgga cttgttcttg ataatcacta cttgtgatca
    38521 aattggtcgg atctaaaatg attcctagat agtccgaatc tactgcttgg atcagctgct
    38581 ctacccttgc taaagaatac accggatgat tcaaacctgg ttctatcccc accatcattt
    38641 ggtatttttc tccagctttt accaagcgac ggataacgga cacagcttca gcaaatgctt
    38701 catctgtgaa attttcttcc gtatattgga tttccggcaa aacacatcca gtttccgaag
    38761 caaccatcga cgcaccaaag gaagaagcat agcgaagata ctgctcgaac tttgtcagca
    38821 actcttgtcg tatcgttaaa tctggatgaa tcatattgat atagcaactt aaaattccta
    38881 cggtcacttc ttgcttttct aaaactcgtt tgacataatt tcccatccca gaattgatct
    38941 catttgcgcc agaaggtatt tcaggaaacg aaagacctaa ggcgagctgc agtgtatgaa
    39001 tcccttgttc ttttgttttt tctgcaagat cttctatcgt ttggacttcg gaaagatcat
    39061 gtcctctaat tgtaatgttc aacataacta ttctcctaac tcagcatatt gggtagccac
    39121 atgacgattt gcgggaagta agtaatcaaa aacaagacga ctaagataac cccataaaaa
    39181 ggaagaagcg gtttcagcat tgcctctgct tttacgcctc ctacacttgc accgacatat
    39241 agtcctgtac ctactggcgg cgtgatggag cctacacaca gattcatgat tgaaaacaac
    39301 ccgtaatgta caggatcaac ccctacactt gtgacgattg gtaaaaatat tggtgtaaaa
    39361 atcaagatag caggcgccac atccataaac atacccacca gtaataacac aagattgact
    39421 aataacagaa taataaattt attatccgtt acgcccataa tcaacgaact aattgcctga
    39481 ggaataccgg taaacgccat ggcgaaagac atcatggaag aagtagccaa taagaacatg
    39541 atcgtccctg acattgctac tgattgaaac aacatattag gtatatcttt tagttttacc
    39601 gttttatagt aaaacatagc aatcaacagg gagtaaatga cagctatcgc tgaagcttct
    39661 actgctgtaa atatccccgt caagatacca ccgataatga tgacaatcaa tagaaggcta
    39721 ggaatcgcct cttttattat ttttccagca tgagccatct gtcctttttc gacaacaggg
    39781 tatttattct ttttagcaac gatgaatgct actagcatga ttcctagcgc ccacaaagcg
    39841 cctacgagat aacctcccat aaacaaagca gagatcgaag caccaccagc aacaagtgaa
    39901 tacatgataa aagcagtact tggaggaatg accatacctg tcggagctga cgcgatattg
    39961 accgctgtcg caaacttccg gtcatatcct tcttccactt gctgagggat caacactccg
    40021 ccaatcgcgg tagaagcggc aatggccgag ctagagatgg aaccaaataa agcattccct
    40081 aacacattcg tatgagccaa agcccccggc acgcgcccga cgaacaacat cgcaaaatcc
    40141 actagtttct tagctatccc tccattattc atgataatgc ccgagagaac gaaaaacgga
    40201 atagcgatca gcgaaaaact attgatactt cccaccattt tttgagctga actaaaagca
    40261 gaaacatcaa acggtataac taacaataaa gtcatcatcg atgagaatgc aatacttata
    40321 gcaatcggca tccctaatac aagcaacaca acaaatacaa aaaataacac taatccggct
    40381 tctattgcca ttttattcct ccatttttat attttttctt gaattgctgg cttgttgctt
    40441 aatgtatcga ttagattgat caggctatag ataatgatga gtacccccga tacaggtact
    40501 gacagataaa catatcccat aggaatactt aaagaaggcg agatctgccc cattgtcaat
    40561 gtgacagcat tccatccgcc atacaccatg atcatcgcag caaataccag aaataaactt
    40621 tgaacaacaa cttctaaaat tttacttttt ttgccagtta atttttcact taaaaccgtt
    40681 acggctaaat gtgctttttg tcccacaaca tacgcggaag acaacatggc aaaccagaca
    40741 agaccgaatc gaacaagctc ttccgtgacc gtatttggat ttcctaaaac cgttctggaa
    40801 aatacttgcc ataaaacaac agcgaccatc ccaacaagca aaacagctcc caggatctct
    40861 aataccttgt ttaaaatcat tttcatcaca gctacgctcc ttgctcttgg atcttttgat
    40921 aaaggtcttt gtaggtagga tcgtttttga atatatcatg aagtggggca actgcctctc
    40981 gaaacggtgt attatctaca tcattgaatt ggactccatg ttcttcttgt gctaaagctt
    41041 tttcttcttc aattgctttt gcaaaagctt caatttcaaa aaccgttgat tctttcgctg
    41101 cttcttcaat ggctttttcc tgctcatcag ttagctgttc tttgatggca tcattcatga
    41161 tgatgatatc tggcacacgt gtatgctcat cataggaata gtatttagcc acaccaccat
    41221 gtccagctgt atacaagaca aattcattgt tttctgatcc gttgatcaaa ttggattgaa
    41281 gagacgtgta cacttcatca cttcccatag gtacgggcga accgcctaaa agctctacca
    41341 ttttgatagc ggtttcgctt tgcatcacac ggatcttctt tcccttcaaa tcttccggcg
    41401 tatggatcgg accgtctacc atataaaagc tgcgctgacc agaatcataa taggtcaatc
    41461 cggtaaatcc taaatttttg gtcgattggt agattggctc cattatttct tcattatcca
    41521 tcaccttaaa gaaatgttct tcatcattga atagataagg aactgaaaaa atcgaataat
    41581 ctttagagaa actttccaac gctgaggcac ttacttttgt gaaatctact gctcctgact
    41641 gtgtcaattc gatcagttca cgttctccac ccagttgtcc atctgggaaa tactggatct
    41701 gtacggaacc atctgttttt tcttttacta aacgcccaaa ttcattcatt gcatcaatta
    41761 ctggctggct gttgcttgca taagcaaagc gcaaggtaac tatctcattt acatctgcat
    41821 ctgcgacagt tgccacacta gaattatttc cgcagccgct tagacctagc agacctaggc
    41881 taagcgaact tgctgcaaac agcagtgatg tttttttcat atgggtttcc tttcttttaa
    41941 caataagaga cgagcaattc tgtcaaacaa agcaccgtta acgactgtcc ataaggcatt
    42001 gctgtgattc cgatattccg ataaaaatct aaactgtccc ccattcctgt cccgattgaa
    42061 acgttttgaa cttctccttt ttcatcgatt tgttcaagca atccttggat agcacgataa
    42121 gcgacttctt tatattcttg tggtaaataa cgtttatgga ccgctttcaa aataccgtaa
    42181 gcaaatcctg cagttgcaga agattctacg taagaagaag gatcatccaa caacgtatgc
    42241 cataaaccgc tttcagactg ataagttttt aatgcacgca cttgtgcttc aagtgtttcg
    42301 atcaataatt tgcgcaatgc atcttctttc gataattcta agatttcaat gatctctgga
    42361 atagcaatcg tcaaccagca gtttccacga gcccaaaatg cttcggcata gttatgattt
    42421 ccttcaaacg tccagccgtg ataccacaat cctgttcgtt tatccatcaa gtatttgata
    42481 tggatcataa actggtaacg agcttcttct aaatattctg ggcgattcaa cagtttcccg
    42541 atttttgcca aaggaagcac tgtcatcatc aatgtatcgt cccacaactg attcttattt
    42601 tccggtccat atgtggcatg ctgtaacccg tcttcatttg tccgaggcat gtcattcatg
    42661 acccattctg cccattgttc taaataaggc aaatagcgtg aatctttcgt gtcttcatac
    42721 aagtaagcca tagtcaatag cggtgccatt gtattgacgt ttttgggcgg agcgccttct
    42781 tccatacgat cctcaaacca ttcattgacc acttgataag cgcgaggatt ttttgtcatc
    42841 tggtaatttt tataaatacc atatagaccg acaccttgtg gccaattcca cactgtccag
    42901 cttttgtcat ctactttcag tccatcaaag tccaacaaaa attcaccatt atcgtctttg
    42961 atcgttgtca ggttttcaac gagcaggtca attttttctt tcactaattc tttaggtaca
    43021 aaaatctctt ctttaataat catctttacg ccaccttttt aaacgctttc attttatatc
    43081 cttaatatag cagtaatacc gtccactata aatatcgctt tttgaacaaa aactgtctaa
    43141 aattgaccta aatgttaaat aacaaaagta actttcagtt acttggaaat taaaaaaaga
    43201 acttggcgaa acaacgtttt ccaagttctt tttcatctat tttcttatct tttttctacg
    43261 ctttcttttg cttatccgtt gtaagcatca atgatgatct gtttcaattc agagatcaat
    43321 ggttctttcg gattagctgt cgtacattgg tcttcgtaag ctaattcagc catacggtct
    43381 acggttgtat ctagtgtttc ttgtgtgact ccttgagctt ttagactcat gttgatgccg
    43441 acagattttc ctaattcata aacagccgtt gctaaagctt cgactaattc ctctgttgtt
    43501 tcgcctttca gacctaagaa ttttgcgata tctgcgtaat ctgtatctgc acggaaatag
    43561 tcatatttag ggaacatcgc atgtttttgt ggatcttttg cattatagcg gataatatgc
    43621 ggtaaaagaa tcgcatttgt ccgtccatga gggatcccgt attccccgcc gattttatgt
    43681 gcgactgagt gacaaatacc taggaatgcg ttagcaaacg ccataccagc cattgtagaa
    43741 gcgttgtgca ttttttcacg tgattccata tcaggtgttt ttactgattt ttctaaatag
    43801 tcaaagacta acttgattgc ttgtaggctc aatccacgtg tataatctga agccatgact
    43861 gaaacatagg attcgattgc atgtgttaac acatccatcc cggtatctgc tgtgacagat
    43921 gctggaactg acatgacgaa ttgaggatcg acgattgcga catctggcgt caatgcataa
    43981 tctgctaacg gatatttcac atgtgtttca ctgtcagtga tgaccgcaaa tggcgttact
    44041 tcagaaccag tccctgatgt tgtcgggata catacgaatt gtgttttttc tggtttgtcg
    44101 atcttatatg tccgtttacg gatatccaag aatttttgtt ttgcaccaaa gaaagatgtg
    44161 tctgggtgtt catagaacat ccacattccc ttagctgcgt ccattgcaga accgcctcca
    44221 agagcgatta ctgtatctgg cttgaagtct gccatcagtt tcgttcctgc gtatacggta
    44281 tttgtcgatg ggttcggttc aacatctgag aagacttcga tcttcacatc atttttacgt
    44341 cgtgccaata cttcacgaac tgtatcgcag taaccgaatt ggaccatacc agggtcacaa
    44401 acgatcatga cacgttcaac gttttccatt ttctctaagt acaatagaga gttcttttca
    44461 aagaagattt ttggcggtaa tttgaaccat tgcatgttat ttctccgttt cgctacagtt
    44521 ttgacattga ttaagtttac agcagagacg ttacgagaaa cagagttctt accataagaa
    44581 ccgcatccca aagtcaatga cggaatcatt tcgttataga tatcaccgat gccgccttct
    44641 gcagatggtg tattcactaa aatacgacaa gctttcattc gaagaccgaa tttgacatgc
    44701 aactcttcgt tttctgaatg gataactgct gtatgtccta atcctccaag attcaacata
    44761 ccttcacaca aatcaaacgc atggtccgtg ttgttagctt tgatcatggc aagaactgga
    44821 gaaagttttt ctcttgatag cggataatct gggcctacgc cttccaattc agcaatcagc
    44881 attttcgttc cttttggtac actgattcct gccaattttg cgatttccat cgcagaatgt
    44941 ccaacgattg atggattgac cgcatatttt ccttcgttca tcactgcatc ttctaatttt
    45001 tgcaattcat ctggttttac gatataaact tggtgtgctt ggaattctgc ttttactgct
    45061 gcatagattt ctttatccac gatcacagct tgttcagaag cacaaatcat tccattatca
    45121 aatgttttcg aaacgatcaa atcgttcact gcacgtttaa tttttgctgt tttttcaata
    45181 taagctggta cgttacctgg acctacacct aaagctggtt ttcctgtaga gtaagcagac
    45241 ttgaccattc cagctccacc tgtcgctaag acgatcgcga tacctggatg gttcattagc
    45301 atagaagtcg cttcgatcga aggatgttcg atccattgga tacaattttc aggagctccg
    45361 gcagcgatag cagcatcacg taccactcgt gctgcttctg cagaagattt ttgggcactt
    45421 ggatggaaag caaaaacgat tggatttctt gttttcaacg cgatcattga tttgaaaatc
    45481 gttgtggaag tcggattcgt tgttggagtt actccgcaaa ccacaccgac tggttcagca
    45541 atttcaatca accctttttg gacatcttca ttgatcacac caactgtttt atcatgtttg
    45601 atattatgcc aaatatattc ggacgcatac atatttttga ttgctttatc ttcgtaaaca
    45661 ccgcggcctg tttcctctac agccatcttc gctaatggca tatgttgatc caaagctgcc
    45721 attgccattt catgcacgat atggtctact ttttcttgat caaagctttc catttctttc
    45781 aatgctgcat ttgctttttc tgccaaatca tcaatcattg cttgtacgtc aattgtcttc
    45841 gttttttctt tttctattac tttagccatc attatcctcc taatgggtca tggttatttg
    45901 ttaaataatt cacatgtata tcatacaata tttatttttc tatgtaaaca cttttttgtg
    45961 atttttttca cagttatttt taaatataaa attgc
//
LOCUS       DAZUKC010000015        43871 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000015 DAZUKC010000000
VERSION     DAZUKC010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.015"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            566..868
                     /locus_tag="ACG80T_000763"
     CDS             566..868
                     /locus_tag="ACG80T_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4840435.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNTFKIT"
     gene            940..2166
                     /locus_tag="ACG80T_000764"
     CDS             940..2166
                     /locus_tag="ACG80T_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4840436.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(2254..2646)
                     /gene="rpsI"
                     /locus_tag="ACG80T_000765"
     CDS             complement(2254..2646)
                     /gene="rpsI"
                     /locus_tag="ACG80T_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFS4840437.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(2662..3090)
                     /gene="rplM"
                     /locus_tag="ACG80T_000766"
     CDS             complement(2662..3090)
                     /gene="rplM"
                     /locus_tag="ACG80T_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFS4840438.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(3624..4091)
                     /locus_tag="ACG80T_000767"
     CDS             complement(3624..4091)
                     /locus_tag="ACG80T_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFS4840439.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(4211..5749)
                     /locus_tag="ACG80T_000768"
     CDS             complement(4211..5749)
                     /locus_tag="ACG80T_000768"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFS4840440.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            5829..6515
                     /locus_tag="ACG80T_000769"
     CDS             5829..6515
                     /locus_tag="ACG80T_000769"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFS4840441.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(6716..7243)
                     /gene="msrA"
                     /locus_tag="ACG80T_000770"
     CDS             complement(6716..7243)
                     /gene="msrA"
                     /locus_tag="ACG80T_000770"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS4840442.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(7327..8004)
                     /gene="pgmB"
                     /locus_tag="ACG80T_000771"
     CDS             complement(7327..8004)
                     /gene="pgmB"
                     /locus_tag="ACG80T_000771"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFS4840443.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(8001..9131)
                     /locus_tag="ACG80T_000772"
     CDS             complement(8001..9131)
                     /locus_tag="ACG80T_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFS4840444.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(9131..11314)
                     /locus_tag="ACG80T_000773"
     CDS             complement(9131..11314)
                     /locus_tag="ACG80T_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFS4840445.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(11311..12336)
                     /locus_tag="ACG80T_000774"
     CDS             complement(11311..12336)
                     /locus_tag="ACG80T_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4840446.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(12381..13208)
                     /locus_tag="ACG80T_000775"
     CDS             complement(12381..13208)
                     /locus_tag="ACG80T_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS4840447.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(13205..14272)
                     /locus_tag="ACG80T_000776"
     CDS             complement(13205..14272)
                     /locus_tag="ACG80T_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFS4840448.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(14298..15134)
                     /locus_tag="ACG80T_000777"
     CDS             complement(14298..15134)
                     /locus_tag="ACG80T_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4840449.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(15131..16000)
                     /locus_tag="ACG80T_000778"
     CDS             complement(15131..16000)
                     /locus_tag="ACG80T_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4840450.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(16070..17353)
                     /locus_tag="ACG80T_000779"
     CDS             complement(16070..17353)
                     /locus_tag="ACG80T_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4840451.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(17367..19052)
                     /locus_tag="ACG80T_000780"
     CDS             complement(17367..19052)
                     /locus_tag="ACG80T_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFS4840452.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            19327..20352
                     /locus_tag="ACG80T_000781"
     CDS             19327..20352
                     /locus_tag="ACG80T_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4840453.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(20947..24600)
                     /gene="rpoC"
                     /locus_tag="ACG80T_000782"
     CDS             complement(20947..24600)
                     /gene="rpoC"
                     /locus_tag="ACG80T_000782"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFS4840454.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVKETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(24679..28296)
                     /gene="rpoB"
                     /locus_tag="ACG80T_000783"
     CDS             complement(24679..28296)
                     /gene="rpoB"
                     /locus_tag="ACG80T_000783"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFS4840455.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLFALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            29024..29992
                     /locus_tag="ACG80T_000784"
     CDS             29024..29992
                     /locus_tag="ACG80T_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4840456.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            30010..30915
                     /locus_tag="ACG80T_000785"
     CDS             30010..30915
                     /locus_tag="ACG80T_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4840457.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGSAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            30912..31718
                     /locus_tag="ACG80T_000786"
     CDS             30912..31718
                     /locus_tag="ACG80T_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4840458.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            31875..32843
                     /locus_tag="ACG80T_000787"
     CDS             31875..32843
                     /locus_tag="ACG80T_000787"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFS4840459.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(33160..33684)
                     /locus_tag="ACG80T_000788"
     CDS             complement(33160..33684)
                     /locus_tag="ACG80T_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFS4840460.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            33971..35782
                     /gene="scm"
                     /locus_tag="ACG80T_000789"
     CDS             33971..35782
                     /gene="scm"
                     /locus_tag="ACG80T_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFS4840461.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(35937..36923)
                     /locus_tag="ACG80T_000790"
     CDS             complement(35937..36923)
                     /locus_tag="ACG80T_000790"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFS4840462.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(36946..37512)
                     /locus_tag="ACG80T_000791"
     CDS             complement(36946..37512)
                     /locus_tag="ACG80T_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFS4840463.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(37526..37942)
                     /locus_tag="ACG80T_000792"
     CDS             complement(37526..37942)
                     /locus_tag="ACG80T_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFS4840464.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(38399..40333)
                     /locus_tag="ACG80T_000793"
     CDS             complement(38399..40333)
                     /locus_tag="ACG80T_000793"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS4840465.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            40635..41570
                     /gene="pplA"
                     /locus_tag="ACG80T_000794"
     CDS             40635..41570
                     /gene="pplA"
                     /locus_tag="ACG80T_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFS4840466.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            41660..42763
                     /locus_tag="ACG80T_000795"
     CDS             41660..42763
                     /locus_tag="ACG80T_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFS4840467.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            42776..43729
                     /gene="menA"
                     /locus_tag="ACG80T_000796"
     CDS             42776..43729
                     /gene="menA"
                     /locus_tag="ACG80T_000796"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFS4840468.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
BASE COUNT        12810 a         9205 c         8234 g        13622 t
ORIGIN      
        1 ctttttcact tctaacataa ctttctcctt ctaaatgata atgattatca cgatcatatg
       61 gtatacaaaa attatatttt agtcaaccct aaattttata gtttagattt ttttctgttg
      121 ctgaagatta gaatgacaaa actatttgaa actacgcgta tagtaagtag ctttcaaaat
      181 atataaaaaa ggagcgttct caaatgagaa catgctccta aaactacttt attggcgtat
      241 ggaaaaatta tacttgaagt ggatagcggg ttgaataagt tgataagcat caaaacattt
      301 ataaaaatta ttcaagacta tgtctgttcc atatacaaat atgaaaatca gtaaactcca
      361 tttaactttt ttcgtagtgt gtctttttat acatgtttat cgttattaat agtgaatagg
      421 aaaaaaacta tttttgatca ttgacaatta aatacaattt ttgaataacg atccctgcta
      481 ttcgagctag aaactcaagt acgccatgac ctgattagcg acaaatactt agagatttct
      541 ttttgttctg ggaaaacaaa atgccatgac aatagcagca aataatgata cttccgtaag
      601 gctcgccgta aactggggag aggtgaaatt cctatgtgct tacccaaagc aattcaaaaa
      661 tttcataact actattttag gagatgattt tatagaatcc agagtctacc tgaataaaaa
      721 acaaacctgt gcatttctag gaatcgataa taatactcta gataagtgga ttcgtatata
      781 taatttccca gtaataagag ttggtggtgt agttcgcttt gacgtagaag atattaaaga
      841 ctttatgaat acatttaaaa taacttgaaa ttttcttctc gccgcatata ctaaaattag
      901 tgaaatatac tgccttactt tttttaagga gtgaggattt tgcctacaaa gctttcaaat
      961 gggaaatata aaactaatct gagatatcca aaaaggttta aagaaataac aggaattgca
     1021 tcagaaaagt atcaaaaaac atttccaaat aggcaattgg cgattaaagc cgaaaatgat
     1081 atgaagaaga aaatagaaaa agttttgaga gaggagaatg caaactcatt agaattaaaa
     1141 ggaaaaataa cgtttaaaaa attttacgaa tctaaatggc ttcctagata tgaattaggt
     1201 caaacaatta gatcaaacag gcctcctagt gatataacca ttagtaatac aaaagatata
     1261 tttcggttgc atattttacc tatgtttggt gaatacgcaa tgaattatct aaatatcaat
     1321 actgagataa tatcagatga attaaccaaa aaatcgaaag aatatgcaaa tataaaaatc
     1381 ataaaaggat atgttcgttc catgttcgat attgcagaaa tactaaatta tatcgaattt
     1441 aatcgaacta ctaaaattat tcaaagtatc actgcaccta aaaaaaatgc tttagaagaa
     1501 aaaagaatac aagaaggtaa gcaagcacta tcatcaaaag aacttactaa ttggatagaa
     1561 gcagtaaatg atgattttaa taatcattta cttacttttc atgattacac actttttatg
     1621 attaccctgt atcttggtga tagaaaaagt gagacttacg ctttgcaatg gaaatatatt
     1681 gattttgaaa aacaaacggt ccgtcttaaa catactttag ataaatatca aagaaaaaag
     1741 tttacaaaag gtcgaaaaga taccgtcatt caagtaccag aagttgtaat gacattgcta
     1801 agtgaatgga aatcggtaca agctgatcaa ttgttgaaat taaaaattaa gcaaacgtta
     1861 gatcagtatc tctttactta tactaaacca tctggagaag taaattgtcc tgttcatgcc
     1921 gattacctaa actatcgaat caattcaatt aaaagaaggc atcctgactt agttcattta
     1981 tctccacaca aactccgtca tacttatgca actatagcta gacaaggtgg agcagatatg
     2041 aatcaaattt caaatgcttt aacacactca gatatttcta caacaaagat atatgtgaac
     2101 acaccagata tagttgataa ggctgttttt gaagcttttc agagaggatt gaataagtgt
     2161 gattgatatg tgttcttaaa tgtgttctta aaaacaaaag aagcactttc caaataattg
     2221 aaaagtgcca gaaacgttga tatataaagg atattaacgt tttgagaatt gtgaagcttt
     2281 acgtgctttc ttaagacctg gttttttacg ttcgaccata cgtgcgtcac gagtaagtaa
     2341 gccagcacgt ttaagagcta aacggaagtc aggatcaact tctagcaatg cacgagcgat
     2401 accatgacgg atagctcctg attgtccagc gtatcctccg ccgtttacgt taacaaatac
     2461 gtcatatgcg ccttttgttt cagtaacagc gaaaggttga ttgattactt cacgcaagtc
     2521 agcatgtgga atgtattctt caacatcttt tttatttaca gtaattttgc cagttcctgg
     2581 tactagacgt acgcgagcaa ctgcgttttt acgacggcct gtgccgatat attgaacttg
     2641 tgccaatgaa attccctcct attagattaa gtttgtgatg tctaaaactt ctggttgttg
     2701 tgctgcatgt ggatgttctg ctccaccgta tacgttcaat tttgtaaatt gtttgcggcc
     2761 taaagtgttt ttaggaagca taccttttac agaagtttcg atcaaacgac gagaattttt
     2821 agcacgcaat tcgccagcag tgattgattt caatcctcct gggtagttgc tgtgacggta
     2881 ataaattttg tcagtcgctt ttttacctgt taatttcact ttgtctgcat tgatgacgat
     2941 gacgaaatcg ccagtatcca aatgaggtgt gaaagttggt ttatttttac cacgtagtac
     3001 agatgcaaca actgttgaaa gacgtcccaa tggaacatca gttgcgtcta ctacatacca
     3061 tttacgttct acttcgcctg gtttggccat atatgttgta cgcacgttat tttcctccaa
     3121 tttaagttct gatgtttgac atacacttcg agtttccggg gctctttgtg gggcaaacaa
     3181 taccatttaa catattaccc gctaatctag tcaatgtcaa cgtttttctg cttgattgaa
     3241 aaatttatat gaaactttac ttcttttctt tcttttatat aaaagggtcg tttcctgtca
     3301 aaatgaaaaa caaaaagagg ctgtgacaaa agtcttgtca caacccctta ttttgaataa
     3361 acggtgttct cctgccaact cctcggtcac aagtcacaat atccataaaa cataagaagc
     3421 tgttttttaa aattgtttct tgtgactcgg agctgaacgg caggcttaca acctcttggt
     3481 aaacggtgtt caatcagctg accctcggta ttagctcaaa aaacggcaaa atatgaggaa
     3541 ctattttgac gtttgtttct ctaatactca ggtcaaaacg ctgatttcac aacctctttt
     3601 tcggtcaagt tcaacaacag tttttacgcg tcgatctctg gcgctttgcc caaatgagct
     3661 tggatcatcc agatacgttt ttctgtggct gttttgaatt cgataaagat atcttgtgtt
     3721 ggatcgtcgc cttcttctcc agaaacttcg attccttttt ggtaaagatc tgctagataa
     3781 cgatagcctg caacaagtgt ttccaaacgt tctggaatag tacgatccca gcggccaatt
     3841 tcatcaggga tttttgtgtt atctgcaaat tcacgcaatg tagaataagg tgaaccgtct
     3901 aatgtgatca agcgttcgga tacttcatct aactgagcgt tcaaatcatc catgaactca
     3961 tccatcattg ggtgcaatgt aaggaaacct ggtcctctca tgtaccaatg tgtttggtga
     4021 acaacaacag acatttggct tagatctgca actaattgat ttaatacttc tttggtttgt
     4081 tggaatttca tctttcttgc ctcctaacat tttcttacac ttaaaataat agtagacttt
     4141 caatgaaaag tacaacaaaa tcatttacct gagaacgatt ttcagttcgc ttttttatcc
     4201 gtaaagccaa tcatttttca gaatcagcta attcttctcg cagttgagca aattgctgat
     4261 aagtctgtgc taaatgcttt tgtgtcgaaa caaataatgg atagattttc tgatattttt
     4321 tatgatcagt ttcttgtact ccaacttgct ctttcaattc tatcaattcc tgtgcttcac
     4381 tgatactttg gatctttccg atgcttctcc agcctaaaat cacagctcct aatgcggatg
     4441 cttctgtgac agtagggaaa acaagcgggt aacccagtac atcagccatc atctgacgaa
     4501 aaatcggaga ttgagcaaac ccgcctgtag cacggatctc tgtgagctga ttttcctggc
     4561 aaactccttt taaaatccga taaaggttga gacagatccc ttctagaatc gccttcatca
     4621 tttctttttc tgtatgttgc cgctgtaaac cgataaaact gccaaacgca tctgcgtccc
     4681 aaagcggtgc tcgctcacca agtaaatagg gatgaaaaat cagaccattg gcacccgctg
     4741 gcacaaaagc gatagccgaa agcagtgcat catatggatt ttctcctttt tctttagcag
     4801 attgaacgac ttcctgtaaa aatgtatgcc ctgcccaatc aaagatgcca gcaccattgc
     4861 tagtcgctcc tcccactacc caatgtttct gatccagaac ataacaaaac gtctccattt
     4921 ctggatggaa ctgaggctga gtagtaataa aacgaagtgc tccgctcgtt cctacggtca
     4981 aagcggcgat cgtatcacca aaagcaccta accctagatt agaaagcggc ccgtctgccc
     5041 ctcccaaaac aaatgatata tcatttggca aacctagttc tgctgctgtt tttgatggta
     5101 gacctgttac ttgataggtg gaaggaaaga cttgcggcaa ttgctctatt cgaatgttca
     5161 agtagtcaag cacttcttct gcccattgga attcatggat atcaaaatat ccagttcctg
     5221 aagctgttcc gtagtcgctg atcagttcgc caaacaatct ataaaagata tagtctttga
     5281 taccgacaaa ataagcagtt tttttgaata attcaggttg gctgttttgc agccacttga
     5341 ttttgttcaa tggtgtcatt ggatgaatcg gcgtcccagt ctgccgataa aattctttgc
     5401 caagcacggt atatttcagt ttttctgctt cgtccgctgc tcggttatct gcccaagtga
     5461 tcattcgaga taacggtgtt ttctcttcat ccaacaatat caggccatgc atcgcagagg
     5521 agaaggagac aaattgaatg attgtgtctg aaggcaactg tttaattact tcttttataa
     5581 cggtgcagac accttgaaag atcaattcct gatcttgttc tgccatccct tgtgtttctt
     5641 ggatcaaagg gtagcgaaaa taagcggatg ctgcttcttg ggcttcatct gtgaaagcaa
     5701 ctgctttggt ttgtgtggtt ccgatatcta atccgattga aacgttcatt ttttcacctc
     5761 aaattttatg tatgtacaaa aatttagttt aagcttgcgt atcttggttt tgaaaagggg
     5821 gagatggcgt gttactttat tttatcattg gttgctgtat cggatcattt ttatgtttaa
     5881 ccgcccagcg gattccgtta ggtcactcta ttatttatcc tagatctcat tgtgtcaaat
     5941 gctgtcgttc gctttcttgg tatgaattga ttccgattct ctcgatcatc gtccaacgat
     6001 ttcgctgccg ctattgcagg tgtcggttgc ccttctacta tttgttggca gaagccttat
     6061 gtggtggtct ttttgcctgg ttctttactt tttcttccgc aagaaatttt tcgacgttca
     6121 tatggatgct ttcggctttg ctattttcgt tgatggattt attttacttc atggtccatt
     6181 ctcacacgct attctgttct tgggcaattt tatggatttt ttggttgcag gccggcgtct
     6241 tccaatggca aagtgtgctg ttaacgttta tcgtaggcgg tgtgtgcctt cgctatggtc
     6301 aatcctatct cggagctggc gatactctgt tgatgctttc ttggagtggt ggcttgtctc
     6361 tagaagagtt gcttcagatc ttgtttcttg ctagtctgtt tggaatatcc gttttttctg
     6421 tttactttat ctgtcataaa aaaaagttgg aaaagctgcc tttcattcct tttttgagca
     6481 gtgcgttgct gattgtcctt catcttaggt attgaaaaaa ggttgtgaca aacgacttgt
     6541 cacaacctct gatatatagt gttctcttac caaatccttg tgacttggag ctaggcgaca
     6601 ggttcacaac ctctccacta aatggtgttc aatcagctga ccctcagtat tagctcaaaa
     6661 tgctggtttt acaacctctg atatacggtg ttccataagt agctttttcg gcttcttatt
     6721 tcttgacgcc ccaatgtcga cgaaggaaac ctgcacgtcc agaaccttct cgataaagat
     6781 tgtaatggag cttgtttttc ttgtaataat cctgatggta atcttctgca ggataaaatt
     6841 cgcttcttgg ttcgattttt gtcacgattg gcttatcgaa tcgcccgctg tcttgcaatg
     6901 cttgttttga acgctctgca atatttcgtt cttcttcatc attatagtag atcactggac
     6961 gataagaatc accgcgatca gcgaactgtc ctaatgcgtc agtcggatcg gtttgctgcc
     7021 aatagatttc caccaactga tcataagaaa taacttctgg atcatattcg atctctactg
     7081 cttctgtatg ccctgttgtg cctgttgtta cttgttcata agttggattt ggtacatgcc
     7141 cgccagtata accggatgtc acagaaatga tccctggctg ctcttcaaat ggtttgacca
     7201 tacaccaaaa gcatccgcct gcgaaaactg ctttttttgt catataaaat cccccttttt
     7261 tctcatcata ccttgatttc caaaaggagg ataacgtttt gcttgtgaca gaaaaaacgg
     7321 aaactattat atacgtgata cggcttctga taaatgccaa aacgaacgta cttgatcaaa
     7381 aacggttaaa tcaggaatca catattcagc agaaggaaga tttatgtttt cacctattcc
     7441 caatacatgc atgttaccag ccaaagctgc ttgacaacca gcttctgaat cttcaatgac
     7501 caagcatgca tttggcggta cattcaattg ctgtgctcct ttgagaaaaa cttctggatc
     7561 aggttttgct tttgatactt gcgtgccgtc aataaggaca tcaaaataac ttgtcaaatt
     7621 cgttttttct aaaatgaggc gtgcattttt gcttgctgat cccaatccga tcttgatgtt
     7681 tgtttttttc aagtactcaa gaacagctat tgcaccaggc aaaacggatg tttcatccat
     7741 tttttgaatc gcttgaagat atagattgtt tttttgctca gcaaaggctg atttctcttt
     7801 ttctgagaat ttctgttcca tttttccaaa tgacagcaat cgctctagtg actcggttct
     7861 ggatattcct ttcagttgct cgttttcttt ctcagtgaat ggaatggaca gctggttagc
     7921 taaaaccagc cacgcttcgt aatgataacg agctgtgtct acaagaacgc catctaaatc
     7981 gaatagtgct gctttgattt tcatcgatta cctccttgaa aatgaacgac ttgaagtaaa
     8041 gatgcgatgt ttttagctgt gcgatgaaga ttatcagtgg cttgctgtaa agcttcttgt
     8101 aaaggcacag ctgcaggaag gatcggaaaa acggccgaaa tgccaacatc tgctgctttt
     8161 tcgcttcctt taccaacact tccgcaaata gctatcacag gaacgccctc tggtactctt
     8221 ctagcaatcc caactggcgc ttttcctttt agactttgtc catctagacg tccttcgcct
     8281 acgattacga ggtctgcttg ctgtacccgt ttatcgaact ccagtaaatc caatacgtag
     8341 tcgatgccag agactatttt ccctgaagca aatgctaaca gtcctgcacc cattccacca
     8401 ccagcactac tgcccgatgc ctcgattact tgaggggcaa atctcatata aaaatcatgc
     8461 atggcttggt cagccactgc taattcattt tctttcaagc ctttttgtgg tccgaaaaca
     8521 aaagaagcgc cttcttttcc gcataaaaga ttagtaacat cagtaatgac agtgattttg
     8581 gctttaatca gctcaatagg cacgttcgtt ttatcaactg tagcaatttt acctagattt
     8641 tctcccagtg cttctacttt ttgattatct tgatcgtaaa aagcataacc tagaccttct
     8701 gccatgccga tgccgccatc gttggtacta cttccgccaa cgccgatgat gatgtgtcga
     8761 acaccttgtt gcaacaattg tcgtatcagt tctcctactc ctcgcgtgga taacctaagt
     8821 ggctgttgtt ggtttagtgg gacatctttc aatccgacga tttctgccat ttcaaaaatc
     8881 gcagtttgac cgtctgaagc gtactggatt tctttgtttt tgccaaatgc attagtaacg
     8941 actgctgttt gccgcttcaa ccttaaactg tctgctaatg atgaaaccgt tccttctcct
     9001 ccatcgccta taggtagcag atcatattca gcctgaacaa aaacttcttg aaaacctgat
     9061 tgaatggctt gtgctgcctc taaggctgtc agactttctt tgaaagaatc tggcgcaatc
     9121 agaatacgca ttattttccc ctccgttcca agatcagttc tttttccagc tggtattggt
     9181 ctccattgac aaaaataggt aatgtttctt tcgtctcttt ttttattact atttgttttt
     9241 ttgtcaaaac aaatagcaac ctgtgatttt tccaaataaa aggaaaagaa attttttccc
     9301 atgctgaagg aagtcttgga gaaatagaaa gagttccatt cgttactgta atacctgaaa
     9361 aaccaaatat aactgctagc catagtgctc ctaatgaagc agcatgaatt ccgtcatctg
     9421 atgaatgcgg atctggtcct aaatcaatca ggcacgcttc ttggaataaa tgatacgccc
     9481 attccatttc ttggatacgg caagcctcta tggcgtgaat cgctttactc aacgatgagt
     9541 cgtggattgt tttgttttca tagtaatgca aattcttaga aacgattggt tgagaaaaat
     9601 actcaggtaa taagtataac agcatgacaa gatctgcttg cttgctgact tggagttcat
     9661 ttacttcgct acgggcataa tcaagtaaaa tagactggct gccaggacgt tctttgtaag
     9721 tggtcaaatc aataactggc ttactgagat acgtgtcatc ttgcggaatc agctgttctt
     9781 tgttaggcac tggcaaatat aacttatcta aaaaattttg acattctttc aaaaaaagcg
     9841 gatcgccagc ttgatattta acagctaagg ccacattata atgcgccaga tagttcgtat
     9901 atgcattatt gtcgatatat tctgtgtatt catctggacc aatgactctg tgtatctcta
     9961 aatgatcatc gacttttaca gctctgctca gccaaaaagc tgctgtttca tgaatgagcg
    10021 tttttccctt tgtttctaaa aacgattgat cctctgttgc ttggacatac tcttgaacgg
    10081 cataagcaag gtctgcactg atatgatgtt ctgcggcagc tgaagcaact tcttgtcttg
    10141 ttccggtttt gatattcaat gcagcaaatt tgggtgtctc ttcctgacca gtgtgtgcgc
    10201 tttcccatgg gaaaagtgca ccttgatagt gataatctct tgctttttgc cgtgcgcctt
    10261 ctaatcgatc ataacggtac agcagcaaat tctttgctac ttcaggaaac tgatataaaa
    10321 agaaaggcag gataaaaatt tccgtatccc agaaaacatg ccctttataa ccttcgccag
    10381 ttagtccctt cgcccctaca ctaacacttg gatcattgcg gggagtcata atctgtaaat
    10441 ggtaagcagc gaagtccatc gctaactgat cgaaagaaga atcgccttgg atcgttatcc
    10501 gatgctgatg ccaaaaatct gtccaataag acatcgactg ctctaaaatt tctgtataac
    10561 taacgctttt ctgggcttgg gcaatacttt tcttaaacgg gtctgtttgc tgaagagagg
    10621 tagagagcac gatgatcttc tccagcgtaa aggacgtatt ttttctgatc ttttctgaaa
    10681 aggaagcggt gatccggcga ttttttgctt ggaatacacc tgctttattg aatttagctg
    10741 caatcccaat atcaatttta ctttctgttg tctggtagga tgcactgatc aaatcgtcac
    10801 caaatactcg cagttctttt tcaaccaact gctgtgtacc aaaattggtt tgctgtccat
    10861 cgatacccgt tttcacatct acttggcagt cttcggtcaa cggcttgatg accactttca
    10921 gggctgcctg atggagatta ttttgatcag caacacgaca agtaatgact tccacttttt
    10981 ttccttgagc agtttcaatg agcgcatagc gtttaagctc tcccatttct aaatctagtg
    11041 cccgctcgta tgcaagaact ttcatcgtct caagagaaaa atgttcaccg tcgattttta
    11101 tttcccatct gatgacatct gggagattca ctaactcggc tgattctgct cctaaaggtc
    11161 ggttatatac accagctgca aacattcccc ttacttgatt ccgatagtct tcctcctggc
    11221 ttcctctgat accaaaaaat ccatttccaa tggtcatctg actcgcatac ttattgatat
    11281 actctaaatc aaactgtttt tcttctactt tcataaggta ataacttctc ctgtttctgc
    11341 cgaacgatat aaattttcca ccagattctg gatgacatat ccttgccagc catctgcaat
    11401 catctgagtc tggtcaccga gcgcccgatt cacaaaatga gccatactta gttcatgtgg
    11461 gtcattaact ggcgtggtct tttgtaagga aaccagcttt ccatctacat cttcgaatag
    11521 ttcgcctgga tacagattag cacctgcctg atcgccacaa aaagccacat tcagtttgtc
    11581 ttctctgata ttcaaagcaa acgaagtatt cagttggatg atcttgccgt tttctaattc
    11641 cagtgtgcca aaaattgcat cttctactgt gaatttgctt ggatcccatt tcccgaattt
    11701 ccctgaattt tttttcgtac caattttttg gaagctgtac gcccgaacgc tttttacttt
    11761 aggaaatcca gtaatataca tcgctgtatc aagcatgtga atgccataat cgatcaaagg
    11821 cccgcctcct tgaagttctt tgtcgataaa cgttccccag cctggaacac cagaccgacg
    11881 aagcgcattc gcttctacgt aataaatatc cccgagaaca tcaagatgat tcttgatcat
    11941 tgttgcttcg tcagaaaaac gatgctggaa tccataagcc aaaatctgcc cgtttttatc
    12001 ggctgtctct gccatctttc ttgcttgatc agctgtcata gctggtggtt tctcgcacat
    12061 cactgaagca ttagcttcga gggcagccaa tacatgttcg aagtgaaagc gattgggtgt
    12121 acagacactc acgatatcgg gagaaatctc attcagcatt tgtgtaactg atgtatacgc
    12181 atgagaaaat cctgtacgtc ggcaaaagtc ttctgctcgc tggctgactg gatcaaccac
    12241 agcggccaat tctaagacat ctgatctttt ttgataataa gcagcatgta ctttttctgc
    12301 tacttgtcca gcgcctatga ttgctacttt cttcatttat ttctccctcc tttgcttatt
    12361 tacttattgt tattctttct ctattctttc tctaaatcat ttaaatgacg cgctgtttcc
    12421 agacagtttt tcaagtattg caatgattcc tgataaattt tcacttcatt ttctcctcgc
    12481 aaacgacatt catataccat ataaccttca tactcatttt gaagtagcgt ttgaaaatgg
    12541 cgagtaaaat caattgcgcc agatcctggt tggtagcgat gattatctgc taaatggata
    12601 tgacccacat actctttttg cgtagtcaat gcttttgaaa tatcatcttc ttcaatattc
    12661 atatgataaa aatcagcgat gatctgcaca tgtttcaatt gattagagtc aatgtaatga
    12721 cgcgcatccg ctaacaaatt gatcatatgg tcttggtatc ggttcaaagg ctccaaataa
    12781 ataaccgtac ctgtttcttc ggctacttta tctaaataac gaagtgattc gctgactgct
    12841 ttatagtctc cttctttgct tcggggtgaa accatcggcg gtaatcgata agtgaacatt
    12901 ccccaagctg ctgggataac gattcctttg cctccaacct cgcttagtgc acgcaaaatc
    12961 tcagcaattt gacgtacacc attaagacgc cgttcttcga taaagtcacc gatccaccca
    13021 tcgtagccgt tacaagctgt cactactggg atccctgttt ctttgattgc ctgtttgact
    13081 gtgtcgatat tttctattaa caacttaccg tcgatttcgt attcttcaaa tccacagtct
    13141 ttgataaatc taaatttttc taaaatgtct cttgggaaaa atgcttgatc ttgcgtaccg
    13201 aatttcattg ttctcctcct cggacttgaa cgcccatttt gatactttct tctgggtgct
    13261 ggtctacata acgcatgtaa gcttctgcgc tttctgcaaa tggcacaact ggatcaatga
    13321 tttcttcaca atccaagtaa ccgttcatca ataattccca acatgtgtcc tcgattcgtt
    13381 tacggttcca tcttgggtaa tctgggtttg gttcactaga tgctcgagca aagacgattt
    13441 ttgcattatt gaaatgtgct tcgcgaccaa gattaaaccc agctggaaaa ggtttagcaa
    13501 aggcaacata agaaatgatc ccgccatagc cgatccctct taaagaagct tgtaatgcgg
    13561 cagcactgcc actcgtttca ataactgaat ctactccacg tttatctgtc aattccttta
    13621 gacgaaaacc gacatctgct ttcataggat ctagcgtgta atcggcaccg tatttttctg
    13681 ctaattctct acgctgcgct atgggatcga tcccaacgat ggtagtagca cctgcttttt
    13741 tagctaattg aatcgcaatt tgcccgatag cgcctaaacc gaagattgct acagcatcac
    13801 ctgctcgaac atgtgcttcc cggataccac ctaaagcgaa ttgagcagga tcgtaacata
    13861 ctgcattttt ccattgatcc aaccgatcaa gtttacgtag tttataatta tcaacaccgt
    13921 ttgtaatcag cgtttcacga atgggtccat aagtgcagac taactctcct ttttgatact
    13981 ctgtcaccaa actgcccgtt tcttcgatcg taccgacaat catatttcct aattgaaatt
    14041 cgccaaatac cacaccgcgt tggcttcctt cttctcttgg caagaatagg cgccattctt
    14101 ctgaaaattc ttcatcgata aatggactta atcctctaaa gtcgacaatt tctgttcctg
    14161 gttttggagt agcgaaaatt actcggatac gtacttcgtt cttttcgatc tcacggtctt
    14221 cgtaatcttt gaatccggct attcttggtt ctaatgcaac taattttttc atcttttctt
    14281 tcctctttct tttcttttta tccttttacg ccgccttcga ctaaatcacc tttaataaat
    14341 cgttcagata acgcatacat aatgatgact ggtaaagcgg tcaccaatga agcggccatc
    14401 attcgtcccc aaatgtagtc tggtgtactg aataatgtgt tcaaaccgat tggtaaagta
    14461 aatttttcgg aggtcgacag gaaaatcgaa gcaaataaat aatcattcca cgaaaccata
    14521 aagcaataaa cgaagacaga aatgattcca gaaatagcca atggtataat gatccgaata
    14581 atgatctgga tccggctaag accatcgatc attgctgctt cttcaatatc agtcggaatc
    14641 gtgtcaaaat aactttttag catgaatatc gcagtaggaa gtgtttgaac gaccatcgtg
    14701 ataatcagtg ctgtattggt atcatacaaa cctagaccgg aaatgatttt aaataatgga
    14761 acaacgatca aaataccgga aaacatataa acggtgtaaa agctagcatt gattttcgag
    14821 cgagctttga agcgtagttt tgacaaagcg tatgccccgc tgatcccaat cactactgca
    14881 gttacggagg ctatcaagga gatcactaaa ctatttttga aatacatgat gaatggaaaa
    14941 atctcagcat tgaaaatatc ctgataatgt tcaaaagtcc attctttcgg aaaaatagtt
    15001 tgtgtcgttg caattgcttc ttgtgaatcc ttgaatgatg tcatcagcat gatcaaaaat
    15061 ggaaacaaat tgacagctaa tagcaacaaa atcgtcgcgt aaaataagat tttcctgctc
    15121 tttttatttt tcatttcaag tccaccttct ttcgtgagag caagacgacc gctgcaataa
    15181 tgatgaacaa tagaatcgat atagctgctg cttttccaag atcattgaac gcaaaggctg
    15241 tctgatacaa ataaacgcct aagatattga ctttattcgt cagtagatag acatctgtaa
    15301 acatatagaa catccagata gatcgtaaaa caatcacagt tgctagtact ggtttgattg
    15361 ccggcagtgt cacgatcttg aacttctgcc aagcatttgc accgtccatt tcagctgctt
    15421 cataaagcgt tttgtcgact gtttgcaaaa tagccaaaaa tgaaataaac gcatacggat
    15481 agtatctcca gatagcaaac aaaacgacca taaagaagct agcaactggc tggtcgaacc
    15541 acagcggtgc ttcgttgaaa agctgcaacc tatctactaa cagatcattg acgattccat
    15601 aactattatt gaacatatat ttccaagcaa aaatcaaaga aatagatggc gtaacataac
    15661 ttaataaaat cagtgaacgc accgttttcc taaatctgaa cttgcggtta agcaaaatag
    15721 ctacggctaa tcctaaagct gtactgcctg ctacaactaa gaatgtgtaa gcaactgtta
    15781 aacctaaaga acgataaaat tcactatcac ttaaaatttc aagataattg cttaatccga
    15841 taaaaacggc gtctgagtta ggattcagcg gcatatcgaa aaaactgatc tgaatattgg
    15901 aaagcattgg aatcagcact aaaacagcta ataaaagcat gcttggtgct aaaagtccca
    15961 tggctaattt taagtcagaa tgtttccttg gacgattcat tgaaacctcc ccctgataat
    16021 ctgccaatag ctatctactt ttttcgaaca ccattggcag aacttcttgt tatttcgtat
    16081 ttttttgtgc gtcagctaaa gcttgatcga catcttcgtt tccgacagtt acttggttca
    16141 ctgcttttcc gatcacacct gagcttgtga tatctcccat tttcgtgaaa ttcttttccc
    16201 cgactagtcc aaagacttgt acttcatcaa aagcactagc aatctcagat ggcagctcgc
    16261 caaaagactt gatgacttca ttttctttgt aaccatccag ttcgactact tgtttattca
    16321 ctggttgagc accaccagga gacatcaaga cccacttttc catgtttttc ggttgagata
    16381 agaaagctgt gaatgcttca gcagcctctt tttgtttctc gtctagacca gaagaaatcg
    16441 tcaatccaga tactgtgcca tagacagctt gatctttttg tgttgggata gcaaaaccga
    16501 tattttctga gttccccgct tcataaacag aaggcaaaat gtaagttgaa tagattgcca
    16561 ttggtgctgt tccattcatg aaagcatctt tgatttctgt cacatcatta gagcctggca
    16621 tcgtgtattg ggataagttt tgataaaacg acaatgcttc tctcatcttt tccgtatcga
    16681 tcgttacctc gccttcatca tccaaaacat ttgctccatt tgacaaagca aattgagaga
    16741 acgcttgctc actcattgtt ccttcagcag taggcatagc aattccatat tgtttattct
    16801 ctttatctgt gaaatgttga gcaattttta aaatatcatc ccaattttct ggttctgaaa
    16861 aaccagcttc tgataatttt tctttgtcat accaaatacc ttgaacccag ccgctaattg
    16921 gtgcagcaat atagcttttt ccatcttctg aacgaacaag gtttttggct ccgtcataat
    16981 aattatcttc tccagcttcc tcgataacgt tttggactgc cgtctgatca atcagttcat
    17041 ctttatccat cactttagca aaatcctgac tgacttccat tacttctggt aatttaccgg
    17101 aacgagctaa cgtaacgatt tttgtattga acgctgattc ttctaccggc acttgcttga
    17161 ttttgatgtc tggattttct ttttcaaaat cagcaaccag ttcattgatc acacttaatc
    17221 gttcctgttc gacagaagag tgcatgaatt gaatcgttgt ctgttgtgat tcgttagagc
    17281 tttgactttc accacaagca gtaagaagaa atccagtagc tagcagacag gtagtaacaa
    17341 taattttttt catttttttc cacctcttat tttattattt tcagccacat atattcatac
    17401 ggctggaata tcaactttcg ttcttgactg caatggataa tttgtccaga aaacaggtct
    17461 aggtaatctc cttttggtaa aagataacct gctgattgat tggaaaaatt atggataact
    17521 aagactttct cttccttcgc ttcgcgaata aaactgataa aagaatctgc tgagtccagt
    17581 aagtaaatca aactattcgg atggaaggct ttttcttttt ttcgaataga tagcatctgg
    17641 gtcaattcac catatactgc tgttcttaat ggatctgtct gcaactcttt ctcaatatct
    17701 gctaacaaaa atttttgccg attgattttt cgattctctc ctgttttttc tacgcctttg
    17761 taatcatttc gacttcccag cacactttga atataaattg ccggcacgcc tggtaacgtt
    17821 accaaaaatg aatgagcagc taaaaagcgt ttgcttctcc attccgtcgt atctgttgga
    17881 gaacttaacg catctagata cgtagcgttt atctcatatg gactggtcgt tccatctggg
    17941 tttttcttgt agtttactcg ggcaccctct ttcttcagct gttccactaa ttcaaggatc
    18001 gttttttccg gcagaatacc tcgtaccgga ttcaatccga taccgtcatg ggaggctaaa
    18061 aaattaaaaa aagttgtttt tttcgatgcg aaatcgatgg tttttaacca ttctttcaac
    18121 ttacttgtat tacccgaacg aatcgcttga agggtcaaag gtggtagcga aaactgatag
    18181 accatctgtg cttcattctc gccattccca aaataactga tattatcttt gtgtggcaca
    18241 tttgtctcag tgatcaatac cgttcctgga gctgcttcat cagcgattgc acggaataat
    18301 tggatgattt tatgcgtttc tggcaggtgt atacaagaag ttcctagttc tttccataaa
    18361 aatcccacag cgtccaagcg aataaatgaa gcgccttttt ctaagtaaaa caaaagaata
    18421 tcgatcattt tcaataaaac ttctggattt ttaaaattca agtctacttg atcttcgcta
    18481 aaagtcgtcc atacgtgttt ttctttcccg tttttgtcaa tgaacgttgt aagtaacggg
    18541 ctggtccttg gtctagtcac gctgcttgta tctgtatcca gtggcaaatc aataaaaaaa
    18601 tctttgtacg tatcattcaa gtttaaatat tcctgaaacc aagtactttc ggcagacata
    18661 tgattgcaga caaagtcgaa cattaaccgt gtttcgcttc tcatttcttc tatatcttcc
    18721 catgtgccca aacggtcgtt gattgcctga taatttacaa cggagaaacc atcatcagat
    18781 gtatatggga aaaatggcaa gaaatgtaca actctaaatg tatctgccaa atacttttga
    18841 taaaatttct taaatgtacg caaagttggt tgctgctctt caaaaaattg atccccataa
    18901 gtaatcaaga aaatatcttc ttcgtcccaa tgcagtttgc ctgcctggcc tttcttctgc
    18961 tgataaacac tgactttttg ttcaatcata gaggcggttt tctcagacaa atctgagtcg
    19021 cgataaatcg cttgtaaaag ttttgtatac atcggattcc cctttctttt ttgggagcgt
    19081 tcccaacgat ttgatcatat tatggaaaac gcttactgtc aatagcattt ttttaaataa
    19141 ttttgatata cacaaaaata caaataagaa agcttatgta tcaataatta atagcttctt
    19201 aaaaaaatat ggtctctcat gatcatgcaa gctattccta aattttcaaa atcatagaca
    19261 aaaaagaatg tctattgtac tatggaatta ttctaaaacg ctcccaaaaa ggaggtgcat
    19321 ataaaattga aacctactat ttatgacatt gcgaaaaaag ctaatgtctc aaaatcaact
    19381 gtctccagag tattaaataa ccagccaaat atctctgata aagcaaaagc aagtgtactt
    19441 caagcaatca aagaattgaa ttatgaaccg agcaaaattg caagaggact gtcaggaaat
    19501 ggttttgacg cgattctagc attatctcat cgtacttctc atacgaccta tggcaatcct
    19561 tttttttcag atgttattca atccatttct gccgtttctg aacaaaataa cttcgacttg
    19621 attctacaaa cggcaaaaac accagaagaa gagctagaaa aatgcgcgaa aaagatccaa
    19681 gaaaaaatga tccgaggaat tattatttta agctctacga tggatgaaac acttcttttt
    19741 gaattggatc aatacaatat tcctattgtg gtaatcggaa aagtcggaaa cgactacacg
    19801 aacatctact cagtggacac ggataatttc caggatagtt acgatttggt ggaaacactg
    19861 atcaagaatg gacaccgtca tatcggctgt ctccatgctc cgacagaagt ttatgtatcc
    19921 aatgatcgtc tcaatggtta ccgtcaatgc ttatttgatc accacctaga tatccgtaat
    19981 gaatggatta tagacggcgg ctttcgctta gaagatagca tggcagcaat gaatgattta
    20041 atgaagctac gagaaagacc aactgcgatt ttttctaccg atgcattaaa atcattgagt
    20101 atttacaaag tggcagaaca atctggaaag cagatacctg atgatctttc gatcgcagga
    20161 ttcagtgatc ccttattgtc ctccctattt tcgccacagt tgactcggat agacattccg
    20221 acaaatcagt taggaatcaa agccacagaa cttttattcc aactaatcca ccaccaatac
    20281 cctgaagaaa aaagaatcat tctttcaacc gagattactc tcacagattc gatccaacaa
    20341 caagaaatgt agaacagaat tataaaagaa cagaaacact gaggataagt cagttactct
    20401 gttctttttt caatcactca tccctcgtta ccccctcccg gtattttagc gaatttttct
    20461 tattttccat ctgttttaga tagctgtgga taactataat gtttgtgtta aactattttt
    20521 tccacaggga agaaaaaact atttttaacc acgttgtgta taagttttta tagttcgttt
    20581 ttttaaacac atttaccctt atttcaatcc atgttatttg gggatactta tctctaaaac
    20641 ataaatagaa taacagacat gtctactggt tttgttttat tcgaggaatt attcaataat
    20701 agctagcaaa atattaaaaa taaccgctct atcttcccat caatttattt tgcattcaaa
    20761 aaatgatcta tctttacttt tgttgattga cttctcttgg aagatgatcg ttccgatacc
    20821 ttaattggat acgaaccgat gttgttaaga tcctttccca cttttcttct agttagacaa
    20881 attattttag acaaaccaaa aaagaggttg tgacaataat ttgtcacaac ctctttttta
    20941 aaatatctat tttgtttgat tgttcattgc gtcttctgca gccatttgcg cttcaatatc
    21001 tgaaatactg tagacatttt cgcttgctac accgacttct tttggttcca tgtcacgata
    21061 acgttgcata ccagtaccag ctgggatgat cttacctata ataacatttt ctttcaaacc
    21121 aagaagagga tctttcttac cgcggatcgc agcatccgtc aagacacgag ttgtttcttg
    21181 gaatgatgca gcagataaga aactatttgt ttctaatgat gcttttgtga tacctagtaa
    21241 gacaggtcga gaagttgcag gaacgcctcc tgagactagc gtattgtagt tttgatcttt
    21301 aaagtctgcg atgtctaata atgtacctgg taagatatct gtatcacctg gatccattac
    21361 acgaacttta cgcagcattt gacgaaccat tacttcgata tgcttgtcgc cgatttctac
    21421 cccttgcata cggtaaacac gttgtacttc acgaagcaag tagttttcaa cagataatac
    21481 atcgcgaact tgcaacaatt gttttggatc gattgatcct tctgtcaatg gtgcaccacg
    21541 gtggatcata tcgccttctg ctactttcat acgtgctgta taaggaacag tatacgtacg
    21601 tgtatctgtt tttcctttga ttgttacttc tttggtacgt gttgcaggat cttctgagat
    21661 atcgatgaca tcccctgtta cttctgtgat cactgcttgc cctttcggat tacgtgcttc
    21721 aaagatttct tggacacgag gaagaccttg agtgatatcg tctccggcaa ccccacctgt
    21781 atggaatgta cgcatcgtca actgagtacc aggttcaccg attgattgag cggcgatcgt
    21841 accaactgct tctccgactt caacgtcaga gccagttgca aggttgcgtc cgtaacaatg
    21901 tttgcagaca ccatgttttg tattacatgt gaagacagaa cggattgtca ctgtttcgac
    21961 acctgcatca acgatttgtt ttgcgatgtc ttcggaaatc aattggtttc ctgcaatgat
    22021 caattcattt gtttcaggat gacgtacttc tttacgggtg taacgtccca ctaaacgatc
    22081 ttctaatgat tcgatgattt catttccctc gcggatggct tggatttcaa gacctcggtc
    22141 agtaccgcaa tcttcttcac ggatgattac atcttgggcc acgtcaacca gacgacgagt
    22201 caagtaacct gaatcggcag tcttcaaggc tgtatcggtc atccctttac gagccccgtg
    22261 agttgagata aacatttcca agacggatag tccttcacgg aagttcgaaa tgattggcaa
    22321 ttccatgatt cgaccattag gagcggccat caaaccacgc attccggcaa gctgtgtaaa
    22381 gttggaaatg ttaccacgag ctccagaatc agacatcatg aagattggat ttttcgcttc
    22441 caaaccttcc atcagttttt gttggatgct gtcttttgca gcattccaaa cagcaatgac
    22501 acgttcataa cgttcgtcat ctgtgatcag accacgacgg aattgttttg taatagtttc
    22561 aacttgttta tgggcatttt caatgatttc ttgtttttca ttcagtacac tgatgtcagc
    22621 gatccctact gtgataccgg catgtgtcga atgtttgtac cctaagtctt tcatacggtc
    22681 aagcatctta gaagtttctt ttactttgaa tcgtttgaag acttctgcga tgatgtttcc
    22741 taagtttttc ttcttgaacg gtaatactaa ttcttgctct ttgatatgtg caggaatatc
    22801 agtaccagct tcaacaaagt atttgtccgg tgtttggacg gtcaagttga agtctgttgg
    22861 ttcattcaga taagggaatt caggaggcat gatctcgttg aagatgatct taccaactgt
    22921 tgtgatcatc gtacgttctt tttgccattc agtaaatggt ttttcaccta atgagttagg
    22981 gttcacaccg atacgtgaat gtaaatgaac atatccgttg cgccatgcga taacggcttc
    23041 gttttgatct ctgaagacca ttccttcgcc ttcgcgtcct tcttcttcca ttgtcaagta
    23101 gtagttacct agaaccatat cttgagaagg agtaacaact ggtttaccat ctttagggtt
    23161 caagatgttt tgagcagcta acatcaacat acgtgcttct gcttgtgctt cttcattcaa
    23221 tggaacgtgg acagccattt ggtctccatc gaaatcggca ttataagctt cacatacaag
    23281 tggatgcaaa cggattgcgc gtccttgtac taaaactggt tcgaatgctt ggataccaag
    23341 tcggtgaagc gtaggtgccc ggttcagcaa tactgggtgt tccttgatta cgtcttctaa
    23401 aacatcccat acttcgtctt cttgacgttc gattttacgt ttcgcatttt tgatgtttga
    23461 agctagttcg cgttgaacaa gctcacgcat cacgaatggt ttgaataatt caatggccat
    23521 ttcttttgga agtccacatt ggtacatctt caagaaaggt ccaacaacga taacggaacg
    23581 acctgagtag tcgacacgtt tacctagcaa gttttgacgg aaacgacctt gtttcccttt
    23641 caacatatga gaaagagatt tcaatgggcg gttacctggt ccagtaactg gacgtccgcg
    23701 acgaccgtta tcgatcaatg catctactgc ttcttgcagc atacgttttt cattttgaac
    23761 gatgattcca ggtgcattca agtctaacaa acgtttcaaa cggttattac ggttgattac
    23821 acgacggtaa agatcattca agtcacttgt tgcaaaacgt ccaccttcta attgaaccat
    23881 tgggcgtaaa tctggtggaa taacagggat aacgtccata accatccagc ttggttgatt
    23941 tcctgaagcg cggaatgctt ctaaaatatc caaacggcgg attgcacgtg tacgtttttg
    24001 tccttgtgca gatttcaatt cttctttcaa ttcggctgct tctgcatcta gatcaacatt
    24061 atcaagtaat tgtttaatcg cttctgcacc catagcagct tggaattctt gtccatattg
    24121 ttcacgtttt tcgcgatatt cgcgttctgt caacaattgt tttttctcta aattcgtatt
    24181 tcctggttcg atgactacat atgaagcaaa ataaatgatt tcttccaaag cgcgtgggct
    24241 catgtctaat acaagaccca tacgtgaagg aatcccttta aagtaccaga tatgtgaaac
    24301 aggagctgct aattcaatat gtcccatgcg ttcacggcga actttcgaac gagtaacttc
    24361 cactccgcat cgatcacaaa cgattccttt ataacggata cgtttgtatt ttccgcaggc
    24421 acattcccag tcttttgtag gaccaaaaat ccgctcacag aacaaccctt cgcgttcagg
    24481 ttttaaggta cgatagttaa tcgtctcagg tttttttact tcaccgtaag accagcttct
    24541 gattttttcg ggagaagcca aacctatttg catactttcg aatttattta catcgatcaa
    24601 aaggggttcc ctccatttca tttaatggca aaccgcaaga cggctgatct tcgaccgcct
    24661 tgcagctcat agaccggatt agtctaaatt atcttctgcg gtttcaaagt tttggatgac
    24721 atcgtctttt tcatcttcca cttgttcagc agctttcttt tctaattctt tggcagtttg
    24781 ctgttcggca aattttgtca aagcatcaac agtgatcaaa tcgtcgtctt catcgtccat
    24841 gtcgcgaagt tcgatctctg tttcttcgat atccaagaca cgcatgtcca gacctagtga
    24901 ttgcaattct ttcaccaata cgcggaatga ttccggtaca cctggttttg gaattggttc
    24961 acccttgacg attgcttcgt atgttttcac acgtccgact acgtcatctg atttgtatgt
    25021 caagatttct tgtaaagtat aagccgcacc gtaagcttcc agtgcccata cttccatttc
    25081 accaaaacgt tgtccaccaa attgagcttt acctcccaat ggttgttgtg taaccaatga
    25141 gtaaggtccg attgaacgtg catgcaattt gtcatcgacc atgtgagcaa gtttgatcat
    25201 gtacatcaca ccgacagaca cacgtccatc aaatggttca cctgtacgtc cgtcgtacag
    25261 aacagttttc gcatcgctgg ccataccggc ttcacgaact gtttcccata cgtcttcatc
    25321 acttgcacca tcaaatactg gcgttgctac gtggatacct aactgacgtg cagccatacc
    25381 caagtgtaat tccaatactt gcccgatgtt catacgtgaa ggcaccccta aaggattcag
    25441 catgatatcg attggtgttc catcaggtag gaatggcata tcttcttccg gcataatacg
    25501 ggatacaacc cctttatttc cgtgacgacc ggccatttta tccccttcat ggattttacg
    25561 tttttgaacg atataaacac gaactaacat gtttacgcct ggtgacaatt catctccagc
    25621 ttcacgagta aagattttca catcgtgaac gataccgccg ccgccatgtg gtacacgtaa
    25681 tgatgtatcg cggacttcac gagctttttc gccaaagatc gcatgcaata gacgttcttc
    25741 tgcagataat tctgttaccc cttttggcgt gactttccct acaagcaaat cgccatcttg
    25801 gacttctgca ccaatacgga tgatacccat ttcgtctaaa tcttttaatg cgtcttcccc
    25861 gacattaggg atttcgcgtg tgatttcttc aggtcctaat tttgtatcgc gggcttctga
    25921 ttcatattct tcaatatgga tagaagtgta tacgtcatct tttaccaaac gacggctcat
    25981 gatgatcgca tcttcatagt tgtacccttc ccaagtcatg aaaccaacta aaacgttttg
    26041 gcctagagcc atttcgcctt gttccataga tggtccgtct gccagagtat cccctttttc
    26101 gactttctca cccaaatgaa cgattggacg ttggttgtag cttgttccgg agtttgaacg
    26161 acggaatttt gttacatcgt atttatctaa tgcaccgttg tcgcggcgta cgcggatttc
    26221 agaagcatca acgtattcaa cgacaccatc atgtttacat aacaaagcag ctcctgagtc
    26281 atgcgctgat ttgtattcca taccagtacc aacccatggt gcttgaggat tgatcaacgg
    26341 cactgcttga cgctgcatgt tggcacccat caaggcacgg ttggagtcat cgttttccaa
    26401 gaaaggaata catgctgtcg caacggcaac tacttgttta ggtgatacgt ccatgtagtc
    26461 tactttatca atagatactt ctaagttttc actttgagca cgagccataa caacgtcttg
    26521 agcaaatgtt ccatcttcat tcaatgggct gtttgcttgg gctacgatgt aatggtcttc
    26581 gatatcagct gtcaagtagt cgatttggtc agtaacacga cctgtttcac ggtctacacg
    26641 acgataaggt gtttcgataa atccgaattt atttactttc gcataactgg acaaactatt
    26701 gatcaacccg atgtttgggc cttcaggagt ttcgatcgga cacatacggc catagtggga
    26761 gtagtgaacg tcacgaactt cataaccggc gcggtcacga gtcaatccgc caggtcctaa
    26821 ggcgaaaaga cgacgtttgt gtgtcaattc gccaagaggg tttgtttgat ccatgaactg
    26881 tgacaactga gaagaaccaa agaattcttt gatacttgcc acaactggac ggatattgat
    26941 caattgttga ggtgtcaatg tttcagtatc ttggatcgac attctttcac gaaccacacg
    27001 ttccatacgt gctagcccga tacggaattg gttttgtaaa agttcaccga ctgaacggat
    27061 acgacgattt cctaagtggt cgatatcatc gacattgccg ataccttcca tcaagttcaa
    27121 gaaatagctc attgaagcaa tgatgtcggc tggacgaacc gtttttacag ctgcttctgg
    27181 atagccgttt ccgatcacgt ttacttcacg ttcagggtct cttggtgaaa atactttgat
    27241 gacttggaca gtcattggat ctgtgaccac accgtcttct gatggatagt aagtcactga
    27301 atttaggcca ttatcaatga aaggagctag tgtttccatg acttggtgag tcagaactgt
    27361 tcctttttca acgatgatct cgcctgtttc tggatcaact aatgtttcag ctaatgtcag
    27421 attcaataga cgtgttttca aatctaattt tttgtttact ttgtaacgac caacgtttgc
    27481 taagtcgtaa cgttttggat cgaagaaacg agcattcagc aagttacgtg agctgtctgc
    27541 tgtttttggt tcgcctggac gcaaacgttc gtaaacgtct ttcaaacctt cttctgttct
    27601 tgagtcgctt gcgtttttat gcaagtcttt ttcgactgta ttgcgtaatg tttcgctgtc
    27661 gccgaagatt tcaaagatcg tatcatcgga accaaatcct aacgcacgaa ctaatacggt
    27721 taaaggaatc ttacgtgtac ggtcgatacg tacataagag atgtcttttg catcagtttc
    27781 catttctagc caagctccac ggttagggat aacagtagaa ccgaatcctt cttttccgtt
    27841 tttgtcgact tttccatgga aatagacacc tggtgaacgg actaactgag aaacgattac
    27901 tcgctctgcc ccgttgatga tgaatgttcc ttgttctgtc attaatggga aatcaccgaa
    27961 gaatacttct tgtgctttga tttcgccagt ctcgcggttt gtcaaacgca atgttacatg
    28021 taatggtgca gagtagtttg catcatgcgc acgagcttct gctactgtat actttggttc
    28081 tttcaattca taatctacaa attccaaaga tagattgcca ttgaagtcat cgattggcaa
    28141 aatgtcttca aacatttctc taagtccctc atctaagaac cattggtaag agtctgtttg
    28201 aatttcgatc aaatttggta attccaacac ttcactgatt cttgcgaaac ttctacgttc
    28261 gcgatgtttt ccgtatttta ctacgtgtcc agccaagctc ttcacccctc taaaatgtca
    28321 ttcaaaaaga ctacccgatc aattgttaca ataatatttc aaatattatt tttaggtaac
    28381 aacagacatt ttttgcggct tttaggcaaa aaaaaaccaa aaatagaaca ttctcttgaa
    28441 accttacttc gtgagcttct acttttgttt ccttatatta aagccgaaac ggacaatatt
    28501 tcgtttctgc cttttcttca tcagatagtt tttgcaactt catatactac acttattttc
    28561 tataaaagtc aacacttcct ggcgtttctt ctgctttttc gcaacatttt cttaatgttt
    28621 tcaattttat gttttcaatt atctgacaat tcttaattga cagacctttt tatctcggtt
    28681 atactttaga aaattcattt tgtggaggaa cttctatgcg aaccaacatc aaccaaatcg
    28741 caccaacaca actgaataac ttttacttta gctactttag ataggtttgt attcatcatg
    28801 atggatacaa acgttaaacg acagtttgta tctatgatgg ctaaagaact ttgtattgca
    28861 gcaaaaagcc acgtagacaa aatgctaagt ggctgagcag tgaaaataca aagtaaaagt
    28921 atcaagcatt ctactttttc aatttatttt ccgacttttc aatccatttg gacattttgt
    28981 ctgaatggat tttttgtttc ccaaaattaa ggaggaaaaa gaaatgaaaa aaaatataat
    29041 catcggatta ggcgctgccc tgctactagc tttaggaggt tgcgcacaaa aagacaattc
    29101 cgcagaggca tcagatggac aaaaaacaat tggcgtattg caacttgtac aacataactc
    29161 gttagatgct gcctataaag gattcaaaga aggattagca gaagaagggt ataaagaagg
    29221 cgataatcta acaatcgatt atcaaaatgc ccaaaacaat caagacaacc tgaaaagtat
    29281 gagtgagaaa ctcgtcaaag aagatcctga tttgctactt ggaatcgcca ctccagcagc
    29341 acaatcttta ttaaatgaaa caaccactat cccgatgact gtcacagcgg tcacagactt
    29401 ggaacaagcc catttagtca catccaatga aaaacctggc ggcaatgtga ctggcacgac
    29461 agatatggtg ccaatcgaaa aacaaatcgg cttactgtta aatatcgtgc cagacgccaa
    29521 aacaatcggc atcatgtata acaacggtga agcaaactct aagatccaag ctgatttggc
    29581 agaaaaagaa ttgaaaaaag cgggcgtcaa agtcaaagta ctgacagcaa attccacaaa
    29641 tgatgtccaa caagtgacga aaagtctagc taaagatgta gatggaatct atattcctac
    29701 tgataacacg ttcgcaagtg cggcaagtgt tgtcggtgaa gtcgcaaaag aaacaaaaac
    29761 acctgtcgtt gctggcgcag ttgatcaagt caaagacggc ggtctagcaa cagtaggaat
    29821 cgactacgaa agcttgggca aacaaacagg gatcatggca gcaaaaatct tagacggcaa
    29881 gaatccaagt gaacttcctg tcgaaacagc gaatgatttg gaactgtatg tcaataaaga
    29941 catggccaaa gcattaggca tcaatccaga cagcatcaaa gaagctgaat aaaacacagg
    30001 aggattctca tgacaaattt gctctctctt ttcttatcat ctgcatcgca aggggtctta
    30061 tgggccctac ttgcgattgg tgtatacgtc actttccgta tcttggacat tgccgacttg
    30121 actgctgaag gaagctttcc tttaggtgca gggatcgcgg cagtaagtat tacaaatggc
    30181 tatcatccat tgacagcttg tcttctaggc tttttaggga gtgcggcagc agggcttgtt
    30241 tccggactgc ttcacacaaa attgaaaatt cccgcccttc ttgcagggat catcaccatg
    30301 actggtttat attctgtcac atcccgtgtg atgggtgcgc cgaacatctc tttattgggt
    30361 cagccaacga tctttcattg ggtcgagtca ttcggcataa caaaagacaa cgctgtactg
    30421 atcgtcggtc tgctttttgc tttgatcgtt gtatgtttac ttgttttgtt cttacgtaca
    30481 gaaacaggac ttgctattcg tgcaactggc gataatctgg caatgagcga agcaaacggg
    30541 atcaacacag ataatatgaa gatcatcggt tatatgattt ctaatggatg cattgctctt
    30601 tctggctcct tgcttgcgca aaacaacggc tttgctgatt taaattctgg tattggaacg
    30661 atcgtaattg gacttgcttc tattatcatc gcagaagttt tgttacgtaa ccagccacta
    30721 tttctccgtc tcttgactgt cgttttaggt gcggtcattt atcgttttat tttagcgtta
    30781 gtcttcgaac tgaatgttga accaagtgat tcgaaacttg cttctgcttt cgtgctagtg
    30841 atctgtcttt ctttcccgca gatccagcaa aaattccatt tacctcaaaa aaagaaaggc
    30901 ggtagtgtca aatgaatgac gtcttaacat tggaaaatct ccatcatacc tttgaaaaag
    30961 gaacgatcaa cgaaaatcat gtcctgaaag gcattaatct ttccttagat cccggcgact
    31021 tcgtgacgat catcggtgga aatggggctg gtaaatctac tttgctgaac agcatcgccg
    31081 gtacattttc tgtcgatcag ggtcaaattt tattgaatgg aaaaaatatc acgaaaaaat
    31141 ctgttgtcga acgttcgaaa tccatcagtc gcgtattcca agatcctaaa ctagggacag
    31201 ccgttcgttt aacggtagaa gaaaatcttg ctcttgctat gaaacgagga aaaaaacgcg
    31261 gcttcttccg tggtgtcaaa ccacaagacc gaacattttt caaagaccag ctggttcggc
    31321 taaatttggg gttggaaaat cgtttatcct cagaaatcgg tttgctgtct ggaggacaac
    31381 gtcaagctat tacgttattg atggccacat tacagcgtcc agaactgatc ttattagatg
    31441 aacacactgc agcgcttgac cctgctactt ccaaaacggt gatggaattg acagaaaagc
    31501 tgatcactga gcagaatctc actgctttta tggtcactca tgacatggaa gacgcgatcc
    31561 gctatgggag tcgactgatc atgctgcatc aaggaaggat cgtcgtagat atccagcaag
    31621 aggaaaaaca gcagctcaca gtgccccaat tattagcgtt gttcaaacaa aacagcggca
    31681 cggcattaaa agacgatcaa gtacttttat cttcataatc agaaagagga tgggacaaaa
    31741 aatgaacgcc caccctcttc ttttttctct ttccttcttt cgtttttgct ttatgtatca
    31801 accgttgttt cttcgaaaac tattttgttc tctccttctt cctgatagaa tggatgggaa
    31861 aggagcttga tgccatgaca acgaaagaca aggtacttca attattgaaa aagacaccag
    31921 attttctttc cggagaaaag ttggcacagc aactggaagt ttcccggaca agtatttgga
    31981 aagccatcaa agaattggaa aaggcaggct atcgttttga gcacgggcct acagggtatc
    32041 gttacctgcc ctctgacgta ctagatgcaa aagaaatcca gcaagagcta cggtcgttta
    32101 ttcctgattt gaaagtacaa acgatgttga cttctgaatc tacgatgaaa gatgccaaac
    32161 tggcagcagc tcaaggagat tctgtttcga agttatttgt tgctgataca caagaacaag
    32221 ctcgtggacg ttttggacgg ccttttttcg ctgaacctgg acgaggaatc tacatgagtc
    32281 tcttgctcca tccagacaaa cggtttgatg aattgcctca atatacgatc atcgcagcca
    32341 gtgcctgtgt gaaagccatt gaatctgtaa ccggtcgttc tgcttcgatc aaatgggtca
    32401 atgatatcta catggatgga aaaaaagtct gtgggatttt atctgaagca gtcagtgata
    32461 tggaaagcgg caggatcagt catatcatca ttgggatcgg gcttaatttt tctgttcctc
    32521 aaaatcattt tccagctgat ctccaagaaa aagccatctc tgtctttccc gacggacaag
    32581 ctagtgtcac aaggaatcag ctaatcagca aaatctggca agaattgttc acttatcttt
    32641 ctcacccaga aaaagcgttg gacttctata aagaaaaatc attcgtattg ggaaaaaccg
    32701 tgtcgttcaa tcaaaaagga acgacttatg tcggtcttgc aaaagaaatc ggtgatcatg
    32761 gcgaactgat cgtccaaaca aatgaacaga agatgagttt gtcttctggc gaaatcagtc
    32821 tcacatctat ccgtacgcaa taattatcag atttcacaaa aggacacctg atctcataca
    32881 gtgtcctttt ttctgaattc tcttcaaaag actctcctta ttctatcagc tgacagtttc
    32941 tctatcctct agaaaaaggt attctaaaaa tatctataaa gcatggtaca ctttctttaa
    33001 atgaatcaat gcaaaaaaca agagagtcat cagtttacac gatcaaaaga gatagcaggc
    33061 cgcttccttt ttcaatctta tagatagacc atgggtaacg ataaaaacaa aaaagaactt
    33121 gcgacttatt ttgccgtaag ttctttttta gctagtgcct taatctctta atcccaacaa
    33181 acgtctcgca tagatcacct gaccagagtg catagcagca tcatcaataa tagaaacgag
    33241 acgcattcct cgtgtgactg gtggtgtcca gttctcgtca attacttcat ctagctcgga
    33301 aggttccagc cttcttaagt aagaaatagc aaaatcagca gctcctcgca agtatcctag
    33361 cagatcttct atttgattag cttggatctg ctttgcttgt tccagactat gcttccaatc
    33421 ttgttcgtta tctgctatcg tcatcggaaa ccggctttcc cagtcttgac tatgccaaat
    33481 tggagaagtt cctgctaaat cagcaatctg gaaatctaat tctcgagcgg tatgccaagc
    33541 cagccaagtc attgatttga tctgcggggc tctatcttcc actggaaaac gattggcctc
    33601 ctcaatagag acgccttcaa acaaacgttc aaaccgatcg tatgcccgat ccacactctc
    33661 gcatagcata tctaaataat ccatttattt tcctcctttt tatttatcct acttactttt
    33721 tcctaattct aggataccta cttatatgtt actacacacc cttatttttt acactttaac
    33781 agtcaccatc atcaatagtt tggagattga aagaaaaaga tagtgtcgaa ctatcaattg
    33841 aacaagaagc aacaatatga acgtttcatt aatattcttt aaataaacaa tgatttttca
    33901 aacgaacagt ttaagactag gagaaaaaga gtataatagg aaaatgtttg aaaggaaagg
    33961 aaggggaaaa atgaaaaaaa gggcaagttt actagtccta gttgctttgt tgttcaatat
    34021 cttttcgtat ggtgtggccg tttcagcgga aacatacatt cctaaagacc ctgggaatat
    34081 ttataatgat ctaactggta actatggctt gttaggaatt gctagtcaat tccacgtatt
    34141 tgctaaaaat aagacaactg tgaatgcaca tacagacgga aatatagcaa cacatgaact
    34201 tgatgcaaaa aataattttg gtacagagct tcatgaaggt ttattgaacc aagaaataaa
    34261 ttatatacaa aaaactgatt ctttcgctaa ttcttcaggc atacctacag gtagcacaag
    34321 aatgaataaa tttgtcgttg gcagtaacgt ggatgtagca cttcaagatg gtcatcctac
    34381 agtcaatgga tccaggcttg atcacttaac tccagatgat ttttataaag atcgtgctgg
    34441 gcatgaatat attgatttcg agcaggaatt tgctaaattg aatgtagcag caaatgatct
    34501 tgcaacgatc actccagcta aaacatatac tgctgcagat tttccagaca tgaacaatcg
    34561 gacaatcgat ttaactgatt tatcagatac cgggttttta ttggtaaata tcgatgcaga
    34621 agtgttaacg atgaatacgc cacttcaaat cattaatcca aatgatcaag tagttgtatt
    34681 taatgtcatc aacagttctt ctgcattgaa cgtacagtca ccaatcaaat acaatgatcg
    34741 ttcaaatcat gaaacagaag atttttcgga tgcgaatatc tcttggaatt ttggaaatga
    34801 gatgactgat ttgacaatca gtgctccttt tcaaggaaca atccttgcgc ctaatgctac
    34861 tatccgcatt actcaaaacc aagacggaac gattatagga acaaatgtga ttttagatgc
    34921 agctaccaat cgttgggatc ctaacgaaat atttatcagc gacaatggta cagatacgac
    34981 agacacaagc gacagtacag atacatctac cacaagtgat agctcagatt catcaacttc
    35041 aagcgacagt acagatacta cctctaccac aagtgatagc acggatacat cagcttcaag
    35101 cgacagtaca gatactacct ctaccacaag tgatagcacg gacacatcag cttcaagtga
    35161 cagtacagat actacctcta ccacaagtga cagcacggac acgtcagctt caagcgacag
    35221 tacagatact acctctacca caagtgatag cacggacaca tcagcttcaa gtgacagtac
    35281 agatactacc tctaccacaa gtgatagcac ggacacgtca gcttcaagcg acagtacgga
    35341 tacatctacc acaacaagtg atagcacgga cacgtcagct tcaagtgaca gtacagatac
    35401 tacctctacc acaagtgata gctcagattc atctaccact acaagtgata gcacggacac
    35461 gtcagcttca agtgacagta cagatacatc ctctaccaca agtgatagct cagattcatc
    35521 aacttcaagc gatagtacag acacgtcgtc taccacaagc gatacacgtc atgcaagtgt
    35581 ctccttctca aaaacaagtt cggataacag gacgaacttt aacttaacta ataaaacaag
    35641 caatgacaat ggaaacaaat caaaaagagc acttcctaaa actggaagtc aatcgaataa
    35701 ttggataaca ctcgcaggcg ttatattact tgtaatcggt ttaagaatca cttttatgag
    35761 ttataagaaa tcgaaaaggt aaaaaaatat tcaagataaa aaagagcaac gtcgctaacg
    35821 gacgttgctc tttatttttg ctttttatca taaatacaag aactacaatt caatttgtca
    35881 aaaaacacgt agatttcctt ctgagaaatt tatttacgta tcgaaccttt ccctattcag
    35941 tctttgcgat ccaaaatcag ttgggtaagg gaaaagagat tctcttttgt tcgaagagag
    36001 gtggcaggta atttctttat ttccttcaaa gccttttctg tgtaagaggc cgctaacttc
    36061 ctagcttctt ccacgcctcc tgtatgttca acgattttgc gtacggcgtc gacatcttct
    36121 tctgtcattg ccgctttctt ctctaaataa ggtagtagct ttggtttgtt tttttgcaaa
    36181 gagtagatca acggtaaaga atagacccct tgacgcatgt cttctagtac aggtttgccg
    36241 atttcctggc tctcttttgt ataatctaaa atatcatcga taatctgaaa ggccatcccg
    36301 atagaaagtc cgatatctcc tgcttttttt gcgaagcgct cgcttgttcc actttcaaaa
    36361 gcgccaacag aacaacttaa ttggaacagt tcagctgtct tacctgaaat attttccaaa
    36421 tattctttta cagaaagttc ataattatag cgatcatcca tctgacctaa ttcgccattc
    36481 aatacttttt ccatactgcg agaattcaat tggagacttt tcaaggaagt ggagtaatca
    36541 gacaatagtt taaaacagca gacaaataaa tagtctcctg catagacaga aacgacatta
    36601 ccaaactgac tacggatatt aggctgacca cgtcgcagat ccgcttcatc aacgatatca
    36661 tcatggatca gtgtggccgt atggagcatt tcgatagaag ctgccaaagc gatggctttg
    36721 tctcgatctt gctctggtcc aaattgtgag aacaatagct gataggcagg acgcaacagc
    36781 tttccgccgg catggatcgt ttctttgatt gcttgttcga caggtttgtt tttcaggcgg
    36841 acagtttctt ccatccgttt tagtgtttct gtcaattctt tacttaaagc aggatagttt
    36901 tcccacattg gatgtagctt cataaaaaaa ctccttgtgt gacgattatt ttttggtaaa
    36961 cattgggctc catttttgtt tggttccctg ctcataatct ttttggaatc gttgaagtgt
    37021 ctgcacatgt cgtaaaagat aattcgctgc gatcccgatt gcaatccctg ataaaatccc
    37081 taaaactgaa aggactggca agtacaacat gactgtccat gattgtgcaa tgaaagaagc
    37141 catcaaaagc tgtccgacat tatgcatgaa accgccggcc gcactaattc cgatgatact
    37201 gacacgtttt ggtcctaatt gcttgaccaa taacattcca aaataactaa gcaaagcacc
    37261 actcatacta taaaggaatg tggaaatcgt tccgcctagc aaagtagtca ggaatagtcg
    37321 tagccaaagt agtaagaagc tgtctcgttt tttcatggta aaaatggcaa cgatcgtgat
    37381 tagattggct aagcctagtt ttgcaccagg cgcaaaagaa aaaggataag ggatcatatt
    37441 ttccaataaa ccaatgatca ctccttgtgc aacaagtagc gaaatataaa tcattttttg
    37501 taatttactc atggtgtgtg aaggtctaat aaatcagact gtcttcgtca cctccatctg
    37561 ttgaacggac ttcgatcact aatttatgtg gcaaacagac gatggtttcg ccatttttcg
    37621 ttgcccagcc tcgacgtacg cagatctggt catcgcaatc tgcttctttt atccgtactc
    37681 gatcgccatc tatctcgatc aagttataat ctccgtgttc atctttgtaa gtatatgtgt
    37741 tgctctcttc tccagccatc aaagggaatg ttttgatttc tgtgccatcc acacgcagta
    37801 ccgcttcttt ttctgctgtt gcggtgtttt gctgaagacc aaaaatcatg actggtaaaa
    37861 aagaaagaac agtcaaaagc aaaataataa aaacatccca aggtttgagc agactttttt
    37921 cagcaaattc tttaaggctc atttttgttc tcctttttcc agattaactc acccaatttt
    37981 agcataaaaa caagaggttg tgacacctgt gtcttgcttt gagtaataag aacgaagaat
    38041 agaaaacagc tttccatgtt ttctaattct ttgggcttat taccgagaag cagcaggtgg
    38101 aacaccgttt ataacaagag gttgtgacag aagtgggcaa cttcaagaaa taagaaggaa
    38161 tttacgaaaa ttacttttca agtttttgtg aaatttcggc ttatttttcg aagaagctac
    38221 ttctggaaca ccgtttataa caagaggttg taacagaggt tgtgacacct gtgtcttgct
    38281 catggaggct tatctcgggc atcttattcc tttaatttta cataaaaagg aggctgtgac
    38341 ggtttgtctc agcccctttc tattgaaatc ttgcattcaa ataacgttct ttatctattt
    38401 atttcttgcc gtactgggaa cttggtctac cgtaccacca attacctagt gtacgttgta
    38461 gccatggaat tacccagcga tccaacccaa tcgcacgtcc ggaaccgttc atcaatgcga
    38521 aagcaacaaa aatgaaccaa atgtttaccc agtaaaacat gcctgataag cagaacgtga
    38581 tcaccaatac aatcgttgtc gcactgcata gccatgtgaa taatccagca atcaatgcta
    38641 atgccaaggc aatttcaaag aataccatga atttttggaa gaacattgct acatcattgt
    38701 ttggcatgat cacttgcatg atctttgtga accagtctgg tgacttctca atcacagcca
    38761 ttggttcttc accgtatgca taactcaaac caaaatgtgc agcttgagtc gctgcttctc
    38821 cggcacttgc ccctgctccg ctggcagctg tggttgcatc accagcagca gaagcaccac
    38881 tggcaacatc tgttacattg tattcccatg tccaagggaa cacactgttt ccagtgaacc
    38941 atgatccttc accaaaccaa gtacttggtt tgaacacttc accattccca atgatttttt
    39001 tggaagcttc aacgaaccaa actaaaccat agaaaatacg aagtggcaca ctccataaga
    39061 cattgccgta acgagaagaa tgtcctcgag tcacgttgcg atcatctttg atatggaaaa
    39121 actcatgcat taaatattgg aacatataat aaccagaacg aatatcaaag aagtatttca
    39181 aattcacgat atgtttcatt acgatagcta aaaatccgct taaatggatc ttatcaaata
    39241 ggttagctac cccccatttg gcaccaatag aaaccataaa tccttgatag tttcctttaa
    39301 atttatgctt ttctccgcct ttgatcgaag ccacgatatt cgctgcggct gtgtgtcctg
    39361 tttgttcagc agcttgaacg atctgcggtg tcggtgttcc tgggaattct tcataaaaga
    39421 ctaaatcacc aatgatatag atgtttttat cttcgtagcc ttttgcctgc atatattcat
    39481 tggcgatcaa ccgctgtccg cgtgctgatt ctaatccaaa gtcagcagcg tcagaagttc
    39541 ctttgacacc agctgtccaa atcagtgtgt gagtcggaat gttgcgtccg tctttcaatt
    39601 tgatatgatc agcagccact tctacgattg gtgcattcaa taataattcc acgttgtgct
    39661 tacgcaaata gcgttcagct tttgcagcat catttcgatc caacatattc aagatcgtcg
    39721 gcattgcctc tacgaccatc agtgtgattt cgtttggatc taatttataa tctttggcta
    39781 ggcgattttt ccagtcgatc agttccccaa tcatttcaat accggtaaat ccagaaccac
    39841 agacaacgaa agtcagcatg gctttacgca gttcatcatc tggttccaat gcagcttttg
    39901 ctactgtctt ctcaatatgt tcccgaatcc ttacagaatc ttcaaatgac caaagggtga
    39961 atccgtgtgc tttgacacca ggtgtaccaa agtcattcgg ctctccgccc attccaagga
    40021 tcagctgatc aaaaggatag gaacctaatt tggtcttgac gatttttcgt tctttatcga
    40081 taccgattac agtgtctgtg acaagttgta cattcttttg acgagcaaac aaatgctgca
    40141 gatcgtattg gattgcttct ggttctacac gtccgccagc tacttcatgc aactcggtca
    40201 tcatagtatg gtacgaatga cgatcgatca gagtaattgc tacatcttct tttttcagtt
    40261 tctttgccaa gtattttgta gctgaaacac cggcatatcc tgctcctaca acgacaatat
    40321 tttgctttgc cattgatcat ccgctcctta tagactatct atataatgaa tatatgataa
    40381 ttcttttagt ttgatactta ttacacaatc tccatatatt atatagatgt tctgttcttt
    40441 tgtctaactc cttttggatt tttttcaaat tttaaaataa agcgtttttt cacgaaattt
    40501 gctgaaagaa aacgtttgaa atattacatg ggttatttat caaaaatagt tgacttatag
    40561 gctaaaaaga ttagaatgta taatgaatca caaagaaaaa ttgtggacta gaaatggaat
    40621 ggtggggtat taaaatgaag aaaaaacgtt ttgtaactgg atttgccgta ttagcatttt
    40681 cagctctagt gttaggcgct tgcggcgcag atgacaacgg aagctcaaac tcttcttctg
    40741 aatcatctac agcacaatct tcgactgcaa aaagcacaac agaatcatct gcaaaagttg
    40801 tagctggcgg agatttgcaa gacggcacat ataaattaga agaaaaaaat tattcaaatg
    40861 gttaccgtgc agtctttgaa atggttgtaa aagacggcaa aatcactgaa tcaaaatatg
    40921 ataacgtcaa tgaaaacggc gaatcaaaaa caaaagatgc agaatacaac aaaaacatgg
    40981 aagcaaaatc tggcacaaac ccagaaaaat acattcctga gttgaatgac caattcttaa
    41041 aagcacaaag cgcaagcggt gtggaagtcg taactggagc tactcactca tctgaaagct
    41101 tccaaaacta cgcacaacaa ttgatccaag ctgctcaagc tggtaacaca gatacaatcg
    41161 aaatcgacaa tggtgctgat ttgaaagacg gcacttacaa gcttgaagaa aagaactact
    41221 ctaacggcta ccgtgtgcaa ttcgaaatga cagttgctgg cggcaaagta actgaatcaa
    41281 actttgatta tatcgacaaa gacggtaaat caaaacaaga tgatacagaa tataatgaaa
    41341 atatgaaagc aaaaagcggt acagaaccaa aaacttacat ccctactctg aacgacgaat
    41401 tcgtcaaagc aatgggcgaa gaagatggct cacctgctaa tgtagaagtt gttacaggtg
    41461 ctacacacag ctcacattca ttcatcatgt acgcacaaca attagtgaat gcagctgaaa
    41521 aaggcgacac tcaaacaatc gaagtagata atatcgtaac agaaaaataa tcacataatc
    41581 atactaataa aagttggagg gtgggacgat agccagatta ggcagtcgtc tcacctttca
    41641 aaatgaagag aggaaaaaga tgcggaaaaa actactattt tccttaatca cactgggcat
    41701 ctcttttatg gtgctaactg gctgcagcaa ctcagcaaat aaaacaacca ctgaaagttc
    41761 agcagctacc aagatattga aagatccata ttctgatgaa cagttcttgt tagggactta
    41821 cgttcgtatc cgtgtgtatg atgaaggcaa agaatcagca ttgaaacctg ccttcgatcg
    41881 agtaaaagaa cttggtgata aaatcacgat caatcaaaaa ggttctgaga tcgatgaagt
    41941 gaatgagcaa gctgggatca aaccagtaaa agtttctgat gacgtctaca cgttagtcaa
    42001 gcgggcctac gaatatagcc aagattctca aggcggattc gatatggcga ttggtgcaat
    42061 cacacaatta tggcgaatcg gttttgatga cgccagaaaa ccttcccaag aagaaatcga
    42121 tcaggcattg aaattagtgg attatcataa gatcgaacta aatgacaaag aaaaaactgt
    42181 ttatctaaaa gaaaaaggaa tgatcatcga tttaggtgca atcgccaaag gttacattac
    42241 ggacgaagta gtgaaagtct tgaaaaaaca aggagtgacc actgccatcg ttgacttggg
    42301 cggtaatgtc tatgtcttag gccacagccc aagaggcgaa aaccaagact ggacagtcgg
    42361 catccaagat ccgaacatgg ctcgcggatc tgttttaggt tctattaaag aacggaacaa
    42421 aacattagtt acctctggaa tttacgagcg ttatttagaa gtagatggca aaaaatacca
    42481 tcatttgttt gatccaaaaa cgggttatcc gttcgacaat gatatcgcaa gtgtcacgat
    42541 cataacagat aaatcaattg atggtgacgg gttgtctaca gcagtctttt caatgggtgt
    42601 gaaaaaagga ctggaatacg tcgaaagcga attgaataac gggacggaag cgatcttcgt
    42661 gacaaaagac gataaagtat atgtgaccga tccaatcaaa gacaccttca aattatcgga
    42721 ggactcccat tacacgatgg gtgaccgttc tgaactaaaa taaggagtac gaaccatgac
    42781 actaaaaatc tttttagaag tagttgaaat acgaacaaag gtggctagtg tatttccttt
    42841 tgcggtaggg gtattattct ctatcgcttt ttttcatgag atacattggc taaacacttt
    42901 tttatttttt attggaatgc tagtttttga tttagcgact accgctatca ataattacat
    42961 ggattatgaa aaggctcatt ctgaagtata taagtatgaa gaaaatgtca ttggccgcag
    43021 aaatctatct cctgtattga ttcgaaacat gattttagct atgattgcat ttgtttttgt
    43081 tatcggcgtt attctaacga tcaatacggg ttggctttta cttttgatgg gatttatctg
    43141 ttgttttatt ggagtattct atacattcgg accgattcct ttatctcgaa tgcctttagg
    43201 ggaaattttc agtggggtga ctatgggcct tggaattttt gccatggtcg tttatgtcaa
    43261 tacattcggt tcagatgtct atgatctgac agtcaacttt tcatctggac aatttgcttt
    43321 ggtaggaaat ctttgggcta tagcagcaat cgtattagct tccttaccct tggtgtttac
    43381 gattgccaat atcatgttgg ctaataattt gcgggatctt gaccgtgata ttgaaaatca
    43441 tcgctataca ctagtttttt atatcggacg tcctgcggga acagtcttat tccagctatt
    43501 gatgtatgct tgctatttcg tgattttaat ggggttgttg agcggtatct atgaatggcc
    43561 gatcttggtc accttcttga ccttgccaat tgtgaagaag catttggatc aattcaagca
    43621 aacgttaccc cagcctatca gtttcagcta ttccatcaaa aatatggtct tattcaatgc
    43681 cagttatgta ttggggctat tactaacaat cattatgcaa gccatttaac agcatgaaga
    43741 ggttgtgaaa aaactgccct gcatcaagta ataagaacag ccaaataaaa atagctcctt
    43801 atatttttcg tttggttggg cttattaccg cagatgcaag cttttgaaca ccgtttatca
    43861 ggaggttgtg a
//
LOCUS       DAZUKC010000016        43608 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000016 DAZUKC010000000
VERSION     DAZUKC010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.016"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(248..2629)
                     /locus_tag="ACG80T_000797"
     CDS             complement(248..2629)
                     /locus_tag="ACG80T_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFS4840469.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2619..3266)
                     /gene="recU"
                     /locus_tag="ACG80T_000798"
     CDS             complement(2619..3266)
                     /gene="recU"
                     /locus_tag="ACG80T_000798"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFS4840470.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3330..3869
                     /locus_tag="ACG80T_000799"
     CDS             3330..3869
                     /locus_tag="ACG80T_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFS4840471.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3959..4378
                     /gene="gpsB"
                     /locus_tag="ACG80T_000800"
     CDS             3959..4378
                     /gene="gpsB"
                     /locus_tag="ACG80T_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFS4840472.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4400..4767
                     /gene="rnpB"
                     /locus_tag="ACG80T_000801"
     ncRNA           4400..4767
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACG80T_000801"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4976..6142
                     /locus_tag="ACG80T_000802"
     CDS             4976..6142
                     /locus_tag="ACG80T_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFS4840473.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6188..7684
                     /locus_tag="ACG80T_000803"
     CDS             6188..7684
                     /locus_tag="ACG80T_000803"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFS4840474.1"
                     /translation="MKEKEFLQEVKEINLLQKALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     LLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7765..8448
                     /locus_tag="ACG80T_000804"
     CDS             7765..8448
                     /locus_tag="ACG80T_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS4840475.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9093..10283
                     /locus_tag="ACG80T_000805"
     CDS             9093..10283
                     /locus_tag="ACG80T_000805"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFS4840476.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10385..11338
                     /locus_tag="ACG80T_000806"
     CDS             10385..11338
                     /locus_tag="ACG80T_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFS4840477.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11407..12756
                     /locus_tag="ACG80T_000807"
     CDS             11407..12756
                     /locus_tag="ACG80T_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS4840478.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(12939..13262)
                     /locus_tag="ACG80T_000808"
     CDS             complement(12939..13262)
                     /locus_tag="ACG80T_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840479.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13391..14554
                     /locus_tag="ACG80T_000809"
     CDS             13391..14554
                     /locus_tag="ACG80T_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFS4840480.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14624..15973
                     /locus_tag="ACG80T_000810"
     CDS             14624..15973
                     /locus_tag="ACG80T_000810"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS4840481.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(16018..16449)
                     /locus_tag="ACG80T_000811"
     CDS             complement(16018..16449)
                     /locus_tag="ACG80T_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4840482.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16837..19479
                     /gene="alaS"
                     /locus_tag="ACG80T_000812"
     CDS             16837..19479
                     /gene="alaS"
                     /locus_tag="ACG80T_000812"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFS4840483.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19679..20275
                     /locus_tag="ACG80T_000813"
     CDS             19679..20275
                     /locus_tag="ACG80T_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS4840484.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20397..21545
                     /locus_tag="ACG80T_000814"
     CDS             20397..21545
                     /locus_tag="ACG80T_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS4840485.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21583..21996)
                     /locus_tag="ACG80T_000815"
     CDS             complement(21583..21996)
                     /locus_tag="ACG80T_000815"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFS4840486.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            22075..22506
                     /locus_tag="ACG80T_000816"
     CDS             22075..22506
                     /locus_tag="ACG80T_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFS4840487.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22605..22838)
                     /locus_tag="ACG80T_000817"
     CDS             complement(22605..22838)
                     /locus_tag="ACG80T_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4840488.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            23021..24691
                     /locus_tag="ACG80T_000818"
     CDS             23021..24691
                     /locus_tag="ACG80T_000818"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFS4840489.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24838..25224
                     /locus_tag="ACG80T_000819"
     CDS             24838..25224
                     /locus_tag="ACG80T_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840490.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            25211..25909
                     /locus_tag="ACG80T_000820"
     CDS             25211..25909
                     /locus_tag="ACG80T_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFS4840491.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            25914..26378
                     /gene="lspA"
                     /locus_tag="ACG80T_000821"
     CDS             25914..26378
                     /gene="lspA"
                     /locus_tag="ACG80T_000821"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFS4840492.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26375..27286
                     /locus_tag="ACG80T_000822"
     CDS             26375..27286
                     /locus_tag="ACG80T_000822"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFS4840493.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27521..28060
                     /gene="pyrR"
                     /locus_tag="ACG80T_000823"
     CDS             27521..28060
                     /gene="pyrR"
                     /locus_tag="ACG80T_000823"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFS4840494.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            28074..29357
                     /locus_tag="ACG80T_000824"
     CDS             28074..29357
                     /locus_tag="ACG80T_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFS4840495.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29370..30296
                     /locus_tag="ACG80T_000825"
     CDS             29370..30296
                     /locus_tag="ACG80T_000825"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFS4840496.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30315..31595
                     /locus_tag="ACG80T_000826"
     CDS             30315..31595
                     /locus_tag="ACG80T_000826"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFS4840497.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31600..32676
                     /locus_tag="ACG80T_000827"
     CDS             31600..32676
                     /locus_tag="ACG80T_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFS4840498.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32681..35866
                     /gene="carB"
                     /locus_tag="ACG80T_000828"
     CDS             32681..35866
                     /gene="carB"
                     /locus_tag="ACG80T_000828"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFS4840499.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            35920..36696
                     /locus_tag="ACG80T_000829"
     CDS             35920..36696
                     /locus_tag="ACG80T_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFS4840500.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36693..37625
                     /locus_tag="ACG80T_000830"
     CDS             36693..37625
                     /locus_tag="ACG80T_000830"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFS4840501.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37622..38338
                     /gene="pyrF"
                     /locus_tag="ACG80T_000831"
     CDS             37622..38338
                     /gene="pyrF"
                     /locus_tag="ACG80T_000831"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFS4840502.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38345..38974
                     /gene="pyrE"
                     /locus_tag="ACG80T_000832"
     CDS             38345..38974
                     /gene="pyrE"
                     /locus_tag="ACG80T_000832"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFS4840503.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            38962..39276
                     /locus_tag="ACG80T_000833"
     CDS             38962..39276
                     /locus_tag="ACG80T_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840504.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            39314..39955
                     /locus_tag="ACG80T_000834"
     CDS             39314..39955
                     /locus_tag="ACG80T_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFS4840505.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            39998..40690
                     /locus_tag="ACG80T_000835"
     CDS             39998..40690
                     /locus_tag="ACG80T_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFS4840506.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            40687..41589
                     /locus_tag="ACG80T_000836"
     CDS             40687..41589
                     /locus_tag="ACG80T_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4840507.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(41747..43453)
                     /gene="efbA"
                     /locus_tag="ACG80T_000837"
     CDS             complement(41747..43453)
                     /gene="efbA"
                     /locus_tag="ACG80T_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFS4840508.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        13969 a         7687 c         9477 g        12475 t
ORIGIN      
        1 actaaataaa agactagtgt gtatttagcc gatttaatta taaacaagta aatggataaa
       61 gtatatgtaa tgataaaaga aaaatggatt ttctaatttt taagctaaat gttagatcac
      121 acatgtatat gctggatagt cttataaaaa atcatgtaat acagcttggt acatgatttt
      181 tatttgtagc tgttttttac atagacacaa aaaagaaacc acgcttgtag acgtggtttc
      241 ttttttacta tctgtttcca gaagctgagg tgctgctgga tggtgtatgg tcatttgcag
      301 catccgctcc gctagttgct ggctcactgg aagaagcagg aggagcagat gaagacggtg
      361 tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga agacgctggc tcgcttgttt
      421 ctgctgtcgt gctttctttt gaggactcag cagttgattc gctttgacta gtagagctag
      481 agctttgagt cgttgtctct gttgttgagc ttccaggagt ctggacatag ctggaagatg
      541 gaatagttgt tgatggtgta attgcattcg atctagctgt atattgatct ttgtagtaca
      601 gttcaccgcc cacacgaatt aatccgcttg gcatctccca gtctgtattt gtcacgttag
      661 ctgaaacata ctgcataagc tcgcgataaa catcagaggc tacgtgtgac gattctgaag
      721 tgacaggtgt cattttttta ttgtaacctg tccacacgga aatcgaataa tttggcgtat
      781 agcccgcaaa taggatatct ggatacaccc cagaagaaat accaagtttt gcatactcgt
      841 catctgtgta attggatgtt ccggtcttac cggcttgata taacccagcg atttgcgcat
      901 tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc tgtgatcata taagctgttt
      961 ctggagacat ggctgttttt ccatctggct cataagtttc ttctgttccg tcttggaaga
     1021 cgattttatt aacatattga ggtttgtaat aagtccctcc attggcgaaa gccgcatagg
     1081 ctgctgccat ttttaaggaa gaagctccgt attttgtccc atcttgttct tccgtattac
     1141 tagagatcgc attcgattga tggatcgtac tgtattcgat cccaagattt tttaagaaag
     1201 aagccacttt atctgaaccg acttcattga ataatttgac tgctggaaca ttacgggata
     1261 agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg attatcccag tttcctacag
     1321 gagtcgaagt cccttcatag ttatacggtg cgtcagtaat agtttttcca gtagaatatt
     1381 tcaaatattc aaaagctggt ccgtaatctg tgacaggttt catcgtagaa ccaaaatcac
     1441 gggaagtatt cactgctaag ttgttgccta aagttacgtc ttctgcgatg tgacgtccgc
     1501 caatctgggc ttttactttc ccggtattgg tgtcgataag ggtggaggca acttgcatct
     1561 cgtcgtctgg gtaagagaca tactgatccg tgttgacgat atcatatagt ttcttttggg
     1621 cgtccagatc caaattcgta taaatctcta aaccgtctgt gtagacattc ttatctgttt
     1681 tttcttgtac ttcattgatg acttctttga cataattatc gacgatctta gtattgtcat
     1741 cggattgagt tagttcttgc agaccatccg ttaccggtac attaacagct tgatcatatt
     1801 ctgtttgaga gattttttcg ttttgaagca tcgtatacaa aacagtatct cgtcttttct
     1861 tcgcctgatc tggataaaca taaggatcat aggcggaagg ggcttgaggc atcccagcaa
     1921 gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt accaaagtac atctcagaag
     1981 ctgtctccat cccgtacaag ccgtttgaca tatacacttt atttacgtag taggtcaaga
     2041 tctcttgttt cgattttttt tgttccaatc ttacagccat ccacgcttct tgggccttgc
     2101 gtttcagagt ctgatcctcg gcactggtgg agaagaagga aagtttgatc aattgctgtg
     2161 tcaaagtact tccgccttgc agtccgccag aggtaaagtt agagagggca gaaccgatga
     2221 tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg atcttctacc gacacgatcg
     2281 catcttctaa tgttttcggt aattcattcg cagaaatttt ttcacgcttt tctgcgccta
     2341 aatcttcgaa tagttcgcca tcttgtgtat atagtttgga agatacggta gcgtctaatt
     2401 ttttatccgt caattcgggc gcatcttttg cgtaatacca gaaaagtcct acacctgcta
     2461 aaaagaggat acagaaaaat gagagtattc ctaaaaggat cttgataaat aatccttttt
     2521 tatgtgttcc agaggacttt ccagaacctt tgccagaatt cttcttcaca ctctttttag
     2581 gagtaggctg tttctgtcgt ctagagcttc tagtttgttc atttgccatt cttgctttct
     2641 ccttcaatag ataatgttga taaatagaag tcaaccgcgt ccaaataagg aatccgcgga
     2701 gaaataccag ttttgatctc tattcctagt tcttcgatca aagataaagg tagtgatttg
     2761 cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat gaacactatt caaataaaaa
     2821 cagcgcttca aagacgaaaa ccataacaag acaaagcaga ccccattttg ttcgaggcat
     2881 gctttcatat gttcgatttg atgggcatgg aagtttttaa agggaaaaga tgttttgttc
     2941 ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat agacgccatt atagtctgta
     3001 gttgaggctt gggtaaagta ggcttccttg atcacagcag cacttctctt aggatagtct
     3061 actttcacga tttggatagg ggtaggcttt ttatggataa ccgccaaccc gcgggaaaga
     3121 taatatttat tactttcatt gattgcttct tcaaagcgca tcccccgatt gccgaattca
     3181 gtgatctgat ttcttttttc ttgaattttt tcgacgggag aggcattctt ataataaggt
     3241 tggccatttg gatagcgcaa taccatcttt aatcacactc ctaggccaca ttatagcaaa
     3301 aaagcggata gaaagaaaag gaatctgaga tggataagtc aaaagtaatc tatgtgtctg
     3361 ggtatcgaag tttcgaactc ggtgttttta aagaaaatga tcctaaaatc caagttgtta
     3421 aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga agggctggaa tgggtacttg
     3481 tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat agtagcggaa ttgaaagaag
     3541 aatatccgga actatctctt ggtattttgt atcctttttc tgattttggt aataactgga
     3601 atgaagggaa ccaagaaaaa aaacaattag cagaacaatt agcagattat gtcgaagctg
     3661 ttagccatca accctaccaa tcacctagtc agctaaagaa tcacactcgt tttatacttg
     3721 agcatacagt gggctgtctg ctgatctatg atgaagaatt ccctggaaaa acgaagtatt
     3781 tttttgaaga tgcacagaat taccaagcag atcatcccta cgaaattcgg ctgatcagta
     3841 tggacgatct tcaaaatttt agtgaataat cgatattgtg tttgatttat caactttttt
     3901 cctatacaat gaaaaaggta gaaatgttat tacgtaaatg aaaactagag gtgtaaatat
     3961 ggcgaatttg gtttatagtc ctaaagacat tttacaacaa gaattcaaaa caaaaatgcg
     4021 cggctatgat cccgttgaag ttgatgaatt cttagacaat atcatcaaag attatgaaac
     4081 ttacagcaaa gaactccttg ctttacaaga agaaaacgat cgattgagtg caaaagtagc
     4141 tcaattatct aaaactcaag gagcagctca aacacgcgtg caacaaacag aggcaccaaa
     4201 gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg tcaaatcttg aacgtgaagt
     4261 atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa ccagcacaac ctaatccaaa
     4321 taactacaca aatgcagaca caagcttaga tgataatgaa aaaacacgtc aattttaatt
     4381 tttgattaat gaatttgcgg ttttcgggta atcgcggtct gacttgatca ggctgaggaa
     4441 agtccatgct cgcacaggct gtgatgcctg tagtgttcgt gcttagcgaa accataagct
     4501 aaggtactct ttggagtaac ggcaggaaaa caagctaagg cttcggctat gcttaagtat
     4561 ccttgaaagt gccacagtga cgaagcggct ggggaaaccc atccggtgga acgcggtaaa
     4621 cccctcgagc gagcaaccca aacaatggta ggggcgcttt ttgtctggaa gaagaacagg
     4681 acaaaaaggc agtataactg cagatagatg gttaccgctt tgttttttct ccctggaaaa
     4741 gcaaagtaca gaacatggct tatagaaaac cgcaaacatc agggaattag gtcgtgacaa
     4801 aagtaatgcg ttataagaaa caagaaaaac atttgaaagc tatttttcaa tacttcagat
     4861 gaatcgttct tattacaaaa caaacttttg tatcaccggt tattcggaat ttgagtaaaa
     4921 gtgtggggta ggactgatgt cccacctcct ttttttataa ataaggagat tgtgtatgga
     4981 aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc gggctagaag cactggttgg
     5041 taaagaatta agagatcttg gaatcgaatg ccaagttgag aacggacgtg cacgttttga
     5101 aggaacaatg gaaacgatcg ctacagctaa tttatggtta cgtacagcag atcgagtaaa
     5161 aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa ttgtttgaaa acgtaaaagc
     5221 attgccttgg gaagattttc ttccactaga tgcagctttt cctgtagctg gaaaatcgat
     5281 caagtcgaaa ctatatagca cacctgattg ccaagcgatc acaaaaaaag caatcgttga
     5341 acgtctccgt acctattatc accgtccagc ttctgtacca ctgacggaaa ctggcgcaca
     5401 ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg gtaactctgg atacgacagg
     5461 accaagttta ttcaaacgtg gttatcgctt agaaaaaggt ggagcaccat tgaaagaaaa
     5521 catggcagca gcacttatta tgttgacaaa ttggcgaaaa gaccgtccat tttatgatcc
     5581 tgtatgtggt tcaggtacgc tttgtattga agcagcctta ataggccata atatcgcacc
     5641 aggattcaat cgtgagtttg cttgcgaatc ttgggattgg ttcagtcaag aaatcatgga
     5701 aaatgttcga tcagcagccg aagaaaaagc agattatgat attgaattag atattactgg
     5761 ttcagatatc aatggacgga tgattgaaat tgccaaagcc aatgcagaag aaatcggatt
     5821 aggctcttcg attaccttta agcaacaagc agtcaaagac ttcaaaacgg ataaagaata
     5881 cggggtcatc gtggctaatc cgccttacgg agaacgtcta ggagaagaag aaactgttcg
     5941 gaaattatat aaagaaatgg gcgatgtatt ccgtccatta aaaacatgga gtaaatatat
     6001 cttgactagc gacttagcgt ttgaggaatt ttacggacaa aaagcaacca aaaaaagaaa
     6061 attgtacaat ggtgcattgc gtacggatct attccagtat tggggaactc gtccgcctag
     6121 aaaaaaagag aaagacgaat aatcttacta attgattcat tcaattggat gtttggagga
     6181 atagggaatg aaagaaaaag agtttttaca agaagtaaaa gaaatcaatc ttctacaaaa
     6241 agctttagga atcctcgatt gggatacaca aacaggtatg cctgaaaaag ctagtgcata
     6301 ccgaagtgaa gtagacagct atctttacag catttatttt tcaaaaaaaa tcggtccaaa
     6361 aatcaaagaa gccattcgct atttttctga acatccggaa gaattgtcgg aagttggaac
     6421 tgccgttttt gccaaagtaa aagaagagta tgatttggaa catcgcgtac cggaagatct
     6481 tatgagaaaa tatacagctg ccgtttcttc agcacacgga caatggcaaa aagctagaga
     6541 aacacaacaa ttttctgatt ttcaacacgc attgaatcaa aatattgaat taacaaaaca
     6601 gttgatacct tactggagaa aagatgaaaa gacgaattat gatgttttgc tgaatcaata
     6661 cgaacccggg atgactgtcg aaatccttga taaggtattt gatcagctta aagaaggaat
     6721 tctttcgatc cgccgtacgc tccaagaaaa agggactgaa cctgaaacag actttttaaa
     6781 tcggagaatg acgaaaaaac aacagaagag atttgtagtg aaagtaatcg aacaacttgg
     6841 ttatgatttt agtcgtggtc gtttagatga cactgtccac ccatttatga ctggaattaa
     6901 tccgaatgat gcacgaatca cgacacgatg gaacgaaaca gatttcaaaa tggctgtttt
     6961 tggagtgatc cacgaggctg gtcatggtat gtatgaacaa gacatcgatg ataaatatgc
     7021 gtatacaccg gtttatgaag gtacatcaat gggtatccat gaatcacaat cgttgtttaa
     7081 cgagatcatt attggaagta gtcgcagctt ctggaaaaag caatatcctt tctttcaaga
     7141 atgtgcagaa ggaacattcg atgatattcc ttttgacaaa ttttatcgtt tattgaacca
     7201 tacaaaatca agtttggttc gtatcgaggc agacagtttg acttaccctc tccatatcat
     7261 cattcgttat gaaatcgaaa aaatgatttt caatgaaggg gtagatgttg ctgaactacc
     7321 tgaaatatgg aatcaaaaat atgaagagta tctgggaata cgaccaacaa atgatgtgga
     7381 aggcatccta caagacgttc attggtcagg tgcaagcttt ggttattttc cctcttatgc
     7441 attagggtac atgtatgcgg cacaattact tcattctttg cagcaagaaa tagatgtgga
     7501 aaatgttcta gcgtcagacg attattctcc aatcaaaaac tggatgtcag agcatatcca
     7561 tcaatatggt gcatctagaa aaccaaatca attaatcatg gatgcaacac atgaaccgct
     7621 taatccgcaa tacctgatcg actatatgaa agacctgtat tttaatgtat atcaggtaaa
     7681 ataaaataca atttacaaag atagctgaaa ttcatctaat ctatagatgt tttcagctat
     7741 ttacttaaga aagaagtgga acgaatggat aaattacaag tgatcgctga ttatagtatg
     7801 gaccaattag cgcatgatca aacaggacat gggagcgatc ataccaaaag agttgtcaag
     7861 ttagctgaaa aaattttgga gacggaaccg caggcagacc gctttgtgac gcttgcagca
     7921 gcttacctac atgatacgat tgatgataaa gtcgttaaaa atgagaacga ggcaaagcaa
     7981 cagcttcgtg tattccttcg ctctttgcca atagtagagg aacagatctc gatgattttt
     8041 tccattatcg aaaatatgtc cttttctaaa aatctctctg aagcggccga actatctctg
     8101 gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat taggagcaat cggcattttg
     8161 cgtactgctt atttcggcgg tggacatggg cacccgattt ttgatcctga actgtatccg
     8221 caaacattca aggacaaaga aaattaccga aaaggcacga cagtgatcaa tcatttttat
     8281 gaaaaactgt tcttgctgtc tgataaaatg aatactgatt acggatatga agaagccaaa
     8341 cgaagagaaa ccttcatgcg tgaattctta gaagaatttt ttctggaatg gtctattgac
     8401 gatgaaaatt ttactccaga caattggatc aaaaataaca aaaaatgatg gaggttgtga
     8461 cagaagtggt cagctccaag atatttcgga tcattttccg taaaagttac ttctggaaca
     8521 ctgtttacca agaggtcgtg agcctgccgt tcagctccga gtcacaagga acaatttcaa
     8581 aaaatagctt ctcgtgtttg atgaatattg taacttgtga ttcggaattg gcaggagaac
     8641 accgtttatt cggaagaata ggactgtgac aagacttttg tcacagtcct tttttcgaat
     8701 tcaacaagta aaacttttaa gaaatattca gagagtaaaa ttaaaaattg ctgataaacg
     8761 aagatggaaa ttttatgtat ttttattggt ctatgcaata aataaattaa atattcagaa
     8821 tacaattgat tttaacgaaa gggcttctat caatggttgt gttgtattta tctgcccagg
     8881 tagagtaaaa tagatcatta tctaaaaggt aattgttcat actatataag tcatttgaaa
     8941 gtaaaaagat actgtaaata aaaaatgtgg ttaaataatt ctcataatca gtgtaagcgt
     9001 tatcaaaaat atagcatact tgctttgtag atgaatgaca cgatatatgt ttggtatgtt
     9061 ctgcaagaca gatgaatatg gaggtggaga caatgtcttc caaagaacta acaatgtatt
     9121 tagagaaaga attggatcaa ctgaaagaaa aggggttata caatacgatc gacatactag
     9181 aaagtgaaaa tggtgcttgt atcatcgtag acggaaaaaa gatgatcaat ctagcatcca
     9241 acaattatct tggatttgcc aatcgcgaag aattgaaaaa agcatgtatt gaagcaaccg
     9301 aaacttatgg tgtaggagca ggagcagtcc gaacgatcaa tggttcgctg aaaatccatc
     9361 agcaattaga agaaaaaatc gcagaattca aagggacaga agcagcaatc gcctttcaat
     9421 ctggattcaa ctgcaatatg ggcgctatct ctgcagtaat gacaaaagaa gatgctatct
     9481 tatcggatga attaaaccat gcatctatta ttgacggttg ccgcttatcc ggagcaaaaa
     9541 tcatccgaat taaacatcaa gatatgaaag accttgagaa aaaagcaaag gaagcgatag
     9601 aaagtaagaa atataaaaaa atcatgtata tcacagatgg tgttttctcc atggatggag
     9661 acattgcacg attgccggaa attattccga ttgttgaaaa atatggactg atcacctacg
     9721 tcgatgatgc gcacggctca ggcgtaactg gaaaaggtgc aggaaccgtc aagcattttg
     9781 gattaagcga caaaatcgat atgcaaatgg gcacgttatc gaaagctata ggtgttgttg
     9841 ggggttatgt agctggatcg aaaacactaa tcgactggtt gaaagctaga agtcggccat
     9901 tcctattttc tacttcgcta actccaggtg cagctgcagc tgcattggct tcgattactt
     9961 tgatgcaaga acatccggaa cttgtcgaaa aagtttggga aaatgctaat tattttaaag
    10021 aagaattgaa aaaagtaggc tataacatcg gtatgtctga aacacctatt actccagtca
    10081 ttttgggaga cgaaaaagtg actcagactt tctctaagaa gctgatcgaa catgggattt
    10141 atgctaaacc aatcgtttat ccaactgtgc ctcttgggac aggcaggatc agaaatatgc
    10201 cgactgcgga acatacgagg gaaatgcttg atgaagcagt agcagtttat caaaaaatag
    10261 gaaaagaaat ggaaattatt taactaaatt tagttttttc gtctatttaa aattagagaa
    10321 catgttcacg catatattgt taatcatctg aagatagtag taaaaaagat cagggaggat
    10381 catcatgaaa aaagtattgg tgacaggatg tttaggacaa attggttcag agttgatcac
    10441 acggctacgt caagaactag gcagtgaaaa tgtcattgcg acagatatac gcatgcctga
    10501 agtcattgag aaaaacgata tctttgagat cctagatgta atggattacg aaaaaatgcg
    10561 cagcatagca gaacaatatc aagtggacac attgattcat ttggctgctc ttttgtcagc
    10621 tgttgcagaa gcaaaaccac aattagcctg gaatttgaac gtgactgggc tggtcaatgc
    10681 attggaagtc gctagagaat tccagctgaa attttttacg ccaagttcaa tcggcgcgtt
    10741 tggtccaaat actccaaaag atcatacccc acaagatact cttcagcgtc ctacaaccat
    10801 gtatggggta acgaaggtag ctggagaact actatgtgat tattattatg tgaaatatgg
    10861 cgtagatact cgtggtgtcc gtttccctgg attgatttct tatgcaacct tgccaggagg
    10921 cggtacgaca gactacgcag tcgatattta ttattcagca atcaaagaag gaaagtacac
    10981 tagtttcatt gatcaaggta catcaatgga tatgatgtat atgccagatg cgatcgatgc
    11041 cattataaaa ctcatgaatg cagatcctga tcgattggtt catcgaaatg ccttcaatat
    11101 atcggcgatg tcttttgacc cggaagagat caaagcatct attcaaaaaa tcataccaga
    11161 ttttgaaatg gattatgatg tggatcctgt acgtcagtca atagcagaat cttggccaaa
    11221 ttcactagat gtttcttgtg cgcaagagga atggggcttt tcgccgaaat acgacttaga
    11281 tgtgatgaca aaggatatgt tggaaaaact gaaagaaaaa ttggtagaga cggtataaaa
    11341 caagaaccac atccaaaaag ttttttatac ttttgggatt ggctatggta tgaaagggga
    11401 caagggatgg aagaatatca agccacgcca gtaaaagaag taaaaataga aaatgaatta
    11461 aaacgtacga tgggattttt tactgcttta tcgactgtta tgggcacagt gatcggtgca
    11521 ggggtatttt ttaaagccgc aagcgtagca gaagttacag gatccgccag tttgcatatg
    11581 ttttcttggt ttctcggtgg tatgatttct gtctgtgcag gactaacagg agctgaactg
    11641 gcagctgcta ttccagaaac aggagggatg attaaatata tcgaacggat ttacggaaat
    11701 acagcagctt ttttattggg ctgggcgcaa gtcgtgattt attttccagc aaatgtagca
    11761 gccttgtcga tcattttcgg tacacagttc gtcaatctat ttggtctttc ccagtcaatg
    11821 attgttcccg tagctgttac agctgctgta tcgatcctgt tgatcaattt tttaggttct
    11881 aaagctggag gggcttttca gtcgattacg cttgtctgca agcttattcc tttgtttgtg
    11941 atcgtcattt tcggtttgtt ccgacaagaa ggagtagact tccaactgtt tccgatccaa
    12001 gcaggagaaa atctttcatt tttctcagcc ttgggagcgg gcttgctagc aacgatgttc
    12061 gcatacgacg gttggataca cgtaggaaat atttcgggag aattgaaaaa gccggcgaaa
    12121 gatttgccaa aagcaatttc tttaggaatc atcggaatca tgatcgttta tttattagta
    12181 aatgcggtgt tcttaagaac cgcatctatc gacggagtcg caggaaacag caatgcggca
    12241 agtgacgttg caaaaatgat ttttggcggc ttcggtggaa gactggtcac agttggtatc
    12301 ttgatttctg tttatggaac catcaatggc tataccctta ctggaatgcg tctcccttat
    12361 gttatggcaa aagaaaacaa cctgcctttt agcaagctct ttgctaaact tcatgataaa
    12421 actaaagttc ctgtagccgc aggtatctta gagctagtca tcgctattgg catgatgatg
    12481 atcggtggtt ttgatacact aacggatatg ctgatctttg tcatttggat tttttatacg
    12541 atggtctttg ttggtgtcat tcttcttcgt aaaaaagaac ctgatttgtt tcgaccatac
    12601 aaagttccaa tgtatccgtt tattccacta gtagcaatca ttggagggac ttttatcgta
    12661 tcaagcacgc ttatcactca gaccttttta gcttcgatgg ggatagtatt gacacttgct
    12721 ggtatcccga tttatctcta tttaaaacgt agatagctaa aacattcgaa ttttattaga
    12781 actgcacttc tctgccaaac aaaatatttt atttaaatga ggagtacgtg gaagaaatgt
    12841 caaaagtact cgttaatttt gatcacgtaa atgtcgtttt gtctacgatg aaatagcaaa
    12901 gtaaaagaca gaaatcgggc tcaaccgatt tctgtctttt actttgattt gcgtaactga
    12961 actattcggc gtacgccgcg ataaatattt aataaaacca agcaaccaga aatgaaaatg
    13021 ggaagccaaa tcagatagac agggtagcca agcactttag acaaagcatg tggagtcatt
    13081 ttatccattt caagaagtgc ttgctgccaa gtctgaagat tgaagactag catgaaaccc
    13141 gaaataatca ggagaataaa tcctaaataa tgaattgtag ttctttttct tacataagcg
    13201 atcgtagcat aaaggaacag taaaaaaata ggtacgccaa aaacaaagta catattcagc
    13261 atctcgacac cccctttgtg tttatcttac tttactttga aagaaatgag aaataatagc
    13321 ctcgttactt gcttgaaaca gcaaaggact gctaagataa gagagataca aagaatcaag
    13381 gaggagtcat atggcaagtt atatcgaacc gattcgtttt gcgctgattg tttttccatt
    13441 tttagcctta gcgatctcat ctgttttttt tatttatgaa tatcgaaagt atggaacatt
    13501 tctagtaaca agggcagtta ttttgtattc tttcgttttt tatttattgt gtgcgtattt
    13561 tttggtgatc ttaccacttc cggcaaagga gacggttgcc caaatgacag ggccaaagat
    13621 ggagctgcat ttatttgctt catggcaaca tttcagagac aacacggtat tggtcttgtc
    13681 tgaacctagt acctacctgc cggcaatgaa gcagagtgta tttttggaac cagcttttaa
    13741 tgttttatta ctcataccat ttggcattta tttaagatat tatttccggc tttcttttat
    13801 gaaaacgatg attgctagtt tctgcctttc tctatttttt gaattgacgc agctaagtgg
    13861 actttatttt atctacccaa gaccataccg cttatttgat gtgaatgatt tagtgacaaa
    13921 tacattgggt ggaatcattg gttttgtcgt tacgccgtta tttacattta tgcttcctac
    13981 aagaagacga atggatgaac tttcttatga aaaaggaaag actgtctcat tgactcgacg
    14041 cttggtcgct tatttgatcg actggacatt tttatctctt attaatttgg tagcggcgat
    14101 tttctttaaa attgcgttcg attcaggggc agttacttct catatctggt ggcttttatt
    14161 agaagtaggg gtatacttta tgattgtacc gtttataacc aatggatgga cacttggcaa
    14221 aaaaattgtg cgtatccgtt tggtggaaga agaccgcgaa cgaatttctt taaaagcatt
    14281 gatgatccgc tacgggtatc tgtactttct tttttatggt ttaagctggt tggggcagtt
    14341 gcttcagcaa atcatgaatt cgacaacgaa tacggtgttg cagatgagtg cagtattcat
    14401 ttctctcggt gtcttgttac tacaaggtat ttttatttta aatatcttgc tatcattttt
    14461 gagaaaaaaa cggattctgt tttatgaaaa agctagtcac acatatactg ttagcacgat
    14521 tggccaaaca aaggatgatg attctcgaaa ttagctgagc aaaaacgctt tgtctgtgtt
    14581 ataatgccaa agtcgtgata atttaaagag aagaggtgca cgaatgaata attttgatat
    14641 attccgcttc aaaccgttta tcaatgaagc gttaaccgca aagggcttca aacagcccac
    14701 agaggtccag gaacgtttga ttcctttaat tgcaaaagga aaaaatatca ttgggcaatc
    14761 tcaaactggt acaggaaaaa cacatacgtt tttgttgcct ttgtttgaaa aaatcaatgc
    14821 agaaaaagag gaagtacaag ttgttattac agctccaagt agggaactag ctgcacagat
    14881 ctatgaagca gctacacaaa tcgctaaatt ttctacaccg gagatccgtg tctcgaattt
    14941 tgtcggcggt acagacaaac agcgtcaatt aaatcgtctg catcaccaac agccacaggt
    15001 tgtagtaggc acgcctggta gaatattaga catgatgaat gaacaagcgc tgaagattca
    15061 caccgcattt gcatttgttg tcgatgaagc ggatatgaca ttggatatgg gttttttaaa
    15121 tgaagtagac caaattgcaa gtcgattacc agaaaagctg cagtttttag ttttttctgc
    15181 cactattcca gaaaaacttc gtccattctt acgaaaatac atggaaaatc caatcattga
    15241 agaaatcaaa ccaaaagcag ttatttctga agacattgaa aattggctga tttcaacaaa
    15301 aggtaaagat gagaatcaaa tgatttatca actgctgaca attggtcatc cttatttagc
    15361 aatcgtattt gccaatacaa aacagcatgt ggatgaaatt gccgattact tgaaaaacca
    15421 aggcttgaaa gtagcaaaga ttcatggaga catcacccca agagagagaa aacgagtgat
    15481 gcgacaagta cagaatctgg agtatcaata tgttgtagcg actgatttag ctgctagagg
    15541 gatcgatatc gaaggtgtct ctcatgttat taatgcagaa attccagaag atctagactt
    15601 ctttatccat cgtgtcggac gtactggacg taatggctta aaaggaacgg ccattacgtt
    15661 atattcgcca aatgatgagc aagccattga tgcgatagaa aaattgggcg tctcatttga
    15721 accaaaagaa atcaaaaacg gggaaatcgt caaaacatac gatcgtaata gacgtgccaa
    15781 acgagaaaaa tcaaaagatg tattagatcc cacgctgatt ggtcttgtga agaagaaaaa
    15841 gaaaaagatc aagcctggtt ataagaaaaa aatcgattgg gcaattgcag aaaaaaataa
    15901 aaaagaacgc aaaatcgaac gcagacagca aagccgtact gctcgtaaaa ataagaaaaa
    15961 ttcacataaa taaaaaacaa gaggttgtga caaaaggcgt gtcacaatct cttgttttta
    16021 agctagactt tttttcattc cgatacaaag tacttcatct tcaataaaag gttctgtgca
    16081 agtatgatac ccttgttttt cataaaaatc tacagccgtc atttcagcta ctacataact
    16141 agtctgacaa ccttctgaaa ccgcccgttt ttcagcagct tcaagcaact gaccgcctaa
    16201 cccttttcct cggtattctt ttgctacgca aaatcgttcc ggcttgaagc aagtaggtgt
    16261 tttcttttca taacgcaaag tagctactgg tcttttttga tcaagaaata gataataggt
    16321 ttgttgcggg atatcttttc cgtcgaattc tagttctgca ggaatccctt gttctaatac
    16381 aaaaacttga tatctcaaaa agcagctagc tgcgcgtatc cattcatccg ctccaactat
    16441 ttttttcatc atattcctcc ttgtcatgaa tagcagagat gtattgattc ctttattctt
    16501 atcgtaccaa aaatcgtgga gtatgaccaa aaggaaatga aaataaaaat tgagatattg
    16561 acagtgatca atagaatgcg tatactaatt aagtacgaaa aggatatgat ttgtttttta
    16621 agactcacag aaaattcttt cttggctgaa aaaagaatag tcactaatca aattgctccc
    16681 tttttctatt ttggtagaaa taccgacgca gctcagcgtt atcgagttta agaggtaatg
    16741 attacacatc attgcaatca aggtggtacc gcgagttttc gtccttggct gtgatggtgt
    16801 ttttttgtat ttaatttata gaaagcaggt aatgacatga aacaattaag tagtgcacaa
    16861 gttcgacaaa tgtttcttga tttcttccaa tctaaaggac atacgattga acctagtgcg
    16921 tctttgatcc ccgtgaacga tccaacctta ttgtggatca actctggtgt tgcaacattg
    16981 aaaaaatatt ttgatggttc agttgtgcca gaaaatccaa gaatcacaaa tgcccaaaaa
    17041 tctattcgaa caaatgatat cgaaaatgtc ggtaaaacag cacggcacca tacgatgttt
    17101 gagatgctgg gtaatttttc gattggtgac tatttcaaaa atgaagccat ccactgggca
    17161 tgggaatttt tgactgatcc aaaatggatg gcattcgatc cagaaaaact ttatgtaaca
    17221 gtttatccaa aagatacaga agcaaaacgc atctggcatg aagaagttgg cctgccacta
    17281 gatcacatta ttgatgtaga agacaacttc tgggatatcg gtgcaggccc atgtggtccg
    17341 gatacggaaa ttttctatga ccgtggagaa gcttataacg atttagcaga agacgatcct
    17401 gaaaactatc caggaggaga aaatgaacgt tatttagaaa tctggaactt agtattttca
    17461 gaattcaacc ataagcctga taacacattt gaaccacttc ctcataaaaa cattgatacg
    17521 gggatgggat tggaacgcat ggtctcaatc gttcaagatg cgtcaacgaa ctttgagaca
    17581 gacttgttta tgcctattat ccacgccgta gaaaagctaa gcaaacaagt aaaatatggt
    17641 gaaaacccag taacagatat ttcttacaaa gtaatcgcag atcatattcg cgccctttca
    17701 tttgctattg gcgacggtgc attgccatcg aatgaaggga gagggtatgt cttacgccgt
    17761 ttgcttcgtc gtgctgtcat gcatgggaaa aaattaggta ttgaacaagc attcttatat
    17821 cgtttagtcc ctgttgttgg ggaaatcatg gtaagttatt atcctgaagt acttcaacaa
    17881 aaagaattta ttgaaaaagt tatccgtaca gaagaagaac gtttcaatga aacaatcaat
    17941 gaaggtttgg aaatcttaaa tcaagtgatc caagaagtaa aaaacgaaaa cgggacgact
    18001 ctagcaggaa aagacatctt caaactatac gacacatatg gcttcccggt agaattaacg
    18061 gaagaggtgg cagaagatga aggactgaaa gttgatcatg ctggttttga aaaagaaatg
    18121 gatgcacaac gcgaacgtgc tcgctctgcc cgcagtactg aaaaatcaat gggggtacaa
    18181 tctgctttat tgacagatat caaagtagaa agtaaattca ttggatacac agaacaagaa
    18241 tcagatagtc aattattggt catcgttaaa ggtgagtctc ttttgaatga agtcgcagaa
    18301 ggtgaagctc aaatgatctt tgatcaaaca cctttctatg cagaaatggg tgggcaagta
    18361 gcggatcaag gatggatcat gaatgaagta ggcgaaatcg tagcagttgt tacagatgtt
    18421 caaaaagcgc ctaatggtca atttatgcac accgtagaag taaaagcacc aatgagtgaa
    18481 ggacaaacct atcatctatt gatcgatcaa tcgatgcgca atcggatcat caaaaaccat
    18541 acagcaaccc atctactgca caaggcattg aaagaagtac ttggcgagca tgcaaatcaa
    18601 gctggttctt tagtcgctcc tggacattta cgattcgact ttacccactt tggtcaaatc
    18661 acttcagatg aattagctga aatggaaaga atcgtcaacg aaaaaatctg ggaagctatc
    18721 ccagttgaaa caattgaaac agatatcgat acagccaaaa acatgggtgc aatggctttg
    18781 tttggtgaaa aatacggaca tgacgttcga gtagtaaata ttggcggctg gtcaatcgaa
    18841 ctgtgcggag gcacacatgt agcaaataca gaaaacatcg gaatcttcaa aatcgtttct
    18901 gaatcaggaa ttggtgcagg cgtgcgacga atcgaggctg taacaagtaa agaagcgtac
    18961 gaactacttg aagaagaaga aaaacaacta aaacaagtag caacaattgt taaatcacct
    19021 caattgaaag aagttgtttc aaaagcggaa caattgcaac agcaagtaag agagctacaa
    19081 aaggaaaatg aacaattagc tagcaagtta gcgaatcaac aagctggtga tgtattcaaa
    19141 gatccagaaa cgatcaatgg aaaaacaatc attgcagcac aagtaaaagt gaaagatatg
    19201 aatcagctgc gtcaattagc tgaccaatgg aaacaaaaaa atatttcaga tattcttgtt
    19261 ttagcaaacg aacaagaagg aaaagtcagt ctgctagcgg caatgacgaa agaatcaaat
    19321 gaagctggac taaaagctgg cgacttgatt aaagcaatcg caccgaaagt aggcggtggc
    19381 ggtggtggac gtccagacat ggcacaagct ggcgggaaaa atcctgctgg tattccagat
    19441 gcacttaaag ctgcgaaaga atgggttgcg caacaatagg gcgtttacgt ttgacagaaa
    19501 aacaaaagtt caaacattga acgaaacatg attatggggg cgaagacaaa caagtcttgc
    19561 cctcatactt ttttcaattg gatggcgcat ctaatcaaat ggtcggattc ggatggtctt
    19621 tagttgacga aagtgacaga aatttttacg attagtataa ataaaaaggg gtgaaaaaat
    19681 gaatagtaga aataagacct ttcgactagt cttacgtgcg atcttgttag caatcatcat
    19741 cgtacaagcg atggttcctt ggctggggtt tattccatta ggattcatta gtttaacaat
    19801 tatccatatc acagtaatta ttgctgcagt agtattaggc ccaaaagatg gcatggtgat
    19861 tggcttgttt tggggaatcg ctacaattgt tcgtgcctat gccatgccaa caacgccatt
    19921 tgatacatta gtgttcacga atccgattat ctcggtagtt cccagagtac ttgttggctt
    19981 agtagcaggc ttggttttcc actggattta tcaaaaaaat aaatcaataa cgatcagctc
    20041 agcctttgca ggggtactgg gttcattggt caatacagtc ttagtgttag gattcatggg
    20101 attattttat acaggtgcaa cggcagatgc ctatggtgta gactctgctc ttttatttaa
    20161 aaccttagca ggggttgcag caatcaatgg gataccagaa gcaataggtg cgggaatcat
    20221 aacaccatta ctagcaaaag cgttatttgc agcaacaccg ttgaaaccag aataatgacc
    20281 atcttttaat agtaatagga cgcacgatag atagaaaaag gaagagagga gaggaaaaca
    20341 atcgtcctct ccatcttttt acctgagcaa caagcaaact agaaggggta aaaaacttgg
    20401 ctaagaaaca agaagaaaaa aagaaacaaa taccgcaaca gcggacaaaa aaaggagtac
    20461 tagcgggtat cttaggattg gttacgaatc tgatcttgtt tgtctctaag ctaatgatcg
    20521 ggcttttttc aggaagtgtc tccattatgg ctgatgcagt gaacagtcta tcagatactg
    20581 cttcatccat attgactttg atcggattta aaattgctgc aaagccagca gatcaggaac
    20641 atccttatgg acacgaacgc ttcgaatata tcagtgggtt atttgtttcg atcatcatta
    20701 cttatgtagg atttcaattt ttagagtctt ctgctgaaaa aatcatccat ccagaaaatg
    20761 tctctttaac acccatcgtc ttttttgtcc taatcatttc catctttttg aaatttgcac
    20821 aaggcaaaat gtatcaggtt attgcgcaat ccattgattc tgaaacattg agagctacag
    20881 caacagatag ttataatgat gtctttacta cactggctgt attgctttca gcagcaatcg
    20941 aatgggtaac tggatggcgt attgatggat atataggttt tgctttagca ttatatatcc
    21001 tttatagtgg attgacgatg cttcgaaatt ttgtatacga actattagga agtcgcccaa
    21061 cgccagcaga gattgaagaa atggaagagc atttaaatgc ttgcgatgat atacttggct
    21121 atcatgattt attggtgcat aactatggac ccaataaacg ctttgcatca gtccacatcg
    21181 aagtagatga atcaatgaat ttgaatgtgg ctcataagat tattgatctg atagaaaaag
    21241 actttaaaaa gtctttggat gtggaacttg tctgtcactt ggatccaatg cctgttgata
    21301 acgaaaaata ctataatatc ttgcggcagt taaagcaaat tcttcttgaa acaggtacag
    21361 ggcttaatat tcatgatttt agagtaattc atcaaggaaa aacgctgcaa tttgatgtag
    21421 tggtccctga gaaattccag tttgttgata cagaactaga aagaatctta cgtagtcaaa
    21481 tagaagaaaa aatcggagat taccaattgg aaattacgtt tgatcacaat tatcttttat
    21541 attaaatgag aaactataaa aagactcctt aaagttttta atttaaagag tctttttttc
    21601 ttttaattgt ttatggagag cctgacgtgc aagattatct gctcctttat ttttgttttc
    21661 gggcaaccat tgcaggatca ataaagaaaa ttcggataga agttcttgga ttctttgaag
    21721 gtagggaagg aagagctcgt tcttcgttgc gtttttgtcg accgtagaaa ctagtgtttt
    21781 gctgtctgaa aaacagaaaa tcgtttcatt ctgtaagtcg tttttcttta gaaattctaa
    21841 agtttttaaa aagacttcga actctgcttg atggtttgtc cctatagaaa gcgggataga
    21901 tagctgttct tgtttacctt gatagacaat caagattcca cctccactag gaccagggtt
    21961 gccttttgta gaggcatcaa tgtatacttt taacataaaa agagattctc ctttttgttc
    22021 aaactaatgt tatggtaaga ttatatcatt actgaatcgg tgagaggtta gagaatgaaa
    22081 aaaaatattc gatggcagcc tgaactggca catacgatca tctattggtc attgaccttg
    22141 atcattctgt tttttagtct gattctttct ttagaaaaca caggtcctta ttggaaaagc
    22201 aattttgttt taggtatttt tcttgttttt ctatttctag gtttgcggcg ttcttttatt
    22261 ttaaaatcaa actctgtgat tatacgttat gctgcttttt ggaaagaccg agaaatatct
    22321 gtcggacaga ttcgatcttt gacgccaact aatactgggg tgcaaatgat gttgcaagat
    22381 gagaaagccc catctgtttt catgatgcga aaaaaagatc aagtagtcct ttttgatcat
    22441 tttgaaaaaa acggacccaa atcagaatca attccttatc cttatacgaa aaaaagaaaa
    22501 agctaatttt aaaaatcaga acctaaaaaa acgaattttt acaggaaaag aaaagagtga
    22561 gacgtacggt tcatcccgtt tagcgtctca ctctatttgt ttatctattc atgcatacta
    22621 gtatcggtag tctgaacgtt gcctttcttg acatgggcag cttgtgttcc tcgattccct
    22681 tcgatgatct caaattcgac cacttgattt tcttccaaag ttttgaatcc ctcttcttca
    22741 atcgcagtaa aatgtacaaa aatttcttct gtgtcatcat aactgataaa gccgtatcct
    22801 tttttgttgt caaaccattt tacaattcct gtggtcaaag cactcacgac ctttctaaag
    22861 tctgatcata gccttcatta taaagagtat ttataagtac gtcaaataaa ttaaaaacct
    22921 gtaattttat gttttttttc tgaataaaaa aaggtataat tggcatatct taacaaagaa
    22981 gatattttgt tgaataaagt tcgtgaaaag gggtaatcaa atgaagaatg atctgcaaat
    23041 ttcacaagaa acagagctag aaccgattgt tgaagtggct agtaaattag atcttaagga
    23101 agacgatttg gaactgtatg ggaaatataa ggcgaaaatc aatttttctg gaatcaatcg
    23161 atcaaaggat aacgctgagg ggcatttgat cttagttact tccatcaatc caacaccagc
    23221 tggcgaagga aagtcaacga tcactgtagg gttaggagac gcgctcaata aaatcaataa
    23281 aaaatctgtt attgcattgc gagaaccctc acttggtcct gtgatgggaa tcaaaggtgg
    23341 tgctgcaggc ggaggatatg cacaggtatt gcctatggaa gatatcaact tgcattttac
    23401 tggtgacatg catgctatca caacagctaa taatgcatta tctgctcttt tagataatca
    23461 tattcatcaa ggaaatgaat tgaacatcga tgcccgccga gtgatttgga aacgggtagt
    23521 cgatctcaat gatcgtgaac tgcgtaaagt aattgttggc ttgggtgggc caatccaagg
    23581 tgttccaaga gaagatgggt ttgatatcac agttgccagc gaaatcatgg ctatcttgtg
    23641 tcttgcggca gaccttgaag atttaaaggc tcgtcttgca aggatcgttg tcggctatac
    23701 atttgaccgt cagcctgtga ctgctggaga cttgaaagca gaaggcgctc tggcattact
    23761 gctgaaagat gcaatcaagc cgaatcttgt acaaacaatt tacggaacac cagcttttgt
    23821 ccacggggga ccgtttgcca atatcgctca tggctgcaat agtattttag ctaccaagac
    23881 agcattgaga ttagcagatt acgttgtcac agaggcagga tttggtgcag atcttggcgg
    23941 agagaaattt ttagatatca aagtacctaa cttgaaaaaa gcgccggatg ctgtggtgat
    24001 tgtcgccact gtccgtgcat tgaaaatgca tggcggaatg aaaaaagatg aacttaaaaa
    24061 tgagaatctt gatgctttga aagtaggttt tgcaaattta aagcgccata tacgtaatat
    24121 ggaacaatat cagctacctg tgatcgtcgc aatcaatgaa ttcgtaacag atacagacaa
    24181 tgaactcaca ctgttggagc acttgtgtga aaatcagggg attcttgcca agagagcgag
    24241 tgtatgggca gatggagcag aaggcggcgt tgatttagca aaagctgtag tacgattgat
    24301 tgatagaaaa gaagctaatt acaagccatt ataccgccta gaagaaacga tacaagaaaa
    24361 gacagaaaca atcgtcaaga aaatttacgg aggaaatggt gttgttttca gcaaaaaagc
    24421 gcaaaaacaa atagaagagt ttacgaagaa tggatgggac aaactgccga tttgtatggc
    24481 aaaaacacag tattcatttt ctgatgatcc ttcactgcta ggtgcaccgg aagatttcac
    24541 catcacgatc cgcgaaatca tcccaaaatt aggggcagga tttttagtag ccttgactgg
    24601 agatgttatg acgatgcctg gtttgcctaa aaaacctgct gcattgaata tggacgtgac
    24661 aaatgacgga gaagtgttgg gattgtttta agcttgatta taatctacca tcgaaggagc
    24721 tgtattttga ctacagcttc tttttttaga cgatagggaa ttaattttgt ttacactagg
    24781 atcattcttt ttctttattg tattttgagc tatactaaag cataatagga gggtatgatg
    24841 aaaacgacat ttataagaaa aaccggtttt tacggtatgg gtagctcgat tcagctaaga
    24901 atcaacaatg aaaaacgcag tgttctcagt catgaacaaa agataacgct tgatctggaa
    24961 gtgcccttta cgatgcaggt caccttttat tggctaaaaa gccccgttta tacagtgaaa
    25021 gaagcaaagc ctgcttatgt gatcacaatg aattacttga ttcttcaaat ctatccgttt
    25081 ttatttctag caactgggtt gggggccgtt ttcctacaaa atttattttt cagttttttt
    25141 gcagttattg tgttgattgc gttctttttc tatataaaaa accgggcata tttgatcaag
    25201 gaggaaaatt atggcaaact ctgatctttt tttaatcagt ttcaatcgaa tcgaaaaatg
    25261 gatgagagac gagatgggaa atgcacgaaa tatgggattt accgaacttg tcagacgcct
    25321 agctcaaaga aaacaattaa tgatcagaaa atacgaagat gatcttttac agcttgctca
    25381 gttaaggaat gcgatcgtcc atgatcggat cgcagtcgat tttgtcattg cagagccaaa
    25441 tgattggaca gtcaagcgaa tccagctgat cgaacaagaa ctgattcagc ctgaaaccgt
    25501 gctgcctcga tttgcaaaac gtgtaacagg ttttgaacaa gatcttccgt tacttgaact
    25561 gttaaagata gtagcagaaa aaaggtattc acaatttccg ttatacaata aaggaaaatt
    25621 tgtagcattg attactttac gtaatatcgg gttttggcta gcaaaagaaa gccaaaaagg
    25681 tccggttgat ttaacaaata aacgagcgtt ggatttgatc atccaaaacg gaaagtacac
    25741 aaattatcat tttgttccag ctaaaacaca tatttacgag gttgaagcga tgtttcgtga
    25801 gcaagggact ttggaagccg tacttatcac taaagatggg aatcccgatg gtaatctgct
    25861 cgggatcgta cgacctcgtg atatttataa acaaatagaa aaggattgat tttttgttaa
    25921 ttatttattg gatattcagc gctttgctag taggagtgga tcagttggtc aaatggtgga
    25981 tcactgataa tctagcgctt ggagagacga aaaatcttat accaggtatt ttgtcactga
    26041 atcatattcg aaacactgga gccgcatgga gtatgctaga agggaagatg tggttcttta
    26101 ctatagtgac actcattgca gtcgttgtga ttctcacatt gatgattaaa aatcgtgaaa
    26161 aaggtaatcg ctggttcatg attggattga gtttgattct agctggtgcg attggcaatt
    26221 ttatcgaccg tattcgtctt ggctatgtag tagatatgtt ccagacagat tttatgagct
    26281 ttccgatttt taacgtagca gatgtgacac ttgtatgtgg agtgatttgt atgttaattt
    26341 atattatttt agatgaaaag gatcaaagaa aaaaatgaca gaacttaaaa cgacgattca
    26401 ccatgaatct ggtaggatcg ataaagtatt gactaattta ttcaccgatt acagtcgttc
    26461 gcaaattcag ctgtggctga aaaatggagc ggtttctgtc aatggacagc cagtcaaagc
    26521 aaattacaaa gtaaaacaag gagacgagat tactgtcttg atccccgaac cagaaacatt
    26581 agatgtggtc ccagaaaata tcccgttgac gatcgtttac gaagatgaag atgtagcggt
    26641 gataaacaaa cctcaaggaa tggtcgttca tccttcagca ggtcatacgc atggcacaat
    26701 ggtcaatgcg ctgatgtacc atatcaagga tctttctacg atcaacggcg ttattcgtcc
    26761 aggtattgtt caccgaatcg acaaagatac ttccggactg ctaatggttg ccaaaaatga
    26821 ccaggcacac gaatcattag ctcagcaatt aaaagataag acctctttgc gcaaatatgt
    26881 tgctttagtc catggagaga ttcctcacga aaaaggaacc atcaatgcgc caatcggccg
    26941 ttcgaaggaa aaccggaaaa tgcaggcgat aagagaagat gggaagtcgg ctgtcacaca
    27001 ttttactgta ttggaacgtt tcgaaggatt tactctggtt gaattaaaac tagaaaccgg
    27061 aagaacacac cagatccgtg tgcatatgaa gtatatcggt tatccgttag ctggagatcc
    27121 agtatacgga ccgaagaaaa ctttgaaagg caatggtcag tttctccatg cgcaaatgtt
    27181 aggttttacg cacccgacta caaacgaaaa gatgatcttt gaagcacctc ttccagaagt
    27241 ttttgaaaaa actttagaaa aattaagaaa aaacattgca ttttaattta gataacggta
    27301 tagtaattaa caacgaaagc ataaaactta atagaattcc tttaagttta gtcccgtgag
    27361 gctaagaagg gtgggagcaa ggatacgtat ggtgcgccat aagtagattg ctcattgaca
    27421 tagtatagct atcagtgtat cagtatacgc tcagtctaaa cattcctcct gtcctcttag
    27481 ggcaggagtt tttttatgca aaaaagtagg gggaatgaaa atgcaagcaa aagaagtagt
    27541 agatcaggta accatgaagc gagcattgac tcgaattact tatgagatta tcgagagaaa
    27601 ccatagtatc caagatatcg tattggtagg tatcaaaaca cgaggaatct acattgcatc
    27661 aagaattgcc gaacgcctga agcagctaga agatatcgat attcctgtag gagaactgga
    27721 tatcacttta taccgagatg acaaaaaaga aaatccagaa gaaccggaac ttcattcttc
    27781 tgacatccca gtttccttag aaggaaaaga agttattttg attgatgatg ttttgtatac
    27841 aggacgaacg attcgagcag caatggacgc agtaatggat tttggcagac caagaaaaat
    27901 ttcattagca gtcctggtcg atcgtgggca tcgagaattg ccaatccgtg cagattatgt
    27961 tgggaagaac attccaacat ctcgggcgga agagatttta gtagagatgc aagagcttga
    28021 tggacaagat cgcatcatga ttttgaaaga ggaagattag aggagagaga aacatgtcgg
    28081 agaaaaaagg atttcataat gatgacgtgg tgttagatat ccacgatcgc ccaaccccag
    28141 gacattgggt aggattaagt ttacaacact tgttcacgat gttcggagca accgtgttag
    28201 tccccatctt agtaggtatc gatccaggta ttgcgctagt cagttcagga ctaggtacat
    28261 tggtgtactt atttactact aaaggaaaga tacctgcgta cttaggaagc agcttcgctt
    28321 tcattgcagc gatgcagctt ttgatgaaaa cagatggtta tccagcgatt gcacaaggag
    28381 cagtcacaac tggattagtc tatttgattg ttgcgctgat catcaaaaag atcggttcag
    28441 catggctgga taaaatcttg cctccaatcg tcgtcggtcc agtcatcatg gttatcgggt
    28501 tgggacttgc ggcaaatgca gcaacaaatg ctatgtataa tcagggcacg tatgatttca
    28561 agtatattgc tgtagcgctg atcacattag gattaacaat tttcttcaac atgtggctaa
    28621 gaggttttct cggattgatt ccgattttac taggtattat tagcggatac atcgtagcct
    28681 tgctgtttgg aatcgtggat actaagccaa tcgtagacgc tgcctggttt gctgttccta
    28741 actttgagat tccctttgta caatatgaac caaagctata tcttggagca atcacaacaa
    28801 tggcacccat cgcttttgtg acgatgacag agcatatcgg ccatcttatg gtattgaata
    28861 aactaactaa acgtaatttc tttgaagaac cgggtcttca taagacgttg acaggtgatg
    28921 gattagcaca aatcgtggca gcctttgtcg gaggtcctcc tgtaacaagt tacggtgaaa
    28981 acattggtgt tctggctatc acacgtgtcc atagcgtgtt tgtgatcggt ggagctgcag
    29041 tatttgcagt cattttagga ttcgtaggaa aattaagcgc tttgatttta agtatccctg
    29101 gaccagtcat ctcaggaatc agttttatct tatttggagt aattgccgca agtggaatga
    29161 agattttaat cgagaacaaa atcgattttg ataaaaagaa aaatctgttg attgcttcag
    29221 ttatcttagt agtaggaatt ggcggactcg tctttgaagt aggcacattt acattgtcgg
    29281 caatggcatt agcaactgta cttggcatca ttttaaatct tatcttacca gaaacttcac
    29341 gaagcgaaga aaactaagga ggaaacatta tgatcatcaa atcagaacgt atcagcttaa
    29401 agcatttatt gaccgttgag gcacttactg atcaagaagt tatgggcttg atccgcagag
    29461 ggcaggaatt caagcaaggt gctaattgga ctccgcaaaa gcgccagtac tttgctacaa
    29521 acctcttttt cgaaaatagt actcgcacac ataaaagctt cgatgtagcg gagaaaaaac
    29581 tagggctaga agtgattgaa tttgaagcaa gcacaagctc tgtccaaaaa ggggaaacac
    29641 tatacgatac agtactgacg atgtcagcat taggcgtaga cgtagcggtt atccgtcatg
    29701 gtgatgagaa ctattatgac gaactgatac aaagtaaaac aatccagtgt tcgattatca
    29761 atggaggaga cggaagcgga cagcatccaa cccaatgctt gcttgatttg atgaccattt
    29821 atgaagaatt tggtcatttt gaaggtctga aagttgccat cgtaggtgat atcactcact
    29881 ctcgcgtagc taaatccaat atgcagatgt taaaacgtct aggcgcacaa gtattcttct
    29941 caggtcctag agaatggtac gacgatgaat tcgaagtgta cggacattat atgccattgg
    30001 atgaactact agatcaagtt gatgtcatga tgatgcttcg ggtgcaacac gaacgccatg
    30061 atggaaaaga aagtttctca aaagaaggct accatcagga acatgggtta acagttgagc
    30121 gtgcgaagaa gttgcaaaaa catgcaatca ttatgcaccc agcaccagtt aaccgtgatg
    30181 tagaattagc tgattcatta gtggaaggat tacagtcgcg gatcgttcag caaatgagca
    30241 atggagtatt cgtcagaatg gcaatcttag aagcagtgct aaatggaaaa gcgtaattat
    30301 aggaggatga aaaaatgaaa acactcatta aaaacggtca gatcaataca cgaaaaaata
    30361 tgacgacacc agcagaaatt tggatagaag atgggcgaat caaagcaatc ggtacaggat
    30421 tctcagaagc cgaatttgat gaagtgtttg atgcaaaggg acaattgatc acacctggtt
    30481 tagtggacgt ccatgttcat ttgagagagc caggatttac atacaaggag acgatcgaag
    30541 ctgggactcg ctcagctgct cgtggcggtt tcactactgt atgcgcgatg ccgaatttaa
    30601 atcctgtacc tgatacggca gaaaagctga gacaagtata tgacatcatt agaaaagatg
    30661 cagttgtgaa ggtattgcag tatgcgccaa tcacagaaaa tctgcgaagt gagaaattag
    30721 ttgatcaaga agcactgatt gaagaaggtg cttttgcttt cacaaatgac ggtgtaggtg
    30781 tccaaacagc tgggacgatg tatctggcta tgaaagaagc tgcaaaaaac aataaagccc
    30841 tagtagccca tacagaagat gaatctttat tatttggcgg cgttatgcac gctggcaaga
    30901 aggcagaaga acttggtctt cccgggatct taagcgtaac cgagtcttct caaattgcta
    30961 gagatctgtt gctagctgaa gcaactggtg ttcattacca tgtttgccat gtttctacta
    31021 aagaaagtgt ccgtgtaatc agagacgcaa aaaaagcagg aatccacgta acagcagaag
    31081 tttcacccca tcatcttatt ttgatcgacg aagatattcc cgaagatttc ggcttttgga
    31141 aaatgaaccc gccgctaaga ggacgggagg acagagaagc tttgatcgaa ggcttgcttg
    31201 acggtacgat cgattgcatc gctacagacc atgcccccca tggattagaa gaaaaaagcc
    31261 aaagtttcat gaaatcacca tttggtatcg taggaagcga aacagcgttt caactgatct
    31321 atactcattt tgtagaaact ggtcgattca cgttggaaca agtgatcaat tggttggcag
    31381 ttaaaccggc tgagattttt ggtttgaatg caggaacgct aacggttggt gcccccgctg
    31441 atgtagcagt ttttgatatt acacaaactt gcacgatcga taaagaagac tttttatcaa
    31501 aaggggagaa tacacctttt atcggctgga aagtaaaagg cgaaacacaa atgacttttg
    31561 tcaacggaaa acttgtgtgg cagaagggag aataaaagta tggaacgttt gcttatatta
    31621 gaagatggaa cagttttcga aggaaaggct tttggtgcag atgctagtgt cttcggagaa
    31681 atcgttttta caacaagtat gactggttat caagaaacta tcactgatca aagttttaac
    31741 ggtcaaatca ttacatttac ctatcctatg gtgggtaatt atggggtaaa tagagatgat
    31801 tatgaatcga tttctccaac atgcaaagga gtagtagtaa aagaacatgc acgtgtagct
    31861 tcaaactggc gaaaccaaat gacgctagat tcatttttga aacaaaaagg aattcctgga
    31921 atatcaggta tcgatacgcg cgcccttact cgcaaaatcc gtgaagtcgg aacgatgaaa
    31981 gctagtatag ttgatgctgg agattccttc gaacatgcat ttgatcagct gaaagcaacc
    32041 gttctagcaa ccaatcaagt gcagcaagtc tcaactaaca aaccttatcc aagtccagga
    32101 acaggtcgga aagtcattgt cattgacttc ggcttgaaac atagtatttt acgagaattg
    32161 agtaaaagaa actgtaacct cacagtactt ccctataaca cagatgccga gacgatttta
    32221 gcgctatcac cagacggagt catgctgacg aatggacctg gtgatccaaa agatgtgcca
    32281 gaagcaatcg aaatgatcaa aaaaatccaa ggaaaagtac cgatttttgg tatttgtctc
    32341 ggtcaccagt tatttgcttt agccaacggt gcagatacct ataaaatgaa attcggacat
    32401 cgaggattaa atcacccggt acgagaagta gcgaccggaa gaattgattt tacttctcaa
    32461 aatcatggct atgcagtcga tgaagccacg attgatccag aaaaactcat cgtaactcat
    32521 gtagaagtaa atgacggaac agtagaagga gtcaaacaca gagattatcc cgctttcagt
    32581 gtgcagtttc atccagatgc tgcccctggt ccgcatgatg cactacattt atttgatgaa
    32641 tttatggaaa tgatggacgc aggaaaggag cagtaaacag atgccgaaac gtacagatat
    32701 caagaaaatt atggtgattg gatctggtcc gatcatcatt ggtcaagccg cagaatttga
    32761 ctacgcagga acacaagcct gccttgcctt aaaagaagaa ggatatgaag tggttttagt
    32821 aaattctaat cctgcgacga tcatgacgga taaagaagtg gcagatcaag tatatatcga
    32881 accgatcaca cttgaatttg tatcaaggat tcttcgaaaa gagcgtccag atgctctcct
    32941 tccaacttta ggcggacaga caggcctcaa tatggcaatg gagctggcag catcaggtat
    33001 tttagaagaa ttgaacatag aacttctagg aacaaagctt tctgcgatcg atcaagcaga
    33061 agatcgtgat ctattcaaac agttgatgaa agaattgaat cagccaatcc ccgagtctga
    33121 aatcgtcaat acagtagaag aagctgtaga ttttgcaaat aaaatcggct atcctgtcat
    33181 cgttcgtcca gcctttactt taggcggaac tggtggaggt atgtgtgaaa atgaagaaga
    33241 attacgtatc gtatcagaaa atggattaag tctttccccc gctacccaat gtttgattga
    33301 acgaagtatt gcaggtttta aagaaattga atatgaagtg atgagagatt cggctgataa
    33361 tgcaatcgtt gtctgcaata tggaaaattt tgacccggtg ggtatccata caggtgattc
    33421 tatcgtattt gcccctagtc aaacattgtc tgaccatgaa tatcaaatgc ttcgtgatgc
    33481 ttctctttcg attattcgag cacttaagat cgagggaggc tgtaatgtcc aactcgctct
    33541 tgatccacat agttttaatt attatgtgat tgaagtcaat ccgcgtgttt ctcgttcatc
    33601 tgcgttagca agtaaggcga ctggctatcc gatcgctaag ctagctgcta aaatagcgct
    33661 tggattaaca ttagacgaaa tgaaaaatcc tgttacagga acgacttttg cagaatttga
    33721 acctgcatta gattatgttg tagctaaaat tcctcgctgg ccgtttgata agtttgaaaa
    33781 cggagaacgg cttttaggca cgcagatgaa agcaactggt gaagtgatgg ctatcggtcg
    33841 gaatatcgaa gagtctttat tgaaagctgt gcgttcttta gaaatcggta cacaccatat
    33901 cgaacttcct gagttggcag acatcacgga cgatgaccta atccaaaaag tggttcgagc
    33961 acaagatgat cgcctgtttt atgtagctga agctattaga agagggtaca ccatagaaga
    34021 attagcggat ttgacaaaaa tcgatttatt ctttttagat aaactgttgc atatcgtaga
    34081 aatcgaacag cagttgaaac agcagccgtt ggatgacaag atattgaaaa cagcgaaaca
    34141 aaacggtttt acagacaaaa aagtggcgga gctgtgggaa ataaacgaag aagccgttcg
    34201 ccagatgaga aaagaaaatg atttgattcc tgtatataag atggttgaca cttgtgcagc
    34261 ggaattcgaa tcaaaaacac cttattttta tagttcctat gaatatgaaa atgaaagttc
    34321 acgatcagaa aagccctcaa ttctagtact gggatctggt cctatccgta tcggacaagg
    34381 ggttgaattt gactatgcga ctgtgcattc tgtcaaagca atccagcagg caggatacga
    34441 agcaatcatc atgaacagca atcctgaaac agtttcgact gacttttcta tttcggataa
    34501 attatatttt gagccattga ccttcgaaga tgtgatgaat gtgatcgagc tagaacagcc
    34561 gataggagta atcgttcaat ttggtggtca gacagcaatc aacttagccg aacctttgac
    34621 tgctgctggt gtaaaaatct tagggacaac gatcgaagac ttggatcgtg cggaaaatcg
    34681 tgatttattc gagcaagcgc tgaaagagtt ggatattcct caacctttag gggatactgc
    34741 tacaagtaaa gaagaagccg tggttatcgc ttcgagaatt ggttatccag ttcttgttcg
    34801 tccaagctat gtattaggcg gacgtgcgat ggaaatcgtt gaaaaccagc gagatttaga
    34861 agattacatg gaacatgctg tgaaagcatc tccagaacat ccagtactag tggaccgtta
    34921 cttgcttggt ggtgaatgcg aagtggatgc gatttgtgac ggtgaaacag tactcatccc
    34981 agggatcatg gagcatattg agcgtgctgg tgtccattca ggggattcaa tggctgtgta
    35041 tccacctcaa acattatctg aatcgatcaa gaaaacgatc gaagattaca cgatccgctt
    35101 agcattaggt ttgaattgtg tcgggatgat gaatatccag tttgtcatcc atgaaaatga
    35161 ggtttatgtg atcgaagtca atcctcgagc aagtcggact gttccattct taagtaaggt
    35221 gacaggaatt ccaatggcac aaattgcgac gaaagctatt ttgggagaga aactaaagac
    35281 acttggttat gaaaacggac tttatccaga aacaaaagaa gttcatgtta aagcacctgt
    35341 cttttctttt acgaaattgc agaaagttga tacttactta gggcctgaaa tgaaatcgac
    35401 tggagaagta atgggatctg acagacaatt agacaaagct ttgtacaagg cattcgaagc
    35461 ttctggatta cacttgcctg aatatggagc cgtattattt acgattgcgg acgaaacaaa
    35521 agaagaagca ctagaattag ctaaacgttt tacagaaatt ggctatagtc tgataggaac
    35581 gaaacataca gctgcttatt tcgaaaaaaa tggtctggtc gttgaaacag tagcgaaaat
    35641 ttctgaggaa gcagcagaaa aaaatgtagt cgacttaata agagatggta aaacacaagt
    35701 ggttgtcaat acgattgata aagatcgggg aaatgcttct aaagacggtt tcatcatccg
    35761 aagagaagca gttgaacatg gtattccatt gtttacttct ttagacacag cagatgcgat
    35821 cgttcgagtg atggaatccc gtgctttttc aactaaccct atttaacggc gagcacatgt
    35881 taagacaaaa agacgaaaaa ccgttgcagg aggggacaga tgaaacaaga aatgatggaa
    35941 atcgttcagc aaaaacagct ggcaccaaag atttttgaaa tgacattgaa aggtgatcta
    36001 gtaaaagaaa tgcagcaacc aggacagttc ttgcatattc tcgtgccgcg tgcagacttg
    36061 ctattacgcc gaccgattag cttgaatcat atcgaccacg aaacaaatac ttgccgtatc
    36121 atttatcggg tggaaggtga ggggactaga gtcttctcta ctctttctgc cggagaccat
    36181 ttagatgtca tgggtccttt aggcaatggt tttgatttgt ccaatcttca tacaggagac
    36241 gaagtattta taatcggtgg aggaatcggt gtccctcctc tatatgagtt atcaaaacag
    36301 ttagtcgaaa aaggaattaa gccaacgcat tttttaggtt ttgccacaca tgaagtcgtt
    36361 tattatgaaa aagaatttaa tcgtctagga gaaacaagaa tcgctactga cgatggaaca
    36421 tacggtatgc acggaaatgt cggtaattta ttgttaggaa cgaagacgga acctacagct
    36481 gtttttgcat gtggttcaaa cgggttattg aagacagtgg aacagctgtt ttcttcgcat
    36541 caaaatgtcc agttatcatt agaatcccgc atggcatgtg ggataggggc ttgctatgct
    36601 tgcgtttgtc atacgccgga agatgaaaca ggaacaaaaa gtgtgaaagt ttgtgacgaa
    36661 ggaccgattt ttaaaatagg agaggtaatt atatgaatga gaatccttta gcggtcaaac
    36721 ttcctggcct agaattaaaa aatcctatca tgccggcaag cggctgtttc ggttttggca
    36781 aagaatacgg aaaatactat gatttgaatc agcttggatc gatcatggtc aaggcaacga
    36841 ctccaaatgc ccgctttggc aatcccacgc caagagtggc ggaaacacca tctggcatgt
    36901 tgaatgcaat tggtttgcaa aatcctggtc tagacgttgt tatgcataaa cttcttccag
    36961 aattagagga gtacaatgag ttacccatca tcgcaaatgt tgctggtgct tgtgaagaag
    37021 actatgtaga agtttgtagc aaaattggcg aagcaccaaa tgtgaaagca atcgagctga
    37081 acatttcttg cccaaatgtc aaacatggcg gaattgcttt tggaacagat tctgatgttg
    37141 cttttcaatt aacacaagca gtaaagaaag tttcttctgt tcctgtctat gtcaaattgt
    37201 ccccaaatgt aacagacatc gttccaatcg ctcaggcaat cgaagctgga ggggcagatg
    37261 ggtttacgat gatcaatact cttcttggta tgcggattga ccttaagaca agaaaaccga
    37321 ttttagccaa tcaaacaggt ggactctccg gaccagcaat caagcctgtc gctattcgtt
    37381 tgattcacca agtagcaagt atatccagcc ttccaatcat tgggatgggc ggcgtgcaga
    37441 cagtggatga tgttcttgag atgtttatgg ctggagcaag tgctgtagct atagggaccg
    37501 cgaatttcac cgatccgtat atctgtccta aactaattaa agagctccct gtgcgaatga
    37561 gcgaactagg catcgaatcg ctggaaagat taatcaaaga agtgagagag gaacgtgaac
    37621 gatgaatcaa ctagaccaac gaccaattat tgcattagat ttttcaacta ggcaagaggt
    37681 agaagacttt cttcgatttt tcccgaaaga agaaaaattg tttgtgaaaa tcggaatgga
    37741 attgttctac caagaaggac ctgaaatcgt acgttatcta aaagaggctg gtcataatgt
    37801 atttttagat ctgaaacttc acgatattcc gaatacggtc gaaaaagcaa tgcggggatt
    37861 ggcaaagctt ggtgttgatc tcacctgtgt tcatgccgct ggcggtattc gtatgatgga
    37921 ggcggcaatg cgaggattag aagaggggac tccagaaggt ggaaaacgtc cgctacttct
    37981 agctatcact cagcttactt ctacaagtga agaagaaatg catgccgatc aactaatcga
    38041 agtttcttta gaaaaaagtg tgatccatta tgctagctgt gccaaaaaag caggattaga
    38101 tggtgtggtt tcttctgctt gggaagtaga agcgatcaaa gaaatggcag gagacgaatt
    38161 tgtttgctta actccgggta taagaccaga aggaacagtc gcaggagatc agacaagagt
    38221 agtcacacct tctcaggcaa aaaagatcgg ctcaactttt atcgttatag gtcgaccaat
    38281 cactcagtca actaatcctt ataaagcata tcaaacgatt caaacagaat ggagctaacc
    38341 taaaatgaat gttgaacaaa gtatcgcaaa agacttatta gaaattgaag cagtattttt
    38401 aaatccaagt aatcctttta catgggcaag tggaatcaaa agcccaattt actgtgataa
    38461 ccgaatcacg atgagctatc caaaagtccg aaaagagatc gcaaaaggat tagcaagcaa
    38521 aatcaaagaa gcttaccctg aagtacaagt gattgctgga actgcaacag caggaatccc
    38581 tcatgccgca tgggtagcgg aaatactgga tttgccaatg gtctatattc gaagtaaggc
    38641 gaaagatcat gggaaaggta accaaatcga aggacgtata tttgaaggtc agaagatggt
    38701 tgtgatcgag gatttgattt ctacaggcgg aagtgtcttg gaagcagctg aagcagcaaa
    38761 aagagaaggt gcggatatcc ttggtgttgc ggctatcttt acgtatgagt tgccaaaagg
    38821 aaaagcaaat ttcgaaaaag ccgaaatccc tcttatgacg ttgaccaatt attctgtatt
    38881 gattgaagca gctttagaag atcgttatat cgatgagcaa gaattaactc tgctaaaaga
    38941 atggaaaaaa gatcctgaaa attggcagat ttaagcgcgc ttgatgaaga tagagaaatc
    39001 cgatttgtgt ctccagaatc atcaattgga atagcaaaaa tccaaaaact gattgcgaga
    39061 agcatctttt catcaggtat gaaaagaaac ttctatcgtg atcagttttt gattttttta
    39121 aaattcattt ttctaagact ggttagcaga aagaagagaa taatctcttc tcttttttct
    39181 attatatata gattttctct attttttggt aagggagctg tttcaaaaat ggtaaagagc
    39241 ggaaatgttg cgaatatcag ttatttaatg atatgatatt cggaaaaaga aatctcaaag
    39301 ggaggtaact tttatggggt tgaatgaatt tattttagga ttgaacaatt tagagacgat
    39361 cacaagggca cctggatttt ttaaatatac agagcataca gttgcagcac attcgtatcg
    39421 tgtagcatct attgcgcaag ttctaggaga cattgaagaa gcgaatggac aaccgatcga
    39481 ttggaaactg ctctatgaaa aatcattgaa tcatgactac acagagcgtt ttattggtga
    39541 tatcaaaacg ccggtgaaat acgcaagcaa agagttaaga gggatgctgc aaacagttga
    39601 agaaaagatg acggacgaat tcatcaacca agagattcca gctgagttcc aagaagttta
    39661 tcgccgtcgg ttgttcgaag gaaaagatga aaccgtagaa ggagaactat tagccatttc
    39721 agataaggtt gaccttcttt atgaatcttt tgatgaaatc atcaaaaata acccggaaaa
    39781 agtttataag gagatgttca ttgaggcagt gactacgatt aaaagctttg aacatcggcc
    39841 ttctgttcag tatttcttta aagagatatt tccggaacta ttgaaccagg aattttatgg
    39901 aaaagaggcg tttttagaaa ccgtctcaac gattgtaaag aaagaagaaa aataaaaatg
    39961 atatgagatg cagggggaag tatagttgaa aaagaatatg gatgtcgaat ggggctattc
    40021 tggagacaaa ggtccggaaa attgggcgag tttatgtgat tggtttgcga gaggtgcgga
    40081 atttccattg cagtcgccta ttcatttgac caagggggaa gaaaccaaaa tagaaggaga
    40141 cacgctttcc tttcattatc aaaagcaacg gtttactgac aaggaattca aaaacacgat
    40201 tcatttagta ccttttgacc aactcagtta tgtagagttc aagaaagaac gctattattt
    40261 gacagatatt catttccact tgccttctga acatatcatc aacggaaaac aggaagatat
    40321 tgaatttcat tttgttcata tgaacagcaa aaaagagaat ctagttgtgg gtgtcatgta
    40381 ccagctaatg gtacaggaag gttggctgta taatcaagaa aatggggaaa gttggaactt
    40441 agaaaaacat gagcattggg taaatccgga tgtgttcttt ccggagcaaa aaagccattt
    40501 ccattatatt ggctcattga caactccgcc taccagcggg ccaatccaat ggtttgtaat
    40561 ggatcatgta ggaaaactga accaagagtt tcttctcccc ttcgatggtc agtatgctcg
    40621 accgaacaac cgaccaatcc agcccttgaa tggaagagaa atttattatt tcgaggagtt
    40681 tgagcaatga atattcagca gatgaaatac gtggtcgctg tagcaaacaa tggtagtttc
    40741 agagaagcgg caaagaaaat gtttgtctcc cagccaagtc tttcagctgg tatcaaagaa
    40801 ttagaaaccg agttagggat cacgctgttt actcgaacga atcgaggggc atatttaaca
    40861 gaagaagggc aagacttctt aaatcgtgcc gagaaaatat tggtccagct tgatacatta
    40921 gaaactcact atctaacgag tgaaaagaga gacaattttt cgatcgctgc ccagcattat
    40981 gatttcttag gtcctttgac cgcacagcta ctcgaggaat tcaaagacga gatcaaacaa
    41041 tttcggatca tggagacgac aacagcgaga gtgatcgaag aagtcaaaga gcagcacagt
    41101 gagatcggca ttatttatat gaatgagcat aatcaagctg gtatcagaag atatttacaa
    41161 caaggagagc taagtggccg cagcattgga aaattcaaaa cgcatatttt tttaaggcaa
    41221 ggacatccat tagctgaaca cgcagagatc accaaagaag aattgttatc ttatcctcaa
    41281 gttcgtttta cacaggatgg aaataatttc ccgtattttt atgaagacct tatcgagatc
    41341 cctgatcaag aaacagtgat ttatacgagt gatcgaggta ctttgatgaa tatcgttctt
    41401 gaaactgatg cttacgcttc aggatctggt attgtgattg gagggattcg cgagcacttg
    41461 cggttaatcc ctttggcaga aggcgaacta aatgaatttt atatcattta ttcggcgaaa
    41521 cgtccgctca gtgaaatcgc tcaaagattt atcaaagagc ttactcggtt actggaccag
    41581 cagagctaag aaaagcagat gagaacgctg tttattgaac accgttgatc aaaaaagagg
    41641 ggttgtgaca gaattttgtc acaactcctc tttttgtagc aaacaattta taactaattg
    41701 ctgttgtggg aaagtacgca aatatgattc tagaaacact cattgattac ctgttttgtt
    41761 tcatagctgt aattttttct tcttctggtg tcacgataat cgtcttttgg ttttcataaa
    41821 taacataacc aggcttggcg ccatttggtt ttcggatatg ctttacctgt acaagatcaa
    41881 ccggcacttg ggcagagtat cggtattttg aaaaataggc agctaactcg gcagcttctg
    41941 taattgtttc atctgagggc ttgtcacttt taatgatgac gtgcgaaccg ggtatatttt
    42001 ttgcatgaag ccaatagtcg gtttttttag ctgtcttcat tgtcagctgg tcattttgaa
    42061 gattgttttt accaacaaga atgagcgttc cgtcagaaga aaagtattga tcaggctgag
    42121 atggtttctt acgtttttgt ttttttaagt tcttcttttt caggtatcct tctgaagtca
    42181 attcttcttt aattgcttcg atatccattg ggccggcaat ttccagttga gataagacag
    42241 attcaagata ttggatttca tctttggctt cttggatttg ttcgccaatc agcttaacag
    42301 cgttcttcag cttttggtat cgatggaaat atttctgtgc attttgattt ggtgtcaatg
    42361 ccggatctag tgctattttg attggccgat cttcttcata ataattaggt aaaacaactt
    42421 cttttgcgcc gcgtggtact tgtgtcagaa aagtagtaag tagttcgcca tttctacggt
    42481 aattctcagc gttttcggat tctttgagtg tttgttcacg ttttttcaat ttatttttat
    42541 ttcgttttag ttcattttcg atttttcgaa tcagttctcc gccttgttgt tttgcccgat
    42601 ctttctctgc tttttcgtga taatagctat ctaataattg actgagtgtt ggataagctg
    42661 ttacagcaga tgcctgttcg gacaatacct gataagcaat cggtgtgaaa aaatctttat
    42721 tttccgtttc gtaaaaagta ggaataggct gatggctgat ggctgagaaa aattcgtccc
    42781 atacaaccat cttttcatta ggacgttcca gtaatctttt ggtcagctct tgagcgatat
    42841 cgaaaccgat tccctgaaac tgacgttgga ttgcttttgg atctagttca gtttgggaaa
    42901 gccgctgaaa gattttttct ttctcggagc taaatgggtt caagacctct tgttttggcg
    42961 gagcaacata ttctacacca ggaagcaaag aacgataagt gttttgagag cttccaatgt
    43021 gtttgatcgc atccagtatt tttccagttt ctcgattaac taagacgatc gtactatgac
    43081 gtcccattaa ttcaacgatc aatatgatat tttgtagatc acccaactcg tttcgtttag
    43141 caaagtggaa atggatcact cgatcatttt cgatttgttc gatgtcttct aaaatagcgc
    43201 catccaaata ttttcgaagc atcatcacga agttaggcgg attatcagga ttctgataat
    43261 cgatttgggt aatctgtaca cgagcataac ttgggtgcgc agataataat aatcgatggt
    43321 ttttgccgcg tgaacgaatc accagcacga tttcattttc ataaggctga tggattttcg
    43381 agatccggcc agatagtaac gtttcacgca attcgttgac catcgcgtgg gtaaatacac
    43441 catcaaagga catggttcat tcctcatttc ttttcataag tatattcatt ataacgaaaa
    43501 aaagaagaaa aaccaatttg gccgaatagt aaagcaaaag tctagaaaaa tcttggacta
    43561 attacataaa aaacagtgtt tctatcgttg aaatccaaat ttttaggt
//
LOCUS       DAZUKC010000017        43108 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000017 DAZUKC010000000
VERSION     DAZUKC010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.017"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..608
                     /locus_tag="ACG80T_000838"
     CDS             <1..608
                     /locus_tag="ACG80T_000838"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFS4840509.1"
                     /translation="CIVMNPTLITKKELLKKLDISTGVLANLIRNGMPKEGEMFNLDK
                     IITWRENWSKNILGELEVGRVYTNKEISEKFKCSKQGGMRRSHQTNTLVLFSDQTGSN
                     VYKDKWLNGILQYTGMGLKGDQVLDKNQNKVLANSKSNFVKIHLFETFKPKEHTYLGE
                     VYLAGQIYTVNEKDSSGNSRKVYKFPLALINQEQLIEDKDI"
     gene            589..1035
                     /locus_tag="ACG80T_000839"
     CDS             589..1035
                     /locus_tag="ACG80T_000839"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFS4840510.1"
                     /translation="MKIKIYNQEENQTRHIRNLSNAKLEEEARKVSNYNKKMAAKYVN
                     EEEFCFSKTKIYKRNPFVSEYVKRLAKGICQLCQEKGPFIKDGVPYLHCHHIEYLSQG
                     GKDVIENCIALCPNCHARIHELELPDDKEKLLKIVEKRENSFSKIP"
     gene            1378..1701
                     /gene="relB"
                     /locus_tag="ACG80T_000840"
     CDS             1378..1701
                     /gene="relB"
                     /locus_tag="ACG80T_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFS4840511.1"
                     /translation="MRFFKRGNYRAMITVRVSDAKKEWLSYMAEFYGITLSELLKNYS
                     MEQLEDEYNRQTAEIAHKHWIRSGKTTVFMEESLMNLVGWNDLPFKIHARNSEAALIG
                     KYVYW"
     gene            2021..2248
                     /locus_tag="ACG80T_000841"
     CDS             2021..2248
                     /locus_tag="ACG80T_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFS4840512.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            2264..3430
                     /gene="hemW"
                     /locus_tag="ACG80T_000842"
     CDS             2264..3430
                     /gene="hemW"
                     /locus_tag="ACG80T_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFS4840513.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKYGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            3665..4708
                     /gene="hrcA"
                     /locus_tag="ACG80T_000843"
     CDS             3665..4708
                     /gene="hrcA"
                     /locus_tag="ACG80T_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFS4840514.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            4802..5365
                     /gene="grpE"
                     /locus_tag="ACG80T_000844"
     CDS             4802..5365
                     /gene="grpE"
                     /locus_tag="ACG80T_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFS4840515.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            5416..7245
                     /gene="dnaK"
                     /locus_tag="ACG80T_000845"
     CDS             5416..7245
                     /gene="dnaK"
                     /locus_tag="ACG80T_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFS4840516.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            7396..8562
                     /gene="dnaJ"
                     /locus_tag="ACG80T_000846"
     CDS             7396..8562
                     /gene="dnaJ"
                     /locus_tag="ACG80T_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFS4840517.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(8930..9667)
                     /locus_tag="ACG80T_000847"
     CDS             complement(8930..9667)
                     /locus_tag="ACG80T_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS4840518.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(9669..11171)
                     /locus_tag="ACG80T_000848"
     CDS             complement(9669..11171)
                     /locus_tag="ACG80T_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFS4840519.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            11430..13424
                     /gene="uvrB"
                     /locus_tag="ACG80T_000849"
     CDS             11430..13424
                     /gene="uvrB"
                     /locus_tag="ACG80T_000849"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFS4840520.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            13434..16250
                     /gene="uvrA"
                     /locus_tag="ACG80T_000850"
     CDS             13434..16250
                     /gene="uvrA"
                     /locus_tag="ACG80T_000850"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFS4840521.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            16513..17778
                     /locus_tag="ACG80T_000851"
     CDS             16513..17778
                     /locus_tag="ACG80T_000851"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFS4840522.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            17782..18513
                     /locus_tag="ACG80T_000852"
     CDS             17782..18513
                     /locus_tag="ACG80T_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFS4840523.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            18648..19532
                     /gene="rapZ"
                     /locus_tag="ACG80T_000853"
     CDS             18648..19532
                     /gene="rapZ"
                     /locus_tag="ACG80T_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFS4840524.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            19529..20527
                     /gene="yvcK"
                     /locus_tag="ACG80T_000854"
     CDS             19529..20527
                     /gene="yvcK"
                     /locus_tag="ACG80T_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFS4840525.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            20561..21496
                     /gene="whiA"
                     /locus_tag="ACG80T_000855"
     CDS             20561..21496
                     /gene="whiA"
                     /locus_tag="ACG80T_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFS4840526.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            22064..22720
                     /locus_tag="ACG80T_000856"
     CDS             22064..22720
                     /locus_tag="ACG80T_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840527.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            22899..23464
                     /locus_tag="ACG80T_000857"
                     /pseudo
     CDS             22899..23464
                     /locus_tag="ACG80T_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(24163..24684)
                     /locus_tag="ACG80T_000858"
     CDS             complement(24163..24684)
                     /locus_tag="ACG80T_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFS4840528.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            24882..28562
                     /gene="nifJ"
                     /locus_tag="ACG80T_000859"
     CDS             24882..28562
                     /gene="nifJ"
                     /locus_tag="ACG80T_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFS4840529.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            28751..29638
                     /gene="cdaA"
                     /locus_tag="ACG80T_000860"
     CDS             28751..29638
                     /gene="cdaA"
                     /locus_tag="ACG80T_000860"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFS4840530.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            29635..30804
                     /locus_tag="ACG80T_000861"
     CDS             29635..30804
                     /locus_tag="ACG80T_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFS4840531.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            30828..32183
                     /gene="glmM"
                     /locus_tag="ACG80T_000862"
     CDS             30828..32183
                     /gene="glmM"
                     /locus_tag="ACG80T_000862"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFS4840532.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(32255..34060)
                     /gene="glmS"
                     /locus_tag="ACG80T_000863"
     CDS             complement(32255..34060)
                     /gene="glmS"
                     /locus_tag="ACG80T_000863"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFS4840533.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERYSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            34470..34760
                     /locus_tag="ACG80T_000864"
     CDS             34470..34760
                     /locus_tag="ACG80T_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840534.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            34773..35798
                     /gene="sppA"
                     /locus_tag="ACG80T_000865"
     CDS             34773..35798
                     /gene="sppA"
                     /locus_tag="ACG80T_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFS4840535.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            35801..36460
                     /locus_tag="ACG80T_000866"
     CDS             35801..36460
                     /locus_tag="ACG80T_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFS4840536.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            36617..37549
                     /locus_tag="ACG80T_000867"
     CDS             36617..37549
                     /locus_tag="ACG80T_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840537.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            37703..38992
                     /gene="tig"
                     /locus_tag="ACG80T_000868"
     CDS             37703..38992
                     /gene="tig"
                     /locus_tag="ACG80T_000868"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFS4840538.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            39239..40489
                     /gene="clpX"
                     /locus_tag="ACG80T_000869"
     CDS             39239..40489
                     /gene="clpX"
                     /locus_tag="ACG80T_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFS4840539.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            40572..41159
                     /gene="yihA"
                     /locus_tag="ACG80T_000870"
     CDS             40572..41159
                     /gene="yihA"
                     /locus_tag="ACG80T_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFS4840540.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(41233..41388)
                     /locus_tag="ACG80T_000871"
     CDS             complement(41233..41388)
                     /locus_tag="ACG80T_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFS4840541.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            41524..42852
                     /locus_tag="ACG80T_000872"
     CDS             41524..42852
                     /locus_tag="ACG80T_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840542.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            42863..43072
                     /locus_tag="ACG80T_000873"
     CDS             42863..43072
                     /locus_tag="ACG80T_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840543.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
BASE COUNT        14293 a         7363 c         9311 g        12141 t
ORIGIN      
        1 gctgcattgt tatgaatcca acattgatta ctaagaaaga attactaaaa aaattagata
       61 tttctactgg tgtattagct aatttaatta gaaatggcat gccaaaagag ggagaaatgt
      121 ttaatcttga taagattatt acatggcgag aaaattggtc taaaaacata ttaggtgagt
      181 tagaagttgg tagagtatat actaacaaag aaatttcaga gaagtttaaa tgttccaaac
      241 agggtggaat gagaagatct catcaaacta atactcttgt actgtttagt gatcaaacag
      301 gaagtaatgt ttataaagat aagtggttaa atggtatttt acaatatacc ggtatggggc
      361 taaaagggga tcaagtattg gataagaatc aaaataaagt tttagccaat tcaaaaagta
      421 attttgtaaa gattcattta tttgaaacat ttaaaccaaa agaacacaca tatctgggag
      481 aagtatattt agcaggacaa atatatactg ttaatgaaaa agatagttcg ggcaacagca
      541 gaaaagtata taaatttcca ttagctttaa taaatcaaga gcaattgatt gaagataaag
      601 atatataacc aggaagaaaa tcaaactaga catattcgta atttatctaa tgctaaatta
      661 gaggaagaag caagaaaagt tagtaattat aataaaaaaa tggctgcaaa atatgttaac
      721 gaagaggagt tttgcttctc aaaaacaaaa atatataaac gtaatccctt cgtatctgaa
      781 tatgttaaac gacttgcaaa aggtatttgt caactttgcc aagaaaaagg gccatttata
      841 aaagacggtg taccttattt acattgtcat catatagaat atttgtcgca aggtggaaaa
      901 gatgtgattg aaaactgtat cgcgttatgt cccaattgtc atgctagaat tcatgaactt
      961 gagttacctg acgataaaga aaaattgctg aagatcgttg aaaaaagaga aaatagcttt
     1021 tctaaaatac cgtaatttat ttttatagga caaataagct aatgtgagct atgccttcta
     1081 tataagacta agttctatta taagtaacga agtaattctc gttccttcct gtagtaatat
     1141 tttttaaata acctataggc ttcttcaaaa gtacatcctc cagtaaattc taagtactgg
     1201 aggattcttt ttctagtgtt gctttactca tgaaaaagta gttggctact tcatcaaaat
     1261 ttctgaactt ttttaatgta taataaagag ctcgatcatt tactacaagg atgctagctc
     1321 ctacattttc acagtttttt agcaagtaaa tttttctaaa atcacatcag gaaatgcatg
     1381 agattcttta agagaggcaa ttatagggct atgattacgg ttcgtgtaag tgacgccaaa
     1441 aaagaatggc tttcttatat ggctgaattt tatgggatta ctttatctga acttctaaaa
     1501 aactattcga tggaacaact agaagatgaa tacaatcgtc aaacggctga aattgcccat
     1561 aaacattgga taagaagtgg gaaaacaaca gtattcatgg aagaatcctt aatgaatttg
     1621 gtgggctgga atgacctgcc gtttaaaatt cacgcccgaa attcagaagc agctctgatt
     1681 ggcaaatacg tgtattggta gagatagaag ataatcaatt cgttgttatc gctattcaag
     1741 tagggcaccg aagaaatgtc tatgattaag agactgttta tttttacaaa aaaataataa
     1801 ttggaatcta gttaaaactc ttgaagtgag agatttgact agattttttt atgaataaaa
     1861 taaggacaaa attgtttagg aagaatcatg agtgtaaaaa aatcatccaa gaagtcgatt
     1921 cttggatttt ttagtggatt tgttcgccaa atacgtttag tatgcgtata tgagaggttg
     1981 taagcaaagc gttgtggtct gagcacttaa ggaggaatct gtgaaaatag cttttcatat
     2041 ttttgcagat tttgacttaa tgccgaaggt cagctttgcg attaccgttt atcgagaggc
     2101 tgtaaaagca ccgtgtagct ccgatgacaa taagcaagca tttttagagc taggtttcct
     2161 tgattctaaa tatgcggctt atttcaatga gttggtgctt gaacgcgatt ttaagaaggt
     2221 cgaaaacagg cacgactttt cgacgtaagg agaaagaatt tcaatgatag aaacaataaa
     2281 tcccgcagaa aacaaggttt ctgcgtacat tcatatccca ttttgcgagc atatctgcta
     2341 ttactgtgac tttaataaag tttttttaga agggcagcca gtagatgagt atgtggaaat
     2401 gctgctgaat gaaatgagat taatgctgga aagtcatcct acagcgaatt tggaaacact
     2461 ttatgtagga ggcggaacac caacttcttt atctgctaaa caacttgatc gactattatc
     2521 aggtgctaga gagatcctgc cttttcaaaa tggtaatgag tttactgtag aagcgaatcc
     2581 tggtgatttg acccgtgaaa aacttcaagt gatgaaaaac tatggcgtca atcgtttatc
     2641 catgggagtg cagacatttg acaatcgctt attgaaaaaa attggacgta aacatactgc
     2701 agaagatgtg tatcaaacaa tgcaattttt agaagcagaa aattttacca atgtaagcat
     2761 cgatttgatt tatgcattac cgggacaaac actagaagga tatcgggata ctttggaaaa
     2821 agctttggct ttggatctgc ctcactattc tttgtactca ttgatcctcg aaaacaaaac
     2881 aatgttcatg aactgggtac gacaaggaag actcgaactg cctgatcagg aaacggaaac
     2941 cagaatgttt gaagagacga ttgaagcaat ggaaaagtat ggacgtcacc aatatgaaat
     3001 aagtaacttt gggctctccg gacatgaatc tcaacataac ctcatgtact ggaacaatga
     3061 ccactatttt gggtttggag ctggtgctag cggttatctt ggagatattc gttaccgcaa
     3121 caaaggacca atccaacact atttacgccc attacgagca aatgaacgcc cggtattgga
     3181 agaagaaaaa ttgacacgaa aaaatcaaat agaagaagaa atgttcttag gactaagaaa
     3241 aaaaacaggg attactaaaa aacatttcta tgatcgctat caaaaaacaa ttgaatcggt
     3301 gtacggtgat gtgctaaggg aattagagca agaagggtta cttgttaatg aagaagaccg
     3361 tgtttatttg acaccaagag ggactttttt ggggaacaat gtgtttgagc gctttttatt
     3421 tgaaaaatag tttgaagaat atttgttttt tatcgaaact tgaaaagaaa aagtttagaa
     3481 agtttgacaa tgaggtattg aagtattgat ataactggat ttacatcaat tatttagaat
     3541 tagcactctt gtgtttagag tgctaaaaaa tggagtcttt ctcttgacag gagaagggct
     3601 tcttgctata tttataagtg aattagcact tagaatatat gagtgctaat gagaggtgag
     3661 gaagatgtta acaaagcgac aaaacgacat tttacgtctg atcatccaac actatacgag
     3721 tagtggcgtg cccgttggtt ccaaaacatt aatgtctgag ggagtcaagg ccagctcagc
     3781 aacgattcgc aatgatatga aagcattaga agatgaagga ttgctgttga agactcactc
     3841 ttcttctggg cggattccat ctgctttagg ctatcgttat tacgtggatc atttgttaaa
     3901 acctgctcgc gtagcagaag atgatttgct gctgattcgt cagtctctag gcagagaatt
     3961 tcatgaaatc aatgagatta ttaagcaatc tgctgagata ttatctgagc tgacaagcta
     4021 tacgactttt tcattgggac ctgaaataaa agaccgccga ctgactggct ttcgtatcgt
     4081 acccttgaat aatcgacaga ttattgctat cgtcgtaaca gatatgggga atgtagaaag
     4141 ccaagtgttc actcttcccg aatatatggg aagtcaggaa ctagaaaaaa tggtacagat
     4201 tgtaaacgac cgtctagtcg gagatcctct gatgacggtg taccataaac ttcgtacgga
     4261 gattccgatg attcttcata aatatttcca gacgacagaa gggatgtctc agttatttga
     4321 caccgttctt ggtcatgttt ttgaagaaaa ggtttatgtc agtggacaaa tgaatctctt
     4381 ggattatgag ccccgtcaag atcttgatca gttcaaatcg atgttttctt ttatgaaaga
     4441 ctcagacgga cttacacaaa tgatcactcc gatggatagc aatattcata tcaagattgg
     4501 tgctgaatta ggcaatgatt tattgcagaa tatgagtatg attcaagcaa gttacgagat
     4561 agatggtcat ggaagaggta caatagcctt gttagggcct actagtatgc cttattctcg
     4621 gatgttcggt ctagtcgatg tttatcgaag agaactagca gctaaacttg ctgattacta
     4681 ccgctcgctt gacttgtcaa actcgtaatg tgaataacga agctatttgc agctgatcgt
     4741 tctctctttc ttagtaagag caatcaaaca gctgactgaa taatgaaagg aagactgtat
     4801 cgtgaaagaa aatcaagaag aactagataa agaaatgaca gatgctcagc cagaaccgga
     4861 aatcgatgtc gaagctgcag aagattcggg tatttcagaa gctgaagcgg aagaattcga
     4921 aacagcgaaa ttaaaagctg agttagaaga aatggaagat aaatacttgc gggctcgtgc
     4981 tgaaatcgca aatatggcaa atcgaggaaa aaatgagcgt gagcaattac aaaaataccg
     5041 ttcacaagat ttagccaaaa aattacttcc atcaattgac aacctggaac gtgcattagc
     5101 cacagaagtg tcagatgatc aaggggcagg tttgaaaaaa ggcgtggaaa tggtcttgga
     5161 aagtttgcgt aacgcactgg aagaagaagg aattgaaaaa atccctgcta aaggagaagc
     5221 ctttgatcct aatcttcatc aagcagtaca aactgttcca gcgactgaag atacaccagc
     5281 cgatacgatt gtagaagtac tacaagaagg ctacaaatta catgatcgcg tgttaagacc
     5341 aactatggtc attgttgcac aataattgtg cggatataaa acttacactt tcaattaaag
     5401 aaggagacgt taattatgag taaaattatc ggtattgact taggtacgac taactctgca
     5461 gtagcagtat tggaaggaaa cgaagcaaaa atcatcgcta acccagaagg aaaccgtaca
     5521 acaccttctg ttgtatcatt caaaaatgga gaaatccaag taggggaagt tgcaaaacgt
     5581 caagctgtga caaacccaaa tacgatttca tcaatcaaac gccatatcgg tgaagcaggt
     5641 tacaaagtag atgtagaagg taaatcatat acaccacaag aaatctcagc aatgatcctt
     5701 caatatatca aaggattcgc agaagactat ttaggtgaaa aagtcgaaaa agcagttatc
     5761 actgtgccag cttactttaa tgatgcacaa cgtcaagcaa caaaagatgc tggtaagatc
     5821 gctggtctag aagtagaacg tatcgtcaat gaaccaactg cagctgctct tgcctatggt
     5881 ttagataaaa cagatcgcga cgaaaaaatc ttagtattcg accttggtgg cggtactttt
     5941 gacgtatcta tccttgaact aggagacggt gtctttgatg tattgtcaac agctggcgac
     6001 aaccatctag gtggggacga ctttgataac aaaatcatcg actacatggt agcagaattc
     6061 aaaaaagaaa acagcattga cttgtctcaa gacaaaatgg ctttacaacg cttgaaagac
     6121 gctgctgaaa aagcgaaaaa agatttatct ggcgtatcaa gcacacaaat cagcttgcca
     6181 tttatcacag caggagaatc tggaccattg catttggaaa tgacattgac acgtgcaaaa
     6241 tttgatgaat taacagctga cttagtagaa cgtacaaaag taccagtacg tcaagcgtta
     6301 aaagatgcag gtctgtcaca atcagaaatc gacgaagtta ttttagtcgg tggatctact
     6361 cgtatccctg cagttgtaga agcagtacgt aaagaaacag gaaaagaacc aaacaaatca
     6421 gttaaccctg atgaagtagt tgctatgggt gctgctatcc aaggtggcgt gatcactggt
     6481 gatgtgaaag atgttgtttt acttgacgtt acaccattgt cattaggtat cgaaacaatg
     6541 ggtggagtgt tcactaaatt gattgaccgc aatacaacga ttccaacaag taaatcacaa
     6601 gtattctcaa ctgcggcaga taatcaacca gcagtagaca tccatgtatt acaaggtgaa
     6661 cgcccaatgg ctgctgacaa caaaactcta ggaagattcc aattgacaga tattccagca
     6721 gctcctcgtg gtattccaca aatcgaagta acattcgata ttgataaaaa cgggattgta
     6781 aatgtatctg cgaaagatct aggtacacaa aaagaacaaa aaatcacgat caaatcttct
     6841 tcaggtctaa cagacgaaga aatcgaacgt atggtgaaag atgcagaagc aaacgctgaa
     6901 gcagataaag ctcgtaaaga ggaagttgac ctacgtaatg atgtagacgc attgctattc
     6961 tctgtggaca aaacattgaa agaattagaa ggcaaagtgg acgaagaaga agtgaaaaaa
     7021 gcagaaacag ctcgtgacga attaaaagcc gctgttgaag caaacaacat cgaggaaatg
     7081 aaaactaaac gcgatgcatt gaacgaaatc gttcaaaact tgactgttaa actatacgaa
     7141 caagctgcac aacaacaagc tcaagaaaat ccagaagcag ctcaaggcgg agcagatgat
     7201 gtcgttgatg ctgattttga agaagtcgat ggcgacgata aataatcagt tcactgaatt
     7261 tcgagagaag gccaaagttg ctactttggc tttttctttt gaaaaatagt ttgaagtatc
     7321 ggtttttgtt tcaaacaaaa tatgatatga taacaagcga cgtgaaaatg atgagaacca
     7381 ataggaggaa agcctatggc aacaaaacgt gattattatg aagtcttagg gctctcaaaa
     7441 ggagcatcag aagatgaaat aaagaaagct tatcgtaagc tttcgaaaaa ataccatcct
     7501 gacatcaata aagaacctga tgcggaagaa aaattcaaag aagtatcaga agcatatgag
     7561 attttaagtg acccgcaaaa acgcgctgca tacgaccaat acgggcatgc aggcacagat
     7621 ccaaattacg gtgctggtgg agctggtggc ggatttggtg gattcggcgg cttctctggt
     7681 ggcggcttcg gtggttttga agatatcttc gactctttct ttggaggagg cggtcggtca
     7741 gtcgatccaa atgcgcctcg tcaaggtgct gatttgcaat atacggtcga cttgtctttt
     7801 gaagaagcga tttttggtgt agaaaaagaa atcaagtata atcgggaaga aatctgtcat
     7861 acttgtggtg gaaacggtgc aaaaccaggc acacaaccaa ctacttgtca taaatgtcat
     7921 ggtgcaggta cgatcaatgt agaacgccaa acgccacttg gacgagtgat gagccgtcaa
     7981 acttgtgatg tttgtcatgg cacagggaaa gaaatcaaag aaccttgtcc aacctgtcat
     8041 ggctcaggac atgagaagaa agcacattca gttaaagtga atgtaccagc tggtgtagaa
     8101 gaaggacaac aaatgcgttt agctggtcaa ggagaagctg gagaaaacgg cggaccattt
     8161 ggtgatttgt atgtggtttt tcgtgtagaa gaaagtgata tctttgatcg tgaaggttct
     8221 gaaatttact atgaattgcc tttaagtttt gtacaagcag ctttggggga tgaagtccaa
     8281 gtaccaactg tccatggaga tgtgaaattg aagatccctg ctggaacaca gacaggtacg
     8341 aacttccgtt tacgtggaaa aggcgctccg aagttacgtg gtggtgcaac aggcgaccaa
     8401 catgtcaaag taaaactgat cacaccgaaa aatctaaatg aacaacaaag agaagcatta
     8461 cgtgcattcg ctgaagctgg cggcatcaaa gttgaagaac agcaagaaga cggtttcttc
     8521 gataagttta aagatgcttt tggcgggaaa aagcggaagt agtttttcta gtaaaaatat
     8581 aaagtagact tgcccaaatt ttctatttct agtaaaaacg ctaagtcatt aataattaaa
     8641 aaccagacga acaatcatac gttcgcctgg tttttttgta gttgtcatgt ttgtaccaac
     8701 aaaaaagcca gctttagaat aagataatca tcatagtttc tgaaggtggt atcgactatg
     8761 tacttgatat cgtcaatgaa ccgatggtac tttcttatgc gatggaacag gtagagcgat
     8821 tgcttgtttg tgcaggagaa caagcgatgc ggctggtaca gttaagtgaa aaaacaagaa
     8881 actaaaaatg cccggtcgtc atgaagcgac cgggcatttt tggtttttat taaatattta
     8941 atactttatt caagaaatct tttgtccgtt cattttgcgg attttcaaag atttgttgag
     9001 gcgttccgtc ttctaaaaag tttccgtcgt caatgaacat gacacgattg gccacttctt
     9061 tagcgaagcc catttcgtgc gtaacgataa ccatcgtcat accttgttct gctaactttt
     9121 tcatgacgtt caagacatca ccgaccattt cggggtcgag tgcagatgta ggctcatcaa
     9181 aaagcatgat gtcagggttc atcgctagag cacgagcaat agccacacgc tgcttttgtc
     9241 caccggaaag catttcagga tacatatctt ttttatcttc tagaccaact gtctgcagca
     9301 atttgattgc ttttttctct gcttcttctc tagattcttt cttcaggtcc atcggtgcta
     9361 aaatgatgtt ttccaaaata gaaagatgag ggaataggtt aaagtgctgg aataccattc
     9421 cgatatgttg tctcaccaag ttgatatctg tgcttttatc catcaagtgg gcgccgtcaa
     9481 taataatttc accgtcagaa ggttcttcta aacgattcaa gcaacgcaaa aatgtacttt
     9541 ttcctgaacc tgaagggcca atgatacaga cgacatctcc ttcgttgatg gacacattga
     9601 tgtcgtttaa tactgtgtta tcgccgtatt ttttgactaa atgctcaact aagatttttt
     9661 ctgccatctt acttcacctt cttttctaaa acttttgcta attttgtcaa tgctgtgatc
     9721 aaaatcaagt acatgatcgc aatgatcaag taaacatacg tactttgtgt tgtacgtgca
     9781 acaatgatct taccagtttg cagcaattcg actacaccga ttgcggaaat gatcgttgtg
     9841 tcttttaatg agataacgaa ctgattgacg aatgatggga tcatgatgcg tatcgcttgc
     9901 ggcaaaatga ttttttgcat tgtccgatta taagaaagac ctaaacttct ggaagcttcc
     9961 atttgtccga caggaacggc gttgatcccg cctcgaacaa tctctgcgat ataagcactt
    10021 gcgttaagag tcaaggtgat aacaccagca aggaagtcag gaatcgtaaa tccaacgata
    10081 ccaggcaacc caaagaaaat aaagaacgca agaaccatca ttggaattcc tcggatgata
    10141 tcgacataaa tactagcaat tgtgcgaaga gcgcgtattg gtgctacact gaataatcca
    10201 aaaatcacac cgattactat agccaaagca aaggagatca aagttaggag aattgttctc
    10261 caaagtcctt tcaacaatgc ttgatagttg ttttggataa gtccaagaaa tgttgattca
    10321 tcagcagtgg aagactcact ggaagtttct gaaccatccg ctacatattc tttaataatc
    10381 ttatcgtatt cgcctgttct ttttaattct ttcaatcctt cattgaacat attaagaagc
    10441 tcaggatttt gtcctttttt aacggcaaat gcatattttc cgccagattc ttttttgatc
    10501 ggtgtttcca attcttgtcc ttgtttgatc gcataaccga ttaccggata atcatccatc
    10561 attgcatcga ttgcgccaac ttttaacccg tcgtacaatt gatcagcagc atcaaaatat
    10621 ttgatagaat agccatattc agattcatgg tcgcttaaaa attcagcact ttcggtacca
    10681 acttttgctc cgactgtttt tcctttcaaa tcttcataag acttgatcga agtatctcct
    10741 tttttgacag ctaattggat cccgctatca aagtaagaat cagaaaaatc atagcttttc
    10801 tttcgttcat ctgttacagt cattccggca atcattccgt ctgcttgtcc tgattccaaa
    10861 gcttgcaaag cagcagagaa accacggtag tccattcgga tgttaaatcc ttgcagtttg
    10921 gcgatgtttt ccatcaattt tacatcgatg ccttcgtact cgccctttgc gttttgaaat
    10981 tcaaatggag caaaagcaga gtcactggca attacgtata cgtctttttt gggctctatt
    11041 ttttgcattg tctcgcttgt gcttgactct gccgttgtag tgttgtcagc ttgtgcaaca
    11101 tgtccaaacg caagtgtcac aagtcctgtc attaagaaaa caaatgagtg aaataaatgc
    11161 tttcttttca taatcaacct cctaaatttc caatatttta ttattatagg cgaaaagtgc
    11221 tgaatcagac aagactgtaa gctcgaaaaa atgaaaaaaa tgcgagtttc taacatttta
    11281 tgttataaaa cgtaacatgc ataataagat aaaaaagtgg cgaaagtatg ttcggtgtgg
    11341 tataattttt tagtaaggaa tgcctttgct ttccttttct ttttattggt cgtaaaatga
    11401 aaaggaatga atgaaaagga ggacgaagaa tgattgagag agatacctcc cgacactttg
    11461 agcttgtctc gaaataccaa ccggctggtg atcagcctga agcgattaac caattagtgg
    11521 atggcgtagt cggcgggaaa aaagcacaaa tcttattagg tgcgactgga acagggaaaa
    11581 catatacgat ttccaatctt attgagaaag tgaataaacc aacactaatt attgcccata
    11641 acaaaacatt ggctggtcag ctgtacgggg aattcaagga atttttccct aacaatgctg
    11701 ttgaatattt tgtttcttat tatgattatt atcagcctga agcttatgtg ccttcaagcg
    11761 atacgtatat cgaaaaagat tcaagtgtaa atgatgagat cgacaaatta cgacattcgg
    11821 ctactagttc attattagaa agaaatgatg tgatcgtcat tgcatccgtt tcatgtattt
    11881 ttggattagg ttcgcctttt gagtaccaaa aacaagtagt ttcgattcga cagggagcag
    11941 aactagatcg caatcagttg attcgtgact tagtcagtat ccaattcgaa agaaatgata
    12001 tagattttca gcgcggtcgt tttcgagtgc gcggtgatgt cgtggagatt tttccggctt
    12061 caagagatga acgtgcctta cgtgtggagt tttttggaga tgaggtcgaa cgtatccgag
    12121 aagtcaatgc actgacaggt gaagttttag gagaaacgga acacgtggca atcttcccgg
    12181 cgactcactt tgtgacgaat gatgaacata tggagcatgc cgtagcgaat atcaaagcgg
    12241 aattagaaca gcgtttaact gtcttgcgaa atgaaaacaa acttctggaa gctcaacgtt
    12301 tagaacagcg aaccaattac gatattgaaa tgatgttaga aatgggctac acgtcgggaa
    12361 ttgaaaatta ttctcggcat atggacggac gtaaagaagg tgaaccgcct tatacgctat
    12421 tggatttctt tcctgaagat tttttgatcg tggcggacga atcgcacgta accatgcctc
    12481 aagtcagagg aatgtataat ggtgaccgag cgagaaaaca gatgctagta gattacggtt
    12541 tccgtttgcc atctgcttta gacaatcggc cacttcggtt agaagagttt gaaaaacatg
    12601 tcaatcagat catttatgtc tctgcgacgc caggaccata tgaacatgaa caaacagata
    12661 ccgttatcca acaaatcatc cgtccgacag ggctgcttga cccagtgatc gaagttcgtc
    12721 caatcatggg acaaatcgat gatctagtag gcgaaataaa tgaacgagtc gaaaaagacc
    12781 agcgggtgtt tgtaacgaca ttgacgaaaa aaatggcaga agacctcaca gattatttca
    12841 aagaattagg gatcaaagtc aaatatttgc atagtgatat caaaacgctg gaacgaacag
    12901 aaatcattcg agatcttcga ttaggtgaat ttgatgtgtt agttggaatc aatttgcttc
    12961 gtgaaggatt agacgtacct gaagtttccc ttgttgctat cttggatgcg gataaagaag
    13021 gctttttacg tagtgaacgt tccttagtcc aaacgattgg acgagctgct cggaatgaag
    13081 aaggtaaagt aatcatgtac gcagacaaag taacagattc tatgcgtcta gcgatggatg
    13141 aaacatcaag acgccgtacg attcagcaga agtacaatga agaacatggg atcgttccaa
    13201 aaacgatcat taaagaaatc cgagatttga tttctattac gaaagaatcg gaagatgata
    13261 caaaagaagc ggtacaagtc tcgtatgaag aaatgacgaa agaagaaaaa gatacgttat
    13321 tgatgaaatt agaaaaagaa atgaaaaatg cggcgaaagc attggatttt gaaacagccg
    13381 ctaatgtgcg agatatgatt ttagaattaa aagcttcaaa gtaggaggac aacatggcta
    13441 acgataaaat tacgattcac ggtgcacgtg cccataactt gaaaaatata gatgtaacga
    13501 ttccacgaga taaattcgtg gtcgttacag gtttgtccgg ttctggaaaa agttccctag
    13561 catttgatac gctgtacgct gaagggcagc ggagatacgt agagagcctt tctgcttatg
    13621 ctcgtcagtt tttaggacaa atggataagc cggacgtaga tagcatcgac ggattgagtc
    13681 cggcgatttc tattgatcag aaaacaacaa gtaaaaatcc tcggtcgaca gtaggtactg
    13741 tgacggaaat caatgactat ttacgcttgt tgtttgcacg tgtaggacat ccgatttgtc
    13801 ccaacgatca tatcgagatt accagtcaat cagtagatca aatggcagat caagtaatgg
    13861 ctttgcctga gcgcacaaag atccaagtgt tggcaccgat cgtggtaaag aaaaaaggac
    13921 aacataaaaa aatattcgaa cgaatccaaa aagagggata cgtccgcgtt cgagtcgatg
    13981 gtgagatcta tgatttaagt gaagtgcctg agctggagaa aaacaaaaaa catgatatcg
    14041 cgatcgtcat cgaccggatc attgtgaaag aagggattcg ttctcgtcta tttgattctt
    14101 tagaggcggc tctgcgattg gcagacggct atgctctggt tgacgtgatc ggtgaagaag
    14161 aaatgttgtt tagcgaacac tatgcctgtc cttattgcgg atttactgtt ggcgaactag
    14221 aaccaagact gttctcattc aatgcgcctt ttggagcttg tccagactgt gatggtttag
    14281 gtgtcaaatt agaagtcgat caagatttag tgattccaga tagaacgaag acacttagtc
    14341 aaggtgctat cgttccatgg aatccaatca gctctcagta ttatcctcaa atgttagcgc
    14401 aggcttgtga aagtttcggt atcgatttag atacaccatt cgaagaatta ccggaagagc
    14461 atcaggatat catcttgaat gggacagatg atcttttcca tttccattat gaaaatgatt
    14521 ttggtggcgt tcgcgatgta gaaacgactt tcgaaggggt attgaagaat atcgagcgtc
    14581 gttatcatga tacgaacagt gatttcacgc gtgagcaaat gcgtctttat atgacagaat
    14641 taacttgtca gacatgtaaa ggttatcgat tgaatcccca agctctatct gttcaaatca
    14701 atggaacgaa tatcgggcaa acaaatgagt tgtctattca aaaagcagta gatttcttct
    14761 caaacttaac gttatctgaa caagagaaag taattgccaa accgatttta aaagaagtaa
    14821 atgacagact ttcattcttg gaaaatgtgg gactagacta tctaactttg agtcgcgctt
    14881 ccggaacatt atctggggga gaagcgcagc gaatcagatt ggctacacaa attggatcga
    14941 atctttctgg agttttgtat attttagatg aaccatcgat cggattgcat caacgcgaca
    15001 acgatcgact gattggctca ttaaagaaaa tgcgtgatct tggcaacacc ctcgttgtag
    15061 tcgaacatga tgaagatacg atgtgggcag cagattatct gattgacgtc ggacctgggg
    15121 ccggagaaca aggcggacaa atcgtggctg caggcactcc tgaagaagta gctaatgatg
    15181 aaaattcttt gacagggaaa tatctttcag ggaaaaaatc aatccctgta ccaaaagaac
    15241 gccgtaaagg taatgggaaa gcaatcaaga tcactggtgc ttcagaaaac aatctgaaaa
    15301 atattagtgt tgaatttcca ttaggagaat ttgtagcagt cacaggtgtg tctggttctg
    15361 gaaagagtac attggtcaat agtatcttaa agaaatcgtt agcgcaaaaa ttaaataaga
    15421 attctgctaa gccaggtaaa ttcaagacaa tttccggcta cgaaagtatc gaaaagatca
    15481 tcgatatcga tcaaagccca atcggccgga cgccgagaag taatccagcg acttatacaa
    15541 gtgtatttga tgatatccgt gggttatttg ctcaaacgaa cgaggcaaaa atgcggggtt
    15601 ataagaaagg gcgttttagt ttcaacgtaa aaggcggtcg ttgtgaagct tgtcgcgggg
    15661 atggaattat taagatcgaa atgcactttt tgcctgatgt ctatgttcct tgtgaagtat
    15721 gtcatggcaa acgatataac tctgaaacat tagaagtgca ttacaaagga aaaagcattg
    15781 ctgatatttt ggaaatgaca gtagaagatg ctgtagaatt cttcaagcac attccaaaga
    15841 ttcatcgcaa actgcaaacg attgttgatg ttggcttagg ttatgtgacg atggggcaac
    15901 cagcaacgac attgtccggt ggtgaggcac aacggatgaa acttgccagt gaattgcaca
    15961 aaatctctaa tggaaagaat ttctatatac tagatgaacc aacgacagga cttcatagcg
    16021 atgacatcgc ccgcttgttg catgtattac aaagattagt agatgctggt aacacagttt
    16081 tagtgattga acacaatcta gatgtaatca aaacagcaga ttatatcatt gatttaggac
    16141 cagaaggtgg agaaggtgga ggaacgatcc ttgcgactgg aacaccagaa gaaatcatta
    16201 acgtaaaaga aagttataca ggtcactatt tgaaaaaaat aatggtataa agaggataga
    16261 tagctgagat agttaaaaaa acataaaaaa atgaattttt gcttgtttta ttaatatcag
    16321 tgttctagaa tcattacatg gtagttcttt ctacactgat tggtatagac aaaaacttcc
    16381 tataagttca atccgttcaa aaaagaattg agcaatcgga ggagtttcct ttgattgctt
    16441 ttttctttat ggtatgatat tccttgtgta gttggaaaag aaaaacaaga gattatgagt
    16501 aagtgagaga taatgatgaa cagtattgaa aatgaagaat ttatccctat attattagga
    16561 agcgacatga acgtttatgg tatggcacgt tcttttaatg aagcatacgg caaaatctgc
    16621 caagcatatg cttcggatca gttagcccct acccgctaca gtaaaattgt caatgtagaa
    16681 gttattccag gatttgataa agaccctgtg tttatcgaga cgatgcttcg tttggcaaaa
    16741 gaaagatatt ctgataagag taaaaaatat cttttgattg cttgtggtga tggatatgca
    16801 gaattgatct cccaacataa acaagagctg tcagaatatt ttatctgtcc atatattgat
    16861 tattctttat ttgaaagact gatcaataaa gtaagctttt atgaagtttg tgaagaatat
    16921 gatcttccgt atcctaaaac attgatcgtc agagaagaaa tgttagtaaa tggacatttg
    16981 gaacaggaat tgccttttga attcccagtt gctttgaaac ctgcaaacag tgtggagtat
    17041 ctttctgtcc aatttgaagg aagaaaaaaa gcttttattt tagagactag agaagagttc
    17101 gatttaattt taggccggat atacgaagcc ggttataaaa gtgaaatgat tgtacaagat
    17161 tttatcccgg gtgatgatag taatatgcgt gtgctaaatg cgtatgtaga tgaagatcat
    17221 caagttcgga tgatgtgtct tggtcatcca ctgttagaag atcctacacc agcatcgatt
    17281 ggtaattacg ttgtgatcat gccagattat aacgaaaaaa tctatcaaac cattaaggca
    17341 tttttggaaa aaatcgaata tacaggcttt gccaatttcg atatgaagta tgatcctaga
    17401 gatggagaat ataaattatt tgaaatcaat ttgagacaag gtcgtagcag tttctttgtg
    17461 acattgaacg gcttgaatct ggctcgtttc gtcacagaag atcgagtgtt taacaaaccc
    17521 tttgttgaaa cgacctatgg aacgaatcaa tcagataaag caagattatg gatgggtgtg
    17581 cccaaaaaaa tctttttaga atatgctcgg gaaaatgaag acaaaaaact agctgagcaa
    17641 atgatcaaag aaaatcgtta cggtactact gtgttttatg aaaaagaccg gtcaatcaag
    17701 cgctggttgt tgatgaaata tatgttccat aactatattc caagattcaa aaagtatttt
    17761 catgtgaaag aaggctagtt catggagaat tttttcagta ttttaggcgg aatgggcacg
    17821 atggcgacag aaagttttgt tcgcttgatc aaccaccgaa cgaaagctac aaaagatcaa
    17881 gaatatttga attatgtctt attcaatcat gcaacagttc ctgatcgtac agcttatatt
    17941 ttagatcgat cagaagaaaa tccaatgccg tttctgctgg atgatattga gaaacaaaat
    18001 ttattgcagc cgaacttcat tgtgttgact tgtaacaccg cacactactt ttttgaagaa
    18061 ttacaagcgg caacggatat tcctatcctt catatgccaa gagaagcagc aaatgaactt
    18121 gtccgtcagc atacgacagg gagagtagcg attctaggaa cagaaggaag tatgaaagct
    18181 ggaatctatg aacgcgaagt caaaaatctt ggattcgaaa cagtgattcc tgatactgtt
    18241 cttcaagaaa aaatcaatta tctgatctat catgagatca aggaatcgga ttatttgaat
    18301 caagagctat attacgaaat cttagaagaa gcggtagagc gcctaaactg tgaaaaagtt
    18361 atcttaggtt gcacagaatt gtcattgatg catgaatttg ctgaagataa ccattatcca
    18421 gtgatcgatg cccaatctat tttagcagat cggacgattg aacgagcgct agctgaaaga
    18481 agcaaggcat tggatacagc atcggaaaag taaaaaaatt ggatttgggg caaatatcct
    18541 aaaagatatt tggtccaaat ctttttgtaa aagaaaaaga aaactggtaa tgatacgccc
    18601 ggaatgatat aattttacta tgcacaatgt gagaggagtt cgtaattatg acagataatt
    18661 tgcaattagt catcataaca ggtatgagcg gtgcagggaa aacagtagct atccaaagtt
    18721 ttgaagatat gggctatttt tgtatcgata atatgccgcc tcgattgatt ccaaaattct
    18781 gggaattaat caaagaatca ggcaaagtca caaaaatggc tttaggagtg gatttacgtt
    18841 ctcgttcctt ctttgaagaa atccaaaaca tgctgatcga aatcgaaaat acgaacttca
    18901 tcgacacttc tatcttattt ttagatgctt cagacgaaga actagtggct cgctacaaag
    18961 aaacaagacg agcacatcca atggcgatgg acggtttgat cactgaaggg atacgtaaag
    19021 aacgggcgat actagacgac ttgaaagccc aagcttccgt gatcattgat acgacgacat
    19081 tgacaccacg acaattacgt gaaaaaatca atgagtcatt caaatcaaag ggagaccaag
    19141 gatttcggat cgaatttgta tcatttggtt ttaaatacgg gttgccgatc gattcggata
    19201 ttgtgatgga tgtgcgtttt ttgcccaacc ctcattatat cccggaacta agaccattga
    19261 ccggaaaaga tcagccagtc tatgattatg tgatgtcctt tgatgaaacg caatcattct
    19321 accataaatt tttggatctt ctggaaactg tattgcctgg atacattaaa gaaggaaaaa
    19381 gcagcttgac agttgcaatc ggctgtacag gcggtcaaca ccgttctgtg gcattgactg
    19441 aacgtttggc aaatgcttta gggaaaaact ataaagtcaa tgtgacacat cgggacaagg
    19501 ataaaaggaa agagacggtg aaccggtcgt gaaaatgaaa acctaccgga taagaaaacc
    19561 aaagatcgta gtggtcggcg gtggcactgg tctgccagtt atcctgaaaa gtttgcgaaa
    19621 tcaaggagca gatatcacag cagtagtgac tgttgcagat gatgggggaa gcagcggtgc
    19681 aatcagagaa tcgatcgcta tggctcctcc tggtgacttg agaaatgtct tagtggcgtt
    19741 atcggatatg ccacaattct atgaagatat tttccaatac cgtttcaaaa aggaagatca
    19801 gtttttagcc aatcatacga tcggtaatct gatcattgcc gcagtttccg agatgcgggg
    19861 aagcacttat gaggcgattc aattattagc aaaaatgatg catgtagatg gacatgttta
    19921 tccttcttca gaaactcctt tgaccttgca tgctgtcttt aaagacggaa cgactgcagt
    19981 aggagaatct aaaatcgcga tcgaccgtaa aaccattgac cgtgtgtatg tctgcaacac
    20041 aaatgataaa agtgaagcca aagcggctcg aaaagtcgtt tcagcaatta tggacgccga
    20101 catggtcgtt ttgggacccg gaagtttatt caccagtatc cttccaaatt tagtgatacc
    20161 agaaatcggc gaagcaatgc tcaagacaac tgctgaaacc gtctatatct gtaatatcat
    20221 gacccaaaaa ggggaaacgg aacatttcac agatgccgat catatacgtg ttttacacaa
    20281 gcatttgaat cagcctttta tcgatacagt attggtcaat acggaaaaag tgcctgaaga
    20341 ttatatggac ccagagatct atgatgagta tctagtacaa gtcgcacacg acttcaacgg
    20401 attgagaaat gaaggatgtc gagtgatttc caccgacttt ttggaattga aagatggtgg
    20461 tgtcttccac gatggagaca aagtagtaga agaattgttc cgtttagttt tcggttcaaa
    20521 atattaagca atttattgtt ggagaaagga ggagaatagg atgtcatttg ctgcagatgt
    20581 gaagaaagaa ctaactggtt tggaagttca tcgagaacat gcgaaagcgg aattagctgc
    20641 tttgctgcga atgaacggct ctttaggtat tgtgaaccag cagtttattt tgaacgtcca
    20701 aacagaaaat gctgcaattg ccagacgtat ctattctctt ttgaaagatc attatcaggt
    20761 acgaagtgag ctactcgttc gtaaaaaaat gaaattaaaa aagaacaatg tctatatcgt
    20821 ccggttgaag caaggcacaa aagatgtgtt gatggatctt gatatcatgg atggtatgat
    20881 gttcaatgga cacgtttcgg acgagattat gggaaattcg caaaaaatgc gttcttactt
    20941 acgtggtgct tttatggcct caggttcagt caataatccg gaaacgagcc gttatcattt
    21001 ggaaatattt tctatttatg aacagcataa ccaagacatt tgcgatatgt taaattatta
    21061 cggtctacat gctcgaacgc ttgaacggcg caatggctat atttcttatt taaaaggagc
    21121 agaaaaaatt gctgatttct taacattgat tggtgcaacc aactcaatgc tccgatttga
    21181 agatgttcga atcgtacggg atatgagaaa ttcggtcaat cgtctagtca attgtgagac
    21241 agcaaatctg aacaagacga ttgatgcggc ttctaaacaa atcgaaaaca tcgaattgat
    21301 cgaagcaaag gtcggtctac atgcccttcc tgaaaaactt caggaaatcg cagaacttcg
    21361 gcttcaacat ccagaggtca gcttgaaaga attaggggaa atgattccct caggtgcgat
    21421 atcaaaatcg ggaattaatc accgaattcg caaaataaat gaatttgcgg aaaaactgcg
    21481 tgaagaagca gtttaatcca aagaaaaaac tgatacatga agcaggagat gcgaatgtat
    21541 cagttttttt taaaagttat acttgctttt cttccgtcaa actataattc catctttact
    21601 ggagtaacat aatttggaaa ggcttctatt gtttttaata caatcgtata aaaagcgggg
    21661 agacaactga atgaagattt tagttttgta cataaatttt tgcttaatat agaagtcctg
    21721 atatagataa gatcaagtat ttatctataa ctggcctgat taattaaaaa actggaagga
    21781 actgctcgat catccttgag aactgctctt cttacttctg gtcaagattc tcgtggttgg
    21841 tggataagga tggttgataa gttcattgga ttagctagtc gaaaacaacc aggtgtaatt
    21901 aatgtagaag ttttaacaag gggatggagt gatttattca gtgataatca taagttcata
    21961 aatagtaatt gagatgtatg gttggaatac gttatacctg aacttgaact accaagattt
    22021 ggtttagaat ttgagaatcg atgtttataa agggagggca aaaatgcgtt tttttacaac
    22081 ttttactatc attatgattg cagtactttt tatttttcta gatatagcaa agcgaaatac
    22141 agcattttta ttatatcggg ttttacttcg agcaggtctc ataacattta tatcgatcgt
    22201 tggatttttt ctattcacag ttattgtatt tatttggaga actccagcac caccattgcc
    22261 agagattaca tatggagaat ttccttttag acttgagtat gaattgaatg aagaattaca
    22321 tgtgatagaa gacacgttaa tagttgaatt tgatggattt ggaatgaacg aaggcatagg
    22381 gagatatcgt agatggacaa gtcgtttagc cagcggagaa gatttagtgt tgctgttaga
    22441 agttagtgat aataagcaaa tattttattt tccaggacct gctaattatt acatgggaga
    22501 taggttgaat ggttataatc atacttttcc tagtgcttct ttcattgaaa gggaaagagg
    22561 cattactaga agggatattt tacatgacaa agaattatta gaacaatttg gtccgcttga
    22621 tcaaaataca ataaatgaag aagaattgtt aaatcaatat aatataaggc tagtgaattg
    22681 ggaaatatct gaaccgattg ttaacaattt tggtgattag aaaacaatta cgataaatag
    22741 aaacaagcca aatcatcagc aagtaaaagc tgatgatttg gcctttctta tacgagtagt
    22801 aggaattcat agcgaatatt attacgatca gtagatattt tgatattaaa ggagtattac
    22861 gaactagtat gtgagaatgt agaggtgatt tgcgaataat gagaaattta tcaaatagaa
    22921 acaaaatatt aatcattatt gttgttatag ctgtgtttca tctgggaacg aatgcggttt
    22981 taagtagaat aatattgggt cctaagccac caagacctgc gattacccga ggggagtttg
    23041 attttagatt agaatatgaa gtagatgggg aaaggattgt gatagaagat acaattgttg
    23101 cattatttga tggattttct gcagatgcag gtagcatggc atggtatcgc acgtggcgtt
    23161 tgcatttggc gagtgacaag agggcacaac aattattgtt acatgagtta gatgatggtc
    23221 gaaaaatata ttatcatttt ggaggagctg gattttatat ggggaagtta gtagcagtgt
    23281 aactgtaaca aggaaagatt tgccaggtct tggcattcga aactccatat tttatatcga
    23341 gcgtgggaga gatacatcta ttgggaacag gaaagggtta actcttgaag agttatataa
    23401 tgactttgga atccgtcttg ttagttggga acacaatcca ccaatagaaa atcgctttga
    23461 ataatcgaaa tctattaatc catttcatca gccagtgaat gttgtaatgg aacaatttgg
    23521 tttagtgttc aaaaccattg aaatactagg ctgtgatcag caaaaatgta tctgaataaa
    23581 aaagagagca actattaaat gagaggagtt aggagttgtt agctttatac gaatcaaagt
    23641 ggcaaataca gatgcgtatg catttgtgtt tgccatcttg cgtttggtag acgagatagg
    23701 tataagctaa agaaacaact tgtttaaaat aaacgaccta gctcaacaaa cgaccatcta
    23761 atcaactaac agcatacata aaagcaacta tttttgaagt agaattgaaa tcgggataac
    23821 ttaaaaagtg attgggatac tagtgtgaat caccgtacat aaatattact cgttaaggga
    23881 atttgagaaa ttttgtatga aaatttaaga attagttagt atgcgcaagt caaaaatatg
    23941 aataaattat ttggtggaga taatttgctc agcttgtcat aagaaatgag ataatctggc
    24001 agatgcaagc ttttggacac catttattcg gaataaggag gctgggacag aagcgtttaa
    24061 ctccaagaaa taagaaggag ttgcttctgc ttccgccgtt tataggtgtt tagagcgtga
    24121 gacaaaagtg ttctttactt ttgtcctacg ctccttattt ttttattttg tttggatcaa
    24181 ttcttttaat tcctctattg aaatagattc atcaaagatt tctttaccca agatgacagt
    24241 cggaacaaat cgaatatgtg cttgctcagc ttcgttgatg acagcttggc tcagctgttc
    24301 gtctttttgt tctgtcagct ttagtttatc tactgcaaaa tccgctactt cttggagaga
    24361 aagatgtttc cactcgtctt gagtatcgaa tatttgtttg atctctttga tcgctttttg
    24421 accatcgctg attgtgagat accggtgcat cacgtttcct cgctgcaagc tttctttttc
    24481 tttatcgaac aatttgatca ctcgctgcaa ttgcccgctt tggactgctt cttctaatag
    24541 ctcataagat tcttcaaacc attgtcggca atacggacaa gataggttga tgaattcgac
    24601 cattttttta ggagcattcg atgaaccata aaggattcct ttttgtgtat ttgttttcgt
    24661 tgcatcaatc actgatatat ccatgttaaa acacctcttt cgcttatagt ttatacatgt
    24721 ctgacaaaaa aaggcaacga tttagactgt ttgtctaaaa attagatgac caattcaaat
    24781 agacatatgt gaaatatttt tataaataaa agaaaaaatg gaatgtttta cgttataatg
    24841 gaacagataa taagagagaa tatgaacaag gatgatgaat catgcgaaaa atgaaaacaa
    24901 tggatggcaa tacggcagcc gcatatattt cttatgcatt taccgagatt gctgcaatat
    24961 atccaataac acccagttcg accatggctg aattagtaga tcaatggtca gcagcaggaa
    25021 aagaaaatgt attcggtcaa ccagtaaaag tagtagaaat gcaatccgaa gcaggagcag
    25081 ctggtgtggt ccatggatca ttgaaaactg gtgcactgac aactacatat accgcttctc
    25141 aagggctgct tttgatgata ccgaatatgt acaagattgc tggggaacta ctgccaagtg
    25201 ttttccatgt tgctagtcgg gcagtgacga caaatgcgtt gaatattttc ggagatcacg
    25261 gagacgtgat ggctgcaaga caaacaggat ttgctatgtt agctgaaagc agtgtacaag
    25321 aagtgatgga tttagcacct gttgcccatc ttgcttctat tgaaggagaa gtaccttttt
    25381 tgaatttctt tgatggattc cgtacgagtc acgagattca aaaaatcgaa gttttggaat
    25441 atgaagaatt agctcctctg atcaatcagg agaaattagc ttcatttcgt aaacgaggga
    25501 tgaatcccaa tcatccgact gttagtggta cgaaccagaa tccagacatc cattttcaac
    25561 aacgggaaac gatcaacccc tattatgata aattacctga aatcgttcaa aaatatatga
    25621 aagagatcaa ccgattacga ggaacttctt atgacttggt gacttattac ggagcagacg
    25681 atgcggaaga agtcattgtt tctatgggat cagttgcaca gacgattgaa caaacgattg
    25741 attacttgaa tgcaaacgga cggaaaacag gttttttgaa tatccatctt tatcgtcctt
    25801 ttccagtgga aacattcgtg caaaagttgc ctcatacagt aaaaacaatt gctgttcttg
    25861 atcgaagtaa agaaccaggg gcaggtggag aacctctctt attagacgtt caaagtgcgt
    25921 tatatgattc tgaacttcgc ccggctgtta ttggtggtcg ctacggattg gggtctaaag
    25981 atgtgactcc cgaccaaatc tcagcagttt ttgatgaatt gaaaaaagac ccaagcatac
    26041 gcaaaaaacg gtttaccata gggatcgtgg atgatgtgac ttatcaatct ttggaaccaa
    26101 aggaaagtct ggatttgaca gaaccgcaaa cattccaggc caagttttgg ggatttggat
    26161 ctgacggaac agttggagca aataaatcag ctatcaagat tattggagat cacaccgata
    26221 aatatgcgca aggctatttt tattatgatt cgaaaaaatc tggcggtctg actgtctctc
    26281 atcttcgttt tggagatacg ccgatacgtt cagcgtatct tgtcgagcat gcggaccttg
    26341 ttgcctgtca cacaccagct tacctgcata gctatgactt ggtcaaagga ttgaagccag
    26401 gtggcatttt tttattgaat actctttgga gcgacgaaca attagaaacg catctgccct
    26461 taaaattaaa acgttacttg gctgaaaata acattagatt ctatacgatc aatgccatga
    26521 gattggccca agaagtcggt ttgggacgcc ggatcaatac ggcaatggaa acggcttttt
    26581 ttaaattagc tgacatcatt ccttttgatg aggtgcttcc gctgctaaaa gaagaagccc
    26641 tgaaaagcta tggacataaa tcaatggcta tcgtagaaaa aaacatacaa gcaatcgatc
    26701 ggacgaccga actgcttcat caagtcgcga tcccagacca ttggagaacg ttggagatgc
    26761 ctgaaaaaat caataaaagg aacactggtt atatacaaga aatacttgaa ccagtgaatg
    26821 cacaggaagg aaatcaactc tctgttggta cacttgtagc aaatggaatg acaactggcg
    26881 aaatgccttt aggaacggct tctatggaaa agcggggcgt cgctttagaa gtacctgagt
    26941 ggattagtga tcgctgtacg atgtgtaatg aatgtgcctt tgtttgtcct catgctgcca
    27001 tccgtccatt tttagcagat gaagaggaaa tggaagaagc gccagaagga tttatcgtga
    27061 gagaaatgcg aggcgcagac ggcgtaaaat accgtatcca agtttcagtg gaagactgta
    27121 caggttgtgg tctgtgtgta gaggcttgtc ccgcaaaaga aaaagcttta gtgatgaagc
    27181 cttatgaaga acagaaagaa caagctgtga attgggcttt tgcaatgaca ttaagacaaa
    27241 aagcaaatcc agtcaaagga aagaatactg tcttgtctac acaattcaat caaccgcttc
    27301 ttgaattctc gggcgcttgc tcaggctgtg gcgaaacacc ttatgtaaaa ttattgacac
    27361 aaatgttcgg tgatcgaatg atgatcgcga atgcgacagg ctgttcttct atttggggcg
    27421 gtgcttctcc tgcttctcct tatacgacca atgaatgtgg tcaagggcct gcatggtcaa
    27481 attctttatt ggaagataat gctgaatttg gttatggtat gcttctagcg gcacagactc
    27541 gtcgcgaggc tttggcaagt tcaatcaaag aagtattgcc aaaagtatca gaagaactac
    27601 gagaattgat ggaagactgg ttggtacaca tgtttgagag cgaaggaacg cagcaaagag
    27661 cagcaaaact gcaagctgcg atcgaaagcg aacgatcaat tgtccctgaa ctggaggata
    27721 tatacaagag aaaggatcta ttcgtaaaaa acagccaatg gatgattggt ggagacggat
    27781 gggcttacga cattggtttt ggcgggatcg atcatgtact tgccagcgga gcagacgtga
    27841 atattcttgt tttggataac gaagtctatt ccaatactgg cggtcaaacc tcaaaagcaa
    27901 caccagcttc ggcaatcgct aaattttcgg ctagtggaaa gtatgcttca aagaaagatc
    27961 taggaatgat ggcgatgaca tatggaaatg tttatgtggc gcaaattgct tcaggtgcca
    28021 atcaaatgca gacgataaag gcttttgaag aagcagaaaa gtatcctgga ccttctttaa
    28081 tcattgccta tacgccttgt atcacacatg gtttgcttgg tggaatgaaa gaatcgctta
    28141 aagaagcaaa agaagctgtt tcttctggct attggtcatt atatcgatat aaccctgcaa
    28201 tggtcgaaaa aaacaagaac ccaatgactt tggactacaa aaaacctgat ttctctgcta
    28261 tgcctgattt catgaggaaa caagttcgct tttcctcgct agagaacgca aatccaaatc
    28321 ttgcaaaagc tttatttgaa aagactatcc aagacgctaa aacacgtttt tacaactatg
    28381 ctgcactgac gggagaatta gaaaaaatca agaaacgact tgaaccagag gaatctatcg
    28441 atgtacccgt taagactaga aaagaaaaaa cgacggacct tgaagcagaa gaaagaagag
    28501 ctgccagagc agaacgacgt aaaaaaatgg aacaagctac aaaagaaaaa aaggaaaaat
    28561 aacagaaaaa atataatgaa taattctctg aagtttaaaa atatttattg cagagaaaaa
    28621 taaaacgcat acttgcaatt ttcctgaaaa ttatgcgttt ttctcaagaa aaatacgagc
    28681 agatcagcct ctaaaattaa aacatatgat atactagaga ggaaatctca aaagagaaga
    28741 gaggcttttt atgtcatttc aaatcgggga actttttaat ttggactatt ggcagcaaat
    28801 gttttccggt gacttgttct ctctgaattt tttggtgaac atacttgata ttctggttgt
    28861 ctggtatctt gtatacaaac tgatccaact gctgcgaggc acaaaagcga ttcagcttct
    28921 gaaaggtgta gctattttta ttgttatccg aatcgtagcg gaaatcattg gtctgcacac
    28981 cttgtcttgg ctgatgaacc aagtcattac gtacggggtg atagctgcta tcgtgatttt
    29041 tcagccggaa atccggagag gactggaaca tcttggacgc agttcgcttt tcaaacaatc
    29101 aaaaagcgaa cagcaggaag atgagaagat gatcttgtct tttgacaaag ccatccaata
    29161 tatgtccaag cgaaaaatcg gtgcgctaat tactatcgaa cggcatactg gattagatga
    29221 gtatattgag acaggaattg cattagatgc agatatcact ggagagctgc tgatcaatat
    29281 ctttattcct aatacaccgc tgcatgacgg tgcagttatt gtaaaagaag gaaagatcgc
    29341 agtagcaagc gcctatcttc ctctatcaga aagtatgttg attccaaaag aattcgggac
    29401 tcgacaccgg gcagctgtcg gtataagcga agtcagcgac gcgattacca tcgttgtctc
    29461 tgaagaaact ggggatgtca gcattacatt agataatgaa ctgatggctg gcttatccca
    29521 gcaagaatat ttggcaattt tgcgtagaga actgattgct gaagagaaaa caaaaaacaa
    29581 gaaaaacgtg attcaatctt tcctagacgg tatgactaag ggaggggatc gcaaatgatc
    29641 tcaaaagaga aacggacaag cattctttac agttttattg cattgctgtt cagtattgcg
    29701 ctgtacttca atgcaaatgg ccagtccgtt ccaaatacgc tttctggaaa tgaagcgtat
    29761 aatcaaacag ttactggggt gccaatcaag atttcttatg attcaaagcg ctactatatc
    29821 catggatatg aaaatgcagt gactgtcaaa ctttccagtg caaaccgagt tcagttgaat
    29881 gcagaggcaa acgaagatac acggatgttc cgcgtcacag cagatatcac taagctgggc
    29941 gaaggaacac atgaggttcc gctaaaaatt caaaatttga gcagtgctgt cacagcaaag
    30001 atcgaaccat cgactattac agtaacggtt gagaaaaagg taataaaaga tttcgatgtg
    30061 gaaacgttga ttccttcaac gttcacacca tctggatatg aacttgatga tacttccgtt
    30121 agtcctaaaa aagtctctat cactacaggt gacaaaacat tggcagaaat caaacgagta
    30181 gtagctgatg tggacgcttc gatggtcaca gatgatggga ttaatagtga ggtcccaata
    30241 caggcgctga atgcagcagg ggaagtatta agtatcgtct ctgatccagt gcaagtaaca
    30301 gtgagagctg attcgatcaa gccaagcaag tcggtacgac tttatgggat acagcaagga
    30361 acaccagctg ccggggtaga aagctatgat ttcagttttt caaaactgga agcagaagta
    30421 agcggctcta gcgatttgct tgcatcgatc ggtgacagta ttgctgtacc gatcaatgtc
    30481 tcaggtatca atcatcgaac aactcgtaaa attgaaatac cagtagaaga ggggttaagt
    30541 atcaagcccg aatcagttag tgtggagatt acacccgtgt tgcaaacgtc aagcagtagc
    30601 tctgcttcga cgagcagtcg gacacaagaa aatacaagta cgagtagaag tatttcatct
    30661 gctgattcag gaacggaacc atcgacaaca accatttctt ccagttcgga atctgggaca
    30721 agcagctcga ccacaagtac agaagaaaca tcagaaacaa gcgaatcaac acaaatgagc
    30781 agaacaaatt cctcagaaaa ttaagttaaa ggagtaattt tacaactatg ggtaaatatt
    30841 ttggaacaga cggcgtacgc ggtgaagcca acaaggaact tacgccagaa cttgcattta
    30901 agctgggacg gtgcggaggt tatgtcctaa gccagcatga agaagaagga aaacgcccac
    30961 gcgtgttagt agggcgggat acaagaatct caggtcaaat gttagaagaa gcgctgattt
    31021 caggactttt atcggtgggt atcgaagtgt tccagctagg tgtgatctct acaccaggtg
    31081 ttgcttatct gacacgtttg caaaaagcaa gcgcaggggt catgatttct gcttctcata
    31141 atccagcaca agataatgga atcaaatttt tcggtgcaga tggttttaaa ctagtagatg
    31201 accaagagct agaaattgaa gcattattag atgctgaagt ggatgaattg cctcgtccat
    31261 cagctgaagg attgggaact gtagaagaat ttccagaagg cttattgaaa tattctcaat
    31321 tccttgtaca aacgatcaac ggtgaccttt ccggtttgac catttgtgtc gatgcagcaa
    31381 acggagcaac ttcaacaagt gttaatcgtt tgtttgccga tcttgaaaca gatttttata
    31441 ccatgggcac aaatccagac gggttaaata tcaatgatgg cgtaggctct acccatccag
    31501 aacgtttagc tgagatggtc gttgaaaaag gagcagatgc tggactagct ttcgatggcg
    31561 atggcgatcg tattattgcg gtggatgaac ttggaaacat tgtcgacggt gataaaatca
    31621 tgtttatctg tgctaaatac ttggcagaaa aaaatcgatt gaaaaaagat acgatcgtta
    31681 cgactgtcat gagtaactta ggattccaca aagcggtgga aggaatcggt ttgaaagatg
    31741 tcgttacaca agttggtgat cgttacgttg tagaagaaat gcgcaaaaac gattataact
    31801 ttggcggcga gcagtctggg cacatgattt tcttagattt taacacaact ggagatggga
    31861 tgctttcagg aattcagtta ctgaatatca tgaaacagac aggtaagaaa ttatcagagc
    31921 ttgcagcaga ggtaacaatc tatccgcaaa aattagtgaa tatccgagta agtgataaaa
    31981 acggtgcgat ggatgttccg gcaatcaaaa aagtgattga agaggcagaa gctgaaatgg
    32041 gatcagaagg aagaatccta gttcgtcctt ccggtacaga accattactt cgagtgatgg
    32101 cagaagcgcc tactgatgag aaagttaatt actatgtcga taaaatcgcg gcagtagtca
    32161 aagaagaaat cggactggat taagagatat tttcataaaa aataaaagaa caaggaccag
    32221 tgtcagatgt ctgacactgg tccttgttct tttattattc tactgtcact gatttcgcta
    32281 aattacgtgg tttgtcaacg tcgaatcccc gtaatagcgt tgcgtaatat gcaagtaatt
    32341 gagtcggtac gactgtggtt agagtagtta gataagggtg gacagttggt agcaccagct
    32401 ggtcactttc cttttctaat ccctctgaga caataactag tgttttagcg ccacggcttt
    32461 cgacttcttt caggtttcct cgagtatgcg caccagtcac ttcttcactg atgattccaa
    32521 tcactggtgt tccttcttcg atcaaagcaa tcgttccatg tttcaattcg ccagcggcaa
    32581 aaccttctgc ctggatataa ctgatttctt tcaatttcaa agcagcttca agagaaacgt
    32641 tgtagtcagc gccacgtcca atgtaaaagg catttcttgt atcgctgaga tatacttctg
    32701 ccaactcttc taaataatct ttttgggcga tgaccgattc catcgcatta gcaactaaac
    32761 tcaattcatg agtgatatcg aatacaagag aagtttcaaa ttctttttta tcaccgatag
    32821 ctttggctaa taaagttagt acagctattt gtgcagtata agctttcgtt gatgctactg
    32881 cgatttcggg tccagcatgc aatagcagtg taaaggatgc ttcccgcgaa agagtagaac
    32941 cagcaacatt tgtaatcgta agtgaagggt agccgagacg gttgattttt accagaactt
    33001 ggcggctatc tgctgtttcg ccactttgac tcaagaaaat aaagaatggt ttttttgaaa
    33061 gaagaggtgt gttatagcca aattcactgg ctaaatgaac ctcaacaggg atctgtgtca
    33121 atttttccag agtctgttta cctgctaacc ccgcatggta actagttccg caagcaacga
    33181 tatagattcg atcgctatca gacaatgtat cgaccagctt ttgatcaaga gagacacgat
    33241 tgtcttctgt tacgtatttt tgagcgatac gacgcatgat aataggctgt tcgtcgattt
    33301 ctttcagcat ataaaaagga taggtacctt tctcgatatc tgaagaatct acttgtgctt
    33361 cgaatgaata acgttctacg atgtcacctg caaaattttg aataataact ttttctgatg
    33421 tgacggtaac catctcttca tcttcaatct caacgaattc cttcgtatga gcaagcatag
    33481 ccattgcatc gctggcaatc acattgaatc catcgccgag tccgattaat agtggacttt
    33541 tatttttagc gacgtaaata acatcaggtg ctgtccgatc aatcaaagcg aaagcgtaag
    33601 aacctttgat gacccgcaaa gcttttaaaa acgcctcttg cgttgtagta tccttcgcga
    33661 aagtttcaat tagatgagcg ataatctcag tatcggtctc accaataaag tggtcatttg
    33721 ctaaataagc aattttaagt tcttcaaagt tttcgattac gccattgtga acgagcacga
    33781 agcgtccact ttgggaagta tgtggatggg cgttttcttc agaaggttgc ccgtgagttg
    33841 cccagcgtgt atgaccaata cctatatttc ctgtagtatc aatatttatt ttgtttttca
    33901 aattttgaat tctgccttgt gctttgacca gataatcagt tgtgccatct gagacaaaga
    33961 taccagctga atcataacca cggtattcta atttttctag accgtcaatc aatcctggtg
    34021 ttacattttt taatccaatc attccaacaa ttccgcacat aaaaaacatc ctactttcta
    34081 taatgaattc gtatgaatgc ttccagctgc aggaaaatcc ttttgtttta tctttgcaga
    34141 tattttttgt ggattttctg tagagtgcag tccctagggc catccgccga atctttcgat
    34201 aaaccctctc ctcgtcatct gtttacacag acctggcgct atcctaaaag gtataactat
    34261 cctcctcttt tgttttttat ctggaaacat ttaaaatcat aaccttttgc gaatgttctt
    34321 gtcaacaaaa aaagaataaa aaataaattg gtatatatca aatgctttgt caaatgctct
    34381 ttttatgtca aaaaataatg ggtttttatc tatcatcttt ttcaggagtt cggtacaata
    34441 gaaagtaatc ggatagagga ggagtaaaaa tggattcaaa aattgagatc atgacactgg
    34501 gaatgttaaa aaaacaattg tccaaattcg aagcatcagc tggtgtatca gatgatacga
    34561 aaatattttt agatactggc tgggatagca tccaagaaat cgcacctgat gccttggaag
    34621 ttgctgaagc tcgtgagttt acggtagaag atgaattgac aaaagagtcg ttttccgggt
    34681 atgcgcggga agaaaaagca gagcggttcg acacttctga gcagtcagaa actgtgatcg
    34741 tcataaaaaa cttgtattag aaaggagaaa gaatgaataa gagacgttgg gcagcagttc
    34801 tgattgctgc aggattattt gtggtttcgt tagtttcagc tagtttgact aagcctcagg
    34861 aaaacgatca aattgaggga ttgaacagtt ggttatatgg ggatgaagaa ctagcttcaa
    34921 atgtgttgga agagggagac agtacgcaaa aaatcgtaaa attatcagta gatggaacga
    34981 ttgctgcggg agggtcttca ggtttatttg cttctgaagg ttacaatcat aaaaatttta
    35041 tggaacaact gaaggcgatt gaagaagatc caacagtcaa aggtatcttt ttagaagtaa
    35101 attctcctgg tggcggtgtc tacgaaagtg cggaaatcgc taagaaattg gatacgattc
    35161 gtaaagaaca tgatatccca atgtatgtca gcatgaaaaa catggcagct agcggtggct
    35221 actatatttc tgctcaagca gataaaatct ttgcaacaga agaaacagtg accggctcaa
    35281 ttggtgtgat catgtcagga ttgaattatt caggacttct agaaaaactg ggtgtggaag
    35341 atacaacagt taaaagcgga gcgttgaaag atatgggttc aggaacgcga ccagagacaa
    35401 aagaagaaga agcggtgctt caagcatata tcgacagcgc atatagtcgt tttgtagcaa
    35461 tcgtcagcga gggacggaat aaatctgaag aagaagtcaa aaaaattgct gatggaagga
    35521 tttatgatgg tgtccaagca aaagaagtag gtttagtcga tgaattggga tttcctgaag
    35581 atgctttaaa agctatgcga aaggaacaac agcttgaaga tgcggaacta gtagaatatt
    35641 cttccacgcc atctgggttt gctagtacat ggttaggaag taaactagca gaattccagg
    35701 gattgaagcc ttctgagact agtcagatct tatccgttct tgagaatcta gggacagcgg
    35761 aatcaccaaa agcaatgtat tattacggag gtgagtaagc atgtcagagc tttcaaatga
    35821 aaataaggaa caagaaaaag ataagcaaca gcagcctgct gtaggacctg actccaactt
    35881 ttcccaaaag gtactgagct cttttgaaag agagcctttg acgcccgaag aaatcaaaaa
    35941 gaaacaacgt aaatggaaaa agtatacgga cacaagaaat gaaccgccaa aagcaaatga
    36001 ttttcctaag tatttctatg caggtttttt tatccgaatg tttgctttct tggtcgatct
    36061 tttgtgtatc ggagctatta cgcgtttaac acttggtatt gccaaaaatc taggttggct
    36121 taatttttca gattcttatc tgagtatcta cggattcaca gccttactta tttatctagc
    36181 gtattttatt ttattgacga aattcaatca tggtcaaacg attgggaaaa tgatctttgg
    36241 tattcgagtc atcagctttg atgaagaaga acttagctgg tcaaccgttt tagttagaga
    36301 aggtgcttgc cggttcattc taaaatttcc tttgctgatg ttagggtatc ttccagcaat
    36361 ctttaatcag aaaaagcaac atctaggcga ttttttcact aatacaagtg ttgttacgat
    36421 caatctgatc aaagcattca accaggaagt caacagctga tgagcaataa aaagcagtaa
    36481 cagttgaaag caccttctaa aagtcagatc taaaatctag attttagagg gtgcttcagt
    36541 tatttttaat tgtcatgctt tttttgtttt gctactattg aacatgagct gaacatgagc
    36601 aagaggagga cactgcgtga agccgattga ttttccagaa aaatatgaga atttaatgcg
    36661 agtagcacaa caagcactag caaaccagca atatcagcag gcaaaagaat tatttcaacg
    36721 agcttacgag ttgaaagaaa gctttgaggc taatagcctg cttgtttttt gtctttacga
    36781 gttggacgag aaaaaagaag cattgaagca agcactcttg catgaaaaac agtatcttca
    36841 aaatgaggag tttgctgaat tttattttga cttgttaatc agtacacaag attttttata
    36901 tgctagaaaa ttgatagcaa gtacagattt ttatgaagct ttcgagcagc gggtcattga
    36961 aaaaatccaa tttgcagaag aattatccgg acaaatggag aggcagaaga tcaaggcact
    37021 ccacaataaa tcggaggaac ttccttcgtt ggaacctgca cgtcagcttg cgtctattga
    37081 tgagatcgaa cagcttcctt atcatgagtt tatccaaaca gcgagtaaac ttattgtatc
    37141 gccagaggtc catattttag caagggcaaa attactggag acacttaggc aattgaacga
    37201 atgccatcct gttttctatt tgacgattga agaaaagtta gtcaaagtca taccaaaaga
    37261 tctgccaaag ccgcagcagc aggcaagtta ccagcaatta tgtgtatttt cagatcgtta
    37321 cggtaatgaa gatgcgctgc ttagttccgt gttaaatgaa gaattcacgt tgcaatctgc
    37381 aattgtttat cctgtctatg atacatacat cgaagacccg aagcgctggt ttcaactgac
    37441 tgtagaggcg tgtacaggaa aaacgatgta tgaaggtaca gaagatgaaa aacaagattt
    37501 tttaaaaaaa agagaaaaaa ttttgcagca aatgatcttc ttccattaga ttttatcagt
    37561 gaaaaagata aaaatgttat gaaaaacaag gattctatcc attcaatatg tttacaattt
    37621 gaaagggtta gtgtactatt taaaggtatg catgaaacgt ttttgatgta taaaatgaat
    37681 ttctaaactc ggagggaatt aaatgactgc aacatttgaa aaaaaaggca cgaatgatgg
    37741 tgttttaaaa tttacgattg cgcaagacga aatccaaaaa ggattaacaa ctgcgtttaa
    37801 taaagtgaaa aaaagcttga acgtacctgg attccgtaaa gggaaagttt ctcgtcaagt
    37861 attcaaccgt atgtacggtg aagaagcttt atatgaagat gcattgaatg ctgtattacc
    37921 agaagcttat gaagctgctg tcaaagaagc tggcatcgat cctgtttctc aaccaaaaat
    37981 cgatgttgaa agcatggaaa aaggtcaaga ctgggtaatc actgctgaag taacagtgaa
    38041 accagaagta aaattaggac aatataaaga tctagttgtt gaaaaacaag accgtgaagt
    38101 aacagatgct gacgtggatg cacgtatcaa acgtgaacaa gaagcacaag ctgaattagt
    38161 tatcaaagaa gatgctgcag ctgaaaatgg cgatacagta gtaatcgact tcgaaggctt
    38221 tgtagatggc gaagcgtttg atggcggaaa aggcgaaaat tactcacttg aattaggttc
    38281 aggctcattc attccaggtt ttgaagatca attagttggt cataaatcag gtgaaagtct
    38341 agatgtaaat gtaactttcc cagaagatta tcaagcagaa gatcttgctg gaaaagaagc
    38401 agtcttcaaa gtgacaatcc atgaagtaaa agcaaaagaa gtgcctgaat tagatgacga
    38461 attcgcaaaa gatgttgatg acgaagtatc aacattagac gaattgaaag aaaaatatcg
    38521 caaagaatta gtagaaacaa aagaaaaagc agcggacgat gcaaaagatg aagcagcgat
    38581 ccgtatggct gttgaaaacg cagaaatcgt tgaattgcca cacgtgatgg ttcatgatga
    38641 agtacatcgt tcaatggacg aattcttgaa caacatgcaa cgtcaaggta tctctcctga
    38701 aatgtactat caattaactg gttctacaga agaagacttg cataaacaat tcgaaggtga
    38761 agcagaaatg cgcacaagaa caaatcttgt gatcgaagca attgcagctg ctgaaaatct
    38821 tcaagcaaca gaagaagaaa tcaatgcaga aatccaagaa ttatcagaaa catacaacat
    38881 gccaatcgaa caaatcaaac gcgtgttgac agaagatatg ctggaacatg atgtgacaat
    38941 gaaaaaagca atcgaattga tcacaggtac agcgacagaa gcgaaagaat agaaatcaag
    39001 taataaatca tttctgaaga agctacttct agaaaattgt ttattcgaaa taagggactg
    39061 tgacacgatt ttgtcacagt tccttatttt ttgttaagag taaaaaatgc aaaggtatag
    39121 gtcagttgat cctttgttaa acactttttc tttttactaa actatgttat gatagtaaaa
    39181 gactgtaaag tctttgatag tttcttgcat actttttgca agttaagggg tgaagaccat
    39241 gtatgacaat ccaagtagca atgagaccgt acgctgttct ttttgcggga aaacccaaga
    39301 agaagtgaaa aagatcgtag ctggtccagg ggtctacatc tgtaatgaat gtatcgacct
    39361 atgtaaagaa atcattgatg aagaatttta tgaagaagct gtacgcgaat tcactgaagt
    39421 ccctaaacca ctagaaattc ttgaagtatt gaataactac gtgattggac aagatcgcgc
    39481 aaaacgctcg ttagctgttg ccgtatacaa tcactataaa cgtgtcaatc agcaagcaca
    39541 agaaaacaca gatgatgttg aactgcaaaa aagcaatatc tgcttgatcg gacctacagg
    39601 gtctggtaaa acatttttag cacaaacatt agcaaaaaca ttgaatgtac cttttgcaat
    39661 cgcagatgcg acaagtttga cagaagctgg ttatgtgggg gaagatgtag aaaacatttt
    39721 gcttaaattg cttcaatctg ctgattacaa tgtggaacgt gcagaaaaag ggatcatcta
    39781 tattgatgag atcgataaaa ttgcgcgtaa aagcgaaaat gtatctatta ctcgtgacgt
    39841 ttccggcgaa ggtgtccaac aagccttgct gaaaattcta gaaggaactg ttgcaagcgt
    39901 gccacctcag ggaggacgta aacatccaca tcaagaattt attcagattg atacgacgaa
    39961 catattgttc atcgtaggtg gtgcatttga tggcatcgaa acgattgtga agaatcgtat
    40021 gggcgaaaag acgattggat ttggtacaca aaaccaaaaa ctttctgaag acgaaagtgt
    40081 catgcagcat atcattcctg aagatttact aaaattcgga ttgatccctg agtttatcgg
    40141 acgattacct gttatggcag ctttagagaa attgacaaca gatgatctgg tacggatctt
    40201 gactgagccg aaaaatgcgc tagtcaaaca atatcaaaaa cttctttctc tagataatac
    40261 cgaacttgaa ttcgaacctg aagcgttgag agccattgcg aaaaaagcaa tcgaacgcaa
    40321 taccggtgct cgaggacttc gttccatcat tgaagaaatc atgatggatg tgatgtttga
    40381 tatcccttca aatgaaaaca tcgaaaaagt gattatcaca aaagaagcag ctgaattgtc
    40441 tggtgagcca acagtaatct ataaagattc agagaaaaaa gcgggttgat gaaaagaggc
    40501 tgccgtaaaa gtcattttgg cttttgcgac agccgcttgc tttattcatc accttgttaa
    40561 ggagagagac catgaaagtc catcaagctg aaattatcat aagtgcagtg gcaccaaaac
    40621 aatatccaga tacacaattg ccagaaatag ctctggcagg acgatcaaat gtagggaaat
    40681 cttcttttat caacacattg attgatcgaa agaatttagc ccgtacttcc ggtaagccag
    40741 gaaagaccca gacattgaat ttttatttga tcgagaacgc attacatttt gtcgatgttc
    40801 cgggatatgg gtatgcaaaa gtatccaaaa ctgagcgagc aaaatgggga aagatgatcg
    40861 agacatattt aacgactaga gaccagctac gtgcggtcgt ttctttagta gatttgcgtc
    40921 ataagccttc tcaagaagat atccagatgt atcaattttt gaagtattat gatatccctg
    40981 taattgttgt tgccactaag gcagataaga tccctcgtgg taaatggaac aagcatgaga
    41041 gcgaaatcaa aaaagcgttg gattttgatc gcaatgacac gtttattctc ttttcttccg
    41101 ttacaaaaca gggaaaagaa gaagcgtggg cagcaatcga acaagcaatt gaatcataaa
    41161 aagaataaaa aagacttacc agtcaaactc tttcataaaa gtttggctgg taagtctttt
    41221 tttgagctta acttactttt gtttcttgtc ttttttcttt tcattttgtg gctgtggttt
    41281 tagaaatcga tcgatctctt cttttggtgc aactgttttt tccggatcga cagcaaatga
    41341 ttcgaaaagt ttatctaaat tatctgtacg ttcaaatttc aaccccattg taaacacctc
    41401 tttgtttctt tttacttagt ctatcatgta atcgtccaaa ttggtttcta agacataaag
    41461 ttttagtttt tctttatcgt tggttataat taaattataa aaagatttta aagaggtgag
    41521 aaaatgattg gcgaagataa attattgatg actatccagc tcacagactt aaatgaagaa
    41581 gaaaaaaaac aggaaataag cgattgggct attttgacac gtctgttgat ccagccaaca
    41641 tttatccgct ctttcacaca acaagcggat ccgtatgatc ttcgaaagct tcaagaaaaa
    41701 ataatgctag catcggtaag agacgaaagt tggttagttg taggaaatga cgagaacgaa
    41761 tgtagttttc gcttggaaga taatcaatta ttgataaaaa atgtattgtc tatctctgtt
    41821 ttcaaagaaa agcaattttt gattcgagac ttcattcaga aaaaaatgat tgctcatggg
    41881 gtgttcgcct atatgcgagc ttatagcgag tttatctatc acaatacaaa acagatctct
    41941 caacggctat tgtttgagaa aaaggatgaa atcgaacatt tacctaaaat gaaacaacaa
    42001 aacggggaag tagtagtcga ttgcaaccaa tttccaggat acgatctttt ttaccaaggt
    42061 ttatgtttca cttcgtgttg ggaaatgtat tatagtcgct attatcatca aatcatccct
    42121 aaaccgattt ttctcgacat ccaacaagta gaaaaagtga aggaattaga aaacgaagtc
    42181 atatgtatcc agctttatcg agaccccttt aactggcaaa agccaaacaa tcaaatgttt
    42241 caatcctatt ttagggacca gcttgggttt gatcatcttg cttgggacaa tggtgtcggt
    42301 ttattgaaac cgccattcgt tgaatacatt tacactgacc atacgatcca atctgtccag
    42361 tatcagaatg cacaaatgca acctgttcct aaaaagaatg cttcattttt tgtaacaaaa
    42421 agttatgata ttcaacaagg agattataaa gaaaggcgtg ttcgcggaac actaaatgct
    42481 caggcgtatt ttccttgggt tgacgagaat cgctcgcgca tgatgtgcta taaagttatc
    42541 gatccgactg ttgctttgga taatggaatc gaagcttatt gttattatat tagggaatat
    42601 ctagaagtag aggtaacaga tgaaaaatat caagagtatc ttttatcctt aagaatctat
    42661 gttccttcag aatccctgtc agaattaccg ttaaaggaaa tcaaacatag actctctgat
    42721 gttaccttca aacgaataaa gaaaaagcgg cgaagtatcc aattcgatgt caagaaaggt
    42781 gagcagcatc tgcgtgtgca atttttagat taccgagaat tagaacaatt gattacttta
    42841 caaaaaatat gaggtgaaga aaatggataa acaactctat tatcgaataa tggttccggc
    42901 atcattatta tccgtctgtc tcttttttgt tcctaatttt cctattcgat cagtcatcgg
    42961 ttttttggtc atgtttggcg ggatgggact ttattattat cttcttcata aggaaagaaa
    43021 acctgcttct gtttatatga aaaaaacaag aagcgaaaat aaaaaacgat aaaacggctt
    43081 gatgatagac aaaaaaataa aaaaccag
//
LOCUS       DAZUKC010000018        43077 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000018 DAZUKC010000000
VERSION     DAZUKC010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.018"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            608..1573
                     /locus_tag="ACG80T_000874"
     CDS             608..1573
                     /locus_tag="ACG80T_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6017 domain-containing protein"
                     /protein_id="HFS4840544.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            1595..1807
                     /locus_tag="ACG80T_000875"
     CDS             1595..1807
                     /locus_tag="ACG80T_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFS4840545.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            1882..2358
                     /locus_tag="ACG80T_000876"
     CDS             1882..2358
                     /locus_tag="ACG80T_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HFS4840546.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            2355..4163
                     /locus_tag="ACG80T_000877"
     CDS             2355..4163
                     /locus_tag="ACG80T_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFS4840547.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            4240..4455
                     /locus_tag="ACG80T_000878"
     CDS             4240..4455
                     /locus_tag="ACG80T_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family mobile element protein"
                     /protein_id="HFS4840548.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            4547..5410
                     /locus_tag="ACG80T_000879"
     CDS             4547..5410
                     /locus_tag="ACG80T_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB6/TrbL-like conjugal transfer protein,
                     CD1112 family"
                     /protein_id="HFS4840549.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            5447..5983
                     /locus_tag="ACG80T_000880"
     CDS             5447..5983
                     /locus_tag="ACG80T_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFS4840550.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            5997..6419
                     /locus_tag="ACG80T_000881"
     CDS             5997..6419
                     /locus_tag="ACG80T_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HFS4840551.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            6349..8748
                     /locus_tag="ACG80T_000882"
     CDS             6349..8748
                     /locus_tag="ACG80T_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HFS4840552.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            8780..10771
                     /locus_tag="ACG80T_000883"
     CDS             8780..10771
                     /locus_tag="ACG80T_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFS4840553.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            10794..11045
                     /locus_tag="ACG80T_000884"
     CDS             10794..11045
                     /locus_tag="ACG80T_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HFS4840554.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            11035..12264
                     /locus_tag="ACG80T_000885"
     CDS             11035..12264
                     /locus_tag="ACG80T_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1107 family mobile element protein"
                     /protein_id="HFS4840555.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            12261..14342
                     /locus_tag="ACG80T_000886"
     CDS             12261..14342
                     /locus_tag="ACG80T_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFS4840556.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            14491..18411
                     /locus_tag="ACG80T_000887"
     CDS             14491..18411
                     /locus_tag="ACG80T_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodL domain-containing protein"
                     /protein_id="HFS4840557.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLPNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            18412..19356
                     /locus_tag="ACG80T_000888"
     CDS             18412..19356
                     /locus_tag="ACG80T_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840558.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            19360..19563
                     /locus_tag="ACG80T_000889"
     CDS             19360..19563
                     /locus_tag="ACG80T_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HFS4840559.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(19863..20099)
                     /locus_tag="ACG80T_000890"
     CDS             complement(19863..20099)
                     /locus_tag="ACG80T_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4840560.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(20282..20653)
                     /locus_tag="ACG80T_000891"
     CDS             complement(20282..20653)
                     /locus_tag="ACG80T_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HFS4840561.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(20650..21978)
                     /locus_tag="ACG80T_000892"
     CDS             complement(20650..21978)
                     /locus_tag="ACG80T_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFS4840562.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(21939..22268)
                     /gene="mobC"
                     /locus_tag="ACG80T_000893"
     CDS             complement(21939..22268)
                     /gene="mobC"
                     /locus_tag="ACG80T_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFS4840563.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(22526..22897)
                     /locus_tag="ACG80T_000894"
     CDS             complement(22526..22897)
                     /locus_tag="ACG80T_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4840564.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            23042..23227
                     /locus_tag="ACG80T_000895"
     CDS             23042..23227
                     /locus_tag="ACG80T_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS4840565.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            23385..23552
                     /locus_tag="ACG80T_000896"
     CDS             23385..23552
                     /locus_tag="ACG80T_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840566.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            24170..24837
                     /gene="vanR-B"
                     /locus_tag="ACG80T_000897"
                     /pseudo
     CDS             24170..24837
                     /gene="vanR-B"
                     /locus_tag="ACG80T_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
     gene            24840..26183
                     /gene="vanS-B"
                     /locus_tag="ACG80T_000898"
     CDS             24840..26183
                     /gene="vanS-B"
                     /locus_tag="ACG80T_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFS4840567.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            26354..27160
                     /locus_tag="ACG80T_000899"
     CDS             26354..27160
                     /locus_tag="ACG80T_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HFS4840568.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            27178..28005
                     /gene="vanW-B"
                     /locus_tag="ACG80T_000900"
     CDS             27178..28005
                     /gene="vanW-B"
                     /locus_tag="ACG80T_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HFS4840569.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            28002..28973
                     /locus_tag="ACG80T_000901"
     CDS             28002..28973
                     /locus_tag="ACG80T_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFS4840570.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            28966..29994
                     /gene="vanB"
                     /locus_tag="ACG80T_000902"
     CDS             28966..29994
                     /gene="vanB"
                     /locus_tag="ACG80T_000902"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFS4840571.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            30000..30608
                     /locus_tag="ACG80T_000903"
     CDS             30000..30608
                     /locus_tag="ACG80T_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFS4840572.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(30860..31864)
                     /locus_tag="ACG80T_000904"
     CDS             complement(30860..31864)
                     /locus_tag="ACG80T_000904"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4840573.1"
                     /translation="MQRGGKSRGKAAAAGSGKQKASNGERFLKKIERTGGEAVNRLTR
                     RRKVYEAIHTLRQEKGYEIASLCALSGIPRCSYYKWLHREESASEKENTLLLEEIIRL
                     YSEVKGIYGYRRMTLNVNSRLKRNYNHKRIYRLMKSVHMQAVIRRKKKHYVILEPRVT
                     AENVLNREFTANGPNEKWLTDVTEFKLINGKKAYLSAILDLHDKSIVAYALGQSNNNQ
                     LVFDTFDRAVRANPGAHPLFHSDRGYQYTNRQFKAKLDGIHATQSMSRPGRCIDNGPM
                     EGFWGILKSEMYYLRQFHTFEELKKAIDEYIDFYNTRRLQAKLKGLAPLAYRNQTLVA
                     "
     gene            complement(31747..32430)
                     /locus_tag="ACG80T_000905"
     CDS             complement(31747..32430)
                     /locus_tag="ACG80T_000905"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4840574.1"
                     /translation="MSLKGKIPPEVKIQAVEDYLAIRKGSTQIREELGIRLSTFQAWL
                     RKYQTEGPAGLHPKKHVTSYPSSLKLAAVEDYMGGGGSLDAMCRKYGISSHAVLQQWI
                     TLYNQGHREFKTRRAREETDMAEKEKLSQEQKLQAVLYCLEHQLDYRRTSEQYKITYQ
                     QIYSWVKKYQEQGEAGLLDRRGRRRPPSEYSEEEKAAAKLRQLEAENRRLQMENDFLK
                     KLNELEGRR"
     gene            32876..33307
                     /locus_tag="ACG80T_000906"
     CDS             32876..33307
                     /locus_tag="ACG80T_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS4840575.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
     gene            33314..33547
                     /locus_tag="ACG80T_000907"
     CDS             33314..33547
                     /locus_tag="ACG80T_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4840576.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            33971..34171
                     /locus_tag="ACG80T_000908"
     CDS             33971..34171
                     /locus_tag="ACG80T_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFS4840577.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            34255..35448
                     /locus_tag="ACG80T_000909"
     CDS             34255..35448
                     /locus_tag="ACG80T_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4840578.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(35627..35905)
                     /locus_tag="ACG80T_000910"
     CDS             complement(35627..35905)
                     /locus_tag="ACG80T_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840579.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFF"
     gene            complement(36023..36283)
                     /locus_tag="ACG80T_000911"
     CDS             complement(36023..36283)
                     /locus_tag="ACG80T_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840580.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(36420..36833)
                     /locus_tag="ACG80T_000912"
     CDS             complement(36420..36833)
                     /locus_tag="ACG80T_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFS4840581.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(37289..37774)
                     /locus_tag="ACG80T_000913"
     CDS             complement(37289..37774)
                     /locus_tag="ACG80T_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840582.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(37913..38128)
                     /locus_tag="ACG80T_000914"
     CDS             complement(37913..38128)
                     /locus_tag="ACG80T_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFS4840583.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            38352..39152
                     /locus_tag="ACG80T_000915"
     CDS             38352..39152
                     /locus_tag="ACG80T_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4840584.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            39171..41039
                     /locus_tag="ACG80T_000916"
     CDS             39171..41039
                     /locus_tag="ACG80T_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFS4840585.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            41032..41523
                     /locus_tag="ACG80T_000917"
     CDS             41032..41523
                     /locus_tag="ACG80T_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFS4840586.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            41511..42065
                     /locus_tag="ACG80T_000918"
     CDS             41511..42065
                     /locus_tag="ACG80T_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFS4840587.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            42083..>43077
                     /locus_tag="ACG80T_000919"
     CDS             42083..>43077
                     /locus_tag="ACG80T_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFS4840588.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT        11634 a        10312 c        11036 g        10095 t
ORIGIN      
        1 gtaaagtgga ttgatttgag atctttttat acttcaaaaa agataacacc caactttcaa
       61 aaatatgttt tggaagttgg gacaaacgag ttaatacatt ttaatacatt attttttatt
      121 caaaaatttt aggttatcgc tgaaaacccc ggaaatacgg gcatttcgca ggatatgaga
      181 actcaattta ctaccaattt actactttcg gattgagccg catatatgct gaaccatact
      241 gctaataccc gccaccccaa atacggcaca tcataaattg aatacgacac cgtaaacgcg
      301 gcgttcctct ttcccgtcat gggagaacag gacgccgctt ttttatgccc aaatagagaa
      361 aggaggcgac gccatgctat ttacttccgg ccctgaccgg gcgtttgaac ggctgatgca
      421 ggaaaagccc ggtttcgatc actacgacgg cggcggggcg gacgcgccgg aggattgcgg
      481 cacctgccga ttttaccgcc cggattggaa atatcaattc tgccagtttg cagagtgtcc
      541 ctatcagccg ggcaagctga ccgactatgc agcggtcaaa ttcacagtga aaggagcgaa
      601 caacgctatg gcagttttcc gtgtggaaaa gaacaaaggc tatacggtga tgtcaaatca
      661 ccacctgcgg aataagaacc tgaccttaaa ggccaagggc ctgctgtcgc agatgttgtc
      721 cttgccggag gattgggact ataccctaaa aggactgtcc tatatcaacc gggaaagcat
      781 cgacgcgatc aggacagcgg tatgggagct tgaaaaagct ggatatatcc ggcgtgagca
      841 gggccgtgac gcaaaaggca aaatggccga tatggtctat accatctacg aacagccggt
      901 attggaaaac ccggtattgg aaaatccaac atcggataac ccggtgttgg aaaatccgac
      961 atcggataat ccggcgtcgg aaaatccaac gcaattaaat aaagaagtac aaagaactga
     1021 cttatcaaaa aaagaaaaaa taattacaga tggacaaagt acccattcca ttcctatccc
     1081 atccccgacc cctgccccta tggagggcga ggcagcggct acgccgccgg aacggaaaag
     1141 gacaggaaag gatgaggctg tacggattta tcgggaaatc attttggaga acatcgagta
     1201 tgcctatctc attcaggaca ggagcattga ccgtgaacag cttgacgaaa tcgtagacct
     1261 gatgctggaa accgtctgca ccgcccgaaa gacaatccgt gtcgccgggg acgactaccc
     1321 tgccgagctg gtgaaatcca agtacatgaa gctgaccggc gaacatatcc gctttgtcct
     1381 tgactgtctg cgggagaaca caacgaaagt ccgcaatatc aaacaatacc tgcgggctat
     1441 gctgttcaac gcccccagca ccatttccaa ctactacacg gcccttgtcg cgcacgacat
     1501 ggcacagccc gattggggaa agcctaaatc cggcctgccg gactattcct gcggcgaggg
     1561 cgaaagcctg taacgagaaa aggaggacga ccctatgaag cagggcgcat tgatttttga
     1621 cgagcgaaca gaccgttacg acatccgctt tgatctggcc gactattacg gcggcctgca
     1681 ttgcggggag tgtttcgacg tgatgatcgg cggcaaatgg aggcccaccc gcattgaaat
     1741 ggccgaaaac tggtatcttg tcggcatccg ggccgacgac ctttccggcc tgcgggtgcg
     1801 gatttgacct atatctacct gcggcgtctg gtggttccgc cttagacgcc gcttttgact
     1861 ttctgacaag gagggatgcg attgcaggaa gaagttactc aaaagaccat tgccctgtcc
     1921 atgaaaacgg gcaagctcac ggctcaagtg ctgcaagcgg cgctgaaaaa gtacctgcaa
     1981 caccgggcca agggcaaaaa cacgctgcac cacggccagc agagcctaaa gcagcttaaa
     2041 aagcatgggg ctgatctctc caacattgag atcaccgagg ccaacattgg agcgttcaag
     2101 ccatgcgcca agaaatacgg cgtggatttt accttacgaa aagataagac cacccagccg
     2161 ccccactacg ttgtgatttt caaggccaag gatgcagata accttgatct ggcgtttcgg
     2221 gagtttaccg ccaagacgct ttccaaggag cagcggccct ctatccgcaa ggttttgtcc
     2281 gctgtgaagc aaaaggcggc ggcccagacc aagcagcggg ccaaggagaa aatcaaggaa
     2341 agggggctgg aactgtgaag cctgacgtaa agaaattgct gatcctgaac ctcccgtatc
     2401 tgatttttgt gtacctgttt gcgaaatgcg gggaggcgta ccggctggcc gctggtgctg
     2461 acctgtcggg gaagctcctg cattttgcgg atggttttgc ggcggctttt gccaacccgc
     2521 tgccaagcct gcacctgttt gacctgtgta tcggtatcgc cggtgccgtg atcgtgcggc
     2581 tggtggtgta ctgcaagagc aagaacgtga agaaataccg caaaggcatg gagtatggca
     2641 gcgcccgctg ggggacggcc aaagacatag cgccttacat cgaccccaag tttgaaaata
     2701 atatcctgct cacccaaacg gaacggctta ccatgaccgg caggcccaaa gaccccaaga
     2761 cagcgcggaa taaaaacgtg ctggtgatcg gtggcagcgg ttcagggaaa acgagatttt
     2821 tcgtgaaacc caacctgatg caatgctttc cgaccacgga ctatccgact tcgtttgtgg
     2881 tgacagatcc gaaaggcact ttggttctgg aaaccggcag gatgttccag caggcgggct
     2941 accgcattaa aatcctgaat accattaact tttccaagtc catgaagtac aacccctttg
     3001 tctacatcca ttcggaaaag gacgtgctaa agctggtaaa taccctgatt tccaacacca
     3061 agggcgaggg ggaaaagtca gcggaggatt tttgggtgaa gtcggaacgg ttgttttatt
     3121 cggccctgat cggctatatc tggtacgagg ccccagcgga ggaaatgaac ttctcaacgc
     3181 tgctggaaat gataaacgcc agtgaagccc gcgaggacga cccggagttt caaagccccg
     3241 tcgatctcat gtttgagcgt ttggagcaga aagaccccga ccattttgcg gtgcgccagt
     3301 acaagaaatt cctgctgtcc gcaggcaaga cccgttcttc catcctgatt tcatgcggtg
     3361 ccagacttgc gccctttgac atccgggagg tacgcgagct gatggaggac gacgaaatgg
     3421 agctggacac catcggggac gaaaagacgg tgctgtatct cattatgagc gatacggaca
     3481 cgacctttaa tttcattctg gctatgctcc aaagccagct aatcaacctg ctttgtgacc
     3541 gggctgatga tgtgtacggc gggcggctgc ctgtccatgt acgcctgatt ttggatgaat
     3601 ttgccaacat tggccaaata cccaatttcg ataaactcat tgccaccatt cgaagccgtg
     3661 aaatatcggc ctctattatt ttgcagagcc agtcgcagct aaaagcgatt tacaaggacg
     3721 ccgccgagat catttcggat aactgcgata gtgtgctttt ccttagtggg cgcggcaaaa
     3781 atgccaagga gatttccgat gcgctgggga aagaaacgat tgacagtttc aacaccagcg
     3841 agaaccgggg aagccaaacg tcccacggat tgaactatca aaaattagga aaggagttga
     3901 tgtcccaaga cgaaattgcc gtgatggacg gcggcaagtg tatcttgcag ctacggggtg
     3961 tgcgcccgtt tttatcggag aaattcgata ttaccaagca tccccggtac aaataccttg
     4021 ccgacgccga caagaagaat acctttgacg tggacagatt tttgactatc acgcgccgga
     4081 aacggcagca agtggtaacg caggatgaag tattcgacct gtatgaaatc gacctgtcgg
     4141 acgaggacgc cgccgctgaa taaacagcgg cttttttcat tccaacaaca aaatcaaaaa
     4201 atcattatga gccggaaacc ggcagaaaga ggtaaattta tggcattttt taattccgca
     4261 gttactactt tgcagactat cgtgatcgga ctgggcggcg ctctgtgcgt atggggcggc
     4321 atcaatctgt tggagggata cgggcaggac aatccggggg caaaatcgca gggtgtcaag
     4381 cagcttgtcg ctggcggcgg cgtggccctg atcggtgtca ctctggttcc cctgctgtcc
     4441 gggctgctgg gctgatcctg gcctttgtcc ctgtccaaac ccttaactaa tcgcctccgc
     4501 gtcctcccgg ccagaccggg agggcgcggg aaaggaggtg ctacccgtga ttagtgaaat
     4561 cattgaagaa tggataaaag gcatcctcat ggacggtatc acgggcaacc tgtccgggct
     4621 gttcgatacg gtaaatgcca aagtcggaga aatcgccgcc gacgtgggac gaaccccgca
     4681 ggcatggaac agcggtattt tcaatatgct gcacagcctg tcggaaaccg tgatcgtccc
     4741 catagccggg gccatactcg cccttgtcat gtgctatgag cttatccaga tgataacgga
     4801 aaagaacaat atgcacgact ttgacagctc catgttcttc cgctggatat tcaaatccgc
     4861 gtttgcaatc ctgatcgtaa cgaacacatg gaacatcgtc atgggcgtat tcgacgccac
     4921 ccaaagcgtc gtcaatcaaa gcgccggtgt gattatcggg cagacatcca ttgattttaa
     4981 caccctgata ccagaccttg aaagccaatt ggaggctatg agcgtgggcg ctcttttggg
     5041 gctgtggttc caaacccttg ttgtgggcct caccatgaat atcctttcca tctgcatttt
     5101 ccttgtgacc tatggccgca tggtggaaat ctatgttgtc acgtcgctgg ggcctatccc
     5161 ccttgccaca atgggcaacg cggaatggcg cggcatgggc caagggtatc tgaaatccct
     5221 gctggcgctg ggcttccaag cctttttaat catggttgtg gtgggtatct acgcggtgct
     5281 gatacagaat atcggttccg cttccgacat atccggcgca atctggggct gtatgggcta
     5341 tacggtgctg ctgtgcttct gcctgttcaa gaccggcagt atatcaaagg ccgtattcgg
     5401 ggcgcattga tcccaaaatc caagcataca caggaggaac ttgtttattt caacgtacaa
     5461 catcatttac gctgacccgc cgtggcggta tgactgcaaa aacgtacagg gcgcagcgga
     5521 aaatcactat tccaccatga gcattgacga gctgtgcgcc ctgccggtgg aacggctggc
     5581 gtccaaagac tgcctgctgt ttttgtgggc cacattcccc atgctgccgg aagctctgcg
     5641 gttaatcaaa gcgtggggct tttcattcaa gaccgtggct tttgtatggc tgaaacagaa
     5701 ccgtaaaagc cttacatggt tttacgggct gggccgctgg acaaggggaa acgcggaaat
     5761 ctgcctgctg gcaacacacg gcaagccgaa gcgtcgttct gcctctgtcc atcagtttat
     5821 tatcagcccg attgaacaac acagcaaaaa gccggatgtg acccgtgaaa aaatcgtaga
     5881 gctggcgggc gatttgcccc gcgtggagct gtttgccaga cagaaaaccc ccggctggga
     5941 cgtgtggggg aacgaggtgg aatgtgacct tatcctgacg tgaaaggagg tgccaaatgg
     6001 cttatgtaac cgtccccaag gacttaaccc atgtgaaaag caaggttgtg tttgggctta
     6061 ccaaacggca actgatctgt ttttccgggg cgctggtgat cggcgtcccc ttatattttc
     6121 tcacccgtga ctatcttacc aacagcgcgg cggccctgct catggtattt gccatgctgc
     6181 cgggcttttt gcttgccatg tacgagcgtc acggccagcc ccttgaagtg gtgattatgc
     6241 agatcatcca gtgctgcttc atccagccaa aggagcgtcc ctatcagacc gacaacacct
     6301 acaccgccct tgtgcggcaa taccagatgg aacaggaggt aaaggccatt gtccaaaaag
     6361 gaaatcccaa gagaaaccga aaaaacaaag ctgacccgcg cccagcgaaa agaaattgac
     6421 gcggtaatcc gtaaatataa gggcgacggc aggccccaca cggcgcagca gagcatcccc
     6481 tacgaggtca tgtacccgga cggcgtgtgc cgggtatcgc ccggcgtctt ttccaagtgc
     6541 attgagtttg cggacatcag ctatcagctt gcccagccgg acacgcagac ggctatcttt
     6601 gaaaaactgt gcgacctgta taactatgtg gacgcctcca ttcatatcca gttttctttc
     6661 ctcaatcgga aagttgaccc ggtgcagtac gccaagagct ttgagatcgc gccgcagggg
     6721 gacgacttcg acgacatccg ggccgagtac accggcattc tgcaaaagca gcttgcaaac
     6781 ggcaacaacg gcatggtaaa gaccaaatac ctgacattca ccatcgaggc cgaaagcgtg
     6841 aaagcggcgc gagccagatt gaagcgcatc ggctttgacc tgttaggcta tttcaagagc
     6901 atgggggccg tagcccatgt gatggacggc tgggaacggc tgaacctgct gcatggcgtg
     6961 taccacccgg acggcgagat attcaatttt gactggaagt ggctggcccc gtccggcctc
     7021 tccaccaagg attttattgc cccgtcctcc ctgtgcttcg gcaatgcaaa gacattcggc
     7081 atgggcggca aatacggcgc ggtaagtttc ctgcaaatcc tgtccccgga gctgtcggac
     7141 gatatgctgg ccgacttcct caatacggaa agcggtgttt tagtcaatct ccatgtgcag
     7201 gccattgaac agaccaaggc gatcaagacc atcaagcgca aaattaccga ccttgacgct
     7261 atgaagattg cggagcagaa aaaggccgtc cgcagcggtt acgatatgga cattcttccc
     7321 agcgaccttg ccacctacgg cgaggacgcc aagaagctgc tgacaaagtt gcagacccgc
     7381 aatgaacggc tgttccagtt gacgttcctt gtcctcaacg tggccgacac caagcaaaag
     7441 ctgaataacg acgtgttcca agccgcaggc gtggcccaaa agcataactg tccccttgtc
     7501 cggctggact accagcagga acagggcctt gcaagcagcc tgccgctggg cgtcaatcag
     7561 atcaagatac agcgcagcct tacgacttcc agcgtggccg tgttcgtccc ctttgtgacg
     7621 caggagcttt ttcagggcgg cgcggccatg tattacggca tcaacgccaa gtcccgcaac
     7681 atgattatgc tggacaggaa gcaggcccgt tgccccaatg ccttgaagct cggcaccccc
     7741 ggcagcggca aatccatgag ctgtaaatcc gagatcgtca gtgtgttctt aacgacgcct
     7801 gacgatattt ttatttcaga cccggaggcc gaatattacc cccttgtcaa acggctccac
     7861 ggacaggtaa tccggctgtc gcctaccagc aaggactttg tgaacccgct ggacatcaac
     7921 ctgaattatt cggaggacga taacccgctg gccctgaaat ccgactttgt gctgtcgttc
     7981 tgtgagcttg tcatgggcgg caagaacggg ctggaagcca ttgaaaagac cgtgatagac
     8041 cgggctgtgc gcgtgatcta ccggccctat ctggcagacc cccgcccgga gaatatgccg
     8101 atcctgtccg acctccacaa agcgttgtta gatcagcatg tcccggaggc tgaccgggtg
     8161 gcgcaggccc ttgacctgta tgtgtccggc tccctgaacg tatttaacca ccgtaccaac
     8221 gtggacatcg gcaaccggct tgtgtccttt gacattaagg agctgggcaa gcagctcaaa
     8281 aagctgggga tgctcattgt ccaagaccag atatgggggc gcgtcacggc caaccgaagc
     8341 caaggaaagg ccacatggta ttttgccgac gagttccatt tgcttttgaa agaggaacag
     8401 acggcggcct attctgcgga gatttggaag cggtttagga aatggggcgg cattccaaca
     8461 ggtgccaccc agaacgtcaa agacctgctt tcctccccgg aaattgaaaa tatccttgaa
     8521 aacagcgact tcattacgct gctcaatcag gcgtccgggg atagaaaaat ccttgcggaa
     8581 cgcttgaatt tgtccaccga acagcagaag tacatcgaca attccgaacc cggcgagggc
     8641 cttttgatct ttgaaaacgt ggtgctgccc tttaccaacc ccatcccgca caacacccag
     8701 ctttacaaga ttatgacgac ccgcttaaac gaggtggcgg gagtatgacc cggcgacgcc
     8761 acaaaggagg tgaaccacca tgaaagagct gaaagccaag gcgaaagtga cgcaggccat
     8821 gacccgcgac gggctgacgc tggaaaatca ggccaccggc gaggtggaga acatttccag
     8881 ccgggaggcc gaacaggatt tgtccgcgcc ggggccgggc ggcaccgccg ataagctgct
     8941 ggaacgggcc gacgccatcc atgacggcca taaggccaag aaagccgcga agcaggccgg
     9001 ggctgtggcc gccgaggggg acgctgccct gcaccggcca tccgcccggc tccaactgtc
     9061 ggaggaagaa cgggccgacc cggatttaca gccctatatc cgaaaatcgg acaaacgggc
     9121 cgataagctg gataaggccc gcgccgccat ccccaagaag cgcgtcccgg tcaaggaaac
     9181 cgtctttgac gaggcaagcg gcaaagcaaa gaccgtcctg cactttgaca aacaggacaa
     9241 ggggcctccc tccctgaaac ccaatccggc aggccgtcct gtttccgagg tttttctatt
     9301 cggccacggg aaaatccatg aagtcgagca tgaaaacgtg ggtgtggagg gcggccataa
     9361 gggggaggaa cttgccgagc ggcaggccgg aaaagccatc cgcagcacca tccggcacca
     9421 caggctgaaa ccctactggg acgcggaaaa ggccgagcgc aggctattgt ctgccaacgc
     9481 ggagtatttc taccagaaat ctttgcggga taacccggag atcgcacagg cggccagcaa
     9541 ccccatttcc cggttctggc agaaacagca gatcaagcgc cagtacgcca aggccgcccg
     9601 gacagcgggc cagaccgcag gcgcggcggc acagggcgcg gccaagacag ccggggcctc
     9661 cacgaaagcg gccaaggccg cagccaaaga aagtgaacgg gcggcctcct ttgtggcccg
     9721 ccactggaaa ggggcgctgg ccgtccttgt gttggggctt ttgatgctgc ttatcatcgg
     9781 cggcttacag tcctgcaccg ccatgttcgg cagcgccgga agcggcatca cggcgtcctc
     9841 ttacttttcc gaggatagcg atatgttggc cgccgaggac gcctacgcgg agctggaagc
     9901 cgagctgcaa gactatctgg acaactacga ggccacccat gactatgacg aataccattt
     9961 tgaccttgac gagatcagcc atgaccccta tgtgctgatt tccattctgt cggcgctcca
    10021 tgacgggata tttaccatcg gggaggtaca gggtgatttg tccatgctct ttgacaagca
    10081 atatatcctg acagaaaacg tcgtggtaga aacccgttac cgcaccgaaa ccgatacatg
    10141 gacggacgcg gagggcaaca cccatacaga cacctaccaa gtgccgtatg actattacat
    10201 ctgctacgtc acgctggaaa acttcgacct gtcccatgtg ccagtctata tcatggacga
    10261 ggaaacgcta agtttctatt ccctgtatat gtccacgcta ggaaaccggc ctgatctgtt
    10321 ttccggcagc cagtacccca acgcctccac cctgacgccg ccgacctact acgacgtacc
    10381 cccggaggct ttggaggatg ccacctttgc cgccatgctc aaagaggcgg aaaaatatat
    10441 cggttatccc tatgtgtggg gcggcagctc tcccagcacc agctttgatt gcagcggctt
    10501 catttcgtgg gtaatcaatc atagtggatg gaacgtggga cggctggggg cgcagggcct
    10561 atataacatc tgcacccctg tttccagttc gcaggccaag cccggcgacc ttgtattttt
    10621 caagggaacc tacgacaccc ccggcgtgtc ccacgtcggc ctgtatgtgg gcaacaatgt
    10681 gatgctgcat tgcgggaacc cgatttccta cacgaacctt acttcaaact actggcaatc
    10741 ccatttttac agttacgggc gtttacctta acgccagaaa ggagctttca attatggcaa
    10801 ccaaacttga ccgcatcgaa aaggacatcc agaaaaccaa gtctaagatc gcggagtttc
    10861 agaaacagct tcgggagctg gaaacgcaga aaaccgaaca ggagaatttg cagatcatcc
    10921 agcttgtgcg cggcatgaac atgaagccgg aggaatttgc cgcgttctta cgcagcggcg
    10981 caatgcaggc cgcccctgcc gcaacccctt accatgaaca ggaggacaat gcccatgaag
    11041 aataagacca gacggatttc ccgtattctc cccgcacttc tggccgtgat gctctgcgtc
    11101 acggcctttc ccgttaccgc ccttgcaggc ggcaatgacc cggcaccggc cccgctgccg
    11161 gaggctacaa ccgaacccgc tacgggcggt ttggagcctg aaacagacga aaccggcacc
    11221 gccacggacg ggacagaaac cgaggccgaa aaagataccg gccctctgac cggcaaggac
    11281 atcaccgacc tgttttccgc gctgttcggt tccaaggtga gcattgccgc cacggacgac
    11341 ggcatccaga tcaccaccgg caccgacaaa aaagaacctg aacagaccgg caccgtcacc
    11401 accaacggcg gcaggctgaa tgtccgcacc ggcgcggggc ttgacaagac cgcgttcacc
    11461 cagcttccca atgggacgac ggttgaggta gtcggcacag acggcgactg gattaaaatt
    11521 ctcttgccgg agcgcatcgg ctatgtccat tccgactata tgaccgtcag cgaaaaggaa
    11581 gtagcggcta ccggcgaggg cggtttttcc ctctccattg acccggagga aattgcttct
    11641 ctgctggaac tgttcaatgg cggcggggcc agcgccgccc tgtccccgga cgggaacctg
    11701 tcactgattg acgacatcgg cagcgccgcg aaaaatggca agcagtttat caccgtggaa
    11761 accaaaaacg gcaatgtgtt ttatctgatt attgaccggg acgacgaggg aaaagaaact
    11821 gtccatttct taaatcaggt ggatgaagcc gatctgctga cgctgatgga ggacggggaa
    11881 accgtcaccc ccgctgctgt ctgctcctgt accacgaaat gcaaggccgg ggctgtgaac
    11941 acgaattgcc cggtatgtaa aaccaacctg accgagtgca gcggcccgga gccgcaggaa
    12001 ccccagccgg aggaacccga agccccggag gaagaaccca agggcggcgc gggcggcctg
    12061 atcgtgttcc tgctggtggc gctggcgggc ggcggtgcgg ccctttacta tttcaagttc
    12121 aaaaagccca aggctgaaac caccggcagc gacgatttgg gcgagtatga ttttggggag
    12181 gacgaggatt tggaggacga ggaacccgaa accgagtttg acccggaagc tgattttgtg
    12241 agtgaacagg aggacgagga atgacatata aacttgtaat cgcggaaaaa cccagcgtgg
    12301 cccgcagcat tgcgggcgtg atcggggcca cggagaaaca cgacggctat ttggagggga
    12361 acggctatct ggtgagctgg tgcatcggac atctggtgtc ctttgccgac gccggacgct
    12421 atgacgagcg tttcaaaaaa tggcgctatg aggatttgcc cattctcccg gaaacatggc
    12481 agtacatcgt ccccgacgac aagaaacagc agtttgacgt tctctgttcg ctgatgcagc
    12541 gccccgacgt gaccggcctt gtgtgcgcca ccgacgccgg gcgcgagggg gaattgattt
    12601 tccgtttcgt ctaccagatg gcgggatgcc aaaagccctt tgagcgcctt tggatttcct
    12661 ctatggagga cagtgctatt aaggacggct ttgcccacct gaaaccgggc ggcgactatg
    12721 atccgctgta tcaatcggcc ctgtgccggg ccaaggccga ctggctgatc ggcattaacg
    12781 ccacccggct tttttccgtc ctgtaccaca agaacctgac tgtgggccgc gtccagacgc
    12841 cgaccttaaa aatgctggtg gacagggacg ccaagattac cgatttcaag aaagagaaat
    12901 atcacatcgt ccatatcacg gcgggcggcg cggacgcggt aagcagccgc ttttccgacg
    12961 ctgccgaggc aaacaccgtc aaggcggctt gtgcaaaagc gcaggccgtt tgcgtttccg
    13021 tgacgcggga gaaaaagacg gaacagccgc ccaagctcta tgaccttacc accttgcagc
    13081 gggaggctaa ccggcttttc ggctataccg ccaagcagac ccttgactat gcccaggcac
    13141 tgtatctaaa gaagctgctg acctatccga gaacggatag tcaatatctt accgacgata
    13201 tgctgcccgc cgtggaaagc cttgtgtccg gcctgtggga gctggtgccg tttgccaagg
    13261 gcctgaacct ttccccgcag tttgaccgca tcctgaacag caaaaaggtg tccgaccacc
    13321 acgccattat ccccaccgct gaatttgtga agcagggctt tggcactctg gccgagagtg
    13381 aaaaaaagct gctgtccctg atctgctgca agctcttatg tgccgtggcc gggccgcata
    13441 tctatgaggc cgtcaccgcc acatttacct gtgccgggca ggagtttacc gccaagggca
    13501 agaccgtcct ttcccccggc tggaaagaga ttgaacgccg gttccgttct tccttaaaga
    13561 ccgacgccga cgaggacgcc gaggcggtgc gggagctgcc ggagctttgc gagggccaga
    13621 cctttgcaga cgtggccgcc tctgtcaccg agcatttcac cacgccgccc aagccctaca
    13681 ccgaagatac gcttttgtcc gctatggaac gggccggggc cgaggatatg ccggaggatg
    13741 ccgagcgcaa gggattaggc acaccggcca cccgcgccgc cattctggaa aagctggtgc
    13801 agatgggctt tgtccagaga aaaggcaagc agctcattcc caccaaggac ggcatcaatc
    13861 tggctgtggt gctgccggag gcgcttactt ctccccagct tactgccgaa tgggaaaacc
    13921 ggctgaccga gatcgccaag gggcaggccg atcctgacga atttatggac ggcatcgaga
    13981 cgcaggcgcg ggagctggtg cagacctatt cctgtatcag tgaggaaaag cagaaattgt
    14041 ttcaggccga gcgtgttcct atcgggacgt gtccccgctg tggtgaggct gtctatgagg
    14101 gcaagaaaaa ctactactgc ggaaaccggg cctgccagtt cgtcatgtgg aagaatgacc
    14161 gattttttga ggaacgcaag aaagcgttca ccccgaagat cgccgctgcg ctgctcaaaa
    14221 ccggcaaagt caaggtaaag ggcctctatt ccgtaaagac cggcaaaacc tacgacggga
    14281 cggtgctttt ggctgatacc gggggcaagt acgtcaacta ccgcattgaa cacaggagct
    14341 aaaggacagt aacacagcaa gcatagggag tgtggctaca ctcccggtaa aatcccccgg
    14401 ccgcgtccct gccgggccgg ggatttttgc ttgttgggga agtccccaac cccctatttt
    14461 ttgaaacaga acagagaaag gagtgaccgc gtgaacgaag tattttctat ccaagtccat
    14521 aaccggcagt ttttggaaca gggcaaacaa ggcgtctggc tggatttgcc caccacaacc
    14581 gaaacgctgc aagccgccct gcgggatgtt ggcattactg ccgacaatcc gcaggatttt
    14641 tttatcaacg ggtattcctg cccggaggac agacaccttg ccattcccta tgacatggtg
    14701 ctggccgccg acgtggacga gctgaatttc cttgccgccc ggctgggcca gcttgacgcc
    14761 gccgagatcg cagagctgaa cgccgccctg caaaatcccc gtggcggctt tgagaatatc
    14821 ggccagatca tcgactacac cgaaaatgtg gattactatg tgcatcttcc cgacgttagg
    14881 ggggatggac agttgggcga ctactacctg aaccgttccg gcatggtgga tatgccggag
    14941 gaatggaaag ccgggattga tacggcgcag tttggacgcc acattgccca gcaggaacaa
    15001 ggcgttttta cccagtacgg ctatctggtg agaagcggcg acgaatggca gcgcgtccat
    15061 gagggccagc cggtgccgga ggaataccgg gtgatgcact acccccagcc ggaagctgac
    15121 cgggaggaac cgcaggccca gcagccggag gcatccgaaa gccccgacaa agcgccgccc
    15181 ccggttatcc cgatcatgct ggacggcatg aacagcaaag aacgcatgaa agtgattact
    15241 gaccgtctgg aaaccggcgt acaggagctt tttgaaagta gccgctatat ggcttactta
    15301 tccgctatgt ccaagatgca cgattacagt ctgaacaata cgctgctgat cgccatgcag
    15361 ggcggttcga ttgtggcggg ctataacagg tggcaggatg aatttgaccg ccatgtgaag
    15421 cggggcgaga aaggcattaa gatactggcc ccggccccgt tcaaggttaa gcagcagatg
    15481 gagaaaatcg acccggccac ccagcagccc gtgatcggcg cggacggcaa gcccgtgacc
    15541 gaagaacagg aagtgacggt tccggccttt aaggtggtga cagtctttga catcagccag
    15601 accgagggca aggattttcc catgccctat atcagtgaac ttaccggcga cgtgcagcag
    15661 tatcaggatt tctttgccgc gctggaacgg acatcaccct atcccataag cattgagccg
    15721 atttcagacg gggccaaggg ccgctgcttc tacgcaaaac agcgcattgc catcaacgag
    15781 ggcatgagcg aattgcagac cctaaagacc gccatccatg agattgccca tgccaagctc
    15841 cacgccattg accgggaggc ccccgccgag gaacaggcgg cccggccaga ccgcagcacc
    15901 cgcgaggtac aggccgaaag cgtggcctat accgtctgcc agtattacgg gctggacacg
    15961 tcggactatt cttttggcta tgtggccgat tggagcagcg gcagggagtt atccgagctg
    16021 aaagcctccc ttgaaaccat ccgggccacg gccagcgaat tgataaccga gatcgacggc
    16081 cattttgccc agcttcagca ggagcgcgag gcccggcagg aacaggaggc cgtcacggtt
    16141 gacgttaccc cggaggcagc ggccccggcg caggccgagg aagaacaggc cccggccctt
    16201 gatccggcag ccgagccgct tgtgactgtg ctatggagcg aaagccccca gctaaaggac
    16261 ggccagcaaa tgccgttgca tgaggccgac gcgctgtttg cccgtctgga tgcagcatat
    16321 ccggcagagg caggctatga caaaaccgcg ttccgcattg actgtatctt tcagggcgag
    16381 ccggacagct acgaggggcg gcaggatttt ggagataggg acggttccct gatcgaacat
    16441 atccagagct accaccaatt ttacgcgcag gacgaaaact ggaaaaagca tgtaatcgct
    16501 acaaccggca tagagggctg ggagcaggac aaggcccatc gggatatgct gttaggcgag
    16561 tttgtcccct atatgaagct gcattgcagc cttgcccaaa tggaacagac ggcgcagggc
    16621 cgcttacagt ccggggaaac cctgacgccg gaggaaacgg cctatttggg ggcgcttctc
    16681 tcttatgtgc aggagtgccg ccccatgctc aatcagggcc agtatcagtt gccggagccg
    16741 ccgaaactgg ctgacttcga ccagagcttg caggactaca aagcacaggt acaggccgag
    16801 atcgcgcagg aggccgccgc agccggtatg accgtggagg aatacgcggc ggctggctat
    16861 gaagcacccc aacaggacac cttttccatc taccagcttc gaggcgggga ggaaacccgc
    16921 gactatcggt ttgagccata cgagcgttta caggccgcag ggctggccgt ggacagtgcc
    16981 aactatgacc ttgtttatac ggcaccgctg gataaaagca cgtctttgga ggatatttac
    17041 cgcacattca acatcgacca cccggcagat tttaggggac acagcctttc cgtttccgac
    17101 gtggtggtgc tgcaccagaa tggacaggac accgcccact atgtagacag cttcggctat
    17161 atggaagtgc cggagttttt gcgggaaaat cccctgcgta ccgccgaact gtctacggag
    17221 cagaacgaaa acatgattga cggcattatc aacaatacgc cgcccgaacc gcagccggag
    17281 gaacccgaac agctatcttt tgaggatgtg gcagttgctt ccggccagac acaggaggaa
    17341 cccccggagg ctgcgccccc gcaaacggct gtccgctatt atcccatcaa tgagggagcc
    17401 gcccgccgcg ccaaggaagc catgagcttt tctgactacc ggcccggcag cgccacggcg
    17461 gaataccggc actatgtcga tcaggccgtg gaaattgcgg agcgccagaa aaagcgtgtg
    17521 gagccagagt atcacgaaaa gatagacagc ctccttgaca cctacgcccg caagctggcc
    17581 gagaacatga ataagggcta tgagattacc gcccgcgtcc cctctgtcct gattgccggg
    17641 ccgtccaatt tcccggtacg ggcgaaagaa aagcagaacg ccgccagtga ccgcaacatg
    17701 gaggaatttc agtacattca gggcttgctt gacaaaatcc gcagcaccgg catgggtggt
    17761 atcagcgccg acgatccgca ggccgtttcc aagctggaaa agaaactgga aaagctggaa
    17821 gcgtcgcagg agttgatgaa ggccgtcaat gcctactacc gcaagcatgg gacgctggac
    17881 ggatgccccc atctgaccga aagaggcatt gaaaacctga aagcggatat ggcgtcgggc
    17941 tggcactatg agaaaaaacc gttccagtcc tggcagctcc ccaacaataa cgccgagata
    18001 cgccggttaa agggccgcat tgaggaattg acccgccaaa aggaggcggc ctatgtggga
    18061 tgggagtttg acggcggcac tgtggagatc aaccgggtgg ccaaccgcct gcaaatcttc
    18121 tttgagggga agccggatgc tgctgtacgc gacgagctga aaagcaacgg tttccgctgg
    18181 tcgcccaagg ccggggcatg gcagcggcag ttaaacgaca ccacaatccg cgtcgctgac
    18241 cgtatcaagt gcatccagcc cctttccggc gaaaagccca gcgccttaca gcttgcggcc
    18301 cgcagggaga aagaaaagcc ctctatccgg gcgcagctca acgcggccaa gaccgaacag
    18361 gccaagcagc caccggcgcg ggaaaaatca aagggattgg aggtagagta aatgcagaag
    18421 tttgaaaatg tggacgttct ggccgcgctg gaacagatca tgcggcagaa caccgcgttt
    18481 tatcaaagtg actttggcat tgacaaagac attatcaggc gggatgccgc cagcgatcag
    18541 gccgcagaca agaccctgct ctggatgtcc cggccctccg ggacgtactg tttccgggaa
    18601 cgtgatgttt tcctgaaaga cacccgacag tacaacacat ggaaatttta cggggagcag
    18661 acccgtgaca agatactggc ctacgcggtg gagctgaccg gcacacaggg cggcactatc
    18721 cggggcaatc tctatgagct ggactatccg cagcatttcc ggcacgtcat tgaaaccgcc
    18781 cagccggtga gtgtgaaccg gctcttttat gagcatggca cccgtgacat cccggagagc
    18841 cagcgttttg acggaagccc tgaccgggta ttgggaaact ttttgtacta tgaggcccag
    18901 ccccatgacc cggctgtgct gcaggaggcg ctacggacgg agcagcacag ccgggggcgg
    18961 gccgtccccg gcgatttcaa agtccacgtc accgctttgc atgacagccg gattgaaacc
    19021 gaggcccggc gtattgtgga cagcctgaaa gatctgtccg cgcctaacag ccccaataaa
    19081 acgcatttca tggtggagat ctccccgcac tttgcggcga ttgcttccac caaggataat
    19141 gaccggctct ttgccatgct gccctataaa tccctgtgtt ttaccgggat gaaaggcagg
    19201 catggcgtgt atgccgtgat taaccgggac gagcgccggg atgtgagtat tcgcaggccc
    19261 cggccctcta tccgcaagca gctatccgac agcaaacagg ccgccagccc aaaaaaggcg
    19321 gcggccagaa ccaaaaagaa tgaattggag gtataaggaa tgatgcactt taccgtggag
    19381 gaagaaaacc tgatctgtat gtaccacaat gcagaccggc gcagaacgat aaacaggctc
    19441 accgccgtca tgccggttat ggatggggat atgcgggcgc aggcccagca gaccctttcc
    19501 aagctggaac gcatgacgga tgccgactat gacggccaga agttccactt cacagacgaa
    19561 taacgaacag cagacgccgg gcgcggcggc catgtggtcg cttgcgtccg gcgtcttttc
    19621 tttttgccta ttcctacgca tttagaaccc ggtattgtcg gggataggat gaagtattcg
    19681 cttgcccctg caaacatgcg ggagaagcct tgcagcgggc ggttttcaat cccttaaatg
    19741 cgtaggttgc agctataatt agacccctga aacagatata gccgcgcagc gggtgtatct
    19801 gtttcagggg tgtgcaggtg atagccgcaa agcggcgtaa gagggtgcag ccctcttgcc
    19861 gtctatctct gttccagcgg gaagctcggc aatgcttcca gcagtttcag ggaaatgcgc
    19921 tgccgtatat cttcgtcaat gactttcttc tttttgccgt ttgcctgtct gatctccacc
    19981 gtggaaagct cgttgatgta gtcagcataa tgggccaaca ctttttccgt ggcccacctc
    20041 tcaccggcaa cggcggcttt gatcgtttca tagtccagca gcttttcttg tccgtccatc
    20101 ctgatacccc cgtcagactg attttttcta tgataccata ttcggaagca tggaaccaga
    20161 gaaaacggcg cttttctgct ggtaaattct acgcatttag aacagcactt cggcggggat
    20221 aggataaaac acccacatac cccgctgacc atgccggaaa agccttgaaa tataagggct
    20281 tttacagctc taagtgcgta ggtttccagt atcttttccg ctggcgctcg gcctctttgc
    20341 ggcggcgcat ccgtttggag caggccgcgc agtatttggc ccggttagac ttcggcgtga
    20401 acgtggcccc gcactcacag cagcgtttca cttcgtcccg gtctttggtg atctcggcat
    20461 acagggccgc atccagcggg aggacagcgg ccttaaacca gcggcacaga agcgaatagg
    20521 aaatgctctg tacgcaaata cattcttcgc cgtcgtccag caacaggcag ttgccgtcac
    20581 aatagttgca gcactcatgc gtcagcttcc gggccttgcg atattgaggg taattcattc
    20641 gtggaatact catagctctt gcgtccattc ctttcccggc gtcatacgca aaatgccgtc
    20701 cacattctgc ttgatgatgc cgtattcctg catctgcttt ttcgcttcgc cgtacttctc
    20761 atacagccgt tccttttcct cggccaatcg cttgtactcg gctttcaggg cgtacagatc
    20821 ggggagcttt ttcacaccct gtccctttag ggcctttgcc gcagcttcat acaaaatcaa
    20881 cgtgctttca tgctcacgcc ggaattttgc cttatccgct gcattgcggt attccatagc
    20941 cacgggccag agctgcttgt aggtagaaat attctttatc agcaccgcca tatccgcaag
    21001 ccggtgttcc atagctttga gggtagcggc ggcctcgtcg ctggcggcgg tgatctcggc
    21061 cactttgctt tccaaatccg ggtaggtgtc tatcccatgt tcagtcagga aattcaaagt
    21121 ctttgcggcc tgcttcaaat tgtggagctt cgcccacctc tcatagcctg ccgactgctg
    21181 ggccttaatg ttgttttcca gatcaatccg caaagaaatc cccttgcgct ctttgggcgc
    21241 tctggtgata acgcggcctt tgatacgctc tttgatggct tcctcggtgt aggcttcccc
    21301 caatgtcttg cagcgggtaa agcgttcctg tccgggagcg cggaaagaaa taaactttcc
    21361 cggcttgatc tcatagccct gcgcctgcat gagccgcagg aaatcatcaa aatcttttgc
    21421 ctgcgggata gcggcgtcaa tggctgcttt gagcttcgct ttccagctcg tccctttccg
    21481 ctgggcgtcc cactcggcat agctcttgcc cttactctgg ccgggcttca ccacggacaa
    21541 tccgttttcc ttgcacagcc tgtcgctggt gcgccggata taggcatagc tctgtttgtt
    21601 ggaaatgtac ttgcgctgat ttaccatatc cacggcacag aaaataatgt gattgtggac
    21661 gtggccctta tcaatatggg tagtcagcac atagggatat ttcccttgca gcacttcctc
    21721 ggcaagctgc ttcccgatct catgggcctg ttccggcgtg gcttcgccgg gggcaaagga
    21781 ttgtatcagg tggtggccca gattattgcc cttttggtat gcctgcgcca gcagcatttc
    21841 aaattcaatg tccgctgttt cataagagca ggcaaaggag gccaccagca gcttgccgtc
    21901 agttttgtcc gggttctcga tgtagtcaag ggcttttttc agcgttgact tgataggatg
    21961 tatctttgta actgccatat ctgcgccagc gcccccttta tatcctcgat gtcctgtgcg
    22021 tacaccggcc ccataccgtt cagcctcttt gcaatctgat tgacgttgac gccgatcttc
    22081 tgtatctcgg cggtctgcgc ccggatgtcg ctggtgtcca tgttgacgat atagccgtca
    22141 atgagcatct tccgggcgta ggctgaaaag ttcttcgtat gcagcagcgc cattttctgt
    22201 tcaatcatag cccgttcctc cggcgtcaca gggacgcgca gcacgatttt tcgctttctg
    22261 tttgccatgc ttcaccgtcc tttccattca ggggtttggg gatttcccca acaagcaaaa
    22321 cggacgcggc ccggcaggga cggggcaggc cgtttttccg ggagtggcta cactccctat
    22381 gcttgctacg ttactgtttc tccactaaca agacgtttcc aaagccccaa actacccgca
    22441 aaatagccct ctggaaagaa aaaagtgcaa aaaaatagaa gctacgctgt gcagctcccg
    22501 ctaaaagtaa aacagaggat gccagttaca catcctccgt ttcattcttc ttcggctttt
    22561 caattagtat ggcgctgtca atcgcgccct cgatgatcgg caggtattct tcggggcaga
    22621 gtttcagctt gtggctgacc ctttgccgca gttcgctttc ctcccgcatg atctccgggt
    22681 taaagtagcg ttccacagga agcccgcagg ctttaataag ctgtatgaca accggcagac
    22741 tgggaatggc cccgtcgttt tcgatgttgg caagatagcg ccagtcaata tttattttct
    22801 ctgccattgc tttgcgcgaa acgcgctttg cttgccgggc cgctttcaca tccgcgccga
    22861 aagtttcaaa gccgggacaa tcttcaatat tcgccatatc gcacctccca cttacattgt
    22921 atatttcata ctctgcccgt ggaatgttgg tatatgcacc tttcttaaag tttatatttc
    22981 acattttgtt tcttttattc ttcgagcaaa agaattataa tgtaccctgt ggaggtgtac
    23041 tatggactac atgacgttga aagaggcagg cgagaaatgg ggtgtgcccc cacgatggat
    23101 aaactactat tgtgctgcgg gacgcatccc cggcgctgta aagatggcga cgatctggct
    23161 cattcccaaa actgcggaaa agccgattga caggcgtaca aagaaaggaa aggcggttac
    23221 tgaatagtat aatgaaaccg gcgcattttg gatatggttt tgaagagaga gaaagcatgg
    23281 tattttgtca cggaaaaact ataagatgat agatggcagg ctgctccaga ccgataaaaa
    23341 gtattctcat ctgaaacaga aagaaaagat ttacacatgg atgtatgaag aagcgaaact
    23401 ttatcatgac agaaatggaa aatgcccgaa acaaagacga ggaagctgct gctgtatcag
    23461 ccgtatatgg tagaattgaa aaggccggca tctggattct ttatggtgaa gtctccagac
    23521 attggataac tgctggtcta atgcgccttt gagtatgggg tgattgattt taataccggg
    23581 gaggtacggc tttttgagga aaatattctg ggatttacat ttggcgatta ttacatttcc
    23641 tgtaactgca aggacacgga tgatgaagat atgaggaata tagacttgct gattttctac
    23701 tgtcttgaac ggtattagga ctgaatctgc tgcgcggagt gcggcaagag cgaccggagg
    23761 gagtacacac aggccctgcc tgtgtgaaaa gccataccgc aaggtgcatt cgcaaaaaag
    23821 aggccgcgca ttttgctgag gcttggggtg tccccaacaa gcaaaacgag aacgccgcag
    23881 gcgggcaagt ggtggatcag aatatccgta aaggatattc tgatccattg cttgccagta
    23941 ttatcgggat gttttgacaa ccgctgtaat cgaaacatcc caaacattaa gaaggtgtta
    24001 attattatta tcttgattta attcgtttaa accaataata tagatacata caccatgaaa
    24061 agaagcgagt atcccaatga cggatgatta aaatccgaaa agaaatcgct ttgaaactac
    24121 agggaaacta cagactgtta tgttatcttc ttaaaatgga gggattttta tgtcgatacg
    24181 aattctactt gtcgaggatg atgatcatat ctgtaataca gtaagggcgt ttctggctga
    24241 ggcaggatat caggtggatg cctgcacaga tggaaatgag gcatacacca agttttacga
    24301 aaacacttat caactggtta ttcttgatat tatgctgccc ggtatgaacg ggcatgaact
    24361 tttgcgtgaa tttcgtgcga aaaatgatac tcccattctg atgatgacag ccctgtcgga
    24421 tgacgaaaac caaatccggg cgtttgatgc agaggcagac gactatgtaa caaagccatt
    24481 caagatgcag attttactaa agcgggtgga agccctgctg cggcgcagcg gtgcgctggc
    24541 aaaggaaatc cgtgtcggca ggctgacact tctgccggag gatttcacgg tactttgtga
    24601 cggtacagag ctgcccctga cacgaaaaga atttgaaatc cttttgctgc tggtgcagaa
    24661 caaaggcaga accttaacgc atgaaatcat tttgtcacgt atatggggat atgactttga
    24721 gggtgatggc agcacagtcc acactcccac actcatatca aaaatttgcg ggccaagctg
    24781 ccggaaaata tcatcaaaac cattcgcggt gtaggatacc gattggagga atcataataa
    24841 tggaaagaaa agggattttc attaaggttt tttcctatac gatcattgtc ctgctactgc
    24901 ttgtcggtgt aacagcaaca ctgtttgcac agcaatttgt gtcttatttc agagtgatgg
    24961 aattacagca aacagtaaaa tcctatcagc cattggtgga actgattcag aatagcgata
    25021 ggcttgatat tcaagaggtg gcagggctgt ttcactacaa taaccaatcc tttgagtttt
    25081 atattgaaga taaagaggga agcgtactct atgccacacc aaatgccaat acatcaaata
    25141 gttttagacc cgactttctt tatgtggtac atagagatga taatatttcg attgttgctc
    25201 aaagcaaggc aggtgtggga ttgctttatc aagggctgac aattcgggga attgttatga
    25261 ttgcgataat ggttgtattc agccttttat gcgcgtatat ctttgcgcgg caaatgacaa
    25321 cgccgatcaa agccttagcg gacagtgcga ataaaatggc aaacctgaaa gatgtaccgc
    25381 cgccgctgga gcgaaaggat gagcttggcg cactggctca tgatatgcat tccatgtatg
    25441 tcaggctgaa agaaaccatc gcaaggctgg aggatgaaat cgcaagggaa catgagttgg
    25501 aggaaacaca gcgatatttc tttgcggcag cttctcatga gctaaaaacg cccatcgcgg
    25561 ctacaagcgt tctgttggag ggaatgcttg aaaatatcgg tgactacaaa gatcattcta
    25621 agtatctgcg cgaatgcatc aaaatgatgg ataggcaggg caaaatcatt tccgaaatac
    25681 tggagcttgt cagcctgaat gatgggagaa tcgtacccat agctgaaccg ttggacatag
    25741 ggcgcacggt tgccgagttg ctgcccgatt ttcaaacctt ggcagaggca aacaaccagc
    25801 ggttcgtcac agatattcca gccgggcaaa ttgtcctgtc cgatccgagg ctgctccaaa
    25861 aggcactatc caatgtcata ttgaatgcag ttcagaacac gccgcaggga ggcgaggtac
    25921 ggatatggag tgagcctggt gctgaaaaat gccgcctttt tgttttgaac atgggcgttc
    25981 acattgatga tactgcgctt ccaaggctgt tcaccccatt ctatcgcatt gatcaggcgc
    26041 gaagcagaaa aagtgggcga agcggtttag gacttgccat cgtacaaaaa acgctggatg
    26101 ccatgagcct ccagtatgcg ctggaaaaca cctcggatgg cgttttgttc tggctggatt
    26161 taccgctcac atcaacattg taaatattta aaacttaaat gattttgacc gacaggtata
    26221 accctgccgg tctttttttg ctgctacagg aaaactacag actggctaca gggaaagtac
    26281 agatgcgctt gccataataa caatcgtacc agccacaaat cgtagtttta ttgcaaagga
    26341 ggcattcgat caaatggaaa aaagcaacta tcattccaat gcggatcatc acaaacggca
    26401 tatgaaacaa tccgtggaaa agcgggcttt tctatgtgcg ttcattattt cgttcacagt
    26461 ctgcacgctg tttttggggt ggagactggc ttccgtattg gaggcaacac agataccgcc
    26521 catccctgca actcatacag gcagcagcac tgacgtagtg gagaatttgg aggaaaacgc
    26581 tcttgccacc gccaaagaac agggagatga acaggaatgg agcctgattt tagtgaacag
    26641 gcagaacccc atccccgcac agtacgatgt ggagcttgag caactatcaa atggtgagcg
    26701 gatagatatt cggatttctc cctatcttca agatttgttt gatgccgcaa gaactgatgg
    26761 agtttacccg attgtcgcat ccggataccg aacaacagaa aaacagcaag aaattatgga
    26821 tgaaaaaatt gccgaatata aggcgaaagg ctacacctct gcacaggcta aagcggaagc
    26881 agaaacttgg gtggccgtgc cgggaacgag cgagcatcag cttggtcttg ctgtggatat
    26941 caatgcggac ggaattcatt caacaggcaa cgaggtttat agatggctgg atgaaaacag
    27001 ctatcgtttt ggttttattc gtcgctaccc accagacaag acagagataa ccggtgtgag
    27061 caacgagccg tggcattacc gatatgttgg catcgaagct gccacagaga tgtacaacca
    27121 aggggtttgc cttgaggaat atttaaaacc agaaaaatga gaaaaggatg ttatgttatg
    27181 gacagaaaaa gattgacaca gcgtttcccg ttcctgcttc caatgagacg ggcgcagaga
    27241 aaaatgtgct tttatgcggg aatgagattt gacggctgtc gctatgcgca gacgataggg
    27301 gaaaaatcgc tttcccattt gctctttgaa acggattgcg cattatacaa ccacaatacc
    27361 ggatttgaca tgatatacca ggaaaacaag gtgttcaact taaagctggc agcaaagacc
    27421 ttaaacggct tattgataag accgggggaa accttttctt tctggtggct ggtacgccat
    27481 gcggacaaag atacccccta taaagacggc cttacggtga ccaatggtaa actcaccacc
    27541 atgtcgggcg gcggtatgtg ccagatgagc aatttactat tttggatgtt cctgcatacg
    27601 ccattgacaa ttatccagcg cagaggtcac gaagtaaagg agtttccaga gccaaacagt
    27661 gacgagatta aaggggtgga tgcaaccatt tcagagggct ggattgattt aaaagtgcga
    27721 aacgataccg actgcaccta ccaaatatgg gtgaccttag atgatgagaa aatcatcggt
    27781 caggtgtccg ccgacaaaga gccccaagca ttatacaaaa ttacaaatgg cagtatccag
    27841 tatgtccgtg aaagtggcgg gatttatgaa tatgcccagg ttaaacggat gcaagttgcc
    27901 ttaggtaccg gggaaataat agattgcaag ctgctttata caaacaaatg caaaatctgt
    27961 tatcccctcc cggaaagtgt ggatattcag gaggagaacc aatgagaaaa agtgtgggca
    28021 ttactgtttt tggatgcgaa caggatgagg caaatgcttt ccgcgcttta tcgccggatt
    28081 ttcatattat ccctacgctg attagcgatg cgatatcggc agacaacgca aaattggccg
    28141 ctggcaatca atgcgttagc gtaggccata agtccgaggt ttccgaggcg acaattcttg
    28201 cgctgagaaa ggtcggggta aaatacattt ctacccgcag catcggctgc gatcacattg
    28261 atacgactgc cgccgagaga atgggaatct cggttggcac ggttgcgtat tcgccggaca
    28321 gcgttgcgga ttatgctttg atgctgatgc tgatggccat acggggtgca aaacccacca
    28381 tgcacgccgt ggcgcaacaa gatttcagat tggatcgtat ccgggggaaa gaactggggg
    28441 atatgactgt gggagttatt ggaaccggcc atatcgggca agcggtcgtc aaaaggctgc
    28501 ggggatttgg atgccatgtg ctggcctatg ataacagccg aaaaatggat gcagattatg
    28561 tccagcttga tgagcttcta aaaaacagcg atattgttac gctccatgtg ccgctttgtg
    28621 cggatacccg ccatctgatc ggtcagaagc aaattggaga gatgaagcaa ggcgcatttt
    28681 taatcaacac cgggcgcgga gcgcttgtcg ataccggggc gctggtggag gcgttggaaa
    28741 gcggaaagct gggtggtgcg gcactggatg tgttggaggg cgaggatcag tttgtttata
    28801 ccgattgctc gcagaaagtg cttgaccatc cctttttgtc gcagctttta aggatgccga
    28861 atgtgatcat cacaccccat acggcgtact acactgagcg tgtgctgcag gatactacag
    28921 aaaaaacaat caggaattgt ctcaattttg aaaggagttt acagcatgaa tagaataaaa
    28981 gtcgcaatca tcttcggcgg ttgctcggag gaacatgatg tgtcggtaaa atccgcaata
    29041 gaaattgctg cgaacattga tacggaaaaa ttcgatccgc actacatcgg aattacaaaa
    29101 aacggtgtat ggaagctatg caagaagcca tgtacggaat gggaagccga cagtctcccc
    29161 gccatactct ccccggatag gaaaacgcat gggctgcttg tcatgaaaga aagcgaatac
    29221 gaaacacggc gtattgatgt ggctttcccg gttttgcatg gcaaatgcgg ggaggatggt
    29281 gcgatacagg ggctgtttgt attgtctggt atcccctatg tgggctgtga tattcaaagc
    29341 tccgcagctt gcatggacaa atcactggcc tacattctta caaaaaatgc gggcatcgcc
    29401 gttcccgaat ttcaaatgat tgataaaggt gacaagccgg aggcgggtgc gcttacctac
    29461 cctgtctttg tgaagccggc acggtcaggt tcgtcctttg gcgtaaccaa agtaaacggt
    29521 acggaagaac ttaacgctgc gatagaagcg gcaggacaat atgatggaaa aatcttaatt
    29581 gagcaagcga tttcgggctg tgaggtcggg tgtgcggtca tggggaacga ggatgatttg
    29641 attgtcggcg aagtggatca aatccggctg agccacggta tcttccgcat ccatcaggaa
    29701 aacgagccgg aaaaaggctc agaaaatgcg atgattacag ttcccgcaga cattccggtc
    29761 gaggaacgaa atcgggtgca ggaaacggca aagaaagtat atcgggtgct tggatgcaga
    29821 gggcttgccc gtgttgatct ttttttgcag gaggatggcg gcatcgttct aaatgaggtc
    29881 aataccctgc ccggttttac atcgtacagc cgctacccac gtatgatggc cgccgcagga
    29941 atcacgcttc ctgcactgat tgacagcctg attacattgg cgttaaagag gtgataagta
    30001 tggaaaatgg ttttttgttt ttagatgaaa tgttgcatgg cgtccgttgg gatgccaagt
    30061 acgccacatg ggataacttc acaggaaaac cggtggatgg gtatgaggtg aatcgcatca
    30121 tcggcacaaa ggccgtggcg tttgctctgc gcgaggcaca aatccatgcg gctgcgcttg
    30181 gctatggctt gcttttatgg gatggatatc ggccaagaac tgcggtggac tgcttcctgc
    30241 gttgggcagc gcaaccggag gacaatctca caaaagaaaa attttacccc aatatagagc
    30301 gagccgagtt gattacaaag ggttatgtgg cctcacaatc cagccatagc cgtggaagcg
    30361 caattgatct tacgctctac cacctggata caggggaact tgtttcaatg ggaagtaact
    30421 tcgattttat ggacgaacgg tcgcatcata cagcaaaagg gataggggat gcagaggcac
    30481 aaaatcgaag atgcttgcgt aaaatcatgg aaagcagcgg atttcagtct tatcgctttg
    30541 aatggtggca ctataagttg attgatgagc cataccccga tacctatttt aattttgccg
    30601 tttcataatg aaagtattgg ttttgtatca gattttggat ttgataaaat ctatgctggt
    30661 catggacaat cttttactgg aaagtggtta aagcatcttt tgtaatacat tgcaaattat
    30721 agtattcaat ctgcccagcg gcagaaaaga aaaaaaccgc tgttgggcag acctttccca
    30781 cgcccatttt gtattttgct ggcggcggtt ttgagaaatg aaccgccccc tgtcaagtag
    30841 acagatgaaa aaagaaaaat tacgccacga gggtctggtt cctgtaagca aggggagcca
    30901 ggccctttaa cttagcttga agccgccttg tattgtaaaa gtctatgtat tcgtcgatgg
    30961 ctttcttcaa ttcctcgaag gtatgaaatt gccggaggta gtacatttca gatttgagaa
    31021 tcccccagaa gccctccata gggccgttgt cgatgcagcg gccgggccgg gacatactct
    31081 gcgttgcatg gataccgtcc agctttgctt tgaactgccg gttggtgtac tgatagcccc
    31141 tgtcgctgtg aaacagagga tgggcgccgg ggttggctct caccgctctg tcaaaggtgt
    31201 caaaaacaag ctgattgttg ttggattggc ccagtgcata cgcaacgatg cttttgtcat
    31261 ggagatcaag gatcgcactc aggtatgctt tcttcccatt gataagtttg aattctgtga
    31321 catctgtcag ccatttctca ttgggaccat ttgccgtaaa ttctctgttc aatacgtttt
    31381 ccgcggtaac acgcggttct aatatgacat agtgcttttt ctttcggcgt atcacggcct
    31441 gcatatgtac acttttcatc aggcggtaaa tccgcttgtg gttgtagttt cttttgagcc
    31501 ggctgttcac attcagcgtc atacggcgat aaccgtagat gccttttact tcgctgtaca
    31561 aacggatgat ttcttccagc aagagggtat tttctttttc tgaggcactt tcttcccggt
    31621 gaagccattt gtagtaagaa cagcggggaa tcccggataa cgcgcataac gatgcgattt
    31681 catatccttt ttcctgacga agcgtatgga tcgcctcata gaccttcctg cggcgtgtga
    31741 gcctgtttac cgcctcccct ccagttcgtt caattttttt aagaaatcgt tctccatttg
    31801 aagccttctg ttttccgctt ccagctgccg cagctttgcc gcggcttttt cctcctcgct
    31861 gtattcggaa ggagggcggc gtctcccccg gcggtcaagc aacccagctt ctccctgctc
    31921 ctgatatttt ttgacccagc tatagatctg ctgataggtg atcttgtact gttcgctggt
    31981 ccgccggtag tccagttgat gttctaagca gtatagtact gcctgcagct tttgctcctg
    32041 cgataatttt tctttctcgg ccatatctgt ttcctcccgc gctctgcgtg tcttgaactc
    32101 tctatggcct tgattataca acgtaatcca ttgttgtaaa accgcatggg aggaaatccc
    32161 gtatttgcgg cacatggcat ccagcgagcc tcctccgccc atgtagtctt ctactgccgc
    32221 cagcttcagt gaggatggat aacttgtaac atgctttttc ggatgcagac ccgcaggccc
    32281 ctccgtctga tatttccgca gccatgcctg gaaagtgctc aatcgtatgc ctaactcttc
    32341 ccggatctgt gtggaaccct tacggatcgc taggtagtcc tccacagcct gaatctttac
    32401 ctccggcggt attttgcctt ttaaactcat gataattgct ccctccgttt cgacagttta
    32461 atttttttgc tgtctacttc agagggagca tatcaaaatc aaggccgccg ttcttttctg
    32521 gcagcaaaag gtgtggcggc ccataggagg atgccgctat gctgttttgc tattgccatg
    32581 ctctaatcca cttccaccac tcaaaaaaag ccttgcccct ccacagggct attccggtta
    32641 tgagtatgta ctataccatg taaatcagca gcatagcaaa gcattttctc gcccggtagg
    32701 tttgcgacct gccgggcgat ttttgtcgtc ttttgcggcc cggctgtccc caaaaaaatt
    32761 ttttgagaaa ttttcaaaaa ggaggtattt agcgggtagc tggcggcctt gtcaaagtct
    32821 tgttagcgga aagggagaga accacctaag tcccccacag aaagggggtg agaagatgga
    32881 tgctatcccc cgcgactatg aagcgcggtg catgttcgat gctttttgca agacggtact
    32941 gcgtaacgag gccaaaagct acttggctga aatgaagcgc cgccgtgacc gtgaagtgtc
    33001 actaagctct ttgtcacagg cagatttgga caagctctgt acagtagatc actaccccag
    33061 cgacagcttt acgttttcgt cgcatggcta tgacctacat atcaacaatg agcttgttgc
    33121 cgaagccttt gccgcgctgc ccagcatgga gcagagtatt ttaatcctgc actgtgttct
    33181 ggatatggca gatggtgaga tcggcggcct tgtgggaatg tcccggagtg ccgttcagcg
    33241 gcacaggaca aataccttat cggagctgcg gaaacagcta aaggcgttga tgccgaaggg
    33301 aggttagacg cggatgaaga acccgaacta caagggcatt gagcttttgc ccctttcggt
    33361 gatcgaggcc gcccgcgctg gggatgccgg agccgtagag cgcgttttgc agtattacag
    33421 cggttacatg aacaagctgt gtacccggac gctctatgac gaaaacggct atccgcatgt
    33481 gtgtctggac gaggccatga agcgccgtct ggaaatcaag ctcatccacg ctatcgtcac
    33541 gaactgacga accggccccg gcagcatgaa acggcttacc ctttccccgt ttcggtgttt
    33601 tcccggtggc ctgatcgcac cttgataaat atgcccgcaa gataacggcg gcgcatcata
    33661 gggcatacgg acacattctg tctgtgccga gccggacggc tggtgcgcca tgatactttt
    33721 ccctacggga aaagcggagc gaggaacacc acgccgcaat gtgagcgtgg cggcttgcgg
    33781 gcgacgacgt acaggcagga ctatgatact cccttatagc cacagtccga gcgttaaaag
    33841 cgtcgcaggc aatgggtagg gccgcgtgag aaccacgcgg gggtgaaagt cccgtggagc
    33901 tgtgccagca gccgtccgtc tatgccccta aatcacacaa acctttgaat ttgtgaaagg
    33961 gggctgcatt atgaaccatg atgtgcctat ttgggaaaaa tacacgctga cgattgagga
    34021 agcgtctaaa tatttccgta tcggagaaaa caaattgcgt aagctggccg aggaaaatcc
    34081 ggcctctggc tgggtcatta tgaatggcaa ccgcatccag atcaaacgca agcagtttga
    34141 gaaaataatt gacactttgg acacaatcta atagtgaaaa agggccgcag atatgttata
    34201 ataatttata agcatatatg cggctctttc cgtcatggaa aggagcatga cgagatgtca
    34261 gaaaaaagac gagacagcaa aaatcgcgtt ttgcggtctg gagagagcca gagaaaagac
    34321 ggacgatatg cttacaaata tgttgatacc tttggaaaac cgcagtttgt ttactcatgg
    34381 aagttagtgc ctacggataa gaccccggca ggtaaacggg aggatatttc actccgggaa
    34441 aaggagaaag aaatccagaa agaccttgac gacgggatag acaccatcgg caagaaaatg
    34501 accgtctgcc agctctacgc caagcagatc aggcaccggg gcaatgtgaa gcacaacacc
    34561 aaactgggcc gggaacggct gatgcgtatt ctggaacaag atcggctggg gagctgcccg
    34621 attgacagtg tgaagctgtc cgacgcaaag gaatgggcgc ttcggatgaa agaaaagggc
    34681 ctttcttata agaccatcaa taacgacaag cgttccctga aagcggcttt ttacaccgcc
    34741 atacaggacg attgcatccg aaaaaacccg tttgacttcc agcttagtga cgttctggac
    34801 gacgatacgg aaccaaaggt gcctcttaca ccagcacagg aagaaagttt tctgtccttt
    34861 attcaaggtg ataaggttta ccaaaagcat tatgacgcaa tcgttattct gctggggaca
    34921 gggcttcgca tttcggaact gtgcggactg acggacaaag atttggattt tgaaaaccgg
    34981 gtaatcatcg tgagccacca gcttttgaga aataccggcg tcggttacta tattgacgaa
    35041 cccaagaccc aaagcggcgt cagaaaaatc cccatgaatg aggaagtgta tcaggcgttc
    35101 cagcgagtga taaaaaaccg caaaggtgca aagccgttca ttattgacgg gtatgccaat
    35161 ttcctgtttc tcaaacagaa cggctatccc atgacggcgg tagattatgg cgggatgttt
    35221 ggcaggcttg tgaagaagta caacaagagc catgaagaag cgttgccgaa aacgacgacg
    35281 ccccatgcta tgcgtcatac attctgcacc cggttagcca atgcgggaat gaaccccaaa
    35341 gccttgcagt acattatggg acattccaat atcaccatga cgctgaacta ttatgcacac
    35401 gccacattcg attccgcaag ggcagaaatg gaacggctgg cagcctgaca caaggccggt
    35461 tttactactc catttactac ttttgagggg gaaatggtga gaggtaataa gaacatttgt
    35521 aagtatctaa caatatcaaa aatgccggaa aagcccggaa acacgggcta taccgacatt
    35581 caagaacttc taaaaagata atctaaaatc ccatataatt ttcctcctaa aaaaatagca
    35641 caatggcaaa gatcgtaaga gagatcataa gccccaatga atgactaaaa agaaaagcag
    35701 aatcacatgc catatcatcc catatttctt caagaaaata aataagcggg aaaaagggaa
    35761 ggaatagtag aacatcgatc cagcaccatt tttttcttct tttgaaataa cataaaacaa
    35821 taaacatgaa cgtgcaggga agtaaaaaca aagctttact cataactaac aaaataccag
    35881 ctccttttat ttttttctcc cccacattca aagagtaaca atggtatgta cttaataata
    35941 gaaaaatgga tcaacttgta tgaataacag ctgaaaagat ctgcaccaaa taaaaaactt
    36001 ccagccaaaa ctgactggaa gtttattttt ttctgaaata ttctaacaca aatatgaaaa
    36061 agatggccga agctaataag ccgatttcgt agcaaaacaa tgagatacca gagtcgtaat
    36121 aataaaatcc tctaatctta tggataaaat agatcatcgg acaagcagat aagagtaagt
    36181 atttgttagg ccttttgaat aagtaaaaga aagcactggt cattatgata aatggtatcc
    36241 aaaacaaaag tttacttaag acaatcaaat gatcaactcc catatttttt accaagagag
    36301 atcataacat ctttatcgta aaaacaaaat atttttttgc tcattttgaa aatttaaaca
    36361 tactcgttct tatccaaata tataaaaatc gcccctcatt catcgagagg cgataagttc
    36421 taatttaact caattttttt tgtttctttt ggctgataag ctgttttagg taataccaat
    36481 ttcagcacac cgtttttaaa ggttccagtg attttttctt catcaacatt aggaagatag
    36541 aatgaacgtt tgtaagagac cgaatgtctt tctttccgta catagttgcc atcttcttta
    36601 tcttctgtat gggactcatg attggcggag atcgtcaaag tattatctga atattcaact
    36661 gtcgtgtctt ctttgtcaca gccaggcata tcagcagtta gtttgtattc tttttcattt
    36721 tcgactaaat ctacttttgg ataacttgaa acacctaaaa aatcattgaa tgctggacta
    36781 aacacgtcat tgaaatcagg gaacatgtcg cgaatgctag gtacatttgc catttttgat
    36841 catcctttca ataagtattt tttatttatc cctatttacg taataactct attttgaaaa
    36901 aaatgcaagt ataatggtta gctttgtgaa tatttcatct aatacgatac aaaggctatc
    36961 tttctaattt gaaaaaggga tcattaaaat aataagaaga aaatatgtca aaaggtaaaa
    37021 aagtaaattc ttctatccaa atatagaaaa attgaatcta gaaaaccgcc cgacaataaa
    37081 tttcattaac tcagttgacc gaaaatgaag tagataccaa aaatttatca agagagcaag
    37141 tagagaagtg agtatattca taagagaaat agctgtaacg aattattagg ttaatgcaaa
    37201 tgcttattta tactccgtat aactttctca gaaaaaatga taccaataat gacttgctag
    37261 tactgtaaat ccgtataata tcatctaatc aaaatggtgt tccatttgca tctgtcatcg
    37321 taatcgatcc ttcgggtgac actcgataaa aaccaacgct tccagatcct ccttgggaac
    37381 ggatttgttt gctggaagct gtgaacaaat aatctcgatc aattttttga taaaagctaa
    37441 taacaatatc gtcactttgt aattcggaac ggtgggcaat aagaaataga agtgctttgt
    37501 cagagttttc taatggaaga gaaggtacag tgctttcttg catttcagat gttgaagaaa
    37561 tgctaaacga ctgaatactt gattggctat gttgcgaatc tacattacca gtagaagtat
    37621 tagtaatgga agtagtagaa gtacttttag ttgaaccaac ctcagttgtt gaaatggctg
    37681 ctgaagattc tttaacagaa ttctttgttt tatatccaca ggcagaaaaa agtattacag
    37741 ttcccaacaa gcctgcgtaa atccctttat gcatgatagc tcctcctaaa atagattatc
    37801 tttattcagt atatcactta aacaaagaaa agcagatggt ttaaaatgaa aaaaccttcc
    37861 caataaaggg aaggttcatg aagataataa cttttttaat aattatagct ttctaatatc
    37921 tttcatcaac ttttctagta atcattggga aaaatttcaa gcagtagatc gcacaaatac
    37981 ctacgacgat ataaatgatc ttcgcaaaaa tagttgttga accaccagca atcatagcta
    38041 ctaaatcaag ttcgaacaag cctaccaaca gccagttcaa ccctcccaca atcaataaag
    38101 ttaatgcaat agcatctaaa gttttcatta gaattctcct ttcttaaatg ataaaacaat
    38161 tatatatcaa aaagagaaac attgcttata taacgctcag gaattttagc aatctatgca
    38221 aaaagatgaa aatttttttt gctcttccat tagtacacat tttttagctg aagctgtaaa
    38281 tttcatgtct taattttttt tagtgagctt ttatactgga tttgtaagaa aaaagaagga
    38341 gaggatcaca tatgtcggat tggttggata ttgcaggaaa agtagtaatt gtaactggag
    38401 gttcctcagg aattggaaga agtatcgtcg aaaatttgtt gaagcagaat gcacaagtag
    38461 cgaattttga tgtaactgaa tgccgtatac agcatgaaaa cctactatct ttaaaagtcg
    38521 atgtgtcttc taaaacggac atagaagaag ggatatataa agtaatgaaa catttcaaga
    38581 cgatcgatgg tctagtcaat aatgcaggaa taaatattcc ttctcttcta atagatagaa
    38641 atcatccaaa aagcaagtat gaattatcag aacaagtttt tgataagatg atcgctgtca
    38701 atcaaaaaag tgtatatttg atgagccaag cagttggaag aatcttggtg caaaagggat
    38761 caggagtgat cgtgaatctt tcttctgaat caggattgga aggatcagaa ggacagagct
    38821 gttatgctgc tacaaaagca gcgatgaaca gttttactcg ttcttgggca aaagagttag
    38881 gaaagaggaa tgttcgtgtg gtaggtgttg caccagggat actggaagaa accggtttga
    38941 gaacacaaga atatgaagaa gctctttctt atacgagagg tataagcgtc gaacagctga
    39001 gaaacggtta cagtcgaaca agtacgatcc ctctaggaag gagtggtaag ctgcaagaag
    39061 tggcggatct agtctgctat tatctttcag aaagatcaag ttatatcact ggagtcacga
    39121 ctaatatttc aggaggtaaa acaagaggat aaaggatagg ggggacgcac gtgacgctag
    39181 taaacagatg gtaccaatta ttgaaattac tagtcactca caagaaagtt tcaatccagg
    39241 aagtgcaaaa agaaatcaaa accagtaatc aaaccataaa aaaagatatt ggggagttga
    39301 atgaagaaat tgccggtatt gcaaaaatca tccaaaaaaa caaacatttt gaacttgaaa
    39361 tttatgattt tgattttttt gatgagatca tgcaaggaaa attgaaaaca gccagtgatt
    39421 tcaattcggc atccaaaaga atcgcttatt tactaaatag gctgatcgaa acagaggatt
    39481 tcttacgtat ggatgatttg tctgaagaag tcggtgtcag cagaggaaca gtggtcaagg
    39541 atctaaagac attaaaagag atgatcaatg attttgatgt aaagattaca ggcacaccaa
    39601 ataaaggact aagaataata ggagatgagc tagaattacg actactttta ttttattttg
    39661 tttctcccta cttttcagag acgaaaacag aacagtttct agaagaaaaa caagcaatca
    39721 aacattttca gaagaagacg aatgctagta aacaagaaat caccctttta acgaaagtta
    39781 tcggtatttc tttgaataga atctctagta actatctgtt gaagaagccc atcaatcgtt
    39841 acagtgggtg ttttgaatat atccaagaat tcaatgaact gatcaatcag ttggaagaaa
    39901 tctatgaact aactttgagc caatatgaaa aagattttat cagttttcct ttgaacatca
    39961 tcaacacaag tataacgatg ataaggaaag ctaactctga tttgcgctta tatttcgacc
    40021 ccatgatgat gcgtattcga cgattagatc tcattgatct ggatgagtcc ttcttatatc
    40081 aagagatgaa acagcacctc tctttattga taaatcgggt aatttttcgt tatcgattga
    40141 ctgatttatt ttatggtgag atagaaaaaa agtatccttt ttcttaccaa atcgctagcg
    40201 aatgcataca agcattagaa gctatcttgc aacataagat ctctaaagta gagatcagct
    40261 acctaacgtt gtatttggag ttagcatttc gatatcaaaa agagcatttt tcctctaaaa
    40321 aagtagccat tgttcatacg acaggaaaag gaacagcatt aatgatccaa agacaaatca
    40381 aaaggatatt ggggagggat atacaaataa cacatgtttc agaagaacaa tatgaaaaag
    40441 tagatctaaa caaatatttc gctgttttta caacgattcc gttaaaaaat gtaaatgaag
    40501 agacaccggt gatccatttg actaatcttt ttgatgactc ttggttaagg gcacaatggc
    40561 aaaaagcaaa aaaaaagcga ttagccgggt ttcattctac tgtttttttc tatcaaatat
    40621 ggaaagaaaa aaagacgtat caagaatatt taaacaaatt agtcgctcct ttgatcagag
    40681 gctattatgt ggatgaagaa tttgaaaaaa ggatcttgaa aagagaatat cagcaaagca
    40741 catttgttaa caaagaattt gcttttcctc acacaaccaa ccagctatct caacacattg
    40801 ttttatcagt agggctaata actgatcaag gtttagcaga aacaggagga aatggagtta
    40861 tttttttatt tgccatacct gaagaaatga cggctgaagt tgagaatgaa ttatttgagc
    40921 tatacgatat cctatttgaa ctcataaaaa tgggaataga tgagtttatt gaattagcac
    40981 cgacacaaga atcattttta aaactaatca aaagtaaagg agagagactt catgaatgat
    41041 atgtatctat tcatagtttt cgcagcttca gcttatgtgc tgcaagcagt tatcagttgg
    41101 aagcaactaa agcatttcaa tcaaacatat agcgaactgc gaagaaaagg aaaagtagcg
    41161 attggtaaac gacctgggaa aatccgttca ggcacgattg ttatgctagc tatagacgat
    41221 catgatcaag ttttagaagt cagaaaaatg caaggggtta catctttggc aaaatttcat
    41281 tcattacctg tctatgcggg tgaagatatc cattacatcg atcgttacca tccctcagtt
    41341 cgagcggaag atcgtttgac acaagcagct ttagaaaatg ccagagaagt atacttacga
    41401 atagaggcag gaaatgaaaa aagtgaacaa ccttcattgc cttttattgc tatgctcaat
    41461 aattttcgga tatggaaaag tacgatcaaa gaaaaaagga gtgacaaacg atggatatca
    41521 taacaaaatt tgctgaggga tttatgaggc tgttccaaac aggtgcagaa acattcataa
    41581 gctggatgac aggaatcgta ccagttgtct tgatgctgat ggttgcaatg aatacgctga
    41641 ttgctttttt aggtgaagac cgagtaacga aagtagccaa attatcagcg aaaaatccgc
    41701 ttactcgtta ccttgtgtta ccgttcttat ctgcttttat gttgggaaat cctatgtcat
    41761 ttacaatggc tcgtttcctt ccagaatact acaaaccaag ctattatgct gctcaagctc
    41821 aattttgtca tacgagcaat ggtgtattcc cccatattaa tcctggagaa ttgtttgtat
    41881 ggatggggat tgctcaaggg atcgaaacct tagggctaaa ttcgatggag ctggctatcc
    41941 gatacttgct tgtgggactg ctgatgaact ttattggtgg ttggataact gattttacta
    42001 ctggatttgt atgcagacag caaggaatcg ttcttagcaa aaaagtagag ctttcagttg
    42061 attagagaaa ggagagtttt aaatgtatgg tgatattaag atcgtaagag gaaacggcgg
    42121 gtatggaggt cctttgatta ttaggccgac aaaagataaa cacaagttca tctatgtcac
    42181 aggtggaggt gagaggccaa tgatcgtaga taaaatagaa gaactgacag gtatggagtc
    42241 tatcaatggg ttcaaaacat caatacctga tagtgaaatc gcgcttgcaa tcgttgactg
    42301 tggagggact cttagatgtg gaatttatcc taaaaaaggg ataccaacca tcaatatcgt
    42361 ttccacagga aaaagtggcc cattagcaca atatatcact gaagatattt atgtttcagc
    42421 agtagggtta gaacaaatac agccactaat ggaaactgat gataacaaag aaacagtaaa
    42481 tactgcatct ccagataaaa agtacaaata tgatacttct aaaaagataa ccgagcaaaa
    42541 agcagagcag caaaactgga tagcaaaaat tggaatgggc gcgggaaaag tagttgctac
    42601 attcaatcaa tcagcaagag aagcagtaga tacgatgatc cggacagtga tcccttttat
    42661 ggctttcgtt tcccttttga ttggtgtgat acaaggttct gggatcggta atgtatttgc
    42721 taacattatg gcacctttag caggaaatgc agttggatta acaatcattg gctttatttg
    42781 ttcactgcca tttttatctc ctttgcttgg tccaggggca gttattagcc aagtgattgg
    42841 aacattgatc ggtgtagaga ttgggaaagg aaatattcct ccccaattag cattaccggc
    42901 actatttgct atcaatactc aaaatgcatg tgattttatt ccagtcggtc taggtttgga
    42961 agaagcaaaa gctgagacag tagaagtagg tgtaccatca gttctttatt caagatttct
    43021 taacggcatc ccacgagtta tcattgcttg ggtagcaagt tttggattat atagctg
//
LOCUS       DAZUKC010000019        37952 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000019 DAZUKC010000000
VERSION     DAZUKC010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.019"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            167..3445
                     /locus_tag="ACG80T_000920"
     CDS             167..3445
                     /locus_tag="ACG80T_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFS4840589.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3986..5893)
                     /locus_tag="ACG80T_000921"
     CDS             complement(3986..5893)
                     /locus_tag="ACG80T_000921"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFS4840590.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            6023..6898
                     /locus_tag="ACG80T_000922"
     CDS             6023..6898
                     /locus_tag="ACG80T_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFS4840591.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6978..7952
                     /locus_tag="ACG80T_000923"
     CDS             6978..7952
                     /locus_tag="ACG80T_000923"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFS4840592.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8132..9277
                     /locus_tag="ACG80T_000924"
     CDS             8132..9277
                     /locus_tag="ACG80T_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFS4840593.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9414..9758
                     /locus_tag="ACG80T_000925"
     CDS             9414..9758
                     /locus_tag="ACG80T_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFS4840594.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            10029..11315
                     /gene="efmA"
                     /locus_tag="ACG80T_000926"
     CDS             10029..11315
                     /gene="efmA"
                     /locus_tag="ACG80T_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFS4840595.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11453..12052)
                     /locus_tag="ACG80T_000927"
     CDS             complement(11453..12052)
                     /locus_tag="ACG80T_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS4840596.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12456..13577
                     /gene="mnmA"
                     /locus_tag="ACG80T_000928"
     CDS             12456..13577
                     /gene="mnmA"
                     /locus_tag="ACG80T_000928"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFS4840597.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13823..15244
                     /gene="arcD"
                     /locus_tag="ACG80T_000929"
     CDS             13823..15244
                     /gene="arcD"
                     /locus_tag="ACG80T_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFS4840598.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15258..16376
                     /locus_tag="ACG80T_000930"
     CDS             15258..16376
                     /locus_tag="ACG80T_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFS4840599.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16436..17728)
                     /locus_tag="ACG80T_000931"
     CDS             complement(16436..17728)
                     /locus_tag="ACG80T_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFS4840600.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17878..18438
                     /locus_tag="ACG80T_000932"
     CDS             17878..18438
                     /locus_tag="ACG80T_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4840601.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18567..18944
                     /locus_tag="ACG80T_000933"
     CDS             18567..18944
                     /locus_tag="ACG80T_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFS4840602.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18877..20949
                     /locus_tag="ACG80T_000934"
     CDS             18877..20949
                     /locus_tag="ACG80T_000934"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFS4840603.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21160..22107)
                     /locus_tag="ACG80T_000935"
     CDS             complement(21160..22107)
                     /locus_tag="ACG80T_000935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4840604.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22353..22817
                     /locus_tag="ACG80T_000936"
     CDS             22353..22817
                     /locus_tag="ACG80T_000936"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFS4840605.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(22879..23922)
                     /locus_tag="ACG80T_000937"
     CDS             complement(22879..23922)
                     /locus_tag="ACG80T_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFS4840606.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(24299..25264)
                     /locus_tag="ACG80T_000938"
     CDS             complement(24299..25264)
                     /locus_tag="ACG80T_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFS4840607.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25498..26343
                     /locus_tag="ACG80T_000939"
     CDS             25498..26343
                     /locus_tag="ACG80T_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4840608.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            26534..27721
                     /locus_tag="ACG80T_000940"
     CDS             26534..27721
                     /locus_tag="ACG80T_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFS4840609.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            27727..28083
                     /locus_tag="ACG80T_000941"
     CDS             27727..28083
                     /locus_tag="ACG80T_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFS4840610.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            28085..28423
                     /locus_tag="ACG80T_000942"
     CDS             28085..28423
                     /locus_tag="ACG80T_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFS4840611.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    28499..28755
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            28818..29852
                     /locus_tag="ACG80T_000943"
     CDS             28818..29852
                     /locus_tag="ACG80T_000943"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFS4840612.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            29845..30531
                     /locus_tag="ACG80T_000944"
     CDS             29845..30531
                     /locus_tag="ACG80T_000944"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFS4840613.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            30555..31403
                     /locus_tag="ACG80T_000945"
     CDS             30555..31403
                     /locus_tag="ACG80T_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS4840614.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            31600..32373
                     /gene="sufC"
                     /locus_tag="ACG80T_000946"
     CDS             31600..32373
                     /gene="sufC"
                     /locus_tag="ACG80T_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFS4840615.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            32386..33672
                     /gene="sufD"
                     /locus_tag="ACG80T_000947"
     CDS             32386..33672
                     /gene="sufD"
                     /locus_tag="ACG80T_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFS4840616.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            33669..34904
                     /locus_tag="ACG80T_000948"
     CDS             33669..34904
                     /locus_tag="ACG80T_000948"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFS4840617.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            34891..35361
                     /gene="sufU"
                     /locus_tag="ACG80T_000949"
     CDS             34891..35361
                     /gene="sufU"
                     /locus_tag="ACG80T_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFS4840618.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            35366..36757
                     /gene="sufB"
                     /locus_tag="ACG80T_000950"
     CDS             35366..36757
                     /gene="sufB"
                     /locus_tag="ACG80T_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFS4840619.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(36855..>37952)
                     /locus_tag="ACG80T_000951"
     CDS             complement(36855..>37952)
                     /locus_tag="ACG80T_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4840620.1"
                     /translation="WGFETYLGKDPKTGKDIRTKRRRFKSKAEAQTALNRLLVDFKEK
                     QEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILPAFGELRLDKISVSYC
                     QKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTLLPRKIEEEQPLKFYT
                     KDQLQLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWEDIDYFNKTITIGKTIA
                     QDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMILYGYNTNSPKQFLFT
                     TNTNKLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLFESGASIKEVQERLGH
                     KDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
BASE COUNT        12336 a         6988 c         8063 g        10565 t
ORIGIN      
        1 acttattctt tttccgttaa taaatctctt tattatcaac aacttaagtt atattttttc
       61 tttttataaa atacacttat tataattttg ttttttattg acagtaaaaa taaattaggt
      121 aatactttaa gtaaacgctt tctaatttgt tacgggagga atacacttgt taaaaaaacg
      181 tataaaatac agtgcgctaa tgatgaatct ccttatgctc agtaccccgg ttttaatttc
      241 tgtcgttgaa gttgctgcta acgaacaaca gtctgtgaac gaagaaaatg cacacgtgaa
      301 cattgaaggg atgtcaacaa tggaggaaat accaagaact agtgaagata tggatacgga
      361 agaaggaaat gatcctatga tgaaagaatc agaaaatgaa aagacaaaaa agcaatctga
      421 agaagcaaat gaagagtgga aaggggttgt tttcggagaa agcacaagtg ctagtaggta
      481 tgccattgaa ccattagaag acggcgtgcg gctctcttcc acgaataacg gcggaaaatt
      541 ccagtcaagc ggatcagatg gaatgaccta ttattatcaa gcaatcccca aagacatcaa
      601 ctttaggatg cgggcgacga tcgaaataga atcttggacc tatacgaatg cccaagaagg
      661 atttgcttta atggtaagag acgcagtacc aaaaaatcat ttgtttgggc aggcatttta
      721 ttcaaattca tttgccatac taggcagtcg gatcgaatat gtctgggact cggatagaca
      781 agaagtggtc aacacgagcg gcagcaaata tacgatgcga cttggattgg ggacacgggc
      841 aatcacagga atatcatctg aagatccgca agctgcgcca gctcctggga gtgtctcagt
      901 ggaaacaaca cctttagaga cttcagcagg gttcttggaa aaagaaacag gttcttataa
      961 tatattcgga aacggtcatg ggaatctgtt tccggccacg aactcgatta ctaaactgga
     1021 cgtagagatg aaaaaaacga atactggttt agaggcatat tattacgatc ctgaaacagg
     1081 agaagaaatg gcttctgaca tcatgtatga ctgggaaaaa ttatacgctt cagatgaatc
     1141 ggttatttat gcaggatttg ctgcagcaag gaatatgacg atcaaggtag aaggaatcga
     1201 agttgcttac tcagatcctg cgacagatcc tcctgggcaa gaaagaccta ctcgcttgat
     1261 tgatccgatt tacactgtga cctctagtaa tcattcagga aaccaagatc atactatgtc
     1321 ctttagagca aatgctgacg gattattgac aattaaaaat aaagcaacag gagaggtgct
     1381 taagaacgca aaaaacttgg ctatcactgc gaatcgggaa tttgattatc agacaaaact
     1441 gatggaagga acgaacgaat ttactttcag ttttacgcca gataaaaact atccgccaga
     1501 agaatacgta gagatgacta gttatgaaac aaaagagatt cttcatacag tggactaccg
     1561 taaacctaaa tattcgacag tttatgtaac accagaaggg tcagatgcag gaaacggaag
     1621 cagacaaaat ccattatctc tgacacaagc attgagatac gcatatgctg ggcagacaat
     1681 cgtcatggcg cctggaactt attccttcga aagaggattg acgattccaa aaggcgttaa
     1741 cgggacaaga gaaaacccga ttcagttgat tgcagagaaa aatccagaaa acaagcgtcc
     1801 tgtacttgat tttaaaggaa ctggaaatgg gatgacactg ttcagtcact actggggttt
     1861 acgagggttt gacattacaa atagtgcagc tatgcaaaaa ggattacaga tttctggaaa
     1921 tcataatttg atcgaagaga ttcatgctta tcggaatgga aatacaggaa tccaaatcag
     1981 cggtagctca aatgatcctt tcgaagcatg gcccgcgcat aacttagtca agaactgtac
     2041 atcttttgaa aatgccgatc caggatttga agatgccgat ggatttgcgg ccaaactgac
     2101 aataggagaa ggaaatgtat ttgacggttg tatcgcgtat cataatgcag atgacggttg
     2161 ggaccttttt gctaaaaatg aaacaggttc gatcggaaaa gtaatcatca aaaactcagt
     2221 tgcttatcga aacggctggg taccaggaat tgagggagaa ggtaacggta atggattcaa
     2281 aatgggcggt tcttctttaa cgggccctca tgaattgatc aatagtttgt ctttcgaaaa
     2341 tcttgcgaaa ggaatcgatt ccaatagcgg aacagatatt atcgtcaata gcagtacaag
     2401 ttttaataac ggttcatata atgtggctct ttatacctct tcagcaggaa acacagacta
     2461 ccatgcttct ggtatcttat ctttccgaac agagaatctt gagatgagag agcagatccg
     2521 cccattgaat caaaacgaag atcaaatcta ccactcgttg aactactatt ggaatgaacg
     2581 agagaaacaa tcagaaaata ctcttggaca taccatatca aaagattgga tcgagtctct
     2641 aaatacatgt tcgaaagaag gtcagcagcc tgttacgcgc tatgaaaatg gaagtatcca
     2701 tgttcatgga ctgatgcaga ttaaaccggg gaatcgtcaa acagaaaagg aaagagtgga
     2761 tggtcttcca ttatctgtag gtgctatttt tgacggagaa acctcaccgt cttcggaata
     2821 tccagagtat gcaatcgtac aagaaccaaa tcagacaaaa ccaaatgaac aaaataatcc
     2881 gattattata caagaagcaa atggtgtggc ggagaatgca gaaggaaata tcattgaact
     2941 gaagcctttt acagtttttg tgtcaacttt cacaccaaat caaggaacgc ctgtctttga
     3001 agatatgcgt tatttcccag aaaagatcca aatcgttgaa acacagtcaa gtgctcaatt
     3061 ttctaatcat accgctattt tttcagcacc ttcaaaagga gaatatacgc tagaagtcac
     3121 ttatcgttcg gaaatatttg atggggaaaa atggtctgct catacagaat ctgttaccca
     3181 gcaaaaagcg atcactgtgc gagaaactgc cgatgtgccc ccgggaaatg acaaaccaga
     3241 aagagaaaat ccaggaaatg aatcaccaaa tggaaaacca aatggaaaac cttctcatcc
     3301 ggataggaca gcaaaagaaa gtgaaaaaaa tggcactcaa ggactcccaa aaacgggaga
     3361 atttaataac ccgttgctag cattagctgg aggaattttg ttaatcggtg tagttgttta
     3421 tgtcatgaaa cagcgaaaaa aataagtgga atttctcctt ttatgatgta aaacaattga
     3481 ctgaggaaag taaaaataga aggaaatgag accaatagat gtctcacttc cttctatctt
     3541 tttatagatg aggttctcct tgctgctttt gattggctgt tcttattact tgatgcagga
     3601 cagctttttc acaatctctc cactaaacgg tgttcaatca gctgaccctc ggtattagct
     3661 caacaaacgg caaaatatga ggaactattt ttccgttttt ttctctaata ctcaggtcag
     3721 gacgctgatt tcacaacctc tcgataaacg gtgttcaaaa gccagtttct cggtagtaag
     3781 tcaaatttat gcaaaaatgt ggaaagccat tttcacaaaa tttttcttac tacttgaaac
     3841 taagcagctt tttcacaacc tctaatacat ggtattccag aagtaacttc ttcgaaaaat
     3901 aagccgaaat gtcacaaaaa tttgagaagc gattttccca aattccttct tatttcttga
     3961 agctgaccac ttctggcaca acctcttacc agattcgcac tcttttttct ggcgctagat
     4021 acatagcgtc ttgttccgtg ataccaaatg tttcatagaa ttcgtcaagg ttttttggtt
     4081 gcacgtttgc tcggagttta gcaggggcat ggacatcgat ttgcagtaaa agttgttggt
     4141 attctttttt tgcttttgta cgccaaatcg ttgcccaatt gacaaagaat tcctcgatag
     4201 aaagatctga ttccgttttt gctgcttcca atgcacagct tagaccgcct gcatcagcaa
     4261 tattttccga aacggttaat tttccgttta cttttccgtc agcaaaaggc agaccatcga
     4321 attcttcgat cattgcttgt gctttgtttt gaaattgtgt aagatcttct tctgtccacc
     4381 agttgttcaa gttgccatat tcgtcaaata aagcgccgtt attatcaaat gcatgggaaa
     4441 tctcatgagc gataacagct ccgattccac cataattagc agagcttgat tgttctagac
     4501 tgtaaaatgg tgcttgaagg atcgctgccg gaaagacgat cacattccga aatggatgat
     4561 aataagcatt gaccgtgtca gcactcattt cccattctga acgatccact ggtttgttcc
     4621 atttgctgaa acgatctttt aatgtgagtt tgctgaaaac aagagtattt gaaagcaaag
     4681 taccaccctg atctgcaggt actgttttga actggtcgaa taatgctggg atcttatcgg
     4741 ggtagcctac ttggatacct aagttattta attttatgac ggctttttct tttgttgcat
     4801 ccccaagcca gtcattttct tctagacgtt ttttataaac ggcgatcatt tttttcacca
     4861 tttgttctac gtctttttta gcagcttcgc caaaatattt ttttccgtag taatcgccaa
     4921 ctacttgatc gaattgtcca gaggcaaggt aatacgctgc ttttttctga ggcattgctt
     4981 cgtctgttcc agacaatacg cgagaataga ttccgctgac ttggcggaat tcttctgata
     5041 gataaccact aagagaacgt actgttttaa tgatcatcca gcttttgaat aacggaaaat
     5101 gagcttctgt caggatgtca ttcaaatgat cgaagtacac tggatcagtt acgatcactt
     5161 tatctggagt tgtcccaatc aaacccgtga caagtttttt caagtccagt ttatctgtgt
     5221 atttaataaa tgtatcaaaa gattgcggat tatacatttt gctgtaatct gcgttttctt
     5281 ctgcactttt tacatgagga gcaatcaatt tatcaaattg cactgcttgt tccgtgattg
     5341 cttctgcctg acttttttct ttgccggcaa gctctaataa ctgaacagcc atatcaaaga
     5401 aaacgcctat tagttgtgca ccgttgggat ggtctgatgc ataatacgtc ttatctggaa
     5461 ggaataaaga aggcggataa gcaaagaagg cgttcgtttg cgcattcttc atatctgcat
     5521 ctacatccag attaaatggc aatggtaatg tatccaacag ccattctgga aattgcttat
     5581 tcaattcgtc gaaagactgc aatgattcaa ctttttcgat tataggttga agcggttgag
     5641 catgcaactc atttcttcgg tcataatcgt ttgccagttg ataatatgca aggaaatgtt
     5701 tcattttttc tgaagacact ttttctggat gggcaagcag atcgtctgtt tctcccatca
     5761 aaagttggtc gatcccgtca actaaatctt ggaaaccgcc tgtagctggt ttatctgcag
     5821 ggatttttgc tgttttttgc cattcttcat tgactgcttc atagaaatct tgtttcaatc
     5881 gttctttgtt cattcatcga tcgctccttt tttgtttcgt gtgcccatta tcttacaaaa
     5941 tgaaggaatt aacaaataga acataaaaaa ttgaaaattc gaacaatcaa actgatttgt
     6001 ttttttcaat ccttcactta taatgaaaaa aaggaggcga tcccaactga atcaagtagt
     6061 ggataaacct ttatatttca aagtagataa aaaaattaaa aaattagcca gcactcagca
     6121 gctgcagtca agaaaaagta aattgctttt tttagctctg gtttttgaag atcaaagtta
     6181 tgtgattatc gatcaatcag gacatccggt agaatattct ccagcaaaat atacttatca
     6241 agagggtctt tcttgtaaaa aatggaagct gatcaatgaa gagccaatcg agttatctag
     6301 atggatcaac agaaaagaag atattcccgt tttgatcgaa gaaaaaaggt caggaaagga
     6361 actcgcaaat tgctgggtgg gattgccaga agaacgtttt ttgaggtata agaaatgggc
     6421 taccccctca ggctatcttt gtggtactta cgcggcagca gttctccttg cctattatca
     6481 agactatcgt aaggggtgga tgcttccaaa tgagattcgc aagaaaaata cggctgattc
     6541 gttggttttg acgaaggcat tgcgtagcca gatccaacca ctaggacttc ctacgattcc
     6601 ttttcaggtt agtacaggga tttctagttt tttaaagaag aatggcaatc atgagcgtgc
     6661 acgagcaact cttctaggtt catggcagcg agctacaaaa cgtatacgtg aaggcaaacc
     6721 tgtgatgatt ggtattttga aagtacttgg cagtacatat ggcaatcatt gggtgacagc
     6781 gtacgcttat tttgaaactg aaacaggaga acgttattac aaagtacacg acaactgggg
     6841 cgattatcat aaagtcattc ctgccagttg gagtaacgga acagtttcac ttccttaaga
     6901 atttgctgaa attaacagaa aatcttggaa aaatatgtta aactatcgaa gaatagagaa
     6961 gttttgggga ggactacatg agtaataagt atcaggatga cacgttgaag atttttagtt
     7021 tgaatggcaa ccgtccatta gctgaaaaaa tcgcgaaagt gttcggaaca gaattaggta
     7081 aaagcgtcgt gaagcaattt agtgatggcg agatttccat caatatcgaa gaaagtattc
     7141 gaggggatca tgtgtatatc gttcaatcaa cgaatgcacc tgtcaatgat tactatatgg
     7201 aactactgat catgatcgat gcaatgaagc gagcaagtgc gaaaacgatc aatgtcgtat
     7261 tgccttacta tgggtatgct aggcaagacc gcacggcgaa acctcatgaa ccgatcactg
     7321 cgaaattgat cgctaacttg atcgaagaag caggggcaac tcgagtactg actttagatc
     7381 tgcacacggt tcaagtacaa ggattctttg atattcctgt agataattta tttacgatgc
     7441 cgttgtttgc tcattattac cgtcaattag gtctgacggg agatgacatc gtagttgttt
     7501 cgccaaaaaa cagcggtgtt cagcgtgcac gtagtctttc agaatatttg aacagcacat
     7561 tagccatcgt agatcatgca gatgatgctt caactgaggg tggttatgtg atcggtgatg
     7621 ttcaaggaaa gacatgtatc atggtagatg atatcttgaa cacaggagca acgcttgcac
     7681 gagcagcaaa tgtattaaaa gaaaacggcg caaaagaagt atatgcctgt gcgtcacatg
     7741 gattattgtc cgatcatgcg aaaagtattt tagacaatgc accaatcaaa gatatttgta
     7801 tcactgattc tgttttcaca gaagaaaatc gtcatccaga aaacttaaca atcgttacat
     7861 gttcagaatt aatgggtgaa gcggtcaaac gcatccatga aaatacaccg atgagtccgt
     7921 tattccgtct ggaagaaaaa gaattcgaat aaacaaaaaa aagagggaaa gaagttgtga
     7981 cagaagtgag cagcttcaag gagctgcgac aagacttttg tcacagctcc ttttttctat
     8041 gcccatgaaa cgtcctctag acaaaaatga gctcttcttt ggtttgttcg ctattttatg
     8101 ctagaatgtg cctagcaata atttggaagg gatgaatgaa gtgaaaacta tttatctgga
     8161 tcatgcggca actaccccca tgcatccaca agtaattgaa gcgatgacgg caatcatgca
     8221 agacacgttt ggaaatcctt caagcataca tggatttggt cgccacgcgc atgaaaaatt
     8281 ggaaacagca agacaaatca ttgcagacag tctgcaagca aaacctcacg agatcatctt
     8341 taacagtggg ggaacagaag gggataatac agctatctta gaaacagcgt tctcgcgtca
     8401 aaaagaaggc agacatctaa tcactacagc tatcgaacat cctgctgttc ttaataccat
     8461 gaaatattta gagacaaagg ggtttgacgt tacatatctg cctgtagatg aaaaagggaa
     8521 tctgtcgatc gaagaggtca aaaaagcgtt acgagaagat acgatcttgg tctctgtgat
     8581 gtacgggaac aatgaaatcg gaaatttgat gccaatcaaa gaaatcggtg aacttttagc
     8641 ggatcatcca gcttatttcc atacagatgc ggtacaagcg tacggaaacc aaacgatcca
     8701 tccagaagaa ttaggaatcg atttattaag tatctctgct cataaaatca atgggccaaa
     8761 aggcgtcggt ttcttatata aacgagacgg agtaaatatc cctgcattgc ttttaggcgg
     8821 ggagcaggaa gaaaagcggc gtgcaggtac agaaaattta gcagggatct ggggtatggc
     8881 aaaagcagtt gaactgctgt cacctgaaga acgtgaagca cgtaacaaca aatataaagg
     8941 attccagcaa ttgattcttg atacattaga agcaaacgaa atcccttatc aagtaaatgg
     9001 tgatttaacg aataaactcc ctcatgtgtt gaatcttcgc ttaattggcg aagcaaatga
     9061 tttgctactg atgaagcttg atcttaatgg ggtcgctatt tctactggtt cagcttgtac
     9121 agcaggaaat atcgagcctt cacacgtatt agaagcaatg tatggagaag atacaccgat
     9181 actaaaagaa tccatccgta tcagctttgg tttgggaaat accgaagacg acatccagta
     9241 tttccttgat caattagttc gtgctgtcaa aaaataacta gtgaagtcat tcttatatga
     9301 acagatgtct gagagaacgc cttcattttt tagggaactt aactgaaaaa cctagtaaaa
     9361 atcaggataa acttttataa taaagagaaa ctaattttat cgaggtgaaa atcatggcat
     9421 ttgaaacaac agcaacggta ctgggcgcac caattagcta tcgtttgcat cctgatgcaa
     9481 aaagatatac attgcgcgat aatggattta cagaaacaaa cggaggcaat tatcaattga
     9541 tccgaccatt agatgccacg ccgcaaagca aagaaggatt caaattaaaa atcactgtat
     9601 caaaagatat tcaaacattg aaaatgtcga tcactacagc aaatggattg aaagcagtca
     9661 atattttcaa agatccaaaa cagaaaatga gccaagataa attttatttt ctgatggatg
     9721 ggatgattag ccgtgggtta ttcgaaaaag tcgaatgatc tgtggatata aaaagacaac
     9781 tagttttgaa acggcctatt gttagatgac taactttgta aggacgacgc aaattagttg
     9841 tcttttttat ttttaaattt aacacatgtt gttattttta atattttgct gatttattgt
     9901 ttctaagtaa agaaatttaa attgaaacga ttgttattta gggaaaaagg tattttttga
     9961 aaaaagaatc ccgtataatg attgttgtgc aaacgctata caaaatgaaa aagaaaagag
    10021 ggatgtttat ggaaaatgaa cagtcggtcg ttttaacgaa ttggaagaga aattatttgt
    10081 tctttttatc gggacagttt ttgtcaggaa tcactagtat ggtcgtccaa tatgcgatca
    10141 tctggtattt gacaagagaa accggttcgg cgacaatttt gagttttgct actttactag
    10201 gaatgattcc catggtttta ctaagcccat ttgtaggacc gctagtggat cgctgggata
    10261 aaaaagctct tttgattgtc acggatatta ttgtagcgat tttcgcgctt attttagcag
    10321 tagtcggaac gatttcagaa tccttcccga tttggctagt ttttgtgtct ttgtttatgc
    10381 gttcggtcgc acaaacattc caaatgccaa cgatccagtc gattatgccg acaatcgttc
    10441 catcttctca cataacaagg acgaatggac agttaggaat ggtccaatca gcgaatttca
    10501 tcattgcgcc agctctaggt gcggctttgt tttcagtcgt tcctgtcaat taccttattt
    10561 tattagatgt attgggggca gtttttggtg tcggcttatt gatttttgtg aagatcccaa
    10621 aagtttctcc tgaaatattg gaagttccgc ttactatttt taaagatgca aaatttgggc
    10681 tgcagcaatt gatggataat aaagggttat ggtacataac gatcaatggt gcatttgtga
    10741 tgctgttgtt catgccagca atcagtctgt atccattgat gacgctagat tactttggtg
    10801 gttccgttgg acaggcggga gcagtggaag tagtctacgc agtcggaatg ctcttaggcg
    10861 gtgcactgat cagttttata ggaacatgga aagaccgaat gaaacctatt attatagcct
    10921 acatcattat gggcctgacg atcggtgcaa gcggattggt tccaaacgat agtcaagggt
    10981 tcttgtactt tcttatcttg aatgcaggag caggttgtgc aacgccatat ttcaatacac
    11041 tgctgatggc aatgatccag cagagttatg aatcgaatgt tttaggtcgt gtcttaggga
    11101 acttcaactc tttgatgaac ctggcaggtc caattggatt attatttgca ggaccattag
    11161 ctgatcgttt aggggtagaa aaaatgttct tgttttccgg aatcgggatt ttattatgcg
    11221 gaatcgtttt atttttaacg tcagctgccc gaaattatga taaagaatta cagaaaaaac
    11281 tggtaaaaga acaccatgag caaaaagacg aatagtatgg aatggagttg tgacagaagt
    11341 cttgttacaa ctccatttct gatcctaatg ttcctgtggc aataagccta ctcaaatgaa
    11401 aaatatgagt aattatcttt atttgtttaa tacttgatat agggcagttt tttcacgacc
    11461 tatcgtactt cctctttgct tcatagattt ctttgatttg ttgctcagat aacggagtgc
    11521 caaatggtag tttttccttt aagagttctc tttccggtgc gtctacgcct acattgacat
    11581 cgttctcgat aggtacgctg taatgatgga tatgtccatg aagattgcgg atagtgtcgt
    11641 taggcccgag caacatcgga taatgagtca agtaatattg ttgatggtcg aatttaacga
    11701 tggcacctac atcataaaaa taaaatttgg gacttccgtc agtaagccta ggatcatttt
    11761 tttctaaata acggaaaaaa gcgcgactgt catggttccc tttgatcaag tggattgttc
    11821 cttgcagttg agtgaccagt tccaaaattt ctgcatttgc tttttcatat tgcggatgca
    11881 tagcaaggtc gcctagatga taaacatggt cggtttcttt tattactgca ttccagctag
    11941 tgatcaactg ttggttcatt tcatccacat ttgcaaagag ccgcggagca aagtcgtttt
    12001 ttcctaataa gcgttcatgg aaaaagtgca tatcactgat aaagtaattc atgtatcttt
    12061 tgcctccctt ttttcttaga gtaaacaaaa tcacttatct gagcaactca taacatctaa
    12121 aaagaaaagg ttgtaaatga gacgtgcagc tttaagcgat aagaagcaat tttgaaaaat
    12181 agtttttcac aatttataaa attattcctt attgccgaaa ggctggccct ttttatgagt
    12241 atgccaattt tttatatcga atgctcaaaa gatgagtgat tttttggttg actatcatgt
    12301 ttgtgtactg aataggtcca gtagaacagt atttttacga cttgataatt aagggatgaa
    12361 attttcagaa atatgacgta taatgtaaaa tactgaaaag ttgcgacctt caactcgtag
    12421 atgtttcaga atctaattga aatgatggtg atatcatgac agacaacagc aaaacacgcg
    12481 ttgtcgtagg gatgagcggc ggtgtcgact catctgtaac agcacttttg ttaaaggaac
    12541 aaggatacga cgtgatcgga atattcatga aaaattggga tgacacggac gaaaacggtg
    12601 tatgcacggc cacggaagat tataaggatg tagcgaaagt agctgctcaa atcgggattc
    12661 cttattactc tgtgaatttt gaaaaagaat attgggatcg tgtatttgaa tatttcctag
    12721 ccgaataccg cgcaggacgt acacctaacc cagacgtgat gtgcaacaaa gaaatcaaat
    12781 tcaaagcatt cttggattat gcaatggatc ttggcgccga ttatgtagct actggacatt
    12841 atgcgcaagt cactcgcgat gaaaacggcg tggtccacat gcttcgtgga gtagacaata
    12901 ataaagatca aacttacttc cttagccaat tatcacaaga acagctttca aaaacgatgt
    12961 tccctctagg cggcatggaa aaatcagaag tacgggcaat tgctgaacgc gcaggccttg
    13021 cgactgctaa gaaaaaagat tcaacgggaa tctgctttat tggtgaaaag aacttcaaac
    13081 aatttttgag caactatctg ccagcaaaaa aaggaaatat ggtcactctt gatggtgaag
    13141 tcaaaggaca acatgctggc ttgatgtatt atacgattgg tcaacgtcaa ggactaggga
    13201 tcggtggcgg aggagattcg caagaaccct ggtttgttgt aggaaaagat ctagcgacaa
    13261 acacattata tgtggggcaa gggttccatc atcctgcatt gtatgccaca agcttggatg
    13321 caagtgaaat ccactttacg acgaatgaac caatgccaaa agaattcaag tgtacagcga
    13381 aattccgtta tcgtcagcaa gacgttcctg tgacagttcg cttattggat gataatcgtg
    13441 cagaagtcgt attcgacgaa cctgtacgtg caatcactcc tggacaagca gttgtatttt
    13501 atgatggaat ggaatgtctg ggtggtggtt tgatcgacca cgcatatcaa gaaacaaaag
    13561 ttttacaata cgtttaagcg atattaaatg aatcaaaaca gctatggcta acaaaagaaa
    13621 caccttttct tttggatagc atagctgttt ttttgcaata aaattaattt ttattcattt
    13681 tatacttgaa atacttgtgt aaaaagttca taatatccaa taatatgaat aaagttgctt
    13741 tttgttaagc aagagcagtt gcaaaataga agggaatctt ttgcaagtga tttgttcaat
    13801 tttataagtg agggtgatgg aaatggaaaa gaaatcgacg ggtattagca aatggggact
    13861 ggttgctctg gttgtcagtt cgtcaatcgg aagcggtgtg tttgggatca caagtgatct
    13921 ggctagttct gcagctccag ggccagcgat cctttcatgg ataatcgttg ggattggtat
    13981 tttagctttg gtattatcgc tgaatcattt aggagaaaag cgtccagatc tagatggtgg
    14041 tattttcggt tacgcggaag cttcatttgg taaattaggt ggcttcatca gtggttgggg
    14101 ttactggttg tctgcgtggc tgggaaatgt tgcttttgca acgatgctga tgagcgccat
    14161 gggagaattt ttcccgatat tcaaaggtgg acaaaatgtt ccttctattt tatttgcaag
    14221 tatttttatt tggggtctga ctttattagt aaacaatgga gttgaaggag cgagcgtcat
    14281 caatacaatc gtgacgatct gtaaattagt gccattattc ctatttttaa tatttgtatt
    14341 tatcgcattt aaagtgaata tattcactgc tgatttttgg ggtaacgtat ctgataatct
    14401 agtgaatgga acagggcagc aaggaacgat ctggttgcag attaaaggtt gtttgatggt
    14461 tatgatgtgg gtctttgtag ggatcgaagg cgcaagtgtt ttagccaacc gcgcgaagaa
    14521 acgttcagaa gcccagcaag catcgatcat tggtctgctt tgtttattgt ttatctatat
    14581 ccttgcttcg atcttgccat acggcgtctt atctcaagaa gagttagcaa ctatttcaca
    14641 acctgctatg gcaaatattt taaaaggaat cgtgggaaat tggggagcag cgctgatcaa
    14701 tatcggcttg attatttcga tcattggttc ttggttgtct tggacgatgc tcccagcaga
    14761 aacaacgatg ttgatggctc gagacggtct gttgccgaaa ggttggggaa aaacgaataa
    14821 aaaaaatgcc ccaacttatt cgttagttgt tactgcaatc ttgactaatc tattcttatt
    14881 gactttctta gttactgatt atgcctatca atttgcctat tctttatgta cagcggcaat
    14941 tttgatttgt tatctacttg tcgggatcta tcaagtccag ttttctttaa aagaaggaat
    15001 caaaaaacaa gtagtgatcg gtgggattgc tgtagttttt gaattgatgg gcatcatttt
    15061 agcaggattt tcttatgtcc ttttatgtag tattgcctat cttcctggtt tttgcttcta
    15121 ctggatcgct tgtcgtgaaa ccaaacatca aattacggga aaagaaaaag taatgattgg
    15181 gctgatttgt gtggccgcac tgctttcaat cgggttgctt gctgtaggct ggatcaagat
    15241 ataaggggga aagaaacatg aaaatcgcaa atttcggtgt agaagaatgg ctaaataaat
    15301 gggaaaagaa agcggtatat gatattgcac aaagctcgat cgaagcattg acattagacg
    15361 aactcgttgg cttagatggg actagcgtaa gtaaattttt cgaaaaacgc aaaaacgaac
    15421 cattggacta tggctggatc gaaggatcag atgttttcaa acaagaagtc gcttctcttt
    15481 atcaaaccgt cgatccggaa aacatcttac aaacgaatgg agcgactggt gctaatcttt
    15541 tagctctata cgcgttagtg gaagcaggag accatgttgt ttcgatgctt cctacttatc
    15601 agcaattata tgacatcccc aaatctttag gtgccgctgt tgactttgtt catttgaagg
    15661 aagaagaaga ttggcagttt gatttagaac agctagaagc cttagtcaaa cctgaaacaa
    15721 aaatcatctg tttgaatagt gcaaacaatc ctacaggcac attgctagat cggaaaactt
    15781 tagaaaaaat cgcagaaatt gctcgtacag tagatgcata tgtattgata gatgaagtat
    15841 atgcgccttt aacggataaa ggtgaattct tgtcgatcgt agatgtctat gaaaaaggta
    15901 tagcaaccaa ttccctatcc aaaacctact ccattccagg tattcgtatt ggctggacag
    15961 ctactggacc tgagttggca gaagtttttc gaaagtatcg agactacacg atgatttgcg
    16021 gcggagtcct gtcagatgat cttgctgtac atgcattgaa gaataaagaa aaaatattgg
    16081 cgcgaaacaa aaagatcatt acagagaacc ttgtgatttt aaaacaatgg gtagccaatg
    16141 aaccgaaagt tgaacttgtc gctcccaatt acgtctctac ttctttcatt aaattagcca
    16201 tcgaagaaga tgaccagaca ttttgtatca atttattaga agaaacagga gtactattag
    16261 taccaggaag tgcctttgat ttaccgaagc atgcccgact aggttactgt tgtaaaaaag
    16321 aaaccttgga aaagggatta ttcctcttat ctgagttctt gaaaaaacag gcctaaacaa
    16381 aaacactgca aggacagtct gtgacaactg ttcaagcagt gtttttcatt tacgcttatt
    16441 gtctatgaga aggagagtgc cctgaagcta tggagactcc tgaagctttt tcttctgcag
    16501 tttcttgctg atggatctct tgtgtattgt gtcgtacaag caagaagttg accaaaacaa
    16561 gaagagaagt cgaaagaaag acgccgcgat aaccaaatgc actggaaaca ctggagccga
    16621 tcataggccc tactacgtta ccagttgctt ggaaagattg gttataactg aagatccgtc
    16681 cagcagcttg atgaggcgaa tattttgtga tcaatgcttg gacagcaggg agcagacaag
    16741 catcagagat accaatcaaa aaacgtaagg cagctaattg ccaaacattc gtgacgaatg
    16801 ccattgggat ataaaccaga atcgcaaaac ccaaaccaat cgcaagtatt ctttcactcc
    16861 caatcttgtc acctaagcga ccaaatcttg gagcggctat caaagtggca atcccaggta
    16921 tcgaagcaat gattccgctg atcaacgtaa cattcccatg tccatgcagc aattggcgaa
    16981 tgtacagact gatgattgga ctaatggaat tgttggatgc ttggatgatc atcgttgtga
    17041 cgaacatacc gatgacgacg tgtggatatt tcaagtcttt gaaaatctgt ttggcagaaa
    17101 ccatgttttc ttttttcact gggacaaatt tttcatggac gaaaaacaga ctcaacaaga
    17161 agacgaaaaa taagatgatc cctgtaatga aaaaggtagg gcgatagcca aacgctgaag
    17221 cagagatccc gccaagaagc ggtccaagta atgttcctgt aacagaacca gttgctaagg
    17281 ttccaagaac ttgaccactt ttttcttttg gcgtcccagt tgcgacaagg gcagtggcat
    17341 tactgatata accagaaaaa atcccttgaa gcagacgcaa tgcgactaat tgatacacac
    17401 tagtcacgca acccatcaaa gaaatgacga ttgccattcc taatgaagca cgcaacagca
    17461 tcaattttct tcctttttgg tcggctaatt ttccccacca aggagagata agcgtagtca
    17521 ccaagaaagt agatgaaaaa gtcaatccgc tccaaaagtt caattgtgac gtactataat
    17581 tacctaacgt atctatgtaa agtgacataa aaggcatgac taaactaaat ccgataccag
    17641 ccataaatgt tccaaaccat aagacaaaca agttttgttc ccaagtttct cttttcctaa
    17701 ataatttttc ctttattctt gcagccaatt agactccttc tttctgaaaa ttttcatcta
    17761 cagctcttta ttataaacta caatatgtag tttatcaatg aaagtctctt ttttctcttt
    17821 ctttttcgtg gtaaactgaa agagtgaagg atttatttta tgagaggaaa catggaaatg
    17881 aaaaaaagaa atctgtctca agagaaaatc atcgactgct ttcgagaact agcagaagag
    17941 atggatgttc agcaaataac ctttcagcat ttagccaaac gcttggatat caaggctccc
    18001 tcgctgtata accatttcaa aaatattcgt gatgtcaaaa cagcgctaac agcgaaactt
    18061 ctgcaggaac ttaatgacca gctgcgacgg gctcttgtgg gaaaaagtgg tcaggaagca
    18121 ctgcagatct atgcagaaac ttatcgatcc tttgcttttt caaatcaagc agtgtatgag
    18181 ttactgatca gtgtaccgca taccaatgaa gctgttttat tagatggaat ccatgaaacg
    18241 aatcagatca ttttgcagat atttgaggct tttcaattaa cgaaagaaga aaaagttcat
    18301 cgaagcaggg aacttcgtag tatgatccac ggttatcttt ccttacgttt tttaggatac
    18361 tttacaaaag aaccaacaat cgatccagaa gacagctatt tttggatgat caataacttt
    18421 atcgctactt tgccttaaac aatcagattc atttgtaaca attttaagat gttcattaaa
    18481 tgaccctttt ataagagaaa attcttgcaa ctttcttgaa aagtgactaa gatgagtcta
    18541 gaaaagctga aagaaggagc acgagtatgt atcaagtaat taccatgttc ggggacaatg
    18601 agccttggtg gttttttgaa gactgggaag aagatattga agaagaagaa acattcgagt
    18661 ctttcgaaga agcacgacaa gcgtacgaaa aacaatggaa cgaaatccat caaaattatg
    18721 aatatatcaa tgcaaaaagt aactttctca gcgccttttg gaatgacgga gatgagcgct
    18781 ggtgtgaaga gtgtgacgat gatttacagc aatacaaggg acttgcgttg ttgaaaaatc
    18841 atcagcccat cacagtagaa agcagaaagg aattttatga aacaactaat tctagcggaa
    18901 aaaccaagcg ttgcgaaaga cctaagcaag gtgcttggtg ctaatcaaaa gcacaaaaat
    18961 tattatgaag gacccaaggt gatcgttact tgggcccttg gtcatttatt aggattgaaa
    19021 atgccggaag atctgaataa ggaatggcag agctggcaaa tggacacgtt gccaatgatc
    19081 cctaaaaaac taggaatcaa accacttcct aaaacaggac atcaattaaa agcaatcaag
    19141 cagctggctc agcgaaaaga tgtctcagaa gcagtcattg cgacagatgc cggaagagaa
    19201 ggggaacttg ttgctcgctg gatcttagag tatgtgcatt tcaataaacc agtgaagcgg
    19261 ttatggatct cttctcaaac agataaagca atcaaaactg gattcaagca actaagacct
    19321 gcgaaagaat atgatgcatt gtatgaatct gctcttgccc gggcaaaagc ggactggctg
    19381 gtaggattga atgtgaccag agccttaact gtgaagtacg cggataattt atcagcaggc
    19441 cgtgtgcaga caccgacact agcattagtg agagaccaag aaaagaaaat cgagcagttc
    19501 cgtccacaaa cgtatttcac gatcacgttg caagctggaa aagaacaagc caaaatgctt
    19561 caaaaaaatc aatttgcttt aaaaacacaa gaagaagcac aaaaacttgt tcaattgatc
    19621 agtacaagca aaggaactgt cacagaagtc caagaaaaat taaaaacgga atctgcgcca
    19681 ttgccttatg atttaacaga gatccaaaga gaagccaatc aacgctatgg ctattctgca
    19741 aagaaaacat taagtcttgt acaaagtttg tatgagaccc ataagatcgt tacttatccg
    19801 cgtacagaca gcaaatatct gacaaatgac atgaaaagta cgatgaaaga acgattgcaa
    19861 gcagtcagcg actttgctcc tgaggtcaag cagtatttga aaaacggagc catcgtccaa
    19921 cagacaaaag tattccaaga cagtaaagtg accgatcacc atgcactttt gccaactgaa
    19981 aacagagcac gttatgaaaa attaagtaat gaagagcgaa aaatctatca aatgattgtc
    20041 tctcgttttt taggactgtt tgctcaaccc cataaagtca gccaaacaaa agttacggtt
    20101 gaatttggta aagaacgatt catttttcgg caaaatcgtg tcgtccaagc aggatggaaa
    20161 ggcgaaactg aaactgagac ggaaacagtc aaatgggaaa aaggaatgag aatcaatccc
    20221 gatttcgcca tcaaaaaaga actatctgca ccgcctaaac cattgtcaga agcaagcttg
    20281 cttggtcaaa tggaaaaata cagtctggga accccagcta cgcgtgcaga gattatcgag
    20341 aaactaatca aatctgaact gatggaacgt acccctcaag gactgcaagt atctcctaaa
    20401 ggtaagcaat tattagtatt ggtaaatccc tctttagtaa caccagattt gacagaaagc
    20461 tgggaaaaag accttgaagc tattgctgca ggaaagaaac aagcaggaac cttcttgaat
    20521 ggaatcgaaa aagaaacaaa acgcttggtg aatgagatca aatcaagcaa acaagaatat
    20581 caagatttct cgattaccca aaagaaatgc ccagaatgtg gggccaatct gcgcgagaaa
    20641 aacacacgtg acggcaaaat ctatgtctgc accaattcag attgcaacta ccgcagaaga
    20701 aaagatccaa aagtctctaa tcatcgatgc ccgcaatgtc accggaaaat ggaaatcatc
    20761 gaaggtaaaa acggtgctta tttccgttgt aaatacgatg gaacgacaga aaaattgctt
    20821 gataaaaaag agcagaagaa gaaaatgacc aaacatgaag aacgacgctt gctgaaaaaa
    20881 tatgcacaag cagaagaacc agaagaaagc ccattagcag cagcactaaa agcagcaatg
    20941 aagaagtaat aagatatgaa agaggttgtg atagaagtgg tcagcttcaa gaaataagaa
    21001 ggaatttacg aaaattgctt ctcaaatttt tgtgaaattt cggcttattt ccgaagaagc
    21061 tacttctgga acaccattta ttcggaaaaa aggactgtga caagactttt gtcacagtcc
    21121 tttttcattt aaaaaaatgt ttacttcatt tgacgtatat tattggtgag gcttcaattc
    21181 atttggcttc actttttctg tttctcttat gatataaaca ggacgctgtt tcgtttctaa
    21241 gaagattttc ccaatatatt tcccgatgat tccaagacat aacaattgga ttccaccaat
    21301 gaacaggaag atcgagacca tggaaggcca tcctgatgta ggatcaccaa acaacaatgc
    21361 ccgcaaaata atgaacaaaa ttgcaatacc agaaccgaca caagacaaag cgccgataaa
    21421 tgaagcaatc gttaacggtg catccgagaa gttcacgata ccatcaatcg agtagttgaa
    21481 taatttccag aaggaccaag atgtttcgcc agccacacgt tcgcggtttt cataagaaat
    21541 atattctgtt ttaaatccga cccagctaaa taatcctttt gaaaatcggt tgtattctgt
    21601 taattctagg acggcatcca ccatttgacg agtcatcaag cggaaatcac gagcgccatc
    21661 gaccatttct gtatcgctga ttttattgat caactggtag aattttttcg caaagaaact
    21721 gcgtatcgca ggctcgcctt cacgagtcga acgccgggtg ccgacacaat cgatatcaga
    21781 agtagtgatc cggttgatca tctcaggaag cagttcgggc ggatcttgca gatcaacatc
    21841 catcacggtc actaaatctc ctgtagaagc ttgcaatcca gcgtacagag ccgcttcttt
    21901 cccaaaattt ctggaaaatg agacaaagcg gacataagga taagctgagg ctagcttctt
    21961 gattgctgct aacgtaccgt cttttgatcc gtcgtcaata aacaaatatt cgaactcatg
    22021 ggcacctttg aatttttcaa cctcatcaaa aaaaagagga acagtttttt cttcattaaa
    22081 acatgggaca acaattgata ctttcatcgt ttctatcctt tcaatcgtat gtttatatat
    22141 ggtttacatt caaaatttaa taaaccttaa ataacacctt taaaatttga ttttacgtaa
    22201 aagaacaggt attctttttg taaactaacg tatttagttt accatgtttc ctcggtttat
    22261 caaactttta agaacaattt ttattagttg gcaagtagtt ttggatagag aaaaaggagg
    22321 aagttccagt ctattgacta ggagcaaaca aaatgaaaat acacacacct atcggctttt
    22381 tatggttaga tgcagatgaa tccggattga ctgctgtatc ttttcagcca atttcagaaa
    22441 gcgaatcagc gaatcagcga attttaacgc gcacacaaga acagtttgaa gaatattttg
    22501 cgggaaaaag aaaagaattt gatgttcctt tatctataaa gagaggtacg ccttttcagc
    22561 aaaatgtctg gaaagctttg tcgaccattc cttatggaga aatccgaacg tataaagaag
    22621 tagccgttgc tgttgatagt ccgaaagctg tgcgagcgat tgggcaggca aatcgaacca
    22681 atccattgcc aatcgttatt ccttgtcatc gagtgatcgg gcaaaatgga aaactgacag
    22741 gatatatggg aaacgcaata gatggggttg aaatcaaacg ccaattgttg ttgttagaag
    22801 gatatctgca aaattaaaaa cagttgtgat tcggacattg ttccatgcga gaatcacaac
    22861 tgttttttca tgttcattct aaactttgat cagccaaata aatagtaaag ccatcactgt
    22921 ttggagaagc ccgacagcta atccataaga taagaagcgt gtaccttctt tcatgaattt
    22981 tgcaaagtct aaacgcagac caattgccgc taaagcagtg atctcaaacc agctgctgat
    23041 aaaatgtgcc gattggtcaa taactacagg taaagaaaac aagctgttga ccaaacaaaa
    23101 gatcacaaac cctaatacat accaaggaag cggtaatttt cgttttttct gcgtatcagt
    23161 tggcgtttca aacagagctg gttgtttctt gtgcatgaat ttttcaaaca gatagacaac
    23221 aacaacgagc atcataatac gtagaatctt aaatagcatg gcgaattcga caacagaatt
    23281 gtcaagtaag cttgccccag cgactacttg accaacagat tggagtgtac cacccaaaag
    23341 tgcacttttt tcaatgagat ttccaccaaa caactgtaat ccaagaaaag ggagagtcaa
    23401 catcatcaca gtacccagta gattgactag tgtgatgatc tgtccttttt cttcctcttt
    23461 cgcatggatg gaaggggcga ttgaagcaat agcagaagaa ccacaaacag cgtttcctcc
    23521 agccatcatc aaagaagaag tatcgctgaa ttgcagtttt ttaccaatca agtaagcacc
    23581 gatgatagtg actgacattt gcagtaacac aaaaacaatt ccttttacac caatttgagc
    23641 aatcgtttga gctgtgacag ttgcacctag taaaaaaaca gagaattcta atagtttgct
    23701 ttcagcaaat tttgtgccga ttgctagaac aggctgttta aaaaaagtat ttcctaataa
    23761 aatacccaat aaaatagcaa tagtcgctcc acctaactgt ggcagataaa tagcaagaag
    23821 cctgctgaag gcagcgatgg caaaagaaag cagtaaaccg ggtaagattt cgcggaccgc
    23881 tgaatccttt acagatttta ttggatgtga caatgatttc atttgatcgg ttcctttcgt
    23941 caataaatac taaaagcata ctcgaacata gtagcactaa accagtattt tgacaaaaga
    24001 acaagaggtc gtgacaaaag tcttgtacga cctcttgttt cgaataaacg gtgttccaga
    24061 agtagcttct acggaaataa gccgaaattt cacaaaaatt tgagaagcaa ttttcgtaaa
    24121 ttccttctta tttcttgaag ctgaccactt cttttacgac ctcttggtaa acggtgttca
    24181 aaagcttgca tctgcggtaa taagcccaac cagtacaaaa acatgaacag ctgtttttgt
    24241 ttggccgttc ttattacttg atgcagaaca gctttttcac aacctattga atcgattatt
    24301 aagcgatttt tttagaaaca aattttccat gtttgctatg ttctttaatt aagaaacgta
    24361 cgtaatgagc aagaacgtag aagacaacta cacttgcaaa cagacattcc gcaactttta
    24421 agaaatccaa tacgctacta tagataccta aactgttgaa gtaatgaaca gtagtaaata
    24481 tagctgttgc agagataaca agcgggaatg taaaggctgc atagcttgga taaaaacgaa
    24541 gcttgaacaa acgtggaagc atagacagca caacgaaata caatacttga gataaagcga
    24601 acaatgctac gacaagataa aaattaggtt cagcaaagtt tttaagataa cctgttaggc
    24661 aaagagaacc aggtgctgca acgatcgtaa tcagcggacg tgtcggatga gccatatttc
    24721 ttacgaaaaa gacacgtttg atgatgatcg gcaataagaa cgcatagcag atcaatgaaa
    24781 tccaaaagaa aatttttcca ataagtggga agaagttccc ggcagttaca gtgatgaccc
    24841 caaatccaac gtaaatgata aaccagcttg gataaattgc ttcaattgaa agatcagctt
    24901 tgataacatg gtgataattg aaataaaaga caaggatcac ttggaagata acagcgcaca
    24961 gccaaatgaa tttgacaatt ggtgcaatac tttcatatga aacaaaataa gtacaaataa
    25021 ccatgagtga catcgtaaaa gttggtgaaa cggaggcgat gattggatca tgcaacgttt
    25081 gtttcgtgtg ttcgaataag ataaacaatt taacaaaaat gaatatcata agaatcatag
    25141 agataccgcc agttagattg cctaaagcaa catggtgata gtctttaaac agatttccaa
    25201 gggaagctaa cccaaggatc aacccgcaga tcgggatcgg aataattttc aaaaagttct
    25261 tcatatataa tattctcctt tttatttaat agattttagt accaagatga acgaatacat
    25321 tgtaaaatga ataggaatgt atgattacga gcaagcagac agctgattgt cttcatctaa
    25381 gttactagtt ggatcagcaa ctgttttgtt gtacatttct attatagatg gttttttgta
    25441 tatttaaatc aaatagtttt tatagtaatt atcataaaaa gttatggaag tggtgccatg
    25501 tttaagttat taacgacttt tcgagcagta tatgaaacaa gaaatttttc cagagcagca
    25561 gaagtactgt ttttatctca accagcagtc tcgaatcaaa tcaaacagtt ggagcgggaa
    25621 ttgaatatcg atctttttca acgtaacgga agaaaagaga tgcttccaac taagcaagcc
    25681 gacttattat atgaacggac actggttttg ttagaagaat gggaagaaac tcagcaaaaa
    25741 ttgctcaatg aacaaaatga agaagaagta tgtcggatcg ttgcttccca cacctttgct
    25801 gtctatcttt tgcctcagtt gatgaaacat ctgatcaaaa ggtttcctaa agtgcatttt
    25861 gagatcgaaa tcacgaattc tcatgaatca ttagaacaag tagcgaaaca tgaatccgat
    25921 tttggtttta tcgaaaagcc attagagact tccggcgtac aaagatactc tttgatggaa
    25981 gaccagctag tcatcgctgg ggacccaaaa agcaaattgt ggcttgtaag agaagcaaac
    26041 tctggtgttt ttcattacac gcagcgttat tttgaagaga tgaatatcca agataagaag
    26101 atgatcgtga aaagtaacga tgtaattgtt gcgttgctga aggaaggaat cggtcgctcg
    26161 attttgtcta agcgctctgt tccagaaaat gtgccaatga ctccgttgaa tttccatcga
    26221 tatttttatt tcatccaacg aaccaatctg gtttctgatc agctgctaga gattgccgaa
    26281 gagatccttc gatacaatga aagcacagaa acttctcaca gcgaggagaa tctcagctgg
    26341 tagataattg catgtatcaa aaaaagttct atgatacact aaagataagc agtaggcttt
    26401 tgtactgcat ttgctcaagc aactgcacaa acggaaagtt aatttttccc aaatgtctat
    26461 ggaaagactt tccattatct aactggaccg ctataataat cagcagtatg gtttttaaga
    26521 aaagggatga aacatggaaa aaaaattaaa agcttcgaac gataatcgta ttgattatgg
    26581 cgtgatttta ccagtctttc tgttatgttt gattggtctg ctctcattat atgtagcact
    26641 tagccatgat ccgaacaatc cagcagtctt gaaaggcgta gggatgcaag ttgtctggta
    26701 tttagttgga gcggcagcaa tcgttgtcat catgcatttg aattccaaat ggatctggaa
    26761 actgactcct tacctttatg gtttggggtt agtggtaatg ggactcttgc tcaaattcta
    26821 tgatcagaat ttagctgctt caacagggtc taaaaactgg tttcgattcg gtgcgtttac
    26881 tttccagcca gcagagttga tgaagattgc gtacatcttg atgatggcac tgatcgtaac
    26941 taaacataat acgcaagtaa aagaacggac gatgaaatcg gacttttggc tgattgggaa
    27001 gctcttgatc gtgacaatac cggttttagc attgatcatg gcccaagatg actttggaac
    27061 gatgcttgtc tttcttgcga tctttggcgg gattttcctg atgtctggaa tctcttggcg
    27121 catcatcgca cctgtagtaa tcttggcagt agtcgttgga gcaggaacga tcttcttggt
    27181 tacgactgaa ggcggaagag acttgcttta taaagttgga ttcaaatctt atcagtttgc
    27241 acgtattgat tcatggctgg accctttcca tgatacgtca ggaatgagtt atcagccggc
    27301 ccaaggactt ttggcaattg gtaccggtgg cttgtttggt aaaggattca acgtaagcaa
    27361 tatctatgtg cctgttcgtg agtccgatat gatttttacc gttatcggtg aaaacttcgg
    27421 ctttatcggt ggtgcattcg tcattttctt gtattttatt ttaatctatc gtatgattcg
    27481 ggtgtgtttc gatacgaata atgaatttta cgcctatatc gcaagcggtt tgatcatgat
    27541 gctgctgttc catgtctttg agaatattgg agcaaatatc ggattacttc ctttaacagg
    27601 gatcccattg cctttcatta gtcagggagg atcctcgatt ttagggaaca tgatcggtat
    27661 cgggttgata ttatcgatga gataccaaaa tgaagcacct gcaagatctt caaggaggta
    27721 agaaaattgt acacctttta ctggtacccg aaatgttcaa catgcaaaaa agccaaagcg
    27781 tggttagacc agcatggcgt tagttatcaa accgttgata tgatcaaaga agtaccgaag
    27841 gcctcaacga ttgagacttg gctagaaacg agtcaagtac caatccgtcg cctgttcaac
    27901 acaagcggga tgaaatatcg ggaacttgga ctgaaagatc aagtaggcga gtttacggct
    27961 tcaaaagcga gccaagtctt atcgacagat ggtatgctga tcaagcgacc actgatctta
    28021 cgagaggacc agcttgttgc aattggattc aatgaaaaaa tttatgaagg agaactgata
    28081 taaaatggca ggcaagcatt tgaagaaaaa ggacaacctt tggatcttat ttaatggaaa
    28141 agaatactgt gttggtttga caaatgaagc gcaagaagaa cttggagaca tcacgtttgc
    28201 caatctgcca aaagcaggac aatcgctaaa aaaagatgaa cctctgatcg aagttgaagc
    28261 ggaaaaagcg gtcaatgaat ttgccagccc gctaacgggt acagtttctt ctgttaatga
    28321 taaaatcggt gaagatatca atatcttgaa tgattcagat gaaatgaatg cgtggatctt
    28381 aagtttcaaa gatgttgatc caaaagaatt tgacgaatta taaaatcaga aaatcacttg
    28441 aaaaaagttg acactgattc ttccagttgc tataatcata agcaatccta tatgaaacga
    28501 gaaagctttg aaaagaagag tacatgtcac ggacgtttga agagagccct gtttgctgaa
    28561 aatgggtaac gaaaatgaga tggaagatgg tcttagagca gagtaagcga gcgtaagcaa
    28621 acttactacg ggagtgcccg ttacagcgcg acagtatacg gaaagaagct aagtccaagt
    28681 actggaagag gctgttattg cgaagtaacg gtaaatcaag gtggtaacac ggaagttcaa
    28741 gctttcgtcc ttttgcttaa ggcaagaggg cgaaggcttt tttgtttgaa aaattataag
    28801 agaaagatgt gaatggcatg tcattaatcc aactaacgaa tgtcaaaaag acattcaatg
    28861 ggaaaaacgg aaacatccaa gccgtcaatg acgtttcact aaacgttgaa aaaggggata
    28921 tttatggaat cgtcggctat tctggcgcag gaaaaagtac attagtccgt ctcctgaatg
    28981 gacttgagct tcccacgagc ggcgaagtga tcgtaaacga tcaagatatt acaaaattaa
    29041 aaaacaaaga attacgtaca ttccgaaaga aaatcggtat gatcttccag catttcaacc
    29101 tgttatggtc aagaactgta cttgaaaata tccagctgcc gctggaatta gctggcgtac
    29161 caaagagcaa acgtaaagaa cgggcagaag aactgttgcg tttagtcggg ttagaaggac
    29221 gcggacaagc atatccttca caactttccg gcggacagaa acaacgcgtc ggtatcgcac
    29281 gagcattggc aaatgatcct gagatcctgc tttgtgatga ggctactagc gcattggacc
    29341 cgcaaacaac agaagaagta ttagacttat tgttagcgat caataagaaa ttgaatttga
    29401 ccatcgtctt gatcacacat gaaatgaatg tgatccgcaa aatctgcaac aaagtcgctg
    29461 tgatggagtt ggggaaagtc gtagaagaag gagacgtatt gactgtcttc agacaaccga
    29521 aaaaagaagt aaccaaacgc tttgtacaac aagacatcga accagaagct gattcgacag
    29581 aactattagc tgaactcatc aaagaaaatc caagcggccg tttggcaact ttgaccttta
    29641 gcgaaagaaa tgccaacgag ccagtcattt cccaagcgat tcgacgctat aacatcgatg
    29701 tcaacgttgt gtatggaaag atcaaacaag tgaaagaagg ttcctttggt tcattgactg
    29761 tcttgatgac tggtgaaacc aaagaattag atcaagcaga agtgttcatc aaagaacaag
    29821 gagtaggaat tgaggtgatc catcgtggat aaaagctttt cagaaacata tctagatttt
    29881 aaacaaatca acccggaaac gatgaatcag gctatccttg atacactata tatgacatta
    29941 atctcaatgg tcttagtgac gatcatcgga ttagttttgg gattgatcct ttactctatt
    30001 ggacgcaaaa acaaaccgta tgctcgaatc atttatggaa tcgtatcgat catcagtaat
    30061 attttccggt caacgccatt tatgatcctg atggttttga tcattccatt tactaaagca
    30121 atcgtaggca ctatgctagg tgcgcaagca gccattccag ctcttgtttt atccgcagca
    30181 ccattttatg cccgtctagt agagattgct tttcgtgaag tcagccctgg tgtactggaa
    30241 gcagcagatg caatgggagc aagttactgg gaaatcatct ggaaggtctt gatacctgaa
    30301 agtattcccg cattgatttc tggattaaca gttacaacga tctcgatgat cggatttact
    30361 gcgatggccg gcgcgatcgg cgcaggtgga cttggaggtc ttgcttggca ggaaggctac
    30421 cagcgtggta atctaacagt gacattcgtt gcgactttaa ttattttact aatcgtattt
    30481 atcgttcaag gaatcggtga tttcttaact aagaaaacag acaaaagata ataggatata
    30541 gggggatgta ggatatgaaa aagaaaattt ttggatttgc agcagcgctt ttgctgactg
    30601 taggattagc agcatgcggt aacaacagcg acagcaaaga ttcagcaaat aaagcagacg
    30661 acacgacatt gaaagtcggc gcttcaccga caccgcacgc agagattttg gaacatgtga
    30721 aaccattatt gaaagatgaa ggaatcgatc ttgaaatcgt caaattcgat gactatgtat
    30781 taccgaacca atctttggaa gaaggcgaca ttgatgccaa ttacttccag catattcctt
    30841 atttgaacaa agaaatcaaa gaaaaaggat atgactttgt caatgccggt gctgtccaca
    30901 tcgaaccaat gggcttgtac tctaaaaaag tcaaagatgt ttccgaatta aaagatggtg
    30961 caacaatcat tacttcaaat tctgaatcag actggggacg catcatcaca attctacaag
    31021 atgctggctt agtcaaagtc aaagatggag tagatctgga aacagcaaca ttcgatgata
    31081 ttgaagaaaa ccctaagaac ttgaagttca accatacgat cgacccatca cttttagctt
    31141 ctacgtacca aaatgatgaa ggtgacctag ttgcaatcaa cgcaaacttc gcttacggcg
    31201 cagggttgaa tccattagaa gatgccgtat tgcttgaaaa ggacaactct ccttacgtga
    31261 atattattgc gactcgtaaa ggtgatgaag acagtgacaa aatcaaaaaa ctgattgaag
    31321 tgcttcaccg caaagaagtc caagaatgga tcacagacaa atggggcggc tctgttaaac
    31381 cagtaaaggc tgatgcaaaa taaggtatgg aacaaaaaag atgagaaata agtctgtaat
    31441 ggcttatttc tcatcttttt tatacctatt ctaatttaga aatcaaaatt gtttaataat
    31501 aaataaaaga tatttgattt tagtaaggaa ggtcattgcg cttcattctc aattagtcta
    31561 aaatgagtga gaacgaaaga gaatttgggg gtataatgca tgtctgtctt agaaataaaa
    31621 aatctacatg tgtcgatcga ggaaaaagaa atccttaaag gcgtaaacct aacaatgaaa
    31681 accggagaaa tccatgccat catgggacct aacggcacag gaaaatctac cttatctgcc
    31741 gctatcatgg gcaatccaaa ctatgaagta acagaaggcg aaattttgtt tgacgggaaa
    31801 aatgttttag acttggaagt agatgaacgt gcgcgacttg gcttattttt agctatgcaa
    31861 tatccaagtg agataccagg tatcacgaat gcggaattca tgcgtgcagc gatcaatgcg
    31921 aaacgtgatg aaaacgacaa aatttctgtt atggcatttt tgaaaaagtt agatgaaaaa
    31981 atggctttac taaatatgcc agaagaaatg gctgaacgtt atctgaacga aggtttttct
    32041 ggcggagaaa agaaacggaa tgagatcctg caattgttga tgttagaacc tacatttgca
    32101 attttagacg aaatcgattc aggtctggat atcgatgctt tgaaagtcgt tgctaaagga
    32161 gtaaatgaaa tgcgcggaga caatttcggt gcattgatca tcacccacta tcaacgatta
    32221 ttgaactata tcacacctga tgtcgttcac atcatgatgg aaggccgagt agtcatgaca
    32281 gggaatgctg acctagctaa acgtctggaa gcagaaggct acgctggtat cagtaaagaa
    32341 ttaggaatcg actataaaga agaagaagcg taagggggag caagaatgaa agaaaaaaac
    32401 tggctcgatt atcttgacgc ggtaaatgat ttttctttaa gtaaaggcga acctgattgg
    32461 atgagaacct tccgtcaaga cgcactagcg aaagcagatg aactgccatt gccgcatatc
    32521 gatcgggtga aattccatcg ctggtcgtta tttgatgtga aagaaacaca aaccatcagt
    32581 gaaacaggca cgattccggc atttgatgca atgaaagaca atcctgtgct tgtccaacaa
    32641 ggttcatgga cgatttttga acaactgcct gtggaattag cagaaaaagg agtcattttt
    32701 accgacttgt tcactgcaat gatcgaatat ccagaactag tccaagaata ttatatgaaa
    32761 aaagcagtaa acatgaacga agaccaattg actgcactcc atgtagcttt tatgaacagc
    32821 ggtattttcc tgtatgtgcc taaaaatgtc gtgatcgatg aaccactcga gtccttattc
    32881 attcaagatg gcgcatccga tgagcacttc ttcaagcatg tgttgatcgt ggcagatgaa
    32941 catagtgaat tttcttatct ggaacgtttc cagacaacca aagaacaagt agcaaaatca
    33001 tcaggtaata tcatcgtgga agtcatcgct aaagctggtt caaaaatcaa atattcagcc
    33061 gtagatcaat taggagaaaa tattacttct tatatgaatc gccgtgggca cattttacga
    33121 gatgcttctg tcgactgggc aattggtgtc atgaatgacg gtcatgtgat cgctgacttt
    33181 gactcagatt tagcaggaga aggtgcacac gcagaagtca aaatcgtggc aatcagctcg
    33241 ggcagacaaa tccaaggaat cgatacacga gtaaccaata aagcccctca tacgatcggg
    33301 catattttgc aacacggagt gatccgagaa aaaggaacat tgacctttaa tggtatcggc
    33361 cacatcttga aaggggcaaa aggtgcagat gcccaacaag aaagtcgtgt cttgatgctt
    33421 tctgataaag cacgtggtga tgcgaatcct attcttttga tcgatgaaaa cgaagtaact
    33481 gctggacatg ctgccagtgt aggacgagtt gatccagaag aaatgtacta tctgatgagt
    33541 agaggattgc ataaagaaga agcagagcgc ttagtgattc gaggattcct cggttctgtc
    33601 ttaacagcaa tccctgtaga acaagttcgg aaagaattag tcgaagtaat cgaagggaag
    33661 ttgaatggat gatcgatgct aaaaagctac gcaatgattt tcctatctta tttcaagacg
    33721 tcaatgatga acctttagtt tatctggata atgcagcgac gacccaaaag ccaaaacaag
    33781 tactccaggc attagcgtac tattacgaac acgataacgc caatgttcat cgcggtgtcc
    33841 atacgttagc agaacgcgcg acccatgatt atgaacaagc aagagaaaaa gtacgggcgt
    33901 tcatccatgc aaaagaaaca gcagaagtgc tgtttactcg tggtacgacg acaagcttga
    33961 attgggtagc cagaaccttc ggcgaccgct atatcaaagc aggagacgag atagtgctct
    34021 cttacatgga acatcattct aatgttatcc cgtggcagca gttggcaaaa cgaacaggtg
    34081 ctgtcttgaa atatatcgaa ctaacagaag aaggcttcct ggatatggag gatgcagcga
    34141 aaaagattac ggacaaaacc aaaatcgtct ccattgctca tgtatccaat gtcttaggtg
    34201 tcttcaatcc tgttcaagaa ttagctgaac ttgtccatga aaacggcggg gtcatcgtag
    34261 tagatggtgc tcaatctgcc ccgcacatgg ccattgatgt ccaagcacta gactgtgatt
    34321 tctttgcttt cagcggacac aaaatgtgcg gaccaacagg tattggtgtt ttatatggta
    34381 aacgaaaatg gttagaagaa atggaacctg ttgaatttgg tggcgaaatg atcgattttg
    34441 tcgaattata cgacagtaca tggaaagaac taccttggaa attcgaagca gggacaccaa
    34501 atatcgccgg cgctatcgca ttaggccatg caatcgatta tttgcaaaaa ctcggtatgg
    34561 acaatatcca tcgatatgaa gaagaactag ccgcttatgt gttgccaaaa ttacaagcaa
    34621 ttgatggatt gactgtttat ggtccacaag acccgaattt tcggacagga gtcgttgcat
    34681 tcaatttgga tgggttacat ccccatgatg tcgcgacagc tttagacatg gaaggggtag
    34741 ccgtcagagc aggtcatcat tgtgcgcaac cgctattgaa gtaccttgaa gtatcagcga
    34801 ctgctcgcgc gagcttttat ttctacaaca caacgcaaga cgccgaccgt ttagttgaag
    34861 caatcatggc aacaaaggag tttttccaac atgggactat ctaaattaga caatttatac
    34921 cgtcaagtga tcttggatca ttcttcccat ccgcatcatc gtggaagctt gactgactcg
    34981 acaagcaaga tcgaattgaa caaccccaca tgtggagatg tgatcgaatt gcaagtcgat
    35041 gtaaaagacg gaatcatcaa aaatatcgcc tttgacggca gtggctgctc catcagtacg
    35101 gcaagtgcct cgatgatgac agacgccgtc atcggaaaat caatagaaga agcagaacat
    35161 ttaactgtag actttctaca gcttgtccaa ggaaaagaag tcaaagatcc cgaccaatta
    35221 ggcgatgctg ctatgcttgg tggtgtagct aaatttcccg ctcggatcaa atgtgcgaca
    35281 cttgcctgga aagcactagg aaaagcaatg gaaaacgatg gtaccgcagc tttccatcat
    35341 taccacaagg aagaggagtg agaacatggg cgtaccagaa ttagaagaat ataaattcgg
    35401 gtttcacgat gatgtcgaac cggttttcag tacaggagaa ggcttgacag aagaagtgat
    35461 ccgtgagatg tcaagaatca aaggcgaacc agaatggatg cttgaattcc gtctgaaatc
    35521 tctagaaaca tttaacaaaa tgccaatgca aacatggggc cccgaccttt cagacatcga
    35581 tttcgatgcc atcaaatatt accaaaaacc aagtgacaaa cccgctcgtg actgggaaga
    35641 cgtaccagaa aaaatcaaag atacatttga aaagattggt ataccagaag ctgaacgagc
    35701 ttatctagcc ggtgcttccg cacaatatga atcagaagtc gtgtaccata atatgaaaga
    35761 tgaattcgaa aagttaggaa tcgtctttac tgacactgac tctgctttga aagaataccc
    35821 tgaattattc aaagagtact tttcaaaact tgtaccgcca accgacaaca aattagcagc
    35881 gctgaattca gccgtatggt caggtgggac ctttatttac gtgccaaaag gcgtcaaagt
    35941 cgatgttcca ttacaaacgt acttccggat caatgcggaa aacacggggc aattcgaacg
    36001 tacgctgatc attgttgacg aaggagcaag catccactat gtcgaaggtt gtacggcacc
    36061 tacttattca agtaacagcc tgcatgccgc catcgtagaa atctttacaa gaaaagatgc
    36121 gtattgccgt tacacaacga tccaaaactg gtcagacaat gtgtacaact tagtaaccaa
    36181 acgagcaaaa gcatacgaag gcgcaaccgt tgaatggatc gacggaaacc taggtgcaaa
    36241 aacaacgatg aaatacccaa gtgtttactt agatggaaaa ggtgcacgag gcaccatgct
    36301 ctccatcgcc tttgcaggag caaaccaaat ccaagatacg ggtgcaaaaa tgatccacaa
    36361 tgcaccaaat acatctagct ctatcgtctc taaatcaatt tcaaaagacg gcggagaagt
    36421 aaactatcga ggacaagtaa cctttgccaa aaacagtgcg ggttcgatct ctcatatcga
    36481 atgtgacacg atcatcatgg acgaatggtc gaaatccgat acgatcccat tcaatgagat
    36541 ccacaacagc caagtctctt tagaacacga agccaaagtc tcaaaaatct cagaagaaca
    36601 actctactat ctgatgagcc gaggcctgac cgaatctgaa gcaactgaaa tgatcgtcat
    36661 gggctttgtt gaaccattca ccaaagaact tccaatggaa tatgcagtcg aactcaatcg
    36721 attgatcagt tatgagatgg aagggtctgt aggttaataa ctatttcatt gctcgaaacg
    36781 ttgatattaa agcgtttcga gcgttttttt atttgtattt taatggttgg ctacgttttt
    36841 ggctacgttt ggaattattc gcccataaat tttgcaaatc ggtcaccagt tttcttaaca
    36901 gattgtggtg tgacatgagc atagatattc atggtagttt taatatcttt atgacctaat
    36961 cgttcctgta cttcttttat cgacgcacca ctttcaaaca ataagctaca gtgggtatgc
    37021 ctaaatccat gtggagtaat ttgaggctcc atcggtgtct ttttgtaaat ccagtctaac
    37081 caatcgttta ctacttgggg gtagtacaac ttgtttgtgt ttgtagtgaa aagaaactgt
    37141 tttggtgaat tcgtgttata gccatataag atcattattt tcatttgttc ttgttgccaa
    37201 atacgcagtt gtttaagtgt aaaatcatca agctgaatcg ttcttgatga atttttagtt
    37261 ttaggtactt gtagaactac ttgattaaac tcgtcttgag cgatagtttt accaattgtt
    37321 attgttttat tgaaatagtc tatatcttcc cattgaagcg ccaatgcttc gcttttacgc
    37381 attcctgtat aagctaataa acggaaaaaa gtgtacagtt taactgaatt attttcttta
    37441 gtattctgta aaaacagttg taattgatct ttcgtgtaaa acttcaaggg ttgttcttct
    37501 tcaatttttc ttggaagcaa tgttttagac ataggattat ccttacagac ttccattgct
    37561 actccaaatt taaatatttg agcagttgtt tttctcaaga aaccatattg ttttaatgtt
    37621 ttgtgccatt cattcacctg tttttggcaa tagctaacac ttattttatc caatcgtaat
    37681 tccccaaaag caggtaatat atggtttttg caaaaacgtt gagcaacggc aacagaagac
    37741 ggttttactc ttgttctgta gttatcaatc cataattcat acaactcttg aaaagttgta
    37801 acattgattt taaactcttg tttttcttta aagtctacaa gtagtctatt aagtgctgtt
    37861 tgcgcctcag cttttgattt gaatcttcta cgctttgtac gtatatcttt tccagtcttg
    37921 ggatctttcc ctaaataagt ttcaaatccc ca
//
LOCUS       DAZUKC010000020        37760 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000020 DAZUKC010000000
VERSION     DAZUKC010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.020"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(101..976)
                     /locus_tag="ACG80T_000952"
     CDS             complement(101..976)
                     /locus_tag="ACG80T_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4840621.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            complement(1085..2854)
                     /gene="aspS"
                     /locus_tag="ACG80T_000953"
     CDS             complement(1085..2854)
                     /gene="aspS"
                     /locus_tag="ACG80T_000953"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFS4840622.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            complement(2869..4161)
                     /gene="hisS"
                     /locus_tag="ACG80T_000954"
     CDS             complement(2869..4161)
                     /gene="hisS"
                     /locus_tag="ACG80T_000954"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFS4840623.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            4555..5502
                     /locus_tag="ACG80T_000955"
     CDS             4555..5502
                     /locus_tag="ACG80T_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS4840624.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            complement(5591..6850)
                     /locus_tag="ACG80T_000956"
     CDS             complement(5591..6850)
                     /locus_tag="ACG80T_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFS4840625.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(7021..7467)
                     /gene="dtd"
                     /locus_tag="ACG80T_000957"
     CDS             complement(7021..7467)
                     /gene="dtd"
                     /locus_tag="ACG80T_000957"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFS4840626.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            complement(7478..9691)
                     /locus_tag="ACG80T_000958"
     CDS             complement(7478..9691)
                     /locus_tag="ACG80T_000958"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFS4840627.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            complement(9924..10688)
                     /locus_tag="ACG80T_000959"
     CDS             complement(9924..10688)
                     /locus_tag="ACG80T_000959"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFS4840628.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            complement(10689..11636)
                     /gene="prmA"
                     /locus_tag="ACG80T_000960"
     CDS             complement(10689..11636)
                     /gene="prmA"
                     /locus_tag="ACG80T_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFS4840629.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            complement(11649..12137)
                     /locus_tag="ACG80T_000961"
     CDS             complement(11649..12137)
                     /locus_tag="ACG80T_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFS4840630.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            complement(12103..12774)
                     /locus_tag="ACG80T_000962"
     CDS             complement(12103..12774)
                     /locus_tag="ACG80T_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFS4840631.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            12912..14198
                     /locus_tag="ACG80T_000963"
     CDS             12912..14198
                     /locus_tag="ACG80T_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFS4840632.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            14270..14464
                     /gene="ssrS"
                     /locus_tag="ACG80T_000964"
     ncRNA           14270..14464
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACG80T_000964"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            14542..14826
                     /locus_tag="ACG80T_000965"
     CDS             14542..14826
                     /locus_tag="ACG80T_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840633.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            14899..15378
                     /locus_tag="ACG80T_000966"
     CDS             14899..15378
                     /locus_tag="ACG80T_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS4840634.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            15600..15743
                     /locus_tag="ACG80T_000967"
     CDS             15600..15743
                     /locus_tag="ACG80T_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFS4840635.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            16088..16216
                     /locus_tag="ACG80T_000968"
     CDS             16088..16216
                     /locus_tag="ACG80T_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840636.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            16321..17904
                     /gene="abc-f"
                     /locus_tag="ACG80T_000969"
     CDS             16321..17904
                     /gene="abc-f"
                     /locus_tag="ACG80T_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFS4840637.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(18341..19525)
                     /locus_tag="ACG80T_000970"
     CDS             complement(18341..19525)
                     /locus_tag="ACG80T_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFS4840638.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(19550..20557)
                     /locus_tag="ACG80T_000971"
     CDS             complement(19550..20557)
                     /locus_tag="ACG80T_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4840639.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(20729..21088)
                     /gene="comGG"
                     /locus_tag="ACG80T_000972"
     CDS             complement(20729..21088)
                     /gene="comGG"
                     /locus_tag="ACG80T_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFS4840640.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(21060..21485)
                     /gene="comGF"
                     /locus_tag="ACG80T_000973"
     CDS             complement(21060..21485)
                     /gene="comGF"
                     /locus_tag="ACG80T_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFS4840641.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            complement(21448..21795)
                     /locus_tag="ACG80T_000974"
     CDS             complement(21448..21795)
                     /locus_tag="ACG80T_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840642.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            complement(21767..22237)
                     /gene="comGD"
                     /locus_tag="ACG80T_000975"
     CDS             complement(21767..22237)
                     /gene="comGD"
                     /locus_tag="ACG80T_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFS4840643.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(22212..22490)
                     /gene="comGC"
                     /locus_tag="ACG80T_000976"
     CDS             complement(22212..22490)
                     /gene="comGC"
                     /locus_tag="ACG80T_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFS4840644.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(22487..23536)
                     /gene="comGB"
                     /locus_tag="ACG80T_000977"
     CDS             complement(22487..23536)
                     /gene="comGB"
                     /locus_tag="ACG80T_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFS4840645.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(23493..24422)
                     /gene="comGA"
                     /locus_tag="ACG80T_000978"
     CDS             complement(23493..24422)
                     /gene="comGA"
                     /locus_tag="ACG80T_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFS4840646.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            24554..26308
                     /gene="ade"
                     /locus_tag="ACG80T_000979"
     CDS             24554..26308
                     /gene="ade"
                     /locus_tag="ACG80T_000979"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFS4840647.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(26406..27068)
                     /gene="trmB"
                     /locus_tag="ACG80T_000980"
     CDS             complement(26406..27068)
                     /gene="trmB"
                     /locus_tag="ACG80T_000980"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFS4840648.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(27138..27920)
                     /locus_tag="ACG80T_000981"
     CDS             complement(27138..27920)
                     /locus_tag="ACG80T_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFS4840649.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(28124..29335)
                     /locus_tag="ACG80T_000982"
     CDS             complement(28124..29335)
                     /locus_tag="ACG80T_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4840650.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(29328..30065)
                     /locus_tag="ACG80T_000983"
     CDS             complement(29328..30065)
                     /locus_tag="ACG80T_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4840651.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            30203..30637
                     /locus_tag="ACG80T_000984"
     CDS             30203..30637
                     /locus_tag="ACG80T_000984"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFS4840652.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            30630..30974
                     /locus_tag="ACG80T_000985"
     CDS             30630..30974
                     /locus_tag="ACG80T_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFS4840653.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            31154..32164
                     /locus_tag="ACG80T_000986"
     CDS             31154..32164
                     /locus_tag="ACG80T_000986"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4840654.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(32233..33177)
                     /locus_tag="ACG80T_000987"
     CDS             complement(32233..33177)
                     /locus_tag="ACG80T_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS4840655.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(33161..35881)
                     /locus_tag="ACG80T_000988"
     CDS             complement(33161..35881)
                     /locus_tag="ACG80T_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4840656.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(35878..37098)
                     /locus_tag="ACG80T_000989"
     CDS             complement(35878..37098)
                     /locus_tag="ACG80T_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFS4840657.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(37379..>37760)
                     /locus_tag="ACG80T_000990"
     CDS             complement(37379..>37760)
                     /locus_tag="ACG80T_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HFS4840658.1"
                     /translation="IEKDAISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNI
                     EIVPQNVSKGVVFDRLESIYNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMK
                     KADYVTVSNDKDGVSQAFERILTI"
BASE COUNT        11132 a         7845 c         6639 g        12144 t
ORIGIN      
        1 aagcagtttt tctttgctga ttctactatg gtagaaaaaa actgcagaca aacgaacgtc
       61 tgtctacagc agtatttcat gaatttttgt caaactcgat ctaaatgcca tcctcataga
      121 aacgaccaag tatctggacg ttttctgcaa tacttacaaa agcttgtgga tcagaagctt
      181 tcattgcttc tctaagtaga ggcagttcgt atcgtgtaac gatcgtcatc aatactgtct
      241 gcttatcatg cttataagca ccttctgctt cattgatgat cgtgatccct ctacgcatac
      301 agttttggat ttcatttact actttttctg gatgtttcgt tacgatcatt acctgcatct
      361 tcttttgttt agtataaacg acatcagtta cttttccact gacgaaaata gacaacgtac
      421 tgtaaaacat atattgccag ccaaatagaa ttccggcaac aaaaacgatc aatgcattga
      481 agtagatcga aatagaacca atcgactttc ctgttttttt acgaatcgtg atactcacga
      541 tatctagacc gcccgatgat aggccgtttc tcaacgctag cccaatacct agtcccatca
      601 cagccccccc aaacaacgca cagatgattg gatcggtaga taggactgtt tccggtaaaa
      661 tatggataca aagggaggtc aaagtgactg tgataaaagt gaaaattgta aacttcttcc
      721 cgatttttaa ccacgccaat acaaacaaag gtatattcag taaatacaaa gttgttgata
      781 ccggtacttc gaatcctatc agtgattttg acaaagtcgt caaaatctga gctagaccag
      841 taataccact tgaataaata tgtcctggct gataaaagaa attcaatgcg actgatgcga
      901 tcaacgcata aacgatagat accgaaagct tggtcgtgta atcatgataa gcccaatttt
      961 cgaatgtttt tttcatggaa aagccccgct ttatatgtta tggcttcatt ataaagtgtt
     1021 tttcaattaa atcaaggttg tcaaaagaag cgtattcgac aaatcctttt taccttttta
     1081 tcggctattc ttctatattc gttacatcaa tattcaattc gaacaattgt aaaggcgaaa
     1141 ccatacttgg tgcatcactc atcacatctg ctgcttttcc gtttttagga aaggcaatga
     1201 cttcacgaat attatcttct ccggctaata acattgcaaa acggtccaat cctaatgcaa
     1261 taccgccatg tggagggaat ccgtaatcta aagctgttaa caagaaacca aattgttctt
     1321 ccatttgttc ttttgtaaag cctagagttt ctagtacttt ttcttgaagc tcacgagtat
     1381 gaatacggat agaacctccg cctaattcat aaccgttcaa gacgatatca taagcttctg
     1441 catagacttt tgcagggtct tcagccaata acgggatatc ttcttctttt ggcatcgtga
     1501 atggatggtg agcagagaca tatcgacctt cttcttctga aaattcaaat tgtggccaat
     1561 cagttaccca taagaaattg aatttcgatt catcaattag tcctaattct tttcctaaac
     1621 gtgtccggat agcacctaat gcagcagcga cgatttcact tttatctgcg ccaaacatca
     1681 gcaagtcacc tggttttgca tctgttgctt taatgattgc ttctgttgct tctcccatga
     1741 atttagcaat tggacctttc aatccgtctt cttctacttt cagccatgca agccccttag
     1801 ctccgaattg tcctacatat tgacctaact gatccatgtc ttttcgtgag tagcgatctg
     1861 cagcgccttt agcattcaat gctttcacga caccgccgtt ttcaagagcc atttggaaga
     1921 ctttgaattc tacttctttg actgtatcgc ttagatcgat caattccata tcaaaacgtg
     1981 tatccggttt gtcgctgccg tagcgagcca ttgcttcatc ataggtcata cgaggaaatg
     2041 gcaatgtgac ttcgataccg cgaacttctt tcattacctt cgcaatcaat ccctctgtgt
     2101 aagtttggat ttcttctgct gttaagaacg ttgtttcaat atctacttgc gtaaattcag
     2161 gctgacggtc tccacgtaaa tcttcatcgc ggaaacaacg aacgatttga taataacggt
     2221 cgaatccagc attcatcagc aattgtttaa acaattgagg agattgcggc aacgcataga
     2281 aatgtccagc atggacacgt gaaggaacta ggtagtctct tgcgccttct ggtgtagatt
     2341 tccctaggta aggtgtttcg atatcgagaa agtcattatc atctaaataa tgacgaatcg
     2401 cttttgttgt cgcatttctt aatttgatgt ttttagtcat ttttggacga cgcagatcca
     2461 aataacgata tttcaaacgt atttcgtcac tgattgcttg atcatcttca atttgaaatg
     2521 gcggggtttt tgcttgattc aaaatcacga tttcacttgc aatcacttcg aattcaccag
     2581 tggccatttt aggatttact gcattttctc cgcgtcttct aacttcccca gtcacttcga
     2641 tcacgtattc actgcgacat ttatccgcga tttcccatgc ttctttgttg atttcaggat
     2701 taaagacgat ttgaacgatt ccttcacgat cacgtaggtc gataaagatt actcccccaa
     2761 ggtctcgtct tttttgtacc catcctttta atgtaactgt ttgttctaat tgttgttcgg
     2821 aaactaatcc gcaatagatc gttctttgtg ccatttattt ttttcctcct actcgaataa
     2881 agtcatttca tcatagactt catcaaaatg ttcatatatc tcttctaatg gaaaactttt
     2941 ttcttttcgg cttgccattg ctttgacatt aatcgtttga ctagcaagct catcttcacc
     3001 gatcaccaat actaatttgg cattcgcttt atctgctgtc ttaaattgcg cttttgcttt
     3061 acggttcatg aagtcgcggt cagctgaaaa accaaagttt cgaatcgctt ggaccacttt
     3121 caatgcttcg atatttgttt cgtctccgag tgcgaccacg taagcatcca actcattaaa
     3181 tgcagggata actacttcct ctgcctccat agtcaagagt aatcgctcga tacccattgc
     3241 aaatccgaag ccaggtgttg aaggaccacc tagttcttcc actagattgt cataacgtcc
     3301 gcctgcgcaa atcgttgctt gtgcccccat ttttggtgcg tcactcatga tttcaaaaat
     3361 cgtatgtgtg taataatcta gtccgcgaac catattactg tcgatttcat aaggaacagc
     3421 caatgcatcc agcatggaaa caacggtatc aaaatgtttt tgagcaggct cgcttagata
     3481 atctaagata gatggagctt ctgccacaaa tttttgatct cgtttgtctt tactgtccaa
     3541 tacgcgtaat ggattttcat gaaggcggcg tttagagtct tcactcagct ctgattcaaa
     3601 aggtaaaaga tagtcgatca atgcttgacg ataggcttga cgcgtttctt tatcacctaa
     3661 tgaattgatg actagccgga tttgcgtgat ccccaactgt ttgaagaaat caagtgccat
     3721 caccatgctc tccacatcag ttgcagggtt ttctgatcca aatgcttcta ctccgatttg
     3781 atggaattga cgtaaccgtc ctttttgagg gcgttcgtag cgaaacattg gtcccatata
     3841 aaaagttttg aacggtttcg catactctgg gccgaacaat ttatgctcaa caaaagaacg
     3901 aacgattggt gctgtacctt ctggtcgaag cgtcacatgg cgttcccctt tgtcataaaa
     3961 atcatacatc tcttttgaaa cgatatccgt tgtatcccct acacttcggg atatcacttc
     4021 atagtgttca aaaatcggtg tacgcatttc attatactga taatctctga ataataaacg
     4081 tgctgtttct tcgacaaact gccatttttc tgattcgcct ggcaaaatat catttgttcc
     4141 ttttggtctt tggaaactca tagttttctc tccctttctt agctatgtaa ataaaaaaat
     4201 cacccttgtt catcatgaac aagggtgaat gaaattcata cacggtacca cctaattttg
     4261 aaagatttct ctttcctctt acgaataacg ctcgtacgcg ggacggcttt tcactcgtcc
     4321 atctccagag tgtctttagg ctaatgaaac tttagatcat tttcagcctc gatgatcctc
     4381 tcttgtaaag tttcaaaaac ttactcgctc tttcattgac attcgatatt acttataacc
     4441 tactaaaaac cagtctcaaa gtcaagagat atctgactat ttcccctcac aaaaacgttt
     4501 tagttcagct ttttggtatt ttgttttaga ctagacaaaa aggaggaatc agctatggca
     4561 gaaataagaa tcggacattc acaagtctac gcagaacagc ttggattagg agcaaatgcc
     4621 gttggaggac ataatctttt tgacggtttg gaagatgaga caggaaaaca agtagttcgt
     4681 acagctttga atagcgggat caatttgatc gataccgctt atgcttacgg aaacggccgt
     4741 tccgaagaac tgattggcga ggtactgaag gaaaaagaat atgatcgctc acgtgtcgtc
     4801 atcgctacaa aagctgctca tgtaccaaat aaagggcgaa catttgataa ttctcctgag
     4861 tttctcaaac aatcggtgga agatgcacta aaacgtctgc aaactgatta catcgacatt
     4921 ttttatatcc actttccgga tgaaagcaca ccaaaaaatg aatcagttgc tacattgcac
     4981 gaattaaaag aagcaggaaa aatccgagca gtcggtgtgt caaacttcac gttagaacaa
     5041 ttgaaagaag cgaatgcaga tggatatgtc gatgtagtag aagataaata cagtttgatc
     5101 catcggcagg ctgaaaaaga attattccct tatcttgaaa aaaacaaaat cagttttgtt
     5161 ccctattttc ctcttgcttc cggtttgctg acaggaaaat atgaacttgg tgaggaaaaa
     5221 caatttggtg aaggagatcc tcgaaaaaga aatccagatt tccaaggaga acgtttcaga
     5281 gaaattttaa ccgctgtcga tgtacttcgc cccattgcta aaagatacca agccactcct
     5341 gcgcaacttg ttttagcttg gtacatgaag aatccacgag tgtctgtagt tatacctgga
     5401 gctaagcgtc cagagcaagt atctgacaat gtccaagctt tagatttgca tttatccaat
     5461 gaagattatc aaaccattga tgaagcattt cgtggatttt gatcaattga ccttatcaat
     5521 ttatagtaat atgaaaaaag gaagatcagc actgtttgat cttccttttt ttcgataagt
     5581 ttatgctaat ctaattcttg taaagtagcg ctcggctatt cggataagtc ggaaactgat
     5641 tgaatggaat cgtccaatct tgttcaggta cagagaactc aaacttattt actagctgat
     5701 cggaaaacac acgtagacta tgaagagtga tccattctcc tgcacagcga tgcatttgtc
     5761 gaaaatttcc cccgccttgt gcaatcatct catattcttc tttataggaa atatcttttg
     5821 ctttccctac ataccgtttg atcataaagg aatctggcgc ttcaactgtt cgttcatcat
     5881 ggtctgttcc atataaatct aaaataaccc aactgccttc tggtatacga taaccatcta
     5941 cttctacttc tttcagcgaa atcgcaggta gcattgggaa gaaaggataa tagcgtctca
     6001 tttcttgaat aaacggatct tgtaacgtcg aaaagtcatt ttttaattgc tgatacaaat
     6061 ctggtctact gaaaagtgca tgtcccatca atgccgccca cacagttaga gcaaccgttg
     6121 gacggatgat gttcaataat tcaacagctg ccacttccag aggtaaaagc tgaccatcta
     6181 aatctgtggc attggcaaaa gcatataatg ccacattttc ttttccagcc acaggatttt
     6241 gtcgagcttc ttcaatcaag ccttgcgccc atttttctga ttttttgcga ttctcgacac
     6301 cttttatgtg atcaatcgga gaagtaaacg ttccgctgat catagagatt tgatattctg
     6361 ctaatttttc gacctcatct ttagaaatcg ctgataaatt gatacctgcc cactcgcaaa
     6421 tagatgtaaa gaaaaccatc ttacttaaat caaatagttc aaattgacca tgctgtgctt
     6481 caagtgcttg tgtcagattc ttatctaaga tacgatgata atcttccatt ctttccggtg
     6541 tcattagatc cataaaaata tttttccgat ggtgatgagc agctccatcc aaagtctgta
     6601 ctccaccttg accaaaaagt gtcttcaata ctaattttgg catagcacct tttcgtttga
     6661 aattccttgg atcataaaat acttttgctg ctgatgatcc gtagatcgtg atagcttctt
     6721 tattgaaaat ctttgctttg acgacgggag cgtccgcttc atggcgtaac tcctccaaca
     6781 tgttgtatcc tttttgatac aattttttta aatctgtgat tttgatatct actactggta
     6841 cttctttcat cttcagcaac ctcctataaa aaataacttg tataatttaa gcttaacttt
     6901 ttttccgact gctgacaaaa gaagcgcttt ttgcagacaa aaaagccact taagttagag
     6961 tttatcagtc taacttaagc gacgtgtttc atagttttta ctagtgaaaa ccagattact
     7021 ttatttgttt ttagaatcaa taatgatcgt tacaggtcca tcattgacga gagaaaccga
     7081 catatctgct ccgaattgtc cggtagcgac tgtgatgccg cgacttctga ttccttcatt
     7141 gaatgattcg tataaaggaa tagctgtttc tggtcgtgcg gcttccacaa agcttggtcg
     7201 atttcctttt ttcgtatccg caaacagtgt gaattgagag atacttaaaa tttgtcctcc
     7261 cacatcttct acactaatat tcatttttcc ttgttcatct tcaaaaatac gcatttgtgc
     7321 aatttttttt accagatagt ccacatcttt ttgcgtatcg gtttcatgaa tgcctaataa
     7381 aacaagtagg ccgtagccaa tttttccaat ttcttgctga tcgactgaca cagcagcttt
     7441 acttacccgt tgaacaactg ctctcatatc tgtcctccta tccattcgtc cggcgtacac
     7501 tgtagacatc aggtactgct ttgattttat ccacgatact ctttaggtgg gataaattct
     7561 ggataccaac cgtcaaatgg atcgtcgcca ttttatcttt attcgtccgt gcttcaacgc
     7621 tgttgagatt tttcgttagc gcattgactg tttgaagtac atcattcaat aagcctgaac
     7681 gattatagcc gtaaatttcc aaatctgcat catactcttt tcgtgtattc gatgtatctt
     7741 cccattcgac ttcaatcaaa cgttctgcta catttggttt gtcaggctga acattcggac
     7801 aatctcgacg atgaatggat atccctctgc ctttagtgat ataaccaacg atatcatcac
     7861 caggaatcgg attacagcag cgactgatac gaattagtaa gttttctacc ccttgaatga
     7921 caacgccacc ttcatgacgt actttcattt tgtcaggttc ttttttcggc tgattcatga
     7981 tttcttctgc ttcttgcttt tgctgctcga ttttttgttc tttacgttct ttttcagtca
     8041 gacgattggc catcgtcaag gggctaactt ctccataccc tacagctgca tagagatcat
     8101 cttctgtttg ataattaaaa cgatcgagtg cttcttgcag tttgttcttc gtcaaaatat
     8161 ctttaggcgt aaatcctaga tctgtaatac atttgaccac ggattcgtgg cctttgatca
     8221 cattttcttc tcgatcttgg gctttgaaga aacgtttgat cttatttctt gctttgctag
     8281 tagcaactaa tttcaaccag tcgcgacttg ggccaaatga atttggagaa gtcatgatct
     8341 caatgatatc tccgtttttc aatttgtaat caagctgcac cattttgcca tttacttttg
     8401 caccagtggt tttgttaccg atatctgtat ggatactgta tgcaaagtcc aatggtccgg
     8461 atccttttgg taactcagta acgtctcctt ttggtgtgaa gacgtagact ttatcactaa
     8521 agatatctcc ttttacgcct tccataaatt cagaagcatc atagctttcg tcttgaagtt
     8581 cgagtatctc atggaaccag cttaattgtt tcgtcatacc atctggttct actttttcat
     8641 tttttccttc tttataggcc cagtgtgcag caaccccgaa ttcagcgatt tcatgcattt
     8701 cctgtgttcg gatttgaatt tctaccggat ttcctgccgg accgatcacg gtagtatgca
     8761 aagattggta catattagct tttggcatgg caatataatc cttaaatctg ccgggcattg
     8821 gtttccattt tgtgtggatt gctcctaata cagcataaca atccttgatt gaatctacga
     8881 tcactcgaat agctagcaag tcataaattt cgttgaactg ctttttctga tctttcattt
     8941 tacgatagat tgaataaatg tgttttggcc gtccatagat ttctgcaaaa atccccagct
     9001 cttctgtcgc tattcgaata tcttcaacag taccacttac gtatttttca cgttcttctc
     9061 tcttcgtctg catcaagtgg acgatacggt aatactgttt tggatttaga taacgaagtg
     9121 ctgtatcttc taattcccat ttgatccggc tgatccctag acgatgtgca agaggcgcat
     9181 agatttctaa agtttcctga gcgatccttc tttgcttatc ttctcgcagg tgcttcaacg
     9241 tacgcatgtt atgtagtcta tcagctaatt tgaccatgat aactcgtaaa tcttgagcca
     9301 ttgctagcag catctttcgg tggttttctg ctagctgctc ttcgtgggat ttatatttta
     9361 ttttccctaa tttggttacg ccgtccacta acatagcaac gtcatcgcca aattcttctc
     9421 tcagatcttc taaagtgatt tctgtatctt caacaacatc atgtaagaag cctgtagcca
     9481 ctgtatgggg atccattttt aaatctgcta atatacctgc gacttggata ggatgaataa
     9541 tgtagggttc gcctgatttt ctaaactgtc cgtcatgtgc agcagtcgcg tattcgcatg
     9601 ctttctcaac aaatgctaca tgttcgggac ccatgtattg actaacgagc tttatgacac
     9661 cgggtcccgt taaaatttct tcttttgtca caggaatcga tccccctttc ttttttcggt
     9721 aaaacgggaa tcgggacaaa tatctgtttg ataattgtcc caaaatccgt gcagttatac
     9781 gtttttttac ggaaatgttc gctttttaaa agctttttca aacgattttt cgtatccatt
     9841 gtataaataa ttgtttctat tatacaaatt gttgactatt ttttcaattc tttctgtcga
     9901 tactctttta ttaatgaaga acgttagctg aaagaaggat tcattaattc ccattgatag
     9961 ctagcagcac ttaataaata aagaggtgct gtttctgtac gaaggattct tggtcctaat
    10021 ccacaaagta tccctttgtg ttctacaaaa taatcgactt catcgcggga taatccacct
    10081 tctggtccaa agacagctaa gattcgacta tctggttttg tttcactaag aattttggct
    10141 gcatttgatt tttcgccttg ctttgctgat tcctcataag caaccaagac cacgtcataa
    10201 tcatctagcc gctcaaataa atcttgtttc gtttccaata actctactga tggcacgatc
    10261 tgccgatggg attgttccgc tgcctcttga gcaatctttt caagccgttg ttgtcgtttc
    10321 atacgttttt ttgcatccca tttaacgaca gaggctgatg ccgggaaacc aagaaaagct
    10381 tgcgctccta attcagtgga tttctgaacg atccaatcca gcttttcccc tttaggatag
    10441 ccactcgcta tggtaatcga acttggcagt tccttttgat tttcttcttt tttaatttct
    10501 ttcagataaa cagcctcttc agtaatctcc gtgatttctg caacaatggc tatttgatca
    10561 ttgaatgcca aaaaaacttc atcctcgggt tccatcctca taacccgtac aatgtggtga
    10621 tagttttccc cttctaaaac aaaatagtct tttgattcat atgattcttt taaaaaatac
    10681 cgctgcatct attccacctc tggttttttc aaaataattg cataccagtc tttttgctgg
    10741 aaaatttgat ccacgataaa cccttgttct aacatggcat ccagaaccat ttgcttcttc
    10801 tcttcaatga ttcctgaaac gatcagcgtg cctgtttgct tcagtaaacg ccaagcatct
    10861 gggatcatca ataagatgat atctgctaaa atgttcgcaa cgattacatc actttcaatc
    10921 tcgattcctg tcaaaagatc gttagctgat acatggacat ctttggctat ttcattcata
    10981 tccatatttt ctttcgccga acgaacggct acttcgtcta aatcaaaggc atagacatct
    11041 tttgcaccaa gatattttgc tgcaatactt agcacgcccg acccagttcc cacatctagc
    11101 aatgtttcac caccacgcaa gactgtttct agtgctgtca atgtcaagga agtcgtggga
    11161 tgagtgcctg tgccaaatgc cataccagga tctagtgtga taattttttc tagaggatct
    11221 tctgtctggt agttttccca gcttggcgca accgtcagca ttcgcgtaat acgcaccgga
    11281 tgatagtatt tcttccaggc tgttgcccag ttgctttcct ctacttcact aactgttact
    11341 ttattttttc caatagctag accaaactca ggtaattgtg tgatacgatc tttgataaaa
    11401 ggcaagattt ccggtaagaa agtcgtttcc ggaaaataag ccatgactag tgcgccttct
    11461 ttcagcgatg taaaatcatt tttatctaaa atctcaccat attgatccga ctgaaaatgt
    11521 tcaacatcta aagaatcttc aatcgcgaca ccactcgccc ctgcctccat caaaatatta
    11581 gcaactgctt cgactgcttc actttcagtt tctactttta cttcattcca tttcatatct
    11641 gtcactcctt aataacttgg gtacggtaaa taagtatctg cttcattttg ctgttttact
    11701 tctactaatt ctgctagttg ttgattatcc cagtgaagct cgaatacagc ttcgtcatcg
    11761 tcttcatcca aaaaatcaag caaatcgctt tgcccttgag ctaatacttc atttagatat
    11821 tccactaaag aatcagctac tgattttgcc aacccttttt ttccttcata agggattact
    11881 gctagataat tgcgttcatc aaaaacagat ttctgaggat taaaaagaag tacgccgtct
    11941 tcaaattcga taattggttc ttgcgaaacg atcccttctg catcatcaat ctcaaattgt
    12001 gctttatttt ctgcaaacaa gcgaacaacg atctcgatcg tatgattttt tgtatcccaa
    12061 tctaaagcta catcatagtc tgtcacttgt ttttcaatta tttcatctaa atatgttaat
    12121 agatccttct ttttcatttt ctttcctcca accccaatct ttctcgattt ggttttttct
    12181 ttgtaaagag atataaggat ttcccgacac aacaaaacgc aacggctttt ctgtccatac
    12241 acctttgttt gggataccga ttctaggcaa agcattgatc ttttttggtg tctttttttt
    12301 ctcaaaaact aagcgtaacg aactatctgc tatagactga ccataaagtt cttgaggaat
    12361 tgctaatgct tccactaatt ttcccggacc gttgctgata tctggaccag tttttccgcc
    12421 acgattttta ctcatttgat caatcattgc ggcgggttcg attgcacgta tcattactcc
    12481 ttgaggaatc ccttctggct gagtaatgat gttcaaaatc cgatgagtat gcatcgtgta
    12541 aaggtagatc gtacctggtt tttcatacat tgcccgcaca cgcggtgttc ttcttaaacc
    12601 atagctatgg gcggcctcgt cttctggacc taggtatgct tccgcgtcta caatatatcc
    12661 agctaatctt ccttctggtg tgtcatgctc caaatacatc cccaataaat cccgtgccac
    12721 ttctggcgtc gttttattcc aaaaaaattc atctatcttt tgtttatccg tcatttttct
    12781 cacctacttt atcatacaca atattagttg gataagctat gaaaacaaaa caacagaagg
    12841 ctgttctttc taggcatctt gctaaatatg atataattaa gggacgtaaa aacaagaagg
    12901 aggaaaatcc tatgcaacag ccattggctt atcgcatgag acctcgaacg atcgatgaag
    12961 tcgttggtca gcagcatcta gtcggggaag gaaaaatcat acgccgcatg gtcgatgctc
    13021 gcatgctctc ttccatgatt ctatatggac ctcctggtac tggaaaaacg agtatcgcaa
    13081 gtgcgatcgc cggctctact aattatgcat ttcgcatgct aaacgccgct acagattcaa
    13141 aaaaggatct acaagttgta gcggaagaag caaaaatgag tggtacagtg atcctcttat
    13201 tggatgaagt acaccgatta gataaaacaa aacaggattt tctgcttcct catttagaaa
    13261 gtggccgaat catcttgatc ggagcgacta ctgagaatcc ttatattacc atcaatccag
    13321 cgatccgcag ccgtacacag atctttgaag tcaaaccatt aacagaacaa gacatccagt
    13381 tggcagtcga acatgcttta agggataaag aacgcggcct tggacaacaa gcaatccagt
    13441 tagacgaaga tgctcttctt cacctttcgc gtgcaacgaa cggcgatttg cggagtgcat
    13501 tgaatgggct ggaactggct acactatcga ctccttcaga taaagaagga aggattcatc
    13561 ttacactgtc gatcattgaa gaatgtgtcc aaagaaaagc tcttacccat gacaaaaatg
    13621 gagatgctca ttatgacgtt atttcggctt ttcaaaaatc gattcgtgga agtgatgtgg
    13681 atgctgcact tcattatctc gcccgtttag ttgaggcagg tgatctggca agtatttgcc
    13741 ggcgtctgat ggttatcggt tatgaagata taggtttagg taatcctgct gctgcggctc
    13801 gtacagtgaa cgctgtatta gctgctgaaa aattgggatt gcctgaggca cgtatcccac
    13861 tagcagacgt cgtcgtcgat ctttgcttgt cgccaaaatc caattctgcc tacatggcat
    13921 tagatgcagc acttgctgat attcgagagg gcaaagctgg agatgttcct gatcatttga
    13981 gagatagcca ttacaaaggt gcgaaatcac tcaaccgtgg cgtgggttat cagtatcctc
    14041 atcattttga tcaggcttgg gtcaatcagc aatatttacc agataaactg aagaatgctc
    14101 agtattatca gccaaaagat acagggaaat atgaacaggc tctgggtcaa cagtattacc
    14161 gtataaaaga gtggaaaaag catcctccaa aaaactagac aagaaaacgg ttctgtttct
    14221 gcgagctctc tcattgctct ttttaaattc atatgctact atagaggtgg gaaatctgtg
    14281 atgttcgaat tgtcctcttt gtgtgttgac cgaacatttt tattgatatc ttgggacccg
    14341 tcttgtgtca ctgctggtaa catgccttat catttggtag ttcgaagcta cgaccatggg
    14401 acccacctgc ttgaattcgc gggatcaata ctacaggaca aacaacggca ttaacggaga
    14461 ttaccatctt ttaaagaata aagcaatttg ctcttggcaa attgcttttt ctatttgttg
    14521 cttattttgg aggatttgtt catgattagg attttaatga ttatcgccac tttagtatta
    14581 ttattcgtta gctattattt atttaataaa caggatatct tttttgtatt gatcaacaaa
    14641 aatgataaaa atcaaggatt cctacaattt tacggtgctg catatgctgt tttaggcgta
    14701 atgggtattt taacagcttt tttcaatcaa cgctttattg ccttggtctt tttactgatc
    14761 gtaattattg tttctgccac cttcagtatc cgatttgcaa aaaaaattgc tgaaccaaag
    14821 cagtaaataa ttctcttttt ccctaaagtg ttctacaata aaagagtaca atacgattga
    14881 cttgaggagt gaatgcttat gttacaacaa tacaaaaaaa tcatggttgc cgtagatggt
    14941 tctgatgaag ctgaattagc ttttaaaaag gcggtcaatg tagctatccg aaataacgga
    15001 gaacttctat tagcacatgt cattgacaca cgttctttcc aaaccgtttc atcctttgat
    15061 ggaatgcttg cagaacaagc tactgaaatg gcaaagcaaa cattggcaga ttatgaaagc
    15121 aatgcgaaga aagctggctt gaataatgta acctctgtcg tagaatatgg ctcaccaaaa
    15181 caaattattg ccagagaaat cccagaagat aatcaagtag atttgattat gttaggagcc
    15241 acaggattga atgctgtaga acgtttattt attggttctg tttctgaata tgttattaga
    15301 aacgcagctt gcgatgtgct tgttgtccga actgatttag aaaaccaatt gccagctaaa
    15361 gaccacacca aagcttaaca cgtagacgac ctgccaacta tcggcaggtt tttttgcaca
    15421 aaaaaccgtg ataaaaacaa aagacgcggc aagcactcat cggtctttat aatgtttaaa
    15481 ataaagggaa aacagtcaat gagttcaagt aaaataaaaa aagtgaacac cagggcgatt
    15541 agtcgccctg gtgtggttta aaacgaccta agcatgccaa agcttattgg ttcgtcaatg
    15601 tggaagtttc cgatgaatct gatacgaaaa aatatgaagt atcgatggcg gataccgatt
    15661 ttatcgccaa actaaaattt tatttcgggt tcgggtaccg cgagaaggca agtgctacat
    15721 tcggtaaatc attcaagaat tgatgaaagg ctaccgcttg ccattgcaac cacaacgata
    15781 ctcctgaacg cgatgttggt gagatttcca aagagaagag ctgtttaatg caacggtatt
    15841 aagcaaatgg caaaaggaag tcatgtagac aaagtttttt atcaaacacc atatgatttc
    15901 gcctgaacgc tggtttagct cagaatacaa tgatgtggtc cacgagacat taccctcagt
    15961 cagataaaaa aagctctctg acagaattac acatcgataa atattaagta aatgtcagcg
    16021 ctagttgaac tttctgacac tttcaggtac actggaaaag acaactacac gaaggaggga
    16081 aaatactatg atgcgcatga ctaaaataac aagtaatgaa aattaccaac gataccatat
    16141 cgcatttagc catgcaattg gttttacagt tgctgttgac gggacaagtt tgtccaaaac
    16201 agttgaattc acgtaaaatc aatggccatc tcaaggtatt tttctcatga taatctgagt
    16261 ttaaataaca caggccaggg ataaccatgg cctttttgag tggtctcaag gagagagatc
    16321 atgatcatac aagcaatttc tattaaaaaa acaatcggcg ggaatccatt atttgaggat
    16381 ttatcctgtc aaattgaagc tggtgaaaaa attgccttag tcggtatcaa cggaactggc
    16441 aaatcaacct ttttacaaat cttgaccggc agagaaggtg tcgacagcgg tgttatcagt
    16501 cgcaaaaaag gcttaaagat cgggacggtc gaacaagaac ttacagtttc cgaagcgacc
    16561 gtcaaccact acttgctcca ttctgccgct gagatacagg acctcaaaca gcaaatgagt
    16621 cgctacgaag cattgatgac agaaccagat acaaacttag agaagtgtct ggcccgttac
    16681 ggtgagctgc agcatcgctt tgaagaacta ggcggttacg tattagaaga ccggattgca
    16741 gcgatcttgc aaggcttagg gattccacac cgtcaggaag cacggttaac agaacttagc
    16801 ggaggcgaac gagttaaagt cgctttagcc aaaattttag cagccgatgc cgatttactt
    16861 ttactcgatg aaccgaccaa tcatttagat cttaacagta ttcgctggtt ggaaaactat
    16921 ttacagaata caaaaaaatc ggttctggtc gtttctcatg atcggcaatt tctcgatcaa
    16981 gtcaccacaa aaacatggga actggaagaa ggtgcattga ttgagtatcc cggaaactac
    17041 agccgttttc gagtcttgaa agaagctcgc ttggctgagc tgacaaaaaa ttacgaactg
    17101 cagcaaaaag aagtccagcg gttgaaagtc atgattcgtc gttttcgtca gtgggcacat
    17161 gaaggagaca atgagtcctt cttcaaaaaa gccaaagaac tggaacgccg cctggcgaaa
    17221 ctgacactcg tcaaaccgcc acctcccccg aaaaatcggc tgcaaagctt gtctaatggt
    17281 ggaaaatctg gcaaagaagt tttcatcatt caaaacttgc accagcagta cgctgatcaa
    17341 gtactattca aagacagcag ttttgcagtc tatcgtggag atcatttagc catcatcggc
    17401 gacaatggtg caggaaaatc aaccttactc aaattattac tcggtgagga aaagccttac
    17461 ggtggcacca tcaaattagg tagtagcttg caaatcggtt atttgccgca gcagctgcag
    17521 tttgccgatc ccgatagccg tttgctggct tacgccatca cattgactgg aaacgaagaa
    17581 gtcgctcgac ggcaattagc caaaagtggc ttttatcaaa gcgatgtggg taaacggatc
    17641 aaagatttat ccggtggtga aaaaatccgt ttggcgttgc tgaaactctt tttagaaaaa
    17701 atcaacgtcc tgattttgga tgaaccaacc aatcacctcg acagctatgc tcgagaagaa
    17761 attgaagaaa tgctgcaaga ctatcaagga acgctcctag cagtcagcca tgaccgctac
    17821 tttttgcagc aacacttcca agaagcactc gttattgatc aggagcagat cagccgccag
    17881 ccgctcggcc aactgattga ctaggacgtt ttttcgcaac aaaaaacttg attttcatac
    17941 ctgtgctttc acgattcagg caaagtacag gtggaaaatc aagtttttat tattcgatca
    18001 atgaatgttt cggatcggtt ttctagctaa acataaagcg aagaataaag tcgacagccc
    18061 acgaggctga tctaaaaatg ttttttcttt ttacatatgt aatgtccctt ataaagatga
    18121 atgaaaataa aatattggga ttttcctgaa aatcaataaa taaaatgcca gtcttttccc
    18181 ttttgaccac ttttccaagc aaaattccct aagaagatcc tgtaaaaaag aagggaaatt
    18241 gaagttaaat taccattttg cggtcttctc cgcaaaaaaa acacatgcaa gaattccctc
    18301 ttgcatgcgt ttcatctttt atcctaacga atgctttggc ttagccgcgc agacgctcca
    18361 catcacgggc aatcattagt tcctcatctg ttgggatcac taatactctt acttttgatt
    18421 cttttgtaga gatatctact tcttcaccac gcaattcatt caacgctgga tcgatttcac
    18481 aaccaaacca agacatacct ttgatgattt cactacgtac atgtccgtca ttctcgccga
    18541 taccagcagt aaagacaatt gcatcaacac cattcatcgt tgtcacataa ccgccgatgt
    18601 attttcggat gcggtccacg aaaatgtcat aagctaaacg aatatgttct ttttcatagt
    18661 tcttttcaag atcacgcata tcactagaca atcctgttag gcctagcaga ccagatttct
    18721 tgttcaaaac attgatcatt tcatcaatat caatctctaa tttttccatt aaatatggga
    18781 gaacagctgg atcgatatca cctgaacgtg tacccattgt caccccagct aatggtgtga
    18841 atcccataga agtatccatg gatttacctc catcgactgc tgtgatcgat acaccatttc
    18901 ctaagtggca agtgatgatt tttagttctt cgatcggttt gttcaataat tttgccgcac
    18961 gttccgacac ataacgatga cttgtgccat ggaagccata ttttcttact ttgaaatctt
    19021 cataataaga caccggtaag ctatataaat aattttgctt tggcattgtt gagtggaaag
    19081 atgtatcgaa tacagccaca ctcaaaatgt caggtaaaat ctttttaaac gctttgatcc
    19141 ccattaagtt tgcaggatta tgcaaaggag caaattctgc taattcagca atgcggtcca
    19201 ttactttctc atcgatcact actgaatccc cataagtttc tcctccgtga acaacacggt
    19261 gccctactcc ggtgatttca tcataagagc ttaaaatttg cagttcaatc aactgatcta
    19321 acagcatttt tactgctact tcgtggtttt cgatatccat gatttgttcg aatttttgat
    19381 tatcgccgta cttgatggta aaaatagaat cttgtaaacc gatacgttcg acgatccctt
    19441 tcgcaactac ggtttcttct ggcatttgat ataattgcca tttcaaactt gaactcccag
    19501 cattgattgc aattgtttta gacatatgac tctccttctc gttgaatgat tacagagcag
    19561 atgatttcca gtttttaaac tcgttgaaaa attccgtcac cttctctggt tcttttaatg
    19621 aagcaagttt cacaagcaag acttctttta cttgttgtgc ttgtggtcct tttttctgta
    19681 agatcaaaat acttttttgc gattgtttat tacggaacaa ttcatctggc aattgaatca
    19741 ttccttgaag ataaccttct tctttaaacc attttttgat ttcttcactt tgatccgttt
    19801 ctaaaaatcc actcggcatc aaaaacaaac caaaaccatc cggcttcaca tatttcattg
    19861 attgctctag caataaatga tgtgcataac tatggccttc ttcggtactt gttaaaaact
    19921 cttttgcttt ttcgtcattc ggataataac cgattggcaa atcgctgatt gcaaaatcga
    19981 caggatctat taataattcc tgcaacccgt cttgatgaaa gtattgaaca tttgcttgtg
    20041 tcaaatcact tgtagaagct gcgacagcca acaatgtatc atcaatatcc acaccaattc
    20101 cttctgtttg gtaccccgca ttcgataggt ttaaaagaac tgttaataac aagttgccca
    20161 ttcctgccgc aatatccaga atcttaaccg gtgctttttt atttgtgtat agttgttcaa
    20221 taagaaaaac aaataaaaaa ccgatactat caggagtgag ttgatggttc gcctgcaaat
    20281 gttctgtctg gctgcccttc aataaaagta attgagacag tctgcgccat tcttctggct
    20341 cgaaagaaag tttcttcaac tcttcataaa gagctgtgat ccgttgcgtt gtctcttttg
    20401 tcggcactcc atccaccaca cgtacttgat aatcatctat taaattttct gcattttcga
    20461 cataggcttc taaaaaggaa gttcccaatg cattctgtaa aagctgaata gcttccaggt
    20521 tttgattaaa cccttgttcc attttttctg gtaacatggt gcacctcatt tctagatata
    20581 ttataacaat tttttcacct gtttaattct atcgaacaat tgacaaaatt tcaatcgttt
    20641 tcactgtttt ttatttgtga aacggcaaac atttttttat taaaaacagt gttataacag
    20701 aaaagagggc gttttcacga acattccatc attctgtact agtatctgta tcatttttta
    20761 cttcttctgt gaaccgaaag ataaacggtg agacttccac ctctaagaat agggactgtt
    20821 gtacccactg atatgttaca gatccagttg tgtagtaaaa aactccttcc gaactttcgg
    20881 caagcgtaga ctgttccgat aaagccatct gcttcattat ttttgctata taataatttt
    20941 tgattcgtat attaagatcc attgacaaac ggtagctttc taatgctgcc atcaataaaa
    21001 acgtacataa aagaaataaa agcaagacag aaaataaaat agagccctta taacattttc
    21061 tactgatcgt tttttgtcca tatggcatat ccatcttctc cattttcaaa gtgtacataa
    21121 aaagaaattg tgtgttcttt ttccaataat tgaagctcag tgatacttgt taacataggt
    21181 tgatgtccgc cggaagttct tcttttacgt atcattgcct gatatctttc gatgatatac
    21241 tgtttatcct cgatcgtgta gtgcagttgc tgatttttca cagagacatt gtgactgctc
    21301 ttgagttctt cttctaattg tgctaaaaat attagccact ctttttgatc tttacgctgc
    21361 aaaaattcac ttacttggat ggtttgctga atcaaaagat tgaatgtgag caatagactg
    21421 ctcaatatca tcagtccaat tagacattca atcagcgtaa atgctggaat tacttgtttt
    21481 ttcaatactg atcgcttctt tctccatatt gacaaacact ttttcgatta ctccattatt
    21541 atcgacaatt tggatatcgt atgggatcat ttcttccctt cgctctggtc ttttgtgata
    21601 catccgctgc gtcttgatat cttcataaat cattcgaata aatagcagtt cctccccact
    21661 tttcttttct gcctgaagca aaaacagctg gccaaagctc actaagtaga cacagacaga
    21721 agcgatcata agcgctgtaa gggactccaa caaaacaaat cctgttttat tcaattcttt
    21781 ttgtatagcg accactccct atctggaatt gatacgtaat cgtccgctct ttttcttccc
    21841 aataaaattg ataagctttt agtgaacttt cattcccggt tctagcagca aaagtaatag
    21901 aagcatggcg tttcaaggta atctcatcag gaatatctaa tacttcccaa tttattttat
    21961 ctggattcgg aacatgaaaa atgatttgat tctcttcact attccaataa aagcctgttt
    22021 ggatctgatt cacaatagcg attttttgcg tagaatagat tcgtttttca aattggccga
    22081 aaaattgata gactgccaat tcattcttcc atgcagaaaa tgcgagaact ggaaaagaaa
    22141 ggatcgttgt aagtaataaa atcagtaaga gtgattcaaa caacgtatat cctgatgcta
    22201 aaaccttacc ttcaaggccg ctgatagatt tctgcatgtt ctcgtttgat atagccttca
    22261 ttcaacaatt cctccacagt aggaactctt ccattatttt gaagcgcata gagctctttt
    22321 tgtgtgtcta ccaactggat gatcgcttca cgactttctt ggtctacatg attcctataa
    22381 cgcgacaaat tagggacgaa caacaacact aatacactga tgatcagtaa gacgattagc
    22441 atttcgatca atgtaaaacc gctataataa gatatttttc tctttttcat ggcaaaacct
    22501 cctctatgct tccgtaaaca ggtaataaaa cagcggcata aactaataaa atcaaagccg
    22561 ctacgcccaa aaaaatgatt ggctgaataa aatgcatcca gtgatgaaga cgtttgaaaa
    22621 gctgttttct tgtccattcg ctgtaaaaaa tcagttcttt acctaaactt ccctttgcct
    22681 ctccctgtaa aacaatttta ctgaattctt ctgatagaaa acgataattc tggaactgct
    22741 cagccaatga catacctgcc tccaatcctt gaatcaactt agaggcaaga tgctgaatca
    22801 gactttcttg tttcgtttcc tttaagcaga ctatgatttg ccttaattcg aaaccttcat
    22861 gaaaaagctt tccaatctct agtgacaaat aagccgattg atacaaggaa tatatcggtc
    22921 caatcaataa ttgtctgctt atccattcac tctgatcaac aggagacaac ttcatcagac
    22981 gataccttac tagtaaagcc aatagagtaa ccgctaggat gaatagtaga acataccaat
    23041 gaaagacttg tatcagctga ataccccaat gctcttgcat gaccattcct gaaccaataa
    23101 gctgaggcaa aacgaacaac cgcatactaa acaagatacc tagcaaaaag gtgagcaata
    23161 gcatcggata tgcaagcagc tttttcaaat cattgcgaaa tccttcaaca agccgaaact
    23221 gtttggcaat ttccaacaaa gttgtcggca gattcccatg acgttcagct aactcgatct
    23281 gtaccagttg ctctgctcca tatcctattt gcgaaagtac ctcactaaac gcttgccctt
    23341 cctttagctg atgttggata tttgccaaaa taacaggtgg aaacgaacgg atcgtgagta
    23401 ataaataaag ggattcttgc agactaaaac cagataaaag cagttctccc atggtttgtg
    23461 caaactggtt ttgctggctt cttgttaatc ctttagaata gttctgactg ccaaatattt
    23521 tcctgagctt cttcataact ttcctcccat gattttttta ctgattcttc ctccataaat
    23581 tgataactcc ataaaacaga aggatggtga ttggcatctt ccaaaagttc ttgataaatg
    23641 acaccagcta aacattcaga tagctcttct tttttctttg tcagttcatg gattctgacc
    23701 aatgtacctt caagattgcg tgcatgtacg gttgcaaaaa cccggtgccc tgttagtgct
    23761 gctcttaccg cagcatgtgc agttgattca tctcttatct cgccaatgat tagcaagtct
    23821 ggacgatgcc gtaatgcgag tttcaaaagt gtatcataag tctgtgctat tttcggattg
    23881 atctgtagct gcagaaaaaa cggttcttct atctctacag gatcttcgat agtaatcact
    23941 tgcaattttt cttccaaagc caaccgatac attagagaag tcttgcctga tccgactggt
    24001 ccgctgaaaa ggtataatcc acgtctgacg gttttctgta aaatagttgc aaaatctcga
    24061 gaactaaagc aacgatgggg tgtttcaaaa gtaccgtata atagccgaat cactaaactc
    24121 tctcgttgaa ggtaatctcc tacagaagac aaccgcagac gaacttcctg tttttctaaa
    24181 gaataagtaa atgcacctaa ctgagctttt cttttttctc cgatatccat ctgtcccaaa
    24241 tacttaaatc ttgagatcag ttgctgtcca tctgccgaag agactttctt ccagaatgtt
    24301 cgtttttttc catagcgaaa atagatgtaa aatacttctg aagctggcaa tatatatacg
    24361 tcttgggcat gtttggcaaa actttcactt atcatttgat ccgataatcg ttttatttcc
    24421 attattctcc cctccacaaa aaacatacgt ctttttgatc tttttgtttc aaataaaatg
    24481 aatttacaaa aaaatattgg caaaagccga atttatgtta tacttataaa aatttatctt
    24541 gggaggcact ccattggaaa caacattaag gaaatatatc gaagtagcaa gtaaaaaaaa
    24601 gcctgctgac ctagtgatca agcatgctaa gatcgtcaac gtctttacga aagaaatcat
    24661 ggaaaaagat gtagcaatct gcgaaggaat gatcgtagga atcggtgaat acgaaggaaa
    24721 aagcgtacat aatgctaatg ggcaatatct agtgccagga ttcatcgatg gccatgtgca
    24781 tatcgaaagc tcacttttat caccaagcga attttcaaaa gtatccttgc ttcatggagt
    24841 tactactgta gtgacagatc cccatgaaat aggaaacatt gctgggagta caggtcttga
    24901 gtttatgata gaagatgcaa gacaaacact catgaatatc ttttttatgc taccttcttg
    24961 tgtacctgta accccttttg agaccaatgg tgcaaattta aatgcaaaaa aattagcccc
    25021 ttttttacaa aaacccgaag ttttaggctt agctgaagtc atgaactatc aagcagtagc
    25081 aaataatgaa acagacatcc tagaaaagat ccaattgatg caccagcata agaaaaaaat
    25141 tgacgggcat gcagctggta tcggcatgga agagttaaat gtttatcctg cagcaggtat
    25201 tcggacggat catgaagcta caactgcaaa agaagctaaa gaacgtttgg atcttggtat
    25261 gtatttaatg gttcgagaag ggaccgttgc aaaagatctg gcatcgctac taccggcgat
    25321 cacacctgaa aattcaagaa gatgcttctt tgtcaccgat gataaactga tcaatgattt
    25381 agtaaaagaa ggatcaatcg atcatatcgt ccgtcaagct attgcacttg gaatcgatcc
    25441 attacaagcc attcagatgg caacattgaa tgcagcagaa tgttttgatt tgaaaacact
    25501 aggtgcgatt gcccctgggt atcaagctga tttcttctta acagacgatc ttaccaagct
    25561 gccaattcat gaagttttct ataagggaac attagttgtt caatctgggc aactccaaga
    25621 ccatttattt gctgatcatt caaattcata tacgtgttgt ctgccaaaac taaacgctca
    25681 accattaaat ccacattcgt tagagctgct tctttcttct tcacaagccc atgtgatcga
    25741 aattatcccg aatagtctag tgactaaaga tttggtagaa aaagtggacc gacaaggcga
    25801 acgattcatt ccttcaatcg aaaaagacca attaaagatt gcggtgatcg agcgtcacca
    25861 tcaaacggga aacattggat taggcatagt gaaaggattt cagctgaaac aaggagcgat
    25921 agcgacaacg attgcccatg actcgcataa tctagttgtc gttgggacaa acgacgagga
    25981 tatgctgtat gctgctaatc ttttgattca aaaaggtggc gggatgaccg ttgtcaacaa
    26041 acagcaggaa atagcttgtg tttctcttcc tataggcgga atcatgacac aggaaccttt
    26101 ttcgatcgtc tatgaacagt tgaatgaatt aataactaaa gcacatcaac taggagcagc
    26161 taaaaccttt gatccttttt tgacactttc tttcttaaca ttatctgtca tacccgaatt
    26221 gaagatcacc gacaaaggat tattttcatt ttctcgcttc caattgattc caccttgtgg
    26281 aaaaaccgat gaaataaata cattttaaat caaataaatc ccagaaaata cattacatct
    26341 gtaatgttca ttttctgaga tttatttatg gatattaatg tttttatgaa agcagaaagt
    26401 tagtttcaca gacttttctt tttggtctta tcttgaaatt gagcttctac tcgataaata
    26461 cgttgacctt ttgaagaaaa tttttcttca tactccgtca taatattgcc ttcaaattga
    26521 ctttgatgta aatctaacca tacctgtttg aggatcatgc catattgaga aaaacttgcc
    26581 agagaatact caaacaatcc ttgattatct gttttgaaat ggatttctcc atttggacga
    26641 agaatctgtt catctacagc aagaaaagtc ttatatgtca atcgccgttt ttcatgacgt
    26701 ttttttggcc atggatcgga aaaattcaga tagatttgat ctacttcgct gtcagcgaaa
    26761 tactctgtca acgctgatcc atccacatgt agaagtttca ggttaggaag cggttgttcg
    26821 atcaacttgt ctagtgcaat cgaaacaaca ctgacctgca tttctactcc aatataattg
    26881 atttcaggat gggcttttgc cattccagtg atgaactggc ctttccccat accaatctca
    26941 atgtggattg gatgatcgtt ttcaaacaat tcattccatt ttcccttcca caatgttgga
    27001 tcactaatta caaaattcgg gtgagccgct aacatttccg ctgctcccgg acgatttcgg
    27061 acacgcatta tttatctcca ttcctttcct ctaattaaaa aggtctggcc aaatctccgc
    27121 agagtttggg ccaaacctca gccactgaaa attcgtttta attgtaaaat ttcttcgttc
    27181 atccgtcgat gatcttcacg tttgcaatat ctggtgattt cttggagcaa attcatggcc
    27241 gcataccaat gaaatttttc aagcatttcg tcagtcgggc gtatgccata actgatcagc
    27301 cactgattcc agctagacag tggcacataa tttcctaaaa tcgttgcaat atccaccgca
    27361 gggtctgcaa acatgattga atcccaatcg actagatata aataattttt acatactaac
    27421 caattacgat ggtttacatc tccgtgtact actgataagt tttccttatc tattgacggg
    27481 atctgctcac gaagaaaacg atggacaatt tgcagaaatt gatttttctg gagttcttcc
    27541 ggtaattctt tttcatattt ttgcagcatc ttctccggcg tcatgacacg accaccgata
    27601 cgggtcagca tggttttaag agaatttgaa tgatgtaaat ggtacaaaac atcaatcacg
    27661 tcgtttcttt gaccaatctc attaggagtc agcaagcgac cttccagcca ctcttgtgct
    27721 gtcaaagtat cgccattccc cgttcgtttt gtccaaacaa gttttggtgt aattccttcc
    27781 cttgaaagag cagccaacat cggagtcgtg tttcgtttaa taaagacttt gtcctcatca
    27841 cgcatgccta tgtatgtttt tcctgtgtcg cctttgatgg gacgtaaccg ccactcttgg
    27901 tctaactgaa actccatcat gcattctccc gtcctcttac tctttgtttt gcagtactta
    27961 ttattctagt cgcctgaaca ctacacgtca agcacatata cgttcttttt catttaattg
    28021 ccattcaaaa aaccagcttt tctcaaaaaa aatagaacga acagcattct tactgtcaac
    28081 gctctatttt tcattctcat ttttacccta cacagctttg ttttcagtct gacatcttct
    28141 tcaaacgagt tggtacatat agtttaacaa agagcatagt aacaatcaaa taagccgcta
    28201 gcggaaccca tcgctgctgc ccatctaata cgactgcagc aatcaaacca aaaataaccg
    28261 acgcgataat taatacccat gtgagtagat actgaatcgc ctgcttcttc tgattcttat
    28321 ggattggata aagctgaacg agaaccatat agcggaactg gttcgctaaa ggtaacagtt
    28381 ggaaaccgat caaatataaa aataagaaac cgataatcag actaaaataa aagtcgacta
    28441 ctccggcaat caataaccca cctataatta ctaatcggaa ataaagacca ctaaaatcag
    28501 aacctcttgc caatcttctt gcaaacaagt aaagataagt gttttttgat tctcttttaa
    28561 tatgagtcaa tataggatcc aaatagcgtc ttcttttgat ctttgccgta atttcaggaa
    28621 catctgtgaa taaattgata aattgataga tctgatgcaa cctatgttct tctacatgca
    28681 ccattttctc ccaatccaat cctttttctt gttgcttcca caaaaaccag taatatagac
    28741 ttgcctgtac taatgcacct gctattcctg cccatattag ccaataaaat gatagaaaca
    28801 aaaagatcag agaagaaaaa aaccagccta atttccaata acgaataact tgttgatcta
    28861 gtcgaaacaa ttctgctctc ttgatttgca gatgactgaa tttcaaactc cataatatga
    28921 tcagatagac gaaaaacatc gaaaaggatt gctccgtact aaccaccact aatggcatgg
    28981 ccaatccgca tgccaatatc aaaaagatca acggaaaaat gcaagaatag cgaaaagctc
    29041 gttccaaata ggtttgcatt tctttttctt taggaagtaa aaaaacaata tctgctgcct
    29101 ctgcaaaagt agccatctta cccacgaata aacagagcat ccaaaataca gagattatga
    29161 gccctccaat tgcaaacggt ggctgcaggg ttttcaacca attagaataa tataatccca
    29221 caccgccaat caagaaaaga caaactaaaa caaaatgatc gttcaataca taacgcatat
    29281 atttcatcat tttcttttga tgtcttgcta aacgtttttg aaaaaaacta ctcatgtgcc
    29341 tgctcctttg ttaaagcaat atagatttcg tccaaagaag aatccggcag ctgaaattct
    29401 tttctcaaca tctcaagaga accgtctgct cgtatctctc cgttatgtaa gaccacaaaa
    29461 cgatcacaat atttttctgc agtagctaaa atatgcgtag acatcaaaat accggctcct
    29521 tcttttttca tttctaccat caattcaagc aatgcattga tagctagtgg atccaagcct
    29581 aaaaatggtt cgtcgatgat atataaagaa ggctcgatca aaaaggcgca taagatcatc
    29641 actttctgtt tcattccctt tgaaaaatta gctgggaacc agtcaagacg attagataac
    29701 cgaaaagtct ccaacaactt ttctgctctt ttcattgcta tatccatagg tatatcgtag
    29761 gccatcgcag tgacttcgat atgttctctc aatgtcaatt cttcatataa agaaggagtc
    29821 tctggaatat acccaatttg tttgcgatat tcttccgtat ttttctttaa ggatagatga
    29881 ttgatttcta tcgttccttt tgaagcttcc agcagaccga taatgtgctt gatggtcgta
    29941 cttttccctg ctccgttcag tccaatcaac cctactatct ctccaggctg tacatcgaaa
    30001 ctgatatctt ttaacacagg gagatgtccg tacccccctg tgacatgatc gataattaaa
    30061 ctcattcttt ttcctccgcc atttattttt atttattata ccacgaagaa aatgacggta
    30121 ttttgttttt ttccttacaa tagctttaac ttatggtaaa gttaagctga agtatttatt
    30181 ctttatagaa aggatgtttt atatggaaaa ctgtattttt tgcaaaatca tcaatcaaga
    30241 aatcccaagc tataaaatat atgaagacga caaggtctat gcttttttag acatctctca
    30301 agcaacgaaa ggacacacat tagttgtgcc taaacgtcat gtagcagata tttttgaata
    30361 tgatcccgaa cttgcaggag aggttttttc gcgtgtgcct aagattgctc aagcattgga
    30421 aaaagctttt ccagaaatga aaggtcttaa tatcttgaat aacaaccgtg aactagccta
    30481 tcaatctgtt tttcattcac atatccattt agtccctcgc tacagcaaag aggatgattt
    30541 ttctattcac tttgtcaatc atcaagatag ttatggatcg gaagaactaa aagctattca
    30601 agaaacaatc gtaaagcagg tgtcctgcga tgattaaaaa ctttttcaaa ggactgttat
    30661 ttggagctgc tgctggcact gtcggaggtc tgttattggc gcccaggagc gggaatgaaa
    30721 caagaaagaa actgatcgaa gaattggaag aagcaacaac tctgacaaat gatttaaata
    30781 acagcctgaa tcattttaaa agcgcattga tcgaaacaaa agaaacagct gaatctgtga
    30841 ttcctgcttt ccaagaagca atcaaaaaag atatcgaaga ttttaaattc caagccgaac
    30901 cacgtatcac acaaatcaat gaacaagtag aaaaactaaa agctgatctg ccagagtatc
    30961 ctgaacaaat ataataatga ttattttctc aacagattta actatataag tgagaaaagt
    31021 ttgcgtattt cctaggaatg tgatgaaatt ttgaattttg tttgaaacta ccacgacaac
    31081 gtttttaaag gaaatgggat atggtatagt taatatataa aaataataac taattacagg
    31141 agtgcaaatt ttaatgaaaa aaaaatcaat tattttggca gctactagtg cgttggctgt
    31201 tttaactcta gcagcttgtt caggcgatac taataaagat atcgcaacga tgaaaggcgg
    31261 aacgatcact gtatctgatt tctatgatga agcaaaactt gaatcatcga accaatcctt
    31321 agtacaacgg atgatcattt ataaagtatt caataacaaa tatggcgata aagtaaccga
    31381 taagcaagtg gatgcagaat acgacaaaca agcgaaatca ttaggtgata cttttgaatc
    31441 acaattagaa gctgctggat atacgaaaga tacttataaa gaatatattc gtaacaattt
    31501 agcttttgaa gctggcttga aagctcacgt agatattaca gatgacgatt tgaaaactgc
    31561 ttggaaatct ttccatccag aagtagaagc acaaatcatc aaattatcaa gtgaagatga
    31621 agcaaaagat gtgaaaaaat cagcggatga tggagatgat ttctctaaat tagccaaaga
    31681 caaatcaact gatacagaga caaaagaaga tggcggtaaa gttaaattcg actcaacgac
    31741 aacaactatt cctgcagaag taaaagaagc agctttcaaa ttgaaagacg gtgagatctc
    31801 cgatgttatt actacaacaa atcctacttc ttatgcgaca gaatactacg ttgtcaaaat
    31861 ggtgaaaaac caaaataaag gcaatgacat ggataaatac aaagatcaac taaaagatat
    31921 cgctactgaa actaaactaa gtgacaatgc attcacaact aaagtaatcg gtgaagaatt
    31981 aaaagatgca aatgtaaaaa tcaaagatga tgcctttgaa aatgtgttga gtgcattcac
    32041 aacaacttca tcaagtacga aagactcaag tgaaactaca gcttcaacaa agagttctga
    32101 cacaaaatca actgattcaa caaaagaatc ttcaactgaa gaaacaactg attcatcaaa
    32161 ataaaagaaa acaaaaaaag cgaagactta tgtctcgctt tttttgtttt gaatgaaaaa
    32221 aataaatttt tgctataaat cttttggtac atagaaactt cggttgtcca taccgaaaat
    32281 ccgatcggtg tattttcctg gttctgtttg acgaatcgaa gtcaacatca tctgcatcga
    32341 agcatcgata ttgtcgattt gatgtaatat ttctgcttcc attattcttg gacgtacagg
    32401 tgagccatat tctaataagc catggtgtgc taaaaccata tgacgtaata caagaacatc
    32461 ttcgtcagct tcgctgattt tcaacgcaag acaggcttta gttatttctt cgtcaatcaa
    32521 taccagatga ccgatcaaat ttcctgcaag cgtatattct gtcgacatag gaccggaaag
    32581 ttcaatcacc tttcctaaat catgcaaaat gataccagca tacaacaatg ctcgattcag
    32641 ctcaggatat tcatcggcga ttgatttccc taagtgaagc attgtcaaag tatgataagc
    32701 taatccactg gcaaatgcat gatggtttct ttttgctgcc ggaaactcaa aaaattgttt
    32761 ttgatattta ttaagtagat agcgaacaat acgattccaa tgcgcattgg tgatctcaaa
    32821 caacaattga ttgatttctt cttccatttc ttctttcttc aaaggggctc tttccatgta
    32881 taaactaggc tctcctggct catctaccct tgttaagcgc atatgaagaa tctttacttg
    32941 agggtttcct tgataaacct ctcttttacc gtttagaaaa acgactgtgc cggctgtaaa
    33001 tttattgatt tcctcctccg aagcatccca gtatttacca tcgatagtac ctgaagtatc
    33061 ttggaaagta aaagcaataa atttttttcc gtttttagcg attcttacat ctgcactttt
    33121 gatcaggaca aattgttcaa actgctcatc gattactaaa tcacgaattt ttttcattat
    33181 ttgctccttc taattgtttt acgatttggt tcatctcatg gtaatagctt accatttctt
    33241 tatcagaaga aaaacaaatt atttgatact tttctccaaa tttagctaaa agttcagcta
    33301 attgatactt tcgctgatga tcataatgaa gccagccatc atcaatgatg atcgggcata
    33361 tcgaacggtt agcttctaat gagaggtaag caaatctcat cgccataatc agctgatctt
    33421 tcgtacctgt agataactca tagatgggaa attctgtata tccgtctgtt aaagtcagtg
    33481 tatcattttt cagtaatatc ttctgataat ttccttttgt taaaatacct aaataatcac
    33541 ctgctgctct taacaactgg ggtaattgtc tttcactcat ctctgttgaa agatcaccta
    33601 agaatgtccc ggcgatttca agtgctcccc attcctccag taattcctgt aattttgctt
    33661 tttgccggct agcttgttga taaagttcgt ccaatgtgcc gtctctttgc aactcttcta
    33721 cttgaagttg gagtcgttgc cgattttcac ttgcttcttt ttcttcttgc tggagttggg
    33781 attgcttttc tttcagttgg cgcagtctta cagataattt ctcttttgtc agctcttcag
    33841 gaaatagtgg agtaagcatt tccgataatt cattgtatct tttcatctgc tgttcggctt
    33901 gtttggcttt ttgtaaaaag acaggtactt ccgaaggata agataagcct gcttgagtca
    33961 attgttcgct gtattcttgt atccatactt tttgtttctt tttcaattga ttgatttctt
    34021 gcttcaataa agtattttgc tgatagcttc tggcaaattt catctgttcc atctcttgta
    34081 taaaacggga aagcaatacc attttttctg aaattgtctt atcttgtatc ggtaaccatt
    34141 ctttaacaaa ctcaaatttt tggtcgattt gtcgtagttt ttcttgtatt tcctgttctc
    34201 ttttatgttt ccgctcaaat acgccatata atttctgata atctatgact tcctgtccat
    34261 attctttcat gagtttgagc gtatccatct ttcctaagtg ccgatgatcc gcaaaacgat
    34321 atacttcttg ctgaagaatc tgtttttctt ctccagttct cttttgtttt tcttctgctt
    34381 cagctaattg agctaaggct agatcaagtt gtcctagttt ttcttgccac attcctttta
    34441 tctcagagaa atcattttta gttttaattt ttggttgcct tttgtagaag aaatagccaa
    34501 tcatgcctgc accaattagt aaaaggatca ctttcaaggg cattggtaca aatatacctg
    34561 caatgattat tgctccgcca gccatcagca tatacggttt agatgaagag actggtgcta
    34621 tctctgactg attttgtaat aatgctggat aggtctcttc caagtcattc acttctgctt
    34681 caatcatctc ttgctgttct ttagctattt ggagacgcac agaaatctgg ctttctagat
    34741 cagcaatcct tctttcttca gccagaagat gcttccattc ttcttcatat aaaaattctt
    34801 ccggtaacgg atttttctgc caatgccacc tttgttccaa aatcttcaat tctgaatttg
    34861 tttctctagt ctcaatttgt aatctttgcc attcttcgtt taattgttcc gctgtaaatt
    34921 gaagttgctg gagatctttt agttcctgct cctgctctaa ataaaaataa tatctggctg
    34981 actgctgatc catcccgcta tgcttttcca gtgttgcttc taattcttcc aaacgctcat
    35041 ttgtttgctg ataccgtttg gagaaagact ccagatctaa aagaagttgc tcatcaggaa
    35101 gacctgtagt gctgtttttt ttgagagatt gccattcttc atataaagaa aaattaagtt
    35161 cttgctgtct ggctaatgaa aattgtttgt tcgcctcttt taattgtgcc aataactgct
    35221 gctgttgcga ctcgtattcc tgttcttgtt ggatcaactt gccaaaataa gcttcctgtt
    35281 cttgtttttg atgaattttt tcttgcagct ctttccattc atttaatttt tgattgatta
    35341 gtaatttttg gccttttttc ttgaataatt gttgtgcttt ctgataatat tcttgccgct
    35401 tttggaatac ttgagaactg cccgtgatgc ctaaagaaat cagcgcatca tgtagctctt
    35461 tttctctcaa atgatctagt tccatcaact gctcttgctg gaaagtaaat acctgctgga
    35521 ataactcttt cgtcaaaggt gcgatcagtt tttctagtaa ctcatcactt cccacttgat
    35581 cccccagcca caccttcgat tttccgcgat tttgctgctt gtagcgctcg acagcaaatt
    35641 caccatatac aggatgttta agccatattt tgccgccata ggctgagccg tcttttggtg
    35701 tataatcctt ttttttacgg ccttttgaag ggaaaccaaa taatattgct tgaataaatt
    35761 gataaatcgt tgattttcct gcttcgtttc ccccatagat cagctgattg cctgaataaa
    35821 agtcaaaact tttttgacgc cattttccaa aagcagcaat ctcgatcctt aaaatcttca
    35881 ttcctgatcc tcctcaaata aaaaatcttg attaagcagc tgcttggctt tttcaagtgt
    35941 ttcttcttga tattccggca gttcgctcaa tattctagcc gcttcttgat gactatatac
    36001 ttcatttaaa atttgttgga aatcttgagg tgtctgatag tattgaaata actgttccat
    36061 caatttagca gaagcagcaa gtggggtctt cgtataaaaa gtattctcta tgagagaaat
    36121 acgccaaata aagaaatcat ttatcctatc tgagaattcc tctaacaaat agtctaatag
    36181 ctctcctgat tgtatccgtt ccaaaacatc tgtattgaga tggtcataat cttttaattt
    36241 tatctcaatt aaactaaatc catcctctac ttttgaaagc tgctgacgaa tgtgaacaat
    36301 gacctctttt gtcgttcttg ctttcctcaa tgatatttcc tttgtcttcc aatagacctc
    36361 tgctacttta atcggatgta tttggcaact tcctttactt aattcaacta gtagaataga
    36421 tgtagaagct tgttcctttt tagtatgacc ttgaggcgca cctggataaa caattgtttg
    36481 cttttcattt aagaccgttg gcacatggat atgccctaaa gcccaataat cgtatccctt
    36541 ttcttgcatc tttgagggtt ggaaaggagc atagtttcct ttttgagaga cgccaggttc
    36601 cccgtggtac aacccaatat ggtaatctgt gagttctcta gaaggaaatt ccatcacttt
    36661 gtctttttga atccacggtt gacgataact aaatccactg agagagacag tttctccact
    36721 ttttatcgtt attttttttg tctctaccgt ttcggatgta aataaatgga catttttagg
    36781 aaaggagaac caataccgct ctttttgata gaaatcatga tttccgaata ttataaatac
    36841 aggtatatgg ttcttttcta accgtttcat ctgttctgaa aaatgtttct gtatttttaa
    36901 tgaaggacga ttttgatgaa aattatctcc tgcaagtaaa acaaaatcaa cgttatttat
    36961 tattgcctgt tcaatgatat ttgataaaac ggtcagattc gtcttcaata gtttttcctg
    37021 tacagtttta tcaagagttg tcagtccttc aaatgaccga tccatatgta aatctgccgc
    37081 atggataaat ttcaccatta aaaatttcct cctttttttc ttttatcata ccattttcaa
    37141 tgtttttttt catcaataac cgaaaatttg ttcgtattat gcaaagaaat tacgataaat
    37201 ggccgacttc aaaaaataag tttattcgaa aaaaagattg tgacatgatt tttgtcacaa
    37261 tcctaaatat ctatgttata ttcgcttcgt ttgacactga agaagtacta tttgtactgg
    37321 aatttatgct ccatttttcc gtatttagaa ttctatgttc tatactacct taattagttt
    37381 atatggtaag aattctttca aatgcttgag atactccgtc tttatcattt gaaacagtga
    37441 cataatcagc ttttttcatg atttcttgat gactatttcc cattgcaaaa ctaatatctg
    37501 cactttcaaa catagataag tcatttaaac tatctccgat cgcgattctc aaaggtttct
    37561 ttaaattgta gattgattcc aaacgatcaa acaccacacc tttagataca ttttgaggta
    37621 cgatttcaat atttacaggt gccgattcag taacagacac atttagcatc tttttcaaat
    37681 ggttaaatat attagctttc aattgacgat taggaataaa aataaaaaat ttgtaaatgc
    37741 tgatagcatc cttttctata
//
LOCUS       DAZUKC010000021        37368 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000021 DAZUKC010000000
VERSION     DAZUKC010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.021"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..963
                     /locus_tag="ACG80T_000991"
     CDS             <1..963
                     /locus_tag="ACG80T_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen binding domain-containing protein"
                     /protein_id="HFS4840659.1"
                     /translation="PKRGNPAGAVFELQNREGETLQTGLTTGEDGKLAIDGLAPGAYQ
                     LVETQAPTGYELDATPIEFEIERSQTAVVELTKANRLTPGGVVLTKIDDQSGEVLQGA
                     VFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIER
                     SQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGK
                     LAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRG
                     NNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(1316..2164)
                     /locus_tag="ACG80T_000992"
                     /pseudo
     CDS             complement(1316..2164)
                     /locus_tag="ACG80T_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2317..3429
                     /locus_tag="ACG80T_000993"
     CDS             2317..3429
                     /locus_tag="ACG80T_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS4840660.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3507..3800)
                     /locus_tag="ACG80T_000994"
     CDS             complement(3507..3800)
                     /locus_tag="ACG80T_000994"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFS4840661.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3968..4282
                     /locus_tag="ACG80T_000995"
     CDS             3968..4282
                     /locus_tag="ACG80T_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFS4840662.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4303..6282
                     /locus_tag="ACG80T_000996"
     CDS             4303..6282
                     /locus_tag="ACG80T_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS4840663.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6295..6621
                     /locus_tag="ACG80T_000997"
     CDS             6295..6621
                     /locus_tag="ACG80T_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFS4840664.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6637..7155
                     /locus_tag="ACG80T_000998"
     CDS             6637..7155
                     /locus_tag="ACG80T_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840665.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7155..8507
                     /gene="celB"
                     /locus_tag="ACG80T_000999"
     CDS             7155..8507
                     /gene="celB"
                     /locus_tag="ACG80T_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFS4840666.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8591..10096)
                     /locus_tag="ACG80T_001000"
     CDS             complement(8591..10096)
                     /locus_tag="ACG80T_001000"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFS4840667.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLEFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10188..10778)
                     /locus_tag="ACG80T_001001"
     CDS             complement(10188..10778)
                     /locus_tag="ACG80T_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4840668.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10957..12603
                     /locus_tag="ACG80T_001002"
     CDS             10957..12603
                     /locus_tag="ACG80T_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS4840669.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12652..13371
                     /locus_tag="ACG80T_001003"
     CDS             12652..13371
                     /locus_tag="ACG80T_001003"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFS4840670.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13850..14797
                     /locus_tag="ACG80T_001004"
     CDS             13850..14797
                     /locus_tag="ACG80T_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4840671.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14809..15729
                     /locus_tag="ACG80T_001005"
     CDS             14809..15729
                     /locus_tag="ACG80T_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4840672.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15770..17227
                     /locus_tag="ACG80T_001006"
     CDS             15770..17227
                     /locus_tag="ACG80T_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4840673.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17572..18186
                     /locus_tag="ACG80T_001007"
     CDS             17572..18186
                     /locus_tag="ACG80T_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS4840674.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18179..19903
                     /locus_tag="ACG80T_001008"
     CDS             18179..19903
                     /locus_tag="ACG80T_001008"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4840675.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19900..21351
                     /locus_tag="ACG80T_001009"
     CDS             19900..21351
                     /locus_tag="ACG80T_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS4840676.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21824..23977)
                     /locus_tag="ACG80T_001010"
     CDS             complement(21824..23977)
                     /locus_tag="ACG80T_001010"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFS4840677.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23979..25025)
                     /locus_tag="ACG80T_001011"
     CDS             complement(23979..25025)
                     /locus_tag="ACG80T_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4840678.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            25175..26464
                     /locus_tag="ACG80T_001012"
     CDS             25175..26464
                     /locus_tag="ACG80T_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFS4840679.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            26477..29179
                     /locus_tag="ACG80T_001013"
     CDS             26477..29179
                     /locus_tag="ACG80T_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFS4840680.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            29164..30021
                     /locus_tag="ACG80T_001014"
     CDS             29164..30021
                     /locus_tag="ACG80T_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS4840681.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30092..30949)
                     /locus_tag="ACG80T_001015"
     CDS             complement(30092..30949)
                     /locus_tag="ACG80T_001015"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFS4840682.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            31160..33331
                     /locus_tag="ACG80T_001016"
     CDS             31160..33331
                     /locus_tag="ACG80T_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFS4840683.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            33399..33740
                     /locus_tag="ACG80T_001017"
     CDS             33399..33740
                     /locus_tag="ACG80T_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFS4840684.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(34016..35770)
                     /locus_tag="ACG80T_001018"
     CDS             complement(34016..35770)
                     /locus_tag="ACG80T_001018"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4840685.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLSFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(35781..36617)
                     /locus_tag="ACG80T_001019"
     CDS             complement(35781..36617)
                     /locus_tag="ACG80T_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS4840686.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(36619..37302)
                     /locus_tag="ACG80T_001020"
     CDS             complement(36619..37302)
                     /locus_tag="ACG80T_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFS4840687.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT        12056 a         6577 c         7674 g        11061 t
ORIGIN      
        1 ccaaagcggg gaaatcctgc aggagctgtt tttgagttac agaaccgaga aggagaaacg
       61 ctacaaaccg gtctaactac aggagaagat ggaaaactgg caattgacgg tttagcacca
      121 ggagcatatc agctggtaga aactcaagcg ccaacggggt atgaattaga tgcgacaccg
      181 atcgagtttg agatcgaaag aagtcagaca gcggtggtag aattgacgaa agcaaaccga
      241 cttacaccag gcggtgtcgt gttgacaaag atcgatgacc aaagcgggga agtcctgcaa
      301 ggagctgttt ttgagttaca agatgccaat ggaaccgttc tgcagtcagg tctaaccaca
      361 ggagcagatg gaaaactggc gattgacggc ttagcaccag gagtgtatca gctggtagaa
      421 acccaagcgc caacggggta tgaattggat gcgacaccaa tcgagtttga gatcgaaaga
      481 agtcagacag cggtggtaga attgacgaaa gaaaatcgac ttacaccagg cggtgtcgtg
      541 ttgacaaaga tcgatgacca aagcggagaa gtcctgcaag gagctgtttt tgagttacaa
      601 gatgccaatg gaaccgttct gcagtcaggt ctaaccacag gagcagatgg aaaactggcg
      661 atagatggct tagcaccagg agcatatcag ctggtagaaa ctcaagcgcc aacggggtat
      721 gaattggatg cgacaccagt aactttcaag atcgaaaaag aacaagaagt ggcgatcttt
      781 ttaacaaaag agaatagaaa agttgctgat agctcagata taagaggaaa caatccaaca
      841 agcagtggaa ataagcatct tccaaaaact ggagaaacca tcattagcca gtttatattg
      901 tcaatattgg gtgtgctact tgttttcatc agtataaaat ttagaaagaa acgaaatata
      961 tgagctaaaa taaaaaattg aataagcata gtttttaaaa tcaactagta cccatcaatt
     1021 gaatggaaac taccgatttt agaaggggta tacaaacaaa tattagtatc ggagtagaga
     1081 aggttgaacc caaccagtaa ataagtaaag acaattttgt gctgcgttga gaagtgaaaa
     1141 ataaaccttt gtagtaatac tagttaataa aaaatataca aaaacactgc gaaaatcatt
     1201 cttgagtttc gcagtgtttt ttgcctaatg actcaagttt attgggtaat actttgattt
     1261 tctttctaca aataatcagc gatcagttgt aattttttct ctagttttaa tggctctttg
     1321 tcaataagga gaaaaacaag tcgggtcttt tgtctcggaa agcttcaacc aactcgtgaa
     1381 taaaggttac gcctccttga aaggcgtgga aaaaccaagt aatcgatcaa tcaccaattt
     1441 cgcatttggt agaacatgtt tggtgagctg aaagtaggtg gcgttcatgt ctgtaactaa
     1501 gaattctatt tctttaggat tagtgcagct aaggaaatag tttgttaatc gaggaatttt
     1561 ttgtgtaggt aaattttctc gattaatttt tctgtctcgc catccgcgga aataaaactc
     1621 agtttatcct caacggaaac gtgagaacga aattcacaac cctcaacact caagacagaa
     1681 ttcttttgga agactttggt aaatagcttc tcaactcttt caatgtgcga ataacggttg
     1741 tcaaagatac ctaacaactt ttcgaaataa aaaataacga cactttttca gtcagtaaag
     1801 aagtgatttt atgtctgaca tgatttgaaa tcgagtgcct tggacggata aaataacttt
     1861 gagcgttcca atggcttcga cagtttttac aggtatagcg ctgctttttt aggcgtataa
     1921 ccattggcat atgattatat tattcaaagt ggacaatggt ttccttcttc ttgtttttca
     1981 ccaccattaa tttcctattt ccatctacca cagcagaacc acaatttcta cagacacatg
     2041 gagcagggaa gagaactgca gcaacgatca atgttttttt cttctgtaag gtttcgtaag
     2101 agacctctgt aatcatcaaa tttttgtcta ttgatctcag aatttttttg atagaaccat
     2161 tcataaagca tatcgttctc tcagttgttt attttgtggt gatttaatca tacaagaaaa
     2221 cgatatgttt ttttataaaa acatgaatag gaaaaatagt tgacaagaaa atgacaacgc
     2281 catatgatgt tccatagtat ttacaacctg tcatagatga ctgtaaaaaa taaaagtaaa
     2341 aagaaaggac gacaacaggt ggatttttat cagtctctac aactagatcc gtttatttta
     2401 aaacaaaaaa tacgagaagc agcttctaaa aaagaaaagt gcatgtatat ttgttcattg
     2461 tttttacgat cattattcat cgtattgttt gccatttgtt ttatcattat aattacaaca
     2521 ttatttgaat cgaagcataa accatacgct gtggttttat tctgtatgtt gatgagtatt
     2581 cgttttgttg actttggtta taagatttcc cattcgatca taggtttagc aatcgtcatg
     2641 ttctcactat tagtagcacc gtatgtccag ctgatcaaat ggtcagttat gggcatgttg
     2701 attcatttta ttttactaag cagcatatta atggcaaccg ctagtgatcc caaaatgggg
     2761 aatgcttctt tgtacggctt ttcatatctc tttattgttt attcgttacc taaagattcg
     2821 ttgaacaaga atttctttac acaaacaagt agcttattat ttttgttttt ttgttggttt
     2881 tcagttcttc tttacagaaa acatcgagaa aagaacaaag acaaaagctt gtttaaagag
     2941 atttttttga aaggcatgta ttcgcaggaa aaaatttgga tgctcatcta tgcatttggc
     3001 attagtcttt tgatcgttgc aggagagtat gtcccttttc agcgattgat gtgggcaggg
     3061 tttgcatttt cttctattgt atctagctat ggattgatgt cgattggttt caaagaacga
     3121 gcagtagacc gaatcattgg ctcattgatt ggatgtgttt tatttattgg tatcagccaa
     3181 tttattcctt ttaactgggt cggtatatta ggtggtctag ctttaggtgt ttgttctacc
     3241 tatagatata aaacagtttt taattctttt ggtgctttgg caatcactgc cagtttattc
     3301 ggggttccag gagcagtaac gatccgcatt tttgagaata tccttggggt atgtttagga
     3361 atcatgtata ttggggtgac tgaaatactt atccgaaaaa taagagaaaa acacggtttg
     3421 aatcattaga tgagatcgaa taaaaaataa ggaaaaagtg aagatacaca tggatgataa
     3481 attgctatca ttccatgtgt atctttttac ggtaaatcag tgaaacgttc gactttcatt
     3541 tttaatttat actttttacg gagttcgttt atttgagcca tcatttcgga tttgtgatgc
     3601 ttttcgattg cttcttcatc tttccaacgg tcgatcagta aaagagattc tggatcttct
     3661 agtggaaagt aatagtcata tttctcgttt ccttcttctg cacgaatcgc tttgacaatg
     3721 tttctttcga ccatttcttc tgcaaatttt ctggcttgtc catttgtact tgtatacaaa
     3781 atattcactg tgatactcac taaatcccct cctgaaaaaa ttctatcact agtatatcac
     3841 ggtgccagaa attattattc aagatatgat acacattttt tccgcaatgc tacggaaata
     3901 tatattttga atagaaaacg cttacaagat atgcttggtt tataaaaata aaaggagtga
     3961 ttgatttatg aaaaaagcat tgattatttg tgcggcagga atgtcatcct cgatgatggc
     4021 ctcaaagaca acggatttct ttaagcaaaa aagagaagag gtactcgtag atgcagtttc
     4081 agctacagaa ggcgataata tgattaagaa tagtgatttt gatttatttc tgatcagccc
     4141 gcaaacgacg atgtttttgg ataaattcaa aaaattagga gaatctgttg gaaagcctgt
     4201 tgttagtatc cctttccaag cgtatgtccc aatccctaca ggcatccaga agttagcaga
     4261 ggtgatcgaa gagaacatat gatggaatag gagtggatga taatgaaaca gaaggaaaaa
     4321 ttgctgttga gatatttgat tgatcacaag aaggagtatg tcaccagtca acgtttagct
     4381 tctgaattgt tattatctga tcggacaatc cgtaattact tgcagcggat caaagagtta
     4441 gtagaaaaaa acggaggtaa gatcattgct aaaccaggtt atggctacca actgcatatt
     4501 ttacaaagac tgacatttga cttgtttttg agtcggcaag aaatagtacc tggatatgct
     4561 agagaaccgc aaggatttta tgagtccgaa gatcgacaaa agtatatatt gaaccaattg
     4621 cttttggaag atgccattct ttttatggat gatttggcag aagagcttta tatcagtcgt
     4681 tccagtttga caaaggacat gcaagaaatc aaggagcgat tgattccata ttcactaaaa
     4741 atcgtttcta agcatggtca agggacatgg atcgaagggg aagaaagaaa tcgacggcat
     4801 tttatcatgg atactttttt tggaaaccat tatggaaatt cattgaaaga atatttaggg
     4861 caaagccgtt ttttcccaga catcagtttt gaagagctag tcattatcat tctcgatgaa
     4921 acaagagaag ctaagttaaa agtttcggat tttatcatac agaatctgac tttacacttg
     4981 gctttgggga tcaaacggct ccgagaaggg tttgaaatca aggaattagg catcgaacaa
     5041 gaagtttcta agcgagtaga gtatcaagtg gcgcagcgga tcgttcaacg tattgaggta
     5101 atcgcaggtg tatcgtttcc cgaagaagaa attgcttatt tgacattaca tctcatggct
     5161 aagtcgaatc acaaagaaaa tcaaaatgat caggaactat caaaggaatt agcgaaagtt
     5221 ctgaaaagat tgtctcagga atttcatttt tcattagtag aggatctcca gcttttcaat
     5281 ggactactcg atcatttgtc accgatgatg attcgattga gccgaggtat tcgcattgag
     5341 aacccattga ctgaggagat aaagaaagaa aatcccaaag tctttgacgc agtaaaaagg
     5401 catttcagca acatgccggc tttgaaaaac tataccatca atgaagatga atgggcctat
     5461 ttggctttgc atctcatggc ggcattggaa aaagaaagag ctgcgcataa actacatgca
     5521 ttgattatct gtgcaacggg ttacggcagt gcccaactat taaagaatcg cgtagtcagt
     5581 gaattcggta aaaatattac agtggtaagt gtaaaaggct attatgagat caatgaaaca
     5641 actttaaaag gaatcgacct gattatttct tctattgatc tttcgacaat gtttttcaaa
     5701 attccagttt tacatgtcag tatttttttg aatgaagatg atgttcgtaa aatacgaaag
     5761 gtagttggag aatctatacc ctatcattcc tcagatagac cgataccagc attttctcta
     5821 cagcaaaaaa agcagattta caccgaacag ttgtctgaaa aatttttcaa gacttatacg
     5881 tacgaaccga aaaaggaaga aattcttcat gatttgctgc aaaagctgag cataaatgaa
     5941 gaagaaggat atataagaga aatgcgaaag cagatccagc aacgtgaaaa tatgggacaa
     6001 atcatattca gtgatacagt ggcagtccca catcccgctg ttccagttgg aatcgaaaca
     6061 aaggtagccg ttgcattgat tccttctggg atgcagtggg atcaatatca agaaattcat
     6121 tttgtttttt tagtttcgcc ttcttatgtc gaaaatgaag gaattacggt cgtgactaaa
     6181 gcaatcgtta ggttagttga ccagctaaaa gtacagcagg aaatcttagc tgaacctatt
     6241 tttgagaatt tcagcagtaa atttatcaaa ctgatcgaat aaggaggaaa acagatggct
     6301 gaaaaaataa acagtgaaac actacaagtg acttcttttg atatcatttt ccatagcgga
     6361 aatgcacgga cactgataca tgaagcattt cgctcgatgc gtaaagagat gtttgaagaa
     6421 gtagaagaga agttgcgaca agcaaatgaa gagattcttc atgcgcatca gtcacaaact
     6481 caacttctga aagaatatgc cagcgggaca aaaatagaaa tggaaattat catggtccat
     6541 gcacaagatc atctgatgac gacgatgaca ttattggaag ttgcacagga aatgaattat
     6601 ttgtatcaaa aaaataaata gaaaggaagg atcattgtgc aggatacagt aaaaaggaaa
     6661 atcgaattag aaaaaaagca acttagtttg aaaaacatgt atttcaatcg ttatctaatc
     6721 gtacgttacc tgactgcctt tttctttttc atgaacatgc aatggatgat tctgcttcta
     6781 tcagctaaaa gtttaggaag tagtttaccg atgattctga ttttagctat tttgcctgca
     6841 gtaggagaac aagtaaagct ttatcgaaaa caccaaacga acgttccatg gacaaagtgg
     6901 tattttttgt tccagagtgt ctttaattta ttagtgattc cagtgttgtt tacctcagga
     6961 tttacattct tgtatccttt tatggcaaat aacaatggag gacagttatt tgttttcatt
     7021 cttgttgtaa gcggtatttt tgccagtgca tttatacagt atcgtttaaa gaaaatttca
     7081 ctcaaccaag accaacagta tatccgtatt aaacaatatg aaaaagcgtt atatttagga
     7141 aaggagaata actaatgttt gattttttgc agaaatacct gatgggacct atggggaaaa
     7201 ttgcgcaact taaaatcgtt cgggctgtca tggcagcggg gatggcttct attcctttca
     7261 cgattgtagg ctcgatgttt ttggttttaa atattttgcc attaccgttc ccatttttag
     7321 aaggattttt taatgcaact tttttcaaag ttagtgactt gtacatgatc gtcaatacaa
     7381 tgacgatggg gattttatct atttactttg ccattgtgtt tgcttatgaa ttaacgtcaa
     7441 ttgaacgaga tgaacaaaaa ttaaatgtta atccgctcac gggggcatta ctatctgttt
     7501 ttgcattctt tatgtgtatc cctgaattga tcatgactga tggtaaaatc agcttgattg
     7561 cttctatcaa tgagacagaa actgtagtaa atggtattag gatgggctct tttgtcgaac
     7621 gtttaggaac ttcaggaatc tttacagcta ttattatgtc gattattgcg gtcgaattgt
     7681 attgtatgtg tgtcaagaga aattgggtca taaaaatgcc agatgttgtt ccgccaggag
     7741 tttctcgttc tttcactgct ctgatcccaa catttgtcat tgcgtttgtg gtgatgatca
     7801 tcaatggatt attagttgca ctaggaacgg atatcttcaa aatgattgct atcccatttg
     7861 ggtttgttac gcagttgacg aatacatggc ttggtgtcat ggtgatttat ttcttgatcc
     7921 atgcattgtg gatcgtaggg atccatggag cgaatatcat cacttctttc ttgaccccga
     7981 tcgtattagc taatatggca gctaacgcac aaggcgcaaa ttatccgttg gcaggagaat
     8041 tcaacaattc ttatgtaacc gttggtggat caggtgcgac tttaggattg atcatcttta
     8101 ttgcattcct cgcgaaatct gatcagctga aagtgttagg aaaagcctca cttgtaccag
     8161 gattattcaa tatcaatgaa ccaatcattt tcgggatgcc aatcgtctac aatccctatt
     8221 tagcggttcc gtttttctta gctccgatgg cttctgctac actcgcttat tttgccattc
     8281 ggctggaaat cgtcaaaccg atgcttgcac agatgccgtg gccttcacca gtcgggatcg
     8341 gagcatttgt tggtagtggt ggtgattgga aagctgctgt gttagcagtc atttgtgcaa
     8401 tcatcgcatt cattatctgg ttcccattta tcaaattcta tgataataaa ttattaaatg
     8461 aagaaagaga aaaggcagca gaattagcta gtgaaaagtt aaaatgattt ctaagaaaaa
     8521 aggaactgtg acaaaaaatc acagtccctt tttttcttag aaatgggaaa ccactagtat
     8581 ataaatacca ttattcttca atcaaccgct tcacgattgc ataatcccct tcataaggtt
     8641 catctacacc attgattttt tgatagggat ccacgccttt tcccgcaata acgactgaat
     8701 cttgcggagc gctttctgct aaagcgcgtc gaatcgcttc aggacgatcc atttcataat
     8761 gaacgatcaa atctggatta gtaattgctt cattgatttc ttccgcgatt tttttcggat
     8821 cttcaaatgc tgggtcatct gccgtcaaaa aggcgatgtc agctgtttct gatagcactt
     8881 ttccaaaatc atgtcgacgg gagattgctt tgtttcctgg gctgcctaat acaacaatta
     8941 cacggccatc tggatgttca tttttggcaa attccagcaa tgttttcaag cttagataat
     9001 tatgtgcata gtccacatag acatgcgctc cattggattg gatcaattga tccattcggc
     9061 ctggtacacg agcttctttt aatccgtctt gtgcatcttg actggaggca cctgttagtg
     9121 ctgcggcaat gatcgcactg gctgcatttt cttggttgaa tcctccagcc agcaaaatat
     9181 cataagcagt atcgcagata gctaagcgat cttcatgaga agataaagta aaaccgtgag
     9241 tacctttatc tgaacggatc acttgataat tgctttcttc cgaacgacca taagtgatcg
     9301 ttggaatctg gaatagatcg gctgtttccc taagtaaatc aaaatagtca ctttcatgat
     9361 ttaaaacaac tgtttttgaa ttcaagatca attgtcgctt gcaatagaaa tagtcatcaa
     9421 atgtcggatg ttcgatcgga ctgatatggt caggcgagat attcaagaag attccgacat
     9481 ctaaagtcaa gccgtatacg cgttgtgttt tgtaagcttg tgaagaaact tccatcacta
     9541 gatgagtcat accattttca actgcttctg acatcatacg atataaatct aatgactcag
     9601 gcgttgtcaa atgggatttg aaatatgttt tgccatctaa cgttgtattc atagtagaaa
     9661 gcaatgctgt ttttttattt gttgtgtgat ccaagattgc ttttgtaaag tacgcagcag
     9721 ttgttttacc ttttgttcca gtgaatccga tcactttcaa tttattttgc ggatagtcgt
     9781 aaaaagccat agcaagaagc gccattgctt tacggatatc cgttacgatg attcctactg
     9841 ctgaattttc gtatggttgt tctgatacat aatattgaat accctgttcg attgctgaat
     9901 ccaaatattc ttctttgaaa ttcattcctt tacagaaaaa caaagtagaa gcatcagctt
     9961 ttcttgaatc ataacttaac tgaagaaatt cagctttttc tggtacatca agatgccagc
    10021 ctcttgttga aacaaattct tttaatagat tttcttttaa tagtaaatga cggatgtctt
    10081 ctaaagagaa gctcattgat tgatccctcg ctttattcaa tttcttggtt tattatagca
    10141 cactcttttt aatatatcgt ttgtttccgc tatgacaaaa tccagcttta ttcaaatttt
    10201 cttgacacag gggacaagcg gatgagtatt ttcataaaca atgaactgac tgtaatagat
    10261 aggagaacga atagtgtctc caacaatcca aaaacataat aattcaaaaa gacgataaga
    10321 ctattggtaa gtaaaagtcc ttgcggcatt ggaagccgaa aaaatttgtg gaaaataatc
    10381 ggcggtatcg ttataccgcc atttgaggcc ccgactgtgt acaatgtgta aaccccaaga
    10441 ctgaacaaaa gacttcctga aatcagtgaa aagaaatgtg aggagatcat ttccactttt
    10501 ggaacaatct ctaaaacaac aggaaaaagc agacttcccg ctacagtctt aaaaaatact
    10561 gattttccta ataggacagc ggctagaagc agcatcataa gattgatgct ccaaacagta
    10621 actgagcgtt ggatagaaaa ggccgtttca actaaatgtc cgatggcgcc cactccggcg
    10681 gaagctatat cgtgagggag caagaaccaa ttcaatccaa ctgtgataaa gaaaagacct
    10741 aaaaatataa cgggtagttt ttttatttct tgtgtcattg atatctccta taaatgttag
    10801 ccataaaagg ctgtatattt ctttacttta ccaaaaaaac gaaaccgaat tcaaaaaaac
    10861 aattgttctt ataaaagtta tataaaatcg attgccagtt tctgttttac caattaaaat
    10921 gatagtaatg ttttgtagaa agttggaatc tgaccgatgg ataatcaaag aaaaccagca
    10981 ttagatcaat taacaccgca agaaaaaatg gcacgaggct ctgcttggat gaccgccagt
    11041 aatatcattt caagattgct aggcgctgtc tatatcattc cctggtatgc ttggatgggc
    11101 gaaaatgcta aggctgccaa tggattattc aatatgggat acaatatata tgcattgttt
    11161 ctactgatct ctacagcagg aatacctgct gcgattgcaa aacaaactgc acgctataat
    11221 tcattgaatg aatatggcac ttcacgacga ttatttatcc gtgcattaca gatgatgggc
    11281 gggctaggcc tgttgtttgc tttgttcatg tacatcgcct ctccttggct agctcatgct
    11341 tctggtggcg gagaagaact gattccaacg atgcgttctt tgagtatcgc tgtattggtt
    11401 tttccttgta tgagtgtgat acgagggtat tttcaaggca atcaagaaat gatgccttat
    11461 gcgctgtcgc agattgtaga acaggtggca cgtatttttt atatgctatt ggctacattc
    11521 atcatcatga aagtcatgga tggtgactat gtgacggcgg tcacgcaatc aactttcgcg
    11581 gcatttatcg gtatgctagc aagtattctt gtccttttgt attttttgaa gaaacatcaa
    11641 gcgtatacat cggcgtttat tcactacagc gaaaacaaaa taaatattgc aacgaaggaa
    11701 ttgttgttag ataccgttaa agaagcgatt ccgtttatta ttgtgggttc aggggtaacg
    11761 atcttcaaat tagtcgatca gttcactttt atgaagatca tgtctgcatc caccgagtat
    11821 tccaatgcac agttactgga tcttttttcg atttttagtg ccaatccaga taagttgacg
    11881 atggtagtta ttgcattggc aacatcgatc gcgtctactg ggttaccgtt gattacagaa
    11941 gctgttacgt tgaaagatcg agtaggtctg gcaaaattga caagcggaaa tttacagttg
    12001 ttttcatttt ttatgtttcc ggcgacattt ggtgttatgt tacttgccta tccgctaaat
    12061 acattatttt atacaccgga ttctctagga agtaatgtac tgatccaagc aagttttgta
    12121 ggactcttcc taggactata tatgctagtt tccaacatgc tgcaaggaat gtttgaaaac
    12181 aaggcggcaa ttaattattt ggttattggg tttctagtga aactccttct tcaataccca
    12241 gccattcgtt tatttgaagt atacggccct ttattggcga caatgatcgg atttgctgtg
    12301 tcttgtacct tgatcttaaa gagaatccat gaagtagccc gctttaaacc aagttttgtc
    12361 tggcgcagga cattgctgat ctttattctt accttgatta tgcttttggc ttcgtggatc
    12421 acaaagatga tttttggtgc ttttttaaac gaagatagca aattccagtc tctattgttg
    12481 attcttgtag tagcaggtat aggggcttta gtgtatggtt atttagcttt gaaaatccgc
    12541 ttggcagaaa gactactagg accaggaata gcgcgtttac gacataaatt aaaaataaaa
    12601 taagtgaatc agaagagcta gattgatgct cttttgaaat gggggaaacg catgcgttta
    12661 gataagtttt tagcagatat gggacttggc agccgcaaag aagtcaaagg attgatcaaa
    12721 aaaggaacga tcgaagtcaa tggacaagtg gtgaaaagcg ataaagcgca agtaaatgaa
    12781 ttcgaagatg cggttactta tttaggagaa atcattacgt atcaaaaaga tttttactac
    12841 attttgcata aacctgcagg agtcatttct gctactcagg ataatcatga tcaaaccgta
    12901 atcgatttat tggaagacca ggattatcgg gaagatcttt ttccagtcgg ccgattagat
    12961 aaagacacag agggcctctt gattttaacg aatgacgggg tgtttgccca tcagttacta
    13021 tcacctaaaa aacatgtgga gaaagaatat ctagctgagg ttagcggtat aatgacagaa
    13081 gaagatatcc gtttatttgc agaaggactg attattgatg gcgatgagca gacattgccg
    13141 gcaaaactcc aaattgatca agtagatcaa gagacagaaa cttcaattat ccgtctgatt
    13201 ttgcatgaag gaaagtttca ccaagttaag cgaatggtca aagcagtagg aaaagaagtc
    13261 ttatatttaa aaagaatccg tatgggaaaa ttttggctgc ctaaagattt gaataaaggg
    13321 caatatcgac ccatgaccaa agaagagcta gaacaagtga gagaaaaata attgaatgga
    13381 tagctaaaag aataagcaga gtagttgtag cgccatctga aaagcagact tgtccgtctg
    13441 actttcggat ggcttttttg tgaaatttcg gcttatttcc gtagaagcta cttctggaac
    13501 accgtttgtt cggaataagg gactgtgaca agacttttgt tacagtcccc attgttcctt
    13561 gtgactcaga gttagatagt aaggacacga tctccctaat actcaggcca aaacgctgaa
    13621 ttcacaacct ctgataaacg gtgttcaatc agctgaccct cggtattagc tcaaaaaacg
    13681 gcaaaatatg aggaactatt ttcccgtttt tttctctaat actcaggcca aaacgctgat
    13741 ttcacaacct ctcctatact ttgtttcatt ttccctatac tttgtttatt tattctcatt
    13801 attgaaagcg ctataattac gttgcgaatg aatcgaaagg ggtttttaga tgaaaaaacg
    13861 aaaggggttc aatcattttt ttatgaatct atggcgttat aaagcacttg tcctgatggc
    13921 tgtaccgggt atgctttgga tgattttttt cttttacata cctgtaatgg cgaatgtcgt
    13981 agcattcaaa aattttcata tttcagcaga tgggttcttg gctagcttga gggaaagtcc
    14041 ttgggtgggt tgggaaaatt tcaaattttt attttcttcg gatcaagcat ttctcatcac
    14101 taaaaataca attttatata atgtagcatt tatccttttg aatctgctga tctcagtcgt
    14161 atttgcaatc attatgagcg agttgagaaa taaacgactc gttaaagttt atcaaacgat
    14221 gtccttacta ccttattttc tatcttgggt cattatcagt tactttgttt acgctttttt
    14281 aagtcctgac aaaggaatat tcaatcagtg gatcactgga catggaggag aagctgtcaa
    14341 ttggtataat gaaccgaaat attggccgtt tatcttggtg ttcattggta catggaaagg
    14401 gattggttat aacagcatta tttactttgc atctgttatg ggaatcgatc cgacttatta
    14461 tgaagctgct atggttgatg gagctagcaa atggcagcag atcaaacatg tgacgatacc
    14521 acaacttatt ccactaatga cgattttaac gattttagcc gtaggaaaca ttttccgagc
    14581 tgattttggc ctgttttata acattcctag gaattccggt gcattatatg atgtgactca
    14641 agttttggat acgtatatct acaatggcct gacatccaca ggagattttg gtatgacggc
    14701 tgctgcagga ctttaccaat cagttgtcgg ctttgtcttg ttgatgatca caaatacgat
    14761 cgcccgacgt tttgatagcg attctgcatt attctaagga ggaaaaaaat gaaaaaaaag
    14821 aaagtggaga gagtccaagt ccgctctttc aataagacgg ctaatttttt ctctaatata
    14881 atgattgcac tctttgctat ttcctgtatt ttgccattta tatttgttat cgccatctct
    14941 tttaccgatg agtccatatt gtcgactcac gggtacagtt tttggccaga aaagttcagt
    15001 atatttggtt atacgtactt gttcgaccag atgcaggata aaattttcca agcgcttttc
    15061 gtcactgtac ttgttactgt tttaggtaca ctgatcaata gcacggctac ttctttatat
    15121 gcttatgcga tttcacggtc aaactttcca ttccgtcgtt tttttaccgt tttttgtttg
    15181 attacgatgt tgttctcacc agggatggtg gccaattatc ttgttatgac aaatcttctt
    15241 cagttaaaag atacgatttg ggcattgatc ttgccaatgg ctgtcagtcc cttcaatatt
    15301 attgtgatgc gcacattctt taaacgttcg gtctctgatt cgattattga atcggcacgg
    15361 atcgatggag ctagcgaact gcgaatattt gttcagatcg ttttaccgtt agctgttcca
    15421 ggtattgcga caatcagttt atttgccgca ttgggttact ggaatgactg gttcaatgct
    15481 ttactataca tccaagatga taagctagtt ccactacagt atcttttgat gaaaatccaa
    15541 agtaatattc aatatctgac acaaaattca ggagcaggaa gccaattgac aggcggatta
    15601 gcctcagttc caggagaatc tgccagaatg gcgatcgtag tcatctcgac gttgcctatt
    15661 gcgatcagct atccattttt ccagaagcat tttgtcaaag gtctaacaat cggcggagta
    15721 aaagaataaa gaaacaaaaa tataaatcat aaaattagga ggaagaaaaa tgaagttatg
    15781 gaagaaacta gcctttacag gtgtttcagc gttgttgttg ggaacattag cagcatgcgg
    15841 aggttctggt tctaaagacc aggcagaagc tagcaattcg gacactttgc agatgtatca
    15901 aatcggagat aaaccagata atttcgatca attgatggaa gtagcaaaca aacgaatcga
    15961 aaaagagatc ggtgtaaaag tcaatatcaa ttacattggc tggggcgatt acgaaaagaa
    16021 aatgaacgtc atcatttctt ccggggaaaa ttatgatatc gcctttgcga acaactatgt
    16081 ttccaatgcc caaaaaggag cctttgctga tttgactgaa ttagcgccta agtacgctga
    16141 aaaagcttat aatgatttag atgaagcata tattaaagga aatctggtca atggaaaatt
    16201 atatgctttc ccagtcaatg gaaatgtctt tgctcaacaa gtacttactt ttaacaaagc
    16261 tttactagat aaatacgatc tttcaattga tggaatccaa tcttacgctg atgcagaaaa
    16321 agtcttgaag gaattccatg aaaaagaacc aaacacggca gcttttgcca ttggacaagg
    16381 cttcaaagtc caagggaact ttgattatcc tttagggaat acgttaccat ttgctattga
    16441 tctggatgga gatgattcga aaatcatcaa ccaatacgat aatgaaacgt atatcggact
    16501 gttacgtaca atgcatagct ggtacaaaca aggactcatt ccaagtgacg ctgcaacgag
    16561 caataaggat tatccactag aaggaaatac ttggctaatg cgtggagaaa cacaaggacc
    16621 gtatgattat ggcgatacaa ttttgacaaa tgcagcaaaa caagaacttg tctcaaaagc
    16681 aattactgtt ccactaaaat ctacagctca agcacaaatg gcaaattttg tcgtatcaaa
    16741 tacttcaaaa aataaagaaa aatcagtaga acttctaggg ctgcttaaca gtgatccaga
    16801 attattgaat gggcttgttt gggggatcga gggagaagca tgggaaaaag tcgatggttc
    16861 agatgataaa atcaaattgc tagatggtta tcagccaaat acacatatgt ctgcttggaa
    16921 tacaggaaac aacaagattc tatatacaca agagtctatt acggatgaca tgattgccaa
    16981 acgtgatcaa tcgattgctg atgcagaaac ttcaccgatt ttaggattca gcttcaacac
    17041 tgacagtgtc aaaacagaat taagtaatat ttctaatgtc atgaatcaat atttagatgg
    17101 attgaataca gggactgttg atccagatga aacattaccg aaattgaaag acgctttgga
    17161 caaggctggc tatgataagg tattgaaaga aatgcaaaaa caatatgatg agttccgaca
    17221 agaataataa tgaataaaga caaaggggtt gtgacaaaag acgtgtcaca acctcttgtt
    17281 ccgaataaac ggtgttcaaa agccagtttc tcggtattaa gctaaatata tgtaaaaatg
    17341 tggaaagcca tattcacaaa atttttctta ctactcgaaa ctaagcggct atttcacaaa
    17401 ctcttggtaa acggtgttca atcagctgac cctcggtatt cgctcacaaa acgacaaaat
    17461 atgaggaact atttttccgt ttgtttctct aataatcagg tcaaggtgct gattgcccaa
    17521 cctttttttg tataatgaga aagtataaac ttaatgtttg gaggagaaga tatgttaggg
    17581 aaggttttgg aagagttatt tatccggatc tgggtcatta taaaactaac cttgtatttc
    17641 tggatatata catttgccgg cgggatcatc tttggattag gtgcggcatg gaaaacagtc
    17701 aatgaactat tttatttgta cggattcgag tataaagaaa tcacaataaa aagaggctgg
    17761 aatatatata aaagaaattt tttaagaggc aatctgctat ttagtttatt tttatctggt
    17821 actgctttat tgagttacaa tttatattta tctgttcaaa tcaaagggct cattttttta
    17881 gctattgatt ttattttact ttttgccttg ctctgcctat ttgctactta tcaatacagt
    17941 atgattttag atagtgaata tacgatctct gttcctaatc tgctaaagct aagttttatt
    18001 tcggtttttt ccagcttttc gtcttttcta aaaataatta ttggtagcgg tataatttta
    18061 ggcgtgactt ggcaattcaa aggattgatc ctgtttggtg tcattggtct tttgacggtt
    18121 tggaatggta cgatgacaac acattggcgt gaagaattag ataaacagtt ggaaagttat
    18181 gaataagttt cggattctat tttcaaaagg aaccttacta aatcgtctgt tgcggaaata
    18241 cgcggttatc atgatcggtg taacgatgac tgctaccgtt atttttagta tccatacttg
    18301 ggaacaaaat caaaaacagg cagaaaacat gacaagtgat gccgttcaaa gcacaagcag
    18361 aatgctgaat gataaaacaa ccttgagtag gatcatcaaa aaccagcttg tagggaattc
    18421 cgaaaagatc gaaaatgtga cgacctactt aacaaaaccc atagatcagt atctgatgta
    18481 tgtatacgaa cagcaaaaca gtaccgatga attagtttct ttcccaaatc aaatcaaaga
    18541 tctctacgca aattatgaag aactaagtgc gatatatatc gtgttgaatc aactaccgga
    18601 gtattatgaa tcttctagag aaaacaaagg tggggaaata aaaaagggga caccaaaatt
    18661 actagacgcc ttttatatca agttgcctat cacacaagga ggagctgagt caggaagtat
    18721 ttttattggt tttcaaaaga aagaactaga tatgatttta gaaaatctaa cgtcattcaa
    18781 tggactctct ttatacatga tttctggaac gacaaatcga ctatacacat tccatgataa
    18841 taaaatagct aaagaaacgt ttgaaagaca agaaatgatc atcacagaga gtctgaagaa
    18901 aaattcacaa ttgccgattg acgatctgga aaaaaacaat tttgttcaac accaagaact
    18961 gtctgatgat tatcgtatat tagctgtact agataaaaaa agtgtaaaaa tcgaaacggg
    19021 aaaaaatctt gccccgctga tcattggtat cattatttta gacaatatac tgcttatctt
    19081 tctttataaa acattcaaac gatattcaca gcaagtatct atcgtcatgc aggccatgga
    19141 tcaaacagca gcagggaatt tagagacgcg gatcgacata acaaacacgg aatatgaact
    19201 gaaggaactt tcaatcggta tcaacgaaat gttggatagc atcaatcagt ttgtagaaga
    19261 catctacaaa ttagaaatca aacagcaaga cgcacatatg cgggcgcttc aagcacagat
    19321 caatccacac tttttatata atacattaga atatatccga atgtatgcaa tcagcgaagg
    19381 aagtgaagaa ttagcagatg ttgtgtatgc gttttccgct ttgcttcgaa acaatacgaa
    19441 tcaagagaaa acgatcactt taaaagaaga actggacttt tgcgaaaaat atgtctattt
    19501 gtatcaaatg cgttatccta atcgagtagc ctatcatttt atgatcgatc ctgatttgga
    19561 aaagatcgaa gtacctaaat ttgtgatcca gccattagtt gaaaattatt ttaaacacgg
    19621 tattgatttt actcgattcg ataatgcgct aagtgtcaaa gtcttgcagg aaggcaaacg
    19681 ggtaaggatc atcatcaaag ataatggaaa agggatgact gaaaaacggc taaaacagat
    19741 tgaagaaaaa ctgtctcatc ctaaagtgga acttcacggt tctatcggtc tgcaaaacgt
    19801 gaatgaacgt ttacgtgcca gcttcggcag cagttattat atgtcgcttg agaataatga
    19861 gacaggtgga ctgaccgttt ctattacatt caaggaggga tgagcgtgta tcaagtgtta
    19921 ttggtagatg atgaatacat gattttgaat gggttaaaaa agataattga ttggcaaagt
    19981 ttaggctttc aaatagtagc aacagcagag aatgccaaag aaggactagc tgtacttgaa
    20041 cagcgacaga ttgatttagt agtaacggat gtgacaatgc cagaaataaa tggactggaa
    20101 tttattgaag cggctcaaaa agaacgtcat aattttgaat ttatgatttt atcaggatac
    20161 caagaattcg attatttgaa aggaggtatg caattaggcg ccgtgaatta tttgatgaaa
    20221 cccgtgaata agttcgaatt ggttgaaagc ctcaagaaaa tcaaaacacg gttagatcag
    20281 caaaatgaac aaaaaaatca acaagaaatc tatcaagaac tgttattcag tcaatggttg
    20341 aatgaagaat tggatgaaat aagtgaagaa gaaataatag gacaaataag tgagaagcag
    20401 cataggatag tattgattca actggcaaga actcatgata ctcttatcaa tagatggttg
    20461 caagaacggc aacaaatctt ttactatcaa cgaaattacg gagaccgtgt attgatcacg
    20521 ttgttgttgg aggcagatga agttgatggc ttctgttgtt ttgttcaaga aaactttcct
    20581 gatcaggaat ggctgatcag tatcggggaa gagacagatt ctttggacaa aatccctgaa
    20641 acattccaac aagcgaaaga caatctacag ttgcatcaat tttatggaga taaagacgag
    20701 catgtttttt acgcagtaca agcagaaaca aaagatcaaa ccatcgattt ttcaagtttc
    20761 agacgcgtgc tgcaaaataa acaattggaa gtcgctcaaa aaatgatcac tgattttttt
    20821 gaacagtttc agttagcagc catgatgccg gaagatatac gatattccag ttttttactg
    20881 tttatggaga ttcaacggga attgattgat ctggaagatg aggaatattt acaaggaatc
    20941 gaaaaaatcc atcaggcaaa aacggtgcaa gaacttcagc agctacttct gtcattcatc
    21001 aaaaagcatc agagacagaa aaagtacagt gacaatgtgg aaaaagtaat cgtaatcctg
    21061 caccagcatt atcaagaacc acttacactc aaagaagtgt cagaaaacct gcatttaaat
    21121 gtcatgtatc taggacagct gttcaaaaaa gaaacgaaaa aaagtttttc agcttatttg
    21181 aaccatttac gtatggaaaa agcgaaacaa ctgcttttgc acagtaatca aaacatcaat
    21241 gagatagcaa gtgagattgg ctacaacaac accacgtatt tctctaaatt attcaagaaa
    21301 atcgtcggca gatcccctaa agagtaccga gaaaacagtg gagaatcata aaaaaaaaag
    21361 ccaatgtaat gaaataactt ataaaaaatt gtctagaaat ctaattttgg gctgttctta
    21421 ttacttgagg caaggcagct ttttcatgac ctctcgataa acggagttca aaagccagtt
    21481 tctcggtatt aagccaaatt tatgcaaaaa tgtggaaagc cattttcaca aaatttttct
    21541 taatactcga aactaaacag ctttttcacg acctctccac taaatggtgt tcaatcagct
    21601 gaccctcggt attagctcaa aaaatggcaa aatatgagga actattttgc catttgtctc
    21661 tctaatactc aggtcagaac gctgatttca caacctctca ataaacggtg ttcaaaagct
    21721 tgcatctgcg gtaataagac caaccaaacg aaaaatatga ggatctattt ttgcttggct
    21781 gttcttatta cttgatgcag gacagctttt tcacaacctc ttgttaatta tagtttgata
    21841 aggaaaaagg caaatctttt gcttgatatt tcttagggtg agggatgatc cctaattggt
    21901 aagagatcgt gccgcctttc agcaaatctt gatgggtaaa atataatttc gtatattctc
    21961 tgtccatccg tttgatttta tggataaatt gttgctgcgg ccgattaggc gttgcttcaa
    22021 tcgtcaattc ctttccattt gacaaaagca aaacagcctt gtcaattaat ggcataccga
    22081 taacatattc accggttcca ggcgtgacag gataaaatcc cagactattg aagatatacc
    22141 aagcggccat acttccattg tcctcatctc cagggtaacc cattggagaa tcgtcgaagg
    22201 tctgagtcat caactgttta agcagtggct gtgccatttc aggttgtcct atataactga
    22261 acaagtaagg atagtgaaaa cttggctggt ttgaaatagc taattgtcca aattctatcg
    22321 cggccatctc actcatttcg tggatctcaa aaccatatcc tcctgggtca aacgttgggg
    22381 gctgattgca cagctcaatg agtttttctt tatatctctc actgccacca tattttccga
    22441 tcagtcctga aaaatcgtga taaacagcaa agctgctttg ccaagcactt ccttctgcat
    22501 aatcttgtcc ccaacgatta gctgaaaatg gttctctgaa cttgccatct ttatcttttg
    22561 aacgcataaa tcctgactca tagtcaaaga tattttgata gttcaatgat tgctgctgat
    22621 atttcttcgt catttcctct tcttctagta ccttggcaac ttggctaata caaaaatcgc
    22681 tataagcata gtctaatgtg tgattgactg attcgtggta gtgattagga acatagccat
    22741 atgtcaagta atcttctgtt ccttggcgtc cataatttgg atgtttgctt tgaaccgtag
    22801 cacctttttc cattgccgtt agaaattcag gcatcaagtc tcgtcggata tcttttacag
    22861 cagcatctgc aatgaccgca tcgatcaatg tcccaggcat aagtccacgt tcatcaggag
    22921 acagccattt tggcaaatag ccactttctc tatagctgtt caaaaaacct tccagcattt
    22981 cttcgtattt ttctacagca atcaaagaat aaagaggata aaccgtttta tacgtatccc
    23041 aaaacccatt atttgtatag agaacaccag gttttataga tcgactagtc gtatcatagt
    23101 ggattttttg cccactagga tcaatttcat aaaatgtttg tggaaataaa aacacccgat
    23161 ataaattatg ataaaacgtc cgcaattgtt gttgatcatg gtgtgtgagc tgtatacgat
    23221 caaaatagga ctgccattgt tcttgactgt gagcaaggta agtctctttg ctttggtttt
    23281 gttctagttc caggttataa gctgcttgtt ccgagctgat aaaagatgtc ccaaatcgga
    23341 tagtttgttt ttttgtctgt tcaaaataca acgggatcga gtcgctattt ttgttggaaa
    23401 cagctaactc atcaattgtt aaaggatatt caaatctcaa tataaaataa aaagaaaaat
    23461 caggatcttc tgaaccagca aaatgtgtga tttttccttc cactgtgtaa ggatctataa
    23521 caaagagctg atggcgtccc ggaaaggata aaagtaaacc gctgtcattt ttgctgtaat
    23581 caatagataa gattccgcca tacatgctag gtatcaatgt agaacagatg ccataacgta
    23641 attgacagat cgacagatga gtcgggcaaa atatactttc ttctgggcgg taagaacttt
    23701 gagcatggaa aagggtgttc tcctgtagtt ttccattgat cggcgtaaac agcatatgac
    23761 tgaaatcacc catccaagga cttggttgat gggtaagacg gtagccttga aaaacgcggt
    23821 catcaggatg aaaccaccag ctgccgtttt gatccgtagt ttgcggtgca aaataattca
    23881 tcccaaaagg aacgccagta taaggctggc agttcccatt agagaaattt ggctgattcg
    23941 ctgttgcatg tctagtatct atttgaccga tattcatttt atttctcctt tgttgattgt
    24001 cttttgatga atgaaactgg aaatttacta tcggatggtc gatcttttgt ctctaaccaa
    24061 gtgaccaaag catctcccgc aagcttacct aactgttcaa aatcttgtgc gatggttgtc
    24121 agtggcgggt caacaagagc tgctgcttgg atatcatcaa atccgataat tgaaaaatct
    24181 tcagggatat gataaccatg ctgtctcaag agattcatgg tctggatagc gacaacatcg
    24241 ttttcacaaa caaaagcagt tacttcatac ttagtgatat aagaaagcaa ttcatctaat
    24301 gtcgcgcttt tgtcatccag tgaagtgtgg aaagtcaaac cagctttgtc caatgcttca
    24361 aggtaaccaa ggtaacgctg tcgaacggat tgtggatgag tctgcgtacc aaacaaataa
    24421 gcgatttttg aatgtccctg ttcgatcaaa taagaggcag caagcgcacc gccttggaaa
    24481 ttatcggaaa gaacggtagg aaaaggtaac tcatagattt ttttatctaa tatgacgaca
    24541 gggaattctt tcaatgaaag ctcaaaaagt aagtcaagat gttgttctgt atcaagagca
    24601 taataaatca atccgtcaaa ttcattgatt agctcttttg ctgttttatt ttgtagaaag
    24661 tcaagcgtcg tcatcatgac atcaatctgt ttatcttgca tcacagggtt caatcccttg
    24721 ctaaaatcac caacagacaa atcagcgaca tatggaagaa gaaacagaat ccgtcgtgtt
    24781 tttgtaggag aagcaagaat ctgttccgga gaagcggatt taacgaaact gcctttcccg
    24841 cgaacgcgat aaataagtcc taattgctct aattcagtca atgcacgttt ggaagtgatt
    24901 ctgcttactt tataggtacg tgaaagttct ttttcagttg gtagttggct gtctacaggt
    24961 aattcaccag ttaaaatagc cgtttgcaaa tcattaaata ttgtctggta caatggttga
    25021 gtcatataaa cacctcttaa tgatatatca tattcaaata ataccataat ggatgaaaaa
    25081 gtaaataaaa agatattttt tgaaagcgta gacataattg gctgtatgaa gtataatctt
    25141 taaagatata tcaaatagaa agaaggtttt attagtggtt tataaagata taccaaaatc
    25201 ggtgcagaaa tttatggaag atatcacaca aaaatgtggg gatatccatg cggactgggc
    25261 aaagaatttt acagcagcat ttgccaatac tctgttgaca acagtcaaaa agcaaaatga
    25321 cggaaccacc tttttattga cgggagacat tccagcgatg tggttaagag actctactgc
    25381 acaagttcgt ccttatttag tgattgcaaa agaagatgag gaattagctg acatgatcag
    25441 cgggttagtc aaaaagcaat ttttttatat caatatcgat ccatatgcga atgccttcaa
    25501 cgaagaagct aacggtgctg gccaccaaac agatcataca aaaatgaacg attggatctg
    25561 ggaaagaaaa tatgaaattg attctttatg ctacccaata cagttggcct atcttcttta
    25621 caaaaataca ggaaaaactg atcaattcaa tcaatctttt attgaaggta tcaaaaaaat
    25681 cttggacgtg ttcaaaacag agcaagatca tgaacactct ccttatacat ttgtacgaga
    25741 cacgacaaga aaagaagata cgctgatcaa tgacgggcga ggtgcaaaaa cagcttttac
    25801 aggaatgaca tggtctggtt tccgtcctag cgatgatgct tgccgttatg gatatctggt
    25861 accatccaat atgtttgcgg tggtcgtgtt aggttatgtg gaagaaattt ttcaaacgat
    25921 actcgatgat aaagaaattg tacagcaaac acagaaattg aagaaagaga tccaagaggg
    25981 gattcagaat cacggacgaa cggttaatca aaaagggcaa acgatttatg catatgaagt
    26041 agatggaatg ggaaacgcca cagtcatgga tgacagtaat gtgcccaatc tgatcgccgc
    26101 accttacttg ggatattgtt ctgaacaaga ggaagattat ctgcaaacaa gaagaacatt
    26161 gttgagtaaa gaaaatccat atttctatga aggaaagtac gcaaaaggaa ttggatcctc
    26221 ccatacgcca gaaaaatacg tttggccgat tgcattagct atggaaggaa tgacaacgtc
    26281 agacaaatct gaaaaagagc ggatattgaa tttacttgta gcaactgacg caggaacaca
    26341 ccttatgcac gaaggattcg atgtgaacaa tcctgaaaat tatacgagag aatggttttc
    26401 ttgggcaaat atgatgtttt gcgaattagt catggattac tttgatatta gagtagaaaa
    26461 gtaggaggta tcgtaaatga aaaagaaagt atatatcatt tctcatagcc actgggatcg
    26521 tgaatggtac atggcttacg aacagcatca catgcgactg attgaattga tagacgatct
    26581 actagagcta tttgaagttg atccgtcctt caacagtttt catttggatg ggcaaacgat
    26641 tattttagat gactatcttc aagtaaggcc agaaaaaaga caggcaatcc aacaagcaat
    26701 caatgaagga aaattgagaa ttggaccatt ttatatcttg caagatgatt ttcttatcag
    26761 ctcggaatcg aacgtgagaa atatgctgat tggcatggaa gaaagccgta aatggggaac
    26821 ccctgtcatg ttaggctatt ttcccgatac atttggaaat atgggacaga cgcctcaatt
    26881 aatgaaacaa gctggtattt cagcagcagc gtttggccgt ggtgtaaaac caattggctt
    26941 tgataatcaa gttttggaag cagaaaacta ttcttcccaa tactcagaga tgtggtggaa
    27001 aggccctgat caaacagcaa tctttggtct gctatttgcc aattggtata gcaacgggaa
    27061 tgagattcca gtagaaaaag aagcagctct tgccttttgg aagcaaaaat tagcggatgc
    27121 agaacaatat gcctctacga accacttatt gatgatgaat ggtgttgatc atcaaccggt
    27181 acaaaaagat atatcaaaag caatccatct cgcaaatgaa ctgtttccag attacgagtt
    27241 tattcattct aattttactg actatctgga agcagttcaa aaagatgtgc cagaagactt
    27301 aggctcagta gaaggagaat tgacaagtca agaaacggat ggctggtata ctttagccaa
    27361 tacagcatct gctcgggttt acttaaaaca atggaatacc aaggtccagc gacagctaga
    27421 aaatattaca gaaccacttg cgacgatggc ttatgaagtt agtggaaact atccccatga
    27481 tcaattagat tatgcttgga agacattaat gcaaaatcat cctcacgaca gtatttgcgg
    27541 ttgttcagtc gattctgtcc atcgggaaat gatacctcgt tttgaaaaag cagatgaagt
    27601 tggaaagtat cttgcacaag attccttaga acagctgacc gcagcaatcg atacgaccgg
    27661 attcccaaaa gacagctttc catttgtcat tgtaaatact gcaggaatgg ataaaactgg
    27721 tgaagcagaa ataaccatcg aattagaaag aaaaagattc gctgaaggaa tacctgaaca
    27781 actatatcaa gagttagaaa atttgcctaa acgtaagtat catgtagaaa caaaaagtgg
    27841 ggccactata ccagctattt tatctgaaga aactgtccaa tttggttatg atttgccgaa
    27901 agaccgtttc cgtgtgccat atatggcaag aatgatcaaa gtgactttac cattagaaaa
    27961 catgccagca ttttcttggg aaacattcgc gcttgtagaa ggggaagcga aagcagaaga
    28021 aaaggaaaca atgattcatc agagtggacg aatcatcgaa aacggccctc tacatttgac
    28081 gattgaaaaa aatggtacca ttacaatgga agatcgcaaa aacaaacgaa agttgaataa
    28141 tctgcatatt tttgaagata tcggcgatat aggaaatgag tatatcttta aacaaccttt
    28201 ctgtgatcag ccaatcctat ccagtaacaa agaaaactct gaagtaaaag tattagtaga
    28261 tacaccggaa atagcaaaaa taagtattct acaggaaatg gaaatccctg tttctgcaga
    28321 cgagcgttta gaaaaagaac agcagatggt tgtggaattt cgttatcgaa aagcagaacg
    28381 atccaaagaa aaacgaatcc ttcaaataaa aacaatcatg acaatcagaa aagatagtaa
    28441 aaaaatcgac tttgaaacga ctctcgacaa tcagatgaaa gatcatcgct tacgtgtctt
    28501 gtttcctaca aagttacatg tggaacatca cgaagcagat agtatttttg aagtggtgaa
    28561 gcgcccaaac catgtgtcta aaagttggga aaaccccacg aatccgcaac atcagcaagc
    28621 atttgtcaat attcatgatg aagagtacgg tgtaacagtc ggaaactttg gtttgaatga
    28681 atatgaagta acagaagacg gacaaatcgc agtgacgcta cttcgcagtg tgggagaact
    28741 aggcgattgg ggatattttc caacaccaga agcacagtgt ctaggtgagc atcggttcaa
    28801 ttacagcatt gaactacatg gaccagaaga aaaattctct acttatttgc atgcatacgc
    28861 agcacagatt cctttttcca ctcaacaaat caaacatcat gaaggaacat tgatttcaaa
    28921 acaacaatat ttaacgataa aaagtgaaac gtttgcaatt acagctttga aacgcagcaa
    28981 gtttagtgat aaagtagtag taagaggttt caatatgtct agtcatttag aaaaattgga
    29041 aataactaaa gacaatggaa aaactgtcat attgaatctg cttgaagaac caacgaagca
    29101 agcggttgtc ccaataatcc aaccttatga gattcggaca atcggattcg aggaggaaaa
    29161 cgaatgtatg gcggaataga agcaggcgga acaaaatttg tgtgtgcggt ttcagatggt
    29221 ggagaaattt tagaaaaatt aagtatccct accacaacac cagaagaaac attggatcag
    29281 gtatttgaat tttttgatcg ttatgaattg aagggaatcg ggattggctc attcggcccg
    29341 attggtatcg acaaaacaaa agataattac ggatacgttt tggcaactcc caaaaaaggg
    29401 tgggcacaat tcgattttct tggcacaatc aaggaacgct acaatattcc tgctgcttgg
    29461 acaacggatg tcaatgctgc tgcatatggg gaattgaagc aaggagcagc acaaggaaaa
    29521 aatagttgta tttaccttac cgtgggaaca ggaatcggtg caggtgtcgt gatcaatgaa
    29581 gaaatttttt cagggatcgc acatcctgaa atgggacata tttgggtgaa acgccatcca
    29641 gatgaccatt atgaaggtac ttgtccttat cataaagatt gtttagaagg attagcagca
    29701 ggaccgtcta ttgaggcacg cacagggatc aaagggcaag atctgccgca agatcatcct
    29761 gtttgggcta ttcaggctta ttatattgct caagcactga tcaactatac gttgacgtta
    29821 gcacctgaaa agattatttt aggcggtggg gtaatgaatc aggatcatct ccttcaaaaa
    29881 atccgtcagc aatttgttga attgatgggt ggttatatgg aaacacctca agtaagtgaa
    29941 tatatcgttc gttggggact gccgaatgaa agtgggatta tcggtagttt gctgttagca
    30001 gaacacgaac atcaaaagta agcagacaaa ttacacaaaa agaacagagt cttatcatta
    30061 tctgctgata agactctgtt cttttttatt tctattccca taatccaggt aaagctttac
    30121 cgactatttt ttcttgagta aatggatgga tcatgtgcag ttctaatgca tggagcatca
    30181 gtcgttcagc tgttctattt tggtagagag ggtctccaac gatcggatgt cccttatgag
    30241 ctaaatgaac tctgatctgg tgtgtacgtc cggtttccaa aacacaatag acttgtgttt
    30301 gttcagaaag tactttgttg acagtgacat gtgtcaaggc gctctgacca tttttatcat
    30361 ctacgaccct ttttctccga tcatggcgat ctcgtccaat tttattggtg atggtcatat
    30421 ctttggcaat ttctccccat acgatggctt gatatctccg atagatcatt ttttgttcca
    30481 acattcttcc aagtatcggc aagacaaatg gattttttgc aaataaaacg gcaccgcttg
    30541 tttctttgtc caagcggtgg acaacgtaag gtcgttgctg tttagtgtcg agataagcag
    30601 ctaaatgatt gagtaaggta ctgttttctg tcggttcatt tggatgagtc ttcattcctg
    30661 caggcttatc cactataatc aaatgctcat cttcatataa aacacggatg tgcttcgcat
    30721 ctcctaattg aacttcttga taattataat ctgtttcttc gaataacaat gtgatctggt
    30781 cattgccctt tacttcatga tggaacaaag caggttgttg attgatccaa acgtttttac
    30841 gtatacgcaa aaagtgtcga acctttctag gaactaacca ttccttttca agcaagtcgc
    30901 gaattgttgt ttcaggatgg ttcataggta aagtgatcgt gattttcaca agtgtactcc
    30961 tttcagtgca attgcagact aattgtacca tttttaaaac atatttaaga aaaaacttac
    31021 atttttctgt tcattcttat gttactctac ttgcactaac gtaaaaaaac atattgaact
    31081 acaacttaag caggaagaaa acactctttc actatggtaa aataagacga ttgaatcaaa
    31141 tagacaggag aagaagcaaa tggattttca aagcatttta gaaaagataa aagctgcttt
    31201 gatcagtttt tggcgttgga tcagaccata tctcggacaa ttccatcgat ggcgaaagcg
    31261 catctggaaa aaatatcatg tgaacaaatt gattttgctg attggtttga tctgtgtctt
    31321 agtgacaagt atctacctct ttattttagc aaaacaagca aatgtcgaaa cgttaaaatc
    31381 aggattaagc caatcgacag tcatttatga taagaaaaac gaagaagctg gcacactgta
    31441 cgcccaaaaa ggaacatatg tagaattaaa cgcgatttct ccctatatcc aagatgcagt
    31501 aatctcgaca gaagatagga atttttacga gcatcacggg tttgatatca aagggattgc
    31561 ccgtgcagca gtccgaatgg tcttgtctgg cggaacatca gggggcggag gaagtacgat
    31621 cactcagcag cttgccaaaa atgcgtatct gacattagac caaacgtttg atcgaaaagc
    31681 gaaggaatta tttttggcaa tcgagatcga aaagaagtac agcaaagaag aaatcttgac
    31741 catgtatctt aacaatgcct attttggcaa cggggtatgg ggtgtccaag acgcttccag
    31801 aaagtatttt ggagtagatg caacgaatgt tacgctgtct gaggctgcta ctttggcagg
    31861 aatgctgaaa ggtcctggta tttataatcc gatcgatcac ccaaaaaatg cgaatgatcg
    31921 gaaaaacaca gtactaagtg taatggaaga aaacggaaaa atcacaaaag aacaagaaca
    31981 agcagaaagc cagacagata tcagcgcgta tcttaatgac acatacgata attcagattc
    32041 tggttatcgt tatccttatt attttgatgc agtgatcgat gaagcagtta acgagtatgg
    32101 tcttgatgaa gaagatgtca tgaataaagg ctataaaatc tatacagctt tagatcagga
    32161 ttatcaaaag caattagaag ctacttatca aaacagtgca ttattcccag caaatgcttc
    32221 agatggtgcc atggtccaat ctgcttcagt agctttagat ccgaatacag gtggtgtaca
    32281 agcactagta ggacgaagag gagatcatgt ctttagagga ttcagctttg ctacacagat
    32341 gcgtcgttca ccaggatcaa cgatcaaacc attaagcgta tatacaccag cactggaagc
    32401 tggatacaag ccttccagta tcttgaaaga cgaaccgcaa agttattacg atgcgcataa
    32461 ttttgacggg acataccaag gagaagtacc gatgtatcaa gctgttgcac aaagtttgaa
    32521 tttgcctgct gtatggcttt tgcatgagat cggtttacaa aaaggatatg ataaagcaga
    32581 agaattcggc ctgcctttag caaaatctga taagtattat ggcttagccc ttggtggact
    32641 ggaaaaagga gtatctccat tgatcatggc gggtgcttac agtgcatttg ccaatgatgg
    32701 tcagatgtat acgccgcatc ttatcacgaa aatcgttgat tcgactggag cggtgatcgt
    32761 cgataacaca aacaaaaaac caaaacaggt catatcaaag gaaacagccg atgaaatgac
    32821 cagcatgctt cttggtacat tttcaaatgg aacagcggct gctgcgaatc ctgcaggtta
    32881 tacgatagca gggaaaacag gaaccacaga aactaatttc gatgccacaa aagcaaatga
    32941 ccaatggatg atcggttaca cgccagacgt agtgatttcg acatggatgg gttacgaaga
    33001 atccagcaag ctgcactatt tggaagggac aagtggaaat gttgtcggga aagtcttcaa
    33061 atcagcggca gaaggcatcc ttccgtatac ttcaaaaaca ggatttagtg tagcagatgc
    33121 ctatgccaca ggaggacaag tggtagctgc agatcaagtt ggaaacacag gaaattctaa
    33181 tggagagtct ggaaattggc aagataactt gaatcaatat ggtgaacaag caaaagaagg
    33241 gctgaaaaac ttcggcgata tggtgaaaga aggcgttcag ggaattggtg aagcggctaa
    33301 agacctctgg cggaaatatc aaggagagta gcccctttga aaacgaataa ccccatgtta
    33361 caatagagag taactactaa aagaaaagag ggcataacat gtcaaacatc tacgatagcg
    33421 cgaatcaaat cgaacgtgag atccgtgaat taccagaatt caaagcgttg aaagaagcgt
    33481 acgagaaagt acaagcaaac gaagaagcac acaaactgtt caaagacttc caagcgatgc
    33541 aaatcgaatt acaagaaaaa caaatgagtg gacaagagtt cagcgacgaa gatgcaatga
    33601 aagcacaaga aatggctgtg aaaatccaat cagaagaagt aatcaatgac ttgatgcaaa
    33661 aagagcaagg cttcagtaca atcatcaatg acttgaaccg tatcatcatg acaccagttc
    33721 gcgaccttta cagcgaataa aactatttta tgaaacaaca gagggtgtga aagagacgtt
    33781 cggccttgag cgatgacaaa attgcttctt attgccgata ggctgtctct tgaacaccat
    33841 ttaccaagag gtcgtgagcc taccgtttaa ctctgagtta caaggaacaa tttcaaaaaa
    33901 cagcttctca tgtttgatga ctattgtgac ttgtgaccga ggagttggca ggagaacacc
    33961 gtttattcag aaataggagc tacgacaaaa gtcttgtcat agctcctatt ttttgtcatt
    34021 catttttcaa gcggtagaaa tagccttttt gagctaacag tttttcgtaa gtacctattt
    34081 caacgatttt tccttcatcg atcacaacga ttttatctgc ctctttgatc gttgaaagcc
    34141 gatgagccac aataaaagta gtgggtgttt ctgtcaggta attcagggct tgctggattt
    34201 ttttctctga ctggttatct aaagctgagg ttgcttcatc tagcaaaatg atttttggct
    34261 gacgtatcaa cgctcgtgca atggagatac attgcttttg gccaccagac agcttgttgc
    34321 catgttcgcc tactaaggta tccagaccat caggaagctg gtcaatcagt gaagaaagtt
    34381 gcgcagcttc gatgacttca tctaaacgtt cttttgatac gtttttcaag ccataagtga
    34441 tgttctcttt gatcgaagcg gagaacaaca aagtggtttg aggaacaacg gctaaataat
    34501 ttcgcacact tcgcatatcc agttcatcca aaggctgatc atctagaaaa atttttcctg
    34561 aagtcggctg gataaatcca atcaataagt taaggagtgt agattttcca gagccagatg
    34621 gcccaacaaa agcaattgtt tctccagctt tcacctctag agaaaaatga tcgataatcg
    34681 gtttgagtga gtccggataa agaaaagaaa catcctcaaa acgaaaattt ccatccaaat
    34741 gttggatttt tttcttaccg tgataagttt ctacatcgcc agcaatcaac agttcgccaa
    34801 tagaagtgat agattccatg ccttttgaaa tcgtaggaat cagcgtgatc attccagaaa
    34861 cggaattgac gatcgtcgtg aaatacgtct gatacatcac gacatcgccg ggttggatgc
    34921 gcccttgtaa agctaatatc ccagtgaaac tcaaacaaat cagctgaaat atctgaaaac
    34981 tagcccagct gactgatccg aaaacagatt gcaggatatc taagtggaat cctttttcat
    35041 agatttgagt aaaatgctta tttaagcgag ttgtttcgtg tttttctaac ccatgagctc
    35101 gagcaatcgg tgctaattcg atcacttcga tcacttttgc agaagtctct tccatttctt
    35161 gcctgaagtc tgagttggac tgtctgatcc ttttacggaa aaagagagta acgaatgcgg
    35221 cgagaggaac agtcaataag aaaaacatga aaacaacagg acttttataa agcgtgatac
    35281 ctaaagctac gaataaattg acagcgatat ttaatccgct gacaaatact tgagaggaca
    35341 acgtttggat agcttctaca tcacggatga tctttgactg gattcgtcca gattgcaatt
    35401 ctttttgata aggaatagat agttcttgga ttttcttgat aagggtcgct cttagtccag
    35461 cttctacttg acggatcgaa gtgctatggt attttacatg aaaataattc gtcaatatat
    35521 tttgaccaat cagacaagcc atcaaaaaag cgttgaaata aatggcctgc aactgagaag
    35581 aatctttcga tgaagctgca ttgattacat tggcaatagc gatcggcatt aaccaagatg
    35641 gaagatgttt gataataaaa aagagtcctg acatggcaaa acgccaataa tttccctgat
    35701 aaagatgcag caatgtctga aaagcatttg catgatattt ttgataactt ttctgtattc
    35761 gttcctccat aatcttctcc ttaacaatct tggtaaataa actgcaagtg ttctattgct
    35821 tcaggaacat aaatctcagg agtagcttca agagtggcgt acattagagg acgctcatat
    35881 ttgatataat gcagtaccgg gcggaaatct aaacagccct tgccaacagg taccatgcgg
    35941 atctttccat tttccatgat atagtctttg agatgaagcg ctgtaatata accatgtaaa
    36001 ttgtccaagg cacctcggat cactttttcc tgatcagcat gattttcttt atgcatcaaa
    36061 ttcgcacaat ctaaaatgat ttttaaattt ggtgaagcta cttcatcgat taaacgttta
    36121 gctaactgat agttatagat tggatgattg atccctgctt caacagcaac agttacgcca
    36181 aaattttctg catgagcgac taattcaatg atagaatttc tggcgatctg ataagcttct
    36241 tcagtaaaat tttcttttgt gtaaccgaca gagacactac cggtttccgt agcaatcatc
    36301 ttggcttgat aggcagagca cagagcaatt tgctgtttga aagttgccaa tgcttgtgca
    36361 cgaacttctt gatttttgct ggataggttg atatagcatc ctaaaatgga aagttcgata
    36421 ttgttttttt ggaagtattc accaaaatag gcagcactcc caagtgtcat gtcagttaat
    36481 ttaagttgtt caggaaatgc tttttgtggt gcaaattgga tatgggacaa gtgaagctgt
    36541 gatacttttt gaacgatttc ttcacgattt cgaacatcta aatcgtgggc acggatgcct
    36601 aaatttaatg gaaacatctc aaactccttt ttgtaaataa ttagcaagag gcagacggga
    36661 ttcttttatc aattcaacaa ttattcgggc aactttatca gcgccttctg gactaaagtg
    36721 ggtattgtct tttagtcctt ctgggtaatt tttacaagta tcaggcataa gatgcaggta
    36781 gaattttttt gtctcttctt ttgagaaggt ctggaacaat tcttgtgatc ttgagaacac
    36841 atctaaaaga ggaatatggt gtttctcagc aaatgcacgc atcgcttttg gatagtctcc
    36901 aagtatatct ggattcaatg ttccgttttc taagtaatct cgtcgcgtca cagatgtcag
    36961 taagaccatt tggacgttcg ctttttttgc gcattcaaaa aatatctgca gattttttaa
    37021 atagctaccg aagggaagcg tacctctttc ttcttggatt ttctggtcgt tatgtccaaa
    37081 ttgtacaaat aagaaatcat tcggctgaaa ctgtgaagct agacgggcta accttccttc
    37141 ctggataaat gatttggtgc tgcgtccatt ctttgcttgg ttattgatct ggatctcggg
    37201 tgaaaaataa gcagagattt tttctcccca acctgtctcg ggacgcttgt tttcatcttt
    37261 ttctgccgca gtagaatcac cggcaatatg gatagtaggc atgttatctc ttctttcata
    37321 aaggtttata ggcaatgaag cagaacagca agagaagaag acggagca
//
LOCUS       DAZUKC010000022        34789 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000022 DAZUKC010000000
VERSION     DAZUKC010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.023"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(233..1081)
                     /locus_tag="ACG80T_001021"
     CDS             complement(233..1081)
                     /locus_tag="ACG80T_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4840688.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1305..3245)
                     /gene="thrS"
                     /locus_tag="ACG80T_001022"
     CDS             complement(1305..3245)
                     /gene="thrS"
                     /locus_tag="ACG80T_001022"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFS4840689.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3582..3761)
                     /locus_tag="ACG80T_001023"
     CDS             complement(3582..3761)
                     /locus_tag="ACG80T_001023"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFS4840690.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3808..5631)
                     /locus_tag="ACG80T_001024"
     CDS             complement(3808..5631)
                     /locus_tag="ACG80T_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS4840691.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5909..8188
                     /gene="helD"
                     /locus_tag="ACG80T_001025"
     CDS             5909..8188
                     /gene="helD"
                     /locus_tag="ACG80T_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFS4840692.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8281..8928)
                     /locus_tag="ACG80T_001026"
     CDS             complement(8281..8928)
                     /locus_tag="ACG80T_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840693.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(9107..9826)
                     /locus_tag="ACG80T_001027"
     CDS             complement(9107..9826)
                     /locus_tag="ACG80T_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4840694.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9957..11108)
                     /locus_tag="ACG80T_001028"
     CDS             complement(9957..11108)
                     /locus_tag="ACG80T_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFS4840695.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11325..12065)
                     /locus_tag="ACG80T_001029"
     CDS             complement(11325..12065)
                     /locus_tag="ACG80T_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4840696.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(12069..12392)
                     /gene="rsfS"
                     /locus_tag="ACG80T_001030"
     CDS             complement(12069..12392)
                     /gene="rsfS"
                     /locus_tag="ACG80T_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFS4840697.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12449..13042)
                     /gene="yqeK"
                     /locus_tag="ACG80T_001031"
     CDS             complement(12449..13042)
                     /gene="yqeK"
                     /locus_tag="ACG80T_001031"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFS4840698.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(13026..13676)
                     /locus_tag="ACG80T_001032"
     CDS             complement(13026..13676)
                     /locus_tag="ACG80T_001032"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFS4840699.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13686..13997)
                     /gene="yhbY"
                     /locus_tag="ACG80T_001033"
     CDS             complement(13686..13997)
                     /gene="yhbY"
                     /locus_tag="ACG80T_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFS4840700.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(14013..15122)
                     /gene="yqeH"
                     /locus_tag="ACG80T_001034"
     CDS             complement(14013..15122)
                     /gene="yqeH"
                     /locus_tag="ACG80T_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFS4840701.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(15119..15646)
                     /locus_tag="ACG80T_001035"
     CDS             complement(15119..15646)
                     /locus_tag="ACG80T_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFS4840702.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15826..16611)
                     /locus_tag="ACG80T_001036"
     CDS             complement(15826..16611)
                     /locus_tag="ACG80T_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFS4840703.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16604..17473)
                     /gene="accD"
                     /locus_tag="ACG80T_001037"
     CDS             complement(16604..17473)
                     /gene="accD"
                     /locus_tag="ACG80T_001037"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFS4840704.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17474..18853)
                     /locus_tag="ACG80T_001038"
     CDS             complement(17474..18853)
                     /locus_tag="ACG80T_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFS4840705.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18861..19280)
                     /gene="fabZ"
                     /locus_tag="ACG80T_001039"
     CDS             complement(18861..19280)
                     /gene="fabZ"
                     /locus_tag="ACG80T_001039"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFS4840706.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19305..19781)
                     /gene="accB"
                     /locus_tag="ACG80T_001040"
     CDS             complement(19305..19781)
                     /gene="accB"
                     /locus_tag="ACG80T_001040"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFS4840707.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19784..21019)
                     /gene="fabF"
                     /locus_tag="ACG80T_001041"
     CDS             complement(19784..21019)
                     /gene="fabF"
                     /locus_tag="ACG80T_001041"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFS4840708.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(21038..21775)
                     /gene="fabG"
                     /locus_tag="ACG80T_001042"
     CDS             complement(21038..21775)
                     /gene="fabG"
                     /locus_tag="ACG80T_001042"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFS4840709.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21787..22704)
                     /gene="fabD"
                     /locus_tag="ACG80T_001043"
     CDS             complement(21787..22704)
                     /gene="fabD"
                     /locus_tag="ACG80T_001043"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFS4840710.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22811..23035)
                     /locus_tag="ACG80T_001044"
     CDS             complement(22811..23035)
                     /locus_tag="ACG80T_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFS4840711.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(23088..24050)
                     /locus_tag="ACG80T_001045"
     CDS             complement(23088..24050)
                     /locus_tag="ACG80T_001045"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFS4840712.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(24060..24500)
                     /locus_tag="ACG80T_001046"
     CDS             complement(24060..24500)
                     /locus_tag="ACG80T_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS4840713.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24806..25771
                     /locus_tag="ACG80T_001047"
     CDS             24806..25771
                     /locus_tag="ACG80T_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFS4840714.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25828..26058)
                     /locus_tag="ACG80T_001048"
     CDS             complement(25828..26058)
                     /locus_tag="ACG80T_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFS4840715.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(26144..27031)
                     /locus_tag="ACG80T_001049"
     CDS             complement(26144..27031)
                     /locus_tag="ACG80T_001049"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS4840716.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(27120..28343)
                     /locus_tag="ACG80T_001050"
     CDS             complement(27120..28343)
                     /locus_tag="ACG80T_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS4840717.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28360..29406)
                     /locus_tag="ACG80T_001051"
     CDS             complement(28360..29406)
                     /locus_tag="ACG80T_001051"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS4840718.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(29570..31297)
                     /gene="ptsP"
                     /locus_tag="ACG80T_001052"
     CDS             complement(29570..31297)
                     /gene="ptsP"
                     /locus_tag="ACG80T_001052"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFS4840719.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRGLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(31297..31563)
                     /locus_tag="ACG80T_001053"
     CDS             complement(31297..31563)
                     /locus_tag="ACG80T_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFS4840720.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            32042..34276
                     /locus_tag="ACG80T_001054"
     CDS             32042..34276
                     /locus_tag="ACG80T_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4840721.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34424..34744
                     /locus_tag="ACG80T_001055"
     CDS             34424..34744
                     /locus_tag="ACG80T_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFS4840722.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPQH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT         9843 a         7511 c         6063 g        11372 t
ORIGIN      
        1 aagaaaaaat gggcggagca agaaattctt tacctaattt caagataaaa tcaacttttt
       61 gtttcacagc aatcacttaa agttacatat ccaactattt ttttcattaa aaaataaact
      121 tatttttgaa aattatgttt tcttaagtcg tgaaacactt tgatggatca tttatttgtg
      181 gaaaactacg ttttgttatt ctaaaaaact tatataaaaa atagtaaggt ttttatttcg
      241 aatccataaa atattcttgg attgcttctc gtgttttttt cactttttcg ctaagctttt
      301 cgtttttagc catatagaaa taaaaaatga tatcgatcgt agcaaattgg gccaaaagtg
      361 aattagttgc cgcacttcga ttcgtgattt cttttggtct agatgtttgt acgagtacat
      421 cggctatctt gctaagcggg ttatttccaa attgcgtcaa cgcaatgatt tcgatattca
      481 tgcttttcgc cagctcggct aatgcgacta catctgcact tctacctgag tttgaaacaa
      541 gccaaagcac tttctttttc tcattgctga caagttgcgg gagcaagacg tggatatctt
      601 tttcaaaaat gattggctta ccgacacgag accatttttg cagaatatct tctactacta
      661 aagaagaggc gccaacccca tataaaaaaa cagtctctgc cgattccaaa cactctacga
      721 ctttttggat cgtttcttct tctagaatat ctactgtttc ttttatcgct acttgtgcat
      781 tgttaccaag tttgtttttg atagagtaaa aggactcatt tgcttctata tcactaaaac
      841 cttcatcgat tccagtctgt agttctgttg aaatcgtgat tttgaagtcc gtcagacttt
      901 gaaaaccgat ttttttggaa aaacgtacaa ctgtcggcgc actcaacccg gaagcttctg
      961 ctaattcatt agctgtcatt gtagggacct tctcaatatt accgataatg tagttggcca
     1021 actttttttc tgctgaagaa taatcttcca tcatactttc aatacgacta attacgctca
     1081 taagttcctc caccctttta atgatagtct atgtaagtat accatatcag acccttattc
     1141 acggtcgtat actggataaa aaacaaaaaa acaatccgcc caacagaata tcttatccca
     1201 tcaatcccac agattctatt gagcatctgt gtctttgagg aatacctgtt atctattgtt
     1261 cggattgctt tctatctact acttttttac aggaaattta cctattagtt ttctctgctg
     1321 taatttttga cttctgcttc catagcatca ataaagatat tcaaatcttc tacagctgtt
     1381 tctttacttc cataacgacg tacgttcact gttgcatctt ccaattcctt gtctccaaca
     1441 actaattggt aagggatctt ttgtgtttga gaggcacgta tcttatagcc cattttttca
     1501 ttacgatcat caacttctac acgcaacccc ttcatttgca aacgttcttt gatttcataa
     1561 gcataatcgc catgtgcatc aacagaaaca gggatgattg ttgcttgtat aggtgccaac
     1621 catgttggaa aagcgccttt gtatacttct gttaaataag cgacaaaacg ttccattgta
     1681 gaaacaattc cgcggtggat aactactgga cgatgcgtat tttccccgtc ttctccaaca
     1741 tatgtcaaat caaagcgctc tggcaataag aagtctaatt gtatagttga taatgtttct
     1801 tctgtaccaa gagctgtttt gacttgaaca tctagttttg gtccgtaaaa ggctgcctcg
     1861 ccttctgctt caaagtaatc tagttctaat tcatccatag cggctttcag cattgcttgg
     1921 gctttttccc acattgcgtc atcatcaaaa tatttatctg tattgtttgg atcgcgatag
     1981 ctcaagcgga aacgataatc tgtaatattg aagtcagcat atactgccac catcaattca
     2041 agcgtacgtt taaattcatc tttgatttga tcaggacgaa caaatgtatg accatcattc
     2101 aacgtcattt ctcgtacacg ttgtaaacct gacaaagcac ctgatttttc atagcgatgc
     2161 atcattccta attcagcgat acgaattggt aattcacgat aagaatggat cgtgtttttg
     2221 tagaccatca tgtgatgagg gcagttcatt gggcgaagaa caagcatttc gccatctccc
     2281 atatccattg gcgggaacat gtcttcttga taatgatccc agtgtccaga tgttttgtac
     2341 aactcaacat ctcccattat tggtgtatat acatgttggt aacctagact aacttctttg
     2401 tctacgatat aacgttcgat cgtgcgtcgg attgttgccc cttttggaag ccagaatggc
     2461 aaaccagaac ctacttctgg ggaaaccatg aacaagtcaa gttcttttcc tagtttacga
     2521 tggtctcttt cttttgcttc ttcacggaga cggatatatt ctttcaaagc tttcttgtca
     2581 aaaaaggctg tcccgtatac acgttgcatc atttgattgt ttgagtttcc acgccaataa
     2641 gcacctgcca ctgataataa tttaaatact tgtatacggc cagtggatgg aacatgtggt
     2701 ccacgacaca aatcgatgaa ttcgccttgt tgataagcag taatcacttc ttcttctggc
     2761 aattcactaa tcagttctac tttgtacgga tcacttgcaa aaatttctaa tgcttcctgt
     2821 tttgaaagaa cacggcgttc gatcggcaga ttttctttga cgattttcat catttctgct
     2881 tcgattgccg gcaaatcttc tgcagttact tggttttgtc cattatctgt atcgtagtaa
     2941 aaccctgaat cgatagctgg tccaacgcca aagtggatat ttggatagag gcgacgcatg
     3001 gcttgggcca tcaaatgagc tgctgaatga cggactaagc caagtgcatc ttcatggtct
     3061 ggtgtgatga tctcaatact tgcatcttcc tcgattggac gagtcaaatc aacgagtttt
     3121 ccgttcactt taccagctaa agcttttttt gctaagcttt ttgaaatgct ttcagcaatt
     3181 gccaatgttg ttgttccaga ctcaaattct ttgactgcac catctggaaa agtaattttt
     3241 atcataatag tttcctcctt gaaatgtttt gattgatcag atcatcaagc tgaaaaacaa
     3301 aaaaatccta gcatctcttg aaaagagata ctaggacgaa aagttcgtgg ttccacctaa
     3361 tgttcaaacg caaatgcgtt cctcaagcgt tgtaacggtc cgaccgccct tgcttcaaca
     3421 agggtttcga aagtggtcat cctttggttt tctcctggaa aagtttcagc ctagcttttc
     3481 ctctcttatc gaagactctc aaattcagtt ggctttctca tcaatcaatt atgtgtttat
     3541 ttaaccacta ataaatagaa aaatcaagtg ggaaactaaa cttatttttt ccattcccca
     3601 tttacaccgt atgttccttt tttcatctca ttgatgatga catggatgtg ttcttttggt
     3661 gcctgcgtgt ttttatgaac agcttccgtg atatctttca tcatattttc taattgttcc
     3721 ggactgcgac cttctactaa ttcgacatgt acgaatggca taatgctctc tcctctttct
     3781 gatcgttctt ttttcaactc gattctatca tactatgttt gtcttgggta ctgaatcagg
     3841 aaaaatggct ggatcttttt ttgctctcaa ggcactcaaa atagagaccg tatccacgac
     3901 ttcttgcaaa acagctccga ataatgcggg gattagtccc gtacttgcaa ttaacatcaa
     3961 aaaaatacaa ataaagatgc caatcagtac agactgtttc gctattctca tcgtatctct
     4021 tgaaatcact actgcttttg ctacacgcaa gagatcatct ttcaagatga ccgcatctgc
     4081 actttcacta gcagctgtag aaccatgagc ccccattgca ataccgatat ctgcagcagc
     4141 aagtgctggt gcgtcattca cgccgtctcc taccatcgca acaggtcttt gatctttagg
     4201 gatactttct aaaatggtca gtttatcttg aggcaagcta cgggcatgta cagaatctaa
     4261 ttggactttt tctgcaatcg tttgagcaat ggcagatcca tccccggtga tcatcatcaa
     4321 atttttcaca tcaagttttc ttagctcctt gattgtcttt gctgcttctg ggcgaatctg
     4381 atccgaaaat aggatcgttc ctgcaaaaga tcgatcaatt gttacataga ttgctgtatt
     4441 ggacgtttct atttcttcag ctgaaacaaa agaggcacgt ccaatcttta cttcatgatt
     4501 attgactact gcttggattc cttgaccggt cacttctttc agttgactaa caggatacag
     4561 ttggctgttt ttcacgttgt tcactaacga acgagctaag atatggctag attcctgttc
     4621 tgcactagct gcatacagaa gtagctcttc tttttttatt ccattgactg gctgaatcgc
     4681 ctctacttcc aatatccctt tggtaagcgt tcctgtttta tcaaaagcaa tcgtttttgt
     4741 acgtgcgagc ttttcaatcg ttgttccagt ttttacaacg atcccgtttt tacttgaacg
     4801 gctcattcct gcaactaaag caatgggcgc agctagaatc agtggacaag gtgatgccac
     4861 taccagtacc tcagcaaatc gtaaagggtc ttttgagaac caccaagaaa taccaccgat
     4921 caaataagca atcaacgtga aagggactgc ataacgatca gctaaacgga caaatcgggc
     4981 aggcttttct tttgattctt cgactagttt aataatcatt tgatactggc tgtctgcggc
     5041 acgtttttca gcgatcattt ggatcgatcc gtctccattt aaagaccctg acataatctg
     5101 atctccaggt ttcttggttt ccgggcggga ctcacctgtc aaagaagatt catcgaccga
     5161 agattctcct ttggtcactc gaccatctgc gggtacaagt tcgccaggct tgactagtaa
     5221 ctggtcatca atatttattt catcgattac tatatcctgc aattttcctt ctttgaatcg
     5281 atgggcaatt cttggggtgt tatctaacaa agactgcagt tctttacccg ctcgtttatt
     5341 cgcgtaatcc tctagactat ctccgcccgt aagcatgatc aatatcatca aactagccca
     5401 gtattctttg acggctaatg ttgcaaggat tgcggtaatc gctaatacat ctacgccata
     5461 tcttcctgaa cgtaacgtct tgatcatttc caaaaacatc aagaaggcga gaatccctcc
     5521 agtaaatgcg acaatcgcaa atgcaagggc cggctggtga aaaacaaatt cacttaataa
     5581 agctatccca ccaataagca aagtaagtaa aaattttttg aaatgtgtca ttgttttttc
     5641 tcctctttta tgtgacttag agaggttagt aataaatttc cagctgcctc tttcatctta
     5701 agcatagtcc ttgactagca ctttttaaaa caataggcct taattgaaaa caactatcac
     5761 tatcagcgtt tccaaaattc atcagttcta caaacaaaaa tattcagcaa ggattacttt
     5821 ttcgattcat cagttaaaaa gtaccttttt tcgaaaaact atgctatact tttaattcgt
     5881 tatttacggc ggaaggagca aacatgcgat gaatgagaaa caactagaac aaacgcacgt
     5941 ggatgaaacg atcgaactaa tccagatcga acaaaatatg ctggatacaa aacaacaaga
     6001 attgactcag gaaatgaatc attcgacaaa agacacagct aatcacaaga ttcgagctgg
     6061 ttccaatgaa gctttttatg aatctgctgt tgaatatcgc caacatgagc aggaattgct
     6121 tttgaaatac catactcttg aagcacagca aaaacgcctg caaacgttgt ctgtcatgaa
     6181 agatagtcct tattttgccc gtatcgactt taaagaagac cgtgaaacag aaacattata
     6241 tctaggtatc gcttctttaa gagatctggc agaagaaaca atcgttattg actggcgcgc
     6301 accaattgcc aatctttatt atgaaggtga gattggtccc gcattttatg aaactgaaac
     6361 agatagaata gatgtcgatc tgcagttgaa acgacaattt aaaatccaag aaggacgtat
     6421 tttatctatg gtagatacgt cggaagtaat caatgatgac tttttgctag agatattgga
     6481 tgaatcatca agcaaccata tgaaaaacat cgtctctacg atacaaaaag cacaaaatgc
     6541 aatcattcgg gacacaacaa gtaaagtaat gctgattgaa ggaatcgccg gaagtggaaa
     6601 gacatccgct ttgcttcaac gtattgcttt tctgttgtat cataatcgca agtggctcga
     6661 ggctgaaaac gttcttctat tttcacccaa ccatttgttt tctgattaca tctcgactgt
     6721 attgccttca ttaggtgaaa gcggcgtgcc tacacagaca ttcaaaaatt atttggtgca
     6781 gctgctgcca gagttttctt tactagatga acagcaacaa gaaagtggtt ttttgctagg
     6841 ggaaaaagat ccgatacaag tgatgaaaag cggattgacc ttagtcgatc agattgatcg
     6901 ctatatccaa ggcatcacaa actacggtcc cttgttccgt gatatgaaaa taaatggccg
     6961 tacgatccta tcaaaggaat cgattcgcca atggtataaa gaaacaaatg aacaattacc
     7021 tttacatcat agactttctc tattacaaac gaagctcctg aaaaaattag gcggtctgca
     7081 aaaagacgaa acacgtcagc aatgggtcaa agatctggcg gaagaacagc tccaagaact
     7141 atacgcaaca gatccaaatc ttgaatatac agaacaaaag gagcgttcat tacgtaaaaa
     7201 actggccaac caaatcgtac gtaaaagatt ccgtcgaatc catcgaggga tcatgaatta
     7261 tcaatttgtc aatataacga aacaatatct ccactttcta caaactgtgc cactttctct
     7321 tctaacacga cataaaatca gcgagaccag gtggcaggaa catttgacaa ctgttcgtac
     7381 acacttgcgt caaagagaat tgcgtcaaga ggatgctgta ctattctttt tactgatgcg
     7441 ccgaacatat ccagtgacgg ttacacacaa agctcgctat atctttattg atgaaatgca
     7501 ggatttcgca ccatcacaag tggcattgct gcaagccttg ttcccaaaag caaatttgac
     7561 tttctgtgga gatctaaatc aaaatgtctt tggaaatgaa acgatcgttg gatcattgga
     7621 agaccttttc gtggaccgtg aagtcactcg tttccaatta acgacaagct atcgctccac
     7681 aaaagaaatc actgattttg ctaatcattt tattgtgaaa gaacatcaag tagaaacaac
     7741 agcaagaaaa ggagaccttc ctttgctcat ccaaggaaca acagatgata tcaaattgaa
     7801 ttggttagca gataccattc gaacgactca gggaaaagcc cggtattggc gtacagcaat
     7861 catagctaaa acacaagaag aatgcgaagc tctttatgct caactgccag acgagttgaa
     7921 acaacaagtc cagctgattt taaatgagag tgactttatg aagcgttcca ttattttact
     7981 tccagccttt ttagcaaaag gtctggagtt tgaccgagtt ttcttgtggg atgtagatgc
     8041 agaacatttt tcttctcagc aagacaaatt gatcctttac accatgtgta cacgtgcgat
     8101 gcatgaattg acccttttta cctccaataa tgaaagtcct ttgttaaaca atatggatat
     8161 ttcatcttat gagaaaaaag tactgtaagt aaaaagacaa ctgctatcat taatgttagc
     8221 agttgtcttt ttatttacaa taataattgc agaaaaagta attcaatcat ttaaggtcat
     8281 ttattctata tataaatcca aatctgtttc aggatatttt aatttgacaa gcgaaataat
     8341 cattttcagt tcttccatgt tttctggata ttctgggtca aataccttgc tatgacggcc
     8401 aatcccgcca ttgtcgatca atcggtaaat tcgattccat tcaatgaatt ggtcaattga
     8461 ttggaaattg ctgattggat agccgccaca atccaaaatc tctgatacac gaaatgctag
     8521 gtatgcacta tcgatcactt tatttttttt cgtgtctatc ttctcatttc tttcgataag
     8581 aattttttca ggacgatgtt tcaagctcca ctctaatagt agaaagaaag cctccatccg
     8641 atactcttct tcaaataacc gaaaggctga atcagaacga cgaaaaagca gcggaatcag
     8701 ataattttgt ctataaatca gcattggtgc gcggtccacc atacactcta aataacggtt
     8761 cagataccct gtaccgacat agtttcctaa tcgatagcct tcaacgccga atcttgcgta
     8821 tttttgcgct aattttgaac aatcagttac gttcacagct aactcttcta ctttgcggta
     8881 accgcttaag atacgttcaa aaggttctaa agtgattttt gcttccatat tcttccccgt
     8941 ccatcttcca catacttcat atcatttcac atgatactta tgctctcatc ataccacagg
     9001 ttgaattaga aaagaaaata aatcacacaa aaaagcaaga caatctgtta gtgagattgt
     9061 cttgcttttc gaaaaagact gaatactttt cgttaaaact ggttgcttag tcttctaatg
     9121 ccacattatg gaacacatgc tgtacatctt ctagatcttc cagtgcatca atcatttttt
     9181 caaatgtttc ttgatcttct ggagatagtg ttacctcgtt ttgagggatc atttggatct
     9241 cagcgattga aaaatcagtg attccttttt cttttaatgc ttcttgtata ctatggaaat
     9301 ccgctacttc accgtaaacg atgatttgtc cttcttcttc cattacatcg cgaacatcga
     9361 tgtctttttc cattaggtat tcaaggatct cgtcagcgtc ttctcctgca aaagcaaaga
     9421 ttgctgtatt gtcaaacatg taagaaaccg ctccggaaac acccatgttc ccgccatttt
     9481 tcccaaatgc tgcccgtacg tctgctgctg ttcgattcac attgttcgtc aatgtatcaa
     9541 caatcaccat tgagccgctt ggtccaaatc cttcatagcg caattcagag tatgtttcat
     9601 ctcctgcacc ttttgctttt tcgattgctc ggtcaataat atgttttggt acattataag
     9661 ttttagcgcg atcaatgacg aaacgtagtt tttggtttgc atgcggatca ggatcacctg
     9721 attttgctgc cgcgtagatt tcaatcccaa attttgcata aatccggcta ttattagcat
     9781 ctttagcagt tttctttgct acgatatttg cccatttacg tcccatattt tcacttcgct
     9841 ttctgatttg atagtttcat aacgcaaaaa cgtcctgttc cattatactt gtgaatcacg
     9901 attctgtgaa gctttttttc tttcttcctt cattcttctc atttttagtc aattatctat
     9961 actcggattg ggatttgccc gaaattctgt tccggaatat tgcctcccaa ttgatagatt
    10021 tgatcacttc taactaacag ttccgcttct tgtgcctctt tttttccgat tcgggaataa
    10081 atagaaatct cttttttttc ttttaagaat cttcttccag catcggtaaa tccaagtagt
    10141 cgaagataat ttttattttg ttggacagga atctcttgct ttttgatatt cagcaagaca
    10201 taacaagata atcgttggat ccgtgtccat gtatatcttt ttgtttttac ctttgacaca
    10261 aaatcctcaa aactatcagc agttgcagct gctgttttca tgcgcgattc tattccttcg
    10321 gtcatctgat agatatctgt cagttccagc aaagtagaag ataacaaccg ataacgcaaa
    10381 tacggccaat aagattccca agtctgcagc gaacaagttg aaagatcttc ttctgtctga
    10441 ggcggcacaa gtccagctat tctttttcct tcttctaacg catggcgaat ggctgttgca
    10501 ctggctatcc tctgatctgt gatgattttt tcatgatagc ctgcactttt acgtcggatc
    10561 ggatgtaatt ccatagggtg gacatacttg gcattttcct ttgcataact taatcccaag
    10621 atatgatttg gcgaagaaaa atcaagcgta atatcgggat acagactgcg aaaaacctca
    10681 gtcatttttt gcggataact atacgtttga tcagacagtt cttgaaatgc ctcttctatc
    10741 ctatcttgat tctcttggac aaagcgtccg aaactgctgt aatcaaacga agtctcttct
    10801 tctgttccaa aacacaaact gtcacagtcc agtgtttgca agatttttac tgcaccttta
    10861 gcaaaatagt cagctgactg aaccgcccat tcgacgggaa gctccactac taaatccaca
    10921 ccattttgta aagcttcatt cgcgcgatgc catttatcga tgatagccgg ttcgcctctt
    10981 tgcaaaaaat tcccgctcat tacagcaatg acgacctctg ctcctgttat ttttcgtgcc
    11041 atttccacat gatagcgatg tccattgtga aaaggattgt actctacgat aatcccgcag
    11101 cttttcatgc gcatcctcct tcatagttat cgataaaaaa gaaatagaga caagtaaaaa
    11161 aaggcaaaaa tcggaaaaaa tacccgaact atataaacta ttctcgcaaa cacaatactt
    11221 ctgcgacgaa aagctgcact gcaagagcaa tgcctatatc gttcggatat ataatttatg
    11281 ttgaagattt attcttttct tgttaactcg agcaggcaaa accgctagtc tttgtatgct
    11341 acaaagaacc agcgctttga tttctctgat gctgcttcgt ctgtaaaatc agaaaatgct
    11401 tcgactttcg aaaagccgct gttttccagc attcttaaat aactttccaa tggataggta
    11461 cgctcttgat gtagttcatc ttcacgaata aatcgaccag acccttcttg ttcttcaacg
    11521 aaaaaggtaa gaaaatgttc gatgctgtgt ttctcgcttc ctggataact atcccacaaa
    11581 aaagcaaaat tatccccttg ataatgatag ctatattctg gaaaaacctc atctacttga
    11641 taggtggagt gtacgtcaaa taaaaatcgg ccattttctt ctaacgcttg atagacttca
    11701 tcaaacactt gctgtacctc ttgtctattt ttcatatagc acagcgaatc agaaaaacag
    11761 gtaattgcct gatattgtcc gatttctgat aaatcgagca tattgccttc cacaaattgg
    11821 atcgatgcgt cttcttcata tgctcgctgg ctggcaatca tcaacatctc ttctgaaagg
    11881 tctaaccctg tcactttata tccatctttt gcaaaggcaa ccgctaatgc tccagtaccg
    11941 catgccagtt ccaaaatatt ttttgtgtct tcttgtaaat gacgtttcga aaaagctaac
    12001 cattgctgat aaagtgtttc gtccatcact tcatcgtaaa tgaaagcaaa tgtttcataa
    12061 gccatatgtt aatccaacca ttcactaaga tcgactagcg gagcatctga ccatagtttt
    12121 tccagattat agaaatttcg ttctggcgca tggaaaacat gtacgatcac atctccaaga
    12181 tcgatcaata cccattttcc gccttctttt ccttcaatac gttttacttc atatttgttt
    12241 tcttcttctt tatcaatgat ttcttctgta attgcgttga tttgacgttc gctatttgct
    12301 gaacagatca tgaaataatc tgccaacaag gaaacatttc gaacgtctaa agcgataatg
    12361 tcttctgccc gttttgaatc tgctgcttca atcgcaattt ttaattgttc tttactatct
    12421 atgatgattt cctcctaata tgagcagctt atttttttgc tacccagtaa ttatatgttt
    12481 cgattgtttt tggataaatc tgttgttctt gcgcaagcaa atgggccaat gtatgttttg
    12541 tttcaaatgc tacagcttcg tctagatcag cccatgcaat ggctcgtgca tcttgtaccc
    12601 caggaaaatc tcgacctggt tcaatatagt ctgctacata aatgattttg tcaaggagac
    12661 tcatcttagc agccccagtc gtatgcaccc ggattgcatg aagaatctct gcatcggtga
    12721 ttcctaattc ccgctctaca aacgaagcgc caacaagacc atgccagatt gcattattat
    12781 aattcaataa ctccggatca aaaccgtctc ttacgattac cattttgaac tcctcatctg
    12841 gtcgttcttt tgcataatca tgtgtcaatg cagcaatact agctttttca ggagaagcac
    12901 cgtatttttt ggcaagagcc acggctgttt cttctactcc aagcacatgc ttgaagcgtt
    12961 gttcgctcat tctcatctgt attttttgca tcaggttttc tctagaataa gcagtatatt
    13021 cattactata gtccatattc atacaaccct tcattttgaa tataatcaat tactttatct
    13081 ggaacaaggt agcggataga acagccttct ttgatctttt gacgtatttt cgtggaactg
    13141 atgtcgatct caggtacatc tacccaaatt acaggatatg gagtatccgt tgtgtatcct
    13201 ggtcgacgta tacccacaaa gttgaccata gaagtcagct catcgatttt ataccatttt
    13261 ggcaaatatt ctaccatatc cccaccgata ataaagtaat aatctgtatc cggattgttt
    13321 tgcgtcaatt ccttcatagt atcataggtg taacttttcc caccacgagc taattcaatc
    13381 gtttcaatct gcaaaaaagg attgtcttcc accgctaatt ccaacatgtt gagtcgatgt
    13441 tttgcatcaa tcgtctgttt ctcatcaaca tgaggtggct gataagaagg catcaaaaat
    13501 acgcggtcaa gacctaaatt ccggccagct tgttcagcca tcactaaatg tgcaaggtga
    13561 acgggattga atgttccgcc caagatccct acttgctttt tcttctctaa gaattgaggc
    13621 atttcttcag gaaataattg tgaacgaaca aatgtcttag cttgcgtatt catcatggtc
    13681 actccttata ttgcttttac ttcttttgat agcctttgat acttttcttt tgtggaaggt
    13741 ttgtataata caataacacg tccaatgatt tggacgattt cgcaatctat ttctttttct
    13801 aatacttgag ctgcttcttc tgcaatctca tctgtatttt gaagtaacga tactttgatc
    13861 agttctcttt tttctaaagc ttcttcaatt tgaacgatca ttgctgaatt gatcccacct
    13921 tttccgattt gaaaaatcgg ctgaagatga tgcgctttgc tacgtaaaaa acgcttttgt
    13981 tttcctctta aactcataat tttccttctt ccttaaatca atgctttgcg tttcaataca
    14041 tctacaccct ttggcgccca gcccgcaatt actactggcg ctgttacagt gatccagcct
    14101 agaccggcaa aaacgatgtc tgttttttct ttcacagaaa actcaaaacg aacaagctcc
    14161 ggaaatgcct cgacttcatc aggacgagga ggctgtaata atccacctac atgtttttga
    14221 taaaattcgt ctgctttttc cagtttcgtt cgatgaatat ttacatcatt tgacacataa
    14281 gcaataaatg aagcacgatt gccgctgata aaatcaaatc ttgcaagtcc gcccaagaaa
    14341 agtgtttgcc cttcattcag ctgataaact ttaggtttga tttcttttaa cggtgcaatg
    14401 atccgcaaat cttttttacc taagtagtgc gccatttgat ggcgatggat aattcccggt
    14461 gtatcaatca aaaaatgccc gtcatctaaa ggaatctcga ttttatccaa ggtcgttcct
    14521 ggaaactgcg aagttgtgat tacatcttgt actcccgctg tatttttgat gatttgattg
    14581 ataagcgtcg attttccgac atttgttact ccgacgatat agacatcccg tccatcgcgg
    14641 tattcttcga ttttttctaa tagttccgtc atctcatgag atttcttagc actagttaat
    14701 aatacatcta ctggacgaat tcctgcttcg taagcacgtt ccttcatcca ttgtgttaat
    14761 ttagtttttt tcaatgattt aggtaatata tccactttgt tcccaactaa aagtactgga
    14821 ttgtcaccga caaaacggtg tagtcctggg attagtgaac cattgaagtc aaaaatatcc
    14881 accacattga cgatcaatgc atctttttgc cctaattcat tcagtaagcg caaaaagtcg
    14941 tcatccgtca atgagacatc ttgggtctca ttataatggc gtaatctgaa acaacgctga
    15001 caatagactt ccccggaagc taaacctttt tccaaagccg tttgcggcgt gtatcctaat
    15061 tcaccgggtt tgtttgtttg tataacggca ccacagccga tacattgtaa ttcttctgtc
    15121 attcgattcc acctcgccag cccatatcag ggtgttttgc taatagatag gccataattc
    15181 ttttttccca aaaacgatta aattgtgtct tccaactatc tgttgcgata atgggtttta
    15241 caaggatgct acgtatccct gcacgattgg ctccacgtat atcagtcata atctgatcgc
    15301 ctaccattac tatctcatca tctgctaaat ccaattgttt ctttgctatg ttgatccctc
    15361 ttgctaaagg tttcattgct cgtgcaacat acaaaagatc aaatttttca atcgctcgcg
    15421 cgacacgttt tgaattattg tttgaaacaa caacaacagg gattcctgca tttttcattt
    15481 ctaaaatcca agtaagcaac tcttctgttc catctggatt attccatgca atcaacgtat
    15541 tgtctaaatc ggtcaatact gctttgatgc ctagattctt taattgggca ggtgtaatct
    15601 gatagatggc ttctaccatc catgttggtt tgtaatttga aaacatgctg tctcctcatc
    15661 tgcaaaaaat gaaggagcac ataaagtact cctcaacccc gtattatact gcatatcagc
    15721 aaaaaaaact acctctatac tcgttcgtct ttcagaaaaa gcatttcttt acaaaatgac
    15781 caaaaataaa gattgatcac agaaaaattt agaaacaaaa aacaactaat attggcggaa
    15841 cctcttgtat cgtttttcca gtaatttatg ctgatctaat cgaacaagtt ccgtcaatct
    15901 ttcaatcaac gcttttttca taatgtgtgc gactttatat cgtggcaact gctcgccgtt
    15961 gaacgtttca ggaataactc gatcaacgat tcctaactcg cgtagttctg gcgcagtaat
    16021 tttcatcaac tcagctgctt ctgcggcgcg actaccgtct ttccacaaaa tcgacgcaaa
    16081 tccttctgga gaaagcacag cataaatcgt ttcttccagc atccagactt catccgctaa
    16141 acctagagca agggcaccgc cacttccacc ttcaccaatg atgatggcga tgatcggtac
    16201 ctttaaatca gacatttcga gcagatttct ggcaatagcc tctccttcgc cgcgctcttc
    16261 tgctcctaca ccgcaatatg cacctgctgt attgatcaag gtaatcaccg gtctattgaa
    16321 cttttccgct tgtttcatca gtcgtaaagc ttttcgatag ccttcaggat ggggagaacc
    16381 aaaatttctg cggatatttt cctgcagatc ccgtcctttt tgaataccga tcactgttac
    16441 cggtttgcca tctaatcgag cgattccacc aacaatcgct tcgtcatctg caaataaacg
    16501 atcaccatgg cattcctcga aagaatcgaa gatctcttca aaaaaatcca agcttgtcca
    16561 tcgttcttta gctcttgcta actgaacgac atcatgtgct gtcttatcca cgatctatcc
    16621 atcctttctg tgtatgcaat tcaagaagcg tatggatttt ctgtttcaat tccatgcggg
    16681 gaacaatctg atcgacaaaa ccatgagata ataagaattc agccttttgg aaatcttctg
    16741 gaagttcctg cttgatcgtc tgttcgatga cgcgtcttcc agcaaaaccg atcaagcttt
    16801 ggggttctgc tagaataata tcgccttcca ttgcaaaact ggcagttact cctccggtcg
    16861 tcgggtccgt taaaacagtt agataaaaaa gaccttcatt gctatgccgt ttgacagcag
    16921 cagagatttt tgccatctgc attagagaaa agattccttc ttgcatccgt gcgccacctg
    16981 aagctgtgaa caagacgact ggaagacttt cctttgttgc tcgttcgaat aatctggtga
    17041 tcttttctcc gacaatcgtt cccatacttc ccatgataaa attaggatcc atcactccaa
    17101 taatgaaggg aatcccctga atggttgctt taccagtgag gactgcttca tgaagtcctg
    17161 ttttatcttg cattttttta attttttccg tatattcggg aaatgctagc ggatctttgg
    17221 ttacaagatc cgtatcccat tcttcaaaac ttttttcatc aataatcaaa gctagacgct
    17281 gccatgcacc gattcgaaaa gcgtatccgc aatgagggca tactttttct tcaccgatgt
    17341 ctttcgtata aaggatatgt ttgcaatttg gacactttgc ccacatgtta tcaggaacag
    17401 atggcgagtt agcaggtttg ctctctctat taggattgat ccgaatatat ttctttttct
    17461 taaacagagc catctatgcg cctcctatct ccgtttctgg tgtccaattc ggtaaaaatt
    17521 cttcttgtaa aaatgctgtg ctgtaatcac ctgctatgac tgcaggatga ctaatcaaat
    17581 ccatctgaaa ttctgcgttc gtgatgattc cttctgtgat aaattcactt aaggctcgct
    17641 gcatcttcat taatgcttca aaacgcgtat ttccatggac gattattttt gcaatcattg
    17701 aatcataata tggcgggatc gtatatcccg ggtaaactgc actgtctact cgcagtccca
    17761 tcccacctgc tggaagcagt aagttttgga tcttccctgg agaaggagca aaatggaaag
    17821 caggattttc tgcattaatc cggcattcga ttgcgtgtcc ttggaatact acttcttctt
    17881 gacacacatt caaaggctca ccggctgcta tctccacctg tttttttact aaatcgattc
    17941 ctgtgaccat ttctgttact ggatgttcca cttggattcg agtattcatt tccatgaaat
    18001 aaaaatcgcc ttcttcatcc atcagaaact cgatcgttcc ggcattttca taattgactg
    18061 ctttagctgc tcgaacagca gcgtctccta gtgcttctct ttttgtttgt gagatcacta
    18121 cagaaggaga ttcttccagt actttttgat tgttgcgttg taaagaacaa tctcgctcgc
    18181 ctaaatgaat gacatgacca aatcgatcac ctaagatctg aacttctata tggcgagcag
    18241 gataaatgat tttttccaaa tacatatcat catttccaaa tgccgctgct gcttcttgct
    18301 gagctgaaga aaaatgctgt gggagctctt cttttgattg aactttccga atgcctttcc
    18361 cgccaccacc tgcagctgct ttcagcatca ctggataacc aattctgtct gctatttcca
    18421 atgcttcttc tactgtagat aatacaccag tacttcctgg aatgactggg acatttgctt
    18481 cttgcatcaa ttgacgcgca ttgatcttat ttcccattgc atcgatcgtt gcacttttag
    18541 gaccaataaa tgtgatattg cactcttcac acatggaggc aaatcggcta ttttcagata
    18601 aaaaaccaaa tcctggatga attgcttcgg cttttgtcac gattgcagcg ctcagtactt
    18661 cctgaacatt cagatatgaa tcaacggctt ttgccgggcc aatgcagatt gcttcatctg
    18721 ctaactgtgt atgaagtgct tcggcatctg cttctgaata tatcgctacg gtttggatcc
    18781 ctagttcgcg gcatgcacga atgatacgta cagcgatttc accgcggtta gctatcaata
    18841 ctttagaaaa catatttcac ctatccaatc ataaaggtta attccgcttc ggctactttt
    18901 ttaccgtcta ctttagcgat tcctttgcct attccggctg aagctttcac tttcaatatc
    18961 tcaacttcaa ggtataaggt atctcccggt gtaactttct tacggaattt tgctttgtcc
    19021 aatccgccga aataagccgt ctttcccttg aattgttcaa gagaaagtaa tgcaactgct
    19081 cctgcttgag ccattgcttc aacaatcaat acccctggca taacaggttc ttctggaaaa
    19141 tgtccttgaa aaaagggctc attgatcgtt acatttttct tagcaacgat ccgttctcct
    19201 tcaaccattt cttccactcg atcaatcaaa agcattggat aacgatgtgg aatgatttct
    19261 tttatttctt gtatgttcat tgttttccct ctttcctgct ttgcttattt ttgctgaatc
    19321 ctaaagagtg gctgtccata ttcgacaact gactcgtttt ctacaaggat ttctgtgatc
    19381 actccgtcgg cctcactagt tatctcattc atcagtttca tcgcttcaat aatgcagata
    19441 acttctcctt tggcaacttg atcacctact tttttgaaag ctggtttgtc gggcgctggt
    19501 tgcagataaa cgattcccac gattggcgaa acaacctcga ttccatcaaa tactgccgat
    19561 tctttttctt tcattatttc tggagcagtt gttgcttgtt caggtatgac attgactggc
    19621 tccgaaactg gcaacgtttc gtttgtttga accatattca tgcctcgact tgttttattt
    19681 ttattcatat acaattcaaa ttgaccttca cgcaaatcga attccgttag cgaggattga
    19741 tcaaattgac tgactaattc tctgatttca ttgatatcca aaattattcc tcccagcgtt
    19801 tcatgcacag aaccgcatta tgtccgccaa accctaatga attactcatt gcataagtaa
    19861 aacttgcagg tttgctttcg tttttgacaa ctgtcaagtc aatctcagga tcttgttttt
    19921 ctacattgat cgtaggtggg ataaattgat gttctaatgc cagcaaagtt gccaaggctt
    19981 cgatcccgcc ggctgctccc aataaatgtc cagtcatcga tttcgtgctg ctgacgtaag
    20041 tatctctgaa attttcacct aatgcgattt ggattgcttt tgcttctgcc tggtcattgg
    20101 ctggtgtact tgttccgtga gcattgatat atccgacttg ttccggctgt acaccagctt
    20161 cttccatcgc aagcttcatt gctttagatg caccagaacc atctggagta ggagctgtca
    20221 tatggtaagc gtcacagttt gcaccgtatc caacgatttc tccatagatt ttggcaccgc
    20281 gagcttgtgc atgttctaaa gattcaagaa ctagaacgcc tgctccttca cccatcacaa
    20341 aaccattccg attttcgtca aacggaatcg aaccacgatt aggatcagtt gattctgaga
    20401 gtgctgttaa agctgcaaat ccagcaatcc ccatctcaca aatcgttgct tctgttcctc
    20461 cagcaagaat gacatcagaa tagccatgtt tgatgttacg gaatgcttcc ccaattgaat
    20521 tcgttccgct agcacaagct gttacgatag ctgtacaaat accttttgca cctacacgca
    20581 aagcaatatt tccagctgcc atgttggcaa tcgccattgg tacgaacatt ggagaaactc
    20641 gttttgcacc tttttcatgt agacgaataa cttggttttc gattgttgga agaccaccga
    20701 tcccagatcc gatgatgacg ccaaaccggt cttgattcat tttttcttta tctaaaccgc
    20761 tcatctccat tgcttctaaa gcagcgtaga ttccatagat cgaaaacata tccattcgtt
    20821 tgctatcttt tttaataaaa tatttatcgt aagggaaatc tttgacttca cccgctaagg
    20881 taatccctgt ttctgaagca tcaaatttag tgatcggtgc gatgccattc gtcccctctt
    20941 ttaagctatt caaaaagctg tctgcgtcat ttccaatagg tgaagtgact ccatagccag
    21001 taattaccac tcgattcatt tgtttgctct ccttttctta tccgtgcatg accaatccgc
    21061 catcgacatt gatgacttgt ccggtgatat atggattttg tgctaagaaa actgctgttt
    21121 gtgcaatatc ttctacttgt ccgaaacgct gcatcggaat ttgcttttcc gcttgttcct
    21181 tgactttgtc tgctaaaact tccgtcatat cagttgtgat gaatcctggc gcaatggcat
    21241 tacatgtaat gcctctagtt gcagcttctc gtgccacaga ttttgttaat cctactacgc
    21301 cagctttact agctgcataa tttgcttgac caatatttcc aatcaaccca gatacactag
    21361 agagattaat gatggcccct tctcgctgct tcatcatttt tttcaatacg tgctgcgtca
    21421 tattgaaggt tccaatcaaa ttgatatcca gacatttttt gaaatcttct gcatccattc
    21481 gcatcacaag tttgtcattt gtgattccgg cattgtttac taacacatgg atcgatccaa
    21541 gcttttcttc tgcttctttg atcatttgtc ccgctttctc ataatcagag atgtcaccgg
    21601 aaactcctac gcattttaca ccgaatgctt cgatttcttc tatcttttct tttggaatct
    21661 ctccgcgacc attcaaaatg atatttgctc cagcttttgc aaaagctaag gccatcgctt
    21721 tgccgattcc tcttgtacta cctgtgataa atacattttt tccttttaca tccataacgt
    21781 tcctccttat agtattgcta ttgtttcatc aaatgtctgc ttattctcaa cacgcagaac
    21841 tggtattgtt ttatcgattt ttttcataaa tccgcttaat acttttccgg ggccgacttc
    21901 gatgacttgt tcgatgccga ttgtttttaa cttatggata ctctcataga aacgaacagg
    21961 ttgcatgact tgctgttcaa gtagtcccgc gattgtttct ttcttcatta tttccgttgt
    22021 tgtgttgctt atgataggaa aagaaggttc agagaattga atctgactta ggtccttcgc
    22081 cagttttttt gctgccggtt ctaaaatagc tgtatgaaaa ggcccgctga catttagagg
    22141 gatcattctt ttgaaccctt tttcttttaa tagtaaaaca gcttcgtcta ctgctttttc
    22201 ttcgccacca atcacgatct gctgaggtgt attataattt gcaggagata cgatgccgta
    22261 cttgctagct tcatgacagc tttcctcgat cgtttcaatc ggcgcattca tcactgcaac
    22321 cattttcccg cttccagctg gggcagcttc tgtcatataa gcgcctcttt ttgctactaa
    22381 cgcaactgct tcagtgaagg atagcgcatt actagctact aatgccgaat attctcctaa
    22441 actcagtcct aaagctgcat ctgggatgat tccatgctcc ttcaataagc gataatacgc
    22501 gatgctgact gttagaatag ctggctgtgt atattgcgtc tgatccaaac gttcattttc
    22561 tgtaaagcaa agttcagcca tatcgtagcc gagaatctca cttgcttcat caaatgtctg
    22621 ctttacaacg gcttcttcat ataaatcttt tcccatacct tgatattgtg caccttggcc
    22681 actaaataag aatgctgttt tcatgttttt ctccaatata tccagaatat atgtttgatt
    22741 cttcctattc aaaaaggccg agacattcct atttcaggaa atgtcccagc ccccaaccta
    22801 attttaatga ttatgcagtt tgtttttcta cgtaagtcac taaatcttga accgttttga
    22861 ttccgtcttc tgtttcgatt ttcacgtcaa attcatcttc aatttcattg atgatttgga
    22921 ataaatccaa gctgtcagct tctaagtctt cttggatgtc agtagtcaat ttgatttctt
    22981 cagtttcttt tcctaattct tctgcgatga tttcttgaat tttttcgaat accataataa
    23041 tatattcctc cgatattgtt tgctttttat ttttataatt taacagtcta caactccatg
    23101 agaatacttc cccatgttaa acctccgcca tagcctgtaa atattacttt ctgctgtttt
    23161 cctaaggtga tctttccact agaaactgct tcatccaata agatagggat gcttgcagca
    23221 gatgtattcc catatttatc catattggtc aggaactttt cttgcggcat ctttactttt
    23281 cgggcaattt tctcaataat tctgaggttt gcctgatgca aaagcagata atcaactggt
    23341 ttgtctttca tgacttcttg gatattgttt gcaacgtctc gtacggcaaa atcaaaaata
    23401 tctctgccag tcatttgcaa ataatacgaa ccttctgaat cttctgaata gaacggattt
    23461 ttattttgct ggtagccagc ggtcaatgag ctacttctgg taccgtcagc tgctaatttt
    23521 tctttcagca attttttctg tggattcgct tctaagagaa tcccacctgc tccatcgcca
    23581 aacaacacag cagtcgtacg atcagaccaa tcgattagct tagatagtgt ttcaccacca
    23641 atcaccagtc ccttagttga gccgcagcgg atcatttttt cagccatgct taacgcataa
    23701 acaaatcctg agcacgcagc actgatatca aaagcgatag catgagttgc tcccaatttc
    23761 ccttgtactt gtgctgctac agatggtgtc gtgtaatcgg gcgacattgt tgctactaaa
    23821 ataaaatcca aatctttagg gtttagattt ttctgtttta tcaatttttc cgccactaaa
    23881 atgcataaat cagaagtctc ctgatttgtt acacatcttc gttcacgaat acccgtcctt
    23941 gacgaaatcc attcatcaga ggtatccatc atcttggaaa gatcattatt agtaataatc
    24001 ttttcaggaa ggacgctggc tgttgcagta atcattccaa attgttccat taaatatcct
    24061 cacttatagt cttgcaagaa gtcgtgcaag tttttcagtg catccagcaa ggcatgctgt
    24121 tcctccgtag ccattccgtc taagatgttc ttgaccattt cacgatgaaa atgctgatgg
    24181 acccgaaata gtaactttcc ttttttcgtt agtcccaact ttacaacgcg gcggtcatct
    24241 tcactgcgga tacgttcgac atagcctttt tttactaact tgttaatagc agtggtcaag
    24301 gtcccaacag taattgatag ttctttggct acttctgaag ttgttttttt cttgtacatc
    24361 ccgattgcct cgatcgtgtg catctctgtg attgacagat ctttgaattg agattttttc
    24421 agctcagact cttcgattgt caaaatatca ttgaagacac taacaagata atcatttact
    24481 gtttttaaat ctggttccat gttccaccgt ccactaatta gtttgattat caaatctttt
    24541 gatgttcaaa ctatatctta acttttttca aaatgcaacc ttaaaagatc atttttttac
    24601 tatatgagct ttttttaaga ttttactcat ttaaaacaac tgataaatca gcatttataa
    24661 tttcttttat tagaaaaatc atctatctca catattttga ttatcaaagt attttctgtg
    24721 aacagaacta tagaattccc taaaacttgt ccattatttc aaaatttgtt atagtctttt
    24781 tattcaaagc gtaaaaggag atcctatgtt aaaagaaaaa atgaaaactc tccccatgct
    24841 gccgggtgtc tatcttatga aagaccgaga tggtaaggtc atctatgttg gtaaagcaaa
    24901 acggctcaaa aatagagtaa gcagctattt tcatcagaat aaacagcatt caaaaaaagt
    24961 attacgtatg attcatcata taacagattt tgatttcgtc gtagtagaca cggaattaga
    25021 tgctttattg ctcgagtgtc aattgatcca acattaccgt cctttttata accgacaaat
    25081 gaattatttc agtaattata attatgtaca tattaccaat aagggatttg tattaacaga
    25141 tactcctacg gctcgaacat acgggccttt tcgtctctat aaaaaaatgc cgtctatttt
    25201 gcgtataatg gaagaaactt atcaaatgcc ttggttgtct gaaattagtt tgttagcttt
    25261 gcgagtccag cttcctgacc tacaggaaat gacttttgaa cagaaaaaaa aggaactcca
    25321 agggctgttt caaggccgaa ataaaaagct actgacttac ttgaaaaaaa gacaacaaca
    25381 tttcatctat cagttgaatt tcgaaaaagc cggtatgctt caaaaagata tcgaattagt
    25441 tacttatttt attagacgga tccaggaaca aaagcagttt ttaagaacac catcgctcac
    25501 cttcagcatg ccattagcag cagatgagag tcagaaaaag cactacctcg tttgttatgg
    25561 tcaacttgca gaaacaatga ttacctctgg tgacgtttct cctgacttct attatgaaaa
    25621 gaaagaagct catttatctt tgaaacgtca attgtcaaaa gaagaaatcg atcctgtaca
    25681 aatcttgatc agttaccgca aaaaattaga aaaagaacag tttgaaattg aactgttaaa
    25741 caaaaaagaa gctgaaaagc aactaaatta gttgcttctc ggcttctttt aggcatcttc
    25801 aacttcaaat gtcaattatc ctaaaattta ttggattaag tcatagattt ccatcgctac
    25861 gatatcgata ttatcaaatt cgtattgctg atgagtatct gtttcagtta attcaaatgt
    25921 atcggtcgct ttatcataag ttactgtaca cttctctacg ccttcttttt caaaacggcg
    25981 aacttgcagt tcattatctg ctgactcgac catagcctcc agtcgtttta tgattgcgac
    26041 taattgtgaa tgtgtcatcg gaggtcctcc ttttcttatt tctgttatat tgtatcaaaa
    26101 gcatagccta cttgcattga ttttctctaa aaaaactcgt tttttatgat tttttttctg
    26161 gtatttttcc ttcaggccac caaagtttga tcaaagcttg ggttccgtca ttctcaaaac
    26221 ctttatctgc caactcttca tatagtttca atgcttgggt agttgctggc aatacaagat
    26281 ccattttttc tgcttcttct aatgcaatct ttaaatcttt gataaaatgt ttgacaaaaa
    26341 aaccaggtgt gtaatcttct tttaaaatac gaggagaata attgcttaaa gaccagttag
    26401 ctgcacttcc acctgaaagc gtctcaatca ctttctttaa atcaagtccg tttttttgtg
    26461 catagacaag catttccgtc attcctgtca tcgtacctgc aatcatgatc tgattagcca
    26521 tttttgtatg ctgacctttc ccagcagaac catgcaatgt aaatgtcgta ccaaattctt
    26581 tgaataaagg cagtacttga tcataaacgg cttcttctcc accgaccatg atcgtcagtg
    26641 taccattctt tgctcctaag tcaccaccgg ataccggtgc atctaacgca tctgcgcctt
    26701 tttcttttgc tgtttttgca attttttctg ccaggcttgg tgtacttgta gtcaaatcaa
    26761 ctaaaatatg tcctgagata tctgcagaaa aaatccccga ctgtccatag tagatttctt
    26821 ctacatcttt ggggtaacct accattgtaa agagaatatt actttgttct gctaccgctt
    26881 ttggtgtatc tgcccagata gctccttctg ttactaaagg atcagctttt gcttttgtcc
    26941 gattatagac agctacttcg tgtcctgctt ctagtagatg gcgtgcgaca gcacttccca
    27001 tgacgcctgt tccgataaaa ccaagtttca ttttttcgac ctcctaaaaa tcaaaaatcc
    27061 ttgcttctat gctagtatac gctagaaaca aggatttttt cataaaaaat gtttgaattt
    27121 tatcttttaa atgaatttaa ttttattttg attcgttcaa tcgagtcttc ttcttctttt
    27181 cttcgcatct cttcttcatg ataaacaacc atgtcttgat aaaattcgat catagcttcg
    27241 ccgaaatgtg tagatgaaat atcataaagt ttagcttcta aaacttttgg atctttagga
    27301 acttcactgg aaatataatc aataagtgct ggtgcaaagt cttcgtcggt ttcaaaagtt
    27361 ttccctaatg aaggatgatc gaacaatcgg ttcaaataag catttccttc cacgacacaa
    27421 ggtgttccgg ctgccatcgc ttctgtataa gtaagccctt gtgtttctga agtagaagca
    27481 ctgacaaaat aatctgccgc tttataataa agtgctacat cattgttgtc cacttctcct
    27541 gtgaactgca caaattcttc taactgcagt tctgagatca attcctctaa ctcttctaca
    27601 taaggtcctt ttcctacgat aactaatctg gcgttaggat aagcggagat gacctgagga
    27661 aacccattaa tcaaggcttg gatatttttc tcataagaaa tcctgcttaa agaaagaatc
    27721 atcaaatggt tttcctgaag acctagtgac tcccttaatt tgctgatatc ctgctgagtg
    27781 atatcagaac gtttgaattt ttcaatgtcg ataccagtag gaatcacgcg aagcggtgct
    27841 attacaccat agctgcgtaa tgtatcaata acacgttcac ttggacaaac tacacctgtc
    27901 gtatgatttg caaataatct agacaagtat tttacatggg attgcctgat cacttttcct
    27961 ttaccgatat aatgaaggta gtcttcatac atcgtatggt aagtatggat cagaggtatt
    28021 ttcaacttct tagctaccat cttcccaagt atccctgctc cgaattctgt atgggtatga
    28081 ataagatcta gttctagttc tttggcaacc aaataagcat accacatgcc tctaacaaca
    28141 actcttcgat ccttgaaaga aataaatggt acacttggca ttcgaatcac atctttttcc
    28201 aacttatccg cattaggatc ggtcgttgta aaaatataga cttcatgccc atgtctttct
    28261 aattcttgtt ttaatgtttt gatcgatgta gcaactccgc tgacttgcgg aaagtaggta
    28321 tcggtaaaaa agccgatttt catgtttttc gcctccaaac tagtccttga cctgatttac
    28381 tttttttaat ttgctagaga ctttttcatt cagtacactt tcgtatacat ttttaagttc
    28441 ttcaccgatt ttattgatac ttcgctcttt tgctgtttga taaccggctt gtgtcgtact
    28501 cggtagttct tgattggtaa tctgttggat cagtcggata aattcttcgt ttgtcttccc
    28561 catatagcag ttcttgctat cttctaacca gccattatac actggaatat ctcgaacaag
    28621 tacgttttgt ttgcttgcta aagcttctaa aacaacgatc ccttctgtct cttcataaga
    28681 agggaaaaag aatagatctg cacctgaata cgcaccttct atgatctctc cacgaatgta
    28741 accaggaaac aggacatttt caggatgatc ttcttttaca attttccgga tgttccgagg
    28801 aatcgagtac atgggaacat gaccaaacca aataaatgtg tattcgggca tcttcttagc
    28861 aatttctacg aaatcaatga ttcctttacg ttcgaaaaac aaaccgacac aaatgattac
    28921 tttttgatcc ggagacagtt tgaaatattc tctgaacttt tgttctttca ttggatcagg
    28981 gtgatatttc tctaagtcga tcccattgga aatagcttga atcggcacac ggataccata
    29041 gctttccaac aactgttttg aataaggtgt tggcgtgatc agatagtccg ctttttggta
    29101 caatcctacc agatattttt ttacgatagg agaaagctgg ttcgatccga taaaggaatt
    29161 tcggaaatct tcctctgtag aatgcgcatg atagacaact ttttttcctt ctcttctggc
    29221 tttattgacc atattatgac tattgattcc gtatgtattg atgtgcagga tgtcataatc
    29281 ttctttttca tctagtgtat aagagatacc gacttccgtc agagcacgtt tttgatgatc
    29341 aagcgcacgc ccgattccag attttgcaag catcttttca ctttcaaaat ataataatac
    29401 cttcacctta cgtccccctt cacttatata gtataacata gcctacaaac tgtcactcaa
    29461 acttaagccc tatttttgtc tattcttact gacaaaaacg ttttcttaaa aaagaacaaa
    29521 aaaactggcg tagctctttt acaagccaca ccagcttttg atacgttgtt tattttacat
    29581 attcatgaac aagttcaacg acttcttcca tcgtatcgca ttctttcaat gcacgatcag
    29641 caagttcagc cattttcgct gtgtccaagc gtttcattaa accacgcgtt ttaagaatag
    29701 atgtcgcact cattgagaac tcatctagac ccatacctac tagcaatgga acagctgttt
    29761 gatctccagc catttctcca cacatacctg cccattttcc ttcagcgtgt gctgcatcaa
    29821 ttacattttt gatcaaacgt aaaattgatg ggttatatgg ttggtacaag taagaaacgc
    29881 gttcgttcat acggtctgct gccattgtgt attgaatcaa atcgttcgta cctacagaga
    29941 agaaatctac ttcttttgca aacttgtctg ctaagactgc tgctgctgga atctcgatca
    30001 tgatgccgac ttggatcgta tcagatactt ccttgccttc acttatcaat ttttgttttt
    30061 cttcttcaaa gatcgctttt gctgcgcgga attctttgag tgtagctacc atagggaaca
    30121 tgatacgtaa attaccatga acagatgcac gaagcaatgc acgcatttgt gtacggaaca
    30181 tttcgtcgcc ttgttcagat aaactgatac gcaatgcacg atatcctaag aatggattca
    30241 tttcatgagg taattgcaga tatggtagtt ctttatctcc accgatgtcc attgtacgaa
    30301 caactacagg ttttccttcc ataccttcta atacagcttt gtaagcttca tattggtcat
    30361 cttctgttgg gaagtctgga gaatccatat acaggaattc agtacggtac agaccaactg
    30421 cttctccacc attattatgg acaccaacta gatcttttgg tgttccgatg tttgcagcta
    30481 attcaaaatg cttgccgtca gctgtgacag tttctgcatt ttttaatttt tcccattctg
    30541 ctttttgtgc tgcgtaatct gcacctgctt tttcaaattc agctttttgt tcatctgtag
    30601 gatcgatgat tacatcgcct tcgatcccgt tgacagccaa aatatcgcct tctttgactt
    30661 tagcagtaat ttcttttgtc ccaacgattg ctggaatttc taaagaacgt gccatgatag
    30721 cagaatgtga agtacgtcca ccaatgtctg ttacaaacgc ttttacaaaa ttgcgatcta
    30781 attgtgctgt atcacttgga gtcaagtcat gtgcaacaac gaccacttct tcgttgatca
    30841 ttgatggatt tggtaaagta acgcctaata agtgcgctaa aatacgttta gcaacgtcac
    30901 gaatatctgc agcacgctct tgcatataag cattatcttc cattgcttcg aacataccga
    30961 tatacatatc cgttacttct tttagcgctg attcagcatt tacgctgttg tctttaatat
    31021 tttgcttgat ttgacctacc atttctggat ctgaaagaac catcaaatgg gcatcaaata
    31081 cttgcgcttc tgcttcacct aagctttgcg ctgctttttc acgaatttgc tgcaattctt
    31141 cagtagattt agctaatgca gcatccaaac gagcttcttc agccgcagta tcttctactg
    31201 ttgccttact gaatgttaaa tccggttgaa ctagcaggta agcttttgca acggctacac
    31261 cgtcactagc ggcaatccct tttagcattt caaccattat tcagataagc cttctttctt
    31321 cattgtttca acgattgctg ccattgcatc agcttcgtca gcaccttctg cagtaatagt
    31381 aacatcagaa ccttgaccta cacctaaaga cataacgccc atgattgatt tcaagtttac
    31441 tgatttacct ttatactcta agtttacatc agagttaaat ttgcttgctg tttgtactaa
    31501 tagtgtagct ggacgagcgt ggatcccagt ttctgctact acgtgaaatt ctttcttttc
    31561 catattaatc ggtctccttt aagtgagtgt tgtttttatg tgagggtttt cttttttagt
    31621 aattcagaga atcctaacaa atgccaccct ttcatctgta aagattaaca ttttttttcg
    31681 ttagatacaa cagttttcat tcaatttcag ataaaagtat tcaaattatt ccggattctt
    31741 tttcatcctt ttcttcacat tttataacaa acgtgtttat ttcctttttc ttgtcgcttt
    31801 ctcttctcct gacgtttatc attctttcct ttgaccctct ttgttttcat atgaaacgaa
    31861 ttacttgctt ccctttttat ttttagtata ataagagtca aaggtcagga atggtcaata
    31921 gagatttcac cttttctacc tcataaatct cttaaaaaac taggggttat tcttttacct
    31981 catgaaaaac gagatgtaaa atatggatgt acccattttt gtgaaaggac gtgaatgatt
    32041 catgctttgt caaaactgcg gcaagaatga ggcaacgatt cacttgtatg ccactgtcaa
    32101 tggtcagcgg acacaactcg attactgtca gagctgttat caaaaaatca aaaatcaaca
    32161 aaatggaggt ctgactaata tggcccaaaa tgatccattt ggatttggca gtttggacga
    32221 tcttttccgt tccatgtctc gccaaatgca acagcaagga aattatgagc aaacaccacc
    32281 aacacaattt ggcggaaaca acaacttcaa tggtggtcag ccacctcaag gcaatacaga
    32341 aggtttattg ggagaatacg gaatcaatat cacagaacaa gcacgacaag gtgatatcga
    32401 tcctgtgatc ggacgcgatg acgaaatcaa acgagtcatt gaaattttaa atcgccgaac
    32461 caagaacaat cctgtcttga ttggagaacc aggtgttggt aaaacagcag ttgttgaagg
    32521 tttagcacaa aaaatcgtag atggtgatgt accgcaaaaa ttaatggaca aagaagtcat
    32581 tcgtttagat gtcgtatctt tagtccaagg tactggtata cgaggacaat ttgaagaacg
    32641 tatgcaaaaa ttgattgaag agatccgtca agcagaaaat gtgatcctat tcatagatga
    32701 agttcacgaa atagtcggtg ctggttctgc tggtgatgga aatatggatg ctggtaatat
    32761 tttaaaacca gctttagctc gaggagaatt gcaaatggtt ggtgctacta ccttaaatga
    32821 gtaccgcatc atcgaaaaag acgctgcttt agaacgtcgt atgcaacctg tacgggtaga
    32881 tgaaccaact gtcgaagaaa cgatcagtat tttgaaaggt ttgcaaaaac gttatgaaga
    32941 ctatcatcat gtaaaatata ctgatgaggc catcgaagca gccgcaactt tatccaatcg
    33001 ttacgtccaa gatcgcttct tgccggataa agccatcgat ttgctagatg aaacaggttc
    33061 taagaaaaac ttgacgatcc aaatcgttga tcctaaaaca atcgagaaaa aactgcaaga
    33121 agcagaagaa caaaaagttt tagcttctcg agaagaagat ttcgaaaaag cagcttatta
    33181 tcgcgatcag atcaataaac ttcaaaaaat gaaagaacgt cagctcactg aagaggaaac
    33241 acctgtgatt accgaaaaag atatggaaaa aatcgttgaa caacggacag gtattccagt
    33301 gggtgaatta aaagaaaaag aacagacaca actgaaaaac ttagcagatg acttgaaagc
    33361 tcatgtcatc ggacaagata acgctgtaga tcgagtagcg aaagcgattc gccgaaaccg
    33421 tgttggatta aacaaacaga atcgtccaat cggctccttc ttatttgttg gaccaactgg
    33481 tgtaggtaaa acagagcttg cgaaacaact tgcctacgaa ctattcggtt cacaagattc
    33541 aatgattcgt ttcgatatgt ctgaatatat ggaaaaacat agtgtctcta aattgattgg
    33601 ttctcctcca ggttatgtcg gatacgaaga agctggtcaa ttaacagaaa aagtacggag
    33661 aaatccttac agtcttgtct tgttagatga agtagaaaaa gctcatccag atgttttgca
    33721 tatgttcttg caaatcttag atgatggtcg attgacagat gcacaaggaa gaacggtcag
    33781 ctttaaagat acgatcatca ttatgacaag taatgccgga actggtaaag tagaagcaaa
    33841 cgttggtttt ggtgcggcac gcgaaggtgt tactcgttct gtattgaacc aattaaacaa
    33901 ctactttaca ccagaatttc tgaaccgttt tgacgggatc atcgaattca gtgcattatc
    33961 aaaagaaaac ttgatgacaa tcgtcacact aatgctagac gatgtcaacc aaatgctggc
    34021 agcacagcaa cttcacatcg aagttcctac gaatgtcaaa gaaaaattag ttgacttagg
    34081 ctatgatcca tctatgggtg ctcgtcctct tcgacgcacg atccaagaac aaatcgaaga
    34141 tggtatcgct gaattttatc ttgatcaccc ttctatccat gagttgaaag caaaactgga
    34201 taaagatgga aaaatcatcg ttacttctaa accagaacgt ttggctaaag aatcagccga
    34261 agaaacggct gaataaatcc agttttatag cgtgagacgg tatttctttg agaaatgccg
    34321 tctcattctt ttttcagaaa agtcaaaata ctgttcattt tataaaaaag aaattataat
    34381 aagagaaaca agcaattaaa aatacataaa agaggtgagc gccatgaaat tggtaaatgt
    34441 aacaaacagc cattcccgtc ttgtattaaa ccagcttgaa aacactgatg cccatctagt
    34501 caaggtctac acagccggaa ataccactat tgtctacact gaagcccctc aacataatga
    34561 aattctgctg atcaatgaca aacgtaaaat ccagccgaaa gaaatcgaag atgctaaaaa
    34621 ttatttctta gataaaatca aagacgccgt ttaccacgag gatgaaatca aagtaatcga
    34681 attggcaggc cttgttgaaa tctcgattcc aaaacatgaa ttaggcgcta cattagccgt
    34741 gtaaacaaat tttgaacata ataattaaag agcgtgggac aaaagtaaa
//
LOCUS       DAZUKC010000023        34549 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000023 DAZUKC010000000
VERSION     DAZUKC010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.024"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..604
                     /locus_tag="ACG80T_001056"
     CDS             212..604
                     /locus_tag="ACG80T_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS4840723.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="ACG80T_001057"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="ACG80T_001057"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFS4840724.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            2698..2943
                     /locus_tag="ACG80T_001058"
     CDS             2698..2943
                     /locus_tag="ACG80T_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840725.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            3165..4775
                     /locus_tag="ACG80T_001059"
     CDS             3165..4775
                     /locus_tag="ACG80T_001059"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFS4840726.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4752..4964)
                     /locus_tag="ACG80T_001060"
     CDS             complement(4752..4964)
                     /locus_tag="ACG80T_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840727.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            5067..5936
                     /locus_tag="ACG80T_001061"
     CDS             5067..5936
                     /locus_tag="ACG80T_001061"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFS4840728.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="ACG80T_001062"
     CDS             6101..7372
                     /locus_tag="ACG80T_001062"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFS4840729.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="ACG80T_001063"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="ACG80T_001063"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFS4840730.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="ACG80T_001064"
     CDS             8763..9026
                     /locus_tag="ACG80T_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFS4840731.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="ACG80T_001065"
     CDS             9471..9920
                     /locus_tag="ACG80T_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4840732.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="ACG80T_001066"
     CDS             9977..11395
                     /locus_tag="ACG80T_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFS4840733.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="ACG80T_001067"
     CDS             11395..12255
                     /locus_tag="ACG80T_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFS4840734.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="ACG80T_001068"
     CDS             12429..13202
                     /locus_tag="ACG80T_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS4840735.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="ACG80T_001069"
     CDS             13349..13714
                     /locus_tag="ACG80T_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFS4840736.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="ACG80T_001070"
     CDS             complement(13862..15070)
                     /locus_tag="ACG80T_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4840737.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="ACG80T_001071"
     CDS             complement(15089..15367)
                     /locus_tag="ACG80T_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840738.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="ACG80T_001072"
     CDS             15560..16216
                     /locus_tag="ACG80T_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS4840739.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="ACG80T_001073"
     CDS             complement(16658..17455)
                     /locus_tag="ACG80T_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFS4840740.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="ACG80T_001074"
     CDS             complement(17452..18636)
                     /locus_tag="ACG80T_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFS4840741.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="ACG80T_001075"
     CDS             complement(18661..19908)
                     /locus_tag="ACG80T_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFS4840742.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="ACG80T_001076"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="ACG80T_001076"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFS4840743.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="ACG80T_001077"
     CDS             21888..22202
                     /locus_tag="ACG80T_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFS4840744.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="ACG80T_001078"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="ACG80T_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFS4840745.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /gene="tmk"
                     /locus_tag="ACG80T_001079"
     CDS             22887..23531
                     /gene="tmk"
                     /locus_tag="ACG80T_001079"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFS4840746.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="ACG80T_001080"
     CDS             23546..23875
                     /locus_tag="ACG80T_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFS4840747.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="ACG80T_001081"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="ACG80T_001081"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFS4840748.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="ACG80T_001082"
     CDS             24863..25687
                     /locus_tag="ACG80T_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFS4840749.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="ACG80T_001083"
     CDS             25680..26027
                     /locus_tag="ACG80T_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFS4840750.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="ACG80T_001084"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="ACG80T_001084"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFS4840751.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="ACG80T_001085"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="ACG80T_001085"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFS4840752.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACG80T_001086"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACG80T_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFS4840753.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACG80T_001087"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACG80T_001087"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFS4840754.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="ACG80T_001088"
     CDS             31684..32451
                     /locus_tag="ACG80T_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4840755.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="ACG80T_001089"
     CDS             32441..34483
                     /locus_tag="ACG80T_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4840756.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLITGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT        11111 a         6124 c         7253 g        10061 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttattta caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt ttgtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa cgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagagg
//
LOCUS       DAZUKC010000024        34390 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000024 DAZUKC010000000
VERSION     DAZUKC010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.025"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            294..983
                     /locus_tag="ACG80T_001090"
     CDS             294..983
                     /locus_tag="ACG80T_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS4840757.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            970..2178
                     /locus_tag="ACG80T_001091"
     CDS             970..2178
                     /locus_tag="ACG80T_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFS4840758.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2472..3743
                     /gene="serS"
                     /locus_tag="ACG80T_001092"
     CDS             2472..3743
                     /gene="serS"
                     /locus_tag="ACG80T_001092"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFS4840759.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4292..5776)
                     /gene="guaB"
                     /locus_tag="ACG80T_001093"
     CDS             complement(4292..5776)
                     /gene="guaB"
                     /locus_tag="ACG80T_001093"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFS4840760.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6281..6976)
                     /locus_tag="ACG80T_001094"
     CDS             complement(6281..6976)
                     /locus_tag="ACG80T_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFS4840761.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7066..8166)
                     /gene="ychF"
                     /locus_tag="ACG80T_001095"
     CDS             complement(7066..8166)
                     /gene="ychF"
                     /locus_tag="ACG80T_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFS4840762.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8175..8366)
                     /locus_tag="ACG80T_001096"
     CDS             complement(8175..8366)
                     /locus_tag="ACG80T_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFS4840763.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8387..9277)
                     /locus_tag="ACG80T_001097"
     CDS             complement(8387..9277)
                     /locus_tag="ACG80T_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFS4840764.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9264..10031)
                     /locus_tag="ACG80T_001098"
     CDS             complement(9264..10031)
                     /locus_tag="ACG80T_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFS4840765.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10043..10756)
                     /gene="rsmG"
                     /locus_tag="ACG80T_001099"
     CDS             complement(10043..10756)
                     /gene="rsmG"
                     /locus_tag="ACG80T_001099"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFS4840766.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(10988..12889)
                     /gene="mnmG"
                     /locus_tag="ACG80T_001100"
     CDS             complement(10988..12889)
                     /gene="mnmG"
                     /locus_tag="ACG80T_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFS4840767.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12906..14303)
                     /gene="mnmE"
                     /locus_tag="ACG80T_001101"
     CDS             complement(12906..14303)
                     /gene="mnmE"
                     /locus_tag="ACG80T_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFS4840768.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14602..14985
                     /locus_tag="ACG80T_001102"
     CDS             14602..14985
                     /locus_tag="ACG80T_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840769.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15153..15911)
                     /gene="jag"
                     /locus_tag="ACG80T_001103"
     CDS             complement(15153..15911)
                     /gene="jag"
                     /locus_tag="ACG80T_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFS4840770.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15929..16747)
                     /locus_tag="ACG80T_001104"
     CDS             complement(15929..16747)
                     /locus_tag="ACG80T_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFS4840771.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16744..17100)
                     /gene="rnpA"
                     /locus_tag="ACG80T_001105"
     CDS             complement(16744..17100)
                     /gene="rnpA"
                     /locus_tag="ACG80T_001105"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFS4840772.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17218..17352)
                     /gene="rpmH"
                     /locus_tag="ACG80T_001106"
     CDS             complement(17218..17352)
                     /gene="rpmH"
                     /locus_tag="ACG80T_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFS4840773.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17875..19209
                     /gene="dnaA"
                     /locus_tag="ACG80T_001107"
     CDS             17875..19209
                     /gene="dnaA"
                     /locus_tag="ACG80T_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFS4840774.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19407..20537
                     /gene="dnaN"
                     /locus_tag="ACG80T_001108"
     CDS             19407..20537
                     /gene="dnaN"
                     /locus_tag="ACG80T_001108"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFS4840775.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            20766..21008
                     /gene="yaaA"
                     /locus_tag="ACG80T_001109"
     CDS             20766..21008
                     /gene="yaaA"
                     /locus_tag="ACG80T_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFS4840776.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            20995..22119
                     /gene="recF"
                     /locus_tag="ACG80T_001110"
     CDS             20995..22119
                     /gene="recF"
                     /locus_tag="ACG80T_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFS4840777.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22116..24062
                     /gene="gyrB"
                     /locus_tag="ACG80T_001111"
     CDS             22116..24062
                     /gene="gyrB"
                     /locus_tag="ACG80T_001111"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFS4840778.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24082..26553
                     /gene="gyrA"
                     /locus_tag="ACG80T_001112"
     CDS             24082..26553
                     /gene="gyrA"
                     /locus_tag="ACG80T_001112"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFS4840779.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            26789..27088
                     /gene="rpsF"
                     /locus_tag="ACG80T_001113"
     CDS             26789..27088
                     /gene="rpsF"
                     /locus_tag="ACG80T_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFS4840780.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27138..27650
                     /gene="ssb"
                     /locus_tag="ACG80T_001114"
     CDS             27138..27650
                     /gene="ssb"
                     /locus_tag="ACG80T_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS4840781.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27676..27912
                     /gene="rpsR"
                     /locus_tag="ACG80T_001115"
     CDS             27676..27912
                     /gene="rpsR"
                     /locus_tag="ACG80T_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFS4840782.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28055..30028
                     /locus_tag="ACG80T_001116"
     CDS             28055..30028
                     /locus_tag="ACG80T_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFS4840783.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30034..30486
                     /gene="rplI"
                     /locus_tag="ACG80T_001117"
     CDS             30034..30486
                     /gene="rplI"
                     /locus_tag="ACG80T_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFS4840784.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30760..32127
                     /gene="dnaB"
                     /locus_tag="ACG80T_001118"
     CDS             30760..32127
                     /gene="dnaB"
                     /locus_tag="ACG80T_001118"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFS4840785.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            32907..34199
                     /locus_tag="ACG80T_001119"
     CDS             32907..34199
                     /locus_tag="ACG80T_001119"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFS4840786.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10495 a         6696 c         6815 g        10384 t
ORIGIN      
        1 aacaaataat aatatatcaa catcacatgt gaaagtcaat aagaaaatgc ttatttatca
       61 acatttctag cttacatgat cattggttag ttctggtcct ttttcgttcg tctttataac
      121 tccgagagac gtaaaatcct tttgttttca gtttaatgtt catgttcttg tcttattcac
      181 accaactaga tgtctcacct tctaaaattt tccttaacaa agtgctgctt ttatctattt
      241 ctgctttttt ttgctagaat aatagtaaca atatgactct ggaggattta tttatgaaaa
      301 ttttgattgc tgacgatgat aaagaaatcg ttgaattatt aagtatctat gttcacaatg
      361 aggggtatga agcagtaaag gcttatgatg ggaaagaagc attatctaaa atcagaacaa
      421 tccctgatat tgaattactt attttagata tcatgatgcc tgagatggat gggatgcaag
      481 tagtgaaaga gctacgaaaa gaatcccaaa taccaatcat tatgctgaca gcaaaaacta
      541 ctgatatgga taagatcaaa ggactggtcg ctggtgcaga tgattatgtg accaagccat
      601 ttaatccgtt agaagtaatg gctagagtaa agtctttatt acggcgtaca aaaatgcaga
      661 ttgccccaga tcagcctgat gtattagaag taaatgcatt aatcatcaat aaagactctc
      721 atgaagttaa aacaatcgat gggaaagaaa tccagttaac agctttggaa ttcggtatcc
      781 tctacttgct tgctagtcat cctaatcgag tatttagcgc ggatgaaatt tttgagcgtg
      841 tttggaaaca agaaagtatt gtctctgcta aaactgtcat ggttcacgtt agtcatttaa
      901 gagataagat tgaagaagca actggtggtg aaaaagtcat tcaaaccgtt tggggagtgg
      961 gctataaaat tgacgcaaga tagtaaacga gcaactgaaa aacgccgtcg gatcaatttg
     1021 acttcaaaag aaatcagcga attgttagcg gaaggaatca tcacgattat ccttttgctt
     1081 ctattaaatg tttctattct ggttgttatc agttcggtga tcaacagttc accttctttg
     1141 acgaatgcga tttgggattc aaagaatatt tttgcagaac gattgaacac tgatttgttc
     1201 tggaacggcc ggaattttat tattcccttt ttctttctat tagatattgg tgttttgtat
     1261 tggcgcttga ttcgaagata tcgccaaatg caattgcggc atatcatcag cgaacttcac
     1321 tacatcgcaa acggaaatta tgatcatcgt atcccttttg aactgagtgg tgacttaagc
     1381 cgagtggtaa caagtatcaa tggattggtg gatagtaccg tagcagcgat tgaagacgaa
     1441 cgtaaaatcg aaaaatcaaa agatgaattg atcacgaatg tcagtcatga tatccgtacc
     1501 ccactcacgt ccatcattgg atacctagga ctgatagaag atggccagta tcactcagaa
     1561 gaagatcttt taaagtatac gcatactgct tatatcaaag caaagcagat gaaatcttta
     1621 gtggacgact tatttgaata cactaaggta cgccagcctg ctgtcccggt gaatttttct
     1681 gcctttgaca tgatccagtt gatcgaacag ttagcagctg attttgagtt ggaagcttct
     1741 aaaaaaaata tccaaatact tgttcaatca aaagtagact cccttatcat ggatggcgat
     1801 accgaaaaac ttgtacgtgt cttcaataat cttttgacga atgcgttgaa atacggaaaa
     1861 ggagcaacga agatcgtaat cgaagtagaa cgtatcggtt cagaagttgt tgcaacagtc
     1921 aaaaataacg gagcaatgat cccacaacag gcaatcgata atctattcga ccgtttttat
     1981 cgtgtagaag aatctcgttc acaagcaact ggaggaactg gacttggttt agcaatcgct
     2041 caaagcattg tggctttgca cggcggatac atttatgcaa aatcagataa gcaatggact
     2101 tctttcatca tgcaccttcc aatcaagaaa aatgaaaaat tacctattga tacacaggaa
     2161 gttattgacg aaagctgaaa tattcgctat tcttattaca attattagaa taaatgaaaa
     2221 acacagaacc cagtacactt tattcttttt cagagagctg atggcaggtg taaatcagca
     2281 aaggataatt tggaagttcc agttcgttta cgtgcccttt cgaaataaga aaggtcggtt
     2341 caacagccgt tatgtgttca acaagtgcag ctgacgctga acttgggtgg taccgcgaat
     2401 ttcagactga ttcgtcccaa gaataatggg atggatcagt ctttttttat tttcaaaaag
     2461 gaggaaatca tatgttagat gtaaaaatga tgcgacaaaa tattgaagaa gtacaagaaa
     2521 aattgatgac tcggggcgta aaaaaagaaa cattagctga attcatggaa ctagatgaaa
     2581 atcgtcgtaa gctactagtc aaaagtgaag aattaaaaaa atatcgaaat gatgtttctg
     2641 ctgagatcgc ccaattgaag cgtgcaaaag aagatgcttc tgctaaaatc gctgaaatga
     2701 aagaagtcgg tggcaacatc aaagcattgg atactgaaat tgctgaagtg gatgaaaaat
     2761 tgaaacaaat tgcaactact ttgccaaacc ttccccatga ttctgtgcca gttggaacag
     2821 atgaagagga caatgtagaa atccgtcgtt ggagcgaacc acgtacattt gcttttgaac
     2881 caaaaccaca ctgggaaatt gcagaaaatc ttgatatttt agattttgaa cgtggcgcaa
     2941 aagtagccgg tagccgtttt gtctattaca aaggattagg tgctcgatta gaacgtgctg
     3001 tctacaactt catgcttgat cagcatattt acgaacacgg ctatactgaa atgatcactc
     3061 cttatatcgt caacagtaaa gcgatgtttg gtacgggaca atttccaaaa ttcaaagaag
     3121 atgttttcca attggagaat actgatctaa cattaatccc aacagctgaa gtaccattaa
     3181 ctaactatta taatgatgaa attttagatg gtgcagattt acctatttat ttcactgcac
     3241 taagccctgc ttttcgttct gaagccggaa gtgctggtcg ggatacacga ggactgattc
     3301 gtcttcacca attcaataaa gtagaaatgg tcaaattttc ggatgtagag cattcttacg
     3361 aagaattgga aaaaatgaca gctgatgcag aagatatctt gaaaaaattg catttgcctt
     3421 atcgtgtgat ggccttatca actggagaca tgggcttttc tgcagctaaa acttatgact
     3481 tagaagtatg gatacctgca caagatactt atcgtgaaat cagttcttgt tctaactgtg
     3541 aagatttcca agcacgccgc gcaatgatcc gttatcgcga taatgatgga aaaatccact
     3601 atactcatac tctgaatggt tcaggacttg cagttggccg tacagtagct gccattttgg
     3661 aaaactacca aaatgaagat ggttctgtca cagttccaga agcgctcgtt ccttatatgg
     3721 ggaatctgaa agttattaaa taaagaggct gtgaaaaagc cgcttagttt cgagtattaa
     3781 gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta ataccgagaa
     3841 actggctttt gaacaccatt tatcgagagg ttgtgaaatc agcgaattaa cctgagtatt
     3901 agagaaaaaa atggaaaaat agtccctcat attttaccgt ttgttgaact aataccgagg
     3961 gtcagctgat tgaacaccgt ttagtggaga ggtcgtaagc ctacccccca gctcctagtt
     4021 acaaggacaa attcaaaaaa cagctcttca tattttatga atgttgtact tgtgattcgg
     4081 aggtggcaag ggaacactgt ttattcaaaa agaggctggg acagaagcgt ttaactccaa
     4141 gaaataagaa gaaattcacg aaaattgctt ttcaaatttt tgtgaatttc ttcttatttc
     4201 cgaaggagtt gcttctactt ccgccgttta tacgtgttta gagcgtgaga caaaagtgtt
     4261 ctttactttt gtcccacgct ctttaatgtt tttaagattc aactgaatag tttggtgctt
     4321 cttttgtgat ttgtacatca tgagggtgtg attcttttaa tccactgccg ctcatttgga
     4381 taaattgcgc ttcatcacgt aattgctgta gattggctgc cccaacataa cccattcctg
     4441 aacgcaaacc gccgatcatt tggaaaataa tatctgcaac acttccttta taagcaaccc
     4501 gaccttcgat tccttctggt actaatttat ttgcttcatt gacactacct tggaagtaac
     4561 ggtcttttga acctttttcc atcgcgccta atgagcccat acctcggtat gttttgaatc
     4621 gacgtccttg gaagatttcg aattcacctg gtgattcatc tgtccctgcc aacatacttc
     4681 ctagcatcac tgcatgtccg ccggcagcta atgctttgac gatgtcacca gaatatttga
     4741 tccctccgtc tgcaataatc gctttgccat attgacgagc aactgatgct gcatcataaa
     4801 tagctgtgag ctgaggtaca cctacaccgg ctaccacacg tgtcgtacaa atcgatcctg
     4861 gtccgatacc aacttttacg acatctacac ctgcatcata taaagctttt gtcgcttcag
     4921 ccgtcgcaac gtttcctgca atcaatgttg catctgcaaa agttgaacgg atttcttgaa
     4981 tttttcggat cacacctgca ctatgtccat gagctgtatc gatgatgatt gcatctgccc
     5041 ctgcttctaa taaagcattc gcacgctcaa atgtatcgct tgttacacca acggctgctg
     5101 ctacaagcaa tcgaccatgt tcatcttttg ctgcatttgg aaattcgatc actttctcga
     5161 tgtctttgat cgtgatcaat ccgcttaaac gaccttcgtt atctacgatt ggtaattttt
     5221 cgattttatg tttttgcaaa atcttttctg catcttttag agaggtacct actggggcag
     5281 tcactaagtt gtcttttgtc atcacatcag caatcggcat gtgataatct gttacaaaac
     5341 gcatgtcgcg gtttgtaatt atccctacca attttctgtt ttcaagtgtt tcaacgattg
     5401 gcacaccact gatacgatat ttgctcatca attcttctgc gtctgctaca agatgctgtg
     5461 gcgttaagaa gaacggatca atgattacgc cactttcaga acgtttgact tttcttactt
     5521 catccgcttg ctggctgatc gtcatgtttt tatggatcac acctagtccg ccttgtcttg
     5581 ccatagcaat ggccatcttg ctgtcggtaa cagtatccat acttgcactg atgattggaa
     5641 tattcaactt aatattttta gctaattgta cgctcatatc cacttcgttt ggtaacacgt
     5701 gactttctgc tggtatcaat aagacatcat caaaagtaaa tccttttttc gtgaactttg
     5761 tttcccagtt ggacattttt agtaccactc ctctaattta gtttatctat taaaataatt
     5821 gattttttca tttaagtcaa tgatctactt ggaatcaagt tagctttctc atagaatacc
     5881 aatataaagc gtttacatct gtgtgtatta aaagagattg taaaaaagct gtcctgcatc
     5941 aagtaataag aacagccaat caaaaacagc ttctcatgtt tttgaattgg ttgggtttat
     6001 taccgcagat gcaaactttt gaacaccgtt tagtggagag gctgtgaaaa agctgcttag
     6061 tttcgagtag taagaaaaat tttgtgaaaa tggctttcca catttttgca taaattggac
     6121 ttaataccga gaaactggct tttgaacacc gtttatcgta aaaagggact gtgacaagac
     6181 ttttgtcaca gtcccttact attatttaac tatggataaa caaaatgagg ttctttacta
     6241 cccctagata tctagcaaaa tagctgatct ttttgctagt ttatcttctt gggacggata
     6301 aacttccaat aatcccgtat tttttcttca agtagtaacg gattgctaaa gcagttgccc
     6361 cgataatgat aactactact ggatctaata ctggattgat aactggtggc aatagtgttg
     6421 tcgcagaaaa cagtacgacc caaagaagtg tcgttccacc taggataagc aatgtcttac
     6481 ccattcctgg tttcttgctt cgatctgctc cgggatattc atattgatag atgtatttgt
     6541 acatcatata aaagacccag ccaccgattg cagaacctag aacgagtgta gaaaagccat
     6601 aagctggcgt tgttccacgt gaaaacaatc cggtaatcgc aatcatgata cttaatagac
     6661 caagcaacag caacgtatta tctaaccaca tcaataaagg ggtggatgaa ttttttactt
     6721 ctgctggctt attgatgatt gcttccgttc tttcagagac tgtcccaaac aattgacggg
     6781 ctgttcttcc gcctttttgt tctgcaacta aagtaggcag aatgtcatga agggctttta
     6841 ctttttcttc ttcagacaaa ttcgctgctt ctaaagattt tttcaaatca aaaatgtact
     6901 gttgatttct tttggttaat tttgtttcta aactattgtt ttcttttacg attgctcgta
     6961 attgttctgg ttccattatc taatagtcct ccttagccgt tccttttctc aacggtcgtc
     7021 acaaaagaca ttctaccata ttctacagct agtttgatag cccttttaca cattaaagcg
     7081 gaataacatt acgtcgccgt cttgtacgac ataatctttt ccttctaatc gaacacgtcc
     7141 agcttctttt gctgcatgca tgtttccgta atgatccaag tcttcaaaag agacagtttc
     7201 tgcacggata aatccgcgtt cgaaatctga atggatgatt cctgctgctt gtggtgcttt
     7261 gattcctttt ttgaatgtcc aagcacgtac ttcttgttcc ccagcagtaa agtaagtagc
     7321 taagcctaac aaatcataag cagcacggat caactgatcc aaacctgatt cttcgattcc
     7381 taaagcttct aaaaattccg ctttgtcttc ttcatctaat tctgcgattt cttcttctgc
     7441 acgagcactg accacgatca cttctgcatt ttcttctgca gcaaagttgc gcacttcttg
     7501 tacataatga ttcgcgtctg catctgctac gtcatcttct gagacattgg ctacataaag
     7561 aattggtttt gtcgtcaata aaaatagtga tttaacgatt ttttgttctt cttcagtaaa
     7621 ttcgatcgtt cgggcagatt ttccttcttc cagtacaggt ttgattttat ccagtacagc
     7681 tagttctgcc actgcatctt tatctttggt tttagcaact tttgctacac gagtatagcg
     7741 tttgttgata gattctaagt ctgctaacac tagttccaag ttgatcgtat caatatcttc
     7801 tagtggatca acacgtcctt ctacgtgagt aatattatca tcatcaaaac agcgaacaac
     7861 gtgacaaata gcgtctactt ggcggatatg gcttaagaat tgatttccca aaccttctcc
     7921 tttacttgcg ccttttacga ttccagcaat atctgtaaat tcaaaagtcg tcggaacagt
     7981 ttttttcgga tgtacaagtt ctgttaagcg ttgcaaacgc cagtcaggta cttctaccat
     8041 ccctacgttg gggtcgatcg tcgcaaaggg atagtttgct gcttctgctc ctgcttttgt
     8101 gatcgcatta aacaaagttg attttcctac gttgggcaac ccaacaattc cagcagtcaa
     8161 tgccataaat ttattcactc tttctctgca tttttttcaa ttactttttt taatttcttt
     8221 tcaaaatctc gacgggtcat catgacgata tgtccgcaat tcatacatct gattttgata
     8281 tctgcgccca ttcgaatgac ttcccagcga ttcgtctggc aagcgtgagg ttttttcatt
     8341 tcaacgatat cgtgcaagtc gtacataaaa atagcctcct ttttgtttat tcttcatcaa
     8401 attgaatatt caagatatca agaattcgag tcaaatcttt ttgagaaaga tattcaatct
     8461 cgatcttacc tttcccattt ttttcttgga tagctacact tgtaccaaat ttatccatca
     8521 aacgatcttc gctttctcgg atataatatg gtttttcctt tgctagcctt gggatttttt
     8581 tcttttcttt tccatcattt tgatttaact cagaaacaag ctgttccagt tggcggacag
     8641 ttaaattttc agttactgca cgattggcta atttcaagat caatgtttta ttttttaaac
     8701 ctaacagcgt acgggcttgt cccatagata aacgctgatc ttgtaccatt tccttgacaa
     8761 gatccggtaa agaaagcaag cgcaaataat tggcgatata aggtctgctc tttcctaaac
     8821 gactagctac ttctgcttgt gttagtttca ggtttttcat cagcatctca tatgcttctg
     8881 cttcttctaa cggattcaga tcttctcgct gcaagttttc cagcactgct acttgcatca
     8941 tcgcttcttc gtcaaagtct ctaacgatag cagggatcgt ttctttttca gctagtttgg
     9001 acgctcgaaa acgtcgctcc cctgcaataa tctcatatcc ttttactgcg gaacggcgta
     9061 cgatgatggg ttgaaagaca cctgattgtt caatagaatt agctagttcc tgcaaagata
     9121 cttcttcaaa tgttttacgg ggctgatacg gatttggtcg cagctcattc aatggtagct
     9181 ggacaacggt atcatctttt acatccaccg tctctaagtt ttctaaatct tgaaacagtg
     9241 catcgattcc tcggcctaat cctttacttt tcttcacgac ttaccacttc ctttgccaat
     9301 gcttggtaga cttcagctcc tcgagaacgt ggatcgtagt cgatgatcgg ttttccatga
     9361 ctaggtgctt ctgataaacg aatatttctt ggaatgatcg tttcgtaaac tttttcgcgg
     9421 aagtatttac gtacttcttc tacgacttca tttcccagat ttgttcgtgc atcatacatt
     9481 gtcagtaaga ctccctcgat ctctaattct ggattaaagt gtttttggac taaacgaacc
     9541 gtattcagta attggcttaa tccttccaat gcgtaatact cacactgaac aggtatcaaa
     9601 atcgaatcac ttgctgtaaa tgaattgatt gtcagatggc caagagaagg cgggcaatcg
     9661 atcaaaatat aatcatactg actgcttact tctgctaaag atccttttaa tcttgattct
     9721 cttgccatca ttgacgtcaa ttcgatctct gctcctgcta attggagagt cgcaggcaca
     9781 atactcaaat tttcgtgttc agtgatcaaa gttgcctcat caattggcaa ttcattgact
     9841 agaacatcat agatgtctct tgtcacatca ggttttctga tcccgacacc actggtagca
     9901 tttccttgtg catccatatc cactagcaat acttttttgc caaggctagc aagacaagca
     9961 cctagattca ccgtcgtcgt tgttttgccg acgccacctt tttggttcgc aacagatatt
    10021 atttgtgcca tctcttctcc tcctatttga ttggctgctt attaggcagt ccgggctttc
    10081 ttgggtattt ctttggggtt ggtttctttt tttgaataac gatgatatgc cgttgatcag
    10141 agacattcgg caaggtaacc gcctcttctt tgaggaattt accacctaat aaagcaatcg
    10201 cttgttttgc ttctgtcaat tcctcttcac tttttgctgc tttcaatgca taaaaatagc
    10261 cttcttgttt agccaagggc aaacacaatt ccgctaacac attcaaacga gcaaccgcac
    10321 gagcagtgac aaggtcaaat gatgcacgaa agtgaggatt ttgcccaaat gtttctgccc
    10381 ggtcatgata taaggaagca tcgattccta ataatcgggt taactcagat aagaaggtta
    10441 ttcgtttatt cagagaatca acgatcgtta tctgcagttc tggaaaggca atttttaaag
    10501 gtatgctagg aaaccctgcc cccgaaccaa catcgcataa agataaaggt tgcgtaaagt
    10561 ctacttcaaa tgctaatgta agtgaatcgt aaaaatgctt cagatatacg tctttttcat
    10621 ccgtaatagc tgtaaggttc atcttttcgt tccattcgac taatgtccga taataaatag
    10681 cgaattgatt catttgatga tcggtaagct ctatcccttt ttctgccaag gattggcgaa
    10741 attcttctgg tatcataaat attcctttca ttgtgaaaca gcaattgttt cacagattct
    10801 ctccctactt taacgcaaaa tctctagaca tgcaatcctt ccatatggat tcttaaaaat
    10861 ttgaaaagta agaaaagccg atagactttt tctttctagt atgactcata aaatatacac
    10921 agaagagaga actagctttg agcaatttct gcaaaaacta gttctctctt ctatttatta
    10981 ttacgtatta cgctggttca ctgactttcg caatcttccc ttgttcgata tagaccatta
    11041 ggatacttag gtctgcaggg tttacgccac tgattcggct agcttgggca attgtttctg
    11101 gttggatttt ctgcaatttt tgttttgctt ctgttgctaa tccgttgatt gctgcgtagt
    11161 cgatgttttc tggaatcttt ttcgcttcca tgcgttttaa tttttctact ttttccatgg
    11221 cttttttgat gtagccttca tatttgattt ggatttctac ttgttcgatt tcttttcgtg
    11281 tcaattcttc attttctgga ataaaccgta gaagttctgc ataagtaatc tcaggacgtc
    11341 ttaaaaagtc gcttgctagt acaccatctt ttaatggtgc cgcattaatc gttgctaaaa
    11401 agtcttggac ttcttctgtt ggtttgattc gtacattcat caaacgtttg atttcttgtt
    11461 caactgcttc ttttttcttc aaataattct tgtattgatc ttcttttact aagcctacac
    11521 ggtgacccat ttctgttaat cgcaaatctg cattatcatg acgtaaaagc agacggtatt
    11581 cagcacgtga agtcagcaaa cgataaggtt cgttcgttcc ttttgttacc aaatcatcga
    11641 tcatgacacc gatgtaaccg tcacttcgtt tgatcactaa aggttctttt ccctgaactt
    11701 tcaaagctgc attgataccg gcaatcagcc cttgtccagc cgcttcttca taaccgcttg
    11761 taccatttgt ttgtccagct gtataaagat tttcaacgac tttagtttct aatgtcggac
    11821 ggagctggtg tggctctact acgtcatatt cgatagcata tcctgtacgc atcatttcta
    11881 ctttttctaa tccagcaata gaatgaagca tgttgacttg aacatcctct ggtaaagaag
    11941 tagataaacc ttgaacatag acttcttccg tatttaagcc ttctggttcc aaaaataatt
    12001 gatgacgtgg tttgtctgca aaacgcacga tcttatcttc gatggatgga caatagcgag
    12061 cgcctacccc ttcaacgatc cctgtaaaca ttggggcacg atgtaagttg tctcgaataa
    12121 tttggtgagt cgtctcacct gtataggtca gccagcaaga ttcctgatct agtttatagg
    12181 cactatctgg tgtgctgaag ctaaaatgat tcggctcttt gtcaccaggc tgctcttctg
    12241 taacagaata gtcgatcgta cttgatttta ctcgaggcgg tgttcctgtt ttgaaccgtt
    12301 cgatagcaaa gccaagttct tttagatgat tcgctagtcc aatagaaggt tgagagttat
    12361 tcggacctga tgaatatttc aattcaccga taatgatttc tccgcgaagt gctgttccgg
    12421 ctgtaataac aactgctttg gcccggtagc tcgctcctgt ggacgtcact actccatgac
    12481 aaacaccatc ttcgacgatc agttcttcga cgattccttg tcttagcgtc aaatttggtt
    12541 ctttttcgat cgtatgtttc atttctgttg aataagcatg tttgtctgct tgtgctcgca
    12601 atgctcggac agctgggcct tttcctgtgt tcaacatacg catttggata taggttttat
    12661 cgatattctt acccatttct ccgcctaacg catcgatttc tcgaacgaca acgcctttag
    12721 ctggtccgcc tacagaagga ttacatggca tgaatgctac catgtccaaa tttattgtca
    12781 gtaataatgt ttttgatccc atacgtgcag cagcaagtgc agcttctgaa ccggcgtgtc
    12841 ctgcgccaac aacgatcacg tcatacgcat ctgcttcata atgttccaat cgtttgttcc
    12901 tccttttatt ttcctaaaca aaattgactg aataattgag tgattagctc atcttgtaca
    12961 ctgtcgccga caacttctcc tagatagtcc cagcaacgcg tcatatctat ttgaacgaga
    13021 tccacaggca ttcctgaatc aatacctgca atgacttctt caagtgagag ggaagctttt
    13081 tctaacaacg cgatatggcg ggtattagaa acataagtag cgtctctttc tcctgtttct
    13141 ccgctgaaga ataaatcaga aatagctgat tccaaacgat ctagtccgtc gtttttagct
    13201 acggaaattg aaaaaactgg ttcattttcg atcaatttct tcaatttttc ttgttccagc
    13261 tgtgctggca aatctgtttt atttagaaga atgatccgtt tcaatccact tgtcgcttcc
    13321 aataattgtt catcttctgc tgtcagtggt tcgctttggt ttagaacaag taagatcaag
    13381 tctgcttctg ctaatgcttt tcggcttctt tcgaccccga tccgttccac cacatcttct
    13441 gtttcccgaa ttccagctgt atcgattagc ttgagcggta ccccccgaac attgacatac
    13501 tcttcgatta catctcgagt cgttcctgca atatccgtaa cgattgcttt ttcttctctt
    13561 aacaaatggt ttaacaaact tgatttgccg acatttggcc ggccgatgat tgccgtactt
    13621 aacccttccc gcagaacctt cccttgcttg gatgtagcaa gtagcgcttg gattctttgt
    13681 tggatcatct gcgctttttc aagcaataac ttcgtagtca attcttcgac atcatcatat
    13741 tctggataat caatatttac ttctacttgt gctaatgtct ctaaaatctc ttgtctcaat
    13801 gagcggatca aagcagacag attgccgtct aactgattaa gagctagtcc cattgctttg
    13861 tctgtttttg cccgaatcaa atccatcaca gcttctgcct gtgaaaggtc aacacgacca
    13921 ttcaagaatg ctcgttttgt gaattcgcct ggctcagcta atcttgcacc ttctcttaac
    13981 aatagctgca aaatttgatt gactacaacg atcccaccgt gacaattgat ttctactaca
    14041 tcttcccgcg taaatgtctt tggtgctctc atcacggaga ccatgacttc atcgacaagc
    14101 tgattgctct tgggatcaac gatatgtcca taatggatcg tatgacttgg tacttctgat
    14161 aatcgtttgt ttccagattg ataaacttta tctgctagtt gcacagcttg atcgccactt
    14221 aaacgaacga tactgattgc accttctcca ggtggggtag aaattgccgc aatcgtatca
    14281 aattctaaag taatttcagc cattttttca cttccttttt cattttgcgc acaaaaaaag
    14341 tgcccactcc accctttatg cacaataaac ggtgaacttc gcactttgac tgcttgttct
    14401 tgattaaatt ctggtcatag attatcacac ttgctataag gaatcaagtg tttaaaacaa
    14461 ttagatgaag ttgagcctgt tctgcttcca ttaccttata tttaattgtc cttggatgtc
    14521 tctttgcgtt ctttggttta ttcgaagaga agatactgta taaacaaggc tttatctttc
    14581 ttaaaaaaaa cggtacacta aatggtagcg ctatctaaac tatacacttt tgaaaggaga
    14641 aaatatgtga gcccgttcag atctgctgca ttggaagaag aaagtgaccc tcggatcaac
    14701 gatgtcgttt tatctacagg gaatatcaac cagaaatata tcgtccgcga tatcgttttt
    14761 gttgtggaat gcttagaagc tgatttgttt gacccatctt ttcccaaaga tgaccttttc
    14821 ttaacaacga agaggaaatt aaagacgaag gtacttgaat atggtgcgaa tgcagtaatc
    14881 aactgtcatt ttgagtatca gcaaaaaaaa cgcgacggaa agtattttct ggaacttttt
    14941 gcttatggaa cagtgatcca atttatccaa acaacgattg gttaaatgaa acagacaaaa
    15001 aaagcggatc aaaactgaag acagtttcga tccgcttttt tctatcaaag ttagtgacta
    15061 cttcatcgta tactctgata agaatgattt ctagtaaaat atacttggtt tgaaaaactt
    15121 tgtcaacgtg atttattcaa aaagtaatgg aatcaataat attttttagc aggttctact
    15181 actaaataac gataaggttc ctcaccttcc gaatgtgttt ttacatgttc atctttgctt
    15241 aatgcagcat ggatttgttt acgttcaaat gcaggcattg gttctaagaa aacaggctgc
    15301 cctgtccgtt ttactttttc agccgtgcga tcagctaaac gctgaatgat ctcttggcgc
    15361 ttttcacggt aattgcctac attgactact acagacagct tatttcttgc taaacgatgg
    15421 atgaagactt gtgccagata ttgcaatgca ttcagtgttt ttccatgttt cccaatcagt
    15481 atgccttgct tttgtgtttc taaatggaaa acaaccatat tatttgattc tctttccatt
    15541 ttgacaagag ctggagcatc aagctgtttt gaaatttcag taagataaac tgccaattcc
    15601 gttaaagctg cttcatcacc tagctcactt acttcttctg ttttttcttc aagaatcgtt
    15661 tcgttgacta cctcgatctc agctgttggt tcttcttcca aaatttcttc tacagtctgt
    15721 tctactgttt cagaaacttt ttcgctgata cttggctgga tcgaaagccg tgcattcttt
    15781 cgacccattc ctaggaaacc tttttttcct tcttccagta tttcgatttc gacttggtct
    15841 ttggccaaac ccaaatcagc cagacctagt tggacagctt cgtctattgt tgcagcttca
    15901 taaatcggca ttagcattac ctcccctatt atttccgttt tttcttagga ttcattgctt
    15961 tcttaagagc acgttctctt tcgcggattc tccgtgcttc ttcttcacgc tcttttcgaa
    16021 ttttgaaagg gttgttgatc agcagcgttt gtacaacttg gaaggcattg gaaactaccc
    16081 agtataatga aagaccgctg gctaaattga cacccatcac taaaatcatt acaggcatca
    16141 cgaaattcat gatcttcatt gatgcattcg attcaatttg actcatgcta gataaatacg
    16201 tacttgcaaa agtaaagatt gctgctaaaa tcggcaaaat aaatgttgga tccgcgttcc
    16261 ctaagttcaa ccataagaaa tgcccttgtt tcaactctgg tacacgtgaa atagattgcc
    16321 ataacgccat caagattggc atttgtacta ataaaggaag acaaccagca tatggattga
    16381 cgccattttc tgcatataaa cgctgctgct cttctcttaa cttactttgt gtttccggat
    16441 ctcttgaact atattgttgc tgcagtgctt ttaatttcgg ttgtaattct tgcgttttac
    16501 gcatgctctt tgtttggaaa tgcatcaacg gcaataggat cacgcggatg atcagtgtaa
    16561 ataaaatgat ccctatccct gcattaccaa atgacaacgc cttgatcgcc tcagcaaagt
    16621 aataaacaat ataacgatcc caaattcctg tactctgtgc gctgacttcc ccagttccgc
    16681 aaccggataa gacgatgacc aaactaacga taccggccat caaaagtaaa cgtttgtatt
    16741 tcttcactta ctcttttcct ctctgtcgat tatttttgca agttttaaga cgtggactaa
    16801 attggagcga acctcatcat aagtcaaccc cttcacactt ggtctagcaa tcacaataaa
    16861 atccaactcc ggatcaattt cttcttttat agaataaact gctgctcgga tttttcgttt
    16921 gacttcattg cggtttaccg cgttgccgac cttttttcct acagataaac caactcgaaa
    16981 atgaggctgt cccggttttt ccaaacggta aacgacgaat ttgcggttag caaaagaatc
    17041 tcccgtatgg aacacggctt gaaactctcg ttcttttttt actcgatacg cttttctcat
    17101 tagttttctt tccttttcta tctctcactt acgctcataa tcataccata attacgattg
    17161 tcctttcatc atttccctaa aaaagagtat aaaaaaaacc actgaaaccc cagtggccta
    17221 agctgaaaga acttttcttc ctttgcgacg acggctagct aacacacgac gtccattttt
    17281 agtactcata cgtttacgga atccgtgaac tttttgacgt ttacgtttat ttggttggta
    17341 tgttcttttc atatattcca cctccaaaat gttcttatct attttataaa cacagacata
    17401 ctttgatagt ataacgacaa agtagtgatt ttgtcaatat aaaatagaag atcgattgtt
    17461 aaaacttatc cacaagatta tttttttcta tgggaatcct atgtggaaaa agttatgcac
    17521 agggattatc cacagataat aaaaggctgg ttttacacag aaaacacacg tgtaaaaaaa
    17581 gtaatccaca atcaggcgat ttcctgtgta ttccgtctcc taactattgg tatatctgta
    17641 ttttttatcc acagacgcaa gaggaaatct attggttttt acgagttttc cacctgtgca
    17701 ttagtggtgt ggaaaacttt ttttgacaac tgtgtttttc tcttttttct ttttgtcgat
    17761 tgtgtggata attgtaatta aaaaattttt ttcttcggtt tctgtggaaa actattgaaa
    17821 aaaatgctaa attagtacta gttattcact ttttaatgaa ggaggaggcc ttatatggta
    17881 tccctcgatg ctttatggaa tgaattaaaa gcaacatacc aaaaggattt atcgcctgct
    17941 agctataata catggatcga aacagcgaac cctcgaaccc ttgatcaaaa ccagctcgtt
    18001 gttgaggttc ctagcaagat tcataaagaa tattgggaaa aaaacctggc gacaaaaatc
    18061 gttgaagtcg gttatatgtt atcaggaaat gagattatcc ctcgctttat tactggcgaa
    18121 gaagcagagc aagaagaagt tatagaagaa aaaaatccaa aagttgtggc acctagtccg
    18181 ctaaaaaaag ccatgctgaa ccctaaatat acctttgata cgtttgtcat tggtaaagga
    18241 aaccagatgg ctcatgcagc agcgctcgtt gtagcagaag atccaggttc tatttataac
    18301 ccgctcttct tttatggagg cgtgggactt ggtaagactc acttgatgca tgcaattggt
    18361 catcaaatgc tgcaaagtca gcctaatgcc aaagtaaaat atgtaagcag tgaaactttt
    18421 gctaatgatt tcatcaattc gatccaaaac aaaacagcag aagagttccg tcaagaatat
    18481 cgaaatgttg atttactgct agtggatgac attcaatttt ttgcagaaaa agaagctacg
    18541 caagaagagt ttttccatac tttcaatgcc ttgtacaatg aagggaaaca aatcgtacta
    18601 accagtgatc ggttgccaaa cgagattccg aaactgcaag aacgccttgt ttctcgcttt
    18661 gcatgggggc tgtctgttga tattacaccg ccggatcttg aaacaagaac agcgatttta
    18721 cgtaaaaaag cggcggctga acgtctagag atcccagatg acacactaag ctacatcgca
    18781 ggacaaattg attctaacat tcgtgaatta gaaggtgcgc ttgttcgagt gcaagctttt
    18841 gctgcgatga atagtgaaga tatttcgact agtctagctg cagacgcact gaaaacgtta
    18901 aaatcaggaa aaggacatcc tcaattgtca atcctgcaga tccaagaaga agtggcaaag
    18961 tattatcaca tccaattgaa agatctcaaa ggaaagaaac gcgtcaaatc tattgttgtt
    19021 cctagacaaa tcgctatgta tttggcacgt gagctcacag ataattcttt accgaaaatc
    19081 ggagctgaat tcggtggcaa ggatcataca acggttatcc atgctcatga aaaaatccag
    19141 cagctgatgg attctagtgt cagtatgcaa aatgaagtat cagaaatcaa aaacttttta
    19201 ctaaattaat ttgtcgattc tatcaactga aaatagcatg tggataaaag aaaaaaacgt
    19261 tcaaaagtta tacacacttt ttccacagct gttttttcgt ttctctctta cgttttttga
    19321 gttttccaca gaatcaacat gccctattac ttttattatt ctttttaact aataataaat
    19381 aaacaaacat tcaaaggaga tacatcatga aagttacttt aaaccgagct agctttatgc
    19441 aggaattgca aactgttcaa cgagctattt caagcaaaac cacgatccct attttgacag
    19501 gtgtaaaaat cacactgaca caagaaggtt tgactttgac ggggagcaac gctgatatat
    19561 caattgaaac ttttttgtct gttgaaaacg aaaaagcaaa tatgcaaatc gaatctactg
    19621 gttccattgt tttacaagca cgtttcttta gcgaaatcat tcggagactt cctgaagaaa
    19681 catttacttt agaagtttta gaaaataaac aagtagcgat cacttctgga aaagcgaatt
    19741 ttatcgtaaa tggattagat gcagataact atcctcatct tcctgttgtc gaaagccata
    19801 accagatgaa attacctgta cacgtattga ctaaactaat caacgaaaca gtttttgctg
    19861 tctcacaaca tgagagtcgt ccaatcttga caggtgtgca tttcatttta tctgataatt
    19921 ctttattagc tgtagctact gattctcacc gtctaagtca acgcgtgatt ccagtagaac
    19981 aagcggctga tcattttgat attgttattc ctggaaaaag tttgatcgaa ttatctcgct
    20041 cattaacaaa tgaagaagaa atcgtcgaaa tcagcattat ggaaaaccaa gtgttgttca
    20101 aaacagaaac gatgtatttc tattcccgtt tgctagaagg aaactatcca gataccaatc
    20161 gtttgattcc ttcaagtttt aatacagagg ttgaattttc tgttcctagc tttttagcgg
    20221 cgatcgaacg ggcttcttta ctttctcatg aaggccgtaa caacatcgtt cgtttatcta
    20281 ttcgtccaga tgcggttgtt ttatacggaa attcgccaga aatcgggaaa gtagaagaaa
    20341 gcttgagtta tacagcaagc agtggcgatc cattagatat ttctttcaat ccggattata
    20401 tgaaagcagc gttgcgtgca tttggagata tgagcatcaa agtgaaattt atctctgcta
    20461 ttcgtccatt tacattggaa ccaacggaag atggcgttca atttattcag ctgattactc
    20521 ctgtacgtac aaattaacaa tgattttcaa tgttcttgaa aatttaattg aaaagaaata
    20581 gaaaaaataa aattttaaac agaaggccct aaaatctctt ttaaggcttt ctgtttattt
    20641 ttgtttaaat cacctttttc agattaaatc gtcttatatg gaaaaatata tggataaatt
    20701 tgttttattg cgaaaaaaaa ggtataatag agtatacttt taataatgaa aatgagggat
    20761 gggcattgaa acaacaagtc attctaaata cggaattcat gacgttgggc caaatgttga
    20821 aagaagtcac agttatcggt agtggcggac aagcaaaatg gtatttggca gaaaacactg
    20881 tgttagtcga tggcgaacca gaaaatcgtc gtggacgcaa actttatccc ggcatgatgg
    20941 ttgaagttcc agaagtagga acttttttta tggtaaaaaa aggaaacgac gataatgagg
    21001 ctgaataagc tgtatttaaa aaattatcga aattacgaag agctgaacat cgaattttcc
    21061 aagaacctcg tcatcttttt aggagaaaat gcgcaaggaa aaacgaatct tttggaaagt
    21121 atctacgtat tagctatgac gcgaagtcat cggacaagta gtgagcagga attgattggc
    21181 tggactgacg atcaggcgat gattcaagga gagatcacaa aaggttcatc cactcttcca
    21241 ttagaaatct tattatctaa aaaaggccga aaaaccaaga tcaaccatat cgaacagaaa
    21301 aagttaagta cttacgtggg acaaatgaat gtcattttat ttgcaccaga agatctttct
    21361 ttagtaaaag gcagcccgca gatcaggcgg aagtttttag atatggaatt aggccagatc
    21421 aatccaatct atctttatga tcttgtccaa taccaagcaa ttttgaaaca aagaaaccag
    21481 tacttgaagc aattgaatga aaaaaaacaa actgatttgc tttatttgga tattttgtcc
    21541 gaacaactag cagcgtttgg cagcaaagta ttgaaagcaa gagcacagtt cgtcaagcga
    21601 ttggagtttt gggctaattc tttgcatcag caaatcaccc atcaaaaaga gcagctggaa
    21661 atcgagtatc tcaccgcagt cgattcttta gaaacacata cgcaagagca aatccaagaa
    21721 caatttctcg cacttttgaa ccaaaataaa aagaaagacc tttttcgggg aacaacgaca
    21781 gttggtcctc atcgtgacga tctttccttt tttattaatc agaaaaatgt gcagacttat
    21841 ggatcgcaag gacagcaaag aacaactgca ctaagtgtca aattagcaga aatcgatttg
    21901 attaaagaag agacagggga atatccaatc cttttgttag atgatgtgat gagcgagctg
    21961 gatgataatc gtcaactcca tctgctagaa acaatcgaag gaaaagttca aacgttctta
    22021 acaacaacga cacttgacca tgtaaaaaat aaaatgactg ttgaacctga gattttttat
    22081 gttcaacaag ggaaactaaa aggagaagaa actgaatgac agaagaaaga agtttagtag
    22141 aacgcgctaa agagtatgat gccagtcaga ttcaagtact tgaaggactt gaagccgttc
    22201 gtaaacgacc tggcatgtat atcggttcga ctagttctga gggtctgcat caccttgttt
    22261 gggaaattgt cgataattcg atcgatgaag tattagcagg ttttgcaacg aaaatccatg
    22321 tgattattga aaaagacaac agtatcaccg ttattgacga cggtcggggg atacctgtag
    22381 atatccaggc aaaaactgga cgtcctgcag ttgaaacggt ctttactgtt ttacatgctg
    22441 gaggaaaatt cggcggtggt gggtacaaag tttctggtgg acttcacgga gtaggatctt
    22501 ctgttgtaaa cgcgctttct acacagcttg atgtcaaagt atacaaagaa ggaaatgtgt
    22561 actatcaaga atatcgtcgt ggagctgtag tggacgactt aaagattatc gaacaaacgg
    22621 atcgccatgg aacaacggtt cactttacgc cagaccctga aatttttaca gagacaactg
    22681 agtttgattt ttcaaaattg gctacccgga tccgtgaact tgctttcttg aaccgcggga
    22741 tgcgtatatc gattgaagac aaacgagaag aagaaccagt catcaatgaa taccattatg
    22801 acggcgggat caaaagttat gtcgaatact tgaatgcaaa caaaaccgtg atttttccag
    22861 aacctgtata tttggaaggt gaacagcaag atatcgcagt tgaagtctct atgcaatata
    22921 cagacggcta ccattccaat atcatgagtt ttgccaacaa tatccatacg tatgaaggtg
    22981 gaacacatga atcaggattc aagactgctt taactcgtgt gataaatgat tatgcccgaa
    23041 aacaaaagct gatgaaagaa aatgacgaca acttgaccgg tgaagacgtg cgagaaggat
    23101 tgacagctgt tatttctatc aagcatcctg accctcaatt cgaaggacaa acaaaaacaa
    23161 aattgggcaa ttccgaagta cggacagtca ctgatcggtt atttagcgaa catttcatga
    23221 aattcttgct ggaaaaccca agtgtgggcc ggcaaattgt tgaaaaagga ttactagctt
    23281 ccagagctcg tctagcagcg aaacgtgcgc gtgaagtaac gagacgtaaa ggtgctttag
    23341 agattagtaa tctgccagga aaattagcgg actgctctag caacgatcct gaaaaatgcg
    23401 aattgttcat cgtcgaagga gattcggccg gcggatcagc taagcaaggt cgtaatcgag
    23461 agtttcaagc catcttgcct attcgtggga aaatcttgaa tgttgaaaaa gcaagcatgg
    23521 acaagatctt ggcaaatgaa gagatccggt ctttatttac tgctatgggt acagggtttg
    23581 gagaagattt tgatgtatcg aaagctcgct accacaaatt agttatcatg actgatgccg
    23641 atgtcgacgg cgcgcatatc cgtacattgc tgctgactct tttttatcgt tacatgcgtc
    23701 ctattgttga agctggatac gtctatattg ctcagccacc tttatacggg gtaaaacaag
    23761 ggaagaatat tacgtatgta caaccaggaa aaaatgcaga agaagagttg aaacaagtcg
    23821 ttgcttcact tcctgcctca ccaaaaccaa gcgttcagcg ttataaagga cttggagaaa
    23881 tggatgatca tcaactatgg gaaacaacta tggacccaag caatcggatg atggcccgcg
    23941 tcagtgtgga cgatgctatt gcagcagatc agatatttga aatgttgatg ggggatcgag
    24001 tagaaccccg tcgtgcattc atcgaggaaa atgcacacta tgtgaagaac ttagatattt
    24061 aatcatgaag gaggaaaaag aatgagtgaa gaaatcagag aaaacatcca tgatgtcaat
    24121 ctaaccagcg aaatgaaaga ctccttcatc gattatgcga tgagtgttat tgtcgcacgt
    24181 gcacttcctg atgtcagaga tggtctgaaa cccgttcatc gccgtatttt atacggcatg
    24241 aacgaattag gtgtaacacc ggataaagcc cataaaaaat ctgccagaat cgtcggagat
    24301 gtcatgggta aataccatcc tcatggagac attgcgatct atgagtcaat ggtacggatg
    24361 gcacagccat tcagctaccg ttatatgcta gtggatggtc atggaaactt tggttctgtt
    24421 gacggtgacg gtgccgccgc tatgcgttat accgaagcac ggatgagcaa aatcgctaca
    24481 gaaatgctgc gtgatatcaa taagaacact gttgatttcc aaagcaacta tgatgataca
    24541 gaaaaagaac cagttgtact acctgctcgc ttcccgaatc ttttagtgaa cggaacaacg
    24601 gggatcgccg taggtatggc cacgaatatc ccacctcata acttgtctga ggtagtagcg
    24661 gctatcgatt tgctgatgga taatcctgat gtgacgacca atgaattgat ggaagtatta
    24721 cctggaccag attttccaac aggtggtttg gttatgggga aatcaggaat ccgaagagcg
    24781 tatgaaacag gtaagggttc tattaccgta cgtgcaaaag ttgagattac tgagatgcca
    24841 aatggtaaag aacggatcat cgtgacagaa ttgccttata tggtcaataa agcaaaattg
    24901 atcgaacgga tttctgaact gcacagagac aagcgtatcg aagggatcac agatttgcgg
    24961 gacgaatctt cccgtgaagg tatgcggatc gtcattgaca tccgtcgtga cgccagcgca
    25021 tccgttatct tgaataactt atataaattg acatccttgc aaaattcatt tggattcaat
    25081 atgttggcga tcgaaaaagg tgtgccgaaa gttttgagct tgaaacaaat ccttgaaaac
    25141 tatatcgaac atcaacgcga agtcatcaca cgacggacgg tctttgaaaa agaaaaagca
    25201 gaagcaagag cacacatctt agaaggtttg cgtattgctt tggatcatat cgatgaaatc
    25261 attgcgatta tccgcgggtc taaatcagat gacgaggcaa aagcaacaat gatcgaacgg
    25321 ttcgatttat ccgatcgcca gtctcaagct attttagata tgcgtttacg ccgattgacc
    25381 ggtttggaac gggaaaaaat cgaaaatgaa taccaagaac tcttaaaact gattgaagat
    25441 ttaactgaca ttctggcaag accagaacgt gttacagaaa tcatcaagaa tgaattagca
    25501 gacttgaatc aacgatttgg tgataaacga cgcacagaat tactagtcgg tgaggtctta
    25561 agtctggaag atgaagattt aatcgaagaa gaagaaatcg ttattacatt gacaaacaat
    25621 ggttacatca aacgtttggc aaataatgaa ttcagagctc aacgaagggg tggacgtggt
    25681 gtccaaggaa tgggtatcca caatgacgac ttcgtcaaaa atctggtttc ttgttctacc
    25741 catgatacat tgttattctt tacgaataac ggaaaagtgt atcgggcaaa aggatacgaa
    25801 attccagaat acgggcgtac agccaaagga atcccaatca tcaatttact aggtatcgat
    25861 tcgagtgaaa gcatccaggc aatcattgcg gtgacgggag aagcagaaga aggccactat
    25921 ctcttcttta cgacgcgtca aggtacagta aaacgtactt ctgtcactgc ttttgctaac
    25981 atccgaagta atggtctgat cgcaattggt ttgaaagaag acgatgagct cgtcaatgtg
    26041 ctactgacag atggtcaatc taatatcatt attggaacac ataacggcta ctctgtcact
    26101 ttcaaagaag aagcagtccg cgatatggga cgtacagctt ccggggtcag agggatacgc
    26161 ctgcgtgagg aagattatgt cgttggggca gccttaatga aggaaaatca agaagtatta
    26221 gtgatcacag aaaaaggtta cggaaaacgg acaaaagtat cggaatatcc agtgaaaggc
    26281 cgcggaggta aagggatcaa aacggcgaac atcactgaaa aaaatggtcc actagctgga
    26341 ttgaccactg tttctggaga tgaagacatc atgctgatta cagataaagg tgtcattatc
    26401 cgtttcaacg tatcgactgt ttctcaaact ggacgttcaa cattaggcgt acgtttgatg
    26461 aagatggaag ctgatacgaa agttgtcacg atggcagcag tcgaaccaga agctgcagaa
    26521 caaacagttg aagaacaagg atcagaagaa taaccatcaa gactaagtgt gtaagctagc
    26581 aagaataacc gattaaaaaa tctggttatt cctgctggct tttttgatta gttgaatttc
    26641 tcttaaaata ttcgaaactg ttcattctgt attgcttttg accaaaatag agtgtataat
    26701 tagagcttgt gagtagtcta attaattaga taactctcct tgctcttagg aacctaagag
    26761 ccgaaagtcc acagggaggt gaatatcaat ggaaaacacg aaatatgaaa tcatgtacat
    26821 tattcgtcca aacattgatg aagaagcaaa aactgcttta atcgaacgtt tcgatgcaat
    26881 cttgaaagat aacggagcag aagttatcga atcaaaagat tgggaaaaac gccgtttcgc
    26941 ttatgaaatg aacggttacc gtgaaggtat ctatcacatc gttaaagtat cttctccatc
    27001 atcagcaaat gctgtcaatg aatttgaccg tcttgctaaa atcaacgacg atatcattcg
    27061 tcacatgatt gttaaagaag aagcttaatt gttgtttcat gtgaaacaat agaaaacgat
    27121 gagaggagat gactattttg attaataatg ttgtattagt tggacgtctg acgaaagatc
    27181 cagatttgcg ttacacagca agtggtacgg cagtcgcaac atttactttg gctgtgaatc
    27241 gtaattttac aaaccaaagc ggcaatcgcg aggctgattt tatcaactgt gtgatttggc
    27301 gcaaatcagc agaaacttta gcaaactatg ctcgtaaagg aacactttta ggtgttactg
    27361 gacgtatcca aactcgttct tatgataatc agcaaggaca acgtgtatac gtgacagaag
    27421 tagtagctga caacttccaa ttgctggaaa gtcgttctgc atctgaaaat cgccaacaaa
    27481 gcggcggatt ccaaagttct ggtcaatcag ctggcggatt tggcggaaac aataactcta
    27541 atcaaacatc tcaatcatcc aacggtatgc ctgatttcga ccgtgatact tctgacccat
    27601 tcggctcaag ttcaacgatc gacatttcag acgatgattt accattctaa acgaatttga
    27661 taggagggaa atagaatggc acaacaaaga agaggcggac gtaaacgtcg taaagtagac
    27721 tacattgctg ctaaccatat tgaatatatc gattataaag atatcgaatt actaaaaaga
    27781 tttatttcag aacgcggtaa aatcttgcca cgtcgtgtaa ctggtacagg cgctaaaaac
    27841 caacgtaaaa ttactgttgc aatcaaacgc gctcgtatca tgggcttgct acctttcgtt
    27901 agcgaagact aataaatcct aggaattgaa tgactgatcc ttttgatcag ctattcaatt
    27961 cctttttttc tgtaaaaata acctctattc gtttagtcgc tcgaatgacg aaagttatgg
    28021 taaaatcaaa ttagtcatat atgggaggaa aattatggaa aataaaaaca atcatttatc
    28081 cccgaaacct ctcttcctta tcctgtttat attaatcgaa gtattggtag tcttaatttt
    28141 accatttcgt tttgttagcg cttcggtcat tctattgatg aatttatggg gtgttcgcta
    28201 ttggttgaaa atgaaacgtt ggcttatttt aaataaacgt gaaaaaatcc agcaggcttc
    28261 gactgtagct gaaaccaatc tagcttatgt ctcagaagtc atgcctgtag gagtgattgc
    28321 ttatcagcca tcaagccaaa aaatcgaatg gctcaatcca ttagcattga aaatcaaaga
    28381 tcaaagtgga ttaacagagg aagaattgct agatactttt tttactgcaa aagaaaaaaa
    28441 gaaaaaccaa gtctatctag cagataaaac atttcagttt gatatggata cagtaaaggg
    28501 tgttgtttat tttaatgata ttacccaaaa ccagttgtta aaactccatc agacgaacag
    28561 tcagccggtg attggtattt tatctattga taactatgca gatgccattg ataaattaga
    28621 tgacaaagag atttcttatt tgaactcttt cgtcactaca ctagtttctg actggatgaa
    28681 tgaataccat gtctttttca aaagaatcaa cgcagaacgg tttttcttcg tagcacagat
    28741 gatcgatctg aaaaagatga tggataaaaa atttgatttg ctggatcgct ttcgagcaga
    28801 agctgaaaaa cagagctcgc ctatcaccct tagtatgggg attgcttatg gctcaagaac
    28861 cctcgatgaa atcggtggag aagctcagaa taatttagat gttgctttag tccgtggagg
    28921 cgaccaagtt gtcgtgaaag aggtaaaaga aggagcaaaa cctgtttatt atggggggaa
    28981 atctgctaag aatgtcaaac gcacgcgtgt tcgttctcga gcgatgagta ccgctttgcg
    29041 tcggattttt gatgaaacag gcgatgtgtt tatcatgggg cacaagtttc cggatatgga
    29101 tgctttaggc tctgcttttg gtgtcagttg tctggcacgc ttcaaccaga aaaaagctta
    29161 catcgttatc aatgagaacg agatcattcc agatgtagaa cggtgtcttg aagaaatcca
    29221 taagcatcct gaattagaaa tgcaactaat ctctccagaa cgtgccatag agctgaaaaa
    29281 agaagaggac ctcctcgtga tggtggatta ccataaacct tctctatcga tctctcaggg
    29341 cctatatgag caatttgata agattgtgat cattgatcac catcgtcgtg gggaagagtt
    29401 tccaagtaaa cctctgctga cttatattga atcttcagct tcttcagctt cagagctagt
    29461 ttctgagctg atccaatacc aaagcagcaa aacaaatcgc ttagatcgtt ttgaggccac
    29521 attattgctt gcagggatgg ttgtagatac aaatagtttc aatgtccgta caactgcacg
    29581 aacttttgac gttgccagct atttaaggtc ttgtggagca gacgcatcct tagtacaata
    29641 tttattgagc tcggatttag cgacatactt ggagatcagc aatttgattg ctggaagcga
    29701 ctatgtgact tctgatattg ttgtggcggc agggacagac gaaaaagagt ataatagtgt
    29761 tatagcagca aaaacagcag atacattgct ttcgatgatc aatatcaatg cagctttcgt
    29821 cattacaaag cggacagacg gcttgattgg gatcagtgcc agaagtaccg gcacgatcaa
    29881 cgtccaaatc atcatggaat ccttaggtgg cggaggacac ttcacaaacg cagctactca
    29941 actggaaaat acgacagttg aggaaacaaa acagaagcta tttgctgcaa tcaaagaaaa
    30001 tatgaatcaa atatatgata aggagtaatc caaatgaaag taattttttt acaagatgta
    30061 aaaggaaaag gtaaaaaagg ggacatcaaa gaagttccag caggttatgc acaaaattat
    30121 ctaatcaaaa ataacttggc aaaagaagca acgaaaagca gtatcagtca gttagcagga
    30181 caaaagaaag cacaagaaaa acacgaagct gaaatcttgg aagaagctaa aaagttaaaa
    30241 gaattcttgg aaaaagaaga tactgtagtc gaattgaagg cgaaagcagg ggaagacagc
    30301 cgtttgtttg gttcgatccc ttcaaaacaa attgctgaag ggttaaacaa acaatacaaa
    30361 atcaaattag ataaacgaaa aatcgaatta gaaaatccga tccgttcact aggctacaca
    30421 aacgtacctg ttaagcttca tcatgaggtg acggctacga tcaaagttca tgtcgtacaa
    30481 gaataacaaa tcttgatagc gatgttcagc tggtaagtat aaccagaagg aaaaaagaca
    30541 aaccgaaatt tatctgagaa aacagctgca gcaatgaaag caaaagctgc aaatcgattt
    30601 cagattgatt tcggtttctt ttttgggaac agcaaaatag cataaagaaa acgagtataa
    30661 taaaagagaa ctattttttt attacacttt cggtacgaat gatgactttt ctctcatttt
    30721 cttgtaaaat gaagcaattg aagacaagga gcgatacgta tgaatgaagt gtggcaggat
    30781 cgaatcccac cgcaaaatat tgaagctgaa caagcggttt taggctctgt ttttcttgac
    30841 gcagaaacga tcattgatgc catggagtac atagagccaa aagactttta tcgtcgtggc
    30901 catcaaatta tttttgagac aatgatcgag ttaaatgatc gtaatgaagc gattgacgtg
    30961 gttactgtca aagaccggct ggaacaagcc aatttgttag aagatgccgg tggtttaagt
    31021 tatctctcag acttggcatt agcagttccg acagcagcaa atatcgtcta ctatgcgaaa
    31081 atcgttgaag aaaaatcatt gctgcgtaca ttgatccaga cagcaacagg aatcgttacg
    31141 aaaggattcg agcaaggaga agaagttcaa acaattttag atgatgccga acgcagtatc
    31201 ttggaggttt cggaaaaaag aaatcgaagc ggctttttat ctattgctga agtactgaac
    31261 tccacgattg aaaatatcga tcgactagct caaaacaatg aagaaatcac aggtctacca
    31321 actggctatc aaggtctgga taaaatgaca gcaggtttcc agcccgaaga actaatcatt
    31381 cttgctgcac gtccagcggt aggtaaaaca gcgtttgcat tgaatattgc tcaaaatgta
    31441 ggaacaaaaa cagatcgttc tgttgcgata tttagtttgg aaatgggggc agaatctctt
    31501 gtaaatcgga tgctttgtgc agaaggttcg attgaggcca gccatttacg tacaggtcag
    31561 ctttcggaag aagaatggtc gaatctgatc gttgcgatgg ggagtctttc tcgagcaaat
    31621 atttttatcg atgatacacc agggatcaaa atctctgaaa tccgtgcgaa atgtcggaaa
    31681 ctagcgcaag aaaaaggcaa tctaggcttg atattgatcg attatttgca gttgattgaa
    31741 ggtaacggta aagagaaccg tcaacaagaa gtatcagata tctctcgcca actgaaaaaa
    31801 ctggcgaaag agctgaaagt acctgtcatt gctctttcac agttatctcg tggagtagaa
    31861 cagcgacaag acaaacgacc agtgctaagt gatattcgtg aatccggatc aattgaacaa
    31921 gatgcggaca tcgtagcctt cttgtatcga gatgattatt atgaaagaga aggcggagat
    31981 gacgatgatg gaaacgagcc aaaaaatgac aacgtcatcg aagtcatcat cgagaagaac
    32041 cgaagcggtg cacgaggaac agttgaacta ctgtttatca aagaatacaa taagttttcg
    32101 tcgctttcgc caagagaaga attttaaaga agaggttgtg agccttgagc aatcccgaga
    32161 gctctcgtaa gaagatgcaa ggagtaaccg caggaacgac tagagtactt aatttaaaca
    32221 gatctccaaa gggattgtga caaaagtctt gtcacagtcc ctttttccga ataaacggtg
    32281 ttcaatcagc tgacccttgg tattagctca gcaaacgtca aaatatgagg aactattttg
    32341 acgtttgttt ctctaatact caggtcaaaa cgctgatctt acgacctctt ttccttaaac
    32401 ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat catcaaacat gagaagcagt
    32461 tttttgaaat tgctccttgt gactcagagt taaacggcag gctcacaacc tctttcttaa
    32521 acggtgttca aaagcttgca tctgcggtaa tacgcccaat caaacgaaaa atatgagaag
    32581 ctatttttgt ttggctgttc ttattacttg atgcaggaca gctttttcac aacctctgat
    32641 tcacggtgtt ccaaaagtag cttcttcgaa aaataagtcg aaatttcaca aaaatttgag
    32701 aagcaatttt cgtacattcc ttcttatttc ttgaagctga ccacttctgt tacaacctct
    32761 tttttaataa ccattctaaa aagataaagt tcgtttttta agcgttttta taaaataaaa
    32821 ataaaaatca ttcgatttta tttgaaatat gtatctatta ttgatacaat ggttcaggat
    32881 taaataatat agaacgaggt gttcacatgt catctgtagt agtagtaggt acgcaatggg
    32941 gcgatgaagg gaaaggaaaa atcacagact ttctcagtga aaatgctgaa gtgattgcac
    33001 ggtaccaagg tggcgacaat gcagggcaca caattaagtt taacggtgtg acttataaac
    33061 tgcatttgat tccttctggg attttttata aagacaagat cagtgtgatc ggaaacggcg
    33121 ttgtagtcaa tcctaaatca ttggttaaag aattagctta tttacatgaa aaaggtgtag
    33181 aaacaagtaa tttgcgtatt tctgaccgcg cgcacgtaat cttgccttat catatcaaat
    33241 tggaccaatt acaagaagat gctaagggcg aaaataagat tggtacaacg attaaaggaa
    33301 tcggacctgc ctatatggat aaagcagctc gtgtaggtat ccgtattgca gacttattag
    33361 ataaagaaat ttttgaagaa cgtcttcgtt tgaatctaga tgacaaaaac cgtcaatttg
    33421 taaaaatgtt tgattcagaa ccacttgatt ttgacgatat ttttgaagaa tattatgaat
    33481 acggacaaca aatcaaacaa tacgtcacag atacttccgt tatcttgaat gatgctttgg
    33541 atgcaggcaa acgcgtgttg tttgaaggcg cacaaggtgt aatgctggat atcgaccagg
    33601 gaacttatcc ttttgttact tcttctaacc cagtagccgg tggggtaaca atcggaagcg
    33661 gtgtagggcc ttcaaaaatc aataaagttg tcggcgtctg caaagcctat acgtcacgtg
    33721 tcggtgatgg accatttcca actgaattat ttgatgaaac aggagaacaa atccgcgaag
    33781 taggacgaga atacggtacg acaactggtc gtccgcgtcg tgtaggctgg tttgattctg
    33841 tagttatgcg ccattctaaa cgagtttcag gtattaccaa cctatcttta aactcgatcg
    33901 atgtattaag tggattggaa acagtaaaaa tttgtacggc ttatgaatta gatggcgaac
    33961 taatctacca ctatccagca agcttgaaag agttgaaccg ctgcaaacca atctatgaag
    34021 aattgccagg ctggtcagaa gacattacag gatgcaaaac acttgcagaa ctgccagaaa
    34081 acgcacgtaa ctatgtacgt cgtatctcag agcttgttgg tgtacgtatc tcaacatttt
    34141 ctgtcggacc tgatagaaca caaacaaaca tcttagaaag cgtctgggcg caaatctaag
    34201 gataaaaacg acgactgaat caggacgttc ttaccagagc gagtcgaagt gtttatctat
    34261 acgataataa taacttgaag aggtagtgaa agagacgttt agccttattg ccgataggct
    34321 gtctcttgaa cactgtttat tcagaataag ggactgtgac aaaaaccgtg tcacagtccc
    34381 tttttccgaa
//
LOCUS       DAZUKC010000025        33503 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000025 DAZUKC010000000
VERSION     DAZUKC010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.026"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(6..122)
                     /locus_tag="ACG80T_001120"
     CDS             complement(6..122)
                     /locus_tag="ACG80T_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFS4840787.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(195..1151)
                     /locus_tag="ACG80T_001121"
     CDS             complement(195..1151)
                     /locus_tag="ACG80T_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS4840788.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            1322..1690
                     /locus_tag="ACG80T_001122"
     CDS             1322..1690
                     /locus_tag="ACG80T_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFS4840789.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            1860..2291
                     /gene="mraZ"
                     /locus_tag="ACG80T_001123"
     CDS             1860..2291
                     /gene="mraZ"
                     /locus_tag="ACG80T_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFS4840790.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            2312..3271
                     /gene="rsmH"
                     /locus_tag="ACG80T_001124"
     CDS             2312..3271
                     /gene="rsmH"
                     /locus_tag="ACG80T_001124"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFS4840791.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            3276..3677
                     /gene="ftsL"
                     /locus_tag="ACG80T_001125"
     CDS             3276..3677
                     /gene="ftsL"
                     /locus_tag="ACG80T_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFS4840792.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRRVK"
     gene            3678..5870
                     /locus_tag="ACG80T_001126"
     CDS             3678..5870
                     /locus_tag="ACG80T_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFS4840793.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            5896..6858
                     /gene="mraY"
                     /locus_tag="ACG80T_001127"
     CDS             5896..6858
                     /gene="mraY"
                     /locus_tag="ACG80T_001127"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFS4840794.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            6872..8233
                     /gene="murD"
                     /locus_tag="ACG80T_001128"
     CDS             6872..8233
                     /gene="murD"
                     /locus_tag="ACG80T_001128"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFS4840795.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            8259..9347
                     /gene="murG"
                     /locus_tag="ACG80T_001129"
     CDS             8259..9347
                     /gene="murG"
                     /locus_tag="ACG80T_001129"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFS4840796.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            9362..10582
                     /locus_tag="ACG80T_001130"
     CDS             9362..10582
                     /locus_tag="ACG80T_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFS4840797.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            10738..12063
                     /gene="ftsA"
                     /locus_tag="ACG80T_001131"
     CDS             10738..12063
                     /gene="ftsA"
                     /locus_tag="ACG80T_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFS4840798.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            12086..13327
                     /gene="ftsZ"
                     /locus_tag="ACG80T_001132"
     CDS             12086..13327
                     /gene="ftsZ"
                     /locus_tag="ACG80T_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFS4840799.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            13344..14021
                     /locus_tag="ACG80T_001133"
     CDS             13344..14021
                     /locus_tag="ACG80T_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFS4840800.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            14035..14634
                     /locus_tag="ACG80T_001134"
     CDS             14035..14634
                     /locus_tag="ACG80T_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFS4840801.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            14715..14936
                     /locus_tag="ACG80T_001135"
     CDS             14715..14936
                     /locus_tag="ACG80T_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFS4840802.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            14985..15767
                     /locus_tag="ACG80T_001136"
     CDS             14985..15767
                     /locus_tag="ACG80T_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFS4840803.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            15788..16495
                     /locus_tag="ACG80T_001137"
     CDS             15788..16495
                     /locus_tag="ACG80T_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFS4840804.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            16757..19555
                     /gene="ileS"
                     /locus_tag="ACG80T_001138"
     CDS             16757..19555
                     /gene="ileS"
                     /locus_tag="ACG80T_001138"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFS4840805.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(19604..21130)
                     /gene="zwf"
                     /locus_tag="ACG80T_001139"
     CDS             complement(19604..21130)
                     /gene="zwf"
                     /locus_tag="ACG80T_001139"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFS4840806.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(21145..21792)
                     /locus_tag="ACG80T_001140"
     CDS             complement(21145..21792)
                     /locus_tag="ACG80T_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFS4840807.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(21976..22305)
                     /locus_tag="ACG80T_001141"
     CDS             complement(21976..22305)
                     /locus_tag="ACG80T_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFS4840808.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            22482..23210
                     /gene="sfsA"
                     /locus_tag="ACG80T_001142"
     CDS             22482..23210
                     /gene="sfsA"
                     /locus_tag="ACG80T_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFS4840809.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            23226..24239
                     /locus_tag="ACG80T_001143"
     CDS             23226..24239
                     /locus_tag="ACG80T_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4840810.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            24239..25516
                     /locus_tag="ACG80T_001144"
     CDS             24239..25516
                     /locus_tag="ACG80T_001144"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS4840811.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            25579..28281
                     /locus_tag="ACG80T_001145"
     CDS             25579..28281
                     /locus_tag="ACG80T_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4840812.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            28433..28750
                     /locus_tag="ACG80T_001146"
     CDS             28433..28750
                     /locus_tag="ACG80T_001146"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4840813.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            28780..29100
                     /locus_tag="ACG80T_001147"
     CDS             28780..29100
                     /locus_tag="ACG80T_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS4840814.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            29208..30668
                     /locus_tag="ACG80T_001148"
     CDS             29208..30668
                     /locus_tag="ACG80T_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4840815.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            30736..30957
                     /locus_tag="ACG80T_001149"
     CDS             30736..30957
                     /locus_tag="ACG80T_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840816.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            30988..31170
                     /locus_tag="ACG80T_001150"
     CDS             30988..31170
                     /locus_tag="ACG80T_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFS4840817.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            31170..31583
                     /locus_tag="ACG80T_001151"
     CDS             31170..31583
                     /locus_tag="ACG80T_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFS4840818.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            31706..32887
                     /locus_tag="ACG80T_001152"
     CDS             31706..32887
                     /locus_tag="ACG80T_001152"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4840819.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(32958..33122)
                     /locus_tag="ACG80T_001153"
     CDS             complement(32958..33122)
                     /locus_tag="ACG80T_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840820.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            33276..33349
                     /locus_tag="ACG80T_001154"
     tRNA            33276..33349
                     /locus_tag="ACG80T_001154"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33310..33312,aa:Arg,seq:cct)
     gene            complement(33418..>33503)
                     /locus_tag="ACG80T_001155"
     CDS             complement(33418..>33503)
                     /locus_tag="ACG80T_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFS4840821.1"
                     /translation="LHIVRELEDQDKEKINTVFDSLYKNDN"
BASE COUNT        11184 a         5721 c         7082 g         9516 t
ORIGIN      
        1 tatttttaca tgacactcat tacagcagtc aatacaacag atccaatcgt taaaatcagc
       61 attgtccaaa tgacaaattt tgttacttta gcaaaagtac ttgtttgttt tttttcacgc
      121 attgttttca acctcgtcat taattagttg attcaagtgt acaatctttt ttccatttcc
      181 acaagagggc ttttttataa aaacttccct ttttttaaat aataggtagt tacgatacaa
      241 ataaataatg ttaatagaac tagccatata aaggcatcat ccctttcagc aataggtaac
      301 ttaatgttca ttccatagat actgccgacg attgtcggta tagttaaaat aatcgttaaa
      361 gaagttaaaa ttttcataat attattcaga ttattcgata cgattgctga aaaagtctga
      421 ttcatttgtt ctactagttt taattgaatg cgagaagtag tcattgcctg ctctgtttcc
      481 accaatacat cacgtaaatg atttttcaaa gcatgatttt cttggaaaag tttattttgc
      541 gctagttctt tcaatgcatc taaattagca gaagtcgctt ctctgaaata aacgaggctt
      601 ttttggatat ccatgatttg ataaagttgt ttgttttcag tggatacttg aagtttggct
      661 tccaaagtat cgcattgaga ttgaagcgta gctaaataac tgttgtaaga gagtgtgacc
      721 tgccaaatga attgaaagaa gatattgaga ttaggcgaaa tatcactttt tggaaaggtc
      781 tggctaaaga ctttctttaa aaacagtggt tcatgattcg taattgtgat aagcttcttt
      841 ttgggcgtca aaataataga gagcgggtat gtatccactt gaaaataacc gctaggactt
      901 gttttagcaa aaggatattg taacaataat aacgctggct ttgtcaactt tgattgattg
      961 aattcttctg tacgggaatt ttctgcatcg tccaatatgg aagtgatgta atctgacgga
     1021 atttcaaatt gatgtgtcag ctgatgaatc tcttcttgat taggacgttc tacacaatac
     1081 cagatcgttt ggtcagtttc ctttccggta ttacgcagtt tctcattatc taatgtaaaa
     1141 tatttgatca tgattttcct cctgttgcct gtttctttta ttataacgga tagggaattg
     1201 gaatacatgg aatgtgtttg taagatatag aatataactg cataaattta aataaaatga
     1261 aacagttgtg taaagtatat acagtatact tgaagcaacg ataaaaaagg agtttacgtt
     1321 catgggcaca ttcacaccac ttatcttgtt ttggtatatt tacccggtaa tcgttttgtt
     1381 tggttgccaa tttcttgtaa aattattttc gttaaatcgt cgttttaaaa taaaagcccc
     1441 tgatttggcg attccttttt tgtggggcgg tatgcatgca ttgtctagaa atacagggac
     1501 aatttctatc ttaccgtttt tctttatttc tgttttgcta atgggaattt gtattgctgt
     1561 tttccaagca tattattatg aagaaattat ttatccgcgt tatttcaaga tgacttggcg
     1621 tttggttttt ctgttgacaa tcgtcttgta cgctgtcttg atcattgtga acatactttc
     1681 ttacctatga aaaaaataga aaagctgtaa aaagtctttt aaaaggcttt ttgcagcttg
     1741 atttttaaaa aaatgtttat gaataaaaaa agttgttccc ggatttccca gaaaaggtgg
     1801 tagaaagtgg gggattgtgg tagactaatt ttagttagtg gaggaatggg gggctttcaa
     1861 tgttgatggg tgaattccaa cataatattg atgcaaaagg acgtttaatc gtgccctcaa
     1921 aacttcgtga agaacttggc gaaaagtttg ttctgacacg cggattggat ggctgtcttt
     1981 tcggctatcc gatgagcgaa tgggaaaatc tggaagctaa actgaacgaa atgccgttgg
     2041 ctaaaaaaga cgcacgtact tttgtgcgat tcttctattc ggctgccact gaatgcgaac
     2101 ttgataaaca aggacgaatc aatataccca gcacgttaag aaattatgca gctttgacaa
     2161 aaggttgcgt agtcatcggt gtatctaacc gaattgaaat ttgggatgag acacgatggc
     2221 aagatttttc tgcagcagca gaagagaact ttgacgaaat tgctgaaagt atgattgatt
     2281 tcggactata agaataggaa gaggctgact aatgactgaa acatttcaac actatacagt
     2341 catgttgaaa gaaacggtag atggattgaa catcaaaccg gatggcgttt atgtagactg
     2401 tacactcggc ggtgccggac atagtgaata tttattaagc caactaagtt ctaaagggca
     2461 tctgtatgcg tttgaccaag atcaaaaagc aatcgatcac gccaagctgc gtttagcttc
     2521 ttatgtcgaa caaggtatgg tcacatttat caaagcgaac ttcagagaat tagaagaacg
     2581 cttgagtgag catgtagata aggtagacgg cattttgtat gatctagggg tttcctctcc
     2641 tcagctagat gaagcagaac gtggatttag ttatcatcaa gatgcaccgt tggatatgcg
     2701 catggatcag tcagctcctt tatccgctta tcacgtaatt aatgaatatt cctaccatga
     2761 attagtaaag attttcttcc gctatggcga ggagaaattt tccaaacaaa ttgctcgtga
     2821 aatcgaacga gtacgaaaaa caaaaccaat ccaaacaact ggtgagcttg tcgaaatcat
     2881 taaacaagtc atcccagctc ccgcaagaag aaaaggcggg catcctgcaa aaagaatttt
     2941 tcaagctgtg cggattgcag taaatgacga actcggtgcc gaagaggcct ctttagaaca
     3001 ggcaatccgg ttattgaaag tcaatggaag aatcagcgta atcacattcc attcattgga
     3061 agacagaatt gtcaaaagta tgtttaaaga atatagtacg gtacaagatc taccaccggg
     3121 acttcctgtc atacctgacg aattccaacc agaactgaaa gtcattactc gtaaaccaat
     3181 ccttccttca gaggaagagc taacagaaaa caatcgatca cgcagtgcaa agttacgtat
     3241 tgcagaaaaa ataagaatga aagaggagta gaatgatggc agaattaaag aaaatgcaag
     3301 attacgaata tgatttacct gaattggtgg atcagccgga acaacaaatg cctgattctt
     3361 cttcgaatca agaaatcatt cttccacaat caccggcaaa acgattaaag cagatttctg
     3421 ctttggaaaa aattgctgtg gcaagcgtgg cgattgctat tgttgcttta tgtgttttaa
     3481 cagttatgct gcgcacaaat atcagcggag ctgaaaaaga aatcacgtca atacaaacag
     3541 aaatcaatga taaaaagcaa gaaaaaacga gtttagaaca agaaaaaaac gaactttctc
     3601 gtactgaacg gattaaacaa atcgcagaag aaaaaggatt gtctattaat gacgacaatt
     3661 taaggagagt gaagtaaatg agtcttaaaa ataaaattcg gcggtttatg gaaaagaaaa
     3721 atctaaatcc gatgaacaat cgcaaaaaag taggtatcat tttatttgct acgagtattg
     3781 gattgttctt tttatttgcc gtccgtttct cgtatatcgt tattggaggt catgttgctg
     3841 gtacctcatt agctgaaaaa acaaaacagt tgtaccaagg aagtgaagta gtaaaagcaa
     3901 aaagaggaac gatttatgat cgtaacggtg tggcgctcgc agaagatgcc tcctcttatt
     3961 caattaaagc gatactctca aaaacatata cctctgggga taaaaaactg tatgttgaag
     4021 aaaagaattt tgataaaata gcagaaatcc tgcataagaa tctttctata gacaaaaaag
     4081 atgcactgaa tattttagag gacggcgcaa aaaaagagct gtatcaagtt gaatttggta
     4141 gctatgggaa aaatatctcc caagaaacca agcaaaacat cgaagcggac atgaaaaaag
     4201 aaggtgtcgc tggtctttat tttgtcgacc atcaggctcg gatgtatcca aatggcgtct
     4261 tttcttctca ttttattgga tacgcagttc cagacaaaga tgagaatggc ttagttggta
     4321 aactaggttt ggagtctgct tacaatgata tcttgagcgg taaagatggt aagattattt
     4381 atcaaaaaga taattttcaa aatcctctgc caggtacagt ggcagaagaa gaaaaagctg
     4441 tcgacggaca agatatctac acgactttag acagcagact acaaagttat ttagaaacac
     4501 tgatggatca agtaaatgaa gaatatcagc cagaagaatt gacagcagtc ctgatgaaag
     4561 caaaaaccgg agaaatcttg gcaatgggac aacgacctac tttcaatcct gaaacaatgg
     4621 aaggattgac tggaaaagat gctatctggc gtaatttctt ggtacaagat agttatgaac
     4681 caggatcaac catgaaagtt ttcactactg cagctgctat cgaagaagga gaattcaacg
     4741 agaatgaaac cttccaatct ggaaaaatcc aagtagaaga tgctacgatc aatgaccatg
     4801 actttggtga aaaaggtgtg ctgaccatgc gtcaagctct ttcatggtca agtaacgtcg
     4861 ggatggtcat actggaacag cgattaggcg gacgatggta taactattta caaaaactgg
     4921 gatttggaca aagtactcat tctggattag atgatgaagt aaacggtgct ttacctacat
     4981 taaatattgt cgaccgagca atgagtgctt atggacaagc ggtaggtgtg acgaatttcc
     5041 aaatgatgaa agctttcact tctattgcga ataacggtac aatgattcaa cctcgatata
     5101 tcagcaaagt ggtggatccc caaactggag aagagcgaac aactcaaaca gaagttctag
     5161 ggcaaccttt ttcaaaagaa acaacagaaa aagtccgtga atatatgaga gatgttgtcg
     5221 aaagcgaaaa ctacggtagt gcttatggtg tatatagtgt acctggatac aatgtatcgg
     5281 caaaaaccgg aacagcccaa atcgcttctg atacaggggg atatcaaaca ggagatacag
     5341 cctacctgta ttcgattgtg gagatggttc cttctgaaga tccagactat gtcttgtatc
     5401 taacaatgaa acaccctaag acatatgatc gaatggcatt ggctaaaatc gcaaatccat
     5461 tgatgaaacg ggcaatggat tttaaagaaa ctgaagaaga ttcagataca gaaacaaaaa
     5521 cagaaaaagt atccgtagcc gattatcgta atcttgaagc ggacgttgca gcagctgatg
     5581 ctcaaaaaag tggactgcaa cctgtagtga tcggtaatgg taaaaaagta caaaaacaat
     5641 ctactgccaa cggtgatcag ttgatctctg gagaaaaact gatcctatat actggtggtg
     5701 ataagctgat gcctgacgta acaggatggt caaaagcaga tatcatgaag ctggggaaaa
     5761 tcctcggtat tgaagttagt tttgatggag atggttattg tgtcaaacaa gaacttgctc
     5821 catatgaaaa aatcactgaa gacaaactta gttttacttt agaagaataa gaagagaaat
     5881 aggagagaaa agagcatgga atggacacaa gcattgattc ccattgtcag cagttgtgca
     5941 atgacaattg ccgcaatgcc gctatttatt ggttattttc aaatgaaaaa gcaaggtcag
     6001 gcgattcgtg aggagggacc aaagtggcac aatagcaaag ccggaactcc tacaatgggc
     6061 ggcttagtgt ttctgatcgg ctctattttg acaggaattt gggttggagc ttggcaaaaa
     6121 cagctaactc ctactctttt catcttgctt tttgtattag cgttatacgg agtaatcggc
     6181 tttcttgatg attttataaa aatattcaag aaaagaaaca tgggactgaa ctcgaagcag
     6241 aaattgattg gacagattct agggggaatc atcttttatc ttgtttaccg ttctgaaggt
     6301 tatccaggtg tattgaattt cttcggacta gatctacctt taggaatcat ttatggtatt
     6361 ttcgctctct tctggcttgt tggattttca aatgcagtaa atctgacaga tggcatcgat
     6421 ggattagttg ctggcttagg aagtatctct tttgcagctt acggtctaat tgcttggcat
     6481 cagcatcagt atgatgtatt aatcatttgc ttaagtgttt taggaggttt actgggtttc
     6541 ttcgtctaca acagaaaacc tgccaagatt tttatgggag atgtcggttc attagcgttg
     6601 ggcggacttt tagcggcaat ttctataatg ctaaaccaag aatggacatt gcttttgatt
     6661 ggtttgatct atgttatgga aacagcaagt gtgatgcttc aagttacttc ctttaagctt
     6721 acaggaaaac gaatttttaa aatgtctcca attcatcatc actttgaaat gtgcggatgg
     6781 tcggagtgga agattgatac agttttttgg ttgatcagca ttgtgacttc gttgatcaca
     6841 ttatggatcg tttggtagga gatgacggga aatgaaaaaa atatcaacat atgaaaataa
     6901 gaaagtgctt gtcttaggac tcgcaaaaag cggagtaagt gcggccaaat tattacatga
     6961 attgggcgca cttgtcaccg ttaatgatgg aaaacctttt gacgaaaatc cggaagcaca
     7021 agagctgctt tctttaggga taaaagtaat aacaggtagt catccaatcg aattgttgga
     7081 tgaagaattt tcattgatgg tcaaaaatcc agggattccc tacagtcatc ctttagtagc
     7141 aaaagcccaa gaaatgggta ttccggtaat tacggaagtt gagctagctt acgaagtagc
     7201 ggaatgccca attattggga ttacaggaac gaacggaaaa acaacgacaa caacgatgac
     7261 aggactacta ctgaatgcag gagcagatca aggaattgca cgtttagcgg gtaatattgg
     7321 gtatccagca agtggtgtag cgcaagaagc aaaatcagaa gataaaattg tgatggaatt
     7381 gtctagcttc caactcatgg gaatcacaga tttccgccct catatagcgg tgattaccaa
     7441 tatctatgaa gcacatattg attatcatgg tacaagaaaa gaatatgtca aagctaaatg
     7501 gaaccttcaa aaaaacatga cagaaaaaga ttatctgatc cttaactgga atcagtcaga
     7561 actacaagaa ttagcgcaaa gaacaaaagc acgcgtactg cctttctcga caaaagaagt
     7621 gttggaagac ggtgtgtatg ctgacgatta ctcaatatac tataaaaaag aaaaaataat
     7681 ggaaatatct gaacttggcg taccaggtaa gcataatgta gaaaatgcac tagctgctat
     7741 ttctgtagca aaattgtatg gtatatcaaa tgaagctata cgagaaacgt tgcatttttt
     7801 ccatggcgta ccacatcgta cgcaatatgt aggagaaatc caaggcagaa aattttataa
     7861 tgattcaaaa gcaaccaata ttttagcgac taaaatggct ctaagtggat ttgaaacgtc
     7921 taaggtcgtg ttattggctg gcggattaga cagaggaaat acatttgatg aattaattcc
     7981 ttcattaaaa ggaatcaaag caatggtcgt atttggccaa acaaaagaaa agttgatgga
     8041 tgctggaaaa aaagcaggca tcgagacaat agtaacagcc gattctgtag aacaagcagt
     8101 tcctttagca ttagaaaaca gtacagatgg tgatgtagtt ttattatcac cagcgaatgc
     8161 cagctgggat caatacccaa attttgaaac tagaggaaac agattcatgg aagctgtgaa
     8221 ccgattaaaa tagaaaaagt agaagcgagc gataaaaaat gaaaattttg gttacaggcg
     8281 gaggaacagg gggacatatt tatccagctc ttgcctttgt caattatgta aagactcaag
     8341 aaccgaatgc tgaattcatg tatgtgggag caaaacgagg tctagaaaac aaaatcgtcc
     8401 ctgatacagg aatgcctttc cataccttgg agattcaagg atttaaacga aaaatttcca
     8461 tgcataacat caagacggtc cgattgtttc ttaagagtat ccgtcaagca aaaaaaatcc
     8521 tcagtgattt tcaaccagat atcgttatcg gtactggagg atatgtttcc ggagcggtag
     8581 tgtacgctgc agccaaaatg ggtattccaa cagttatcca tgaacaaaac agtgtaccag
     8641 gtgttaccaa taaattttta agccgatatg tggatcggat cgctctttca tttaaagatg
     8701 cggcttcctt ttttcctgaa aacaaagcag ttcttgtagg taatcctcga gcacaagaag
     8761 tagcagatac taaaaaatca gatattttga aaacatttga tttggatcca gaaaagaaaa
     8821 cagtcttgat ttttggcggt agtcaaggag cacttaaaat caatcaagca gtatcagttt
     8881 ttttaagtga atatattcct tcttcttacc aagttttgta tgcttcagga gatagatatt
     8941 atgaagagat cctaaataaa ataaatcctg aaatacaaaa tattagtgtc cagccttata
     9001 tcaaaaatat ggcagaggtc atggcttcca gtgatctgct ggtggggcgt gctggtgcaa
     9061 cttcgatcgc agaattcaca gctctggggt taccggcagt gttggttccc agcccatatg
     9121 ttacgaatga tcatcagact aaaaatgcaa tgagtcttgt tcacgcagga gcagcaaaaa
     9181 tgattgcaga taatgaacta acaggtgaat cactaagtca gacagtcaac gaaatcatgg
     9241 gtgatgaaga attgcaaaag caaatgtgcc gagcttctaa agaacaagga atccctgatg
     9301 cttcaaaacg tctttatgac ttagtaaaac aaataatcaa aaaataaagg cggtggtctt
     9361 agtgagtaag aaaaaagaac caaaaaaatt gtctcatccg tcttctgatg aaaacaattt
     9421 gactccttgg caaaaggcta accgaaaata tctggcagaa caagagatga aaaaccaaga
     9481 gccaaaaaaa gaagacataa cattagatac acccctttca gattctgaag aagaaaaaac
     9541 ggttcaagag gaacagcaag aagaaagcca gtccgagaaa aaaatcgaat tttttgaaga
     9601 gatcaagcaa aagaaaaacg gtggaccata taacggttct tttttgaacc ggctgcccaa
     9661 tttaaaaagt caacggcaca aagtactatt tcgtcggtta gctttgatca tcactgtatt
     9721 gggtataccg ctgatttttt tgatctatta tgtttctcct tatagcaaat tacaagcagt
     9781 tgcagtatca ggcaataaga cagtcaacag ccaagaaatc atctctgata caaaattgtc
     9841 attaggagaa aatgtttggg gacagtattt tcatcgatct acttatatcg atcgccttaa
     9901 aaaagcacag ccaagaatcg aaacagcaaa tatacatttc aaagggatga atgaattcga
     9961 tttggatgta acagaatata aagagattgc tttgatcgca aaaaacaatc aatactaccc
    10021 agtgattgaa aatggaacag tattagatga aaaagtagca aatcctacaa aaaatctccc
    10081 tatcttggaa gagttcaaag actctgcaaa aatcaaagaa ttaacaaagc aatacaatca
    10141 gttatcatca gaattacaaa aagcaatttc tgaaatcaag tacacgccaa gagcaagcaa
    10201 taaaaatctg atccaattga atatgaatga cggcaatcaa gttattgtaa atatcaataa
    10261 tcttgcgaac caaatgaaat attattcgca agtagccaaa gatatggatg aaaaaggtgt
    10321 gatcgacatg gaagttggta tcttctcgca tccttacggt agcactcaag aaacggaaga
    10381 atcgggccaa tcggaagaaa caactgaaag tgaagaaaat gcggatcaaa caaacgaatc
    10441 aagtgaagag gcagaaacgc aaaaagcaca aacacctgga tcgtctagca caaacggatc
    10501 atctgattca aatgattcaa gtgaaacaag tcaaatagaa gataatccaa gtacaactcc
    10561 gtcaagttta aatgaaaatt gataaaacaa ctttaaaaag taagaatgta ctttctaaaa
    10621 accagtaatt tatggtagaa ttggaagcag tgaatatatc tattaataag tatattttaa
    10681 aacggacgct agttgttcgc ttgtatatgg aatagtaagt aggagggaat cccattcatg
    10741 gcaaaaacag gaatgtatgt aggccttgat attggaacga catctgttaa agttgtcgtg
    10801 gctgaatata tcgacagcca aatgaatatt attggagtag gaaacgcaaa atcagaaggg
    10861 attaaccgag gcattatcgt tgatatcgat aaaacggtcc aagcgataca acgagcagta
    10921 cgacaagcag aagaaaaagc aggtatccag atcaaaggcg taagtgtcgg tttgccagca
    10981 aacatgcttg aagtagaaaa ttgccaggga atgattgcag ttaacggaga ttcaaaggaa
    11041 atcaccgatg aagatgtcag aaatgtggct tcagctgccc ttgttcgttc aatacctcct
    11101 gaacgtcaaa tcgtgtcaat attgccacaa gattttactg tcgatgggtt cgagggaatc
    11161 aaagatcctc gtggaatgat cggcgtaaga ttagaaatgt acggtctgtt gtttacagga
    11221 cctaaaacga tcatccataa catacgtaaa tgcgtggaaa atgccggatt aatcgtcaac
    11281 gaaatggtga ttactccttt agcgctgact gaatcaattc tttctgacgg agaaaaagat
    11341 tttggtacga tcgtcattga tatgggtggc ggccaaacaa caacggctgt tatgcatgat
    11401 aagcaattga agtttaccaa tcttgaccaa gaaggtggcg aatttgtcac taaagatatc
    11461 tctattgtat taaatacgtc atttaacaat gcagaagcgt tgaaaataaa ctacggggat
    11521 gcatacccag aacggacatc accagatgaa gaatttccag tagatgtgat tggtcagtca
    11581 gaaccagtga aagtcgatga acgctacttg tcagaaatta tttcagctcg aatggaacag
    11641 attttcaaca aagcaaaaga tgcattggac caaatcgaag cattagagtt gccaggtggc
    11701 gtcgtattga caggcggagc tgctagtctt cctggtgttg tggacttggc acaagaaatc
    11761 ttcggcgtaa atgtaaaatt gtatgttcca aatcacatgg ggctgagaaa cccggtattt
    11821 acaaatgtaa taagtatcgt ggattattca gcaaacttga gtgaagtgta tcaattggca
    11881 aaaattgctg ttacgggtga aacctcagca gctcgtcaaa tggtagttga acaagaaaca
    11941 acaaacacat atgagagcta tgaagcacct gaagaaacaa tctacgatga gccggaacca
    12001 aaagagtccg gagaaaatgt aaaaaataaa atcaagggtt tctttacaaa tatttttgat
    12061 taattcatcc tgggaggaat aagatatgga attctcaatt gataataata tcaatgacgg
    12121 cgcagtaatc aaagtcatcg gagtcggtgg tggcggtgga aatgctgtaa accgaatgat
    12181 tgaagaaaac gttaaaggtg ttgaattcat tacagccaac acagacgtgc aagcattaaa
    12241 aaattcaaaa gctgaaacag ttatccaatt aggccccaaa tatactcgtg gtttaggtgc
    12301 tggttctcag ccagaagttg gacaaaaagc ggcagaagaa agtgaacaat ccttacgcga
    12361 agctttagac ggtgcagata tgattttcat cactgcagga atgggtggag gaaccggtac
    12421 aggtgctgca ccaatcgttg caggtatcgc gaaagaattg ggcgcattga ctgttggtgt
    12481 cgtgacacgt ccattcactt ttgaaggacc gaaacgtggc cgttttgctg cagaaggtat
    12541 cgctcgtttg aaagaaaatg tggatacatt attaattatc tcaaataacc gtttattaga
    12601 agttgtagat aaaaagacac cgatgcttga agctttccgt gaagcagata atgttttacg
    12661 acaaggtgta caaggtatct ctgatttgat cacagcgcca ggttacgtta accttgactt
    12721 tgctgacgtg aaaacagtga tggaaaacca aggtacagca ttgatgggga tcggtgtggc
    12781 tagtggagaa gatcgcgtag tagaagcaac gaaaaaagca atctcctctc cattgcttga
    12841 aacatctatt gatggagctg aacaagtact tcttaacatc acaggcggtt tggatatgac
    12901 attgttcgaa gcacaagatg cttcagatat cgtagcaaat gcagcaactg gcgatgtaaa
    12961 tatcattcta ggtacatcta tcaatgaaga aatgggcgat gaaatccgtg tgacggtcat
    13021 cgcaacaggt atcgatgaat caaaaaagga acgcaagtct agccgaccag ctagacaagc
    13081 acaaatgcaa tcacctgctc aaaagactgt attagatatg gatcaagcta aaccaacttc
    13141 ttcagaagaa gaaaacagtt ttggcgattg ggatatcaga cgtgaacaaa atgttcgtcc
    13201 tagagttgat gattccaatt ttgacaatat cgaaaaaaaa gaatttgata cattcaaccg
    13261 cgaagaaaca aaatctcatg gcgatgatga attaaatacg ccaccgtttt tccgtcgtaa
    13321 aaaataaagg agaggaagag cacatgattg ctgacaactt gcagaaaatc aagcaagaaa
    13381 ttcaggattc gtgtgctctt gttcatcgtg atccgaaaga agttacatta gtagctgtaa
    13441 caaaatctgt tgaatccgct caagctgaag aattggttag agctggcgtt atgaattgtg
    13501 cagaaaaccg agtagataaa ttattagaaa agaaacaaga tttatttgat tttcctgaaa
    13561 taaagtggca tttaattggt aatttacagc gcagaaaggt aaaattgatt gtaaatgaaa
    13621 ttgattactt tcatgcgttg gacagtttaa gattagcaga agaaatccag aagcggttag
    13681 agaaagaatt atcctgtttt attgaagtaa atgtttctgg tgaggaatca aaacatggaa
    13741 tttcaccaga tgagctttta ccgtttgtgg aatctttagc tgcctgcaca aaaatcaagg
    13801 ttgttgggtt gatgactatg gcgcctaaag aagcttctga tgagcaaatc cataaagtgt
    13861 ttggtacgct gaaagaattg cgtgatcttg ttcaagcaaa aggttatgcc catgcaccat
    13921 gtagtgaatt aagtatgggg atgagtcaag actttcaaat tgcgattgaa gaaggtgcaa
    13981 catttatccg tgtaggaaca gcgctattca aagacgaata aaggaggaac aaaaatgccg
    14041 cttttaaaca gaggagcaat tgctaatttt ttcggattag ctgatgagga agagtacgaa
    14101 taccaagatt atcctgaaac aacaaaaaag gctgtacagc caactaaaaa aaatacaaca
    14161 cagaatcaat caactcattc tgtatctgct gcgaatactc atgaaaaaca aaaaacggcg
    14221 acttcacgtc ctgtaacgca tgaaagtatt cagccggctg aaaagaaaaa agtaattgaa
    14281 ctacatcaat cacctacaaa aactgtagcg aaagaaagaa cagcttcaag aacctctaca
    14341 caacctaaag caaatcttgc tggaaaaatc actgtaatgg aaccaagagc ttattctgaa
    14401 gctatggcga ttgctaaacg aatcattgct ggagaagcaa ccttagtgaa ttttcattta
    14461 gtagaagaaa aacaagcgag aagaatcgtt gattttctaa caggaacagt ttatgcacta
    14521 gatggagata tcaaacgtgt tggagatgag atttttcttt gtacaccgac tgggatggag
    14581 attgacagtt caaccgctca atcttttgca gatgcgaatt tgtttgatct gtaagaggag
    14641 gaactacgat ttatactatt gcactattac ttcatttact tcaacgggca gcctatttct
    14701 atagcattat cttgatggta tatgcattgc tctcttggtt tccaggcggg tatcaatcag
    14761 ggctaggacg attattagct aagatctgcg aaccgtatat cagtttgttc gatcgtctgc
    14821 ctttgaggat tggacccttg gatttcacaa ttttaggggc aatcctgatc ttgaacttag
    14881 ccgtacaagg tttagcacag atcgttatat ggataactgt ccatttagtt tattgattga
    14941 agcaacaaaa tctttattct cagaatagat aggaggggaa aattttggat gcaaatgtat
    15001 accagcactt tcgggcagat gaacgtacat ttatcgattc ggtaggcgat tggattgaac
    15061 aggttcaagg acaatatgca ccctatctga ccgaatttct tgacccaaga caatcctata
    15121 ttttagaaac actgattcga caaagcagtg atttgcgttt catgttttat ggcggatatg
    15181 aacaggcaga aagaaaaaga tgtttgattt tccccgatta ttacgaacct gtagaggaag
    15241 attttgaaac agcattgtat gaaatccatt atcctagcaa atttgccagc ctaagtcatg
    15301 ggaaaatcct tggaacttta gtgggaacgg ggatcaaacg ctcttacttt ggcgatatca
    15361 tctcggacgg tgatcgctgg caagtattta ttgcaaatga ggtggatcat tttgttgctg
    15421 cacaagtaac aaagattgga aaggttgctg tacgcttgga accaatcaaa tacacagaaa
    15481 tgattatgcc gaaagatagc tggacacaag aaagaggaac agttagttcc atgcgactag
    15541 acagtgtgat ctctgagatt tataatattt ccagacagcg gtccaagcag ctgatcgaag
    15601 ctggtaaagt aaaagtgaac tggacagaaa atacaagacc tgattttgta cttgaactat
    15661 tagatattat ttcagtcaga ggttatggca gagtccagat ccaagaactt gaggggaaga
    15721 cgaaaaaaga aaagtacagg gtactgttcg gtgtattacg taaataaaat aaaaaggtgg
    15781 tttactcatg gctttaactc cattagatat ccaaaataag acattcccga caaaaatgcg
    15841 gggatataat caagacgaag tagatgattt cttagattta gttgttcgtg actatgagga
    15901 attgactcaa agaaatcgag aattagaaaa agcagtcaaa cattctgaag aaaaacttga
    15961 gtacttcaat gaattaaaag atgctttgaa ccaatcaatc atcgtagctc aagatacggc
    16021 tgataaagta aaaacaagtg caagcaagga atccgaagtg atcgttactt ctgcacaaaa
    16081 caaagcagat gagttagttg cgaacgctga aaaacgtgca catcaattaa caactgatgc
    16141 agaagaaaaa gcaagaaaaa tcttgacaga tgcaacagaa aaagcaagac agcttgcgac
    16201 agaaactgaa gatcttaaga aaaaaacacg agtattccat caacgtatca gcttgatgct
    16261 tgagtctcaa ttagaacaag tgaaaagtcc ggaatgggat gaaattttac aaccattctc
    16321 cagctacgtg actgacagcc atgaagtgat caaagaagtt ttagcaaaac agcttgacaa
    16381 tgaaaatgat actgatgtaa actcagacac aggagtcata gaagcaccta tgacggcatt
    16441 tgatacacag gctattgata tctcgatcgt gcctggtgaa aatgaaaacg agtagaacag
    16501 aaaagcactt tgttattctt aaagcgagcc gatgagagtg gaagttcggt aagacactgc
    16561 atggtgaaag atcctctact tgagacctgc ttttccgaaa ttagagtaag aagcagcggc
    16621 tgacctcgtt attggtcaca gagagtttgc actagataga tgcaactaaa tttgggtggt
    16681 accacggcat gttcgtccct tgcggctgaa catgcttttt tgtgtccgga aaacaaataa
    16741 aggagtgtat ggttgaatga aaatgaaaga aaccttgcaa ttaggaaaaa caaaattccc
    16801 tatgcgtggt aatttgccaa accgagaagg cgagtggcaa aaagaatggg aagaaaatcg
    16861 aatctaccaa aaaagacaag agttgaacga aggaaaacct agctttgttc ttcatgatgg
    16921 acctccatat gccaatggta atatccattt aggacattca ttgaataaaa tcagtaaaga
    16981 cattatcatt cgttcaaaat caatgtctgg ctatcgtgca ccatatgttc caggatggga
    17041 tacacatgga ttgccgattg aacaagtatt ggctaataaa ggcgtgaaac gtaaagaaat
    17101 gtccatggca gaatacttgg aaaaatgtcg tgaatatgca ctttcccaag tagataagca
    17161 acgagcagat ttcaaacgcc taggagttgc aggagattgg gaacatcctt atgttacatt
    17221 agatccttct tatgaagctg cacaagtacg cgtatttggt aaaatggctg aaaaaagcta
    17281 tatttacaaa ggattgaaac caatttactg gtctccgtca agtgagtctt ctttagcgga
    17341 agctgaaatt gaatataaag atgtaaaatc tccatctatt tttgtagctt tcaatgtcaa
    17401 agacggcaaa gatattcttg atgaagatac agcttttgtc atttggacga caacaccatg
    17461 gacattgcca gcaaaccaag gtattgctgt aaatccaaaa tttacgtatg tattggtgga
    17521 agccgatggc cgtaagtttg tcgtagcgaa agatttgcta gaaacagtcc aatcagaaat
    17581 cggttgggaa aatgtaacag ttctaaaaga atttgctgga caagaaatgg aatatatgac
    17641 tgcaagtcat ccattctatg aacgtgaatc acttgtgatt ttaggcgatc atgttacttt
    17701 agacgctggt actggattag tccatactgc tccaggacat ggggaagatg actatatcgc
    17761 tggtagcaaa tataaactat ctgtagtgtc acctgtagac tcaaaaggat tgtttacaga
    17821 agaagcacct ggatttgaag gtatctttta tgacaaagca aatcctatga tcactgatct
    17881 attaaaagaa aaaggagctt tgctgaagct agatttcttt actcatagtt atcctcacga
    17941 ttggcggacg aaaaagcctg tgatctatcg ggcaactcca caatggtttg catctatcga
    18001 taaattccgt cagaatattt tggatgaagt agaaaaagta gagtgggtta tcccatgggg
    18061 taaaacacgt ttgtataata tgatccgtga ccgcggcgat tgggtaattt cccgtcagcg
    18121 tgcatgggga gtaccacttc cgattttcta tgctgaaaat ggagaagcaa tcatcacacc
    18181 agaaacaatc gaacatgtgg caaaattatt tgctgaacat ggatcaatta tctggtttga
    18241 aaaagaagcg aaagaacttc ttcctgaagg atttacccac ccaggttctc ctaatggcga
    18301 atttacaaaa gaaaaagata tcatggatgt ctggtttgat tctggttctt ctcatgaagc
    18361 tgttttacgt cagcgaccag aattatcatt cccagcagat atgtatttag aaggatcaga
    18421 ccaatatcgt ggctggttca actcaagtat cacgactagt gtggctatca atggtgtagc
    18481 accatacaaa tcagttattt ctcaaggatt tgctttagat ggtgaaggcc gtaagatgag
    18541 taaatcttta ggaaacatca ttgcaccaga aaaagtgatc aaacaatttg gtgcagatat
    18601 ccttcgctta tgggtatcta gtgtagatta tgaagcagat gttcgtgttt ctatggatat
    18661 cttaagtcaa gtggcagaag tatatcgtaa aatcagaaat acgatgcgtt tcttattagc
    18721 gaatacagaa gatttcgatc ctaaaaaaga tactgtttcg tacaatgatt tacgttctgt
    18781 agacaaatat atgaccgttc gattgaacca agtaatcaaa gaaatccgag aagaagggta
    18841 cgacaaatat aacttcatgc atatttaccg tacagttatg aacttcttga cggttgatct
    18901 atcgtcattc tacctggatt ttgctaaaga tgtagtttat attgaagcag aagatgatta
    18961 tcaacgtcgt tgcatgcaaa ctgttttcta tcaaacagct gtagctttga caaaattatt
    19021 gactcctatc attccgcata cagcagaaga aatctggtca ttcctgaaag aagaagagga
    19081 gtacgttcag ttaagcgagt ttcctggtta tgaagagttt gcaaaccaag aagaattgat
    19141 ggatacctgg actgctttca tggacttccg tgataatgtc ttaaaagcat tggaagaagc
    19201 aagaaacagc aaattgattg gtaaatcttt ggaagcaaaa ctaactgttt atccaaaaca
    19261 acaagttcgt gaaatgttaa aagctttgga tgcagatatt gcacaattat tgatcgtttc
    19321 accagactac tttgaagtta tggcagcaga tgaacaagtg ccagataacg ctatggtatt
    19381 tgatgatgtt gctattacag tggaaaaagc agacggggaa acttgcgatc gctgtcgcca
    19441 agttcgtaaa gatgtaggag tagatgaaaa acttcctcat ctatgcggtc gttgtgcaaa
    19501 aatcgttgaa gacttctatc ctgaagctgt agctgaagga ttcgaagaaa agtaaaaggg
    19561 aaaagggggt gtgacatgtt ctttgtcaca cccccttttg ttatcaatcg tttaattttc
    19621 cacgttcacg ataccagtta tctggttgcc agatccattc gaatccgtct ttttcaagta
    19681 gttcgaaagc tgctttaggt cccattgatc cagctttgta gttgggaaaa tccggttgtg
    19741 tttggtccca agcttttcga atgacatcca cgatatgcca agattgagcg acttcttccc
    19801 aatgagagaa gttcgttcca tcaccattca acgcatctaa tagtaatttt tcgtaagctt
    19861 ctgggctatt ttcaaccatt tctgcactat ttctaaattc caatttgatt ggttccgttt
    19921 caaatccttg ccctacttct ttcccattca aggataaaga aaatccttca gttggctgaa
    19981 tatagatcgt tagaacatta ggtggaagtg tcgtatcgtc acatggttca tcgacagatg
    20041 ttttgaatac attaacagga acttgtttga atacgatgtt gatccgagtc cctttttcag
    20101 tcaaacgttt gcctgtacgt acatagaaag gaacacctga ccaacggaag ttatcgatag
    20161 tgaatttgcc tgcgacgaat gtttcagtct ctgattgagc atccactttg tcttcttcgc
    20221 gatagccctt gaattgtaca ccatccaatt caccttgagc atattgcccg cgaacgaagt
    20281 tttctaacac ttcttcttcg ttataaatgc ggatagcttt caatgctttg atcttttctg
    20341 tacggatttc tttttcggaa aatgctactg gcggttccat tgcaagtaat gctacaacct
    20401 gtaaaatatg attttgcacc atgtctttaa gtgcgccact atgatcgtag tagcccccgc
    20461 gatcttctac accgaggttt tcgccaaaag taatttggat attgtcgata tagcgattgt
    20521 tccattgagc ctcaaaaata ttattggcaa aacggatagc tgagatgttt tggatcattt
    20581 cttttcctaa ataatgatca atcctgaaaa tatcttgttc tgggaatact tctctgattt
    20641 gctggttcaa ttcaaaagca gactggtaat cagaaccgaa cggtttttct atgatcaaac
    20701 gattgaagcc attttttgtc ataatatttt gtgatttcaa atgagaaacg atcgttccaa
    20761 aaaattgagg tgccatagcc aaataataga cacggttacc gtttaattga tagtgtccat
    20821 ctaatcggtc agacaattct tttaatgtct gataatgcgt tgaatcattt acatcatggg
    20881 attgatagta aaaatggctt gcaaaggtag ttgcttcttc atctgaagga ttcaatgcac
    20941 ggattgtttc tttgacaact tcacggtagt gctcatcgct ccagggacgg cgagcggttc
    21001 cgattacagc aaaatgctcg cctaaatttc cttttctgta aaggcggaac aaagacgggt
    21061 acaatttacg ctgggctagg tcacctgtac cgccgaatat cgtaaacaat acattttttt
    21121 cattagacat gtggatacac tcctttatat tttatcgaca aaaatcgtgc tggccgcctt
    21181 aaagctgacg gggattttct ttctatcatt ttggatagtg atcggtcctt cataagctgc
    21241 gatgtcaacg atctcatatt cttcgttgat attcaggtca atagtgacta agtaatccaa
    21301 caattctttt tcatcaagca ctcgtgcgat acgcactttt gtaccgacag gataggactc
    21361 caatgtttgt cggcgttctt catggacaac ttcctctttt ttaggaatcg atcctccatg
    21421 aggacaatgt tcaggatgat tcaaatagtt ttctaaatga tctgcaagat gttccgaagt
    21481 cacatgttcc aatacttcag catcatcatg gacttcattc cagctatagt ctaaatgttc
    21541 aactaaaaaa acttcccata agcgatgttt gcgaatcaga ctgctagctt tttgcagtcc
    21601 gcttgcagtc aattgtacac cttgataagg ggaatgttct acaagctctt ctttcacaag
    21661 tttagaaatc atttcactga cagatgcagc tgagacatct agactagata cgatttgttt
    21721 attgttgact ttcgttgtat ctccacctag ttctaaaatg attttcaaat aatcttctcg
    21781 atttggtgtc atattcgatc atcctttcat ggtaaaaaac catcattagt aagtgtaaca
    21841 aaaaaaacgt taattgacta tttttctaaa taaaaaacaa aggattttta gacgttttta
    21901 caacaaaaaa cgaaagatga tagagaagac tcattctttc gtttataaag gatatatata
    21961 tacattggga gggatctatt tttcagataa caacttatat gaagtgtctg cagattcgtc
    22021 ataaagagtt tgttcgggat aagaaaagat aagataaaaa ccatactgat cacgtacgac
    22081 taaacgatat tcatctattt ctaccacaga tgtgccaaga gactgaccgt tgatcaaaaa
    22141 cgtgtaatca gatgtaatct gtaattgttg tttttgctgg ctatcactaa agaaccatgt
    22201 accttgatac tttgagagtc cttctgactt ttccgcttgt tttttctttt tcaagtaaat
    22261 aaaacttgag agaacggtaa tgagtgaacc aagaaaatag atcatttttt ttatcaccaa
    22321 atcactcctt tatgagaaaa taatcgcata gacatattct ttcttcttta tttcattgta
    22381 ccgaataaaa gctaaaattt ctctaagact ttcgactggt ttttttactg tttttctaca
    22441 aattatgata ggatacatag taggaagaaa ggtgacataa tttgaagtat cagcaagtgg
    22501 aaatagcttt ttttattgac cgcccgaatc ggtttatagc acattgtatg aaaaaagacg
    22561 gagaaataat tattgcgcat gtcaaaaata caggtagagg aaaagaggtc ctgataccag
    22621 gcgctgaagt ggctattgta tttgctcctg gacctaaacg gaaaaccgcc tatgatctga
    22681 ttgctgtaaa aaagaagggt gactggttta atatcgatag tcaattgcct aatcggctgg
    22741 caattgatgg tattttagac ggcacgatcc ttttaccagg attaactggt ccatttacgg
    22801 tatacaagcg tgaagtaact tatcttcact ccaaatttga tatttattta gagacggtgg
    22861 tcggacaaaa agcatttgtt gaagtcaaag gtatgacact tgaaaataaa gcgattggtg
    22921 cttttcctga tgcgcccaca ctcagaggat tgaaacatat aggagaatta acgtatgccg
    22981 cacaagatgg ttatgccgct tatgtcttgt ttattgctca gttcgaacat cttcatctgg
    23041 caacgatcca tgaagaaatg caaccagcac tagctgatat ggttcgacat gcgcagcaaa
    23101 gcggagttca aatcctcgcc tataattgtc aagtatcggc agatgaggta actgtcaagc
    23161 aggccattcc atttgatgta gatatccctt ttgaagatcc tatacaatag aaagaaggta
    23221 aaataatgat caacttaggt attatcggaa caaattggat tactcatcaa tttgtccaag
    23281 cagctttaag cacaaaaaaa tatgaattga cagcggtata ttctagacat ttggaaaaag
    23341 cacaggaatt tggttctcat tatgaagggg atatcgaatt cgcaacagat ttgaagactt
    23401 ttttcaatat tactcatatg gacaccattt atattgcttc tccgaatagt cttcacttta
    23461 gtcaagctaa gcaagcaatt ttggcaggaa aaaatgtgat agttgaaaag ccagcttttt
    23521 cgacgccaaa agaaatggat gaaatcatcc atttggcaaa tgaacacaat gtgtattttt
    23581 ttgaagcagc aagaaatatc catgaatcag gttttaaaaa gattgccgat tacttaccat
    23641 taaaagacga gataatcggt gcaaatttca gctatatgaa atattcttcg cgctatgacc
    23701 aagtattaga tggagaagaa ccgaatatct tctcgactca tttctcaggt ggtgcattga
    23761 tggatttagg tgtttacctt gtatatgcag ctgttgcttg gtttggaatg ccaaaagaag
    23821 tccattattt cccaagaaag atttctactg gtgtagatgg tcttggatgg gggatcttac
    23881 gttatgagaa ttttgatgta tcgatccagc caggtaaaat cggtgattct tatttaccgt
    23941 cagagattta ttttgactca ggaacgctag tgatgaatgg agtcaatgca attgaacaag
    24001 cacaatttca tgatcgcgcg cataaagaaa tcattgattt ggcggtaaca gctgcagaaa
    24061 atccaatggc agatgaagca gaggattttg ccaatgtcat ggctcatccg aaagatccag
    24121 cttggggatt gttgtatgaa gagtggatcg agctagctag aaatgtgaac caagtgattt
    24181 atgatttaag aaaaaatggc ggcatcaaat tcgatgcaga taatgaggaa gatttttaat
    24241 gaacgaattt gtacaagtat ttcccgaaaa ttggcagaaa aaatatgagg ataaagggtt
    24301 tatctcacct tctcttattc aacgagcagt ttttcagcca ctaagtaata aacaaagtat
    24361 agtagcggtt tctcctacag gttcaggtaa aacactggct tatctttggc ctctactact
    24421 aaacgtggaa ccaggtgaag caagtgcgtt ggtcattttt gcttcttctc aagaactagc
    24481 tattcaagta gcagatgttg cacgtgaatg gggaaaagac aaagagttga aagtccaatc
    24541 tttggtgggc ggagccaacg tcaaacgtca gattgagggt ttgaaggaaa aaccagaaat
    24601 cttgattggt acaccaggaa gaatattgga attgatgaaa gcaaaaaaaa taaaagcaca
    24661 tcaagtgaaa acaatggtat ttgatgaagc agaccagcta tttgacgctg ggaacagcca
    24721 aatcattgat caaattcttc atcaagctcc aacagagtat cagctagctt ttttttcagc
    24781 gacagcagat cgttcccttg aaagcattga aaaaatcact gggaaaacgt ttgagacaat
    24841 tgacgtgaca gcagaggacg attctcgaaa aggactgaga cattactttt tacgtgtacc
    24901 gatacgaaag aaagatgact acttgagacg tctcacgcat atagaagatt ttcatggtct
    24961 gattttcttt aatcaactga ctgaattagg cgtaatggaa gaaaaattgc tgtatagagg
    25021 tgtaccggta ggaagtctgg cctctgatca gaacaagcta ctccgaaaag cagcgctaga
    25081 acaattcaaa aagaaaatgg tcagagcact tttgaccaca gatgtggcgg ctagaggttt
    25141 agatattaca ggtcttcctt atgtagtgaa tgcagaagtt ccgttaagtg aagaagctta
    25201 tttgcatcgt tcgggaagaa ctggtcgaat gggtaatgaa ggaatagtaa ttacactcgt
    25261 acaagataat aatctaagag atttaaaacg gcttttacgt ccatcaaata taacacttga
    25321 agagattttt ttatacggag gtaagctgca aactgagcag ccaaccaaag aagatgcagc
    25381 tcctgcagta aataaaaagt atgcgaaaac tttttctgga aaggagcaga acccagaata
    25441 taacaaagaa aaaataagaa aaaaagttaa aaataaatca aaaaaagaga gaaataaagg
    25501 agcaaggcgc aaataagcgc ctttttatgc tatgattatg aaaacgggaa caaaattggt
    25561 tgccagagga ggggttatgt gaaagaacgt caagaagaga tcttgcagct attgacagaa
    25621 aaccaacaag gattgacagc aagtgatgta gctgagcgct taactatcga tcgaagcaat
    25681 gcaagccgat atttaagtga actatacaag gcccatcata ttgtaaaaac cgctggccga
    25741 ccagtcgttt actctttgcc aacggaaaag tcaaaatctg atgaagtgca tgtcgactct
    25801 tctacgcaag tgacttttga aacactcgtt ggagaaaatg attctttaaa agtcagcata
    25861 cagcaggcaa aagctgcgat tctttatcca ccgcgggggt tgcatacgat tatttttgga
    25921 gaaactggta cagggaagtc gatgtttgct gaatgtatgt atcattttgc aatcgattca
    25981 gagatgcttt ctgcagatgc gccatttgtt tctttcaatt gtgcggatta tgctcaaaat
    26041 ccgcaactgc tctttggaca catctttggg atcaaaaaag gggcttacac aggagcagct
    26101 caagatagtc ccggtctaat agccaaagca gacggcggta tattgttttt agatgagatc
    26161 caccgtttac cgccggaagg gcaggaaatg ctgtttactt tcattgataa aggtatttac
    26221 cgtccgcttg gagaaagtgc acaagtacat gaagcatctg ttcagatcat tggggcgacg
    26281 actgaatctt cagaaagttt cttatctacg ttcaatcgac ggattcctat ggcaatcacc
    26341 ttgccgaatc ttgcttcacg ttcactggat gagcgttatg aaatcatctc tctttttatc
    26401 aaacaagaag cgaatcgttt gaatcaacgt atcgatatcg aaaaagaagc gattcttgcc
    26461 ttcatgcttt atgatgcgga aggaaatatc ggtcaagtga aacgagactt gaaactagtc
    26521 tgtgcaaaat cttttttaca ttaccgtact catcatgaga aaaagctaat cattcgaaaa
    26581 gaagaacttt cccttcaagt acaaaaagga ttactgaaaa tcaaggaaat accggaacgt
    26641 ctcgaccgat tcatggacag caaaagtcag tatttgacat ttgaaccagg atttgctgat
    26701 gttgtctggt cacaagatcc agaacgaaac atgcaggtct ataacgatat tgaggaaaaa
    26761 gtcacttctc tttccgaaac cggtgtggag aacattgatc tagagaccct catttcaaaa
    26821 gatgtggatg cttatttcca gacatatgtc aaagaattga ctaaaagttc gattcccaaa
    26881 gatcttttgc cagatgaaat ctggcggtta accaatcaat tgtatgatgt ggctgaaaaa
    26941 cggttagacc gtatttataa tgaaaaagct cgttttgctt ttgctttgca tctgcaaagt
    27001 acattggatc gtgtgagaga aggtcatatg atcgtccacc cagatttgaa taacgtacgg
    27061 aaaaatttta agagggaatt ccaggtagca atcgatcttt caacgatcat tgaagaagaa
    27121 gaacaagttg agattccttt tgatgaaatc ggctttatta gtatgtttct ttctatcaat
    27181 ctaggagaaa gtgagcctgt taaagaaaac aaagtagatg ttctcatatt gatgcacggc
    27241 agacagactg cttcaagcat gttggagatg gcacaagaac tattaggaac aaaagtaggg
    27301 aaagctttta atatgccact ggttatggaa gttcaaacaa tgtatgaaga agtgttacaa
    27361 tatgtcaaaa actatcaaga gcatttgacg aatggcctac tcttgttaac agatatggga
    27421 tcattaaaca ccttcagtga tttgatttat gaagagactg gcattcggac gaaagctatc
    27481 acgatgacaa gtaccatgat tgtgatagaa gcgatacgga tggcaagtgt tggaagaagc
    27541 ttagaagata tttatcaaaa tatccagctt tcctttgaat cgatcgtacg tgatcagttc
    27601 aaagcggaaa agtctgaaac tttacagcgt aaaaaagccg taatcgtgac atgctttact
    27661 ggtgaaggag tagctgccaa attatatcag cgtattatgc cagtgattaa tcaaaataag
    27721 gtagaactga tccagatgca gtttttagaa cgtgaatcat tcaaaaaaca catcgatgcc
    27781 ttattggaag aatatgagat caaagcaatt gcgggaacag ttgatgttga ttatcaaaat
    27841 atcccatttt tttcagccta tgacgttttt gatgatgaaa aactaaatat tttaaagcgt
    27901 atagctgatg atgagatccc gacagctaaa attgtcgatt cacttgctgg atcattaagc
    27961 gatgtctttt ctattgatga actgatccgt tcattgcaaa agacagttca gcagatccag
    28021 accgatttgt cgattatcgt ggaacctgga gtagatactg gaatcgttat ccatttagcc
    28081 ttcatgttag acggattgat caaacaagaa agccatcggc aatttgagaa actgtctatt
    28141 ttctcaaaaa tttatcgttt ggaaatggat atcgttcggg caaacttgat ggcactagaa
    28201 cgaaaatatc aggtaaaaat tccagaagat gatatcgcat ttcttacaca aatgtttctt
    28261 gaaaataaaa taaaaaaatg aacacactaa tcatactgtt tacacactaa acatctgtgt
    28321 gcgcactata aaaaagagct aaaaagcatg aaaacgctat ttcagctctt ttttttattg
    28381 gcatgcttct tgcgttatat aaaagtgtaa aaaaataaaa ggagtgttaa aaatggcaaa
    28441 aaaaacaatc atgttagttt gttcagcagg gatgagtacg agtttattgg tcacaaaaat
    28501 gcaaaaagca gcagaagaaa aagggatgga agccgatatt tttgcggtct ctgcctcaga
    28561 tgcagataat aatttagaat ctaagaatgt cgatgtatta ttattaggtc ctcaagtacg
    28621 tttcatgaaa gctcaatttg aacaaaaact tgcgcctaaa ggtattccat tagatgtcat
    28681 caatatgcaa gactatggca tgatgaacgg agaaaaagtt cttgcacaag cagaaaatct
    28741 aatgaaataa atcaagtatg cagttggggg tataacaacg tggaagatca acaaaatcta
    28801 gaagccatca tggggttaat catgtttggc ggtaatgcaa agagcgatgc aatggaagcg
    28861 attgcggctg ctaaaatcgg tgattttgag ttagcggata ggaaaatcgc tgatgcagaa
    28921 gaatcactta tacaagcgca tcatgcacaa acagacatgt tgacacaaga agcacaagga
    28981 aatcatatgc aagtgacatt attgactgtt cacagtcaag atcacttaat gacatctatt
    29041 gcgtttactg atttagcaaa agaaatcatc gaactttatc gtcgcgtata cagcgaataa
    29101 atacaacttc ttttttgaac ggaagaagaa aaatttttga acggattttt tattttgtta
    29161 taaggagaac tctctttata taaaaaactt ataggaggaa gtaaaaaatg aacggactat
    29221 caagttggat ggagaaatac atcctgccgg ttgccgcaaa aatcggagcg caaaaacatt
    29281 tagttgcttt gcgtgatgct tttatcggca cactgcccgc aacaatggcg ggatcagtcg
    29341 ctgtaatgat caatgcaatt attcgtgatt taccgcaaca gtttaacagt acctatgctg
    29401 acagtttagc agcagacaaa ggaatctttg ccgtattgaa tgtcatcatc ggaatcaatg
    29461 gttttgtctg gaacggtaca ctagcaatcg ccggattgat ctttgccttt tcttgggggt
    29521 ataatcttgc ccgagcttat ggagtaaatg atctagctgg ggggatcgtt ggtttagcta
    29581 ccttgattca aggtatttcc tttggtccag cattatcagc agcattagat gtgaatgttc
    29641 cttcaaatgt cgcagatgca atcaatagtt ctgagattgg tgcaacgatt gcagatggca
    29701 cactgtcagt tggattatgg ggttggctaa aactagatca tttgaatgga aacgcttatt
    29761 ttacagttat gatcatggga gctatttcag tcattatttt ttgtaaattg atgcttgcca
    29821 acattacgat caaaatgcct gattcagtac caccagcagt atctaaagca tttgcagcca
    29881 ttttaccagc gacgatcgct ttatacgtca ttgctattat caattatatc gtaggtcgtg
    29941 tagctgatgg gcaactgatc attgatttgg tgcaaaaata tattgcagaa ccattcctag
    30001 gattatctca agggttggga gcagtattga tcgtcacagt atttgttcaa gtattctggt
    30061 tctttggtat tcatggacca aatgtattgg caccagtgct agagggaatc tggggacaag
    30121 cacaactaat caatattgat attttccaaa aaggttataa cggaaaaact ggtacaccag
    30181 ccgtgttaga tgcaatcgaa gatggaaaag cttatatgtg ggtccgcgga tcgttcgatg
    30241 cttttgcatg gtttggcgga tcaggcggaa cgatcgtctt actgatagct atcttgattt
    30301 tctctaaacg tgcagattat ctaacagtag ctaaattatc cattgggcct ggtatcttca
    30361 atatcaacga accagtgatg tttggtctgc caatcgtatt gaatgcaatc atgtttatcc
    30421 cattcttgat tgctccagta gtagcaacag caattggatg gacagctact tatcttggcc
    30481 tagtcgctcc ggtatcgcag caagtcacat gggtagtacc gccgttctta ttgtcattct
    30541 tagcaacagg agcggattgg cgggcaccga tcgtaacatt agtatgtatg gtcgtgactt
    30601 tcttgatttg ggcaccattt gtcatctctg caaacaaaat ggaccctgcc gcaggcgaac
    30661 aacaataaga tgaacgtgag gagcgggacg ctttaagttt cgttcctcat aatgaatttt
    30721 cgaggaggaa ctgaaatgat tacaattgcc agtgcaatgc aacaagatga aatcaaagaa
    30781 ttgttagaag gatacaatga agaaggaatg acttttacat ttaaagaaaa acaaggaatc
    30841 aagctgttct ttgaaacaag tgcagaagat ccggaagttg cggctaaaaa agcaaaggaa
    30901 ctaatcaaag cacagccttg gggaacagtc ctttattttc aatcaacagc tgtttagaaa
    30961 atggtttaaa aagataggat ggttgagatg ataaaaaacg gtttattttt atgtagtatt
    31021 ggttctttga tcgtgttgta tagtttgaat ccacattcgc aaaggtatga tttgtatagt
    31081 atggtcacag gtctttttct aacaggatta ggaatctatt tcttcatgaa ggggaaaaaa
    31141 agagaagaag caaaggagaa aaataactaa tggaaatcac gaaaaaatta aatattccag
    31201 catcgttttt ttacgataaa gttatggact ctgtcctttt cgatgtacgt aaagcgaccg
    31261 gcaaatcagt aactagaaaa cagctgaaaa atttggagta cgtcaaacaa ttctcgcaaa
    31321 ataaccgggc tcgcataaaa atcgaagaag tcattgaaaa ccagtcttat aagtttcgta
    31381 catcgacaac gaagaatgaa ttcgtcgttc attatcaaat caaaccattg gacgattcga
    31441 cctgtgaggt tcattacaca gaaaaaatgg agtcttatgg catgcttcaa aagttgaatg
    31501 atatgctttt aggaacaatc ttgatgtatt ttaaacggag acgtttcaaa aaaatgctag
    31561 atatgatcga agcaacgtat taaatcaatg aaacatggta ctatttagtt attgaaaaat
    31621 gaagtaagct agaaggaatt tttcatagga agatagatct ttcctgcgaa tcctcactag
    31681 cttttttgta ggaaggggca gcaaaatgac aaacaaagta agagagctag gtgtatcttc
    31741 gattgaggaa atgtttgatt tagccactta tgcatttaat ggggcagata cacctgaaag
    31801 gcgagaacgt tttcgtacac ttgcggagaa ttcttggaat tacggatttt ttgatgagga
    31861 ggatcggcta acaagtcaag tcatggcgac cccttttcca gtgaattttt acggacaaga
    31921 gtacctaatg gccggcattg gctatgttgc ttcttatcca gaagcaaggg gacaaggcgg
    31981 gatcaatcag atcatggaaa agatccttga agattgccgt gatcgtaaag taagcttatc
    32041 ttatctggcg ccattctcct atcccttcta tcggcgctat ggctatgaac aatcatttga
    32101 taaaatcagt tatcaactaa acagtcgtga tgtgccgttt atcaaaaaaa aatctggaaa
    32161 aataaagcgt atggattttg aagatgcaaa agctgcaatc agaagaattt atgaacagat
    32221 gccggaaaac caacgagctg gtctgaagcg cgaagactgg tggtatacgt ataaattcaa
    32281 gcaaaaaggc aattaccagt ttgctttgca ttttgatgat aatgaggaag caacgggata
    32341 tatcgtttat cagcttgcaa cgtcttcctt tattattgcg gagtggggat ttttggatca
    32401 tgatgctttt tgcagccttg ttcgcttcgt aagttctcac aatggtgctt ttgaaacatt
    32461 tgaatattca tgcggctacg gcggtaatga tcaaaattat ctattagaaa atccatttgc
    32521 ttctgtaatg atcacacctt atatgatggc gaggatcgta gatatttctt tgtttttgga
    32581 aaattatcca tttaaaaaga gacaagctgc atttgcccta caaatagaag aagatcatta
    32641 tgcgaaatgg aatgaaggaa tcgtggaagt aacaatcgaa aatggaaaaa atcatctctc
    32701 aaaagtagaa caaacaaaac tcccaatcgt tcgaggaagt atccaacata tcacgcagct
    32761 attattaggg tatcgtaaag tggaagagtt ggtattccaa gaaagaatac aggtgacaaa
    32821 agaatatgaa gaaacgttaa gctcaatctt gccacagcaa cagccaattc ttaatgacta
    32881 tttttgataa aaaacgggtg atgacaatat ttttgtcacc acccttttgt ttttcatttg
    32941 gcatttgaag gaaaatgtta aagtaaatcc tcttttttcg taaatttgaa ttttttgttt
    33001 tcttttgttt tagggttttc ttttttgtgg tcttggattg cttctttcgt aatcttttcc
    33061 cgattttttt tagatgaatc taagcgttct gcgtatactt tttcttttgg cttatcattc
    33121 attggaaagg acctccctac attatttcat tctacctcta gtttatcatc tgttaaggac
    33181 tagtggaaac gttttgccta acttataagc tgattttttt agcagataag cttgcaagta
    33241 aaaaacaaac aagctacaat tgatttattg aaattgttcc tgtagctcag taggatagag
    33301 caatcgcctc ctaagcgata ggtcgctggt tcaattccag tcaggaacgt ataaataacc
    33361 cctgtatcct ttgcggtaca ggggtttgtt ttgtatttgt gccaaaaatg tgccaaacta
    33421 gttatcattt ttatataaac tatcgaacac agtattgatt ttttctttat cctgatcttc
    33481 aagctctcga acaatatgaa gat
//
LOCUS       DAZUKC010000026        32168 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000026 DAZUKC010000000
VERSION     DAZUKC010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.027"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(105..485)
                     /gene="yajC"
                     /locus_tag="ACG80T_001156"
     CDS             complement(105..485)
                     /gene="yajC"
                     /locus_tag="ACG80T_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFS4840822.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(582..1727)
                     /gene="tgt"
                     /locus_tag="ACG80T_001157"
     CDS             complement(582..1727)
                     /gene="tgt"
                     /locus_tag="ACG80T_001157"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFS4840823.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1945..2643
                     /locus_tag="ACG80T_001158"
     CDS             1945..2643
                     /locus_tag="ACG80T_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFS4840824.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2811..5450)
                     /locus_tag="ACG80T_001159"
     CDS             complement(2811..5450)
                     /locus_tag="ACG80T_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS4840825.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6069..7100)
                     /gene="queA"
                     /locus_tag="ACG80T_001160"
     CDS             complement(6069..7100)
                     /gene="queA"
                     /locus_tag="ACG80T_001160"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFS4840826.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7293..8468
                     /locus_tag="ACG80T_001161"
     CDS             7293..8468
                     /locus_tag="ACG80T_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS4840827.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8472..9113
                     /locus_tag="ACG80T_001162"
     CDS             8472..9113
                     /locus_tag="ACG80T_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4840828.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9110..10027
                     /locus_tag="ACG80T_001163"
     CDS             9110..10027
                     /locus_tag="ACG80T_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4840829.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10024..10695
                     /locus_tag="ACG80T_001164"
     CDS             10024..10695
                     /locus_tag="ACG80T_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4840830.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11236..11724)
                     /locus_tag="ACG80T_001165"
     CDS             complement(11236..11724)
                     /locus_tag="ACG80T_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFS4840831.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11925..12191)
                     /locus_tag="ACG80T_001166"
     CDS             complement(11925..12191)
                     /locus_tag="ACG80T_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFS4840832.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12191..12454)
                     /locus_tag="ACG80T_001167"
     CDS             complement(12191..12454)
                     /locus_tag="ACG80T_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS4840833.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            12975..13115
                     /locus_tag="ACG80T_001168"
                     /pseudo
     CDS             12975..13115
                     /locus_tag="ACG80T_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(13257..15080)
                     /locus_tag="ACG80T_001169"
     CDS             complement(13257..15080)
                     /locus_tag="ACG80T_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS4840834.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15678..18383)
                     /locus_tag="ACG80T_001170"
     CDS             complement(15678..18383)
                     /locus_tag="ACG80T_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFS4840835.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(18689..19636)
                     /locus_tag="ACG80T_001171"
     CDS             complement(18689..19636)
                     /locus_tag="ACG80T_001171"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFS4840836.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            19826..20095
                     /gene="rpsN"
                     /locus_tag="ACG80T_001172"
     CDS             19826..20095
                     /gene="rpsN"
                     /locus_tag="ACG80T_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFS4840837.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(20271..20633)
                     /locus_tag="ACG80T_001173"
     CDS             complement(20271..20633)
                     /locus_tag="ACG80T_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS4840838.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(20644..21765)
                     /gene="alr"
                     /locus_tag="ACG80T_001174"
     CDS             complement(20644..21765)
                     /gene="alr"
                     /locus_tag="ACG80T_001174"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFS4840839.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(21781..22131)
                     /gene="acpS"
                     /locus_tag="ACG80T_001175"
     CDS             complement(21781..22131)
                     /gene="acpS"
                     /locus_tag="ACG80T_001175"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFS4840840.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(22261..23772)
                     /locus_tag="ACG80T_001176"
     CDS             complement(22261..23772)
                     /locus_tag="ACG80T_001176"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS4840841.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(24094..25446)
                     /gene="murF"
                     /locus_tag="ACG80T_001177"
     CDS             complement(24094..25446)
                     /gene="murF"
                     /locus_tag="ACG80T_001177"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFS4840842.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(25599..26675)
                     /locus_tag="ACG80T_001178"
     CDS             complement(25599..26675)
                     /locus_tag="ACG80T_001178"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFS4840843.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            26871..28616
                     /locus_tag="ACG80T_001179"
     CDS             26871..28616
                     /locus_tag="ACG80T_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFS4840844.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(28896..29333)
                     /locus_tag="ACG80T_001180"
     CDS             complement(28896..29333)
                     /locus_tag="ACG80T_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFS4840845.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(29317..30690)
                     /locus_tag="ACG80T_001181"
     CDS             complement(29317..30690)
                     /locus_tag="ACG80T_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840846.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(31012..31500)
                     /locus_tag="ACG80T_001182"
     CDS             complement(31012..31500)
                     /locus_tag="ACG80T_001182"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4840847.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(31522..32124)
                     /locus_tag="ACG80T_001183"
     CDS             complement(31522..32124)
                     /locus_tag="ACG80T_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFS4840848.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         9195 a         6878 c         5940 g        10155 t
ORIGIN      
        1 aatattgagc aatatatccc atgctcttat ctctttcttt tttgaataaa aaatgaggta
       61 ggacatcttt ctgccctacc tcgtctttaa ataagctttg atacttattt ttcttccttc
      121 tcttctgtct cttccagcgt ttctgttgtt tcagttttta cttcaactgt ctcagctggt
      181 tcttctgttg tttcaacagt agtcgttgca tcatttgtta cgacagtccc tggtttgacc
      241 gttttgattg ctgcacgatc gtactcaagg aagatccctt cgcagtctaa aacaactgtc
      301 cgtttgtcat tgtcgatttc tgaaacaact ccatgtaatc cgccgatcgt tacgactgaa
      361 tcacctggtt tcatgctatc taacaagttt tgacgttctt gctgttgttt cttttgcgaa
      421 cgtgacataa aaaaccacat tgctaatagc gcgataaaca tcacaagcat tggtaatgtt
      481 cccatttttt tcacctcact aattctgtat ctacaaaaaa cactttagca gaaaaatgct
      541 gataacacta gttttttact taatttttaa aagattttcc tttagaagct ttttgcgttt
      601 tctttattga acccgtattc ttcaaaaaag gcttctctaa attcaagcaa attatcatcc
      661 atgattgctt gacgtacttg tttcattaga ttcaacaaga agtataaatt gtggtaagaa
      721 gtcaaacgaa tcccaaatgt ttcatcacat ttgatcaaat ggcggatata ggcacgtgta
      781 tagtttttac atgtataaca atcacatttc tcatcaattg gacggaaatc atgggcatat
      841 ttcgcatttt tcactactag acggccttga gaagtcatac atgtgccatt tcgagcgatt
      901 cgagtaggca agacacagtc aaacatgtct actccacgga taacaccatc gatcaatgaa
      961 tcagcagcac cgacacccat caagtaacgt ggtttgtttt cggggatcaa cggtgtagtg
     1021 aattctaata cacgattcat ttctgattta ggttccccga cagataaccc gccgattgag
     1081 taaccaggga aatccatccc gactagatct ttcgcgcttt gacgacgaag atcttcaaac
     1141 cctgctcctt ggatgattcc aaataatcct tgacggtctg gattagcatg agctttcaat
     1201 cctcgttctg cccatcgaga cgtacgttct actgattttt tcacgtagtc gtaactttca
     1261 tcgaatggtg gacattcgtc aaaactcatc atgatatcag agcccaattt attttggatg
     1321 ttgattgctt tttctggtga taaaaacata cgcgaaccgt tcaagtgatt tttgaaatgc
     1381 acgccttctt cttcgatatt ccgcatatca cttaatgaaa aaacttgaaa gccgcctgaa
     1441 tctgttaaaa tcggttgatc ccagttcata aatttatgca gtccgccagc ttcttctact
     1501 aagtcttcac cagggcgtag ccataaatga tatgtattac ttaaaataac gccagcaccc
     1561 atttctttca actcttctgg ggacattgtt tttactgtag ctaatgttcc tactggcatg
     1621 aacattggtg tggggaaagt tccgtgtggc gtgatcaatt cccctaaacg cgcgccagta
     1681 tgtttttctt tcttgattaa acgatagcga atggcaggtt gcgtcattca tttccctact
     1741 ttctatataa aatatcttga caactcccac catcataatc gtttcaagtg ctttttgcaa
     1801 gttccctcgg cttccttcta ctttatttaa tattcttttt tttatccttt ttttatgaat
     1861 aacaaaaaaa agaaaaacat tatgacagaa atattttttg atacatggta tattggtgtt
     1921 attaaagaaa aggagggaaa aatcatgcaa tcttctgcag aattaaaacg ccaagcaaaa
     1981 gagagtctaa aaggacgttg gggacaagct gttttactaa atctaatccc tacattgatt
     2041 ggtattgcta ctattcttct tattatgatt cctattatct tgtttgccac caccatatat
     2101 gatggatcag cgagcgtctc agaagcgaca acttcaggag gcggtagttc aggaggcggt
     2161 ggcggaatta tttccagcat cattagcgct ttgttcatga gcggtatttc ttggacttat
     2221 cttgatattt tacgtggaca aaaagatcag atcgaaccat tcaaagatgc atttcgtgga
     2281 ttcaaaggtt tgtttatcgg cggagtgatc agcttagctg tgctgatctc gatctttaca
     2341 acattatggg gtttgctatt agttattcct ggaatcatta aaagctattc ttattctcaa
     2401 agctatttta tttactacga tatcgtccaa gaaacaggac aaacacctaa agtactggat
     2461 acgatcactg caagtcgaaa attgatggat ggttataaag gacaactttt ctggttagat
     2521 ctttcattta tcggctggca tatcttagca ttagcaactg ctggtattgg ttatctatgg
     2581 ctcagcccat acatttctgc aacaaaagca gcattttatg aacaattgcc aaaagatatt
     2641 taaaagagag tgtgacttgt gaccgagggg gtggcaggag aacaccgttt atcgagaggc
     2701 agtaaaagaa acattcagcc cattagcaaa aaggaggtgt gacaacattc atgtcacacc
     2761 tcctttttgt atagttccag taaaaagaaa atcaacttag aaacagttaa tcattcctct
     2821 tcttctgctt ccggtaatcg atggaacttg atcagttcat tcaccgcgat tggtacgata
     2881 gataaaatcc ctaccaagaa ccagtgattc aaactgatgt gtactaggcc gaatagttct
     2941 tgcgtaaatg gcagcaaagc aacagccgtt gtaatagcta atgccagtaa cgccatttct
     3001 accaaagctt tattggtcgc taaattgact ttgaagatcg attggctgct gcgtacattg
     3061 aacacatgca gaatcgatga ataagctaag atcaagaatg caacagtttg accgacatgc
     3121 tgacttgcgt ctacccatgg gctgatagta tcgacataac tacctaaata ataaccaaac
     3181 aaggttacga tggtgaagac ccctgcattt atggcaattt tttgcaataa acctcgagca
     3241 aagatccctt catttttcgg aatgggtggc tgatccatga tgccgtgttc cgctggctct
     3301 cttcctaaag caaatcctgg aagtccatca gcaactacat tgacaaacag tagttgcaca
     3361 gctgtaaatg gtgctcccca tcctaacagc atcgcaatca aaacaataaa gatctccgaa
     3421 atattacagc ttagcaaaaa gttgatagct ttacgaatat tctgataaac cgaacgtcct
     3481 tgactgacag cgtctacgat cgtcgcaaag ttgtcatccg tcaaaatcat atctgcggca
     3541 ctttgcgcca catctgttcc tgtgatcccc atggcacacc caacgttact tgctttcaac
     3601 gctggtgcat cattcacacc atccccagtc atcgctacga ctgcacccgt acgttgccaa
     3661 gatttgacga tccggatctt gtcttctggc gttacccgag catagacaga gagagctttg
     3721 acacgcgcat ctaattcttc gtctgataat tgatgcagtt ctgatcctga aagtgcttct
     3781 gacggatcat tcaagatacc taattctttc gctatggcac tggcagtcac cacatgatct
     3841 ccagtgatca tgactgtttt gatgccagcc tttttcgctc gcttgattgc tcctttactt
     3901 tctggacgtg gtggatcgat cattccgatc agaccaatca aacgtaagtc tttttccagt
     3961 gcttcagaag aaatatcttg cggttcttcg tcataagtag cataaccaac agcaatcact
     4021 ctcagtgccc gtttaccaaa gcgatcatta acgatcgccg cttgttctac atcgcctgaa
     4081 ctaaagcgcg gagcaagaac atcgaatgct cctttggtga ttgagatgta tttttcgccc
     4141 atttggtgaa ctgttgtcat catttttcgt tcagaatcaa aaggaagctc gtttactcgt
     4201 ggatattttt cttctaattc tgcctttgta tgataattct cttcaacagc ccttacgatg
     4261 gctacttctg ttggattacc tttgataaca aggtcgtcat cttcttgctc gaccgtcaca
     4321 tctgtacata gtgcagccat tttcaatatt tccatcgcgc tgttcgtcat tgaatcttcg
     4381 atattcgtta cttcatcttc tttgctccat acccggcgga cgcgcatttc attttgtgtc
     4441 agcgttcctg ttttgtctga gcagataaca ttagcggttc ctagcgtttc aactgctggc
     4501 aactgtcgga tgatcgcatg tttcctcgcc atcttctgca caccgtatgc aagtgtcaac
     4561 gtaacaataa cagtcaaggt ttccggtaca gctgcaacag ccaaggaaat cgaagtcatg
     4621 aacatctcaa ggagtggttc tccttgcagt tcaccaatca caaatactaa agccgcagcc
     4681 gctaaagcga tcatactgat tcgtttgcct agctgattca atcggacctg taaaggtgtt
     4741 ttttgcatag tattttcgtt caaaagatcc gcgatcttac ccatttctgt agccatgcca
     4801 atggcagtga cgatcgcttt cccacgacct gcagcaaccg tacatccttt aaataccata
     4861 ttaagttggt cacctaatga ctcatcttct tcagcaataa atgctgcatc tttttcaact
     4921 gcttcacttt ctccagtcaa cgccgattct tctacacgta attgtgatgc ttcgatcaaa
     4981 cgggcatcgg cagaaatagc atcaccggac tctaaaacaa gaatatcacc cactaccagt
     5041 tgctctgact caacagtggt cactttgccg tctcgtatga cagttgccgt ttgtttattc
     5101 atatcttgca aggattccag tgctttttct gcgtttcctt cttggacaat cgccaatacc
     5161 gagttgatca caacgatagc gatgatcagc agtccttcaa ataaatcccc atgttctgta
     5221 gcaaatgcgg tataaaagga aatcaccgct gctacaagaa ggatgatcgt tgtaaaatcg
     5281 gacaagcttc gaaagaattt tttgatcatc gattctttcg gcgcctcatc gaatttattc
     5341 aatccctgct tttttcgccg ttcatccact tcttgtgaag acaacccagt atctggatct
     5401 gcttctgtta ccttctttac ttcttctatt gtctgcttgt attcttccat cctattcctc
     5461 ctttaaaatc ttcgtacaaa aaaaggtact tattttacta taacatcctt tttttattta
     5521 cttcaaataa agggcttatc gattatttgt taagaaagcg taaaatttaa agtgcttata
     5581 tttgcaaacg ctaccaaagg atgctattat atagatgtaa ggaaggagga taccatcaaa
     5641 tagacagaag aatttctcga tagaacagtg taagcaaaca aagttgttag aagtacaggt
     5701 ttcacccaga catgcgtgta gagtacaaac ctataaatag tgaatgcgta tggatgtttg
     5761 gatcacgaag tatccgaaac aagaagtaat gcgatacgta acaattgaat aacgtaaacg
     5821 tctgaaaatt gaataggaac aagagatgtt tcaaatgttg aagatgtaac gaaaacaaaa
     5881 tttttgttta acatgaagaa attcaacacc gtgctttgca tgtatgatgg aaagttacaa
     5941 tcatttctgt ttatttgaat ccttccgagg tacaagaaag ccgagcttat cgagagctcg
     6001 gctttttctt tgtcataaaa tctttgtaaa gcttttttgt tattcaaaac tagcggcttt
     6061 tcataacctt atttcacgaa catcgcatct ccaaaactaa agaaacgata gcgctcttca
     6121 atcgcatgct ggtaagcaga taaagtcaat tctcttcctg caaaggcact aactaacatg
     6181 actaaagtcg attttggcaa atggaaatta gttgagaaag cttctaccac tttgaattca
     6241 tagccaggcg taatgaaaat atcagtccat ccgctgtctg ctttgatttc tccattaaat
     6301 ttcgttccaa tcgtttctaa tgttcgaatt gaagttgttc cgacagccac gattttcccg
     6361 ccatttttac gtacttggtt caatcgctca gccgcttctt ccgtcaaacg gtagaattca
     6421 ctatgcatat ggtgttcttc aattttatct acgctgacag gtcggaatgt acctaaccct
     6481 acatgtagtg tcagatagac taattcaaca ccctttgctt cgatctcatc aagtaattct
     6541 ttcgtaaaat gcaatccagc tgttggcgct gcagcagaac cattttcttt cgcatagaca
     6601 gtttgatagc gatctgggtc ttccagacgt tctttgatat atggaggcaa tggcatttct
     6661 cccaatgatt caagaatctc tagaaaaatt ccttcatact caaagtcaat gatccgtccg
     6721 ccgtgttcca attcttcttt cactactgct tgcaatctgc catcaccaaa agagattctt
     6781 gtcccaactt tggcacgttt cgcaggtttg atcaatgtct cccacgtatc tccttgtgta
     6841 ttcgttagaa gaaggacttc taaatgcgca ccagtatctg gtttttctcc atataaacga
     6901 gcaggcagaa ctcgtgtatt attcataact agcgcatcac caggctgtaa ctcttcaatg
     6961 atttcatgga aatgtttatc ttccatctct ccagttttcc gatccaaaac aagtaaacga
     7021 gaatgatcgc ggtcttttaa tggtgtttgt gcgattaatt cttccggcaa gtcaaaatca
     7081 aaatcttctg ttgttaacat atcgtcactc actttctatc atgtgcttca tcattctatc
     7141 atgtttttcg ttctgttggc aaaatcagca ctttttcact tctatttttt gttttcctaa
     7201 ataaaaaaac taccctattt gtattttttc attgttttag gcatactagt aatagatagt
     7261 tcttcattga aaatagaaaa agaggtggca ccatgattga atttcaaaat gtgtcaaaaa
     7321 tttataaagg tggaaaagtc gctgtagagg acgtcaatct ttcatttgaa aagggtgagt
     7381 tcatttgttt tatcggtacg agcggaagcg gaaaaacgac gacgatgcgg atgatcaacc
     7441 gaatgactga tccaacacaa gggaaaattt tgattgatgg aaaatccatc caagaaattg
     7501 atccagtgga attgcgacgt cagatcggct atgttatcca aagtattgga ttgatgcctc
     7561 acatgacgat ccgtgaaaat atcactcttg tccaaaaatt gttgaaagtc agtcaagaag
     7621 aacggaataa aacagctgaa aaaatgattg atcttgtgga attgccccgt gagatgttag
     7681 atcgctaccc acatgaatta tctggtggtc agcagcagcg tattggagtc gttcgtgccc
     7741 ttgcagcgaa tcaagatatc atcttgatgg acgagccatt cggtgcgctt gatccaatca
     7801 ctcgtgattc cttgcaagac cttgtcaaag atttacaaga gcgtttggga aaaacgatcg
     7861 tatttgtaac ccatgacatg gatgaagcga ttaaactagc aagccgtatc gccatcatga
     7921 gtgaaggccg tgtgatccaa tttgatacac cagaaaatat tttacgtcat cctgcaaacg
     7981 atttcgtaga agagttgatt ggagaagatc gcctactaca agccaagcca aatgcgacaa
     8041 gagtcggcga agttatgcta aatacagcaa tcacgattac accggaaaaa tcattacaag
     8101 aagcaatcaa actgatgagg gaaaaacgtg tagatacgtt gttagttacc gataattcga
     8161 atgtattaaa aggatttatc gatgtagaaa cgttgaataa aaaacgagga aaagtatcca
     8221 gtgtcggcga cattttgaat aaacatgttt tttacgtaaa agaatccgcc tatcttcgtg
     8281 ataccttgca acgcatttta aaacgcgggc tgaaatacgt gccggtcgtt gatgaacaga
     8341 acaaagtcgt aggtatcctg actcgtgcat cattagtgga tatcgtctac gatgtgattt
     8401 ggggagaaga tcccgcaact actgatgttg cagagtcagc tactcctgaa gaatctaagg
     8461 aggtctgacc tatgcagcag ttttttcatc agcacggttc agaaattctc tcaaaaggtt
     8521 ttgaacatct ttatatttca ggacttgctt tgatcattgg tatcatcatt gctgtaccgc
     8581 ttggtgtctt actgacacgt tttccaaaag ctgccacgat tgtgattggc ttaaccagtg
     8641 ccttgcagac aatcccttct cttgctttat tggcattgat gatcccgcta tttggcgtag
     8701 gtaaattacc tgcgattatc gcactcttta tttattcatt attgccgatt ttacgtaata
     8761 cgtacattgg gatgaaaaat gtcgattgga attatcggga tgtggcaaaa ggcatgggga
     8821 tgactgagtt ccaatccatt ttttccgttg aattgcccat tgctatgcca acgatcatgg
     8881 ctggtattcg tttagctgcc gtttacgtga ttgcatgggc tacacttgcc tcttatatcg
     8941 gcgctggtgg tttaggcgat ttgattttta gtggactgaa taattaccaa ccagatctca
     9001 ttttggcagg tacgatccct gtaacaattt tagcactatt ggctgattgg cttctaggtc
     9061 tcttggaaca ccggctgaca ccgatcgcac ttagagagga gaatgaagaa tgaaacgtat
     9121 actaaaaatg accatggttc ttttctctct tcttatactt gcgagttgtt ccttccctgg
     9181 tcttgccagc aatacagatg aagacaccat ttcgatcaca ggtggaatca cttcagaagc
     9241 acagatctta gcaagtctag ttgccggaat ggttgaacat tatacagata aaaataccgc
     9301 aatcatcaac aatctggcta ctacaactat caaccatcaa gcgatgatga acggagatgc
     9361 ttcaatttca gcggctcgct acacaggaac agatttaacg actacattga accttcctcc
     9421 tgaaaaagat ccgaaaaaag cgtttgctac cgtgaaagat gaatttgaga aaagatacgg
     9481 tcaaacgtgg ttcccgtcct atagttttga aaatacgtat gtctttcttg ttcgaaaaga
     9541 caccgcacaa aaataccatc tatctaaagt cagcgatctg aaaaatgtag cggatgaatt
     9601 agtagctggt gtagatactt cttggatcaa tcgtaaagga gacggttatg acggcttcca
     9661 agaaacttat ggcttctcct tcaattcgat attacctatg cagattggac ttgtttatga
     9721 cgcagtcgaa gcaggaaaaa tggatattgt tcttggctac tctacggatg gacggatcgc
     9781 cagttatgat ctagtcatgt tggaagatga tcgtcaattc ttcccaccat atgatgctgc
     9841 tccagttgta gacaacaaga tattagctga tacgcctcat ttaaaagaag ccttaaataa
     9901 gttaggtcat accatcagta cggaaaaaat gcaggaattg aactacgaag cagataataa
     9961 tctagtggaa ccatcagtag tagcacaacg tttcttacaa gaaaaccact attttgaagg
    10021 gaagtgaagc aattggataa aatgaatgtc tttcaacaat ttttctatta tttccaggaa
    10081 aatggctcct atatctttgc acaatttatc cgtcacttcc tgatttctat ctatggtgtc
    10141 ctctttgcag cagttgtcgg gattccggtt gggatcatga tttctaggag acgtaaatta
    10201 gcaaactggg tgattcgttt ggcgaatatc atccagacga tcccttctct agccatgatc
    10261 tccattttaa tcattggatt aggcctggga gtcaatgtcg tgattgttac tgtatttttg
    10321 tattccctct taccgatcat caagaatacg tataccggga tgatccaagt agacaaaaac
    10381 atccttgatg tcggcaaagg catggggatg actgctagac aacgactatt catggtagag
    10441 ttaccactct ctgtttctgt tatcatggca ggcataagaa acgcgttagt tgttgccatc
    10501 gggataaccg cgatcggtgc attcgtcggt gctggtggtc ttggagatat cattatccga
    10561 ggaaccaatg caacagaggg cacatcgatt attttagcag gtgcattgcc aactgcatta
    10621 atggcgatca tcacggactg gcttttaggt attttggaaa gacggctaga tccagccagc
    10681 cgtacatcac gctgatgtta ttgtaaaaaa caaaagcttc tgcgaccatt tgttggtcgc
    10741 aaaggctttt ttagttctgt catttttaag gcaataatcc actctgattc ttccctcctt
    10801 atgggcaaag agttgctttt cttagctatt tatccattcc gtatactcca acttcatact
    10861 gccaacgggt tcatatctgc atccaccgac taagtttttt tcttatccgc taaggcattt
    10921 tgccaatcca ttttcccatc actcgtcatg gaattcatac cacatgatct ctaaatactc
    10981 gtctgcttgt tctttgaatt gggcatcaat tcttacatcc tttggcgtgg ctttcattta
    11041 agccttttcc atcagtcctc gattgttcga tgccttcagc tgcatcatca aaactggtga
    11101 ctgccgtatc ggtaacttcg atcatctttt atacaatcgg gaacttgcca tcctcatcct
    11161 tggctaaaaa catatctagc cattccagct tctcgccatg ggaggtttgc ggttcctaaa
    11221 accggtacac gattcttatg gactgcatcc gtcgcaacta gacttggcga tacgatgatc
    11281 cttcaccaga agagcacccc cgtaaaccaa ttgatcgata tattgccaat aagaaaagac
    11341 attggcgtaa aagatatcga tccctcgtga tgcattgccg ttgatactgc tattcatggc
    11401 cgaaagcgcc acgactttaa cttcttccgt ttgagtgaca ttcatgttcg gcaatgtcgc
    11461 tttggatacc cgctctgcta aaggaacgac actggtattg tatttcgtat ctgtatcttt
    11521 tccaaaagac catgccaacg aatcctctgg gaaccaataa gacgactccg gttgattgtc
    11581 cattgctttt ttcaacatac ttttggctgt ttctcgataa acgacatttc caattgtctt
    11641 cgcctcactt tggcgataat cgatggtcgt tgccactgcc aacactaaca acgatgctcc
    11701 aattttactg attttccttg tcacacttct tcctccttgt tttacataaa cccgtcaaca
    11761 ttccattcct aaactcaaac aatgaccctt caaaggtgga gatataggaa caaattaaag
    11821 aaatcatgca ataaatccaa gaaggttggt tttagaacaa aaaaagagtc tcctttttgg
    11881 ggagactctt tgttaactat ctaaaaaggt tggctcttct cacttcactc ataatgtgtc
    11941 caggcagaat aaatttctac aactttattt tcctcatcaa ctttgtaaac cactcgatgc
    12001 tgtctattca gcctgcgtga gtatctgcct tcatgtgtag gtctcagctt ttcaaaagac
    12061 tgcgtgggca tatatggatc ttccttcaat gtctgaatca cttcttcaaa ttgtttcttt
    12121 aaattcgttt gcttgatttt tcttaaatcc actttcgcag agtttttgat tgcgactgta
    12181 tagttactca tcaaagagct tcccaatcaa tatcatcgat attcgtaaag ctactgtcgt
    12241 ctttttcgcg gtctcgtact ttatctaaag tacctgtttg ctcaagaaaa agggtttctt
    12301 gaatcgctct ccagtcttcc agcccaatca atacagcatt gtttccacta cgatcactaa
    12361 taatctcgat ttcagtatga ttttcattta cctctttcaa taactgataa aaatttttac
    12421 gagcagtgct aggattcatc gttgtattag ccattgtctc tcctcctttt tctgatataa
    12481 ttatagcgta cttgattaag tacgtcaaaa aaatacaata aagcacttaa attcagcaaa
    12541 cttttcctct tttttacata gagaaaaaga ccccttttta gggaggatct ttgtccacgc
    12601 tcacttctta ctcctttacg actagcggta gccaaacgta aagtaattgg taacaacgta
    12661 tcatctttag caaaggtcga atccaaagcc tgtgagtcta tgcaacaaca ccaatacacg
    12721 aaggttcctc gttgcagtgt gacttgattg atatcttgaa taacctgagg atacgcacga
    12781 aagatttgta acggttattc aaaactgatt ttggccgtca agtcttctac tgcctcaaaa
    12841 atgattgcct ctttcgtctc ctgtcattca tgttcactat cgatttgata cttgcttaga
    12901 ccgttgggtt tgtgaatcac caattaaaaa tggcactgag ttatggtaac aaaaaatggt
    12961 cacttgacga ggctgagatt cctacttatg aagacacatg gcacttaaga gtcgaggtaa
    13021 aggaagaagc tgaaaatata ggaggctgta cattatttgg caaaggattc ggaaattatg
    13081 atcaagacat caaaatcaaa atgttttaca gctaaatagg aagaataata acatttcata
    13141 cgctgttccc atctttaatg gcttatagag ggcaccttta gctgagaata agacaaaaca
    13201 gaccctctag cttcaattaa gagctagagg gtctgttttt gattttaaaa cttggtttat
    13261 ttcttcaaat ggtagctata ggtgctgaca acgatatttt cacgccaaga caatcgcaat
    13321 cttaaccgca aacttgtttg gttatggaga atattctgcc agcgtctgtt tggtacaaat
    13381 tgaggaatca acacagtagt tgtatagttt tgtttcgctg catttttact tacgatatct
    13441 acataacgga taattggatt ttcaatcgag cgataagaag agtggacgat tgaaaaacgg
    13501 acatctggga aatgcttttt aaattctgct tcgatttctt tttctttttc gacatcttcg
    13561 tctagcgaaa catgcatcgc aacgacataa tcaccgatcg aacgtgcgta attcaacgca
    13621 ccgatattta cccgtgtcac attcccgact aatacaagaa cggtattccc gtcataatca
    13681 tgtagttttg catcattttc taaacgaagc tgttctgcta cttttttata atgatcatgg
    13741 atcttataaa aaatgaacat aacgattggc ataatgatga agaaaggcca aatgtcccct
    13801 aaccgataga caaacaaaat cgcaatgatt gcatatgaga tgaacgcacc cgtaatattg
    13861 gcaatcgatt tgcttaacca tctttttcct tcttttttcc atttgacgac catccctgtt
    13921 tgagataaag caaatggaat gaacacgccg atagagtata acggaatcaa gcgttctgtt
    13981 gagccatgga aaataaacag caatacgatc gaacctagcg ctagtgtgat aatcccattg
    14041 gaatagccta gtcgatcccc acgatcttga tacatgtgag gcatgaattt atcttttgcc
    14101 agattgtatg caagaactgg aaaagcagaa aaaccggtgt tagcagcaac tgctaaaatc
    14161 agcgctgtag caaattgaag gacgtaatac aaaataccat gtccaaagac agctttacca
    14221 atttgagaca aaacagtgac ttctttttca ggaacgatcc cataccaata gttgataaat
    14281 gtgatcccca cgaagaagaa acctaagatc aatgccatca tcgtcaatgt agcagcggca
    14341 ttttttgctc ttggcttctt aaagaatggt acagcattac taatcgcctc caccccagtt
    14401 aaagaggaag aaccagaaga aaatgctcgt aaaatcaaag cgatagaaac acccggcaca
    14461 gccgctcctg gaagtgcagt agcttgcaat ggcacagcac ctgtcacgat attatataaa
    14521 ccaacaacga tcagtaacga gatcacaagg ataaatgtgt acaccggaaa taataggaac
    14581 gatgcagatt ccctcagccc acgaagattc agcatcatca gtagcaaaac gattgtcaca
    14641 gaaatagcga cttgatgacc gtagagtgct ggcaccgctg atgtgatcgc ttcagcacct
    14701 gctgagacag atactgctac tgtcaacatg tagtcgatca ataaagagcc accggaaatc
    14761 aagccagcat tctttccaag attctcactg ctgacgacat atgctccccc gccatgagga
    14821 taagcatgga tgatttgacg atatgatagc gttaatgaaa tcaataaaat aataacaaaa
    14881 gcggcaatgg gtagcgaata ccagatggct gcagcagaca aagcgactaa aacgacaacg
    14941 atttgttccg taccatatgc aatagatgat aacgcatcag acgacaataa tgccaaagca
    15001 gcaaatcggg ttaatttgtg ctcatcattt tcagcagatt ttaaaggctt acctactaac
    15061 aaccttttta agtaatccaa tttctaaact cctttacaca caattttact tcaagtttta
    15121 tagtcgaaaa tttatagtaa acaagatgtt atagtacgct cactgacctt aaaagtcaat
    15181 aaactttaag aaaataattg gaaaaaaaca gaaaaatatc aagcgctttt tcaaacgctt
    15241 taatacagca aaaatctaac atttttttat gggctttttc tttgaattct ataataaaag
    15301 gatagaactt gacttagaaa atcaagttct atcctttttg atatcttaac ttttggaaga
    15361 gagatgtaag gctgctttct acccatcatt cttactactc gaagcatagc gcagttgtca
    15421 caacctcttg ataaacggtg tttaaaagct tgcatctgcg gtaataagcc caaccaaatt
    15481 aaaaacataa gaagctgttt ttatttggtc gttcttatta cttgatgcag agcagctttt
    15541 ttacaacctc ttggtacacg gtgttcaatc agctgaccct cggtattagc tcaaaaaaac
    15601 ggcaaaatat gaggaactat ttttccattt ttttctctaa tactcaggtc aaaacactga
    15661 tttcacaacc tcttttttta atgaaaaata tggctctcat gcgccgcttc tttgaatttc
    15721 gtcatttttt cttcgatgaa cggatagatt gccgttccta cgattccaaa gacaacaaac
    15781 aaaccgatca tgatccagat tgctttcgta gcatttggcc aataaattcc tcctgcagat
    15841 tcccgtaaca agtttaccgc atgagtaaat ggcaagaacg gattgatcat ttggaagaat
    15901 ttccctgaga cttggatcgg atagttcccg cctccgccag agatcgacag tactaagatg
    15961 ataatagcga tccctttacc gacattatcg aataatgcag ccaagacata gacgatcatc
    16021 atgaacgcta aggcgatcaa gacagcaaat gacacgctgt agaccggatt ttggacatct
    16081 actcctagta agaaaatatt ccctaatgta acgatcaagc tttgcgcaat cgccattgtc
    16141 aaaaatgtca gcatacgagc aacgaattgt tctcgtttcg taaaattgtt tcggtctttc
    16201 tttcctaaat agtattctgt cgtagcaaca cttgaaagaa gaacagcgcc gacccacaga
    16261 catagtgcag tataaaacgg ggtgctagct gaaccgttat ttgcgattgg ataaagcgca
    16321 ttggtttgta gctcaacagg ttgtgtaaag aagtcacttt cttttgttgc gtctagtttc
    16381 aataatttga tgacttcgcc tagatcgact tcttgttcgc cttttcggat cgcgtctgct
    16441 gctttttgga taccagcttt gatatttggc cagtcattgt tgatcaagtc cgcagcagcg
    16501 ttcacagctg tttcgacttc tggcattttt tcatttactg ttgtcaatgt accggtgatt
    16561 tcatttttta gtgctggcca atcattttgg ataaaatcag cggccaatcc taatttttgc
    16621 tgaatgatcg gtaattcatt gttgtatagg tctgcccctt cattgatgcc attgacgatg
    16681 gtatccatat ttccattcag cattgtattg gcatcatgga tttcttgtcc aattgcaggc
    16741 atttcttttt gatatttttc taaaattgcc actgcattcg ttacagttgc tttcgttgag
    16801 ctgagaagcg aatcaaagtc gatctggtcc gctttgctca gtgcgccttg tgcagtccca
    16861 atcgtatcga tgacttggtc tagtagagaa gtgacagtgg aactgatttg acctgtattg
    16921 ataccagcaa tgatgccatt gatattatta acaacggcat caatttgatc tagcaattca
    16981 tcaatagcct tcgtgtcttc attttgaaca gctgtgttca aagcagatag gcggttttgc
    17041 agattgctta gcaagggttt taattgattt agctgatcaa taattggctg taatttcgta
    17101 tttccagcac ttgtttgcaa tttagtcagg aactcgacca gtttatcgat tgcattttgt
    17161 tgttgagata ttccgtcaat aaagccgttc aatacattgc tgatgttttt cttttcatca
    17221 tcagtcagct taccatcttc aatagctgtt cttacagtat ctattaacga acctgtcgtt
    17281 gttgccactt gtccaattga cttcaatgta acatctacac tgtttgtgat gcttggtatc
    17341 gcatctttca gctgagaggc acctgatttt gtttggtttg ctagatcact tgcctgattt
    17401 tctaaatttt tcacatctgg caagatatct tgtacttgtt ggatgatcgt caagccttgt
    17461 tttgcttcat cgattccttg gttcattgtc tcttggatcg aagcaaaatc gccgtcgatc
    17521 tctgctagtt gcttaccggc attttggatc tctgggattt tttcttgcaa atcaaggatg
    17581 atttttgctt gatctttcaa ctcaggcatt ttgtcattca atgcaacgac tttttcaccc
    17641 atctcatcta ctttagggat gtaatccacg aattcatttg ctttattcag tttttccttg
    17701 atttcaggta gttgactttg taattcgagg acttgttttg tgtagccgtc gatcgtgtcc
    17761 aaattttcat ctgttgataa aatcatatct ttaactttat tgatacttac taggttcgta
    17821 tcgatgtcgt aacctatctc attgaatact ttcaataaag tcgaactggc tgttttgata
    17881 aattcatttg tgatttgcga ttggatcgaa cttgctcctt tatcggtgat tttcggtgca
    17941 atcgcattga ttttttcatt taccgtatat tcgatttttg gtttcttgat atcaccacta
    18001 gtaaaactca gcagatcttc tgagaaatcc tttggcaagt aaatacctgc gtaatacttt
    18061 ccggaacgga ctccgtcttc taattgttct tttgaatcaa cgaattgcca tcctaattgt
    18121 ttatttttat gaagcgattc gatgacttgc tccccaatgg ccacttcttt accttgaaac
    18181 tctgctggct tgtctgcact gtaaacagca attggcagct ctccagtatt tccatatgga
    18241 tcccataatg ctttgatgtt aaaccacgca tatagcgaag ggatgatcat gatcgcgatg
    18301 atcagaagtg ttgcaatcgg gtttttgaaa atccgtttcc agtcaagcaa atataatcga
    18361 agcgtatttt tgattgactg catagttctc ccccttcttc ttttttcagc tgaattactt
    18421 taccatgtat ttcatacttt tttcaatcgg cttttttcaa aaaagtcgaa tttttggaca
    18481 tatcacggca attgtctcat tgtaatctct tacatattga gaaataatta aggattacag
    18541 cctttacttt atcatgaaat aaaagggaaa gataatttta tgccaaagtt tttatctatt
    18601 atacgtatac gcaaaaaagc taaaacattt tttgtctttg cccctacaaa aatgtttcag
    18661 cttttttcgt tttttattcc tgtatttctt atttttcgaa tgacaggatg attttcccag
    18721 caggaggatg tccttcaact agctgatgtg cttggatgac atgcttaagt gaagcagaca
    18781 gattcattga aataaaaaca tgaaaggctc ctttttggta ggctttgatt atgccagcaa
    18841 aggcttctgc atctagatat tcctttcttg gaacaaacga ttcataaaag ccttcctttt
    18901 tttgccgcaa atctagatct gggaggtcat ttaatgcaac atacctgccg cctggtttca
    18961 tgatctggat gccggtttct cctttgatac ttcctttcgt cgcatcgatt acaaggtctg
    19021 cctgatcagc aaattgcaga cctggatttg ttttatcata agcagcaaat gctgaagctc
    19081 ctaatttttt taccttttcc tcatttttac tggatgcact ggttaagatt cggattccct
    19141 tttcatggag aagctggatc agacttgacc cgaccgcacc tgatgctcct agtatcatta
    19201 cagtatctgt cggctggatt tccgtcaaat ggttgattag attataagcc gtaattcctg
    19261 gcgtgaccat tccggctgct tcttcccaac tcattttatc tggaattttt gctacttttg
    19321 ctgatggcaa cacgacttct tctccgtaag tcccgccgac tgcatggaca gctactcgat
    19381 cacctacatg aacagttgtg acatctgaac caatctctgt cacaatacca gcgccatcat
    19441 tacctggtac ataagggaat ttcaatgttc gtatctcttt catttctcct aaacgcaagg
    19501 caacatcata aggattcaca ctgaatgctt ttatttctac acgaacatgt ccagaaccga
    19561 cttctctggg atgagcatcg atttcttcaa aaacatcttg tgcttctccg taaccgttaa
    19621 ttgcaaatct tcgcatggtc accatctccc tttttcttta agtttacgtt tatgccttta
    19681 ttttgacaaa aaaaatgtct tttcgttttg gcgcatgatt caaaacaaaa tagttgtctt
    19741 ttttcgcgta acgtgttaaa ttatttatct aaaagcgtaa agattacgat ttacgctaat
    19801 aaaaaataat tggaggaatt tgttgatggc taaaaaatca aagattgcca aagcaaaaaa
    19861 acagcaagaa acgattgaac gctatgctgc tttacgccag gaattaaaag cgcaaaaaga
    19921 ttacgaaggc ttgcgtaaac ttcctttaga tgctcgtcca gaacgattga aaaatcgtga
    19981 tttaatcgat ggacgccctc gcggatacat gcgtaaattc gggatgtccc gtataaaatt
    20041 ccgtgaatta gcacataaag gtttgattcc aggagtaaaa aaagccagct ggtaatccaa
    20101 gtctggctgt aatgtaaagg aggagggcgc atggaccact ccttggatca tcaaaaaaga
    20161 tacgttcgct tcttctagca gaaatgctgg tactagaagc agacgtatct ttttttacgc
    20221 acagctggtc cttttttata tgtaaaggct gcatggttac ttcgctatat ttatttagta
    20281 acaggagtca gtgcttcgat acccacacta attttcaatg ctcggtcaac ttctgccata
    20341 atgggtcgat ccaaatggca gaccttttct ttcaatcttg atttatcgat cgttctgatc
    20401 tgctccaaca ggataacgga gtctctttct attcctgttc cagtagcttt gatcccaatg
    20461 tgagtcggaa gcttaggttt agccattttt gccgttatgg ccgcaacaat gatcgtcggg
    20521 ctaaaatggt tacctagatt attttgaata accaaaactg ggcgtgttcc gccttgctcc
    20581 gatcccacga ctggagaaag atcagcaaaa taaatatctc ctctttttac cataggatcc
    20641 tccttattct tgatattctc ttggtatccg ctgtcctaat ccgcaagcca cttcatagtg
    20701 gatcgtacct aattgatctg ctatatcctg catagtgatt tctttgtctg catttttgcc
    20761 gataagcgtc acttttgttc cgacaggaaa ttcttgaggc aatcggatca tcagttgatc
    20821 catacataca cgcccaatcg tttcacagta gttcccttct accaatagag aaaaaccctg
    20881 catctttcgt ggccagccat ctgcataacc gatcggtatc gttccgatcc actctgcttc
    20941 aggagtgata tatgtttctc catagccgat tccttcacct gctgggagtt ttttgacttg
    21001 aatcaattca gaaaccagct caagtgctgg ctgcaatgga taaacttccg gtagtgcgtg
    21061 cccagaggga ttaagtccgt acattgctac tccataacga atcatattgc cgattgtttc
    21121 atcatgccat aaagccgttg cactattgct gacatgtaca tatcgaggaa gttcgggaag
    21181 ttttttcagc acttctataa agcgttcttt ttgcagattc caatatgtat catctgcctg
    21241 atctgctgta gaaaaatggg taaataaacc ttcccattct aatacacgac tttctttgat
    21301 gaatgcagct gcctctttta cttcttcagg tgtacaaaat ccgatacgac ccatcccagt
    21361 gtctgctttt agatgaatcg aaagaggcgt ctctagctta cttttctcta aaacagcagc
    21421 tgctgcttgc aaccattccc gtgtacctgc agttactgat aaatcatgag caacagctac
    21481 agtcagtgcg tcgtaaggaa ccatattcaa gataaggatg ggctgagtaa atccagcttc
    21541 tctcaactcg actccttcat ctaaatttga aacacaaaag cccgaagctc cacctgcaac
    21601 tgctgcctca gctgtttcaa tagctccatg accgtatccg ttcgctttta ctactgcaaa
    21661 caattctttt ccttgcggaa gtcttgcaac ttcatttgct acattttcag ttatagcttt
    21721 tttatgtatc actgctttcg ttgggcggtg ccaagcgaca accatcgttt ttccttcttt
    21781 ctagtcttcc aaaataattt gtgcaaaagc tgtttcattt gtatgagtaa tactgaccca
    21841 tacattgcct tcatgaggtg aacgcgtcac aacaggctga ccattttcat tttttaaaat
    21901 ctcgacatct tgaaaggtaa cttttccgat cccagttccc caagctttag agaaagcctc
    21961 tttgcaagcg taacgcccgc ctaaaaattc tacttggcga tgaaaaggca aggactggaa
    22021 tagtttcatt tcatcagagg tcaagattcg tgcaatgaat tttggttttt cttcgatcag
    22081 ccgagtgatc ctaggaagtt cgactgcatc gatcccaatt ccttttatca tatgcttttc
    22141 tcttttcaat aaatttagaa cactctcatt ctaccaaatt ataaccattt tgccacaaat
    22201 aagtccacaa ataacaaaaa tgaaagtttc tcttaaaaaa gagaaacttt cattttatgt
    22261 ttaatcgtta tttgaacgga ttacaaatcc gcgtttttta tcattgtgac gtttaccgcc
    22321 atctttttgg ctgttattat ttttgctata accgcctttt gatttgtttc cacgataatt
    22381 accgccgtca cgacgattac ggttgtttcc gccaccacgt ttgccatttt tattgtatcc
    22441 ttttttctgc attggcaatg ggcgttctgg tgtgatcttc actggcactg catcagctgg
    22501 atcttttgct gtcgttttta gcaaaagtgc aactaaatct tgtgcagaat attcttctaa
    22561 taatttatct gctgtttgca ggtatttatc tagaccattt tctgcaagtt ttgtttcgat
    22621 ttgttcaact gcagcaccta attgaccttt gaatgcttct ttttcggttg gtggacgtaa
    22681 agtcgtcatc cgttttttcg tcaagttttc aatcacatgc aagtagtcca tttcatttgg
    22741 tgtaacgaaa gtcacagaca taccgccttt tccggcacgg cctgtacgtc cgatacggtg
    22801 aacgtagctt tctggatctt gagggatatc atagttgtac acatgagtga cacctgaaat
    22861 atctaatcca cgtgcagcaa cgtctgtcgc tacaaggata tctaaatgac cgctcttaaa
    22921 tgaacgcaat acgctcattc gtttttgttg agacaaatcg ccatggattc cttctgcacg
    22981 gtaaccgcgt gcttctaatc cgcgtgccaa ttcgtctaca cggcgttttg tacgtccgaa
    23041 gacgattgtt aattcaggtg tttgaacatc aaacaagcga gtcatgatat cgaatttttc
    23101 gtattctttt gcgcggacat agtattgatc gatcaagtca gctgtcattt ctttcgcttt
    23161 gatcttcaca tgatgtggat ttttcatgaa tttaacgcca atattcttga tagctggcgg
    23221 catcgttgct gagaaaagta atgtttgtct ttgatcagga acttgtgaaa tgattttttc
    23281 gatatcttcc aagaagccca tattcaacat ttcatctgct tcatccaaaa ctaatgtttg
    23341 aacggttcca agtttcaatg tgtggcggtt aatgtggtca agcatacgtc ctggtgtacc
    23401 cacaacgata tgtggacgat ctttcaagcc acggatctga cggccaatat ctgctccgcc
    23461 gtaaacggct tgtacacgta cttttttgtc gcgccctaaa cgatataatt cttcttgtgt
    23521 ttggatagct aattcacgtg ttggtgcgat aactaaacct tgtaattcgt gacgatctgg
    23581 atcgattttt tctaacattg gtaatccaaa tgcagctgtt ttccctgtac ctgtctgtgc
    23641 ttgtccaata acgtcttttc cagctaatgc tagtggaatc gtttctgctt ggataggcgt
    23701 tgcttcttca aaaccagcgc gttcgactga tttcaacaat tctggtgata attctagttc
    23761 tttaaatttc aaatgaatcc tcctatatgg ttgttaactg aggatgtagc ttttttgcta
    23821 ccccgtcgat ttgttcggtc aaagtgtatt gtttcttttt gacagtggcc acgcacgttt
    23881 ttatccattc ttagcccctg tggcaccacc gaaaaatcac tttgcgaagt ttaggcatta
    23941 gaaaagccaa acgggtcgcg ttcagcttta tcattattat gacatctggc ttcaacaagt
    24001 tgtttctatg gcaaaaccag tccaatttct cttcatcttt cggcaatagg ttattctaac
    24061 atagtttttc gttttctgca agtttacaga tttttatttc atttgctgaa gttgttctac
    24121 cacttcaaat aagcccatgc tgttgctgcc ttttagcata atactatctg ttgcttcaag
    24181 attttgtttg atagcatcaa tcaatttatt cttttctttt tcaaaataga acacaggtag
    24241 ttgggggtat ttttggttca attcttcttt tagtgcatgc atttgttccc cataaaggaa
    24301 aatcatggcg aatttctcag gatcgatatg ttcactcatt tgcgcatgca tatttaaaga
    24361 ctctttccct aattccagca tatctgctaa cacagcgatt ctgcgtcctt ttgttggcag
    24421 atttttaaaa gtatctaata ccagtcccat ggctgttgga tttgcattgt agacatcgct
    24481 caaaatagct gcaccatttc ctgccgttag ccattcggtt ctgttttgag tgatagaaac
    24541 atgggcgagc ccttggcgta tctcaggtat ggacagtcca aagaagcgcc cgatcgtata
    24601 cgcaatcaaa gcattcgtta cattataact tccaggcagc gggatcgtaa atgtttctcc
    24661 ttctactaca aaatcggttt gtgcttttcc ttttgctgtc acttccgcat gaacatcgcc
    24721 atttccaata ccgaatgttg tgactgtttg tactaatgtt gccgttaaag gtgtcagcaa
    24781 cggttcatct gcgggcacaa cgagtaatcc gtctgataca agtccgtcag tgatttccat
    24841 tttggcttta gcgattcctt ctcgcgaccc aagattttct atgtgtgctt caccgatcat
    24901 tgtgattgct gcagcttttg gttgagccat aagtgaaagt tccgtgattt cattggcgtg
    24961 atccatgccc atttccaaaa cgatcttttc tgtatcttgg ggcatgtgca aaatcgtgta
    25021 aggaagacca atattattgt tgtaatttcc ttgcgtctta tatgttttgt atttttcaga
    25081 caagacagac gcggttagat ctttcgtggt ggttttacca ttgcttcctg taattgcaac
    25141 gacttctgcg cctacttttt ccaagtaata ggttgccaat tgctgcatgg ctttcaaagg
    25201 atctgctaca ggaataactg gaaaattttt tggagcttgt tcaacaggca agctccaaaa
    25261 tgaagcagat gctccgtttg tgatcgcttg ctggataaat tcatgtccat ctcgcgtacc
    25321 agcaagagga acgaataaac ttccttcggt tatcttccgg ctgtcaaatt cgacattggt
    25381 aatctttgtc agtgcagagc attcgctgcc cacggcttgt gcaatttctt gtattgttag
    25441 attcattgag atctctccta aaaaaggttg tgaaaaaagc gtttggccta aacggcgggt
    25501 tcgcatgact ctcacttcca agtaaatttc tctattcgta tttagaagct agaatgaacg
    25561 cggttacgca atcactccag cctcttcttt atttctcttt attcattttt ttcaaaaaaa
    25621 gattgacgct gatggtatcg attcattcct aactggatca gttcttcaat caaatcaccg
    25681 tatttcaagc ccatattttc ccataagagt gggtacatac tgaactccgt aaatcctggc
    25741 atagagttta attcattcag gaataattca tttttatttg tcaaaaagaa atcgcaccgg
    25801 ctcaatccgc ttccacctaa catcgtgtaa gctaacttcg cgtactcttg cgctttttga
    25861 taaacttctt ctggcacttc ggctggaatc tgcatttcga ttttattatt gatatatttt
    25921 gcttcataat catagaatgc tacgtctttt acgacttcac caggcaaagt cgtccgaacg
    25981 tcttcatttc ctaatacagc aacttcgatt tcgcgcgctt caattccttg ttcaacgatt
    26041 gctcgagaat catactgata ggctgttgct aaagcatttt gcagctcttc tcggttttct
    26101 gcttttgtaa tgccgacact agaacccata ttcgccggtt tgacaaacat cggataaagc
    26161 aaagaacctt cacattgatc aaatactttt ttaggatttt ctttccattg attcttaagt
    26221 actggtacat aaggaacttg cggcacacca gcagcttgta aaatatactt ggtcatgatt
    26281 ttatccatgg cacatgcact ggtcaatacg cctgcgccga cataaggcat attcaatgtc
    26341 tctaagaagc cttggatcgt tccatcttcc ccgtttggcc catgtaaaac tggaaaaacg
    26401 atggctcctt cttctttgat ttcgcccgga ttgatcactt ttcctgtaaa gccttcctct
    26461 gtctgtccac ttgggtccca tgaaagatgc aagacatctt tgctggcagg tttttctgtt
    26521 agtaatggac ctttgaccca ttgtccttct tttgtaataa atacgagttg aacttggtaa
    26581 taattataat aaatggcatt taaaactgaa aatgcggaaa gaatggacac ttcatgctct
    26641 gcgctgcgtc cgccatatag taaagtaatc ttcaaaagat tgtcctcctg tattctaaat
    26701 cgttcagtga tttactctca tattttagca caaaacaaaa ataaacgaat aacttattta
    26761 taaaaaaacg agccagcttc taaaagaacg agcgtcattt attttttgtc agctagatta
    26821 tttgtcccgt tttttataaa aagttatgat aaagaaaagg agggatgatg atggcgcaaa
    26881 caaatccacg cagccgcttg gcagaaatca ttcgagtgtt catcaattac aatgtagtgc
    26941 caaactttgt acagcaaaaa aatcccgaac aggtaaaaaa agcctttgaa gaactaggcc
    27001 ccaccttcat caagatagga cagatgcttt ctgttcgaga agatttgctt tcttctgcct
    27061 tcactcaaac attcaaaaca ttacaagata gtgtacctag cgacacattt tcaacggtca
    27121 aaaagacgat cgaaacagaa ctttctcttt cattatcaga tatattcgat gatttttcaa
    27181 aatcgccttt tgcctctgct tctatggggc aggctcatcg tgccaagctg aagaacggtg
    27241 acgcggtagt cgttaagatc caacacccta acatcgccga agagatccga cttgatcttc
    27301 aactatttga acgagcgatc cctttgatca aatacattcc tgaaactagc gtagttgatc
    27361 taaaaggggt actccaagaa gtaaaacgtt ccttgatcaa tgaaatggac ttcttaaaag
    27421 aaagtcaaaa tggcgaacaa ttctatcaaa agaacaatgg ctggaaagag atccgcagcc
    27481 ccaagattta tgacgctttt tgttcgaaaa aagtgatcgt gatggaagaa atgtctggta
    27541 aaaacttgaa tcatttaatg aacgccgaaa acaaaactga aacttttatc actggaattc
    27601 aaaacaagca gctgaaacaa gaagttgcaa agcttttagt tgagaacttt atgaaacagg
    27661 tgtttgatga tggttttttc catgctgatc ctcatccagg gaatctgcta ttccatgtac
    27721 taacaaaaga agaacagaca caagctagcc gaaaaactga aacagtccat gaaaaagagt
    27781 tcggttcctt tgcttttcga gcctccactt ctgcagaaga ccctgttgcg ccatatacca
    27841 tcaactacat cgattttggt atgatggggc atctttctgc aggacttagg caaaaactca
    27901 cacaagccgt tttagcgctg tatacgaaag atgcttatcg aatcgaaaaa gcggtccttc
    27961 gactttgtca gcaagaaggc tcgtttgatg aaagtcggtt ccaccaagaa ctgacaagtt
    28021 ttcttgaaca gtattatgat tctcctattg atgaaatcaa cttgcaagaa gtattcacac
    28081 atgtcgtgac gatttgtcat caaaataatc tgcagttcga tcgtgatatc acgcttttgc
    28141 tgaaagcatt tgggacatta gaaggggtga tccgcgtcct cgatcctgaa gtttccctca
    28201 tggaagtcgc ttcgcctttt gctcaacatt attttctcac tcatctggat gtagaagata
    28261 cattgaaaca aagcggatta gacttattgg aagggatgaa ggctgcgcct aagatccctc
    28321 aacagctcca ccatctatta gaaatgtgga cttcgggaca aggaaaagtc aatttggaat
    28381 tgaaaaagca agacaaatta ttgagcagga tcgaaagtat gatcaatcgg cttgttttcg
    28441 gtatgatttt agctgcattg atcgtagggt cctccctgct tgtccaagca gcacctgtag
    28501 aaaacgcaga ggtcgtttcc ttattaggga tatttacgta tgctattgcc gcattcgtca
    28561 ttattttttt ggcaatcgat gcattgatcc aaatgtataa gaagaggaaa aagtagaaaa
    28621 aggttgtgac agaaagaatc agcttcaaga aataagaagg ggttttacat aaattgtttc
    28681 tcaaattttt gtgaaaattc ggcttatttt tcgaagaggc tacttctgaa acaccgagaa
    28741 taaaaaatgt gaaaaagctg ttctgcaccc agtaataaga acaatcaaaa agggaacatg
    28801 gaacacattt atcctgtttc cctgttccct ttttgatgta ttcgagcata aataaaatac
    28861 cttcgttacc tactatcttc tgtacttggt aaaccttagt caattgcctt ttttaacgct
    28921 tgacgaaaag acatatgatc tttctccgta aagggttttg gaccctttgt ccttatgcca
    28981 gcttttctta actttccact atgataacgt tgttctagaa gcgtgccgat attcgcttcg
    29041 tcaaattctt ttccagagtg atggaacact cgcacaccct tcggcaatac aagtgaagct
    29101 aaaccataat cttgcgtgat caaaaagtca ttcttcttaa ccataccgac gattttataa
    29161 tccgcactat cagctccgcg gtctacgtag acgatttgga catgattagc gtattctttg
    29221 gtcgtataat ggtccacact ggtcacgata accacgtcaa gaccatagga ttctcctaat
    29281 tttatgacat cttcttttac tggagaacca tctccatcaa tgaatagcct cattcttttt
    29341 cgccttcttc cataaatgat aaacagttac taatccgctc agtaaaagac caacattgat
    29401 ccattttgcc aagataagca gttgtacact ttctatcggc actgattcat ggttcagcgt
    29461 gatcaataat agatggattg gctctcccat ttcttttgct aaaacaaaca aaagcgtcat
    29521 caataaaaac tggtggatca cactcccttt attttgagta gtatacttga caacgatccg
    29581 gcgatccatt ggaatagctg tatcagtttg ataatattcc ttatttagcc agctgcctgt
    29641 tactacgtgc cagaaactaa gcagaaagac acctatcgca aaaccatttg gaatcaagag
    29701 gaccaataaa gaagaactca gtccaagcaa ttgcatagcc aatggagacc atgttaaacg
    29761 tttcaaaatc catggtccga ctatttttgc tgcaatcaag cctgctgcat aaggcaaata
    29821 cataaaaatc gaaagttttt ctaatccgta cagtatgcca acataaaaac tgctaaataa
    29881 aaaaaggaaa ttccctagca ttccatttag aaaagtaaac agatttagcc agcttggcaa
    29941 cttccattcc ttcttctggt gtcctaaaac aaacacgaac aagatgaaca gcagcaagaa
    30001 accataaata gcaagatcaa aaagcctttc atcgaataac aatcgggcat ttctcaaaag
    30061 aaacaataag ccaaagaata tcaaaaataa aacaagctct cgcgtagcta ccagatttct
    30121 tttgatgtct ttgaaatcca gttcgtaacg cggataatga ctaaccgtat ggtaggccat
    30181 gacgtaaaaa agtgtataaa tagtaaagct tgctgcaatc tgagtcggtg caggcaactc
    30241 aatcgcttga tacaggagaa atagccagat agctgcaaat aagtactttt tcccatccat
    30301 attgataaaa ccttgcgtct tttcgtgaaa attgaccgtg gtattagcag gtacaagcca
    30361 tgctgcactc agccctagca aaacacttga aaaataaaat aaaggaaaat aaaatacgcc
    30421 taatattcca agcaatgaac cagcaccacc aatcagcaga gcgatcatca gcaacgtgta
    30481 actatctaga ccaaatcgta gactgttgac taaaaatagt ccagtcatcc gaaaggcata
    30541 aaacagcgta aatggcaaaa tcgtcagcca attatgcgag tctgttagtc ccaaaaataa
    30601 aagataagga ataaaaataa ccgtgttact taagataaag ggcgttgttt gtgtaaaatt
    30661 acgcccaaat cgtttgagtt tttcgaacat gacaatggct ccttaaacag tcttataact
    30721 aatctgctct tcgattttag cattaaaggg gaaataaacc aattttacag aatatattct
    30781 tgtaaaaaaa aatacagaaa gaagcataag cttctttctg catttttacg gtacttgatt
    30841 actcaagcag acaagccctt ctccacaaaa gtcttgcccg acacccctgc atccggcagg
    30901 tgataaagat agaaatcaat tatcctgtgc ctccaaaatc aattaggagc tctgtaaacc
    30961 agagtccttc tcgacccgtc gcataaattt actttaacac agttcctatg tttatgcgag
    31021 agcttttttc attttataat cgaccatttg aaatccttga ccgatatctg aaataagttc
    31081 ttctgtaatc tcaaatcctt catgcttata aacctcgatt gcttgatgat tcccctgatt
    31141 cactcgcaaa aagatctctc tttcctttgt ttgcgcagca agttcttcat accagcgaaa
    31201 aactgaagaa gtcaatcctt gaccacgtac tttatttagc aaatataatt tacttaaata
    31261 aatttttcca tttatctctt cataagcagt gtaaccgaca gtcttttctt cgctttttaa
    31321 taaaaagtag gacacacctt ctgtcagttc tttttgaatc tgtttttttg actgataagt
    31381 tttcagcatg taatccactt gtttttgacc aatgatcggc acaaaaactt ccggccatac
    31441 ttcttgtatg actgccatca gctcatccag ttcattttct gttactttta ttttttccat
    31501 acacccacgt ccttttttac attataaatg accttgcgct tgatggaata aaaagagcat
    31561 gatcatgcta tacacaccca ccgaccacgg tttcaagaaa atcacaatct gcataaattc
    31621 cctaaaagat aatttcgtca aaccagctac tagacagatc aagtcgtctg gcgcaaaggg
    31681 aagcagcaag gtaacaacta gtactttttt aatatctttc gtttttcttg tcagcaattc
    31741 atcaaatttc tgatactttt ttggcgataa aataagctgg acaaaggcta cgccatagta
    31801 acggacaagc aaaaatccga ttgcttcccc aatcactagg ccaatacaac tatataaaag
    31861 cccaaatcca ttgccaaaca tcagcatgcc cgctactgag gagatccctc ccggcaaaat
    31921 aggaatgatc acttggatca cttgcaacag aacaaatatc atcactgcat acttgccaaa
    31981 ttgctggata aacgcttgca aagacgtgct cgatcgaaaa atcccatgat gataagcata
    32041 aagaatcaac acaaagaaaa aaaacagacc aataatgggc atgatctgaa gacatttttg
    32101 taaaactgat ccttttttag acatctatat tccccacatc ttttttcatt tattaaaact
    32161 gcctcctt
//
LOCUS       DAZUKC010000027        32048 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000027 DAZUKC010000000
VERSION     DAZUKC010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.028"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..245
                     /locus_tag="ACG80T_001184"
     CDS             6..245
                     /locus_tag="ACG80T_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840849.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            312..521
                     /locus_tag="ACG80T_001185"
     CDS             312..521
                     /locus_tag="ACG80T_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840850.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            581..1252
                     /locus_tag="ACG80T_001186"
     CDS             581..1252
                     /locus_tag="ACG80T_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFS4840851.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1305..3281
                     /locus_tag="ACG80T_001187"
     CDS             1305..3281
                     /locus_tag="ACG80T_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS4840852.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            3294..4049
                     /locus_tag="ACG80T_001188"
     CDS             3294..4049
                     /locus_tag="ACG80T_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS4840853.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            4046..4870
                     /locus_tag="ACG80T_001189"
     CDS             4046..4870
                     /locus_tag="ACG80T_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFS4840854.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            4880..5128
                     /locus_tag="ACG80T_001190"
     CDS             4880..5128
                     /locus_tag="ACG80T_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840855.1"
                     /translation="MKIIRILVFNLSFLAATILCCGLIFSQLSIYPRYQRLFIHFLNI
                     QCASMPLEVIKKLLINPIFMVSLVLLTGLVTWVLGKKE"
     gene            5171..7270
                     /locus_tag="ACG80T_001191"
     CDS             5171..7270
                     /locus_tag="ACG80T_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFS4840856.1"
                     /translation="MENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLANA
                     ANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQDPVED
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
     gene            complement(7308..9365)
                     /locus_tag="ACG80T_001192"
     CDS             complement(7308..9365)
                     /locus_tag="ACG80T_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFS4840857.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(9479..9691)
                     /locus_tag="ACG80T_001193"
     CDS             complement(9479..9691)
                     /locus_tag="ACG80T_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840858.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            9878..10345
                     /locus_tag="ACG80T_001194"
     CDS             9878..10345
                     /locus_tag="ACG80T_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840859.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            10342..10584
                     /locus_tag="ACG80T_001195"
     CDS             10342..10584
                     /locus_tag="ACG80T_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HFS4840860.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            10602..10904
                     /locus_tag="ACG80T_001196"
     CDS             10602..10904
                     /locus_tag="ACG80T_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840861.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            10976..13030
                     /locus_tag="ACG80T_001197"
     CDS             10976..13030
                     /locus_tag="ACG80T_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840862.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            13030..15642
                     /locus_tag="ACG80T_001198"
     CDS             13030..15642
                     /locus_tag="ACG80T_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFS4840863.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            15639..18170
                     /locus_tag="ACG80T_001199"
     CDS             15639..18170
                     /locus_tag="ACG80T_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFS4840864.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PEQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            18222..18554
                     /locus_tag="ACG80T_001200"
     CDS             18222..18554
                     /locus_tag="ACG80T_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840865.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            18555..19148
                     /locus_tag="ACG80T_001201"
     CDS             18555..19148
                     /locus_tag="ACG80T_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840866.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            19311..21005
                     /locus_tag="ACG80T_001202"
     CDS             19311..21005
                     /locus_tag="ACG80T_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFS4840867.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEIVKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            21693..23570
                     /gene="ltrA"
                     /locus_tag="ACG80T_001203"
     CDS             21693..23570
                     /gene="ltrA"
                     /locus_tag="ACG80T_001203"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFS4840868.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            23701..24078
                     /locus_tag="ACG80T_001204"
                     /pseudo
     CDS             23701..24078
                     /locus_tag="ACG80T_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            24078..25283
                     /locus_tag="ACG80T_001205"
     CDS             24078..25283
                     /locus_tag="ACG80T_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS4840869.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMS
                     IINTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            25299..25943
                     /locus_tag="ACG80T_001206"
     CDS             25299..25943
                     /locus_tag="ACG80T_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840870.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            25954..26760
                     /locus_tag="ACG80T_001207"
     CDS             25954..26760
                     /locus_tag="ACG80T_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840871.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            26782..27011
                     /locus_tag="ACG80T_001208"
                     /pseudo
     CDS             26782..27011
                     /locus_tag="ACG80T_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            27557..27832
                     /locus_tag="ACG80T_001209"
     CDS             27557..27832
                     /locus_tag="ACG80T_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840872.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            27874..28296
                     /locus_tag="ACG80T_001210"
     CDS             27874..28296
                     /locus_tag="ACG80T_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840873.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            28315..29907
                     /gene="mobP2"
                     /locus_tag="ACG80T_001211"
     CDS             28315..29907
                     /gene="mobP2"
                     /locus_tag="ACG80T_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFS4840874.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            29919..30254
                     /locus_tag="ACG80T_001212"
     CDS             29919..30254
                     /locus_tag="ACG80T_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840875.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            30401..30595
                     /locus_tag="ACG80T_001213"
     CDS             30401..30595
                     /locus_tag="ACG80T_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840876.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            30627..>32048
                     /locus_tag="ACG80T_001214"
     CDS             30627..>32048
                     /locus_tag="ACG80T_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFS4840877.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSSEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKK"
BASE COUNT        11834 a         4966 c         6548 g         8700 t
ORIGIN      
        1 agtagatggc gcgaccagtt ttagaggcga tggatatgga tactttgaca aaaggaaggt
       61 atgtacagct gttagctcgt ggttattcac ctaaagaagt attcaaagcc ttatctaaag
      121 gaacagatat ggaaaaagaa agattgcgaa aagaattcga ttattggaga acccataatg
      181 gaatcaagga tttgaaacca gcacggccag taattaaacg aaagaaaaag ataacccaag
      241 actgaagtgt agagcgtatg tgatcatagg cttttttatt tttgcaaaaa acaaggaagg
      301 taggaaaact catgaattat gaagaaatcg aaaacagaaa aaaagtgtcg aaagaaatgg
      361 aagaaaagtt actaaaaacg atgaagcaaa aacaccttaa aagattatct gtcgcgcaat
      421 atatcaatga tatgcagtta acaggaaaag agaaagcttg tttgttaggt tcaatgaaaa
      481 actttgaaca gttgagacga acttatagaa ttcattttta aaactttttc tatggaaatg
      541 agaaaaagtt tttttgtttt gtctgaaagg aggcagatgg atgaaaaaat ggttatttgg
      601 ttttttagga gtcactttaa tcgtcttttg cagtgtgttt ggctatgtta gctaccaaaa
      661 acatgaagga gaggtgttta agcaaaatat agaaaaaaag atgcccgtag atcaaatcaa
      721 tgcacatgca aagagttata aggaggatgc aacgaacgtc aataatgata tgtctttggg
      781 gcaaatgttg tccatacaaa aagaagcaat cgaaatgggc gtcaataaac aagtatttgc
      841 acaaattcag attcctgcac ttggtttagc attgccaatt tttaaagggg caaatcaata
      901 cactttgagt cttggagcgg ctacttattt ttatgaagat gccgaaatgg gcaaaggaaa
      961 ctatgtccta gcaggtcata acatggaaat gccaggtgtt ttattttctg atattcaaaa
     1021 gttgtcatta ggggaggtga tggacttagt tagtaacgat ggcgtctatc gttacaaggt
     1081 aacgcgtaaa tttatcgtac ctgggtactt taaattaatc gatggcgttc ctgaagagaa
     1141 ctctttcttg tctttgccaa agaaaggaga gaaaccctta ttaacgttgt ttacttgtgt
     1201 ttatacgtca caaggaaaag aaagatacgt ggtacaggga gagctacaat aacaaggttt
     1261 taatcaacgc gagtcgttga aatatgaaat gaggagagta aaaaatggca ataaatcggt
     1321 atgtaaaaaa agctggaatg cttgttggaa tgttaggaat cattgcaggc tgctttcagg
     1381 ggtatcaagt acatgcagaa gatgtcacgc aaaaaacacc accagagaag gtcaatatca
     1441 cggttcataa attaatgtac gatcaaggaa cgcaattgaa tgttgatatt gacgggatca
     1501 aaaacgatgg ttatacgcac gatcaatatc ctacaggtgt aacgaaatac aacaaggcag
     1561 attatggtga tgttgagttt acgttaggaa atattacgga tcaagtatta ccgagagaag
     1621 attctgatct aaccaacgcg aaagtggacg agattgtaca agatgtagaa gacaagggat
     1681 ctaacagtga gtacgtcaag aatgcaacca acaaaattac ttcagctgta gatgaaaatg
     1741 gtgagatcac ttttactgat caaccagcct atgtcaattc caaagggaat gtatatgtgg
     1801 tttatgaaag taaatccgct gctggattag tcacacaaaa agcaaaacca atggttgtga
     1861 ttgcgccaat gacagataaa acaggatcag gatttttaaa aaatattcat ttgtatccta
     1921 agaacattgt ttctaagttg agttttgaat taacgaaatt tggtgacgat ggcgcagacc
     1981 aaagcaaaca aaccctttta aaaggggcaa aatttgagtt atacaagggc gaacctggaa
     2041 aaggaacaaa attgggtgat cttgtatcgg atgatcaagg aaagctgact gcgactgatt
     2101 tgacgttagg aaaatactat tttgtggaag ttccttcgga agttgtagtg ggatcagatc
     2161 aagaaccaac agcagatcaa tatttattag gcgcagacgc tcgcaacgat gcgcacaata
     2221 aattaacctt tgaaattacg aatgatggcg tgacaagtga cttaaaagct tcatacgtaa
     2281 actataaagc accagtgatc gataaaacag taacaaatgg aacaggacaa gaacattctt
     2341 tccaaattgg tgacgcagta aattatcaag gaacgattca tattccaaca gacattgcag
     2401 gtggcgcaga cggtatcaca gtaaacggtg tgaaaagcga aacttctcct tacagtgtat
     2461 ttaaatgggg agatacagca ggtaaaggac tttcttatgt tgctgaaaaa gcagatatta
     2521 aagtaacaaa taatgatggt tcagtcgtat taaaagaaaa tactgactat aaaatcgaaa
     2581 attctgatag tggttttgtg atcgatttta tcgtaaataa tggacaagta agtgatacag
     2641 ttgcgaattt acatggtcaa gatctaaaaa tgacttataa catgtttgtg aatgatagtg
     2701 ctgaaatcgc aaatccatta acaaatagcg tggatttcgt ttataacaat aatccattta
     2761 atcaggaaga acaccacgaa aaaacgaaag ctgacgttgt gacttatggc gcaaaattct
     2821 taaaagtcga tagcggccta tttggaacag gtatcaaagc aacaccgctt gaaggcgcag
     2881 aatttgcagc taagaatgca gaaggtaagt actacggtgg acttactgat acagataaag
     2941 atggtgtcaa agaagcagtt tgggtagacg atgtagcaaa tgcagcaatc ctaaaatcag
     3001 ataaagaggg tcattttgaa atcactggtt taactgaagg agattactca ctggaagaaa
     3061 caaaagctcc tgaaaactat caaaaattaa caaaagagat ctctttcaaa gtagacaaag
     3121 attcatacaa agaagaaaac cgaatcacga tcaaaaataa tcaaaaagct tccgtaccaa
     3181 tgactggtag caacggtttc caatcttacg tattgatctc ttgcttgttg ctaggagcag
     3241 ggggactttc tgcagttgtt tactttaaga agaaagccta gaaggatcag ctaatgatta
     3301 ttcgtaagcc cccaaaaaga agagtcatgc acaaactgat gatatttttt tggttgctgt
     3361 cggcagtagc agtctattta attccagttg tggaaacaaa tgtagcgaaa tttcaacaac
     3421 agcaaagaat tttagccatt gaaaatcttg aaaaaacaga ttcttctttg gatgttgcga
     3481 aaatagagtt aggcgatcct gttggtgttc ttacaattcc aagtatttct ttaaaactac
     3541 caatttatga cggaacttcc gacaaaattt tagagaatgg tgtagggatt acggaaggaa
     3601 ctggcgatat tacaggagga aacggtaaaa atcctttaat cgcggggcac agtggacttt
     3661 ataaagataa tcttttcgat gatttgcctt cagtaaaaaa agggcaaaaa ttctttatta
     3721 aggtaaatga agaacaacat gcctatcaaa tttatcgaat tgaagaagtt aaagcagaag
     3781 aattacaaaa aaattattta gattatctta tgcctgaaaa agaccaagat aggatcacgt
     3841 taatgacatg cacaccaaaa ggtgtcaact cacaccgctt tttagtgtat ggtgaacgag
     3901 tttccttcag taaagaagat ttaaaactac agaaaaaagc aaaaaatagg aaatcaattt
     3961 cattggattg gcgattagca ctgctattcg cttttttttg cgttcctatt atttttattt
     4021 ataaaaaaat aaggaggaga tcggaatgaa taagagaaaa tggctgtttg tttgtgtagc
     4081 agctctcttt tctactgtag cttattcgca actgtctttc gctgacagca gtgatagtag
     4141 taccactcaa acaatagata attcaattac tgcaccaagc gaaacggaca gttcggagaa
     4201 tactacagaa gaaacggcag attcaaatat aagctttact gaatcaacga atccaacgtc
     4261 agaaagttct tcagagactt cagaaaagcc gcaagacgta gaagatacgc aagtgatcaa
     4321 gcaagccatt catattcaaa aaattatttc ttcgaatcaa atcaacaatc aaggcagtca
     4381 agtagcccag caagaaggta tcaatggcgc gaagtttatt gtctacgata tcacgaatat
     4441 cttagaagaa atgtcacaag atgataatga gaaaaatcaa tctgtagaaa taattgagtc
     4501 aaaattgaaa gatcgagcaa aaaaactttc atacgaccag ttgaaaaaag tggctgaagg
     4561 agaaaccaaa acgattgatg atcaggaagg cgtgattgaa ttttccattg aagtacctgc
     4621 aaacaaaaaa caagcatact atatcgttaa tgaatcaagc cctgaaaaca tttcaaacag
     4681 tgaagatatt gttttattga cacctgtttc aaatgaaaaa ggtgaatttt tgacggatgt
     4741 ttggttatac cctaaatcag agaaatcaga gccgaaagaa gaaattaaaa aagttgtgtc
     4801 aactggtgta aagaagaact tttttgaaaa ttgttgggaa ttttttgctc aattattttt
     4861 tgataattag gatgaaatga tgaaaataat aagaatatta gtttttaatc tatccttttt
     4921 agccgcaaca atattgtgtt gcggtttaat tttttctcaa ttatcaattt atccaagata
     4981 tcaacgattg ttcattcatt ttctaaacat tcaatgtgct tccatgccat tagaggtgat
     5041 taaaaagctc ttgatcaatc caatttttat ggttagtttg gttcttctta caggattggt
     5101 aacgtgggta ttaggaaaaa aagaatgaaa agcttacgat cgtttaaaaa aatattagat
     5161 ggagggcatt atggaaaata aaattcattg cactacggga agagtaatta ctttagtgtt
     5221 gtttttgttc cttcttcttt ttgcgagggg aactgcagtc caggccttag aaaaagttga
     5281 tccgagtcaa atcaaagaaa ataataatct ggtgctttat catgatagtg attcattgag
     5341 aaatatcgaa gtaactggga tcaatggatt taagatacta gatgagtatt caactaaaaa
     5401 acaaaaagta cttgcttggc caaaagtcaa agatttgaaa agtacgtcaa aaatcgttgc
     5461 ccattatcaa aaagtaggta cttatctagg aaaagaaatt gattgtgacg tgactttttc
     5521 taattttgta gcgcacgctg gggttaacca agacggatta gctaaaacaa ttaatggcaa
     5581 aaagtattat aattatttgc tctttgataa aaatatttat cgaggatttt tcatgcagtc
     5641 aatcaatgtc atgaatgcaa aactagaatt taagtatgca ggaacaaaaa caaaagtagc
     5701 tttgagtgaa ccacagccag aaattcaatc aaaaatgaga tcgatgagag cagctggtat
     5761 tgatttaaac gatacatttc tatccattta cagtttaaat ggacagtatc agcaacgtgt
     5821 gtcttggtat aatgacaata atgaatctgt cggtgaacgt aatattgata tgagagaatt
     5881 tgttgggtat gaaaaaatgg gtagcttacc ttattttgtc acaacagata cagcatgtgc
     5941 agaatacaaa gctcctgcgt tatcaacaaa caatttaact tcaaaagtag tgggaggacg
     6001 tgcagaaaag gcttatagct cgaatgatca tttcaccgat gttgtaggag ctgatactta
     6061 tcacagaagt ggtgtaacgt atacgcttca aggcgcttcc ccaacattca tgattggcgc
     6121 aaatacgaat agtatgatgt ttagctttga tactgcattg ctatggacac cacaaccatc
     6181 gaagcctaca aaagaagtgt ttaacaaagc taatactgaa gaggcagcac acaatattga
     6241 caaaaaagtg attccacaag gatcagatgt ttactatcat attcaccaaa agtttgatgc
     6301 attaacagtc aacacaatga acaaatacaa atcatttaaa atcactgata cctttgacag
     6361 caaaaatttt gatatggtat cggatgggaa aaactatgat ggcgcattgt ggtatgttga
     6421 agcgaatggt acttggaaaa aagctgatac aggaggaaag ttaactgtat caggtaatac
     6481 agtcacttat actgcttctg acagcttttt agctaacgct gcaaattacg gtagggatta
     6541 tattttgcag ctccatatga gagcaaaaaa tgatgcaaaa ggaattgcac caaatgtggc
     6601 tactgtaacg tttaacaatg atccgaacaa aggaacacaa aaaacaaata cagtagaaaa
     6661 ctatttctct cttaaaccaa ataaaaaagt ggaacaagat gggaaagatg ttgatggtcg
     6721 aaatgatggt aaaaaaggaa cgcctacagc tcctttaaat gcaggatctg aagtacaata
     6781 tctagtcaca caaaaatggc atacaaaagg tgtagacatt gcctcagatc attacaagca
     6841 atttagtatt caagatccag tggaagatcg tttgacgtat aaagaaggtt ctgcgcgagt
     6901 gatagatatg agtacaggaa aagatattac ttcagaaggt acactaacct atgatagcaa
     6961 ttcaagaacg ctgaagtggg aagcttccgc tgatttctta agtaaaaatc ttttagatgg
     7021 acgagaaatt cagctgatat ttacagctaa aactcctttg aatgaagaat tggatatcga
     7081 caaccaagca aaagtttcag tagaaagtat tgagaaagca actaatgtag taacgattgg
     7141 ggtaaaacct aacttacctc agatcattgt gccaaaaact ggatcgaatc atttagtgct
     7201 aactgttatt ggaagtatat tatttcttac tttagcatca ttgggctatg tagttttaaa
     7261 actcaaataa atataaaaac aaggctgaga atcagccttg ttttctttta tttgaagatt
     7321 ggattcgcac taataaatac attatctacg ccaattctac cagcagagtc aactacaaaa
     7381 ttatgaccta atccgagaat actttggtta ttataaagta aatttgcatt atgggcaggg
     7441 ctatttttcc atcctaccat ggcatcgata gcaccatcgt ctgataaaag gtcatcaata
     7501 tacagcccag tttgacttat gttttctccc ttagcaccgt agcagcctaa gctttccaat
     7561 aaatcttgga aagtaccacc accagggcga tagtgactga agctgacttg taattcttta
     7621 gctcgaatat ctgcagcctg ttgaacaata tcattgaatt gcattggggc aacaccattt
     7681 tgttggcgat aatcatttaa ccaagcaagt tctttttgac gcataaagtt acgtaattcg
     7741 tctaatgaaa tctttttata tttgaatgtg attgttttat tttgatcgac gttattaatg
     7801 gatcgttggc cgtttttatc cacggtaacg gtaggatcgt catttctgca caagaaataa
     7861 ttttgatcag tcatgttttt ccaaggagta gcaaagctat ccccttcgta tactgttttt
     7921 gattcactag agagaacagc gttggtatca gcatttacat gattaacagt cacattaaat
     7981 ttacgagtgt agttgaaaat ataattttga ctttcagtca cattgtttaa ggttactgaa
     8041 tttgttggtt ctacccgatc gtctaaaaca tatccttgaa tagaaggagc agttaaagtg
     8101 acactgtcac cgtgagtgac attgactatc tgttctttca aaatattaac tcgtccttcg
     8161 tttttgtagt tgttattgca caacaaattg atttgatgca cttgattata attgaatgtg
     8221 attgttgtat tacctgtgat tgttttgctt tgagtatcat taccaatcac tttgtagcta
     8281 aaattatctt tagcgccttc gctatattta aatgcttttg ccttagcagt gtaagtagaa
     8341 ccagaagtta cttttgcttg atctgtagca atcacttgac ctgtttgatt gtcaatatgg
     8401 ttaatagtaa ccgtatattc ttttggtgtt tcaggtttgt gttgcaaccc tttaaatgca
     8461 gcatcaagac tatttttagc attgtttact tgtgtttgag aaaccttaga atcgtctagt
     8521 acttttttag cattggataa agcagtctga aaattattcc aagattcttc tgtaaaatct
     8581 cctttagcag ttgattttac tcggttgtat aaagcttgta attcagcttt attcacagta
     8641 ggaactggtt ttttctttaa tcctttgtaa gcagaatcta atttattttt ggcactatcc
     8701 acttgctttt gagtcgcttt tgagtcattt aacactttct tagcattgtt taaagcggtt
     8761 tggaatgcgt tccaggtatt gtcggtatag tcaccttttt tcgttccctt aacgctcgta
     8821 taaagagctt ctagagccga tttattcact ttgggtttag aggtgtcagc gccataccaa
     8881 ccgatacctt cgtatttcca gccatagtct ttggttaacc attgtacttc tttccaactt
     8941 gcagtgtagt gatgtgagcc agattttaca ccaggaagga aaaggcggta gatcggttta
     9001 ttattttttt cggcagaacg ccaggcaggc ccttcataag tccagccgta atttttagtc
     9061 aaccatttca cttcatccca actagctgtg taatggtgat cacgaagacc tttattgtac
     9121 aaacgataga caagatggcc accgctagtc ggtgcttccc aggcggggcc ttcataattt
     9181 ccccatttta tctttactag atggtcacgt tcttttttgc tagaggtgta aaaatgttca
     9241 ttattatttg gattgtaaag gcgatacatg acgttagttg aagcagcgtc aacagatcca
     9301 gcaagactaa gtgaggcagc aaatgttcca atagctaaaa cagtttttgg attaaataat
     9361 ttcatttttt caactcctaa atttaattaa aaatgtttac atgattatta tatatcggct
     9421 aaccgatata gtcaataaaa aataagaaaa gccttatata attttggctt ttcttatttt
     9481 atttgttagt tgaagtatta atatcaacga tttcttcagc caatttattt aatttcatag
     9541 catttatgaa tctcatgttg tctatactag agcgtttgtt ggcaaggtca cttactgtag
     9601 ttaagggtac gccagattct atggatatag aacgaactga tttaccacta tcaaacagca
     9661 accattcgat tttcttttta tcagcgtaca tttcaatgct ccttcttgtt ttttctttat
     9721 tatagctctt ttttgtaatg aatcacaata tcaagagaaa aattttctat agaaaggtgg
     9781 atttttatga gtattcgttc tccgtgtatc caaatgaggt ttgttgctac tttcgttatt
     9841 tgatatacta aaaataaaca acgaaagaag gaataaaatg aagaaaaagc aactatggtt
     9901 tgtctctatg tttgcaatat tttttattat tttaagtgga tgttcaagta ataagaaatc
     9961 ggaagctaaa gaagataata gtttattgca accaggaact gtttggaaag aagaagtagg
    10021 tggtcttatt cataatttaa aaattataga tgatactacc tgggaatatt ctgaaagtgt
    10081 atggcatcca gatcctgtac aaataacagt taagaggcaa aaagactatg aaggtttaga
    10141 aagatacaaa atagtagatt ctgcaggcat tagggaattt ataaacaaac ctgattcatt
    10201 atttattgtt gttccctatg agaaggatgg cgtaaaaaaa ataatttttc ttggatctag
    10261 caaggatgag aagcgaacga aagagaaatt aattcatgat ggatcagagt taaataaata
    10321 caaattacaa aagactagtg agtgattgct agtcttttta tttatcctga aggaggaatc
    10381 acagtggcaa aagttaaaac accattcgaa aaagccgcgt ctaaaatctt agaacgccgt
    10441 ggtgtttctg aaatacagtg gaaaaaagaa gttcttgaaa aagcacaatt tgacctatta
    10501 cgaggaaaag ataaggactt agaagaatat atctatcaac gtgaacgtga aaaacttgta
    10561 ttcgatgaaa ttattaaaca ttaaaaggag cgaataatct tatgtttgaa tcaattacaa
    10621 tgaatttttt aactcaaccg aaagtagtag ctgcagttga tatttttaga gcattacaaa
    10681 ctgccgcaac tagtttgaaa aataatctga tgatttttgc tgctgttgcg gcagtggctt
    10741 ccattgctct tggaggtatt ttcttcttct ttgggcgaag aggagctgaa aacgggaaga
    10801 cacacgtagg ttatgtattg ttgggggtag gagttatttt agctgccgct tctattgtag
    10861 tttcaatgtt ctcaatgttt ggacaaaccc cacaaggact ataaaaaaaa caagtaggag
    10921 gagtaaaaag tacgtggcta ctttttactc ctttttttat gaaaggagaa tattcatgtt
    10981 ttttacgatt tcaggaaatg caaaattgga taaagagtta ataagaaaag caacgaagaa
    11041 agtgggaaag cgtacttcct ttttaccgaa aacaattaaa tattctgtca ttgaaacgga
    11101 agaacagcta catgtgttag gacaagtgtt agaacaagag aatcaatctt ctgttaaatt
    11161 gactctcacg ttacatgata aagaaggaaa aatagaaaag gctggcacag aatacgtttt
    11221 tggcccactg gattatcaaa tttcagaagg tttttatcct acattgatag atttaatccg
    11281 ccttgatgtg tttaaagata cggatgaaac tatgagtgtg gaagagtctt atcgaaacga
    11341 aaaactttta caagttttag aagaagagtt agagagcgac caaagaaaag tgaatcaagt
    11401 gagtgaagtc gaagtaaatg aagaagtacc atgggaacga ccaatctttc caaagaagga
    11461 gtcaaaagaa aaagagacag tctatgttga cgaacaagct gatattccga ctcagaaatc
    11521 gatatttgag gaagagaacg atgtgatggg tcaattggag gtagatccct ttgaagaaac
    11581 aatggaggat gaagagacgg acgaagtaga agttgatcca gttgaagcta atgaagagtc
    11641 aacatggact gaaggggatt ttcaggaata tattgaacca cttatcagtg aagaagtaga
    11701 aaaaaacgaa acagagatac aaaaggcaac tgtatttgat tatattttgg atcaatatcc
    11761 gccagagcat gaatggatga aagaaaaaaa caaattattt ttaaatgagc tttattcgaa
    11821 atatcaatta ccccaaacct atcaagcatt tttagagagt aaagaagaat taatctcaca
    11881 gggtaaaggc acacttttag aacagttaga gtctgtccaa tcaacaaatt ggcatgagct
    11941 tgcacaagaa gatttattag ccacctttga agaacgtcaa caagaaacag atcaagaaat
    12001 taatgagtat gcgacggaac agtcgtctaa ttgggagaaa gtaaaagagg aactaaataa
    12061 agaagaacag cgagtgattg aagaagaagt acaaaaagtc aaagagcggt acgaaaagaa
    12121 acgcgacaat gcttattcct tgtgtgtaga aaagatcaac aattttacta agacgaatca
    12181 agaagcgtta tcgaaagaaa aagaaaactt gttaaaagaa cgtattaaag aacgaaaaga
    12241 agagttctac cgcggtttac gtacagataa gctaaagatt agcaaagaat taaatgaaca
    12301 actgaataag ctatatcaaa caacgactga agttcttatg gaaaaacacg aacagatcca
    12361 gcaagaatta caagtacaaa ttccaaaatg gaaaaaagag tatgaaattc aacaacaaga
    12421 attgaaagaa caagaagaaa aacaacggca aaaagaaaaa gagcgtgaac aattagaaat
    12481 aagaaaacgg gaacaggatc ttgcggaaca aaaaatggcg atagaaaaac aacggttaga
    12541 gcaagaaaag gaaaaagaag cagctgctcg taaggaacgt caagaacaaa tggatacatt
    12601 gcgtcaagca cagttgaatt atctagcaca acctcaacca gtagctgctg ttcaaacgcc
    12661 agtgaacaac caagcaaatt taactccttc accccagcgt tcctttaacg gatggatggt
    12721 gggatgtatt gcaagtttaa gcttgcttct aggtggagga acagtatttg cttttaatca
    12781 tatcaatcaa tcttcacagg ctgattctgt agccacaacg gcgtcaatca aggaagaagc
    12841 ccaggaacaa gcaagaaaag agtatgaaca acaattagaa gaattattga atcgtaccaa
    12901 tcagtcaaca acagagtcaa gcatggaaaa tagcaaagag agtgattcta gtaaggctaa
    12961 agaaggatca agcgattcaa ctgtacaatc aaacacttcc gaaagttcag aatcaaatga
    13021 ggtgaaataa tgcgtgttag agaaacaacg aagcgaaaag acaaacgatt caatttagaa
    13081 aagaataggt atctttctca tttctatcgc tatactccct caggagaaga gctaccagaa
    13141 aaacatgaca aacgcatttc gaaaaagatt ttttatagtg gacttgtata cctcccttta
    13201 gagtatttag taacaagagt accagaagtt ttcaaccgat tggaaattgt taagggaagc
    13261 ttacaaggaa taaatctgtt tcaattacag gaaatgccac aggtctttca ttttgattca
    13321 gctggttttt ggaatccatt tactcctaca actagtttgg gtacacgttt agcaatttcg
    13381 acagtttgtg tggttccagc tgtacttatg ggattgaaaa tggacatgtg gtttcgtcct
    13441 ctagcagatg gacaaaaagg ggataatcga ttaatgacct taaaagaaat ccaacagatt
    13501 tatccgcaaa tacctgaaac aaaaactact tttccaggtt atggaggtat tccaatcaca
    13561 cattacaaaa aatattggta cattcaaaca gatactgtta atacgtgtat tgttggtacc
    13621 tctcgatcag ggaaaggtca gattgaagtg ttagccacca ttgacaattt atctcgtgca
    13681 gaaaaacaaa gttcaatggt cgttaatgat ccaaaaacag aattatatgt tggtagtaaa
    13741 gatgaactag aagctagagg gtatgatgta tatgccttta atatcaccga tccactgcaa
    13801 tcaatgagtg ataatccatt ggcactcatc gtgaaatact ggaaacgagg agacatagat
    13861 actgcaacac agttaaccaa tactttcaca aatacgattt attatgatgc caacgcttca
    13921 gataacaaat actttaacga aaacgcgcag aaagctgtca atgcgcttat ttttgcgttg
    13981 ttagaacaag ctgatcaaac aggagattac tccaaattga caccgaataa tctcgttgag
    14041 cttctttctg agattggcgg ttttaattat tatccagatc cagacaatga attcaaacag
    14101 ttgaatgcct tagatgaatt tatgaatcag ttgccacctg gaaacattgc aaaaaaacaa
    14161 tatggggcaa cgaaaattgg atctgataaa gcgaagggaa atattttatc aacggcaatc
    14221 acgggattga atccatttac tctaacgaaa attgcaaaga tgacttctca atcaacgatt
    14281 gattataaat cggttgggtt tccaaaatac ttagatttga aattacatga aagtctttta
    14341 aatcaacgag tcgtagtaga attttatcat aagggaaaga aatttcatga agaacgtgtc
    14401 aaagtaggct ataaaggatt tagtgaaatc aattttgatt gtcatttagt tgcaggtgat
    14461 caagtttgtt taaaatacga tcatcaagga aaagaatatg tggcttggta tgaattttct
    14521 caaagagtat tgaaagacga agagggtaat atcgtttatc ggaaaaaacg aggagaaacc
    14581 catttgccag aattagaaaa agaagccgtt ctaacattag atgaggaaaa aagtaactta
    14641 ttagtagagt ctattcggat gcactatacc gataaaccaa tggctatttt tatgcttaca
    14701 cctgattatg atccatctaa ccatatcatt gttagtattt ttcttagtca gttatataaa
    14761 gaattatcta cgcaatgtgt taagactaaa ggagataaat gtcaccgcag gattcatttt
    14821 attttagatg aatttgggaa tatgccacca atggataata tggaaggaat catgacggtc
    14881 acagctggcc gaaacatgtt gtgggatctg tttattcaat cttatcagca actttatgcg
    14941 aagtatggag aacaaaacgc agcgattatc aaagaaaatt gtcagacaca tatttatatt
    15001 atgtcgacag atgataatac catcgaagaa ttttctaaaa aagtggggaa taagacggtt
    15061 gaacaagaga acagtaccat caattcatta ggtatgaata ctcatatcaa tcgcaatgtt
    15121 gattcagatc gtatcttaac tcctgaaaga atttctactt ttttagaagg agaaacgatt
    15181 gtactcgctc ctttaaaacg acaagatttg aaaggtcata aaatacggcc gtatcctatt
    15241 tttaatcatg gggaaacgaa tcttccttat gcttatcaat atattgaaga tttcaatcca
    15301 tcgtgggata tcaatgatat agacatacat tgtacacatg cggacttgga tcttaaggct
    15361 ttacaaattg attatgatta tttcctacca gatgaatatg ctagaaatct ctatcatgga
    15421 ctgcatgatc aattagaaga aaaaaacgaa gaaaatatgt tttataaaat cgttcaatct
    15481 cttgagaatg agacggctaa aaaattgtta attcaatttt acgaagaaca agcagcagat
    15541 cgacttcaag aatatgttga aaagttgatc aaaaataaag aaatttctga aatgattggg
    15601 aaacggttgt tattggaatc tgaaaaggtg gtgaaagaat gatcattgga ggaatttttt
    15661 ctggcatagc cgactggtgg tcaggcttat tcggagaagg ggatgcaaac cccaaaaccg
    15721 taacgaaagt cttacaagat tatgggaatt atctgcagta tcaagatgta tttagttgga
    15781 tttggaacac ttttctgtgg ggattgatta aaggattata taatctcaat actttactag
    15841 aaaaaaccat ttatcaaagc tttgatttaa aagacttatt gaacgccgct ggggtaaatg
    15901 cactatttca aagtttgatt agtaaggtat tggctctttt ttgtgtcgtc acattaattt
    15961 atttaggctt gaaatttttt atgagtaaac acccaccaaa agtcaaaaat atcttggtta
    16021 atttatttat ggccatgatc ttgatttttg gtggtagttc tttaatcgat gaagggttaa
    16081 atatcagtaa aagcttttac ggagacgtca ctgcagcaaa taaagcagat aaaacgggat
    16141 cacctgcttt tcaaattatt caaaataatg tgatcgatgt cagtacgtta ctagcaactg
    16201 acccgaataa agtagaatcc ctaccaacag ataaaagaaa tgctttaaca gcgaaaaatt
    16261 ttaaaacagc gaatatcaat gggattatta ctcctgaaca agctaaaggt atggcaggta
    16321 atgataaaag tgggttatca gatatacaaa aggatcggtt aaaggcatta caataccgat
    16381 tggaactaga tgataatggg aaagaaattc ctgttcaaat tagtgacgaa ggcttagcaa
    16441 aatatgtcta tacttcaggg tatcgtcgat acagtagtaa accaggtatt attttagctg
    16501 gtgaatcttc tttggcagta gcctatttat ttatcctatt tacgatcgtt acttgcatta
    16561 ttgagctggt atttaaaaaa ttctatttag tgattgccgc gtcaacggac tttgagacag
    16621 gtcaacggat gaaaagtgcg atagaagata tttctcaatc cttcttgtta ttggcgttta
    16681 cgattttaga attacgaatc tataccttaa tgctttcagg tatcggtgat ttgcatgcgg
    16741 caggaaagat caatggattc ttgtatgtcg ttgctttgat tgtactaacc attgcgctat
    16801 tcaaaggctc tcaatcagtt acgaaaattt tcggcgttga tacaagcctg aaaaacggca
    16861 gtaattctct tcttagcatg tttgccttag gaaatattgc gaaaaatgtg ggtagcggag
    16921 ctaaaaacct tgcaagtgct ggtaagagtg gtttaagcca tttaaatgat attcgtaaaa
    16981 atggatttgg ccatcgaaaa gatgttaccc ctgataacgg ggaagaacaa tcagacgata
    17041 aagctacaac tgtacctaat agtaagaaaa cagtttccga aatgttcggg aaagcgaaaa
    17101 atggtgtttc taaagccgca gaaggtcttg gttacgtcaa tgaacgtggg ttaaaaggaa
    17161 cagctgaaga tcttacagaa aaagccaaag acaccttaga agataaatta gacggcacga
    17221 gagctaaaga attggctgaa gctgtgaaga atccatccga agcactagaa aaacaaaaag
    17281 aaaaagcttc agaagtcaaa gaaggactaa aagatgcgta tggtgaagga caagtcaatg
    17341 cagcagtgaa agctaataca acaaccgaaa aagaaaatca agaaggtata gtgattcctt
    17401 cagtcaaaga tcaccaagaa gcgttgcact ctaatgaaaa ttcgattgaa gagaaaaatt
    17461 ccatagaaga aagaaattca gaagaaggaa aaaatggaca gttaccaaaa actgatccag
    17521 aacaatatcg ctataaacct aacagtgacc ttgaaaatga gaagcgaatt tcagatttat
    17581 caaaagtaaa tcaagaagac aaagcagaaa atgagggaca gttaccaaaa actgatccag
    17641 aacaatatcg gtatagatct gttaactccg aaaatgaaaa acgcatttca gatttaccaa
    17701 aacgaaatca agaagtacaa acggatgagg attccatcgt tctgccaaat gataatggaa
    17761 tcgaagttgg aactgaaaaa tcaacgaaaa ttggtttacc taaggcacaa aatttaccaa
    17821 tgaaagatag tcgtttgaat caattcaagg ataatgatca aagttttgtt catcaatcac
    17881 ctgcaggtac tgttcaaatg aaaccaagtc gtttgcctga aaatggaaat ctgacaactg
    17941 tttcttacaa tttatctgaa agtggtgaga cagggaatgc gtccgttcaa aatgttgtga
    18001 caagagaagc tgcaccaaaa cctattcaaa acgttcatac aggttacaag ccagcaaatg
    18061 tggcatttac tgcaccaaca aaaataaatg tgacaagaga aacaaaaaac aggaacaatg
    18121 cattgaaaga acaactattg agggatatca aacaggatat caaaccataa aaggcctaca
    18181 agttaggcct tttttctatg tatgaaaagg agaaatcatt tatggataaa tatagtaaca
    18241 tgaaaaaccc tccaagattt agtggagaat ggaagttttt cgcttttttc tttttagatc
    18301 tattaacgct tggtggctgc tttttttggg cgaaaatggt caataatctt gtccacttac
    18361 cactcttttt tgcactcttg aattatggct tatggattgc gatagcgtta gtgattgttt
    18421 ggcgacccta taataaccca ggtcaacgac aaatttttgc gattatcgag tctatttttt
    18481 ttgcggacaa gaaccattat catgcgttag atcctaaccg aaattatcat ggtacgtata
    18541 aggaggatga ataaatgcga gtcaaaggac aaaattcagt tgaaaggaaa tccattttgc
    18601 agtttggaaa accagaagag cgctctatct tggacttatt tccatataaa tcctgtacag
    18661 aagagggagt cttaattact agagaagatc attttcaacg ttattatcgt gtagtttcta
    18721 cagacgtgga aggattaaat gaacaagaga aactagaacg aatgaatcag ttgaccaccg
    18781 tcatgcgtac gtatgtgcca acaattaagt tagttagttt gaccacagag acggatctaa
    18841 cggaacaaat cgtgcaaaag cggcaattat tagataaaaa tcgtatggaa cagtccttag
    18901 gaagaaatct acaacgatta aaaaaatatg aaaagaaatt agtcgaagaa atccaggaat
    18961 tgaaacgagc agaacaagaa cgtcctgact taagcttctt tttcgtcatt gaaggaaaga
    19021 caatcaagga tttacaaacc agaaaccgtc agttaatgcg atctagtggc attttagggt
    19081 taaaaccact ctacaaaaaa gaattgatca agattctgta tcgaatgaac aatatgaatg
    19141 atgagtagga atggagaaga gagaatgaaa tatccgttga tatggttaga aaaagatgtt
    19201 ccgaaggaag agaccactag aaaatttcct cacactttaa gcctgggtaa gttgggaaaa
    19261 gggcaatctt ttaaccaagt aaagaatcaa acaagaagag gtgactgtac atgttagaag
    19321 caattaaaac agctttttca ccacaaaatg atcaaaaagt acttgatcca ttaaagcaac
    19381 taggaaatcg ggaaatagaa attgtaaaat ccaaagggta tgatttagat tttttaaaac
    19441 aagtgatgcc acaaggtggg ttaaattttg atgaagaata tgtgacgtat ggtaatggtt
    19501 attcacgatg tgtgacagtt tatcactaca caaaaaatcc tacgcttttt tggttggcca
    19561 atctcatgaa caatcctcat acagtggcca cactcgatgt tagcacagat gaaaagcaaa
    19621 aagtgattga tcaaatcaat cgtgccttag atgaattaaa tgatcgatca gataatgaac
    19681 gaaaagcgac tgaccgaaat aaaagcgcgg aagaatacct ggaactagcg gaatatgcta
    19741 gaaaattgac ttctaatggg gaaatttcta agcaagtcaa agtgagagta catgtttacg
    19801 gaaacacgat tcaagaagta gatgaacgag cgcaggaact gatcgaaaat ctcaaaggga
    19861 tggattatcg tgcggtgact tatctgcata aaaccaaacg agattggcaa tcgatgttta
    19921 ccatgttcga tgaacaagct gaatggttcg gaaatatcaa agggcaatca ctaccatcgt
    19981 taaacattgg cggaggtttc ccgttccacc accaaacgct aaaagatcca ggagggacac
    20041 aatatgggga aaccatgaca ggcggcccat ttgtctggaa tgcgacgtat aaaaatagtg
    20101 tacggttgtc tactaatacc ttgcttctag gaatgatggg agctggtaaa tcgaccgttc
    20161 ttaaaatgat cgcggaagcc catttagctg caggagactt tatttggggc tttgaaaagg
    20221 gaaaagactt tattcctttc ttaaaggaat ataacggtat tatggtgcgt ttagacggat
    20281 ctgacggaat gatcaatcct ttagagatat ttgccacacg gacgtatgat gaagcatcca
    20341 gtttatatga cgatggatca gtcaaagatt tgaagatcaa tgaagcagcg tcctatcaaa
    20401 cacacttaga caaagtggtc tatcaggtac agttagtgag tcctcagcta aaaggaacga
    20461 tgaaagcaga atttaagacc tacttaaatc gtttttatga agagtatgga acagtaccac
    20521 gcggctttac ttcctcaaat tcgcgttcaa atactgatac gcaagtcaca gggaaagatc
    20581 cagaagctta tccgacgttc aaagaatttt tagacttttt aggacaactg gaattaccag
    20641 gtgcttccca agagaaaaag aatcgaaaag aagaaatgga atccatcgta gaatccttat
    20701 gtgagacgta tggcatgatt tttgatggtc atagtacgat tcgacacctt gaccaacaac
    20761 agttggtctt ttttgacatt gactctatta caggcttaga tcgaggggta caacaatgtc
    20821 aaatgtacat ggctatgacg ttgatttgga atcaggcatt gattcaagga agaagacaac
    20881 gacgtttaat taaagaaaaa caaatggaag aacaagactt caaacgcttc ttggcgatga
    20941 ttgatgagtg ccataatggt gcgacctgtt ctgcagtgaa aagctgtggg ttgcaagtag
    21001 tctaaaaact attgtaataa ctcttataac gagaagtcaa atgagtgggt aacgccacaa
    21061 agggcttcca ctgatactcc gacatgcaac aagtagttta tgcagaccaa cgttgtgtga
    21121 agctcggtga agtcggctga aggataccgt aaggtaaaag aaatagtgaa taccacgaaa
    21181 gttctctaga ggtaaagggt gtatccgata agtcgggtgt ccaaaaaaac ttaatatggt
    21241 gagtatgtac aaacgtactg acgaactagc gaatgtactg tccgaaaggc atagctccta
    21301 gaaatgggag agacagtgag caaaaaatca ctcattgtgc taacttgtgg ttgagtaaaa
    21361 gtcgttgtta tgaaagacca tgctgcgtta caggcgtagg agaagttagg gggcaaaagc
    21421 tagcatctaa gttaaggcat gtggatagat ataagggaac aggggaagcc atcaacatgg
    21481 agagtgcata ctcgatgaag tggtagtaag aaatatgtga atgacaaata acttacttta
    21541 gtgatggtga aagtagagtc acgactgatg aaggttctgt aatggaactg gaggaacagg
    21601 ctcgattcgt ctgtaaaagt catgaaaccc gttcaaccat gggattctcg tatgactaaa
    21661 aaggtcacga gccaattgga gcgtgataaa ccatgacaaa agaaacaagt ccaaaagata
    21721 ggaagttgcg gcatgcagaa tattatggca tgacggaaat cttcgataag atatactcca
    21781 aaagtttgaa gggagataat ttcaagaatc taatgcagat tatcatttct gataacaaca
    21841 ttgtcctagc atatcgtaat atcaagagaa atcacggtag tgtgacaaga ggagaagatg
    21901 gagtaacgat taaagatatt gaaaatctca accgtaatga attcgtagaa attgttagga
    21961 agcgattcgc acattataac cctcggaaag taaggcgtgt agaaattcct aaatataatg
    22021 gtaaattaag accgttgggt attccatcaa tatgggatag aatcgcgcag caatgcatct
    22081 tacaagtatt agaaccaata tgtgaagcaa aattcaataa tcacagctat ggtttccgcc
    22141 caaatagatc agcagaacac gcaatagcag attgtatggc ccgaatgaat caaagtcaca
    22201 tggaatatgt ggtagatgta gatatcaaag gtttcttcga cgaagtcaat catactaaac
    22261 tcatgagaca aatctggtct atgggtatac gagacaaaca gcttctctta atcattagga
    22321 aaatgttaag agcacccgta gtacttccaa atgggaagtt gatataccca acaaaaggaa
    22381 caccacaagg cggaatactt agcccattac ttgcgaacat caatctcaac gaatttgact
    22441 ggtggatagc aaatcagtgg gaggaaaaag atgccaaaga gctaaaacat cattttagga
    22501 aaaatggagc ttgggataaa ggtaatatct atcacaagct cagaaagagt acaactctaa
    22561 aagaaatata cctagtgaga tacgcagatg attttaaaat tttctgtaga aattataatg
    22621 aagcgaaaaa agttttctat gcaactaaaa tgtggttaca ggagcgtcta aagttaccaa
    22681 tatctgaaga aaagtcaaaa atcacaaatc tttcaaaaga agaaagtgaa tttttaggct
    22741 ttacgatgaa attagtgacc aaaggtaaca aaaaggttgt taatacgcat ataggtacaa
    22801 aagcacaaag catgattaaa gtgaaactaa aaaaacaaat caaacgaatt caaaaaagtc
    22861 ctaatagcaa agataccttt atctatattg gagtctataa cagtatggtt attgggattc
    22921 ataattactt caaaattgcg actcattgta gtgtagcaat gcgaaaaatt gcaagagaac
    22981 tcagaccaag ttttgaaata cgactacgag aacaaggaat ctcaaaagaa ggacaatacg
    23041 gaaagtacga aggatacaag ccttacctac ggtcaggaca aatacgatat ataagagggt
    23101 ttccaatatt accagtaggg tatattcaac acagacatcc gatgtgtaaa aaaagaaata
    23161 ttaataaata caccgaagaa ggtagggaag ccattcataa gaagcaacaa accgtagaag
    23221 aatggaagat atcgtggttg tgtagcaatc cagtgcttag taagagagca acgattgaat
    23281 ttgcggacaa tcgaatttca aaattcatcg ctcaaaaggg aagatgtgca gtaacaggtg
    23341 aggagcttat cttatctgaa atgcattgtc atcatattat cccttatcat gaaagcaaaa
    23401 gtgatagcta tgaaaaccta gtgattgtta ctgaggaggt tcatcgggta atacacgcta
    23461 cacaaagtga aacgattgag gaacttctaa aatatctaaa attaaatcca aaacaaaaag
    23521 aaaaactcaa tgagttgaga ttaaaagttg gtaacgaaga aatatcttaa taaatatttg
    23581 taggacggga aaacagaatg tcttttatag acgagggaac gccgtatgcg gtgaaagtcg
    23641 cacgtacggt gtggactggg ggaaaagacg gagataatat caaactctta cctatcagta
    23701 tcgattaatc cagattttat tgaaacggta aattatgtca caaactttca acgagaaatg
    23761 cgtaaagtag aagcaatgac tgttttagct acacaatccc cacaagaaat ggtgccagaa
    23821 aatatgtctt ctgataactt ctcgaaactg aagaaagtct ttgaattttc ttctacgaag
    23881 atctttatgc gtatggatga atctatttta cgtcatatag aaggattagt gggtaaatca
    23941 atgacgaact cagaattaga atccattcct caacaaaatc aaggagacgc tgtgatcaat
    24001 tttggctcaa aagaaaccat tcgtgttcat tttagtccga acaaacgtca attaaaattg
    24061 tttgatggag gaaaataatg gaaaaccaaa atgaaagttt aatcaagcag tatgttaagc
    24121 gtcgtgcaaa gcggcgctta tttttgtggt tgtttggtac ttctgcaggt ttgattacta
    24181 ttttgatcac tgtgttcgtg accctatttc taatacttgc ggcgggctcg attgataatt
    24241 cggattcaga ttcaagcagt ggtggcgaag cttttacagg agaatattcg gaaggcttac
    24301 cgatttataa agaaatcaaa ggaagaggcc ctttttctga tgagattgcg caatatgccg
    24361 taggtgcagc agtgaaatat aaattattac caagtgtgat cctttcgcaa tacggttacg
    24421 aatcagcgtt tggtacatcg gcgtccgcaa gaaatgatct caactatttt gggattactt
    24481 ggtttgatgg ttgtctgttt cctaaaggaa ctgcacgagg aattggtggc attgaaggtg
    24541 gctggtacat gaagtttccc aactcaaaag ctgcattttc ctattacggc tttatggtag
    24601 ccacacaatc caattttaat gcttgtgtag gaaataaaag ccctggcgca agcttattga
    24661 tcttaggacg aggcggttat gccgctgcag gaattactga agacagtgcc tattataaaa
    24721 attgtatgtc tattattaat acaaataaat tgatggagta cgatgagttt gcgattaaac
    24781 actggagaga aggcggcgga actggtggta cgattacagg aagctggggc aatccattcc
    24841 caggaagtag cttagataaa aattcgttca gtggaggaca actttttggg aaaaatccag
    24901 gaggagaatt ccgacctaac gggttccatg atggactaga ctttggttca gtcgatcatc
    24961 caggaagtga gattcatgcg gttcatggcg gaaaagtcgt gtacgttggc aatcctggta
    25021 tttcaggttt gggtgcgtgt gtgatcgtga ttaattacga tggcttgaat atggtgtatc
    25081 aagaatttgc aaatagtaca ggaaattctc gagtgaaagt aggcgatcaa gtgaaagttg
    25141 gtcaagtaat tgggatacga gatacagcac acttgcattt aggatttact cgaatggatt
    25201 ggcgtcaagc acaaggacat gcattcattg atgatggtac atggatcgat ccgttaccat
    25261 ttttgaatag cagtaaaaaa tagatcagga gaaaaaatat ggaaaataaa acgttaaaaa
    25321 tcgtcttaac agctgcaatt agcgttagtt taatcggcaa tattgcgttg ttttggcagc
    25381 tgaaagagga acaagcagca aatacctata aaattgaaga agtggctaaa aacgcgtctg
    25441 agaagctcaa aaaaaattcc tctgaggcac aggcgaagat cgacaagtta aatcaaacca
    25501 tcaatgagaa aaaccaagaa atcgatcagc tgaagcaaca agatggcaca aaagcaaaag
    25561 aggaagtaac tgaagcacaa agaaagacgg cagaagagtt cggtcaattg gccattgata
    25621 aatcattaag cagaaatgaa ttaacggaaa aactaaaaga tgtcgcaacc caagaagtga
    25681 tcgacaaatt aagtcctaca gacgatgatc accaacatga tgattacgga agttactcct
    25741 ttacattagg tgacgtgcaa acttacgctc aaacaagcag tgtaaaagag gaacaaaatt
    25801 ttgtcgtatt tgttgattat acaatcggca atccgcaatt caaagatgtc aaaccacaaa
    25861 aaataaaagg tggattaact gtcactgaaa aacaagtaga tggtcaatgg aaagtcatag
    25921 actttagtta tttcactcgt taggaggaag aaaatgagta agaaaaaagg agaaagacaa
    25981 cgtttcaaaa aacgaaaaag ttatcttaga tggctgggaa tctttatgat tctcggcttt
    26041 ttgattcttg ctagtccgat ttatacgaat cagaaggtag atataccaga tgaggtaagc
    26101 acgaacgcct ttttaaacac gaaagagctg acaatggttt caaaagaaaa aggagcgaac
    26161 cagcaattag tttttgaatt tgcgatcgat gatgaagatc aaagcttgtt aaaagaactg
    26221 gctaatctta attatcgcgt agaagtaaag acagctaaag gggattatca agcaatacga
    26281 gccaaagtga ttaaggtgtc tgatgattat tttgcagtga agctaacgca tgtaccagaa
    26341 gaatatatcg ccatgcgttt aaccattatt cctgaaaaaa ttgatccaaa agtggatatg
    26401 caagaaccac aagatctgat ttattacgtg cacgaagata aggttaaaga tcaagtgaaa
    26461 gacgaagatt atgaacaaca cgcgatcaat tacaaagtga aaggatacaa gaaagaacaa
    26521 aaagcagctc aaaaacaaat ggaatctttc caagcaacca ttgatttgaa tgaagaatta
    26581 gtgaagaagc taaaagatca attgccttat caagtcgcag atgatcagga aaatactgaa
    26641 aacaagatta atagctacca gcaagaaatt gaaacatcga aaacgcaact gaaagatcaa
    26701 gaagagatca ttcaaaaact tcaagaaaaa attgatcgta tgacaaaaga aaagatttag
    26761 aaggacaggt ggagtgaaaa aatgaagcga acaaacttgg ctgacaaaga tactttcgct
    26821 gagcagaaag tattgattaa agcctatctc aaagttgtaa aagctaaaga aatatgtcaa
    26881 gaagtgaatt atctaacaca acaatatctg agttgaagaa tggtagaaag agtttagata
    26941 ctttaaaatt cagatcattt gaagctctat atgtagctgc taaaaaatat tttacagctg
    27001 ataacaaata aaaaacagga gtattttatg tgaaaaaagt atataaaaaa tgcttatgaa
    27061 tcagctatat caccttaacc atttgttaat gagatgggag aaaacattaa ccacgattct
    27121 aacgcaaaca tcaagcacat aaaacattta acactcctcc tttctggctg aagatttcaa
    27181 ccaaacagga agacagcgta tcaattatag catacacttt ttcttaaatt catttttcat
    27241 atgacaaaat atttccttct aatcgacaaa gttagaagga ttttttattg tctaaataca
    27301 caaataacct taatttttaa agagcataag gaaacgtgat atcactttct cacgaaaggt
    27361 gttaggtagg ctgtgcctac cacgtttcta agaaagataa atgatatcaa aattttggat
    27421 ttgtgtgtag atttgtgggt aggatgaaag cgaatgtggg cagtaaaata agcgtttgtg
    27481 tgtagcaaaa tcaaaaaatg tgtagggata tgtgtagcta cacatatccc tacacataat
    27541 aaatttggag gaaaatatgg acttattaat tagaaatatt gaagaaaagt atattaataa
    27601 aattgacaaa cgttgtgaag aacttacggt caagactggc aaaaaatgga gtcgaaatca
    27661 atacctaaaa gttttgatcg aaaatgattt tgatcacgct cttctgaatt ataaaaaaga
    27721 ccaatttgat cggttgttag aaaaatttgt ggatattcaa acttataata cgaaaacttt
    27781 agaagaatat attgcaacaa ataatcaatt aattggtttg ttaattgaat aataaaaata
    27841 gtattgtgaa tttataaaag gaggaaatca attttggcaa aagcaacaag aaaacatgta
    27901 tatctttacg atatgcatat cgaagtactt gatgaaataa tttctactca tgaagatata
    27961 tctaattata gtgaagcagt cagacatttg tgtatgatgt atgatccaaa aatcgaagaa
    28021 aaaataaatc caattcaaat taaattgaat gcaatgggaa aagaaatttc tattttaacc
    28081 gaaatggttt cagagtttgc ggatcttcat ttgaaagata caggaatact aattggtaca
    28141 aattcaactg tttatcaaga agcagaagaa cgtgtggaag gaaaaattaa atccaatcaa
    28201 accaaaaaat attcttctaa gttaaagaaa ccagtgaatg aatctgttca taccagagta
    28261 gatccatttg atccaacgaa gattctaaaa agataaaagt agaaggagaa cgtgatgaat
    28321 aaatcgcctg cagttattct tacttgtcaa tttaccttgc ccaataaaaa atctttttca
    28381 acatacattg attatatgac aagagaaaaa gcactggaag aaatggaacg acgtacgccc
    28441 gaagaagaac aggaactgca acgaatcaag aatgctttag aggattttta tattcctgaa
    28501 ggagaaactt tttcggaaag taagacggat aaagaattca gtgaaacgtc gaatgaagct
    28561 caatcgttga tggaacatgg aatcaatttt gacaagttag aggaacaaga ttttaccaaa
    28621 tatctttctt atatgacccg tcactatgcg ttagaacaaa agaaagaact gacgcaaagg
    28681 gaacaaaaag aataccaacg gttgaaccaa gcaatgaaaa aatatcaaac agagccacat
    28741 aaaaaagctt caggaaatct tcctggagtt ttttctatgg cttcagacga agtgaaagga
    28801 agtgatctga aggagatcaa acaaatcttc caaagaggac aacaaaaagg ctcaatcatt
    28861 tatcaagatg ttatctctca cgacaattcg tatcttgaaa aaatgggctt gtacgatccc
    28921 aaaacagata ccttagatga agaaggtttg aaagcagctg gaagaaaaat gatggagaca
    28981 ttattcgaga aagaacaatt gaatgaaaca ggttactgga tggcaacgat tcaccgtaac
    29041 actaagcata tccatattca ttttgcagtt gttgaaaaag aaaatacgcg taaaccgtat
    29101 actgagattg aaaatggtgt gagttatatc gtaccgaagg ggaagagaag acaagcaaca
    29161 ttagacgcaa tgaaaaccaa ttatgtacat gagttagaac gtttctctta tgaaaaggat
    29221 cgaatgatcg gtatggagaa acgaaattta ttgacacgaa aaagtgattt gcgtaacacc
    29281 ttaacaaaag tagtaaaaga accagcacgt tacgatcgac acgccatgaa cttattaaat
    29341 gaagtatata aaaccctacc tgaaaaacgt tcggaatgga attatggaga tgaaaatcga
    29401 accaagcttt caccatttac aaggggaaaa cttgatgagt taaccaaata tgttttaaca
    29461 aaagaacctg gatatcaaga atatatacag atttcaaaag cattgaaaga agaagccaaa
    29521 agcctttatg gggaaagcaa aagagaaaca aagaatgcag aaaaaaacgc cctctttgat
    29581 ttaaaaaagc gcacaggaaa tgcgatttta acagaactga agagacagga tagtcaaata
    29641 aaaccattaa tagaaagtgt caatcagatg caatctattt acaatgaacg atcctttcga
    29701 acactcaaac agttgccaac aaatgaatac aagtatagtt atcaagaatt cgtaaagcgt
    29761 aggaaacaat tccaacaaag agttgtcacg cagcgaagct tgcgtcaatt aaatcgaaca
    29821 attaatgcac atgaagaaaa ataccacgca agtaaagcat atgaagagca acagcgaaga
    29881 atccaaatgg aacaagaaag acaataggag gaagagaaat gaaacaatat ttagatcaat
    29941 ggaaagtcat agaaggatca ctaaaagaag aacgaatcga acaacttcct gattgtttag
    30001 aaaaagagca tttgtttcag ataagcgaaa tgttgagaaa cgaacagttt gatccaaatc
    30061 aatttttagt tgttgagtac ccagctactg gtgtgtattg ttgtaatcat gtgaagggtg
    30121 aaaaatattt tattattcaa gaatacgaag gtaagttagc gccttattat actacttggg
    30181 aaataaatga agagggaatc aataatttcc catgtgaaag cattgaagag tctatttctc
    30241 tcacggaatg ttaaataaat agagacgtaa aagagtcctg aacgtgattg tccaggactc
    30301 tttttcaatg aattgttttc cgccaattca ctgagtggtg cgattcaatt ttattatatc
    30361 aaaagaaagt gagattccaa agtgaaggga ggaagaagaa atgactcaga caacgattgg
    30421 tgttgtggtt gaaaaaagca gggataatct cgtttttgtg acagaaattc aaacaggtag
    30481 agcttttgta gtcacagaca aagctgcaaa agcgtatcaa aatggcaata ttttaacgtt
    30541 aaatctgact acgaagatgt tcgttgatgc agtagaaaat tatccatttg tttagacaaa
    30601 cgatatttta caaggaggaa acggacatgg cagaagaaaa agaaccacta gataatagtc
    30661 ggttgggaaa gtccaaaaga aaattagtac ggttgcaaaa cgaattaaat aatcaatttc
    30721 aaaagctatt tgatcaccaa aaattgacaa atgggcaacc aatgaatgac aaacgaaatg
    30781 gtcgttcatg gtttaaccag caagaaaaaa tagaaaataa aatacaaagt ttgagagaag
    30841 agatcaagca acaagaaaaa caggtagaaa aacttgaaag acaagaagag ctaaaggaaa
    30901 tgggctataa caagtacggc ggactggata tgacgatcga taatattcca agaatcaaag
    30961 aagaaatcga acgctttgaa aaaggtgaat cgactttttc agccgccacg atccgaaaat
    31021 atcaacggaa gttggaaaca ttagaacagc tgaaagaaaa atcagaaaaa ggaaaagaaa
    31081 atatcctccc tgaagtacaa gcgatcattg acagcggacg agtgacgcaa tggaaaaaaa
    31141 atccaaccat ttattttttg aaaggctatc gaaaagttgc tttagaactg aatgaaaacg
    31201 ggggatttga ggaatcaaaa aaataccaag ccacgtcgga agaggaaaaa gcagctgttc
    31261 gtgaattact tgaagaggct gcaccaactg attctctgaa ggagaaagaa aaagatcatt
    31321 tctatcgagt agaattcaac gaaaatgatc cagaaaaagg attgaaaagt tatggtgggg
    31381 agatcgtaac attagaattg atcgatcaac ttaaaacgtt ggataaccaa tacgctgcag
    31441 aagcaggtta ttataaattc tattttgaag aagtgaagaa cggagaagta gtcgatcaga
    31501 tccgaatgga tctaggggat ggcgtcaaca gtaatcaccc catctattta gagttagcaa
    31561 aattgtgcga acaagtacct gagaaggaac aaaaacaatc ggtcaaaaat gaacctgttt
    31621 cgaaagaaca aatgcagtcg aaagaaacaa aaacaaaaca aaagggctat caaaaacgaa
    31681 cggcagaaga gatcaaacaa gaagttaaat ccctttcgca acaggcactg gatgcggtga
    31741 aaaagcatac ccattcttct gaagacgtca aagaactttt gaactttatg tctcgttttc
    31801 cagaacgatc ctttagaaat caattattga ttgaggaaca atttccaggt gcaacggctt
    31861 gtttaggtcg tgccgcgttg aaaaaagaag gaatctatat caaaaaaggg gaacaaggga
    31921 acaagatctt tgtcagaaaa acggtcaaag gattttatga gaaaggacgt ggctttgttc
    31981 gagaaacaga agctacacca gaagataaaa aacgaatcga aagtggtcaa atcgaagtga
    32041 tcaaaaaa
//
LOCUS       DAZUKC010000028        31927 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000028 DAZUKC010000000
VERSION     DAZUKC010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.029"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(730..1071)
                     /locus_tag="ACG80T_001215"
     CDS             complement(730..1071)
                     /locus_tag="ACG80T_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS4840878.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1071..1301)
                     /locus_tag="ACG80T_001216"
     CDS             complement(1071..1301)
                     /locus_tag="ACG80T_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS4840879.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1588..1926
                     /locus_tag="ACG80T_001217"
     CDS             1588..1926
                     /locus_tag="ACG80T_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840880.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2131..2693)
                     /locus_tag="ACG80T_001218"
                     /pseudo
     CDS             complement(2131..2693)
                     /locus_tag="ACG80T_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(3425..4006)
                     /locus_tag="ACG80T_001219"
     CDS             complement(3425..4006)
                     /locus_tag="ACG80T_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4840881.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(4189..4815)
                     /locus_tag="ACG80T_001220"
     CDS             complement(4189..4815)
                     /locus_tag="ACG80T_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFS4840882.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(4837..6168)
                     /locus_tag="ACG80T_001221"
     CDS             complement(4837..6168)
                     /locus_tag="ACG80T_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFS4840883.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(6323..6724)
                     /locus_tag="ACG80T_001222"
     CDS             complement(6323..6724)
                     /locus_tag="ACG80T_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFS4840884.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(6810..7016)
                     /locus_tag="ACG80T_001223"
     CDS             complement(6810..7016)
                     /locus_tag="ACG80T_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840885.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(7111..8613)
                     /locus_tag="ACG80T_001224"
     CDS             complement(7111..8613)
                     /locus_tag="ACG80T_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFS4840886.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(8631..9086)
                     /gene="argR"
                     /locus_tag="ACG80T_001225"
     CDS             complement(8631..9086)
                     /gene="argR"
                     /locus_tag="ACG80T_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFS4840887.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(9184..9801)
                     /locus_tag="ACG80T_001226"
     CDS             complement(9184..9801)
                     /locus_tag="ACG80T_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS4840888.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            10022..11395
                     /locus_tag="ACG80T_001227"
     CDS             10022..11395
                     /locus_tag="ACG80T_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4840889.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            11470..12582
                     /locus_tag="ACG80T_001228"
     CDS             11470..12582
                     /locus_tag="ACG80T_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4840890.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(12654..14291)
                     /locus_tag="ACG80T_001229"
     CDS             complement(12654..14291)
                     /locus_tag="ACG80T_001229"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFS4840891.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(14459..15067)
                     /locus_tag="ACG80T_001230"
     CDS             complement(14459..15067)
                     /locus_tag="ACG80T_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS4840892.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(15451..16602)
                     /gene="queG"
                     /locus_tag="ACG80T_001231"
     CDS             complement(15451..16602)
                     /gene="queG"
                     /locus_tag="ACG80T_001231"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFS4840893.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(16621..17373)
                     /locus_tag="ACG80T_001232"
     CDS             complement(16621..17373)
                     /locus_tag="ACG80T_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840894.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(17514..18464)
                     /locus_tag="ACG80T_001233"
     CDS             complement(17514..18464)
                     /locus_tag="ACG80T_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFS4840895.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(18465..19304)
                     /locus_tag="ACG80T_001234"
     CDS             complement(18465..19304)
                     /locus_tag="ACG80T_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS4840896.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(19301..20047)
                     /locus_tag="ACG80T_001235"
     CDS             complement(19301..20047)
                     /locus_tag="ACG80T_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFS4840897.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            20285..21655
                     /locus_tag="ACG80T_001236"
     CDS             20285..21655
                     /locus_tag="ACG80T_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4840898.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(21710..22606)
                     /locus_tag="ACG80T_001237"
     CDS             complement(21710..22606)
                     /locus_tag="ACG80T_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS4840899.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(22720..22890)
                     /locus_tag="ACG80T_001238"
     CDS             complement(22720..22890)
                     /locus_tag="ACG80T_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840900.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(22887..23564)
                     /locus_tag="ACG80T_001239"
     CDS             complement(22887..23564)
                     /locus_tag="ACG80T_001239"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFS4840901.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(23655..24560)
                     /locus_tag="ACG80T_001240"
     CDS             complement(23655..24560)
                     /locus_tag="ACG80T_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFS4840902.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            24735..26189
                     /locus_tag="ACG80T_001241"
     CDS             24735..26189
                     /locus_tag="ACG80T_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFS4840903.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            26205..27236
                     /locus_tag="ACG80T_001242"
     CDS             26205..27236
                     /locus_tag="ACG80T_001242"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFS4840904.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            27243..28430
                     /locus_tag="ACG80T_001243"
     CDS             27243..28430
                     /locus_tag="ACG80T_001243"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFS4840905.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            28586..29968
                     /locus_tag="ACG80T_001244"
     CDS             28586..29968
                     /locus_tag="ACG80T_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFS4840906.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            30005..30325
                     /locus_tag="ACG80T_001245"
     CDS             30005..30325
                     /locus_tag="ACG80T_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840907.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(30525..31193)
                     /locus_tag="ACG80T_001246"
     CDS             complement(30525..31193)
                     /locus_tag="ACG80T_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFS4840908.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(31382..>31927)
                     /locus_tag="ACG80T_001247"
     CDS             complement(31382..>31927)
                     /locus_tag="ACG80T_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4840909.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSLVQFRKTTVTVV"
BASE COUNT         9002 a         6474 c         5770 g        10681 t
ORIGIN      
        1 gtacctaaaa gctctactga cataagatcg tgtaatcggt aaagtaattt tattaattat
       61 acgggatgta atcaattaga gaataaacta atcataaaag gtacttttac attcataatt
      121 gtcgctttga gattctactt tggttcacta tctgacccaa ttgcacattt tcgatcatta
      181 caagtattgg tgtcgttaga acaactaaat aaaaactagc ggtagatttt tcagaatcta
      241 tgctaatttc tgtatggatg ttccagtgat ggcaaatttc tcttttcatt gtttttcttg
      301 tcgcatgata tgcctttttg tgttattcag ttaaactata gaaattcttt cctagacaga
      361 tcttgatccc aaattttacg tttttgagca tcgatatagt ttaaaatgac attactcata
      421 tctaaagagt ttagattttt taggactaaa actgtcagtt gttctttttc ttctttctgt
      481 taaagtattg tcgcgacttt tcgtatacgt taagatatgt cgtgaaaaaa caagttttgt
      541 gacacaaaaa aacgaatgac ttgaggaaat cacctcaatc attcgtttaa aatgctatat
      601 attctttagt ttaagatacg gaaattaatg ccatcgacca cactagtaga atgaacgaca
      661 tttacttggc ctttttttaa tttcgttatg tttagcatat aaaaatatta tagagtttac
      721 ttacaatgct taaaacatat actgtataat ctgatcaatt ttagccatgt cttgcaatgg
      781 taagttttct atttttttga gttttcgttc cttaaaatca agagatttta attgtgtaat
      841 aattacttgt ccttttatat ctagctcttc aggtaatgag taacgtgtcg gtaaattctt
      901 tatagtggaa gtaattggac atatgaccga aaacattgtt ttacggttaa attcataacg
      961 agagacgact agtcctggtc gtcttttttg aatttctttt cctacggagg gatcaaaatc
     1021 aatccataca atatcacctt ttttaggaat ataaattctt tcttctgaca ctataaaatt
     1081 tctctccctt caggagttaa atcttcccat tcgtctttct cataatactc ggcctcttcc
     1141 ccaccactaa atggatcttc aatttttgga actaacgtaa ttgttccatc atcagaatac
     1201 acaacgatat attcttggtt ttctgaaggt ttctttccgt tatcagaagg taaagttacc
     1261 actacagaac ttccttgtaa tctagatttt gtaacaagca ttatcatcac tcccttaata
     1321 aaagtatact atataacact aagtaacgca aaattcccct ttgtaaacta tctagaaaat
     1381 tgttttgttt aaagtatatc ttattggata taaatagaaa taaattgtag ccagttcatt
     1441 ttttctttaa ctaaaaaaat aatccaagaa acattgaacc tatggatcca aaagctaccc
     1501 aaatactata gctaaccccc cgctaccttc aattttgcag ataaaacgct ttgggagaaa
     1561 atttcaaaga caacaggcca tcctatcatg cccgattata aacaaaaagc tggctattcc
     1621 ttttgctctg acaaatttca attgggaaag ttcacgaaac tagcaggtga agaagtttca
     1681 actgttcgga tcaaagaatg cccgcttcaa atagaaacat tggttcaacg aatttcaact
     1741 agacaagact ttgctattgt agaacgtgag atcgtagcta tttttgtaga agaagggatt
     1801 ttacatgatc caactcatat caatgtggat aaatggcaac ctcttatcta taaattcaga
     1861 gaatatacaa ctgccaacca gtcgttaact ttgaatttta gattccaaga attcaaagag
     1921 tcataatgat aaacatcgtt aggtataaat aaaaaagact cgcagcacga gtaaaaaaca
     1981 aatagaagct atacattaga aaggtcgact gtgaccctat agatgcagta tatgcagctt
     2041 atgagcattt cttctgttct ctttgttttt gggatctgtg ctgaagtctt tttcgttttt
     2101 tgccgctctt tctttgaaaa atcgattatg ttataccttc tccattacta attcaggcat
     2161 ctgctgagta atgatttttc gcgcaaaaga aatatctgtt acccaaggtt cgatttgttc
     2221 ttcagtcaag acaagcggca ttcggtcatg gattggtgcg actgtgcggt taggttcagt
     2281 agtcattatg attgattctg ccattttttc atcctgatgg ctacgataaa aaccgccaat
     2341 atacactatt ttttccttat ttgaaaacat aaatttgtct ttttcttcat tccactcatt
     2401 gggaaaacac aacgattttg caaaaatggt tgatgaaagg tctttttttc ttctactgtc
     2461 tctgcacggg cattaatcat caattgtcct tttttaaatc cggtaaatcc ccacttcatg
     2521 atttccggag tgaggatgtt ttcttcctct tttcttaacg tcacaacaag atttgacgga
     2581 aaaacttctc cagctgctaa ttctgctgcc tgcttcggtt gtttcgtctg ggcttctcga
     2641 taataattct gaaggagctc tgactctaga tcaaaaaaat aacgaccaca catcctaatc
     2701 cctcccaatc tttttttcta tcatacactg caacaagaag ttcgcaccaa caaaacgctt
     2761 tcaatttttg tagaactaaa aaagttgtga caagtgacaa actctccagt gaatggtatt
     2821 ctcctgccaa ctcctcggtc acaagtcaca atattcaaca aacatgagaa gctgcttttt
     2881 gaaattgttc cttgtgactc ggagtgaaac ggcaggctta cgacctctgg gtaaacggtg
     2941 ttcaaaagcc agtttctcgg tattaagcca aatttatgca aaaatgtaga aagctatttt
     3001 cacaaaattt ttcttaatac tcgaaactaa gcgacttttt cacaacctct tccttaaacg
     3061 gtgttcaaaa gcttgcatct gcggtaataa gcccaaccaa acgaaaaata tgagcagcta
     3121 tttttatttg gctgttctta ttacttgatg cagggcagct ttttcacaac ctctccacta
     3181 aacggtgttc aatcagctaa ccttcggtat tagctcaaaa aacggcaaaa tatgaggaac
     3241 tattttgccg tttgtttctc taatactcag gtcaaagcgc tgatttcaca acctctaata
     3301 cacggtgttc cagaagtagc ttcttcgaaa aataagccga aatttcacaa aaatttgaga
     3361 agcaattttc gtaaattcct tcttatttct tgaagctgac cacttctgtc acaacctcta
     3421 attattaaag aaccttttgc gaagttaaca atctttcttt gatcaattga aaattctgcc
     3481 taagtagtga tagatcctgt gtgactttca acaataagat acttccctgg atttcagcaa
     3541 tcaacgcttt ggcttgacgg tctgcttctt catcggacag tttttcttca gaaagtgcca
     3601 ggatattttg tttcgtatac ttgacccatt gattgactaa ttctagtagc aacagacggt
     3661 attcttcatc cactgtactc aactcaataa tcaaattacc tacaggacag ccatgataag
     3721 tgatctgtgt ctcatgatca gcgaaaatcc agtccaacat ttcttctaat gcttcttctg
     3781 gctgttttcc agcatttaag atgtcttcga acaattctgt ctgccatacg tccatatgcg
     3841 catggatcac cgctagacaa acttcttttt ttgaagaaaa gtaataataa aattgacctt
     3901 ttcctgtatc agaagctgct agaatatctc ggattgacgt ttcatgatag ccttttttat
     3961 aaataagatc tttggtcgct tccaagatct tttcctttgt tttcaatgat attctccttt
     4021 ttcatttctc atagtcctat attatacagt aaattcatca ttttttcttc ttaatcgttt
     4081 aaaaaagact gtgccaaatt atctgacaca gccctcacta taaaactgtt ctcatatagc
     4141 tagatgttca ttgattcaag aactgcgagg acaaaagcaa ttcctatctt atgatagttg
     4201 ctctaatact tcctctgttg tcatcacttg atctgcaata aagccaaagt tcgtcaacgc
     4261 tgcatccatt gcttcttggc caggagcacc aattgcatct gtcgctacca caacattgaa
     4321 tccactctct accaattcac gcatatggga atcgctacac aagtttccgt tcattccacc
     4381 aagaattact gtgtcgattc cattttttct caattggagc gccaaatcat tgctttctgg
     4441 tccaaagatt ttatgaggag agctgatgat cgtattttca tccaaataag gtttcaattc
     4501 ttcgataaaa tcggctcctg aaccttcttc cactgattca tattgacttt ttctagcaaa
     4561 catctgctgc tcaagcatca tatgttctcc tgcaccgcca aattgccatt tatggtcatg
     4621 tgggtaaaaa taatgaggag aaataaagag tttgtatcct ttttcttttg cttgttttac
     4681 catttcaatc atattttcga tcgtattgta tttttctaag atatcttttg tcaaaccaaa
     4741 gccaccgcct gctggtttca agaactcgac ttgcgggtca gtgagaacta ctgctgtatt
     4801 tttattattg aacatggtgg attctccatt cgttatttat tttaatacat catttagagc
     4861 ttcactcatt gtcggatgag aatagatttg atctctcaat aacgtataag gaagttttcc
     4921 attcatagct agcgaaatca agttgatgac ttcatgagaa tcttccgcat agatacttgc
     4981 ccctagaatc aagtcagttt cagggtcgac taaaactttg aatacacctt taggatcttc
     5041 taatacttgt gctttaggga caccgcttgt catatacttg aataatttgt aattgacgcc
     5101 ttgtgctttg gcttgttttt ctgttaaacc tacattggat aaagtcggag tgataaatac
     5161 actgtaaggc acagctttcc ggttattcgt tgttcgttta ttctcgccct tcaactgatc
     5221 cataatgatt cgataatcat ctagagagat ataagtgaac tgcaatccgc ctttgacgtc
     5281 tccaatcgcc caaatgttgt ctgcatctgt acgaaggtat tcatccacag cgattgctcc
     5341 gcgatctgtt agtttaacat ctgtattttc taatccaagt gatgctgtac ttggttttct
     5401 tccagttgca gccaaaactt ttgacgcttg gattgttact tcttggccat cttctgtaaa
     5461 ggtgacggca gctgttgatt cttggtctgc tacttgatct acagatacac ctagatggaa
     5521 ttcgattccc gcatttgtca tgtcttctaa aatcatttgc gcgatatctt catcttcacg
     5581 tggaagaaat tcagaatggg catcaagaac gacaacttta gatccgtatt cattgaacat
     5641 tgatgcaaat tccaagccaa tatatccagc cccgatgatc actaattttt ccggtaattg
     5701 ttcctgatcc atcgcttgcg tagaatcaat caagtagcga cttgtattca atcctttgat
     5761 tggtaaaatg attggttcag aaccagtatt gatgaaaatc cgttcgcctt caacaatcac
     5821 tttttcacca ttattcgtca cttcgatttg atggttagaa agaaaacgtg ctgtaccgtc
     5881 catcactgtc ccagttgcct catcgctgat catatgataa tttttggcgc gaagcatgcc
     5941 cgtcaatttt tctttccgag caacagcctc tgtaaagtcg attcctcttt ctccattgaa
     6001 aatcaatgat ttagaaggaa tacagccgat gttgatacat gttccgccat acatacgtgt
     6061 tgacttttca acgactaaaa ccgattcacc ttttgaagct aacgccttcg ctaaagtctt
     6121 accgcccttt ccaaaaccga tcacaatatt ttgatatttt tccatcattt ttccctcgct
     6181 tcttttttac ctaccgattg gtacaaaaat atgataaccg ataattgtct attctccaaa
     6241 tcatctgctt tatttgtcaa aaaaaaccag acaaaaagcg gtcctttcac gtttttagtc
     6301 tggttcgatt tcctttattc ttctaaacaa tcgttacttc tacatcgata tttcctctag
     6361 ttgctttgga gtaagggcag aattcatgcg catcttctgc aattttttgg gcttcttctt
     6421 cgctgatgcc atcgatatgc acttctaata ctacaccgat ctgatagccg ccatcatcta
     6481 aattataaag agagacacgt gcttttactg tggactctgc tgtcattcct gcttctttga
     6541 tcatatgttg taccgcccca ttgaaacaag aagcataacc tgctgcaaat aattgttctg
     6601 gatttgttcc ttctgcatgg attcctggag gagtgatttt catatgcatc gagccatttg
     6661 gtgcctcgac ttctccattg cgcccgccat ggttgatcat tgctgtttcg tatgcttttt
     6721 tcattgtact gctcctttcg attgaccatt tgctacttgg ctataattat agtgtatgac
     6781 aagtaaggcg ctttctacaa aaattctgct tattcgaata ccagaatgcc gtcttctact
     6841 atttttccaa aaaacaaaaa aatactactc gtgaacaaca gcgtcatatt tgttccaaaa
     6901 taaatagcaa tcttttctac ataatctttt atatgattga ttttaagata catatatacc
     6961 agaaagttga ccccataaac caatactgtt aaaaaaataa ccatattcgt taccattttt
     7021 tcctcctttt gctggtctcc ctaccagcac tcgatttaac cataccactt tttttgccgt
     7081 ccaagcaact agcctttcaa aaagactggt ttattctgct gttaatgatt caagatcaga
     7141 aaaatgtccg aataattttg ctaattttac taacgccaac ttgttctttc cgaattcatc
     7201 attttctatc tgtaatacta gtaacggctc atgtaagctt aaacggatca aaaaccagcc
     7261 gtttccgtat ggctcttcta gatcatatcg aataccttct tcattttccg ggtcgaagtt
     7321 catccctttt tcttcttcca acagtcgctt catttcttct aatatcttgt ttccttgttc
     7381 gcgtggttca tcgctgaaca attttaatcg gatttcttgt acttctacgg gctgtttcaa
     7441 ctctttgatt tgttcatcta atgtgatacc ttttcttttt aattctggca aaagcatcag
     7501 gatctttgcg atgacatatg caccatcatc cagaaaatag ttttccttga atgcagcgtg
     7561 tccgcttgtt tcgatggcta gctgtgtatc tttcccaaga cggttaagtt ctaatgcttt
     7621 attgattaca tttcgatagc cggaaatata acgaacttgg atgcctcctt tttcttcaat
     7681 gaatctttgt agatgagaag aagtagggga attcgtcacg attcttgttc ctggatgctc
     7741 ttttaatacg attgtactta atactgcgat caaattattt cgattgatca ctgttccaga
     7801 agcactgaca actgcagaac gatccacatc tgtatcgaaa atgatcccaa gatctgcttc
     7861 atttgctaaa acagcctgct tgatactttc catcgcttct ttattatcag gatttgggat
     7921 atgattagga aatcggccat ctggttccaa aaattgggaa cctgttgaat ccgcacctaa
     7981 gctattcaat actttttcag caaagaatcc accagcacca tttccggcat caacaatgat
     8041 tttccaacca tttagtggtt tgctttctag tgttccaatc ccctttttaa tttttgtgac
     8101 taaatcttgc gcataaggtg tcaaaaggtc tgctttttct acgcttccag tttgttcgct
     8161 ttttattttt tgctgctgtg ataggataaa agcaatatct tctttttctg ctcctccttc
     8221 tgaggtaaag aattttattc cattataata atagggaaga tggctagcag tcagcataac
     8281 cgcagcatcg cacgagaact gttcaaattg agtggacata aacatagcag gcgtagtagc
     8341 taattcgaaa tcgatgacat gtatcccttg ttctttcaga ctttggatca acgcctcttt
     8401 cagtgctggt ccgcttatcc tactgtcatg accaatccca atcgtccatt ttttatttgg
     8461 atcggttgat ttcttctttt tctttatcca gtttgctagt ccccagccga tcttttttac
     8521 catgtctgtc gtcaagttta cttcatatcc ttcaactggt aatgcgattc ctcgaatatc
     8581 cgatccattt tgcagcgctt tcatatcttt catataaaag tcctcctcat ttattccctc
     8641 attttttcca gcttttctgc aaatcgctgt gcttcttctt ctgagcggca aataataatg
     8701 aatgtatcta caccggccat cgttccgaca acttcagggt aagccacttc atctagccaa
     8761 ttcgttacca catcggcacc atttcgttca gtcaatacga taaccatgaa ctgaacagac
     8821 tggatacgca aaacctcgcg tctaattgct gtacgcaaac gttcttcatt taaagaaaca
     8881 gatggctggt taaataatgt atagcgtact attttgtttt cattttgact ttttactaac
     8941 ttcatttctc gaatgtctcg agaaatcgtt gcttgagtca catcggcgcc tgcttcatgc
     9001 agccgcgcaa gcagctcttc ctgtgtccca ataggttgtt ctgtaatgat ctggcggatc
     9061 agtgcttgtc gctgtttctt tttcatatta gacacctctc aatgttgatt atagcaaaag
     9121 ataagtaata tttcttcata ttttggtatt ttttactttt attttttgtt ttcattcagg
     9181 ttgctaacct ttttctattt taacacgcaa aatcgtcttc attttttcag gtgctgattt
     9241 tcttggatcg ctgatgtaaa tctctttgtg ttctttagaa gctctttcat agccttcttt
     9301 tgcgatgaat gcttctaatt tctcaaacgt tgctggttct tcggcatagg gcccgacgtg
     9361 taagatttgg gcacaaagac cttcttccat ctttttgaat actacttgtt ctttcaagtc
     9421 ttccggaagt ttgctagaac gctgaatggc ttcttgtgct atcgcttctg taacaaagtc
     9481 aggctgtcgg atcatcaatt gataagaaaa atgttctttt ttcatcactg gttcatgcaa
     9541 atacttttct tgcagtcccc attgtccttc cagtggatag acagtataca tctgataatc
     9601 aggaatcagc caattttttt taggcgacat gcgtattgca tagctgactg catatagaca
     9661 ggctactctt cgtttaaagt cttcctgatt cgggtcgccg atccctctga tgctaaagaa
     9721 tgatttttta ggaacctcta gtatttgcgg gctttttttc atactatagc tcttgtcttt
     9781 acgccattca attttttcca tttttcttcc tccgcgaaat tccatttagt tatttattat
     9841 catacctctt tttgagtgac ttggctaatc gtgttgacac tatttttcag tttgtgctag
     9901 aataagtgaa gtaaataaat cgtacagata acacgtttcg gcgtgtcgag agagaggcgg
     9961 tttctatttt tataatagaa atcactctct ttttttacga aattttttag aggtgatgta
    10021 aatgacaaaa caaaaacagc tgcttttagt acctgcagta attgcaacag ggatgatgtc
    10081 ttttgcgggc gtattgatcg agacggccat gaatgtaact ttcccaactc ttatcagaca
    10141 gtttgattta acgactgcac aagtccaatg ggtaacaacg atttatcttt tgatgatttc
    10201 gatcattgtt ccgctgtcta cttatttgaa tcgcaatttt agtctcaaaa aactcttttt
    10261 agcttcaaat ctgcttttct tggcaggtat aatgatcgat ttcttctctc ctaccttcat
    10321 ggtcctattg tttggtcgat tgctccaagg tgcagcgaca ggtatcgctc ttccgttgat
    10381 gttccatatt atcttgacat ttgcccccct caatcgacga ggcgcgatga tgggtgtcgg
    10441 tactttgaca acggccattg ctccagcaat tggccccact tatggaggca ttatgacttc
    10501 acattttaca tggaatcata tcttcctgtt tcttgtgccc gttttgtttc tttctttgat
    10561 tatcggctgg tttgctattc ccgagatggc agtacaaaaa agtggttatt tggattggat
    10621 aagcgtttta ggaatttgtc ttttgtttag tggaagtcta acatttttga gtacgttggg
    10681 aacagtttcc agctggattg ctttaatcat cggcggtatt ggctggttcc ttttttacta
    10741 tcgaagtaaa aaagtggagc atccactgat ccgactgtct attctttcaa ataaaacatt
    10801 ccgcttgttt ttgattggct tcttagttta tcagttttta ttacttggtg tatcttttgt
    10861 cttgccgaat ttccttcaga tcgttcaagg aagtagtcct tttgtcgctg gattagcgat
    10921 gcttcccggc gcagcggttg gtgctttgct ttctcctttt tccgggaaaa tgctggatca
    10981 atatggtccg aagaagccaa tttttgctgg gctgattctt tctttgattg gttggctagc
    11041 attattccta ctcataggac acgcttcagt gggtgtattg attgcttgtc atgtgtttta
    11101 tatgatcggt atcggattat cttacagcaa tatgatgacc accggaatga atgcactgaa
    11161 tgaagaacta caaggagacg ggaacgcaat gttcaatacc ttgcagcagt tttctggcgc
    11221 tgtggctact tcattagtag cggtcattat caattacgtc cagcatcata cttcccataa
    11281 ctatgaagtg tctactacat taggatcaaa agcagcatta ggcgtcttgt tgctgttgct
    11341 tctcgtaagc tttgctcgat tcgcctatta tctgttcttt gctaaaaaag cgtagattcc
    11401 atatacggca ggtgttgcat ctgccgtttt tttgtgtgaa aatggttgtt tgtaaaagga
    11461 ggctttttta tggaaaaaat tgccattatc ggtggcggga tcattggtat gaccctagcc
    11521 agttacttag atacatcaaa attcaatgtt gctttatttg atgaagggac aggacaggca
    11581 actaaagcta gcgctgggat tatctcccct tggttgtcga aacgcagaaa caaacaatgg
    11641 tatcgtctgg ctaaagacgg cgctgctttt ttccctaaac ttataaaaga tttccaactt
    11701 gatcaaacga tttacgcaca atcagggaca cttttgcttc gacctgaaaa agatttagct
    11761 gaattggctg ttcttgccga gaaacgaaaa gaagaagcgc cagaaattgg aaacatcgaa
    11821 tggctttcac ctgaacagac atctcaagca cttccgttat tgaaaccagc agtttcactg
    11881 cgtatcagtg gtggtggtcg tctggatgga aaagcatatt tagaacatct tggtcagcgt
    11941 ttagcacaaa aacaaatgga gcagatccag gcaaaagtca ctttgcataa gaagagcgat
    12001 tcttggctca tcaagggacc gtttggagaa tacacagctg atcgtgtaat tttgacacct
    12061 ggcccggcac ttaagccttt attacaagaa ttaggctatg ctgcagatat acgtccgcaa
    12121 aaagggcagc tcttagcatt tcagacatct tataagatga gtcaagactg gcctgttgct
    12181 atgcttgacg gagaagctga tttcattcct ttcatggatg gaaagatcct tttaggtgct
    12241 acccatgaaa acgatcaagg ctgggatttg gctcctacta gcgaagctta taagggattg
    12301 actaagagta cagaacagtt cttgactgat ccagatcaga taaaaaactg gccatacgag
    12361 ttccgtgtag gtacccgtgc ttatacgtca gatttttccc catttttcgg cgtactgcca
    12421 gatgatccaa gcttactggc tgctagcggt ctaggctctt ctggcttgac cacaggtcct
    12481 tatatcggct atctattagc caacttctta aatactggtc attggcctac ggaagattat
    12541 caaaaggaca tctccaccta tattacgaaa aatccatcat agaaaaacag ctttttcaag
    12601 taaggatccc gagcgatatt gcgcatggat tacttcttga aaaagctgtt attttagttc
    12661 aatttctgaa tcaaattctc caaactttct tttgcttcat ctggcaagag atcttttcct
    12721 atctgagctt caatgaatcg taaacgaata tcaagtcgtt gtttcaccag atttttatag
    12781 gacgtatcct caaaagaaac aggaaaatct ggtataggta aatagtggat gtcttcaaat
    12841 ggaccaaacg acttgaattc tgccgtttgt tgcacgattc tttcaacgat tgacaatgtc
    12901 gtttcaggtt tgattttttt atcatcgaaa tatccagtat tcaggacata gcaggctgtt
    12961 tgctgtttca aaaacaactc tcgaaaagct tgatagtctt ccctaagagg atagacacgg
    13021 aaaggattag caaatggttc aatgactagc tcatctagct gtctttttgt agtgatattt
    13081 tccgcgtctg atcgttttgt tgctaatgtt gcaccaaaag acgctgctaa aactgggtca
    13141 ttgatcttga taattggcgg aagcgaatca tctttcatga tccaaaaaac agcatcgatc
    13201 ttttcgctaa gatgatcgac tcggtcgggt gtcgcataac gtgatttgac tgtccgccca
    13261 tttccgtttc gtatatctcc tgttactagg actttttctc ctttacttgt acatgtcacg
    13321 cccacattct gacaagtaag gaaagaagaa atggccggat catcagacgg atagtcttgt
    13381 gttttatcaa agtaagaagg ttccaaggca gtagcaaaac cattttttcg agaaataacg
    13441 aaagcatcgt catgcaagat cgaaacagag tgaccttccg tatgttttgc taaggtaatc
    13501 gttgattttc cagaaccgga caacccaaat acagccatcg tataggtctg gtcattacta
    13561 gcatattgtt tcatcccacc gtggcaggca ataaagccat tgcgatttcc caatgcccaa
    13621 gcaagcgtca aagtcgcttt ttttaattct ccaaagtaac gtaatcctag cacagccgct
    13681 gtattatgga gagcatcaaa caaaatgagt ccatttggaa atgtttcatg tgtccattcg
    13741 ggatcacaaa agatataaag atctccctca tcaaaggaaa cagacttttg ataagtctct
    13801 aagatcttcg aattcgctgg ctgaaaattc agcaaataag aatacaaatt gttctcaaat
    13861 ccttctggaa tcagcaaatg actttgcaca gaaaattctt cagataggcc aacgattact
    13921 tttgtatggt aaaactggcg cttgcgactt tgatacacag cttctcttaa aattttctcg
    13981 tatttgcttt gttccttgtc taagtcctca aagatccgcc gagcacttgc tgttcttccc
    14041 acaatgcttc catcattgaa tactagtgtc ctaacatcag ctggtaaatc tagttcttca
    14101 gcatgtgaga ttgttaaatc tgtgatgatc gttcctggac tcccttttgc tagttgataa
    14161 gctgtcttcc tatcaaaaac ttcatgaaca ttattaccgt aaaatgtcgt ttctacaatc
    14221 gatcgaatac tggataacag tggatttcca cttgtgattt cgtttaattc gagactctct
    14281 tttgtactca tggctcctcc tcattcccta aacaaaaaaa cgttttcttt attatacatt
    14341 attcgagttt ttatttgtac tgctgcgctg tgggaagcaa acgagttatt tttcacacaa
    14401 aaaaaccgag aacttgaata aacaagtttc tcggtttcat cattttcctt ttatcggctt
    14461 agtaccagcc gtttgctaac cagaatgttt tagcagcatc ccatgaacca taacgagatg
    14521 ttacatattg ttctgctaca cgttcttgat tggcagctga atagtcgccg tttaagtatg
    14581 aagcatctaa ttgataacgg ccgatgtaac gtccgttagt agctgtgtat gaaccgcctg
    14641 attctttttg tgcgatccat tcttttgcag agcttgaagt tggcgctgtt gcagcagctg
    14701 gggcagcagg agctacttca actgtttcaa caggtgcaac gtatgtatgt tcttgcgttg
    14761 ctgtttcagt tgtagatgct gtattttctg ttgttttgtt ttctgttgtt gctgaagaat
    14821 catttgaagt tgggattgtt aattgttcac caacataaat gcggttgatg tttgcgatcg
    14881 aatttttttc agcaattgca tcgatcattg agttgtctcc tgcaaatttt tgtgagatct
    14941 tagataatga atcacctgat tttactgtat aaacttcatc agcgtgagca tttgtagttc
    15001 ctacaaacat tgcagtagca ccagcagcaa aagttgtacc taaaacaatt ttcttaaatg
    15061 aattcatatt aaactccttt ttttctccgt tttttattct ttcgacgcta ttaactttat
    15121 catttagaaa tatttcatag gtaaaagaaa catgacattt aatgacaaaa taacgaatta
    15181 tttaatattt gtaacattta aacaaatatt tttttgatac aaaaccataa aagccttgtt
    15241 gtataagcaa agtaatcgtt tttctatgtt tttttatttt tgaatgaaaa acgcagattc
    15301 tgtaacattc actgcaataa tccataaaaa aaagcaaaaa aatttttgat ttttatcaaa
    15361 ttctcttaaa aaaatgtaaa aaatacaaaa aaaccagtaa ttgttacaac aattactgga
    15421 caaaaacttt gaataatgtt aactattttt ttattttaac tttctccgca ataatttctg
    15481 ggcatgttcc aattcgttta taacgacttc ttctgtttct ttagctaata tttcatctaa
    15541 tacatcaagc catttttccg gttcttttgt tcctagacgt ccaatagccc aagctgctgt
    15601 tcctctgatg acagggcgga catctgttaa acattctgaa atctcaggca atgccgttcg
    15661 atcacctaga ttagctaaag caatcaatgc gtttctctgc aaaggtttct tcccacgcca
    15721 tgaaccagct aaatgcccaa attgctgttt gaagtcttta ttagacatcg aaagcattgg
    15781 ctttaatttt ggatatattt cgtctacttt tggttccatc tcttcatgaa aatgaaaatc
    15841 ttttccttga ttgtaagggc agactaactg gcagatatca catccataaa tgacatttcg
    15901 cattttttta cgatattctt ccggcatcat cccttttgtc tgagtttgat aagacaaaca
    15961 tcgctgggca ttcattcttc cgtcaccaag caatgctttt gtcggacaag cagtcacgca
    16021 gcgcatacag tcaccgcaac caaattctcc tggtttatca ggctcaaata caatattagt
    16081 aacgatctca ccaagataga caaatgaacc gaactcttca gtgatcaaca atccgtttcg
    16141 tccaataaat cctaaaccag ctcgctgtgc tacagcgaca tcgatcagtt ctccagtatc
    16201 tacttgcgga gctagacgcc attcctcttc tttttgccat ttttctgctc ttgtttggat
    16261 gaattcgatc aatcggttca aacggtctct gagtatatca tggtaatcga tcccccaaga
    16321 agcacgagca aactgcccgc gtttttcatc cctaggcact tcttcatgga tcctagtagg
    16381 atacgcaagt gcaatggaaa tgatcgattt cggctgatca aatgtcattt ctggataaag
    16441 acgctcatcg atatttggat gttcaaagcc agaagtatgg ccagctgctt tttgttcttc
    16501 caacgattct tttaatgaat caaaaggttc cgccgtggtg aacccgattt tgtcaattcc
    16561 taatcgtttg ctttcttgta tgatttcttc ttttaatgtc atggtcttcc tcgcttgctg
    16621 ttaacggctg tataacccat aatatagttt ttcttttttc agctggacaa actggatctg
    16681 ctgatcggca atcttctttt cttccatcca tttttttctt gcagcaaata cttcccaata
    16741 atttgttgaa attaattttc ttttgttatc taatgcatta aaaaaattca atgaaactgg
    16801 ttttgtactt aacagttcat ggagccttac ctcttcccat ggcgaaaact cagacagatc
    16861 gcgggaatac aatacttctt catagagatt tttttgctgt gtgtacgtga atgtttgcgg
    16921 gattttccca ggcctagcaa tttctactag acgattataa aacatattca ttaatttgct
    16981 gttggcaata aattgacttt tataataggc cgactggatc tttttgatta ctccttctgc
    17041 tttgatgact gaaaataatg caggccatgc tttgacggtt atttcgtcgt tttgtttaac
    17101 taatacgcca ggtgtgaagc cgactggaga aaggaacaca aaataaccga taccggtatt
    17161 ttcatctaaa ggctgaaatt catcaaacaa ccatttgcct atgtacagct cttcatctgg
    17221 ttctgttgtc tcattttgat ttgctatgaa taatttagct aattctgctt caagaaattg
    17281 aaggtcttga tccttgtcgg tattttcctg ctggctgatc aatcgaaaga cttcttcttt
    17341 aatcgtatat ttatctttac gtgtaaaatt catagccaca ccttccttcc ttactctaac
    17401 tataacggaa tttcttcaaa aaaggtatct gtacgacgaa aaacagcata attttttttc
    17461 atatctctta taaaaaatgc aaggtctgct cctcaagcta gaccttgcac ttttcagctt
    17521 tcttttgttt gtgacaaacc ttcatggatc gtttcaatat tccatttcat catctgatag
    17581 taagaatctc cttgttctcc ttcttttgct atggaatcag tgaacagttt atcatagatt
    17641 ttcaaacccg tttcttttgc tacccgttcc atgcttcttg agtctacact ggtttccacg
    17701 aataaaaccg gtacttcttt tgctcttatc tgatcaatga tcgctttcat ttgatcaggc
    17761 gtaccttgac tttctgtatt gatctcccat atataagctg ctggcaaatc atatgctcgt
    17821 gaaaaatact tgaatgcccc ttcacttgtt actaataatt ttttgttctc ttcgatagaa
    17881 gcaaaacttt ccttcgcttg tgtatctaat tcttttagtt tttccacata tgcttctgca
    17941 ttttttttat agagtgtcgc attttctgca tctttttcag agaatatacg tgcgatttcc
    18001 tccacatatt ttattccgtt agataggtct agccaagcat ggggatctgc ttttgtatgt
    18061 tcttcaccgc tagttaaata aagaggttct acatttttgc taactgcaaa ataatctttt
    18121 ccttcttctt ttttagccgt ttccatcaag ttatcgaacc agctgttacc tgtttcaaga
    18181 ttcaaaccgt tgtataaaat aacatctgca tcgcttgcct ttttgatgtc ttctggtagt
    18241 acttcatatt catgcggatc tgttccgact ggtacgatac tgtggatatc tatttgatct
    18301 gttccgacct cttttgccat gtccgccaag atcgaattgg tagccacgac tgacaacttt
    18361 tctttacttc cttcatccgc ttggtcagaa gcctgctttc cgcaaccggc taataccaag
    18421 aaactaactg ccaagattaa aaagagtgat ttttttgttt tcatctatct gcttcctttc
    18481 tgaaataaaa ttccttttgt tggagaaaat aaaaaagcaa ggataaaaaa tgcggtacat
    18541 gctaacacga tcgtagctcc tgatggccaa ttgtaactat aactgaaaaa cacaccgaca
    18601 actgcactta gcataccgat tcctgcagct gtaatgatca ttttcagtaa atggttcgtt
    18661 agcaagtaag ccgtggcggc tggtgtgatc aacatggcaa tcaccaagat tgttcctact
    18721 gtctgtaaac tgactacagc cactaatgtc aaaaagaaca tcaaaagata atggatcagc
    18781 gatgtgttca gcccataagc ttttgccatt gtcggatcga atgatgtgat cttcaattct
    18841 ttataaaaga aaaagacaaa tatcaagacg attccactaa cgagtgctgt cagcaacaaa
    18901 tcgctttccc taacagctaa gacattccca aataaaatat gatacaaatc tgtcgcactc
    18961 tgggcatatg aaatcaagat aatccctaat gcaaaaaagg agctaaagac aattccaatc
    19021 gctgtatcat ttttcaatcg gctccgctga gtcacgaaac caatcactgc tgctgttagc
    19081 atcccaaaag cagtggctcc aaagatgtaa ctaaaaccaa acatataaga aatggctaca
    19141 cctggaagaa ctgcgtgaga gatagcgtct cccatcaaag acatccctcg taaaataata
    19201 aaagaaccga tgactccaga agcaagtcct acaagcatgg aagtgaccaa agcgtattgc
    19261 aggaattgat aattcattaa tccatcaata aaggattgga tcattttttc tctcctcctt
    19321 ttactaaatg tctgatgatc tcaccatagg ccaattgaag gttcttcgat gtaaagactt
    19381 cttgtactgg accggaagcg atcagctgtt tatttaaaag aataacctca tcgaaatatt
    19441 cttctacttt atgaagatca tgatggacga tcaaaatcgt ttttcctgaa ttcttcaatt
    19501 cctgcaagat gtcaaagatc tttcgttcac ttaacgcatc aatccctacg aatggttcat
    19561 ctaaaaagat ccattctgct ccttgggcta gagctcttgc aataaaaact ctctgaagct
    19621 gtccacccga tagttcgctg atctgtcttt tggcatattc ttccatccct acttttttca
    19681 aagcttgtct tgcacgttct ttttcttttt tcccaggtct ttgtccaatt cgtaaagatg
    19741 gatatgttcc taaaagtaca acgcctaata catcaattgg aaaggaaaga tccaattcac
    19801 ttcgctgttc cacgtaggct atttttttac ggactgagtt taccgactgt ccagaaaagg
    19861 tgactttacc agtagtagct ggtatcaact ctagcaaaga tttcataaat gttgattttc
    19921 ctgcaccgtt tggtccgata ataccggtaa ttttttgtgg atttatcgtc aagttgatag
    19981 ttttcaaaac agttttccct tgataagcgg ctgaaaggtc ttgaattaca atggccgtac
    20041 gttccaaatc ttacactcct ttttaggttt acctaacttt ttatttaaat atagtttaac
    20101 taaaatggaa agcattttca agtagaatga tgaaaaagtt tttttcgaat tttcctcgca
    20161 ttttatgaaa attttctgta gaatcacatc aagagttcaa taaggctaac ctaataatga
    20221 aaaacatgtg ttgaaacgtt tgattatttt ttcttgttga ctcaacaacg aaaggagttt
    20281 ttgtatggaa gaagaaaagt taactcgaag tcttagtgcc cgtcatatcc aaatgatcgc
    20341 actaggtgga acgatcggtg tcggtctgtt tatgggtgcc tcctcaacca tacgttggac
    20401 agggccctca gtgatgggag cctatgcgat tgcaggattc tttttgtatt tgattatgcg
    20461 tgctttaggc gaaatgcttt acgttgatcc ttcgactggt tcttttgcaa attacgcgag
    20521 cgagtacatc catcctgtgg ctggctactt gaccgcttgg agtaatattt ttcaatatat
    20581 tgtcgtagga ataagtgaag tgattgctgt aggatcttat atgaattatt ggtggcctga
    20641 tttgccggca attattcccg gaatcattgt cgtcttgttt cttatgctgg caaatcttat
    20701 ttctgtcaag gcgtttggcg aattagaatt ttggttttcg atcatcaaag tcataacgat
    20761 cattttgatg atcattgcag gtcttggggt aatcttattt ggatttggta atcatggtca
    20821 ggcagtaggg atttcaaatc tttggaaaaa tggtggattc ttcactggag gcgtcaaagg
    20881 atttttcttt gcattatcga tcgttgttgc ttcctatcaa ggaatcgaac tgatcggaat
    20941 gactgctggt gaagcagaga atcctaaaaa gacgatcatt gaagctgtcc agtccactat
    21001 cggtcgaatc ttgatttttt atattggtgc tatttttatc attgtgagta tctatccgtg
    21061 gaatcaattg agtgagattg gttcaccttt tgtacaaact ttttctaaag tcggcattac
    21121 atttgctgca ggactgatta attttgtcgt gattacagcg gcattatcag gttgcaattc
    21181 agggattttc agcgcaagtc gtatggtcta tacacttgcc ataaatggaa agatgtctaa
    21241 aaaatttctg aaactatctc gtcacggtgc gcctttttat cctgtcgtag caatctcagg
    21301 tggaatctta attggtctga tcttaaatta tcttctacca atgttcatca accagtcaga
    21361 aaatattttc gttttcgtat acagctcaag tattttgcct ggtatggttc cttgggtagt
    21421 gattttgatc agcgagattc gcttcaaaaa gaagcacgca gataaaatgc atgatcatcc
    21481 ttttaaaatg ccactatctc cttatacgaa ttatgtgaca cttgcttttt tagctatcgt
    21541 attgattttc atgtttatca atcctgaaac gagaatctca ttgattattg gactcgtttt
    21601 cttgctttac atgacgatcc attactttgt acgcgaaaaa agaaccggaa aataaaaaaa
    21661 gaacgaagct tcaccttagt ggaagcttcg ttctttttta ttgcttttat caattcaatt
    21721 ttagcgtctc accgggagtc aggaaataat tctccatgct catcccattc agcgacaaga
    21781 tcgtttctac tggtacgcca gttcgttcgg ctatttgatt tggaccttct ccttccaaaa
    21841 ctacggttgt cgcagcctgt gaagtcgcgc tgttggtatt agtttcagag gaatctgttg
    21901 tgtcttgtgt tgttcgagct tgcatttcag aagcttctac cgactctgct gtctcagaat
    21961 cagaagatgt tgtgtcttgt gtgcttgttg actctgcttg aagagaggcg ctagtctgca
    22021 aataaatatc ggaaatctta gcgacatgat tcgtataatt agccgataat tgattgatca
    22081 aagtatctaa gtctacaccg tctttatttt ctgctgcctg atacgctgca cgtaattttc
    22141 tgatcccttc gtcggagatt gcttgaagct cacgtgcttt ttggttagca actgctgata
    22201 aaccagctat tccctcttga tttccctgaa tttctgtctg gtattcttca atcaatgcag
    22261 gcgtcttttc ttcgatcttt ttcgaataat catttacgat tgcttgaagt tgctctggca
    22321 aaccatttgc tgttttttct tcagaagttt gtgtctcttt tgttttagtt gatgactcac
    22381 ttgtttgttt cgtctctgtt ttctttgtcg ttgtttccgt tatggtcgta cttttcgctt
    22441 ctgacgaatt ttttccatga aaatatagac ccgcaactcc tcctgctatg atcaaaaaca
    22501 cggcaactgc tgccccaatc atgatcatcc gcttacgagc tgcctgtttt tgctgtcttc
    22561 ttcgggtcat ccgaacattt tctccccact cattttcatc atacattttt ttcaccacca
    22621 atttttatgt ataatagcgt tatcaaacgc ttcatattct tattgtagca aataattgtc
    22681 gctgtgcaca tttttatctt tgttatttat aagaaaattt caccagttga gatgattcca
    22741 agattgtccg tcgatttctc cttgcagctg cattgttcgt tcgtctatct cagccatcag
    22801 tttctttatt tcatataaga gcgcacggtc ttgatattct gatttttcgc ttaattcatc
    22861 taattgttgg atcagccaat catttgtcat aatgaagttc ctttcatttc ttgcagtgta
    22921 aaggtcaagt cttcgatctg ctcttttgcc tcagtcagtg cttctacgat atatgcttgt
    22981 ttttcagatt cttcttcctg cattttctga ttttcctgta ctctttgaat aaaccagtca
    23041 atccgctgag tcagcgatgg tttgccttcc aatgagagat actgctgata agcaattagc
    23101 ttctcccaac gtttttcctg ggataaatac ataaattgtg ctaaaacgaa tggcggaaga
    23161 taggtcatat ggaatttatt tgcgattcgc tgatacggcc tcaaaaattc ggaaatcgta
    23221 tattcttctt ttcctccagc ttggtattct ttttccgcca cacctgttgt caatacgatt
    23281 cctagttctt tcccttttaa tacatgtgct gctcgttcca agacattatc ttgccaaatt
    23341 tttaaatgag caggagaact ataccagtaa aacggaaact ggaaaatgat ccgatcatgc
    23401 tgggccaaga gctgtagttc agtctctata tctatttttc cgtctggata acttgattcc
    23461 aaatgatgcc aagtagctgg taaaccagca gaagcttctt tgaaaaattg ctgatttaaa
    23521 gattcttcga tcgttggatg gctaatgatg ataagtgtac gcatcccttt tcctcctttt
    23581 tagtcttcgc tttacagtgt agcataaccg ccacaagttt cttttgccga tgcttgtcat
    23641 tttaaccaga ctaatcaggt tgtccaagaa actgcggttt gtttttccaa ctgttttttc
    23701 aaggtatcca aagcaatatt cttaaaattt tcgtttttca tacgtttacg atagttcgta
    23761 gccgtttctc cggtactctt tttgaacatt cggctaaata cgactagact attgaaccct
    23821 acttgatagg caatactttc cacggattca tccgtcagag aaagaagaaa cgcccctcga
    23881 ttgatccgta ccccgatcaa atattccttg atactggtac ccatatgatt tttgaatact
    23941 cgcgacaaat ggctccgatt gaccgctaaa tgctgggcga tttcctcaac agacaatttt
    24001 tcagcataat gtgcctcgat gtatttgact gtttcaacag ctaaagagga atgaaccttt
    24061 tgtacaccac cgctgacctt tccgccatct tttaaaagga ctgataaaaa acggtaggtt
    24121 aatgctgtta actgtaattc attttccaag gtttcttccg tatctttcat gcattctaaa
    24181 ataatgtcga tatatttttg tatgtcagaa gagaccatcg tgtattggtc ttctctgaaa
    24241 agcgactgcc gaatgatttc ttctgcagca ctcccaccaa atgaaagcca gcaatacatc
    24301 cacggatctt ctccatctga taagtaaaac gtcgagtctc ctggacgaat caagaacaga
    24361 tcaccttttt tcaattggaa ttgttgattt ttcacatgat aagatccttt gccttccatc
    24421 acgacatgca ggatgtaatc attgcgaata gcaggaccaa aagaatgaaa cggcatcgtt
    24481 ttagagaaac cacaaaaatc aaaatacaat tctttttcta cttgttgatg ttcctttttt
    24541 aaaaaaatcg attgctccac gtctatccct ccttactcct ttagtataat gcaacaaaaa
    24601 gttaaataaa agtcacagaa atatattaaa aaggaagcga tttcttgtta agtttaagtc
    24661 attaaaagat ctttgttatt cgttgtaacc ggtttactta gtattatata ttgaacagga
    24721 gaggaataca gattatgaaa agcaaaatga tccaacgtgt gtcctacgct ttaggcgctt
    24781 ttggacacga cacattctat gttacattaa gcacttattt tatggtattc gtgacaagcc
    24841 agatgttcgc aggaacagat aaggcaacga atgcaaaaat gattgctaca gttacaagct
    24901 tagttgtagg tatccgctta gttgaaatta tttttgatcc tttgattggt ggcatcatcg
    24961 ataatactcg tacaaaatac ggtaaattca aaccatggtt agttatcggc ggcgttgtta
    25021 gttccgtact cttggtagca atctttacca atttctttgg cttagctact cataatacga
    25081 ctttatttat catattattt gcaatcgttt tcatcttatt ggatagtttt tattcattta
    25141 aggatatcgc tttttggtca atgattccag ctttgagtac ggaaacaaag gaacgtgaaa
    25201 aatttgctac gtttgctcgt ttcggctctt ctctaggtgc aaatggtact actctagtcg
    25261 ttgtccctat cgttactttc tttacctatc ttgttactca taaacatatt gaaggcgctt
    25321 caggctggtt ctggttcggt gtgattgctg ctgttatttc aggcggtact gcgattatca
    25381 ctgccttggg cacaaaagaa gtcgatactg cgatccgtca gcaaaatgaa aaagctggga
    25441 tcaaagatgt gttcaaagca gttgctcaaa atgatcagtt gatgtggctt gcccttgctt
    25501 atatcgctta tgcgattgcc aatgtagcaa caactgccgt attattctac ttcttcaaat
    25561 atgtacttgg ccaaccaact gaattttgga tcgttggtct agttgctgcc ttacttggtt
    25621 tagtagctgt tccactattc ccaatgctta cacgcgtcat ctctcgtcgc tacgtttacc
    25681 ttggagggat cgcaatgatg atttcttctt actttttctt tacgatcgca ggttcaagtt
    25741 tgccaatcgt tatcatcggg ctagtattgt tctacttccc tcaacaattg attttcttat
    25801 ccgctttgat gacgatcaca gactctgttg aatatggtca atggaaaaat ggtatccgta
    25861 acgaagctgt tactctatca attcgccctt tactagataa aatcgctggt gctttgtcta
    25921 acgggatcgt agggtttgtc gctatcgctt ctgggatgac tggtaatgct actgctgctg
    25981 atatcactgc tcatggcgta acgactttca aagcttatgc attctatgct cctgctgctt
    26041 tgatgatttt ctccgctatt atttttgctt ggaagattac attgacagag aaaaaacatg
    26101 cacaaatcgt tgagaaattg gaaaacaaat tggctccagc tgatacaatg gaagatgaac
    26161 ttcacgatgc agtagttcct tcccattaag aaaggatctt gaaaatgaag attacagaaa
    26221 aagattttgg ccaaggttat cacttgataa ccttggagaa caaaaacaag ctagcacttt
    26281 ctatctctga tttaggtgct agaatcgtca gtttaaaatc aaatgatcga gaattagttt
    26341 tgggatttga caccgctgaa gaatatatcg aaaaagatcc gtatatcggt gcttctatcg
    26401 gtcgcaccgc agggagaatc gaaaatggcc gcttttctct caatggaaaa acttaccaat
    26461 tagccacaga tccaaaaact ggacataatc tgcatggtgg tgctcctgga tttgaattaa
    26521 aaaaatggtc ttatgtcatt ttgaatggcg aaaatgaagc ttctgtcatt tttactacta
    26581 caagtcccga cggagaacat ggttttccag gtacaatgga tgtggaaatc cgctatactt
    26641 tgacaaaaga taatatctgg cgagtgacct ctcgcggcac cagtgaccaa gatactttat
    26701 tcaatccgac aaatcatgtt tacttcaact tgacgggaga tgcttctcag tcaatcgatc
    26761 aacatgagct ttggctcaat agcgaagctt atgctccttt gcgaacagat agtattccta
    26821 taggggtaaa agaaaatgct gcaggaagtg ctttcgattt tcaaatacct aaaaaattag
    26881 cgtccgtttt tgctagtgat ttggatcaaa aaaatcttgt tgacggaatc gatcatccat
    26941 ttttcttaaa agaaaccggc ttgggtaaag aagccgctcg cttgactagc cccgataaaa
    27001 gaatccaagt agatattgct acagatgctt ctagtgtggt tattttcact gcaaatttcg
    27061 gcacagaaac accagaaatg cgcaaccgaa aactggcaca tcacggtggc atcacatttg
    27121 agactcaaac agctccaggt gcagagcgct tctcagcatt tggttcgatt catttaaaag
    27181 ccggatcagt atttgaaaca gtaacagaat tcaaaatcaa aacaagaaag gaataactca
    27241 tcatggaaat caaaagaagc ttaaacacaa aatttgccga aacattcgga cctaaaaaaa
    27301 ctacacaata cttctcacca ggacgcatca atttgattgg tgaacacaca gattacaatg
    27361 gaggccatgt cttccccgct tccatcactt atggaacgca aggtgtagca gcacctcgag
    27421 acgataataa aatattagtt tattccacaa actttgacga tatcggtgtc attagtttta
    27481 ctttagatga gctagaatat gataaaaaag caaactgggc aaattacgta aaaggaatgg
    27541 tccttaaatt aaaagaagct ggacactcaa tcgatcatgg gtttgagtta ttagtggaag
    27601 gaacaatccc taatggtgca ggactttcca gtagcgcttc attagaatta ttagtaggtg
    27661 ttgtattaga agatctgttt gatttaaaaa cagatcgctt ggaactagtc caaacaggta
    27721 aaaaagtaga aaatgaattc atcggtgtca attcaggtat catggatcag tttgccatcg
    27781 gcttcggtga gctagatcat gcaatcttat tagatacaaa tacgctgaaa tacgaaatgg
    27841 ttccagtgaa actagatggt tatgcaatcg tcatcatgaa tacgaataag cggcgggaat
    27901 tggcagattc aaaatacaat gaacgccgta gcgaatgcga agaagccttg gcacgtttgc
    27961 aaaaaacttt gcctatctct tctttaggag acttagatga agaagaattt gaatcaacta
    28021 ttgaccaaat cggcgatgat acattgatac gcagagctcg ccatgccgtt tctgaaaatc
    28081 agcggacttt gaaggcaaaa gctgaattag aagctgggaa cttagaagca tttggtcaac
    28141 tgttaaatga ttctcaccac tctcttcgct atgattatga agtaactggg atcgaattag
    28201 atacactagt agatgctgct caaaaacaag aaggtgtctt aggtgcacga atgacaggag
    28261 ctggatttgg tggctgtgcg attgctcttg tcaaagaaga aaatatacct gccttcaaaa
    28321 acaatgtcta tgatgaatac atgaaagttg tcggctatgc tccggaattt tatgttgcac
    28381 acattggaaa tgggacaaca aagatcgatc cagaagctgt tcatgtttga taaaaaaatc
    28441 aacgatctca gctaaaaaca aatagataaa aataacctct cagcaatttc caaatgtgag
    28501 ccttttgctc gtatttgaaa attgctgttt tttattgaca cggaaagaga acgtgtgttc
    28561 taattgtgtt gaagaggtga ttgttatgtg ggcgaatcaa aatccggtat ttgtttatga
    28621 aaaagaacct tcaagagata ttttatgtat tgactgcaaa tctttttacg cctccgtaga
    28681 gtgcgttgaa aaggatcttg atccccttac aactaaatta gtagtcatgt cctatccgtc
    28741 cgattcgaca gaaacacggg gtagcggatt aatcttggct tccagtcctg cagcaaaaaa
    28801 agcttacggc atcaccaata tcagccgtgc cagagatctt cccttcccct atccaagtga
    28861 tttatatatt gttgccccaa ggatggccta ttatatggaa aagaacagag aaatcaattc
    28921 tatctataaa aaatatgcag acgaagcgaa ccatcatgtc tattccgtcg atgaaagctt
    28981 ccttgatgta acaaatagca tggcactctt tggtaaaaca aatgcgtacg acatggctaa
    29041 actgatccat aaagatgtgt ttgagcagac cggaatctac acgactatag gaattggcga
    29101 taacccgcta cttgctaaac tagccttaga caatggctca aaagacggaa agcaaatgtt
    29161 tgctgaatgg cgttatgaag atgtaccaga aaagttatgg aaaatctcag atatcactga
    29221 cttttgggga atcggcaaac gaacagcaaa acgtctgaaa caaatgggta tacattctgt
    29281 ttatgattta gctcacgcgg attattatca cttgaaaagc aagctagggg tcatcggtac
    29341 tcagctttat gcccatgctt ggggcatcga tcgaagtttt ttgggacaag aatatacacc
    29401 aaaatcaaaa agtatcggaa atagtcaaat cctagataga gattatacaa gacgagaaga
    29461 aatcgagatc gttatcaaag aaatggccga ccaagtcggc actcgcttga gacgatctgc
    29521 agcaaaaaca caagtagtca gcttatgggt aggatactct ttaggctatc ttgatccaaa
    29581 tggcaagacc gggtttcacc agcaaatgaa aatcgaagcg acaaatgcta gcagagatct
    29641 agcagatgct ttattgacca ttttccacca gcattaccac taccaggata ttcggcgtgt
    29701 aggcgtgaac tgttccgacc tcgtatacgc gacaggttta cagcttaacc tttttgaaga
    29761 tcctgaaaag cagataaatg aagcgaaagt tgattttgtt gtcgatacga ttagaaaaaa
    29821 atacggcttt caatcaatca tccatgcgca tagtctcttg caaggaggac gcgccatcgc
    29881 acgtagttct ctcgttggcg ggcatgcagg tggtatgagt ggattagaag gtggaaaaga
    29941 tgagacggac agcaaaaaaa ttcattgact ataacgagta tcgagatcgg ccatttggat
    30001 taaaatgggg gactgctttt gcgatggaag aactagtcaa cggtatcaaa aaaatgaaga
    30061 agaagcacgc aaacagcctc ttcctaaaca acaaatgaca ttgcaagaaa tcgatagtat
    30121 actgacagaa gcctttttat ctcgtaaagc agtaatcatt cagctcaatt taaaagatga
    30181 atttggaaga ctcttggatg aggtcgaagg gcacttcatt ggtgaagcct atgaagatta
    30241 ctttatgatt gaccatcagc cgatattgtg ggaagacgta cggtatattg agttaaagga
    30301 aacaaaaaaa tggtttgtga catgagaggt tgtgacagaa gtgatcaact tcaaaaaata
    30361 agaaaggatt tttcgaaaat tgcttcttaa atttttgtgg aattttggct tatttttcga
    30421 agaagcaact tctggaacaa cgtatattag atatcagagg ttgtgaaaaa gtcgcttagt
    30481 tttgagtaac aagccaaggc gctgatttca caacctctgt ctatctatcc atctattcgg
    30541 taagattcat gcatgacgtc ttctttattt agtatgtaga gccctttttc ttctgaccat
    30601 tcaacataaa tcagctgttt tacatctgca tagccttctt tcttcaagtt ctccataagc
    30661 catgcttcag tcaattgatt ggactgaagc actttatgct gaatttgccc ttcatctaca
    30721 agcaggatcg ataaagcttt gtcttcatct gattttttca atacactcat ttgaccggca
    30781 ttctctagga taacatgttt ggcattttca agaaaaaagc attcttgctg tcttaagatt
    30841 gctcgcaact gctccatctc gatatggtta tttgtcaact ctgtcatgtt gatgacgccg
    30901 tctttgatta gaacagatgg ctcgcctttg acccaacgat tgaccttttc atttttttgg
    30961 acgatacgtt cgataccata aatcattact gcccaaagcg caatggcaaa taggacatgc
    31021 cctacatgga cattgtcatc ataggttgat tcttctaaga tcccacctaa aacaagcgta
    31081 tagactaaat caaacggtgt gatttctgac atggatttct tcccaagcaa acgtaccact
    31141 aataataaac cgatcaaccc gaacaccagt ttcattgtaa ttgaaaaaat cattttttct
    31201 ccccctaact atctgttaat taaattatat atgctttctc acgaacacgc atggaatata
    31261 caaggaagaa atgtatcctt ttactaaaaa aaggactgtg acactgtagt gacccccaaa
    31321 agttagactt ttttggagtg gagaaatcca ctcctttttt ttatgctcta aaatgatatt
    31381 tttacacaac cgttacggtt gttttacgaa attggacgag actttgccag tttaatttct
    31441 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
    31501 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
    31561 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
    31621 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
    31681 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
    31741 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
    31801 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
    31861 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
    31921 gatggat
//
LOCUS       DAZUKC010000029        30202 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000029 DAZUKC010000000
VERSION     DAZUKC010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.030"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(61..2424)
                     /locus_tag="ACG80T_001248"
     CDS             complement(61..2424)
                     /locus_tag="ACG80T_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HFS4840910.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            2589..3093
                     /locus_tag="ACG80T_001249"
                     /pseudo
     CDS             2589..3093
                     /locus_tag="ACG80T_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3201..3548)
                     /gene="rbfA"
                     /locus_tag="ACG80T_001250"
     CDS             complement(3201..3548)
                     /gene="rbfA"
                     /locus_tag="ACG80T_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFS4840911.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(3575..5881)
                     /gene="infB"
                     /locus_tag="ACG80T_001251"
     CDS             complement(3575..5881)
                     /gene="infB"
                     /locus_tag="ACG80T_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFS4840912.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(5894..6205)
                     /locus_tag="ACG80T_001252"
     CDS             complement(5894..6205)
                     /locus_tag="ACG80T_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFS4840913.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(6202..6495)
                     /locus_tag="ACG80T_001253"
     CDS             complement(6202..6495)
                     /locus_tag="ACG80T_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFS4840914.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(6517..7692)
                     /gene="nusA"
                     /locus_tag="ACG80T_001254"
     CDS             complement(6517..7692)
                     /gene="nusA"
                     /locus_tag="ACG80T_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFS4840915.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(7716..8189)
                     /gene="rimP"
                     /locus_tag="ACG80T_001255"
     CDS             complement(7716..8189)
                     /gene="rimP"
                     /locus_tag="ACG80T_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFS4840916.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(8333..12685)
                     /locus_tag="ACG80T_001256"
     CDS             complement(8333..12685)
                     /locus_tag="ACG80T_001256"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFS4840917.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            complement(12897..14606)
                     /locus_tag="ACG80T_001257"
     CDS             complement(12897..14606)
                     /locus_tag="ACG80T_001257"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFS4840918.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            complement(14674..15942)
                     /gene="rseP"
                     /locus_tag="ACG80T_001258"
     CDS             complement(14674..15942)
                     /gene="rseP"
                     /locus_tag="ACG80T_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFS4840919.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(16103..16903)
                     /locus_tag="ACG80T_001259"
     CDS             complement(16103..16903)
                     /locus_tag="ACG80T_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFS4840920.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(16900..17712)
                     /locus_tag="ACG80T_001260"
     CDS             complement(16900..17712)
                     /locus_tag="ACG80T_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFS4840921.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(17907..18464)
                     /gene="frr"
                     /locus_tag="ACG80T_001261"
     CDS             complement(17907..18464)
                     /gene="frr"
                     /locus_tag="ACG80T_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFS4840922.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(18467..19189)
                     /gene="pyrH"
                     /locus_tag="ACG80T_001262"
     CDS             complement(18467..19189)
                     /gene="pyrH"
                     /locus_tag="ACG80T_001262"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFS4840923.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(19325..20206)
                     /gene="tsf"
                     /locus_tag="ACG80T_001263"
     CDS             complement(19325..20206)
                     /gene="tsf"
                     /locus_tag="ACG80T_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFS4840924.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(20305..21087)
                     /gene="rpsB"
                     /locus_tag="ACG80T_001264"
     CDS             complement(20305..21087)
                     /gene="rpsB"
                     /locus_tag="ACG80T_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFS4840925.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            21446..21925
                     /locus_tag="ACG80T_001265"
     CDS             21446..21925
                     /locus_tag="ACG80T_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFS4840926.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            22142..23233
                     /gene="phnW"
                     /locus_tag="ACG80T_001266"
     CDS             22142..23233
                     /gene="phnW"
                     /locus_tag="ACG80T_001266"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFS4840927.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            23226..24011
                     /gene="phnX"
                     /locus_tag="ACG80T_001267"
     CDS             23226..24011
                     /gene="phnX"
                     /locus_tag="ACG80T_001267"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050194 - phosphonoacetaldehyde hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFS4840928.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(24357..26048)
                     /gene="argS"
                     /locus_tag="ACG80T_001268"
     CDS             complement(24357..26048)
                     /gene="argS"
                     /locus_tag="ACG80T_001268"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFS4840929.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(26470..27417)
                     /gene="arcC"
                     /locus_tag="ACG80T_001269"
     CDS             complement(26470..27417)
                     /gene="arcC"
                     /locus_tag="ACG80T_001269"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFS4840930.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(27532..28551)
                     /gene="argF"
                     /locus_tag="ACG80T_001270"
     CDS             complement(27532..28551)
                     /gene="argF"
                     /locus_tag="ACG80T_001270"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFS4840931.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(28642..29871)
                     /gene="arcA"
                     /locus_tag="ACG80T_001271"
     CDS             complement(28642..29871)
                     /gene="arcA"
                     /locus_tag="ACG80T_001271"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFS4840932.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         8079 a         6474 c         5359 g        10290 t
ORIGIN      
        1 cttaatttga caaataatac aatgtaaaaa attctgtttt gcgggatcga ttttatcatt
       61 ttattggtca agatttttcc atttccaatc caatacttct ggcagatcaa taccattttc
      121 acgaatatag ttttgatgtt cttgtaattt ctgatccatt ttttccgcaa aatcttctgc
      181 ttgacttcct ttgattttta aagctgcgtc ttttgccaaa tgataacgat caagttcatt
      241 tactacacgc atgtcaaatg gtgtcgtgat atctccattt tctttgtagc catgaatcat
      301 taaatgatgg ttatgacgat caaagaaaat cgtgcggacc aatgtttcat aaccgtggaa
      361 agcaaataag atcggtttgt cttttgtaaa atattgatca aattcttcat cgctcaaccc
      421 acgcggatct tgacttggat gacgtaattt caataaatct acgacattga tgaaccgaat
      481 ttttagtttt gggaattctt ccagcagcaa agttacagct gctaatgctt ctaagttaga
      541 ttccgttcct gcagcagcaa tgaccaattc aggttcctcc tcttttgctg tcgaagccca
      601 atcaatgatt ttcaagcctt cattcactaa gacggctgct tcttctgctg agtaaaattg
      661 agcacgcgga tgtttggaag agatgatcaa attgattttt tctgacgagc ggaatgcttt
      721 atccatgact gcgagcaatg tatttgcatc cgcaggcaga tattcacgga tgaactccgc
      781 cttcttttct gccaaatgtg tcaatattcc tggatcttgg tggctatatc cattgtgatc
      841 ttgctggaag actgttgagc ttgcaatgag attcagcgaa ggataatctt ttcgccaaga
      901 aaggtcatgt gatttccgta tccatttgaa gtgctgagtc aacatagagt caaccactcg
      961 caagaatgat tcgtaacttg caaaaaaacc atgccgtcca gtcagaacgt acccttctaa
     1021 gaacccttca tcttggtgtt ccgataattg actgtcgatg acgcgaccag atggcgaaag
     1081 ccattcatca ttttctggtt cgattttttc catccattgg cgatttgtcg ttttgaaaac
     1141 ttgattcaat cggtttgatt ttgtttcatc tggtccaaat atacggaaat tttctggatt
     1201 tttctcaatg atctcgcgaa cgaatttacc aagttcggtc atatcttctg ctttaacgga
     1261 gcctggtttt tcaaattgca aagtatattt tttccaatct ggcataataa gcggtcttgg
     1321 atcgattcca ccattggtga taggattttt tgccattctt tggtcaccag taggcgcaat
     1381 ttctctgatt tcttctaaaa tacgtccttg tgcatcaaat aatttttctg gttcatagga
     1441 tttcagccaa cggagtaaag catctgcatg atccatatgt tcttgatcta caggaatcgg
     1501 tacttggtgt gctcggaagg ttccttcaat tggttccccg tcccattctt ttggaccggt
     1561 ccatcctttt ggtgtgcgta cgatcaatac tggccatttt ggcatcacga catctgttgc
     1621 tgtcttcagg cgagcttctt tttggatgga tttgattttc tcgattgctt gatccatttt
     1681 ttcagccata acaggattta atttttctgg atcatttcct tcaataaaga aaggctccca
     1741 gccaagtccg ttaaagtaat ttgctaattc tccatctgat ttacgtgcca agatcgttgg
     1801 gttggctatt tttgcaccat ttaaatgaag gatcggtaaa acagctccat ctatcactgg
     1861 attgataaag acatttgaga accagcttcc agctaaagga ccggtttctg cttcaccatc
     1921 accaatcacg acagcagaga ttacttcagg attgtctaat acagctccga ctgcatgtga
     1981 aagagagtat ccaagctctc cgccttcatg gatagaacct ggtgtttctg gtgctgcatg
     2041 agaagcaatg cctccaggaa atgaaaatcg cttgaataat ttttgcattc ctgtctcatc
     2101 ttccgttact tctggataga tctctgtgta acttccatct aaataagcat tcgataccat
     2161 gacttgtccg ccgtgtcctg gcccttcgat ataaaacatg ttcaaatcat atttattgat
     2221 gactcggttc aaatggacat aaataaaatt ttggccagga atcgttcccc aatgaccgat
     2281 tggatgtttt ttgacatctt ctggtttcag tgtacgtctc agtaaggggt tatctttaag
     2341 ataaagttgc ccaacagaaa gataattagc agcacgccac caagccgtca ttttatcaaa
     2401 gtattcttta ctagaataat ccatacataa cactcctttt cattattact cttattctag
     2461 cgcagatact cccaattgaa acctaataag cacttctgtt catttgtttg gattttatat
     2521 ctagccaaat gaacaggtat aaaaaaccac gaaatttttc ctttcgctga cgttttttac
     2581 attgatccaa aaaacttgtc gttatcacaa tggctgttcc gcagttcatt tcgctcctca
     2641 tcatgcataa tttgctgaat gacgctaggc cagttaacac attgttggaa aactggggct
     2701 ggatcgagca attcctttcc tgattgatcc gttttatgta aaagtctctg ttatctttgt
     2761 caatatgtga attggtatcc cagctactat gctaatttct acagggatta tccaaaattt
     2821 ggatcgtgac caaatcgaag cggcgtaatc ggaaatatca ataacttcaa cgtgattttt
     2881 cttttaactg tcaacggacc gacaaactca aacttttatg gtgcaggttc cactgatctt
     2941 ctagttactt ggctatacct gactgtcact aaaatagatt acaatctagc ttccgttatc
     3001 ggtatcttga tctttatcta tatgcaaaaa tttatatact atagaaaaaa gaaagtcgaa
     3061 ttttttactt ttctttttct cttttttggt tagctgacac gctaccagac taaagtcatg
     3121 gaactcttag gtaatacgtc ttaatgaaaa caaaaaaatc ccgcaacccc tgatttcaca
     3181 ggatttgcgg gattttttga ttagtctttg ttcaagtcgc gaagcaattc atcgatatgg
     3241 ttcccataga caactgattc atcaagttca aagaacaatt caggtgtctt ataaatactt
     3301 agattgtgcc ctaactctcg acgaatcaat cctgatgctt tattcaatcc atcttgtgct
     3361 tttttcttat ctgatgctaa gtcagataaa atgctgtaat agatcgttgc ttgctgtaag
     3421 tcccccgtaa ctttcacatc tgtgatcgtt acgttctcca cacgagggtc acggaccttt
     3481 ttgcgcaaaa tatcattgac ttcttttaag atttcttgac cgactcggcg atcacggtag
     3541 ttagccataa tgattggcct ccttttatca gtttttagtc atttttgact tcttccatga
     3601 taaatccttc aattacgtca tctactttga tgtcattgaa tttttcaatc atcgcaccac
     3661 attcataacc cattttgact tctttcacgt catctttgaa tcgtttcaga cttgcaagtt
     3721 ggccttcata gattacgatt ccgtcacgga ttacacggac accgctatct ctacgaatat
     3781 atccttctgt tacataggca cctgcaatcg taccaacttt agaaactttg aaggtttcac
     3841 gaacgatcat ttgaccggtg attttttctt caaattcagg gtctagcatc cctttcatcg
     3901 cagtttcgat ttcttcgatg gctttataga tgatacggtg aagacgaata tcgacttctt
     3961 cagtatcagc ttgcactttt gcctgcggtg taggacgaac gttgaaaccg atgataatag
     4021 cgttgctggc tgctgctaag gttacatcac tttcattgat tgctccgaca gctgaatgga
     4081 cgatttttac acggacacct tccacatcaa tcttcttcaa tgatgcagcc agtgcttctg
     4141 ctgaaccttg tacatctgct ttgatgatta cgttaacttc tttcaattcg ccttctttca
     4201 ggctttcaaa taagttgtcc aaagtcacac ggcttgtggc agcacggttt tccaataatg
     4261 cacgttttgc gcgttcttca cccgctgcac gtgctgtctt ttcgtcttcg aagactacga
     4321 aacggtcacc tgcttgaggt acatcattca agccagtgat ttctactggc gttgcaggtc
     4381 ctgcttcttt atcgcgacgg ccaagatcgt ttgtcattac acggacacgt ccgtaagtat
     4441 taccgacaac gattgggtca ccaacgtgaa gtgttccttc ttggacaagt aaggttgaca
     4501 caggaccttt acctttatca agacgtgctt cgatcactgt tccgattgcg cgttgtgttg
     4561 gatctgcttt taaatcttct acttctgcga ctaacaggat catttccagc aattcttcga
     4621 tgttttggcc gaatttcgct gagatctcaa caaaaatcgt ttctccaccc catgcttcag
     4681 gaattaattc atactcactt aattcttgca taacatgctg aggatttgca cctggtttat
     4741 caattttatt tacagcgacg atgattggaa cgcctgcagc tttggcatgg ttgattgctt
     4801 caactgtttg tggcatgacg ccatcatctg cagcaacaac taagatcgtg atatctgtga
     4861 tacttgctcc acgtgcacgc atacttgtaa aggccgcatg tcctggtgta tccaagaaag
     4921 tgattggttt accatcaata tcgatctggt aggcaccgat atgctgtgtg attccgccgg
     4981 cttcgccgct tgttacacgt gaatggcgca atgtatccaa taaggttgtt ttcccgtggt
     5041 ctacgtgtcc cataatcgtt actactggcg gacgtgagac taatttatct ggatcgactt
     5101 cttctgcctc gaagaattta tcgatatcag caatgtcaac ttggactttt tcttgtggtt
     5161 ccatgccata gtccgtcgct aacagttcaa tcgtatcttt atccagtgct tggttttgat
     5221 tgaccatgac accaagcata aataattttt taatgatttc tgctggctca cgatgaattt
     5281 ttttcgcaat atctgctacg ttcatacctt cagtatactc taatacatct ggcaattcac
     5341 ggaatttgcg tggtggtaca gctggtttag ttgattgttg ctgtttgcct tttttacctt
     5401 ttttattata acgattacgg ttattattat taaatttgcc gcgattgtta ttgtttggtt
     5461 tgttgttatt gttagtacgg ttttgattgc cgccttggct cgttccttga cgattttggc
     5521 tgctggtatt tcccgtatta ttttggtttg ttgttggttt ttttccttga ttcacttgac
     5581 cgctcccttg accgcgatct tgataactgc ggttattttt atttttttgt tgattgttcg
     5641 ttgtttcttt tttacgttga tcgttcggct gttgattggc tggttttctg cttgcttgtt
     5701 gtgcagcttg ttgtttcgtg cttgggtttt tgaacgcttg ttgcaatttc ttctcatcac
     5761 tgctagtaat cgcacccata tggtttttca catcgatgcc aagttgttgt gctttttcca
     5821 cgacgtcttt actagatttt ttgagttctt ttgctaattc atagattctt ttgttgccca
     5881 tgcaatcacc ttcctaacct tcaattagct ccttggtctt cttcgcaaat ccggcatcca
     5941 ttattccgaa taccttgcga ggttttccga tcatctgcgt gatttctgct tcagaaaata
     6001 gctcaaagca aggaacttca taatatgaac tcttatcttt tatctttttt cttgtattct
     6061 cacttgcatc ggttgccaca aacaccaatt ttgctttttg tttgcggatt tcagcaatgg
     6121 tcagttcttc acccgtgatt agttttcccg cgcgcatcgc tagaccgatg agattcatgg
     6181 ctttttgccg attaactgta ttcattttcc aaataactct ttacgtgctt tttggtgagt
     6241 gacatagtca agaagttctt gataaaactc atctgttaat gttgtttcga gtactcgatc
     6301 gagaattttt ttatcccaag cttgttgtac ttcttccggc tcaagtgaga catacgctcc
     6361 tcgtccaggc atttttccag ttggatcgat cgatacttcc ccttcttttg aacgggtgat
     6421 tcggaccatc tcttttttag gtttcatttc acctgaaacc acggatttgc gtaaaggaat
     6481 tttcctttgt ttcatctctt tgcctcctta ctaaagttaa gcttctggtt tttcttcgac
     6541 cgtttcattg ttgaatgcaa ttgtttcata ttcatcgtct gttaaatctg actgaacgat
     6601 tgcttcatca tgcagttctt ctgtttcttt ttgttgttct tgattttcat aaaattccgt
     6661 catgtcagat tctgatttga tatcgatttt atggttagtc aatttagcag ctaaacgtgc
     6721 attttgtccg cgtttaccga ttgctagcga taattggtag tctggaacga caactgtgca
     6781 ggcctttgga tttgtctcat cgaagatcac atccatgact tgtgcaggat tcaatgcatt
     6841 tgcgatgaat acagctggat cttcattcca ttcgacgata tccatgtttt cgccttttaa
     6901 ttcattgacg attgcttgaa ctcgttgtcc ctttggcccg acacatgtac cgactgcatc
     6961 gatatttgga tcatttgagc gaactgccac ttttgaacga tcgccagctt cacgggcgat
     7021 gctgacaatt tcaacgattc catcatagac ttctggtact tcttgttcaa ataagcgacg
     7081 caataggtct ggatggctgc ggctaacaaa tacttgcgga cctttagacg tattttctac
     7141 tcgggaaaca taaactttga ttcgatcatg tggttgataa aattcatttg gcatttgatc
     7201 ttgtttagaa agaactgcct caattttacc aagattgaca taaatataac gtttatcctg
     7261 gcgttcgacg attccttgca tgatgtcttt ttcataagca ctgaattcgt tataaataat
     7321 cgttctttct gcttcacgta cacgctgtaa aataacttgt ttagccgttt gcgcagcgat
     7381 ccgtccaaaa tcttttggtg ttacttcaaa acggatcgta tcaccgatct catatgctgg
     7441 attgattaaa agtgcatctt tcaatgagac ttccaactgt gaatccatca cttcttccgt
     7501 tacttctttt accgcataga catggatctt tccttttttc tgatcaaatt ccacttcgac
     7561 gttttgtgct tggccatagt gacgtttgta tgcagaaacc aatgctgctt ctaaagcatc
     7621 gatcacgatt tcttttgaga tccctttttc agcttctaaa gcgtctaacg catttaacat
     7681 ttctttactc atttttttct gattcctctc cgtgattaaa attggatagc taagcgagct
     7741 tttgcaatat tttttcggtc aaaagtcaat tctttttctc gtgtttttat gcgtattttc
     7801 aatgtaagct gttcagcatc aaatgattga agaaatcctt catattgttt ttcgccatct
     7861 actggttggt ataaggagac atggacataa tcaccaagtg ccttttcata gtcgctttct
     7921 tttttcaatg gccgctctgc tcccggggag gatacctcca ggaaataagc ttgaggaatc
     7981 ggatctggat ctgctgcatc aagtttttca ctcagtttct cactgacaaa ggcgcattct
     8041 tcgatatcga taccgccttc tttgtcaata aatactcgta agaaccagct ttttccttct
     8101 tttacaaatt cgacttcgac aagttcaaaa ttttgttcat ctaaaatcgg tgtcaccatt
     8161 tctgtgactg tttcaacgac gctactcaaa agtttcgcct ccttaaaaat taagtgcgta
     8221 attccattaa aaagagtgag cgaaaaatcc gctcactcac agttagtatc attacctata
     8281 agaactatac catactctgc ttaaaattac aagcagacag gaaaacagat gcttatagca
     8341 tatcgaataa cgataattga ttttcatctg gtagatcttt caatacgcca ttgtcgttca
     8401 tatattcgat caatgttttt gaaactttac cgcgggtggc aagatcttct ttggataaaa
     8461 atggaccgtc ttttcttgct tcaacgattt gtttcgccac gttggttccc aaacttggga
     8521 cagctctaaa tggtgcgatc aacgtatcac cttcaataac aaagttaacg gcatccgatt
     8581 tatacaaatc aatcattcca aatttgaatc ctcgttccag catttcattg gctaactcca
     8641 gcactgtcag ttgattcttt tcttttacgg aagcatctaa tcccttgtcc gtgatttctt
     8701 tcattgcttg tttcacagca tcttttcctt tgcacatgga gactagatca aagtcgtctg
     8761 cacgtacaga aaagtatgca cagtaataca aaatagggaa ataaaccttg aaataagcga
     8821 cacgaagagc catcaatacg taagctgcag catgggcttt cggaaacata tatttgattt
     8881 ttgaacaaga atcgatatac cagtcgggca cattattttc tttcatcgcc gatagatagg
     8941 tttctcgcaa ttcatcaggg atcttgttcc attgtccttt acgaacagtt tccatgattt
     9001 taaatgccat cccgctatct aatccagcat ggatcaaata aaccataata tcatcacgac
     9061 agccgattac ttccgccagt gtcgcatctc ctcgtcgaat caattcttcc gcatttccta
     9121 accatacgtc tgtaccatga gatagtccgg aaatttgcag tagttctgca aaagttgtag
     9181 gatgcgtttc ttctaacatc cctcgaacaa aacgagtgcc gaattctgga atacctaatg
     9241 tccctgtttt ggaataaatt tgttcctgac ttactccaag tacttcagga ccggcaaata
     9301 tccgcatcac ttccgggtca tccgtaggga tcgtttgtgg gtcgatccct gataaatctt
     9361 gaagcatccg aataacagtt gggtcatcat gccctagaat atctaatttc aatacattat
     9421 cgtgaatcga gtggaaatca aaatgagtcg ttttccattc tgaattttga tcgtctgctg
     9481 gatattggat cggcgtaaaa tcatagacat ccatataatc gggaataacg atgatccccc
     9541 ctggatgctg gccggttgtc cgtttgactc cagtagcgcc ttttgctaat ctgtcgactt
     9601 ctgcacttcg aaactgcaga ttattgtccc tttcgtatcc ttttacatag ccatatgctg
     9661 ttttgtcggc gactgtacca atcgttccgg cacggtaaac atagtcttcg ccaaacaata
     9721 ctttcgtata gttatgggct tctgcttgat aatcacctga gaagttcaaa tcaatatcgg
     9781 gcactttgtc tccatggaat cctaaaaatg tctcaaatgg aatatcatga ccgtctttgt
     9841 ttaagcgtgc accacatttt ggacattgtt tttctggcat gtcaaatcca gaaccatacg
     9901 taccatcttc aaaaaactcg gaatattggc actctggaca ataataatga ggggctaatg
     9961 gattaacttc agtgattcct gtcatcgtcg caacaaagct agagccaaca gagccacgtg
    10021 aacctactag atagccatct tcattacttt tatgcactag tttttgggcg atcagataaa
    10081 taaccgagaa gccattacca ttaatagaat tcaactcttt ttctaatcgt ttttgaatga
    10141 tctcaggtaa cggatctccg tacatttgtt ttgcttttgt gtaactcaat tctgatattt
    10201 catcttctga acctggaatc ttcggtgtat aaagttcatc tttgactgga atcacttctt
    10261 cgcagatatc tgcgatttta ttcgtatttt ctactacgat ctctttcgct gtttcttccc
    10321 ctaaaaagtg aaatgcagtt agcatctcat cggttgttcg gaaatggaca tccggcaaac
    10381 tatgacgatt caacggattc gctccgccca tagaattgat caaaattttt cggtaaatgg
    10441 catcttcttc gttcagataa tgtacattac ctgttgcaac aacgatttta tccaaacttt
    10501 ttccgatttc taccaaattt tggataattt cttccaaatc atgttcattt tttaccaatt
    10561 cttgctcgat caaaggcgca tagaccgctt ttggcataac ttcaatataa tcatagaatt
    10621 ttgctcggtt tcttgcctct tctacccctt tttgcatcat ggcttcgaat atttctccct
    10681 tatcacaagc tgagccgatc aatagatttt cacgcatctt tttcaattgg gagcgtggta
    10741 ttcgtgggac gcgttcgaag tattcgacat tcgacattga aatcagtttg aacaaatctt
    10801 tcagtcctgc ctgatttttt gctaaaatcg ttacatggaa aggtcgagcc cttttgtaag
    10861 aatctccttc tccgatatgc tcatttagct gatcatgata aagcatgtta tgattgtcca
    10921 ttgcctcttt cacaaagatc catgctaagt gaccagttgc ttcagcatca tagattgctc
    10981 ggtgatgctg ttccaagcta acaccaaact ttttagataa cacacctaat ccgaaacgtt
    11041 tgaattgagg atacagatat ctagctaatt ccaacgtatc aatgactgga ttggccgctt
    11101 caggaatacc ataccttgcg tagctagtat tcaagaaccc catatcaaaa gccgcgttgt
    11161 gggctaccaa aattgtatct tttgaaaatt caaggaacat ccgcaatact tcttcttccg
    11221 actttgaccc tcgaaccatt tcatccgtga taccagtcag atctaccgtt gttcgtgaca
    11281 atggatgtcc tggatcgata aattcatcaa agctttcgat cacatttcct ttatacattt
    11341 tgactgcggc tagctcgatg atcgtgtcat atactgcaga caatcctgtc gtttccacgt
    11401 caaatacgac gtatgtggct tcactcaatg cttcatgtgc atcattataa gcaattggga
    11461 caccatcatc tacgacattc gcttccacgc cgtataagat tttcacacct gctttttttc
    11521 cagcactatg tgcttccgga aaggcttgcg caccaccatg gtctgtaatg gcgatagctc
    11581 ggtgtcccca ttttcctgct tgtgccacaa gatcagagat gctgtttgtt gcatccatcg
    11641 tactcatatt gctatgaaca tgaagctcta ctcgtttttc cccttcagga gcataatctt
    11701 tgcgaggtgt atgtttgact tctatgatat cttgcgcatt catgaccaaa tcacggacaa
    11761 acgtatcttc ttgaatactt cctcgtacct ttaaccagct accgacactg atcgcatcaa
    11821 atacttgttc atctttttcg ccgttcgaga attttttgac gataaatgat gaagtataat
    11881 ctgtgatttt caaagtcaag atcttgcgtt ttgaacgtag ttctctgact tctttgtcga
    11941 atacgtaccc ctcaagtgtg actcttcgtt cttcttcaac gatactgatc attggtgtcg
    12001 ttggttcatc ggctggaata tttcgtccta gctggatggg tccttcaaga gctggcgatt
    12061 gctcttttct ttcctttttc ttttgttcgt ggacaactag actttcagct gcttgcttca
    12121 taaaggcttc tgcctgttct tgtttgcgtt cttcaaaaag tttcaataca cgttccgctt
    12181 gctgctcatc cacttctggt tcgatacgaa attttgggaa tccgtaactg acgaacaact
    12241 cttctactaa tggcaagtat tgttgcttca aataagaaat agctcctgta ctgtcgattg
    12301 gcaagatgac tttacgatct tttatcacag gtacctgcgt tttcaatact tgctgtgcta
    12361 gtggtgtatc gcattgctga ttttccaaag cttgggccca gtaatcttgc aagagctgtt
    12421 catcgaatgt ctggtcatct gctaagatat tcagtttgac ttgtgcaata tctttaaaag
    12481 cgatagtcag atgctgagtc aatgaccgat atagcataat aggcaaaatt ttggaaaaat
    12541 gtagcgtaaa ttcccataaa cgcgatttac gatggaccac tacactatcc atttcaccag
    12601 cagtaagcag tggatgattt ttttctgctt cctctagttg gatttgttcc aacagttttt
    12661 caaataaatc tcgtttttct gacaaagata tacgcccttt ctgatgagaa acaatgcaat
    12721 tgatcgcatc ttatcctctt ctttttcagt accttttagt atactcgttt tttctgagtt
    12781 ttacattaaa aaattggaac tgcgattcaa atcgcagttc caattttaaa agcgaatcat
    12841 cagattgtca aaaacagctg attcagctat ttttattttc tgtgttttaa cgcttattat
    12901 tcgccttggt tcaagaggat tggtaaagta ttcgccaatt cttctttacg tacttcaacc
    12961 atttcgccag ttttcttgat tttcacttct acgattccat ccactgcttt tttaccgaca
    13021 gtgattctga ttggacagcc aatcaaatca gaatccgcga atttcactcc tgcgcgttca
    13081 tttctgtcat caaccagtac ttcatagcca gcttttgtca tcgcttcttc tagttcattc
    13141 gtcaggcttg tttggtattc gtcttttaga ttcatttgga cgatatgcag atcaaatggc
    13201 gcaattcctt ttggccaatt gattccttgt tcatctgaat gttgttctac gattgctgat
    13261 aataaacgac ttacaccgat accataacag cccataatca caaatttttc tcgtccattt
    13321 tcgtctagta cagttgctcc catagattca ctgtaacgag tgccgatttt gaatatatgt
    13381 ccgatttcga ttcctcgagt aaaggcaaga acaccgttgt tgtctggtga aggatctcct
    13441 tcttgtacaa aacgaagatc ttcataagaa agcacagtga aatcgcgttt tggattcaca
    13501 tttttatagt ggaatcctgt ttcgttcgct ccagtaatgg cattcgctaa atcttggaca
    13561 taacggtctg catatacttt tacgtcatct gaaacaccga ctggtccgac agaaccaaac
    13621 tcagcaccta gatatttttg tgcttcttct tctgttgctt cttctaaaaa gtcagcagat
    13681 aaaaagtttt tcagttttac gtcattgact tcatggtctc cacgtaccaa aacaaggaca
    13741 ggttgttcat ccgcaatgaa caatacagac ttgatgattt tttgaggttc aacagaaaag
    13801 aaatctgcta cttcgtcaat tgattttacg ttaggtgtag ctactttctc caattcaagc
    13861 tgtgtttcat gcgatttttt tgatacatag tagcttgtag ccatctccaa gtttgcagca
    13921 taatcacttt cagtcgaata acagatcgta tcctccccga tttctgagat cgccatgaat
    13981 tcctttgagt ctttcccgcc cattgcgccg ccgtctccga tgatcgagcg aaattctaag
    14041 ccacaacgct tgaatacttc ggtataggct ttttcgtaat ctttatatgt ctcgtccaaa
    14101 ctttcttccg ttgcatggaa agaatatgca tctttcatga taaactctct tccgcgaagc
    14161 aaaccgaagc gaggtcgttt ttcgtcacga tatttaggtt ggatttgata aagatttaaa
    14221 ggtaatttct tatatgaatt gatttcatta cgcatcaaat cagtaaaagt ttcttcgtga
    14281 gttggtccaa ggatcatttt tcgatcattg cggtctttga aacgatacaa gtttggtcca
    14341 tatgtttcgt aacgcccgga ttcttcccaa agttctgctg gcaagatggc aggcatcagc
    14401 atttctaccg ctccgatttt ttcaaattct tcccgcataa tgcgtttcag tttttccaat
    14461 acacgatttg ctaatggcag atatgaataa atacccgctg atacttggcg gatatatcca
    14521 gctcttagca agatttgatg gcttaatact tctgcatcat ttggaacttc gcgtaatgtt
    14581 ggaatcaaca tttttgattg tctcattcta tatactcctt tatttcctca aaatagttat
    14641 cgtttgttat tataccctat ttgtcaactt tttctagaaa aagaatcgtt gaatatcgtt
    14701 ccatgtaact agcaccatca acaacattaa aaatccaaag ccagctaatg tcaatattcc
    14761 ttctttttct tgacttaatg gtttcccgcg aatcccttca aaaatattca agactaattt
    14821 cccgccatct aaggcaggaa tcggaagcag attgacgatt ccgagattca ttgaaagaag
    14881 cgccatcaaa ctaataacag ttgttacacc ttgatttgct gcttctgaag acaattggta
    14941 catcatgaca ggtccgccta atttatctaa actgaaacct gtaaatagtg atcctaaggc
    15001 tttgaaaatt tccaatgaac cgctgaaagc ttgacgcgtc cctccaatga tcttatccat
    15061 aaaacctgta ttcattgggg cttttatacc aagttggcca actttttcgc catttgactc
    15121 tacagatttc ggtgtcacgt cgacagattg cacctgacct tctcgttcga tcttaaagtc
    15181 taatgtttta cctggatttt tcgtaatgac tgttgtcaga tcattccatg aatggatttc
    15241 ttttccgtcc acactgacta cctcatcatt ttcttttaag cctgcttctg ccgcagctcc
    15301 attaggcatg atctctccta cgcgattagt gttcgtcact tgaacaccac cttgcataaa
    15361 tgccagaatg atgaataaca cgattgccaa aataaagtta ttcatgggtc cagcaaagtt
    15421 ggtcagcata cgctgccaca atttagccga ttggaactgt acgtcttttg gcgcaattct
    15481 gatttctgtt ccgtcgtttt caataatcgt cgcatcgtga tcaacagggt aggttactac
    15541 ttctgtttcg tctccattca cataaccagt gatagtcagc tcatcttcta aatcataacg
    15601 gctcaattcc atcgggatag catttgtcaa ttggattttc ttacttaaat ttatcttctc
    15661 tacgatgccg tcagaattta gtaaaagaga aagaggcatt cctggtgcca tttctgtttc
    15721 atcatcccca ttgcctgcca ttcgaacata accgccaata ggtaataggc gaagcgtata
    15781 tgttgtcccg tcttttgctt gatgcccata aattttcgga cccataccga tagcaaattc
    15841 gcggacgagg attcctgatc gtttcgcaaa gaagaaatga ccaaactcat gaacaatcac
    15901 taatatacca aaaacgatga taaatgtcag aatcgttttc atagcttttt tctcctttac
    15961 ttgttttttg tgttttttat aaaacaacga taaaacacta taattttacc atagtatacc
    16021 aaagacacca atagtatcaa tataatgaaa aataaaaacc tgcgtctttt atgaaattca
    16081 caaagaaacg caggtatact tactaaataa tgccaaataa atgcatgata ggaaatacaa
    16141 acagcaaact gtcgaaacgg tctaagattc caccatggcc tggtaagata tttcccgagt
    16201 ctttcacatc ataatgacgt ttgattgcag attcgaccaa atcaccaaat tgtccgactg
    16261 ctgaaaaaat aatcgtcatc agaagcatga caaacatacc atgattgaat agttcttttg
    16321 ctgggtatag caataaataa agaaatgcga ctacaacagc actaccgata ccaccgagcg
    16381 caccttcaat cgttttgttg ggcgaaatat caggacttaa cttatgctga ccataacgtc
    16441 gaccaaccat atatgctccg atatcagttg cccaaacaat aaatagacca aatatcaaaa
    16501 cagccaagct ttctgttcgt gcattgacaa aattctggaa acctattccc acatacaagc
    16561 tgacaagcac aggaaaacct gcttcatcaa tcgtatacgt atttttagaa ataactgatg
    16621 ttcctaaaag aatcataact ataagataaa ataacataaa tgtacttaca ttctctggaa
    16681 gaaagaaaaa ccatctttct tttggcaaaa caagtaacac tgcaccaacg gcggataaaa
    16741 ctccttcaaa acttaagagt gtcaatcctt tcatccggaa cagctcgtaa actcctacta
    16801 ccgctaagat tgctgctgta agctcaaccg ctatgcctcc gatccatata atcggaataa
    16861 acaagactag cgccacagct gctgtgatca cacgttgttt catgactgat cccctttctc
    16921 atcttctgtt ttttttacac cgccgaatcg acggtctcga ttttgataag aggcgattgc
    16981 ttcttcaaga tgcgcaccat caaaatcagg ccaaagcgct tttgtaaaat acagctcact
    17041 atatgcgatc tgccataaca gaaaattact aattcgctct tctccacttg tacggatcat
    17101 cagctcagga tcttgtaact ctttaggcaa gaaacctgtc attaaatgct tagcaatcaa
    17161 ttcatcatcg atttcttctg cgtgtatttc tccttttgct acttcttcgg caatttcttg
    17221 gacagcagtc acgatctctg ctctacttcc ataattcaaa gcaaaattca ataccattcc
    17281 agtattgttt tttgtttgct caattgcacg tcttacagca tcttgcgtat gttctggcaa
    17341 tacgttttca taacccatga catgaacttt gacattttct ttaatcaatt ctggtacgaa
    17401 cgtatcaaag aagtcgacag gtagttgcat gagaaaactt acttcatctt ttggtcgctt
    17461 ccagttttca gtcgaaaagg cataaagcgt caacactttc actccaagcc ttgatgcttt
    17521 ttttgtgact ttctttactg tttccatgcc ttctttatgg ccagcaactc gtggcaaacg
    17581 acggttttgc gcccaacgac cattgccgtc catgatgatg gcaatgtgct gaggaattgg
    17641 tccatcggta ttaaattgaa attcgctttt ttcttgaata tatttgtttt tttgcggaaa
    17701 aaaacgtaac attctttttc ctcctatact taaaagattc ccactatctt aagtattata
    17761 gcaatatcta actgtttttc ctatgaaatc attgtttttt tatttaaaaa ctgaataatt
    17821 ttttcttttt ttaattttta aataaaagaa atataaataa aaacagagga agcggaatga
    17881 tccgcccctc tgtttttatg attttattaa acttccaata attctttttc tttatcagca
    17941 gcgatcttat ctacttcttt aatgctgtta tctgtcaatg tttgtacgtc tttttctaat
    18001 ccgcgcaaat catcttcggt aatatcacca tttttttgtt gttttttata ttcatcgatc
    18061 gcatcacgac ggatgttacg aatcgcgatt tttgcatttt ctgcttcttt tttcacatct
    18121 ttagctaatt cttttctgcg ttcttctgtc agttgcggga taactaagcg aatgacattt
    18181 ccatcattgg ttggactgat tccgatatca cttgccataa tagctttttc aacatcttga
    18241 agaatgctct tgtcaaaagg tgtgatcatt aaaacacgtg cttctggaat ttggattgat
    18301 gccatttggt tcaatggtgt tggcgcaccg tagtagttca cagtaatacg gtccaaaagg
    18361 ctagcgttcg cacggcctgc gcggatttgc ccaagttcac gttgaagatt ttgtgctgct
    18421 tttttcattt tttcttttgc ttctgtcata attgcatctg ccatttttat ttccccctta
    18481 cagttgttcc gatattttcg cctaagattg cacggcgaat atttccgtgt tcatttaaat
    18541 tgaagacaag cagtggaata tcattatcca tgcttagtga acttgcagtt gaatccatga
    18601 cttgtaatcc tttggcaata acttccagat gagtcagttc ttcaaatttt actgcatttg
    18661 catctacttt cggatcagca gaataaacac cgtctacatt gtttttagcc atcaggataa
    18721 catctgcgcc gatttctgcc gctcgtaatg ctgctgttgt gtctgtcgag aagtacgggt
    18781 tgccggtacc gcctgcaaag ataacgatcc gtcctttttc taaatgacgt tctgctttac
    18841 ggcgaatata tggctctgcg atttgacgca tttcaatcga agtttgcacg cgtgtcggta
    18901 cacccacgtt ttccaacgta tcctgtaaag ctaaggcatt cataactgta gctaacatac
    18961 ccatgtaatc agcttgagca cgttccatgc ccatttgtgc tccgatttgt ccgcgccaga
    19021 tatttccgcc acccacaacg attgccattt caacgcccag ctcgtgaact tctttgattt
    19081 cttggatgat ttctttgatt actggtggtt tgattccaaa tcctgcatcc ccagctaaag
    19141 cttctccact caattttaag actacacgct gatatttcgg tgtgaccatg ttcattcctc
    19201 caatattttc tattgccatt ttaccatact tagatgattt cgacactcta agactggttt
    19261 ttcttgtttt gcttaaaaaa agggagcgca caaaccgtgc gttcccgtct gttattcaac
    19321 agaattattt tttcacttgg ctcataactt cttctacaaa gttatcttcg cgtttttcga
    19381 taccttcgcc aacttcgaaa cgtacgaatg ttttaacagt tgcgcctttt gatgcaacgt
    19441 atttttcaac agtcatatca ggatctttaa cgaatggttg gtccaccaaa gcgatttctg
    19501 ctttgaattt cttcaagcgt ccttctacca ttttttcaac gatattcgct ggtttgcctt
    19561 cgttcaatgc ttgttctgta agaacagttt tttcatgttc taattcagct tctggaattt
    19621 gtgattcgtt tacataacga ggattgatag ctgctacgtg catagctacg tctcttgcaa
    19681 ctgtttcgtc agttgttcca tctagaacag tcaatacggc aatacgtccg cccatatgca
    19741 agtatccacc aaatgcagca ttgtcgtctt tttctacaac ttcaaagcga cggaagctga
    19801 ttttttctcc gataacttga gttgcttcga tcaagtcaga ttcaattgta cctttatctg
    19861 ttttgatttt cattgcagct tccatgtctg ctggtttgtt ttcagcaaca agttctgcaa
    19921 tttcttttac taaatcttgg aacatttcgt ttttagaaac aaagtctgtt tctgagttta
    19981 cttcaacgat tgctgcagtg ttacctttca cagcaactga agccaatcct tcagctgcaa
    20041 tgcggtcatt tttcttcgca gcctttgcca ttcctttttc acgtaaaaga tcaactgctt
    20101 tttccatgtc gccttctact tcaactaatg cttttttcgc atccatcatt cctacgccag
    20161 tcatttcgcg tagttctttt accattttag ctgtaacgtc tgccatgttt aactcctcct
    20221 gtgtgttttt ctttttaaaa aaagctgctt caaaggagag tgaggcttgt cgcccagcct
    20281 ttgaaacagc tccatcattc aaaattattc tgctgaagaa ttgtcgcctt caacaacatc
    20341 tacgatttct tcgattgatg ttgctgtgtt ttcttctgtg aaatcttctt caactacttg
    20401 atcttcacct tggtttcctt cgatgaaagc atcagccatt tttgaagtaa tcaatttaac
    20461 ggcacgaatt gcatcgtcgt ttgaagggat tactacgtcg atttcatctg gatcgcagtt
    20521 tgtatcaacc atagctacga ttgggatatt taatttgtgt gcttcttgaa cagcaatacg
    20581 ttctttacgt gggtcaacga tgtacattac atctgggatt cttggcatat cagcgatacc
    20641 gcctaagaat ttttcaagac gttcacgttc tttgttcaaa ccagcaactt cttttttagg
    20701 tagaacttca aaagttccat cttcttccat tgcattgatt tgtttcaaac ggctgatacg
    20761 tttttggata gtatcccagt ttgtcaatgt tccgcctaac caacgatggt taacgaagta
    20821 ttgtcctgca cgagttgctt cttctttgat tgcttcttgt gcttgttttt ttgtacctac
    20881 gaataatgct acgccgcctt cttcagcaac gctcttcatg taatcataag ctgcatctac
    20941 taatttaact gttttttgca agtcaatgat gtagattccg tttctttctg tgaagatata
    21001 tttcttcatt tttggattcc agcgacgagt ttggtgacca aagtgtacgc cggcttctag
    21061 taattgtttc atagaaatta ctgccatgtt tgtttcctcc gatttggttt tatttttcct
    21121 cttcttatct tctaccttgc cggcaaactt tttcaagcac ctaccgacaa tccaataaga
    21181 atgtggattt gctgatgcat aaagcaccct cagctattat acagacaaaa gatccgtatc
    21241 gcaagagcaa atatactttt ctgctcaaca ttcaatagtt tacctgaaag ataaaaagaa
    21301 ttcaagaaaa acagtagatt tctctttgtt tctttattat cctttttcca taaaataatt
    21361 ttaggtataa aagacgaata ttattgggca atccttcttt tttttgttat gatacatgtg
    21421 aaatctttcg taaaggagaa agaaaatgcg caaagctcaa agacatttgc tgattaaaca
    21481 agtcattgaa gaattcccga tccgtacgca agaagaacta ttaaacaaac ttgggcatta
    21541 tggcgttacc gccacgcagg caacgatttc tcgagatatc cgtgatctaa aaatcgtgaa
    21601 ggcacctgat gaaaatggcg tttcccgctt cgtattgttt cgcgaaaaag gaactcctga
    21661 gactaggact gaggaagagg atcggctggt tcaaatgatt gaagacattg tattgaaagt
    21721 cgaacgagtc cactttttga cgatcgtcaa taccttgcca gacaatgctc atttatttgc
    21781 atctgtttta gatgagatca aacctccctc tattgtcagt acaattgctg gattcgatac
    21841 gatcatggtc atctcggaaa atgaggagga cgcaaaacga gtagaagaat ttcttcacca
    21901 tccaactgaa gtatcacttt tttaatcaga gcacgtattc ctaatatgga atgcgtgttt
    21961 ttttattttc aatattttcc ttattaaata aacctttaca ctctttacaa aaatacatca
    22021 ttttttattc aattttttca acaccttgat tgaatcacaa taatcatcat ttatattgga
    22081 tgttaacaag agcagcttct tgctctttgt gtatttcaaa aaagaaatga aaggaaatca
    22141 tatgtataaa ttattaactc ctggaccatt gacgacttct gaaacagtga aaaatgctat
    22201 gctgatcgat cattgtactt gggatcaaga atacaaagaa attacacaat ggataagaaa
    22261 aaaattattg gatcttgctc aagtatcgga aggaatatac acaacggtct taatgcaagg
    22321 tagcggctcc tttggggtag aatcggtatt aagttctgtg atcaggccag aagatactgt
    22381 actgatttgt tcaaacggcg catatggaca acgaatgatc aacatgtgcg aatgtctgaa
    22441 actgaactat accgtttatg aggtatcgga acatcaaatc ctccaagctc atgtgattac
    22501 acagctgatt gaagagaatc cctctatctc cgcactagca atggtccata gcgagactac
    22561 ttccggtatt ttgaataatt tggaagagat tgctgctgtc acacaagaaa aagatctttt
    22621 gtttatagtg gacgcgatgt caagctttgg tgggataccg attcgtgtag ccgaactagg
    22681 gattgatttt ttgatttcca gctcaaacaa atgtattcaa ggtgttccag gcttttcttt
    22741 tgtacttgct aataaagcgg aactagaaaa gcgaaaaaat gtgagtcgaa gtttgtcact
    22801 tgatctatat gaccagtacc gcgtgatgga aaacgagaat ggcaaatggc gtttcacttc
    22861 ccctacccat acggttttgg cttttcaaca agcgctgaag gaactagagg cagaaggagg
    22921 aatcgaaact cgatattcac gttatctcgc caataatcat gtactacgac aaaaaatgac
    22981 agagatgggg ttccagcctt tcattcaaga atatcagggg ccattcatca cctcttttct
    23041 ttaccctaag gactggcgat ttgattttaa tcatttttat cagtatttaa agcaaaacgg
    23101 atttgtgatc tatcccggga aaatcagcga tcatgaatgt tttcgtatcg gaaatattgg
    23161 cgagatctat ccggaagata ttttggaact gacagcaatt atcaaagact ataaggagaa
    23221 cgttaatgat taaaactgta atttttgatt gggcaggaac aactgtggac tttggctgta
    23281 tggctcctgt ccacgctttt agaaatgctt ttctagaaaa aggaatccaa ctgactgaca
    23341 aagaaatccg cgaaccgatg ggaaaattaa aatgggatca tatccaacag cttctttccc
    23401 tcccttctgt caaaaaacaa tggatagcta tttatggaca actacctcag gaaaaagagg
    23461 tggaggaatt gtatcaacaa tttgaacatt atctgtttaa agatttggtc aagcattcta
    23521 ccttgaaacc cgacacattg gaaacgatcg aacggttgag aaacgctggg atcaatattg
    23581 gttccacgac tggatttact gctcctatga tgacaatcgt agcagaaacc gcaaaaaaag
    23641 aagggtattc accagacttc atggcaactt ctgaagacgt cgaagggtat ggtcgaccgt
    23701 ttccttatat gatccataaa aacatgcagt acttcaaaaa caagtcggtt gatgaagtaa
    23761 tcaaagtcgg agatacggtc tcagatatcc aggaagggaa aaatgccgga gtgttgacag
    23821 taggtgtaat tgaaggtagt tcgctaatgg ggatttctta tgatgagtac caaaaattag
    23881 agatggggaa aaaagatcgt ttaaaacagt ctgttgctct tagattccaa caagctggcg
    23941 ctgatatttg tgtaaacaat ttaaaagagt taacagaata cattttgtac gctgacaaga
    24001 atccagaatg acttttatac aaaatcagag gtcgtgaaaa agctgtactg catcaagtaa
    24061 taagaacagc caaacaaaaa tagctcctca tatttttcgt ttggttaggc ttattaccgc
    24121 agatgcaagc ttttgaacac cgtttattcg aaaaaaaaag ggagctgtga cacgactttt
    24181 gtcacagtcc ccttatttac aaacaaacag tgtttaagag acaacctctc cactaaacgg
    24241 tgttcattca gctgactctc ggtatttgct caaaaaacga taaaatttga aaagctattt
    24301 tctcttttgt ttctctaata ctcggatcag aacgctgatt tcacattctc tttttcttac
    24361 atcttatctg gtgcatgaag tcctagcaaa cgaaggtctt ctttcaataa tacagtcact
    24421 gcataaacta gagctaagcg tgcttctttt tgttcatcat cagctaaaat cttcgtatga
    24481 gcatagtatt tgttgaatgc ttgtgctaat ttgattgcat gtttcgcaat cactgacggt
    24541 tcgtattttt ccccagcaga caagaccgtt tcagggtatt tttgaactaa tttaaccact
    24601 tcccagctgg tatcgtcatt caatgcatag tctgctgttt ctgaagggac aaatcctgct
    24661 ttttccaaca ggctgactgc acgagcatgt gtatattgca cataaggtcc agtttctcct
    24721 tcaaatcgaa caacttcttc caggttaaag tcaaatgtat tcagtcgatc gtttttcaaa
    24781 tcgtggaaaa tgactgcccc aaccccaact tgtttagcta cagcatcttt gttttccaaa
    24841 tcagggtttt tctcgctgat ttgttcttta gcagattgga ttgcttcatt taatacttct
    24901 tctaataaga cgatctttcc tttacgagtt gataattttt taccaccttg cgtgatcaaa
    24961 ccaaatggaa tatggcgcat atcgtcagac cagtcaaagc ccatttcctt caagacagct
    25021 ttcaactgtt taaaatgata gctttgttca ttccctacta catatagaga ttgtttgaag
    25081 tcgtaagtac gtttacggta caatgcagca gctaagtcac gtgtaatgta tagtgtcgca
    25141 ccgtctgact tcttaatcaa cgctggattc aggtcataag cagataggtc aacgatctct
    25201 gcacctttat cctcgttcag caaatgtttt tcttctaaaa gtttgacgat ttcatccatc
    25261 ttgtcattgt agaaagcttc accgttcaac gaatcgaaac gtacttccag cagatcatag
    25321 attttattga attctttcat cgattcatca cggaaccatt gccataattg aatagcctct
    25381 tcatcgcctt cttccagacg tttgaaccaa gcacgagctt cttcatttaa ttctggttct
    25441 gtttcagcta cttcatggaa ttggacatat aaacgaagca attcattgat tggttctgct
    25501 ttaacagctt cttctgtacc ccattttttg taagcgacga tcagttttcc aaactgtgtt
    25561 ccccagtcac ctaaatggtt gatgcggatt ggctgataac cgattttttc cataatgaaa
    25621 ccgatcgaat taccgataac tgtcgaacgc aaatgtccca ttgagattgg tttggcaata
    25681 ttaggagaag acatgtcgat tggtactgtt ccttgattcc cgatattgct atccccataa
    25741 tgttcttttt ctttcactac tgtttgcaaa acttttttgc tgataagttc tttattcatg
    25801 aaaaagttca agtaaggtcc gacaacttcg attttttcaa agttggcaga atcgattttt
    25861 tctgctaaat ctgcagcgat ttgctgtggc gcttttcggt aaacttttgc taatgaaaag
    25921 gctggaaatg caacatctcc gtgttcagct gattttggat tttctagtaa ttgttcgact
    25981 tgttccagtg ttaaatcatc tttgacagcg tcatagacag cttttgcgac aagatcttta
    26041 ttattcatct ttattcctcc taaagtgctc ccttgattgt taaacaaaaa aaatctctag
    26101 cgacggcata cttagtatgc catcactaga gacgagattt cccgcggtac cactccaatt
    26161 gtcctaaaaa aggaccagct ccatctgata tcggtagacg accgtctgtt taaaacagaa
    26221 ttctccaagt gcgcttcgtt gtatcttctt cattcggctt ccaccacttc cgaattcgct
    26281 agccaagaat gatacaagta ctctcttgtt caacaaatct tagtcttatt aaactttctt
    26341 gaattatatc agaaaaaaaa acgaaaggct agtcgtttct gataaaaaaa caatccagat
    26401 ggcctaaagg agaagaaata ccacctggat agctataaat catttcgtaa atacgaaaga
    26461 cttaacaaat taatcagcta cgataatcgt tccttcttca gaagctaaaa gattctcgat
    26521 attttctaaa gaagtgatga ttgccttagc gtttggtttt gcttcaacaa atgcaatagc
    26581 tgcctctact ttcggaagca tgcttcctgg agcaaattgc ttttcatcaa tgtattgttt
    26641 catttctgaa acagtaacag tttctaattt cttttgatct ggtttttggt aattgacata
    26701 cacattgtct actcctgtca atacgatcag caaatccgca tcgatgattt cggctaattt
    26761 ttctgaagca aaatctttat cgattacggc ttctcgtcct tctaatccag tagctgtttc
    26821 tacaacagga atcccaccgc caccaacaga gactgtgata actccttgat cgaccagcgt
    26881 ttcgatgaca cgtgcttctt taatcgagat gggtttaggg gaagcgacta cttttctcca
    26941 tccacgtcca gcatcttctt tgaatgtcac agtggagtca gcattcattt gttctttggc
    27001 ttcttcttcc gtgtagaatg gaccgactgg tttacttgga tttttgaatg aaggatcatt
    27061 ttcatccaca ataacttgag tgactaaaga aaccacatct ttatcgatcc ctttttcctt
    27121 cagtacttcg cccatggcat tttgtaacca gtaaccgata gatccttctg tcattgcaac
    27181 acatgtatct aaaggcattg ccggtgtttt ctcagaatca gcagcttgct gctgaatcaa
    27241 taggttaccg acttgcggac catttccgtg ggagatgatc aattcatctc cttgttcgat
    27301 aaatttcact aaatactttg ctgtttccat taacgcttct tgttgggctt ttgcacttgc
    27361 atccgtagac aagattgcat taccccctaa agcgacaact acttttcgtt tggccatttc
    27421 attcgtcctt tcttcagttc taccgaattt tctagataac aaaacgagcc agcgttttgt
    27481 gattttccct caatgctgac tcgttttttc gtgagatgaa tgtggttatg cttaaacgcg
    27541 agggataaac aagttaccta atgttgctgc catgatcgct ttgattgagt gcatacgatt
    27601 ttctgcttgg tcaaattgac gagcatattt gctgcggaag acttcatctg ttacttccat
    27661 ttcagtgatg ccaaagcgtt ctttcatttg ttcaccgtaa acagtgtttg tatcatggaa
    27721 agctggcaag cagtgcaaga agatcaaacg atctgtgttg tgtgtttttt ctaccatttc
    27781 catattgatt tggtaaggtt tcaacaattt gatacgttct tcgaatttat cttcttcacc
    27841 cattgatacc catacatcag aataaaggac gtctgctccg tctacacctt tatcaacatc
    27901 gtcggtaatc atcaattgtg caccggattt ttcagcaaag ccttctgcca ttttgacgat
    27961 ttcttcttca ggttgtaatt cttttggtgc aacgatacgc atattcgctc ctaaaattgc
    28021 acctgtgact aataaagagt tagccatatt gtttcgacca tctccgcagt aagcaacagt
    28081 gataccttct actgttccaa agttttcttg gatagttaaa aagtcagcga tcatttgtgt
    28141 tggatgccat tcatcagtca agccattcca aactggaaca ccagagaatt ctgctagttc
    28201 ctctaccatt ttttgactga atccacggaa ttcgattcca tcaaacatac gacctagtac
    28261 tttagctgta tcttctgtgg attctttttt acctaattgg atatcatttg cacctagata
    28321 ttcaggatgt gcacctagat cgatcgctgc agtagtaaat gcagaacgag tacgtgtaga
    28381 tgttttttca aataaaagcg caatattttt tccttctaga tagtgatgtg ggataccacg
    28441 ttttttcaag tctttcaagt gttctgaaaa atcgatcaaa tattgtaatt cttcttttgt
    28501 aaagtctttt tctgctaata agcttcttcc ttggaatact gattctttca tattaaacat
    28561 tctcctttag attgttttta tttaagatta aagaggaaat cccgacggca gctgacagta
    28621 agaccgccgt caggaaacaa attacttcaa gtcttcacgg acaagtggtt ggctcatgca
    28681 gcgtggacca ccacggcctc gtgacaattc acttgaattg atttctaaca ctttgatgcc
    28741 atagctacga agcaattcgt tcgatacata atttcggtta taagtgacta caacacctgg
    28801 agcgattgcc aatgtgtttg atccatcatt ccattgttct cgaggtgcaa cgatctcatc
    28861 tccattacca gttgggatca gtacaagatc atctaatcca agagcagctt tcatcgtttc
    28921 gtgtaagtta tctgaatgag taatcttgat atcatcacca tctggttcga ttgtaaagac
    28981 atccatctta ccgtctttac tttgaattgc tggatggata gtgaatttat cgtgatctac
    29041 cattgtaaat acggtatcca aatgcatcat tgcacggttg tttggaattt tgattgctaa
    29101 cactttttca aatcctgaat tttttgcgaa taggtggcgt gctaattttt ctaaagcttt
    29161 cgcattagtt cgttgagaaa tcccaacagc tactacttta tcgcttaaga tcaattcgtc
    29221 tccgccttcg atatgatctg gattttcacg atccaaccat acttcgattc ctttatttgc
    29281 aaatcttgga tgatgtttca aaatatattc tgtaaacatc gattcacgac gacgtgcttc
    29341 aaaagtcatc ttattgactg tcatcccatt tcccattgat gcggaagggt cgcgtgtaaa
    29401 gtacaagttt ggcattgggt ccatgtaaaa tggatagtca tcgtctgctg acagatcgta
    29461 tagactgcta gaacgtactt caatatcttt tgtacgaacc ccagccatga ttttatctac
    29521 catttcttgc gtttccattg atagaaggaa ctcatgaaga ccctctctta cagcattcga
    29581 tgcaatatga gattcatcca acattttatt caagaattgt tctttgacat tgcctgcgtc
    29641 aatcgcttct gccgctaatt tttccaagta aagagtctcg acgccttcat tttgcaatgt
    29701 ctttgcaaag ttgtcatgtt cttcttgagc aatcggaaga tatggaatat catcgaacaa
    29761 cagacgatcc ataatatctg gtgtcaaatt ttccacttct tgtccaggcc ttttcagtaa
    29821 aactgtcttt aatttcccta tttcagagaa aacgtgaata ggtttatcca tgttgtgttg
    29881 cctccttctt tttgattaca tgtataggat atcctccttt tgtaatcact ttcataattt
    29941 tgtctcattc aaaatgagac attcctcaca ttaatattcg ataattaaat aattaaacgt
    30001 ttttctattt ttaagcgtaa tttattcatt ttttattctg tatacacttt caaaaaaata
    30061 tttattcatt gaaaaaaatt ttttgaataa attaaaaaaa taaattatat tagcgttgtt
    30121 agcgtttact atatgtatta ttgttgtttt ttatacacaa atcattcgct tcaaaacaaa
    30181 ttattcaata ccattgtata tt
//
LOCUS       DAZUKC010000030        29949 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000030 DAZUKC010000000
VERSION     DAZUKC010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.031"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..328)
                     /locus_tag="ACG80T_001272"
     CDS             complement(26..328)
                     /locus_tag="ACG80T_001272"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4840933.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(353..2446)
                     /locus_tag="ACG80T_001273"
     CDS             complement(353..2446)
                     /locus_tag="ACG80T_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS4840934.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(2935..3699)
                     /locus_tag="ACG80T_001274"
     CDS             complement(2935..3699)
                     /locus_tag="ACG80T_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFS4840935.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="ACG80T_001275"
     CDS             complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="ACG80T_001275"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFS4840936.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(4704..5780)
                     /locus_tag="ACG80T_001276"
     CDS             complement(4704..5780)
                     /locus_tag="ACG80T_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFS4840937.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(5804..6397)
                     /locus_tag="ACG80T_001277"
     CDS             complement(5804..6397)
                     /locus_tag="ACG80T_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFS4840938.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="ACG80T_001278"
     CDS             complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="ACG80T_001278"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFS4840939.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(8067..8366)
                     /locus_tag="ACG80T_001279"
     CDS             complement(8067..8366)
                     /locus_tag="ACG80T_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFS4840940.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="ACG80T_001280"
     CDS             complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="ACG80T_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFS4840941.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(10121..11032)
                     /locus_tag="ACG80T_001281"
     CDS             complement(10121..11032)
                     /locus_tag="ACG80T_001281"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFS4840942.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            11152..12009
                     /locus_tag="ACG80T_001282"
     CDS             11152..12009
                     /locus_tag="ACG80T_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4840943.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(12196..12558)
                     /locus_tag="ACG80T_001283"
     CDS             complement(12196..12558)
                     /locus_tag="ACG80T_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFS4840944.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(12747..12929)
                     /locus_tag="ACG80T_001284"
     CDS             complement(12747..12929)
                     /locus_tag="ACG80T_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840945.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            12904..14343
                     /locus_tag="ACG80T_001285"
     CDS             12904..14343
                     /locus_tag="ACG80T_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4840946.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(14350..16902)
                     /locus_tag="ACG80T_001286"
     CDS             complement(14350..16902)
                     /locus_tag="ACG80T_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFS4840947.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(17016..18347)
                     /locus_tag="ACG80T_001287"
     CDS             complement(17016..18347)
                     /locus_tag="ACG80T_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4840948.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(18313..19791)
                     /locus_tag="ACG80T_001288"
     CDS             complement(18313..19791)
                     /locus_tag="ACG80T_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4840949.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(20096..21931)
                     /gene="tdc"
                     /locus_tag="ACG80T_001289"
     CDS             complement(20096..21931)
                     /gene="tdc"
                     /locus_tag="ACG80T_001289"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFS4840950.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            22401..23828
                     /locus_tag="ACG80T_001290"
     CDS             22401..23828
                     /locus_tag="ACG80T_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4840951.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(23920..24285)
                     /locus_tag="ACG80T_001291"
     CDS             complement(23920..24285)
                     /locus_tag="ACG80T_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFS4840952.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(24282..24647)
                     /locus_tag="ACG80T_001292"
     CDS             complement(24282..24647)
                     /locus_tag="ACG80T_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFS4840953.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(24839..25087)
                     /locus_tag="ACG80T_001293"
     CDS             complement(24839..25087)
                     /locus_tag="ACG80T_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840954.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            25086..25214
                     /locus_tag="ACG80T_001294"
     CDS             25086..25214
                     /locus_tag="ACG80T_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFS4840955.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            25271..26965
                     /locus_tag="ACG80T_001295"
     CDS             25271..26965
                     /locus_tag="ACG80T_001295"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFS4840956.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            27065..27238
                     /locus_tag="ACG80T_001296"
     CDS             27065..27238
                     /locus_tag="ACG80T_001296"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840957.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            27616..28749
                     /locus_tag="ACG80T_001297"
     CDS             27616..28749
                     /locus_tag="ACG80T_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFS4840958.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            28780..29100
                     /locus_tag="ACG80T_001298"
     CDS             28780..29100
                     /locus_tag="ACG80T_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFS4840959.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(29129..29593)
                     /locus_tag="ACG80T_001299"
     CDS             complement(29129..29593)
                     /locus_tag="ACG80T_001299"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4840960.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(29726..>29949)
                     /locus_tag="ACG80T_001300"
     CDS             complement(29726..>29949)
                     /locus_tag="ACG80T_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4840961.1"
                     /translation="VSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAA
                     VNLVLSILIYLPFVKVATIQERRKEALDA"
BASE COUNT         9033 a         6212 c         5170 g         9534 t
ORIGIN      
        1 tttttaccct cttttctttt tgtatttaga taactccctt atcttgcaga actggggtga
       61 ttttttcttc aatttcattt tttgaaacaa tatttttcaa atagatcatc gccgttttag
      121 gatcaactgc gaacttttcg aactgcgaac gaaaattttc tgctgtgatg atcacatctg
      181 gtttcatgga aactgcggaa gaaatatcac aatggtccag tttggcttct actcctaatt
      241 tgttcaaaac atcctcagca gacatctgtg ctgcaaaact acttcctaaa cctgcaccgc
      301 atacgaataa aatgttcaat ttgttcatct ttgttctccc ccgtatcatt tattatttct
      361 caactactga aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca atcaatcgat
      421 ccaacttctc ttcgttgttg atcaattcaa ttagttcttt catgatgttc agatgcgagt
      481 aggagtctac tgctgccaaa caaaaaataa tctttactgg atccatttca ggattaccga
      541 aatcgattgg ttgctcaatc gtagctacgc tgactcctaa tttattgacc ccatcttctg
      601 gtcgggcatg ggctagtgcc atgtgtttgc caatcacgat atacggtccg taatcattca
      661 ctgcacgaat catcgcttcg acatagcgct cttcgacaat gttttcttta accaatggtc
      721 tagcagctag ttcaatcgat tcctcccaag tagtggcttt ttctttgatc atgatattgc
      781 tgtccgtcaa aatatctttt aacataggct ggatctccct tttgttgatt tctaaattgt
      841 ttgtttcgaa taatttttct aacttgccat agatgattcg atctactcgg ccaccacttt
      901 ctttcatgac atctattatt tgagtgaata actccgtcga ttcatgactg ttaggctcta
      961 accgctgtag ttcttcattt cttttcaaaa agtcttctat aaccaagata ttcgtctcag
     1021 ttaaaatcgg atcgacaact aaaagcggct tatttcgata attcaattga aaggtgctga
     1081 aaaccagatt gacatccaat ttatctacta attcgatttc ttttgaactt aaaactgcag
     1141 taatctcgat ctgaggaaac ttttctttca gattctctgc taacagattt ccagtagcca
     1201 tcccatggtt acagacaacg actgatttat aaacataatt caagtctcgc ttgattacac
     1261 tggcgatcgt cgaaaagtgg atgacaagaa aagctatctc gtcctccagt atcttgccac
     1321 caattacttc ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt gggaaataat
     1381 ttgttcggat gttttctttc aatggattca tgacttggat atgattcttg attcttacaa
     1441 tcagtgcagc catgtgctta tagagatttt cgaataaagc ttcttccttt agatggaaag
     1501 gtacgtgtgt ctcgttctct acaaattgga tcaattggat cgttagtaat tgagcttgta
     1561 cccactcgat tgaattggag atatctctag cattgaacgt ctcaatagtg aaacgaagat
     1621 aatttctttc tacttgcggg acattgtcca attgaaattc ctcgatcatg tggtcaataa
     1681 atgcagaaaa gctactgtct tttatttctg tatttttcca attaatggca gagataaatt
     1741 catgtttcct gattcgctgg atccagatca atgtaaaaag caggatttgt tttttataaa
     1801 tatcatcttc ttgtttcaac aacttattgg aatagatctc ctgtattttt ttgatttcca
     1861 ccaaaggaaa ataatggaga aagatttttt gagccggtgt taattgtgca tcttgattca
     1921 taaagaagtc ggtcattccc agattctgat tgataaaatc atagatcatc gtacggatag
     1981 atcgttctga acctttataa accagtcctt gctttccata actgacgatc tctatcccat
     2041 aactgaccag tgttgctcgt aaccgtctca tatcttcatc taatgtactt ttagagattt
     2101 gaaattcttc ttcttttttg tttagatgga tcgggattcg gctgtaagca acggctaaaa
     2161 ctagatcaaa aatacgttcg cgccgattca aaaagccgtc tttcacatct tttatttcag
     2221 tttctaatcg agcttgcttt tccttttcca gcttcaacaa aaaacctttg ttgcgaattg
     2281 tacggatctc cggaagcttt ttactgctca accagatatt cgcttcaaga acttcctgac
     2341 ggatcgtccg ctgactgaca ttgaactcac ttgctagatg ttttgctttt actggatatt
     2401 gggctgtcat gattctgtga agtatttcaa ttgttcttgt tttcatagaa atgatctcct
     2461 tctgatttca agtatatggt tccgcttaca taataaaaag tagatatttc tgccacttga
     2521 agtggaaaga aagtaagcaa agcaaacaaa aaataagcca tatgcttttt catctcctca
     2581 acgaaaaaga catacgccta ttattacaaa aattttgccc atctatacaa aaaagaggtt
     2641 gtgaaaaagc tgtcctgcat caagtaataa gaacaactaa actaaaatag tgcttcctat
     2701 ttttcgtttg gttgttctta ttatcgcaga tgcaagcttt taaacaccat ttagtggaga
     2761 ggttgtgaca gagatgttca gccttgagcg ataagaagcg attttgaaaa atcgcttccc
     2821 atattttaca aaatcgtgcc ttattgccga taggctatct ctggcacacc gtttattcag
     2881 aaaaaggagc tgcgacaagg cttttgtcac aactccgttc ataaatattt cctgtcaatc
     2941 cagcataatc ggctgaataa aatcggcttg ccatcttttt tctttctcag aaaaatcaaa
     3001 ataccatatc tgagaaaaat actgtcgttg tgtcttactt cctgagatca atcgcccatc
     3061 ttttcgatcg actgaataat cacagcatct gaaatcaatt cggatggcaa aactattttc
     3121 gtggatctgt cgataatttc ctacttcctg aatagttatt tttttagtat gatttcttac
     3181 accgtctgct ttgttttttt gcagaacttt ctgatgttcg gcaaacagct tgtctgtata
     3241 acagcttctg gctttttcag gagtttcttc ttcccaagcg gattggatct tcatgaatat
     3301 cttttggatt tccagaaact gtgctttctt ttctttagcc gttcctgaca tgcgggacaa
     3361 tatctccgct ttcagccttt tcttttgtct tttattcttg acgatagaaa aagcagaaac
     3421 ccctcctact gacaaaacag ctaatgcaag aaatcccgtt tgctcataac tagaaggacc
     3481 tgtacctgga ccataatacc cataagaatc tctgccgtaa gaacgactgc caaaataact
     3541 gcccccatat gaacgaccac caaatgaacc tctgcccgaa cttcgaccgc tagatgtacc
     3601 tccaccatga ccacccgcga cagcgaatcc ttgggtcggc agagcaaaca aacataggcc
     3661 cactaacaaa atgattgcta aataattgat ttttttcatt gtcttcctcg ttatctttta
     3721 ttacaaaaaa ctatttttat gatagccttg ctaaaaggaa gagtcaataa ccagatgcat
     3781 aaactgtttt tttagcaatt ttgtcttatt tagtcattga gtaacatcgg acaaatgtat
     3841 gtgtcggcag tccataattc ctctatcttc agcatgaaca agcttgcaga caagagatct
     3901 gcgcttcctc caggagagat ttggttttcc tgacaaaagt gcaacagtgc tgtaaatgga
     3961 cgatttggta aagcactttg actagcagca tgagcaagtt tttgcatttc cactaacgtt
     4021 ttctgatcac tgcgatgcaa tacgcatgtg tcatctagcg aactcatcaa aactaacaac
     4081 atatgcattt gtttctgtac atatgaatca ttatctagct ccttagctgc ttgaattgca
     4141 agcctgatat gcggataccc taatatcgct tcttcatatg caccatttac gccatatttt
     4201 tgcttcaccc tatgaccatg agtggttttt ttatgacgat attttgtatc aggaaaagca
     4261 gcaagcttgc tgacatcatc aagtattttc gataaagaaa aaggctgttc catactgatt
     4321 ttttgtgcaa tgacactgat caatagcccc attgcccaaa tcgctccttt atgggtattg
     4381 atgccatttg tagcaatcag cattgccttt tccgcttttc gcccaatctc agcaattctt
     4441 tctcttaact cctgatcaat agcatgatgc catgctattt gagccatttc ttcaaaaaaa
     4501 ggagttagcg tcttagcaga cttaagaaat aaagcatagt ccatatcgga atgtgcaccc
     4561 gcatccagtg catccactaa tccaggtttt ggacttaatg ttacttctgc gatcaatgct
     4621 tcctctacca acgcagcgag ttgctgagtc actgcttttt ttcgttcgtc atttatcatg
     4681 acactcactc cttcttccta ctattacttg ctgaaacctt ttttgatagc tacccaaatg
     4741 atatgaagta accagcttaa agcaaatgca caacttgccg tcattagata agctacaaga
     4801 atcatgactc ctgaaggtaa ctgcagccaa ctatctttaa acaataataa cagtacttgg
     4861 aaaacaaata cattggctaa atacgtacga taagcataaa cagacaacca tttgaacaat
     4921 ggcaattttg gcgaatcgtt tacaatcatt ttggaagcgc ctatgaagac agcaaagatg
     4981 atgactaaac tgtataaact ttggatcgta ttcaagtaag gtgcattcgc aaagctcaag
     5041 tctcctggat gtgcgagcaa tgaacggaca gaaaagaacg cgataactaa ggcaacaggt
     5101 aagaaaagat aacgtactct gtccaaatat tgatagactt tttcgtgata aacaaatgcg
     5161 gctaccccat aaatcatgaa actagcaaca aaccgatcta ataagtacca gctgtcatga
     5221 taaggcccag tcaatacttg tgtttgatag aacagatacc atgcgacata taaaatcgtt
     5281 gaaccaatca atactggcca tacatatttt ttattagcaa atactttgta accgagccaa
     5341 acaaaaaacg gcatgaacaa ttggatctgc agcatcataa cggtatacca taaatgaggc
     5401 gctccatcac ctgtgacata tttcagtaaa aagcttgcag tatccgtata cggagtttgt
     5461 tgttggactg ctggaaatag ccaaagataa gccgtcgtcc atagaagata cggaacgact
     5521 agttcaaaaa acttttcttt caaaaattcc caataagaca aatgctgact tgtttttacc
     5581 atattataaa caattgcaaa aataaacatt ggtgctgtgt atttaacgat gacgtaaaag
     5641 gtaccaagaa aagctgtatt tgttgcgctg tggctgtttg ccaaagcaaa gctcaatatc
     5701 gtttgcatca taacagccgt tacagctgat actttctgga agtcacttac gttgattttg
     5761 atttttttgt tttcaaacat ctctttctct ctctttttct ttattaccaa agttctttgg
     5821 ttaatttagg tccttcgact gttttcaata gaattttttt ctctggctct ctcaaatact
     5881 cttttagtga aaaaccgcct tgccgagtca ctacttgggt atcgaattgc tcaaaaaaag
     5941 caggatgcga ttgaattacc tgaattggca attcaatcgg tgaatttgcg tacagcagca
     6001 aatcaaaatc actggtttct tttactgccg gaatcccagt cgataattca aatcctaagc
     6061 tgccgccgat tccccattta catcctgata atatgtttct agcagcatga aattgctgat
     6121 aaacaggata atccatgatt ttctcttgtc tatacgattc ttgttcgatg atgtcccacg
     6181 gatgaatgac ttgtttgacg gcatgttttg agatttctgt tgaccatctc tgattgcggg
     6241 caggtccacg taaaccaatc ggtagtattt ctgcgtgatc cgtcgacgca cgacgaactg
     6301 ttccgaacat cttttcagtt agaacgaccc attcaggcaa ggtgaaccct tctatttcat
     6361 gtttatcaaa aaggacaata tcatgtgctt gaatcatttt ttatgcatcc cattcttcgt
     6421 ccattttggc acggacttta tttgtttcga cacgtccgaa tgttttggct gtttcgtttg
     6481 tgtatcgatt tgtcagcatc gtgtcatttg tattgcggac atcttgaatg gcttcattga
     6541 ttgcttcgat gactttgctg acattttgtt ctgaagcagc ttggtcggtg atatcttcta
     6601 ggaaatgata gatcgctcct agttttgtat aattattgat atcgtaagcg atagctggaa
     6661 ctttttcagc agctgcttcg atttcagcaa ccgaacgttt tgtcacacgt gcagcacttt
     6721 ctttagacat cgcttggacg gtaatttctt tattgtttaa agcaatcaag cggcttgatt
     6781 gtaatccatg tgctaaaaat gcacctgaaa tagcatttcc tgcaatcaat ccaataactg
     6841 gatgtccagc ttgacggagt tttgcataag cagcagcact attagccaaa gccacatgga
     6901 taccgatcaa ttcttcgtta tagccgtatg cttggcttgg tacatcgatc acaacgatga
     6961 ttggccgttt cacttttttg tctttatctt cttcgtaaac gtgatccaaa atttgtccca
     7021 tgcggaaacc ttcaactaaa ccaacttcac cgtttcgtac acggtacatc gggtttttag
     7081 gatcaggtac gattgatgtc acgatggctt cttctatgaa tttacttgat ttaccataat
     7141 acacagtact aatagaagaa gttggatttt tcatgccagt tagtgccgca aaccaatcgt
     7201 aaccaacact tgctgcagct ggttcatttc cttctgctac ttctggtaag tcaattgatt
     7261 ttgcttgatg gttagcaatt gcttcattgt aatcgtcaat ggccattggt tttgaaagat
     7321 ctaaagaatc aagtaatgat aaataaaatg ctgagctttc actgcgatgc gtgtcttttc
     7381 gttcgtccat tgctgcgatg acagcagctt tgattgcttc tacactatca gtcactaatt
     7441 catcaatgat ttttgattct acccgttgac gagatcctaa tgtattccaa atcaagtctt
     7501 tgtcactaga atcaaattct cgaacgcctg cttcttgttc aataacttct ggtccattca
     7561 aaccgactct cgcttttttt gttgcgatta aataactcat taatgctgaa gtaatcgaca
     7621 taccgccaaa tgatcctaca cgtcctggaa tcaaaccgat gactggtaca tattgtttta
     7681 gagcaatgat tacatttccg atttctgaaa tagataacag tccgtagttt gcttcttgca
     7741 aacgaacacc acctgtatct agtacaatga ctggaaagat ctcatttcct tttttatttt
     7801 cttctaacac atgttcaagt gcagcaacga tttttgcacc ggaaacttca ccgataccgc
     7861 caccttggaa ttttccttcc atagcgatta ctactgcttg tttgccattg atcgtgccac
     7921 ggctgacaac gatcccgtca tcactttcag ggacgattcc ttgtgctggt aaacttggcg
     7981 cgatgatttg atcgaatgga tcaatcaatt cacgtccatt gtcaagtaga gcgttcactc
     8041 gttctctggc gtttaattct acaaaactat ttttcatctg aaagttcctc cattgcttgc
     8101 gtcaatcgta ggtacacaac tcctggtgta gcaccgaagt cattgatcac gatatctgct
     8161 gaaattggat aacgatcaaa aaatctcgtt aatacattgt tccatacttc gccgaagcca
     8221 tcgcttcctg tacaaatttt tacagtagat tctgtttttt ctgttggaaa catcaagatt
     8281 tccaaatctc ctgaaccgac cactcctaca tggattttct tattgatcgg ctgtttcgtt
     8341 ggatatttat aagctaattg ttccatggat ctctcccctt tttgtaaaac agagcataca
     8401 cgttctgctt attcgtcttt cttcttccat ctgggtaaat ggcgacaaaa ggtttttctc
     8461 gccaatttta tgaccaagtt ctgaatttca ttggtggttc atacaggcca tctgaccatt
     8521 cgatcaaatc atcgatggtt tgtgcagcta ataatgaacg tttcgcttca ctacgtttga
     8581 tgcctagatc ttctggtaat ttcacgatgc cggctgcacg caagctatct agtgattctt
     8641 tcgtgctagt cattccaaga ggtgtaacac cgccaattgc tgcaattgct gcttgtcgtt
     8701 cttcttttgt ttttgctttg tacaaataag caattccttc ttctgtcacg atatgtgtag
     8761 tatcttcgct ataaatcatc actggtgcat tggctaagcc tgcttgtttt tgaacagcta
     8821 gtgcatctaa ttcttcgaca aagactggtt ttttatttga accatatgtt tcaaccattt
     8881 gaacaactaa tttttggcct ttccctaatg gatcatcatt gtcgcgaagt gattgccatg
     8941 ctggtgtaga atgacgacga ccgccaggat tatgtcccat gttaggcgca ccgccaaaac
     9001 cagataaacg tccttttgtt acggtagaag agtttccttc gtaatctaat tgcaaggttg
     9061 aaccgataaa catatcgatg gcatattgtc ctgcagcttg tgataatgtt cggttactgc
     9121 gcatcgtgcc gtctttaccg acaaaaaaga catctgggcg agcagcaata tatttttcca
     9181 taccaacttc gccaccaaaa ctatggatgc tttcaaccca acctgattcg atggctggaa
     9241 tcatcgttgg atgtgggttc aatacccagt tcttcgcgat tttccctttt aaccctagtg
     9301 attcaccata agttggcagt aacaattcga tagctgctgt attgaatcca ataccgtgat
     9361 tcaaagattg tacgccgtgt tctgcgtaga ttcctttgat tgccatcatt gccattagaa
     9421 tttgcaattc tgtgatgttt tgaggatctc gtgtgaataa agcttctaat tgataaggtt
     9481 tatctgcttt cacgacaacg tctacccagt caccagggat atctacacgt ggtaaagaat
     9541 cgacaacttc gttaacttga acgatcacga taccatcttt gaaagctgca gcttcgatga
     9601 ttgttggtgt ttcttccgta ttgaaacctg tgtacaagtt gccgtcttta tctgctttat
     9661 ctgcagctac taaaacaaca tttggaatca aatcgatgaa taagcgtcca taaagttcca
     9721 aataagtatg gatatcacct aatttcattg tgccgtcttc aatcatttgg gcgacacgca
     9781 agctttgtgc accagcataa gagaaatcga tcttgcttgc gattcctttt tcaaaaatat
     9841 tcaaatgttc tggtcgtgag atactagaca tgatcaaatg cagatcattc actttttctg
     9901 catctacttt ttcaagactt tctgaaagaa aacttgcttg tttttgattg tcgccttcta
     9961 atacaacttt gtcgccggga gcaatcagtt tttccattac ttctacgatt ttttcagttt
    10021 ctacatactt gccattgagc aagttgctga ttttttccaa tcgctgcgct tttttttcgc
    10081 gtttagaagc ccagttgcgg tttggttttt ccataattag tcatctccat ttttgatata
    10141 gaaacgtggc gtcatcaata ccatgttggt ctagattgat cgtacggatc cctttatcta
    10201 caccaaattt tgtatgaatc agctttgtca aagtattgcc ggggggaaat tcaatcgtca
    10261 gatccattcc taattctttt gccaagtcca tcatttggtt ccactgcaca gggaaagcta
    10321 ggttttccaa caaatcttta cgaatctctt ctgcgtcctg agtggctctt ccatccgtat
    10381 tttttatata caaacatcta gcatcagata gatggacaga ctgcatatac ggcttgaatt
    10441 tcttgaccgt atcattcatc aagagacagt gagatggcac agggactttc agcagcttcg
    10501 ctgtttgcgc ttggtgccgt ttggcttctg ccaacgtaac ggataaacct tcgatactcc
    10561 cagataacgt atgttgcatc ggacaatttt catttgacag ataaactgga tggtcaggat
    10621 caaatgtttt tgctacaatt tcctcagctt cgcttctcgt cacacctact actacgccca
    10681 ttgcataacc gcttggataa gcttctttca ttaagactgc gcggtgatag actagttccg
    10741 ctgcattctc aaatctcaaa gatcgacaag caacagctgc acaaaaagca ccaatcgaat
    10801 gtcctgctgc aatatcaggc tgaataccgt ttttttccat ttcatctaaa taaaatactg
    10861 cttttgtcag taatgctagc tggataaaaa ccgtatcttg atagttttct tctttatcaa
    10921 ctaaccgata ccccgtcaca ttttctaaac gttccatgta catttcaggg atatccgcta
    10981 acatccctaa cttctgactg ccttgtccag gaaataagaa acctgtactc atgagcctcg
    11041 cctcctcagc acttactttt tccactgcta tcttagtcaa tttctgctat aataaaaaat
    11101 gccaaaaaaa gaaaaaacca taacaaaaag gtatggatgg gatggataat tatgaaattg
    11161 cttcatctgc agtattttaa aaaagtattc gaaacaaaaa atgttaccca agcagccaaa
    11221 gaattattta tttctcaacc tgccttgagc cgtgcgatca aacatttgga aaacgaatta
    11281 ggtgtccctt tgttttacca tagcggcaga aacattgaga cgaccgttta tgcagaagaa
    11341 ttttatccat atgcagtcaa gacattagac acattagaag aaggaatcaa attattgtct
    11401 tcggtcaatg agaagatcgt tacttccgtc atattgtatt tagaggtagc ctctgtttct
    11461 attccgaact tggtccgaat ttttatggaa aagcatcctg atatccaatt aacgatcatg
    11521 caacatcatt taccagatga tattaccgat cctgtactgt acatcacttc ggagatgaag
    11581 cctggcatga ccaatattcc tatcatgaaa gaacctgttt atgtcgccat tcctaagaaa
    11641 catcctcttg ctaaaaaaga acatctgcaa ttagaagata tccaacgcac ccccatcttg
    11701 atgctgtcaa agagcaacgc cttccgtcat acactggatg cagcacttga agcgaaaagt
    11761 atcgaattga cgatcagttc gacgacagat gatcctgcga cattacgttc tattgtcaag
    11821 caaggattag ctgttagctt tttcccaaaa gtttcttggt cttacgataa aaatgatcct
    11881 ttcgtcttgc gggaaatcaa agatttgccc ttgacacgaa caatttattt atcttctagc
    11941 ttcacagaag atcatccttt gatcaaaatg attgcaaaaa cgatgcggga attttattta
    12001 ggaaaataaa aaaaaaagag ctgggacaaa attcttgtcg cagctctttt ttcgaacaaa
    12061 cggtgttcca gaagtaattt cttagaaaat aagccgaaat ttcccaaaaa tttgagaagc
    12121 aattttcgta aattccttct tatttcttga agctaaacat ttctgtcaca acctcttctc
    12181 tattgtccta gacattcatt gcgttgattt aaaacttcca tcaatcaaat ctgctagttc
    12241 atctagcttt tctattttac gtagatcaat agagatccaa tgttctttat tcatgtgata
    12301 ggctggaaaa tacccctctt ttcctcgcaa aattccgcca agttcaggat cgaccttcaa
    12361 atcaagaata ttgatgatcc tatggtcact tgcatcatca tagatttttt cttctggaac
    12421 agacatcagg accccaaacc attttttgct tttttgatgt ctgaatacgc aataatcagg
    12481 aaatttttta aaaatatatt ctggagacaa attgtatgtt tcattgatgt gatcgattag
    12541 ttcttttttc gttagcatct gcttccatct ctttcttatt gattcgcttt attcaaagat
    12601 accacgtaag aatagaaaaa tgaatacaag tagttgctaa tcaaatactt cgatattttt
    12661 cttttttatc aactcttaaa tgttcaatcc gcctgtgttg gtcttgctac catctactat
    12721 taaaatgttt attattgttt gcccacctat tttttgattt tagtggtatc atttttcaag
    12781 ttcatgatga aattcttatc atcagtaaat gctccagaaa aataaacaat gattgaaaaa
    12841 tcagttgaaa cttgaccacc gtttattttc tccgtattct tagaatagaa gggagtctga
    12901 ttgatgtatt cactaatgaa aaaaatcatt accgaaaaag atattcgacg tcatgtttct
    12961 ttagtagaac aattgctgaa tcatacaaag ataaccgtaa atgaattagc tgaaatcata
    13021 gggacaaccg aacgaacgat tttttctgac cttcaatcta ttcgctctca gctgcccgaa
    13081 ggctgggaca tcatcagcga tcaagcagga atcagtttgc aaaaccagca aaatttatta
    13141 acaaatgacc tttgggagat ttttttcaaa caatcagtca gtgtcgaact gctgaaaaat
    13201 ctgcttttta caaaaaaagt tgctgtccct gattttctag ccgaccatgg gctttcttat
    13261 gggacattga aaagacatgt gactaaaatc aatcagcgtt tagcaagtta cgacttgcag
    13321 atcgatttga cgaaatatac agcttgtatt cttggaaaag agcgtgcgat tcgtactttt
    13381 tatcatcgat tgctgattcc tttcactcat aacaactatt ttttcgagga ttattcgatc
    13441 catgaatccc attattttca atttttaaga aacctcagtc aaactgaact agcggttgaa
    13501 acagaagaga ttttcggaac atgttggttc tttatcaata cgatccgtat aaaagcgaat
    13561 tgccgattag attcttctat ccataccaac agcacactct cctccctgta tgactctgca
    13621 ttgaaaaaat tgtatttaac ggaaggtatt tatcttaaag acactgagct ttcttttgct
    13681 tctttctgct ttctggaaag ttggaattac aacaataact atggacaaga aaccgctcgc
    13741 tgcctgcatc attctccatt tttagaggtc ctcgaaacgt ttgtagagga actagcttca
    13801 gagctgtctc ttgatcaact aaaaaaaaca tctctgactg ataacttggc tctcctcata
    13861 ttaaaatatc atgaatcgcc gatattgatt gaagcattgg accgtcaata ccatcgtttt
    13921 ttagaaacat atgatcagca atatgcacac ttgtatcaac agaaagagaa tttactgaac
    13981 gatctaaaaa aagaaataga aatcaatgaa ccaaattact ttctccaact cttgtctcta
    14041 ctgatccaac aaacgatttt ttctatcaaa ccgaatttgt tcaatgtcta ttttattttc
    14101 caaggagaac cttcatggaa ggcgttcttg cagcaggaat taaaagatta cttaggaaaa
    14161 cgtgtctgct tcttacctat cgaactaacc gatctttcca ctatttcttt tgaaaaaaca
    14221 gatatcttga tttccaattt ccctctagat tctctagata tccctattgt atacatttct
    14281 tctattccaa cgaaaaatga gctcaatcgt ttgactgaac tcactttcaa aactttttta
    14341 tagtaacggc tagcgactgt attctctagc tgttttttct tttttgcgaa acagtttgtt
    14401 catgatccct ttttctattt ctacaactaa gaatagaaga atcacatttg ctccaatcaa
    14461 taactgttgt gtcatcgtta aacttactgt accgatcagc tgctggccga ctggtagaaa
    14521 aataaccatc ccttgcaata aagcaaggat acctaatgaa agaaaaagcg ctttgttttg
    14581 caaaagacct tggttcaatg acggcttgga caactctctg caattgatca tgtaaacagc
    14641 ttgagctaag acgatatttt gcagaagaat ggtttgctgt aagctttgcc cttcaaactg
    14701 catagccaat aaataggcag gaaccatgat cagtagggag acatagatga tccgaaacaa
    14761 atgataacca gataggattc cttgctttgg atctcttggt gggcgcttca tgatttctgg
    14821 atctggcgac tcgaacccta gtgcataaga taaagtgatt gtcgttacca tattgaccca
    14881 taaaatctgt actggggaca aaggtagcgg gcgattcagc ataagtgccc aaatcaagat
    14941 caatccttga gctaatgcag ttggcaagaa gaaattgatg gttttttgca aattatccaa
    15001 aatccgccgg ccctctttta cggcttttgc aatcgtatgg aaattatcat ctcctaaaac
    15061 catatcagct gcctgtttgc ttacttcact acctttgatt cccatagcaa ttcctacatc
    15121 ggatcttttc aaagcaggtg catcatttac gccgtctccg gtcattccga cgatttctcc
    15181 atttttctgc aaagctttaa caattcgcag tttatgttct ggtgtcgttc gagcaaagac
    15241 atcgactatt ggcacttgct gaatcaactc ttcatcagac ataagatcca aatcgatccc
    15301 ttctaaaact ttggcagtat gttttaagcc aatttgttcc gcaatcgctt ttgctgtgtc
    15361 tttgtgatcc cctgtaatca ttttcactga gatccctgct tgttggcatt cctttacagc
    15421 tttgactgca ctttcttttg gcggatcgat caatccggcg atccctgcaa aagttaattg
    15481 ggttaaagtc tcatgcgtga gttcctgttt cgaatctact tttttataag caaatcctaa
    15541 aacacgctga ccttttctcg caaattctgc agctgtctga ctccatgctt cttgttctgc
    15601 agaagaaaga gttgatttag caaataatac ttctggtgca cctttgacgt aaatcaccgc
    15661 tcctttttcc tccgcatgca tcgttgccat atatttgtat tcagaactga acggaatctt
    15721 tgcttctatt tttttcaaag gtagattggc tgtttctgtg tgtcgaatca gcgcaacttc
    15781 tgtcggattg ccacttaaat tttctgtttt ctgttcttct tttgttttta attcctgaca
    15841 gtttttcata atggacaaca cgcaggcttc ttcttttgca gctacatcca caactgtcat
    15901 ttcatttttt gtcaatgttc ctgtcttatc tgaacagatc actgtcatag aacctaatgt
    15961 ttctaccgaa ggaagtcctt tgatgatcgc cttttctttt gccatttcat gtactccaac
    16021 ggatagaatc atggtcaaaa cagctggcaa tccttcgggt accattgaca ccaccaaagc
    16081 aatcgtagcc gaaagaagga tatgccaatc cataccatat cgaaatgtcg taaaaataac
    16141 taagaatagg ctcaaacaaa gaatgccttg aaaaatttgt ttgtttaatc ggttgatttt
    16201 tttaatcaga ggcgtcgttt gttgttcgac cgattgcaat gcttgattga ttttaccgat
    16261 ttctgtcgtg tttcctgttt ctacaacgac accgactgca gaaccagatt gtacaagcgt
    16321 tcctgaaaaa cccatatttt tctggtcgcc gatcgattct tcttctgtca aaacggcatg
    16381 acttttttca gatgcttctg attctcccgt caaaatcgat tcttccacta ataaatcatg
    16441 tgcttcgatt aatcggatat ctgctggaac gacatctccg gcacgtaaga caatgacatc
    16501 tccttttacc agttctttcg cagggatttt ttcttgcatc ccgtttgata gtactatcgc
    16561 ttcttgtccc atcagctgct tcaaaccatt caatgattct tccgcttttc tttcttgcca
    16621 gtaactaacg agtccattga cgatcactac gaataaaata atacttcctt caatatattc
    16681 actagaagca aatttcaaaa aagaggcaat gatcaacacg atcatcagca gatcagtaaa
    16741 atgctttgct atttttttcc atcgttttgt ctgctgcctt gcttttattt cattatatcc
    16801 ttgatcagtc aggcgtttct gtcgttcttc ttgcgacaag ccttctgttg atgtttgata
    16861 gtttttcaat acatcttcta ccgtcaatct gtaccagttc atcctatccc ctcctgttta
    16921 attgatactg ttattatacg cagggaagtt ttttcaggcg tatcgattat ttcagggggg
    16981 tctgaaataa ttcgttttgc aaaatgtatc tgggcctaac tgatatttct attgcttatt
    17041 cttttatata caaaatttaa gaatacaaac cagataacca ataaaaaagg aataaagcgt
    17101 gtcgtgcttt cgaataacaa gctgaaagca attgaagtaa aaaacaccaa caaagcaaga
    17161 tctttatatc tttgtgtagt gttccgtttc ttttctttaa taaattgaac atgtgcacac
    17221 aagatcgaag accacacaaa tagaaaacaa ctcgttgtca tgcctgccaa aagctgaaaa
    17281 aacaatcgac tatcagctac aaaaaaatgt agacaaagac cggcaaaaaa taacattcct
    17341 gtaattaaaa taccgtgtaa aggtacgcct ttcttagaaa gcgcacaaaa ccaatggggc
    17401 aaactttttt cttttgctgt ttgatataaa atcctgctag tactgtagac tgcactattt
    17461 gctgaggaca aagaagcaac caaaatcatt agattgacaa ttgctgctcc gccttggcaa
    17521 tggatcactt gcagcatagt cactaaagga ctttctttta aatctaaaca atgccatgga
    17581 acaacaaata aaatcgacaa aatcgttcct atatagaaaa agagaatacg ccaaggcaga
    17641 cagttgattg cttttttcaa attcttttgc ggatcacttg tctcgccaac ggttaagccg
    17701 atcatttcaa ttccagcaaa actgaataaa accataggaa atgagactac cattccagca
    17761 aagccaaacg gtagtaattc atcgatctgt ccaaaatttt ctttgattcc gatagactgg
    17821 gtatgaaaat ctgagacact caatcctata cccaataaga taaaaaggaa aatccccaag
    17881 atcttgatga ttgagaaaaa agattccaat tcaccaaacc acttgactgc aacaaggttg
    17941 actgccatta agatcattag tgcgctacct ccgcttatca aagctggtac atgtacaaac
    18001 cagaaatgga tatacaatcc tattgctgtt aattccgcaa gtccagctcc aagccagcaa
    18061 agccaataag tccaattgac aaaaaatgcc cactttttgc ctaaataata gtcaacgaaa
    18121 tcactgatag agttaaatgt actatttgct aaaaataaat cacccatggc ccgcatcaaa
    18181 ccataaatag ccaaaccgca tatcaaataa acgagaataa cagctggccc tgctgatgca
    18241 attgcctttg ctgaacctaa aaataatcct gttccaattg cgccacctaa tgcgattaac
    18301 tggatatttc gattagttag agtccgtttc attgccggtt tttccatcaa gttcaccttt
    18361 ttctttttca agcagcatgg cgacttcctg ttcccaagaa ggtttcttta ttttttcaat
    18421 acagagtggt actacaaaca aaatgacact gatggctgtg actgcaatta gattgatcca
    18481 tgttccagct ttcatcgtca gtccgacaga aaatccgatt gccgcaaata aaacaataac
    18541 gacagtccaa actccaagat tgctcttttt ctcttttttt ccaattctaa atggtcgatt
    18601 caactttgca tgtgtataac gcaatctcaa aactgcgatc cccatgatga catagacgat
    18661 acagtagata acagtagttg aatttacgag catcaaaaat gcttgattga cgccaggaat
    18721 cagcaaataa aaaagtgcaa agattgagat aatgacagac tgagtcaaaa taacggattt
    18781 ggatacgtca aattgattcg tcttccaaaa attgaatttt ggtggatacg caccttttct
    18841 agcactttct gtcactgtct ttgctggtcc tgaggcccaa gcagataatt ggactgctac
    18901 accaatgaag acaagtaaac taaacagatt cacaataaga tgtggcaaac caagaatttg
    18961 gcaataaatc gaaatagatt gcgcgatatt attcaattcc atttgtcctt ttggtacgac
    19021 atttgctaca actaaagcat tcaaaatatt gacgaaaaat ataaaaatta acgcggcaaa
    19081 tactccgcga atatacgttt taactggatt ttccaatcga gtgatataca cagaggacat
    19141 ttcgatacca gtaaaaataa acatgatcgg cgcaaaataa acaaatgagg atgcagtcgt
    19201 agtatctggt attaatttgt cccatgaaaa actgcctaat gttccggttg gtacgatccc
    19261 aactttgatc cacgcagcaa accctaataa gagcatcatc gtcaaaggca cgtacaatcc
    19321 taaccaaaca ccgacttttc cgccgatttt tgccatatca aatttcaaat tcaaaaaagt
    19381 gatgatccaa taaacgacta aaatgacaac taatgtaaat ttgctgttca aaccaagcgg
    19441 cacattatcg ataatattac caaacaacgg agcgagtgct gaagcgacca tcaccatccc
    19501 aggaaacatt tggacccata acagccatga gacaacgaac ccccattttt ttcctagagc
    19561 attcgatacc caaagttctg gaccaccttt ttggggaaac attccagcaa attcaccaga
    19621 gatcaatgag attggaaaag cgtacaataa tactgcaacc agcatgtaaa aaatcatcgt
    19681 ccagctcgtt gctgctacat ctggaatatt tcgtacactt gctacaaggg cgcaggtcat
    19741 tccgataaag gtcatggtcg tgatttgctt tgatgaacca gtatctttca tctttattcc
    19801 ttctttcaat agcgacatat gtttttgttt tcttttattt ttgttcaaga acagaaatga
    19861 caaggtttca tcgattcagt acaaaaccca gtatcaccga aacatccatt gaacgatctt
    19921 agtgaactgg gtttgataca tgaaacgaac gatccaactt atagaacgtt ttataaaaaa
    19981 gaatatcttg gcgaggaata tatgaaaaac agggggaaat acaattgttt tgatgaagca
    20041 aataattgtc tgttctccat atgtctcttg ttattctttc acacaaaaat cagttctatt
    20101 ggatgatttc gtttagtttc ttttccattg ctttggcgat tttttttgta taaaaattga
    20161 aaattcgatc atcattcaaa taaggagtca tgatagccgc acgtagcaga gtgattttct
    20221 gttctttttt ccattcttct ttcgttagac ccattctttc aacaaaacga atcggagaat
    20281 caccgtaatc ttcttgtgta aatgtagtat gagaagtaat aaaccgttca ccataaacat
    20341 cgccatccat ataagaagac cgatcgaaca ttttttcatt taattcattg atagcgttca
    20401 aatctgtaca gccttgttct ttgaataccc aattgaccat attaaaatca ggatgattca
    20461 atggatagac ttcgattgtt tttcctttaa cagtaaaagt cagatgatcg agaaattctc
    20521 tgaatctttg agctgcctcg attgaagccc caatcaattg accatatccg gtcacattca
    20581 atggcaatac acgatgtgct gtccagactg cggcggcggt tgctccagct tttgatcctt
    20641 ccaaaatata agctcctaac atatctggag cctttactga tttttcaaac acatatggcg
    20701 cgaaataaga aatgatattt ctcatatttt tatgtttgat tacgattcct ccagcagcat
    20761 aagggacata gcccatctta tgaggatcta tcgtgacaga atccgcttct gaaatagctc
    20821 tgaacccttc atatacctct ttgtcaatcg ttacgcagtg gtggaaaaca tgatgttctt
    20881 caaagaattc agctaaggaa gcatacggca caaattctcc tgcttcgttt agaaacagtg
    20941 aacgcgcata gccgccatat gcggcatcaa catgtaaata gaaatagatc ccttcatctt
    21001 ttaacctttc tcttaattgg acgattttat ctacactgtc tacttgacct tcttctgtcg
    21061 ttcctacgac agcaaccaca cctagaattg ggattttttg tccagctaat tcacgaattg
    21121 ttttttccaa agcattaata tccattcgat aatcctcttg aactgggata gcaaccatct
    21181 gatctaagcc gactccgcaa atatctaacg ccttcatcca agaatagtgt tttgtttgag
    21241 ggaccagcca tttccctaat ctttggatat tttttccgct acgggaagaa gctgctttga
    21301 cctcatccat ttcttcgtcc gtaaagcttt cggtcatttc caaaatttct tcaacagata
    21361 aattcaaaag ttcccactca gacatttttt tcactttttc aggcagcact tccttgacag
    21421 ctaaaggaat cgatttgatg caacgggcgt accataatcc ttcaagatta gcaatagaac
    21481 catctgctgc aatgtgtccc catccgtctg tcatattgaa taatgaagca aaatcctgtc
    21541 ctacttctgc ttccatttgc gaagttgcca tagaagattc caatgcaaca ttatttgggt
    21601 tccacagcat cgcatagttg taagctaata aagcaggcat caatgtttca gcattcattt
    21661 gtccccagta acggcctgct gaatgccatg ggatcgatcc tgcgcgtaat cgctgactga
    21721 cttcatccaa gacctctatc atatggtcgc gggtggctag ataatctgga gtgagcttat
    21781 ctccttcgct aattgcaggc atatctgaag gaatataatt ttctctccat ccaagatgtt
    21841 catctaccat cttgttcaac aacattttat aaactggtcc attttcagct ttgtcgccta
    21901 taaagacggc cttgatatcc atatctttca tttttcttcc ctccatattt tgttggtcaa
    21961 tgtcttgcag atacatgtgt ttatctgtaa cacctcttca ctatagcgcc aaaaatgaga
    22021 attttttttt tataacatca aatccacgta gatttttaca aacaaaaata aataaagtcg
    22081 gatttttctg ataattcaac gcaaaaaagc gaacaatttt tcaataaatc agtcttctaa
    22141 aatagataac ctaaaaaaag cgaacaaaca aataaaatga ctgtccttca ttcactaaac
    22201 atatttttat ttttttatat tgttaaaaat tcgcaattta ttttttaata gaattatctg
    22261 ttttccaaat tttataacat aaaaaacctt ttttaacggc atttatatgc tttttcagaa
    22321 attaactgat gatttcaaaa agaaaacatg tcataatatt tcccatattg tgcaaaaaaa
    22381 tacaaggcgg aggctctact atgattgaag aatatattga gaaaaatatt ttaagacagc
    22441 tgtttttatg tgggcaattc tatgtaaata aagaagtcaa tctagaaaag ctctccaatc
    22501 tcctgcatgt ttgcaaaacc actttactca atgatatcaa taacataaaa aaagaatttg
    22561 aagaacagat cgcttataca catcgggaaa aggaccgtta cactttatat ttttcagaac
    22621 atatacctcg atgcaagatc atgcagcaat tgtctcaaaa ttcgttgttt ttaaaaactt
    22681 gtctgcttta tttggaagaa gatgaacctg attatcttca attaactgaa tgtgaattca
    22741 tctctgtcag caaagcttat tcattaaaaa aacaagtatt agcttatttt aatgactgcg
    22801 ggatcgaaat cgaccgctac tcccctcgat tcactgaaat ggaaagacgt ctgctgcttc
    22861 ttaatgtgtc gtatcgttta ggcggtttta attcttggga gctacctgaa tcgttttttg
    22921 aacgtgccga tcgtttcatt gaatccgtaa cggaaaatag tggcagattt tatgataaag
    22981 aaaataaaga aatcttatcg attggttttg ctatcagttt cctacgccag caagtgtgtg
    23041 ctgtgacaat agattcaaaa tttatcgaag aaatcaaaaa acgtcctatt tacaattatg
    23101 tcgaatcagc atgggaaaac actgatttcc aaacctatta taaaaaagaa gaattcgcct
    23161 ttattttgac actattcaat ctttgtaatt atggtttcca ttcttatcag ttgattgcgg
    23221 aagactttca acagctacac caagtgttta ttgacaatac tcccgaaata aaagaactag
    23281 ttgctacctt tgaatctcac ttcaatcaag aattattcgg aaaccaaccg ttcgaacgtg
    23341 cgctgattca tctgatgcgt tcggcttggg ataactatca attattcatg ccagaaaaat
    23401 tttatctttt aaatgaggaa cagacaaatt tatataaaga agtccagaca attttttcca
    23461 gctggtccag ccagttacct tatgatctac ggttgaatcc taattgcatg cgggcttttg
    23521 tgatcgaatt gagcggaatt ctccgtctga caaaagaaca tctgactatc tatatcgtca
    23581 caaattcgga tgttcattat ctgatttatc gggaagcctt ggaagctgta actactttcg
    23641 attttcaagt tgcaccaaca atttattcaa gtatatctga tataaagaag tatgcgcagc
    23701 agtcctcaaa ccgggtatta tgtgaacgta cattatatac accagatgcg gtccaatatg
    23761 aaaatattat tcctatttcg atcaatacga tcgatcgagc tatcatttca gctgtgcaaa
    23821 acaaataagg ggctgtgaca aaaatcatgt cacagcctct tatttgttga agatgcccat
    23881 ttctgtaaca atctcttgat acacgtcagg ctcacaacct cacttatttt ggacatctac
    23941 gggtccgatc tctttattta aaaaataaga aatgatggtt cggataacta ccaacgccgc
    24001 tagttggaaa atatcctgca aggtaggttt gataatcgat tcgatgatcc cagctgaaac
    24061 aagaatttcc aaacttagaa gtatatagcc tcccaacatc tttttaatag cattgttctc
    24121 gctgactcgg tcgctttgca cttttgtatg gacaaataaa tatttcaaaa aatccttgat
    24181 tgatagacaa acaccccaaa ccaataaaca gatagcaaag atatcaagtc ccagtacaag
    24241 tttttcgaaa tatggaagaa gaaactgcat caactctgtc gtcatcctac ctctcctctt
    24301 ctttgttttt ctttgtcttc tatttcctga ttcaagaaat aagaaatgac tgttcgaatg
    24361 ataataataa gtgcaagctt caaaatatct gtccaagtcg gcttgataac tgactcgatg
    24421 atatcagcaa cgactagtac ttccaagctt aacaaaatat aacttgctag cagttttctt
    24481 gtatctgcat tacgagtatt acgttctgca tagtttttct tgacatgaga ccaatcaaaa
    24541 agtttgaata gaacaagaac gatcccgaca attaagatac cgatagaaca aatatttaaa
    24601 atagcaatga tccattctag aaattcaatt aaatttgagc gaaacatata cgctacctac
    24661 tttcttttaa tttgttccta ttcttttatt gtaaaagaaa gttaccttgt tcgtctattt
    24721 aaaagttgtt ctaatgataa cggttcgctt aaatttttct ttttcactct cccatttttc
    24781 tccctccatc cataaatcgt ggaagtatcc aaaacaacgt agcaagttaa aataaggctt
    24841 agtccgtcat cagcttttca aagaagcact gtctcggtgc tttttttgta cccaaatttc
    24901 taaaaataga accatacacc tcctcaaatc ttttcttctt acctacccta tttttattta
    24961 caggaaaatc agacacttct tcttttttgt caaaatttcc tttttcttct cgtcctatca
    25021 tttggcattc taataaaaac gaactatcct tggatataga aagagaaaac aaggaggcgt
    25081 ttttcatgag taaaaaatta atcaaagtcg gcatcggttt aggtttgctt gcccttggcg
    25141 ctgcatatct aggaaaaaag acaggactat tcgaagatga cagtcattta tatgatgaat
    25201 tcgaatcaat ctagcatcct atttagttga atcactacgg acatacttgg aagacattgg
    25261 aggaatcaca atgagaaaaa gtaaagcaat aatgattggt gccggtattg ccaatatggc
    25321 agcggctgtt tatttgattc aagaaggcaa atggaaaggg gatcagatca ccttttattc
    25381 attggatgat cacggttcaa atgatggtgc accgaccgac actgtagtgg atgaatattg
    25441 gaataaaaac catccattag aaaaccaaaa aggctatatt gctcgaggcg gtcgcatgct
    25501 taattaccgt acctatgtag atttgatgga tctgttaagt cgaatacctt ctgttactga
    25561 gccagggatg actgctgaag aagatacgag agattttgat gcaaaacacc gaacctttga
    25621 caaagcacgt ttgatggaag gtggcaaagg aatcattgat ggcggaaaac tgggattcaa
    25681 taataaagac cgtattcttc tgactaaact gattgctatg cctgattcag aagaagaaaa
    25741 actagacaat atcactatcg aagaatattt caaagatgat ccacactttt ttgaaacaaa
    25801 cttctggttt atgtgggaaa caacatttgc tttcagaaca cgtagttctg ctcaagaatt
    25861 gcgtcgctat atgcatcaaa tgatttatga attcactcaa atcgaacatc tagtgggcgt
    25921 caatcgaaca cgttacaatc aatacgaaag tattatgctt cctttgatcg agtacttgaa
    25981 agaacaagga tgcaaaatca ttttgaaccg ccgtgtcact gactgggaat tcaaagaaac
    26041 accgatgcaa gatgaaatca ctgtgactgg tttgaaaatt accaatacac tgactcaaga
    26101 agaagaatac gttgtggtcg atgaagatac agcagttatt ttcactaatg ggtccattac
    26161 agattcagct actttaggag attacgaaac accagctgta gaaaatatgg attatggtgc
    26221 agcttctagt ctctggaaaa aagccagtga acgtttctac aacttgggaa atcctgacaa
    26281 attctttgct gatcgagatg caagcgaatg ggtcagcttt accctgacga cgaaaaacca
    26341 tttgttgttg aatgaaatca cccgtatcac gactcaagaa ccaggaaatg ccttgaattc
    26401 atttatttca accacaccga tcacgccgtt aggccaaaaa gatgtcaata tgtctattgt
    26461 cgtgcatcat caaccgcatt ttacgtctca gaaaccaaat gaaacagtta tttggggtta
    26521 tttcctttat ccaagacgac gtggcgaatt tgtggataaa cagtatatta aaatgaatgg
    26581 aaaagaaatg ctagaagaac tgattggaca actttcaaaa gtagatcctg gaccagtgaa
    26641 tattcgtgaa aaagaaacag aaattttcga cagcgtaata aataatatcc ctgtctatat
    26701 gccttatgct tccgcgttgt tcaataatcg tgccaaaagc gatcgaccaa aagtcattcc
    26761 taaacattcg accaatctgg cctttacagg agaatttgtc gagcagcctt atcagatgat
    26821 ctttactgaa caaagtgctg tacgttctgg agaaatcgct gctttccatt ttgcgggtat
    26881 tcctatgtcc cgtcttgtca aaacaccgcg ttacgacaaa gatattccta ctctagctcg
    26941 tgccgcaaaa aaaatgtttg aataatcaag aggttgtgaa aaagctgccc tgcatcaagt
    27001 aataagaaca accaggctaa aacagcttct tatgtttttg atttggttgg acttattacc
    27061 gcagatgcaa gcttttgaac accgtttacc aagaggttat gaaaaagctg ccctgcatca
    27121 agtaataaga gcagccaagc taaaacagct tcttatgttt ttgacttggt tagacttatt
    27181 accgcagatg caagcttttg aacaccgttt accaagaggt tatgaaaaag gcgtttagtt
    27241 tcgagtatga agaaaaattt tgtgaaaatg gttcctcacg tttttgcata aatttgactt
    27301 aataccgaga aactgatccc ttctgtcata acctcttttt atttatgtct gtttcttttt
    27361 gaaaacaact attttttcaa cgtttactct cttgccaatc caacatcatc taacggatac
    27421 aactatttca atgattacca ttacactaaa aaatcggaaa cacacctctc cgaacacaaa
    27481 tacataagac tatagaatgt ttcatttaag aaaaaaagcg tttcattatc aaaaaggtaa
    27541 gatctccttt aaaatgaaat tatctgtagt actaataatg cagacaattt tatgaatctg
    27601 aaaggagcat ctccaatgaa agcagtcact tggcaaggaa aacagaaaat ggaaatcaga
    27661 aacgtagatg atccaaaaat attagaacca acagatgcaa tcatccgtat cacagcaaca
    27721 gccatttgcg gctccgatct gcatctttac catcatggtg aagcagtttt ggaaaaaggg
    27781 tatgttgtag gacacgaacc aatgggaatc gttgaagaag tgggatctca agttcaaaca
    27841 ttgaaaaaag ggcaacgtgt ggttatccct tttaatatta gttgtggtca ttgtcatttc
    27901 tgcctgaatc acatggaaag ccaatgtgat gaatcaaatc cagatcagtt atatggcggg
    27961 cttttcggat ttggcaagtt gaacggtaat cattggggtg gtcaagcgga atatctaaga
    28021 gttccttatg cagactctac ttcatttgtt gtaccagata atgatcttcc tgatgaaaaa
    28081 gttttatttc tatcagatat tttgcccaca gcttattgga gcgttgaaaa cgcaggagta
    28141 aaaaaaggcg atacagttgt gattctaggt tctggtccag tcggtctatt cgctcaaaaa
    28201 ttcgctgtta tggctggagc agagcgcgta atcgcagtag atccagtaca gcaccgtcta
    28261 gataaagcgg ctaattacaa tggggttgaa acttatttct tagaagacac ggcacaagct
    28321 ggaacagaat tgtatgaact ggctaaaggt ggcgcagatg tcattatcga ttgtgtagga
    28381 atggacggat tagagcctgt caaagaaaaa gcgaaaaatt tagtaagctt acaaagcggc
    28441 acaatctctc cattacagat ggcatcgcaa gctgtcaaaa aatttggaac agtccaagta
    28501 actggtgttt atatgactcc agcttcttct tacccgctgc aagaattttt tatgcgggat
    28561 atcgctgtca agcatggaca ggcaccaatt atccatctaa tgccaaaaat ttacgcaatg
    28621 attgcagaag gactattcga tcctacacaa atcattactc attcgctgcc actatcagaa
    28681 gcagcacaag catatgagat ctttgacaaa aaagaagata aaaatataaa agttgttttg
    28741 aaaccttgaa ttcagtaaaa aatacaggtg attttcttta tgcccaaaat caaacgaaat
    28801 acccatgaga tcgaaatagg aaaagaagaa gatatcgaaa tacgaggacg tcgcttccgc
    28861 ttctatttcc aaaatcgtta cacattgatc tcactggctg tcgatttatt aaccgggatt
    28921 ttctatattt ttggaagcct tgcttcctta actaaaatcc ccgatcgata tggcacctat
    28981 ttttatttgg ctggtgctat tttcttgacg attcgtccta ttctcagaat cgtaaggaac
    29041 atttttattt acgacgaaaa aaaagcaatg aagagaacaa gcaaaaaaaa agaggcatga
    29101 gaatttttca aatctcatgc ctcttttttc accctatcgt atcactttga tacagacgag
    29161 cggtcgtccc actttcttcc ttgaccattg tcaaaaatgt ccactcgtat cgttcataaa
    29221 acgtggtatg atctgtaatt aagtaaagtt tttcgtaccc aagttgatgc atttcttttc
    29281 tgatttccgc taatatccgc ttggctatcc cctgcttacg cttttccggt tcaacaaaca
    29341 gggcacaaag atttggtgtc agatcagggc ggttatggaa gtcgttttca atgacgcccg
    29401 ctcctccgac gatggtttct ttttcatcta agaccacata ccattgagga atactttcct
    29461 tattagcgat tccagtacga atactttcct catacacaac agcaggaact ccccattttg
    29521 atgaaaacca attcgcgctt tgttttacta attctgggtg ttctcttaat ttgatccagt
    29581 tatatgtaac caatgtttaa acctccgact aaaataaaac gtttacaaaa attatacaag
    29641 tctttatgaa aattagcaat gacaacaata gtgatgtaaa aaaaaaaaac ggaaaacaat
    29701 cacttgttct ccgttttttg attaatcaag catctaaagc ttcttttctt ctttcttgga
    29761 tcgtagctac cttgacgaac ggtagataga tcaaaataga aagaaccaaa ttgacagcgg
    29821 ctagtacacc acctgcaata ctttttgttg ccaatacacc gccaataatt ggtggagtta
    29881 cccatggagg cataacggta gctgctggca ctaaacctgt agaagtcgca aagtaagcaa
    29941 cactaacta
//
LOCUS       DAZUKC010000031        29141 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000031 DAZUKC010000000
VERSION     DAZUKC010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.032"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(50..1345)
                     /gene="purB"
                     /locus_tag="ACG80T_001301"
     CDS             complement(50..1345)
                     /gene="purB"
                     /locus_tag="ACG80T_001301"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFS4840962.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1358..2482)
                     /gene="purK"
                     /locus_tag="ACG80T_001302"
     CDS             complement(1358..2482)
                     /gene="purK"
                     /locus_tag="ACG80T_001302"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFS4840963.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2475..2963)
                     /gene="purE"
                     /locus_tag="ACG80T_001303"
     CDS             complement(2475..2963)
                     /gene="purE"
                     /locus_tag="ACG80T_001303"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFS4840964.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3379..4686)
                     /locus_tag="ACG80T_001304"
     CDS             complement(3379..4686)
                     /locus_tag="ACG80T_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFS4840965.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4689..5270)
                     /locus_tag="ACG80T_001305"
     CDS             complement(4689..5270)
                     /locus_tag="ACG80T_001305"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFS4840966.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5386..5483)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5842..7134)
                     /locus_tag="ACG80T_001306"
     CDS             complement(5842..7134)
                     /locus_tag="ACG80T_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4840967.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7131..7754)
                     /locus_tag="ACG80T_001307"
     CDS             complement(7131..7754)
                     /locus_tag="ACG80T_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840968.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7757..8593)
                     /locus_tag="ACG80T_001308"
     CDS             complement(7757..8593)
                     /locus_tag="ACG80T_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4840969.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8610..9497)
                     /locus_tag="ACG80T_001309"
     CDS             complement(8610..9497)
                     /locus_tag="ACG80T_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4840970.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9513..10796)
                     /locus_tag="ACG80T_001310"
     CDS             complement(9513..10796)
                     /locus_tag="ACG80T_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4840971.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11479..12162)
                     /locus_tag="ACG80T_001311"
     CDS             complement(11479..12162)
                     /locus_tag="ACG80T_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFS4840972.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12180..12797)
                     /locus_tag="ACG80T_001312"
     CDS             complement(12180..12797)
                     /locus_tag="ACG80T_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFS4840973.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12807..13631)
                     /locus_tag="ACG80T_001313"
     CDS             complement(12807..13631)
                     /locus_tag="ACG80T_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFS4840974.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13624..14184)
                     /locus_tag="ACG80T_001314"
     CDS             complement(13624..14184)
                     /locus_tag="ACG80T_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840975.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14358..15314
                     /locus_tag="ACG80T_001315"
     CDS             14358..15314
                     /locus_tag="ACG80T_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4840976.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15619..16575)
                     /locus_tag="ACG80T_001316"
     CDS             complement(15619..16575)
                     /locus_tag="ACG80T_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFS4840977.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16679..17281
                     /locus_tag="ACG80T_001317"
     CDS             16679..17281
                     /locus_tag="ACG80T_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS4840978.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17369..18868)
                     /locus_tag="ACG80T_001318"
     CDS             complement(17369..18868)
                     /locus_tag="ACG80T_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFS4840979.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18884..19408)
                     /locus_tag="ACG80T_001319"
     CDS             complement(18884..19408)
                     /locus_tag="ACG80T_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFS4840980.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19706..20455
                     /locus_tag="ACG80T_001320"
     CDS             19706..20455
                     /locus_tag="ACG80T_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFS4840981.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20446..21231
                     /locus_tag="ACG80T_001321"
     CDS             20446..21231
                     /locus_tag="ACG80T_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4840982.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21200..21916
                     /locus_tag="ACG80T_001322"
     CDS             21200..21916
                     /locus_tag="ACG80T_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS4840983.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22018..23037)
                     /locus_tag="ACG80T_001323"
     CDS             complement(22018..23037)
                     /locus_tag="ACG80T_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFS4840984.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23056..24057)
                     /locus_tag="ACG80T_001324"
     CDS             complement(23056..24057)
                     /locus_tag="ACG80T_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFS4840985.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
     gene            complement(24335..25483)
                     /gene="nagA"
                     /locus_tag="ACG80T_001325"
     CDS             complement(24335..25483)
                     /gene="nagA"
                     /locus_tag="ACG80T_001325"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFS4840986.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(25559..26395)
                     /locus_tag="ACG80T_001326"
     CDS             complement(25559..26395)
                     /locus_tag="ACG80T_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4840987.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(26579..27796)
                     /locus_tag="ACG80T_001327"
     CDS             complement(26579..27796)
                     /locus_tag="ACG80T_001327"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFS4840988.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(27945..28106)
                     /locus_tag="ACG80T_001328"
     CDS             complement(27945..28106)
                     /locus_tag="ACG80T_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840989.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            28179..28262
                     /locus_tag="ACG80T_001329"
     tRNA            28179..28262
                     /locus_tag="ACG80T_001329"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28213..28215,aa:Leu,seq:gag)
     gene            complement(28502..>29141)
                     /locus_tag="ACG80T_001330"
     CDS             complement(28502..>29141)
                     /locus_tag="ACG80T_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840990.1"
                     /translation="TKKMLVKFTNIKKYRAFFLDMNILFLLLFFLIFLLTYRKCQNYY
                     VRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYP
                     GTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGM
                     RTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         8676 a         6235 c         5009 g         9221 t
ORIGIN      
        1 ggttattttt gctattctat tttaattgaa ttttgcaact cactcttctt tattccaaac
       61 ctactcgttt gaatatcacg tcaacgtttt tcaaatgatg atgataatca aaggcatcgt
      121 ctatttcttc tttagataat accgatgtga tggttggatc ttcttctaac agcgggcgga
      181 actgcacttg gttgtcccaa gcgtacgcag tttttggctg aacaagatca taagcttctt
      241 ctcttgtcat tcccttatcg atcaatttca gcataacgcg ctggctataa atcaaaccaa
      301 atgttgcaga catattccgc ttcatgtttt caggaaagac cgttagattt ttaacgatat
      361 tgccaaaacg atttaacatg taattcaata aaatcgtggt atcaggcaag atgattcgtt
      421 ctgctgaaga atgagaaata tctctttcat gccataatgt gacattttca taggcagtga
      481 ccatgtgtcc gcgcaccact ctggctagac cagccatatt ttctgaaccg atcggattcc
      541 ttttgtgcgg catagcagaa gaaccttttt gacctttcgc aaaaaattct tctacttcac
      601 gtgtttcgga tttttgcaat ccgcgtatct ctgttgcaaa acgttcaata cttgtagcaa
      661 taagcgccat cgctgagaaa tattcagcat gtagatcccg agggagaact tgagtagaaa
      721 tttcttgtgg acggatcccc aattgtttac atacatactc ttcaataaat ggcgggatat
      781 ttgcgaacgt tcccactgct cctgagattt ttcctgcttc tactcctttt gccgcatgtt
      841 caaatcgttc gatgttccgt ttcatttctg aataccatgt ggcaagtttc aatccaaaag
      901 tagttggttc agcatggaca ccgtgtgtcc gacccatcat cactgtatat ttgtgttctt
      961 ttgctttttc gccaatgatt tccgtaaaac gtttcaagtc ttcccgtaaa atatcatttg
     1021 cttgcttgat gagatagcca taagcagtat ctacgacatc cgtacttgtt agaccataat
     1081 ggacccattt tctttcttct cccaatgttt cagaaaccgc tcgtgtaaac gcaacgacat
     1141 catggcgtgt taatttttcg atttccaaga tccgatcgat atcaaatgat gcatgctcac
     1201 gaattttttt cacatcttct tgcgggattt caccaagttc tgcccaagct tcatctgcta
     1261 aaatctcgac ttcgagccac gcacggtatt tgttctcttc cgtccagata ttccccattt
     1321 cagggcgtgt ataacgttct aacatgtttt ttctcctcta atcccaaata ttcgtttgtt
     1381 tgatttcttc cagcgtttgc tggatgtctt ctgtcaagat agtgatatgt cccatcttgc
     1441 gacctttttt tgcttgtttt tttccataga aatggaattg ccagtctggc ttattctcaa
     1501 tcaagcgata gctttccagc agttgttctc ccaaaacatt gaccattact gcattgctta
     1561 tcaaacgtac tttttctcct aatggccaac cacaaatacc tcgaatatgg gcatcgaact
     1621 gactcataga acaagcttca atagaataat gtccagaatt gtgaggacga ggagctaatt
     1681 cattcacata tagcccgcct gtttttgtta aaaacatttc tacccctaaa acacctgaaa
     1741 ggctcactgc ttctgcaatc acacgggcaa tacgctcagc ctcctcgatc acatcctgat
     1801 caatcgcggc tggagcaatc gtttcatgaa gaatattgtt gtggtggatg ttttccacga
     1861 ccggaaaagt cgtgaaatct ccttgaccat ttcctgccac catgatcgaa atctcttttt
     1921 caaacgggat ccatgcttct aaaacacagg ttccttctct taaaaggtcc atggcaggag
     1981 ctagatccgc ccgacttttc aaaacatact gccccttgcc atcatagccg cctcgagtgg
     2041 ttttcaaaac acaaggatag ccgatcccgt caatcgcatc ttggatatcc gtcggactga
     2101 cgatcgttgc ataaggagca atcacgatgt tattcgtttc caaaaaagat ttttctaaca
     2161 ggcggtcttg cgtaatcgca agcaagtctg tcccttgagg aataaatgac atgggtaaaa
     2221 tcgcatttaa cgcctctaca ctgacgtttt cgaattcata ggtaatgaca tctgttcttc
     2281 tggcaagctc tttcagcgca aaggtgtcat catagacact ttcaatatgc caatctgcaa
     2341 cttgtgcggc tggacaatcg ctcacaggat ccagcacacc tactttaaag cccatttctt
     2401 ttgcactaat cgccatcata cgacctaatt gaccgccacc gacgatcccg atcgttgaac
     2461 caggcaacaa aggtttaacc aagttgatca ctactttcta taacagtttt agccaacatt
     2521 gaacgtcttt cagccaattt agcctccaat tctaaatcat acatggaaag catctgtatc
     2581 gcaagtagtc cagcattaat tgctcccgct tttccgattg ctgtagtagc tacaggtact
     2641 cctccaggca tttggacaat cgaaagaagc gaatccaaac catttaatgt tcgtgattgg
     2701 accggtacac cgataaccgg caaagttgtt tttgatgcga ccatacccgg cagatgcgcg
     2761 gctccccctg ctcctgcaat aatgattttg attccttttt ttcgagcgtt ttgtgcatat
     2821 tcaaacatca aatctggggt tcgatgagca gaaacgacct ttttttcata agacacaccg
     2881 aattcatcca atatttgaca tgcttctttc attgtttccc aatctgaagt actccccata
     2941 atcacagaga tttttgctgt cattgttttt cctcccagtt tttctacatt catattttaa
     3001 caatcacttt ttttgatgtc aaagaaattt cgaatgttaa caaagaaaaa cattttattg
     3061 ttcgtgttta ttaaagataa aaaaaaggaa tgtgacaaaa gttaggtcac attccttttt
     3121 atggataaac ggtgttctcc tgccaactcc tcggtcacaa ggaacaatat tcaacaaaca
     3181 tgagaagctt ttttttgaaa ttgttccttg tgactcggag atgaacggca ggctcacgac
     3241 ctcttccttc aacggtgttc aatcagctga ccctcggtat tagctcaaac aaacggcaaa
     3301 atacgaggaa ctatttttcc gtttgtttct ctaacaatca ggtcaaaacg ctgatttcac
     3361 aacctttctt ttgctctatt acttcttgaa ggcttcttcc aatatttctt catctttttt
     3421 caatccatta aagatcaaat tcaatatgat agccgtaatg ctactcatta cgatgccatt
     3481 tccagtgaac atttgcaatg tttgcggcat ttgactgaat aatgtcggcg taacattgaa
     3541 acccagccca aatccaacag aaacagcaac aatcaataga tttttatcat tgctgaaatc
     3601 aacttccaac agcatacgga ttccttggac tgccaccatc ccgaacatga ctaacattcc
     3661 tccaccaagt accggctcag ggatgatttg tgctaacgca ccgattttag ggaataatcc
     3721 taaaacaatc aagaagaatg cagagaaata gatgggtttt cttgttttga ttcccgaaag
     3781 ttgaaccaat ccgacatttt gagaaaagcc agtataaggg aatgtattga aaataccgcc
     3841 taagataacg gcaagtcctt ctgcacgata tccctttttc aattcttctt ctccaacatt
     3901 tttcccagta atgtcaccaa gtgcaaaata tacacctgtt gattctacca tgctgaccat
     3961 ggaaatgatg atcattagta aaatcgacca tacgtcgaaa gttggtttac caaaataaaa
     4021 gggttgtgga acatggaaca acggcgcatc tccaatcgcg ctcagatcaa tcattcccat
     4081 gaaagcagct aagatgcttc ctccgactaa accaatcaat accgcgattg aacgaatgaa
     4141 cccttttgcc agcatttgca cgcctacaat taggagaatc gtcacaaatg caagtagcag
     4201 atttgttttg tcaccaaaat cagctgctgt tgcattacct ccacccattt tctcgattgc
     4261 tacaggaatc aaagtcaaac caataacggt gataaccgtt cctgttacta taggagggaa
     4321 tagtttttta attttagaaa agacaccaga aactaacaca acaaagatcc cagaagcgat
     4381 aatagatccg tagatagctc ctactccttc attggttccg atcaagatca gtggagctac
     4441 tgcttggatc gcacatccta aaacgacagg caagccaatt ccgaaaaatc ggttaactgt
     4501 caattgcaat aatgtcgcta atccacacat aaagatatca attgaaatca aataagtcat
     4561 ctgttcttga ttaaagccta gtcctgtacc gatcaaaaga ggcaccgcta ctgctcctgc
     4621 atacatcgct agcagatgct gcagccctaa aaccgcagct tttccatttt gtgtttcttt
     4681 ttccacgatt atgcgtcctc ctcaagaaac tcaacttgtc cattcgtcaa ggaagcaata
     4741 cgcgcaagag aaacgactct caatcccatt tcttcaagta actgacgtcc atcttggaaa
     4801 gacttttcga tgacgatccc gattccttct acttgcgctc cagcttgctg gcataattcg
     4861 atcaatcctt ttgccgcttg tccgtttgcc aagaaatcgt cgataatcaa tactttgtct
     4921 tctgaagata aaaatttacg agaaatagaa atcgtacttg tcacttgttt cgtaaaagaa
     4981 taaacagaag ctgtcaataa ttcttcatcc atcgtcaaac tttttgcttt tctagcgaag
     5041 atcattggaa catctaattc tcttgcagca aataaagctg gtgcgatccc agaagcttca
     5101 atggtcacaa ctttcgtgat tccttgttct ttaaatactt ctgcaaaccg ttttcccatt
     5161 tgttccatca atacagggtc tacttggtga gtgacgaagc tgtcgacttt taatacgccc
     5221 tcgcctaata tacgtccgtc tttttgaata cgttcaacta attctttcac tttatttgct
     5281 cctttatcat ttattaatac cgaaataacc gcttttgtgc aaaaacctac tatgtttctg
     5341 ttaaagaaaa acaccctcca ctttatcata agaaaagaag ggtgaaatag ccaatctttt
     5401 acttatagtc cagcatttac ggtactggct agaaactgtc atccatatta tgacgatata
     5461 taagtaggta tgtaaaacta ataatagtgt aaattcattt tcttgcaact gttttgcacg
     5521 tgaaggcagt ttgccatatt tgaataaaaa atgttgtttc aaatattgaa aacagtctaa
     5581 cactactttc tgaaactgac aacgtggttt acttattttt ttgtaaaatc taatgatttt
     5641 taatgcatta ttcctttttg ttcgggttta aatagttatt tttattttct cctatctaaa
     5701 aaagcagccc acataaaaag aaggcatgac aaaaagcatg tcatagcccc tttttccgaa
     5761 taaatagtgt tcaaaagaca gcctatcggc aataaggcaa gattttataa actacgggaa
     5821 gtaatttccg taaatctatt cttattcttt taagctgaca agctcgtcaa tcactactgc
     5881 ttttcctgaa gcaatagaat gatttgctgc gattcctgtc aaaatcgaca aaataccatc
     5941 tacgtgagtt gctgcccgat tccacggatc ttcttctttt tcttcaccaa atagatcatc
     6001 taataaacgc ggatctcctc cgccatgtcc gcccgcttct tgttcgacga ttacctcata
     6061 tggctcaccg aacatcggat agacatggat ggactgaaga acggctgctc cttcatcctt
     6121 tttatctcca cctgcattga tgtaagactt ttcgatcact ttcatttcga tccgtccttt
     6181 gctgccattg aaagaaacat tgaatccttc ccacggcata tacgcgttta gcgaatacgt
     6241 caaaatcgca ccagtttcat atttgaccgt tacgcctagt gtatcttcaa tagagatatc
     6301 atcggaaaat acactgcggt cacgaatata tccactgtct ttttctgctt ctaaatacat
     6361 ttctcgcaat tcctcattgg ctgacaggtc tatagcaaat ggatcagctt tagctagttc
     6421 gcttccatag catcgactgt aaaatatttc tactccgcgt ttctccgcat tatgcatccc
     6481 ataaaagttt aatccaccga atgcaaatac cttttgggga cggctgttca accaaaaatt
     6541 cactagatca aaatgatggg tagatttatg aacaagaagc ccgccgctat tgtgcttatt
     6601 tcgatgccat cttctgaaat aatctgcccc gtgttgcgta tttaacagcc attcgaagtg
     6661 gatagaaaac acatcaccaa tcacatcact ttctatcagt tcctttatct tcgtgtgata
     6721 cggtgcataa cgataattaa aagtgactcg caggcttctt cccgtctgtt tttggaccgt
     6781 tattatttct tgagcttttt cctcatcgat agtcagaggt ttttcgcaaa taacatcata
     6841 tccttttttc attgcacgaa tgatataatc atgatgcgtt cgatctactg acgtaataat
     6901 gacttcagcc ggccgtacgt catccagtag ttgctcaaat tgtgatggca tacatgtctt
     6961 tacttttttc tgtccatact tgttcacaag tatccgattt gcataatcca tccttgtttg
     7021 agatacatca caaaaaccga ctagttcaca agtttgatga tacgtagagg caattgcctc
     7081 ataaaaaaaa cgcgctctcc caccaagtcc gacttgtaca tattttttct tcatttaaag
     7141 tcattctcct tttcttttaa gcaaaatact gtgacaaatg gcagccctgg atacagtaat
     7201 gcagtgagta aaaaatcatg ctgtatacac cagaacagtc catacaaaag gcataaatac
     7261 cacagaaacc ccttgacgtt ttttgacaac agccagatat tccttttgat caaataaaca
     7321 agactatctg ctttatcttc tttgcagata tcaattagcg tactaaaaaa actatgggat
     7381 acaaaaagca acagacacaa tagaaaagga atcagatacc aagctccaat ctttcccact
     7441 aataaatacc aatcaactgc aaaaataatg acaaacagct gaaacaacgt aatgatgaaa
     7501 cacatccgtt tgatttttcc tttcctccaa catacctttt tttctgccaa gtaatggaca
     7561 gatagataat aagaaggata catcatcaaa aaataaatca gatagacaac acgagcagac
     7621 agcactaatc ctaaagtgaa caaacatcct agaactaaag cgaaaagagc attactgata
     7681 agactggtta aaaaaatccg acagacatag tagttgagct tgtaaagaaa catctcttct
     7741 attggctttt tcatattcag cccttcagac cgcttgtact gatcccctgg acaagatatt
     7801 tattgaagaa taagaaaatc agaaaaaccg gtagcagcga caacgtagac atcgcaaaca
     7861 tactcgcgta agtcgtacta gatccagcgt ctgcaaataa tttgatggca atcgataccg
     7921 tgtaagatct cggcttgttt aaataaatca atggtcccat gaagtcgtcc catttccaat
     7981 aaaactggat gatgatcgtc gtgatgatcg aaggtttcag caaaggaaaa ataattttgg
     8041 taaagacact atacttgctg catccgtcaa taatcgctga ttcatctaac tctttgggga
     8101 tgctgaccat gaattgcatg atctggtaaa tgaaaaatgc ttgtccaaaa aaatgtggta
     8161 aaatcagcgg cacatacgtc ccaaccaaat tcagccgatt gtaaataata aactgcggaa
     8221 tcaaaatcac ttgtgcagga atcatcatcg ttactaacat gacgacgaac cagaatcttt
     8281 ttcctttgaa atcgattctc gataatgcgt aagcaataca agtcgagctc agaactgtcc
     8341 ctatcgtcga aactgcagag atgacaaacg aattcctaaa gaaagtaaaa aatgaaattc
     8401 cagcaaaccc cgcccatcct tttgcgtaat tttcccattg aaaagtctct ggaatcaaat
     8461 tcagcgaacc actcataatc tcttgatccg ttttaaaaga acctgcgatc atccataaga
     8521 ccggataaat catgactaga cctaatagca aaataaaacc gtattgaaac catttcgtta
     8581 ctgtattatt catttcttcc tcctctcatt cacacaacga cccatttttt tgatgatttg
     8641 aaaatcagaa aggtgacagc tcccataatc aacagcatga tccacgaaat agcactagca
     8701 taccccatag aattaaaaga aaaagcttgg ttatacacat aaagagcata aaagttcgtg
     8761 gcatcattcg gaccaccttg ggtaatgata aatgcctgcg taaaagtcat aaacgccgcg
     8821 attgtctgca taaccaagtt atacaaaatg atcggcgaaa gaagaggtaa cgtgatcttg
     8881 taaaacgtct gccatttgtt cgctccatcg atctctgccg cttcataata cgagcctggg
     8941 atctctttga tacctgcagc aaagatgatc attgccgaac cgaattgcca agcagacatc
     9001 aaaacaagag ggatcatgac cagtttttga tcgctaaacc aggaaagcgc aggtgcgcct
     9061 aatcgttcca ggaaaatatt cactaacccg tttttagaaa acaactcccg ccaaactaga
     9121 gcgatagaaa tactgccacc gatcaaacta ggcacataat acaaagaacg aaagaaagtc
     9181 gttcctttga tattcctcgt taaaagataa gcaatcaata gaccaaagat cagtttcaca
     9241 ggtacagatg caaatacata gagaaaagtg actcggatgg agttcaaaaa acgggcatca
     9301 ctcagcatct ttatataatt atccaagccg atccatgaga ctggatttcc caaacgataa
     9361 tcggtgaacg aataataaag tgacaagatc atcggtatca ctgttatcaa tagaaatcct
     9421 aggatgaatg gagaagcaaa tacgtaaccg accacattgt cttgatccaa gaaccgtttt
     9481 agtttttctt ttttcatgcc ttcctcctgc tgctatccta gaatgtttga cgcaaactca
     9541 taaagactct ctgctgcttc ttctgctgtt acttcttcat aaatgacttt ttcaactaaa
     9601 agattgtatt ggtcttgaat ttccggattc aaagcactat caaagacatt cacttcgccg
     9661 ttgtccatat cggaaatcat ttctaaataa tcaaatgtcg cttgcaaagt tgcatcatct
     9721 gcacgagcga gctgttctcg aatattcgac atgattggta caccacgttc tccttttaaa
     9781 atttcattgg cttcttcact gttttgaaag aaatcaataa acttagcagc ttcttcttta
     9841 tgggctgagc tttttgccat actcagcatt tgcgaagaac gtaaagggat accagattct
     9901 ccatctgctt ttctgcgagg tggattaatc aactgaatct ccatatcttc aggagcccct
     9961 gacaatattt ctgtcaactg gttacttgat agaaacacca ttgccgcttc tctatcccta
    10021 accgcctgaa aagacagatc cgtcgtactt gcagctccac cacctggttc tggatgggcc
    10081 cctttgtcta ccagattttt acgcagctgg aaaaaatctg ttagatagtc tggattttca
    10141 aatcccaagc tgttagatag atcttcccga ttataaaaat tcaatccttt ctcaacttgt
    10201 ggtatttgaa taatacagcc agctataaaa tcttcgaatc ttgaaatagc atagattcct
    10261 aattcttcag tgatttgata gctgatttgt tcaaattcat cccatgtcca atttgaagct
    10321 ggttcaggga tatttaattg ttcaaatata tttttattgt aagcaatcgc aaacgaattc
    10381 accccgttag acagtccaac taattgcccg tcgtaagtcg tcgttgctaa ataggattca
    10441 tctgcattag aaatatcaat tatcccttgc tccacatatt catctaatgg ttcaatctga
    10501 ttgatgtatt gaggaaattg attgttcagt tggaaaatgt cccagatatc atctgatgct
    10561 gctagtgtac ctaattttgt gtaatatcct tcataatcat aaaattcata atcgatcgtc
    10621 acatggggat ttttttcttc ataaagctcg attactttta ctgttgcatc gtgtcggact
    10681 tgcgaacccc accaagccat gttcagcgtg atttttttgt catcttcttg cccacaagcc
    10741 gtcaagaaga ataacataac acttaaagct aagatgattc tcattctttt cttcattctc
    10801 tgcacctcta cttccattgt tatttttcaa tatactgtat ttttatacag aacatattta
    10861 ttaaacgatc tttttcaagg ggaataaaaa ccaagacttg ccttatcgtt ctgcctagga
    10921 tgaacaactc aatactttca agaaaaatta gatagtgctg attcttcttt catcgctaaa
    10981 aacacttctt gcttcaatga tgggaggata attacgcaaa gaaaaaagga tatcagacaa
    11041 ctgcccaata tccttgacat agctaaataa gcatgcttct tcgtgtgcgc tcttccctaa
    11101 aaaacagaaa aataactagt aattctgtat gaaattaggt acaatataca cgtcgatgca
    11161 caagcattga ctatggcaac ttgattggtc agttcccgct gacttttcaa gccccgacaa
    11221 tttagctgct actaaattgt cgggtgcctt ttttagtttt tattcgtttt tcattctata
    11281 gaaagcgctt caataaatca agacattttt ttgttagaat gctaataata acaaaaggtt
    11341 ttaactcttc ttatgtacat tacaggtaaa aaaacaaaaa aatttctctt ttttttgaat
    11401 tctgaacaaa tagtactgga gtacaaaaga cgaggaagag ccactatctg ctcttcctcg
    11461 tcttttgatt ctcacctgtt attcatgatt ctttttctca gtttcccgat atttttccat
    11521 tttacgacta aaaagctctc cgctacttgg tggcgtatat gtgatggcat ttgctcctgc
    11581 ttgaatggtc tcagcaatac tttcttccgt tggaccacct gtcgcaatga taggaagttc
    11641 aggatatttt ttacgaaaat ggcgtactgc ttctgcagtt tctttacctg cactgatatt
    11701 gataatcgaa ataccagctg ctaacttttc atctatttct gtatatttcg acgttaccgt
    11761 actgataact ggaatatcaa ctactcgaca aacttctttt actgtctcta aaggcgtagg
    11821 accgttcaaa acgaccccta acgaaccttg cgcttctgcg aacatgctca tgtaggaaga
    11881 acgagcacct tgtgtcaaac caccacctac accggagaaa actggaatgt cagctgcctc
    11941 aataatgctt ttagtaatcg ctggatgagg tgtaaaagga tacacagcaa ttactgcatt
    12001 cgcatttgta ttacgtatga tcgcaatatc cgtagtaaag atgatcgaac ggatcaattt
    12061 tccaaatatc ttgatcccgc ttgcttctaa aatgatttcc ggtactctta cgatatcttt
    12121 tctcaattcc gtcatgattt ctggcgtaaa ttgttctgcc atcagccact tctcctttgc
    12181 tatctccggt catactgatt caattgatac gtctcgatga cctggatgat tttttgcgca
    12241 taaccggggt ctgtcgcata acctgcatct tgcaatgctt gtgcggcttc ctgatagttg
    12301 gtggccgtaa tcactttttc atacagcgca ggattccaat ctactccttg gacgaacagt
    12361 tgtgtatggt catccatgga ctgttcccat gaatcataca ctttgaagtc tccttggata
    12421 gttatccatt cttcgttaac aaattccttt gtttccaggc ttactttctt ctgatcccca
    12481 taagccttga tcccgaaaag attattgtat ttgctagcca aggtactttt tccccaattt
    12541 gattctaata tagcttgtcc taaaatgata ctaggaagca ctccataacc atcttgtagt
    12601 tcttgggcat gcggaacaag ccgttcaata aattgctgat gggattgcgc ttcattttct
    12661 tgctgattat cagtaaaagg agaagacaga ttcttcaagg aaaaaacaaa ggctaagagt
    12721 atcactgcta caccggcaac cactgctggc caacggatat tcttgcgctt ttgctttttc
    12781 cacttttttt tcgccatgat caattcctac ttttcattta attcattcag atattcttct
    12841 agcgtcaatt ggtgtttggt gatatactca gcgttttctt taccgacata acgtaagtgc
    12901 caaggttcat atgtgatctt agttatatct tcgtgtcctt ttggataacg aacgataaat
    12961 ccatactgcg cagcattatc tgcgatccat ttagctccag gctgatctcc gtaacttgca
    13021 tccaatacag tagaaggata actgttgtac cagttttgat cgacgacatc gactgccaga
    13081 cctgtgtggt gttcgctgta tcctggctcc gtaatcgttt ctttagtaat ctttgtcgcc
    13141 tcctcttctg atacaccttg acgactcatc acatcttgga cattttgttc gaaaacttgt
    13201 tgttgggatg atacagaccg gtaagctgag accatcacta atggaaatcc agctgcttca
    13261 gcagccttgg caaatgcttc atattctgga gcaatgcgtt tgtcgatcaa gtatccgtta
    13321 cttaatgatg ccaattgagt agactcatca atttcttttt ctaatttgtg atctggacca
    13381 accaaaacta gcttccaatc gtctgtttta acatctggca attgtttgga cgtacgatct
    13441 tttgtttctt tgtcctttac cttgctgctg gttgtttgac tgttcttttc tttactagca
    13501 gttgttttgg tgcttgttcg ttcgactgct tgattgccgg cttgaacatg cgaatcatca
    13561 tcgctaaaaa taacatagcc cattgctgcg atcataatca caactgctcc aaatcctaaa
    13621 aatttattca cttgaaaaat cctttcgttg ttcttcacgt tccccattcg cctcatgaac
    13681 gtaagtttct tccagatttt cattcaccca atcgagaagt tcgatttttt ctccttcgat
    13741 ctgtttttga acatctgctg gcagacgaaa attcaaaata tcatcatagc cccattcatc
    13801 atattgaata cttactttca agtcaaggta tctaccagcc tgctgattat ttttcaaagg
    13861 gatcagctcg acttttgctg ctttgctgct aagccatttt tgcgcgatgc gtgttttgat
    13921 ttgcttgtac tctgcaacgg caacgtttcg tttttcggga aaaataatcg tttgacgcaa
    13981 aatcttttgc gttagcatcc attcataatc agtgaaaaga ttttttactt tctgcatcgc
    14041 ttcagatggc agatttttct cgccatcttt ccatttttgc cattgttctg gactgatcag
    14101 caagtattca tctaaaaaat ttttttcagt ttcaaactgt tctaataata cactcataat
    14161 aagatcaata tatacttgat gcacgtttat cccctccttt taactattct acttaagttt
    14221 tgatcaaaaa gcacctctta aatcgctgct ttcatttttt ttaatgtttc aagtcctctc
    14281 ctctttattc ttcgtgattt ttgttataat aatagacaga caagaaactc aatccaacta
    14341 tgaaggagcg ttttcagatg aaaaaatatc aatggggaat cgttggtctt ggtaccatcg
    14401 cacatgagtt tgccgaaagc ttcaatcaag agacgagcga actaacagca gttgcttcca
    14461 gaacttcaga aaaagcggaa aacttcgctc accgttacaa tattccaaaa gcttacggaa
    14521 gctatcaaga aatgttagac gatgctgaaa tcgacatcgt gtacattgct gtaccaaata
    14581 gacagcatat cgaccatatc ttggcagcat tggaagcagg caaacacgtt ctatgtgaaa
    14641 aagccatcac gatgaataaa aaagaactgg ctgacgctat gcgcttagca gaagaaaaga
    14701 atttgatctt agcagaagcg atgacaatct tcaacatgcc actttatcag cagttacgct
    14761 caataatgga tacaggaaaa ctgggggctt taaaaatgat ccaagcacct ttcggcagtt
    14821 acaaagaacc agaccctaaa aaccggttct tcaatccaga attagctgga ggagctctgt
    14881 tggatatcgg tacgtatgct gtatctttcg ctcgtttctt tttgagctct caacctgaag
    14941 tagttgcctc tacaatggtt ccttttgaaa caggcgtgga cgaacaatcc gtcactatct
    15001 tgcgtaacaa agaaaatgaa cttgctgctg tcagcttgac ttttcaggca aaaatgccaa
    15061 aagtcggtat cgttgccttt gagaacggtt atgtgacgat tgctgattac ccgcgtgctg
    15121 atcgtgcgga gatcttattt acagatggta caaaggaatt catcgaaagc gggaatacat
    15181 ctgaagcaat gaattacgaa ataaagaata tggtacacat gattgaaggt gacttgccca
    15241 atcgctctct cttcttgaca aaagatgtca tcgaaatctt ggatcaaatg caagttctat
    15301 ggaaaaatat gtaattttaa aaggttgtga aaaagctgtt ctgcatcaag taataagaac
    15361 agccaaacaa aaatagctcc tcctattttt cgtttggctg gacttattac cgcagaggaa
    15421 agcttttgaa caccgtttaa gaaagaggtt gtgagcctac cgttcagctc cgagtcacaa
    15481 ggagcaattt caaaaaacag cttctcatgt ttgttgaata ttgtgacttg tgaccgagga
    15541 gttgacagga gaacaccgtt taaagaagag gttgtgaaat cagctttttg acctgaatat
    15601 tagctgaatt cacaacctct atttttggat tttttgttgg gagaattctg ccttgatttc
    15661 aataaaactt ttaaaagact gcaaaaactg gaacaattct gctcgatcct cgaattcagg
    15721 gcgactagtc ggcagaggca tttgccgata caatacataa acttcttcaa tacgtgttaa
    15781 aatttgtctg ccatcatttg actctgaaaa tgtttctgca gtataaatca ataaaccata
    15841 gatatgtttt ccataataag ctggttgctg gattctctct agattttcca gcatgtcttt
    15901 aatcacattg gcctgtgctc ttctcataga gaagtaagtt tcgtagtata gcggctgtct
    15961 gagccagtag ttttcttgat gttctctggc atcttcttgg ctttcgcgaa tgaatgtcag
    16021 taaatgttcg catttgatct gcacatcttc tttattttct gataaaagaa attctgccat
    16081 ttgtctcaaa atatttcgaa attctttttc gatctgtatt tggttttcct tcagctgctt
    16141 cgtacgatct ggcatgtaaa catttgcaag caaagcaaat actaatccaa tcgccattaa
    16201 tcccgcttca ttgccgatca gctgccaaga catgttttcc tccactaaat aatgagtgac
    16261 caaaaccgaa tttactacta tcccttcggt taatttgaag cgagaggcaa gcgggatgaa
    16321 caggaataaa aaaagactga agccaaaaat agtataccca acagcaaaaa aactgaacag
    16381 tgctgttgcc gtagctaata taaaggctgc gattcgattc atgcccgtac gcaatgtaga
    16441 gcgcttggta tttcctacac ttaataatgc aatgatccct gcagctggcc aatacaacag
    16501 cgataagcta cccgctgtga ccatcgaaaa gaagacactg atcactgttt tgactgttcg
    16561 cattcctata cgcatttact tccccctttt ttattttaat tatacttgaa taatgaaaaa
    16621 ctagctatca gacaaaagtt taggtatgat agtaaaaaga ccaaaaagga gaatctctat
    16681 gcaatttggc cgttttcgac taggtatgcg tacgatcaaa agtgccttag ctgttttttt
    16741 atgtatcttg ttttttcatt tgactcaacg aggaatcccg atgattgctg cattatctgc
    16801 tgttttttct ttgcgtcagg acttgactac aaccgtttct tttggaaagt ctaggatcat
    16861 cggcaataca ttaggtggta cattagctat catttatttt tttgtcaaaa gctattttca
    16921 caatgatttt ttggtagaat tgatcttgct tcctattcta gttattattg tgatcgtcgt
    16981 gtcagatggt attaacaata attccggtat catttccgca atagctacgt tgttgctgat
    17041 tgcgttgagc atccctcaag gcgaatccgc tttgtatgct atccaacgtg tactagatac
    17101 ttttataggg acattcattg caatcggtat caatttcttt cttcgtcctc ctgaaacaga
    17161 aaagaaggaa gaactatcag aagatttggt cgaactccaa aagaaagaac gattgctaag
    17221 aacaaattta caagagatag aagctaagat ccaaaaacaa aagaaaaata gcgaggacta
    17281 attttctttt gcaataaaat aaaaaaatgg agagaagaac tttaaaaagt cttcctccat
    17341 ttttgattga acggtgaagt gtactacttt attcttcctt catcgagttt tgttctgcct
    17401 cttttggcgc ttcatacata tttacttggc tagtagtccc acgtgtcgac aaattctcta
    17461 cgatttcctt caagtcaagt cctgttgtcg cttttagtgt ttcttgagta gaagaaagca
    17521 aatttgtggc ataatttgtc actcggttcg ctccactggt ttctcctgaa ctgcttcctg
    17581 tatcgacaac agagattttt tcgatatttc ctagcggctg tgctgcttcg cgcatcaatt
    17641 gcggtagcat atcgataacc atactcaaga cagctgcttc gccatattcc ttgaaggcat
    17701 tggcgatctt ttgttttgct tcggcttctg cttttccttt tgctagtgct gcttcggctt
    17761 ctgcttgtcc agctaaacgt gtcttattgg cttccgcttc tgccagtgct tccacacgga
    17821 atcgttctgc ttcggcttca gtcacttctt tgactttttg agcttgtgct tcttgttctt
    17881 tagcgtaccg atcggcatct gcttttttct tcacttccga atcatattgc ttttcacgac
    17941 gtgtgatttc tttttcttcc agttcgattt gtttttgacg ttctacgatt ttaatctgca
    18001 tttcctgttc gattacttgt tgttgtgctc gggcactttc taaattgtag gcttggtctg
    18061 cttttgcttt agcaacatct tgttcttgtt tgtaggttgc taatttcagt tctttttctt
    18121 ttaatgattc tgcgatctct gtttgtcgct gtaattcagc tgcttgtgat tctttttctg
    18181 cttgagcttt tttgatacgc gtttctttta gagcttccgc ttctgctata tctgcatctc
    18241 ttttgacttg ggcgattctt ggtttcccta aagagtcaag ataaccgttt ttgtctctga
    18301 cctctttgat agtgaaagaa acgatgatca accccatttt tgcaaggtct acagaagcga
    18361 cttcttgtac gctttgactg aacttatcgc gattttgata gatttcttct acagtcatag
    18421 aaccaagaat cgagcgcaag tgtccttcta atacttctct tgcttcattt tccagttctt
    18481 ctcttgtctt gcctaaaaac tgttctgctg ctgtagcaat ctcttctaca gatgaaccga
    18541 ttttgatgat cgaagtacca tcacacatca ccggtacgcc ttgttccgta tacacttcag
    18601 gggtggagac gtccaattta ctcgataata gactgatccg attcgaacgt tgaaaaactg
    18661 gcaagacgaa tgcgccacct ccacgcacaa ttttgatttt gttattgctt tcatcaatgt
    18721 gcacattttt gcttcctaaa taacttccac tgatgatcaa cgcttcatcc ggttttgctg
    18781 tctggtactt cgctacaaag acgatcagca acatcagtaa aatcaaagct gcgattgcga
    18841 tcgcgataaa aacaactggt atttccattt aacacacgct cctttaagct tttatcatgc
    18901 tttcataagg catgacataa cagattcttt ccttgatctc aatgatcaat actttcgttc
    18961 cccgctcagc tgtctgttct tgggggctgt aaaaacaagc tggacgacta atggaaccgg
    19021 taacactttt caactgaatt tctcccatac ctcgtacagg gatcggtgtg atcacaacag
    19081 ctacttgtcc ttccagagtt ttttcagaag acgacatcgt tgattcggca tgtttcatcg
    19141 gcatcaatat atagaaatta agcaaaaata ccaacactat cgatatgcct ccactagcaa
    19201 taaataaaaa taaagatgat tgatctgtta aacgctcacc tagaaaacca aataagcttg
    19261 tgaaagttaa ccaagggata ataagcatcg gatcaagcgg gccatcaaaa tcgaatacat
    19321 ctccaaaaat aatcaatagg actgccaatc ctgcacaaat cagcaatgta taccaataaa
    19381 tcgtctcaag tgcgattccc cctatcatat acacgcctcc ttccatttct acgtctatca
    19441 taccaatgtt tgaaaattct gtatagcttc ttttaccgac tcagtggtgt tttttatagg
    19501 aaggttgtgt ttatcgttga caatttgtta aatttttgtt ttcatgttaa aattttcaca
    19561 ataaattctt tcgaggtagc ctatgtttcc tttttttaag aaaaaaccat taaagctcca
    19621 gaacacgatc atccttgtcg tttgtttctc cacattcgtt tctttgattg tttctgccat
    19681 attgatcagg aattttattc tttcgatgaa ccgatctagt gtgtcagata aaatctctag
    19741 cattgctaaa atcgttgcca atgataaact catccaaaaa aagctgcaga ctggtgaccc
    19801 gacacatgcg attcaaggaa taacaaatga aatcatgatt gcatcgaatt tagattttat
    19861 cgttgtcatg gaccctgaag gcattcgata ttctcacgca gacccaagtt acatcggtct
    19921 tcctttctca agtcatgaag atgtgaaaaa agtgattgga ggacaagaat ttttctctga
    19981 aaaagaagga atgttagggt tcggcttccg ctatttcgtc cctatttatt atgatcaaga
    20041 gattattggt gcagtttgtg ttggcttaac aaacaacacg attgacaatc aagtcaaaga
    20101 tggacaaaaa atgattgttt tttcattgat tattggactt agcgtaggca tcataggcgc
    20161 cgtttttctt gcccaaaaaa tgaaaaaagt actgcttgga cttgaaccac aagaaatagc
    20221 agaacagtta agtgaaaaaa cgattatcga aaatgaagta gatgaaggaa tcttagccat
    20281 ctcttctgat aaaactatcc tcttgatcaa tacacctgca aaaaacattt tctaccaaac
    20341 agagcagcta ttggatttcg aagtaggaaa aaaatcagtg ataatctata tgacgttttg
    20401 tttaaagaga cgattgagca tcaaacgacc gttcggactc aatccatgta tttaaatggg
    20461 atagaaacta tttctagcgt tacacctctt tttagcaatg accgtttttt aggagcagtc
    20521 gcaactttac gaaatcgatc agaagtaatg aaattaatgg aggagcttgg ggggacgaaa
    20581 caatatataa actccatgag agctcaaacc catgaattca tgaataaact tcatgtcatt
    20641 tctggtttga ttgatttgaa gaagtatgaa gaagtcaccc attttatcca gcagctaaat
    20701 caaaattata aaaaagagat tggccaagtc tcaagtctga tcaaagtacc tgcaattgcc
    20761 ggttttttat taggcaaaat aaacgaagcc aaagaacaat ctgttgaaat gctgttagaa
    20821 agcgattctt ttatccctaa aatggagatg gatgactcca tccattcact tttgcaagtg
    20881 ttagggaact tacttgataa tgcaaaagaa gccgtgctac aaaaacatga aagaaacgga
    20941 caggtccgga ttcatttaaa ctatgacaca gaagctgaag tattcatcgt gaccgtctgc
    21001 gatgacggtg tcgggatatc agaagaaatc tcagaaaaaa tatttacgct aggtttttcg
    21061 acgaagggaa tcaatcgtgg gtttggattg aatctcatcc attcaatcgt caaacgccac
    21121 ggtggatttt tagaattttc caaacaaaca aatggcggta caatcgtcta tctagaatta
    21181 ccaaaaaagg aagatcaaaa tgatgacaaa tgtcctgata atcgaagatg atccaatggt
    21241 tgcagaattg aatcgtactt ttgtggaaag catccctaat atgaacgtaa tcgaaagttt
    21301 cagaaatgta gaagaaggaa aaaaatattt cagacaaaaa aacgcagatc tgctgctgct
    21361 tgatgtatac atgcctggcg aaagtggtat ccatttttta tcttggctta gacagcaagg
    21421 aaatgatatc ccagtgattt tgatcactgc tgcagatgat atcgaaacca taaagaatgt
    21481 gatccgctac ggtgccttag attatttgat caaaccgttc actttgcaac gttttcagct
    21541 ggcgatcgaa aagtatcttg ctttctctca gacaatccaa acgactgaaa aagccgatca
    21601 gaaattgatc gatcaagtat tcaaccacac cactcccatt cacgaaatac catcattcat
    21661 cgatttgcct aaaggtctgt caaaactgac tttaaaaaaa ataagtgcat ttattctaaa
    21721 agaagatact attttttcaa caaaagaact ggctcaaaaa agctcactgt cacgtatatc
    21781 cgtcaagaaa tatttgaatt ttttagtaga acaagatata ttgaacgaaa gaatcaaata
    21841 tttggaaatc ggccggccgc taacctgtta tgaattgaca aagaatgggc ggaaaaagct
    21901 cacagattac tgttgagtca tcgaattatc ccatacaaaa tagtccaagt atacagaagt
    21961 aacaactgtt tacttggact atttttaaac attttattga cgaatatcca tgttttctta
    22021 tcttaattga cgggctttag gaagagatac ctcatttttc gtttttccag tttctattat
    22081 ttcacgaaag ttctcgaaac ttgtcgaaat catatttgat aatgcctcat ctgtatttga
    22141 acctacatgc ggggttacaa gaactctagg gtacatttct actaattgct ggatagcagg
    22201 gtctggaatc gtctcccatg gcttgaagga tcggaaaaaa acctctgttt catttgcaaa
    22261 aacatcggtt gcaaaaccag ctaaatgatt gcttttcaaa gctcttaaaa tagcttcatt
    22321 atcttgaagc tctccacggg ctgtattaat caagatgcta ccttttttca tttgagctaa
    22381 aaatccttca ttgatcattt gatcgttttc acctgggata tgaggtacat gaatactgac
    22441 aatatcgctt ttggctaata actcttcaag cgagtggaac gtcaatactt ttttagcagc
    22501 ttctgtctga tgaatatcgt agccgatcac tttggctcct agtcccttaa acaaaacagc
    22561 ttccgtcaac cctatcctgc ctgttcctat gatccctact gtgcaatttc tgatttcacg
    22621 actaaacatt tgctcatcca ctcgaaaatc tttgaaagat gttcggatcg tcgtatacgc
    22681 agtatttctc aaaagcatca tggcaaaagt caaagataac tctgctatcg catttggcga
    22741 atacgatggt acacgggcta ctgtcatacc aaaatcagct gccgcttgta gatcgatatg
    22801 attgactccc acagtccgtg taaaaacata acgaatccca tattcctgca tttttgcaag
    22861 attggtctca tctgctatac aatttcccct caacaataca gcatcatggt cctttgctga
    22921 cacgatattg tcatgtgtca acagttcttc ataaagactg agatcataat ggtaaatatt
    22981 caatttttca aaaaaatcta tctcgttcgg acgaacaccg taacatgcga ttctcatact
    23041 ttcttcctcc atttcttatg aaaataaacc tatgccatga atcatttgta atacaacgat
    23101 ccatacaggt gtcagtacca cagcaaaaat agttgacagt aaggatgcat tggaggctaa
    23161 aagtgcctcc ctatcaaaac tgatagcata ggctgctgcc actgttgcag ctggtgtagc
    23221 catcatgata acgattgttg ccagtgcttc aaaattgact ggtagaatat tcgttgccgt
    23281 caaaactgct agaatcacga tattcaatgc aggtacaaat accactttga ctagactgta
    23341 ataccatgaa gttttttcag atgcagcttt tttgaagctt acttcaccta atgttgcgcc
    23401 gattgctagc cacgccaatg gtgaacttaa accagctaaa taatccatcg gctgaaatag
    23461 ccacggtgca gtctggtcaa ttcgcaaaaa cgctacttgg cttatctctc cttctgcacc
    23521 tgtaactgac acttgtggca gatacccttg gaacagccaa atgaaaaggc cggcaaaagt
    23581 agcgataatg attggattga cgaacatcgc tttgatattt ttagcttcca tcttcaagcc
    23641 actcattttt atgtatccat aagaatacaa aaagattcgg tagccaatat tgaaaattga
    23701 ggaatacatc acgccgacag gaccataaat cgcactgacg ataggcgttc cgaaaaaggt
    23761 cgtagaacca aagatcgtca ggacacgcaa cgttgtttgc ttatcccctt tatagcttaa
    23821 gaaaaaaggt tttgaaacta gaataagaat aatataaata actaatcccc aaatcaagac
    23881 attgattcct tgcatcagta attctgtatt gatatcttgc atgaaagctc gaaatgccag
    23941 tgctggcaaa gccactgtta agacgacttt cgatagaatt tttccaacag attcagaaaa
    24001 aatccccttt tttctacaaa caaaccctaa taatataata aaaactgttg aagtgattgc
    24061 actgacaatc gacatatctg tcaaagtcga tcgaaaaatg tcaaataagt tcatctttct
    24121 cctcctaatt atgttcattt attcacaata aaaaaagctt ttattttgct aatacgtatt
    24181 aacattttta attttatctt tacattgtta cttattgaac aataggaaat taaagaaact
    24241 tttgaaactt atgtaaagtg atttttacta gaacacaaaa aaaaccttaa aaccaaactt
    24301 aagaagttta gttttaaggt taacttaaat tatattatgc gtggaaacgt tccacgccat
    24361 ctagataagt tgcttccaat gtcatatcag ggttcaatac gataaagtct gcatcgcgtc
    24421 cttgagcgat ttttccacat gtatcatcga ttttacagct gattgctgga atcagactag
    24481 ccatcatgac tgcttgttct ggtgttgcta atccccagtc tacaacattt ttaattgctt
    24541 ctttcaattt caatatactt ccagctaagt tgccagattc tagacgtgct gttccttctt
    24601 ttactactac tggaaattcg cctaaattgt aatttccatc aggcatccca ccggccatca
    24661 tacagtctgt aattaatgcg acatgatcat gtcctgcttt ttccattagg attcccgcag
    24721 catttggatg tacatgatgg ccatcacaaa tcaattcaga aaagacatgt tctaaagata
    24781 ataacgcacc caccatacct ggttcgcgat gattcaatcc acgcatacca ttgaaagcat
    24841 ggacaaatac acttgcgcct gcttcaacag ctttttgcgc ttcttctagt gtagcattac
    24901 tgtgtcctag agaaacaact actccttctt cagtcacttg tttgacaaac tcttctacac
    24961 cttgacgttc cggtgctaaa gcaatttttt tgattaaacc acctgaagct tcttgccatt
    25021 catggaaagt atccaaatca ggatccccga aataacttgg gttttgtgcc cctttgtgtt
    25081 cttctgtgaa gaaaggacct tcaaaataga ttcctttgat tttagctcct ggtgcttcct
    25141 gatacatttt gccgattgtc tcagctacat cttttaaacg ttctttgctt gacgtcaatg
    25201 ttgttggtaa aaatgaagtc actccacaag ataacagacc ttcagacatc actttgatgc
    25261 cttctgcatc attatccatt acatcatgat tcatatagcc gtgaatatgt gtatctacca
    25321 aaccaggagc aatccatttg ccgctttggt caatgatgtt gacttctccc tcaggtagtt
    25381 ctttggtgta ttcaccaaaa agtccatcgg taatttcaag atagcctgca tgtttgacac
    25441 ctgacgctaa aaagaatttg tcagcataga taaaagttct cattctattc tctccctttg
    25501 tttttctacc tctaaagtac tcttatttag agaagaagtc aagaatggtc tacaccattt
    25561 acaaattttt tgaaatttgt ttcacagctt gtgctaagcc ttcatagaat gctgctcttt
    25621 ctggttcttc tttacgcttg tatattaagc ttttaagcac aaataaatca ttgaggtaat
    25681 agtagctatt cttctcttga gcccaataga ttccttgatt cacctgttct aacgcttcgt
    25741 cgatttcatt ttgatggaat aagaaatttc cataattata aaaaatttgc gaaaattcac
    25801 tttttgatcg ttcatcagct gattgatgca gcagttggat gcttcgactc aaatagtagc
    25861 gtgcttcagc cgtttttcct gccacaccat atatttttcc gatacaacta atcagttgta
    25921 tttctgtgtc agaaacatgg attctttctt tttggctaga gtagcttaat gcttctttca
    25981 aataataaag ggactgagag atattttgac gcaacgtgaa ttcgcaaaca ccgagataat
    26041 aataatagcg ttgaaagtca gttgcttgat acagattttc agaaatatca gaagatttta
    26101 gtacttgttc taacttccga taatcacgtt tttggaaaca actatccaac agttcaaata
    26161 atttatcgtt tgtccgagta gtttttcgag agagattcat cacatgatca atcgttaccc
    26221 ctagccgttg acaaatctgc atcatcacca atacattcgg cagttcctca ttattttcga
    26281 tccgacttaa tacactttgt gaacaaatat catgcgaaag cattttttgc gtcatttttt
    26341 gttcttttcg aatctcttta atgatcgttc caaaggactc gattaacgtt tccataagta
    26401 tcctccccaa aatatgcgta ttcgcattct attcgtattt tactttagga agtcgcaaac
    26461 ttcaagaaaa gacttgcaac taaataatat actttagtta tttaagaaaa ctccactaga
    26521 aagagagcct cagtgtgttt gtctgtggcc ctcctactga aaaatttggt ttttaaaatc
    26581 atttttggcg ataggttgct aggaattctc tcattttctt agctgtttgt ttcaaatcat
    26641 ccattttagg aagataaacg attcgaaaat gatctggctg gttccaatta aatccaccac
    26701 catgtacaag tagaatatga tgttcatgta aaaagtccaa aacgaatttt tcatcatcca
    26761 aaatattgaa tcgctttgta tcaatttttg ggaatatata aaaagcagct tttggtttta
    26821 cggctgaaag accaggaata tcattgattg cattatagat atattctctt tgttcataga
    26881 tccttccgcc tggcaggagc agctcatcga cactttgata accgcctagc gcggtttgga
    26941 taatttgctg agacaataca ttggaacaca aacgcatgga ggacaacata ttcagccctt
    27001 ctatatagcc tttgacgtgt gacttatctc cacttaggac catccagcct actcgaaatc
    27061 ctgctacacg atgagatttg gatagaccgt tcagcgtaac aacgaacaaa tcaggcgcta
    27121 atgtagcaat cggaatatgt tctagaccat ccatcactaa gcgatcatag atttcgtcgg
    27181 aataaataat caggttattt tgtcgagcta cttcaacgat ttcttctaat aattcttttg
    27241 gataaagtgc cccagtagga ttattaggat taatgataac aatagctttt gtccgactcg
    27301 ttatttttga tttgatatca tcaatatcag gataccattc tgcctcttca tcgcaaatat
    27361 aatgaacagg tgtgcctcca gctaatgcaa tagaagccgt ccacaacgga tagtccggca
    27421 ttggaaccag tacttcatct ccattgtcca gcaacccctg catacacatc gtgatcaatt
    27481 cgctcactcc gttgccggtg tagatatcat tgatcgtcac attcggaaaa tgtttcagtt
    27541 ggcaatattg ctcaattgct tttctagcag aaaagatccc cttggaatct gagtagcctt
    27601 ctgagtcacg ggcattcatg atcaaatcac gaatgacttc gtttggtgct tcaaatccaa
    27661 aaggtgcggg atttcccgtg ttcaatttta aaatactaat gccctcttct tgcatccgct
    27721 ccgcttcttc tagaacggga ccgcgaacat cgtaactgac accttctaat ttgttggatt
    27781 tttcaaattc tctcatcact tagccaatcc ctcatttcaa aaagttagaa ctttccaaat
    27841 tttttgttat ttactatttc aatatcatac tccatcctag aaaattttgc cacttattta
    27901 ggaaaaacgt aagtattaaa aaaacacaaa caagcgttga gaacttaatt gtcatgatag
    27961 tcattccata gattcgctgc ccaatccatt gcaattccac aaatgattcc tagcataaaa
    28021 ccaagcaaaa aacgtccgaa tgcaagtcca atcaatacac ctaagatcat tcccagtcct
    28081 gaaaattctc cgtcatattt ctccatctta ataagtcctc ccatttttga gcttgacaac
    28141 tatgtaaaac ctcgctatca ttagtgtgtt attattacgc agatgtgttg gaattggcag
    28201 acaagcatga ttgagggtca tgtgagcgtt agctcgtgtg ggttcgagtc ccatcatctg
    28261 cactacaact cgtgaagtta attttgcttc acgagttttt ttgtttgaac gtttctttat
    28321 gccattatat acatatcact gtatccttta caaaaagaac gtacaatcat gtaaacagcc
    28381 tgaggtgagt atatgaaggt cggaaacaag ctactcgtag aaacttgcaa gtgttaaaac
    28441 aattaatcga aaaacgagta aaatccctca cctaatacta aagcgaggga ttttttgtat
    28501 attatctgtt tatcaccgtt attataaagg tacaaatacc aagcacggtt aatccaaatt
    28561 gcataaattc atttttttgt acactcatgc ttttcattag ctctttaact gccattagta
    28621 ctaagatggc gataacaaca taaagtatat agtacgtccg cattccggaa gcccatactg
    28681 tgggagaaaa tcccataatt aaccttgaac aaaatcccat tatcatcaaa ataactaaaa
    28741 aaatagaagt tttcttattg tcaaaactta aataaattcc aacaattaaa catagtaata
    28801 atgccaagac tactaaagta gctatccaag tacctggata aaaaagtgac agtttcgttc
    28861 ctaactctgt aaagagatta ttcaggttac tactactcca aatcattgcc cttttatttc
    28921 cattgacgta agtaaagctt tgacccattg tattaccaaa ataaataatc aggtttaaga
    28981 aaaacggtaa tgcagtcaat atacgcacat aatagttttg acatttccta tatgttagaa
    29041 ggaaaattaa gaaaaatagc aataaaaata agatattcat gtctaaaaaa aaggctctat
    29101 actttttgat gttggtgaac ttcaccaaca tcttttttgt a
//
LOCUS       DAZUKC010000032        28045 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000032 DAZUKC010000000
VERSION     DAZUKC010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.033"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            25..696
                     /locus_tag="ACG80T_001331"
     CDS             25..696
                     /locus_tag="ACG80T_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS4840991.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            755..1195
                     /locus_tag="ACG80T_001332"
     CDS             755..1195
                     /locus_tag="ACG80T_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4840992.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1207..1875
                     /locus_tag="ACG80T_001333"
     CDS             1207..1875
                     /locus_tag="ACG80T_001333"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFS4840993.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1939..2562
                     /locus_tag="ACG80T_001334"
     CDS             1939..2562
                     /locus_tag="ACG80T_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFS4840994.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2618..3004
                     /locus_tag="ACG80T_001335"
     CDS             2618..3004
                     /locus_tag="ACG80T_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFS4840995.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3217..4608
                     /locus_tag="ACG80T_001336"
     CDS             3217..4608
                     /locus_tag="ACG80T_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFS4840996.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            5219..5587
                     /locus_tag="ACG80T_001337"
     CDS             5219..5587
                     /locus_tag="ACG80T_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFS4840997.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5767..6708
                     /locus_tag="ACG80T_001338"
     CDS             5767..6708
                     /locus_tag="ACG80T_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFS4840998.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6777..7508
                     /locus_tag="ACG80T_001339"
     CDS             6777..7508
                     /locus_tag="ACG80T_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS4840999.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7652..9832
                     /locus_tag="ACG80T_001340"
     CDS             7652..9832
                     /locus_tag="ACG80T_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFS4841000.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9852..10334
                     /locus_tag="ACG80T_001341"
     CDS             9852..10334
                     /locus_tag="ACG80T_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFS4841001.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            10350..11111
                     /locus_tag="ACG80T_001342"
     CDS             10350..11111
                     /locus_tag="ACG80T_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFS4841002.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            11242..12576
                     /gene="murC"
                     /locus_tag="ACG80T_001343"
     CDS             11242..12576
                     /gene="murC"
                     /locus_tag="ACG80T_001343"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFS4841003.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12883..13368
                     /locus_tag="ACG80T_001344"
     CDS             12883..13368
                     /locus_tag="ACG80T_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFS4841004.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            13361..14044
                     /locus_tag="ACG80T_001345"
     CDS             13361..14044
                     /locus_tag="ACG80T_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFS4841005.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            14170..16815
                     /gene="polA"
                     /locus_tag="ACG80T_001346"
     CDS             14170..16815
                     /gene="polA"
                     /locus_tag="ACG80T_001346"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFS4841006.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16860..17696
                     /gene="mutM"
                     /locus_tag="ACG80T_001347"
     CDS             16860..17696
                     /gene="mutM"
                     /locus_tag="ACG80T_001347"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFS4841007.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17660..18289
                     /gene="coaE"
                     /locus_tag="ACG80T_001348"
     CDS             17660..18289
                     /gene="coaE"
                     /locus_tag="ACG80T_001348"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFS4841008.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            18341..18673
                     /locus_tag="ACG80T_001349"
     CDS             18341..18673
                     /locus_tag="ACG80T_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFS4841009.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18985..19476
                     /gene="nrdR"
                     /locus_tag="ACG80T_001350"
     CDS             18985..19476
                     /gene="nrdR"
                     /locus_tag="ACG80T_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFS4841010.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19499..20872
                     /locus_tag="ACG80T_001351"
     CDS             19499..20872
                     /locus_tag="ACG80T_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFS4841011.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20872..21798
                     /gene="dnaI"
                     /locus_tag="ACG80T_001352"
     CDS             20872..21798
                     /gene="dnaI"
                     /locus_tag="ACG80T_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFS4841012.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21927..22367
                     /locus_tag="ACG80T_001353"
     CDS             21927..22367
                     /locus_tag="ACG80T_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFS4841013.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            22511..23212
                     /locus_tag="ACG80T_001354"
     CDS             22511..23212
                     /locus_tag="ACG80T_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFS4841014.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            23209..24330
                     /locus_tag="ACG80T_001355"
     CDS             23209..24330
                     /locus_tag="ACG80T_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFS4841015.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            24345..25577
                     /gene="pepT"
                     /locus_tag="ACG80T_001356"
     CDS             24345..25577
                     /gene="pepT"
                     /locus_tag="ACG80T_001356"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFS4841016.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            25709..26617
                     /locus_tag="ACG80T_001357"
     CDS             25709..26617
                     /locus_tag="ACG80T_001357"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS4841017.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            26652..26924
                     /locus_tag="ACG80T_001358"
     CDS             26652..26924
                     /locus_tag="ACG80T_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFS4841018.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            26935..27981
                     /locus_tag="ACG80T_001359"
     CDS             26935..27981
                     /locus_tag="ACG80T_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFS4841019.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         9433 a         4844 c         6111 g         7657 t
ORIGIN      
        1 aatgatgaaa aaagggggag aaaaatgaat tttttagttg ttttaaaaga tactctgatt
       61 gagcgatcta catttctata tgttcaattg caggaaaata aaacattgct gcatttttca
      121 ccagagttcc atctagaggg gctaacatta ggagaaatct atcaagcaga agggctgtcg
      181 gctgttttgc acttttttaa ggtagaattg gaattaccag taaaagatta tatcgagctt
      241 tctcttgaat cttgggatcg agcagtggtt gaactgtttc cagaaggttt aatgatcgat
      301 acaaacgatg ggaagatcca tctgactgca gcagaactgc aaaaacaaat gcgttatcaa
      361 atagatgacg aaaattcttt ttctattttt agacgtcagc aaaagattct aaaaagcttt
      421 ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat
      481 ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt
      541 atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga
      601 gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat
      661 gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt
      721 gtcataataa atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct
      781 aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca
      841 gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc
      901 ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct
      961 atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag
     1021 agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag
     1081 ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg
     1141 acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag
     1201 aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac
     1261 tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc
     1321 ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa
     1381 cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata
     1441 ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat
     1501 aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa
     1561 gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat
     1621 ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa
     1681 gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat
     1741 agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc
     1801 gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac aattctggac
     1861 aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag
     1921 gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat
     1981 acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg
     2041 gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa
     2101 tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg
     2161 gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc
     2221 ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa
     2281 catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa
     2341 cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg
     2401 atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact
     2461 tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat
     2521 ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag
     2581 atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga
     2641 agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt
     2701 gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat
     2761 cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt
     2821 gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga
     2881 agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt
     2941 gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga
     3001 atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta
     3061 ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact
     3121 gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc
     3181 gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta
     3241 gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca
     3301 aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt
     3361 caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt
     3421 gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg
     3481 atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact
     3541 gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca
     3601 atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc
     3661 tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta
     3721 tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt
     3781 ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg
     3841 ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg
     3901 atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt
     3961 ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac
     4021 gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat
     4081 ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca
     4141 agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat
     4201 gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc
     4261 gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt
     4321 ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga
     4381 gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta
     4441 gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta
     4501 aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat
     4561 cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt
     4621 tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa
     4681 atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg tgaataagag
     4741 gttgtgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa tagttcctca
     4801 tattttacgt ttggttgagc ttattaccgc agatgcaagc ttttgaacac cgtttaagaa
     4861 agaggtcgtg agcctgccgt ttcactctga gtcacaaaga gcaatttcaa aaaatagctt
     4921 ctcatgtttg ttgaatattg tgtcttgtga ccgaggagtt gtcaggagaa caccgtttac
     4981 cagaggttgt gacttgtttt tagtcacgac ctcttttttt atttattcca tataaataca
     5041 cattatttat tccgaaaagt gaaagcgcaa ataacaaaaa caaccaactt atttattatt
     5101 aaagaaaagc gtatttaagt aggacgattc tgttgagagc tttcgtagat tttcccattt
     5161 tcctatggta aactgacaaa gttgagaatg ttagaacaga tcgtttagga ggaaacagat
     5221 gtttacgaat gaaataaaaa tcatgcttta tgtggacgat gtagaaaaaa atgcaacgtt
     5281 ttggagagca gttggctttg cggaaaaaga tcgtcaagaa atggatggga cgcttataat
     5341 tgaagtagga gttgaggaca gtcaaacctc atttctgtta tatgatcgag catttgttga
     5401 gtcccattca cctggcacag ctactagtgc cccatctttg atgttttaca gtaaagatat
     5461 ctttggacta tataaaaaaa tgcagacgca atcagtcaca ttaggtgata tggtacagct
     5521 tggagaagaa tatgtcttta actttgctga tcccgatggc aactactttg cggttacagg
     5581 taaataatag aaataaagac tttgtttttc tgtgcgaaac aggaaagcga agtcttttta
     5641 attcaaataa taatattatg taaacaaaaa actttccatg cgtaattata cacaagcaaa
     5701 taaatcgtca aatgcgaacg ccttctttat actgtttagg aacgaaaaga aggaaggaag
     5761 aaatacatgg attatcaagt actgctttat tacaattata caaaaatcga agaaccagag
     5821 gaatttgcca aaaatcattt agccttttgc aaatctttgg atcttaaagg tcgtattcta
     5881 gtagctgaag aaggaataaa tggtactgtg tctggaaccg ttgaaaacac gactgcctat
     5941 atgaaagcaa tgaaagctga tcctcgattt gctgatacga tgttcaagat cgatccaagc
     6001 gaaggacatg cctttaagaa gatgtttgta agataccgtc cagagcttgt aagtttaaag
     6061 ctagaagagg acattgatcc aaaccaagtg acaggtgcct atctttcacc gcaagaattt
     6121 aaagaagcta ttttagatga acagacagtg gtgattgatg cacggaatga ttatgaatat
     6181 gacttgggac attttcgagg ggctgtacgc ccagatatac gcagttttcg agagcttcca
     6241 gactggatcc gcgagcacaa agaagaattt atggaaaaac gggtagtgac ctactgtact
     6301 ggtggcattc gttgcgagaa gttttctggt tggcttgtcc gtgaaggatt caaagatgtc
     6361 ggacagctgc atggcggtat tgctacttat ggtaaggatc ctgaagtcca aggagaatta
     6421 tgggacggca agatgtatgt gtttgatgaa cggatcgctg tcgatatcaa ccaagtagat
     6481 aaacaaatcg tcggtcgcga ctggtttgac ggaacaccat gtgaacgata tatcaattgt
     6541 agcaatcctg agtgcaatcg tcagattctt gcttccattg aaaatgaaga aaaacattta
     6601 ggagcatgta gcatagagtg tgcgaaacat ccgcgcaatc gctatgttca agaccatgga
     6661 atctctccag aagaagcaga actgcggttt acagctttaa ttaaatagaa aacaatagtt
     6721 ttgattgtcg accatttcca atatgcgcta tactagcgtt gaggatggtg atccacatga
     6781 aatcattgat ttatgctatt ggggatgtcc atggcaaaca tgagcttttt gaaaaactta
     6841 ttcactatta tgatccaaag atccatcagc ttgttttatt aggggatctc aatgatcgag
     6901 gtcctcgtac aaaagaatgt tttttattag gaaagcaatt agtcgaagaa acaggagcca
     6961 tttacctccg gggaaaccat gaagaatatt ttttgcaatt tctgcaatca ccggaggact
     7021 ggtatgcgcc ttatatgaga aatggtggaa aagaaacgtt tgaaagcctg attcacccag
     7081 gggcatcgga ggaatactcc ccaactgaaa tctcgatgat gatccaatcg agatattcag
     7141 aactgattaa atttttagtc cagcgcccct tgtattatga atgggaaaat tatctttttg
     7201 tccatgccgg tgtggatttg acaaagaaag actggcgtaa gacaagtcta agagacttta
     7261 tttggataag ggaaccattt catgaaggga aaaacaatac aggtaagaca attgtatttg
     7321 gacatacgat tacaccaatg ctccatggag atatgcagac aacagacctc tggattcagg
     7381 atcataaaat cggaatggac ggtggggctg tattcggagg gtcattgcat ggggttgttt
     7441 ttgacaagga aggtattctt caagatattg aatatcagaa caacgaagga ccttggcatg
     7501 gaatctgact aatggaaaga atgaaggagc tgtgactttg ttgcggctct ttttattttt
     7561 atagagaata ttgtttcttt cagtagttta acccaggata aaaagtatga tagaataaag
     7621 aagcaatttt tagcagaaaa gaggcatttt aatgacggaa aatttgaacc agacgatcat
     7681 tcagcttgta caaaaagaac tttcagacta tcgtcctaag cagttgacta ctgtacttaa
     7741 tttgttaaat gaaggaaaca ctgttccttt tatcgcgcgt taccgaaaag aaatgactgg
     7801 aagtcttgat gaagtccaga ttcgtgaaat cgaagagcgg tatgcctatc ttgaaaactt
     7861 agaaaaacgt aaaactgaag tgattcggct aatcgatgaa caagggaaac tgactcccga
     7921 gttagaagca gaaatcaccc aagccgtgaa aatgcaacaa gtggaagatt tgtatcgtcc
     7981 atacaaacaa aaacgacgga caaaagcaac gattgctaaa gaaaaaggac ttgaaccact
     8041 cgcgaagtgg ctgatgcagc ttacagatgg agaggtgcaa tctgaagcag aaaaatatat
     8101 tgacaaagaa aaagaagtct cttctgccga agaagcgttg cacggtgctc atgagattat
     8161 tgctgaacaa gtcagcgata atgcaaaatt tcggacttgg attcggtctt atacgtataa
     8221 caaaggtatg tatgtcagca atgtcaagga tgagcaagca gatgaaaaag gcgtatatga
     8281 aatgtattat gactttgcag aacccgtcca taaaatggtt tctcatcgaa tattagctac
     8341 taatcgtgga gaaaaagaag atattctgaa agtattcttt caagtagatg aagcagcaat
     8401 tttagcttac ttggatcgcc aaatagtcaa aaatcctgcc tctccttctt cttcattcgt
     8461 cagagaagcc tatcaggata gttataaacg ctttattcag ccagcaatcg agcgagaact
     8521 gcgcaatgaa ttgactgaaa aggcagatga gcaagcaatc gctattttcg gcgaaaacct
     8581 ccgtaatctt ttgttgcaac cgccactaaa aggtaaagtt gtcctgggat ttgatccggc
     8641 ttatcgtact ggatgtaaac tagcagtcgt agatgcgaca gggaaagttt tagcaatcga
     8701 agttatttat cctcataaac ctgcagcgca agcaaaaaga gaagctgctg gaccagcttt
     8761 tatccaatta attaataaat atcaagtaga tatggtagct atcggaaatg gtacagccag
     8821 tcgggaatct gaactttttg tggctgagca gttgaaatcc gccgaccata aaacctatta
     8881 cgctatcgtc aacgaagcag gcgcttctgt ttactccgct agcgaaattg cacgaaaaga
     8941 atttccgcat ctacaagttg aagaaagaag tgctgtttct attgcccgtc gcctgcaaga
     9001 tcctttagct gaattagtca aaatcgatcc taaagctgtc ggagtaggcc agtaccaaca
     9061 tgacgtttct caaaaacgtt tggctgaaca attggatttt gttgtggaaa cagcggttaa
     9121 ccaagtaggt gtggatgtga atacagcaag cccgcagctc ttgcagcata tatcaggatt
     9181 gaataaaaca actgcccaaa acattgtcat ttatcgtgaa gaaaacggag aattcaccgc
     9241 acgaactcaa ttgaaaaaag tgccacgctt aggaccaaaa gcatatgaac aagcaatcgg
     9301 attcttgcga gtccctggtg ggaaaaacat tttggacaac acaggtatcc atcccgaaag
     9361 ttattctatt gcaaaagaaa tattaacttc agtccaactt tcagaaaaag agcttggtac
     9421 agaagaagca gtagaaaaat tgacacgatt atccgtagag aaactggctg aaagcctatc
     9481 agtcggagaa gaaacgttag ccgatatttt agctgggctg acacaacctg gacgagatat
     9541 gcgtgatgag atgccagcgc ccttattacg tacagatgta ttgagtatgg aagacttgaa
     9601 accagggatg gaattgactg gaacggtccg taatgtcatt gacttcggcg catttgttga
     9661 tatcggtgtg aagcaagacg gacttgtcca tatatcgaaa ctcagcaaaa aattcgtcaa
     9721 gcatcctaca gatgtcgtgt cagtaggaga tatcgtaaca gtttggatag aacaagtcga
     9781 tacgaaaaaa ggcagaatca gtttgacgat gctttcccct tatgaagagt aacaatgagg
     9841 agtaacaaaa attgacacaa gaagaactgc agcagttagt agaagagata tcattaaaaa
     9901 gcttccagct tcctttcaag caccaagcaa tgttcaatcc acgcttacgt acaactggcg
     9961 ggcgctatca tttaaacgat catcatttag attttaatgc gaagctattt gaagagtcag
    10021 acgaagagac gattgcagga atcatcaaac atgaactttg ccattatcat cttcatctag
    10081 ctggaagagg gtatcagcat aaagatatgg atttcaagta tcttttgaaa aaaacaggag
    10141 ggttgcgcta tgcaccagcc ttgacgaaga aacagataaa gatggagtat tatcgatgcc
    10201 aagattgttc tgccacgatc tgccgcaaga gaagaatcga tgtacgcaaa tatcgttgtg
    10261 gaagatgtca tggagaaatc aaatggattg agacaaaaag agaagacttt aatgaagcac
    10321 aaaaaatcag atgaaaggat agaaggcctt tggaaaaaag tgagcagagg aatctaaaag
    10381 actggaatca gctattaaat tttttagccg cacctgaaga gggaaatttt gaggaactga
    10441 cggcacctac acttatttta gctggaaact gtcttcctat cttagctgat aaagcagcac
    10501 aaatgtattt aaatggccaa gtagatcaat tgtttttggt cggaggggtg gggcatgcga
    10561 ctagaatcct ttatgagaat tttgaaaagc aaggatttca ttttgaagat ggcatgagtg
    10621 aaagtgaaat ctgtcgtcag tatctaaaag aagtctatga tttgcccgat aaggcttttc
    10681 tgatagaatc gaagtctaca aattctggag agaacgccac tttttcactg gagattcttc
    10741 attccttagg agctgtacca gaaaaaatct tattgatgaa tgatccgaca ctgcaacgca
    10801 ggactcgagc aacttttgag aaagtatggc aaaatgaaca aactattttc gcgaatgctg
    10861 tgccatttgt gccggaaatc cttcactttt ctgaagagat catctttaca gcaaaagaac
    10921 tgaatcatca atggcctaag gaatatttcc atgcgctagt attaggagaa atggaacgtc
    10981 tccatgacga tgagaacggc tatggtccga aaggaaaaga ttttattcct cacattgata
    11041 tatcagagga agtttggtct tcttacacca gaattaaaga gtcaataaaa acggattttt
    11101 ctcgtacttg accattttag caagcgaatc ctcctttcca aataaatttg gttgaaattt
    11161 tcaatcgttt ataagataat gaggagcgtg ttgaatgtaa tagctaaaag aatggacgct
    11221 agataagaga agaggattca aatggaaaat caaaataaat tgtatcattt tgttggtatc
    11281 aaaggttcag gaatgagctc cttggctctt gttttacatg aacaaggtct aaatgtccaa
    11341 ggatcagaca ttgaaaaata tttctttaca caaagagatt tagaaaaagc aaatatcacg
    11401 attctgccat ttaacgcaga taatgtaaaa ccagggatga cgatcattgc aggaaatgca
    11461 tttcctgatt cacatgaaga aatccagcga gcaaaagaat taggtttgga agtgattcgc
    11521 tatcacgact ttattggtca ttttatccaa aattatacga gtatcgctgt aacaggctct
    11581 cacggaaaaa caagtacgac tggactgctc tctcatgtat tgagtggtgt ccgtccaaca
    11641 agttatctaa ttggagatgg aacaggacat ggtgatccgc aagcggaatt cttttcattt
    11701 gaggcctgtg aatatcgccg tcatttcctt gcctattcac cagactatgc aatcatgacc
    11761 aatatcgatt ttgatcatcc ggattattat acaagtatcg acgatgtatt tacagctttc
    11821 caaacaatgg ctggacaagt gaaaaaagct atttttgcgt atggtgacga tgcttattta
    11881 cgcaaattaa aagcaaatgt gccgatttat tattatggtg tcacagaaaa cgatgatatc
    11941 caagcacgaa acatcgaacg cacaacttct ggttctgctt ttgatgtcta tcatggggat
    12001 gaatttgttg ggcacttcac tgtaccagca ttcggtaaac acaatatctt aaatgcttta
    12061 ggtgttattg cagtagctta tttcgaaaaa ctggatctga aagaagttgc agaagaaatg
    12121 ctgactttcc caggagtaaa acgtcgattc agtgaaaaga tcgtagcaga catgaccgta
    12181 gtcgatgatt atgcgcatca ccctgcagag atcaaagcaa cgattgatgg cgcacgccaa
    12241 aaatatccag ataaagaaat aattgcagtt ttccaacccc atacgtttac gcgtacgatt
    12301 gcattgatgg atgaatttgc agaagcactt gatttagctg ataaagttta cttatgcgat
    12361 atttttggtt cggctcggga agaacaagga aacgttaaga ttgaagacct tggagcgaaa
    12421 atcaaaaaag gtggagaagt catcaaagaa aataatgtat ctccattgtt agattatcat
    12481 gatgcagtag taatcttcat gggtgctgga gatgttcaaa aatttgaaca agcgtatgaa
    12541 aaacttttaa gcagcacgac gaaaaatgta ctgtaataca attactgcag tgtccttata
    12601 aaaatagaaa gagggggagt ggcaaaaatc ttgccaagcc cccttttttg caacttatga
    12661 taaacggtgt tcaaaagctt gcatctgcgg taataagccc aatcaaatca aaaacatgag
    12721 aagctgtttt ttcttggctg ttcttattat ttgatgcagg acagcttttt cacgacctct
    12781 gtaaactata ggaatctgtt aaatcaaggg agcagaactt gccttttacg ctacttttgg
    12841 tacaataatg tatgattaaa aataaactgg agggtttcta tcttgaacat aggtaagccg
    12901 agaaaactag gaaaaacaat aaatgaaatc gaggaaggcg actcgttatc cttaacagaa
    12961 tctattgaag ataaccagct tttgctgtat ttgggattga cgaatgatgc aaatcctttg
    13021 tatatccaac atgactttgc acaaaaaaca gaatatcaac agccaatcgt tccatcagtg
    13081 atgctgatgg ggatcatcac aagtgcaatt tcaaagcatc ttcctggacc aggatcacat
    13141 gttgtcaatt tttctgtcaa ttttattgaa ccggttttcc attatgaaac actgactttt
    13201 cagtttgagg tcataaaagt agataagatg aaggatgtgg tgacgatttc tgtagaagcg
    13261 gagaatacga acgaagatcg tgtgttggat gctgtcgtta tggtccaacc gccacaagtt
    13321 cctgaattgg aaatagcaat cgaaagtgaa ggtgaattga atgaataatc caagtacatc
    13381 aatgtcaggt atcaatcgtt tttatgcaaa agtttatgga tttttaggta tgggacttgc
    13441 ccttagtgca gtcacttctt acttggctct ttgggtattt cctttgcaat tagcaacttt
    13501 tatcaataat tttccactag gctttgtggg tctgtggatc gttgaactga ttttagtaat
    13561 ggttctaggc gtaaaagcac aaaagaatcc gagcttagca atcggtggat ttattgccta
    13621 ctctcttttg aatggcataa cattagcggt tactcttgca tactacgata ttggaacagt
    13681 aacgcaagct ttcttgacag ctactttgat gttcatgggg atgtctgttg tcggagtaac
    13741 aacaaaaaga gatcttagcg gggttggaag agcaggacag gcagctttaa tcggagtgat
    13801 cattgcacta gtcttgaaca tcttcatttt gagaagtagc gtagttgagc tgttcatctc
    13861 cctactgtta gtcgtcatct ttgcagggat tacagcttat gataaccaaa gcatccgtaa
    13921 tatctatgtc cagacaaacg gaacagcaca tagtggaatt gctgtattca tggcattgaa
    13981 gctgtatttg gactttatca acttgttctt ggcgtttctg agaatctttg gacggaacaa
    14041 ctaaaaaaga aaaaaattca tcagttgggg ctataccgaa ttatttggta tggtcccgct
    14101 ttttcttttc gcattccgca ggattctttg ttagaatgaa taagaacttt acagtaagga
    14161 gttaagaaga tgacaaaaaa taaattatta ctcgttgatg ggaacagtgt ggcttttcgc
    14221 gcattctttg cgcttcacaa ttctcttgaa cgtttcaaaa atagaaacgg tcttcataca
    14281 aatgcaatct atgcattcaa tacgatgttt gaaaatgtta tgcaaaaaga gcaaccgact
    14341 catgttctgg ttgcgttcga tgcaggaaaa acgacttttc ggacagaaat gtatgcggag
    14401 tacaaaggtg gaagatccaa aacacctggc gaattcaaag aacaaatgcc atatatacgt
    14461 gatttgttga caggactagg ggtacagtat tacgaattgc ccaattatga agcagatgat
    14521 attatcggta ctttggcaga aaaagtagac aaagatcaat ttgatgtcgt cgtgctttca
    14581 ggtgatcggg atttgactca attagctagt aaagaagtaa aagtggatat cactgtaaaa
    14641 ggtgtcagcg atatcgaaag ctatacacca gaacatgtag cggaaaaata tgatgggttg
    14701 acgccaaaac agattattga catgaaggga ctagccggag atgcctctga caatatcccc
    14761 ggtgtgacta aaatcggtga aaaaacagct atcaagctat tgaaacaata tggctcagtc
    14821 gaaggaattt atgaacatat cgatgaaatg aagcaaagca aaatgaaaga aaatctgata
    14881 aatgacaaag agcaggcttt cttatcgaaa aaattggcga ccatcaatac ttcagcgcct
    14941 gtggatgtct ctatcgactc attgaagtat gaagggaaaa atctcgataa actcgttccg
    15001 ttttataaag aaatggattt caatcagttc ctttctaaat tgaatatcgt agaagaagaa
    15061 gtaaaaatgg atgatatttt gtttgaagtc gttcatgaat tcaaagagga aatgtttact
    15121 acggatatgg cgttgtacgt ggaaatgatg ggcgacaatt atcatacaga ggaaatcgtc
    15181 ggagtagcgt gggggacaga aaagaaaatt tatgtcacga acgatctatc tgtattcgaa
    15241 aaccgagcgt tccattcttg gatcactgat ccgactcggc tgaaaaaagt atatgatgcg
    15301 aaacgaactt atgttgcttt aaaccgctat actggaaaaa cgaaaggaat cgattttgat
    15361 gtcttgctag gtgcatacct tctcgatacg aatgaaaaaa gcacagatat cgaaggaata
    15421 gctgctcatt acggatacaa cgatatccaa tcagatgaag ctgtatacgg caaaggggcg
    15481 aaaaaaggtt taccagaaga agaggaactc ttttttgctc atttagcgcg aaaagtagct
    15541 gcaatcaatg cgttgacacc aaaattgagc caagaactgg cagaaaaaaa ccaagaagat
    15601 cttttccgga aaatggaact gccattgtct atcattttgg cagagatgga aataagcggg
    15661 atcaaagtag atgcgactcg cttacaagaa atgaagggcg agttctctgc tcgcttaaga
    15721 gagatcgaac aaaaaatcta cgaagaagcc ggcgaagaat tcaatttgaa ttcaccaaag
    15781 caattaggag tgatcttatt tgaaaaaatg ggattacccg ttatcaaaaa aacaaaaact
    15841 ggatattcca cagccgtaga tgtactggaa cagcttcgtg aacaagcacc aatcgtagag
    15901 gatatcctca cctatcgtca aattgcaaaa attcaatcta cctatgtcga gggactgctg
    15961 aaagtgatcc aatcagatgg aaaagtgcat acccgttatg tccagacatt gacccaaaca
    16021 ggacgattaa gctctgttga tcctaatttg cagaacatcc cgattcgatt agatgaaggg
    16081 agaaagatac gtcaggcatt tgttcctaga gaaaaagact ggctgattta ttcttctgac
    16141 tattcacaaa tcgagttacg cgtacttgcg catatctcaa atgatgaaca tttgaaagct
    16201 gccttcctag aaggtcaaga tatccatgcc agcacagcga tgcgtgtatt tggaatcgaa
    16261 aaggcagagg atgtcacacc aaatatgcgt aggcaggcaa aagcagtgaa ttttggtatc
    16321 gtgtatggca tctctgatta cggactttca caaaatctag gaatcagccg aaaagcagca
    16381 cagcaataca tcgatacgta ctttgaaaaa tacccaggtg tcaaagaata tatggaaaga
    16441 atcgtacgcg aagcaaaaga ccaaggctac gtcgaaacgc tgtatcaccg ccgtcgatat
    16501 ctgccagaca tcaattcaag aaattacaat atccgttctt ttgcggaacg aacagctatc
    16561 aatacaccaa tccaaggaag tgctgctgat attttgaaaa tcgcgatgat cgaattggat
    16621 aaacgattaa aagaaacagg tcttcaagct acgatgctgt tacaagttca tgatgaactt
    16681 gtcttcgagg taccggaaaa agaacttgaa tcattagata aattggtgaa ggaagttatg
    16741 gaacaagctg tttctcttca tgtaccactg atcactgaca gcagttgggg aaaaacgtgg
    16801 tacgaagcaa aataaacatt gtttaagaca gagcaatata atgaaggtga aaagaaaaaa
    16861 tgccagaatt accagaagta gaaaccgttc gtaaaggatt ggaacggtta gtagtaggaa
    16921 aaacgattca aaaagttcag gtcttgtggc ccaagatcat cgagcaacca gagacaccca
    16981 tctttgaggc ttctcttgtt ggcgaaacca tccaatccat tggacgtaga gggaagtttt
    17041 taatttttca tctcgatcat tgtgaactga tctcacactt gcgaatggaa ggaaaatatc
    17101 aatttacgaa agaaaataca ccaatcgata aacatacgca tgtacttttc ttttttgagg
    17161 atggaagcca acttcgctac aacgatgtac gaaaatttgg tcgtatgaca atcgtcgaaa
    17221 aaggagcaag tgcaacttac cgaggaatca tgaaattggg acctgagcct ttgccagata
    17281 gcttcttatt agcagatttc gcaaatggac tgaaaaagtc ccacaaagca atcaagccat
    17341 tgctgctaga ccaacgtcta gtgactggtc taggtaatat ttatgtagat gaagcattat
    17401 gggaagcgaa gatccatcca gaacaaccgg cgaacacact gcgccccaaa gaggtagaac
    17461 aattacgtct atcgatcatt gatgttttgg atcgtgcaat agaagccgga ggtaccacga
    17521 ttcgcagtta tttgaatgca ttaggagaat caggcggatt tcaagtagca ctgcatgtct
    17581 atcagcaaac tggcaaacca tgtatacgtt gtggaacgcc gatcgtgaaa acaaaagtgg
    17641 cacaaagggg gacgcactat tgtccgaatt gtcaaaggct aaaaatcgca aagtaggatt
    17701 tgttttaggt ttgacgggtg gaatagctac cggtaaaagt acggcagcca aagtctttca
    17761 gtctcatgga tttcctttga tcgatggcga tgtgattgcc agagaaactg ttgagcctgg
    17821 aacgcttgct ctgaggaaaa ttgtatcagt gtttggccaa gaggtattgc agcctgatgg
    17881 gcaactagat cgagggaaac ttgggatgat tgtttttcca tcaaaggaaa aacggcaaaa
    17941 acttgatcag ctattagatc cgtttatccg aaaagcgata aaagatcaaa tcgcctctct
    18001 aagttccaat catcctttag taattgtaga tatcccattg ctctacgaag gtcattatga
    18061 ccactatatg gatgcagtag ctgtcgttta tactacgcca gaaactcagc ttcagcgttt
    18121 gatgaaacgt aatcaactga cgctagaaca agcgcagcag agaattagta gtcaacttcc
    18181 aatcgaagaa aaaaagcagc gtgcggatat tttgttcgac aacaatggaa caaaagaaaa
    18241 acttgtggga caaatcgaaa attggctttt tgctaatcgc tttctttgag tatctgtgtc
    18301 aattttgcta tcctgagtat atagaaagtg agggatagcg atgggatcat taaaaaatca
    18361 aaaggataaa gtcgtaggta acgcaaaaga aaaaatcggc gagatgctca ataaagaaaa
    18421 tctaatcgat gaaggtcgta cgcaatcaga aacagcagaa gaaaaagaaa agcagtacca
    18481 agcaagaatc gatgaaatag aagaccagca ggcagaagaa gaagcgaagc ggcaagattt
    18541 gactcaagaa cgaaaaaatc cgacacctga tcgtagtaaa acaccgctcc cagaaagtga
    18601 ccacattccc gatgagcaaa aaatcgagga acagcgaatg aaacactata caggaatcga
    18661 agagaagtta taaaatagta aaaaatcgag atgtgacttg aagcagatga gaaggtgttc
    18721 atctgcttca agttttttgt gctagatgac taaatcagct tttgtgtaag ttatttctca
    18781 gtaatcaagg tgatcagtat tggaaaattg aaatttagta aggacagggg acacaattac
    18841 gcatgaaagt agaaaaaatt gttcttatat ccgtgttttt tgggtaggac agtcgcgttt
    18901 gtgatttgtt tcgtgttata atacaactag ttgtaaagaa agacacatcc tttatataaa
    18961 atgcgatgca agaggtgaga caaaatgaga tgtcccagat gtcaacataa taattcacga
    19021 gtaatcgaca gccgtcaagc agatgatgga cgtgcgatcc gtcgccgtcg cgaatgtgag
    19081 aattgttcgt tccgattcac tacatttgaa cgaattgaag ctgctccgtt attagtaatc
    19141 aagaaaaacg gggaacgcga agagttcaat cgcgataaaa ttttaagagg actcatccgt
    19201 tcagcagaaa aaagacctgt agctatggaa caaatggaac aaatcgtcaa ccatgtcgaa
    19261 aaccgcgtac gcagtctcgg agaaaacgaa gtatcgacga atctaattgg tgaatatgtg
    19321 atggaagatt tagttgattt agacgaaatc gcttatattc gttttgcaag tgtttatcgc
    19381 cagttcaaag atatgtctgt tttcttaaaa gagttgcaag atatcgtgga taaagcaaaa
    19441 gaaaataata aagagaataa taaagaaaac aattaagaaa caaaaaggag gcatttcctt
    19501 ggaaaatgct tggaaagagc ttcaaccaaa aaatatttac caaggaagaa aagcggctcc
    19561 gctgagagaa gacagcaaga acgccttgct gtatctttat cagccactaa tcggcggaga
    19621 agccttgagt atctatttca ccctgttatc agaaatctct caagaatctg gcaaaggtcc
    19681 tgaagggatg catgcagatc tgcttagtag tctaggctgc ggtatcccgc aattttatga
    19741 tgcaagaaaa aagctagaag gaatcggctt actggatgtt tattttaaag aagatagtga
    19801 tctgggacct cgttttattt atgagctgtt agaaccactc attcctaaag catttttcaa
    19861 agactctgtt ctggcttttc ttcttctgga aaaagttggt gaacgacgat tccagaagct
    19921 tacccaattc tttgaaccta aaaggcatac agcagaggga tatcaaaaaa tcacaaaaaa
    19981 atttttagat gtttatcgct tcaatgaagc agtgtacgct gctaatcaag aaaacatcga
    20041 atcgctgaat aaatcttttg aagaatctga atcagtagca ttacttgaag cagattcgac
    20101 attcgattgg cagtttttga atgattggct ggcaaaaaaa catattgcaa gtcttgatga
    20161 tccaatcatc aaacaagtcg acatgtatca tcagctttac ggtttcgatg aattgacact
    20221 tggcgaactg atcagtcaat cttatgactt tagtgaacaa aaagtatctg aaaaagaact
    20281 gcaaaaagcc attttggcaa tgcagagacg tccggtgaag caaacaaaag aaacagttgc
    20341 ggtttctgaa aacagtaatg aaccctcgtc aaacgaacat ttgacaaacg cagcgcttca
    20401 gctgataaaa gaagcacaga gcattccacc gatgaaatat ctggaagcaa tcaagtcaga
    20461 aaaaggcgga tatgtttcta aacaagaaaa ctggttactg caagatcttg tttctcaatc
    20521 aggtctttcc agtagcgtga tcaatgtttt gctgaattat gtactagtga tcaagaataa
    20581 tgctagctta aatgccagtt atgtaaatac agtggcaaat gaatgggcac aaaagaaaat
    20641 cgccacagca gaagaagcga tccagcacat tcggcaaata agtaatcaag caaaacaatc
    20701 aaaacaaaag aagcaaacaa attatcaaac tggcaagcga aatgttcgac gggaaaagct
    20761 tcctgattgg gtcaaccagc caaaagatga aaaacagatc agcagcgaga aaaaagcaga
    20821 gattgaccgc cgttttaaag aatacctggc aaaaaaggag ggtgatatct agtggaagac
    20881 gtaggcaaag aattgaagag gatcatcagc aaacgcaatt atcaagaaaa gtatgctgaa
    20941 atgatcgctg aagtcttaaa tgatgcggat gttcaagctt ttctaagcgc acaccaagaa
    21001 gaattgacac aagcggatat tgaaaaaagt tacgctaaat tgtatgaatt cgtgcaagaa
    21061 aagcgtaaat atcaaaaaaa cgatccaacg atgatcgctc ccggctatga gccgaagctg
    21121 acgttgaatt ttcattatgt ggatgtcact tatgtaccaa cggaagcatt gttagcacgg
    21181 caaagacaag aagaaatcag aagccgagta aaagcaatgg atatgccaaa agatattcaa
    21241 tcggcttctt ttgaaaactt tgaacgtaca gatggacgag gatatgcgag cttagaagcc
    21301 ctagattttg tagagaaata tactgctgat ccaaaagggt atcataaagg actgtatcta
    21361 gtaggaagtt ttggtattgg aaaaacatat ttattaggag caacagcgag agaattagcg
    21421 attgctggat tcaccacgac gctagtccat tttccgacat ttgccgttga gatgaaacag
    21481 gcaatcggaa aagatcaagt ggcagaaaag ttagatgcag tgaaacgttc acctattttg
    21541 atgatcgatg acattggtgc agatgcgatg agtagttgga tacgagacga agtattcggt
    21601 gttatccttc aatatcggat gcaggaacag ttaccaacct tcttctcatc gaattttaca
    21661 atggatgaat tggaacagca tttgtccgtc actcaacgtg gagatgaaga gccgctaaaa
    21721 gcgaaacgga taatggaacg catacgttat ttgacaaaag aaatcgaaat gactggtcgc
    21781 aaccgacgca atggttgaac agttgacaag tccaactgac atttgaccta gtagtaaaag
    21841 caggtcagcg aatcattata tggacagaaa tgaccggaga caatcaccgc aacaagtaat
    21901 gcagaaactt aaacggagga ttttttatga gggctatttt ttctagtata tctgtagttg
    21961 gtctattttt tcttttagca ggctgcggta gcgcagatag caacacaggt tctgttgatt
    22021 cgattgatgc gggtcaaaca gtaaaaacaa gcagtacgaa aacggagaga caaagcatat
    22081 ttgtcggaac gattgaaagt atcactcaag tagaagcaga agaagaaacg cttcagttga
    22141 aactcgtcga tgtcatggcc aaagaagatc cagcgaatat gggaacgtca ttcagcaatg
    22201 acggagtgat cttaaatgct actgctgaaa aaataagcgt ggacataaat gaattgaaaa
    22261 tcggtgacaa agtccaattt acattagttg agatgccgat catgacgatg tctatcccac
    22321 cgcaagttcc tggagattcg attattgatg tttcattgaa caaataacca ataacatttt
    22381 ttcaaaaata agaaaatgtt gttttataga acgaaaaggt atatttttta aggagatatg
    22441 ggaatttttt tattcctagt ctccttattt tatttggaaa atgctataat tatctagaag
    22501 gagtgaagct atgaaccatt tagatttatc tcagcgctta gccgcagtgg gtgactttgt
    22561 ccctgaatcc gctcgtttag cagacattgg atcagatcac gcttacttac ccgttgcatt
    22621 gatattaaga ggaaaaatcg aatatgcagt tgctggtgaa gtcgttcaag gtccttatga
    22681 atcagcacgt aagcaagtca aaaaagatgg attagaagac cgtatcaccg tacgccttgc
    22741 aaatggtcta gatgctatcc agccacaaga tggtattaca gctgtcacga ttgcgggtat
    22801 gggaggttca ctgatccgag atatcctaga agctggcaag ttgaatcaac gcttaagcgg
    22861 acaagaacgt ttgatccttc agccaaacat cggagagcgt acattacgat cttggctgca
    22921 agaaaatcag tataaaatcc aatcagaaaa aatacttgaa gaaaataaaa aaatatatga
    22981 aatcattgta gccgaaaaag aacaaaaacc agttacttac aatgaaatgg agctgatgtt
    23041 cgggccattt ctgcttgaag agaaaagtga gatcttcaaa tcaaaatgga caagagagct
    23101 tcaacaaaga aaagctatcc gtgaacagct caaacaagca gctaatcaag aggcacgtca
    23161 aaaacaaatc gagcaggaaa tccaaatgat tgaggaggtt ttaaagccat gacaatcagc
    23221 ggacgcactt tcatccaaaa gtttgaaagc tattgtcctc agtggctagc tgaagaagga
    23281 gacccagtcg gactgcatat cggaacattg gataagccaa tccaacgagt aatgatgaca
    23341 ttagacgttc gtccagaagt ggtcgaagaa gcaatcaaga aaaaaatcga tttgctgatt
    23401 gctaaacatc ctccaatctt tcgtcctgta aaacggctgg ttactgatca gccacaagaa
    23461 aaaatgtatg cagatttgtt gaaacatgat attgccgtct atgcagcaca cacgaacatg
    23521 gatattattt gggatggact gaatgactgg ttttgtgaac ttctcggtat tgaagtggag
    23581 agttatttag taaagactca tgagattcat tataaaaaac tggccgttta tgtacctgtg
    23641 gatcatgcac agaaaatgcg tgaagtatta gctgcagctg gtgctggtac acaaggtgat
    23701 tataccggta caagttttac ctcgattgga cacggtcgat ttactccgga agctggtgca
    23761 caaccagcga ttgggaaagt agggaagaca gaacaagttc aagaggcaaa agttgaggtt
    23821 attttgccgg aaactatcga aaaacaagtg attcaagcca tgcggtcagc tcatccttat
    23881 gaggaacctg cttatgatct tttcgctata gatgaaccag tcgaaatgtt tgggttagga
    23941 cgagtaggtg aattgccgca agaaatttct atagaggcat ttgtagaaca agtaaaagaa
    24001 gcgttccaac tagatggact gagaatagtc cagccaaaaa atgcaaaatc ctctgtgaag
    24061 cgcattgcga tttgcggtgg aagcggtgaa aaattttatc cgcaagcaat cgcccaaaga
    24121 gcagatgtct atatcacggg agatatctat tatcatacgg ctcatgatat gcaaagcgct
    24181 gggttgattg caattgatcc cggtcattat attgaatcgt tatgcaagca acgatttatc
    24241 gagaaatttg aatcttggaa acaagaagaa aattgggata ttaacttttt cgtttctgaa
    24301 acaaatacaa acccatttca atttaattaa aaggagagaa tactatgtac gaaaatcttt
    24361 tgccacgttt tttacgatat gtcaaaacag aaacacgctc cgatgcaaca agcaaaacga
    24421 ctccatcgac tcagacacaa gttgcttttg cgcaagaatt gaaaaaagaa ttagaagagt
    24481 taggcttagc tgatgttttc tataatgaag ataacggatt cgttatggga acattaccaa
    24541 gcaatgttga tcatgaagtg cgttccattg gatttatttc ccacatggat acagcagatt
    24601 ttaatgcagt aggagtcaat ccacaaatca ttgaaaacta tgatggcgag tctacgatcg
    24661 tactagatgc ggaagaacgc tatacattga atacaaaaga ttttccaaat ttgaaaaatt
    24721 atgaaggcca cacattgatc acaacagatg gcagtacatt actgggagca gatgacaaat
    24781 cagggattgc ggaaatcatg actgcaatgg agatcttgat caataatcct tctatccctc
    24841 acggtaccat caaagtggct tttggtcctg atgaagaaat cggtgtcgga gcggataaat
    24901 tcgatgttga gcaattcgat gtagattttg cttatacagt cgatggtggt cctttaggtg
    24961 agttgcagta tgaaacgttt agtgctgctc aagcaaatat tacgatccaa gggaaaaatg
    25021 tacatccagg gacggcaaaa gatacaatga tcaatgctct acagttagca atcgattttc
    25081 ataatcagct tccagccgat gaagttccag aaaaaacgga aggatacgaa ggtttcttcc
    25141 atttgatggc attggatggg aatcctgaag aagcttctat gagttatatc attcgcgatc
    25201 atgatcgtga aaaattcgaa gcacgcaaag cacaaatcac acatatccaa gaagagttga
    25261 atcagcgatt tgatcaagaa cgaatcaaag tagatatgta tgatcaatat tacaacatga
    25321 aagaaatcat tgaaaaagat atgagcatcg tggaattagc gaaacaagca atgatcgagc
    25381 ttggtatctc tccagtgatc gaaccagttc gtggaggaac agatggttca aaaatctctt
    25441 acatgggtat tccgacgcca aatctatttg ccggaggaga aaatatgcac ggacgattcg
    25501 aatttgtttc tttacaagta atggaaaaag caacagacgt gattgtaaaa attgcagaat
    25561 tgaacacaaa gagttaaaat cgaaaaagaa cagcttatga caaggcgttc gtcccaatga
    25621 tggaatgtca tctgtctaag gctaaagggc aggacaatgg aattggttgt cccgccctct
    25681 tttttgaaga gtaaatagga ggtaaagaat ggaaaagctg ctagtttttt ataacaaaag
    25741 ttcagggaaa gatgagggtg agaaattagc acgctggttt caagattatg taaaagaaaa
    25801 gcagccagaa ttatatgtag aactagctga aactggccca tcgattgatg atgcggaatt
    25861 agtcaagaaa gcagaagcca tagaagctga tactttagta attataggtg gagacggcac
    25921 gattcatcat atcgtccaag cgttccaaaa aacattatct caataccatg tcggattact
    25981 tccaggtgga actgtcaaca accttgcacg tgttcttgag attcccctca aaaaagaaga
    26041 agctgctgac gtgatcctgg aaggcaatgt acggaaaata gactatggtc aagtcaatga
    26101 tgaagtcatt atctctacac tgacaatcgg aatcttagca gatacagcag tttggatcag
    26161 tcaagaagaa aaacagcgtt acggctcgtg gatctttatc aagaagtttt ttaagttatt
    26221 gctgaagaag aaaaaatatc atttggattt agaaacagac gaggagcatt ggaaaggaaa
    26281 atcccaactg ttgacagtca cgatgtctaa ctccgtgggc ggttttacaa attttgacga
    26341 ctcagcgacc ctagatgatg gaaaatttca tgtaacgatc gtaccttcct tagatatttt
    26401 ccgatttatt ttttatttgc ctagaatcat gagaggcaaa ttctatacga tccccggcat
    26461 tacttatttc acagccaaac gcatgaaaat ccaagcgcag gaaaaatctg tgggcactcg
    26521 aacggatgga gacacgacag atgatcttcc agttgaatta tctgttgtat caaaaggatt
    26581 aacagtttat tcaccaaaga aagaagaaaa agcttagaaa tagtagttaa cagaaagttg
    26641 gaagaagagt aatggatgta ttgttacaca atcctttgtg gttttacctt ttcattgtga
    26701 atttttactt attttgtctg atggggtatg ataaacatca ggctagaact agcggctggc
    26761 gtgttcctga gaataatttg atatttgtca gcctgttagg cggtggtttg ggaggattga
    26821 tctctcgaaa agttttccat cataaaactc ggaaaaagaa gtttactata ggattcgctg
    26881 caggtgtact tgttgatttg attctcattt attttttcca ttaaggagca ggagatgtcg
    26941 tctaaaggaa cgaaaactaa aatcattctg aatctgcttt tgctgattgt gatttttgcc
    27001 attatttatt atttaatcaa tcaatcattt gcagatatct ttgccgaatt gctgtctacc
    27061 agtgttgttg ttttggcagc ggctgttata ttagggacag tttaccaaat cgctgaaggg
    27121 cgctcaatca aagaaattgc tgaacctttc gcgccgaatt ttacaacgaa agatgggttc
    27181 ttgacctcta gctatattgc gttttaccga atcgtttcgt ttggtacagg aacgctgatc
    27241 tcggagattt atttttataa caaaaaagga atgaagctat cccagggagc aggggtgaca
    27301 gcactccata tgattatgta caaactggca gtgatcactt atgcattagc tggcttggtc
    27361 atccaattct ctctttttta ttctaatggt ccatcaatga tctggtttat catcgctggt
    27421 atccttttga cgatcgtgat tgttgccttt ttattggcga tgtccgtcag cttgaatgtt
    27481 caaatatttt ttgtcagctt atcgaataag ttatttaaaa gtgagcgttt gcggaaatgg
    27541 gtggatacat gcaatcagca gatctactct ttaagggaaa cggtccaaac cattatccaa
    27601 gacaggactg cgctattgcg gatttatcta tggaatatgt taaaacttgt attttggtat
    27661 gtacttcctt atttgattct gattgaaaac catcctaaaa tagattttct actgaccatt
    27721 tcgtttatca gctttgcagt agtgctttca ggggttattc ctacgcctgc tggtattggt
    27781 tcctttgaat ttgtctattt gttattattc agaccagttg ttggaacagt tgacgcagtt
    27841 tcttcgcttt tactgtatcg tttcagttcc tttatcttgc catttctcta tggatttgtg
    27901 tatgtgattg ctgaacggcg aagagtgatc aaagaagaaa taatagaagt aaaaaaagag
    27961 aataaagaaa atcaagggta ggtcttttat aagatctacc ctttttatca aatcaaagaa
    28021 aaacgttatg gataagataa tttta
//
LOCUS       DAZUKC010000033        27779 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000033 DAZUKC010000000
VERSION     DAZUKC010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.034"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(327..500)
                     /locus_tag="ACG80T_001360"
     CDS             complement(327..500)
                     /locus_tag="ACG80T_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841020.1"
                     /translation="MSMLVRKLPLSVREFLFYLVCLIASGFKLIEHLFIFAYILAMIA
                     FLSGGWLIIYLLS"
     gene            complement(503..901)
                     /locus_tag="ACG80T_001361"
     CDS             complement(503..901)
                     /locus_tag="ACG80T_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841021.1"
                     /translation="MQLRRNKNYTTIILEDFTDQELLIVGKTKADFIKKGNASIVKAS
                     KVAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKELYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            1117..2172
                     /locus_tag="ACG80T_001362"
     CDS             1117..2172
                     /locus_tag="ACG80T_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841022.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQNI
                     LSFSLANLARLEDQSDDVLTVNLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            2192..2545
                     /locus_tag="ACG80T_001363"
     CDS             2192..2545
                     /locus_tag="ACG80T_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841023.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQEHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(2727..3014)
                     /locus_tag="ACG80T_001364"
     CDS             complement(2727..3014)
                     /locus_tag="ACG80T_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841024.1"
                     /translation="MKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAKLKS
                     DSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(3027..3845)
                     /locus_tag="ACG80T_001365"
     CDS             complement(3027..3845)
                     /locus_tag="ACG80T_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4841025.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            4083..4304
                     /gene="relB"
                     /locus_tag="ACG80T_001366"
     CDS             4083..4304
                     /gene="relB"
                     /locus_tag="ACG80T_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFS4841026.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKELMKEHEENDQSISHEDMLKELGL"
     gene            4306..4572
                     /locus_tag="ACG80T_001367"
     CDS             4306..4572
                     /locus_tag="ACG80T_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS4841027.1"
                     /translation="MSYQVRYEREAQKTLKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            4739..5224
                     /locus_tag="ACG80T_001368"
     CDS             4739..5224
                     /locus_tag="ACG80T_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841028.1"
                     /translation="MGWYGAYSSANEVIEEKKGYAKNGLEIVDTKVTKTYGIILFKDA
                     EGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFFVGDQEKMVKRQLDRIKEE
                     SNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQDGSLTRFTRLSAGDVKEV
                     A"
     gene            5715..5999
                     /locus_tag="ACG80T_001369"
     CDS             5715..5999
                     /locus_tag="ACG80T_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841029.1"
                     /translation="MLKATLQQPTHVAIAESYTINAGVVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKNSFIGKLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            6006..6251
                     /locus_tag="ACG80T_001370"
     CDS             6006..6251
                     /locus_tag="ACG80T_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841030.1"
                     /translation="MWTLEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTS
                     LECVWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            6273..6527
                     /locus_tag="ACG80T_001371"
     CDS             6273..6527
                     /locus_tag="ACG80T_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841031.1"
                     /translation="MNYTDEEQITLEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            6609..6794
                     /locus_tag="ACG80T_001372"
     CDS             6609..6794
                     /locus_tag="ACG80T_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFS4841032.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            6861..7256
                     /locus_tag="ACG80T_001373"
     CDS             6861..7256
                     /locus_tag="ACG80T_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFS4841033.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            7273..7623
                     /locus_tag="ACG80T_001374"
     CDS             7273..7623
                     /locus_tag="ACG80T_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841034.1"
                     /translation="MKATHLNYFSRTLINNLLKDTTGISVSTKHFANDLIIFEDNNLR
                     IEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKKAKLLFEFQT
                     DYHNELLAQSEILD"
     gene            7645..8019
                     /locus_tag="ACG80T_001375"
     CDS             7645..8019
                     /locus_tag="ACG80T_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841035.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMSKAQRLIHYLWKYKILFL
                     FWEATLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTFITLEENLVISDAGIERN
                     IKRVSIKITLNKPTDKKLSEAF"
     gene            complement(8327..8725)
                     /locus_tag="ACG80T_001376"
     CDS             complement(8327..8725)
                     /locus_tag="ACG80T_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFS4841036.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(8917..9684)
                     /locus_tag="ACG80T_001377"
     CDS             complement(8917..9684)
                     /locus_tag="ACG80T_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFS4841037.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LKIDFAKVRKGRNYNFAVQFNISSEFDEKPYREKKLG"
     gene            complement(9720..10880)
                     /locus_tag="ACG80T_001378"
     CDS             complement(9720..10880)
                     /locus_tag="ACG80T_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFS4841038.1"
                     /translation="MKITEKNVNPKFEPTESNQKMFDLTVTLSNQLVDSIVEMDAMPQ
                     KIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSSSSKHSRLKQVIEKLHQQSVIK
                     IQRTKTNGKTEYKVISPLEITEFNDFSDAVSIKFSSSIMPLLTNLEDHFTQYTFDQLV
                     NLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDRLKNPVFSIAELRKKFSIEKKY
                     KAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTYTDIKFLLTRTMSVPVPYKQEQ
                     RDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFLISSADLCDLKFMSRLQNKVYP
                     LYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYLEKSITSYLPYVNLYD"
     gene            11929..12732
                     /locus_tag="ACG80T_001379"
     CDS             11929..12732
                     /locus_tag="ACG80T_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS4841039.1"
                     /translation="MSQEVTENVIDNDVDLVSDQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNVFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQQETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFESFDYEKIYAKKGRKVDRLRFTFSEPESNLPSISMHNWLEED"
     gene            13339..13794
                     /locus_tag="ACG80T_001380"
     CDS             13339..13794
                     /locus_tag="ACG80T_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841040.1"
                     /translation="MDAINWHEFVNYMEKLLDDLKSFSIADAVKHGLPVSTTMKMRGL
                     KVPDEIIKLRTKTIIETAKIVDSMNLDLKGKKTTYEKDFVRFLKNVIGKYLGKKSFTE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIVEYLENKEQKKGTKKGR"
     gene            13913..14971
                     /locus_tag="ACG80T_001381"
     CDS             13913..14971
                     /locus_tag="ACG80T_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HFS4841041.1"
                     /translation="MEKDVNSASTKDQYNIIGGLLGDKDKRTRHGLRIMLATFLGVFL
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLTFARSGAEITISDVWTDKTRDVTVVKLG
                     YSGNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNESDSVTKALSQYSMNDVDNEGVISFGKKAKPVN
                     TPDNINFRINPYSDTTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKTINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            15013..16962
                     /locus_tag="ACG80T_001382"
     CDS             15013..16962
                     /locus_tag="ACG80T_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4841042.1"
                     /translation="MENSTEFFKNIQTEFNHALTLMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAKRQRQEGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TIIPRLKEYERILKSEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPEASLK
                     LSKKIPDELLEEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDIAKLEEELKSIKGQPVAVYTVTMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRKLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVNDTVSLSNTILFATTNAGAE
                     MYDSMNRYSSLSRVANQENSNEYSTELKREYRKVALEIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAHNEILKSAELVRKHLNVDLRLPPQKKHFYGQEFYADDLSMHLV
                     FDRASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSKAGGFEGKGDT
                     DAEIIAKPSIAESKENKFVFSVGGV"
     gene            16965..20654
                     /locus_tag="ACG80T_001383"
     CDS             16965..20654
                     /locus_tag="ACG80T_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3659 domain-containing protein"
                     /protein_id="HFS4841043.1"
                     /translation="MKFKKSLLQNRKIKRWVVRGLVIVGALAVTLGAPFGMNDPTSDD
                     QGNKQETVANTNVAYAESKPAEKGVWTLYARILMGDKEVNDSATKTGQMNLLGQGGIS
                     ADFPYSDISKQGNAIAGGNAGTQLASTLATYNYYGYIETVSGNAIASQASSFIGGIGR
                     TLGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGKGGSDVSNPVAKALTSLL
                     NNLGLNNQLFKAIASLSMLFFTAILGYQGMKLLSKEGVNSKGLSPVLEKWIVRIFVLF
                     VFLPVAGSLLGVYINGIQSAKEDGKLPTNVVGDYIINTRLWASTQNLAPSSDTGGNVP
                     NGKPTSEIKFVDTDYEPSKKSEMIRMINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NVNTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGNAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTTNNFDATQPWGVANNATFSTQSVALMLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFVRVWEVVLTGSLAAGAGVMAISLGVVTTSLIGLQLPIFLITFVRAFGDTAV
                     GFIPNTLLGDGVANIVRSAMVLGVGYLLVFKKGEKVNNNLINMLIGLPLEVAFGFDER
                     AKLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGRGSVKDMVAGGLGAVGAQGIAD
                     RLNNMGDSNADAKTIADKVVPETPASVPFNGGGVGGTINNTSFKDLVRQDQLRNNSQQ
                     YSDHLGRMVDEKGNFVDENGEVLKDSKFGKDAKGNFIDAKGRLVDEDGNLIDTNGKPL
                     EDNEDGNIGMDSQGRLIDDKGRLVDDEGNLVDENGRKLLNGDFGQDVNGRLVDDKGRL
                     VDEDGNLVDEFGKKLGKHSNGYNVNGDTVDEDGNIIDSRDALVADRNKELEQQQHTPI
                     AVAIPDDESDEVILDQAHQGLREEAKDKVVSDQAQEELKNKSNGEDVSNQYHQTAGNV
                     PVEGQSNELHNNFDNVADRNDTVLKEKESLRQETDKDQSKLKKTTIPTPIGSTVPKVN
                     QANDSETNTGLGMAKDNQSIREPKKTTESSKSNKSKSTSNKTGKSKEPSKAKSAVEYL
                     SKTGKNVYTSADKALAKAPKGSLAGMTYEAKNEAIKLGKGTAKVAKVTAKGATKTGKA
                     VKKSTMSVKDYIAKYSEQKPTDKKS"
     gene            21065..21403
                     /locus_tag="ACG80T_001384"
     CDS             21065..21403
                     /locus_tag="ACG80T_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841044.1"
                     /translation="MFDSIKNLVSGMTPEMKTLVQAILYLMATFFALKPGFTAMKHFG
                     DAKWGEGLKYVGAVVAIFIIPVITTVMLLTMGTKTGNDLNQKANMISALIPVVGIYLV
                     TKFPKEEQLG"
     gene            21530..21751
                     /locus_tag="ACG80T_001385"
     CDS             21530..21751
                     /locus_tag="ACG80T_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841045.1"
                     /translation="MLVHANEVLTSGFKGTDLAKIMNMNVNQFYDYRNGNKKIEKARL
                     ETLIKFEKAYLYVLDKQKRTIDRKKGVLQ"
     gene            21751..23916
                     /locus_tag="ACG80T_001386"
     CDS             21751..23916
                     /locus_tag="ACG80T_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841046.1"
                     /translation="MAKFKDITESINYVAETVPVVVLSLKPQLLEVYQKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYVVIEDVQKLSSEEFPDEEDAILDSEKYVVLLKSSLN
                     YVVDLPEKFKEKLLAGVRFMDSNGQLLVIPDDPNMFEREVEEATDFYSAVDQLEVATK
                     GLERIAETFNSSDAVEFEAVAEAETEAVPDEEEALFEDNDEATEAGNQEASIEETQTE
                     TYQQPKIDEKPESQSDKENYVYEAEKIVSESDVPKNLDADLLEKDFEQIIANKFQPVV
                     LDEVSDPVDLNEEMRSIAELKPLLFLAKESMNARISIANNKINSELNKTKEQLLLKGK
                     QMLADELLRIEEATSLSGQNQFTNTLSVAKESYEAEASKIDDLVMEKSSIIRKDYDMA
                     KQAYLEAELKKLEQRYDAEHLPEVKQQEDEFRKATEESLDQQYQEAIESIKNDALKQN
                     QEAKEVVVERIINSLKPEIEKSLTEVQEKTLEIVEKTKAENEAEFHDTKEKTTDVVVK
                     YFANKEEVEKLVAKDTNELESKLVAQQNIIEDYKNRVKEFKDLADGYKLNSQKLTEQS
                     EALREDLSRARNDLSQMAEKTKSLVVTTENQTESKRDFTSKKMATIIAVSAMACGTIL
                     GSSYFISHNHQEAQAQTETSISRVENSLGAKTASTSTTDNNYEATPTTEFKVGEDVVI
                     SIDGKTTKAEVLSIDGDKAVVKAPDGNQYKIQAHLTEKN"
     gene            23928..24158
                     /locus_tag="ACG80T_001387"
     CDS             23928..24158
                     /locus_tag="ACG80T_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFS4841047.1"
                     /translation="MREENIVYPVIENQSNTKSSQLLQEVQVVYPVNELPQTNETVSK
                     SISLIGLLIVATALTVGVYRKLKVKGSISDGR"
     gene            24148..25014
                     /locus_tag="ACG80T_001388"
     CDS             24148..25014
                     /locus_tag="ACG80T_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841048.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVDWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLRFIFIPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNTARVTMGKKKPFRGTEILKVRQDGTIMYRDGSVALMFNLDGATSLTAFP
                     EEIIAQEGKSIQYQNARDRSLAEIKITSSQPQNTERQIRNMKVQKRNAKSNKAVFDIV
                     DQQERFVSEYIDGVKTTVIQYLILKSQSQMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSEIYALK"
     gene            25032..26990
                     /locus_tag="ACG80T_001389"
     CDS             25032..26990
                     /locus_tag="ACG80T_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841049.1"
                     /translation="MELMSRSKLLNFRKKKHKKEQNADSSKAVNNTYVSDLTSLVGYQ
                     FTPSYIRTGNRWGTIVKLVNTYGMNRNYEFGWMVKAIPEVKVEGVKAYLIEADKPVSQ
                     KDQQEIMHENVGGSTNSYENNLNDKNSDAEINKTVMRIIDLNTASLLDSTANKIIDWK
                     LEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSFGGDQRDLFDRLLSEPVGSVYEY
                     TSMSSDFAGNDHAIRKGLDDADGWAIGTMSTSYASGVAFMAMDRSFKGKVLVASHKDA
                     TIKGYSEEISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGETFVAKPSLNSV
                     IEKINLAEEGLNSLELFGDRKDIGDLYRRNLDKLTQIFYLLADRELNPATRVLLKKEL
                     GNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEIDRII
                     VLKDTLRTALDSFSKMFNQRTTLKEIGKSDKLQFYYNLSDLRTEINVMEAQFLNTFDF
                     ITSQAKKGDIVMIHGFDRLSVETVGLIKKRLTMLEEDGVRLAFLFDTIGSGESKDGIE
                     TADIFNTDGKIYQSFESDFNYTIIGKMTSEELMKYEKKVAPARKGEDDQKLSVDLRQY
                     LTSGRSNKFQIRRPADLTNNFVIGEFII"
     gene            27078..27686
                     /locus_tag="ACG80T_001390"
     CDS             27078..27686
                     /locus_tag="ACG80T_001390"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4841050.1"
                     /translation="MKSNIKIIAGDKMKEKENLVWYFNRNPHMLITGRTATGKTYLLK
                     RMADYLSQVGIVSASFDKTEFEEILSSSRKEMEDRFEYIKENRFKMFDVSYSDLDMKP
                     YFVLIDELDSRLLLLEKENPLAYNYLLLIIKDIVLKGRVVGVFFIAVRQGKQPLLESI
                     EDNMLTRIFLGDSKGNGTICFDGNTKKFQTELVTEDFFSVDC"
BASE COUNT         9872 a         4349 c         5163 g         8395 t
ORIGIN      
        1 aatactggat catattaatc tgaatacaat atatgaacta ataaatagct taacctcgct
       61 attttgtttt ttctgatcat tatctacaca gcattaacaa atagaatttt aaaaataaaa
      121 aaagtaagac aaaaaaagta tcaacataaa catatacata tacacgttta tgttgataca
      181 cttaatttgt cctttaaaaa gcactgattt ttaaattgcg ttattttata acccgaatac
      241 agtctgcttg ttaggcattg tccttaaatt acatttttca taggttattt aatcatttaa
      301 tctgttgatt attcttgatc taaaacttag cttaacaaat atataatcag ccaaccgcct
      361 gataaaaatg cgatcattgc taatatataa gcaaagataa aaagatgttc gatcagctta
      421 aaccctgatg cgattaagca gaccaagtaa aatagaaatt ctcttactga taatggtaat
      481 tttctaacta gcatactcat aattaacacc ccaaagtaat attgtatttt gaacatttta
      541 catccagtac agcatcaatc aaccattgcc aatcaaaaat acaaggcttc attgaagtat
      601 gtttttcaga tgaaaagata taatataatt cctttggaat aatattttca gttgttggga
      661 tcaagctaaa tctaattcca tctaactcat agctaccagc tttctctcca ttaaaaattt
      721 ccttagcact ttcttctaaa gaatcaaaat atttttcgac agcaaccttt gaagctttta
      781 ctattgaagc atttcccttt ttgataaaat ctgcttttgt tttaccgaca ataagtaatt
      841 cttgatcagt aaaatcttct aaaataatgg tagtgtaatt cttatttctt ctcaattgca
      901 tcaggatcac tccttaatat aatttatgtt tttataatat cataataaga taaatttaca
      961 agtatttgtt tacacctttt tatttacact ttattataat taattatact tttatgattg
     1021 catttttttc ttataaacag ttgcaactaa tagtaaatag gtgtaaacta ttttcgtacc
     1081 gagataccat aaacacaaac agaaagaggt cataccatga aagaaccatt attgttttcc
     1141 gttgacttag ggaataaatc agtaaaagaa atgagtaccc acgtttctcc actctcactt
     1201 ccctctcgta ttttgaacaa aaatatcatt aattcaggac gtttttctaa tataggttcc
     1261 tcattaaaac gatcactaaa cgtttctgaa tatagcctag taaacactac tgaaagctat
     1321 tactttggga aggatataca cgagctagga aaagatgact acgtgataga atcactaggt
     1381 tttgggatta gcagatacaa gcaggaaacc taccaaaata tcctatcttt ctctttagct
     1441 aacctagcac gtttagaaga tcaatctgat gacgttttaa ccgttaatct agttttaggt
     1501 ctgccttcat ccgactatac cgaaacaatc gtcaaatacg tgatgcaaca agccacacga
     1561 caacattcag ttgaagttga cggtaaggct tataatataa acgtagatac cgtttgggta
     1621 ctgccacagc caatcggaac gctatataac ctcctacttg ctgatgatgg tagcgtacaa
     1681 gatgaaacct tgatcagtca gaagatcggt tttgttgata ttggtggtgg cacgaaactt
     1741 tttgatgttg tccaaaattt tgaaattgac gaaaacaatc gtgaacaaaa ggataccggc
     1801 atttttactt tatataatcg tatcgtaaat atgcttgacc ttccgatcag tcctaatgct
     1861 ttccaacttg aaaagattat ccgagaaggc aaagaaaaag gcgtatatct atttaagcca
     1921 agcggaaatg aaagttttga tatttcagaa gtagttgaaa gagaaataaa aacatacaca
     1981 aatatggttg ttgcagaagt tttagctaca ttcaaaaact tcacgacctt agataaagtt
     2041 gtctttactg gtggtggagc ttctattatc gacaaacaac ttgtaaaaga taaaattccg
     2101 cataccttgt ttgttgaaga cagtgaattt tctaatgtaa aaggctttta caagtttggt
     2161 ttaactcaat aaaacagcga aggagatcaa catgaataag ggaaaatcag agcgttatgt
     2221 agttataatc aatagagaaa aggttagcga gttaccttta gcagagtttc ttgaatccga
     2281 atatgaaaca ctaggcgtta aatactctca gacgatcaaa aaagctttga acctatatcg
     2341 gcgtgtaaag aacaatgaag ttacaattat tgaagcaggc aacgatagaa acgatagcaa
     2401 agtagcagaa gtaaagaata aaccaactga tcataaccct attcaacaag agcatccttc
     2461 aacggttaca tccgatcatt cagcaaagaa agaaaagcca atgctaaaag ggttcaatgc
     2521 accaagtaac aaagacttac tttaaaatga aaaaggcgag gttacaatac ctagcctttt
     2581 tattatgtac aaatataaac ataaacatat acgtatacat gtatataaac atgtatacgt
     2641 atatatccat aaacacgtat atgaaattta gttaagctat acctaaccgc ttattccaat
     2701 aaaaaaagtg atcagcttta aactgatcac tcaccaaaaa cttcatcata caagttatct
     2761 atcatttcac caagtaaagc tgaatctgat cgaattccta acttttcgct gattttagat
     2821 agcttctttc tttgcgatgg ttgaagcgtg aattgataat ttttcttctt ttcagaacta
     2881 tcacttttta gtttagctac caaagaactt gttgtttctt taggtgtttg tttaggttct
     2941 tcaactgctt caaccacttc aggaacttct tctacttcag cttcagcgaa ttttttaaat
     3001 ctattttctt tcacttcaat cactccttat tcttgtgccg tatcaattat cttgttgtat
     3061 agcgtaaacg tgctttcaaa gaaatcctta ttcttacgat agatattctt atccttagcg
     3121 aactcagcaa tattcttacc ttctaagcta gttgcattaa gcaattcctt ctcttgtaca
     3181 tatccaagat aattagataa cgttggtacc aattctagga actcacgact tgaattagta
     3241 ttatgtttga tcctgctacc gatcgtagcg tatttagttt ccatcagtgt ttcaccagtc
     3301 ttaatatcaa tcaactgttt tgctagtgca tcaatatctt taccaagctt cagcaaagct
     3361 tcaaaaccat ctttagatgg atcagaaatg cctacgacaa gatcagatac agcccaagca
     3421 ttttgagcta gtagacctgc atcattatga gtatcaatca gaattagatc atacttctct
     3481 ttaagctgtt catagttaac aacgatccat ctaaacagta gggttctgtt gttaggcata
     3541 tcacgcattt gcatctcaac ttgttttaaa tcttcacctg ctactaaaat atccatgttc
     3601 tcatttatag ctaaaggttc agctgatcca tgaagatata aatgattgat tgtattctct
     3661 cttttcgctt tactgaaagc atcaggaaag cgttttgata ggttttgcga cttatcttca
     3721 tcaatataca gcaccttcat acctaaattt tcgacagcat atccacctag attgtagatt
     3781 gttgttgttt tacctgttcc acctttttca atgtttccag taatgattga caacttcttt
     3841 tccatgatta aacctccctt attaaccttt tgtaactcca ttatacacta taaacacctt
     3901 tatgtatatg tttatataca taaaggtgtt tatatttata tttgctttta tattctataa
     3961 cataaacata tatatgtata tgtttatgta tatgtttatg tttatgtata tgttcatata
     4021 tataactatt gcatctgtca tacaatcgta gtacaatgta attgaaaagg agttgataaa
     4081 ctatgccagt aataagtatt cgtgtatcta aagaagaaaa agattttctt gaaaaagttg
     4141 ctgaattcaa cggagataac atttctgaat ttatgcgtag taattccctg caaacagcag
     4201 aaacgttagt agactttgct acctacaagg aactaatgaa agagcatgag gaaaacgatc
     4261 aaagtatttc ccacgaagat atgctaaaag agttaggctt ataagatgag ttaccaagta
     4321 agatatgaac gtgaagctca aaaaacgcta aaaaaaatgg ataagtttca ggcaaaggtt
     4381 atcctaaact ggatcgagaa aaatcttgta ggcactgatg atccaagaaa gcatggtaaa
     4441 ggtctaacag ctaataaatc aggctactgg cgatataggg taggtgcata ccgtataata
     4501 gctgatatta gcgagcatga agtgacaatt cttatattgt ctattggtca cagaagtgaa
     4561 gtttataagt aacagacaag ctgataaggc ttgtctgttt ttttgttaac ccaacaaaat
     4621 cactgtttac gatcccactt tacctaaaaa agaacaatat cctcttgtta ttatacacac
     4681 cagtgtgtat aatataagtg taaacaagct aattaacttt caaaaggagc gattttttat
     4741 gggatggtat ggtgcttatt catcagcaaa tgaagtaatc gaagaaaaga aagggtacgc
     4801 caaaaatggt ttggaaatcg tagataccaa agtaacgaag acctatggaa ttatcctttt
     4861 caaagatgca gaaggaacct acgatattga cagcttcatc tttcgagatg aaatgtacaa
     4921 accaatctac ttcttaaatg acagtcctgc ttactttaag aaaattccaa aaaaatggtt
     4981 gaaattcttt gtcggtgatc aagaaaaaat ggttaagcga caactagata gaattaaaga
     5041 agaaagcaat aaaccaaaac ttgatgactt attagttgca ggtaaaactt actcaatctg
     5101 ggatggatcg tatgtcgctg tttatcagta taaactcaaa cgtagcttta ttttccgact
     5161 acaagatgga tcactcactc ggttcactcg actatcagca ggagatgtta aggaagtagc
     5221 ctaaaactta aatacataaa cataaacgtg tatacgttta tgtttatgta tatgtatatg
     5281 aatataaacg tattaggtac gaacgctttt aaatgctttt ctgggctttg attttagggt
     5341 agggctaaaa catcaccatc tgatccaaaa cagctcagaa acgcatttaa acgtatctga
     5401 taccaatatt acagaaggag tgtatcgtat gattaaccta tttcttttag tgacagctag
     5461 tacattattc tttactggaa tatcacttat aatttcctat ttgatctata agaaagccga
     5521 aacttacaca agcaaagcaa ccggactagc cgtttcatcc tttttaactt tgctgacttt
     5581 ttttgttact ctatcgtttt cttttgttta gcacagctga tccatcagca aaaaacctct
     5641 ctttacatta tacacactag tgtgtataat gtaattgtaa acaagctaat taacttttag
     5701 gaaggaatga caatatgcta aaagctacat tacagcaacc tactcacgtt gctattgcag
     5761 aaagctatac aattaatgca ggggtcgtta aacagctgat caaggatcga gaattcaaaa
     5821 agtttaacag tcgtcaagcg tataacagac agttaaagaa agctattcta aagcacaaaa
     5881 attccttcat cgggaaattg gaaagtgtgg atgttaaaac tgtttcaacg gcattttcag
     5941 ttgttgaagt gactattgac ggtcgcttga tttctctatt tgcttttgga attaattgat
     6001 agggagtgtg gactttggaa ggtaagactt ttgcagaaat ttggactgaa tttattgagg
     6061 taccaactcg cattgatccg ataacttctg aaaaggtatt ggatgcagac ttctacaatc
     6121 tcaaagcagg tactagcctt gaatgcgttt ggatgtattt agaagagcat ttcaaaggcg
     6181 taacgctcgg tcacgtctta taccacaatg gtgccgagtg ggatgcaatt ggaaatatcc
     6241 atttttctta atacaagaaa ggagaacaca atatgaatta taccgatgaa gaacaaatca
     6301 ctttggaata cgttttcaca aatgaagaag gggaggaaat tgaaaaagtc ttgatcattt
     6361 catcaaaaga cttggagaag gctttggctt atgaaccata cgaatcaacc gtaactgtca
     6421 ttgaattctt agatacctat acttctaatg atacttggga aattatgaat atttgcaacg
     6481 ctaataacat tcaatttgct gaaaaaacca ataaatcatt ttattagttt tgcaataaaa
     6541 catcctcttt ttctattgat tccatacaca acaatgtgta taatagattt tgtaaaggga
     6601 gggtagctat gcctaagaca gcaagagaaa ttgtaaaaat tctcaaatct aacggcttca
     6661 aaaagactgg acaaactggc agtcatgcaa aattttataa ttccaagact aaaagaacgg
     6721 tcgttgttcc gatccacaat ggtgatgtac cactaggaac agaagctaat atctggaagc
     6781 aagcagggct ttaacagcct tgccttccta tttggtatta ggcatactat cctctcttac
     6841 actagaaaag gagcgaggaa atgaagacaa aaaatatttt ttatccggca atcttcgaga
     6901 aagaaggaga agcttataac atcgttttcc ctgacttacc ggaaatatct acctttggaa
     6961 atagtcttga agaagcatat ttaaacgcta aggatgcttt aggattagcg ctatatagtg
     7021 taccagatgc agaatttcct aaaccatcag cagtggaaga attaactttg aaagccaacc
     7081 aagtggtagt aatcatccaa ctaaacatta aacttttccg aagaagctta aatacaaaaa
     7141 caattagaaa aaatgttagt gttccggaat ggttagttct tttagggaag gagaaaaaca
     7201 taaacttctc tcaactactt caaaaagcac tagaagaaga gttgcttgaa aagtaaaaag
     7261 gagaccgaac ctatgaaagc tacacactta aactatttct ctcgaacgtt aatcaataat
     7321 ctattaaaag atacaactgg cattagcgtt agtaccaaac actttgccaa tgatctaatt
     7381 atttttgagg ataataactt gcgaattgag atgtttattg aacaggcaac ccctaactca
     7441 atacctgaat tctgctatac gcacactgaa aaaatgaaac atgcaaaatc agttaaagat
     7501 gttgaagcat taagtgaatc actaacgaac gctttaaacc tcgttaagaa ggcaaaattg
     7561 ttatttgaat ttcaaaccga ctaccacaat gagttgcttg ctcaatctga aattttagat
     7621 taattgaaac aggagaagag aaagatgaaa caaacacaaa cagaaaccgt taacgatacg
     7681 cttgctgatc aattagcagg ttattataaa ttatttgaga tgagtaaagc acagcgcttg
     7741 atccactatc tttggaaata caaaattttg tttctttttt gggaagcaac actaacattc
     7801 tcagttcttt tttataagtc agatttttat aatcaacact tagttttagg gagttttttg
     7861 cttatctaca ccattttgat gcttaattta tataccttca tcacacttga agaaaactta
     7921 gtaattagcg atgcagggat cgagagaaac atcaaaaggg tatctatcaa gatcacgtta
     7981 aacaagccta ccgataaaaa gctctcagaa gcgttctgag agcttttgac atttgcttgt
     8041 ataaaattta tcctaacaca ttacaaagca cctaattcga gcttatatca agcaaatttg
     8101 ctatttatta ctcaatacat atctaaaaat tacttgatgg aaaactagat acatggtaag
     8161 catgaaaaga aaatcaattt gagcagatgc agtataatac atagcaattt aaaagctctc
     8221 agaagcgttc tgagagcttt taatatattc tttgataatt tgtctttctt aaatccatac
     8281 gtcctaaaac aagctgttat tgcttaatat gatcatttga gagacatcaa atcaactttt
     8341 ctctcaacaa atcattcaaa gctttcgaca gattaatatt ctcttcagcc gctaaaacat
     8401 ttaaatagtg ttccacagtc actgattttc gatcgtaatt ttgcttgttt cgagctttag
     8461 caactttcgt attagctttt acgacaaata aattaacatt ctcagatcgc ttgattcttg
     8521 catttaactc ttgaacaggc gtaggcgatg gaatttcttt ttcttgcaac tctaaaagca
     8581 ccaagtatcc ttccaatact tcttgcgcca tctcaaaagc atcttctaaa gtttcaccat
     8641 gcgtatataa gtctggaaaa tctgggaatt taactgcata ttcatttcct ttccattcga
     8701 atactgcaca aaaaaagtat ttcatcgcat tgcacctctc taattaaaaa ataatttttg
     8761 ctttttagtt tcaaaaaaaa gaatcgccta ccaaaaaaat tgataggcga tcacaagcta
     8821 attaacctct aaaggagatt aataccttgc tcccaaggca ctacatatct tagcatacgt
     8881 ctgaagcatc ttcaaacgag taagatatat tttgcattaa cctaattttt tttctctata
     8941 aggtttttca tcaaactcac tagaaatatt aaactggaca gcaaaattgt aatttctgcc
     9001 ttttctaact ttagcaaaat caatcttcag cattgatttt tcattaattt cctcaactga
     9061 ttcccttaaa acacgtctat caaaattcga tattgtttta tactcatttt ctgttttcgt
     9121 aatctcacga attttaacta tttcaatcag gggattctta tatatttcag ctttctcaaa
     9181 atctccttta tttttggcga taagatattt tttataatac atcgccaaat atttgtataa
     9241 cacgatgcta tatttttttt gaaaagatgc taagtcttca aaaacaaaga acggatactt
     9301 ctttttgatt gagacaatta agggaattac atcttctgaa aaactgaaag atacactatg
     9361 ctcttctaaa tcccaatgaa tccgatcaat agcagataca gttgtatacg tcttatattt
     9421 cttgtctaga ttcgatattt cataaactgc ttgtctttgc aaagtgttaa ctatggtttt
     9481 tagttcagtt tcaagatttt gatcaacatc catgtaatta ataatcgctt taacaggtaa
     9541 atctactacg cctgttttct ttagctttgt gggcgagata taactgaaca gcaattgata
     9601 aagttcatac gactttaaat ccattttcat gtcaattgtt gctaagaact cctcgttgta
     9661 ctttgtcagt tcatcttttt tcaaaagctt ttccttcctt cacgcaagtt tttcagttgt
     9721 taatcataaa ggttcacata aggtaaataa ctcgtaattg atttttcgag atatttaaca
     9781 gtatttttta ttgtgtaatc ctccttatga gtagcaacgt aagatagatg attgacaact
     9841 gattcaaatc ctctatcttt ggataaacga tcataaagcg gataaacttt attctgaagc
     9901 cgactcataa atttcagatc gcataagtcg gcagaactga tcaaaaagtt tttgattagt
     9961 aatttagtgt atttgctatt catagctttc aagtacgatt gattcatttt ctctgtttta
    10021 tcttctgcgc cttttaaata agcagggtct ctttgttctt gcttataagg taccggtact
    10081 gacatggtac gtgtaagcaa gaactttata tctgtataag tatgaccctt cttgataagc
    10141 tcatagtcaa ttttaaaatc agtgtattta ttcagttggg caatcggttc tttaattaca
    10201 cgtctttcaa agtctcgaaa agctttatat tttttctcaa tactgaattt ctttcgtaac
    10261 tctgcaattg aaaaaacggg gttttttaat ctatcaagtg attttgtact ccatgttaag
    10321 ttatctctat ttgcttcata cagattaaaa tctttagtga ataattgaaa aaaactgatc
    10381 gcatatttgc ttgtcaagtt aactaattga tcaaatgtat attgtgtaaa atgatcctct
    10441 aagttcgtta acaaaggcat aattgaacta ctaaatttaa tagatactgc atcagaaaaa
    10501 tcattgaatt cagtaatttc tagcggtgag atgactttgt attcagtctt accgttagtt
    10561 ttagttcttt gaattttaat gacagattgc tgatgcaatt tttcaataac ttgttttaat
    10621 cttgaatgtt ttgaagaaga tgaactagaa gacatcaacg caaataaagc ctttttagat
    10681 actttgacgg tattattaaa aggcggattt ttagggtcaa gatgtcctat agcgtaaaga
    10741 taaatttttt gtggcatagc atccatttca acaatgctat ctacaagttg attcgataaa
    10801 gtaacagtta aatcaaacat tttttgattg ctttcggtag gttcaaattt cgggtttaca
    10861 ttcttttctg ttattttcat acgattgcct cttctttcta agcttggtac gagcttaaaa
    10921 acttggaact ggtacgattc cattgaataa gtatagacct gttttcgata cgatacaata
    10981 agaaatgtat cgtaaattta taacataact caatctcagt aaaacgcatt ttatttttat
    11041 taatcaaatt tcattgataa gacagcagaa atcgctattt tagaaccttt acaaaataaa
    11101 aatccttttc aaaaaaaata cgtaattata aaaattcaca aaattaaaaa acaaatattt
    11161 ttttagtttt tagaaataaa ttcacatact ttgtcttttt ttgtatatat tcggaatacc
    11221 acatcgcttt ttgtccatgt ttgcatcgct ttttgtccac attcacatcg ctttttgtcc
    11281 atgttcgcat cgctttatgt ccaccctaaa atctatttaa gattgatata aagccttttt
    11341 ggaggttgct aaagttttaa agattaaaat atatattata aatataaata aaaggggaag
    11401 ctgaagcttc ctccttttcc tttttttcaa ttattcaaaa ttacacaagc aaaataaatt
    11461 gctagatagt gttagttttt gttttaaggt ttttaatttt tgagattttg aaataagaga
    11521 ggaggaatcc tcctttatta aatatctgtt aatatatctg ttaaatatct gttaatatat
    11581 ctgttagggg ggtcttaaac ccttgggaga gtaagtattg aaggggtatg aaggtaacac
    11641 ttttggacag taaaggtaac aaaagtggac agtaagggta acatttttgg acagtaaagg
    11701 taacaaaagt ggacatttga ggtatcaaat caggataaga cgttaccttc ggttttcttg
    11761 atttggaaat ctaaaaagta gaaaactaga ttagtaacaa atctttgttg actgttacct
    11821 ttagttgtga tttaatttag gtaacaagca gaaaaaaagc gtttccttta atttagtacc
    11881 aaattaacat gattaaaggg atgcaacaag aagattggag agtaatcaat gtctcaagaa
    11941 gttaccgaaa acgttattga taatgatgta gatttagtat cagatcaaac aaatgcaatt
    12001 gttcgttata acaatggttt gaacacaaca gctttgcgag gatttaaacc tgtagaaatg
    12061 gatgtgtttt gggctatttt atcaaaaatg aaacgtcaag gaacagcaga agtaactttt
    12121 gatttcaacg tctttcgtta tttggtcaaa tacgacagaa gagaaaagga cagtttttat
    12181 tctgtgttaa aagctttgag tgacaaacta ggtactttaa cctaccgctt tgaagattca
    12241 gatgtctatg aacagctgtg gctttttcaa aaattccgaa tagataaaca gcaagaaaca
    12301 gttacaatca aggcaaatga ggagtttgaa tttatcttaa attcaattgg caaaaacttt
    12361 actcgttttg aacttgataa ctttacaaaa ctaagaagta gctacacaaa agagctgtat
    12421 cgtcatctca tgcaatatag aaataaagag tggcgaaatg gctattgggt agtcaaagtt
    12481 gaagatttta gaagagcgtt aagtattcct tcaagttaca gaatgacaaa tattgacaaa
    12541 agaatattag agcaggcgaa agaagaattt ttagctcctg atgaaaatgg tatacaggta
    12601 tttgaatctt ttgattatga aaaaatctat gcgaagaaag gaagaaaagt tgatcggctc
    12661 agattcactt ttagtgaacc agaatcaaac ttgccatcca tttcaatgca taattggtta
    12721 gaagaagact gaaaagaagc aggttgtaat tttaatacaa cctgcttctt ttgaaaaaag
    12781 tatgggtaac acttttggac aataaatgca agaagttttt ttgtgaccaa actttatatt
    12841 agattactgt gattttgtgt gttagaaaag ccctgataga aaagcaaatt caaacacttt
    12901 ttaatatgta tgggtaacac ttttggacat gtcaataata aatatatttt ctttcgtctt
    12961 ttgcctaact aaaggtaaca cttttggaca gtatagtttt gtaaactaat tagatcgggt
    13021 ttaatgggat atatcttagc atttatgtaa gaatgaaggt aacattaatg gacatgtcaa
    13081 ctgtttgaac aaaaaaatat agaatatttg gatctgattt ttcatttttc caaacgttaa
    13141 aaaatagtta gttattttat taatagatag tcatccttaa tgtttgaaat agcagaaaat
    13201 aagatattgt ttgataaatg ttgttgttat aaggaaaact tgaatttgtt aattgattta
    13261 tttatgttat tatgatacta taataacata aatgtgttac gcccgaacct cattttacaa
    13321 aaatgaaggg gtgagacaat ggatgctatt aattggcatg aatttgttaa ctatatggag
    13381 aaactactag atgatctgaa aagttttagc attgctgatg ccgtaaaaca tgggttgcca
    13441 gttagtacaa cgatgaaaat gcgagggttg aaggttccgg atgaaattat caagctgagg
    13501 actaaaacaa tcattgaaac tgcaaaaatt gttgacagta tgaaccttga tttgaaaggc
    13561 aaaaaaacaa cttatgagaa agactttgta cgttttttaa aaaatgttat cggaaaatat
    13621 ctagggaaaa agtcttttac tgaattgaga gacgaagaga ttgtaccaac tccaatggtt
    13681 agcaaaattc gcaaaaacaa agccgaggga aattactatc ttggcacaat agcaaaaatc
    13741 gttgagtatt tggaaaataa agagcaaaag aaaggaacga agaaaggaag atagttcgtg
    13801 tgataaatat gaatagggaa gaatgtaccg aaatgataaa aaaacccgaa cctagaatta
    13861 ccgaaaaact gactgattgc ttgctctttt acgaaagggg gcaggcaaac taatggaaaa
    13921 agatgtcaat tcagcatcca ctaaagatca atacaatatc attggaggat tacttggtga
    13981 taaggataag agaacacgtc atggattacg cattatgctt gcgacatttc taggggtgtt
    14041 tcttttatta acaggcttgg gtagctacat ttatcaatta ggaaactata attattatct
    14101 tgtcaacaat acaacaccaa tcggctcatc attgacgttt gctcgatcag gagcagagat
    14161 cactatctca gatgtttgga cagataaaac tagagatgta acggtggtca aacttggtta
    14221 ctcaggaaac gcaagaaaat tgctatctac acaaggtaaa aattataact tgtatatggc
    14281 aacaaaagaa aaagatcgtc ctaagaagct tgaaatgagt tatgggatgt taagtaccga
    14341 tggtgatgct ttcttgttcc taaaaggtaa attagatgaa agagcttatc aagtttttat
    14401 tgctaaccaa gtatcactaa tttcaggtga tgatacagct gatgatgata ccaaagtcaa
    14461 tgtgaatgaa tcagattcag tcaccaaggc actttctcaa tattctatga atgatgttga
    14521 taatgaagga gtaatttctt ttgggaaaaa agcaaaacca gttaatactc ctgataatat
    14581 caatttcaga ataaatccat actcagatac aactaacgta tatcaaggaa gcttccttac
    14641 caaagatggc gatattgatt actcaaaagt tgtagctgtg acaagtaaga ataatgttgt
    14701 aaataagcta gaagaccagt taaatgcttc aaatactaac atcaagaaaa tcgaagccac
    14761 aaaagctgag tatcttgaac gtttacaaca aaatcctgac gataaagatt cgcaagaaag
    14821 tattcgtcaa ctagacagtc gaatggagac cgaaattaaa acaatcaatg atacacaaaa
    14881 attgattgat cagtacaaaa atgctgactt taacaaagat tcttttggag atatgaagac
    14941 aacatttaag tacctaaccc tgactgacta gaaacaaatt attcttattt tagaggaagt
    15001 gaacacaaac agatggaaaa tagtaccgag ttctttaaaa atattcaaac cgaattcaat
    15061 catgctctca cactaatgaa accaccgaaa cataagttag tggggcgtga tgacgaactc
    15121 catgagttgt cactgatctt aaatagaccg gaaacaccac aagctatctt gttaggtgaa
    15181 gcaggaacag gtaaaacagc actggcaagt gaatgggcta aaagacagcg acaagagggg
    15241 aagaaagtac aagtatataa attatcaatc ggacaattgc gagttggagg agatgacgtc
    15301 ctccaaacac gtttggaaac tataattcca agattgaagg aatacgaacg aattttaaaa
    15361 tccgaggaca aagataatga atttgtcctc tttatagatg aatttcataa agttgtcagt
    15421 tcatttggga gcggtaataa aattggggga gattcgctga aggaaccttt agcaaatgaa
    15481 tcacttcagt tgataaccgc tacaactaga aaagaatatg aaaactatat caaacgagat
    15541 gatgctttag gtagacgact acaaccgtta gaactcaacg aattaaaaag tgatgtcgtt
    15601 gtcgatatac taagacaatg gttgaataca tcatctgtca aagatgatcc tgaagcttcc
    15661 ttgaaattat ccaaaaagat tcctgatgaa ttgcttgaag aaatcgtaca agcaaacaag
    15721 ctttatcgtg aggatttatc cgaaccggct aaatcaattg ccgtaatgga agatttgatt
    15781 gcgaaggatc gtgtagatgg caatgcgtat aatcgagaaa gcctgttaca aatatttgga
    15841 aaatataata ttcaactaga ttctgatttt gatatagcaa aattggaaga ggagctaaag
    15901 tccattaaag ggcaaccagt agcggtatat acggtcacga tgttgtttaa acgaatgaaa
    15961 cttggatttg atcgttttaa agggcgaaag caaccaaaag caacggcttt gtttacagga
    16021 tcaacagggg tcggtaaaac tgcgatggct aaagctgtag caagagggct atacggtgat
    16081 gaaaataaag tccttttagt aaatatgcca gactataaaa ctaaagatga tgctatgcgg
    16141 tttagaaagc ttatcggaac acatgtccgg catgatccat cagcaatcat tgtttgtgat
    16201 gaatatgaaa aagcaggcgt agaaacactt gatgcctttt tatctattct agatgaagga
    16261 agaatcagtt ttactgaaag aggatgggat gatcgagaag ttaatgatac cgtctcactt
    16321 tcaaatacga ttttatttgc gacaacgaac gcaggggctg agatgtacga ttcgatgaac
    16381 cgatacagct cactatctcg tgttgctaat caggaaaatt caaatgagta cagcactgag
    16441 cttaaaagag agtatcgaaa ggtagcatta gagattaaac aagctctaat tggtgaaggt
    16501 ctaaaacccg aagtcatcaa ccgatttgga cggattgttc cattttgggc tttatcggaa
    16561 caaactcttt tggaaattgc acacaatgaa atattgaaaa gtgctgaatt ggtaagaaaa
    16621 cacttaaacg tagacttacg ccttccacca cagaaaaaac atttctatgg tcaagaattt
    16681 tatgcagatg atctctccat gcacttagtt tttgatagag cttcacaaga agattctaaa
    16741 tcagggggtg caagaaatat cttgcatctg gtcgaatcag aatttgaaga agcaattact
    16801 gaagcaataa ttacctatcc taacgccaaa atatttgacg ttaaattgag caaagcagga
    16861 gggtttgaag ggaaaggcga tacggatgca gaaattatcg ctaaaccttc catcgctgaa
    16921 agtaaagaaa acaagtttgt gttttcggtt ggaggggtgt agctatgaaa tttaaaaagt
    16981 ctttacttca aaatcgtaaa attaagcgat gggttgtaag aggtttagtt atcgtggggg
    17041 cacttgcagt tactttaggc gctccatttg gaatgaatga tccaacgtct gatgatcaag
    17101 gaaataaaca agaaacagtt gcaaatacga atgtggctta tgctgaaagt aaaccggcag
    17161 aaaaaggggt atggacacta tatgctcgta tcctgatggg agataaagag gtcaatgata
    17221 gtgccactaa aactggtcaa atgaatttac ttggtcaagg aggtattagt gctgactttc
    17281 cttattctga tatttccaaa caaggtaatg caattgccgg tggtaacgca ggtacgcaat
    17341 tagcatcaac cttagcgact tataattact atgggtatat cgaaacagtt tcaggaaatg
    17401 cgattgcaag tcaggcatcc tcttttattg gtggtatcgg tagaacgctt ggcagtttta
    17461 tcggatcaat tagcttgata ctttctaata ttgctgaatc catcaatgaa ctccttggaa
    17521 aatttttcat gagtatgaat atattttcac ttatgggttg gggtaaaggt ggatcggatg
    17581 tttctaatcc ggtagctaag gcgttaacta gcttacttaa taatttggga ttaaacaatc
    17641 aattatttaa agcgatagca agcttatcta tgctcttttt tacagcaata ctaggctatc
    17701 aaggaatgaa actgttgtcg aaagagggtg ttaactcaaa gggattatca ccagtacttg
    17761 aaaaatggat tgttcgtatt tttgtattgt ttgtttttct accagttgca ggatcgttat
    17821 tgggagttta tatcaatggt attcagtcag caaaagaaga tggaaaatta ccaacaaatg
    17881 ttgttggtga ttacatcatt aatacaagac tttgggcttc tactcaaaat cttgctccat
    17941 catccgatac tggtgggaat gtgccaaacg gaaaaccaac atctgaaatt aaatttgttg
    18001 acactgacta tgaaccatct aaaaaatcag aaatgatcag aatgatcaat actagcagtt
    18061 acgctagaca aaataatgat ctagattcca attcagtcgg catggcattg ttaaaagaat
    18121 ggtcaagtaa tgatacgttt aatgtcaata cttatgccgg tgatgtgaat gtagctgaaa
    18181 atgatgctta tatgggaaga gcgcctgctg tttggacagg ctataattta ggggatggaa
    18241 aagaaggaaa tgcaattaag aaacctacca atttaaatgg ttatatttgg tcagctaatc
    18301 aaaacgttac agacgacaac cgcaagcctg atggaaaagg aacaacgaat aactttgatg
    18361 ctactcaacc ttggggtgtt gctaacaatg caacgtttag cacacagtcg gtagcgttga
    18421 tgctccaatc agcactggat acatcagggg ctaagtttta tgcgtataat atcgctccat
    18481 caggaattca aggtagtgct aagaatatgt caacagtaaa gacagaatgg cgtacagctt
    18541 ctttagttgg tcatggagct atgggtaaat tcggctcatg gttagcgatg aacgctgaat
    18601 caatcgtatt agcagtgctt tatctagctt gtgcttgggc gatgtttaac gttaatattg
    18661 taaaagcctt caaacgtgag tttgtgcgag tttgggaagt agtcttaaca gggtctcttg
    18721 ccgcaggagc aggggttatg gcaatctcat taggagtcgt aaccacatct cttatcggat
    18781 tacaattacc tatttttctg atcacctttg ttcgtgcatt tggagatacg gctgtaggat
    18841 tcatccctaa cacattactt ggtgatggtg tagcaaatat tgtacgatcc gctatggtct
    18901 taggcgtagg ataccttcta gtctttaaga aaggtgaaaa ggttaataat aatttgatta
    18961 atatgcttat tggattacca cttgaagtag cgtttggttt tgacgaacgt gctaagttat
    19021 tatttggcaa tagaaatgat gctgttcgag ttgtggatgg cgtttctaaa gcaatcaaaa
    19081 aaagccaagg ggctaaaaat ccttattatg atggtggcag aggatcagta aaagatatgg
    19141 tcgcaggagg acttggagca gtcggagcgc aaggaattgc tgatcgtttg aacaatatgg
    19201 gagacagcaa tgctgatgct aagacaatcg ctgataaagt tgttcctgaa acgcctgctt
    19261 cagtaccatt taatggtggc ggagttggtg gaacaatcaa taatacctct ttcaaagatt
    19321 tagttcgtca agatcaatta cgtaataact cgcaacaata tagcgatcat ctaggacgga
    19381 tggttgatga aaaaggaaac tttgtcgatg aaaatggtga agtattaaaa gacagtaaat
    19441 ttggtaaaga tgccaaagga aactttattg atgccaaagg gcgtttggtc gatgaagatg
    19501 gaaatttgat agatacaaac ggtaaaccat tggaagacaa tgaagatgga aacatcggta
    19561 tggatagcca aggacgttta attgatgaca aagggcgctt ggtcgatgac gaaggaaacc
    19621 ttgttgatga aaacggacgt aaattactta acggtgattt tgggcaggat gtcaatggtc
    19681 gtctagttga tgataaagga cgattagttg atgaagacgg aaatctggtc gatgagtttg
    19741 gtaagaaact tggaaaacac agtaacggtt ataacgtaaa tggcgataca gtcgatgaag
    19801 atggaaacat cattgattca agagatgctc ttgtagcaga tcgaaataaa gaactcgaac
    19861 aacaacagca tacaccaatt gcggttgcaa tccctgatga tgaaagcgat gaagttattt
    19921 tagatcaagc acatcaaggg ttaagagaag aagcaaagga taaagttgtt tcagatcaag
    19981 cacaagaaga gctaaaaaat aaatcgaatg gtgaagatgt ttcaaaccaa tatcatcaaa
    20041 cggctggaaa tgtacctgtt gaaggtcagt ctaatgagct tcacaacaac tttgataatg
    20101 ttgctgatcg gaatgataca gtcttaaaag aaaaagaatc attaagacaa gaaacagata
    20161 aagaccaatc aaaattgaag aaaacaacta ttcctactcc tattggttca acagttccga
    20221 aggtcaatca agctaacgat tctgaaacaa acactgggtt aggaatggcg aaagacaatc
    20281 aatccattag agaacctaaa aagacgactg aaagtagcaa gtcgaacaag tctaaatcta
    20341 cttcaaataa gacagggaaa tctaaggaac catctaaagc taaatcagca gtagaatatc
    20401 tatctaaaac tggtaagaac gtgtatactt cagcagataa agctttagca aaagcgccaa
    20461 aaggaagctt agcaggaatg acctatgaag ctaagaacga agctatcaaa ttaggtaaag
    20521 gaactgctaa agttgccaaa gtaactgcta aaggagcaac taaaactggt aaagcagtga
    20581 agaaatctac gatgtcggtg aaagattata ttgcaaaata tagcgaacaa aaaccaaccg
    20641 ataaaaagtc ttaagaagaa taaaaagagg gagcattaat atgctccttc ttttttgttc
    20701 ttctcttcaa ggggaatgtc aagaaaaact taattgtctt caaaaatttc ttttttattt
    20761 ggtagtaaat ctatgaaata tttgcttttt tagttatcgg ttaataagag ggcttatgct
    20821 agttagttaa aatcgggtaa ctagtcatta aatgctgata aataagcaat ttttacgaaa
    20881 aaaaaagaaa aaaatttgag ggaaaatacc tttcaatcta caaaaattta tgttattatg
    20941 ataacataaa gatgtaaata tcgaagcgtc ttttctgtat tccgatcatt gacaagtgaa
    21001 cagagaaaag tttgcgatga tataattatc ttgtgaaaaa taatcgaaag gaaggattta
    21061 taatatgttt gattctatta agaatttggt ttccggaatg acgccagaaa tgaaaacgtt
    21121 ggttcaagct attctatatt taatggctac attttttgca ttgaaaccag gattcactgc
    21181 tatgaaacat ttcggtgatg caaaatgggg agaaggctta aaatatgttg gagccgttgt
    21241 tgctattttt attatccctg taattacaac tgttatgttg ttaacgatgg gtactaaaac
    21301 aggtaatgac ttaaaccaaa aagctaatat gatctcagct cttattcctg tggttggtat
    21361 ttatctagtt acaaagtttc caaaagaaga acaattaggt taaaaggtaa attgagcaaa
    21421 tttggaggat atgccgattt gttcaattta ctttttgcta aaaaactaca ctcgaagcgt
    21481 agtttttgaa gtgagggaag acaatagatg taagatggac agagaaaaga tgttagttca
    21541 cgcaaacgaa gtattgacga gcggttttaa aggtaccgat ttggcgaaga taatgaatat
    21601 gaacgtaaac cagttctatg attatcgaaa cggcaataaa aagattgaaa aagccagact
    21661 tgaaacgctc atcaaatttg aaaaagcgta tctatacgtg ttagacaaac agaaaagaac
    21721 tattgaccga aaaaaaggag ttttacaata atggcaaaat tcaaagatat taccgaatct
    21781 attaattatg tagcagaaac agttcccgta gttgtactct ctttgaagcc acaattactc
    21841 gaagtgtacc aaaaagaaga aaaagaagcg aaggcaaaaa aagctaaagc aaaggtatgg
    21901 aaagaattcg atgcgttgct gaccaaagat ttaccaaaga agaattacgt ggtgattgag
    21961 gacgtacaaa agctcagcag tgaggaattt ccggatgagg aagatgcgat tctagattcc
    22021 gaaaagtacg ttgttttatt aaaatcaagt ctgaattatg ttgttgattt accggaaaaa
    22081 ttcaaagaaa agctattagc tggtgtccgc tttatggata gtaatggtca attattagtt
    22141 attcctgatg atcctaatat gtttgaaaga gaagttgaag aagctacaga cttctacagt
    22201 gcagtagatc aattagaggt agctactaaa ggcttagaaa gaattgctga gaccttcaac
    22261 tcttctgatg cggttgaatt tgaagctgta gcagaagccg aaacagaagc cgtaccagat
    22321 gaagaagaag ctctttttga ggataatgac gaagcaactg aagcaggaaa ccaagaagca
    22381 agcatagaag aaacacaaac agaaacctac caacaaccaa aaattgatga aaaacccgaa
    22441 tcccagagtg ataaagaaaa ttatgtttat gaagctgaaa aaatcgtatc tgaaagtgat
    22501 gtaccgaaaa atttagatgc tgatttgtta gaaaaagatt ttgaacagat catagccaat
    22561 aaatttcaac ctgtagtttt agatgaggtt agtgatcctg ttgatttgaa cgaagagatg
    22621 cgatccattg cagagttaaa accattgctg tttttagcta aagagagcat gaacgctcgt
    22681 atctctattg ctaataataa aatcaacagt gaattaaata aaacgaaaga gcaattgcta
    22741 ctcaaaggaa agcaaatgtt agcggatgag cttttgcgga tagaggaagc aacatcacta
    22801 agtggtcaaa atcagtttac aaatacgcta tcggtcgcta aagaaagcta cgaagcagaa
    22861 gcatctaaaa ttgatgattt agtgatggaa aaatccagta tcattcgtaa agattatgat
    22921 atggcgaaac aggcttattt ggaagcagaa ctaaagaaac tagagcaacg gtacgatgct
    22981 gaacatctgc cagaagttaa acaacaagaa gatgaattta gaaaagcaac tgaagaaagt
    23041 cttgatcaac aataccaaga agcaattgaa tctattaaaa acgatgcttt gaaacaaaat
    23101 caagaagcaa aagaagttgt ggtggaacgg atcatcaatt cattgaaacc ggaaattgaa
    23161 aaatctttaa cagaagtgca agaaaaaacg ttagagattg tcgaaaaaac aaaggcagaa
    23221 aatgaagctg agttccacga tacaaaagag aaaacaacag atgtggttgt caaatatttt
    23281 gcgaacaaag aagaagttga aaagctagta gctaaagata cgaatgagtt agaatccaaa
    23341 cttgttgcac agcaaaacat tattgaagat tataaaaatc gagtaaaaga gttcaaggat
    23401 ttagcagatg gctataaatt aaatagccaa aaattgactg aacaaagtga agctttacga
    23461 gaagatttat ctcgtgcaag aaacgaccta tcacaaatgg cggaaaaaac aaagtcacta
    23521 gtagttacta cagaaaatca aactgagtct aaacgtgatt ttacaagcaa aaagatggcg
    23581 actatcatcg ctgtatcagc aatggcttgt ggaacgattt tgggctcatc ctacttcatt
    23641 agtcataatc accaagaagc ccaagctcaa acagaaacct caatttcaag agtggaaaat
    23701 tcactaggag caaagacagc ttctacttca acaacagaca acaactatga agcaacacca
    23761 acgacagaat ttaaagtcgg agaagatgtt gtcatcagta ttgatgggaa aactactaag
    23821 gctgaagtac tttccattga cggtgataag gcagttgtga aagctccaga tgggaatcaa
    23881 tacaaaatac aagctcactt aactgaaaag aattaaggag ggctaaaatg cgagaagaaa
    23941 atattgttta tccagtaatt gaaaatcaat caaatacaaa atcttctcaa cttcttcaag
    24001 aagtgcaagt cgtttatccg gttaatgaac taccacaaac gaatgaaaca gtgagcaagt
    24061 caatatcatt aattggcttg cttattgttg caacggcgct aactgttggt gtttatcgta
    24121 agctgaaagt gaaaggaagt atttctgatg gcagataact caaactatgg atttaaacat
    24181 aatttgcatc aaaaaccttc tgaaacaata gtcagagttg attggcttga aaaacatgtc
    24241 aacagcacct attctgttac agcaaaaaga ctaattgttt cagctattgt tgttgcagtt
    24301 gttgttcttg gttcaatcgt ttatttggtt tccgatacga ttaaattgag atttattttt
    24361 attccaacgc tattgatgat aattggtttt cttatactga ttagtcagta tatgtttatt
    24421 gatccatcta gcggatcact gatctcaggc tctattagac tgtttaaatc agtagttaac
    24481 acagcaagag taacgatggg gaaaaagaaa ccatttcgag gaacggagat tttgaaagtt
    24541 agacaagatg gaacgattat gtatcgagat ggtagtgtag cactaatgtt taatttggat
    24601 ggtgctacct cgttaaccgc cttcccagaa gaaatcatag cgcaagaagg caaatcaatt
    24661 caataccaaa atgcaaggga tcgttccttg gctgaaatca aaattacttc atcacaacct
    24721 cagaatacgg aacgtcaaat acggaacatg aaagtacaaa aaagaaatgc aaagagcaac
    24781 aaagctgtgt ttgacatagt agatcaacaa gagaggtttg tatctgaata tattgatggg
    24841 gtcaaaacta cggtgatcca atatcttatt ttgaagtcac aatctcagat ggtattggat
    24901 gaaggcgtag aacgtttaga aaattacacg agtagagggc tttactatag cgttactcag
    24961 ttgtcagcta aacaaaccaa agaaatatta tcagaaattt atgcactgaa ataatagagg
    25021 tgagaaattt aatggaacta atgagtagat cgaagctact taatttcaga aaaaagaaac
    25081 acaaaaaaga gcaaaatgct gatagttcta aagcggtgaa taatacttat gtcagtgatt
    25141 taacatcatt ggttggttat caatttacac catcttacat taggacaggt aatcggtggg
    25201 gaacgattgt taaattagtg aatacgtatg gaatgaacag aaattatgaa tttggatgga
    25261 tggttaaagc tattcctgaa gtgaaggttg aaggagtaaa agcttactta atcgaagcgg
    25321 ataagccagt ttcgcaaaaa gatcagcaag aaatcatgca cgaaaatgtc ggtggatcaa
    25381 cgaacagtta tgaaaataac ttgaacgata aaaattcaga tgcagaaatc aataaaactg
    25441 ttatgcgaat cattgatttg aatacagcat ctctgcttga cagtacggca aacaaaatta
    25501 ttgattggaa gttagaaatc ctcttagtat ccgattctcc taacaagatc agcgaacagt
    25561 tgatcaaact gcaacgcctg tatgatgaaa aaatgtatgg attaaaactt atgtcttttg
    25621 gtggcgatca acgtgatctg tttgataggc ttttaagtga gccagtcggt agtgtttacg
    25681 aatacacatc tatgagttct gattttgcag gaaatgatca cgctatccgt aaaggcttag
    25741 atgatgcaga cggatgggct atcggaacga tgtctacaag ctacgcttca ggtgtagcgt
    25801 ttatggcaat ggatcgttct tttaagggta aagtcttagt agcatcccac aaagatgcaa
    25861 caatcaaagg ctatagcgaa gaaatctcag catcatcact ttgggggcaa ttgattgcta
    25921 ataacgcaat ggcaaatcat aaacggacgt ttcacatcgt attaaataaa tttgactact
    25981 ttggtgatcc gacaggggaa acatttgttg ctaagccatc tttaaatagt gtgatagaga
    26041 agattaatct agctgaagaa ggacttaatt ctttagagct ttttggtgat cgaaaggata
    26101 ttggagattt atatagacgt aatcttgata aactaactca gattttctat ctgttagcgg
    26161 acagagaatt aaatcctgca acaagagtat tgctgaagaa agagttgggg aacttttata
    26221 ttagtcagaa gctttgggat attaactatg aagagcggtc gaaactgtta gtaaacagca
    26281 gtgatacaat gccggttctt ggggatttca tcagctattt aacaaatgct gagacaaaag
    26341 cgaaaaaatc cggtacagaa aaagaaattg atcggattat tgtattaaaa gatacattgc
    26401 gaacagcatt ggatagcttc tctaagatgt tcaatcagag aactacattg aaagaaattg
    26461 gcaagagcga caaattgcag ttttactaca atttgagcga tttaagaaca gaaatcaatg
    26521 tcatggaagc ccaattttta aatacctttg acttcatcac gtcacaagct aaaaaaggcg
    26581 atatcgttat gatccatggg tttgatagat tatcagttga aaccgttgga ttaattaaaa
    26641 agcgactaac aatgcttgaa gaagatggag tccggcttgc gtttctcttt gatactatcg
    26701 gatcaggaga atctaaagac ggtatcgaaa cagctgatat tttcaataca gatggaaaaa
    26761 tttatcaaag ttttgaaagt gattttaact acaccatcat cgggaaaatg accagtgaag
    26821 aattgatgaa gtatgaaaag aaggttgctc ctgcaagaaa aggagaagat gatcaaaaac
    26881 tatcagtgga tttgagacag tatttgacat caggtcgaag caataaattt caaatcagaa
    26941 gaccagcgga tttgactaat aattttgtta ttggagagtt tattatttaa gtcttatcct
    27001 tgtttttgaa tgtttaagga taagaaacaa tatttatata catgactgag agaaagaatt
    27061 atagtggcgg taaaataatg aaatcaaata taaaaataat agcaggtgat aaaatgaagg
    27121 aaaaagaaaa tttagtttgg tattttaaca gaaatccgca tatgctgatt acaggaagaa
    27181 cagctacagg aaaaacttat ctgttgaaaa gaatggctga ctatttatcg caagttggta
    27241 ttgtttctgc ttcttttgac aaaactgaat ttgaagaaat tttatcttcg tctcgaaagg
    27301 aaatggaaga tcggtttgag tatattaaag aaaatcgttt taaaatgttt gacgtaagtt
    27361 actctgattt agacatgaag ccttattttg ttctgataga tgagctagat tcaaggttgt
    27421 tactcttaga aaaagaaaat ccattggctt ataattacct attactaata ataaaggata
    27481 ttgtgttaaa aggacgagta gtaggtgttt tttttattgc cgttcgtcaa ggaaagcaac
    27541 cattgttaga atctatagaa gataatatgt taacacgtat ttttttaggg gattccaaag
    27601 gaaatggaac gatttgtttt gatggaaata caaagaaatt ccaaacagag ctagtaacag
    27661 aagatttctt ttctgtagac tgttagtata gcggaggtta aataatgaaa ccaaacataa
    27721 aaattttaga tcgaatattt ctaggtaaag acacagaagt aatcctaatt caacatgaa
//
LOCUS       DAZUKC010000034        27547 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000034 DAZUKC010000000
VERSION     DAZUKC010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.035"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            119..2560
                     /locus_tag="ACG80T_001391"
     CDS             119..2560
                     /locus_tag="ACG80T_001391"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFS4841051.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKPYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     KVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2890..3594)
                     /locus_tag="ACG80T_001392"
     CDS             complement(2890..3594)
                     /locus_tag="ACG80T_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841052.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3781..4026)
                     /locus_tag="ACG80T_001393"
     CDS             complement(3781..4026)
                     /locus_tag="ACG80T_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841053.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4043..4894)
                     /locus_tag="ACG80T_001394"
     CDS             complement(4043..4894)
                     /locus_tag="ACG80T_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS4841054.1"
                     /translation="MNSLTSTYRLLNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQPTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5060..6064
                     /locus_tag="ACG80T_001395"
     CDS             5060..6064
                     /locus_tag="ACG80T_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFS4841055.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(6305..6661)
                     /locus_tag="ACG80T_001396"
     CDS             complement(6305..6661)
                     /locus_tag="ACG80T_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS4841056.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            7029..8216
                     /gene="tuf"
                     /locus_tag="ACG80T_001397"
     CDS             7029..8216
                     /gene="tuf"
                     /locus_tag="ACG80T_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFS4841057.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(8404..10995)
                     /gene="mprF"
                     /locus_tag="ACG80T_001398"
     CDS             complement(8404..10995)
                     /gene="mprF"
                     /locus_tag="ACG80T_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFS4841058.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLVSVLPMIGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDP
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11021..11782)
                     /locus_tag="ACG80T_001399"
     CDS             complement(11021..11782)
                     /locus_tag="ACG80T_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4841059.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            11949..12581
                     /locus_tag="ACG80T_001400"
     CDS             11949..12581
                     /locus_tag="ACG80T_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFS4841060.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMRSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            12640..13500
                     /locus_tag="ACG80T_001401"
     CDS             12640..13500
                     /locus_tag="ACG80T_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFS4841061.1"
                     /translation="MEKRQKMENQKVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            13685..16000
                     /locus_tag="ACG80T_001402"
     CDS             13685..16000
                     /locus_tag="ACG80T_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS4841062.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            16182..18023
                     /gene="dnaG"
                     /locus_tag="ACG80T_001403"
     CDS             16182..18023
                     /gene="dnaG"
                     /locus_tag="ACG80T_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFS4841063.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            18047..19156
                     /gene="rpoD"
                     /locus_tag="ACG80T_001404"
     CDS             18047..19156
                     /gene="rpoD"
                     /locus_tag="ACG80T_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFS4841064.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            19373..21514
                     /locus_tag="ACG80T_001405"
     CDS             19373..21514
                     /locus_tag="ACG80T_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFS4841065.1"
                     /translation="MMKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDA
                     AMQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIE
                     KNEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHRED
                     TKEEHAKELLKMISEKIAQENKVEPEHEAEKDTEKDISGVNAAGKESLKTNDLKTNDL
                     KTDIPEKTETLSEKQSEDEKSNGLPEADKKEMLEKADKKATESDGINNEADAETQSAS
                     EDSTALEAKIEKEENQPELIEAETEPITIVPNTEKTPQESNPEKKDLDKTQKKKSKKV
                     PYLALAAVLLLGGGGWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFI
                     RADMIHQDLSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDH
                     LADDIKLKADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVV
                     DSATRDQYKTAKEAVEKVKNTDLSAQLNEQLQQVDKMLTDKEKKEEEEKKKAEEAKKA
                     EKAKKAAEEQAKQAAQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARAS
                     DIADASNPAWTWAPGVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKS
                     ALMKGIGESALPFYIVTINCKTGWFGGNGSN"
     gene            21710..22573
                     /locus_tag="ACG80T_001406"
     CDS             21710..22573
                     /locus_tag="ACG80T_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFS4841066.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            22678..23151
                     /locus_tag="ACG80T_001407"
     CDS             22678..23151
                     /locus_tag="ACG80T_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFS4841067.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            23247..24134
                     /gene="xerD"
                     /locus_tag="ACG80T_001408"
     CDS             23247..24134
                     /gene="xerD"
                     /locus_tag="ACG80T_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFS4841068.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            24153..24524
                     /locus_tag="ACG80T_001409"
     CDS             24153..24524
                     /locus_tag="ACG80T_001409"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4841069.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGVYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            24521..25291
                     /locus_tag="ACG80T_001410"
     CDS             24521..25291
                     /locus_tag="ACG80T_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFS4841070.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            25275..25844
                     /gene="scpB"
                     /locus_tag="ACG80T_001411"
     CDS             25275..25844
                     /gene="scpB"
                     /locus_tag="ACG80T_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFS4841071.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISFEEDVSPDLFFDGMNKGE"
     gene            25850..26566
                     /locus_tag="ACG80T_001412"
     CDS             25850..26566
                     /locus_tag="ACG80T_001412"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFS4841072.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     regulatory      26648..26772
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            26895..27506
                     /locus_tag="ACG80T_001413"
     CDS             26895..27506
                     /locus_tag="ACG80T_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS4841073.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIP
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
BASE COUNT         9267 a         4822 c         5796 g         7662 t
ORIGIN      
        1 atataaaaaa gagaatattt aaggattgtt aaaatttaat cttagaaaaa aacaactgga
       61 acaagagcta tttgttttta cagcaaagaa tggtatattt ttgtaggagg tggcttactt
      121 gaaagaagtc gttatgatag atgctgcaag aacgccgata ggaaagtatc gcggaagttt
      181 aagtccattt actgcagttg aattaggcac acttgtgact aaaggattat tagataaaac
      241 aaaactgaaa aaagataaaa tcgatcaagt aatattcgga aatgtcctgc aagctggtaa
      301 tggtcaaaat gttgccagac agattgcttt gaacagcggt ctccctgtag atgtcccagc
      361 aatgacgatc aatgaagttt gcggatctgg tatgaaagca gtgatcttag cacgccagct
      421 gatccaatta ggtgaagcag aacttgtgat agccggcgga acagagagta tgtctcaagc
      481 acctatgctg aaaccgtatc agtcagaaac aaatgaatat ggtgaaccaa tttccagtat
      541 ggtcaacgac ggattgactg acgcattttc aaatgcacat atgggattaa ccgcagagaa
      601 ggttgcaaca caattttctg tgagcagaga agaacaggat cgctatgcct tgtcgtccca
      661 gttgaaagca gcacatgctg tcgaagccgg tgtattttct gaggagatca tcccagtcaa
      721 gatttctgat gaagacgtgt tatctgagga tgaagcagtt cgtggaaata gtacattgga
      781 aaaactgggc acgttacgta cagtattctc agaagaagga actgtaacag caggaaatgc
      841 ttcgccattg aatgacggtg cctctgtggt gatccttgca tccaaagaat acgcagaaaa
      901 taataatctg ccttatttag caaccatcaa agaagtagcg gaggtcggta ttgacccaag
      961 tatcatggga atcgctccaa tcaaagccat tcaaaaattg acagatagat caggaatgaa
     1021 tttatcaaca atcgatcttt ttgagatcaa tgaagcattt gcagcttctt ctatcgtagt
     1081 tagccaagaa ctgcagttag atgaagaaaa ggtcaatata tatggaggag caatcgcatt
     1141 aggccatcca attggtgcaa gcggtgcacg tatcttgact acattagctt acggattgct
     1201 tcgtgaacag aagagatacg gtattgcttc attatgtatt ggtggaggtt taggcttggc
     1261 agtcttgtta gaagcgaata tggagcaaac acataaggat gtgcaaaaaa aaaagtttta
     1321 tcagcttacg ccatctgaac ggcgcagtca attaatagaa aaaaatgtat tgacacaaga
     1381 aactgcgttg atttttcagg aacaaacact ttctgaagag ctctccgatc acatgatcga
     1441 aaaccaagtg agtgaagtgg aaatcccaat gggcatcgcg cagaattttc aaatcaatgg
     1501 taagaaaaaa tggattccga tggcaacaga ggaaccatct gtcattgcag ctgcaagtaa
     1561 tggtgcaaaa atctgtggaa atatctgcgc agaaacacct cagcggctga tgcgagggca
     1621 aatcgttcta tcaggaaaat cggagtatca agcagtgatc aatgcagtta atcatcgaaa
     1681 agaagagctg attctttgtg caaatgaaag ttatccttca atcgtgaaac gcgggggcgg
     1741 tgttcaagat atctctactc gggaattcat ggggagtttc catgcatact tatctattga
     1801 ttttttagtg gatgtaaaag atgcaatggg cgctaacatg atcaactcga ttcttgagag
     1861 tgtggcaaac aagttacggg agtggttccc tgaagaagag attctattca gtattttaag
     1921 taattttgct acagaatcat tggcttctgc ctgttgtgaa atcccattcg aacgcttagg
     1981 gagaaacaaa gaaatcggtg aacaaattgc caaaaaaatc caacaggcag gggaatatgc
     2041 gaagcttgac ccttaccgtg cagcaacgca caacaaaggg atcatgaatg gtatagaagc
     2101 tgtagtggca gctacaggaa atgatacacg tgcggttagc gcttcgattc acgcctacgc
     2161 ggcaagaaat ggattatacc aaggattaac agactggcag atcaaagggg ataaactagt
     2221 tggaaaacta acggtgcctt tggctgttgc cacagtaggc ggagcttcca atatcctgcc
     2281 aaaagcaaaa gcatctttag ctatgttaga tattgattca gccaaggaac tagcacaagt
     2341 aattgctgca gtagggttag ctcaaaacct ggctgctttg cgtgcattag ttacagaagg
     2401 aatccaaaaa ggccacatgg gacttcaagc tcgttcatta gcgatctcaa ttggggctat
     2461 tggtgaggaa atcgagcaag ttgcgaaaaa attacgtgaa gcagaaaaga tgaaccagca
     2521 gacagctata caaatattgg aaaaaatccg ggaaaaataa aaaagcaata tctttagaag
     2581 aataaagcaa gatttacagt tattttattt actgcaaatc ttgctttttt ctctatcaga
     2641 aaatatgttt attattagaa ttttttatag ttaaggttag gcatcttaag aaatcaggca
     2701 actccaagat atcttcaggt agaagagaag tcgcctaaca ttgtaggatc ataggtgatt
     2761 tgttggacag gtcaagctga tatttgagcc tgatgttaga aggaatttct ttttacttgc
     2821 tgtagaaatt attatatgac taaaaaggct gggattttaa atcccagcct tttatgacaa
     2881 atagaatttt tatcgatcag taatcgctgt cgctgcaaat tgttcgatca aaggaatcaa
     2941 agtcaatttg actttgtatt tccgttcgag tgctttttcg ataacgaact cattttcagt
     3001 agtcatagaa atagttgcta atttacgagg ttcatcagca tttttttcat taataactac
     3061 ttcatgttca ttttgttctg tgtaaaattt aggatactca gcaacggctt ttgaaaaatc
     3121 agcaaaactt aaagcggcat tcgtatcagt tgagcgtgta ttttcagttg tgtccgcttt
     3181 tggtttttcc gttttttctt ctggcacact gactgtttct tctgtaactg gtgtcgcaac
     3241 agcttgcgca gttggttcat cgtttttcaa tcgttgatca ttccagtaag acacgatttc
     3301 aataagaaga ttgtcacgga atcgttcgaa aatcccgttt tctcctgaac gaattctctt
     3361 taatacagta ttgaagttgt catcgattgg cacgttgatc cgtgtatctt catttgtttt
     3421 cccagctaat ccgatcgtta agatttgacg gtttttggga tcaagcatga cttgtaacgg
     3481 caaaccatag cgtcgcaagc tctcttcttc tagagaacgg aataattgtc ctcttacacc
     3541 atccaatgaa ggacggaatt gatttaataa tttttgtttc tgttttttct tcatatgttc
     3601 tctccttcgc tcataccctt tcgattataa gaaaaaaaca gccaaatgaa aaggagtaat
     3661 gcaaaaaaac ttgcgtatac gcaaattaca acagaaaaat ttgcgtattt tcccgttttt
     3721 tgaaagaaaa ataaaaaatg gaacggatta tgtatatata cataatccgt tccattcttt
     3781 ctattttttc ggtatcaagg caaaaacagg tagttcaggt gaataaatga cttcatgttc
     3841 gtgccagggt ttgaccatta gcgaatgatc gaaccgtttt ttcagttctt cgatgatttg
     3901 atcatgtgtc cagtttcttg caggaaaatg ttggattttc tctgcatcga tcaaaaagac
     3961 aaaatcggaa tgaaaagtct gggtaacttc ttccgagaga gaattcaagc gttcgattgg
     4021 atccataaaa tcacctttcg acttaaaatt gggtagtatc tgggtctttt gctttgccga
     4081 ctacaccatc taactgatcg atttgtttca tttcttcttc tgttaattca aaatcaaata
     4141 attcagtatt ttctttgatt ctgcttggtg tcactgactt aggaagagga aggaattcgt
     4201 gttgcagcga ccaacgcaaa gcaacttgtg caatcgtctt gtcatgtgct tcagcgattt
     4261 gtttcatttc aggcacatcg aagattttac cagtgcctaa tggactatat gcttcgagga
     4321 tcatatcatg tttttttgca tactcaacta cagttggctg taattcgcct ggtgcaagga
     4381 aaacttgatt gaccatcggc ataatttttg ctgtttttgc tagtgtatct agatggtggg
     4441 gtaagaaatt gctcacaccg attgctttga ttttacctgc ttcatacagt tcttcaaatg
     4501 ctcgccatgt atctgcattt gcttgttccc aattatcacg aaaagcaaca gggtttggcc
     4561 agtggatcaa aaacagatct aaataatctg tttggagctt cttcaatgat tcttcaaaag
     4621 aagacatcac aagttcatag ctgtggtttg cattccataa ttttgttgtc aagaatatct
     4681 cttctctagg aattccactt tccttaatcg cttggccgac actttcttca ttcttgtatc
     4741 cttgggcagt atcgatatgg cgataacctg cagccagcgc ttctttaaca gaggaaacag
     4801 cgacatcccc atcaggtgtt tgccatgtgc cgaatcctac taccgggatt tcgtaaccat
     4861 ttaatagacg ataagtgctt gttaatgaat tcatactttc agctcctttt ttattcgttc
     4921 aactataagt gtagcgcatt tttcgaaaaa aagaatccat tttgtttcta ctcttgaaat
     4981 ttttgtttaa aagaaaatga ttttgattct ggtatttttg attttttttc cgtaaactaa
     5041 gacagaaggg aatgagaaaa tggatatcta tttaaaaaaa gcaattctgc atattattga
     5101 cagagaaact ggttctccag tcttttcaca aaaagagctg gatctgacaa aagaatatat
     5161 tcgtgatttt cttcaaaaga agatccaaaa aatctcttct gctcaaacaa aaactggaca
     5221 actagcagaa gaaagcatga tcgttgaatc acttaaaatc gctcaagaaa attttgtgga
     5281 agcaagtgaa gcaatcgttc agcggtggtt cgatatctac caagaaagtg aagaagcacc
     5341 tagtgcagat gtatttgttg ttttgtatga attagataca gtgatgtact tatctttatt
     5401 gaaagtcaat tatcgtgagg cttacactca ctttgttgaa gcagacgaag cagggatcga
     5461 caaccgcttg attttgaatc gagcaattct aggggcgaaa acacaaaaag cagatgaagc
     5521 attcgctgtg aatttgactg attggaccta cgaattaatt gaaaaaaaat atgagttttc
     5581 tggcaaaaaa gagctttatt tttctggaag agtgatcgaa agtgttcctg caccttcttt
     5641 agaagaaaat gtaaaagtga tccaaaaagt agccaagcat cttggaaaaa aatttgagac
     5701 agaagaattt gatatcatgg ctgatttgaa agaagctgtc tatgatacga tagaagaaaa
     5761 aggtcggttg gatcatgaga tgattgcgga aaaagtcttc aaggaaaaca tcacggcaaa
     5821 aatggctttt aaagaagaag tacaagagca aggatttgtc ccagaagcac ctcctgtaaa
     5881 agaagtacaa gaaatttctg agaaaaaatt tggaaaacaa aaattcaagc tatcaaacgg
     5941 gatcgaactg atcgtaccag tagatgtata tcgcaaccca gatttgattg aatttgtgaa
     6001 taatccggat ggtacgatat ccgtgatgat caaaaatgtg gatgaagtat tgaatcgttt
     6061 ataaaaatga ctagaacatt aggtgttcaa gtaccagtga tcagttaaat gaattgtgtc
     6121 aagagaagga tcggaaaaga cgaatataac aaaaaaagag cgtgagacaa aagtaaagaa
     6181 cacttttgtc tcacgctcta aacacgtata aacggcggaa gcagaagcaa ctccttctta
     6241 tttcttggag ttaaacgctt ctgtcccagc ctctttttat ctaataaaat tttaagacca
     6301 tttactaatt atcgattttt tctgccgcat ttttaggcac ttcagaagcc tttatttctt
     6361 cccagcttaa tacaccttta cgaggattga ctttgagttt aagatacgca ttcatcttta
     6421 atggatgttc tctttcttcg cgaagcgtca cctgtttttt ttcaccatct ttattataag
     6481 ccgcttgctt gtaggtataa gtcgtgaatt tctctccgtt atctgcttgg gtagctgaca
     6541 tttcgccatt cgtcgtgatt tttgtataat aagcttcgcc gccgtaaaaa taatgataag
     6601 ctgcaaaact tccgccgaga aatatgccta aaacgacaag tacgccaatt aattttttca
     6661 tgtacttcac ctctttcctt attattaacg aaatagaaga tttcgtctat tgaaagttgt
     6721 tggcaacagc tgaattttaa acttttcctc attttgttct tattgcttca aaagaaagtt
     6781 aaggactatc ttgaagtttt tctcaaaaaa cacatttttt tatttataaa aggtactttt
     6841 ttcgcgtata agacaagcga gtgctcttag ttgactttga ccaatattga cgtataataa
     6901 gcttagttcg aatcttacaa aaagtaagtc ggacgtattc tttttgattt agtcaaaaaa
     6961 agactataat aaaaaggtga gagctgaaat ttttcagtga tctatattat ttaggaggtt
     7021 tgtaaattat ggcaaaagaa cattatgaca gaagtaaacc acacgttaac attggtacga
     7081 ttggacacgt tgaccatggg aaaactactt taactgctgc gatcactaca gtattaggca
     7141 aaaaaggcct agcaaatcct caagactatg ctagtattga tgcagctcca gaagaacgtg
     7201 aacgtgggat tacgatcaat accgcacacg tagaatatga aacagaaaaa cgccactacg
     7261 cgcatattga tgcaccagga cacgcggact atgtgaaaaa catgattaca ggggcagcac
     7321 aaatggatgg tgcaatctta gttgtttctg caactgacgg tccgatgcct caaacacgtg
     7381 aacacatttt attgtcacgc caagttggtg taaaatacct gattgttttc ttgaacaaag
     7441 ttgatttagt cgatgatgaa gaattgatcg atttggtaga aatggaagtt cgcgagttat
     7501 tgagcgaata tggtttccca ggcgatgaca ctcctgtgat caaaggttcc gcattaaaag
     7561 cattgcaagg cgatccagat gctgaagctg ctattatgga attgatggat acagtagatg
     7621 aatatatccc aacaccagaa cgtgatacag ataaaccatt actattgcca gtggaagacg
     7681 tcttctcaat tacaggtcga ggaactgttg cctcaggtcg tattgatcgt ggtgctgttc
     7741 gtgtcggtga tgaggtagag atcgtaggga tcaaacctga aacacaaaaa gcagttgtaa
     7801 caggtgtaga aatgttccgt aaaacgttag attacgggga agctggggat aacgtaggcg
     7861 tgttgttacg ggggatccaa cgtgacgata tcgaacgtgg acaagtgctt gctaaaccag
     7921 gttccattac tccacataca aaattcaaag cagaagtgta cgtgttgaca aaagaagaag
     7981 gtggacgtca tactccattc ttcaacaact atcgtccaca attctacttc cgcacaactg
     8041 atgttacagg aacaatcaca ttgccagaag atacagaaat ggtcatgcct ggtgacaacg
     8101 tgacgattga tgttgacttg atccatccaa tcgctgttga aaacggaacg acattctcaa
     8161 ttcgtgaagg cggacggaca gtgggttcag gtatcgttac agagatcgaa gcataagaaa
     8221 cgcgactcga atattttggt aatgaaaaat gtttaaaata aaagagcggt ccaaacagga
     8281 tttctgtttg gaccgctctt tgttcttcaa ttgtataaga agagattgta gaagcatgaa
     8341 ctttcgtttt acttcagtct cttttattca tcatgagagt gttcatctat cggtatgcag
     8401 tttttaatac tttcttcgta tccatttttt caaaccactt attctacctt gtgtctgctc
     8461 aactggcgca ttatcaataa tcagcaaagc aatcataaca tatgcaatcc aactatctct
     8521 agaatataga gtataacgtg agacccagtt tgtagcgtac ttgcttttat attctcttaa
     8581 tccatggaaa gaataaaaat gagaaccaaa ctcgtaaacc aagtatgcaa tcctttcctg
     8641 taaaaaactc tttctggaag ttccgacatt tgaaagaggg gccatcccta aattgaacca
     8701 ttggatatgt tcttctttca tataggcaaa caaatgcaag aacaagaaat ccatactgcc
     8761 agaaggtgct tttttaggat catatcgcat cagatcaatc gttcctactt tattgttcgt
     8821 gtaagtaggc atgatcgttg caaaagcgac aatttcttct tgggtatttt tgacaactgc
     8881 aataggcgct ctttgtagat aatcttcaga gaaaaagcct aaagaaaacc ctttttcctt
     8941 tcgtgaaccc aaccaattat cagagatatt tttgaaagta ctcatttgct cagtagaaaa
     9001 tggtggttgt aagacatcaa atgtaaatcc ttctcgttcg atccgattca agacagcacg
     9061 agtgcccttc atttttttcc ctgaagttgt aaaactattc aggtctacat aagcttcttc
     9121 ccccatctta atgaaatcgt agccgaattc atgcaaaatc ataacaatct cttcgcttgt
     9181 ttcgtagaaa actggtaaat agcatagtcg atctgtttct tcgataaaag cttcgattgc
     9241 ttctggaaaa tcttctttct taccagaagg atcacccatt acaatacatt tattattgaa
     9301 gcaagcgaat tggagaagaa cagttggttc accgtctttt tcataggcaa acatccgctt
     9361 atcttttaag aagatcaatt gactatcgga gtttcctcca taagttgtaa ggatcttcag
     9421 ggccttttct tcattaaagg cttcgccaat ctgtttcttt tctccttgaa ggaaatgaac
     9481 aaatagaacg atcatgaaac taacagcgat gatggccaac aatcccgaaa accaaatctt
     9541 ttcagaagga aataagaaaa aggaaataaa atggtgacgc cgatgaggga aatccggcag
     9601 attgtatacc ccaataacga tgtaaagcaa ggacagtgca ccaataatga ttccatcgat
     9661 cgtacgccac tcccatgaat agatcaattg ttcccgaaat aattcgtttt tagaagcaag
     9721 aatgatcaga agcagaatag acaagaaaac caccggtgtg aaactaaaat cacttaataa
     9781 aacataaagt aaagctagaa caatcaagaa gattgtaggc aaatatgctc gtttcacacg
     9841 tgctgcgatc cctctgccca taatgattaa aagaaatccc aatagtatac taggaaattg
     9901 gataataaaa tgaaatttca atgggttcaa actatgaagc cagcgaaact ctgtaaaagc
     9961 ttgcgggata gtggcagaaa gtactagcat gattccagaa aagtataata agacaaccac
    10021 gattttatgc gcaatttctg ttgctaattg tttagggata ccagaataac gctgatcaaa
    10081 agtggaaccg atatttttga agaaaaagac gattccaatc aaaaacggaa tgatgtagta
    10141 gaacaggcga tagagcaaga tccacacaac gactatttct cttggcacac caatagcgga
    10201 caacccgata atcatcatta catcgaaact cccaatttct cctggaatca tagacacgat
    10261 tcctatcact gaagcagcaa tgaataacgg aattgtttgc cacagatcaa tccggatttc
    10321 cattaaataa ccaacagaaa ggaaactaaa caggacaccg gtccattcca acaatgaaac
    10381 tagcaatagc tgtccttgag ttttaagtga ggtaccacca aggaatcctt tctttttgaa
    10441 agtagtaaat aaaaagacga caggaaaata tagaccgccg ccgatcaacc atatccaata
    10501 ttgcttcaaa aaaggagcaa ctggcgtgaa gactacaagt aaaaaagaaa gcagactgta
    10561 aatagaaagt ccagccatca aaaacaagaa aactttcgat aatgcttgga tcacgtttct
    10621 gctctctttc ttttgtccat atagttcaga gcgaagcccc acactaacaa acccgccgaa
    10681 accggcaata ttgttgattg tattgatcag ccagcttgtc tcaaacaagt aacgaggatt
    10741 ctgcttttgt tcaagcatcc gattcaaaat aatatcataa ccaatcatag gtaacacaga
    10801 gacaagacca atcaacagca taagtccgct tttccataca ggtatagtgg caaacgtttc
    10861 tcctaattgc tggatagata gagttttacc gatggacatc aattcaccga caataatgac
    10921 aatgacagaa atcaaaaaga ttgttttgaa cagtcctagg tggtttttca gccattgtat
    10981 ggtatttttt aacaaataaa tcaccttcta ttcctaatag ttatgtttca gaaagaaatt
    11041 gaagaacttc tcgattgaag cgttcaggat cttttctagc taattcatgt ccttgttctt
    11101 tcacaagcac aaaggaagct tttggtatag ttttagctat atacaaagaa tgttttagct
    11161 tgattacatc tttttttccc acgatgatca aagtggggga gttgattttt tttaaatcat
    11221 tttctgtaag cccgatgtca tgcagcaaaa gtttgataac taaaagattt ttgcgaagac
    11281 ttgggcgaaa taaagagagt aaccataccc atgcataatg aatattactg atgactctgg
    11341 cagaaaaacg aacgccttta acaagcgtgt ttccagaatt taaaatcaat cgatgaactt
    11401 ttttagggaa gctgctggca aataccagtg ctagatttgc tccatcacta aatccaagaa
    11461 aatctgcctg atcgattttt tccagcaaca tgatcgtatt cagatcctcg gccattaact
    11521 gaaaattcaa catagatgct tcgtttgtcg aacgaccatg tcctcggcta tctactagat
    11581 acacggtata gtagcgttcg agtacaggga cctgttctga aaaaaagcgg ccgctgccgt
    11641 cattgccatg cagtaaaaag agagggaagc cttgaccgct cttttcatag taaatttcgc
    11701 tcccatctgg catttttgca tatttctttt cttttttcac ataatcacct actgtgtaaa
    11761 gtataacgat tatatcagtc attgggcaat tgtttttact gaaaaacaac gatcattttc
    11821 gtaagatgac aaaaaataca ggaaaattca ttttttctta aggcgtacac agaaatcaag
    11881 gaagaaatag ccaagaagat aaagaaatag taagataaaa aaagatgtca aaataactag
    11941 gtgatgaaat gaagcgaaaa cgattatcta ttatccgtat tcttttttac atgatcattc
    12001 tgacggttat tggtgcggtg ttgtttttag gaggaagaac ttatcgttcg gtaaaacgag
    12061 tggaaagcta tcaaatagaa ataacaacag ctgcaaaaaa ttatcatatg gaaaactacg
    12121 agaatttgat caaggcgata atattgaccg aatcaaaagg aaaagggaca gatctcatgc
    12181 ggagctcgga aagtctgcat ggagaagctg agatcattga taatccagct gacagtattg
    12241 atcaaggtgt caaatatttg gcggaaatga tagtagaagc aaagaaacaa ggatgcgatt
    12301 tatggacagc tgtccaagca tataattttg ggaaagatta tattgcctat gtaaaaaatc
    12361 atggcggaga aaacacagta gaattagcag aagcgtattc taaagatgtg ctgagcccac
    12421 ttttaggaaa tgaggaaggg aaacaatacc gatattggcg gatccagtcc ttgctttaca
    12481 atggcggcta tctctatcat aatggaggta acatgtttta tcctgatatt gtcaaaatga
    12541 atgaaaaaat aatagagacc tatgaaaaag tttttaagta aaatagcagg aaatgttcaa
    12601 tcatgaataa aattaaatga aagaaggagt gcgcaaatag tggaaaaaag gcaaaaaatg
    12661 gaaaatcaaa aagtggggta cacaaccaaa gactggatgt tacggtttgt gaagggaatg
    12721 tttatcggtt caggatttat cttgccagga gtcagcggtg gtgcattagc agctgtcttt
    12781 ggcatgtatg agcggctgat ttctttttta gcccacctta caaaaaattt caaagaaaat
    12841 gttttgtttt ttctgcctgt tggtctaggc ggagtgacag gcatctttct actttctttt
    12901 ttagtcagtt ttttattagg tgcgtatgaa acaacgatat tatggttttt tgtcggatgt
    12961 atcgctggaa ctgttcctgc gttatggaaa gaagcaggga aaaaaggaag agatacttct
    13021 gatgtgatca tcatggttgt tacttttgtt ctggcttatt tgtttttatt atacggcgct
    13081 caattatttg acgggcaagt cgatcagaat tttggaacat ggctgattgc cggcggattg
    13141 attggtttgg gtatgatcgt cccaggattg agcccttcta attttttggt gtatatggga
    13201 ttgtataaag cgatggctga cggatttaaa gaagtacgac ttgaagtgat tgtgccgatc
    13261 ggaattggtg ggattctttg tgttcttgga ttgtcaaaaa tcatggatta cattttctca
    13321 aaggcatata gcaagctatt ccatttcatt ttaggtgtag tttttgcttc aacgattatg
    13381 atcattccta ccgattacgc gggattggga gttgctgggg tactagcttg tcctgttctt
    13441 tttatagccg gtacagcact aggttggtgg atgagccgtt tggaggaaca atataagtaa
    13501 agatagattg ataataaaaa cagcacacgg gtgattcaaa caaaaaggtt ggcatctttt
    13561 ttgtaaaatc attcgtgtgt ttttctataa attttataaa aacaagttgt ttttaatctt
    13621 ggaacacgct agacttaaac acgtgattat ttttgaaagt tggcggaaaa tggagaaaaa
    13681 gaaaatgaat ggacttacac agcaagaggt agttcagaaa atggcttctg gccagcaaaa
    13741 tgactaccag gaagatgcat caaaaagtac aaaacaaata ttcagcgata atatattgac
    13801 gttattcaat tttctgaact ttggtattgg gatttgtctg ttatttgtag gtgcgtattc
    13861 caacatggcg tatttagcaa ttattgtcgt caatatcgtt attggaattt atcaagaaat
    13921 acatgcaaga aatcttgtac ggaaattatc gattgtttcc caatcagaag tactagtgat
    13981 ccgggacggt aaagaacaat cgatttctac taaagagctg gtattaggag atttggtaaa
    14041 aatcggagca ggagaacaga taccagccga tttacaggtt atatctggac gggcagaagc
    14101 aaatgaagct cttttgacag gcgaatctga tttgattgtc aaagaaactg gagctgaatt
    14161 gctttctgga agctttttga caagcggttc tgtcatggct gaagttatcc gagtaggtgc
    14221 agataattat gctgtccgct tgactagcga ggcaaagact cacaaagcta ttcgttctga
    14281 acttgtagat tcgattcgca aagtatccaa attcactagt ttcgtgatta ttcctttggg
    14341 gatcatacta tttttggaag cactgttcat ccgcgatgct tctatcaaaa tttctgttat
    14401 tgcatctgca gcagctttgt taggcatgct tccaaagggt ttggtattgc taataagtat
    14461 tgcattaacg acaggggtaa ccaaactggc aaaaaaacga atccttgttc aagacatgta
    14521 ttcaattgaa acgctggccc atgtcgatac attatgcttg gataaaacag ggaccattac
    14581 tgaaggaaaa atgagtatcc agcgggtaga agcactacat ccgctgtacg agaaagccat
    14641 cccagaaatt atggggacgt atctggcaga aagcagtgat aacaacatca ctatgcgggc
    14701 tatgagagat tattttgagc tttctaatcg gtatgatgtg acaaacaaag tcgcattttc
    14761 ttctgatcgc aaatggggcg cgatggaatt tccagaactg ggagtcgtgt acgtaggagc
    14821 gcctgaacgt ttggtggaag aagatcggct tccggacagt ctaaaaacag cccaagcgaa
    14881 tggctatcgc gtacttatgt tagggatcgc tccaaatcag caattgaacg aagaaaaacc
    14941 agcatttgtt gagccggttg ctctgtttga aatcgatgat ccaatccgaa aaaacgctca
    15001 agaaacacta gcttatcttc atgaggaagg tgtggatctg aaagtgattt ctggagacaa
    15061 tccttttact gtctccaata tcgctagacg tgcaggatta ccgaattatg atgcgtatgt
    15121 cgatctttct caaattactg aagaaatgga agtgcgagaa gctgctcatc gctatacggt
    15181 ttttggacga gtgagtcctc agcaaaagaa attgctggta aatgaattga aagaaagcgg
    15241 cagaacggtt gcaatgacag gcgacggtgt gaatgatgtt ttggcgttaa gagaagcaga
    15301 ttgtagcatt gctatggcag aaggtgatgg agcaacacgt cagattgcga atctggtatt
    15361 gttggactct gattttacta cattgccaga agtcctgttt gaaggacgcc gagtggtcaa
    15421 caatgtgacg aaagtctcag gcatcttttt tatcaaaacg atttattcat ttattttatc
    15481 gattatatgt gccgttacag caattggatt tccgtttatc cctatccaaa tcacgctgat
    15541 tgatttagcg attgaagggt atccttcctt ctttttatct tttgaacaag ataaaagaaa
    15601 aatcacttac cgttatttgc ctacagcact tattaatgct ttaccaaatg ccttgcttgt
    15661 agtagtgaat atcattgcgg tgtatctact tggaaaaaca ttcgcatggg gaaatgctga
    15721 aactactact ttgatgtatt acttgctgat tgggatcagt tgcatggcag ttgtaaaagc
    15781 ttgttatccc ttcaacccat tgcgcatctt cctagcagta acgacgatcg tagggatata
    15841 tgtagctgca atgctgttcc atggaatttt ggaagtaaac ttagggttgg gagagagcgt
    15901 aggatacttc tttgtattca tgctcgtaaa tattgttctt cgagtattgt actggaaggc
    15961 tgatattcaa aatcagttac tgaaaagagt agcaaattga tgcttaatga gatagaaatg
    16021 tgttatactt gaaaaagcaa tgaaatatga tacaatcaaa catgtataat tgtgtccttt
    16081 aaaatcgcac tgatttcagt tagtgcgatt ttttaccggt tattctcctg aaatatcttc
    16141 tgttaaaccc aaaagatatc aatagagcag ggggtggaaa aatggctcaa cggattcctc
    16201 aagaggttat cgaaacagta cgcagtcaaa cgaatatcgt tgaggtgatt ggacaatatg
    16261 tccagttgaa aaagtctggt aaaaattact tggggctgtg tccttttcat gaagaacgta
    16321 caccgtcttt ttctgttgca gaagacaagc agatttttca ttgctttggt tgtggtaagg
    16381 gcggaaacgt gtttactttt cttcaagaac tagaaggtct aagttttcca gaagcagtgg
    16441 taaaagtggc agacttcgaa cagataccgt tagaagatca atggcgtcag cgtactatag
    16501 ctgtccaaaa tcctgaatct gctacgggga aactgattgc tgctcatgaa aaagcagcgg
    16561 aaatctatca tcacatgcta ttgcatacaa aagctggaca gccagcttta gaatatttgc
    16621 aaagtcgtgg gttgtcttta gaattaattg aagaattcaa cataggtttt gcacctaatg
    16681 agcgttcttt tcttcagcaa gtattcaaga atgaatcaat ttcaaatgaa ttgttagaac
    16741 gctctggatt gtttgtcacg catgatgatg gccgtatgtc tgatcgtttt tatcaaagag
    16801 tgatgtttcc acttcgtaat tctcaaggcc agacgatcgg tttttcagga cgctggcttg
    16861 cacctgaaaa agggcaggaa aaagatcagc ccaaatactt gaatagtcca gaaacagaac
    16921 tgttcaataa aagacaagtt ttatttaatc ttgataaagc aagaagcgat atccggaaaa
    16981 acggagaaat tttgttgttc gaagggttta tggatgtgat tgctgcatgg caggctggta
    17041 tcaaaactgg attagcttct atggggacaa gtctaacagc tcaacagatc caacaaatgg
    17101 aaaagctgac gaaagaattg gtcttttgct atgacggaga caacgcaggt tttgaagcaa
    17161 ccaatcgagg gattgaattg cttcggcaaa acagtcgctt agcactttct gtcgtagtcg
    17221 ttccagaaaa gcttgatcct gatgaatatc tccgtaagta tggagaagca tctttccaag
    17281 aattagtggc tcatgggcgc gaaacagtct ttacttttaa atcacgttat ttaaaacaag
    17341 ggaaaaattt agataatgaa aaagacaaga tcgaatacct cgaagaatta ctagttgaac
    17401 ttagccgagt tgtttcacca atcgaacaag atatgtatct atctcaactg tcaacagagt
    17461 ttcagatcgg cagagagacg atacaaaaac aactgcgcga tcttcgaagg acaaatcagc
    17521 aaaatcgtca agaacctcag gaaagtcaac acactcaggt tacgagacaa gaaactgcac
    17581 ggaaaaagag acctttgacg caagtagaaa aggcagagca gattctatta tatcgtgcat
    17641 ttaaagaagt ttctgtccgt aatattttaa aagaacgaga atttcagttt attcatgatg
    17701 catatgcaga agtttatttt ttattcgatg catatgtctc acaacattat gaattcaacc
    17761 ttgcagagtt tttagacttt ttgcaagatg aaaatttaag gcggttggtg gtggaaatcg
    17821 aatatttacc ggtggcagaa gaaagttcgc cacgagaaat ccaagacttg ctagatgtga
    17881 tcagcaaatc tggcttagct gatgaaataa cattaaaaaa acaaatgcag caagaagcca
    17941 gacgaactgg aaacaaacag cttgagcttg aattgacgat tgaaatcatc aatttaacga
    18001 aacagttaaa acaagccaaa tagctactca aggggggcct tcttttatgg caaatggaac
    18061 agataaaaaa acgtatgaag cagcagtagc agctttcatc aaagaaaaca aacccaaacg
    18121 acaagttgta tatgatgatt tatccaatca gcttgctaca ccattcactt tgaatgcaga
    18181 tgaaatggac aaattgatcc aaaagattga agacgcaggt atcagtgtgg tggatgaaaa
    18241 tggtgatcca tcaattcaca gcttgaagag tgctgagaaa aaagctgaac aagcaaaaac
    18301 agaagatcta tctgcgccaa ccggtgtaaa aatcaatgac ccggttcgta tgtatctgaa
    18361 agaaattggc cgtgtctccc ttttaactgc ggaagaagaa gtagcgttag cattgaaaat
    18421 cgaagaaggc gaccaagaag cgaaacagcg tctggcagaa gcaaaccttc gtttagtagt
    18481 aagtatcgct aaacgttatg taggacgagg catgcagttc cttgacttga tccaagaagg
    18541 aaacatggga ctgatgaagg cagttgaaaa atttgactac cgcaaaggat ttaagttttc
    18601 aacctatgct acatggtgga ttcgtcaagc catcactcga gcaatcgctg accaagcacg
    18661 tacgatccgt attccagttc atatggttga aacaattaat aaattgatac gtatccaacg
    18721 tcaattgctg caagaccttg gaagagaacc tacgccagaa gaaatcggtg cggaaatgga
    18781 tcttccaaca gaaaaagtcc gagaaatctt gaaaatcgcg caagaacctg tttcacttga
    18841 aacgccaatc ggtgaagaag atgactctca cttaggtgat ttcatagaag atcaggaagc
    18901 aacaagtcca gcagaacacg cagcttatga gttactaaaa gaacaattgg aagatgtact
    18961 agatacattg actgaccggg aggaaaatgt ccttcgctta cgttttggtt tagatgatgg
    19021 ccggacgaga acattggaag aagtagggaa agtatttggt gttactcggg aacgtattcg
    19081 tcagatcgag gctaaagcat taagaaaatt gcgccatcct tctcgttcaa aacaattaaa
    19141 agatttctta gaataagcat gaactattga ccagaagaga aatcagtaaa actgtttggt
    19201 ttattttaaa agaaaaaaag cttgagacta cgcagtctca agcttttttt tctataggaa
    19261 ggaaattcat tcattatttt taagtaaatc ctaaaaataa gctattggcg tgacgtatag
    19321 aatatgatag attaaatggt agcgtaaacc tagtaaagga gccgatcgct aagtgatgaa
    19381 aaaatgtcca aattgcggat cgaaaaaaat ggagcagaat actatctgtc cagactgcgg
    19441 tcatcaaata actgcagaag atcagcaaat aaaagaatca attgaagaaa caaatacaga
    19501 tgcagctatg caggataaat caactgaaga tgcagtcttt cctgatacag aattaaacga
    19561 tccaatcgaa tggtcggaat tgaaagattt acctctagaa tctgtaatgg aattattcga
    19621 aaaagcagaa ccagaagacg aagaaccacc tgctcacgat caagtaacta atatagagaa
    19681 aaatgaacag aagaaagcaa ctgttcatga gacttcggaa gaatcaggaa agaatcaaga
    19741 agaaacgctt gaatcaaaac aaaaaagaag agtagctgaa ctgaaagaaa tcgtcgataa
    19801 tgaggaagaa aattcgatac tatccgctta tatcaaagca catcgtgaag atacaaaaga
    19861 agagcacgcg aaagagctgc tcaaaatgat cagtgaaaaa attgcacagg aaaacaaagt
    19921 agagccagaa catgaagctg aaaaagacac tgaaaaagac attagtggag taaacgctgc
    19981 aggtaaagaa tcattaaaaa cgaatgatct aaaaacgaat gatctaaaaa cggatatccc
    20041 ggaaaaaact gaaacattgt ctgaaaaaca atcagaagac gagaaatcga atgggttgcc
    20101 ggaagcagat aaaaaagaaa tgctcgaaaa agcagacaag aaggcaacag agtcggatgg
    20161 aataaataat gaagccgatg ctgaaactca atctgcaagt gaagatagca ctgctcttga
    20221 agctaaaata gaaaaagaag agaaccagcc tgagctgatt gaagcagaaa cagaaccaat
    20281 aacaatcgtg ccaaatactg aaaaaacgcc tcaagaatca aacccagaaa aaaaagattt
    20341 agataagaca caaaagaaaa aatcaaaaaa agtgccgtat ctcgctttag cagcagttct
    20401 tctactaggt gggggaggct gggcttatta tgaccatcat caaaaagtag aagccgaagt
    20461 agcagcagaa gcaaaaagac agcaacaaat ggaagaactt catgagaaac ttgctgcctt
    20521 ttatacagat gatcaccatc aatttattcg tgcagatatg attcatcaag atttatcaaa
    20581 actaaaaaat gatttatcca gtcttaagaa tgaaaaagga tattcagagt tagaaacaac
    20641 ttaccaagat atccaatcta agatccgaga tatccaaaaa gtgaatgagt ggttcatagc
    20701 acctgccatc gtcgatgatc atctagcaga tgacatcaag ttgaaagcag ataaagagat
    20761 ccaagcaatc gaaacggata acacagattt tggaaaattg attcaacaag cacaaacaga
    20821 agctaaaaat caatatgctg aattgcaaac agctaaagaa aaaacagcta tagtctttaa
    20881 agacggaaaa gtagtggatt cagcaacacg cgaccaatac aagacggcaa aagaagcagt
    20941 agaaaaagtg aaaaatacag atctatctgc tcagttgaat gaacaattgc aacaagtaga
    21001 taagatgctc actgataaag agaaaaaaga agaagaggaa aagaaaaaag cagaagaagc
    21061 gaaaaaagca gaaaaagcga aaaaagcagc tgaagaacaa gcaaaacaag ccgctcaagc
    21121 agcagctgca gctcaagccg ctcaacaagc tgcggtcgca caagcagcac agcaagaagc
    21181 ggcatcctcg accccttcct cttcaaactc agcgaatcag ccaatcaaat ctgccagagc
    21241 aagtgatatt gccgatgcat ctaatccagc atggacgtgg gcaccaggtg tccaaagcaa
    21301 agtgattgct acatgtatcc aacgtggata tatcgttgaa ggaggataca gattagaaaa
    21361 agccagaatc gaaaatgggg aaggctatta caatctatat gccacttcaa caaaatctgc
    21421 attgatgaaa ggcattggag aaagtgcttt gccattttac atcgtaacca tcaactgtaa
    21481 aactggatgg ttcggaggaa atgggagcaa ttaattttag gcaaatgaaa gaggctgtga
    21541 gcctgccgtt taacaccgtt tattcgaaaa aggggagtat gacacaactg ttgtcatact
    21601 ccttttttca cctttccaaa ccaaggctca gctattcaag tagataacat tatgttatga
    21661 tagcaaagaa atgtattaag aaaacctttc actatagagg gagaaaaaaa tgaacgattt
    21721 attagcaact atatttacag gtattattat tgatgaaaat gaaaaaaact actttgtcca
    21781 aaaaaacgga caaacctttg gcttaagcaa atcagaaggt gaacacaaaa tcggtgaagc
    21841 agtggaagga ttcggctacc aaaatcaaaa acatgaaaat cggatcacta cacaaattcc
    21901 taaaagccgt atcggtcatt acgcttttgg aatcgttact gctacacgaa aagatttagg
    21961 tgcgtttgtc gatatcggtc ttccagataa agactttgtc gtatctcttg atgaattgcc
    22021 gacaatgaca gagctatggc ctaaaaaagg ggatcgcttg atgatttccc ttagagttga
    22081 tgcaaaagac cgtatttggg gcagtttagc agatgaaaaa atattcaaat ctttaagcaa
    22141 atatggaaca gaagaaatga aaaacaaaga tgtgatagga acagcatacc gcttgaaatt
    22201 aacaggtacg tacgttttaa cggaagattt ctatcttggg tttatccatc cttctgaacg
    22261 atataatgaa cctcgtctag gcgagcaaat aaaaggtcga gtgattggtg tccgaccaga
    22321 cggtgtactg aatctttcat tgaagccaag agcatatgaa gctatatcag atgatgcaca
    22381 aatgatttta accattttag aacgttctgc agatcagcaa atcaacttta cagataaatc
    22441 ggatccagaa gaaatcatga aagcttttgg tatcagcaaa ggagcattta agcgcgcatt
    22501 aggtaatctt ttgaaacaag gattgattac acaagaaaac ggtgtgacaa aactagtcaa
    22561 aaaatctaat tgagaatccc ttgcatgttt ttcttaacta gcttataata attttaatta
    22621 acatagtgtt atatcttttt ttatttgtaa ttattataaa ttgttggggt tgagtaaatg
    22681 gtcatgaatg ctatttcggc gatgaaaaaa acaaaacagc agctacatga agcggggttt
    22741 aagctgacac cgcaaagaga agctactctg cttgtgcttt tggaaaatga gaaagagcat
    22801 ttatctgcag aagaaatctt ctttttagtg aaaaaaaaga gtccggaaat tggacttgct
    22861 actgtttatc gaacccttga aatcttgaca gaattgaaaa ttgtcgataa agtaagtttt
    22921 aatgatggat tagcccgtta tgatttgaga aaagaaggcg ccaaacattt ccatcatcat
    22981 ttactatgct tgaactgcgg cacgatcgag gaagtagaag aggatctttt acttgaagtg
    23041 gagcaagtag tagaagaacg ttatcatttt ttagtaaaag atcatcggct aacgttccat
    23101 ggaatctgtc aggaatgcta tgaaaaagaa aaaagaaacc agtccgtctg agtgtctaga
    23161 cggctttttc tttgctcaga ttatggaaaa agactatcag aatgaagcgg tatctgatat
    23221 gatagagagg catgaggggg attattatga aagagcaaat caacgattat ctccactact
    23281 tgaatattga acggggattg tccgccaata cacgacaaag ctatgaacgt gatttggaac
    23341 agtacttgca ttttctacag gagcagaaaa ttgcttcatg gcaggatgtc gatcgctttt
    23401 tagtcatccg ttttctggag aagttgcatg aagaaaaaaa agcgacagca actgttactc
    23461 ggatgatctc aagtctcaga agattccatc agtttttacg gcaagagcgt atcactgatc
    23521 atgatccgat gcaacatatc gatacgccga agaaagcaca gaaattacca agtacattga
    23581 ctctgacaga agtagaaaag ctgattgaag cacctgatac gacaaaaacg ttagggataa
    23641 gagatcgtgc aatcttggaa gtcatgtatg ctacagggat gcgcgtcagt gaattgattg
    23701 gattgaaatt gggagatctt catctttctc ttggactggt acagacagtt ggaaaaggag
    23761 ataaagagcg gatcattcct ttaggtgatt atgcgatcca atggctggaa cgctacttag
    23821 acgaagcacg tccattactt gtaaaggacg catctgaaat gcatgtattt gtcaataatc
    23881 atgggaaagg tctttctcgg caaggaatct ggaaaaatct gaagcagcta gtacgagagg
    23941 ctgggattta caaagaagta acaccacata cgctgcggca tagctttgcc acacatctgc
    24001 tagaaaatgg agctgatttg cggacagtac aggaattgtt aggtcacgcg gatatctcta
    24061 cgactcagat ttatacacat atcacgaaga aaagaatgac ggatgtttat aagcagcatt
    24121 ttccaagggc ttagtcatag gaaggtgaga agatgttaac acattaccgg aaagaaaacc
    24181 gtaaaattgc tatgggacta ttgagtttcc ataagaaact gacaggtaaa acttctttgt
    24241 tgaaggagat tgatacgtac gaatctgaag acgattatga actttttttc tttacgccag
    24301 aaggttcaac caacattcaa gggattattg gtgtttactg ggcaacgaac gatcagctga
    24361 tcatccatga catttctttg aatccgtctt atcgcggtga gaatatgggc tttcaaatgt
    24421 tagatgaatt acaggaaaag tacccggatg tctccatccg tgcaactgaa atcacagagc
    24481 cttacattgg aaaatggcta gcaaagaaaa agaagaaaga gtgaatgagt tgacggagat
    24541 caatattaaa ttagatgtat tcgaaggtcc tctcgatctg ctgcttcatc tgattcagca
    24601 aatggaaatc gatatatatg atataccgat cgctacgatc acggagcaat atatgaatta
    24661 tatccataca atgaaaacac tagagctagc tgttgcagga gaatacttag tgatggctgc
    24721 tacattgatg gcaatcaaaa gcaagacatt gcttcctgtc caagaattcg tcacagatga
    24781 tgaagaatta tgggaagaag atcctcgaga agaattggtc actcagcttt tagaataccg
    24841 taaatataaa tatgcagctg aaagactgac agaaaaagca gaggaacgaa gtctttatta
    24901 cacaaaagaa cctatgaaca tcgatgggtt agcggatgct gaaaagccgt tgaagcgagg
    24961 acaagtcaaa aaaatcgatt tgatccgtgc catgcagaaa atcttagaac gtaagaaatt
    25021 ttctcaaaaa gtggaaaaaa cgatcacgcc cgatgacaca acgatcgaag aacgaatggt
    25081 atttatcgaa gagcgtataa aaagaaatgc gcatggctgt actttcgatt ctttttttga
    25141 gtcaccttct aaaagtgaag ttgtcactac ttttatggct ttgctagaat tgatgaaaaa
    25201 agggcagatc attgcgcaac aaaaagaaaa ctatgaatca attatgcttt atccagcagt
    25261 aggagatacc gaacatgacg aaattaagtg aacttgaagc ccttcttttt attgttggcg
    25321 aggaagggat cagtaatgaa gaattaagcg gactgttaaa tctcagcgaa gcagaagtca
    25381 caagtttagt gaaagaatta aaagaatctt acactcaaaa ctcgcactca gctttgcata
    25441 tttttgaaac ggaaagccat tatgtattaa cgactaaaaa agagctggct cccttactta
    25501 agcgatatgc gcaagggaca tcaaacacat tatctcaagc cgctgtcgaa accttagcaa
    25561 tcatcgctta taaacagcca atcacaagag ctgaaatcga gcagattcga ggagttcagg
    25621 tttcaggagc ggtacagcgt ctagctgccc ataaactgat tgaagaaaaa ggtagagtag
    25681 aagggccagg tcggccgata ttatatggaa caactggcta cttcttagat tattttggtt
    25741 tgaaatcatt agaagaactg cctgatacac aagaaatgga aatttcattt gaagaagacg
    25801 tgtcaccaga tctattcttt gatggaatga ataaaggaga ataattgtaa tggaaagatt
    25861 gcagaaagta attgcccacg ctggcatcac ttctcgtcga aaagcagaag aattcatcct
    25921 tgcaggacgt gtaaaagtaa acggacaagt tgtaagggaa ttagggataa aagttgggaa
    25981 acatgacact gtggaagtgg atgaagtccc aatttatcaa gaagaatacg gttattattt
    26041 attgtacaaa cctaaaggag ttatttctgc tgttgcagat gacaaaggaa gaaaagttgt
    26101 tacggatctt ttgccgatgg taaaagaacg tatctatcct gtaggtcgac tggattacga
    26161 tacttcgggg atcttgctac tgacaaatga tggagatttt gctcagcgcc tgacacatcc
    26221 aaaacatgaa gtggataaag tttacgttgc aaaggtaaaa gggatcgcta caaaatcgat
    26281 gcttgcacct ttgacaaaag gaataaagat cgaaggacga aaaacatctc cagctaaatt
    26341 ccaaatttta tcagtggatc ctaagacaaa ccacagtatc gtggaattga cgatccacga
    26401 aggacgtaat catcaagtga aaaatatgct tcaagcagtt ggtcttcctg tgcaaaaact
    26461 aaaacgtgaa aaatacggag atctgacttt acaaggccta cgtcctggag attaccgtga
    26521 actgaacaaa aaagaagtca gcagtttgct gaaccactca aaatgaacac ttacttttta
    26581 taagtgttag atgttgcgtt ttttgaatag atatgtataa tgaatatgta aacaaaaatt
    26641 atataaggtt cgtcttcagg ggcagggtga aattcccgac cggtggttat agtccacgac
    26701 ctattttgca aaaaatagct gaatcggtga aattccgata ccgacagtat agtctggata
    26761 aagaagatag agcttatttg acgacgcttg atcaaataac tctaactcta ttttccctgg
    26821 aaaatagagt tttttcattt ggagagaaac gtcaaaaagt tcgctgaaga ggagtcctaa
    26881 caggaggatt tcaaatgaag aacacccgtg tacagaaaat ggttgcagta gcgatgtttg
    26941 ctgcgatcgc gttagtttta cagtattttg cttttccgat catgccggct tttagttttt
    27001 tgaagatcga ttttagtgat atcccggtat tgatcagcat gttcttattc ggtccagcgg
    27061 ccggagttgc aacagcgttc ttgcgttcag tattacatct gctgactaca ggtttttctc
    27121 cggataattt ggtaggagac actgctagtt tcttatcaac aacgatcttt acattcccga
    27181 tttattattt ctttagtgta aagaaacagc ataaaggaag aaacaaattt ttaggtgtca
    27241 ttacaggaac catcgctcta actattttta tgagtatcgc aaattatttc gttattacgc
    27301 ctctttactt aatgttttta ggactgaacg caacacagat gctaggcatg tctttagcaa
    27361 actatgtact gattgggatc gttcctttca acttgatcaa aggattcatc gtgagcgctg
    27421 tctttttagt gctgcatgcg caacttcttc catggttaag tcgaaaacaa catcagttcg
    27481 aacaacgtca tacagcagtc aaataattat caaaaagatc aagaaatttc ttggtctttt
    27541 tttttgg
//
LOCUS       DAZUKC010000035        26664 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000035 DAZUKC010000000
VERSION     DAZUKC010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.036"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            149..1600
                     /locus_tag="ACG80T_001414"
     CDS             149..1600
                     /locus_tag="ACG80T_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS4841074.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1949..2581
                     /locus_tag="ACG80T_001415"
     CDS             1949..2581
                     /locus_tag="ACG80T_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFS4841075.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLTGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3169..4608
                     /locus_tag="ACG80T_001416"
     CDS             3169..4608
                     /locus_tag="ACG80T_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4841076.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4903..5121
                     /locus_tag="ACG80T_001417"
                     /pseudo
     CDS             4903..5121
                     /locus_tag="ACG80T_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            5370..5585
                     /locus_tag="ACG80T_001418"
     CDS             5370..5585
                     /locus_tag="ACG80T_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841077.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5802..6058)
                     /locus_tag="ACG80T_001419"
                     /pseudo
     CDS             complement(5802..6058)
                     /locus_tag="ACG80T_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6245..7156)
                     /locus_tag="ACG80T_001420"
     CDS             complement(6245..7156)
                     /locus_tag="ACG80T_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS4841078.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7175..8179)
                     /locus_tag="ACG80T_001421"
     CDS             complement(7175..8179)
                     /locus_tag="ACG80T_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFS4841079.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8176..10299)
                     /locus_tag="ACG80T_001422"
     CDS             complement(8176..10299)
                     /locus_tag="ACG80T_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFS4841080.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10304..12751)
                     /locus_tag="ACG80T_001423"
     CDS             complement(10304..12751)
                     /locus_tag="ACG80T_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4841081.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12738..13127)
                     /locus_tag="ACG80T_001424"
     CDS             complement(12738..13127)
                     /locus_tag="ACG80T_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS4841082.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(13187..13690)
                     /locus_tag="ACG80T_001425"
     CDS             complement(13187..13690)
                     /locus_tag="ACG80T_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFS4841083.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13703..13927)
                     /locus_tag="ACG80T_001426"
     CDS             complement(13703..13927)
                     /locus_tag="ACG80T_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841084.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(14031..15941)
                     /gene="ltrA"
                     /locus_tag="ACG80T_001427"
     CDS             complement(14031..15941)
                     /gene="ltrA"
                     /locus_tag="ACG80T_001427"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFS4841085.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(16687..16824)
                     /locus_tag="ACG80T_001428"
     CDS             complement(16687..16824)
                     /locus_tag="ACG80T_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFS4841086.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(16821..18005)
                     /gene="mobT"
                     /locus_tag="ACG80T_001429"
     CDS             complement(16821..18005)
                     /gene="mobT"
                     /locus_tag="ACG80T_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFS4841087.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(18264..18554)
                     /locus_tag="ACG80T_001430"
     CDS             complement(18264..18554)
                     /locus_tag="ACG80T_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841088.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(18544..19686)
                     /gene="dcm"
                     /locus_tag="ACG80T_001431"
                     /pseudo
     CDS             complement(18544..19686)
                     /gene="dcm"
                     /locus_tag="ACG80T_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(19762..19926)
                     /locus_tag="ACG80T_001432"
     CDS             complement(19762..19926)
                     /locus_tag="ACG80T_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841089.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            complement(19919..20593)
                     /locus_tag="ACG80T_001433"
     CDS             complement(19919..20593)
                     /locus_tag="ACG80T_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFS4841090.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            complement(20671..22011)
                     /locus_tag="ACG80T_001434"
     CDS             complement(20671..22011)
                     /locus_tag="ACG80T_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFS4841091.1"
                     /translation="MYKGHRIRAGDQHLVYHFVIGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            complement(22094..22429)
                     /locus_tag="ACG80T_001435"
     CDS             complement(22094..22429)
                     /locus_tag="ACG80T_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFS4841092.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(22439..22813)
                     /locus_tag="ACG80T_001436"
     CDS             complement(22439..22813)
                     /locus_tag="ACG80T_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS4841093.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(22826..23140)
                     /locus_tag="ACG80T_001437"
     CDS             complement(22826..23140)
                     /locus_tag="ACG80T_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS4841094.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(23253..26480)
                     /gene="fss3"
                     /locus_tag="ACG80T_001438"
     CDS             complement(23253..26480)
                     /gene="fss3"
                     /locus_tag="ACG80T_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFS4841095.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYCQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
BASE COUNT         7748 a         5547 c         4645 g         8724 t
ORIGIN      
        1 tttaaaggca gtaacaggat taaacattga aacataatcg ttactaaatg aaatcaagaa
       61 atatactgta ttactcaatt cattgaaaat atttatcata atttttaata gaaaacacta
      121 ttaaagagaa aaaagagagg taaatcaaat gaaaaaatct ttaaaacttg catcagcagt
      181 tatattaatg tcccaactcc ttatagcaac acctttccat gtgttggcaa gcgaaaattc
      241 aacatacaaa attgcaaaag aaagaaccga gacgactgaa atgagtaatt ccgatgtaac
      301 agcagagagt agtgaacttt tgacaacttc atcaagtgaa agtagtacga cttcaaatgc
      361 cgagtcttca acaacttcat caagtgaaag tagtacgact tcaaatgccg agtcttcaac
      421 aacttcatca agtgaaagta gtacgacttc aaatgccgag tcttcaacaa cttcgtcaag
      481 tgaaagtagt acgatttcaa gtaccgaatc ttcaacaact tcaagtagta caagtgaaat
      541 aacttcttcg aaagtgacaa aaccaaacca tatagaatct tcaaaaaagg aagcgccaaa
      601 aacggaagga caaacaaaaa ctttttcgac accacagcaa atagaatcaa cagaatcttt
      661 ccaatcacaa ccaatactat ctggtaatga agaaattcat tttgacaaaa atgaatctgt
      721 agaaaatttt attatgaaaa ttggtgagtc tgcgagaaaa attggtcaga aatatgattt
      781 atatgcttct gttatgattg cacaagcgat cttagaatct gccagtggtc aaagccagtt
      841 agctcaggca ccgaattaca atttatttgg gataaaagga acttataatg gaaactttgt
      901 cattatggta acaaacgaag atttgggtaa tggaacgctt tatacgaccc aatcaaaatt
      961 tagagtgtat gaaaactatg aggaaagctt tgaagattat gccaaattat taactaaggg
     1021 aattagtgga aataaagatt tttatgcagg agcattaaaa gctaatagta aaacatatcg
     1081 agaagcaact aaattcctta caggacggta tgcaacagat acacaatact atttgaagct
     1141 aaatgaatta attaaaacct acgatttaac caactatgat aaagaagtta aaggtccaca
     1201 aatgagtaag actggatacc aagtgcctat tcataattat tcaataacca gtttgtttgg
     1261 aaatcgtgga aatgaatttc accgaggact tgatatggca gctacacagg gtgagccgat
     1321 tcatgcagtt aaagctggta aggttgttaa agcagagttt cactactctt ggggaaatta
     1381 cgtggtgata caacacgaag atggatcaac cgcgttatat gctcaccagc aacaatacct
     1441 agtaaaaact ggagataatg ttttgcaagg acaaataatt ggctatgtag gaagtactgg
     1501 gaatagtact ggcccacacc ttcatttgga aatttgcaaa gatagtagtc tttctcagag
     1561 catgttagtt gatccgaaaa ccttattatt cggagactaa aaaaggtaat caatctgaca
     1621 aagtaacaag aaaatggttg gattcttaga cctttgatca ggatattgag actggaattg
     1681 atatctcgat tacgatgggt ttgacaaata aaacaagtcc attatcgaat atcgagtgta
     1741 ttattgccgt ctttatgctc atattttgag caggtttcac tagatgaaaa caatttatct
     1801 atcttgagga tttcgtgtac gtatcgttca gtcttgtatt gtaaattagt cataaaatca
     1861 gactaagaaa aattggaaat atttttttct gatttattaa aagttaattg aaattatatg
     1921 gtacttcttt cttgggaggg taggtgcagt gaaggaaaga tatggtcacc ttactgttgt
     1981 tgaggagatt actcataatg gatataaaaa atatcgttgc ctatgtgact gtgggaatga
     2041 aacgattgta tatcgatcaa acttactaac aggtagaacc aaaagttgtg gatgtggaaa
     2101 ttataaaaat aggcataaat ataaaaattt gacaaataaa acttttggta agttagttgc
     2161 aatcaagcca actaaactgc gaagtgaagg aactgttgtt tgggaatgtc aatgtctgtg
     2221 tgggagaaaa aagttagtat cggcaaagcg acttctaaaa ggtgatgtaa aaagctgtgg
     2281 atgtttgaaa tcaaaaaagc atcacattga aaataaaaaa ttcggattac ttactgcaaa
     2341 gtatcctttg gacaataact gttcatggaa aacaaaatgg gtttgtcatt gtgagtgtgg
     2401 cagtatgtgt gagataagtt atagtaactt attttttgga catactcaaa gttgtggatg
     2461 tttaaaaagg caagaaacaa ggacatgtgt tgaagggaca gtaatagagt gtctaacgtc
     2521 aaaattaccg aaaaataata caagtggtgt taagggagtt tatcgttcta aagagaaata
     2581 gattgcgtat ataactttaa aaaaaaagag gtactattta ggatcgtatg attctattaa
     2641 gagtgcagct caagctagaa aagatgctga aaataaattg tttcaacctt tgattgacaa
     2701 atatcgaggt aatcattaat tattgtggat aataattaac agtgatatat aaacatctat
     2761 aatatattat tcatactatt gatatttagt ataatcctat ggaattgaat atcatagcaa
     2821 tgtatttgaa aagcaaattg gaaaacaaat aggtgcaaca ccataaatat gaaaaaattg
     2881 ggaaaaagta gaatggaaat ttgcttacgt ctcacgagat agctcacact attaatctga
     2941 agaacaatga agtcgacttt tagaaattgc tagaagaaag taaagcgaat tatcactagt
     3001 tactatagag taagttcaaa taaagcatct ccaacttaca tggttacatg aggatatcaa
     3061 atcgaaatta ctcaagcttc ctatggctag agtcgcgaga agttgttggg cagtagacat
     3121 aggttatatt gttcttagct acacgatcat tagaaaggaa atcctattat gtatagttat
     3181 caagaagatg caaaagtcca aaaaaatcgc tggtggattt tgattgctat atctatgttt
     3241 accttcatgt ccacattgga tggcagtatt gtcaatattg cactacctac catctcagat
     3301 gatatgggca taccaatgaa tcaagcggaa tgggtagtgt ccgtttatct gatgattgtc
     3361 tgttcttgtt tgttgctgtt tggtaaaatt ggcgatagcc gtgggaaaat caaagtattt
     3421 cggattggta cgctgatctt tgtcttggga tcgctgttgt gtgggttcaa tcattcgcta
     3481 ggtattttat tgttcgcacg ggtggtacaa gccattggtg ctagtatgac tatggctaca
     3541 aatgccggaa tcatcacgga agtctttccc atacatgaac gtggtcgggc attgggagca
     3601 ataggtgcgt ttgtttcatt aggctcgatt gctggaccag gtcttggcgg cttgattttg
     3661 tcccaatttt cttgggcgta cattttttgg atcaatgtac cggtgggaat cgtaacgatg
     3721 ttgattgctg aaaaattctt accaaaagat atcaccatga gcggccatac aatcgatgga
     3781 gggggctttg ctgccattgc ggctacgatt atcagctttt ttggagcaat ctttttgggg
     3841 caagaaagtg gctttttcca tccagtcccc cttgtactgt tcgtaatttc agtgattgcc
     3901 tttgtcagct ttttaaaagt tgaaaagaaa gcagcctctc cattgatttc tttcggtatt
     3961 tttaagaaca agatttttac catgagcttg attacagcct tgttgatttt ttcttccaac
     4021 ttctttgtca atgtagtgat tcctttttat ctgcaaaatg ctcgcgggtt gagtcctagc
     4081 tatgctgggc tgttgatgat ggtctttcct ttgatgatgg tcgtcggggc gcctttaagc
     4141 ggctacctga ccgaccgggt tggcccggag attttggtat tagtaggctt gggcttattg
     4201 tcggtaaccc aggtcatgta tatgtttttg tcagtggact ctcctttgtg gctatacatt
     4261 gtcgctactg ccatcatggg tgccggcaat gccttgttcc agtcccccaa taatacgatg
     4321 gttatgagct cggtcactat cgaaaacttg ggactggcag gctcgatgaa ttcctttgcc
     4381 cgaaatttgg ggatggtcag cgggatttcg ctttctacga cggtcttata tcaagcgatg
     4441 tctgctaaga tgggccagca agtaactaac tatatcgcaa atcggccgga tgtcttttta
     4501 tatggaatga agattacctt tttaggcagc tttgttttgt gttttgttgc ctttttcctg
     4561 actatctggc gtatcaaaaa acaaggaggt attcagaaaa atgtctgaaa aaaccactgt
     4621 tgaagaattt ttggaggcct gcgtatgggt gagtgggatt tccatggatg atggaagatg
     4681 aagtgaactt ctaaaagccg aaaaactcaa gtattatctt caaaatacta gtgctcaatg
     4741 agatgcttga tttgatcaca agaagactgc gaaagccgct ggcaaccgca agctaggtat
     4801 cccaccgatg gactacgttt tagaggctac tggttatgaa tacggagcca acgctctcat
     4861 ctgcataaga atacatcacc cagattatgt aaagattttc gatacttctg tcaaaaaatc
     4921 attaagatag ataaatgatt tccgtctagt caaattggtt cagaatgtag acacacagat
     4981 ggtaagaagt cactcacaat tcgagaatgg acttgtccta tttgccgtat acatcataat
     5041 cgaaacgtca acgatagtat caatattttg accaagggct atgaatacaa gcattgcctt
     5101 agaaaacatc aagaacctta ggaacaacgg ggatagcttg gttaataaga gataccgctg
     5161 tttaggcttg caagtatact cttttatcaa gaattccgtg gctttagtca tgagaggctt
     5221 aatactatga aaattttctt tagcattttt gatttaggtt ccttttctaa ttataataag
     5281 atgaccacta cagtaaccta ctgaaatata taaacagcca ctgtagtacc attttcaaag
     5341 tagtctatga gaggagaatc ttcattctta tgaacgctgg aattgcggat atgaatttaa
     5401 taaaaaaaac ccttaatgat tttacttcca attcaatatc aaaaggtact ggtatcaatt
     5461 taagcacaat aaaaaagctg aaaagtggtg aaagatcagt ggaaaagctg aatcttttag
     5521 atgctataaa aataacggaa tttgctatga aaaatggaaa agctgagatt gaaatatgga
     5581 gataattatc gtctagtgaa ttacttgcca cttagctgaa tttaacgatc cttggtgctt
     5641 gaagggggag attcttggga atagagcata cttgcaagcc tatgtcttta ctaacaacga
     5701 tatctcttat taaccaagct atccccgtag ttctaaggct tttactttta tctaagcaat
     5761 aatcgtattc aaaggaaatt tttgaccctt agaaagtctc attttatcag acgtgagctt
     5821 aggcattgct gaattttgtt aattcggttt taggttgtgc tttgagcata atatgaaagt
     5881 attctttatc acgatttcat tcatctaagg taatgtgatg tgattctgaa attctttcaa
     5941 aggcagtttt tgcataagct gacattacat catcaatgac atgtttacaa tgtttcacta
     6001 ctaaaataag ggggtaacca agaagaaaaa ctgaatgatt atttgtatct aattccatgg
     6061 tatatcaacc atttcctata tagactgatt atgacatgga ttaaaataaa aaggcactat
     6121 gtcttaattg tcggttttcg agttaccgaa tggcattcat cacccaccta tagaggatgg
     6181 gcgacttctg cctgaaataa gttacactac ttataacttc taataaaatt agattgagtc
     6241 attattattt aacatcaacg attttccagt ttccattttt ttgtaatact aatttgtact
     6301 gaaagtagct agtggttttt gatacattat caaggtattt tatctttgta ctcactagaa
     6361 aattttttcc gttttgtaga taaactggat tctctaactc cgaaaacttt aagtcaatat
     6421 tgattggtcg catcgcacca ttttcaacat aatactccaa ttctttttca gaagctgatg
     6481 gataaagttt aaaaaatgtt tctaaaaaat cggtgatttc ttccgttgtt ttggcatcaa
     6541 tttctgaatc gttttctact tgttttgctt catattttgc cttgtctggc tttccaatca
     6601 ttgttggcaa actagttact aaatgattcc tgttttcatc ttcaaagatt gtcactcgat
     6661 atgcagaaga aattgattgc gtcttttttc cctctgtaat atgttgctcc actaaaaatg
     6721 aaacgacaaa ttcttccgag tgtttttcaa catctagaat ttttacagat tgaacttcgg
     6781 aactcgtggg aatatctgct cgaaccatat cttggattaa agcgagaccg ttttctgtaa
     6841 ggtactgttc gagattactc attctctttt caattgcttc tttgttattt ttccatgaaa
     6901 aatattcttt cgcaaaatcc tctgtaaaac tttcaacgcc agaagtattc actaccttct
     6961 cttttataac cactttttca tgagttgtat gttgatcaat ggcggtgaaa tttttataaa
     7021 cgccaaaagc aagactacaa gacaataaaa gccaaagggc aagcaccatt tttcgatgct
     7081 tacccacgga tacaactcgt tcttttttct tttttgggct cttttccttt tgattccgtt
     7141 caatttttat cttcattctt tttcctcttt tctcttattt gtatcggcca gctccgataa
     7201 gatgctgccg ccaatagttt tccgttagat tcgtccaccc cactggatct cctgcatgaa
     7261 acatgcgatt ctcccctgcg taaatgccca catgggtcac ataagtaccg gcatcatagg
     7321 ttgaatgaaa gaaaactaaa tcacctggtt tggcatcttt tagatcaata tgagtcatgg
     7381 catcatattg atcctgagcc acccgaggaa gtttcaggtc aactttggca taacaccatt
     7441 gggtcaagcc actacaatca aaccccgttt ctggacttga gccaccaaac acataggccg
     7501 ttccttcata cttaaatgcc tcatcaaaaa tttcttggac tattttgtca tcaaactgag
     7561 ttgtagttag gtattgcttc actaataaac agtaaaacat attgccataa ttataccgcc
     7621 acccaccatt ttccttaatc gcgattgggt tggggtaatc gactttttga ccacctgatt
     7681 tttcctttga aaaagattca gctaaagcaa aactatgctt cttcccgttt ttcccgacat
     7741 gatctagata cccgccacca tagttatagg cctgaataat actatcttga tcacacccta
     7801 gttctttgga acgcttaacc aaagacgcaa aatagagaca gccttgtttg atggatgcct
     7861 cggtacttaa tgaattaggt ggtaatccgg ctgactcaga actttgcata acgtcttcca
     7921 ttgtgccacc agattccact tgaataatcg ccaaaagaat tggaatatac tctgttatat
     7981 ccgattcttt ggcatacttt tccaccattg cacggtgttt caatacttct tctgagacac
     8041 tagttccgcc aggagaagtg ccagaactac catttccctc actgtcttca ccaaacaaca
     8101 aacccacaca catcaaaata cccataaaag agatggtcag aaaagttaat gaaataacac
     8161 ccactttctt caagttcatt ttttctcacc tgaccttgat tttggacgat ggttcattct
     8221 tttttgtact ttttgattgg tctgctttgg cagagtggac ctctgtttca cttgttggaa
     8281 agaatttttc ttacttgctg attggctttt cccttttgaa atgatttctt gactagttag
     8341 ttttcgtttg atttcgggat tcagatttgc tctcacaatc cgctttggtg cttctttttt
     8401 cagcatcata tctttctgag gttgagctgc tttatctatt ggaatattcg cccttctttt
     8461 tccctgacga tttgttgctt cactcagtat cttgcgtttt tcgtccattt tcgcatttct
     8521 tcgttgtcgt tgtttttctc gcaaatccac tcgatccgct ttttcttgga acagtccgcg
     8581 cttgaattct tctggtgctt ttttcgactt ttcgattcct ttatggagat tgtatttcaa
     8641 attcgttggc gtattcctta cctgatcctt tacttgttta gtcttatctt tcaccaaagc
     8701 ttgggtatcc aaaactttgc cgatttttct cccagccaaa ttcattcggg attgtttctt
     8761 attatttgaa actggattct ccttagttaa gtcagatagt atttcgtgat cgttcggtaa
     8821 tcgtgaattc tttcgtagag aagaaccgga tggcttaaat ttggattttg attgttcctt
     8881 tgccaactta tttccgactg ctgcaccacc accaaaagcc aaagctcgtc ccacatttcg
     8941 attgagttgc cgcaattttc gattcattaa catttgcggt tttcgcatga cgcgtcttcc
     9001 cattgactgg ctatcattac tctggaggtt gaacatactc ataatgtcgc ccaatttgaa
     9061 atagataccg gcaaaggtca caatttgcag aaaagcaacc atgaaaaatg gataggttgc
     9121 cgaaatattg aaaaacatcg tggagatgct aaatgcagtc gtaatcacca aagtaacacc
     9181 cgctcgcatc ataatcgtat tgaataaacg aataatcgcc ttctttccta aactttcata
     9241 agtcggcaac atggacaata agaaactaat tggcagaaac atcgcaaaga taatgaagag
     9301 aatttgcgag aacaacataa tccctgttag aagaaaaaca aagaaggaaa tgccaatatt
     9361 gaacaatacc aaaaagacca cggtccctaa acggttcatg gtcttggtaa tactgagatt
     9421 cgcattgtca ttgtcttcaa tctctgcctt gaccgcttct tcccgatctt tgcctttgtt
     9481 ttcatctgga ctcacagata gaatcttgtt gacccggtct tcaccaattt cttctatgtt
     9541 ggaatcatca aattgcagta gtaaccaagg ttgttggact tgaatcgaaa acaaactatc
     9601 ccgaatcaaa tccacactat cttttccttg gctttctgaa ttgggtacaa cgatctcggt
     9661 tccaagggta agggccgctt cactaacatc tgagctaaag tcattgattt ttgtgatata
     9721 ggtaggcgca taagcaatga acgaacccga tagtagaaaa atcatcacaa aattaatcac
     9781 ggctcgaact gctttcgtgg tttctcgttt taataatccg gtatacgcca catacacccc
     9841 cagagctaaa atcatcaata atagaaaccc aaaataaaat ccggaggtct ggagtccatt
     9901 ttcagtaata ccagccaatg tttggatatt ctttccaata ctttcagccg tatccgaaat
     9961 gaaatctaat ttgtaagcct cttgaaccac ataacccgtg gcgttcgaca aatacaaact
    10021 caccgtccaa atgaaattag taatggcata aagaccatac atcacgcttt tccctaagcc
    10081 atcaccccaa ttccaaggca accaatccca agagctatcc acaaagaaat ctagttgata
    10141 gttgttcagt gaatatttgg aatatagatt tcctgcttca actgtatcat caactagccc
    10201 tgtcgcctcg gctaccgtac caactaaact caatagaact atcagagtga gtatgctaat
    10261 aaaaacgatt cccacaatcc gaaaaagttt ctttttcatc ccctcactcc attctctctt
    10321 gaacaggtgg acgggtatca aaggcatgga agagttcttc aaagactgga tgtacctgaa
    10381 ccacgcctac ccgaccatac aaatcctgca tcaagcattg gccattttcc aaatctcgta
    10441 ggcgtttttg attgctttca tcttctcgat ccaagccaaa gaattccaag gttttcttga
    10501 tttcatgaat atcggtactg cgaaacgcaa acttcaaccc gatattattc ttcaagcgtt
    10561 catccagtaa atcatcacta ttttgcgtga caaagtaaac cccggcattc atagcccgcc
    10621 cagcacgaac gagcttattc gataatgttt tcccttgcgc tacttgaaga aaactccacg
    10681 cctcatctaa gtctacaatt ttgaagattt ctcgattact atggataaag tccaaggcaa
    10741 aagtggaaat gacgattagc atggaaacac taagcaattc catggtggta tattcttcaa
    10801 aagtggtttc tgcatctggt aaaaccaaat ccgctacttg aatgatattt aactgcctat
    10861 ctaaactaat cgactgggta atcgaaccat ctgagaaaag caaatgggca aaatcataat
    10921 cggtaaagga ctcgatgtga tccgcgatat tttccgcaac tggtgagcct tcttttcgaa
    10981 gctcattaat tacttgtaat aatccccgtt gattgctttg ggtaaccgtc cgaatggcct
    11041 tacgcaacac tggaaacttc tccccatctc gactagaaat tcctgttaaa aaggtcagaa
    11101 tatcaattgc tagactttca gaatccttct ttttctccat aatcacataa ggatcaagta
    11161 atccttgatt ttttggctta ctagttaagt tgagaatatt gatttcatcc gcaatttctg
    11221 gcaaggtttc tttccagcca ccccgttccc cttttggatc gacaatcaca gcttgtccac
    11281 caaaaaggac ggcgtaataa actaagagat tgttactaaa ggactttccg ccaccgagtg
    11341 aaccgaggaa tgccgctgct agcgcattgg taactgaatc cttgacccct tgtgaggcca
    11401 atgccggttt caggtaaaca ttgcgccccg tatctacgtt ataaccaaag taaatgcctt
    11461 ccaactcccc taacatctga gtggcgccaa aaccaagtcc ggcgagaaaa tcagacgtaa
    11521 catactggat ataatcattc atgtatcgct tactagaagg taagaattct tcatgtagcc
    11581 ccatcatatc gccaaaaggc cggactagtt ttacattggt atcatcataa aaatctagca
    11641 cttcatcaca ccggcgcttt agttcatcta ctgattcggc acttactcga accacatagc
    11701 tcagtttgta catggattct ttggactgat ccaaggtagt ttccaattca tctacggaat
    11761 ccaatgcatc taagacattg gaatttgttt cattgtcaga ttgataggca tggttgtcca
    11821 agtcttttaa ttcctttttc ttattgcgaa cggtagcaag tgcctttttg ttcgtcacaa
    11881 tctccacatt catactagtg tcaattggga atgtaaattg ttgttgctgg taatagaaaa
    11941 gttctgagga gggaaactcc atttccccca caattgtatt aattgttaag tacgctacat
    12001 atgattcatg attctcatgt tccatgcata agtaccgggg cttttcttca atcaaacaac
    12061 gactgggacg taataagtcg taacgtttca ccaaagtttc cgacttcagc tttttctttg
    12121 gtagctgaaa ttcatattct tcaaatggtg tacccttttc gccatagata tgttcaatca
    12181 aatacatcag atcactcggt gtgactcgtc tgactttaaa tcttcgagcc aatttacttt
    12241 ccattagttt ttcaagcttg gtatatcggc gaatttcttc attggataag gaaacaaaat
    12301 cgcccatcaa atgatgattc actccataga caaattcttg aaagccggag aagaaagatt
    12361 tcttgagatt ctttagattg acctcttcat ctgtcgcaat cagtttgaag ccaataaaaa
    12421 agcgataatc aatctgacta tcgccaatca tggatactaa tgcctctgtc tgtaaatcaa
    12481 ttcgttgttt cgctacttct gttaatcgcc cggtaatttc cttttttgat cgttcttgcg
    12541 tggcacgaat actactttct gtggcaattt gtaaggcatg aatcttgcct tcacgatttt
    12601 gcgcaatcaa ttgacgaaag ttatcgtgga cttgatattt ctgttcaggt gacaaaaagg
    12661 agtaattgta aggtattaac tcatagtagg caaaacattc tccctcttga ttaaagacaa
    12721 gattattttc aatatactta atcgggtaca tagttcacac tccttaccag agtaatgggt
    12781 tcgtcccatc gtttcttctg ttctttgact ttctttcctg cgaaagtgac cttaaatcgt
    12841 aagtgatagc taataaaaga tcgtaaaaat cccaatggtt tcttaccgtc aaacgttttt
    12901 tgcgacatga accaagtaac tgccaccggt atacctaagt attttaaaaa ggccccctct
    12961 atcatagata gtggaggcac atgggcaaat acgatgacaa acagcaagga taaaacaaac
    13021 caagccatct ggttaaaagt cacaggaaaa ggcagttgaa agtcattaat tgcataaatg
    13081 accttttcaa ctgcccaaat actggtgtag ctttttattt tcttcatcaa atcattcctt
    13141 taaaaagaag gatagtttag aaatcaacta cccttcaata attttgttaa tatggatact
    13201 caaaaacacc tcgactcgtt accaaataat tgcctccaat ctccaagtct ctaccacacg
    13261 catcaaaatc aatatacgtc ttcaagtgtt ctggtacttc tccaaaatca ccagcttcta
    13321 caatcaagaa atatgctaca tcactcatat catcgcaatc tggataatag ataatatcat
    13381 ccacatgctc taccatctcc tcaaaagaat tgaagaaagc atgttgaatc tcagaagcaa
    13441 cttcaccaac tggcgttcct tcaagttcct ccgctaagcc acacaaccga ttaatttcct
    13501 caattggcgt atactcatca atctcaaagg gaagttcata atcatgaatt gcatattcct
    13561 cgtattcgtc atttaaccca attcgttctt tcacttcatc aaaatcgaca ggtggggtaa
    13621 accacgcccc caccaattca ccttcgttat atttcccgag gttcgcaata taaacacgca
    13681 tctgttccat cgtgcttccc ccttacgcac cgacaattcg gttaaacaaa ctgagtaaga
    13741 catccttcac cccagaagca ttaaagacca atccgaccgc aatcaaagca atcaccaaaa
    13801 agccaatcag tttggaaaac tcccgcttaa atcctaaaaa caaaccaatg accacaattg
    13861 ccagtaagac taaggattgg gcattggtta aaaaccattg atatatggat aggtaagttc
    13921 ctgatattat aaatatctca aacttttccc cccattcaca cggagcgtgc gactttcacc
    13981 gcactccgcg ttccatcgtg tttatattga aaagtattta tgtagtagac ctatatcaag
    14041 tttaattctt gattgccagc taatactcta tatttattta gtcttatgag ctggacttta
    14101 tttagcttca atagagctat atatttttgt attgtttctt ctgttttagc gtgtattaga
    14161 cggtgtaccg cctcttttac caaaataaga tttttataat cgtcatttcc accagctcca
    14221 gttggtacaa tatgatgaca atgtatttca ttagcttgtt gaaattcttc cagagttata
    14281 gcacattttc catgttgagc agaaaatagc gaaattcgat tgtctgcgta ttctattgtg
    14341 cgattttcca aagataattt cattaactgg tacatcagta atttatcaac acttagattg
    14401 gtgtggataa attttcttcc ctctagagtg tagcaattcg ctaaatacac ctttgccatt
    14461 ggttttctgt gttggatata tccaacaggg aaaataggtt cgccctcgcc agcaacatac
    14521 cgaagcattt tagatttacc atatcttgtt gcctcctttg tcgtaagttg tctgcctgtt
    14581 tttctcaatc tgcccctttt gtttgtacct aatctgttag tgaagatagc cattacagct
    14641 ctattcagtt tcatacaatc cagatttaca ttcgttgcaa tctgataata attttgcata
    14701 cctaatacca ttgaattata aagtcttgtt tcttctaatt ccgttttccc ttttgaggga
    14761 gaagcaattt tctttgcctg tgataccagc ttgtcttttt ggtttttgaa ttgtttatca
    14821 cttatatgtg atttaacaac taacttcttt cttctcggta tcattttaat cttgaaacca
    14881 agaaagtcgg agtatctttt tctaacattt acaattcttg ttttttcttg tgatacttcc
    14941 aattttaatc gttgttcaat ccattgagtg attgctattt tggctttttc agcactttcc
    15001 ttgtagcggc agaaaactct aaaatcatct gcataacgaa ccatatacat ttcctttaac
    15061 tttgttttct tcatttcttt gtaagcgttg cttttacatg ggctaccgct tttattgata
    15121 tgaaccacat agttttttgt aacaggattt tcctgccatt ggctttctac ccaatggtct
    15181 aactcattaa gaacaatgtt tgctagtagt ggggatatta ttcccccttg cggagttcct
    15241 ttctctggat atgttattgt accgttagtc attttgattg gagcttttaa tatccttttt
    15301 aaaatgaata tcaagtgctt gtcccgtatt cccatagccc atatctgttt tatcagcttg
    15361 gaatggttca cgttgtcaaa gaaaccctta atatcaaatt caatcacata atgtagctgg
    15421 ctgatttgta ggcggttata ggttgctttt attgcatttt caactgacct attaggtcta
    15481 aaaccatagc tgttttcact aaatttagct tcgcagattg gttctaatac ttgttttata
    15541 cattgttgta taagcctgtc ccacatacaa ggaataccta gaggtctggt tttaccattt
    15601 ggttttggta tttcttttcg cctcactggc ttaggtcgat acccatgtgg actgcctgca
    15661 acaatgtatc ttactttttt gacaactgtt tctgctggca atttgccaat atctttaatt
    15721 attgtaccgt ctgttcctgc tgttttactg cctgcatttg ctttgatatt tcgatatgcc
    15781 agtaatatat tttctctcga taggataata tccataagat gagtgaaaat atctccattc
    15841 ttacttttgg catataaatc atcaaaggtg ttttgcattt cgtaatattc agcgtgtctt
    15901 aaatcttcta cacacaatgt ttcattttta tttttaggca taaggcttca ctcctttact
    15961 tgaaattgag tgagcttttt tatcttactc gtaatcctta ttgaaacaac ataatacttt
    16021 tctaatatat acacgacttg agggcattcc tccattctca ttacaagaat ttcttaggtt
    16081 cgccctctgc ttttcagcat tagttcaact gcttattgtt cttcgtcagt ttattctctt
    16141 gatgagaacc caataccttt cctcgttccg ataatcttat ctatacatat atatccttag
    16201 gtcactagct ataagcctgc tggcttgata ccgcctgtaa cgttatatgg catttcataa
    16261 cctatattct tactttgcca caccagctac gatttctcgt acctttgcat ttctacaaag
    16321 ttcaaattta gacccgtaca ttcgctgttt cgtcagttcc ttatagaaca ttctaaccat
    16381 agatatttta tagacccccg acctgttgga tacgctaccc acctctggat agttttcgat
    16441 agaagatata tgttcatcaa ctattacggt atctttcagc cgtctttacc gagcttcaaa
    16501 cattctgagt ttcctcatac aatgcttgtc ggaggctcag ggcaggcgtt tcagagcgtt
    16561 acttcatcat tccacctgtc agattatcag ttctcctaat aaccaaactt tttcgttctt
    16621 ttattagtgc cttagctttt cactaaggaa cgagtcgcac ataaattttg tccgaaattc
    16681 atcttgtcac tttcctttct tttccattgc tcgatccgct gctgcacttg cattcatcaa
    16741 gcacatcaca gtaacaccaa gcatactgcc accaaatacc aaaaaaatat cttttacaat
    16801 tcccatgacg attcctcttt tcatataatc aaattttcaa ttgccatact ttgttgctcc
    16861 aaaatctttt tatgtcggtc tgataagcgt gcattctgta ccatatcctg gatataggtt
    16921 gtccgattca atcgatccaa ctccttagcc attttccacg taggggcgac ttgtcggcct
    16981 agccaattca aggttcgttc aatggtatac ggttttggct cggaagttaa ttgaatctct
    17041 ttccgattcc gtccaataaa acgcccccat tgttcattca agggccagtt ggttcgcctt
    17101 ttaccttcta ctttatccgc aaatcgcaag taacgattga taatggaaaa cgtggtgctc
    17161 tcaatgctac gaccttttaa caaatcttga atcgcatgaa aagcacggtc atttttcagt
    17221 cgaatctcaa aacgattttt cgtctcagtt tggtctaatg gaatccctaa cttgatatat
    17281 tgttcatagt ctttttcata caaacagaaa taaacctcac tcttgagact gccgatatat
    17341 aaggtattgc ccatgccgtc cttttcatca gcctttaaaa gttccccgga acgataactt
    17401 ttaaaggtac gaaacaagga gatacattct tccttctgac attttttcgt tagctcaggt
    17461 atatccaata gccccactcg atcattgatt gccaagtcca agcgtttcat cacgccaccg
    17521 gcctttagac aatcctcgaa aaagtcgtac cagctacgct tttgagcgag taaaaaagtt
    17581 tcaaattgtc ggcaacctcg cccctttaat tctaaaagca cacctttatc ttcttcatga
    17641 gacaacatca ccacaatatc acccatgaca tactgttctt gataggagta aaaggcataa
    17701 tcttcatgga gcatatactc aaatttcaaa tggagaattt cttgaataat ttttcgtgca
    17761 tccgttgtgg gaaagcgaat ccttacatag tcaaacagta acgtcaaagg caaatcggga
    17821 ttaaagcttt ccagttggtc aaaaattttc ctcatggttt ttatagatgc ggttacgcca
    17881 ccattttcta tctgatttaa gtacggccga ctaagtccac aagccgtagc caatttattt
    17941 tgtgaaacgc cgtattcgtt gcgtcggtct tttaatagcc gataatctaa attccgttgt
    18001 gccaagttct cacctccaaa ttcaaggtgt taactttttc aaatgagtta acacctatac
    18061 aaattactta ctatctttga tacaacaagc attcttaatg gttgcttact tattttttgt
    18121 gtttttgtac ccccctgtta gatacggggg tgagacatca tggtcggcta acgccgtcca
    18181 tgaacaggca cagcaccacg gaggatttct gcttcgcgaa tcctccgcgg tcagcgccta
    18241 acagtcagtt gtgttggttt aggttactca tctgtaatac catttacttc caactctgct
    18301 ttcatttcta acaagtattg acgaatatct ggttcttttg cttggtccgc aatccgctca
    18361 aattctgcaa ttacttgatt tatttcaatt cgagcatccg tctcctcatt cattaaatat
    18421 ggatcgccag cgactttgac accattcttg cgaatatcaa attttaaccg tcgcaattgt
    18481 tcttctaatt ctgggtctga ctgaatcttt ccaatcaccc aataaatttc tccaatcaac
    18541 tcatcataaa ccatgtacat ccaactcttt taatcttctg gcaatatcaa aaatgaccgg
    18601 aactgttaca gagtttccgg cttgcttata caactgacta tctgaattga cttgagaatc
    18661 tcgatcaaaa gcccaatcgg gaaagccctg aagtcgccaa cactctctag gtgttagctt
    18721 ccgaatttga tactgatccg tgacgatcgc ttgttggtct cctgttagta aggtatgaac
    18781 actctgtttt cccagacgac ctcggcgact tgtggaatta ggaaaagcca gattcacact
    18841 gtcttctggc ttcacctctt gatattctgt ttcatttttt cgaatcatga tttttgtacc
    18901 accttgtttc gttgacaagg tgggggcggt tccagtaact tcataaactc gattaccttg
    18961 ttcaaacttt cccggtaata gtcctgctac agagattcca tcccttgctg gcagtgcgat
    19021 tttgattcct tcatcacctg ttaaggttgg cgctacgcca tctgaaccat agacttgtcc
    19081 acccattcct cgattggaag gattgatatt taccaaattt ctgatgtttt gatcagatgt
    19141 tgaagacggt ccaatgatag gaaatacttg ttcggtacat gcgtctcgag aatgtccgat
    19201 aaggaatatc cgctcccggc tttggggaat gtaggccttt gagttaagca cctaccactc
    19261 cacgtcatac cccaatccat ccagcgttcg gaggatcgtc tcgaacgtag cccctccttc
    19321 gtgattgagc aatcctcgga cgttctcaag gaataaaagc ttaggtcgga gaagagtggt
    19381 gaaccgagcg atttcaaaga ataaagtacc tcgagtatcg gaaaatcttt gccgctttcc
    19441 cgcaattgaa aaagcttggc acggaaatcc gccgcaaagg atgtccactg gtccgaggga
    19501 ttgaataaac tcatctgata cacttgtgat gtcatgcatt tccacctcct tacttgtatc
    19561 atgaattgct ttataactac gtctagcaaa tttatcaatt tcacaaaaac cgatacaatg
    19621 atggctcgcc tgttccatac ctaaacgaaa gccaccaata ccggcgaata aatctaaaaa
    19681 tctcatacat tacctcattc attgcaaaga aaaaagccaa gaacattttg ctcttggctc
    19741 atacattatt aatttgtcct actatttttt atttaatcca actaatttcc gttgtttttc
    19801 attggcctga tcggctacat atttaatcac cgttttgaac tcttttgcag tagcattttc
    19861 cgataaatcc gcaatgctct tattgtattt ctttacaatt ttcgttactt gttctttctc
    19921 agtcatgtaa ttgaacctcc aaatctttcc tactataccc tagtggtaaa aataattcta
    19981 gattatccgt agcataatga acttcggaaa tattattcgt ataatctttt attatgatat
    20041 gaagcaatac atctggatgt ttactcttta cttttgcaat gttctctctt gatttgaaaa
    20101 aagcgttaat aaatgccttt ttcggcacat gacgtccctc tgcaatttca cgttcttgtg
    20161 taaatttcca agcgatttta gggtcttgat aaacatagaa aatcgtcact cgaaaatgtt
    20221 ttttcaacgc ccgcgagata tttttttcag cacttaatat tgcaaaagtt gcatccagaa
    20281 taaaagaata gccatgatct aagacatatc taaatgcctg ctctaccaac caagaagatg
    20341 ctttttgaaa gagactagag ttttttccat tataatcagg aaactgggtg cgaaagtaat
    20401 ccgcatcaat aatcacatgg ttatcataat tctcagctaa tccaatggca acctctgatt
    20461 ttccagcccc aggactccct gccataaaaa ctgcgtctcg tccaacttcg ggggatttcc
    20521 ctttgataat ataagaaata aacttatcct tatttgcttt tgcatactct aaataagaat
    20581 cattactttc catattaatt atcccctttc ttcctactat ataaaagtat tatacgttac
    20641 ttgaagtata aagtcagtat tgaagatcac tcaatcattc atcttttcta atttactaat
    20701 tgaatctaag aaatcatacc cttttggtac aagtggcgta taaaactcac taatcacact
    20761 gccacctgta tccacataac ctcgaccttt gatgtgcttc ataaagaagt ttttttcgac
    20821 ctccccaaac atcatggaat atccgagttc actcatacga cccaaagcca cgcgaaagtt
    20881 gaattgatcg cggattccgt ctcctaaata tttcgcatcc ggcctttggc aagccaaaat
    20941 cagaaagaaa ccagattgac gacccaacat cacgatttgt ttgagcttat tcaaaatcac
    21001 cgcactttct ttcgtcgtta acatctccat ataagccacg tattcatcaa agattaaaaa
    21061 gttgggtgga agtccaagat aggcataatt ctctcctgtt ttgtagttag acatttcttt
    21121 cattgccttg ctacgagcca tcatacgttc atagaaatct tccacacaag cagaaatttc
    21181 ttccttttgt gaataaacat gaggcatcac cgtacctaaa tcagctaaat ctgcattttt
    21241 gggatcgagg ataaacaatt ctgcatctga cttcaatagt gcttcgataa tggtgaggag
    21301 gaaataggtc ttccctccac ccgtgccacc agcgattaac atatggggta aagaatcata
    21361 agcccatact tgatttttca tcaatcgcag agtcccattt tctgccacta cttcgtctat
    21421 cccaattcga ttggcgatca tatcatacag taaagtatac tccacatagg agtctttgag
    21481 ttccttttct actaactcac aatacagtcc gctttctaac ttcttttcca attttaaaag
    21541 ctgatcttgg tatttcccaa gtgaaatttg aacgcgaata gacagtaatc catctttcat
    21601 tcgataacag attttgggga agtaactgat agtctctctg gttcggctac tattcaaatc
    21661 tttgaaaaag ccttcacttt gagtttgttt cacttcatac caatggttct ctagaatcat
    21721 ccgagctagc ttttgtcgat gccacaagct tcgccaatga tcaagaaaaa agtgaatata
    21781 aagcaatatc atagctcctg aaaaagcgag acttccaagc agacatacta aatcttttat
    21841 agaccaaacg atttcaatag tagaaagtga tagcttagaa atatcaaatt gtctaaactc
    21901 ttgaaaataa aagacgctca tccaaccgat gaacagagcg agtagccagc caataacaaa
    21961 gtgataaacc aagtgctgat ctcctgcgcg tatgcgatgt cctttgtaca ttttgatggt
    22021 ttccacctcc aatcgaaaaa gcccaatagg atttcattag gcaatttcaa aaagttatgc
    22081 tagtcattta ctgctacgag gaggggagtt gtttcgtttc aaccaattga tttaaaaagc
    22141 cttcaatagc atccggcatc tccacaccaa tttcttccaa aatggattct gcctgccgac
    22201 ataagttttc atttacttcg acaactataa agaaagactc ttcttctcct aaccagtcta
    22261 gtacatcaga gtcttcttca ctgtcgctat tttctccaag taaaccttga tgtataatca
    22321 gaaacatttt ttcatcagag gttaatgctt taacgagttg ttcctcagtc agctcgcctt
    22381 ttccatattg atcgagacaa tctaaaatat gttcaacatt aatttccata atcttcacct
    22441 atttctttct ttcattttgg ttcgtaggat ttccttgatt cggctgccgc ttcaagacta
    22501 aatcatccgc cttaatgaac caattaacgg tagttccttc tcgaaaaaca taattggcaa
    22561 cggtatccac gattggattg accaactcta cctctgcatc ataagcaaac tcgcgtaagg
    22621 gtacattagc tgggatacta acttgaatca ttcgcccttg tgcagaggat tttaaattat
    22681 aggttcgttc ttttacctca ttaccaattg ttccatcctc attttgcttc cgtacttctc
    22741 ggcgtaatgc ggaaaaacgc aacttcccaa atgttaaacc tgaatctacg acaatacctt
    22801 ctgttaatct catcttcttt tcctcctatg ctttcactaa atcatcaaca tacaacacgt
    22861 aatcagtaaa cccacgattc tcaatctcat agcctttggc aaccaaatgg ggattcacgg
    22921 cttttagcgg ttggtcttga tcaaaatcct tttcaccggc ttccgcagct actaccacct
    22981 caatatcatc ggcccgttgc ttactggaat acaaatgata actgcgacca atcaccgtag
    23041 tattccgtcc atatccttct gttataattt ctccttcacc ggcataactt aactttccaa
    23101 aggtttcagc catatctggc acgacaaatt tcaattccat cttcttttcc tcttttcatt
    23161 gttagttttg gatacaaaaa tagcgcgact acttttttag gaggcggtcg cgcacgttgg
    23221 ttcttaggat ttacttgtat catctcatca ccttagttcc ggcgattcca gaaataatag
    23281 cccgccgtcg caaaaatcaa agtaaaacca atgaacagta aaacattgga atttttctcc
    23341 ccggtttgag ggaaggtctt cactgtggga ctagattctt gagagccact tggaacagtt
    23401 ggtgtttccg gttgtttcgg cgtacttagt atggttggaa cttcttttgg tgttaaggtt
    23461 tgagattttt ctttcaaatc ttcattgtgt tttccattga tattcccatc tttatcgtaa
    23521 ttgatttccg caaaagtaaa cttggttcct tctggatatt ttccggtatc tacttttttc
    23581 gcaagcacag ttttggtgaa ttctttatca tttacttcat aatcaatctt gccagatttc
    23641 caaatttctt tgtttgtacc atccggtaat aaagcataca gaattgtttc gaaagcttct
    23701 tttgagccat ccagtacatc atgagtaatc gacacatcat caaacatatc caccacatca
    23761 ccatgagtaa aggtctgaga accgttttct aggtgggcct ttgtttggat agaaacatgg
    23821 tgttccactg tacaactgac agtttgggct tggttattca agctaacatc cttcgctact
    23881 ggcttatcgc ctgcttcaaa agcttcctta ttttcataca catagttgaa taagacaata
    23941 gttttattta aaacttgttc ggcggttaat ttatgctgaa ttttccaagt tcctgttttc
    24001 tctttgttcg ccaacgttgc gcttgtttct gcaatcacca ctggtttggc tttttcatct
    24061 ttttcttggg cagcttttgt cgcatccaca tcaatggctt gcgctaccac ataccagtct
    24121 tctttgattt catgtagatt gtaactcaat ttatcaacta gctcggtaga atccagagaa
    24181 gttaattctt tgtttccgtc tttccaagtc gcaagagaag ttaaactatc ttcttcctca
    24241 ggcaaatcat agagcattaa acggttcaaa ctaacagaaa atgctttgtt cgtcagcttc
    24301 tcgtaaggaa cggtcaccgt tttaattggt tgtttttggt cttgttcggt aatggtaaag
    24361 acatactcac tcttcacaaa gtcttctttg ttttccttaa aagtagaacg gatttctaat
    24421 ggcgtaatct tttgaaaacc ttctggggct tctacttctt caagtaaata atccccataa
    24481 ggaagatttt caaacttgcc ataaccatca aaccctaatt gctcgttata agctgtggtc
    24541 gcttcatctt cggcacctgt gatttcattg gtcccttcca atggcgacac tttaaaggtc
    24601 agatcattga agccagtttc ggtagtgccc gcagctgatc cagcaaattt aaagaaatca
    24661 aagccaaagc gaatcacttg ttcttttgcc gtaacatttc gggtaatcac ggcatttttt
    24721 tcgctatcat ccaccttttt gatggataca ggatactttg tttcatcaag gctatatcct
    24781 tcgggcgctt ttgtttcttg gcaataatac tcgttgatag ctaaatgttt aacggcaact
    24841 tggttctttt cgtctaaagt caaagtgacc gtttcatcag aacctttcgt tcccttcgct
    24901 aactctggtt taaaagcctc actccattta acagcttttc catccttagc agtaaacaag
    24961 gtgtattcag caccctcaaa gactgcctta ccttgtgctt tatccccagt atctttgtct
    25021 tctttcgtca aagtggtttc gccagtgact tcttgatttt gcccttttac gttactggta
    25081 acaggggcta cggtttgatt ggcatacttc aattcgactt ttacaggttt gaacgtattc
    25141 acgaacccat gactagcttt agtttcggtc acataatagg ttcccaattc caacgcatta
    25201 gcaatctctt ttggtgtttc cgcatgaccg ttttcatccg tggtcatttc ttgaacgatt
    25261 tcaccggttg gactgtcttt gcgaatggca aacgtattac cagctaagga ataattgtca
    25321 ttccaaagat cactccctgt ttcaactccg cttttatcca gaatgatttg ccctttttcc
    25381 cgtgcgtttt ttgaagtcac gtaaatggtt tcgcctgctt taatggtggt agtcatgggg
    25441 gtggtatcaa tcgtataagg cgccggtact gatttttcgg taatggttac ttttgtacca
    25501 tgaggaatct catccaatgt agcaatgcct tctttgtcag tagtcacgtc ttttgcaggt
    25561 aaagtttttc caaagtctaa atggaaaacc gttcctggta caacatcacc cgattcttta
    25621 tcgacttttt tgatctttaa agaaccctcc gtttccacat caattttgat tgtgtaggta
    25681 ttaggtttat caatcgcccc ggccattaaa gtctgttggc cagccttttt ataggcgaca
    25741 ggtgttccgg taccagcaga tttcttaagc gtcagcacac cactttcttt tgaattggca
    25801 tttggggtga ttatcaactg attaccattc acacgataat caatgtttgc ggcattttcc
    25861 acaacctcat cgaactctga caaattcaga ttattcgtat ccgtcacagt agtcgattgc
    25921 cctaatactg ttttagccgt actattatgg aaggttggtt tcttctgata atcgtcaatg
    25981 gcttgattta tttttgcctc aatggctgcg atatttacgg cactaccatc tgatcgtttt
    26041 attgagtgaa gggtataccc attaacttct tgccagatca tcttctgtgc cacaatatga
    26101 gtatctgcat ctgtacctac ttttttccat aaaacggaaa ctaatttggc tttatcggac
    26161 atatctggta acgggttttt ctcatagcca ggagtgaact cattgtaaat cgggactcct
    26221 ggttcaatac agaaaatatc ttgtttaaag ccgttgtaca ctgcataatg cggaacagtg
    26281 acttctgaca ttggacgacc atcactaaaa gttccttcaa cagcatattg atgtgctaaa
    26341 tctagttcta cggttaccgt ttgtggatgg gtaatttcat cagcaaaagt aatcgtagga
    26401 gcaattgctc caacaattgc ttgaacagcc aaactcaaaa tagttaatac acataatttc
    26461 tttttttgtt ttttatacat gaaatgttct cctttcaaat taagcacaaa aaaataagat
    26521 gctcgcttga acatctttgt ttataaagtc tttttaaaat ttttattgac tgaatagaat
    26581 ggatttgaga atgaatgagc ctaaaaattt aatctaaaat acgaaatata ctgcctttaa
    26641 attagaacac attttagaac acaa
//
LOCUS       DAZUKC010000036        26620 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000036 DAZUKC010000000
VERSION     DAZUKC010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.037"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(18..716)
                     /locus_tag="ACG80T_001439"
                     /pseudo
     CDS             complement(18..716)
                     /locus_tag="ACG80T_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            795..971
                     /locus_tag="ACG80T_001440"
                     /pseudo
     CDS             795..971
                     /locus_tag="ACG80T_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1147..1716)
                     /locus_tag="ACG80T_001441"
     CDS             complement(1147..1716)
                     /locus_tag="ACG80T_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS4841096.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYKTLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1790..3079)
                     /locus_tag="ACG80T_001442"
     CDS             complement(1790..3079)
                     /locus_tag="ACG80T_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841097.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFYFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLIKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQALAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            3406..4281
                     /locus_tag="ACG80T_001443"
     CDS             3406..4281
                     /locus_tag="ACG80T_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4841098.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            4295..4618
                     /locus_tag="ACG80T_001444"
     CDS             4295..4618
                     /locus_tag="ACG80T_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841099.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            4615..5367
                     /locus_tag="ACG80T_001445"
     CDS             4615..5367
                     /locus_tag="ACG80T_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFS4841100.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTAYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5357..7528
                     /locus_tag="ACG80T_001446"
                     /pseudo
     CDS             5357..7528
                     /locus_tag="ACG80T_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7640..9811)
                     /gene="copB"
                     /locus_tag="ACG80T_001447"
     CDS             complement(7640..9811)
                     /gene="copB"
                     /locus_tag="ACG80T_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFS4841101.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHVGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9831..12017)
                     /locus_tag="ACG80T_001448"
     CDS             complement(9831..12017)
                     /locus_tag="ACG80T_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS4841102.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(12017..12226)
                     /gene="copZ"
                     /locus_tag="ACG80T_001449"
     CDS             complement(12017..12226)
                     /gene="copZ"
                     /locus_tag="ACG80T_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFS4841103.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQFEKICQAINGLGYKAEVI"
     gene            complement(12239..12679)
                     /locus_tag="ACG80T_001450"
     CDS             complement(12239..12679)
                     /locus_tag="ACG80T_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFS4841104.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12753..13292)
                     /locus_tag="ACG80T_001451"
     CDS             complement(12753..13292)
                     /locus_tag="ACG80T_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HFS4841105.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(13907..15373)
                     /locus_tag="ACG80T_001452"
     CDS             complement(13907..15373)
                     /locus_tag="ACG80T_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS4841106.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(15684..17765)
                     /locus_tag="ACG80T_001453"
     CDS             complement(15684..17765)
                     /locus_tag="ACG80T_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS4841107.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            complement(18290..18649)
                     /locus_tag="ACG80T_001454"
     CDS             complement(18290..18649)
                     /locus_tag="ACG80T_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFS4841108.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGISLIVNIIWLIISGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(18679..19020)
                     /locus_tag="ACG80T_001455"
     CDS             complement(18679..19020)
                     /locus_tag="ACG80T_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841109.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSFFAQF"
     gene            complement(19017..19691)
                     /locus_tag="ACG80T_001456"
     CDS             complement(19017..19691)
                     /locus_tag="ACG80T_001456"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFS4841110.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLIAQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(20621..21919)
                     /gene="asnS"
                     /locus_tag="ACG80T_001457"
     CDS             complement(20621..21919)
                     /gene="asnS"
                     /locus_tag="ACG80T_001457"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFS4841111.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(21959..23149)
                     /locus_tag="ACG80T_001458"
     CDS             complement(21959..23149)
                     /locus_tag="ACG80T_001458"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFS4841112.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(23170..23697)
                     /locus_tag="ACG80T_001459"
     CDS             complement(23170..23697)
                     /locus_tag="ACG80T_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFS4841113.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(23744..26533)
                     /locus_tag="ACG80T_001460"
     CDS             complement(23744..26533)
                     /locus_tag="ACG80T_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFS4841114.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
BASE COUNT         7446 a         5661 c         4693 g         8820 t
ORIGIN      
        1 aaagaggggg ggatttttta tcgttgtgga atatttaaat accaacgctt gtcatgaaaa
       61 tcttgtgctc cacccttagt atttccttct ggatcgtttg tcgcacgcat catgacgtat
      121 actttcttat tagggaaatc acgcatattg aaagatacat gatagccaac atttcctaaa
      181 gtattataag cttgatttac gtctggtcta taaatcccat cagctcttac tctagctagt
      241 tcttttccag tattgtagtc cataatgaaa atatactcgt atttataatt agcaatgtgc
      301 catccagcta catgcaagtt tgcgttttcg atttctccga actgatcaat gtgggcgtaa
      361 tttgttccat ctgtcagcgt agaatttgca gcacctgctc gagttggatc aattactggt
      421 ttatcctccg aagtagttgg attatcatcc gtaaaaccat gagccaaatc ataggctagt
      481 ttttctttac ttacacccat ttcagaaaga taaccgtaag gatctgtatg atcgccccaa
      541 atattttgtg ttacccataa atgcgatttg attcctggtt ggttataagg agtgtctaat
      601 gttaatggaa taccatattt cattgctgaa tctcttgcca attcaacgta tactttatag
      661 ttcttttcaa aagtttcttt atcatgtgtg tgttgtaact cgatttgcac aggacttttg
      721 gctcacgagg ttgtcctact ctacatcttg ctgatagata tgctgaaagg aaaacaccaa
      781 cattatgttt tgctttttca gctagaacga gacaagcagg aataccttta gaagatatca
      841 aagatttttt aggtcataaa gatgtttcca ctacacaagt ctatgcccat atttctcccg
      901 aagtgaaaaa aagatcaatg aatcaactag aaaactatat agaagagcaa ataaaaaagc
      961 actcaaattg agtgcttttt taagctcact atcactaaaa ctatcactaa ttttatttat
     1021 aagcgaacat gtttctatca ctgatttttt aaatctagcg aaaaaccctt atcttaaagg
     1081 cgttcgttca attctttggc aaggtcttca aagcctggct tgcctagaag tgcgaacatg
     1141 gttttcttat agctcatgta gaaaaatttt cttgatgatg attttatcca tcaattgaga
     1201 ttgactgttt tgctctccta tgatttcagc tatgattgtc cgattttgcg gttttcttct
     1261 taactggtag ttgattttcc cagtttccat caactcgcta agcatatcca cgactccgcc
     1321 aaatccggtc tttttcgtaa taaaacctag tgctaccatt cgttcatcta gattttttgc
     1381 tgtaatcgtc acaccagtaa tgagcgagtg ctgtaatgtt tgggccagct cttccatccc
     1441 ttcagcattg gatacgacgg aagctgtttt gttaataggt tcttcgttta tttcttttgg
     1501 ttctttcctc tccactttca ctgtttgacg aaggacatct agttcttgca agagcgtttt
     1561 gtattgttct tgggtcagca tcacgccagc tactttttca cgattaaata tatatacgcc
     1621 agttcccgcg tctcgcgctt gagcaaatac atccatcggc gatcgtttca cttctgtgat
     1681 cgaagatgtg ggaacttcta atttctttaa ttccaatgga tttcctcctt tactaatcgc
     1741 tttttattat agcacagact gacttgtttt gatctttttt ccaaaggttt tatcggtttt
     1801 tgcctaacca gtaattgata tcggtaagaa gtctttcgat accaaatgaa ggatggtaca
     1861 gatacgtttt ttgatcttcg ttatggtcta gtggaaaaaa atgatcggtg atcactagat
     1921 ctgcataccg aggcggctta tttcgagtac atacatgtat agagatccgt tttcgatgtc
     1981 ttagcgtttt gttcagtacc ccagcaagtc ttcttgcttc ttggtgttca tagcgaaaaa
     2041 aaaagacgat tttcacttca ggagggtgga catctaaata ccctcgttcc tggagtgttt
     2101 tcagtaaaag catcccccac tctactgcgg ggtgtgcaag tgcttgttgg atcgctagcc
     2161 gaaattgata tagtttatct ttggaattgg gaaatttttc taatgtttcc tgataataac
     2221 gaacttcttc tatcagccgg tcatggatac caattacgta acgaagccat tcttggtaaa
     2281 gtacttttat cagctttgat ttgctttcgc tctcaaattc gctattcagc caaaacagcg
     2341 aatcaagctt ttcagtcaca atcgtcaatt tccgatagtc ttgtgtctct tctatccgtt
     2401 tccaccgctt ttcagtaatc tggttacagc tcaagcgcaa aattgctaga taattttttt
     2461 cttttattgg caaaatctgc tcctctctaa atagcaattc aggaaacaat tcaaagaata
     2521 gtgttcgact gtctcctaaa atctcgattt ctgccggatc ctttctttgc ttcaataatt
     2581 ggatgaacat ctcagaaaag gactgctctc catcatctgc aaaaaaatgc ggaaaatctt
     2641 tcaaaaagta aaacagcaga tatcttactt ttctttctga accaaagatc tgtgcttttt
     2701 tataagaaaa agaaaggtgg tactcgccaa accactttct caatcgctct tgcgtagaac
     2761 gatcgtactt ctgctcgtaa ggttcattta atagcagatt tttcagtaaa caaaacgggg
     2821 gtgattggat cagaaaaaca aacaagagat aaggttcgct atatccttga tcataaacta
     2881 actgaacggt tttcttatga agaatcagct gaatgggaca gtcgctttcc gccaaacgtt
     2941 tcagatgccg atccactgtt ttaacatcga ttttcagttc ttcagctaaa tcagcttttc
     3001 gccagatcaa atctttcatt agattgatta gtgaaaataa attctgtgca ttttttgata
     3061 atagtttttg ccatctcatc gatcatttct cacctgattt ctaaacatat tgtttgatca
     3121 aaatgagttc actcctaaaa agataaaaaa ctccctttct atttatttaa atacgcaaat
     3181 cttcctcatt attttttatt ttactcaatt taatcgtatt ctcatttcaa aacgaatgta
     3241 ctttaaaaaa acataataag atcactttgc ttgtaggatg tattcccttc cattacaatg
     3301 aaataaatag cgattttatg agaaggtgat gtgaaaatgg aagaaaaaat agaagaatcc
     3361 aaacaaaaaa tcgatcagtt gatataagaa ttagaaaaag cggtcatcgg caaacgttca
     3421 actatccaat tgatgatcgt ggccctatta gcaggtggtc atgtcttatt cgaagatatt
     3481 cccggtgtag gtaaaacgtt actggtcaaa gcactagcaa aagctgtaca gggaaccttc
     3541 agcagagtcc aatgtactcc tgatctgctg cctagtgata ttcttggttt ttccgtatac
     3601 aattcacaat caaaagagtt tgaatttcgt ccgggaccga tctttaccac gatcttgttg
     3661 gcagatgaga tcaatcgtac gactcctcgt actcagtcag cacttttaga agcaatggcc
     3721 gagaatcatg caacgatcga caacaataca taccctctag acaatcattt ttttgtctta
     3781 gctacgcaaa atccaatcga gtacgaagga acatatcctt tgccggaagc acagctcgat
     3841 cgttttttat tccgactaca aatcggttat ccttcttttg atgacgaact tttgctactt
     3901 ttggataaat tcgaaaaagt gttggataac ttaaatgaag tattgaacag ttatgaaata
     3961 gaagaaataa aaacaaatgt ggcgaatgtc tttgtcagtc ccgaagtggc atcttatgcc
     4021 ttgcagttag tgtcagcaac aagaagtcat gctgccatcc agcttggcat cagtccacga
     4081 ggaagccttt cctttattca ggcagcaaaa gcttatgctt taatccatgg gagaaattac
     4141 gtcacgccca aagatttgca ggatttagtt ccttacgtgt tttcccatcg cctgattttt
     4201 tacgatcgtt cattgaagga agacgaaaag aaacaaatca ttcatactct tgttgcacaa
     4261 attcctattc ctgtgaggta gattgaggta gacaatggaa aaaatcaaag attatttcat
     4321 taattttgtg ttatttctgg tatatttcat cattgtgctt tttgctcttg tattcaacaa
     4381 ttctttaggc tggttcttgt tggccttttt tactctttat gtatcatgga gcttgatttc
     4441 attaagcagc tttctaaaga aactttcggt tacttgtaca ttgcagccac tcgtttatcg
     4501 caaaaaaagt atgcaggtta ttttttccat agcaaaaaag agaacttttt ctttacctct
     4561 ccctcgttta caaatcggct tcccagcaat ctttgcagaa aaaaacaaga gattttgatt
     4621 cttacaaata aaccgcaaga agtccaattg ctttggcagc caaaagaaag aggatgccat
     4681 gaaacattaa ctgtcatttt tacagcctat gatagtctag gacttttcag aaaaagtgca
     4741 aaaagagaga tctcttttcc tgtgactgtt ctgcctgctt tttgtaagat tcaagcagaa
     4801 aaattacaga gagttctgga aaaaaaacag cagctgaacg cgtaccaaag aggtcttgat
     4861 tttcgggaac atcgcgctta tcgtccaggt gattctttca accgaatcga ttggaaattg
     4921 tctgctcata ctcaagagtg gttataccgt gaatacgatt atcaagaaga agaatgttct
     4981 cctctttttt gcttttgggg ttcgcccagt ccaaaatttg aacctctgct agacttgtat
     5041 ttttctttat ggcatttgag aaaagaaaag cagccggaac ttctgatatt aggcaaccgc
     5101 gcctttcatg gcaaagaccc cggtcatacc tattttgctt ctttggaagc tggcgatgag
     5161 aaagcttttt tcgcacactt gaaaaaatat gcaaaaaaac aaatcgtcct ctttgttcct
     5221 gagaattctc ctgaagtctc agaagctatt gaaacattgt caaaagatcg aacggcttat
     5281 cttgtttatt tcgtagaaaa acaactcatg gtccaagaaa aagacaagat atgttcattg
     5341 ccaggaggtg aatggattga cgattaaacg ggaaaaatgg ctgcgtggcg tttactcctt
     5401 tgtgatacta agtgtaggtg ctccgatatt tgtagtagct aatcaattgc aaaacagcta
     5461 ccttcttatc ctaactattg gtgtcctatg ccttattgtc tgctgtattc ctaaaaaatg
     5521 ggtacgtttg ctgctttctt tgccagtaat gataggttgt ttgtttttat attttccttc
     5581 gaagacattt tccatattat ggatcttaca gtttttttcg tcagttacag aggaattctt
     5641 ccatctatcc caaggtaaac tttattattt gccagaaaag acagcttttt ttctcataat
     5701 gttgctgatc tatttattta ttacattgac cgtggattac gactattggt acgcaccctt
     5761 tctttctttg atggcttact tcatgatgct taccgttttt cggaaaaaag atttactgta
     5821 cgaaatgatt gcggtagtaa ctgttttgtt cgtgtatctg gcagcaaggc aatttttctc
     5881 catccggttg ttgtcgggaa acactcgatt ccagtcattg actacagttc tgcttatttg
     5941 tttcttaact tttagcgcta tcttgccttt atatcttccg aaagtacacc aatctctcga
     6001 ggaaacgagt gaaccgatcc gagaatatct taatgatgaa gggctttatg acacactcca
     6061 tgagtatcaa ttaaccggaa gtgcaagaac aggcttcagt gagaatgatg aacagcttgg
     6121 tggaccttta tatgataatg gcgaagttgt ttttacagcg aaccaaaaag gaaatggcta
     6181 ttggaagatc gaaaataaaa atcattacac cggttctgga tggatagaag atcaggacgc
     6241 acccatccaa ccaatccaag aattccctgt tgacatcacg agctaccaaa ccgttataga
     6301 agacaaagaa aacattacat tgaccctaaa aaaacaagct ttcttacctt atccttatgg
     6361 aaatactacc ttgactgatt tttcattagc tagtggacaa acgctttact atttgcctga
     6421 aagccagcga ttcattcctg tcccgaatga atcgacacgt tctgcaactt tacaaacaga
     6481 aaggttagtt tttacgagag aagaattaaa aagcagtcag ttaaaagctg atgatttaga
     6541 aaatcagcag acaaatcttc aattgccaaa aagtttgcct aaacgtattg aggacttggc
     6601 ggtcgaattg accaaaaatg aagcaacttt aattcgtaaa gtcgaagctg tctaacagta
     6661 tttaaaaaca agcggaaatt tccgctattc caaaacggat accgggcata ctccgaaaga
     6721 acacgattat gtggattatt tcctctttga ttcaaaagtc ggctattgtg acaacttttc
     6781 aacttctatg gtcgtccttc ttcgaacact tggtatccca gctcgctgga caaaaggttt
     6841 tacaaatggg ctcagaacag gcacagataa tcaagggaat gaggaataca gcattctcaa
     6901 tagtcatgct cattcttggc cagaggtata ctttcctgga tttggctggc ttccatttga
     6961 accaacccct tcatttgcca atccaatggg ttcacaacca gttgctgaaa caaccgaaac
     7021 cgtccagcct gctatcgaac aaccaaatga aagcgaaaat atggcagaaa ctatttccag
     7081 tgagagatct gaggtcattc catcagaaga aacaacactc acaccagctt ataaaatcgt
     7141 cttattcagc cttggagtgt gtttatccgt cttgttgttc tttctattgt tattcagaaa
     7201 atctctgcgc tggacaagtt atcttatcta tcttcatttt tttgcttcta ctaatacgat
     7261 tgcatatcaa aaacttctta accctttgga aacattggta ccgcgcaaac ccgcagaatc
     7321 tttgcaggaa tatagcctgc gaatagaaca agaattcaag ctagatctgc gtttcattcg
     7381 attaactact caatatgaag cagaaatata tggacgacaa gcagatccgg taaaattcga
     7441 taaaaaaacc tatttagaaa taacgcgtat aataagaaaa acggtataca aaaaaattct
     7501 gacaaaaggg agccgcttca aaaaataaga taaaatttct ggagaatcta cttctggaat
     7561 gccgagaacc agctgattga gtatcgatta ctcgaaaaaa aggttgtgac acgttttttg
     7621 tcacaacctt ttttcattat tatttgattt tcaatgtcaa agcatttaga gctacaacta
     7681 ccgtacttaa cgacatcaag acagcaccaa ctgctggact taatatgata ccaattggcg
     7741 caagaattcc ggctgctagc ggaattgcaa gaatattgta acctgccccc caccagaggt
     7801 tctggatcat tttctttcgc gtttgcttgg ctagatcaag aaaatgcaaa atatcttttg
     7861 gatcgctgtt tgtcaatacg acatccgcag aatcgattgc gatatctgtc cctgcaccga
     7921 ttgccatccc gattgaagca cgggctaaac ttggcgcatc attgatccca tcacccacca
     7981 tgattacttt tttcccttgg tcaaggtatt tctggacaac agcttcttta tcatctggta
     8041 ataaaccgcc ataatattct tcgatcccta aataatctgc tactgcttgg gcagcgtttt
     8101 tattgtcacc agtaagcatg acaggagtga tttttctttc tttcaatgtt cggataaatt
     8161 cttttgcttc tgtttttact acgtcaccta aagcaacgat tgcagctaga tgattttcta
     8221 aaatcaggaa gctgatggta tttccttgtt cttggtaagg tttcaataat gcttgttcta
     8281 cgtttaatcc gagacgctca acttcttttt cattaactat tttcacttgt tgttttttga
     8341 ctgttgcctc tagcccgact cctgaaagat tttttagatt ttcagcttga taaggcttga
     8401 tttcatgttc tttcaaataa ttcataatgc caacagccaa tggatgattg gcattcgctt
     8461 ccaaggctcc tagatattga aggatttcat ttttattgaa cgcatcatca agtacttcga
     8521 cacccgttac agtaaatttt ccttctgtta gtgttcctgt tttgtccagc atgatcacat
     8581 ctagatcatt ggcttgctcc agtgcattgc ggtttttcaa aagtaatccg tttttagctg
     8641 ctattgatgt ggagcgagca acaactaatg ggatagctaa acctagagca tgaggacagg
     8701 cgatgataaa gactgttacc atacgttcta atgcattagg aagatctgtc aagaacaacc
     8761 aggcgatgaa tgccaggata cccacaatta atgcgacata aaacagccat tttgccactt
     8821 tgtcagataa tgactctagc ttggattttt ctccttgtgc ttttcgcacc atttccataa
     8881 ccttcgctag ataaccattt tcaccagttc cagttacttc gatttcgatc gttccatccc
     8941 cattgataga accgccaatc actttgtcat tggcttgttt tttgattcct cttgattcgc
     9001 cagtcacagc tgattcgtca acaattgttt cacctttcaa gattacacca tctgttggca
     9061 ttttatcacc agaccggacg atcaaacgat caccttcgtt tacttctttc aatgaaactg
     9121 tctcttcttt tccatctttc gtcaagcgtt tcactgattc tggcagtaat tctgctagtt
     9181 tttgtaaagc attgctggca ttcgacacag cattcatttc aatccagtga cctagtagca
     9241 taataacgat caaagtagcc aattcccaga agaaatccat gacgtgtgta tgtggattga
     9301 gcagatttgc gataaacgaa tagacactat aaatatatgc aactgtaatc cccatcgcga
     9361 tcagtgtcat catagcagga cttttttgtt tcaattccat cttagctcca cttaaaaatg
     9421 gctgaccgcc atagataaat agtatagttg ctaagatcaa tacgagccaa tcagaaccag
     9481 gaaacgttac ttgaaatgga aattccattc ccatcattgg tgaaaaaaga atgattggga
     9541 tagctaggat aagcgatagc caaaattttt gtttgaaatt ccccatgtgc atggaatgat
     9601 ccatgccgac gtgttcgtga tccatgtgca tcgaatggtc catttgtttg ctgtttgcta
     9661 tatctccatg gttatgtccc atgtgcatgg aatgatccat ctgactatgg tcatgtttca
     9721 tctccatctg tttttcatga gattctgtac tttctttgtt tactacttct tttttatgct
     9781 catgtttttt ctccatatcg ttcccattca tttttactcc atcccttctc ttactttttc
     9841 atgtgtcgat ttaagctcag ggagttcaac aatacactga cggaactaaa cgccatcgct
     9901 cctcctgcaa tgattggatt caaaaagcct agtgcggcaa aaggaatacc aatcgtatta
     9961 tagacaaaag cccagaaaag attttgtttg atttttctca atgtcaacct agacaactcg
    10021 attgtttgtg caatggatgc caaactgcta ttcatcagcg tcacatcggc tgtttccatc
    10081 gcaatgtccg ttccacttcc cattgccata ccgacatcag ctaatgctaa ggccggtgca
    10141 tcattgattc catcgcctgc cataccgact tttttgccat ctttctgaag tttttctacg
    10201 taggctgctt tatcctctgg aagtacttca gcaaagatat gctttggatc aataccaact
    10261 tgtttaccaa tcgtttcggc tgccagtttg ttgtctcctg tcagcataaa aacatctaat
    10321 cctttattct ggagctgctt gattgcttgt ttggcttcta gtttgatttg atcagccacg
    10381 gcaatccacc cgatcacttg cttttcatca gctaaaaaca tgactgtttt tccctgctgt
    10441 tctaattcta acgcgtgttc ttggtattca tcaaatgata ggttcaattc aattaatcgt
    10501 ttacgtgtac ccgcaaaata gcgtactcca ttaatcgtac cgctgatccc agcacctggg
    10561 tgggctgtaa aatcaatcat atcgtatgtt gctgcttctt gcagttttcc gtattccacg
    10621 attgctttac ctagtggatg ttcagaagct tgttctaaag tataaaaaat agataggact
    10681 tcttttgaac cgactagatc tgtaactttg ggctttcctt ctgtaatcgt tccagtttta
    10741 tccaaaacaa tgctgtctaa gtgagcagct gcttctaatg cttctccgcc tttaattaaa
    10801 attccatttc gtgcaccaac acccgttcct accataatag ctgttggggt cgctaaacct
    10861 aatgcacatg gacacgcaat gactaataca gagacactat ggatcaatgc gagttgccaa
    10921 tcaccagtga tcaaacctgt agctatgagc gtgataaacg cgatgactaa tacgatcgga
    10981 acaaaaatcc ctgagatccg atctgcgatt ttttggattg gggctttgct cccttgagca
    11041 tcttccacca tttgaatgat ttgcgctaat actgtctgct tgccgacttg tgtgacttcc
    11101 atgtgtagca gaccattcgt attgattgtc ccgccaaaaa gcgtgtcatc cggattcttt
    11161 tctacaggaa gattttcacc agtaagcata ctttcatcta tcgcactgct tcctgaaata
    11221 attcgaccat ctgctggaat ctgctcacca ggacggatga ctagttgatc accaacgact
    11281 acttcttcaa tcgctaacgt ttcttctttc ccgtttctta tcacttgggc ggtttttgtt
    11341 tgaagagaca tcaattgttt gattgcatta cctgttttcg tttttgctgt atgttccaaa
    11401 tatttgccga gcaaaatcag cgtgatgatc atactgctgc tttcaaaata aagatcttgg
    11461 gggtgtccac ggaaaaatcc gttgtaaatg ctcaatgcaa aggcagccga tgttccaatt
    11521 gctaccaata catccatatt tggtgctttc gtcttgattg catggtacgc acctttataa
    11581 aaacgagcac cgacataaaa ttggacagga gcagacagca ctaactggac gattggaaaa
    11641 tgaaaaaagt gaacaatcgc cgcatggctt cctaacatca tagcaatcat tgacaacatc
    11701 aacggcaaag tcaaaaccgt actcaatata agatcgaata gcattttttt caaatacgct
    11761 tgcttttctt ctgcaatttt ttgtttgtgg gcttcatcgt acaaaattgc accataaccg
    11821 atgttttcta cgctttggat taatttttca gtggtcgtcc cttcatattt gacacttgct
    11881 ttttcagtgg ccaagttgac tgtggcattg accacaccag gttgtccgtt cagctctttt
    11941 tcgatcctag cagaacaatt tgcacaagtc atacctgtaa tgacgaacgt atccattttg
    12001 gtttctgctg ttgccatcaa atcacctctg ctttataacc caatccattg attgcttgac
    12061 aaatcttttc gaattgaact tttgtttcat caaacttgac cgtaccgttt gcttttttca
    12121 aatttacttt cactttttgc acgccgtcaa gtgcgcttac tgtttcttct actcttgctg
    12181 cacaatgatt gcagctcata ccttcgattg aaaattgttg tttcattttt tcttcctcct
    12241 attgatgttg tttacattca cattgacctg ggatacagtt acattcgatc gtatctactg
    12301 gttgcttttg ttccagttgt ttcaaaatct cattgatatc ttcttgagtc aaagtggctt
    12361 cttctactag atccgcaatc gtctctccaa tcctttttgc acagatatgg gaaaaaagat
    12421 tttccgtcgc actgcgaact gtttccatct ccgagacagc tggatgataa atgaattttt
    12481 ttccttcttg ttctgtccag atcacttctt tttttaccaa acgtcccagc aaagttttga
    12541 tcgttgctgg tttccaatcc atcgaatcag ctaagatctg cgtgatttct tgggctgtcg
    12601 tctgtccaag tgtccaaata acacgcatga tctcccactc tgaatcactg atctttactg
    12661 gcgttttgac tttttccatt tatttcacac ctttcgttta caaatgtaat cattagctta
    12721 tgaatacaga ttacgcttgt aaacaaaaac tgtcaattat ttttgctctc tattttcaat
    12781 ttagttaaaa attcttgttt ctctcgtctt gttttaagga tgatcagttt atttcttgca
    12841 ttagctactt ggatcagctg ctgtattttc ggttcattgt tttcttcaaa atgccagata
    12901 aaagataaaa attctaagta ttccctgttt agtttctctg aaaatgattc aggcatatct
    12961 ggccgagatt ttttgtttat ctttcttttt agagaacgcc aaactactcg gtagatggcg
    13021 agataacgcg gcactttaag ccaaatgatc ttatctgctt ctttaatcct ttcctctaat
    13081 gtagaagtat aatttccttc aacaatccat gacggattag ataccataaa cgattgctgt
    13141 tcttgcaaaa accacttttt tgcctgaata ctgtagtctg ttgtatgcca taaaaggtct
    13201 agcggcagta gcggaaagtc ggtgatttct ttgatcttcc ttgcgagggt cgattttccc
    13261 gacccacttt gtccaatcac gatgatcttc attcttctcc ctcacttctc tttggaattt
    13321 ggttcgatca taccacttat aacgccgcaa aaaaacaagg gactgtgaca aaagtcgtgt
    13381 caaagtccct tgtttcgaat aaatggtgtt caatcagctg accttcagca ttaactcaaa
    13441 aaacggcaaa atatgaggag ctattttttc gtttgttcct ctaatactca ggtcaaatcg
    13501 ctgattttac aacctcttcc ttaaacggtg ttctcctgcc aacacctcgg tcacaaggca
    13561 caatagtcaa caaacatgag aagttgtttt ttgaaattgc tccttgtgac tcggagttaa
    13621 acggcaggct cactacctct ttcttaaacg gtgttcaaaa gcttgcatct gcggtaataa
    13681 gcccagcaaa acgaaaaata tgaggggcta tttttgtttg gctgttctta ttacttgatg
    13741 caggacagct ttttcacaac ctctgattca cggtgttcca gaagtagctt ctgcgaaaaa
    13801 caagccgaaa cttcacaaaa atttgagaag caattttcgt aaattccttc ttatttcttg
    13861 aagctgacca cttctgtcac aacctcttgt aacaagtatt tctattttaa ggctgaacca
    13921 ctgtttctgg ttcagtgatt tctttgtttt tgtctcgacg agcaattgct ggcaagatca
    13981 tacctaataa gatcaagaca attggtgtga tgatattcgt gaataattgg aaccaccatg
    14041 ctttaggatc agcagtataa tctacttttg gcaccattcc taggatacat gcaaacgcag
    14101 tgaaggcaaa gcaccaagca ccaaagataa aaccgatttt tggattttta acaaatttat
    14161 attcagaatt gaatttctta taagctttat tcaacatcat atatgccaag aacacccata
    14221 ggtaacgcat tggcatgaca actgagttca agttcgtcaa ccattttact aattcattca
    14281 tatctttgat accaaagatc ggcaacaaaa taatgattcc tactagtata cctgtcagaa
    14341 gataaccatt caccaatgta cctttacgat tacgtttacg tagccagttt ggtacaaatt
    14401 ctggatccgc atctgctaac aagattttta gtggtgcatc aattgaaaag gctaatgcgg
    14461 agatttgtcc aatcatgttt gtcaatgcgt aaatgatcat caatgagtta ccgataccat
    14521 agtaattacc taaaatttga aaagcactgt atgccccatt tgccatcaag tctttaggaa
    14581 tattatcgct agcaaacagc atacccattg cgactgagcc taatacagca caaatcccta
    14641 ccattgctgc taagaagatc atccctttag gaaattcctt tgcaggattt cttgtagagt
    14701 tgacataagg ggatattttt tccgctcctc caactgcaaa tactagcatc gaaacggttg
    14761 tgaaataact gaaatcaaat gttggaatat atgttttcac ttgattcata tgtggtgtgg
    14821 caaattccat cgttggattg atgaacggtg ccgttacagc taaaagaata aataagatcg
    14881 acatgacgaa catcgctgtt ccggccagtc cgccaatcac tttcaaagta gaaagtcctt
    14941 ttgttgataa atataaaaag gcaagaaata taataagtga aataactgct accatttgta
    15001 ctgacatcgt atttacgata tttccatttc cttggactgc ccaaccgcca gcaatcagga
    15061 ttgcttgagg cttttgtgcc aaataaggaa tatggacgac ccagtaagtc caagcggcat
    15121 agtatgccag tcgtttatta gatgtcgtat tttcgaccca tgaactgacg cctcctttac
    15181 tatccttgaa ggctgaccct aattgtccaa cgatcaatgc gtatggaata aaataaatgg
    15241 ccaaaatcaa tagccaagag gtgacaactg atactccttg ctgagcatag ttattaacta
    15301 cattccccat gccccaaacc atgttaaatg caattaacgc aacagtaaac cagcgtaatt
    15361 gtttgttgtt cattcgttct ctcctttatg ataaaaattt gttatccaga tgtaatcata
    15421 ttcaattttt aacaaactta caagacagtg ttattttatg tgtgtattct tttatttaat
    15481 acacatcgtt ttttcatatt ttttccatca aaaaaataag ttattttctt ttatttcatt
    15541 taccttattg aatttcaagc tattttgaat tttctgcaaa attaaaaaca ttagttattt
    15601 tataatccta tctattttct ttacattttt cattaagaaa aggatataaa aaaaagagga
    15661 cgttcattcg tcctcttcat tgatcaacgt ttatttttta catgcaagct tcgcatggca
    15721 ttcaaaacag ctaatactgt caccccaaca tctgcaaata ctgcatctcc cattgatgca
    15781 aaaccaaaag cacctaaggc aagtaccaaa atttttatcc caatagcaaa gacaatgttc
    15841 tgtttgacga ttttcaatgt tttctttgct aaatggattg cttctgcaat cttcgatggt
    15901 tgatcattca tgatgactac atctgctgct tcaattgcag catcgcttcc caatccaccc
    15961 attgcgattc ctacatctgc tcttgctagc accggtgcat cgttgattcc atctccgaca
    16021 aatgcggtct ttttattgtt tctttgtaag atttcctcta accgctgcac tttatcttct
    16081 ggaagcaatt cgctatatat cttgtccacg cctacttgct tcccgatatg atcagcaatt
    16141 tttttcgaat cgcccgtcaa catgactgtg ttcttcacac cagccttttt taattgaacc
    16201 aatgcgtcta cggcatcttc tttcaaggta tctgcaataa caagataacc actgtaactt
    16261 tgatcgatcg ctagataaag caatgtaccg atttcttgac ttgaaggaaa agagatttca
    16321 aatctttcca tcaatttgtg attacctgct aatacttgtt tgccatcaac ttcagctaag
    16381 atcccttcac ctgcaaattc ttggatatta gctgttggta aaagcggacc tgcgtagcct
    16441 tctaaaacag attgagcaat cggatgtgta gaaaactgtt ctgcactcgc aacatattgc
    16501 atgaatatct ttggatcgat ttttgtatcg atcgtctgga tcgaaaagtc acctttcgtt
    16561 aatgtgccgg ttttatcgaa taccactgtc tcagtttgtg caagcaactc caagtaattt
    16621 gaacctttta ctaatacacc gattttactt gctcctccga taccgccaaa gaaactaagg
    16681 ggtacggaga tcactaatgc acatggacat gagatcacta aaaatgtcaa tgctcgatag
    16741 atccattcag aaaacgccgc tccagaaaca acaagtggag gtaaaacagc taataaaacg
    16801 gccaatccta caacgactgg tgtgtaatag cgcgcgaatt tagtaatgaa attttcagct
    16861 ggtgcttttt tactgctggc attttctact aattctaata atttgctgat agtagaattt
    16921 tcataagatt tttctacttg aactaaaaat ggtttatttt tattgataaa tccacttaat
    16981 actgtttcac caacatatac agatttaggg actgattctc ctgtaagtgc tgacgtatcg
    17041 actaatgatt ctccttcgat gatcgttcca tctaacggta cacgttcacc aggcttcaca
    17101 agtacaaact gaccgatctg cacgtcttca gggttcacgg tcatcaatcc ttccgtcgtc
    17161 tgcacgtgag ctgtttctgg acggatcgcc atcaattcgc gaatcgattt tcttgaatga
    17221 ctgactgcca atccttggaa atactcgccg atttgataaa atagcatcac tgctacggct
    17281 tctggatatt caccgatcaa catagcgcca atcgtagcaa cagacataag aaaattctca
    17341 tcaaagattt ttcctttgac gatattcatc actgccttct tcactacatc atatcctatc
    17401 agcagataaa tcaataaata ccctatcaag cgaattggtt cattaaaatc aaataatagc
    17461 aataaagcga tacctgcaag ggaaaataaa atccggaaca gcgtatttct ctctttttca
    17521 tttccatgtt cagatacttt ttccttatct atttctgtta ctttgacatc tggttctaat
    17581 tccttcacta ctttttttat ttcttgttca atcgtagcca aatgatcttc ctctgcgtca
    17641 atcgttagtt ttccagaagt gaagtttacc gttgcttcct tgactccatt gatcttctga
    17701 actccttttt cgattttcat tgcacagttc gcacaatcta aaccttctaa tctgtaactt
    17761 ttcattttaa tgacctcctc gcctcaacat atgaatatct cttcataact aattataaat
    17821 gaacggttat tcatatgtca aggtatccga aagaaaaaat gaaaaacatc tgcatcggaa
    17881 aatttcttcc atacagatgt tttggcttat tctttctttt tcttttcgat cattgccact
    17941 gctcttatcc aacccgcact tgccttcttg atcttgacag gatagcaact aagtttgaag
    18001 cctgttgctg gcaatttgtc taaattcatt aatttttcga taggacaata tcttttttca
    18061 atccctgcaa aatgtccttc ccagatattc gaagctccac taagctttca aaaatatagc
    18121 aagagttctt taatgaaaat tatttcaact ttccataaac agtaatccca tataaataaa
    18181 agacgtaaaa gtcacatggg agaacttata caggaaaaca catagagtca ggtgaaatac
    18241 atgtatccca cctgactcta tgcgttttct aacctttatt attctctttt tatacaattt
    18301 ctgcaccgaa tggcatcaac gccaatcgag caagtttgaa agattgcttg gcaaaaggta
    18361 tccctatgat cgtgatacat agaaggatgc cgctgacgac atgtgcgaga gcaattggta
    18421 ggcctgaaat gatcagccag atgatattaa cgatcaatga tatgccagac tggtgataaa
    18481 ttacttcttt gccaaaaggc gcaaaagaaa gggaggcgat cttaaaacat tgcataccga
    18541 ttgggatacc tatgatcgtg atacaccaaa gtatccccgc tgctaaccag ctaagaccac
    18601 cgattagtcc gccgaatata aaccagatga catttcctaa acaactcatt atatttctcc
    18661 ttctattact gtttttgatt aaaactgggc aaaaaatgat tttattcttc tttttttagc
    18721 ttccagcatc aaatattctg caaatttttt ttggatttct tccgtatttt cttttgttac
    18781 taatgtgtgt tggattagtt ttcctgatgg tcgctgatcc aatcgttctt ctgactgttg
    18841 cacgtccgct tgcgcaaatc cgataatccg tttttctttt gttacagcaa cgagcaatag
    18901 tttattctta tcatttaggt cagctgccgt ttttgctttt acgaatgatt ctggatgatc
    18961 agtaatcagc tgattgattt gaggtaaatc ttcttttact gcccacctta tttccatcat
    19021 ttttcacttt tctttcgctc atttttatct ccactaaaaa aatcgactgc ggcaattgca
    19081 tctatccagc tttgtgcaat gatctcatgt ccaccaatcg ttgggtggac cccatcttct
    19141 cttgtatatt tcgaaaaacc ataagcaatc cctaaagcat tgaatatacc atctaaagga
    19201 accagcaatg cgccatattc cttagcaagt ttacgaacaa tttggatttt agggtctaag
    19261 tctttacgcc agccatgacg gtcgtcggtt ataggaagta caaacggctc catcaagatg
    19321 atgttgcaga tttccgctgt ttgaagttca tttagtaatt ttcgataaac ggattcaaat
    19381 tgctgagcag atttctcagt tccgaatata gcctgatcgc tagtattatg ccaggtatca
    19441 ttgattccga tcaaaatcga tacatagtca ggttgtaaag ctaaacaatc ttcttgccag
    19501 cgtacttgca gatctgcaat tttgtctcct cctatcccac ggttcatgca ttcaaattcc
    19561 atcgttggat attgggcgat tagttttccg gcaatcaaaa gtggataacc tttacctaga
    19621 tcagttgctt ctgctcggtc tcttcctgcg tctgtaatgc tatcaccgat aaataatata
    19681 cgttttttca tttgtcaaaa actccttttt gaataaaacg atgattttct tccttatatt
    19741 atcccataag ccctatcaag acgaaagaga aaatatatgt ataaaaaatg acatcagcct
    19801 agtcccttca aacaattgaa tgaacgctca tcaagtgtct agttttttat gctatttttt
    19861 tcttaacgtg ccggattccc cttgattcct tcgttgtttg gtccagagag atcgatcata
    19921 cccaagcttc cgtcaatcag accactgatg acgccatcaa aacatatatt tataggctca
    19981 tctaagactt ctagtaaatg tccctttact aatatctctt ctagattgct tgggatagat
    20041 tggtcataac tttatctggt ccgatgattt caagggtgaa ttgaacttac atatggatta
    20101 ttgcatgctg aaaatacgga cctactccat cttcgaatgt tactattagt ttgttttcag
    20161 gtgctagatg atgctttatt tcaacagcag aagcatcatc aaattagatt tccatttgaa
    20221 ttaccttctt tgtaaatgtt tgattgattg cgatttcgtt atagaacctt gtgaatcaaa
    20281 tagcaatttc ttttgctttc ttctcttgtc tgctggaaac ttcctttatc ccgtatttaa
    20341 aaaaaagaac tgcgagagtt cgcagttctt ttttagaata aacggtgctc tccggccaac
    20401 tcctcggtca caagtcacaa tagtcatcaa acatgaaaaa ctgttttttg aaattactcc
    20461 ttgtgactcg gagctgaacg gcaggcgcac aacctcttga tataaacggt gttcaatcag
    20521 ctgaccctcg atattagcta aaaaaacggt aaaatatgag aagctatttt tccgttttgt
    20581 ttctctaata ctcaggtcaa ggcactgatt tcacaacctc ttatggataa attcggttca
    20641 acaaacgtgg gaatggaata gcttcacgca catggtcgat accagcaatc caagtgactg
    20701 ttcgttcaag gcctagacca aaaccagagt gcggaacaga accgtattta cgtagatcaa
    20761 gataccatga atattctttt tcatccaaac cgtgattacg gatttgttct agcaagaaat
    20821 catagtctgt tgcccgttcg cttccgccaa taatttcacc gtaaccttct ggtgcgatca
    20881 tatctgcaca gatcaccacg tcttctcttg ttggatgtgg tttcatgtag aatggcttga
    20941 tggcttttgg atagtttagg ataaagacag gctgttcgaa tgaattcgca atgaacgttt
    21001 catgaggtga accaaagtct tcgccccatt cgatgtcatc aaatccattt ttcttcagta
    21061 attcgattgc atcatcgtaa gtaatacgag ggaatggcaa ttttgtataa gtttttaaga
    21121 cttctttgtc tcgtcctagt acatctaatg cgtattcgca atgatctaaa acattttgta
    21181 ctaagaatgc tacatattgt tcctgtactt ctaaactttc ttcttgatga gtgaaagcca
    21241 tttctggttc gatcatccag aattcgatca aatgacgtcg tgtttttgat ttttcagcac
    21301 ggaaagtcgg accgaatgaa aagacttttc cgaaagccat agcagcagct tccatgtata
    21361 attggccact ttgagataaa taagcttttt gatcaaagta atttgtttcg aatagatccg
    21421 tagtgccttc tggtgcagaa ccagtcaaga tcggcggatc gattttgaca aaatgatgtt
    21481 gattaaagaa ttcatacgtt gcacgaatga tttcatttct gatctgcata atcgcatgtt
    21541 gtcttgaaga acgcagccat aaatgacgat gatccatcaa gaagtctgtt ccgtgttctt
    21601 ttggtgtgat cggataatcg tggctctcgc ttaccacttc cacatttgta attccgattt
    21661 cataaccaaa ttttgaacga ctgtcttcac ggatttctcc agttactaac acggatgttt
    21721 cttggtgtag atttttagct gtttggaaaa cttcttccgg cacttcgctt tttacaacta
    21781 caccttggaa aaaggctgtt ccgtctctta attgtaaaaa tgcgattttt ccgcttgatc
    21841 gcttatttgc gacccaagcg ccgattgtta ctgtttctcc cacatggttt ttcgaatcga
    21901 tgatttgaat ttgttccacg tatgactctc cttttatatt ccttgaattt cttcaggatt
    21961 aattttgact tttactctca ataaattgct tgattctgcg taccgcttct tctaacgtag
    22021 cgcgatcagt agcataactc atgcggacat tttctggtgc cccaaagcct tcgccagtca
    22081 caagcgctac ccctgtttct tctaaaagat cttctaccca ttgtgtgaca ttttcatatt
    22141 tacacatgcg tagcgtttct tttacattag gaaaaagata aaaagctcct tgcggctttt
    22201 caagagaaac acccggcagt tctgccacaa gcggatataa cgtattcagg cgttcttcaa
    22261 aggcgttccg catttcttcc actgtatctt gctcgccttt caatgcttca acagctgcat
    22321 attgactgac tgctgtcgga ttactggttg actgggaagc aacagcgatc attccattga
    22381 tgatctcaga attccccacc gcataaccaa tgcgccagcc tgtcatcgca tatgttttag
    22441 acacgccgtt gatgatgatg gtctgatttt tgattgcttc tgaaatcgtt gcaattggag
    22501 taaactcatt gccattataa actaaacgac cataaatatc atcagcaact atcaaaatat
    22561 cattttttac tgcccattca ccgattgcct gcagttcatt cttgctataa atcattccag
    22621 tcggattcga tggagaattg atgattacgg cttttgtttt ttctgaacgt gcttgttcta
    22681 gctgctcaac agtaactttc cagctattgg attcttcccc tttcacaaag acaggaagtc
    22741 cttctgccag ttttacttgc tcaccgtaac tgacccagta aggcacagga atgatgactt
    22801 catcttgcgg gtcaaggatc gtttggaaaa gggtatacaa agcaaatttc gctccgtctg
    22861 ttacgactgt ttgtgaaggt tcatagacaa gtccatagtt tttcttcaga taatcaacga
    22921 ttgcctgtcg aagctctggg attcctgctg ttggtgtata atagctagct ttcccattgc
    22981 ggattgcttc gattgctgct tcttggatgt tttctggtgt cgcaaaatcc ggttctccca
    23041 cagtcaaact cagcacatct ctgccttttg cttttaatgc cttggccttt gccgcagctg
    23101 ccaaagtgac agacggttct aatcgttttg ttctttttga taactccatc ccattcattc
    23161 ctttaccttt taaattccct tgattgtctt gacttcatct ccagattcaa agccaatcag
    23221 gtaataattc agtttcccat ttgtttcttt tcctactact tcccacacag gctgatcatc
    23281 atacatccct aaagcagctt tttcgatttg gatttcagga tgggccgcag caattgtctg
    23341 ttttgcttcg ttttcagtca gtccctttgt ttgatccaat acttttactt tttcaccaga
    23401 tttaggaatg ataacggcga tttcttttcc tttatcattt gtacctgtca gtgaaaagta
    23461 tgtcttcttt cgagtaaacc aataaaaatg atcggcttcc tcaagatttg catatttctt
    23521 tgcgatttct actgcttctt tcttcgcctg caccgccgga cgactggaac gcaagtaaaa
    23581 aatcgagctg atcacgatga ccgctaataa aaatacgatc aagccaagta acacgcgatt
    23641 aatcgttttt tcctttgtct gttttttctt caagggtcgt cgctccttta tcttcattcc
    23701 ttttcattat agcagatttt aattatgaaa aaagacagct cctctaactt ccttcttcat
    23761 ttttctttaa gaattcatgg atgtcctgat agatttcctc catagctagc tctttgatcg
    23821 gtactttttt cggtaatgca tggatgatcc gtgcaccata ttttgcagtg actagtcttc
    23881 gatccaaaat aagcaatacg ccacgatctg tctccgtcct tatcaagcga cctaatgctt
    23941 ggcgtaactt caaagctgct ttaggtacgg attcctggta aaaaggattg atcccctctg
    24001 attctaaata agcgttccta gctttgacca tcgggcgaag cggattatcg aagggtaaac
    24061 gtgtgactac aacgatctgc aaagcatctc caggcaaatc tacaccttcc caaaagctgt
    24121 cagcgccaaa caatacgctt ttttctgaca aaatgaagcg tttcaaaagc ttctcccggc
    24181 ttccgccaaa cccttgagct agtatttctc gtcctttttc tagcatagac agatgcattt
    24241 tctgatagac cttctgcaaa acatcatgag aagtgaataa cacaagtatt ggccgatctt
    24301 cttgttcgat cagacctgta agtgctgtct ctaaataatc gatatatgct tgattgctga
    24361 cttgatggac ggcaggtgcc tcttttggca catagatcct tgcctgtttc tcataatcat
    24421 aaggagaggc aatcacttta agaggtgctg cttcaatgcc aagccgctta gagagatagt
    24481 gtcgattagg gccgatcttc agcgttcctc caatataaag aatacgatca tatcgaggat
    24541 accaaacagt cgacggcaac atagccgctt gaaaatcatg gagatgaaaa atcatctgct
    24601 gcgctgtgtt cccaggaacg aaccaatgga tcaaatgatc tcgccagtct tccagccatt
    24661 gatccatcag caagtactgc ttgtctaatt gatcaaataa gctatataaa cttgctaaat
    24721 cgatccgcat ctggtttgtc cagctttctt ggataatctc aaaagtcaga cgcatttgtg
    24781 actgtagctg ctggatctcc tgataaaaaa gctgcaggtg atcaatggct gtcccagatt
    24841 ccagtggcag ctgatccaac atctcttttg tcatcatgac ttcttctgga tactgtccgt
    24901 taggttgctt gccgctcact aaactcatta atccttctgc catacaaatc tgtgcttcgc
    24961 atagcgcaga aagttcttct cgataaatcg ataaccagcg taaggcttca tgttctttcg
    25021 gtaaatagct ttgtattcgg tcaaacagct gatttggttc ttctaattgc tggatttgtt
    25081 tatggaaata gttaatactt aatcgtttgt catttacttt ttcagcgatt tccggcaaat
    25141 gatgtacttc atcgatcaac aaaaatggac tagctggaag caatggttct tttcgctgtg
    25201 tttcttgtat taaaaacgca tgattgacaa tcaaaaaatt actttgcctg attttctttt
    25261 gcaagaaacg aacaaagtct tgttcgtaaa atgactgatt tcttgctaaa aatccaatac
    25321 ctctgtgcgc gatatctgca aagaatgaat ggttcaaatt tgttaaatgt aattcatcca
    25381 aatcaccggt cttcgtttgc gtcagccaaa ctaagaccgc catttgatac aaagcatatt
    25441 gtttttgttc tggcggttcc acaagtgtgc ctttaaaacg ctgtaaatca atatagtggc
    25501 gatagctttt gataattaca ggttgtatgg gctgatctag caattgattc agcaatggaa
    25561 tgtctttttc tatgatctgc tgctgcaaaa ctagcgaaac ggtactgatg acagcgggtt
    25621 tttcaggtgt agccaaataa ctcaatggca gcagataacc gattgtttta cccattcctg
    25681 tagctgcttc gatcatcaaa tcttttgatt catcttttgt gaaatggtcg tagaccgcat
    25741 tcatcagtct gttctgttct tttcgataca tcagtgtttt accaaacatt ttttcttttg
    25801 cttgtttttt tcttggatat gtccgctcac cataatggac ggatgtgaat agttccactt
    25861 cttttttctg caaagctagc ccatcgacga tttctaatga gggatcaagt ggctctggtt
    25921 tttctttcat ttcttcaatc acatgataaa tgaaacgact cgtatccata cctgtgtgtc
    25981 tacttaatcg cgcaatctgc tctaacgtga tgattggcaa ttttctcatc cgttcttcga
    26041 tcagcaaaag aagttgacct gtcacttcgg catcactatc tgcctgatga ggattctcat
    26101 gaataaatcc aaggctttct gacaaatcag ctaagcgaaa gcttggttcc gtcggcaaga
    26161 aaatttgtgc aagttctact gtatcgatgc ctggtatctt caaactagga agtccgcatc
    26221 gcatgagttc atggttcaaa aaattataat caaaataaat attatgcgcg acaaatatgg
    26281 tgtctgccaa aagattgtaa atcgtttggg cgacatcttc gaaataaggc gctttttgga
    26341 cgcgttgatt cgtgatccct gttaaatgct ggatttgttt tgaaatcttc cgccctggat
    26401 gaatatcgat cgaaaaacgt gaagtgatcc gacctgattc aatcatgacg cagccaaatt
    26461 ggatgatccg atcttctttt ggattcgtcc cagtcgtttc gatatcaacg actgcataag
    26521 ttcttggttt caaaatatct tcctcttctc ctgcctctaa tgttcttttg aaattatact
    26581 acaaagtgca gaaaaatact ctcatcgctt tttaaagaaa
//
LOCUS       DAZUKC010000037        25137 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000037 DAZUKC010000000
VERSION     DAZUKC010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.038"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            67..1707
                     /locus_tag="ACG80T_001461"
     CDS             67..1707
                     /locus_tag="ACG80T_001461"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFS4841115.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            1861..3183
                     /locus_tag="ACG80T_001462"
     CDS             1861..3183
                     /locus_tag="ACG80T_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFS4841116.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3245..3676)
                     /locus_tag="ACG80T_001463"
     CDS             complement(3245..3676)
                     /locus_tag="ACG80T_001463"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4841117.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3740..4576)
                     /locus_tag="ACG80T_001464"
     CDS             complement(3740..4576)
                     /locus_tag="ACG80T_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFS4841118.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4766..5068)
                     /locus_tag="ACG80T_001465"
     CDS             complement(4766..5068)
                     /locus_tag="ACG80T_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFS4841119.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5094..5525)
                     /gene="ruvX"
                     /locus_tag="ACG80T_001466"
     CDS             complement(5094..5525)
                     /gene="ruvX"
                     /locus_tag="ACG80T_001466"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFS4841120.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5522..5788)
                     /locus_tag="ACG80T_001467"
     CDS             complement(5522..5788)
                     /locus_tag="ACG80T_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFS4841121.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6042..6767)
                     /locus_tag="ACG80T_001468"
     CDS             complement(6042..6767)
                     /locus_tag="ACG80T_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFS4841122.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7133..7203)
                     /locus_tag="ACG80T_001469"
     tRNA            complement(7133..7203)
                     /locus_tag="ACG80T_001469"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7169..7171),aa:Gly,seq:ccc)
     gene            7320..8072
                     /locus_tag="ACG80T_001470"
     CDS             7320..8072
                     /locus_tag="ACG80T_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFS4841123.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8088..8567
                     /locus_tag="ACG80T_001471"
     CDS             8088..8567
                     /locus_tag="ACG80T_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFS4841124.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8818..10398)
                     /locus_tag="ACG80T_001472"
     CDS             complement(8818..10398)
                     /locus_tag="ACG80T_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFS4841125.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10391..11134)
                     /locus_tag="ACG80T_001473"
     CDS             complement(10391..11134)
                     /locus_tag="ACG80T_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4841126.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11310..12086)
                     /locus_tag="ACG80T_001474"
     CDS             complement(11310..12086)
                     /locus_tag="ACG80T_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFS4841127.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(12334..13170)
                     /locus_tag="ACG80T_001475"
     CDS             complement(12334..13170)
                     /locus_tag="ACG80T_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFS4841128.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(13181..14110)
                     /locus_tag="ACG80T_001476"
     CDS             complement(13181..14110)
                     /locus_tag="ACG80T_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFS4841129.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            14302..14655
                     /locus_tag="ACG80T_001477"
     CDS             14302..14655
                     /locus_tag="ACG80T_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFS4841130.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(14729..15385)
                     /locus_tag="ACG80T_001478"
     CDS             complement(14729..15385)
                     /locus_tag="ACG80T_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFS4841131.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            15611..17545
                     /locus_tag="ACG80T_001479"
     CDS             15611..17545
                     /locus_tag="ACG80T_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS4841132.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(17631..18854)
                     /locus_tag="ACG80T_001480"
     CDS             complement(17631..18854)
                     /locus_tag="ACG80T_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS4841133.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(18848..20719)
                     /locus_tag="ACG80T_001481"
     CDS             complement(18848..20719)
                     /locus_tag="ACG80T_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFS4841134.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(20740..21858)
                     /locus_tag="ACG80T_001482"
     CDS             complement(20740..21858)
                     /locus_tag="ACG80T_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS4841135.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(21941..22774)
                     /locus_tag="ACG80T_001483"
     CDS             complement(21941..22774)
                     /locus_tag="ACG80T_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4841136.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(22767..23684)
                     /locus_tag="ACG80T_001484"
     CDS             complement(22767..23684)
                     /locus_tag="ACG80T_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4841137.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(23911..25035)
                     /locus_tag="ACG80T_001485"
     CDS             complement(23911..25035)
                     /locus_tag="ACG80T_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS4841138.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT         7752 a         5017 c         4289 g         8079 t
ORIGIN      
        1 ctttttgact atatcctcat ctgactccta tacttcgttt gtattaaaac ttaggaggaa
       61 tcattcatgc ttacagggct aaacttatta aataatcctt ttctaaataa aggaacagct
      121 tttacaaaag aagaacgggc aaaatacgga atcactggta tgcttccaag tactgtccaa
      181 acattggaac aacaatctgt ccaagcttac ggtcaatact taagcaaaca gaccgatttg
      241 gaaaaacgta tcttcttaat gaatctattc aatacgaacc ggacattgtt ttataaattg
      301 atgggcgaac atttagttga attcatgcct gtcgtttatg atcctgtcgt tgcggattca
      361 atcgaacaat acaacgaaat tttcttgaaa ccacaagatg ctgtatttct ttcaatcgac
      421 gaaccagaaa atatcaaagc aagtttgaaa aacgcagcag atggacgcga cattcgtttg
      481 atcgttgtga cagatgcaga aggaatctta ggtatgggtg actggggtgt gaacggtgtt
      541 gacatcgcta tcggtaaatt gatggtctac actgccgcgg ctggtatcaa tcctgcacaa
      601 gtcttgcctg tatcaatcga cgctggtact aataacgaag aattattgaa taatccatta
      661 tacttaggga atcgtcatgc tcgtgtagaa ggcgaaactt actacgaatt cattgatcaa
      721 ttcgttcaat cagctactga attattccca gaattgttat tacattggga agattttgga
      781 cgaggaaatg ctgcaacgat cttagaaaaa tacgaagata aaatcactac tttcaatgat
      841 gatatccaag gcacaggaat cgttgtacta gctggtgtat taggtggatt gaatatatca
      901 ggtgagcaat tgaaagatca aacgatcttg acatttggtg ctggtactgc tggtgtaggt
      961 atcgcaaaca tccttttaga tgaaatgatc cgtcaaggtg tacctgaaga agaagcacgc
     1021 caacatttct atcaagtaga taaacagggt ttattgtttg aagatacaaa aggattgact
     1081 cctggtcaaa tcccattcgc tcgtaaacgc tcagaattca ctaatgctga tgaattgaca
     1141 aacttagaag cagttgtaaa agcaatccat ccaacgatca tgatcggtac ttctacccaa
     1201 cctggagcat tcactgaaga aatcgtgaaa gaaatggcgg ctcatacccc acgtccaatc
     1261 atcatgcctt tgtctaatcc aacaaaattg gcagaagcaa aagcaaaaga tttgattgaa
     1321 tggacggacg gaaaagcttt agttggaaca ggtatccctg ctgctgatgt ggaatataac
     1381 ggtgtaactt accaaatcgg tcaagcaaat aatgcgttga tgtacccagg tttagggctt
     1441 ggtttgattg cttcaacagc tacacgagta aatgctgaaa tcatttctca agctagtcga
     1501 gcattaggcg gaatcgttga tgtaacaaaa ccaggagcag ctatcttgcc tccagtagca
     1561 aaaatcactg aattctctca aacgattgct gaaactgtcg caaaatcagt tgttgcacaa
     1621 aaattgaatc gtgaagaaat cacggatatc aaagaagctg ttgaatctgc taaatgggtt
     1681 cctgaatata aatctttaga agattaagat tgttatagta aaagaacata cagaaacttt
     1741 gtatgacgtc taaaaagaaa aaagcgactg cggcaaaaca gcagccagcc aactactgct
     1801 cttttgccgc ttctctttgg cttttttcat acgatacaga ataagaaagg atctgtaaaa
     1861 atggcacaaa aaacaacaac cgcaactgca gttgaggaaa agaaaaaaga agggttttgg
     1921 tcaacaaaaa ttaccggcgt aagtttgccc ctttatttga tcatggtcct tgtcctgatc
     1981 attgcaatgg ctttaggaaa attaccgacg aatatgattg gcccaatcgc tgttttggtc
     2041 attttcggta atttatttca ttttatcgga acaaaaatcc ctatagtcaa aagttattta
     2101 ggtggtggct ccgtttttgc gatctttgcc tctgccgcga ttgctacatt tgggatctta
     2161 cctgagtatg tagtaaaatc cacagaaaac tttgtcagca atatgggctt tttagacttt
     2221 tatattgctg ccttgattac tggtagcatc cttggtatga accgtaatct attgatgaaa
     2281 gcttctgttc gttttattcc cgttgcactt atttcaatgg tcgtctcctt ttttgccgta
     2341 ggactcgtag gtatgcttat cggaaatggt tttgcaaatt ccgttttata tatctcattg
     2401 cccgcaatgg ctggtggtgt tggagccgga gctgtacctt tatcaacgat ttatgcaaat
     2461 gttttaggta cggacgcttc ttctatcatt tcccgcctga ttcctgcgac agcaatgaca
     2521 aatgtcctag ctatcattgg tgcagcgtta gttgctcgtg cgggacaatc aatgccaaaa
     2581 ttcaacggta atggtaaatt aatggaaaaa ggcgaccttg gcgatggaaa acctagaaat
     2641 gtaaaacctt ccattccaca attaggtgtc ggtttgatgg tatcattgac tttcttcatc
     2701 ttaggacaaa tctgtaacta ttttgttcct aaagtccatg cttacgcatt catgatcatt
     2761 atcgttgtta tctgtaaaat cgcaaaccta ttaccagaat attacgaaga tgcagctatc
     2821 atgttcaaca acttgattgt caaaaacttg acagcagctg ttcttgccgg tatcggtatt
     2881 gcgttattga atctaaatgt attggcttct gctttgacat ggcaatttgt tgtcttatgt
     2941 ttgacaagtg ttatcgcgat ttcagtagtt tcaggatttg tcggtcgttt gtttggttta
     3001 tatccaatcg aatcaactat tactgctggc ttatgcaaca actcgatggg tggtacagga
     3061 aatgtagctg tcttatctgc agctaatcgg atggaactta ttgcttttgc tcaaatgggc
     3121 aatcggcttg gtggtgccat cgttttgatc atctcaggtt tcttgatgca attactttct
     3181 tagtcaaaaa aaatacctga tcatcaagta tcggcttgat gaccaggtat tttttgataa
     3241 aaatttacag gattttcaaa aatctttttt gcattttatc ggaatgataa ccgatgcgtt
     3301 cataaaatcg gtgtgcttct actctgtatt ctgcagaatt aagtcttatt gctgatattc
     3361 ctcgtctttt cgcttcttct acgccagtca tcaaggcctt tccgattcct cttttttcag
     3421 catgtttaga cacagcaagt gccataatat tgaacatcgg atcgctgaaa atcgattcat
     3481 aggactccgc ttgtacatag ccagctgctt ttttagaagt aggatcttca tagaccaaga
     3541 aaaaattttt ttctggctcc ttcaacagtt tcttcatttg tctcaccgta acggttaatg
     3601 gtacatcata tcctaattgt tccgcattga tttctcgtag tgcttctgca tctcctaaac
     3661 gcatttcccg caacatccaa atcaccagcc aatttttatt tttttaaatg aattttttta
     3721 tcacagtata ctcctttttt cacttaaaaa aagacttttc agtgattttg acttgcttga
     3781 atccagatcc ttctaatgtt tgcaagatga cgaaagcatt gtaaagattg atcaaaagaa
     3841 ccacgatgac aatcgcttga aaaataaagg tcactttttt tggagacaaa accctgctaa
     3901 atcttgctcc ccacaaacct ccaataacag cagcggggat aacataaaac aacatagata
     3961 aatcaaagcg cataaagcta cttgttaaag caatcgttac caattttgcc agttgagaaa
     4021 agaaaatcgt agatagagaa tatagtgttg cttcttttat tggcaatgca aacatcagca
     4081 tcaacaaaga aacattgact ggtccaccac caatacctaa aaaactagct aaaaagccca
     4141 gaaccccccc acatataagg taccaagtcc atgacgtcaa atgaaatttt ggctgatgat
     4201 gcttggtgta gaagaacgca aaaaccaagg tcaataccgt caaaaaaatt tggatcagct
     4261 gcacatgctt ctcgttttca aataactgta aaaaaacttc gaatacaatg ttgccgacta
     4321 ctcctccaac aacagctcca ctagataccc aaccgacaat ctgccaattc agtgatttcc
     4381 cgcttgatac ttgtcgcaca gtcgaaacaa tggacatcgt gaataccgca actgttgagt
     4441 aaaaagaaat ccctgcaaca gaatgggcgc cgataagatc aagaatcggt ttgatcaaca
     4501 cgcctccacc catcccggaa atagcgccaa ctgtgttcgc cagaacgatc accacaaaat
     4561 aaatcagacc aatcatcttg tatttccttt ctcttgttta tatcttttag cgattgttac
     4621 tcttttcttt tattatacag taaaagaaac aaacgaggct tcgaaagctt ttctactttt
     4681 taaggaaatt tagtgctaaa cgaaaaaacc gggctctact tttcagagag tccagctttt
     4741 cttacacctt atcgattctt ttgttctatt cttcttccgc catgaatgta ttgaatactt
     4801 cttcgatcat atcccattct gcgtctgttt cgatttgttc taactcgcct tctgttccat
     4861 cttcgttttc gatgtatgca taagcttgta attctacatc ttcatcttct gaaacaccag
     4921 ctggatataa aagaacatag ttacgtccaa attcttcttg tccgtcaact gttagcaaga
     4981 tttcgtataa tgtttcattt ccttggtcat ccaccaatgt gatgtgttcg tgtccttcgt
     5041 gttcgtggga atggttatgt tctgtcataa gattacctct tttcttttta ttattaaaat
     5101 aattgcttac ctgagccgtc taaataattt tgtaaaatca tcacagctgc taatttatcg
     5161 atgacttttt ttcgtttcgc tcttgaagta tttgcctgct ctactagcat acgttctgcc
     5221 tgaacagtgg ttagtcgttc atcttgataa tcaaccggca aggagaatag ttctttgatc
     5281 ttctctccat atgacatgga agcttctgcc cttggtccga tggaattgtt catattctta
     5341 ggtaatccaa ctacaaaacg gtctacttcg tactgcgcca ctaattcctt caaccgatca
     5401 aaaccaaact cttcttctgc ctcattgatt cgaatgattt ctactccttg agctgtccag
     5461 ccaaagggat cgctcacagc aacgcctact gtcttagaac cgacatccaa gcccattact
     5521 ctcataaatt cactccatga tttgagaggt agtctttgac aattacttcc atgatttcat
     5581 cgcgttcgtg acggcgaatc aaatttcgtg catcacgata acgaggaatg taggccgggt
     5641 ctccagaaag caagtagccg acaatctgat tgattggatt gtagcctttt tcttccaaag
     5701 cccgataaac aatcgctaat gtttcgctca cttctttttt gcgactatcg tcaaaaccaa
     5761 aacgtacggt ttcatctgta aaacccatga ttctacacct ctttcccgca aggtaattcc
     5821 agcttacctt ctacaccctt attttactcg aagaaaggga aaactacaaa caaatattcc
     5881 aaacttctga ataaattaag aaacacgatt tgtctgattt ttcctttttt ctgtggcttt
     5941 ttcaaaagcc ctctttccat aaagtatacg ctatatttct ttgaaagtat gtttttacac
     6001 aattttttca taaagttcat aaaaaattcg aattgctgct actaattttt cgttttcgtt
     6061 gcttgccctg tctcataatt gatggtgtag ccatcttgaa cattgtagat aaagacattg
     6121 aattcaattt ttttgtcttc aatgctttct gcttccaact gcacacctct tgctaccagt
     6181 tcatttccct cgaaatgagg agtgacacga tagcgtacat gatgatttgt ttccttgata
     6241 tatgccgcaa tatcattttc gtgggcaagc atgtacggag cattcaacga acgtgtccct
     6301 gtcattaagt ttttaatgtt attattttga ccagtcaact ggaaaccaat caaatggctg
     6361 cggttgtata accattgtcc gtcacttaat ttcttttgtt tccaacctgt cggatcaata
     6421 tacaaaggtt ctctcttttc agtaggaaac aagtcttttc ccaacatcgc attggcttgt
     6481 cctactctgt tcaatttgtc tatatttgaa tagctttccc atgtcccttt ggaaagacta
     6541 agatcctctt ttgtaaactc cggctgattc ccattgattt ctatttcttg atgttttcca
     6601 tcatattctg gcacatccgc tacttgagaa gcttgcggtg tggaatcaaa aatggttgta
     6661 atttgttgga agatatctga atctttttgt gtggtggaaa taccgagtgc ccctgcaatc
     6721 acagcagcga cgatccccac gatcgccttg gttgtttttt tgctcatttc tttactcctt
     6781 tactaacagt tccctaaatc ttaacatatg ttcccctata aataaatact ggaaagaaat
     6841 agaaacagta aaaaaataaa accagttttt agacggctta agaatacaaa aaaagagacg
     6901 gattcatcgt ctctactcaa tcacaaaaca ctatttacaa ggtatttatt atcattcaaa
     6961 aaatttttct taatactcaa aactaaacgg cttctgtacg acctcttggt aaacagtgtt
     7021 caaaagcttg catctgcggt aataagtcca accaagtcaa aaacataaga agcttttttg
     7081 cttgactatt cttattactt gatgcagggc agctttttca cagcctcttt taagcgggtg
     7141 acgggaatcg aacccgcgac atcagcttgg gaagctggga ttttaccact aaactacacc
     7201 cgcgatacaa aaacagtata cgcctctttc actccttcct caagtttttt tattctttac
     7261 gcttccgata tgttttgtag taaaataaaa ctaatcaatc aattattgga gaagatgcta
     7321 tgacgggaaa agaattagat ctgttgctgg acacttgttt actcgctggg aaaatcatga
     7381 tggaaagcaa tgcggagatg tatcgggtag aagatacgat gagtcgaatt gctttagctt
     7441 ctggaaattt ccgtttggtc agctatgtca ctcaaaccgg tctctttatt ggtcttgatc
     7501 gtacctcgac catccgaatg gaacaaatta ccaaccgttc gattaattta gaaaaagtag
     7561 taaaagtaaa tgatctttca cgtaaatatg ttgcttcaga gctaacatta gaagaacttt
     7621 actgcgaact gcaagcaatc gaccgagacc gttctttctt tcctgtctgg ttgcaaatcg
     7681 ttagcgcggc agtgatcagt ggaacaatta tggtgttatt tggcggaaag atgaccgatt
     7741 tacctgtgac tttagtcatt ggaggattgg gatacgcagc ttatctatat agtttgaaat
     7801 tttttagaat caaattttta tcggaatttt tatcttcttt attgattggc atagccgcta
     7861 tctacagcgt acgttttggc ttaggaagca atcaggatct aatcattatc ggatgtgtga
     7921 tgcctttagt tcctggagtt caaattacga acgctatccg tgatttgcta gctggacatt
     7981 atgtctcagg agtttctcgc ggaacagaag cgatgatgac tgctacgatg atcggttttg
     8041 cgattgcttt tattttccag cttttttatt aggaaaggaa cacaagtatg gggacgatat
     8101 ttattcagtt ttcttttagt tttttagcga ctgcggcatt tgccgttatt acaaatgtac
     8161 ctaggcgttc attgatttgt tgtggtcttt ctggtgcttt tggctggatg atctactgga
     8221 tctgtgttca aataggcgga tctgctgctt ttggttcatt gcttggttct ttaggagtag
     8281 cattcatcag cgatcttttc tctaaacgtt tgaaaatgcc cgtaacgatt ttcaatattc
     8341 ccgggatggt tcctctagtc cctggtggtt tagcttatca agcggttcga aatctcgtta
     8401 ctgaatccta tcgagaagcg gctcattatg cggtccaagc aattatgatt gctggagcaa
     8461 tcgctttagg tcttgtatta tcagaagtct ttaaccataa catccgtaat ttcaaagtaa
     8521 agcgtgacgc aatcatgatg aaacgaaaaa aacggctttt taaataaacg aagtgtaaca
     8581 aagaaaaggt tgtgaaagag acgttcagcc ttgagcgatg acaaaatatc tcacaaacat
     8641 tgcgacaagt aaccgcagga gcaataagaa gtaattatga gtattaaaaa aagttttgta
     8701 aaaatagctt ctcacatttt tacataaaat tgacttaata ctgagaaact gacttttgaa
     8761 caccctctat ctgtaaaaaa ggggctgcga catgattttt gtcacggccc ctcttcttta
     8821 ttcaaatttt ttccaaaacg ttcgttgata ataaacgatc catagagcgg aaagtattag
     8881 aactaaaccg atcacaggaa tattcactaa ccaaaatgga taaaacaaag ccaacattcc
     8941 gtataagacg ataagaataa tactgatgat caatgaaccc atcatagttg cgactaaacc
     9001 caaacttcct gaacctctgt taaataattg gttaatattg gtccagttca gcagcagcaa
     9061 ccgataatct ctagcgaaat aacgtagtga caataagtag cagccgaata aagaaccaaa
     9121 tagcaagcta gggataaaaa acaaaggcag ctgaaaagac agtccgctta ataatgcgat
     9181 cccaccagtt aaaaaactct gcaatatcca tccgaatctg aacttttctt ttaaatattg
     9241 gttcatagag attggcaaag aacggataaa cagaaaattt tcttgatcca aagaaataag
     9301 attgctgata aaagaagtct gattaactgt taagaaagcc aatgcgatcc ctaccaggaa
     9361 aacaacaccg attaacttat tatctaaatc agttaatttt atttcgcctc ctagggcaaa
     9421 agcaagaata aaaatgattg gagttaacaa ggaagaagaa agaacttgca taatcaaatt
     9481 aggttctttt actaattgag cattgtaact aaataaaagc tgatgcacat tttggttctt
     9541 tttatgggtt ctttttattt tgaactcact gtttgaatca gtcagttgct ctgtaagctt
     9601 tggtactatc agctgtttta cagcaaggaa aaatcccaga gccgtgatag ctagtattcc
     9661 ccaacccaat aaaccttgtt gagaaagtgg ctgactggca gcatcataga atggcaataa
     9721 tatcgaaatc ggatgccgat ctgttaactc attttcaata gtccccgttt gatgattcat
     9781 ccacatgatt ccagcaaatg cgatcagcat cgagctgcca agcaagatac tagaaactgt
     9841 ttttctatgt tttttaaaca ctgctgttcg agccaaacca aaaacaatca aactagccag
     9901 acaaaaaaga ataatcaaaa atagagaaaa caaaaggatt gagataatga tagtaagcaa
     9961 aatccctaga tcagctcgcc atccggttaa aataaacatg actagaagcg gaaaaacaaa
    10021 aggaatgatg gtcaaagcaa ctaccaaaat ctttgctgaa aaaacctgtt cttgtccaaa
    10081 aggcagaggc aaaaatactt gcaggtcatt tccttcaaaa aagatattat aaatgactga
    10141 tatactttga gttaaaccta acataccaaa aagtgccacg taataagtaa agaaacctgg
    10201 caatttaaca aagttcatag caaacatagc aaatccgtaa atcagtaaaa aaatcagccc
    10261 tgaaaaaaga ttttgagaca aaagatagat agttaatcgc tttccctttt ttcctttctc
    10321 tcttgcttta ttcgtgagct gtggattaac atataaaaga ttaactcgaa ttaattctaa
    10381 taattgtcgc ttatacatac ggtccatctc ctgtcgcttg ccttccagcc attttcaaat
    10441 aaatcttttc tagcgtttca tcaggtgagc cattcaataa ttcactaact gccccctgat
    10501 aaatcaattc acctttcttc aaaatagcca gtttatcgca taactgttgt gcggtatcca
    10561 atacatgtgt agaaaaaatg actgtttttc cttttgcggc atgagccttc atcatttctt
    10621 tcaaatcaaa tgctgcttga ggatcaagtc cttgcaaagg ttcatccaat acccaaatat
    10681 ctggatctgg cagcaacgtg ccgatcaaaa tcgttttttg acgcattcca tgtgaaaaac
    10741 ttgcgattgt ttcattttga tgactataca tatcaaaaag cgctgtcaat tctgctaaac
    10801 gcttttcttt tttttgtgtg tctaattcat aggcagctga aatcaagtcc caatattctc
    10861 cagccgtcaa ttgtaaaaaa atatctgggg aatcaggcac ataaccgatt ttttgtttga
    10921 tactcaatcg attttccgtc aatttcattc catctactaa aatcgttccc gatgatggtt
    10981 caataatact aactaaactt ttaatcgtag ttgattttcc ggcaccatta tgtcctaaaa
    11041 atccaaaaat ttccccttgt cttatcgaga gagacaagtc ttttaatgct tgttttttcc
    11101 cgtaactttt acttacattg atgaattcaa tcatttctgt actccttttg tttgttatta
    11161 ctttttattg tacccttcgt tattggtgga gtaaatgtca aaaagagtgc ataacaaaaa
    11221 aggatagaaa cgactaggct gctacttggg ttgttctctt ctacaggaaa acactcttct
    11281 aacaggtaag atatgagtct ttgttttctt taaaatgatt cattttctat cttttcttgc
    11341 ataaaattct ttagtttatc aatacttctc tttcccatct ctagttgttc tccatttgca
    11401 aatgttacta tttcgtttac tctgctaata gatgtaatgt tttcagtatt aatgatataa
    11461 gcttttaaat ctttacataa atactcatat tcgagtgatt ttttatattt tttaattgtg
    11521 ccgtttacga gaatctcttc atctttcaat actaggccta ttttattttt ataacctggc
    11581 atgctcatta gataaaggat ttcatcgtaa tggatatagt gatcgctatt accataacgg
    11641 acagttgtcc accttttttt cgtagcatgt tgaacaggat agttttcttt ttctttcaat
    11701 aagtaacttt ggatgctttt tttatttttt tcataagtga attcttttat caaataacca
    11761 ctaggcgtaa tctttcgatt aataacagtt attgctttat tcggatgaga cgttagataa
    11821 acaattttac acttttcatt cttttctcta agcatttcgc ctagttcgat accgctataa
    11881 taggcgttca aatcgatgtc taggaaaaaa atatctgagg aatagatttc attattttta
    11941 atgaattcaa caaattgtat aacgttcaat ccctgaacga tctcaaattt ccaaggactt
    12001 aataaaattc tggattcttc caaaatttct actagcattt tttcaaaaac caaactatca
    12061 tccaaaataa atatacgcgt taccataaaa agtacctcca ctcgatattt tactatcaaa
    12121 atcatttcat acaacttttc aacatacatg ttaggaataa attgtaattc tcttcacaat
    12181 cttttccaac acctttgaaa ccgatatcaa atactttcag gatgctgctt ctaaaataaa
    12241 tatcaaacaa atgaatacat catgcaaaaa aagccagaca accgacgtcc ttgcgtgtca
    12301 gctgtctgag ctatgtttat ttattttgtt cttttaaaaa gaacgataca catactgatt
    12361 tttcggagca gagactttct cagcgtttgt aacaactaca gatgggattt cacgcttgaa
    12421 tggttgataa tagtctgatt ggatggttcc ttctatttta taccattcat catttttaac
    12481 cgttactcct tccggcatat gcgtcaacaa accaaagact cctgaatctg ccacacaatg
    12541 gataatgccg aatcggaaca agaagatatc tgtattgcta tcgttcggtg tttggtaaac
    12601 aaatccttca tagctgattt ttttgccaat gaattcgcta gggtaattat aaattagctc
    12661 cataacttct aaatagtttt catctgtgat cttgatagtt tgcttatccc catacttttc
    12721 tttcaactca ttcatttggt ctaagtagtc tgatttgttg aaataaatac tggtatccgg
    12781 ctttaagtat tgtgtctgca tctccggatc ccctactgat tctttactta ctgggaaatt
    12841 aaatcctttg gcttctacga tcgtagtgtc gagactaact gttggaaaca acgtccccac
    12901 gattactggc aaagctagca gtacataagc gattccacgt tgataaggtt ttgacaaccc
    12961 atggtcatga tcgtgctcgt gtgccttttc ttctgctttt ccgtttttga cccacaaata
    13021 cagctgaact aaagccaaaa gagcagataa aatcattgaa agaatagcta agtagcgata
    13081 atggacattg atatattgat cgagtttgcc agaaacttgc aagtacatca tcaattcgaa
    13141 atacccagat aaaatcaaaa accgcagcat attttccctc ctaaataaat aatgtgaatc
    13201 caataacgct aagggcaatc agtccgatca gcgataaaat aaagcgtcct gtaaaatatc
    13261 gtttcatcat cagtagattt ttgatatcga ccattgggcc aaagaccaaa aaggcagcta
    13321 caggtgctgt gccgaacaga cttaacaaag aagatcctat aaacgcatct gcttctgagc
    13381 aaagagacat cgtaaaggcc aataacagca tgatgataat ggctaaaatc tttgttgagc
    13441 cgagcgttag cataattcta gttggtaaat aagtctgcat cgcagctgcg attaatccgc
    13501 caatgatcaa ataacgacct gtatcaaaaa attcgtcgat tgaatgtgtc aacacatgcc
    13561 cagtttgatg gatcagctga ttttttggtt cattcttctc actttgttct tcttcatgga
    13621 catgatgatg gtgattttct gctactttag acgtgatttt ttctttcaat acagcgtctt
    13681 tttgaaaata ggcaagccag acaccaatca caagggcaac aaaaaagctg cctatcaccc
    13741 gccagcttgc catctgccaa gaattgccaa atgcgataaa agtggaaaaa atcacgattg
    13801 gattgatgat cggagcagtc agcataaaag caaacgctgt gtgaactggt acgccttttt
    13861 taacaaattg atgaacgatc ggtacgatgc cgcattcaca tgaaggaaag aaaaatccta
    13921 agatcgaacc agtaaaaata gagaagatac gattttcagg aagccatctc ttcacacgtt
    13981 ctggggtcaa aaatacttgt aaggcgcctg agataagaca gcctaataat acgaaaggta
    14041 atgcttcaat aacgatcgat aagaaaatcg tactcatctg caaaaccgaa tttggaataa
    14101 aagaaaacat gagtttcctc ctttttattt ctagttcatt cgctttgcaa atatacgctt
    14161 ttattcaaaa aaaacaagtt gctctgtaaa tttttacaca actcttattt tttaccaaag
    14221 cacttcaatt gactttttct gttattccca ttaaattaag gtctcataca tatcacttct
    14281 atcataaaag ggagaataaa aatgaaacaa accactaact tgactgaagt tcaagacata
    14341 ctgcaacagt catccgttgc cgtcatttat ctttcgatgc ctaattgcag tgtttgtcac
    14401 gctgttcgtc cacgactaga ggaacttctc acacattatg atattccggc acttcatttg
    14461 gatgcatttg aaacaccaga agtagctagt aaatttgaag tgctgactgc gccagttgtc
    14521 ctgatttttc atcaaggaaa agaagtcttt cggcaagcac ggttcatcga ctttaaaaaa
    14581 atccgttatc tactagatga actgacagca gaagatgatt ccctttctta tgaagaaatc
    14641 ttccgaacaa aataatcgtt gtccaaacag aaaaaagcga ttgaacaagt gaaagttttg
    14701 ccttcatttg ttcaatcgct ttttattttt attctgctgt attttcggaa tgcgcatttg
    14761 cgtcagaatg caagaaatag atcaaggttt gcaattcatt cgtcaaatca acattttgga
    14821 tcaatacatc tgacggagca ttcaatcgag cagcggtaaa gttcaagatt cctttgattc
    14881 ctgcttccgt taactgattg actacttctt gtgctctttc agcgggtatc gttaaaattg
    14941 ctacttcgat ttgttggatc ttgatttgtt ctgacatatc ttccattgga taaacgggga
    15001 taccgtctac gatgcgccca acgatttcct cattcacatc aaaagcacaa ctgatacgta
    15061 tgctattact ttgatggaat ttgtatttta gtaaagcact tcccaagttc cctacaccga
    15121 tcagcgcaac atttgtcaat tgatcgtcgt ttaatgtctt tgcaaaaaag tgcataagat
    15181 cttctacatc ataaccatac ccacgttttc ctaattcgcc aaaataagaa aaatctcgac
    15241 ggatcgttgc actatctact tgaacggctt cacttaattc agttgaagac acttttttct
    15301 ttccagcatt atttaaaatc cttaaatagc gataatacaa aggcagtctt ttagctgtcg
    15361 cctttggaat aatatgatct ttcacgaaat gacctccacg tattgtctaa ttacatcttt
    15421 gatttctcca tcatagcagt atttatagga aaaaatcaat tccattgctc agatattgtg
    15481 aaattttatt ctttattcat ttatttatca tttctgcaaa tctcgcagta taatggaatt
    15541 tatggtagac tactaattga aaaatagttt tttatttgag gagaattcac aaagtagaaa
    15601 agaggattcc atgatattat tacaagccaa tcagattgct cgccattttg gtgcagaagt
    15661 cttatttgaa aacatacatt tagaaatcag tacaggcgct cgtgtcgctt tagttggcag
    15721 aaatggcgct ggaaaatcga ctttattaaa gatcattgct ggaattgaag cacctgatga
    15781 aggaaagatc gctaaaaata aaacagcgac tctcggatac ctcgctcagg acacaggact
    15841 tgattccaat gaaacagttt ggaacgagat gctgaaagct tttgaacaag ttagacaaat
    15901 ggaagagcgg atgcgcgagc tagagatttt cattagcgaa ggtacgccag acacacctgc
    15961 ttatagttct tatttaaaag aatacgaccg gctgcaacac gatttttcag atcgaaacgg
    16021 ctatggttat gaaaatgaaa ttcgttccgt attgcatggg tttaaattcg attcttcatt
    16081 ttatgatcag aaaatcgata ctttatcagg cggacagcgc acacgtttag ctttagctcg
    16141 catgcttttg caacaccctg acattttgat tctagacgaa ccaacgaacc atttagatat
    16201 cgagacactt tcctggcttg agaattatct gcaaagctat actggtgcat tattgattgt
    16261 ttcccatgac cgttattttt tagataaagt agtcaatgaa gtctatgaat taagcagaag
    16321 aaaaatgcgc cattacaaag gtaactattc gaaatatcta gatttgaaag cagaacagtt
    16381 agcaagtgaa tggaaggctt atgaaaagca gcaaacagaa atccataaat tagaagactt
    16441 cgttgctcgg aaccttgttc gcgcctctac cacaaaacga gcgcaaagcc gcagaaagac
    16501 attagaaaaa atggaccggc tggatcgtcc tcaaggggat gaaaaacaag caaactttct
    16561 attcagtata gaaaagcctt ctggaaatgt agtcttgcaa atcgaagatg ccgctatcgg
    16621 atatgataat gtcaccttat ctgaaccaat caatctagat atccgtcgtc aagaagcgat
    16681 tgctctagta ggtccgaatg gtatcgggaa atcgacgttg ctgaaatcaa tcattcacca
    16741 gatccctttt atccgtggaa aagaaacgct agggacaaat gtcaccatcg gctattacga
    16801 tcaaggtcaa gctgatctgc atacaaacaa gacgatccta gcagaattgt gggatgagca
    16861 tcctaccact ccagaaaaag agatccgaag tgctttaggt gctttcttat tttctggcga
    16921 agatgtagaa aaaccaatcc ctctgctaag tggcggtgaa aaagcacggg ttgctttagc
    16981 caagttagcg atgaacaaag aaaatttttt gattttggat gaaccaacaa accatttaga
    17041 tattgacaac aaagaagtat tagaaaatgc attgatcgat tatgacggca cactgctttt
    17101 tgtttcccat gaccgatatt tcatcaatcg gatcgcgaca aaagtcgtcg aactctctga
    17161 aaatggcagc aaagtctatc taggagacta tgattattat ttagagaaaa agcaggaaga
    17221 agaagaaatc gccgaattgt tggcacaaga tgagccagaa tctgctcctg ctcccacgaa
    17281 caaatctgat tattaccaaa gcaaagaaca gcaaaagctt atccgtagcc ttcaacgaaa
    17341 aattactcag gttgaagaag aaatggcgcg gatcgatgaa tggatcgatc agttggaaca
    17401 agaaatggtc gcgccagaaa acctggaaga tcacgtaaaa ctcaatgagc tgaaccaaga
    17461 tttagaagct gctagacaag atcaagaaac aaaattgacg gaatgggaag aactaagctt
    17521 ggaattagaa gaatatgaaa attaacgaaa aaaccaaaac agaaaccatt catgctgttt
    17581 tggtttttct ttttttattt tatcaaacaa aaattccata tttacctttt tcaccaataa
    17641 gaagaccaat tactagtcat tctcactaaa gcttctaaat aatagtaatc accccaaata
    17701 caaggtgtat ttaccccatt atttttgggc ttattataaa cagactgtgt taaaaagcca
    17761 tcttcttctt ccgttgtagc atatctttct ttcaattgtc gcataatttt ccaaacagca
    17821 gactggtata agttcttttc tgttttgtct aatagacctg attctattaa ttctaataat
    17881 ccacaaacca tgatcaccat actagatgta tctttttctt ctctgctaga atcatcaaag
    17941 atcaaatccc aataacaaat ggtatcttct ggcaaccgat tcataaaata attggctaat
    18001 attttgctta aatcaaaata tttttgttct ttcaaataac gataacttaa tgcaaaaccg
    18061 taaatccccc atgcttggcc tctagcccaa caggaattat cactataacc ttgatgcgta
    18121 cttccatatc ttggttttcc atctttgaca tccatataaa atgtatgata agttgaacca
    18181 tcttctctta ctaaataatc tgctgcattt tgaatatgag cctgagcagc ttttttatat
    18241 ttttgatttc cagtttcttc actcgcccaa tacaataatg gaagattcat gcaacaatcc
    18301 atgatcattc taccttgttg tgaaggatca ctaggatctc cccaagcttg tataactcct
    18361 gccttctccc aaaaacgctg aagtaatgtt tctgcagcat caattgaagc ttccttcatt
    18421 tttcgatttc ccgttatttt ataatctgct acggttgata aagaatacaa aaaaccaaga
    18481 tcgtgatgat tagtgataat ttgacagtca atcctgtgga taaaatctat atctgctttt
    18541 tcagctgttt gttgaaataa aggatcaccc gtcttctcaa ataacaacca attaattcct
    18601 gtccaaaatc cttctgtcca accaatattg tctcttaatg gataacgtcc ttttatagga
    18661 cactcgcttg ggaattgata ctgactgtta ctgatacgtt tgattaacct ctccgtgtgt
    18721 ctgcgacaac gctcgatctc attattccat tctttttcca ttaattcttt ataacattca
    18781 tttatccatt tttctggatg tattatctct tcaaacatca ctttatcaag catttcccca
    18841 ccagccttta gcataatttt agtctttttt tattgttttt attttggata gttagcccgg
    18901 ttgtcccatc aaaatcaatt ttgggacgat tgttccatac ttctaaacta taatcccgat
    18961 cttgatgagc aaccactcct gttccgagcc aatgggttcc ttttccaaat gtaccctcta
    19021 atccaggaat aaaagtgttt tcccatgtca tcaagtttgt attgggcaaa cccataatag
    19081 ctgtggattt tcgtttaata gtatcgtcac ttaaacagat aatacctgaa aagccagctt
    19141 cactcaccct atagttttcc ttgtcttctt ctatgattat tccttctttc tcaggagaac
    19201 attcaatagc aaaccctgtc tcatatagaa catattctgt atctaattct actttgtgta
    19261 cgcgaatctg ccagccttca aaaggaataa gccaagttct gatttttgcc tgtaaagtag
    19321 gttcccatgt agaggcaagt acctctttct tacatacaat gtctcgtaca ttatttcgaa
    19381 cgatgaactg tccataggtt ttgatgctct cattcttaaa actggtcgtg atatatgtgt
    19441 ccgctcgctg aatccctagc attgaatcca ttgccatttc tgctttcaat tgattcgctc
    19501 gcggtatcga aaaaccaaat tgactagaat aagcaaattt taaatatttt tcattggtca
    19561 atgtatggaa atttggccct aattgtcccg cattcaaaaa aaatgtatgt ccattatcat
    19621 gttgtatgag caaattggct tctgaaagaa gtttagttcc ttcacgaata ggcataggca
    19681 tttctttagc cgtccaatac tcatggtctt caggtagcgc caacaataaa aaaatcttat
    19741 tcagccataa tggagataac gtactattat agggttcagt caatgtcaat tgagggtagg
    19801 tataaccaat attcaggata cctttttcat caaatatttc tttttgcatc caccatctta
    19861 tattccgatt gactaacccc ttgatttcac ctaatttaaa aggcaaaaga tcggcccaca
    19921 acatagctga ccaaaagctt gaaactgcaa aacgataaat catgcttcga ccaaaaggca
    19981 catttgcacc atcttccgca aaaaaatgaa tatagtctct agaaaaatca atagctcggt
    20041 tcttatatat ctggctaatg ggcttttctg gttctagtac gctataaatc aaaccataat
    20101 aatgaaatgc aaaaggatta taataatctt ggcgacctct tgaactgtct cgataccagc
    20161 cattttgaag ataacaattt tctatattat caaatgcttc tttcagtgct gattctttaa
    20221 ctggataacc aaatttttgg ataattgttc cgacaattat cttaaaaaat tgccaattac
    20281 catctgcaca ttcatattca aaaatttgcg agaaccaatg aataatctgg cttttttctc
    20341 ctgtatctaa ttttttccat aataattgtt catgatgata taacgctaat aacattgcgg
    20401 gcatttctac catacgctgg tctctttcat gtatttctcc ccaataatcg ggatgggaag
    20461 gatctgttcc agcttttatt cctgatagaa ttaatttcaa ccatcgttca tctaattggt
    20521 caattgcggg ccccgctccc cataaaaccc gagagaatcc ttccattcct gcaattttat
    20581 ttccatatcc taccccttga tgtatataat caatttgtgc gccacttgct cgataaaacg
    20641 gctggagtgg ttgatttatc tttttaaata agtcaaagta atcctgtctg gtccttaata
    20701 gtggtactgg atgtttcatt gtattttctc ctctttttac taccaaatag ctaattccga
    20761 ctgttctaat cgaagtaagc cttcaataaa aaaataatca gaatagatca gtggcacatt
    20821 ataagtcagt tctctatggt atgcttctgc tccgcctcca atgatcccat cttcatccat
    20881 attccaattt gcatagtgct tttctgtctt ttggagtata ttgatagcag cttcatgata
    20941 catttttgat cgtacagagg aaacatatcc tgctaattcc agaagaccgc atgcagcgca
    21001 tactccagca ctggtatcaa tattcgaatc ttttacagca aaatcaatga gaggaatata
    21061 accagttttt tcgattttct gaataaaatt tactgctatc ttttctgcta tagctagata
    21121 gtacggattt tttgtgtaac gataacttaa cgcgaatcca tataaagccc atgcatttcc
    21181 tctgctccaa caagaattct cattgtatcc ttgcccgcca agtaaccgaa tatatttccc
    21241 tgattctgca tcaaattctg cgatatgccc ggttgatcca tctttcctca ctagatactt
    21301 agctgttgta tctgcatgct gccgagcaat tttagagaac ctcggatcac cagtttccct
    21361 cgaagcccaa tacagtaatg gtaaattcat catactatca ataatcatcc aaccaggttt
    21421 atggtcattc catgctacta aatagttccc tgaaatatta tagcgtccag caagtaccgt
    21481 agctgcatgt aaccctagtt gtcgactatg tttgttttga gactgttgat aatgtgcaat
    21541 agccgatggt aagaataaaa aacctacatc atgatgaacg tctgaaaaat ggtacagggc
    21601 agattctaat aattgttctt gttgtattgc tgcctctttg aacttttctc gttttgtctt
    21661 gtgaaagagc tgccataaaa taccactcca aaaaccattg gtccaccagt cgattcctct
    21721 gtcctttatt acgtctgtat attttccgtt gtaagaaata tatggaattt tattccccat
    21781 tctgtctaat tccgcatcta tcttttcaga agctacattc aaaatttttc ttgaccattc
    21841 cttcgtctgt tcattcatgg atatcccgcc attcttttaa tttttatgaa tgtggagttg
    21901 ctcctagaac gaactagatg gttaacattc gttatttttt ttacttattt gcagctccaa
    21961 tgtcattctc cttaaattta gactgcgccc acacataaat caagaccatt gggatcactg
    22021 aagttgctaa aattagcata attgcgccat aattaattgg ctcacctgta ctagaaaccg
    22081 tcatattttt gataattaag ggaaccgtat atttatcttt atctgaaagc atgactaatg
    22141 gaaatagata ttgattccaa cttcccataa agataagtaa accactagta ataatcgtag
    22201 gtgataaaat cggtacagcg attttcgtaa aaatcaccca ctcattggca ccatcgattc
    22261 tggcagcctc catcagtgct gtaggaaatt gactgctata ttgtcgcatc ataaacactg
    22321 aagttgtact ggcaatcgaa gggatgatca cacctgcata agtattcgat aatcctaaac
    22381 gactcataat gtaaaataaa ggaatcaatg tggtaaaaaa aggcaccatc ctagatagca
    22441 taactgtatt gaacaacagt ttattcccca taaaacgata ctttgctaat gcataacccg
    22501 ccattgtaga actggctgta gctgaaactg tactgattac agcaataaca atcgaattcc
    22561 aaataatcaa gggataatta tatctcgcca tgatggcttg ccagtttttt aatagcatat
    22621 ccccaaactt gaaattgatt ccgccactta aaatttcttg attactttgc gttgaagcta
    22681 caatcatata taaatatggc atcaaaaata ttaaggcacc aaacacaata atgacgtacg
    22741 taaaaattct gctttgtatc ttttgtttag tcatttttat cacctccgaa tttaaattga
    22801 atagccgata atataacaga tacaaacacc atcgtccagg caatagcaga ggcttttcca
    22861 aaatcaacca tttcaaaact tgttttatat aaattgactc ctacgaccaa agctgaattt
    22921 tctggtccat tggaagtccc aaagaatata tttgggatat tgaaattacc caagccattg
    22981 atagtggcta aaactgtggc cattaaaatg attggcttta gcatggggat cgttaataaa
    23041 aagaattgcc gaaatttcga tgctccatct atttcagcac tttcgtatat ctctttagaa
    23101 atcccttgaa gaccagctaa aaataaaata gtgttatatc ctacactaag ccaaatcatc
    23161 gtgatgatca ttgatattcg tatccaaaaa ggatttgtta accactgtac accgttaatt
    23221 cctagcgcat tcaaaaatcc attgaataac ccagtcggat tgaaaaaagt catgaatacg
    23281 gaagctacag ccaccgttga tgtaatggtt ggaatataat agacagttct aaaaaatcct
    23341 tttcctttta actgtgctga attcaataaa acagcaaagt aaatagctaa tagcatcgtt
    23401 attggaattg atcctgccat gataatcaaa acattcgtgt aagatttaat aaacaatgga
    23461 tctcggaaga tagctagata attgtcaaat ccaacaaatt caaatgagta gccaattttc
    23521 ataaaactaa aagcaaacga gataatgatg ggaatcaaaa tcgtcacaat aaagaaaata
    23581 acaaaaggtg tgaccaatcc ataagcactt tttttatgag acgtatctgt tgcttttttc
    23641 ttgctgttct ttattgtctt gcttggtaac gaagattgca tcatcgttaa gtcctcctaa
    23701 tattttttaa tcaccagtta attttacgcc gtacttatga gcaaaatttt ctgcttcttt
    23761 tagatatgaa ttttcaaaat cacctgtttg ccaatagtta tatgcagcgg tagtcaaata
    23821 agacgtagca tctgaataag tggctggtaa aacagtattt gatgtatttt cattgatttt
    23881 attcaatagt tcatagtatt tttgtcctcc ttagtagtca aactttttct cagctgcttc
    23941 tgtttgatag gccaatgagt tagatgtagc cacgcccagc tccagtgcgt tggctaagca
    24001 ttcttcatct gtcagcataa atgtaagaaa ttgttgtgcc acttgtggat ttttggcatc
    24061 tttagggaca taaaaacttg atcctcctga tactgcacct tttcctggat tttcttttga
    24121 aaaaggaatc agtggagcca tgcgccattt gtcagatgca tcaggaaaat tcaaagacat
    24181 attcgtagca taccagccgc cagcaacaaa cattgcagat tcttgaaatg ctttctcagc
    24241 atcttgtgca ccgtagaaat gtacaatatc tgagtcgatc atttctttga ttaactttgc
    24301 agcatgtttc gcttctttag tattcagacg gatatttcct tcttcatcaa gcaaagattg
    24361 attctgctgg gctagcatgt tgataaacaa ttcttggtcc ccagattctg gcaaggcaat
    24421 tccaaaacta ccagtttttt cttttaatgc ttttgccacc tggatatatt cttcccaaga
    24481 actgatttct tcaaaatcta cacctatttc agcaaatttt tttggttgat aataacaaag
    24541 tgctggggta aaatcactag cccaaggaat aatcgtttca ttactcgttt gttgacgcaa
    24601 aatattcaat ttttgatcat aaaactgatc gccatatttg tcataaaatc catattcatc
    24661 agcactataa aaagcatctt gaaatttttc cagcacacct gctgctgaag tgtctgccat
    24721 ggagataata tccggcatat cttcgtgtgt taccaatttt ggggtgatca tttgtactgt
    24781 tgctgttccc gaagctacat tcacaatttt taagtcaata tttgctccag ttttgcggtt
    24841 atagattttt gcagcatttt caacatagtt tgtcagtttg ccgtttgcga catatacttc
    24901 taatgtagtg gcttttttta tcattggatc tgcgatgttt gctgttactt cttcgttagt
    24961 ttttccacat gcacttaagc tgaataatag ggtaatgttt agcaaagcta tcaccaattt
    25021 gtatcttttt ttcatgtcta tcccttcttt cttttcaagt tcttatataa aacgctttca
    25081 cttatgtata ttaacggaat tagtaacaca aatatttata taaaaaaata ttttact
//
LOCUS       DAZUKC010000038        23717 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000038 DAZUKC010000000
VERSION     DAZUKC010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.039"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(53..172)
                     /locus_tag="ACG80T_001486"
     CDS             complement(53..172)
                     /locus_tag="ACG80T_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFS4841139.1"
                     /translation="MVFQKPLNNFRYIVYYCHTKIKKNKQKETVTKVLSQSLI"
     gene            351..2012
                     /locus_tag="ACG80T_001487"
     CDS             351..2012
                     /locus_tag="ACG80T_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4841140.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2082..2429)
                     /locus_tag="ACG80T_001488"
     CDS             complement(2082..2429)
                     /locus_tag="ACG80T_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFS4841141.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2513..3454
                     /gene="opp3b"
                     /locus_tag="ACG80T_001489"
     CDS             2513..3454
                     /gene="opp3b"
                     /locus_tag="ACG80T_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFS4841142.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3455..4498
                     /locus_tag="ACG80T_001490"
     CDS             3455..4498
                     /locus_tag="ACG80T_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4841143.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4504..5574
                     /locus_tag="ACG80T_001491"
     CDS             4504..5574
                     /locus_tag="ACG80T_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4841144.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5564..6517
                     /locus_tag="ACG80T_001492"
     CDS             5564..6517
                     /locus_tag="ACG80T_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4841145.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6529..7539
                     /locus_tag="ACG80T_001493"
     CDS             6529..7539
                     /locus_tag="ACG80T_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFS4841146.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7824..8645)
                     /locus_tag="ACG80T_001494"
     CDS             complement(7824..8645)
                     /locus_tag="ACG80T_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFS4841147.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8742..9020)
                     /locus_tag="ACG80T_001495"
     CDS             complement(8742..9020)
                     /locus_tag="ACG80T_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841148.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9288..11297
                     /gene="metG"
                     /locus_tag="ACG80T_001496"
     CDS             9288..11297
                     /gene="metG"
                     /locus_tag="ACG80T_001496"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFS4841149.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11776..12363)
                     /locus_tag="ACG80T_001497"
     CDS             complement(11776..12363)
                     /locus_tag="ACG80T_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841150.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12538..13860
                     /locus_tag="ACG80T_001498"
     CDS             12538..13860
                     /locus_tag="ACG80T_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS4841151.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            14015..14791
                     /locus_tag="ACG80T_001499"
     CDS             14015..14791
                     /locus_tag="ACG80T_001499"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFS4841152.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14784..15353
                     /gene="rnmV"
                     /locus_tag="ACG80T_001500"
     CDS             14784..15353
                     /gene="rnmV"
                     /locus_tag="ACG80T_001500"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFS4841153.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15346..16230
                     /gene="rsmA"
                     /locus_tag="ACG80T_001501"
     CDS             15346..16230
                     /gene="rsmA"
                     /locus_tag="ACG80T_001501"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFS4841154.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16681..17310
                     /locus_tag="ACG80T_001502"
     CDS             16681..17310
                     /locus_tag="ACG80T_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841155.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17434..18543)
                     /locus_tag="ACG80T_001503"
     CDS             complement(17434..18543)
                     /locus_tag="ACG80T_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4841156.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18752..19174
                     /gene="mgsA"
                     /locus_tag="ACG80T_001504"
     CDS             18752..19174
                     /gene="mgsA"
                     /locus_tag="ACG80T_001504"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFS4841157.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      19361..19459
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            19497..20033
                     /locus_tag="ACG80T_001505"
     CDS             19497..20033
                     /locus_tag="ACG80T_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFS4841158.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20073..21836)
                     /locus_tag="ACG80T_001506"
     CDS             complement(20073..21836)
                     /locus_tag="ACG80T_001506"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4841159.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21839..23554)
                     /locus_tag="ACG80T_001507"
     CDS             complement(21839..23554)
                     /locus_tag="ACG80T_001507"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4841160.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7647 a         4331 c         4810 g         6929 t
ORIGIN      
        1 tttgcataaa tttggcttaa taccgagaaa ctggcttttg aacaccgttt attcagataa
       61 gggactgtga caagactttt gtcacagttt ctttttgttt attctttttt atctttgtat
      121 ggcaataata gacaatataa cgaaaattat ttaatggctt ttggaagacc atggaaaacc
      181 ttgtacaaag ctattttttc tgaaaaattc cttgaaacct cacgaaaaaa tcattatttt
      241 atgaaaaaga caaaaaaaag tgttgatttt ttaataatta ttcattaaac tcttaaacaa
      301 cttcagaaaa tattaattat tctgaaaatt ccaataaagg ggtacgtaaa atgaaaaaga
      361 aatttacatt tggtttggtt gcggtatgcg gccttgtact tgctggatgt tatggcggca
      421 gttcagccac aagcagtagt tcagcaaaaa gcggttcagc agatggagga ggcgtattta
      481 acttagtcgt gcctcaagaa atgcctacag ctgacttatc agttgcaaca gatacaatca
      541 gttttacagc attgaataac gtttatgaag gaatttaccg tcttgatgaa gacagcaaac
      601 cacagccggc aggagctagc gagttggcag aagtcagtga agacggatta acttacaaaa
      661 tcaaattacg agaagacgca aaatggtcaa atggtgatcc tgttacagca gcggattacg
      721 tttatggctg gcaacgaaca gtagacccag ctacagcttc tgaatatgct tacctgtatg
      781 cgccagttga aaatgcagaa gacattacag ctggaaagaa agataaatca gaactaggga
      841 tcaaagcagt tggtgactat gaattagaaa tcaaattgac gaaacaaaca ccatatttcc
      901 aatatctgct tgctttccca tcatttttcc cacaaaaaca atccgtagtg gaagaaaatg
      961 gggatgccta tgcatctgca agtgataaag ctgtttataa tggaccattc acattagaag
     1021 attttgatgg accaggaaca gatacagaat ggacttataa gaaaaatgat cattactggg
     1081 acaaagatac agtaaaactt tctgaaatca aagtcagtgt agtaaaagaa tcttctactg
     1141 cattgaactt attcaaagat ggccaagcag atgacgtgat cttgtctggt gagttagcac
     1201 agcaaaacgc aaacgatcca gcttatactt ctgttaaaga agcgcggaca agctatatcg
     1261 aattcaatca acgtgaaaag aattcaccat tcaacaatgt gaacctaaga aaagctattt
     1321 cttattctat cgatcgtgaa gctttagtaa aacaagtact aggtgacgga tcagttgtct
     1381 ctacaggatt gatcccatca gatatggtaa aaagtccaac aacgaacgaa gactttgcaa
     1441 aagaagctgg aaaattagtt tcttatgaca aagataaagc aaaagaatat tgggaaaaag
     1501 cgaaaaaaga attgggcgtt gattctttag aatttgattt gatggcttct gatgatgatt
     1561 catcgaaaaa agtaatcgaa tatgttcaaa actcaatcca agaaaacttg gatggcgtaa
     1621 cagtcaaacc aacacctgtt ccattctctg tacgtcttga ccgctcaact tcgggtgatt
     1681 ttgacgcagt actaggcgga tgggctgctg actatgcaga cccaagcagc tttactgatt
     1741 tgtttgtcac aggtaattca tacaaccgtg gtcgctggag caatgcagaa tatgacgaag
     1801 cagttaaagc agcttcatca acagacgctg gcgatgaaca agcccgttgg gatgatttcc
     1861 aaaaagcaat caagatcatc tctgatgata tgggtgtcgt tccggtgtac caaaaagcag
     1921 aaggtcacct agtgaatgaa aaaatcaaag gtatcgttca ccatgcggca ggcgcttcat
     1981 gggattacaa atggacatac gttgaagaat aatcgttaat accgtgagca tgagaaaaaa
     2041 cgaggctgcg gcaaatgtcg caacctcgtt ctcatattca cttatcctaa caaagaaaaa
     2101 agcatgaaaa tagcagaaag agcaattagt attccggcaa tgatcagcca aaagctgtac
     2161 ataccgtagc tggcagacga gagcgaggaa aattctggtt ttttctttcg gttccttgtt
     2221 cgtgcaagat ggaccgatcg ctgaaaatga tcgaagaaac ccgaagaaaa gacccataaa
     2281 aaaccgccga taatcagaaa cggcaatgac cataagaata gatcattaga taattgaatg
     2341 agtgtaaagg aatggttgat gagatttttt attaaaataa ccagaatcag tagaaaagct
     2401 actgcgtagg ggatagattt ttttttcatt tgattgctcc tttatttttg gttcattatc
     2461 taataatgta gcgaaacatc tgtcagaagt caaaagctag ggggaaacat tagtgaatag
     2521 ttatatcaaa tatgtattaa aacgtgtctt tttcatgatc atcacgcttt ggttaattgc
     2581 aacaatcacc tttttcttga tgcaattgct gccgggcacg ccatatacga atcaggaacg
     2641 tttgagtcct gaaacaatcg agatgctaaa taaacaagtc ggtttggata aaccagttat
     2701 cgtacaatat gggatttatc tttctaattt gcttcaagga gattttggaa tttccttcca
     2761 atttaaaaac cagccagtcg cacatttgtt agctggaaga atcggaccat ccttgcagtt
     2821 agggttacag gcaattatct ttggaacagt cctagggacg attttaggaa cgatctctgc
     2881 catgaagcaa aatacatggg cagatacatc ttctacactc gtagcgatat taggtcgttc
     2941 cattccaaac tttgtatttg ctgttttatt acaatatatc tttgcaatca aattacaagt
     3001 tttgccaatc gcaaaatggg atggctttgt gtacacaatc ttaccaacaa ttgcgttggc
     3061 catgtcgccg cttgctgact ctgctcggtt tatccgtacg gaaatggtgg aagtactgca
     3121 tagtgattat gtggaattag caaaagcaaa aggattgagc cgttgggaaa tcgcctttaa
     3181 acatggtctt cgaaatagtt tgattccatt gatgacttta ttaggaccgt tagctgtagc
     3241 attgatgacc ggatcactgg tggtagaaaa tatctttgct attcctggta tcggggaaca
     3301 attcgttaag tcaatcacaa caaatgatta tcctacgatc atggctgtaa cgatccttta
     3361 ctcatttatg ctgatttttg ttattttgat cgtggatcta ttgtacggtc tagttgatcc
     3421 aagaattcgt gtttcagaag ggagccgagg ataaatggaa atgataccaa agcgttatga
     3481 aacagttgca gatatccctg cggatgaatt tcttccatta caaaaaaata ccgaagaaga
     3541 acgtgaacag attactgcac cttctttgaa ttttatccaa gattcatggc gccgtttaaa
     3601 gaaaaacaaa gctgccgtta tttcaatggt tttactattg gtcatcattt tgatctctgt
     3661 gatcacaatc tttgtttctc ctcatgatcc aactgcccaa aacgtggctt atatcaattt
     3721 gcctcctcgt attccgggaa tcaatatcga tggattgaat ggaaaagcaa tggttggtgg
     3781 tgaacttgtt gataaatatg ctcaagcaaa cgtcccttca aacgtaaatt tctttttagg
     3841 aacagacggt ttgggacgtg atgtattaag ccgcttattt atgggaatga gaatctcgtt
     3901 gctgatcgca tttattgctg cattactaga tgtaacgatt ggtgtagctt acgggttggt
     3961 ttctggtttg ttaggcggac gtgtcgataa cgtgatgcaa cgttttttgg aaatcttatc
     4021 aggaattcca aacctcgttg tcatgatttt gatgttggtc gtttttgaac caggaatttt
     4081 ttcaatcgtg gctgctatgg ccatcacaaa ctggatacca atggcacgta tcgttcgtgc
     4141 acaaacaatg aaattaaaag atcaagaata tgttttagct ggattgacat taggtgaatc
     4201 aagaatgaaa attgcattca aacatatttt gccgaatatc tctagtgtca ttattattca
     4261 aatgatgttt agtattccat ctgctatttt cttcgaagcc tttttaagct ttatcggttt
     4321 aggtcttacg cctccgtcag cttcattagg aacgatgttg agcgaagggt acaagacatt
     4381 cctgtatctg ccttatctat tatggattcc ggcagtaact ttatcggtta tcatgattgg
     4441 ctttaactta cttgctgatg gattgcgtga tgcctttgat cctaaaatga aagagtgaag
     4501 acgatgacga aaattttaga agtgaaagac ttagaaatct cttttgatac gtatgctgga
     4561 aaggtacgag cgattcgcgg tgtcaatttt catttgaata aaggagaaac attggcgatc
     4621 gttggggaat ctggaagtgg aaaatcagtg acgaccagaa gcatcatgcg cttgctgtca
     4681 agcaacgcca atatagatgc tggtcaaatc ctctttaaag gtaacgatat cgttggaaaa
     4741 tcagaaagag agatgcaaaa aatccgtggt aaagaaatag cgatgatttt ccaagatcca
     4801 atgacctctt tagatccgac gatgcccatt gggaagcaag tcgctgagtc ccttagaaag
     4861 cataataaag tttcaaaaaa agagggacaa aaagctgcat tagaattgct taaattagtt
     4921 ggaatacccg aagctgagaa acgtatcaac agttatccgc atcagttctc aggaggtcag
     4981 cgtcaacgga tcgtgatcgc gattgcactg gtatgttatc cggaaatcct gattgctgat
     5041 gaacctacaa cagcgcttga tgtgactatt caagcacaga ttttggagct gctgaaagaa
     5101 attcagacaa aaatcgatac atcaattatc tttatcactc atgaccttgg tgtagtagcg
     5161 aatgtcgctg atcgtgtcgc tgtcatgtat ggaggaagaa tcgttgaagt tggaacatcg
     5221 gaagaaattt tctacaaccc gcaacatccc tatacttggg gactgcttgg ttcaatgccg
     5281 acattagata gtgcgaacga tcgattgtat gctattcctg gttctccacc ggatttattg
     5341 aacccgccaa aaggagacgc attctatcca agaaatgaat tcgcgatgaa aatcgatgca
     5401 gaacaagaac caccattttt tgaactatca aaaacacaca aggcagcaac ttggctactt
     5461 gctccgcagg cacccaaagt gacaccgcca gaagaaatcc aacgtcgctg ggctatcttt
     5521 aaagagaaac aaaaacatac ttacggtcaa ggaggaattg ctgatgccaa ataaactact
     5581 tgaagttaaa ggattgaaac agtatttcaa tgtaggaaaa aagaatgaag tacatgctgt
     5641 aaatgacatc agctttcaca tttatgaagg agaaacattc ggtttagttg gagaatctgg
     5701 aagcggaaaa tccactactg gccgtacgat cattcgcttg aatgaaccta ctgatggtga
     5761 gatattattc gacggtcaag atataacgaa gatcaaagat aaaaaaggtt tgacgaaatt
     5821 ccggcatgat gttcagatga tctttcaaga tccatacgct tcgttgaatc ctcgtatgaa
     5881 agtcagagat atcattgccg aaggaattga tgttaatgga ctagctaaat catctgaaga
     5941 gcgtgcaaaa aaagtcgatg atctattgag aactgttgga ttgaatccaa gccacggtac
     6001 tcgttatcct catgaatttt caggtgggca acgccagcgt attggtattg cacgagcatt
     6061 ggcagtaaaa ccgcgtttta tcatttgtga tgaaccaatt tctgcactag atgtgtcgat
     6121 ccaagcccaa gtggtcaatt tgttgcaaga tctccaaaaa gaacatcaat tgacctatct
     6181 atttattgca catgatttgt cgatggttaa acatattagt gaccgaatcg gtgtaatgca
     6241 taacggcttg ttattagaga tgggaacaag tgatgaaatc tataatcacg gtgttcatcc
     6301 ttatacagaa agtttgttat ctgctattcc tctaccagat ccagaccatg agagaaaacg
     6361 cagaagaatc aaatatcaac ctgaaccaga tgacggacaa gtacgtcaac ttcgtgaaat
     6421 cgcaccagaa cattttgtct atgcaactga gcaggaagtc ccatattacg caaaaaaact
     6481 aaaacgacaa aaggaatctc ttctggttgc taactaggag ggacatagat gctaaaagct
     6541 tttaaattcc gtatgtatcc gacagaagaa caaaaacagc aattgattcg tacttttgga
     6601 tgtgctcgat ttacctataa ccatttgctg aaaaagcggc agaaaagttg gcaacaaaca
     6661 ggagtagcca atttttcgct cacacccgct acattaaaaa aagaataccc attcttaaaa
     6721 gaagtagaca gtcttgcttt agcaaatgca cagcttaatt tggatcgggc ttttcgcaat
     6781 tatttcaagg gacgtgccag cttcccaaag cttaaaacga aaaaaagtat gtggcaatct
     6841 tatacgacca acaaccaaac ccgtacgatc tatctggaaa atggctactt aaagttacca
     6901 aaacaaaaag aactaatcaa gataaatcag catcggccag ttgaaggatc gattcgctcc
     6961 gctacgattt ctgctcgata taatgaagaa ttttatgtcg cactactttg tgatgtttcg
     7021 ccagtaaaaa aagaatcttt agctaaatgg atcgggatcg cctatcatcc gaagacattg
     7081 atccaaacat ctcgacctct agaagtaact ttaccaaaat ttcaccagac agaagaaaag
     7141 ctgcaacatg cacagcggaa attgaatgtg aaagtccgct ccgctcacca tagaaagatc
     7201 aggttagatc aggcaagcaa ttatcagaaa caaaagagaa aagtaatgga tctttatctg
     7261 aaacaaaaga accagcgaga agattatctt gaacaattat ctgggaaatt ggtcaaacaa
     7321 tatgactact tatttgtaga atcttttcca aaagaagagg cacatgctga tttttctatt
     7381 cacgactggc ataaattaat aacaaaattg cgttataagt cccaatggta taataaaaaa
     7441 tttcttttga tcaataccga cggagcagaa gaaagcaatt ccgtaagaaa gagccaagta
     7501 gtagaaaaaa tgggcagaca ttcggttatc aagggataaa aaatgaatga aactgatagg
     7561 agcgttaggg ttagccttgt taataaaaag tcttaatgac ttttgtttct aggaattttt
     7621 cttttgaaga aaagctcact aattatgaca aaccaataaa acaatagcag aaaaagttcg
     7681 tacgagcttt ttctgctatt gttaattttt tattcagaaa gtttacgaat acatcaaaat
     7741 tctgaaacaa aaaacgtaac tcacaaagag ttgtttttgc gttttttctt gaatgattag
     7801 gtctgaatct tttgctcaca accttattca tggtgccccg gcttgagctg atagattttt
     7861 gaaggacggc cgattcctac aggacgttca ccgatctcat cgaaatattg gagcatagct
     7921 ttcttaaaat tggaatgatc gatcgtttta taatcgatcc ctaagaattt agcaaatact
     7981 tttcgagctt ccgtaatcgt gaaatcttta cctaaaactt gtagaacttg cggctcgtgt
     8041 tccattttat tgactacacg gttgaatgct ttcaagatga tttgactatg gtcaaaagcc
     8101 aaagtatctt tgccagaaga ctctccagtg actaaatcaa gcgagatagc cacttctccg
     8161 ctagataaat ggatcttgtt actatgacgt tctagtgtga accagcgtac ttccttggca
     8221 tcatctccgg cgattagcgg ttcttctcca ataaaggcta agtagctgac tgtgacgacc
     8281 cagcctcgcg gatctcgatt aggcgtactg aatgtatgca gttgctcaat attttctttt
     8341 gaaataacca caccagtttc ttcctttgtt tctcttaaga cgctttctcc agttgactca
     8401 tccttttgga caaagccacc aggcaatgcc catgaatttc gataaggatg cccttttctt
     8461 tggatcaaca aaactttcag ctgatcttct tctttgttat agcacatcag tacgatatcg
     8521 acagtcaaag agggcttgtc atattctgga cgttcctgtt tttgatacca gttaagaaat
     8581 tcttcttcac ttgctttatg ttcataatat aatttttctt cttctttaga agcaaaacgg
     8641 ttcatctgac cacctccatt gacagtaaaa gtacttttta ccttattgta gcagactctg
     8701 aaaaaaaacc aaggatttac ttgaaaaatc taggttatca tttaaatatg aaaaataaat
     8761 ggaagtatga gattgaataa aaagaaaagc agataaaacg gcaatacttt ataccgctct
     8821 tcacgaatgg caaagaagcc acaaataacg atatatacgt caatgataaa acgcaaaatc
     8881 caagctgctg tcataaaact gttcaaatgc ccatggaggt taaaataacc atacatacga
     8941 atataaaaat agctgaatat taaatgtgct aatgaaagaa aagcaatact aagtgtaaaa
     9001 aaaagatgtt tcttctgcat gattgttcca gtcccttact aagatagata gtctttatta
     9061 taccacgtgt gaggggagaa ataaaaagcc gttttttagt agatatgaaa caaaacaaaa
     9121 acccgcattt attcaaccta ttcatccttt taaaggatag attgaacgta gatagaagtc
     9181 gtttatccag tctttttcat tttgaaaaaa agctaacagc atgctttttt tatggtatac
     9241 tagcaaggaa acgaattcgc atcatgcgat aaaggaggaa gagccccgtg gcagaaaagg
     9301 aaacttttta tatcactacg ccaatctatt atccaagcgg caaacttcat atcggcaact
     9361 cgtatactac gattgcatgt gacgcaattg ctcgttataa acggttgatg ggctttgatg
     9421 tgttttatct cactggagtg gatgaacatg gtcaaaaaat cgaaacaaaa gcagaagaac
     9481 taggtgtaaa accgcaagaa tatgtagata aaatggcggc tgatgtcaag aaattatgga
     9541 aaacgcttga tatcagttat gataaattca ttcgcacgac agatgattac cataaagcag
     9601 ctgttcaaaa aatatttgat cgtttattag aacagggaga tatttactta ggtgaatatg
     9661 aaggctggta ctctgtttct gacgaagaat tctttacaga aacacaactt gcagaagtgt
     9721 acaaagatga cgaaggtaac gtgatcggtg gtaaagcgcc aagtggacac gaagtagaat
     9781 tggtaaaaga agaatcatac ttcttccgca tgagcaaata cgcagaccgt ctattggaat
     9841 actatgaaga acatcccgaa ttcatccagc cagaatctcg taaaaacgaa atgatcaaca
     9901 actttatcaa accaggatta gaggatcttg cagtttcacg aacaactttt acttggggag
     9961 taccagtcaa gagtgatccg aaacatgtgg tgtatgtatg gatcgatgca ttatccaatt
    10021 atattactgc actaggatat ggctccgaag atgaaagtct attcgaaaaa tactggcctg
    10081 cagatgttca gatggtcggt aaagaaatcg ttcgctttca tacgatctat tggccaatca
    10141 tgctgatggc ccttgatttg cctctaccta aaaaagtatt tgggcatggc tggctattga
    10201 tgaaagacgg aaaaatgtct aaatcaaaag gaaatgtcgt ttatcctgaa atgcttgtgg
    10261 aacgttatgg tttagatgca ctccgttatt atttgcttcg tgctgttccg tttgggtcag
    10321 atggtgtctt tacgccagaa gattttgttt cccgtgtcaa ttatgatctg gcaaatgatc
    10381 tagggaactt attgaatcga acgattgcta tgatcaacaa atattgtgat ggactcgttc
    10441 ctgactatgc ctcacttgtc actccatttg atagtgaact gtctacgacg gctgctaacg
    10501 ttgtcggacg ttaccatgat gcaatggaaa aaatggaatt caatacagca ttggctgaaa
    10561 tctgggtgct gatttctcgt gcaaacaaat acattgatga aacagaacca tgggtattag
    10621 caaaagacga agaaaagaaa gctgaattga atagtgtcat gatccattta gctgaatctt
    10681 tgcgtatcgc agcgatatta cttcagccaa tcatgacaga aacgccagag aagatcttca
    10741 atcaactagg attggactct gaaacaatga atcttgaagg attacacttt ggcgaatttc
    10801 cttcaggaac aaaagtagtt gcaaaaggta caccaatctt cccacgttta gatatggaag
    10861 aagaagttgc ttttattcaa gaaaaaatgt ctgaaggtac tcaaacaaac gaagatacag
    10921 tcaaatggga tccagaagaa acagaacttg tttcaacgaa agaaaaacaa atcaaatttg
    10981 atgtatttga aaaagtagaa ctaaaagtag cagaagtcat caactgtcaa aaagtagaag
    11041 gggcagataa gttgctgcaa ttccgactag atgctggaga cagccaagac cgtcaaatct
    11101 tgtcaggaat cgctgaattt tatcctgatc caagcgaatt gatcgggaaa aaagtcgtca
    11161 ttgtagcaaa tctgaaacca cgaaagatgc gtggacaaat cagtcaagga atgatccttt
    11221 ctgcagaagc accagatggc tctttacaag taatcgaagc accaaaatcc atgccaaatg
    11281 gctcagaaat cgcatagaaa ttcaaagggg gtatgacact agttgtgtca tacccccttt
    11341 ttccgaaatt tgagaagcaa ttttcgtaaa ttccttctta ttacttgaag ccaatcatat
    11401 aatacagaaa cagagaacta tccaaaataa atcctagaaa aagaagtacg agcttcgctg
    11461 atttttgtta atttagaata atactaatag gaatcgtaaa aacagaaaaa aagcataatg
    11521 ataaagggtc gggcagattt taattgattc atctggtact cttttttatt gtccttacat
    11581 tcataaagtt tgaatgcaat gactttatta catctatatt tatatctaac agtagcgttg
    11641 atgtgttaat tataagttct atcactttat gttcaattag tttgtgctct tatatgtgtg
    11701 attgaaaata tagataaaaa aacaatcatc ggctttgact tcgatgattg ttttctttat
    11761 tttgtaaaaa tcctatcact tgattttatg gtaacctttt atttttgtct tcaaacggct
    11821 tggtaagaga gcagtaagca gtgcaaatgg gatcgaaaag acataccaga aaatcgtacg
    11881 tccgatacca taatgttgat agagctttgt caacatcacg tgtaacaaga tcaaccctaa
    11941 aagcaaggag ccagctataa accaatgatt atcccaatgc atgacttcac taagtgccat
    12001 cactccaaaa ggatagaaat aaaaatagat cccgactaag gtcatccgcc agttctttgt
    12061 aaatgttccg atattcaata aaaagaaaat cgctgaaagc aaaataccga taggcggaaa
    12121 aagcccaacc aaaaaggaat acaacatccc ccacaagatc atcacaggac ctttgatcaa
    12181 tgcaacgatt gcgatgaaca aagtcagcaa tattagtttt tgtgataacg ttgcaacagt
    12241 cattaatcca aataaaatta atgtgaacca atgccaatgg gactgctgtt gttctttacg
    12301 ggaaaaatcg acccggttgg ataaataatc tgctaatttc ggataagaac gcttaaaatc
    12361 cataaactgt tcctccttcg tggaaaaggt aggtttagta tacgaaaaat aaaaaagaaa
    12421 tacatccgtc ttaagaggga gtttttcact gaaagtggag aaaaaagaat ggtaaattaa
    12481 aatattctca gttatactga aaattatacc aaatttcaaa gaagaaagag gatgggaatg
    12541 aaaggcaaac taaaacgaga aatcaatctg tttggcgcat tagctaccgt tatgggaacc
    12601 gtgatcggtg caggcgtctt cttcaaaact gctgctgtta ctgctagtac tcagtctaca
    12661 agtttgacat tgctagcttg gcttttgggt ggtttcttaa cgatctgtgc tggattgaca
    12721 gtggctgaat tagctacagc aatcccggaa actggtggag ctgtaaaata tattgaagct
    12781 gcgtatggaa aattgcccag ctttttatta ggatgggcgc aaagcttgat ttactttcct
    12841 gctaatattg ctgcattatc gattatcttt gcgacacaat tgacgaactt attacaatta
    12901 tctaccgatt atcttttgtt gattgcagtg atcaccgctg tttctgtgac agggttgaat
    12961 ttgttaggga caaaagtcgg tgcttctgta caatcagtga cgttgattgt taaattaata
    13021 ccaattgctg tgattgtcat atggggatta ttgaccccag gacaaggaac tgtccagtta
    13081 tttccaatcg aagctggaaa agatgttact tttgtcgaag gattgagcag tgcgttgtta
    13141 gcaacgttgt ttgcttatga cggctggtta ggtgtcggtg caatggcagg ggaaatgaaa
    13201 cggccggaaa aagatttgcc taaagcaatt attcttggtt tgagttttgt aacagtcgtt
    13261 tatctattga tcaattttgt ttttttgaaa actttgccga ttgatcatct tgccggtaat
    13321 ttgaatgcag cttctgaagc atcagacgtc atttttggag gaatcggtgg aaagctggtc
    13381 actattggga tcttgatctc ggtatacggt gcattgaacg gttatacact aactgggatc
    13441 cgcgtgcctt atgcgatggc tttagaagac gagctacctt tcagtaaaca gctgacaaag
    13501 ctttcaaaga agtttacggt tccttatgtt cctgcggttt tccagttagc agtagcttgt
    13561 attatgatga gccttggttc tttcgacttt ttaacagaca tgttgatttt tgtgatgtgg
    13621 ctattcagtt tactgatttt tatcggtgtc tttattttaa gaaaaaaagc gccggaattg
    13681 ccacgtccat ataaagtacc actttatcca atcgttccga ttattgcgat tctcggtgct
    13741 attttcattt taggtatgac gatgatgacg caaacgaaat tagcattgat cggtataggt
    13801 gttaccttga taggtatccc agtctattat caaaaaaaga aaaaacgctc agaagagtaa
    13861 aatatttcaa caacagacag aaaaagtacg aactaggtcc tctagatctt cgtgcttttt
    13921 ctatttcaat aaaacaagtt cttctatttt gatttttgct acttctgcaa gtagtgctta
    13981 tgtgctaaag taagaatgaa aaggagagat aaacatgata tttgattctc atacccattt
    14041 aaatgctgaa cagttcaatg aagatattcc agaaacgatt cagcgagcac aagaaatggg
    14101 ggtaacgaaa atggcggtcg tgggcttcga tacgccaacg attgaaaagt ctttgcaatt
    14161 aagccatgac tatccaaata tatacagtat tatcggctgg catccgacag aggctggcag
    14221 ttatacgaaa gaaatcgaaa ataaattaca agaacaattg actttaccaa aggttgtagc
    14281 attaggggaa attggcttgg attatcattg gatggaagac ccgaaagaag tccaagataa
    14341 agtatttcgc agacagattg ctatcgcaaa agaaatgaac ctaccaatca gtattcatac
    14401 tagagaagca cttgaagaca cgtatcaaat cctaaaagaa gaagatgttc gagatatcgg
    14461 tggaatcatg catagtttca gtggtgatta tgaatgggca aaacgatttc ttgacttagg
    14521 gatgcatatt tcatttagcg gtgtcgtcac tttcaaaaaa gcattagacg tccaagaggc
    14581 tgcagcaaat gtaccaatcg aacgattact tgtggaaaca gatgcccctt atctggcacc
    14641 agttcctttt agaggaaagc gaaatgaacc aggctatact cgttatacag tagaaaagat
    14701 tgctgaactt cggaatcttc cgacagaaga agtggctgcc caaacatgga caaatgcgca
    14761 ccgattattt ggtctaagcg aacatggata agatgaagat ccaagagatt atagtcgttg
    14821 aaggaaaaga cgatacaaga aggctgcggg aagtagttga tgcagatacg atagaaacaa
    14881 tagggtctgc aatcaatgaa gagatcctga tgcaaattga acatgcccaa gagactcgtg
    14941 gagtgatcgt ttttaccgat cctgattttt caggagaaaa aataagaaaa atcattatgg
    15001 aagctgttcc tcaggcaaaa catgcttttt tgccgcgcac acaagctaaa ccaaagaaaa
    15061 agggaagcct tggtgtcgag catgcaagtg atgctgctat tttagaagca ttgaaaaagg
    15121 tcgtgacgcc agacatttcc caaacggctg tttctgaaat cactagacaa gaccttttag
    15181 actatggttt aatagctggg gcacaagcaa aagaaaaacg agaaaaacta ggtgacgagt
    15241 tgcgcatcgg ttacacgaac ggcaaacaat tggaaaaacg tttgaagatg tttcgtatta
    15301 cgccgaaaga actcgcccaa gcaatgaaaa tagtggagga acaacatgag tgaatatcgt
    15361 gaaattgcga caccctctag aacaaaagag attttattga aacatggttt ttcatttaaa
    15421 aaaagtttag gacaaaattt tctgactgaa ccaaatattt taaggaaaat cgtagaaact
    15481 gcgcaaatag atgaacatac gaacgtgatc gaagttggac caggaatcgg tgcattgacg
    15541 gaacaattag ccaagcatgc aaaacaagtc gtagcatttg agatcgatga tcgtttgatc
    15601 cctgtattgg ctgatacgat gcaaccctat gacaatgtga ctattgtaca tcaagatatt
    15661 ttaaagaccg atttgtcaac agctgttaga gaaacatttc atgaggagct tccgttaaaa
    15721 gtcgtagcga atttacctta ctatattacg acaccgatca tgatgcattt tcttgaatca
    15781 gatttggttg tggatgaact agtcgtcatg atgcaaaaag aagtagctga tcgtatctct
    15841 gctgaaccag gaacgaaagc ttatggttca ttatcgattg ctgttcaata ttatatggaa
    15901 gccagtttag cattcatcgt accaaaaaca gtatttgtcc ctcagccaaa tgtagattca
    15961 gcgattttga aactgacaag aagagatata ccggcagtcg aagtgacaga cgaaaaagca
    16021 tttttccgat tgacaaaagc ggcatttcag caaagaagaa aaacgctttg gaataacttg
    16081 cagcattcgt atggcaaaga tgaccagaca aaagcatggt tagcaaaaag cttggaaaca
    16141 gctggaatcg atccaaaacg tcgtggggag actttgtcat tacaagaatt cgccgcatta
    16201 agcaatgcga tgtcagaaaa tatgcagtaa aaaaagaggt tgtgacagaa gttgtgaaaa
    16261 agctgtcctg catcaagtaa taagaacagc caaacaaaaa tagttcctca tgtttttcgt
    16321 ttgtttgggc ttattaccgc agatgcaagc ttttgaacac cgtttaagaa agaggttgtg
    16381 ggcctgccgt ttaactctaa gtcacaagga acaatttcaa aaaacagctt ctcatgtttt
    16441 tgagttagtt ggtcttatta cttgatgcaa gcttttgacc actattatgc cgaacaagga
    16501 gatgtgacac aaattttgtc acatctccta tttttgtttc ttgatcaaaa aattcttata
    16561 cttgatactc acactcattt tgtatggatt tagaaaagaa tatgtgtctt gacagttatt
    16621 agtaagtgag ttatactaag tcgacattta tgtgtcaaga catattagga gagagaaaaa
    16681 atgagaaaaa attcagttcg aaaactaacg atttctgctt tattgattgg gatgggggtc
    16741 attatcccaa tggtcatgcc taaaatcatg attccaccag cttcctttac tttggcgtct
    16801 catgtgccat tatttattgc gatgtttttt acaccaggag ttgcagtggc tgttgcgcta
    16861 ggtacgacat ttggtttctt tttaacaacg ccggttatca tcgcattgcg agcattgtca
    16921 catcttgtgt ttgcgttgat tggggcttgg tacttgcaaa agcatccgag tatcgtttta
    16981 aaaaatggac agtttacttt tgctaattgg cgtttccaag ggttcaattt tgtgatcggt
    17041 gtgattcatt ctttagcaga aatgctagta gtcagcatct tttactttat tggtaatatg
    17101 cctgaaacat attattctca aggatatttt tatacagtat tcatcttgat ggggattggt
    17161 gggcttatcc acagtttagt tgattataat attgcttatt ttatagcagg aacactcagc
    17221 aaatattttg atatccccgt atttacggca gcaaaaaaag aaaatcggaa agagtcgatt
    17281 caagggtacg cgaagaaaac gacatattag acatgatctc tcaaaaaaaa aactctcatc
    17341 tagtaatgga taataaatcc tctaagatga gagttttttg tcctaaaaag agctgcacca
    17401 acatcttctg gcacagctct aacagggggc aatttatgga agtttgatga cttgttctgt
    17461 ttctttatca aagaagtggc ctttgctgat gttgaaggca aggtcgacta tttcgcctgg
    17521 tttatggaaa tctcttgcat ctacttttga gatgaactca gtagaaccag tttttgtgta
    17581 aagcatcgtt tcagctccaa gtaattcgga aacgacaact tctgatttca ctacggcttc
    17641 aggtgaagct tcaagggcta cttgttcact gtggatgtct tctggacgaa taccgaagat
    17701 aacttctttt ccttcataac ctttttcaac taatactttg ttttttcctt caggaatacg
    17761 aagcttaagc ccatgtccat ctgtgataac accatttttc aatgtcacat taaagaagtt
    17821 catggcagga gatccaataa agccggcaac gaacatattg acgggtgtat cataaacttc
    17881 tttcggtgta ccgatttgtt ggataaatcc gtctttcatg ataacgattc ggtcagccat
    17941 agtcattgct tctgtctgat cgtgagtaac atagatggtc gttgtttcaa gacgctggtg
    18001 aagtttggca atctcagcac gcatggctac acgtaattta gcatctaaat ttgataatgg
    18061 ctcatccatt aagaagactt ttgcatcccg aacgattgcc cgtcccaatg ccacacgttg
    18121 gcgttgtcca ccagataaag cagctggttt tcgtttcaag tattctgtca gacctaagat
    18181 ctctgctgca ttgtccacac gttttttgat gtctgcttta tcatatttac gtaatttcaa
    18241 gccaaaagcc atattatcaa aaacagtcat atgaggatac aatgcgtagt tttggaaaac
    18301 catggcaata tcacggtctt taggtgccac gtcattcatg acttttccgc cgatatttag
    18361 ttctccttca gagatatctt ccagtcccgc gatcattcgc aaagtagttg actttccaca
    18421 accggaagga ccaacaaaaa cgataaattc acggtctttg attgatagat taaaatcagt
    18481 tacagaataa ttttctgcat tgtcatattt tttatatacg tttttcagag ccatttctac
    18541 cataatacgt tccacacttt cttttttagt tttattaact atttaaagta taaatgaaag
    18601 cgttaactaa cttcaatgtc atgttgaaca agaatttttt cttttttttg tcaatgtgct
    18661 agttttaagc tttaataaat atccataaga aataaaaact gatacactgg agtaaataag
    18721 tatgttaaca atatcaaaag gagtgtctct tatgaaaatc gctttgatcg ctcatgaccg
    18781 aaaaaaacct atgatggtga aattagcaac agcgtataaa acaatattgg aaaaacatga
    18841 actttttgct accgggacga ctgggcagag gatcacggaa gcaaccggct tgccgatcca
    18901 tcggtttaaa tccggaccgt taggcgggga tcagcaaatt ggagccatga tttcggagga
    18961 tcagttagat ttggtgattt ttcttcgaga cccgctggct gcacagcccc atgaaccaga
    19021 tgtaacagca cttatccgtt tatctgatgt ctatgagatt ccgttagcta cgaatatcgg
    19081 aacagctgag gtgctactaa gaggactgga agccggcttc ctagattttc gaagaatcgt
    19141 ccatgaaata gacaagcatc cactttcatt ttgatggata agcgtatgga aaaataacgt
    19201 ttaatgttcg cctatatgta gctataaaag ctaaacaaac tgataatatg cgatataata
    19261 cgaacgtatt ggataatata aacaataata ttagtttttc gtatttgcag taaatgtgaa
    19321 aatatgctat agtttctgtg ggaaaagatc ccaaaaataa tacagagtag aaaataaagc
    19381 gttaagtgct aagaggatgg gatgttgcct cttggacgaa ggacgtaatg ttcgcggttt
    19441 tattttcgca ttcgctgcat gatacgatgt agcagctctt aaaggagatg ctaaaaatga
    19501 agaaacaccg gttagacgca cgtatgattg ctattatggg cttattgatt gcattgatgg
    19561 tcactttgtc ccgactggta gcaatcgaga ccccatttat taaaatcagt gtcacattta
    19621 ttccacaagt gatcatgggc attctttttg gtcctttttg gtcagggata ggcgcagttt
    19681 tagctgattt agtaggaatg gccctcttct cgaaatctgc attctttatt ggatttacat
    19741 tgaatgcatt tattgaagga gcaatctacg gattcttttt ctatcgaaaa gagatcactt
    19801 ggaaaaatgc cattctggct acgcttagtg tcacgctgat catcaactta tttttgacac
    19861 cattatggct tgcgttgatg taccatgtgc cattgttcag ctgggtcgtt tgggcgcccc
    19921 gcctgttaaa aacagtaata tggttaccga ttcaatcaat tgccatttat tacgtaggac
    19981 gtagtattcc ttacaaaaaa attttaagaa gtttagcgat ccacgctaaa taaaaaacaa
    20041 gcagtcttcc catcccagaa gactgcttgt ttttattgtg ctgtgaattg tgcttgatag
    20101 agccgtcggt aataaccatc tttttgaagc agctcatcat gggtgcccat ttcgacaata
    20161 gctccctgat ccatcactaa tatctggtcc gcatttttga ttgtggacag gcgatgggca
    20221 atcacaaaac tggtttttcc ttccatcatt gctaagaagg cttgttggat ttttttctct
    20281 gtcaatgtat cgactgagct ggtagcttca tctaagatca acataggggg attactgatc
    20341 atcgttcgag caatcgtcaa cagttggcgt tgtccatctg atatctttaa cccttgaccg
    20401 ccaatcttag tatctaattt ttccggcaaa cgttctacaa attcatacat ataagaggct
    20461 tttagggcat cattgatttc tgcgtcactt gcttccggat tcccaaaacg aagattttct
    20521 cgcaatgtac tgtcaaatag ccaagtatct tgtaacacca ttccaaaact tttacgtaat
    20581 gtatcacgtt ggatagagcg gatgtcatgg tcatcgactg taattgttcc ttttgttgtg
    20641 tcataaaaac gcatcagcaa attaacaagt gtggattttc cggcccctgt ttttcctact
    20701 atagcaatgg tttgacctgg ataagcagta aaattgaaat cttcgatcaa acgctgcttg
    20761 gggtcataag aaaaagaaac atgatcaaaa gagacttttc ctttgatatt tttgggttcc
    20821 aaggcatagg catctggcaa atcaggcttt tcttctggct gatcgatcaa atcaaatgct
    20881 cgttctagac cagaaaaagc ggtttgcagt tgtgtcgtga ttccagaaag ttcgataaat
    20941 ggtttagaaa actgacttga gtagatcgta aagctggaaa tcacaccaac agtgattgac
    21001 gaaccactct tcaatgccaa taggccgcca actaaaccaa tcgataaata agcaaggtga
    21061 tctacaaaac gagaagacgg gtttgtcaag gaagaagaaa attgtgcccg ttgtcctttt
    21121 tcatatagat cagcatttag cagttcaaat tgctgttgac tagcttcttc ttgctgaaaa
    21181 gctttcacga ttttctggtt accgattcgt tcggtcacaa agcccgagat atccccaata
    21241 atcctttgct gtctatcgaa atttgtttgt gaagcacgag caacgatcca actcacaagg
    21301 aaaataattg gtgtagaaca aagaacgacc aatgtcagaa gtggacttag acgcatcata
    21361 aaaataaacg aaagaatgat gattgccact ccagaaaata attgattgaa ggccgcagaa
    21421 attgcaatgg aaatattgtc catatcattt gtaaatcgac tgacgatatt tccgtgggag
    21481 ttttgatcat aataacgaag tggcaatcga ttcagatgag caaatgcatc ttttcttaat
    21541 tttgcaacgg ataagtaagc taaccgattg cttaacactt ggatgagcca ttgactgact
    21601 acgttgatta aaacgatacc accgaataga ataagtaaat ggaacagtcg attgaagcgg
    21661 acctgtcctt ctccaatcat ttgatcgata ctttgaccaa tttgtaacgt catcacaact
    21721 gttgcaatcc cattgatgat tcctagtatc acagctagcc aaatgtcttt tgcataggca
    21781 gtaaggtaag gaatgaatcg gcggagcgtc tgcaaggata atttttttac tttcataact
    21841 attcttcctc ctgtgatgcg acgatttcct gatattctgc tgattgagca agtaattctt
    21901 catgggtacc ctgagcagca atcttacctt gatccatcac taagatatga tgtgcttctt
    21961 gaatagagcg gacacgttgg gagataataa tcactgtact gccaagataa gtctgtagcg
    22021 ccttacgcaa atccaagtct gtttgataat ccaacgcact taaagagtca tctaaaatca
    22081 gtaatgctgg cttggcaatc aaagctcttg caatcgtcag tcgctgtttt tgtccgccag
    22141 agaaattttt cccattttcc atgacttttg tatctaaacc atttggaagc tgacggacaa
    22201 attcttctgc ctgagcgatt ttcagagctt cccaacattc tttttctgtg gcgtctttct
    22261 taccccattg aaggttttca cgaatagtcc cggtaaatag tacagaagtt tggggaacaa
    22321 gagcaatttg ctgacggaga tgatccagct gccaagaacg aacatccatt ccgtccatta
    22381 taactgtacc ggaactgaca tcatagaaac gcggaatcaa ttgtgtcaaa gtcgttttcc
    22441 cgcttccggt tggaccaatg attccaagga ctgtatttcg cttcagctga aatgtaatat
    22501 tttggagaga aagaccagaa tcaggagtat aacggaagtc aacatggtca aaagcgataa
    22561 ttgcttcaga agaaaaatcg ggagtagtac tgacctcagc ttcaagaatc gaattttctg
    22621 tgtctagcac ctctttcacc cgatttcctg aagcttcggc acgtgtgaag atcacgacga
    22681 ggtttgaaac aacgatcagt gccaaaagca tttgattcat ataattgatc aaagcaagaa
    22741 cttgtccttg ttgcaagcta ccaatattta ctttgatccc gccaactgtc agcaaacaga
    22801 tgatcccgac attcatgatc aaggttgtag caggtgataa tagggcagag atattggaaa
    22861 cacgtaaata attgttcgct agatcatttg tgcttttatc gaacttctct gtttcttttt
    22921 ttgtctgcgc aaatgcgcgg atgacacgaa caccgctaag attctgacta acgtgttcat
    22981 tgagctgatc tagtttttct tgcacttttt ggtagagagg aacggttttt ttgatgataa
    23041 aaaataaaat gattgagaaa ataggcaata atgctaagaa aaacagacct acttcccagt
    23101 cgatcacaaa agccatgacc actgacccga tacttaaaaa cggtgctctg atcaataaac
    23161 gaatgaccat tgctaaagct agctgaagct gattgatgtc gtttgtcatt cgggtgatca
    23221 aggtatctgt tccaaaatga ttcaactcgt tgtgagagaa agtattgatt tttttgatta
    23281 gctgattacg taactctgta ccaaatcctt gtgaagctac tgaagcatag tattggcaaa
    23341 tgatagcaca aatcagacca atcacagaca tagcgaccat ccataagccc atttcaatga
    23401 tttttcctcg gtcattcatt tttagtccat tatcgatcaa ggaagccatc attaaaggca
    23461 ggacaagttc gaagacagct tcaagaaatt tgaagaacgg gcccaaaatg atttgcttac
    23521 gatagtcttt tgcataacgt aataactgaa tcatatgatt cctcctatta tttatttgtc
    23581 cacaattgaa aaaacaagta ctaccacatt gtagcttagg aaatttagaa tgaaaagaga
    23641 acaagccttt tcataaatga aagttttatt atgaagaagc tttagaaatt gaatagacta
    23701 ttttttttaa aaaaaag
//
LOCUS       DAZUKC010000039        23418 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000039 DAZUKC010000000
VERSION     DAZUKC010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.040"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..74
                     /locus_tag="ACG80T_001508"
     tRNA            2..74
                     /locus_tag="ACG80T_001508"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="ACG80T_001509"
     tRNA            78..150
                     /locus_tag="ACG80T_001509"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="ACG80T_001510"
     tRNA            155..225
                     /locus_tag="ACG80T_001510"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="ACG80T_001511"
     tRNA            238..311
                     /locus_tag="ACG80T_001511"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="ACG80T_001512"
     tRNA            323..394
                     /locus_tag="ACG80T_001512"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     regulatory      583..662
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            660..1853
                     /gene="metK"
                     /locus_tag="ACG80T_001513"
     CDS             660..1853
                     /gene="metK"
                     /locus_tag="ACG80T_001513"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFS4841161.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="ACG80T_001514"
     CDS             1983..3455
                     /locus_tag="ACG80T_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFS4841162.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="ACG80T_001515"
     CDS             3538..4326
                     /locus_tag="ACG80T_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4841163.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="ACG80T_001516"
     CDS             complement(4393..5061)
                     /locus_tag="ACG80T_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFS4841164.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="ACG80T_001517"
     CDS             5176..6888
                     /locus_tag="ACG80T_001517"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4841165.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..8660
                     /locus_tag="ACG80T_001518"
     CDS             6891..8660
                     /locus_tag="ACG80T_001518"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4841166.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(8865..9431)
                     /locus_tag="ACG80T_001519"
     CDS             complement(8865..9431)
                     /locus_tag="ACG80T_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4841167.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(9431..10387)
                     /locus_tag="ACG80T_001520"
     CDS             complement(9431..10387)
                     /locus_tag="ACG80T_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFS4841168.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(10387..11043)
                     /locus_tag="ACG80T_001521"
     CDS             complement(10387..11043)
                     /locus_tag="ACG80T_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFS4841169.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            11413..13431
                     /locus_tag="ACG80T_001522"
     CDS             11413..13431
                     /locus_tag="ACG80T_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS4841170.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    13501..13707
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13858..16272
                     /gene="leuS"
                     /locus_tag="ACG80T_001523"
     CDS             13858..16272
                     /gene="leuS"
                     /locus_tag="ACG80T_001523"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFS4841171.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            16620..18173
                     /locus_tag="ACG80T_001524"
     CDS             16620..18173
                     /locus_tag="ACG80T_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFS4841172.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(18554..20338)
                     /locus_tag="ACG80T_001525"
     CDS             complement(18554..20338)
                     /locus_tag="ACG80T_001525"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4841173.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNFAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(20353..22074)
                     /locus_tag="ACG80T_001526"
     CDS             complement(20353..22074)
                     /locus_tag="ACG80T_001526"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4841174.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLQIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            22174..22935
                     /locus_tag="ACG80T_001527"
     CDS             22174..22935
                     /locus_tag="ACG80T_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS4841175.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IRERKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         7400 a         4203 c         4866 g         6949 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta aatccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattatttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtagctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaagatg aagtcaaacc agaaaactgt aaagcatttg
     7921 atggtgttga aaaaggtctt gcgttgcagc acgtagattt tggttattct ccagaaaaac
     7981 tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga aatggttgct cttgttggtc
     8041 caacaggatc ggggaaaacg acgatcatga atctgctaaa ccgtttttac gatgtcaatg
     8101 atggttcagt agtcatcgat ggaatagata tccgagatta tgatcttgat gcattacgta
     8161 gacatgtagg aatcgttttg caagaatctg tattgttctc cggaacgatc aaagacaata
     8221 tcacattcgg caaaccggac gcttcagatg aagaagtcat caatgcagca aaacaagcga
     8281 atattcatga cttcatcatg acgttagaaa aaggttatga tacagagata tccgaagaaa
     8341 ataacttatt cagtacaggt caaaagcagt tgatcagtat tgctcgaacg atcatcacaa
     8401 atccatcttt attgattttg gatgaagcaa caagtaatgt agacacggta acagaagcaa
     8461 ggatccaaaa agcaatggat gaagcaatca aaggaagaac cagctttgtg attgctcacc
     8521 ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt gcgtgatggg gaagtcattg
     8581 aagaaggcag tcatcacgag ttattagcag aaaatggatt ttatgcggaa ctttatcata
     8641 atcaatttgt atttgaataa agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa
     8701 cagccaatca aaaacagctt ctcatgtttt tgatttggtt ggacttatta ccgcagatat
     8761 aagcttttga gcaccgtgaa taaagaggtt atgaaatcaa cgctctaacc tgagtattag
     8821 agaaaaaaac gcaaaacata tcatttatgt tttgcgtttt ttatttatct ttttttctca
     8881 atacaatata aaataggcgg ctggttcttt tggttgataa accgataatt cagcacatta
     8941 tatgtttcct gtggcagttt ggaacaaaaa gaatcaacca tatccagttc tttttctcca
     9001 ccttcatgac catagtagac gacaaggatc aatcgtccgc gaggcacaag tcgtttcaat
     9061 atttcttcca tagctgtgcg tgtagtctct ggcatagtga tgattgcttt atcacttttt
     9121 gggagatacc ctagattgaa aatccctgct ttgagatttt gttgttcagc tatcacttct
     9181 cctaacgttt cgtggccttg tagaaacaat gagacacgtt cttctaactg acgctgttct
     9241 agcctttcac gagtagcatc gattgcttgt tgctggatat caaaactata gacatgacca
     9301 gtttttccga tgtgttctgc tagaaaaacc gtatcgtggc cattgcccat cgttgcatct
     9361 acgacgtgat cccctggttg caagatttct tgcagtaatg tatggctgaa atgcaaagca
     9421 gtttgcaaca tcagacgctc acctttttcc gtacatcata ttttccttgg aagctatctc
     9481 tgcgaagaag ttcttcatca attgcattca agacttccca ttttttcaag ctccacattg
     9541 gtccaatcaa ggaatcccac ggcgcatccc ctgtcaaacg atggatgatg atctcttttg
     9601 gtatcatttc cagctggtcg caaatgacat tgacatagtc aggacggctc attaattgaa
     9661 ggcgaccttc atgatagtcc cgcaacattc tcgttttgcg catcagatgc atcaaatgga
     9721 gcttgattcc ttggatatct gaatctaaaa tggtgcgtct gacattttct ttcatcattt
     9781 ctaaactttc gcctggtaat ccgttgatca aatgcgtaca aacacggata ttatgtttgc
     9841 gtaatttagc cacgccatct aaataagttt gataatcatg tgctcggttg atcagttttg
     9901 aagtttcttc aaaagttgtt tgcaatccaa gttctaccca taaatacatc cgctcgttca
     9961 gttcagcaag gtaatcaact acttcatctg gcagacaatc cggtcgtgta ccgactgata
    10021 atcctacgac acctggtaaa ttgactactt gctcaaatcg ttctttgatt atttcaactg
    10081 gcgcatgggt gtttgtaaaa ttttggaaat aaacgatgta ttgttggaca tgaggccatt
    10141 tttgatgcat catgtcgatt tctttctgga attggattgg aagagggtct tcaggtgcaa
    10201 cgatcatgtc tcccgaacca gatacactgc aaaaagtaca accgccatgg gcaacagtcc
    10261 catctcgatt cggacagtca aatcctccat cgatcggcac cttgaaaatt ttcccgccaa
    10321 actcatttcg taaagcgtaa ttccaagaat gatatctttt atttccttct gaataaggaa
    10381 aagtcattaa aagtccttct ttctgtaaga gttatctatt tgttttcttt tttctctata
    10441 cactggataa gtcaggcaca gccaagaaag actgagtaag taaccgccga tgatatcagt
    10501 aggaaaatga acgcccagat aaatgcggct tatcccgaca cttactataa taaacatcat
    10561 cagcaattga agcaatgtgc gtaaggttct tttttgaaca aaagctggaa gcagaaatag
    10621 taaagaaccg taaagaatca tactgcctgc tgcatgtcca ctagggaaac tgtaacttgt
    10681 ttcaactact aagtgcgcca atgaaggacg ctctcgcatg aaaaatagtt tgatcaccgg
    10741 gttgatgatt ccagcaatta caataaatcc agaaacgaac caaaatgttt ctgccctttt
    10801 tttcttatac cacatccata aagccgcaca agcaaataaa atgatgacgg taagaggatt
    10861 accgaattta gtcataaaga gttggtagct gttcagatca ggataagaag ctctgacaat
    10921 gcctgtaaac cactgatcga aaccagaaag ccacgacaga tggaacttta cgatccagcc
    10981 caaaatgaaa aacaggacaa gaaagaagct accagccacc tgaaatgatg ttttatattt
    11041 catacaattc ctcctttgaa gcaatgactt attatagcat ttttggtcca gcgaaaaaaa
    11101 cggtaaagag ataaaaccaa tgtatgattc ctctttgaat aaccaatatt cttataaaaa
    11161 aaataactgt aatcggaaag acattttttg gtaatgcaaa tgaaatgtta gggaaataaa
    11221 atcagtgttt ttcgaaatcc ttttaaacaa gaacgtagac gtaggaagaa cttgaaaatg
    11281 aacagaagtt gttcattacg gtaaattaca atatgattac aaaaaacaac aaagctctta
    11341 aataactttt aataaatatt aagagaagtt ataattatta ataatagata aattgaagtt
    11401 ggaggataga agatggaaaa cattgcaaga aaagaacgga gacgtttaga acagaccaaa
    11461 cgttttcgaa aagcaaaacg aggggcagca atcgtgggaa ctgcgatggt aggctgttct
    11521 gtcgctgccc cattagtaca gccggttcct gttcaagcgg atgaaacacc tgttcagttc
    11581 ggcgcaagaa tcaatacagc ggcatttatc acagaaattg ctacttatgc tcaaccaatc
    11641 gctcaagcca acgatctata tgcatcagtg atgatcgcac aagcagttgt agaaagcggt
    11701 tggggcagta gtgcgttatc tcaagcacct tattataatc tttttgggat aaaaggcagc
    11761 tatcaaggac aaaccgtcta catggatacg cttgaatttt taaataatca atgggtaaat
    11821 aaaaaggagc ctttccgaca atatccttct tttgctgaat ctttcaatga caatgcctat
    11881 gtacttagaa atacttcatt tggcaacggt tattactatg ccggtacttg gaagagcaac
    11941 acgaaatcct atacagatgc gacagcttgt ttgacagggc gttatgcgac tgatcctggt
    12001 tacgcaggca aattaaataa tatcatcgca acttacggat tgacccaata tgatacacca
    12061 gctagcggaa acgcaggcgg tggagcgact acaggtggca ataatactgg aagtgcaggt
    12121 agtactggcg gttcaagcaa ttctggtaat acaaactcag gttcagcaac ttcgaccact
    12181 tatactgtcc aatctggtga ttctgtttgg ggaatctcaa ataaattcgg tatcacaatg
    12241 gatcaattga tccaatggaa caacatccaa aacaatttca tttatccagg acaaaaactg
    12301 acaatcaaag gtagccagga aaatggctct tcgacgaata attccggtaa caataccaat
    12361 tcttcaggaa atgctggtac atcaaacgga ggacaaacaa ctggtgcgaa atacactgtt
    12421 caatctggtg attctgtttg gaagatctca aatgatcacg gaattacgat ggctcaattg
    12481 attgaatgga acaatatcaa gaacaatttc gtttatccag gacaacagct aatcgtatca
    12541 aatggcggtt cttctaatgc tggagcaaat actggctcaa atactgggaa tacaagtggc
    12601 tctagttcgt ccagctcaag ttcttctaat acagcttcag gaacgaaata tacagttaaa
    12661 gccggagact ctgtttggag cgtctcaaat aaatacggaa ttacgatggc tcagctaatc
    12721 caatggaaca atatccaaaa taactttatc tacccaggac aacaactgac tgtttcaaac
    12781 agtggatcaa caaacagcac gtcaggtaat acaggttcgt caaccaatac aaataacagc
    12841 agttcagtta atactggcag cagttcgtct caaggcactt ataccgtgaa agcaggtgaa
    12901 tctgtttgga gcgtggcaaa taaaaacggc atcacgatgg atcagttaat cgaatggaat
    12961 aatatcaaaa acaacttcat ttatccagga cagcaattga tcgttaaagg tggaacttcg
    13021 gcaaatacaa atacaggttc aactacttct gcagcgaaac caaatacacc gaatacgtct
    13081 gctacgacaa gtacatcatc cactggtaat acgatgtata ccgtgaaagc gggcgagtca
    13141 gtttggagcg tagcgaacaa acatcatatc acgatggatc agttaatcga atggaataat
    13201 atcaaaaata atttcatcta tccaggacag caagtgattg tgaagaaagg ttcatctcaa
    13261 tcaacaaacc aacaatcagt tactggttcg aaaacctata ctgtgaaatc tggagaatct
    13321 gtttggagtg ttgctgacag tcatggaatt acaatggctc aactaatcga atggaacaat
    13381 attaagaaca actttattta tccaggtcaa acgttgattg tagctaaata aaatagctgt
    13441 tcgaagaaat aacgattgaa ttccacaaaa atatttgtta ctataaggct acttaaatat
    13501 gacaacactt gaaaaggaag tagtaaaaag actgaagttt cagagagcgc atggctggtg
    13561 agaatgtgca cggatgcttt tgaactcgcc tttgagttgc ccttgctgaa tacccgagta
    13621 agcaagcggc ggtgacggcc gttacaacgt tcgaggcctt ttcactaagg taaaattagg
    13681 tggtaccgcg tagattacgt cctataaaaa gagcaatctt tttataggat ttttttattt
    13741 cttttgtgaa gtttgagaaa acaaagcaaa aggaaataaa aataaacaga ttctgccggt
    13801 tttttcgtta tatttataat agaaacaagt gttttcaata gaacaggagg acagcaaatg
    13861 agttacaatc ataaagaaat tgagaaaaaa tggcaaaaat actgggcaaa aacgaatact
    13921 ttcaacaccc atgatgatcc tgaaaagcca aaattttacg cactggatat gtttccctat
    13981 ccatccggtc aagggctaca tgtagggcat cctgaaggtt atacggcaac ggatatcctt
    14041 tcacgtgtga aaagaagtca aggatacaac gtgctgcatc caatggggtg ggatgcattc
    14101 ggtctgcctg ctgaacaata tgcgctagat acaggaaacg atccagctga gtttacccaa
    14161 aaaaatatcg aaacattccg acgccaaatc aattctttag gatttagtta tgactggaat
    14221 cgcgaagtga atacaactga tccggagtat tataaatgga cacaatggat ctttacgaag
    14281 ctttatgaaa aaggtctagc ttatgaagca gaagtagcgg taaactgggt acctgaatta
    14341 ggaacagtta tttcaaatga agaagtgatc gatgggaaaa gtgaacgtgg tggctatgat
    14401 gtagtcagaa aaccaatgcg ccaatggatg ctgaaaatca ctgcttatgc tgatcgctta
    14461 ttggatgatc tagaattagt tgattggcca gaaagcatta aagaaatgca aagaaattgg
    14521 atcggtcgtt cagtaggagc aaatattgaa tttaaagtgg ctggtacgga taaatcctat
    14581 accgtattta cgacacgacc agatactcta ttcggtgcaa cttactcggt tcttgcacct
    14641 gaactagatc ttgttagaga aattacgacc cctgaacaaa aagctgctgt agaagcttat
    14701 atcgaagaga cagctaaaaa atcagatcta aagagaacgg atcttgcaaa agaaaaaaca
    14761 ggagtattta ctggtgctta tgcaatcaat ccagttaatg gcaaggaaat cccaatctgg
    14821 atcgccgatt atgtattatc ttcatacggc acaggagcca tcatggctgt tccagcacat
    14881 gatgaacgtg actttgaatt cgctcaaact ttcggactag aaattcttcc agttatcgaa
    14941 ggtggagatg ttgaaaaagc tgcttacaca gaagatggta cgcacatcaa ctctgaattt
    15001 ctagacggca tgaacaaaca agaagcaatc gataaaatga atgaatggct agaagaaaat
    15061 ggcgtaggga aaaaagaagt cagctatcgc ttacgtgact ggctattctc ccgtcaacgt
    15121 tactggggtg aaccaatccc aatcatccat tgggaggacg gaacagtcac agctgtccca
    15181 gaagaagagc taccattacg gttgcctaag acaaaaaaca tcaagccaag cggtactgga
    15241 gaatctccat tagcaaatat cgaggagtgg gtcaatgtcg ttgatcctgt aacaggtaaa
    15301 aaaggccgtc gcgaaacgaa tacaatgcca caatgggcag gaagctcatg gtattacctg
    15361 cgttatatcg accctcataa caaaaaagaa ctagcagatt atgaaaagtt gaagcgttgg
    15421 ctaccagttg acatctacat tggtggagca gaacatgcgg tacttcattt actatatgca
    15481 cgattctggc ataaattctt atacgatatc ggtgttgttc ctacaaaaga accattccaa
    15541 aaactataca atcaaggaat gatcttaggc gaaaacaacg aaaaaatgtc aaaatctcgt
    15601 ggtaatgtag tcaatcctga tgatgtggtc gaaacttacg gtgcagatac actgcgcatg
    15661 tacgaaatgt tcatggggcc attagatgct tcgattgctt ggagcgaaaa tggcttagaa
    15721 ggaagccgta aattcttgga tcgtgtatgg cgtttgatcg ttgatgagaa caataaaatg
    15781 cgtgaccgta tcactacatt gaatgatggc aagcttgata aagtgtatca tcaaacagtg
    15841 aaaaaagtga cagaagacta tgaaaatctt catttcaata cagcaatctc ccaattgatg
    15901 gtctttatca atgaagccta taaagtggac gctctgcctt atgaatatat cgaaggattt
    15961 gttcaactac tcgcaccgat tgctcctcac atgggtgaag aactatgggc aatccttgga
    16021 aatgatggtg gtatttctta cgcaccatgg ccaacatacg atgaagcagc acttgttgaa
    16081 gacgaagtag aagttgtttt ccaagtaaac ggtaaagttc gtgcgaaaag caatgttccc
    16141 cgagacttag gaaaagatga gctagaaaaa gcagcattag ccaatgaaat agtgcaagaa
    16201 tatattgaag gcaaaacagt cagaaaagtt attgcggtac caaacaagtt agtcaatatc
    16261 gttgctaatt aattggctct ttgtcaataa ggactgatga gttgtgcaaa atcaaatctg
    16321 ggccagaatg aacttcattc tgtctcagat tttttgttat ttcaaattga ttaattgatt
    16381 gatcaaaaag gttttgattt tcatgttctc aaacacgttc aatgtctttt tcttctgaat
    16441 ggtcttgtaa gagatctctg taatcatcaa atttttgtct attaatctta gtattttttg
    16501 atgtataaac tacctgaaat tgagagtaca taccaaattc ataaattttt tattagtggg
    16561 actagtgatt gtatggattt tgacgcataa tgaatgaagt atgagaaaag gagagacaaa
    16621 tggatcaaga actatttatt atcgaagtat tatcagctaa acaaaaaatt atcgagaaag
    16681 cgaaaaaaag agcgaaaaga ataatgttca agcttttaag cttagtattt ttctctggtt
    16741 tgcttttttt cggagctgta tcgttcctat atcgccatat gagtgcagta gtccgttcag
    16801 ctgaagggat gcaaaaatta aataatttgt atcacttttc gtggggagtt ggcggaataa
    16861 caattgttgt gcttattgtt tttcttctac ttatttggct ccgttaccat caattgtcgc
    16921 gtcatttcga tcaatggctt agtcaaagag cacaacagtt tttacataat aaaccaactt
    16981 ctcaagatga tgactatctt tattttgaga agacttcgac gagtcaagct tttcggatac
    17041 aaaaaagtga ttgccagtta ttgctgagat cacttgatga tttacctatc tacttaggcg
    17101 aaagcaaatc aaaattcgga atgaacacat tccatatttt tttactagaa aacgaaccca
    17161 gatgggaaaa aagccagtct tcgcctggtg gatacctgac aactaagatg agtacattag
    17221 tcactggttt tttcctcatt cttttagctg gaacaatcta ttatgggaga tacactcagc
    17281 caaaaatgtc ttctcatata aatgctgctt ctcacatgac aatgaactca actaacgata
    17341 cgcatataca aaagacaaat gaagggttgt tagctgatcc taagcaaaag gtaaatttga
    17401 caacacaaaa gataaatgat ctcaaacttg atacagagac gcactctctc tatatgacaa
    17461 cagatagtgg tgctaattgg gtatttgtgc cgattgattc gcaatggttg cgttttggcg
    17521 aatacaccct gacaacgggg ctgattcctg aaggttattg gatggacaac acatatgata
    17581 tttccgccga tttctcatgg tttatctatt cttctgggga tgatgtacgt ctgctgactt
    17641 caagagataa tggtaaaaca tggcaaacgt ctattatcac ccaatcagtt cagccgatac
    17701 gttatcgaaa agcggtattt tatggcaacg atggtctatt tatctgtaca tcagccagtg
    17761 ggatgtctgc agaaactttg tacatttaca ctactatgga tggcggaatc acttggataa
    17821 aaagtggaag tacaatgatc aatcagccag tacaaaatgt cagcttcgtt acaacttctt
    17881 taggttttgt ttcgacacgt gaaagtttgt tttatacaaa caacggagga cgctcattca
    17941 aagaatcagt tctgtcgatt ccggaaaact atcagacagg ggggatagat ctttttcaat
    18001 caccaaatga agttactcaa ataagcgcga atacgttaga gacaaaattt tatttgttga
    18061 aaaaaggacc aatcgatgca gggaaaatgt ttgcctgcct cttccgttca actgataacg
    18121 gggaaacatg gcaattcgtt cagcaactat cgcaagtaga acaaggagat taaagaagag
    18181 agattgtgag cctgacgttc agcaccgagt cacaagaaac aaattcaaaa aacagcttct
    18241 catatttgtt gaatagtgta actcgtgatt cggagttgac aagagcaaca agaagcgatt
    18301 ttgaagtata gcttctcgta ttttacaaga tcgtgtctta ttgccgatag gttgtctctt
    18361 gaacaccgtt tattcgtaaa aagggattgt gacaagtctt ttgtcactac ttaccgaacg
    18421 aaactgaagg agctatccaa aaagtatcat tagtattatt ctttgtgttt ctagtggcac
    18481 cactagtaca tttaaaaaag ctatcgatca tggactaatc atacatgatc gatagctttt
    18541 tagataatgt ttgttacgct atctgaaatt gactattata aagactggca taaagcgttg
    18601 gtgattggag tagttcagaa tgtgttcctt tttcgacgat atcgccattc ttcattacaa
    18661 ggatcatgtc tgcattttca atcgttgaca aacggtgtgc aatcacaaaa cttgtcctat
    18721 cttttgtcac agcttccatt gcttgctgaa tcaatgcttc tgttcgtgta tctacgctag
    18781 aagttgcttc atctaaaata acgacaggag gattcgctag aatgactcga gcaattgtca
    18841 ataattgttg ctgtccttga gataacgtgc cgttttcgct agaaatgatc gtatcatatc
    18901 cgtctggcag tgttcgaata aagtgatcac attgtgcgat ttcagccgct tcgataattt
    18961 gttctcttgt ggcatccatt ttcccgtagg cgatattatc agctaccgtt ccttcaaaca
    19021 gccaggtatt ttgtaaaacc atcccaaaag aacgacgtag ttctccacgt gaaagcttgg
    19081 taatatcaag accgtcgaat tcgatctttc ctttattcag ttcataaaaa cgcatcagca
    19141 gattgactaa agtcgttttt cctgcaccag ttggaccaac gatcgctacc gtttgttttg
    19201 gctttactga aaaatcgact tgattcatca atattttatc tgatgtgtaa ccaaactgta
    19261 catttttgaa agctattttt cctttcggtt gttctaaaag tcttgcatct ggaaggtctg
    19321 gaatttcatc tgtttcatct aatatctcaa atatcctatc gattgctgcc attgcggatt
    19381 ggatcgagtt gatcacataa gaagcggtag aaattggttc agaaatctga ttgatgtact
    19441 gaagataagc ttgtaagaag ccgatcgtca caccacctga tagaacaagc atagcaccga
    19501 ataccgcact tatgatgaag gcgagttgat taatgaaacg aatggccgga tagatagcaa
    19561 agttcataaa ttgagcacgt ttaaaagcgt tgtattgctt tttatttact tcgtgtatag
    19621 cagcttctgc atgagcttgc tgattgaatg ttttgaccac tagattacca gctaagattt
    19681 cttccattcg attatttaaa acacccagtt catactgatt ctgttcagaa atctgtttgt
    19741 ttttgttcgc aatcttagtt gtaacaattg aactgccaac gatcagcaat aagacgagca
    19801 aagtaagctt gctttcgata taaaacagca tcagaccagc agcagctatt gttacgacag
    19861 aagtaaaaaa ttgattgata cctgtcaata acacttgaga aagttgattg agaccggtag
    19921 ttgtccgact aatgatgtca cctacttgat gattgtcaaa aaacgacatc ggtaatgttt
    19981 tgaatttttt tgtgatttct tttcgcaccc tcaatgtgat cttttcgctt aacgctgcca
    20041 ttgtccgttc ctgaataaat gaagtgatgc tgcttaagac tgaacagccg atcagcaata
    20101 agacagggaa caacaatgct tgtttgatat cactgaaaga aagctgtcca atacctgttt
    20161 ttcttatttc tgataagaga tcatcgatcc cgattcccat gatcaagggc atggctacga
    20221 ctaataggtt cccgaataaa ctgcatagaa acaatccgta aaaatattta tgttcaggtt
    20281 tgatcatttt ccaaaaacgt atcaaagcac gcgtgttcaa cgtttttttc ttttccatcg
    20341 tatcccttct ttctatattc cttgtgattt tgcaaactcc tgataagttt ggcaattaga
    20401 cagtagttct tcatgagtac cttgtccaac gatttttcct tcatctaaaa caatgatgtt
    20461 atctgcttgt tggattgtac tgaggcgttg agcaacaatc acgagagttt tctccgacat
    20521 gtgtttatga agggctgctc ttaatgctgc atctgtttta tagtctaaag cggagaaact
    20581 atcatcgaat acatagatct ctgctggttt gatcaacgct cgagcaatac atatccgctg
    20641 tttctggccc cctgaataat tagaaccgcc ttgagcaaca aagctgtcca atccgtcagg
    20701 aagggtgtcc actagttctt ctaattgagc gatcttgatc gctttagaca actcttctgt
    20761 tgaggcatat tcatttccca tctgcaagtt gcttaaaatt gtcccgctga ataagaaagc
    20821 cttctgaggg acataactga tgtgggagcg gatctgttct tgagtcattt gagtaagagg
    20881 aataccacca agggttactt ttcctatcgt agggtcacta agtctcaaga gaagtttcgc
    20941 caaactactt tttccagatc cggtcccgcc aacgatcgct gttgtttttc ctttaggtat
    21001 tgtgaaatca atatcttcta gaactggatc agagatttca gtatatttaa atgtgacatg
    21061 ttcaaaagca gctgcatttg tctgatcaaa ggttggttca gctactgttt tgatcgtctc
    21121 ttcctcaatt tcgatctctg cttgcagtat ttcttctatc cgtcgtaatg aggcaaacga
    21181 tttagggata atgaccaata ccatagctgc gatcatcaag taagcaagtg tcagcataga
    21241 gtattcgact acagcagtca cgacaccgat ttgtaatgct ccttctgcta caagatttcc
    21301 gccaaaccac aaaatagaag cataaacgat tcccatgagc aaaaaagcaa ttggagtgac
    21361 ccaagcaaat attcggttca ctttgatcat ttgattagca tagtcagcaa agctcttatt
    21421 cgttcgcttt tcttcatatg attgattatc aaaggcacgg atcatattaa tcccagtaaa
    21481 gaattctctc aatgtgatgg tgatccgatc catttttggc tggatactca aagataaagg
    21541 tgcccctcgt ttcatcagtc gataaacaat ccatacataa aaaacaacag agatcaatgt
    21601 aacccatgtt agtaatagag aataacgaaa catcatgaga atggtgaaaa cgcaaataac
    21661 cggtgctggt aagatcattt ggcaaaataa aacaatcatt tgctggacat tatcgacatc
    21721 attcgtcatt cttgtcaaaa gagatccaga actaaaacga ctgatatcct tcattgacag
    21781 ctgttggaat ttacggaaat atttctcccg cgtttggaat ccaaacctag cggaagccaa
    21841 tgctgaaaag taactagagg caactgcagc gattgttcct agaaacgcta caagcagcat
    21901 ttcaactcca atttttttta taactgaggc ttcgccactt gctatacctg tgtcgatcag
    21961 ctttgctact aattttggta cacctaatgt accggcgatt tgtaaacaga tcatcacaaa
    22021 ggtaaggaga gcgagtcctt tgtttttctg taagaatgtt tttaagatag acatattatt
    22081 cctccttttt tcctgatact tttcgattct ataccttcaa gtaacttgaa ggtcaataga
    22141 aaaaagaata aactgaaaaa gaggtgagaa aaaatgaaaa aaatgtatac gattggccaa
    22201 gtatcggaac tatttgattt gccaaaatca acgatccgct attgggatga acaaggatta
    22261 atccgttcaa gtagacaaga agagaatgat tatcgattgt ttgatatcga tgatattttt
    22321 atgctttatg atatcgtttt ttatagaaaa ttagatatac cgatcaaaca gatgaagaat
    22381 ttatatggaa aaacattgac ggagttatat gagacacttg atgaaacaga aagaagaatc
    22441 cataaagaac tggtggtgat gaaacaaaaa caaaaagaga taagggagcg aaaaaaacag
    22501 ttgaaattga tgatcgatac aaacgaggaa gaatttcctg tagaagagat cccttttgac
    22561 tgtatgatct caacggagtt tgaagatatt gtggaaatca aaaaatttct acctaactat
    22621 tcaagctttg ggatgatgag tatgccagct tccagttcac agacgatgta tggatttttt
    22681 atcgacccta gtgaagtaca tttatttact caagacgtga tttgggagaa aaaagatacg
    22741 gctgtttatc gtcgattctt attgaaatcc gagatgaacc atgcggaacg taataatatt
    22801 ttagaaatca gagaaagaat gtatgaaaaa ggatggaaaa caggagaagt gatcggccag
    22861 tatctattga cgaatacgga tgaaaacaat atcagaactg aatattatca tgcatggata
    22921 gaaatgaaaa aataggaaat atgtgtttaa gataactcga aaatgatggt tgttttagtt
    22981 ataaagaaaa attttctggt tcgattacgg gtttaatgtg ggggattatt agtcgtggat
    23041 agatagaatt ttagtttttt agaggaaata tgtctttaaa aatggaatgg ttgcaattct
    23101 tagcgatata tacgatgtaa ttgtcctctt aaaaaagtat ggaaaaataa ttgtattggg
    23161 tgttatgtat aattcatggt gaactgagac taatgagaag aaaaattcat attgttatga
    23221 gtgagattgt atcagatgag aatgataaat taaataggtg cataaaagca ttacacttaa
    23281 aatacattgt tgcttacatt attacaatat gttaataagg caaaaataaa tagatggatt
    23341 gaataaacat attttttaat caaatagtcc ctactgtgac tttcctcagc tatcggtgat
    23401 actaccgata gcattttt
//
LOCUS       DAZUKC010000040        23360 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000040 DAZUKC010000000
VERSION     DAZUKC010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.041"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(270..1766)
                     /gene="lysS"
                     /locus_tag="ACG80T_001528"
     CDS             complement(270..1766)
                     /gene="lysS"
                     /locus_tag="ACG80T_001528"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFS4841176.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1877..2881)
                     /gene="dusB"
                     /locus_tag="ACG80T_001529"
     CDS             complement(1877..2881)
                     /gene="dusB"
                     /locus_tag="ACG80T_001529"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFS4841177.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2882..3769)
                     /gene="hslO"
                     /locus_tag="ACG80T_001530"
     CDS             complement(2882..3769)
                     /gene="hslO"
                     /locus_tag="ACG80T_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFS4841178.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3986..6097)
                     /gene="ftsH"
                     /locus_tag="ACG80T_001531"
     CDS             complement(3986..6097)
                     /gene="ftsH"
                     /locus_tag="ACG80T_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFS4841179.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6187..6732)
                     /gene="hpt"
                     /locus_tag="ACG80T_001532"
     CDS             complement(6187..6732)
                     /gene="hpt"
                     /locus_tag="ACG80T_001532"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFS4841180.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6732..8177)
                     /gene="tilS"
                     /locus_tag="ACG80T_001533"
     CDS             complement(6732..8177)
                     /gene="tilS"
                     /locus_tag="ACG80T_001533"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFS4841181.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8297..8767)
                     /locus_tag="ACG80T_001534"
     CDS             complement(8297..8767)
                     /locus_tag="ACG80T_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFS4841182.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8813..9238)
                     /locus_tag="ACG80T_001535"
     CDS             complement(8813..9238)
                     /locus_tag="ACG80T_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFS4841183.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9305..9574)
                     /locus_tag="ACG80T_001536"
     CDS             complement(9305..9574)
                     /locus_tag="ACG80T_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFS4841184.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9585..11180)
                     /locus_tag="ACG80T_001537"
     CDS             complement(9585..11180)
                     /locus_tag="ACG80T_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS4841185.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11195..14716)
                     /gene="mfd"
                     /locus_tag="ACG80T_001538"
     CDS             complement(11195..14716)
                     /gene="mfd"
                     /locus_tag="ACG80T_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFS4841186.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVSDQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14733..15293)
                     /gene="pth"
                     /locus_tag="ACG80T_001539"
     CDS             complement(14733..15293)
                     /gene="pth"
                     /locus_tag="ACG80T_001539"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFS4841187.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15646..16620
                     /locus_tag="ACG80T_001540"
     CDS             15646..16620
                     /locus_tag="ACG80T_001540"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFS4841188.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16790..18190
                     /locus_tag="ACG80T_001541"
     CDS             16790..18190
                     /locus_tag="ACG80T_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFS4841189.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18268..18990)
                     /locus_tag="ACG80T_001542"
     CDS             complement(18268..18990)
                     /locus_tag="ACG80T_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4841190.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19069..19575)
                     /locus_tag="ACG80T_001543"
     CDS             complement(19069..19575)
                     /locus_tag="ACG80T_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFS4841191.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19698..21497
                     /locus_tag="ACG80T_001544"
     CDS             19698..21497
                     /locus_tag="ACG80T_001544"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFS4841192.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21565..23139)
                     /locus_tag="ACG80T_001545"
     CDS             complement(21565..23139)
                     /locus_tag="ACG80T_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFS4841193.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT         6395 a         5056 c         4253 g         7656 t
ORIGIN      
        1 ttttgttatg tttttgaata aatattttct tctgtctttt ggacaagtaa taatatacca
       61 actaaattta gcttggtcaa cgaatttttt gtattttttc aagttttttt caataaacga
      121 ttattcataa cttttctttc tttatcgttc gttttcgtct tctttaatgt ctagaatagt
      181 cggcattagc ttttgaactt tcgttattat ttattccttt ctacacagca aaagctgcct
      241 aaggaattcc ttagacagct tttattcgat tatctcattg ttgggaatag caaaacatcc
      301 cgaattgatt gtgcatcagt tagcaacatt actaatctgt cgatcccgat gcctagacca
      361 ccagttggcg gcataccgta ttctaaggct tccaagaagt cttcgtctac accgtgagct
      421 tcgtcatttc ctagttcacg ttctttttct tgcgcttcga atctttctct ttgatcaatt
      481 ggatcgttca gttctgtaaa ggcattagca aattcgcgcc ccactatgaa tagttcgaat
      541 cgatctgtga atcgaggatc ttctggattt ttcttcgcta aaggcgaaac ttcgactgga
      601 tgtccgtata caaacgtcgg ttgtgtcaat gtttcttcaa caaatgtttc aaagaattca
      661 ttgataacat gtccgacacc catagcttcg gttacttcta cattgtgttt ttcggctaaa
      721 gctaatgctt cttccaatgt catttcttgc cagaagtcta caccagtttg ttctttgatt
      781 gcatctacca tatggatgcg tttaaagtca ctttctaagt cgacagcttg tccatcatat
      841 gtgatttttg ctgttcctag tactttttca gctgcgttac ggataatacc ttctgtcaga
      901 ttcattacat cattgaaatc agtatatgct gtgtatgctt ctaacatcgt aaattctgga
      961 ttatgtgtag tatcaattcc ttcattacgg aaaacgcggc cgatttcata aactttttcc
     1021 attccgccaa caattagtcg ttttaaatga agttctagcg caatacgcaa atataaatca
     1081 atgtccagtg cattatgatg cgtgataaat gggcgtgcgg cagctccacc tgcctcattg
     1141 tgcaatactg gtgtttctac ttcaatatag ccattgccat ctaaatagcg acggatttcg
     1201 ctgataattt ggcttcgttt cataaatcgg tcaaagcttt cgcgattact gattaaatcc
     1261 aagtagcgtt gtctgtagcg ttgttcaata ttcgttaagc catggtattt atctggcagt
     1321 ggacgaagtg cttttgacaa aatcgtgatt tctgaagctt tgatagaaac ttcgcctgtg
     1381 tctgttttca tgatttgtcc agttacacca aagaaatctc caaggtctgc atgtttgaat
     1441 acttcatagg cctcatcacc tacttggtct tttcttacat aaatttgaat ttggccttca
     1501 cgatcttgta gatgcgcaaa tccagctttc ccttttcctc ggcgagtcat catacgacca
     1561 gctacactag cggtcaatcc catttctgct agttcttctt ttgttcttgg atcaaatagt
     1621 tcatgcaatt cttctgagtt gtgcgtgcgt tcaaatcgtt ttccaaatgg atcgattcca
     1681 tcttcacgca attgttccat tttttgacga cgtacaagca tttgatcatt taaatcttct
     1741 acttgatgtt gttgttcctg tgtcacaaaa gttcctccat tctcatgtta atctctttcc
     1801 tataatgcca atgaaacaca aaaaaagcaa gccgaacttg gaatttcggc ttgaaaattc
     1861 acgaaaaact tagaaatcac aacgtttgtt tcaatagtac tttttccgct ttttctacaa
     1921 attgatccag caggtcattg atttcttgct gtttttctgt ctgattgatt gctactttca
     1981 ctttcgctgc tcgaggaatc ccttttaaat aataagccgc atgtgttctg aattctctac
     2041 aagctacttt ttctcctttg agatctgcta agcgttgtaa atgaagttta gctgtagcaa
     2101 ttttttcggc tggtgttggt tcaggaatca gttcccctgt ttcgagataa tgctgtgtgc
     2161 ggtgaatcat ccatggattg cccagcgctg cacgaccaat catcacaccg tctgcaccga
     2221 catagtcaag catccatttt gcatcttctg gcgttttgac atccccgttc cccataaatg
     2281 gaatcgtcaa atgttttttc acttctttca atacttccca gtccgctttt ccttcataca
     2341 tctgaacccg tgtacgtcca tgcatagcga tggcacttgc tccagctgat tgtgctgcta
     2401 atgcattttc tacagcaaag acgtgctctt catcccaacc aatacgcatc ttgattgtta
     2461 ctgggatatt tactgctgaa gaaacagctt ggaccatttc gtatactttc tcaggatcta
     2521 aaagccattt tgctcctgct tctgctttga ttactttatt tactggacag cccatattga
     2581 tatctattat atcggcagct gtgttttctt cgacaaattg agcagcttca actagactat
     2641 ctttatttcc gccaaatatc tgtaaactta atggatgttc tgtttcttca atgtggagca
     2701 tctctaaagt ttttttgttt ctgaaatgaa taccttggtc actgatcatt tcacatacga
     2761 ctaagcccgc accaaattct ttgacagtga ctcgaaatgc tgaatttgtt attccagcca
     2821 tcggtgcaac aacgacacga ttagggattt ccacattgcc aattttccat gtgtgttcca
     2881 cttaaatccc tcctaaggct tctttcttca atccatctaa ttcttcttcg ctatacttgt
     2941 atttgttatt acagaaagaa caaaccgctt ctgcaccgtg atcttcgtct atcattgctt
     3001 ggatttcttc tgcacctaac gcgataattg cttctgcaaa tttttctttt gaacagtcac
     3061 atttgaattg aacaggcatt ttttccaaaa tgttcaattc ttctgtacca agcaatcctt
     3121 tcaatatttc ttctggtgtc tgtccttcat ctaagagggt agagattctt ggtgtcgttt
     3181 ttaattgttc ttcgatcttg ctgattgttt cttcatcggc acctggcatg atttggatca
     3241 tgaatcctcc cgccgtcttg atgctgtcgt cagtattgac caatacactc agtccaacag
     3301 ctgaagggat ttgttctgag atagcaagat agtaagtaaa atcttcagcg atttctccag
     3361 aaacaatcgg cgtttgccca gaaaaaggtt cttttaatcc taagtctttt gtgacagtaa
     3421 acattccttg tgttccaact gctccccgca catcgatttt accttcacta tttagaggaa
     3481 gactgagatg cggatttttg atgtaacctt tcacatctcc ctttccattt gcatctacta
     3541 caattcctgt tgctggacca tcgccttgga ttcgaacagt tagtttgtct tctcctttga
     3601 gcgtcgcacc aagtagcaga ctaccgacca atgtccgtcc caaagcagct gaggaggtgt
     3661 gccaagtatc atgacgtctt tgtgcctccg ctacggtatt tgttgcttga acggcgtatg
     3721 ctcggacgaa tccgtcgtga gcaagtgcct taaccaaata atctttcatt tgttatgtac
     3781 tcctttttcc gtttttaaac tcatagtgaa tcatactagc aactactgct tttttcaact
     3841 ttggaatgct tcttttaaaa ataattcaga atcatctaga aaacaggaca tcaccctatt
     3901 tacaacttaa ataaagaaat ctcccgaaaa ccggtttttg ttccggtttt cgggagtcat
     3961 atcagatcaa ttgacctata tttttttatt tgtattgatc gtctgaatga ttatcttctg
     4021 attgttgaga atctgaatca tgttctgctt tattttcctc attcagttca tgttttgctt
     4081 cttcaaaatc acgtttttct tccgcttgtt tttcagcgtc tttttcttcc aaagcacgtt
     4141 ttgcttcttc aaaagttttc gcttcttttt cgcttggata atcatttgac tcagtacctt
     4201 caggcatcac accgtgttca aacaatgact tgattgcttt ggcatccaat gtttcgaatt
     4261 ccaaaagttt ttcagcaatc agtttatgct gtgcacgatg agcttcgatg atttctcgag
     4321 ctttatcatg agcttccatc aagatttttc gtacttcttg atcaatctcg aaagcgactt
     4381 gttccgagta agcttttgtt tgaccatagt cgcggccaac aaacacttga tggtttcctt
     4441 catattgaac aggacctaaa cgatcactca ttccatattc tgttaccatg cttcgtgcta
     4501 gagctgtcgc ttgctcaaag tcattggaag caccagttga ttggacattg aagatgattt
     4561 cttcagctgt acgtccacca agtaagccta cgatttgttc aaacatatct tctttcgtca
     4621 tcaagaactg atcttctttt ggtaaagcga tcatatatcc gccggcgcgt ccgcgaggga
     4681 tgatcgttac tttgtggacg acccgagcgc gactcaatac taatccgaca atcgtatgac
     4741 cagcttcgtg ataagcaacc atttcgcgtt cttttttgct gattacgcga tcttttttag
     4801 ctggacctgc aatcacgcga tcttccgctt catcgatatc agaagcatcg attttctttt
     4861 tatttcttct agcagctact aacgctgctt cattcaagac attttctaaa tctgctccgg
     4921 caaaacctgg tgtctgttga gccacaactt tcaagtcaac gtcatctgcc atcggtttat
     4981 tccgtgcatg gactcgcaag attgcttcac gaccttttac atctggacgt ccaactaaaa
     5041 tctggcggtc aaaacggcct ggacgtagta acgctgggtc tagtacatca gaacggttgg
     5101 ttgcagcaat gacgattact ccttcatttc cgtcgaagcc atccatttct acaagcaatt
     5161 ggttcaatgt ttgttcgcgt tcatcatgtc cgccgcccat accggcacca cgttgacgac
     5221 ctaccgcatc aatttcatca atgaaaatga tagctggtgc gtttttcttc gctgtttcaa
     5281 acaaatcacg aacacgactt gctccaacac cgacaaacat ttctacgaaa tcagaacccg
     5341 agatcgagta aaacggtact ccagcttctc cggcaacagc ttttgctaat aaggttttac
     5401 ctgttcctgg aggtccttct aaaagtacac ctgccgggat acgggcacct aattcaacaa
     5461 atcggcgagg atctttcaga aattcaacaa cttcaactag ttcttgtttt tcttcttccg
     5521 cacccgctac atcagaaaaa cgtacgcgat tagctttttt gtctgcttct ttggcttttg
     5581 atttaccaaa gttcatgaca cggccgccac cgccaccgcc gccgccttgt tgtcccatca
     5641 tcatgtaaaa gaaaaagatg atgattacga ttggcaagaa gctaagcaat agggaaaccc
     5701 atataccgct gcttgattct tcttttactg ttgtagcaac tttgttttct tttgccagtt
     5761 ctgttacttg gctcaatgtt gtatcatttg gcaaaatgat tgttgtgaaa tgggtagtag
     5821 tcgtatctgt tgaacctaag atagctaacc caccgctgtt agcgacggtc tgctgttctt
     5881 tgtattcacc agtgatctta tatacaccat ttgcaggctg cacttcaaat tctttgatct
     5941 tgcctgcttg tagttgttct gtgaattttg agtactcgat atctgacgat cgctggccgt
     6001 tattaccaaa aatgaaataa acgaccatta ccatcgctaa gattacgagg acataataca
     6061 aggcattttt catcatgccg ttgttctttt tgttcatagt tgccctccct aagcgtcaat
     6121 ttaatttaac agttttttat caatcctgac cattctattg taccataatt taattgagat
     6181 acagcattaa tttgattgat aaacactagg tttcaataca ccgatgtacg gaaggttacg
     6241 atatgcttct gcataatcca agccataacc gaccacaaat tcatttggta cgttgaagcc
     6301 aacataatct gcttcgatat ttactactct tccttcaggt ttgtcaagta gtgtaacaat
     6361 tttgactgat tttgctttgc ggtatttgaa aagatccact aaataagcaa gtgttcttcc
     6421 gctgtcaatg atatcttcaa cgatcaacag atcgcgacct tctacattcg tatctaagtc
     6481 tttgacgatt tttacttccc ctgacgagac tgttgcattc ccgtagcttg aaacatccat
     6541 gaaatccaat tccaaatacg tatcgataga acgaacgata tctgccatga atggtaccgc
     6601 tccttttaag acacctacta ctaggggatt ggtgttttgg taaatttctg ttaactcttt
     6661 gcctagttct ttgcaacgaa cttgtatctc ttcttgtgag atcaaaattc tctcaatatc
     6721 cttttccaac atcaggttct ccttccaatt gcttcttttt gatatttgta aagaagtatg
     6781 tagtgtattt tatcagtttc ttcacgaata ctcaaatatg aaaaggcaaa aggtatcaag
     6841 ctatatatat ttcttttggc atctgtaatg atccaagcat gctgcctttg tgaattaggt
     6901 actttctgat cgataaaata tctagatact ttttttgtga gattctctgt taatttaatg
     6961 cggtctcctg cttttctttt ttcgacatac agttgcacat cagcaggaat ccaaatgtca
     7021 tgagaaaata gagaccatga atctgttgct ttgatgtccc ctgcattttc aggtttgacc
     7081 agaccaatcc attctgtttc attcaaatat attccttgtt ctggaatcag caacaagcgc
     7141 tggtcttctg tctggctagt tgcttgtttt tcttttttag agagtacaac aaaatgatac
     7201 tctcttttca gtatccaatt attgccaata ctgagttgcc attggctttt tttctgttgc
     7261 cactggtcta aaatcgactc catttgttgt tctttcaaaa taattcctgt ttttgagaat
     7321 gctttgtcga aaaaggagta aaaaaaatag tatcgttcag catctgtcat ttcttcaatc
     7381 atttctgcag aaaatcgaaa ttgttcctcc cactgttcga cttccttttc gatcaactgt
     7441 cccatatatt tttgaatgac ttggtctgcc cattggatct gttgcgaaaa ttgctggaaa
     7501 tgtctcaacg cctgtgtatt ttcttgtttc aattgaggaa ctaccaaatg acgaagccga
     7561 tttctctgga tatctaacaa ttggtttgtc tgatcctcaa agtaaacaag ctgcgattcc
     7621 gctgcataag tatataggtc ttctttgcta aaactcagca acggacgaat cagtctgccc
     7681 gtagcaaatg gttgtacttc tttgattcca gaaaaagtat ttaactgtcc cccacgaacg
     7741 attttcatta acaccgtctc gatttgatca tctccgtgat gagcagtcat caacgtgtca
     7801 tattgtcctt cttccatcac gcgagtgaat tcttgataac gaaagttacg agcttctgtt
     7861 tctatggcgg atttggctgg atcttcccaa atacttacat ataaagggag attcttctct
     7921 tcacaatatg agcgaagata ggcagcttct tctgctgatt cttctcttaa ttggtgattg
     7981 atgtggacga tacctaaaga aaaaccgatt tggcgttgca cttgtaaaag taaatggagc
     8041 aaaaccatcg aatcaacacc acctgatacc gctgctaaaa ttctcgatcc ttgttgccag
     8101 aatccttgtt cattcccgtg ccgaataaat gactgctgta atagcggtgc ttttttcccg
     8161 cctttttcaa cgttcatcct ttccacaccc tttcttccta aaaaaaagag agcgggaaaa
     8221 cgtctaaaac gacaattaac ctgccctctg ttgatactat gacccaaata gtcgcactat
     8281 cattatttgc ggctttttag ctgcgacgtc cgccacggcc accgcgcttt ccttctgtgt
     8341 ttcgcttcaa tgaagataag cggtcatcgc tatctttcaa aaatgaactc atcaatgaat
     8401 cgaagtcttc tttttgagct gaaggttgac tgcgagaaaa agattttttc tgtgctggac
     8461 gatttcggtt gtcacgggga tctttttgtt gatatttttt aggacggctt tcttctccag
     8521 attcagcagg ttgttcctgc gctttccgga tagatagtcc gatttttcca tcatcattta
     8581 ctgaaataac tttaactgtt acttcatctc ctacacttaa aacatcatgg atgtctttta
     8641 catagccatt tgacacttcg ctaatatgaa ctagacccgt ttttccagca cctagatcaa
     8701 taaacgcacc aaaatttgtg attcctgata cttttccttg cagttttgct cctacctcga
     8761 ttgacataaa aaaaatgttc ctcctaatta tttccctttt ttatattcct gcttattttg
     8821 cagttgtaga actagttgat tgatctgttt catttgtcga gctgtctgta tttgattcta
     8881 atataggata aactttttca ccgtcttttg agtaaaagaa tttgcttctg gcaagtttag
     8941 ctacatactc atcatctttc agcaatgaaa catcttggtg cagctgatca acgtctgcgt
     9001 tgacttcttt catttcagta tttgcttcga ctctcaattc gttcaattga tgcatccttt
     9061 gcatgtcatg gaaaatctgc acacctatta tcgcgaaaat gaccaaagca ccaacaaaaa
     9121 ttgcagccag tctgcgtctt cgaaaaatca gttgacgttg ttgttgctga aattcagcat
     9181 actttttctt tgcatattcc gtatctagcg cagcgatttt attgcttttt ttcgtcactt
     9241 ggattcgctc caatcttttt gattgtcttt ttcattatac caatatcgtg aaagcttgtc
     9301 taatctattt ctctagtgtt tctgtgcgat tttcagaaat aatttcgtac attttttgtg
     9361 catcttcttt tttggttgat tcatgtaatt ccgtaatttt cacttccaac gttttgttgc
     9421 caaactggac tttcaaaata tcgcctactt tgacatcact ggaagatttc gccaatttac
     9481 cgttgatttg aattcttcct ttatcagcta cttcttttgc tactgatcgt cttttgatga
     9541 ttcgggaaat ttttaaaaat ttatctaatc gcatatctta ttcctcactt tttaattttt
     9601 ttatttaatc gcaggatttt cttaccaaaa ggcaacatta gccattctcg tactgtaaac
     9661 agacgaagac gaatcactgc ataaatgaac acagaagccc cgatcccaac tcctatcacg
     9721 ctgacaaaaa aagcagtgga acgatgagtt acaggaccaa atactatatt caatacacca
     9781 taatacagca gcagtactac tgtcatacaa agaaggctga acatcaactt ccgtccaaaa
     9841 tgccgttctt tccaaaaagc atttagctct tttgtttcaa ttcgaacaaa ataccaaagc
     9901 gtcacaccta atcctaaaag ggtagacagg cttgcacctg ctgttcctaa taaaccggtc
     9961 agaaaagaag tagtcaatcc ttttaccaat agtccccatc ctgcgccttt taaagaaggg
    10021 cgaaacgagt tcttgctttg agcaatactt tgataagctt ggatgactgc agtgaaagca
    10081 atggaaaaaa caaataatac aagcgctgca tttcccgcat aatctttaaa caacgcataa
    10141 ttgatataag ggagcagcaa ggctaatccc aaagacgcag ctaatgctaa agcggtcgtc
    10201 aatctaagat agattttggc tgtctgtaaa aactgacgat agatccggtt ggtcaaatga
    10261 cgagtcaacg caggcaggaa tgtcgcactt aatgcagtgg caatcactaa acctaattgt
    10321 actaaaggct gacctcgatc atagacccct tttgctatct tcgcaccgta ttctgacagt
    10381 ccgtaaactt ccaaagcatt ttttacaaag aaagagtcga tcagctggaa aaagataagc
    10441 aatccactat aaatcgaaac cagtccgcct tctaccagaa aacgccggat caagggcttt
    10501 gacggctgtt tgaacaatgg aaaccgtcgg aagcttaacg ctcctccatg aatcttttgt
    10561 tcatagtagt gaaggatccc atatgcgcat aaaccgccaa aaacagctcc actcattgct
    10621 gccgtccctg tttggtacac cgtccatcca agctttctaa aagcaaacgc actacagaca
    10681 atcacaaaaa cacgaacgaa ctgctcaact acttgagaaa cagcagttgg ttccatcaag
    10741 aatcttcctt ggaaattccc tcgataaaaa gatagaccag gcatcagcaa aaaggtaaag
    10801 gaaactactt ggatcagcgg tttcaattgt atgtctccca tcatccaagc aatccagctg
    10861 ctaaatgcaa agacaaacaa ccacaaaaag acacctgtcc aaaatactaa gggataaaga
    10921 ttttgcaaag cttgctttcg ctcgaaagga ttctttcttt ctgcaacgta ctttgatatg
    10981 aactgcggaa gaccggataa agctaaagtc attgctatcc cataaattgg ataaacttgc
    11041 tgataaacat aaaaaccttc gtcgccgact aagttttgga ggggaattcg gtaaagggca
    11101 cttaatactt ttgctatgaa agaagcaatc gtcaatacaa atgctccttg catgacgcgc
    11161 cgcatttctt tatgatccaa atcattgctc cttcctacgc tgacagatat ttttgctcac
    11221 gcaacgcttt gacaaacttt tggatttcct gaagccatac tgcttcttcc atttggttgg
    11281 ggatcgtcaa gcgtatcacc atctgttctt tttcaacgcc tagactggct tttagttttg
    11341 tcgctgtcag tgcttcgaat aactgttcca cactatagcg ttttgttcca actttactta
    11401 gagtaaagac tatatgcgcc tcctgtttcc ttatcgtttc taggagcgct cgatcgccat
    11461 ccattttgat ctgcccaatt gtcaagagat gtgctacttc ttctggatat tctccaaaac
    11521 ggtcgagcag atcatcttct agttcatcta gcatttcttg cgaatcaagt tcacgaattc
    11581 gtttatagat ctcgattttc tgccgttgat ctgacacgta tgtttcaggt agatacgcat
    11641 cgacaccgag gtcgatctca acagtggtct tctccacttg actattcttt ccttgtttac
    11701 gagaaacagc ttcgcttagc atttgagaat acatatcgaa accgactgca tcaataaatc
    11761 catgctgctg agcacctaag agattacctg caccgcgaat ggaaagatcc cgcatcgcaa
    11821 tcttgaatcc agatcccagt tctgtaaaat ctttgattgc ctgaagacgt ttttcactga
    11881 cttcgttaag gatcttctgc tgctcataca taaagtaagc atatgcgaca cggctgcttc
    11941 ttcctacccg ccctcgcaat tggtataacg tggataatcc catataatca gcgttctcaa
    12001 cgaacaacgt atttgcgttc gggatatcta ctcctgtttc aatgatggtt gtagtgacaa
    12061 gaacatcata ttgaccttca ataaattcaa aaagcgtatt ttctaattgc gcctctgtca
    12121 tttgcccatg agcataaccg atccgtgctt ccggaacgag ttcttggatt tcttctactt
    12181 tttgttcgat cgtttctacc cgattatata aatagaatac ttgtccacct cgtcccattt
    12241 ctcgatggat tgcttcgcga atagccccag gatttttttc cataacatac gtttggatcg
    12301 gataacgatt cgcaggcggt gtttcgatta ctgaaagatc ccgcacgcct aacatcgaca
    12361 tatgcaaagt tctcggaatc ggtgttgcag tcagagtcaa aacatccact tgtgcacgta
    12421 actgtttcaa tcgttcctta tgttttacac caaaacgttg ttcttcgtca atgatcagta
    12481 gacccaaatc gctaaattca atatcttttg acaaaattcg gtgggttcct acaacaatat
    12541 caacttgacc tgtacgcagt tgttcgatgg tttctttttg ttgctttctt gttctgaaac
    12601 gacttaatag cccgatattt acggggaaac cttcaaatct ttccagcatc gtttcatagt
    12661 gctgctgtgc caagatcgtt gttggaacta aaaaggctac ttgtttgctt tcctttatcg
    12721 ctttaaaggc cgctcgaagc gctacttctg ttttcccata accaacatca cctacgagta
    12781 gtcgatccat tggtttttct ttttccatat cccgtttgat ctccgctgca cttctcaact
    12841 ggtcatcagt ttcagagtag gggaaggcat tttcgaattc tttttggtag gcgtcatcag
    12901 gtccaaaagc atatcctttt tctgactctc ttgcggcata taatttgatt agatcgtcag
    12961 cgatatcctc gatttttgaa gagacttttc gcttggtttt cgtccattca cttccgccta
    13021 gcttattgat gcgaggcgct ttggcttcag aggcgacata tttttggatc agattcagtt
    13081 gagtaacagg aataaacagt ttgtcgtcgt tttgatacaa aatagtcata tagtcttggt
    13141 gaacgccatc tacttctagc gtttccatcc caatgtattt cccgatacca tgattggcat
    13201 ggacaacgta atctcctgct tttaactcat tatagctttt caatcgctct gcgtttgtta
    13261 ctgtttgacg tctcgtttgt tttttcgtcg ttttttggaa aatttctttt tctgtgatag
    13321 ctactatttt ttcttgagga agttcaaaac cagcctgcaa agctccttca actaattgta
    13381 cctgtcctct gatcaatgca tttttttctg tttctactac taatatgtct tcatctcgaa
    13441 gcatttgttc tgctttgcgt attctgtcct ttgtcggaat aaagatcaca acagtctgtt
    13501 tttgcttgcg ccaacggtcc atttctgttt tcagcaatgg catttgtccg aagaactgtt
    13561 gcatcgtccg atattgaaaa ttatggattg cttggaaacg aagatttccc atcccttttt
    13621 ggaagagtga gaaaaaggtt gtggtgaact tttccttctg cacgattttt tgtatatctg
    13681 ctgaaaatgt ctgctcagaa aacacccgca attcgctgat tttttgagtc tgccattctg
    13741 ccgcttccct ttcgatttcc cgatttgttt ccatgattcg ctgataatca tcaacgacta
    13801 gtaaactatt tgaagggaaa taatccaaga tcgtcacaga ttcttggtaa agaagatctg
    13861 agtagaattt tgcttcttct gtcgggacac ctgcttgcca gcttgaagtc agctgaccga
    13921 agtaatcttg caaaaactcg cggtctgctt cttctgtggt cgcagcaagc cgtttttcta
    13981 gcattttttg catttgctct attccatgtt ctagatcctc tggtccgtag acttgttcac
    14041 tcgttgggga aacccagatt tcttccgttt tttcgattga gcgttgtgtt tctgcttcaa
    14101 aataccgcat agaatctatc tctacatcaa atagttctac acgaacagga taggttgtcg
    14161 ttaacgggta aatatcgata atgcttcctc gaatactgaa ttctcccggt tttgcgacca
    14221 tctcttctcg tacatagccc atcaaagtca attggcgagg aagattttcc acgtctatct
    14281 catcaccaat atgccaattc aattgcgcct gtagccaagt tttcttattc ggcagtcgtt
    14341 tgtgcaaaga tgcgactggc aacaaataga tacctgcttt tttttcttga agtgcgttaa
    14401 gcgttgcaac tctctcgcct cgcgcttctg gagaagaaaa ggccatttca gcagaaagca
    14461 cttcatccac tggaaataaa tggatctgat cttgcggaat cacatgttgc agatcttctg
    14521 ctaactgatt cgcataatac aaatttggca tcagaataat catgttttta tttgttttct
    14581 taaacgctcg agcaaataga agtgttttcg ctgagcccgc taatcctgtg attagttgtc
    14641 taggcatttt ttcttctaaa ttgctgaccc attcttttac ttgcggatct tttccgacta
    14701 attgaataat atccattcct tttcctcctt cctcaattga atcgattcat cgcatccacg
    14761 aacgaatgcc cttctatgag atattcgact gctttaacag attgacacat gctttcttct
    14821 atcaaaggct gattttcttt gctgaaagga ctaagcacat gctggactac tgtttttttc
    14881 ccttctggcc tgccgattcc caccttgatt cgatcaaaga cattcgtatt caaatgggaa
    14941 ataatacttt tgataccgtt atgcccgccg gcacttccct tttgacggag ccggattttt
    15001 cccactgcca gatccaaatc atcgtagatt acgactaatt cctctggata aatcccaaaa
    15061 taagtcatta gtgggccgac tgcacgacca gattcattca tgaatgtttg tggtttgact
    15121 aaaaggatct tttctccatt atgaaaaaat tcggcaactt ctgcttcgaa cggatttttt
    15181 ttgaaagtag cttgatgctc gcgtgcaatt ttatccacga ccataaagcc gatattgtgt
    15241 tttgtatatt ggtatttggt acctgggttt cctaacccga cgatcatttt catttttgtt
    15301 ttccacctaa ttctaaaaag cagcacaaaa ggctggactg ctaattagcg gtccagtcca
    15361 tcgagctttt ttctttttta cacttgtcaa tgcatagtat aacacaaaac aaggcgtttc
    15421 ggctaaaaga gaatcgttaa taaatgataa agaaattaaa aatttcttca aagtttaatg
    15481 aaataattga tatataaaaa tagtgaaata acgcacatgt aagcttttac atataataca
    15541 accttgttat ctgttatatt ttatagccaa atagcgtgac atggggcttg agaaatgtta
    15601 gcataagcat gggaaaaaac aaatcaatgg aaggattgat actaaatgac tgcagctgca
    15661 gaaaaaaaag atcatcaaaa agttattctt gttggagacg gcgccgtagg ttctagctat
    15721 gcctttgctc tagttactca aaatatcgct caagaagtag gtattgtgga tatcaatact
    15781 gctaaaactg aaggagacgc aattgacttg tctcacgctt tggcatttac ttcacctaaa
    15841 aaaatctatt ctgctactta cgcagacgca catgatgctg acttagttgt tattacagct
    15901 ggcgcaccac aaaaaccagg tgaaactcgt ttagacttag taaacaaaaa cctaaaaatc
    15961 aaccgcgatg ttgtcacaca aatcgttgaa tcaggattta acggtatctt tttagttgcc
    16021 gctaacccag tagatatttt gacttactca acttggaaat tctcaggttt ccctaaagaa
    16081 cgcgtaatcg gttcaggtac ctctcttgac tcagctcgtt tccgtcaagc acttgcggaa
    16141 ttagttgacg ttgatgcacg taacgtccat gcttacatct taggtgaaca cggagattca
    16201 gaattcccag tttggtcaca tgcaaatgtt gccggcttgc aaatctatga atgggtaaaa
    16261 aacaacccag aaatcgatga agaagctatg gttaacctat tctttggcgt acgtgacgct
    16321 gcttacacaa ttatcgagaa aaaaggcgct actttctatg gtatcgcagt agcattagca
    16381 cgtatcactc gtgccatctt agatgatgaa aatgctgttc ttcctttatc agtattcatg
    16441 aatggcgaat atggattgaa cgacatctac attggtgcac ctgctgttat caaccgtcaa
    16501 ggtatccaaa aagtaatcga aattccttta agtgacagcg agcaagaccg tatggctgct
    16561 tctgctaagc aattaaaaga aattctagat gaagcttttg ctaaactaga cgctgaataa
    16621 tctttgaaca tgacggaaaa catctattcg gtgttttccg tttttttatt ttatcaccct
    16681 tatggtttct aaattttttc ataaagtaga gataaccttc gtcttttcct ctaaattatg
    16741 ctatcatgag accgttccac cctaaacttt tacttaagga gttgtcgata tgacaagatc
    16801 ttcgcatcga aaaaaatcga aacatgttgc tttgctttca gttcttggtg ttctcttagt
    16861 gctgatcagc ttttatacct atcgcagtac atactacaca aagcattttt tgccgaatac
    16921 agaaataaat ggaataaatg tcagcaatct aacagtcgaa aaagcaaatg aaaaattaaa
    16981 agaagcttat tctgataaaa aactggctat taaagaagat ggaaaagtct ggcaggaagt
    17041 accaaaatcc gagctcggct acaaagatga tttcacttcg gagctttctc cgatcctaaa
    17101 tgaacaaaac ggatggactt ggggaatgac ttatgtctca gcggcagaaa aacaagaaat
    17161 agatcctgtc agtcaaaacc accaaaaact agacactacc gttcagacat tgacaactaa
    17221 gctgactgat ttgaacaaag accggacacc tactactgat gcaacgatcg aaaaagcagg
    17281 tgactctttc gttatcaagc ctgaagtcaa tggaaatacg atcgacgtcg atgctgcggt
    17341 caaacaatta aaaagtgcgg taaactctgg taaagataca attgaattga ctgaattcaa
    17401 agaaaaacca aaagtaacat ctgaagacag ctcactgaaa gaacaactcg catccatgaa
    17461 tgctatcgca aatgtaaaag ctacttacag tataaacggt gagactttcc agatcccatc
    17521 aagtgatatt atgagttggc tgacctacaa tgatggaaaa gtcgatttag atacagaaca
    17581 agtacgtcaa tatgtaacag atttaggcac aaagtataac acaagtacga atgacacaaa
    17641 atttaaaagt acaaaaagag gagaagttac ggtcccagtt ggcacttaca gttggacgat
    17701 ccaaacagac agtgagacag aagcattgaa aaaagcgatt ctggcaggcc aagattttac
    17761 tcgttcaccc attgtgcaag gtggcacaac cgctgatcat ccattgatcg aagatacgta
    17821 tatcgaagtt gatctggaga accagcatat gtggtattac aaagacggaa aagtggccct
    17881 tgaaacagat atcgtttccg gaaagccaac tacacctaca cctgctggag tcttctatgt
    17941 ttggaataaa gaagaagatg caactttaaa aggaacaaat gacgatggaa caccttacga
    18001 gagtccggta aactactgga tgccgattga ctggacaggt gttggtatcc acgattcaga
    18061 ctggcaacct gaatacggtg gagacttatg gaaaacccgc ggttcacacg gctgcatcaa
    18121 caccccacca agtgtaatga aggaattatt cgggatggtc gaaaaaggaa cgcctgtatt
    18181 ggtcttctaa caaaaataat atataaaaag aaagcacccg tacgattggt acaggtgctt
    18241 tctttttatt atctaacaat gtgctactta catttcagca gcagtaaaga cttctgaaac
    18301 atcatcatcg tcttctagct tatctactag acgttccaat tgctcttttt gttcctcatt
    18361 taaatcaatc gtcgtttgtg gaatcatcgt caattctgct tgcgccaaag tgaactcttt
    18421 ttccaactca tctcgtacag cagcaaaatc ttctggtgca gtataaatct cgaatacttc
    18481 aggagaagtt tgcagatctt ctgcccccgc ctctaataca tcctcaaaca tcgtctcttc
    18541 atcaacagtc aacccatcgc gttcaatagc gatatatcct ttgcggtcaa acaagtaact
    18601 gactgaaccc gtttctccca gtgatccacc gtttcgagta aaagctactc ggacatttgt
    18661 cgctgtccga ttacggttat ctgtcaatgc atggactaag attcctactc cacccggacc
    18721 atatccttca tacgtgattt catcgtaatt cgtttcatct acggcactcg tagccttttt
    18781 aatcgcccgt tccacattgt cgttaggcat atttgcagct tttgctttgt ccacaacaag
    18841 ccgcaaagct ggattcatag atggatcagg accgcctgct ttagctgcca tataaatttc
    18901 acgagaaagt ttctgaaaga ctttccctcg ttttgcatct tgggcatttt ttcttccttg
    18961 gatattatgc catttactat gtcctgacat gatttttcct cccttgttct tttgagaata
    19021 cacttctatt ctagtactat ttcacaaaaa aacaaagcca cagccaatct ataaacgctt
    19081 cttgaatgcc gcaaaaagca cacataatat cacagcagtc agagcaccga tcgaatccaa
    19141 catgacatct tggaacatag gtgttcgtcc accggtcatc atctgatgga attcgtccaa
    19201 acctgcgtac cctgttgctg aaagccaagc aaggatcgct gtcagccacc aaatttttag
    19261 cttggggaaa acacctaaaa acaagctgcc tcccaaaata aaataggtac cgaaatgtgc
    19321 gcccttcctt aaaaagaact ctaaaaattt ggcgtagccc agatggttga tactcaccat
    19381 acttccacca taattaaaac gtatacccgc taatgtgtct ttaaatggct gattaggcag
    19441 ccagctttct aatcgcgaaa cttgtgattg ctgtccgtaa gtttgagaag aactgtaaaa
    19501 taagattata acgactatca gtgcgattac taaataaata tttccgtttt tcatttgttt
    19561 ttttgaagaa ttcataagtc acctctgtat tttatcttag actttttttg aagaaacaac
    19621 aatcattttc atagagactt cttccataaa aaagtgctat acttaatttt gtattacaat
    19681 atgaagggag ttttttcatg agttattcat taaattggaa tctagattct attttttccg
    19741 gaggcagtca ttctgatgct ttgaaccaac ggatgaagca attggaagac caaatgaacg
    19801 agtattatca ccgcgtaaca aaatggtcac ctagttctga caatgcagag cagttaaacg
    19861 ctattcttca attgcaagaa acgattacga atggttttac ccaatgcagc agctatatta
    19921 ctgcattgct ttctgccaat gtcaatgatt cagatgcaaa ggtcttatca ggcaagcttt
    19981 acgcaatgct gcctcgtctg caatcagccg aaactgtatt gtcaaagaaa tttgcagaaa
    20041 tatctgacaa tgattggaat cagatgcttt ctcaatcttc gtttgaaaca attgcttttc
    20101 gtttaaacga gattcgtcgt gatggctcac aattattatc agaagctgaa gaaaacatta
    20161 taaatacgct ttctttagat ggtctaaatg cttggagcag tcattatgat acgattgtag
    20221 ccagcatttc gattcccttc gaacaagatg gacaagtagt cgaattatct gctggacaag
    20281 ccttcaataa gatgatgggc gatccagatc caaaagtcag agaaacactt ttcgcagcat
    20341 gggaagaagc ctggaaagaa aaggcacccc tcttcacgga tactttgaat catttagatg
    20401 gttttagact ttctgactat gaacttcatg gtgtcacaga ttttctacaa aaaccattag
    20461 agtacaatcg gttgaagaaa gaaacattgt ctgtcatgtg ggatacaatc cagaaaaaca
    20521 aacaacccct tgttaattac ttgactagaa aagctaatct tttcggtaaa gaaaaaatgg
    20581 aatggcaaga tcaagatgca cctattattt taggcgattt gaaagaaaaa acctttagtt
    20641 ttgacgaagc agcagcattt attatcgaaa actttaacaa attcagtcca aaaatggcta
    20701 aatttgccca atctgctttt gaaaagagct ggatcgaagc agaagatcgt cctgggaaac
    20761 gtcctggtgg ttattgtact gagttaccag aaacaaaaga atcaaggatc tttatgacct
    20821 acagtaattc tgttaatgag gttgcgacac ttgctcatga actaggacat gctttccata
    20881 gcagcacgat gtgggattta ccttctttaa atcgcgaata tgccatgaat gtcgcagaaa
    20941 cagcaagtac attcgcagaa ctgatcgtag ccgatgctac attaaaagtc gcaaaaacaa
    21001 aagaagaaaa aatcaatttg ctagatacaa aactgcaaaa tgctttagct atgttcatga
    21061 atatccattc ccgattcatt tttgaaaatc gattctatga agcacggcaa gaaggattag
    21121 tctcagaaga taagatcaca gagatgatgg ttgaggcgca aaaagaaggc tatcaaggag
    21181 ctttagctac ttaccatcca tatttctggg ctgcaaaatt gcatttcttt attgatgacg
    21241 taccgtttta taacttccca tacacatttg gctacctctt tagtttgggc atctatgctt
    21301 acgccaacca aaagggagca aattttgaag aagaatatat ccatttacta cgcgatactg
    21361 cctcaatgac gacggaagat cttgcgaaga aacatttgaa tgtcgatctt acccaaccag
    21421 atttttggca agcaggtatc gacatggtga ctgaagatat ccaaacgttc atggaattga
    21481 cagaagaatt tatctgattt ctattttatc acaataaaaa aagagttagt ccagaatggg
    21541 ctaactcttt ttgattaatc attcttacat gctgacagca aagtcagggg caaaccattg
    21601 aactgaacca acttttacgc tttcgcctgg ttgaggagca tggatatatt gtcctccgcc
    21661 taaagcaatc gctacgtggt aagttccgcc ttgtgacccc cagaataaca agtctccagc
    21721 ttttgcttga gaaactgaaa tttttgttcc agctgattct tgaggaactg tccaaccacc
    21781 gatgtcacga ccagttactt gcatgtaaac atagcgtgtg aaccctgagc agtcaaagcc
    21841 acttggatct ttaccgcccc aaacatatgg agtaccaata tatttgtaag cttctgctac
    21901 gattgctgca ccattcacac ttccagaagg agctggagca ggtgttacag gttttggttg
    21961 ttcaggtgtt ggtgttggcg ttggagccgg agtactactt cctgtgccgt ttcctgtatc
    22021 aacactttga tctgttgatg gtgttgtagg cgcaggtgtt gttgattctt ctgttgttga
    22081 agattcaggc actgttgttg attcttctgt tgctgaagat tcaggcactg ttgttgattc
    22141 ttctgttgct gaagattcag gcgctgttgt tgattcttct gttgctgaag attcaggcgc
    22201 tgttgattct tctgttgaag attcaggtgt tgttgattct tctgttgctg aagattgtgt
    22261 agctgatgat gattcttcag tcattgaaga ttgtgtagct gatgattctt ctgttgctga
    22321 gctttgtgca gtagatgaag cttgagtagc ttgtgcttca gcctctgctt gttcgcgtgc
    22381 ttctttttca gctttttctt gtgcagcttg ttgacgagct tgttctgcta gacgttgttg
    22441 ttcacgaata cgagcttgct ctgcttcagc ttctgctttt tgacggttca agtcagcttt
    22501 cttgtcttca gcagttgctt gttcagcagc taatgaagtt ttcagtacgt tcaaatcagc
    22561 ttgtttagat aaaagatcgc ctttttgaga ttccaaagca gcttggtttt cagctaattc
    22621 ttttagttta gcatcgtttt cagctttttt gtcttctaca gcttttttat cttgtttttg
    22681 ttgttccatc aagtcgttgt ttgcttttac catcgttgtc attgcttgaa cgcgtccaat
    22741 agcatctgct aatgaatcag cattcaatac agcatcgatg tagtttgagc ttgtgttgct
    22801 tacttgtgct tcgcgtgctt gtttttggat tgtatcttca cgtttttcaa tacgttcttg
    22861 taaatctgca atgtctttta ctaattgtgc agattcttga cgcaatgtat cttgttttgc
    22921 taataggtct tgtgcttgtg tgttgatttc agaaacttgg ctttctaagg catcgatttg
    22981 tgattgtgca tctgcttgtt gatttttcaa gtctgcaatc ttttgatctt gctgttgtat
    23041 ctgagaatca aaatcgtccg ccgcggcagc gatcggtgat gctacggcag tcaaggtcat
    23101 tgaacatacc attactgctg atattaaact ctttttcact cttcattcct ccgactggct
    23161 taatttaata aatatctaaa tatatttaag tctttcttaa cgatataaaa attgtaccat
    23221 agcagtgtgt caacgatata atagttatgt tacaaaaatg tttcaaaaca acttttaaaa
    23281 atgacaaaac tgttcgtttc agaaaatgat catgaaaaag ccgcgttaag tctagcattt
    23341 cttaagataa caacgattct
//
LOCUS       DAZUKC010000041        23259 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000041 DAZUKC010000000
VERSION     DAZUKC010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.042"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..281
                     /locus_tag="ACG80T_001546"
     CDS             <1..281
                     /locus_tag="ACG80T_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841194.1"
                     /translation="FSVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQ
                     VNDINFNINARNVLVEEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            295..696
                     /locus_tag="ACG80T_001547"
     CDS             295..696
                     /locus_tag="ACG80T_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841195.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            698..1069
                     /locus_tag="ACG80T_001548"
     CDS             698..1069
                     /locus_tag="ACG80T_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841196.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1105..1407)
                     /locus_tag="ACG80T_001549"
     CDS             complement(1105..1407)
                     /locus_tag="ACG80T_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFS4841197.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(1665..1778)
                     /locus_tag="ACG80T_001550"
                     /pseudo
     CDS             complement(1665..1778)
                     /locus_tag="ACG80T_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(2161..3393)
                     /locus_tag="ACG80T_001551"
                     /pseudo
     CDS             complement(2161..3393)
                     /locus_tag="ACG80T_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            3647..4216
                     /locus_tag="ACG80T_001552"
     CDS             3647..4216
                     /locus_tag="ACG80T_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4841198.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(4394..4834)
                     /locus_tag="ACG80T_001553"
     CDS             complement(4394..4834)
                     /locus_tag="ACG80T_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFS4841199.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            4992..5756
                     /gene="map"
                     /locus_tag="ACG80T_001554"
     CDS             4992..5756
                     /gene="map"
                     /locus_tag="ACG80T_001554"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFS4841200.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            5788..6711
                     /locus_tag="ACG80T_001555"
     CDS             5788..6711
                     /locus_tag="ACG80T_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFS4841201.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            6787..7926
                     /locus_tag="ACG80T_001556"
     CDS             6787..7926
                     /locus_tag="ACG80T_001556"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFS4841202.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            7919..8719
                     /locus_tag="ACG80T_001557"
     CDS             7919..8719
                     /locus_tag="ACG80T_001557"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4841203.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            8719..9546
                     /locus_tag="ACG80T_001558"
     CDS             8719..9546
                     /locus_tag="ACG80T_001558"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4841204.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            9551..10258
                     /locus_tag="ACG80T_001559"
     CDS             9551..10258
                     /locus_tag="ACG80T_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4841205.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            10358..11224
                     /gene="rfbA"
                     /locus_tag="ACG80T_001560"
     CDS             10358..11224
                     /gene="rfbA"
                     /locus_tag="ACG80T_001560"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFS4841206.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            11238..11810
                     /gene="rfbC"
                     /locus_tag="ACG80T_001561"
     CDS             11238..11810
                     /gene="rfbC"
                     /locus_tag="ACG80T_001561"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFS4841207.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            11832..12860
                     /gene="rfbB"
                     /locus_tag="ACG80T_001562"
     CDS             11832..12860
                     /gene="rfbB"
                     /locus_tag="ACG80T_001562"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFS4841208.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            12958..13809
                     /gene="rfbD"
                     /locus_tag="ACG80T_001563"
     CDS             12958..13809
                     /gene="rfbD"
                     /locus_tag="ACG80T_001563"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFS4841209.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            13844..15877
                     /locus_tag="ACG80T_001564"
     CDS             13844..15877
                     /locus_tag="ACG80T_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841210.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            15921..17201
                     /locus_tag="ACG80T_001565"
     CDS             15921..17201
                     /locus_tag="ACG80T_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFS4841211.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYRWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            17411..18217
                     /locus_tag="ACG80T_001566"
     CDS             17411..18217
                     /locus_tag="ACG80T_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4841212.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            18229..19449
                     /locus_tag="ACG80T_001567"
     CDS             18229..19449
                     /locus_tag="ACG80T_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4841213.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            19439..21025
                     /locus_tag="ACG80T_001568"
     CDS             19439..21025
                     /locus_tag="ACG80T_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4841214.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            21064..23202
                     /locus_tag="ACG80T_001569"
     CDS             21064..23202
                     /locus_tag="ACG80T_001569"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4841215.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     ILEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
BASE COUNT         7412 a         3890 c         4794 g         7163 t
ORIGIN      
        1 cattttctgt aacagctgtg gtgtctgaga tagaggatac agaaaagata aaaatagtag
       61 aaattgaagt tttaaacaag aatgaaaaac aaattttttc tacgggtgaa gtatcggtaa
      121 gcttgcctcc tcaagttaac gatataaact ttaatataaa cgccagaaat gtcttggttg
      181 aagaagcagg agagcattat gctgttgtta aatttaatgg aactgagatt ggaaggcaaa
      241 tctttgatat caaggtcaac aaaccagtgg aaaaaaatta aggagacgat acggatgctt
      301 gatatagtac catcaaatac acatcggagt ggtagcttaa taaattttgc tgcgattttt
      361 gcttgcgcat cggtaattct agcatctcca gttgttgtgg atgcaagttc tactccgaaa
      421 actaaaaatg ataatcaact tgtaattact acaaaaaatg agattaacac aatttcaagt
      481 aatgatggca aaatttttga tgtgataaat acggttttaa aaagaaaagc tgaaatcaat
      541 ataaaatatg atgaagattt aaacttgtat ttctttgtta tcaaaacaac atctgaattg
      601 ttttcctcag attacgatgt tttggatact ctcgataatg tattatctga ctataaatat
      661 atggggaaat ctgtagtagc aactttgggg gaataaaatg tttgacttta atgaatattt
      721 atctatttcc aaacaaattg aatcagatga taaatataat tctaaagaat cttatagaag
      781 aactgctgtt tcaagagcat actacagtgc ttttaaaaaa tctgatgagt atttgaaaga
      841 aaattatgat attatttata atggaagtag cggaaaagga agccaccaaa ccgtttggaa
      901 tttgttttca acagttaagg aattgaatac cttaggaata cataatagtg ggtatagaat
      961 gttagagaaa agaaaatgtg ctgactatat tcctaatgaa accattacga aaacagatat
     1021 ggcgctgatg aatcgagaag cagaaaaaat aataaacaaa ctcacctaat gaatttcaag
     1081 cgagtttgtt tgttattttt ttatttaatc tttgtatctt tctggatcaa cgaaagtata
     1141 ctttatatag tcataacgcc gatgatcgct acgtgcgtcc ggcacgtcag tcacgatatc
     1201 aaacaaaaag tatacaccct tcttcattct agttttcgca gcgggaattt tgaaatagtt
     1261 cttattagaa tagtagagat tgattaataa gctatcttcg attgctaaaa agaaaacttc
     1321 tgaattccat actttataaa aatctttgat aaatctattc gaagggtcaa atttaaacca
     1381 taattgtgtt tttccttcca tcagcataaa agttcacctc aaaaagagta tacgaacgaa
     1441 tgttcttttt gtgaaggcgg aatttaatat actgaataat agaaaacaag tacttgtgcc
     1501 aaattatgtg ccaaaaaaat tcgaattcaa caaaatgtaa acaaatagaa aaccttctat
     1561 attaagattt cctgcaataa caaacacaac agaagacgtg taatagttag tcaggaacgt
     1621 acaaataacc cctgtatcct ttgcggtaca gggggttatt ttgtataagt agacattgta
     1681 gtttctaaac tgctatgtcc taaacgtttc gatacgctaa gtatatttac tccttgataa
     1741 agtaaaatag atgcatgcgt atgtcttagt ccatcaataa acagtcgaag agaaaatagg
     1801 gaaaaattga ttactaaaga aaaacttact aggtcgacgg tgtagttgat tttcatactt
     1861 caatagattc gaattaaacg atttcgattg gcttatacca gtcctatcca acgaaagata
     1921 tatcgttctt aactctagcg gtataacttg aagctttacg taaagccaat gggaatgaac
     1981 tggagatgac tttttattat ttttagtaat agctgatggg ttaatactta caactttcct
     2041 tttcaaagta ccatataaaa aaacggtggg gttgtttagc gaaataaagg atctaagcca
     2101 atagcttaga tcctttattc tatgctttat attatttttt gcatgtgtgc gaaagtatac
     2161 tcaagcccaa tcgccatttc ggaaaatcgg tactgtagag ccatcttcac ggattccgtc
     2221 gatatccatc ttgtcagatc ctaccatgaa atctacgtgg gtttgacttc tattcaatcc
     2281 agcttctgct aattcttcat cggacatttc tgtaccgcct ttcacactga aggcataagc
     2341 agaacctagt gctaagtgat tcgatgcgtt ttcatcaaat aatgtattga aaaagatgat
     2401 gccagattga gaaataggtg aagggtcagg tacaagtgcg acttctccta aacgacgcgc
     2461 accttcatct gtatcaagca gttttgctaa gacatcttct ccttgttcgg cagaaaaatc
     2521 aacaactttt ccatctttaa aagtaaattt catacccgag atgatggtgc cagcataact
     2581 cagtggcttt gtacttgaaa tgtaaccatc tactcgatga ctgtcaggtg ctgtgaacac
     2641 ttcttacgtc ggcatatttg ccatgaattt ttctccacga gcattatagc ttccagcgcc
     2701 ttcccaaaga tggtttttag gcaatccgat aatgatatct gtaccgggag cagtatagtg
     2761 aagggcagaa aattgttctc gattcaattc ttctgctttt tttgctaatt tttcatcatg
     2821 cttcttccat gctaagacag ggtcttcttc atagacacga gtcgttttga aaatctgatc
     2881 ccataatgcc tcaacttgtt tttcttctgg cagttctggg aagacttttg ctgcccattg
     2941 tttaccagcc gcagccacga cagtccaact gactttattt gcttgagttg ccttgcgtaa
     3001 attcattagt gctttgccat tcgctgattg ataagacgct actcgatggc tatctacacc
     3061 agcaaatgca tcgggatcgg cagaaacaac gctgatcctg cttgctcctt tttcaagcca
     3121 ttcatccgct tgatcgattt tagattgtgg tacattctca atacgatccg ttgcagcatg
     3181 taaaagaaat tctctttgga tctggtcgtc agtccattga acgataacct cagcggctcc
     3241 tagtttataa gcttcttgtg tgatcaatcg agcaaggggt gcctgttcca cgctgatctg
     3301 taaaacaact gtgtgtcctt tttctgtggc tacgccagtt tctgcaatta agcgtgcata
     3361 tttttttaga agttcattaa aatcagataa cataataaaa attcctcctc accatttttt
     3421 ctggtaacta ttagcatgat accatttaat tatgattcaa acaatgaggc tttaaataaa
     3481 aaaaggaggt tattttttta aggttaagtt tcatgatata tgttactact tgctatttca
     3541 gcttgtgaag aaaatcaaaa actaattttg catatcaaaa aaacatgtta caatgaaatt
     3601 aatcaatggt ggaagcgtta taaaaaaatt aaggagatta aaaaatatgg caagaaaaaa
     3661 gacgattact agagaacaga ttttggcagc tgcttatgaa gtagctgcca ccgatggatt
     3721 tacacgattt actgcgagaa acatcgcgaa caaaatgaat tgttccacgc aaccgattta
     3781 tcttgagttt aaaaatatgg atgacttgag acaagctctt tttgagcaga tctatgatca
     3841 tttacaatat gaagtgttca ctgttgagca tacagggaat acgattatcg atcttgcttt
     3901 taattatatc cattttgcaa gaagagagaa gaagttgtat cgttcactgt acttagaaga
     3961 atatggtgga ggaaaagaga tgcagaattt ctcttatact tactttgtga atacagtgaa
     4021 acgcgatcca gcttatgtga atctttcaga agagcagatc gaatctttgt ataatgggac
     4081 atggatcata gctactggga tcgcagcttt gatgtcttca agcatcatcc atccaactga
     4141 tgaacagatt gaaaagatga tccaagacag tatagatgct atcttgaaat tcaaagaacc
     4201 aatcgaaatc gattgaatat actcaaaagt tccacaaatc agtaggtaac aagagatgtg
     4261 tcaaaataag gaaaagttcg tttttcttac agcgtatgca tttttaactc ccgaagaact
     4321 cttccatgaa tgcaatgaat aaaagagcca tacagattga ataaatctgg atggctcttt
     4381 tattgcaagt atattaaatt tttgcgacta gttctttagc gaaagcttct aaattttcga
     4441 tatcttcttc ttcagcattc agatccactt taacattttc ggctcctttt gatgcgcctg
     4501 tttttgcaaa agcagcatcg aaatcatcca ccgatttgca gaattcatcg taaaaagtat
     4561 caccagatcc gcagacaccg taaatttttc ccaatagatc caattcttgc aagtcttcat
     4621 aaaagtctac gatctcatca ggcagatcgc catcatcgta tgtataagta gctactacgc
     4681 aaatatctgc ctcctcgaat tcatctgcat ctacttgggt acattcgttg atttcgactt
     4741 cgatatccag attttcaaat gcttctgcaa caatatcggc gatttcttca gtgtttcctg
     4801 tcatgctagc atatacgatt tttgctaaag ccatgatgtg cctcctcgtt tataattatt
     4861 taacaattat gaattatacc aaaacatcat tgaaaagaaa ataaaaccta aagacacctg
     4921 gggaatgtca tttggctaaa tgtatgccgc tatgttaaaa tgagagggat ttgtaaaaag
     4981 gagacgaaaa tatgattact ttaaaatcag cacgtgaaat tgaaatgatg gcagaatctg
     5041 gagctttatt agcagatgta cataaaaatc tgcgtgattt tataaaacca ggaatcacaa
     5101 gttgggatat tgaggtattt gtcagaaact atatcgagag tcatggcgga attgctgcgc
     5161 aaattggctt tgaagggtat gaatatgcga cttgtatcag tataaatgat gaaatttgtc
     5221 atggattccc tcgtaaaaag cctttgaaaa acggtgattt ggtaaaagtc gatatgtgta
     5281 tcgatttgaa aggcggcatt tctgattctt gttgggccta tgtagttgga gaatctacac
     5341 ctgagatcga ccgattgatg gaagtaacga agaaagcttt gtacatcggt attgagcaag
     5401 cacaagtagg gaatcggatt ggtgacatcg gtcatgctat ccaaacttat gtagaaggcg
     5461 aaaatctagc gatcgttcgt gattttattg gtcatggcgt tggtcctacg attcatgaag
     5521 aaccagcagt tcctcattac ggagaagctg gaaaagggtt gcggttgaaa gaaggcatgg
     5581 ttatcacgat cgaacctatg gttaataccg gaacttggaa aatgaaaatg gatcctaacg
     5641 gctggactgc gtatacaaga gacggcggac tttcttgcca atatgaacat acattggcta
     5701 ttactaaaga tggtccaaga attttgacat cacaaggaga agaaggcact tattgatcta
     5761 tccagtcaat aggtacggga gaggtacatg aaagtttgga acaaaataaa acaaaatgaa
     5821 aatctgatgc gctttattga gacgactcaa aagcgcatca ctgattcaga aatgagtacg
     5881 acctctgtcg tcgttgctta ttatttactc ttgtctttat ttccattgat tattgctttt
     5941 ggtaatattt taccgtttct ccacatcgat caagagactg tactgaccta tttacgagaa
     6001 gtgattcctg agaccgttta ccagtttatc ggtcctgcaa tcaaagacct tttgacacaa
     6061 agttcaggag gcttgttatc cgtttctgca ttagcggctt tatggtctgc aagtcaaagt
     6121 atcaatgctt tgcaaattgc gatgaataaa gcgtatggcg tagaaaatag aaaaaatttt
     6181 ttgatcgtcc gtttcttctc actaattgtc attgtcttgt tcatgattgc tattagtggg
     6241 gtaactatcg ttcttgggtt gggacagctg attttggatg ctattcagcc aatcgtacaa
     6301 atacccgtga actttatcga tcagttccaa actttgaagt ggccaatcac attggtagct
     6361 ttatttgtca tcatgttctt gatttattta attgtcccta atgctcagtt gaagttgaag
     6421 acgatcattc cgggcacgat cttcgctact gtcggctgga tgctgttatc acaagtgttt
     6481 agtatctatg ctaggtattt tgcaacgagg atcagcggtt atcagatcat aggaagtttt
     6541 attgtactca tgctttggct taattttgct gctacgatca tcattttggg cggaatcatc
     6601 aatgcagttg ttcaagaata cgtgacgggt catcaaatca aaaaacgtcg agatccaagc
     6661 cgaaaatggt tcaataaatt gaaaactaaa ttttctcata aaaaaaagta gaaacaagag
     6721 ttcttattgt aaatttatat cagtaattta taataattat gactcttaat taaagtaggt
     6781 gcccatatgc ttgtttcttt tagtatggtt ataagatttt ttctgacaat tttattatca
     6841 ctgatattaa cacctgtatt caaaatcatt tcagtacaaa caggtatggt cgacaaacca
     6901 aatgaaagaa gaataaataa agttccaatg ccttcagcgg gcggactgcc tatctttatt
     6961 tcatttgtta tatcgacttt attcttattc cgtaaaatta tccctcaatc gtatattgta
     7021 ccgatcttga ttgcttcttt agtaatcata gtcacaggag tgctcgatga taaatatgag
     7081 ctgacaccta aacaaaaaag tatagggatc ttgattgctt ctcttattat ttattttgta
     7141 gcagatatac gtattgattc cttcacgctt ccttttattg gatatatcca actaggctgg
     7201 cttagttttc ccgtaacgat tttttggatc ttcggtatca cgaatgcggt caatctgatt
     7261 gacggtttgg atgggctggc ggcagggatt tcattgatcg gtttaattac gataggaatc
     7321 attggatatt ttttcctcca tgcttctaca gtgtatattc cagttgttat tttctgtctt
     7381 gtagcaagta ttatcggttt ttttccttac aatttttatc cggcaaagat ttatttaggc
     7441 gatacaggag ctctattcct agggtttatg atggccgtcc tttcactaca agggctgaag
     7501 aacgtgactt ttgtttcatc gatttctttg ctgatcgtca tgggagtgcc ggtaacagac
     7561 acgttttttg ctatcattag gagaaaagca aaccgtgttt ctttttctac agcagataaa
     7621 aaacatcttc atcaccgact aatcagttta ggctttactc ataaaggtgc tgtcctgacg
     7681 atttatgcga tggctttgat gttttctttc acagctatgg tcatgaatta tactggaaga
     7741 atcggaacga tcatattgat tattgccatg ttgtttgctg caattttatt atttgaattg
     7801 attggtctga tcaatgagaa acatcagttt attttaaaaa cattgcgatt tttagggaat
     7861 aaagagtacc gtacgcaagt aaaacagaaa tatttgaaga aatggaaccg tcatatagat
     7921 gaataacaaa ttaaaccagc cgatccttat tagtattgtg acggcaaaca gtgataaaat
     7981 ttttcggaca ctagataatt ttatttcaac tgtaaaagat gaaacagcag taaaaatccg
     8041 aatttttgac aacaattcac ttcccgagta caaaaaaaga ctaacagagt ataggaaata
     8101 tccctacatc gatatctatt ttcatgatga aaattccgga tttggttatg gacataatca
     8161 caatctgata gctagcgatt ctcgctatgg cattatttgt aacccagata ttttagtgga
     8221 tgaagagaca gttacagcat tggtcttgtt attgaaagaa catccggaat gtgcgatgtt
     8281 agcacctaag attcttaatg aagatggaac aactcagcac ctcatccgtg agcagctggc
     8341 tgtttttgat tacgttttgc gctttgtgcc tttcaaatgg gtcaaaaaaa agtttgacaa
     8401 acgactggct gtgtttgaat gccgaggatt gcctactgat gctaatagtt atgtacggat
     8461 gatctcaggt agttttatgg tagctgatat tgataaattt aaagagataa aggggtttga
     8521 tgagcgtttc ttcatgtact ttgaagacaa cgacttgtgt ttgagttttg aaaaagcagg
     8581 ttacaagtta ttgtacacac ccttttatgc agttatccat ttttacggga agggtgcgca
     8641 tcgaaatgca aaactgtttc gcgtgttttt gacttctatg atgaaatttt tcaataagtg
     8701 ggggtggcgt tttttctgat gaaaccagaa atattagtag tcgttgtgct ttatcagcaa
     8761 ctattcagtc aatctccagc ttatggacca ttaagcaaag cacttagcga aaagcaaatt
     8821 cagcttatta tttacgataa tagtcctcaa aggcagcaaa atgagctgtt tgaaaaagaa
     8881 aatatcgttt atcatcatga tccgcaaaat cctggtttag cggtggctta taattttgcg
     8941 ttgtctcaag cagatgaagg aacacgttgt ctcgtcactc tggatcagga tacgaggcta
     9001 gtcgacgatt attttgaact attgcgtaaa gtaaccttta cagatgaatg tgttgctgca
     9061 gttccgatga ttttttctgg gagccgacaa gtttctcccc tatattcaga tcgttatatc
     9121 aatcgtacag ctcaagcagt agaagttgaa ggcccaacag ctgaacgaat catggcaatc
     9181 aattcaggtg ttgcctggtc tgtcaaattt ctaaaaaaga ttggcgggtt taatcctgct
     9241 ttttctttag attttttgga tcattggctg ttttggaaag tggatcagtt ggaaaaaact
     9301 gtggagatcc ttcctgtacg aatggaacat gatctttcgg ttttagacta tgaaaaagtt
     9361 tcaagtaatc gataccgttc tattttacaa gcagaaaatc gattttatca tgaatatgat
     9421 caaaggcatt tatccagtca ccgacgacaa ttattgttaa gaacagctaa acagttcttg
     9481 accgtcaaaa accggcatat ctggcggatg actttgcgct catatgttaa gaattggaag
     9541 gtgtgaattc gtgcgttcgg tttgtatagc tacctttaat ggaggcagat atttaagaga
     9601 gcagttagat agtattttat ctcagctttc agcagaagat gaagtagtca tttctgatga
     9661 tgggtcagta gatgatacat gggatatatt aactgaatac gcagaaaaag ataagcgaat
     9721 ccagcttttt tcggggcctc gtcaaggatt gatcgctaat tttggttatg cgatccagca
     9781 gacgaaagga gatctaatct ttttggcaga ccaagatgat gtttggttgg agacaaaagt
     9841 gaaaaagatc actgactatt ttgagcagaa tccggatcag ctagtagtag tttctgattt
     9901 aatcattgtg gacagcgagc tgcaagagat ccatgattcg tatttttcgt ttcgaaattc
     9961 caaagaagga tggcttaata atgtgataag aagtcactat attggtgctg gtatggcttt
    10021 ccggtcttct ttaaaacccg atattttgcc gatcccgtca gaagtgccga tgcatgatat
    10081 gtggattggt ttgctgggag gaaagaagac tgggttccta agagaaccgt taactctgta
    10141 ccgaagacat gatttaaacg cgagtgagat tgaaacacat tctaaattac ggcaaaaaat
    10201 ttcttggcgt gttcatctga ttacggcgtt gatcaaacga aaatggttta aacgttgata
    10261 aaagatgttt aaatccttaa tatttccatg acaaatataa caattgatga gataataaca
    10321 aagtgaaatt tgaaatcaaa aaggagagca gatatccatg aagggaatca ttcttgctgg
    10381 gggcagcgga acacgtctgt accctttaac aaaggcaaca tcgaaacaat tgatgccgat
    10441 ttatgacaaa ccaatgattt attatccaat gtctactctg atgttggcgg gaatcaaaga
    10501 aattctgatt atttcaacgc cacaagatac accacgtttc aaggaattat ttggaaacgg
    10561 aaatgattta ggactgcaga tcgaatatgc agtacaagaa agtccagatg gattggcaca
    10621 agcgtttatc attggagaag aatttattgg agatgatagt gtctgcttgg tattagggga
    10681 caatatttac tatggtggcg gtctgtctaa aatgttgcaa cgagcagctt caaaagatac
    10741 aggcgcgaca gtatttggct atcatgtcaa tgatccagaa cgctttggtg tagtagaatt
    10801 tgatgagaat atgcaagctt tgtcgattga agaaaagcct gctcagccaa aatcaaatta
    10861 cgctgtaact ggcctttatt tttatgacaa tgaggtaatt tctatcgcta aaggtatcaa
    10921 accttcagaa cgtggagaac tagagattac ggatgtcaat aaagcatatt tggataaagg
    10981 aaaactatcc gtagaactta tgggacgcgg atttgcttgg cttgatacag gaacacatga
    11041 atcattgctt gaagcatcta cctttatcga aacaattgaa aaacgtcaga acttaaaagt
    11101 ggcttgtttg gaagaaattg cttaccgtat gggctacatc gataaggaac aattgctggc
    11161 ccttgcacag ccattgaaaa aaaatcaata cggtcaatat ttattgaatt tggccgctga
    11221 ataaggagtc cttacagatg aaagtaacag atacaaaatt aacagatgtc aaaatcgtag
    11281 aaatggacgt ctttggcgat catcgcggtt tcttcacaga aagttattca aaagcaaaat
    11341 ttgcagaaca tggattgaaa tatgactttg ttcaagacaa ccattcacta tcagctgaag
    11401 ctggtgtcct tcgggggctt catttccaaa aaggagaagc tgcacagaca aaattgattc
    11461 gggtagtcac tggagcagtt ttagatgtag tagtggatat ccgcaaaggt agcccgactt
    11521 atggtcaatg ggacggattt atcttatctg aacataatca tcgacagttg cttgtaccta
    11581 aaggatatgc ccatggattc gttactttga caccaaatgt gaacttcctt tataagtgtg
    11641 ataattacta taatgcagaa gcagatgggg gcatcgcatt tgatgatcca gatttagcga
    11701 ttgattggcc aattgatcca tcaagagcga ttatgtcgga aaaagatcac cgtcatccga
    11761 cattaaaaga atttgaagca gaaaatccat ttgtttacgg agaaatctaa gaattaagga
    11821 gaaattttct tatgaaaaac atcattgtta ccggaggtgc cggattcatc ggctctaatt
    11881 tcgttcatta cgtggtaaac aatcatccag aagtgcatgt cactgttttg gataaactga
    11941 cttatgcagg aaacaaagag aacttggccg gacttcctag cgatcgcgta gaattggtcg
    12001 taggagatgt tgcagatgca gaattagtgg accgtctagt aaaagaagcg gacgcagttg
    12061 ttcattacgc ggcagaatcc cataacgata attcattaaa agatcctttc ccatttgtac
    12121 aaacgaatat cattggtacg tatacacttt tagaagcttg ccgcaagtat gatgtacgct
    12181 atcatcacgt atctactgac gaggtttacg gcgatcttcc attacgtgaa gacctgccag
    12241 gacatgggga aggcgaagga gaaaaattca ctgcggaaac accatataat ccttcaagtc
    12301 catattcttc tacaaaagct ggttcagatc tgttagtaaa agcatgggtg cgttcttttg
    12361 gcttacgtgc aaccatttca aactgttcaa acaactatgg tccttatcag catattgaaa
    12421 aatttattcc tcgccaagta acgagtatct taagcggtat tcgtccaaaa ttatatggcg
    12481 aaggaaagaa tgttcgtgac tggatccata cgaatgatca ttcttcagca gtgtggctga
    12541 ttttgacgaa aggcagaatt ggcgaaacct atctgatcgg tgcagacggt gaagaagata
    12601 ataagaccgt gatggaattg atcttagaaa tgatgggcca accaaaagac gcatacgacc
    12661 atgtaaacga ccgcgcaggg cacgatttgc gttatgcaat cgattcgaca aaattgcgag
    12721 aagagctagg ttggcagcct gagtttacga acttccgtga tgggttggct gacacgatca
    12781 aatggtacga agaacatgaa gactggtgga aaaaagaaaa agaggccgtt gaagcagcat
    12841 atgccaaaaa tggacaataa tacagaggaa atttcactgt attaatggaa aaacacatgt
    12901 gaggcaggcg ctcgcctgtc tcttttttaa aataaaaatc aaggagcgga taaccatatg
    12961 tttttattaa ctggaggaaa tggacaatta ggtacagaat tgcgccattt attagatgaa
    13021 aaaaatctgc catatgtttc aacagatgca aaagacttgg atatcacaga tgctgagaaa
    13081 acaatggctt atattacaga attaaaaccc gatgttattt tccattgcgc agcttacaca
    13141 gcagtagata aagcagaaga agaagcaaaa gaattagatg agaaaatcaa tgtcgatggc
    13201 acaagaaatg tagcacttgc agcaaaagct gtgaatgcac aattcgtcta tatcagtaca
    13261 gactatgttt tcgatggaaa aaacaaaaat gtagaatacg aagtagacga ccaaacgaat
    13321 ccactaaatg aatatggtcg tacgaaatta ttaggcgaaa aagcagtaga agagatttta
    13381 gaagactatt atatcatacg aacttcttgg gttttcggga tctatggaca caatttcgtc
    13441 ttcacgatgc aacgcctagc ggaaacccgc gacaaactga ctgtagtaaa tgaccagttt
    13501 gggcgaccaa cttggacacg tactttggca gaatttatgg tatatgtcat cgaagaaaaa
    13561 gcaccatttg gtatttatca tttgtcgaac gaaaatagct gttcttggta tgagttcgca
    13621 aaagaaatat tgaaagatac agatgtagaa gttgcacctg ttacgtcaga agaatttccg
    13681 caaaaagcaa cacgtccgca gtattcagtc atgagcctga aaaagacaga agcgttaggt
    13741 tttgtcatac caacttggca agaagcactt gcgcaaatgc tggaagctgc caaaaaaatg
    13801 aataagtaat ttccttttta tcaaatagaa tttggagcat gatatggaaa aacaaacaag
    13861 aagtgcacga cgaaaagaaa agccaaaagc gaatcaggca gattcgaatt tggtgcttga
    13921 cctgtttcta aaactccgct atcttattgg cgttttagtc atcgtggtca tcgttacttt
    13981 taatttaaac ggaagttcac ttggtgtttg ggataaatat gtgtcgcaaa gagacgatgg
    14041 gaaaaaaact gatgtgattt ttggacagaa tcgggaagtg cggtcagacg aatggctggt
    14101 gcagacacct ttttacctct cacaagctga agaggacttt tcgttggtga acgaggatta
    14161 ttcattaaac ggacaaaaca tgatcattgc gtataattct cctgtaaagg atattacggt
    14221 tatcggaaaa ccattcaact ggggattctt tttcctcgga agagatcgag gactttcctt
    14281 ttattgggcg atgaaattag tcgtcatggt cttgcttgga ttcgaagtac tgatgatatt
    14341 aacgaagagg aaaaaagctc tatcgctaat cggtgcattt gcgctgacat tttctccagc
    14401 agtccagtgg tggttcatgc aacatgtggg cgatttgatt ttctttactt tgggactgat
    14461 gattgctttt tatcattatt tctacaatca tgataaaaaa tggctacgaa cgttgatgat
    14521 gcttttggtg gttattttcg gtattgggtt tattttagtg atttacccag cccatcaagt
    14581 gatgctggcg tacttgctga ttttttattt tgttggcctg cttatttact atagcaagaa
    14641 agtttcatgg gatctgttgg atgtagtgtt gattggaggg gcagttttat ttattggcgg
    14701 agtcatggtt catttctggc taacgtcaaa agacgcattg ttagcttctt tgaatacctt
    14761 atatccagga aaacgggtaa gtactggagg aaagtggacg attggcgaat ttttttcttt
    14821 ccttacaaat tggaaaattc cttttgaaga tatcaactat tcgaataact ctgaagtggc
    14881 cctgttctat cacttttttc caactgtatt cctagcaagt ccatttgtgt tatttggcaa
    14941 aaaagatagc gaacaaaagt tactgggacg tattttgatg attttttgtc tgttttctat
    15001 cttttggatt accgttgggt tgccaaaatg gctagcagaa atcactctgc tgtcttatgt
    15061 tcctccatta agagccatcc ttacttttag ttttgcggct tgtttattat cgatctggtt
    15121 tatcgcttat atttggagag aagatgttat tcctatttgg tataaattca ttttgttggc
    15181 agtaaatgca tggtcttatt tctatgcgat tacgcattcg aaaatggata attatttttc
    15241 ggattttgaa accatcgcag ttgctgtgtt gggaacagtg attttagctt ttgcattatt
    15301 tggctggaga cgcttgtttt acctcgcttt tgtcgcctta gttatcgtat ctggcttttt
    15361 cgtcaatcca gtcgtagaag gtacaggagc gatttttgaa aaaacactgg caaaagaaat
    15421 ccaaaaaatc gatcaacaag atcctggtaa agtttggttg tctgaagatg aattatataa
    15481 cttcacccca acattgggag tgaaatcctt caatacagta cgcttttatc cagatatagc
    15541 tgcatggaag aaaatcgatc cgaaagaaga atacgagaag tattataatc ggtatgctca
    15601 tacaagagct tttatcgcta aggaagatac aaagttcatt ttagatcgtc cagatatgtt
    15661 ttcggtcacg ttgaattttg aagacgcaga aaaattaggg atcgactatg tgatcagtaa
    15721 acgaccatta actgattaca accaattata tgaagcgcag ttcacgcagc tatatggacc
    15781 ggataaagac ggttatatga tttttaaagt agaatatccg gattatccag atgtaccaca
    15841 aacacaaact caggaaaatc agctagtcgt acagtaatag gctagctgag aataatcctg
    15901 aaatattagt agaaaggaga gtgaagataa ttcaagcaat cgtacaaaaa tttgtcaaca
    15961 aaatgagtgt cggtttcttg cttttgttgc tgacgctagt cttttttgtt tggacagcag
    16021 gggtaggttt tccagcaact ttgtttatat ataacaatag ttcattactt atcgctttta
    16081 gtgtggctat tttgctcatt ttaaatgttt ataaagcaaa aggcgtcgat tttctttatc
    16141 taggtgttgc gcttgccctt ttcattttct tttcttactt tcgggaatta agaaatgagt
    16201 ctttatttgg agacttactg tttcctttga ttattttatt ttgtttagta attaaatgga
    16261 tcgagtggac aaaatgggat cgcggtattt atctgattgt ctttacggtc ttcctcggaa
    16321 tcacgttata tcgtgtattt acagagatcc aagtaccaga aggacaaagc atctgggctc
    16381 caggcaattc gctttctgat atttggatca atacgaatac aatcggaagt tctttgatga
    16441 ctcttgcttt attgatcagt ggatttgcca gttcttttga acgatggtat gttcgaatca
    16501 tcggtgggat agcagtcgtt gcagcctttt taggtatttg ggtctgccaa tctagaggtg
    16561 cattagtcgc gttgattgtt tttgcgatat tggatctttt acctaaatcg ttgatgcgtg
    16621 tcattcgtgc gccatttatt gcctacacgg ttacgattct tttagctttg ccgatttctt
    16681 atctggcggc tgtctctgaa aaagtcaatt tgtttaccgg tcgagaagat atttggcata
    16741 aattctatca gacgctagga gaaaaatctg aacaagtttt actaggaatg aaaacattta
    16801 tcttccagcg aggaaaccaa tttttaggaa accacaatag ttataattct attttgaata
    16861 tctatggatt gataggattt gggattgctg ctttgctgct tatccttttt attggtcgct
    16921 taacgttgaa agcagattta tccaacgggc agatgacttt tatctgggcc ttttttgcgg
    16981 tgatgatgca gtcatttatg gaggatacat tgacttcata ccgatgggtt ccgattattt
    17041 atatcctgtt ggctatggcc gttcatcgat atgatgaacc agaagttacg gaggaattct
    17101 atgttatgga agaggcatat gacgatatga cttatgatga tacatcttat gaggaacaac
    17161 cagatagcgc tcattcttcc aggatccaac gctatcattg ataggcacaa aatacgagag
    17221 aggctgtgac aaccgtcgca gccttttttg gtttattgca attgtctagc atcttctgta
    17281 aaatagaaac gttagagaaa atcttgtatc atagaaaatt aagaaagaaa taagaacgca
    17341 gctcggtacc aaaaatatgg taaaatggca cttgactgta attcactgaa aatcgcttgg
    17401 aggaacacaa atgtttaaag atttgtattt atttatgaag gacatttatc agaacagaaa
    17461 attactgcta cagttttcga tcaatgactt caaatcaaga tatgcaggat cgtttttagg
    17521 aatcttatgg gcgtttatca atccgctatt tactgtattg atttattggc tggtatttgg
    17581 tgttggactg aaagcacaaa tgatcgatgc aaaataccca tttatcgtat atttagttac
    17641 cgggatgatt ccctggttct tcttctcaga tgcatttgca tcaactacgg ttgtttttcg
    17701 ggaatatact tatttagtaa agaaagtcgt attcaatatt cgtatcttgc ccacgacgaa
    17761 aatcctatcc aatctgtaca cccatttatt cttcattctg attggcatag ctgtcgcgct
    17821 tataaatggt atatatcctt cagcaatgtc tttacagctg atttattatt tattttgcat
    17881 gtgtgctttc ttgacaggat tgacttggct gacggcatcg atccaaccat ttttacctga
    17941 tatcatgcaa ttaattacga tcgtgctgca gttgatcatg tggacattac cgatattatg
    18001 gagccctaat cagtttgatt caggtcttgt aagcatattg aaattaaatc cgctgtacta
    18061 tgttgttcaa ggttatcgtg agtcattttt aagtgaagct tggttctggg agcatggaac
    18121 atatactttg tatttctggg ttttcacgtt agtaatgttc gtaatcggct ctgcagtgtt
    18181 ccgtagattg aaaccacatt tttcagatgt gctgtaagga gaatagctat gacagaatat
    18241 gcaattagac taaaagatat cacaaaatct tataatatgt acgcaaaacc ttcggatcga
    18301 tttcgtgaag ccctcaatcc ttttaagaaa tcctatcatg atgtatttta tgcgttgaaa
    18361 aacgtgaatg tagaaataaa aaaaggtgag atgatcggtt tcgtgggaga aaacggatcg
    18421 ggaaaatcta caatgttgaa aatcattacg ggagtactga caccaacatc cggcacgatg
    18481 gaaatcgaag gaaaaatctc agctttgcta gaactaggat caggattcaa tcctgagtat
    18541 tctggctatg aaaatatcta tttaaatgga atggttctag gattttcaag agaagaagtc
    18601 gatgaaatgg tcgatgatat cattgaattt gctgacatcg gcgaccatat ttatcaacct
    18661 gtaaaaacct attcaagtgg gatgttcgtt cgtctagctt ttgctgtagc gatcaatgtg
    18721 aatcctgata ttttgattgt ggatgaagca ttggcagtag gagatcttga atttcaacta
    18781 aaatgtatgg aaaaatttac agaaatcaag aactcgggaa agacgatttt attcgtttct
    18841 catgatatta gttcgattcg ccgtttttgt gatcgttcct attggttgaa aaatggaaaa
    18901 gttgtcgaat atggtgatac gatggaagta acgacgaatt acgaaaactt tttgaagaaa
    18961 aaatcagtta aaacagtaga tcgaaataaa aaccaagtag aacgcttcgc agctccagat
    19021 atcgtagacg tgacgaaagc cgaattgttg gataaagata aaaatccagt ggatatgctg
    19081 gaacaaggag aaaaactata cgttaaagta acctatgaag taaaagacga tacaatcaaa
    19141 aatccagttt taggaattgc tctgcgtaca gtcgacaatt tttatgtgtg cggactcaat
    19201 actctactag atggtatcaa gatcccttgg aaaaaaggtg agaatatctt ttatctggag
    19261 tatgaaaagt taggtttgtt agctggtgaa tattattttg acgttgcggt ctttgaagaa
    19321 aatgcaacgg ttcctttggt atacaagaca aaatacatga acttgtttgt cagcggttct
    19381 tatatcggag aaggcatcgt ggtcttagac cataaatggg aagaagggac tcatagcaat
    19441 gaaatatgat tttgaaatgg acctagatga acaaagcagt gtgggaaaaa ttgccgcaca
    19501 gatcaaacca ggaagtaaag ttttagagtt tggtcctgga aacggccgat taacgaaaca
    19561 tttgatcgga gcaaaacaat gtgaagtgag cattgtcgag ctagataagg aattgttcga
    19621 ttttgtcagc gagtttgccc aagatggatt ttatggagat atcgaaagtt ttgaatgggc
    19681 aaactattat gctggacaaa catttgatta cgttttgttt gccgatgttt tagaacattt
    19741 ggtagatcct ggaaaaacgt tgaagaaagt tagagaattt ttgaatgaag aaggagaaat
    19801 tctaattaca ttccctaacc ttgcgcataa ttctgtcatg attgatttgt tcaataatca
    19861 attgccatgg gcttcttatg gtttgctaga tgaaacccat aattcttttt atactcatga
    19921 tggcttccaa aaagtttttg aaaaagcagg attatacatc aatattgaag attatttgta
    19981 cttagctgta ggtgatacag aattaaaatc aacttatgaa gaattgccgg aagctgtacg
    20041 ttatgatttc aaaatgcgac catttggcga agtctaccaa tacttcttct cattgatgaa
    20101 acatccagta gcacaaagtt ccattgcgca gccgcaaaat tctaattatg tgaaagtatt
    20161 agaagtaatc cagcaaacga aacaagatga aataattcaa caaataccat tcaataattt
    20221 cacaggggaa aatgagacac taagtttcca tgtatcagaa ttagcagaac gaatggtgtt
    20281 tcgcttcgcg cagcaaccta gctttatcga attcagcgca gaagctgctg gagaaaaact
    20341 cacatttatc gattcaaatg ctgtagtcaa aacagtaaat gattgttatt tgtttgatgg
    20401 taaagattta ccagagttct ttttaacgga tattgcaggg aaagaagtga ctatccattg
    20461 tcactatcgt tttatcggag aattgacacc aacaatgaaa gaattgctgg aaacaatccg
    20521 tccaatggct gaagttacaa agcagctttc tgagaaaaat gatgaattag aaagagaaaa
    20581 tcattatctt attgagaata atacaagatt agatcagacg ttgaaaacaa ccataaatcg
    20641 ctattgcacg ttgttggaaa gtgatgaatg gacaatcaaa catagattag ggcgacgtaa
    20701 gaaagaaaca tcgaaaaaaa tccaagaaaa agagctttct ctatgtattg acgaaaaaat
    20761 ctgggatgca gaaacaaaga tattgaaaat catcgggtgg ggaatcgcta attcggatcg
    20821 ccaaccattg tcctataagt tatcagccga ccaatcacca ttttttgaag caattcctgt
    20881 tttacgagaa gaagtcaatc aagcaaaaca gctagctgaa ggagcaaaag caggatttga
    20941 actgcggatc ctttgcgaga aggaacgttc attcttagtt gaagcagtgg ctcaaaatgg
    21001 tcaatcatgg atcattgaaa tgtaaataat gattaactta taaagataag gagtaaacga
    21061 atcgtgcaaa aaaaagaaat tgccgtattt atcgatcaaa tcactcgtga acgggccact
    21121 ggcgatttgc ttattgtggg ctgggcaatt gatgaagtga ccaaagaaat ccctacgata
    21181 aaggtagaaa aagaaaatgt gattgctgaa gcaacacatg tcgtaagatt ggatatcaat
    21241 catttatata atcttgatgt taagacccaa agcggtttta aggtacgttt atcagggaaa
    21301 atgagaggaa aagcaatctt agacttccaa acagcaagac atcaaaatgg catagctgtg
    21361 aaattaaacg gtaaatatcc ttatgatgac ggcattgaat cttcatggga aagaaaaaaa
    21421 agattactga aaaaagggat aaattatgca aggactcatg gcgtaaaaaa agcaatccgc
    21481 cgggtcaaac tggaattgaa accaggatcg atcgactatg cagagtggat cagtcgtcat
    21541 gagaaacctg atctgaaaga gcaaaggaaa gaagtacaag gatttgccta ccgcccattg
    21601 atttccatac ttatgcctgt ctataacgtt gaaatcaaat ggctcgaaaa atgcattgat
    21661 tcggtacttg atcaaacgta tgatcactgg gaactatgta tcagtgatga tgcttctact
    21721 gatccagcta tccgaaagtg tctagaatcc tatcaagcaa aagatgatag aattaaagtc
    21781 gttttccgac aagaaaatgg acacatcagt ctggcaacta attctgcatt ggaaatggca
    21841 gaaggagagt ttatagcgct cttagataat gatgacgaat tacctccatt tgccttatat
    21901 gaagtggcaa aagtgctaaa cgtacatcca gaactggatt tgatctacag tgacgaagat
    21961 aaaatcgatg cagatgggaa tcgctttgac ccgcacttca aagcagactg gtctcccgat
    22021 acattgatgg gaaataatta tatctctcac ttaggcgtat atcggacaag cattgttaag
    22081 gaacttggcg gtttccgtaa aggatatgaa ggctctcaag attatgattt ggttcttcgt
    22141 gtgacagaac aaattcctgc agaacacatt taccacattg accgtgtctt gtatcattgg
    22201 cggacgatcc cgggatcaac tgcaagcaat ggagaagcta aaagctacat ctatgattct
    22261 ggagtgaaag cattgacaga tgctctatct agaagaaata tcaagggaag cgtacatcct
    22321 ggcagaattt ctggttttta tgaaatcact tatgatgttt tgcaagaaga cttggtcagt
    22381 gtgatcattc caactaagaa tggctatgaa gatctaaaaa cttgtgtgga ttcgatcatc
    22441 gaaaaaacgt cttatccaaa ttatgaaatc attattgcag ataatggaag tacagatcct
    22501 aaaatgcaag aattatttgc agaatacaag catcagctga aagaccgatt tatcgtcgaa
    22561 ttaattgaca ttccattcaa ttattcacgt atcaacaatc tggcagcaga aaaagcaaac
    22621 ggcaaatact ttttattctt gaataacgat acagaagtga ttgaacctga ctggatgaca
    22681 gcgatggttt cttatgcaca gtttgatcga atcggctgtg taggagcaaa attattttat
    22741 ccggatgata caacgcagca tgcaggtgta ttattaggga tcggtggcgt tgcaggacat
    22801 gcattgaata actatgatcg aacacattgt ggttactttg gccgattggt cattgacgtc
    22861 aattacttag cagtcactgc agcttgtatg atggtcaagg cagctgactt caatgctgtc
    22921 aatggatttg atgaaatatt agaagtagca ttcaacgatg ttgacttgtg cttgaaagtt
    22981 tatgaactgg gtcgttacaa tgtctatgca catcaagcag aattgtacca tttcgaatct
    23041 aaatcaagag gttatgaaga tacaccagaa aaacaaagac gttttgctgg ggaaatcaaa
    23101 aaaatgcaag ataagtggcc ttcttatatt gctcatgacc cattttataa cgataatcta
    23161 actaaagaag gaatcggcga tttttcgttg agacctgatt gattaaaaga actgtatttt
    23221 catttttatt aatgaaaata cagttctttt aatttaatt
//
LOCUS       DAZUKC010000042        22973 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000042 DAZUKC010000000
VERSION     DAZUKC010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.043"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            40..522
                     /locus_tag="ACG80T_001570"
     CDS             40..522
                     /locus_tag="ACG80T_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841216.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     LVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            719..1609
                     /locus_tag="ACG80T_001571"
     CDS             719..1609
                     /locus_tag="ACG80T_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFS4841217.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            1935..4409
                     /locus_tag="ACG80T_001572"
     CDS             1935..4409
                     /locus_tag="ACG80T_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS4841218.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLLKRRNNTVAMTGDGVNDI
                     LAMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLV
                     KNIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNV
                     MEKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKF
                     RSQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLF
                     ERKNEKEQKPFSRVVKKFLSIFKREN"
     gene            complement(4512..6248)
                     /locus_tag="ACG80T_001573"
     CDS             complement(4512..6248)
                     /locus_tag="ACG80T_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4841219.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNGDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRAYTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLHGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(6245..6949)
                     /locus_tag="ACG80T_001574"
     CDS             complement(6245..6949)
                     /locus_tag="ACG80T_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4841220.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            7080..7585
                     /locus_tag="ACG80T_001575"
                     /pseudo
     CDS             7080..7585
                     /locus_tag="ACG80T_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
     gene            7545..7883
                     /locus_tag="ACG80T_001576"
     CDS             7545..7883
                     /locus_tag="ACG80T_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFS4841221.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            7904..9322
                     /gene="ffh"
                     /locus_tag="ACG80T_001577"
     CDS             7904..9322
                     /gene="ffh"
                     /locus_tag="ACG80T_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFS4841222.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDTVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            9434..10012
                     /locus_tag="ACG80T_001578"
     CDS             9434..10012
                     /locus_tag="ACG80T_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFS4841223.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            10016..10537
                     /locus_tag="ACG80T_001579"
     CDS             10016..10537
                     /locus_tag="ACG80T_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFS4841224.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(10616..11170)
                     /locus_tag="ACG80T_001580"
     CDS             complement(10616..11170)
                     /locus_tag="ACG80T_001580"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFS4841225.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            11423..11698
                     /gene="rpsP"
                     /locus_tag="ACG80T_001581"
     CDS             11423..11698
                     /gene="rpsP"
                     /locus_tag="ACG80T_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFS4841226.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            11710..11961
                     /locus_tag="ACG80T_001582"
     CDS             11710..11961
                     /locus_tag="ACG80T_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFS4841227.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            12085..12876
                     /locus_tag="ACG80T_001583"
     CDS             12085..12876
                     /locus_tag="ACG80T_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841228.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTATTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            13046..13567
                     /gene="rimM"
                     /locus_tag="ACG80T_001584"
     CDS             13046..13567
                     /gene="rimM"
                     /locus_tag="ACG80T_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFS4841229.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            13557..14318
                     /gene="trmD"
                     /locus_tag="ACG80T_001585"
     CDS             13557..14318
                     /gene="trmD"
                     /locus_tag="ACG80T_001585"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFS4841230.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            14454..14801
                     /gene="rplS"
                     /locus_tag="ACG80T_001586"
     CDS             14454..14801
                     /gene="rplS"
                     /locus_tag="ACG80T_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFS4841231.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            15111..15353
                     /locus_tag="ACG80T_001587"
     CDS             15111..15353
                     /locus_tag="ACG80T_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFS4841232.1"
                     /translation="MNDGKVKQVPSSTKKKNILLKEVLKRFDHGVTYTETEVNSILLN
                     VFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(15441..15587)
                     /locus_tag="ACG80T_001588"
                     /pseudo
     CDS             complement(15441..15587)
                     /locus_tag="ACG80T_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            15644..15958
                     /locus_tag="ACG80T_001589"
                     /pseudo
     CDS             15644..15958
                     /locus_tag="ACG80T_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345994.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
     gene            15987..17246
                     /locus_tag="ACG80T_001590"
     CDS             15987..17246
                     /locus_tag="ACG80T_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4841233.1"
                     /translation="MKRWKKMMIGSIVLGMSLVFGACGNQEDSSEESANEITVSTWNY
                     ETTPEFKALFEAFEKKTGIKVKAVDIASDDYDTKLTTMLSSGDTTDVLTMKNLLSYSN
                     YALRDQLVDQTERIRELDTDAAEETYEMYDIDGKTYALPYRTDFWVLYYNKKMFDDAG
                     IEYPKNLTWDEYEDLAKKLSKNDGQVYGAYQHIWRSTIQAIAAAQNEANLVEPDYQYM
                     TDYYDRALRMQKEGAQMDFGTAKSTKVTYQSQFEEQKAAMMYMGTWYMAGILANKEAS
                     KTEVEWGITAIPQKKKGESVTTFGSPTAFAVNKNSKKQKAAQEFIEFAASEEGAKVLA
                     GVGVVPSYRTDEIDQLYFNLAGMPTDEISKKAFSPDEIKLEFPIDTYGPAIDKILQEE
                     YDLVLVGDETPEKGTVNMEKRVAAEKE"
     gene            17299..18195
                     /locus_tag="ACG80T_001591"
     CDS             17299..18195
                     /locus_tag="ACG80T_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4841234.1"
                     /translation="MENSINKKATNHLRRKRTWIAYSFIAPNFIGFFVFTLIPILFSL
                     VLAFMKWDSFSTPEFAGLDNFRKMANDETFWISLKNTFLYTAAVVPGTLVCSLGLALL
                     LNQKIRGMKLFRTIFFFPYVTSLVAIAVVWNMLFHPTVGPINIFLRRFIEHPPGWTSS
                     SNWALWAIVIVSIWRGMGYYMILYLAGLQSIQKELYEASSLDGANRWKQFLNVTLPSL
                     RPTTFFVTIMLVINCFKVFDLVQVMTDGGPGRATNVLVYQIYNEAFKKFDFGYASAIS
                     LVLFVIVLGVTVVQFSWNKRRT"
     gene            18206..19075
                     /locus_tag="ACG80T_001592"
     CDS             18206..19075
                     /locus_tag="ACG80T_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4841235.1"
                     /translation="MEESVRNERKKAKIRSKNMPFIQWLGMILLIVLALLTIIPFLWM
                     VSASFKANNEVLTIPIQWIPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFT
                     SSFAVYGFSELNFKGRDILFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQ
                     AFNAFGVFLLKQYYSSIPDSLCESARIDGLSEWEIYRKIILPLTKPAIASLTIITFVN
                     TWNDYMGPFIYLSSTENKTIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQK
                     YFVEGIASSGMKE"
     gene            19085..19760
                     /locus_tag="ACG80T_001593"
                     /pseudo
     CDS             19085..19760
                     /locus_tag="ACG80T_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
     gene            19966..21630
                     /gene="alsS"
                     /locus_tag="ACG80T_001594"
     CDS             19966..21630
                     /gene="alsS"
                     /locus_tag="ACG80T_001594"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFS4841236.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            21643..22353
                     /gene="budA"
                     /locus_tag="ACG80T_001595"
     CDS             21643..22353
                     /gene="budA"
                     /locus_tag="ACG80T_001595"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFS4841237.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            22483..>22973
                     /locus_tag="ACG80T_001596"
     CDS             22483..>22973
                     /locus_tag="ACG80T_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFS4841238.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFI"
BASE COUNT         7555 a         3976 c         4808 g         6634 t
ORIGIN      
        1 atagtatgat gagagaaaaa gtagtttggg ggaacgttaa tgagaagaaa agcagtaatc
       61 gatcggcttt atttattttt ttggtttttt cctatcgttt tgctaccttt tacatgggta
      121 aatgctgttg tacaaggtgc ggagatatta ggcctgatcc aaggaagcgg attagtggtt
      181 tctaggaatt ttccagcttt gactgttttc tatttagtga taagtgtagg tctcttatta
      241 ccaaaatctc tgatcaatca ctacaattgg cagctgcttt acttttcttg cttattagta
      301 tttacatcta tgttttcttt gctgcctcgc ttgattatcg gccctaatgg tttgagcgag
      361 agtggattga aggattcagc ttattcctcc atgtatttct tgacgatcaa accatggttc
      421 tatttagtaa ttgcctcatt tattcttgca acattttttt acttctggac agaaatcagg
      481 ataaaaagtg aacgatctgt atctataaaa aataactatt gatggtggat catcagtaaa
      541 aaagcatatg agctgcgtaa aatgcatgtt tctcatatgc tttttttatt attatgtaaa
      601 gaatgcgaaa aacgtttaca tgatttttac ataacttaac tatattttta cagcaaccat
      661 acgagggatt tacagtcgac tgataaactt tagttgtaaa taagaaagga gcaataaaat
      721 gaaaaaagta ttgtttactc taggtatgat gggaattctt ttagccggat gcggctcagg
      781 atcaggaaat acagatggct ctgccacaca aaataattca agtgactcaa atgaacaagt
      841 aaaaatcgtt gcggtaggtt cgacagcatt acagccgtta gtcgatgcag cacaagaaag
      901 ctttgtacaa gaaaatccaa attatcaaat ctctgtacaa ggaggaggaa gcggaactgg
      961 tttgagccaa gtcgaagctg gagcagttac tatcggaaac tctgatgtat ttgcagaaga
     1021 aagagacgga gttgatgctt caaaattagt ggatcataaa gtagcagtgg tcgggatggc
     1081 accaatcgtc aataaagata cagatgtcaa agatatcacc aaacaggaat tgattgatat
     1141 ctttacagga aaaatcacta actggaagga agttggcggg aaagatcaaa aaatcaacgt
     1201 cgtgaaccgt gcaaacggca gcggtacccg cgcgacattc gaaaaatggg gactggatgg
     1261 tgctacccct gtgcagtccc aagaacaaga ttcatcagga acagttcggc agctggtaag
     1321 tcaaacacca ggagcaatca gctatttagc tttctcatat ctcgatgatt ccacacaagc
     1381 actaagtatc gacggtgtag aaccaaaaga agagaatgta gcagataaca gctggggaat
     1441 ctggtcatat gaacatatgt acacgaacgg aaaaccttcc cctgaagtcc aaaaattctt
     1501 agactacatg atgacagaag aaatccaaga aggacctgtc aaagaattgg gctatttacc
     1561 aatcacaatg atggaagtag aacgtgatca cgaaggaaat atcaaataac tagcagataa
     1621 acatgccacg gtagtcgtta ctattaagga aaaatcaact atgtttcccc acatatacat
     1681 ttttccttta attgtgaaaa atagttcctc atattttgcc gttttttgag ctaataccga
     1741 gggtcagctg attgaacacc gtttattcga aaaaaagagc tacgacatag aactgtcgta
     1801 gctttttttt tgctatatga caggaatgaa aggtgtttgc aagcttaatg tgaggaacac
     1861 tttccatatc tgttctatac tggataggag aagtttggaa atatccagaa aacgaaatca
     1921 atcgaggtag ccacatgaag aataaatgga taaatcgaag taaaaaaaag aaagtaacac
     1981 aaagatcatc agataaagtc atccgtcttt ttccatcgta taaagaagga ttaacggcag
     2041 atcaagtcaa ggaacgaata gaaaaaggtg cagcaaataa ttcagtcgat cccacattca
     2101 aaacaaatca gcaaatcgta ttggaaaaca tatttacgta tttcaatttg atctttctta
     2161 tcttagccat tttgctttgt ttagtagaat cctataagaa cttgactttt ttaccagtta
     2221 tcatagccaa tacaggaatc gccatttttc aggaaattcg atccaaaaag atcttggacg
     2281 aactaaatgt tttaaataca gcaaaagtca atgtcatacg agacgggata gaacaaacaa
     2341 tagatataga agaccttgta atagatgaca ttgttatttt agagactgga catcaaattc
     2401 cagcagatgc agaggtcgtg gaagggaaac tgcaagttaa tgaggcttta ttgacaggcg
     2461 aagcagatga aataaccaaa gaaataggcg atccattaat gtccggcagt ttcattgttt
     2521 ccggaaaagc ttacgcccga ttagacaaag ttggagcaga ttcgtatatt tcccagctga
     2581 cagtcaaagc caagacaatg ggagaaggag aacagtcgga gatgattgct tctcttaatc
     2641 aattgatcaa atggattggg atcatcatta ttcctatcgg tatcctgctt ttttctcaaa
     2701 gttacttttt taatggaaac acgatcaaag aaagtgtcgt tgcaatggaa gcagcattga
     2761 tcggtatgat acctgaagga ctgtacttgc tgacgacgat tgcattagca ttaagtgcga
     2821 ctaaactagc aaagcaaaga gttcttttac acaatatgaa aagcattgag acattggcaa
     2881 gggtaaatgt cctttgtgta gataaaacag gaacgatcac agaaaataaa atgtctgttc
     2941 aaaaagtgat cgtctctaaa aaacaagaaa taatcagtca gtcagatcaa ttggaagcat
     3001 gcattgctga ctatgcaaaa gcgatggcag gagacaatgc aacaatggaa gctgttaaag
     3061 catattttac aaaaaccact gatcaatcct atcaccatgt tattcctttt tcttctgtcc
     3121 aaaaattcag cagtgtagcg ttggcagaca aggtgtatgt tttaggagcg cccgaaatgg
     3181 ttttgcgcga tcaattgccg gaatatgcgg aagagtttgt ttctttcgct gaaaaaggct
     3241 atcgcgtgtt ggtctttggt gtgtacgatg ggattcttga agaaccagta ttgacagaag
     3301 cagtcacacc attaggctat attctgattg ctaatcctat cagaaaagaa gcaagagcca
     3361 catttgatta cttcaaaaaa cagcaagtag ccatcaaagt gatttctgga gataatccgc
     3421 tcaccgtatc caatgtagct gtccaagcag ggatttcaca tgctgatcag tacgttgatg
     3481 catctaaatt atcagaagaa gaatatgaag cagctgtcga aaaatataca gtttttggac
     3541 gcgtcaagcc agaacaaaag aaaatcttcg ttcaattgct aaagcgtcgt aacaatacag
     3601 tagctatgac aggtgatggc gtgaatgata ttctagccat gaaagaagca gactgcagta
     3661 tagcgatggc ttccggaaat gaagccgcca tgcaggcctc acaagtggtc ttacttgatt
     3721 ctgacttttc acgcatgcca gaagttgttg cagaaggacg gcgcgttgtg aacaatattg
     3781 agcgatcagc cagcttgttt ttagtgaaga atattttctc ctttttgctc tcactgtttt
     3841 cagtgatctt tgctctgacc tatcctttag aaccatcaca aatcacattg attagtttgt
     3901 ttactatcgg acttccatct ttcttgcttg ctctagaaga aaacaaaaag cgaatcagag
     3961 ggaaattcat catgaatgtg atggaaaaag ctgtgccagg tggactaaca gatatgatcg
     4021 tagtcggtgc gttggtgatc tgcggggtga ctttgaatct aaataaaaca gatgtttcta
     4081 cagcatctac catgcttttg attgcagtag ggttcctcgt tctctataaa atctgttcac
     4141 cgctgaataa attcagaagt cagatcattc ttttctgtgc tagcggtaac tttttttcgg
     4201 tcatcttttt gcataagctg ttttcaatca ccggtatctc agctgtatca cttttgctgt
     4261 tgagcattct atttttctcg gcagattcga tttttaggca tttgaccaca ttggtagaat
     4321 atttgtttga acgtaaaaat gaaaaagagc aaaagccgtt tagtcgcgta gttaaaaaat
     4381 ttctatcgat atttaaacga gaaaattgac gaaaagtatc tcaaaaaaga gtaaaaaaga
     4441 acaaccactc gtgtatcttt cgaagcagga tgagtcatcc aactttaaaa cgcacgagca
     4501 gttgctcttt tttacatttt cggaatggta acggtgaaag tcgaacctgt tccaagatga
     4561 ctgtctacag taatcgtacc atgaaggagt tgcacgtagt cttttacaat cgctaaccct
     4621 aatccggtcc cgcctgaatg ccggcttcgc gccttgtcta cgcggtaaaa acgctcaaag
     4681 atccgttcct gatcctcaat gtcgatacct ataccaaagt cttggacaga aaacgataga
     4741 tcatctgttg cctgatagcg gataataatc tcgctgtcgc tttttgaata ttggacagca
     4801 ttttcaatca gatttttgat aattggataa aggatatctg ttttagtagt aaatggttcg
     4861 ttctttccaa tcaatcgaat cgtcaaccgt ttcttttcga tgattggctg ataattttga
     4921 acgatttgct ggaaataagg ttctgtataa attgtctgga tctcgtaaga attctcgcca
     4981 tcttttgaca gctggatgat ttcccgaatc agtttatcta accggatcgc atctttttgc
     5041 atgatctcaa gaaaagaagt cagagtttgc gggtcatctt ttgcaccatc cagtaaagtc
     5101 tcggtaaaac caatcagtga agtcacagga gtttttaatt cgtgagagac gttgccaacg
     5161 aaatcctttt gtagcttttc taaacgtcgc actttcgtta agtcatacgc cacccctaga
     5221 atctgtccgg taccttctga attagtaaag tagcgcaaac tcaagtctaa tgtctgccga
     5281 ccttctgtaa gggtgatctc ctgatggatc agcggatgct caggagtcac ttgatgaatc
     5341 agctgaatga gttttggctc ttgaatgact tctgtgtacc gctgttcagg ttgatagctt
     5401 accacgttta aatgtagctg catctttgga ttaagcagta gtaaacgaga atcagcatca
     5461 tcaatcagga atacgccaat catcagttcg ttcagtaacg tatataattg ctcttctgtg
     5521 gtagtatacg cccgatacat cttactcatt tgttcgctca acgcattgat cgtctgatac
     5581 aattcttccc attgatcggg tgtttgcatg attacttccg cctgattagg attttgcacc
     5641 atttttttta gtacgggcag cacggttttc aatggctggt ttttttgata gatcagataa
     5701 tagatcattc ctgcaagaac aaggaagaaa gacagaaaga agaaaaagac ccaacggcga
     5761 aaactctcag ttctcggtaa aaaaccgctt gtcggttctg cgatacgtaa aatggcttct
     5821 aactggccac tttttttgac tggtaatgcg acatataaaa gatccccatt caaagtagta
     5881 cttttgcgca aagcgctacc taggttccca ccattcaata cggctttgat ttcgggacgg
     5941 gagtttcgct gttcattcaa tgtttcattg tttgtgtcaa ataagatctt tccagtagca
     6001 tccattaaag taatccgttc gttcgactga tggacgaatt cttccaaagc agttttgttt
     6061 tgcgctgctt ggaggttttc gacatctaat tgccggatca gcagttgtgc tttttttgtc
     6121 aggtagcttt cttgctgttc cacgatctgc tgttgaaaat aatgggaaat caattgccag
     6181 ccaccaaaaa acaaactaac taaaagaata agaaagaaga gacgttcata aatttttttc
     6241 catttcatcg tttgggttcc tgaaacttat agccaaatcc tctaatagtc accaagtact
     6301 ttgggtgttt tggatctcgc tcgatttttt ctcgcaaatg actaacgtgt acatctacga
     6361 ttcggctttg tcctgcgaag tcaaaattcc aaatgcggtc tagcaatgtg tctcggtcga
     6421 tcacacggtc ttttcgtttc atgaaataaa caagtaattc aaattctttc ggtgttagtt
     6481 caatcagatg gtcgtctacg gtcacttgat aattggtcaa atcagcagtg atttgtccaa
     6541 tcgatagata ttcaggctca gcttcatctt ttgattgctt acgaggttca attcgtctga
     6601 atattgcttt catacgggct aatacttcgc gaggactaaa tggtttggtc aaatagtcgt
     6661 cagcacctat ttctaggcca atgatccgat ccacttggtc atctttagcc gtcagcatca
     6721 aaatcggtgt ttcatttttt tcttgacgca gccgctgagt gatctccatg ccatccatcg
     6781 ttggaagcat cacatctaaa atgatgaaat caaatgattg ttctaatgct aattctaatc
     6841 cttcgcctcc atctgtggca ctggttacct tgtagccttc tttttccaaa ttgaacgtca
     6901 agagcgttac aatagaaggt tcatcatcaa caactagtat ttttttcatt caaatctcct
     6961 ttaaataggt ttcattctgt ttatatttta gcatagaatc agtacaaagg gctattttac
     7021 aaaagaaata aatggtataa taggaaggca ctttgctgaa tagaaaggaa gagcagtcaa
     7081 tgattggaat cagtgcatgt ttaggcggtc tggcctgtcg ctatgatgga cagaaaaaag
     7141 aagtaccaga attgatggaa ctagttgaaa ctggagaagc tgtcatggtc tgtccagaag
     7201 tgatcggcgg attaccaatt cctagagatc ccgctgaaat cattggtgga aatggattcg
     7261 atgtgtggaa agatcaagca aaagtgtatg gacgacaagc ggcgaagatg taacggaagc
     7321 gtacaaaaat ggcgcgatca aagcctatca aaaattgcag gaaaaacaca ttgatctagt
     7381 catcatgaag gaaaaaagcc cttcatgcgg aacccattcg atttatgacg ggagtttttc
     7441 cggaataaaa aaaaatggtg ccggagtggc tactgcttat tttattcagc aaaaaatgac
     7501 tgtgctctct gatgaagaat ggctgaaaac tagaggtgag cagaatggaa atcgaaaaga
     7561 ccaatcgaat gaacgcgctc tttgaatttt attcgacctt attgacagaa aaacaaatga
     7621 actacatgga attatattat gcagatgatt tttcattagg tgagatcgcg gaagaatacg
     7681 aagtaagccg acaagcagta tatgacaata tcaagcggac aagcaagatc ttggaggatt
     7741 atgaaaaaaa acttcatctt ttttctaatt atattgttcg agaacaggta ttggaaaata
     7801 tgactgcata tattacagat aagtacccgg aagataaaaa actacttgaa tatgtacagc
     7861 aaattcaagc aatcgaagaa taaataaaag gaatgaagaa aatatggcat ttgaaagctt
     7921 aactgaacgt ctccagcagg cgatgggaaa aataagaaaa aaaggaaaag tatcagaagc
     7981 tgacgtaaaa gaaatgatgc gggaaatccg ccttgcctta ctagaagccg atgttaactt
     8041 gcaagtagtc aaagatttta ctaagcgcgt acgcgaacgt gcggtagggg cagaagtgct
     8101 ggaaagttta tcacctgcac aacaaatcgt aaaaatcgtt gatgaagaat tgacgatcac
     8161 acttggttct gaaacagcag agttaaataa atcaccaaaa ataccaactg ttatcatgat
     8221 ggctggtctt caaggggcag gtaaaacgac ctttactggt aaattagcca attatttaaa
     8281 gaaaaatgaa aatgctcgtc cgctcctaat cgcaggggac gtatatcgac cagctgcgat
     8341 cgaccaattg aaagtattag gtcaacagct agacgttcct gtatttgata tgggaactga
     8401 agtcagcccg gtagagatcg tccgtcaagg gatggagtta gcaaaagaaa agaaaaacga
     8461 ctatgtattg atcgatacgg caggacgtct gcatatcgat gaaacattga tggatgagtt
     8521 gaaacaaatc aaagaattga cgcaaccaaa tgagattttg cttgttgtcg atgccatgac
     8581 tggtcaagat accgtcaacg tagctgatag cttcaaccag caattaggta ttacaggggt
     8641 cgtcatcact aaacttgatg gggatactcg aggcggggca gcactatcga tccgctcagt
     8701 gacaggcgca ccaatcaaat tcatcggttc tggtgaaaaa ttgactgact tagaagtatt
     8761 ccacccagat cgtatggcta gccgtatcct tggtatgggg gatatgctga cattgattga
     8821 aaaagcgcag caagactatg acgagaaaaa agcggaagaa cttgcacaga aaatgcggga
     8881 aaattcattc gatttcaatg actttattga acagcttgat caagtgatgg gtatgggccc
     8941 actggaagat ctaatcaaaa tgatcccagg aatgaatcaa gtccctggaa tcgaaaacgt
     9001 caaggtagat ccaaaagatg ttgaacgcaa aaaagcgatg gtttattcta tgacgccagc
     9061 agaaagagca aatcctgatc tgttgaaccc aagccgtcgt cgccgaattg ctgccggttc
     9121 tggaaacagt gttgttgaag tgaatcgaat gatcaaacaa ttcaaggaat caagaaaaat
     9181 gatgcagcaa atgtccaaag gggatatgaa catcccaggt atggaccaaa tgtttggttc
     9241 aggcgtcaaa ggaaaactag gcaaaatggc gatgaatcga atgatcaaga aaaataagaa
     9301 gaaaaagaaa aaacggaaat aagatgaaaa aaacagcttc ggtacagact gaaaatgtct
     9361 actgccggag cttttctatc agcaattttt attctttact aatacaatta ctctaaacga
     9421 agaggtggca cagatgagat gtccgtggag tgaacaaaca gaaagtatgc ggcaatatca
     9481 tgatactgtc tggggtgttc ctgaatatga tgaccaaaag ctgttccgaa aattgatgct
     9541 tgatattaat caagccggat tgagctggca gacgatttta aataaaagtg aagcattcga
     9601 tgaagcatat gatcgattcg atattaaaaa agtcgcaaat tatggagaaa caaaaatcag
     9661 tgaactaatg gaaaacaaag gaattattcg taaccgaaga aaaatcgaag cagccgtcca
     9721 aaacgccaaa gcagtcttga aaattcaaga agaaatcggc agtttttcag agtatctttg
     9781 gtcgttttct aatggaaaaa tcgttgattc ttcttatcat tcacaagaag aagtcccaac
     9841 aacaagtcct ctttcagatg aattagcaaa agatctaaag aaaagaggat ttaaatttat
     9901 cggaagcaca acaatctatg cttttttaga agctgtcggg atcatcaatg atcaccttga
     9961 cagttgtttc cgcaaagaag aggtcgtacg tatcggcaga aagggcgaat aaagcatgcg
    10021 aaaaatcata ttttatggtg ctataagttt agatggctat ttggcaacga aaacggatga
    10081 tcttcaatgg ctttttgaca ctccaacagg taagcaaacc acttatgaag ctttttatga
    10141 aacaatcgat acgaccatca tgggcagaaa aacttatcag gaagcaaaaa aatatttgga
    10201 gaccgaagca atttatccag agaaaaagaa tttcgttttt tccacccacg cacaattaca
    10261 attgccagat gctgaggtcg taaacgaaga tcctgttgct tttctaaaaa atctacaaat
    10321 gacagaagga aaagatatct gggttgtggg tggcggtcga ttgatcaaac cgctattgga
    10381 acatcaaatg attgatgaat ggtatatcca gattacccct gtattattag gggacgggat
    10441 tcctttattc caaaaaggaa catatgaagc gcgttttgaa atgctagaca cgacacgatt
    10501 cggtgaattt atcgaattgc attatgtacg aaagtagaag taaataaaat aaaaaaggca
    10561 actatatgca gaaatgtcct gtacatagtt gcctttttta ttaccgttat gctttttact
    10621 ctgttaattt gttagaaaaa tctagatacg catctaaaat cgtttggaag aaacttaatg
    10681 ttccttcaac gatattacca ttttcatcaa ttagattcgc aacgtttccg atatatgctt
    10741 ccggttgctg caaggttggc acattcaaaa agacaagtga ttgtcgtaga tggtggtttg
    10801 cgccaaagcc gccgatccca cctggtgatg cagtaacgac taaacctggt ttatgatccc
    10861 aaacgctgtg tccataaggt cttgagccta catctaaagc atttttcaat actgctggca
    10921 cagaacggtt gtattcagga gtaacgaaaa cgacaccgtc tagtcccctc acttcctcac
    10981 gaaaacgagt ccattcagca ggagcctgtt ctggagtttc gagatcctca ttgtaaaatg
    11041 gcagatcatc gatttttaca aatacacttt cgtacccttc aggaagcaat ttcgcaaaag
    11101 cttctgctac ttttttgttg taagaatctt ttcttaaact accaataaaa aatccaattt
    11161 tttttgtcat acgatttttc ctccttcaaa ctatctataa caatcttaca aaaaataagt
    11221 aactggagca aataaaaaca cttactgaca aaagctacat agaagataga ttacttattt
    11281 ttaacaaaaa agaagtctaa aatcttctat ttcctaaaaa aaatcgaatt taatcaagct
    11341 gtaaagaaaa aaagctttac agggtaaaaa aaaactgtta cactacatct tgtgaataaa
    11401 acaatggagg tggaaataaa taatggcagt taaaatccgt ttaaaacgta tgggttctaa
    11461 aaagagtccg ttttaccgta tcgtcgtagc tgattcacgt tctcctcgtg atggacgttt
    11521 catcgaaact gtaggtactt acaatccttt gaaagaccct gcagaagtag ttttaaaaga
    11581 agacttagtt cttgactggt tatcaaaagg tgcacaacct tctgacactg tacgtaacat
    11641 cctttcaaaa gaaggcgtta tgcaaaaaca ccacgaagct aaattctcaa agaaataagg
    11701 tgaccggata tgaaagattt aagcgactta gtcttaacaa tcgttcgtcc gctagtcact
    11761 tatcctgatc aagtcaagtt agacgtcgta gagtcgaaag acttttacga gtacaatcta
    11821 acagtggctc ctgaagatat tggccgtatc attggaaaac aagggcgtgt tgctaaagca
    11881 atccgcacaa tcgtttacgg tgttcggatc aatgcaccga aaaaagtgcg tttgaatatc
    11941 atcgatgata aggaaaagta aataaacgtg ctgtactcga attttcgagt gcagcttttt
    12001 tgttttttta ttaattgaag aaatgattat ttggcaaaac aagcaagaaa gcgtatcctt
    12061 ataggaggag ggagtgaaca gccaatgaga aagaaaatgt tatttattcc atttgctcta
    12121 ttgttaagcg cttgtagttc ggttactcaa gaatcagaca atacacatct atcaagcagt
    12181 atatctgaaa ctgttaccag tccagaaaaa agtagtacca aaacggctac caccactgaa
    12241 caaacaatgg ctacctcaaa taatgagaaa aagactgctt tagaccagtt gaaagaacaa
    12301 cagccaaatg ttcccatgcc tctagatgta ccagtctctt caggctatct caatatcgca
    12361 gcgacacata caaagcaagg ttattctatt ttatattaca gaacagatcg tcccctcggg
    12421 ttgaatgctg acgaattgaa tcaagaaacg cctattgcaa cctatctata tcaatatggg
    12481 tttgcttcta gtcaagaaac aatccaagtt ttgcagccct ttgagatcga cacaaatggt
    12541 cagcaagtgg acttaggttc tcgaatcaca gggtatcagc aaggtgcagc aggttcaagt
    12601 tttcttgaat ggcaagaagg aaactggcgt attcgaatcc gtgggaataa tatagaaggc
    12661 caagatccct tactattagc taaagaaatc gtagcttatt tggaagaaaa cagtctacca
    12721 gcacctgaac aatttggtaa aattactgtt gatatgggtg atacgacgaa tcgagccgta
    12781 gaagtaagct ggcaagaacc gaaaaatgcc tatacgatca ctcatcaaga tcctatgtct
    12841 gcgttgaaga tggcagtttc tatgaagagg ttgtgaaatc agcgcgctga tctgagccca
    12901 tcatttaact ccaagtcaca ggaagtaatc aaaaatagca cgccgcattt ttgcataatt
    12961 ttggcttaat accttaaaag aaaactagaa ttgcctcttc acacatgcta aaataaaaca
    13021 gacaaaaaag aaatggggat ataagttgac tgaatacttg aatgtaggaa aaatcgtaaa
    13081 cacacaaggt atcaaaggcg aagtccgtgt tatctcaaca acggatttcc cagaagaacg
    13141 ttacaaaaaa ggaacggtgt taacactatt ccaagacgga aaagcacctg tggaattaac
    13201 agtcaaaagt catcgcaaac ataaaaattt tgacttattg agttttgaag gacatccttc
    13261 tatcaatgat gtggaaaaat atcgggatgg catattaaaa gtgtcaaaag acaactctgt
    13321 agatctagct gaaaatgaat tctattacca tgagatcatc ggtctgcatg tattagaaga
    13381 tggaactgag ttaggtaaag taaaagaaat cttgtcacca ggtgccaatg atgtttgggt
    13441 cgttcaacgt ccgaaaaaag ctgatatatt gcttccatat atcgcttctg tcgtcaaaga
    13501 agtcgattta gaagctggag tggtccgtgt agaaattcca gaaggactga ttgacgatga
    13561 agattgatgt attgacttta tttccaagaa tgtttgaagg tcctatgggg gaatccatca
    13621 tcggtaaggc tgtagataaa ggattattag atatcaatat ctctaacttc cgcgattatt
    13681 cagataacaa acatcagaca gtagatgatt atccatacgg cggtggagca ggtatgctgc
    13741 tcaaagtcca gcccatctat gataacatcc aagccattga aaaagctgca ccggatgtca
    13801 aaaaaagagt aattcttctt gacccagctg gtaaacgatt cgatcagaaa atggcagaag
    13861 aattttcaca agaagaacac ttagtattca tttgtggtca ttatgaggga tatgatgaac
    13921 gtatccgcac attagtgaca gatgaagttt ccttaggaga ttatgtcctt actgggggag
    13981 aattaggtgc gatggtcatg atcgatgcaa cagttcggct tttgccagat gtgttaggga
    14041 atcagacttc agctcaaacg gattcttatt caaccggttt attggaacat ccccaatata
    14101 ctcgcccggc agaatataaa gggatgaaag taccagaagt attgacaaat ggaaatcata
    14161 aattgattga agaatggcaa ttaaaagaat cactaagaag gacctatcaa cgtcggcctg
    14221 atcttttgga aacacttgaa tggacacctc aaatgacgaa atttttagaa gaaatcaaga
    14281 aagaagaaca agaaaaaaca gcagatgaag ccaagtgact ttttctaaaa tattctttac
    14341 agtaggttga taatatgtta gtattttatg gtgagtgcaa aagcacttaa ctattacgat
    14401 attccgctat gagaaaagct cataagaata tttggaataa ggagaagaaa accatgaatc
    14461 cattaatcga agaattaaca aaagaacaat tacgttctga catcccagcg ttccgccctg
    14521 gtgacactgt gcgtgttcac gcaaaagttg ttgaaggtac tcgtgaacgt atccagttat
    14581 ttgaaggtgt tgttatcaaa cgccgcggtg ctggaatcag cgaaacttat acagtacgta
    14641 aagtttctaa cggagtgggc gttgaacgta cattcccatt gcatacacca cgtgttgcaa
    14701 aaatcgaagt agttcgctac ggtaaagtac gtcgtgcaaa attgtactac ctacgtgcat
    14761 tacacggaaa agctgctcgt atcaaagaaa tccgtcgtta atcattgtta tcagactgag
    14821 aaagaaccct tggtattcaa gggttctttt ttcttagcat gtaatttgaa caccctgatc
    14881 cagttttttt ctttaattat tggtagggga gaataatgga ttagaataaa tctacctata
    14941 agtagtagta aaaaaaagag cactatgata atatctttct taagagtaac cgctttaata
    15001 aaaaatccag aatattctaa cataaatgga gtattcttag aatcaaaagg agaaagaaga
    15061 tggtttatac ttatacggag caagaaaaaa tgaaagtttg agacacattc atgaatgatg
    15121 gaaaagtaaa acaagtacca agttcaacaa aaaagaaaaa catcctattg aaagaagtct
    15181 tgaaacgctt tgaccatgga gtgacttata cagaaacaga ggttaattcg atactgctga
    15241 acgtattttc tagtggcgat tatgtcgaac aaagacgtta tctaatcaca tttggctttt
    15301 tcaaaagaag ttcagacggt cgggcatatc agatgatggg aattgaaaat tagaagtttt
    15361 aatttatact cattgaaaaa agttcaagta tgcgatattg gttctacacc gcatacttga
    15421 actttttttg tcatctgtat ctataataca aagccaagaa cgttattcaa aatatggata
    15481 gctatgataa aagctaatga gcgattcgca gttaaataag caatcgtcag taaaatcgtt
    15541 ccaggagcgt acatgaaaaa atcaattgga tgcgttacat gaataaagat aattctcttt
    15601 tatttgtacg aataaatacc aaaacgtacc tcctaaaatt agattcaaag aagatagcaa
    15661 agaggaaaaa gatagttctg cagcagctat agcggcatgt ggattgctag aactttccaa
    15721 acaactgcct gtttcagatc ctcgtcattt cgattacgaa gagatagcaa tcaaaatctt
    15781 ggaagaatta cagaaaaact atacaacgaa acgagttaac ggatcaaacg gtctgcttct
    15841 ccatgccgtt tatgataaaa acagcttaaa aggtgtagat gaatgtgtga tctggggcga
    15901 ttacttttat gtagaaggaa tcactcgtct agctaaaaca tggtactgtt actggtgatg
    15961 tataagagaa gaggaggatt catataatga agcgatggaa aaaaatgatg attggatcaa
    16021 tagttttagg aatgtcactc gtatttggtg cttgtggtaa tcaagaagac agtagtgaag
    16081 aatcagcaaa tgaaattacc gtttcgacat ggaattatga aacaacacct gaattcaaag
    16141 cgctctttga agcattcgaa aagaaaacgg gaatcaaagt gaaagcagta gatattgctt
    16201 cagatgatta tgatacgaaa ctcaccacga tgctttcttc tggagacacg acagatgtat
    16261 tgacgatgaa aaatcttttg tcgtattcaa attatgcttt acgagatcag ttagtcgatc
    16321 aaacagaacg aattagagaa ttagacactg atgctgctga agaaacatat gaaatgtatg
    16381 atatcgatgg aaaaacatat gcattgccat atcgaacgga cttttgggta ctatattaca
    16441 ataagaaaat gtttgatgat gcaggaatcg aatatccaaa aaatctaact tgggacgaat
    16501 atgaagattt agcaaaaaaa cttagtaaaa atgacggaca agtttacgga gcatatcaac
    16561 atatctggcg ttcgacaatc caagctattg cagctgctca aaacgaggcg aatcttgttg
    16621 aaccagatta tcaatatatg actgattatt atgatcgagc attgaggatg caaaaagaag
    16681 gcgcgcagat ggattttggc acggctaaat caacgaaagt aacctatcag tctcaatttg
    16741 aagaacaaaa agcagcaatg atgtacatgg gaacttggta tatggctggc atactcgcaa
    16801 acaaagaagc aagtaagaca gaagtagaat ggggaattac agctattcct cagaagaaaa
    16861 aaggagaatc ggtcacgacg tttggctcac caacagcatt tgctgtcaat aaaaactcaa
    16921 agaaacaaaa agcggcgcaa gaatttattg aatttgcagc cagtgaagaa ggagcaaaag
    16981 tattagcagg agtaggagta gttccttcat atcgtacgga tgagattgat cagctttatt
    17041 ttaatctcgc tggaatgcca acggatgaaa tttctaaaaa agctttttct cctgatgaaa
    17101 tcaaacttga atttccgatt gatacgtatg ggcctgcaat cgataagatt ttacaagaag
    17161 aatatgactt agttttggtt ggagatgaaa cgccggaaaa aggaacggta aatatggaga
    17221 aacgtgtagc tgcggaaaaa gaataatcat gaaaggagag aagccaagag ataaaacact
    17281 cttggtttcg tcatcgaaat ggagaatagt ataaataaga aagctacaaa tcatttgcgt
    17341 agaaaacgaa cgtggatcgc ttattcgttt atcgccccga atttcattgg ttttttcgtt
    17401 tttacattaa taccaatcct gttttcgctt gtactagcat ttatgaaatg ggattctttt
    17461 tcaacgccag aatttgctgg actagataac tttcgtaaaa tggccaacga tgagacattt
    17521 tggatttctt tgaaaaatac ttttttatat accgctgctg tagtgcctgg cacacttgtt
    17581 tgttcgttag ggctagcttt actactaaat cagaaaatta gagggatgaa gctttttcgt
    17641 acgatatttt tctttccgta tgttacttcc ttagtagcaa ttgctgttgt ttggaatatg
    17701 ttgtttcatc caacagtggg acctattaat atctttttaa gacgatttat tgaacatcct
    17761 ccaggttgga cttcaagttc aaattgggcg ctttgggcaa ttgtaattgt cagtatttgg
    17821 cgagggatgg gttattacat gattctatac cttgcaggac tacaaagtat ccaaaaagaa
    17881 ctatatgaag cgtcttcgct agatggagca aaccgttgga aacaattttt gaatgtgaca
    17941 ttaccttcgt tgagaccaac aaccttcttt gtaacgatca tgcttgtcat caattgtttc
    18001 aaggtgtttg accttgtgca agtgatgacg gatggcggcc ctggtcgagc gacaaatgtg
    18061 ctggtttacc aaatctacaa tgaagcattc aaaaaatttg attttggtta tgcttcagct
    18121 atttcacttg ttttgttcgt aattgttttg ggcgttacgg ttgttcaatt ttcttggaat
    18181 aagcgtcgta cttaggaggt gtgatatgga agagagtgtt cgaaatgaaa gaaaaaaagc
    18241 aaaaattcgt tcaaaaaaca tgccctttat tcaatggttg ggtatgattc tgctgattgt
    18301 attagcttta ttgacgatca ttcctttttt atggatggtt tctgcttcat tcaaagcaaa
    18361 taatgaagtg ttaacgattc cgattcaatg gatccctaaa acttggcatt tagagaatta
    18421 tctggttatt tggtctcgta ttcctttagt gacatttttt aagaatacag cttttttaag
    18481 cgttgtgatc acgttgattc agttgtttac ttctagtttt gctgtttacg ggttttctga
    18541 actaaatttt aaagggcgcg atattttatt tttagcatat atcggcacga ttgctgtccc
    18601 ttggcagtct tatatgatcc cgcagttcat tatgatgaga aaagtgggtt taacagatac
    18661 gttgtggtcg ttggtgctat tacaagcgtt caatgctttt ggtgtgttct tgttgaagca
    18721 atattacagt agtatcccag attctttatg tgagagtgcg cggatcgatg gcttatcaga
    18781 gtgggaaatc tatcgcaaga tcattttacc tcttacaaaa ccggcgattg ccagtttgac
    18841 gatcatcaca ttcgttaata catggaacga ttatatgggg ccattcattt atttgtcttc
    18901 tacggaaaat aaaacaatcc agttagggtt gaaaatgttt attgggctat ttgatgcaga
    18961 gtacgcatta atcatggctg cctcagttgt ttctgttctt cctgttgctt ttatattttt
    19021 agcgatgcaa aaatattttg tagaagggat cgcttcatca ggaatgaagg aataggaggt
    19081 gattatgaaa gagcagcgtt ttgacaaaaa tgtttatatg gaattctttt attggttgta
    19141 tcaatcattg gcaagtagcc tttgtttttg gtgtgtcaat atcttgtata tagcagctgc
    19201 gttccttttg tcgatagatg gaaggaatgt cttttccttt attttttctt tactttttat
    19261 tggtccaggt tgcatcgtga tgataggtat gacagaagaa ttagcaaaag aaaaaagttt
    19321 ttcaccagta cgtttgttca taaagacgtt tcgccaattt tggctaaagg gattttttta
    19381 ttggctattt gcttggatag ttagtgtgat aatgattttt gattgtttct tttttatacg
    19441 tttttcttat ggaaaatggt tgattccgtt gtttgtttta cttgcttgtt ttacttgctt
    19501 gtttatcagt ttcctttagc atcaactgct ggtattttca ggtgagaaat ccagcgtcga
    19561 aaccaaatca agttctacgg atagcgttct actatacgtt gaaaaaatgg tatgtgagtt
    19621 tactagattt tttactattg acaagtttgt ttctcttctt tttcgtaaag ccccaatggt
    19681 gtattctact aggtccttct atcgtatttg gtttgattta cttcaacaat cgtaaattga
    19741 tgagaactat ggatctgtag actttataaa ttgaaacttt caattaagaa tgacagtttt
    19801 ttatgtaaaa actgtcattc tttttcattt tttgaaacat atggctaaac aaatgaaata
    19861 aaacatgcta tacttttgaa tgaaacaggt ttagaaactg tatcacttat ttgtgattat
    19921 ttctaactaa taattaaaaa taaagcataa aaaggacgat gttcaatggc tgaaaaaatg
    19981 aaacaaggtt cgaaatctgt agtagaaagt ttgattaatc atcatgttga ttatgtattc
    20041 ggtataccag gtgcaaaaat cgatggtgta tttaatgaat tagaggatca aggtcctgaa
    20101 ttaatcgtga ctcgtcacga acaaaatgct gcatttatgg cacaagcaat cggacgtatt
    20161 acaggagagc caggagtagt tatcgcaaca agtggtcctg gtgctagtaa cttagctaca
    20221 ggtttggtaa cagcgacagc agaaggtgac ccagttttag caattgccgg acaagtaaaa
    20281 cgaagcgatc tattgaagtt gacccatcaa agcatggata atgcagcact gttccagcca
    20341 atcacaaaat acagtgcaga aatccaagat ccagaaacaa tttcagaagt gattgccaat
    20401 gcttatcgta tggctaagag ttcaaaaaaa ggcgcgagct ttatcagtat cccgcaagat
    20461 gtagtggatg cacctgtcca aggtaatgtc atcaagccat tgagcgatcc taaattaggt
    20521 tctgcttctg cagatgatat ccgttaccta gctgaacgca ttagagaagc aaaactaccg
    20581 gtactattag taggtatgcg aggatcaagt gaaaaagaaa cgttagccat caggcaatta
    20641 gttgaaaaga cagcattgcc tgttgtagag acattccaag cagctggagt gatctcacgt
    20701 gaattagaag cgcatttctt tggtcgtgtg ggtttattta gaaaccaacc aggagatatg
    20761 cttttgaaac gaagtgatct agttattgca attggctatg atccaatcga atatgaagca
    20821 agaaactgga atgcggaaaa agatgcgcgg atcattgtga tcgatgaagc gccagctgaa
    20881 atcgatcctt ttatgcagcc tgaaagagaa ttgatcggtg atatctcagc cactcttgat
    20941 ttattgacag gttcattaga gcctcaacaa gtgtcagaag atgcaaaaga atatttagca
    21001 tctcttcaag caaaattaac ggagcgggac attgttcaat caaaaggaga agcaggtatc
    21061 ttgcatcctt tagaagttat taacacgctt cagtcaaaag taacagatga tatgactgtt
    21121 accgtagatg taggtagtca ttatatttgg atggcacgcc actttagaag ttatgaacct
    21181 cgtcatttgt tattcagtaa tggtatgcaa acgcttggtg ttgcattgcc atgggcgatt
    21241 tctgctgcat tggtgcgtcc aaatacacag atcgtatcag tttccggcga tgggggattt
    21301 ttattctctg cccaggattt agaaacggcg gtacgtaaaa aattgaatat cgttcatttg
    21361 atttggaatg atggacacta caatatggta gagttccaag aaaaaatgaa atatcagcgg
    21421 gcgtcaggtg tagactttgg tccagttgat tttgtgaaat atgcagaagc ttttggcgct
    21481 aaaggaatcc gagctacaag tgtagaggaa ttagaaaaag ctttagaaga aggatttgcg
    21541 acagaaggac ccgtaattat tgatattccg attgattacc gtgataatga gaaattggga
    21601 gaaacaatct taccagatca attctattaa ggaagtgtcg acatgactga aaaaatctta
    21661 taccagcacg ggacattagg agctttgatg gccggcttga tggatggaac agaaacgatt
    21721 gctcatatat tagaaaaagg aactctggga ttgggaacat tgcatgggct tgatggtgaa
    21781 gtcatctttt tagatggcgt agcttatcaa ggaagatcag atggctcagt tgcccaatta
    21841 gatggaagtg aacttacccc ctatgcagca attacagatt ttacgccaga tactagtttt
    21901 tctgtctcaa aaactgcaga tggagaacaa ttgaaaaaag acatattagt aagagaagcc
    21961 ggggaaaacc tatttttggc agtgaagata actggattat tcaaaaatat gcacatccgt
    22021 atcatgccga agcaagaaaa accttacaga agactagcaa aaatttcaga gtctcaacca
    22081 gaattccaac aaagcaatgt ccaaggaaca ttagtcgggt tctttacgcc tgaattattc
    22141 caaggagttg ctgcagcggg atttcacctg cattttatcg atgatacaca tacatttggc
    22201 ggacatgtaa tggattttga agtggctgaa ggaaaagttg agattagccg catctcgtcc
    22261 cttgtacagc attttccagt tgatgatccg acttttgttc acgaaacgat tgattatgct
    22321 gatttagcag cagaaatcca acaagcagaa tagaaaaaga cagcgagatg cgttcaaggt
    22381 gattaacctg acgtatctcg tttttttgag gagaaaacct agcgtttaca aaaaaactga
    22441 gctataatct tctccataga tcaaaaagga gcggttttta tcatgtggac agaaaaacag
    22501 aaaaaagatg catacgaaat tttattatta cagcaacaag gattattaga agcagaagat
    22561 aactggttag caaatttagc aaattcatct gcattgttga atgagacttt gcctgagact
    22621 gtctttgcag gatattattt attcgatggt aatgaattga ttttaggacc gttccaaggg
    22681 cgagtttctt gcacacgtat caagatggga aaaggagttt gtggagaatc agctgaaaaa
    22741 caagaaacat tgatcgttga tgatgtcaaa acccatgcaa attacatttc ctgcgattca
    22801 gccgcacaat cagaaatcgt agtgccaatg gtaaaagaag gtaatttaat cggtgtttta
    22861 gatatcgatt gcggagtaac gaaagggtat gatgaaatcg atcaagcatt tctagaaaaa
    22921 tatgtagatc ttttattagc aaattttaat ataaatgctt tttcatttat taa
//
LOCUS       DAZUKC010000043        22780 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000043 DAZUKC010000000
VERSION     DAZUKC010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.044"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            69..635
                     /locus_tag="ACG80T_001597"
     CDS             69..635
                     /locus_tag="ACG80T_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4841239.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            780..867
                     /locus_tag="ACG80T_001598"
     tRNA            780..867
                     /locus_tag="ACG80T_001598"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:812..814,aa:Ser,seq:cga)
     gene            1203..2177
                     /gene="bsh"
                     /locus_tag="ACG80T_001599"
     CDS             1203..2177
                     /gene="bsh"
                     /locus_tag="ACG80T_001599"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFS4841240.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
     gene            2502..4397
                     /gene="asnB"
                     /locus_tag="ACG80T_001600"
     CDS             2502..4397
                     /gene="asnB"
                     /locus_tag="ACG80T_001600"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFS4841241.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(4592..5038)
                     /locus_tag="ACG80T_001601"
     CDS             complement(4592..5038)
                     /locus_tag="ACG80T_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFS4841242.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            5266..5415
                     /gene="rpmG"
                     /locus_tag="ACG80T_001602"
     CDS             5266..5415
                     /gene="rpmG"
                     /locus_tag="ACG80T_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS4841243.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            5503..6021
                     /locus_tag="ACG80T_001603"
     CDS             5503..6021
                     /locus_tag="ACG80T_001603"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFS4841244.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(6098..7054)
                     /locus_tag="ACG80T_001604"
     CDS             complement(6098..7054)
                     /locus_tag="ACG80T_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4841245.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            7220..8026
                     /locus_tag="ACG80T_001605"
     CDS             7220..8026
                     /locus_tag="ACG80T_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4841246.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8023..9012
                     /locus_tag="ACG80T_001606"
     CDS             8023..9012
                     /locus_tag="ACG80T_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFS4841247.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            9009..10013
                     /locus_tag="ACG80T_001607"
     CDS             9009..10013
                     /locus_tag="ACG80T_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFS4841248.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            10145..10687
                     /locus_tag="ACG80T_001608"
     CDS             10145..10687
                     /locus_tag="ACG80T_001608"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFS4841249.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            10707..11405
                     /locus_tag="ACG80T_001609"
     CDS             10707..11405
                     /locus_tag="ACG80T_001609"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFS4841250.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            11426..11638
                     /locus_tag="ACG80T_001610"
     CDS             11426..11638
                     /locus_tag="ACG80T_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFS4841251.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            11638..12600
                     /locus_tag="ACG80T_001611"
     CDS             11638..12600
                     /locus_tag="ACG80T_001611"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFS4841252.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            12618..13013
                     /locus_tag="ACG80T_001612"
     CDS             12618..13013
                     /locus_tag="ACG80T_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS4841253.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            13176..13541
                     /locus_tag="ACG80T_001613"
     CDS             13176..13541
                     /locus_tag="ACG80T_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFS4841254.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            13538..15349
                     /locus_tag="ACG80T_001614"
     CDS             13538..15349
                     /locus_tag="ACG80T_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFS4841255.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(15411..15578)
                     /locus_tag="ACG80T_001615"
     CDS             complement(15411..15578)
                     /locus_tag="ACG80T_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFS4841256.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            15827..16816
                     /gene="galE"
                     /locus_tag="ACG80T_001616"
     CDS             15827..16816
                     /gene="galE"
                     /locus_tag="ACG80T_001616"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFS4841257.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            16828..18312
                     /gene="galT"
                     /locus_tag="ACG80T_001617"
     CDS             16828..18312
                     /gene="galT"
                     /locus_tag="ACG80T_001617"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFS4841258.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            18336..19316
                     /locus_tag="ACG80T_001618"
     CDS             18336..19316
                     /locus_tag="ACG80T_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4841259.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            19507..20229
                     /gene="tsaB"
                     /locus_tag="ACG80T_001619"
     CDS             19507..20229
                     /gene="tsaB"
                     /locus_tag="ACG80T_001619"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFS4841260.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            20213..20755
                     /gene="rimI"
                     /locus_tag="ACG80T_001620"
     CDS             20213..20755
                     /gene="rimI"
                     /locus_tag="ACG80T_001620"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFS4841261.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            20762..21226
                     /gene="rimI"
                     /locus_tag="ACG80T_001621"
     CDS             20762..21226
                     /gene="rimI"
                     /locus_tag="ACG80T_001621"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFS4841262.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            21223..22239
                     /gene="tsaD"
                     /locus_tag="ACG80T_001622"
     CDS             21223..22239
                     /gene="tsaD"
                     /locus_tag="ACG80T_001622"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFS4841263.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
BASE COUNT         7239 a         3893 c         4830 g         6818 t
ORIGIN      
        1 gcaaaaaatg ctaaaaatag ggtacaataa aacaacatat actgcagtaa agaagtgggg
       61 gagttttaat gagaagaaaa gtctatacca aggatcaaat tctaaaagct gcttatgatg
      121 ttgttgcaaa ggaagggttt aaaggattta ctgcgagaaa tattgctaaa aagatgggga
      181 tttcgacaca gccgatttat ctggaattca aaaacatgga agatttaaaa aatacgttgc
      241 tggattctat ctttgaacaa ctaaaagaag atgttttccc tattgaacgt actggtgata
      301 aaatcattga tctcggtttg aactatatct actttgcaaa aaagcatcat agtctttaca
      361 tggcattgta cattgatgaa catggggacg gaaagaaaat gcatgatttt tcatacaatt
      421 atttccgtga aatagttaaa gaagatgaaa gatacaatca attatcggat gaatatgtcg
      481 atgctctgca tactggtaca tgggtagcag tgactggctt agcgtcattg atgtcatcaa
      541 acatcatgca tccaacagaa gaacaaatca ttgcactgat caaacggaca attgactcga
      601 ttttagcatt agataatcct gaaataatca gatagtgttc tatcagtatg aatttgatcg
      661 gtaagtccgt tggctgcaaa ctgtcaatgg acttttttgc taaaaaaagc gatttctgtt
      721 atttatctat tagaaatagg cttgacattt cgtgaaaaaa gtttaaaaat agtacttacg
      781 gaaggttggc agagtggtaa tgcaccggac tcgaaatccg gcgaaccggc taataccggc
      841 gcgcaggttc aaatcctgta ccttccttat atcaacgatt agaggttgtg aagaagctgt
      901 cctgcatcaa gtaataagaa cggccaaaca aaaatagctt ttcctatttt ttcgtttggt
      961 tgggcttatt accgcagatg caagcttttg aacaccgttt gttcggaata aggggctgtg
     1021 acaagacttt tgtcacagcc ccttattgtt gtacttttaa cctacaatat tttttgattc
     1081 cccctgagta ctatttttta taaatgctag taaatcaatc ttctttggcc ttttttttga
     1141 taaaaaaaaa tactagatgt aagataaaag tgtaaaaacg atttgtttta ggagggaaaa
     1201 cgatgtgtac cgcaatcact tatgcaacga aagaccatta ttttggaagg aattttgatt
     1261 atgagatgtc ttataatgaa gtagtcacta ttactccaag aaattatcgt ttcgatttta
     1321 gaaaggtaaa aaatttagat aaacattatg caatgatagg aatagcagct ggggtatcca
     1381 actatccact ctattatgag gctacaaatg aaaaagggtt gagtatggca ggactgaatt
     1441 ttcctggcaa tgctgattat aaagaattac aagaaggaaa agataacgtt gcaccatttg
     1501 agtttattcc atggatacta gggcaatgct caaccataga cgaagctaaa gaattattgg
     1561 ctactatcaa tctagtcaat atcgatttta gtgaaaaact acccttgtca cctttacact
     1621 ggttattagc tgataaagaa aaatcgatag taattgaaag tatgaaagat ggacttcatt
     1681 tatatgataa tcctgtcggt gtgttgacaa ataatccgcc atttgattat caattattta
     1741 atctgaacaa ttatcgctcg ctatcaaatg gaacaccaga aaatcatttt tcaaaccaga
     1801 tcagcttgaa tgtttatagt cgtgggatgg gaggattggg cttacctgga gatctctctt
     1861 ctgtttctcg atttgtaaaa gccacattta ctaaaatgaa tgctgcatca ggtgattcag
     1921 aatctgaaag tatcagtcaa ttttttcata tactaggctc agtcgagcaa caaaaaggag
     1981 tatgcgatac tggagaagga aaatacgaat atacgattta ttcttcttgc tgtaacgttg
     2041 ataaagggat ttattattat cgtacgtatg aagacagtca aattactgct gtaaatataa
     2101 ataaagaaga cttggatcgt catgaattgg tcagttatcc agtcataaaa gagcaacaga
     2161 taaactatgt caattaatta atgaatagat ccgtttggta tcatattttc tgatacagtc
     2221 ataattagac acgatcttta ggtcgtgtct ttttttgctg ttttttttgg aaatagcatc
     2281 cactaatatt tttttaacac gaagagaata aggtagcctc aaaaaagaaa acatggtaaa
     2341 ttagatattg ggactttatt ttctgtaaaa acagaaactt tcatcttttg ttttcacgtg
     2401 acgaaattgt aagagagaaa atattcgtcc gctatttatt ctatgataag atgcatatga
     2461 atgaaataaa cagtaatttt atattaggat gtgaaagtta aatgtgcggt attgtaggtt
     2521 ttgttaacga caaggacaac aaaaaaacaa ttattaatac aatgatggat cgaatcgttc
     2581 accgtggacc taatagttca ggagaatata tcgataaaca tgttgcttta ggattcagaa
     2641 gattaagtat tattgactta gagggcggta ctcagcctat ttacaatgaa gatcggacaa
     2701 agatcattat ttttaatggc gaaatctata attaccagcc tttgagagaa gaattgattg
     2761 cagctggcca cgtttttcaa acacatgcag atacagaagt ccttttgcat ggttacgaag
     2821 aatggggaac agaattattg caaaagatcc gcggaatgtt tgcttttgct atttgggata
     2881 atgaaaagaa tgagttattc ggtgcaagag accactttgg gatcaaacca tattattatg
     2941 cagaaatgaa cggaacattt atgtttggtt cagaaatcaa aagttttctg cctcatcctg
     3001 attttaacaa agaattgaac aaagaagcgt tgaaacctta tatgactttc caatattctc
     3061 cattaaacgg cgaaacattc ttcaaagatg tctatcgttt gcctgaaggc cattattata
     3121 cgtataaaga tggaaaacta gacattcagc aatattggga tgcagatttt gaaacaaaag
     3181 aaacgcattc ccgacaagaa tggatcgaaa aaatcgacga aaccgttcaa gcttctattg
     3241 aagcacatac ggtcagtgat gtagaagttg gttctttctt atccagcggg gttgattcca
     3301 gttatgtaac ctctgtattg aaaccagatc attctttctc aatcgggttt gatgataaaa
     3361 cctataatga agcgattgaa gccagaaaac taaccgaact attggactta gataatacag
     3421 cagcagtgat cgacggagac atgtcgttca aagcattccc tttgatccag tatcacttag
     3481 atgaacctga ttcaaatcct tcttgcgtac ctttgtactt tttagcaaat cttgcttcac
     3541 aaagtgtgcg ggtagtccaa tctggagaag gggcggatga actgtttgcg ggataccaaa
     3601 cgtatggatt ccatacgaat tcaaaattca tccgtgtgat tgctcaagga ttgaaaaaat
     3661 taccaaaagg aacacgctat aatttaggac gtaaaatcgg aaaaatgaaa aatttccatg
     3721 gacggatcca tttatatgaa tcacttgctc ctgcaaaaga atattttatc ggtcatgctc
     3781 gagtgttcga agaatcagaa gcatcagaag tgttaacacc aaaatatcaa acagcaccat
     3841 ctgtggatga aatcatgact gttcattacg agaaaacaga aggaatcaaa gatgaagtca
     3901 ataagatgca atatgtcgat cttcatcaat ggatgccaaa agatatcttg ttaaaggcag
     3961 ataaactttc tatggcaagt tctttggaag tccgagtacc tttattggat atcgaagtca
     4021 tgaaattggc tcaacaaata ccaagtaagt ttttattgaa tcaaaacaac acaaaagata
     4081 ttttcagaca agctgcgaac aaacatcttc cggaagaatg gtcgaatcgt gtgaaattag
     4141 gattccctgt gccaatcaaa gcttggttga aagaagaaca cggctacgaa caagtcaaag
     4201 cattattcga agcagatttt gcaaaagaat tttttgatca agaaaaaatc atgaagttgc
     4261 tagatgacca tcacgaagga cgtaaagaag agcaaagaaa aatctggaca atcttcagtt
     4321 tcttgacatg gtataaagtc tactttgtag atgaaacgat tccgcaagca gaagcaatcg
     4381 actatgtgac tgtttgatga aaatcaaata tcaataaaaa agaggttttc tcctaccgtt
     4441 taacactgag tgataagaaa caatttcaaa aaacagctct tcccgtttta tagatattat
     4501 gacttatggt tcggagttgg cagaggaaca ccattgtttc gaacataagg gggttgtgac
     4561 acgattttcg tcacaacccc tttacatatg ttcaataatc gatttctttt tcttttgtcc
     4621 catctttttt ataaatgaac aaagttgatt ctttgttttg tgctacttgc tttccttttt
     4681 cgattgcttc tttttttgta tcaaaatggt tgcttgcctt ttttgcccct ttagatttca
     4741 cgatccattg ttcttcctca tattctacga tcacatctga atcaagcagt tttccagcac
     4801 gaggattcgt atcgtgttca tccgtcttgc ttggattttt ttctttttcg aaagttcgtt
     4861 tttctgattc actagcatct tcataccatt tttttgcttg atcaatggca attgggatcg
     4921 cacggttatc agggtaccct tcatctaata atgcatttgc gatgtcgatt gctttttttc
     4981 ttgtcaattt atctaagttt ttcattgatg caggaaagtc cttcatgttc catggcatat
     5041 tagtatcccc ctttattaaa aaacataatc tatataaaca agtgtaaggg tatttataca
     5101 aaaagaaaaa gattttgttt gagaagtaaa accgttgtat ttatcgtcaa gtagttttac
     5161 ttaacaaaat cgtgaaaaac actagcaatc tagaaaaaat catggtaaga taatcaagtc
     5221 tgagagtcat cttagaggat tcttgtaaaa atgggaggga aaaacatgcg cgtaaacatc
     5281 actttagagt gtacttcttg taaagaacgt aactatctaa caagcaaaaa caaacgtaac
     5341 aatcctgatc gtttggaaaa acaaaaatat tgcccacgcg aacgcaaagt tactttgcac
     5401 cgtgaaacaa aataatgttt ttaaagttct caattccttg ctggtcaaag aattgagact
     5461 ttttttatct caaaacatct aaaatcataa ggagcggata atatgtttat ttccatgtgg
     5521 gcacaagata aaaatggtct gatcggcaaa aatggtttgt taccatggcg attgccaaat
     5581 gatatgcgtt tctttcgcga acatacaatg gatcgaattt tagtgatggg aagaaagacc
     5641 tatgaaggaa tgggggatct ttcacttcct tatcgccata tcatcgtgtt gacaacgcaa
     5701 ccaaattttc gcacgaaaga aaatgcggaa gtaatgcatt cgatcgatga acttttgtcg
     5761 tatagtcaaa cgatctcaga agatatctat gtttcgggtg gtagtcggat ttttcaagaa
     5821 cttcttcctt acactggtcg gatctggcga acattgatcg atagcacgtt tgaaggcgat
     5881 acgtatattg ggaatattga tttttcagac ttcgccctta tagaggagta tgaggggata
     5941 acggatcaag aaaatctata tgcccatcgt tttcaaaaat gggaaagagt aaaaaatagg
     6001 gttgcttcaa aagaagaata aaatctatgc gtctagtttg ttaataagaa aaaaagcatc
     6061 tcacgactaa tcgtgaagat tcttttttta gttaaaatca atttagtttt tctttgatat
     6121 cattgaccat ttcctgactg gcaataggtc cgttatatag ccaactagat tcaggtccga
     6181 attgcagtac atgattgtct gtcactgctt taagattctt ccagcttggt tcattgaaca
     6241 tggcagcatc tttgtcactg ttcacaagga tgatgtagtc agcatctaat tcagctagct
     6301 tttcagagga aacagcagac cagtcagaag tggctttttt ggagacctct tccactagat
     6361 caggaacacc aaattccaga tcgtcgtata cgattcttcc acttgaccga tttttgctga
     6421 ccataaaggc tgagttgttg gtcacccaca acactgcagc tgtcttgttc tcgattttat
     6481 ctgctagttc ttctcgagtt ttcttgatgg atttttgata atcggaaatg attttttctg
     6541 cctctttttc ttttgccaaa gctttgccga catcttttag ttgttcttcc catgtgacat
     6601 ctgttccgtt ttttacaaca taagttggcg cgattttgct atattgttcg tatttcccgc
     6661 cttcgacagt agcggaagaa gaaatcaaca gcaaatcagg ctcaaagctt aacactttct
     6721 cataaggaag gtcataagaa atcgttggga catcctttaa atcatcttgc agataatctt
     6781 gaatggaacc actgccaaca gtccactggg cgataggttt ttcccctagt gcaatcagat
     6841 agtcttctaa gtaactgccg atgatccgtt tgggcgcgtt tggaatctct actttatgtc
     6901 caagtgtatc tgtgactgta tgagtactat tggatgaagt cgtcgaattg gcagttgtag
     6961 ctgtttcttt atctgaagag ggggagcaag cgctcagtac tccagccagc agcaatatgc
     7021 tagataatgt gaatactttt gtttttaatt tcatttcttt cctcctaaca taattgagaa
     7081 tctttctcat tggttattat tcctgatatt tcgaacgctg tcaaccgctt catttggcta
     7141 tagatatgaa agtagcgctt gacgaaatgt tgaaacagcg tcataattga gaatgatttt
     7201 cagcgaaagg atgggtgaaa tgacgagatt aagagcagct gacattgcag ccggctacga
     7261 aaacaaaaaa atcattgaaa acatgtctgt tacgattcct gaagggaaaa tcactagttt
     7321 gattgggcca aatggaagtg gaaaatctac tttattgaaa agttttaccc gtatactacc
     7381 tgtgaaatca ggaaagatca ttgtcaataa ctctgatatc tctacataca gttcaaaaga
     7441 acttgcaaaa aaaattacat tattagcaca aactagtgaa caccctacag gtatgacagt
     7501 agaagaagta gtatcttacg gacgataccc ttatcaaaaa ctgttcagtg gcatggacac
     7561 gcaagatcat gaagcaatcg agtgggcgtt ggatgctaca ggtctagctg gatttcgtat
     7621 gcgggatatc gcaacgctgt ctggtggaca aacccagcgt gtctggatcg ctatggcgct
     7681 tgctcaggaa acagatatct tgatcttgga tgaaccgaca acatttttag atccggcaca
     7741 ccaattagag atcctacagt tattgactga aatcaatcaa atgaaacaaa caacgatctt
     7801 gatgtcgatt catgacatta atcatgcttc acgtttttca gattatttat tagcaatgaa
     7861 aaacggtaag ttgatcacag aaggtacacc tgaagaagtg atcacaaaag agtgtatgaa
     7921 aaaaatattt gagattgatg caacgattgt cacgcttcca agaacaaata aaccagtttt
     7981 tctaacctat gatttgttgc caaaagagca ggaggatcaa ttatgaggac tatgaagaaa
     8041 aatagactcc tgcttttggt ctgttttgtt cttttgatta ttttttttgt tgctctttca
     8101 aatggcgcag cgactgtggg aaaagcagat ttttggcagg cgttgtttga ttttgataga
     8161 tcaaaacaaa gtcaccaaat catacgaaat attcgtttgc ctcgtgtggc gggagcattt
     8221 cttgtaggga gttgttttgc attgagcggt gcattgatgc aaggagtgac aagaaatcct
     8281 ttagcagatt caggcttact aggaatcaac gcgggtgctt cgttggcaat ggcccttagt
     8341 tttgcttttt ggcctaaagc ttctgatttt tctgtgtttc taatgtcatt ggcaggttct
     8401 tttgctgtaa ctttgctcgt atttggtttt ttgcagtttt cgaaaatggg catgaatcct
     8461 ttacagctga ttttagctgg cgtagctatt agttcttttt ttactgctat cagccaagca
     8521 cttacccaat tctttaatct gcagcaggat cttgcttttt ggtttgtagg cggagctgcc
     8581 aatatcacat ggaaacaatt agcagtattg tttcctttat atatacttgc tgttttggtt
     8641 tctttttcat taggaaaagc aatatctttg attcatttga gtgaaacaca tgcattagct
     8701 cttggaagtc atcctaaacg aacgcggttg attgttttct gtctagttgc agtgttagca
     8761 gccattgccg tttcgttagt cggacctgtt tcatttattg ggctgttggt tccgccagtt
     8821 gttcgcggaa tggttggaac ggactatcgt tatgttctac ctgtctcttt tttagttggc
     8881 ggtggattgg ttatgttggc ggattttaca gcgaggatga tcaatccccc ttttgaaaca
     8941 ccttttggat tagtcattgc attgattggc gttccgtttt tactgataca agtaaggagg
     9001 gaacaacggt gaaaaaaata tattggacgt tctatttctt gctttttgta cttgtcatcc
     9061 tgtctatagg tagtttaatg attggtacgc cttttattta cctggatcag ctttttgcca
     9121 cgttcatagg gaaagaatca tcttctttgg cgctgattgt tagagaattt cgagttcctc
     9181 gattactgat cagtttatta gcaggagctt gtcttggcgt ttctggttat atcttgcaag
     9241 gagtaacaag aaatgaattg gcagattcta gtatattggg cattaatgcc ggagcaggat
     9301 ttttagtgat gctttattta ggtttctttt cgcactattc gctgccgttt ctattgctag
     9361 ttgtagcctt tctaggtggt gttttagctg cttgctgtgt ctatcttgcg gcctatgatc
     9421 gaaatggctt tttgggtatg aatcgaatct tattgtccgg tattgccgtt aatgccggcc
     9481 tttctgcatt aacattgcta tgcacgatcc aattatctaa ggaaaattat ggatttgtga
     9541 atgcatggtt agcgggatca atctggggtg cgagctggcc ttatgtttgg gcattgcttc
     9601 catgggcggt tatcttaatt ccgctaggag gactttatgc acagaggatt ggattgctat
     9661 catttggtca agaacgcgcg caaggattag gccttaacgt ggctaaaagt caaaaaatcc
     9721 ttttgttgtt agctgttgct ttggcaagcg gtagtgtagc ggtgacaggt agtttatctt
     9781 ttgtaggact actagctcct catgctgcga agctagtcgt acgtaaagaa aataactgga
     9841 cctttgttct gagcggtttg ttcggttcca tttttctgct tagtgcagat atggttgcta
     9901 gagtgattct accaagtggg gaagtaccta caggaatttt gattgccctt tttggtgcgc
     9961 catattttct gtatgcactt tttatcagac aaaaacacgc gctgaacagt tgaaatttat
    10021 taaaaaagga aggccaatca tttctaggtc ttctttttat tctatttatg catttatttg
    10081 aaagttcgat tgctattttt ctttttttcc tataccattt tatgagagaa ggaagggtga
    10141 gtttttggac caattgaaag taagattacg ccggaaagga atcgaaaagc tgaaaaatct
    10201 ggccaaaagt ggcaagaaaa aggaaaaaga acagcgaatc tataaacagc tattcgccag
    10261 cgagtactgg aagtcagcaa aagtgatagg gacgacttta tcgaatgaat ttgaactgga
    10321 tacacagccg gttatccaac gagcagaaaa agaacaaaag aaaatagttg tacctaaaac
    10381 tttgccgaaa cgtcagatgg cgttttatga aatggatgaa cagtccgttc ttgtacgttc
    10441 gagttttggt gttcttgagc cacaatcaga gaagctctat ttgccggaga tgatcgatct
    10501 cctcattgta ccaggtatta tctacaatag agccggttat cgaatcggtt ttggcggtgg
    10561 ttattacgac cggtatttaa ataattttca taaaaaaaca tgtagccttg tttttcgtga
    10621 acaattgaat gaacaatggc aaccggagag ctttgatcag aagatccaac aattgtttat
    10681 tgactaaaat gaaatgggag gttttcatga attatcaaca gcaaataaaa ttgcgtgcat
    10741 ggttaagacg accgttatgg acctatgctt ttttagggat acagacagcg gtcttcctca
    10801 tgatggaatt atttccgcga cttgagatcc cttattatgc cggaatgtat ggtccttatc
    10861 ttgttcactt caatgaatgg tggcgtttag tgacaccaat cttcatccat tttggactga
    10921 tgcatttcgt aatgaactca ctgatactgt attttatggg gcagcaaatc gaggcaatct
    10981 atggccattg gcgttttttc ttgatttata tgttcagcgg gataatggga aatacagcaa
    11041 gctttgcttt taacgaagcg aatgttctgt ctggtggtgc gagtacttcg atattcggtt
    11101 tgttcggtgc gttgtttatc ttagggtttc attttaaaca taacccagcg atccagcagc
    11161 tagtcagaca ttttctttta tttattgtgt tgacatttgt ttttggtctt ctggatacct
    11221 ctgtagatgt ttggggacat gtcggtgggc taattggcgg attagtgtta gggaatgttt
    11281 tgggtctacc gaaacaacag acaagctatt cgatacacca aaggattctt tctacactag
    11341 tttttgtatt tctttttgta atatgtattt tactgggttt aaaaaaatat ggtttacttg
    11401 tataatatga aggaagtgct taaagatgga gagcttatat gatgtacagc agctgttcaa
    11461 aaaatttggc atctttatct atgtgggtgc acgcatctat gacattgaat taatgactat
    11521 cgaattgcgt aatctgtatg atagccgttt gattgatcat gatacataca tgacagcgtg
    11581 gcgcatatta aaaagagagc accgaattga agaaagccga aagaaaggaa atttgtaatg
    11641 gacaagaaga ttattgggat tgatttaggt ggaacaacag caaaatttgc aattttaaca
    11701 ccagaaggag agattcaaca aaaatggagc atcgatacca atattttgga tgatggtaag
    11761 catatcgttc ctgaaatcat tgaatcaatc aaccatcgtt tgaatttata taatatgaaa
    11821 gccgaagact ttatcggaat cggcatgggt accccaggaa gcgtggacag cgaagctgga
    11881 acggttattg gtgcgtataa cttaaactgg acagaagttc aatttgtgaa aaaactgatc
    11941 gaggcaggaa caggaatcaa attcgctatc gataatgatg cgaatgttgc tgctttaggt
    12001 gaacgatgga aaggtgctgg agaaaacgat ccagacgttg tattcgttac tctaggaaca
    12061 ggtgttggcg gtggtatcgt tgctggcgga aatttgattc acggcgtgag cggagcaggc
    12121 ggagagatcg gccatatcac tgtggatcct gaagggttcg aatgtacttg cggtaaacgc
    12181 ggatgtttag aaactgtgtc tagcgctact ggtgtggtac gtctaggtcg ttacttggca
    12241 gaagaatacg caggagattc aaaactgaaa gcaatgttag acaacggtga agaagtaaca
    12301 agtaaagata tctttgaaat ggctcaagaa gacgatccgt ttgcattgat ggttgttgat
    12361 cgcgtatgct tctacttagg tttagcttgc gccaacttgg ggaatacatt gaatccgtca
    12421 agtattgtgt taggtggagg agtatcggca gcaggagaat ttttacgtag ccgtgtagag
    12481 aaatatttta aagaatttac tttcccgcaa gtaagagaat caacaaaaat caaattagca
    12541 gagcttggta atgaagctgg tgtcattggt gctgcctcat tagccttgaa atttgcttaa
    12601 aaaaacggag gcattacatg gttttatggg taattaatat cattttgcta ttgattgtgc
    12661 ttgcaatcat cttttgggaa gtctatctac gagtaatggc caagcgttca gcaacgactt
    12721 tgacagaaga agaatttcga gaaggaatga gaaaagcaca agtaatcgat gttcgcgaaa
    12781 aagatgtgtt tgatgctggg catattttag gtgcaagaaa catcccttac actgttttga
    12841 aagattcatt aggatctatt cgaaaagacc agcctgtcta tatttacgat caaaagaaaa
    12901 gcttgagcat ccgtacagca aataaattac gtaaagcagg ttatcaagag atttatttac
    12961 ttaagggtgg atatgacgga tggtctggaa aaatcaaaag taaaaaagta taaaacagat
    13021 gattgtatga cagctttaaa aaccgaatca atcattagat tttgaccaag atcacatgaa
    13081 cggcgcaatg gatagttgtg ccgttcattt tttttatttt gtgctattat ctacatatat
    13141 aagctaagcg aaaaggcaga aagaaggaaa caactatgat caaattaaaa cgtgtttacc
    13201 ttgatgctga aaaagaggat ggctatagaa tactcgtgga taggttctgg ccacgaggaa
    13261 tttcaaaaga aaaagcgcag ttagatctgt ggttgaaaga aattgcacct agtaatgaag
    13321 cacgcaaaac ttttgaccat gtaccagaac gatttgcttc atttgcaaag aaataccagc
    13381 aagaattgat tttcgaagaa gaaaagaagc aagctatttt taaactcagt caacttatga
    13441 aagaacatcc agtcattact tttgtttatg gtgcaaaaaa tgaacatcag aaccatgctg
    13501 ttttattgaa aaaatttatc gaaatggagg catgcaaatg aaaataggga tcattggtgc
    13561 cggacctaga ggattaagta tagcagaaag gattctccat aataatcagg acagacattc
    13621 tcttgaactt tttcttttcg atcccaatgg tcctggaggt cgagtttggc ggttgaatca
    13681 gccaccggag ttattaatga attctgtttc tcaacaagtg acgcttttta cagatgaaac
    13741 attgtccagt ggaggattgg taacacaggg acccaacttg tttcaatgga gtaaaggacc
    13801 tgctacagat tatatcgaac agacggtaac caaaaataag caactaatta tagaagaaat
    13861 caatcgtctg gaaaaagatc agcaatctac tcgatgttta tacggactct atcaacgctg
    13921 gtttttctca aagttgcaag aagaatttcc agcttctatt cagtttgttt attctttagt
    13981 aaaaaaagtc agcaaaagaa aagatggtta tcttttacag acagatcagc aaagctattc
    14041 tatcgatcag cttgttttag caagcggtca ctgggaaaat gatgagacag aagaagaaag
    14101 agtgtgggta gactacgcaa agaaaaatga tttactgtat cagcttcctt ctaatcctgc
    14161 agatgtcgat ctttcgaaga taccgcctaa agaaaatgtc tttttaagag gtctaggctt
    14221 aagttttttt gattatgtcg gattgtttac agtatcaaga gaagggcgct tcgagaacga
    14281 aaaaggaaaa cttgtctatc atccatcagg caaggaaccg attgtataca gtggatcaag
    14341 aagggggctc ccttattacc ctcgaggaag aaaccaaaag caaggcggat caatggcatg
    14401 gccacggtta ctaacaaaag aaaacctgga gcaatggcat cagtcgggat ttttgactgg
    14461 cgaaacattt tttgactatc tgaaaaaaga cgttgagttg ttttattaca agaaaatcat
    14521 cgaagaatat catttgccga ttccaaagaa aaattttgag catgattttc tctccctctc
    14581 ttcagaggag tgtctcggaa agcatccagc actaatccct tatagatggg attggtcgta
    14641 tttagaaatt cctcttacct atcaggatga gtcctttgca gaagctagcc gagcatttat
    14701 tgaagggcag ataaaagaag cggaaaaagg caattgcaca ggagcatttg cttcaacatt
    14761 tgatgcatta aaagactggc gtgatccaat ccggcaggct attgaatggc acgttttttc
    14821 tgcaaaggaa tacaaagaaa tactatgggg atggtttact catctaaata gctttttaac
    14881 gatcggtccg ccagtcattc ggacgaaaga attagcagca ctgatagatg cgggtatttt
    14941 tcatttgttc gaaccgccat ttgatattca gcaaaagcac aaccgctatg ttatttctct
    15001 acaagataaa gaaatcagca gtcgctattt gattgaagct cgattgccac aaacaagctt
    15061 ttctcgttca agtaatcctg tcataaaaaa tttgaaggaa aacaaacttg ttcgcccttt
    15121 ttcattttca gataatacag gaagttatca ttcagaagct atagatgttg agcgaggtac
    15181 gaatcgcgtg attgatgctt caggggaaat ccagtctgac ttttattgct atggaatacc
    15241 tgttgaaggt gtggattggc taactgcagc agtagctaga ccttatacag atccatggaa
    15301 tctgagacag gcagataaaa tagcacaact tatattgact gacaaataaa aatacgcatg
    15361 atgacttatc tgttctttca caagtcttca tgcgtgtttt taaataatta ttaacgagaa
    15421 acgcgtttgc gcattgcttt tttacggttt tcttcgatca ttgcttcttt ttcgtcaatt
    15481 ggatcgatca ctgttttttt cacagaagcg accaaaccag caacggcagc ggctgttgcc
    15541 agacttccta ctaatattcc agatacaaat tttttcattg aatccacctc atttattatt
    15601 tgttatatct attatggaac aaaagagaaa aaaaggctag ttttacgttt gtttgtaaac
    15661 ctttgcaaaa aatccttgaa aatgtttact aaatgtttta ttataaattt accatatgaa
    15721 tttacgaaat tttactttgt ttgttttcac cataaaagta aacttcgttt ttttaaatta
    15781 tatataatca acaaacacaa ttttactaat caaaggagtg ttttttatga caattttagt
    15841 actaggcgga gccggttata tcggttcgca cgctgtagac cagctagttc aaaaggggta
    15901 tcaagtagct gtggtagata atttgctcac aggtcacaaa caagcagtac atccagatgc
    15961 tcatttttat gaaggagaca tccgggataa agaatttcta cgttccgtat ttgaaaagga
    16021 accgattgaa ggagtaatcc attttgctgc aagctcttta gtaggagaat ctgtcgaaaa
    16081 accattgatg tatttcaaca acaacgtata cggtatgcaa atcttacttg aagtcatgca
    16141 cgagttcaat gtgaataaaa tcgtgttctc ttctactgca gcaacttacg gagaaccaaa
    16201 agagtctcca atcacagaag atacaccagc taatcctaaa aatccgtatg gtgaaagtaa
    16261 attgatgatg gaaaaaatga tgaagtggtg tgaccaagca tatggcatgc gctatgtcgc
    16321 tttgcgctac ttcaatgtag ccggagcaaa agcagatgca tctatcggag aagatcatac
    16381 accagaaacg catcttgtcc caatcatttt acaagtagct cttggtcaaa gaaaagcatt
    16441 ggctgtttat ggagacgatt atgatacacc agatggaaca tgtatcagag attatgttca
    16501 agtagaagat ttgattgctg cacatatttt agctttggaa tacttgaaag aaggaaatga
    16561 aagcaacttc ttcaatttag gcagcagcaa aggatactca gtcaaggaaa tgctagaagc
    16621 agctagagaa gtgacaggaa aagaaattcc agcagaaata gcaccaagaa gagctggaga
    16681 tcctagccga ttagtcgctt caagtgaaaa agcgagagag gttctgggtt ggaaacctga
    16741 atacacagat atcaaagcaa ttatcaaaac agcatgggat tggcatgtca gccatccaaa
    16801 tggttatgaa gaatagggga atgagatatg tcaatcagtc aaacgatttg tgattttgcc
    16861 acacttgcga tcgaagctgg cggatggatg gaaatggacc gcttatattt acagaatcgt
    16921 cttttaggga tggtaggaga agatgctcta gaaagcattg aagtaagacc agttaaggaa
    16981 gaatctgtag acttattgga tcagcttgtc gctaaggcaa gagaaaatgg tgttctttca
    17041 gaattgccag cagaacaaga gatttttgaa gcgcaattaa tggattttct cacaccgcca
    17101 ccttcagtcg tcaatgcatt ctttgcacag cactattcga aaagcccaga ggaagcaact
    17161 gagtatttct ttaatttaag taagaaaaat gactacatca aaacaagagc catcgcaaaa
    17221 aacatcaaat ttccggcaga gtcggagtat ggtgaactgg aaatcacaat caatttatct
    17281 aagccagaaa aagatccaaa acaaatcgca gcagaacgcg aggcggtaaa agtagactat
    17341 cctacctgca tgctttgcat ggaaaacgaa ggatatagag gtcgcttgaa ctatccagcc
    17401 cgcaccaatc accggattat tcgaatgaat ctagacggtg aaagctgggg attccaatat
    17461 tctccttatg cttattacaa tgaacattcg atcattttat cagaacaaca ccgcccgatg
    17521 aaaatcacaa aggaaacatt tgctagactg cttcggatta ctgaagtctt gcctcattac
    17581 tttgttggtt cgaatgctga cttgccgatt gttggaggat cgattctttc tcatgatcat
    17641 tatcaggcag gacgtcatga atttcctatg gcaaaagcac cgatagaaaa acttatcaaa
    17701 ctagcagatt atccgcaagt catcgcagga gtcgtgaaat ggccgatgtc tgtgatccgt
    17761 ttgcaatcaa cagagaaaaa attgttgatt gaagcagcta actcagtatt ggaaaagtgg
    17821 aaagcatatt cggatgaaac agtatctatc agagcttttt cagcagatgg tacaccacac
    17881 catacgatta cgccaattgc acgaagaagg gacaattact ttgagttgga ccttgtcctt
    17941 cgagacaaca atgtttcaga agaacatcct gatgggattt ttcatccaca taaagatgtc
    18001 caacatatca aaaaggagaa cattgggttg atcgaagtaa tggggcttgc tgttttacca
    18061 cctcgtttga aaccagaact agtagaagta gagcatttct tattaggaga agaaaaccag
    18121 atggctgaat atcatcgtcc gtgggcagaa caattaaaaa aagaacatcc tgatttgacg
    18181 aaagatacgg tcaccgatgt cgtacaaaaa tctgtaggtc aggtttttgc acgtgtactg
    18241 gaagacgctg gagtattcaa gcgagatgaa aaaggacagg cagcgtttga acgatttact
    18301 gcaattttat aaacgtaaaa aaggaggaga gacaaatggc aacaattaaa gacattgcgc
    18361 agttggcagg agtttctcct gctactgttt caagagttct caactatgat cctgatcttt
    18421 ccgttggctt agccacaaag caaaaaattt ttgaagcagc agaacgctta aattatacga
    18481 aacatcgtaa aaatgcagca aatactaagc aaacgcttcg cctgatccag tggtacgacg
    18541 atacggaaga gcttgaagat ctttattatt tatctattcg tctaggaatc gaaaaaaagg
    18601 cagaagaagc aaacgttact ttattgaaag aaacgtggga ttccattagt gatcttccga
    18661 cagatggaac gatcgcacta ggaaaattcg acgaagctca gttattagaa atgaaaggat
    18721 cccaaaatgc cttgcttttg gtagattctg acggcacttc tcaaggaatc gactctctag
    18781 ttgtcgactt taagtcaagt gttcaaaaag tcattgaaca ttttgtctcc cataaagcgc
    18841 aacatatcgc tatgcttgct ggaacagaat atacgaagaa aaaccatcag cggctaaaag
    18901 atcccagaat tatggcattt caacaagttt tggcagaaaa aggctacgac gaaggtccaa
    18961 ttatcgaagc agatttttct gtcgagtcag gatatcaagc agtgaagcag tatttaaaaa
    19021 cgaatccgca tgttcctgat gcgctttttg cagctaatga tgcattagcg attggcgcac
    19081 tgaaagcgat tcaagaaaaa ggtttagtgg taccagaaga tatttctgtg atgggtttca
    19141 atgatgtcag tgtagcgaaa tatgtaacgc cctctctttc tactgtaaaa gtttataccg
    19201 aatggatggg agagctagca gtggagacga ttcttcaatt aagtaattcc aaagcaccgg
    19261 tttcaagaaa gattattgtg gaaacagagt tgattattcg agaatcaacg aaataaaaga
    19321 tggaaaagct gtggggaaat gaagtgatac cgcagctttt ttgtgtttca ggaaggggca
    19381 aaatttgccc gccagcagat aatcatgtat cattgtacta gttttgcctt tggtattttc
    19441 atcctcagaa taaaaaagta tgctacaatg gaaggcgaga atgtcatagc agaaaggatc
    19501 aaatatatga ttacattagg aatagatact gcaaaccaaa ctttggcgat cggtgtggta
    19561 gaagacgaac aaattcttgg acaaatacaa acaaatatca aacgcaacca cagtgtaaca
    19621 ctgatgcctg caatcgacca attgtttgca gatcttcaaa taagtccaaa agacattgac
    19681 cgaatcgcgg tttctgatgg tcccggttct tatacagggc ttcgtatagg tgtaacgaca
    19741 gcaaaaacga ttgcttatac attagataaa gagctagtag gagtatctag tctgaaaacc
    19801 atcgcggcta attgcatcgg tgtggataaa attattgtgc ccatgtttga tgctcgaaga
    19861 aaaaatgtat acgctggggc ataccgctgg aaagaaaata gattagaaat tgtacttgaa
    19921 gatgtacata tttctgcttc tgatttgttc acacaactac aaaagctgaa tgaagctatt
    19981 tatttcgtcg ggagtgattg tcacaaattc ttctatgaaa taaaggaaat attgcctagt
    20041 gcgatgatta atacagttcc agcatgggat attccaagtg gtgtaactgt ggctcaatta
    20101 ggtgctttag ctgaaccagt tgcagagaaa caagcttttc ttcctcgcta tttaaaacgg
    20161 gtagaagcag aagaaaaatg gttggagacg cacacacctg gagacgaaag ttatgttgaa
    20221 aaaatttaga gcgtatgcca gaagcttttt tcaagataaa atgatttata cagaaaaaac
    20281 aatcgaacaa atcgatcgtc gttacgtact aagagaattg actacagaag atatcaaaga
    20341 gctgctggat gtcgaacgag aagtctataa tggcgaattg ccatggacaa aaagtgcctt
    20401 tatatcagaa ttgaagtcac cgcttcctca tctatatcta ggtatctttg ataatggaag
    20461 aatgattggt tttatcggtt caagagcgtt cggtttagat tgccatatca cgaatattgc
    20521 cgttcgaccg gcatatcagc gtaaacgaat cggttcgctg cttattgacg aaatcgagaa
    20581 ctttgctata atgaatcgct gtgagacgat gtcattagaa gtgcgtatga gtaatcaaga
    20641 tgcacaaaga ctttatcgga agttaggttt tcaagcaaca acgatcaaaa aaagatatta
    20701 cactgaaaca aaagaagatg cgttggatat gataaaactt ttagaaaatg agtgagatta
    20761 gttgatttgc agaaaaaaag attttgatga agcgtacttg gcagaatgcg tatggatgat
    20821 tagcgaaaat gcttatccac atggttcacc ttggacgaaa atgcagtttt tggcagacat
    20881 ccaacagcct cactctgatt atctgctttt agttgaagat aaccagatct gtggctttat
    20941 tggatactcg aaagtaatag atgaagtcga gatcacaaat attgctgttg cagtttcaga
    21001 acaaggaaaa gggcatgcac ggcatttgtt acaattactg attacggaac aaataaaaga
    21061 ttcgctgcat gtttttttag aagtacgtct atctaatgaa gcagcaagaa aactttatga
    21121 atcagaaaag tttcgtatat taggtaaacg gaaatcatat tatcgcaatc cgacggaaga
    21181 tgcgatcatt atgagcagaa agttaaaaac ggagacgatg aaatgaatga agaattgatt
    21241 ctggcgattg aatcaagttg tgatgaaacg agtgtagcgg tcgttagaaa tggaacagag
    21301 atcttatcta acatcgttgc ttctcaaata aacagtcata aacgttttgg tggcgtagtt
    21361 cctgaagtag ctagccgcca tcacgtagaa caaatcacct tatgcttgga agatgcactg
    21421 gtagaagctg gtgtgtcggc agaagattta tcggcagtcg ctgtcactta tggaccagga
    21481 cttgtcggct cgttgctgat tggtatttct gcagctaagg cttttgcttg ggcacaccaa
    21541 ttgccgctta ttcctgtaaa ccatatggca gggcatatct atgcagcccg tctggttaaa
    21601 ccattccagt ttccattgat ggcacttctt gttagcggag gacatacaga attagtctat
    21661 atgcaagaag atggatcata tgaaatcatt ggggagacaa gagatgacgc agcaggagaa
    21721 gcttatgaca aagttggaag agtattaggt ctttcttacc caagcggaaa agaaatcgat
    21781 cagctagctc atcagggaaa agataattac cattttccac gagcgatgat ccatgaagat
    21841 aattatgatt tcagttttag cggattaaaa agtgcattta tcaatttggt gcataatgca
    21901 caacagcgtg gagaagatct agacaaaaat gatttagcag ctagctttca agcaagtgtg
    21961 atcgatgtat tgattactaa aacacttcga gcttgtcaaa attatccagt caagcaactg
    22021 attgtagctg gaggtgtagc ggccaatcaa gggttgcgtg aaggattgca ggatgctttg
    22081 gcaactaaac tccctgaggt agaactagtg attccccctc ttcgactttg tggagataat
    22141 gctgcaatga ttggagcagc agcacatgtg gaaatgcaaa aaagaaattt tgctagctat
    22201 cagttgaatg ctgatccaag cttggtttta tctgaataaa gaggttgtga aaaatctgcc
    22261 ctgcatcaag taataagaac agcccaggaa aaacggcttc tcatgttttt gacctggctg
    22321 ttcttattac cgcgtatgca agcttttgaa cgccgtttaa tcaagagttc gtaagcctgt
    22381 cgtttaactc tgattcacaa ggaacaattt caaaaaacag ctcctcatgt ttatgaacat
    22441 tatgacttgt gaccgaagag ttgacaggta cacaccgttt aatcgaaata agggctgtga
    22501 cacgcttttg tcaaagccct attttttaca gcttattcat cattatccgt tcctctgatt
    22561 gccctcactg attaatcgtt tattgtcgag gaatatcgaa taatctaggt cggatgagga
    22621 caagacgctt cctctagtta atctatcagt attttttgca aacagtgaat attacataac
    22681 tgaaacacac atagaaaatc tttgagtctt gcccagtgat ctaaaactgc cggatctttc
    22741 agaagtttta gggaggaaaa taagcagtga ccctgataaa
//
LOCUS       DAZUKC010000044        22735 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000044 DAZUKC010000000
VERSION     DAZUKC010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.045"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..3660
                     /locus_tag="ACG80T_001623"
     CDS             133..3660
                     /locus_tag="ACG80T_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFS4841264.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="ACG80T_001624"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="ACG80T_001624"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFS4841265.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="ACG80T_001625"
     CDS             7448..7789
                     /locus_tag="ACG80T_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFS4841266.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="ACG80T_001626"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="ACG80T_001626"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFS4841267.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIVRKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /gene="pheT"
                     /locus_tag="ACG80T_001627"
     CDS             9146..11566
                     /gene="pheT"
                     /locus_tag="ACG80T_001627"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFS4841268.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACG80T_001628"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACG80T_001628"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFS4841269.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="ACG80T_001629"
     CDS             13000..13737
                     /locus_tag="ACG80T_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS4841270.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="ACG80T_001630"
     CDS             13751..14566
                     /locus_tag="ACG80T_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFS4841271.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="ACG80T_001631"
     CDS             14579..15226
                     /locus_tag="ACG80T_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS4841272.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="ACG80T_001632"
     CDS             15242..15898
                     /locus_tag="ACG80T_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS4841273.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="ACG80T_001633"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="ACG80T_001633"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFS4841274.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="ACG80T_001634"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="ACG80T_001634"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFS4841275.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="ACG80T_001635"
     CDS             18240..18758
                     /locus_tag="ACG80T_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS4841276.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /gene="cbpB"
                     /locus_tag="ACG80T_001636"
     CDS             18865..19365
                     /gene="cbpB"
                     /locus_tag="ACG80T_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFS4841277.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="ACG80T_001637"
     CDS             complement(19645..20565)
                     /locus_tag="ACG80T_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFS4841278.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20691..21743
                     /locus_tag="ACG80T_001638"
     CDS             20691..21743
                     /locus_tag="ACG80T_001638"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFS4841279.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="ACG80T_001639"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="ACG80T_001639"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFS4841280.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
BASE COUNT         7573 a         3958 c         4832 g         6372 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg tgcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaatttg atagtcgtaa aaaaatagag aataatagct aaaataactt
    22681 cttgatttct taattattag tcaaaatgtt ttgattgtca aactattttt tctta
//
LOCUS       DAZUKC010000045        22123 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000045 DAZUKC010000000
VERSION     DAZUKC010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.046"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..2743
                     /gene="clpB"
                     /locus_tag="ACG80T_001640"
     CDS             134..2743
                     /gene="clpB"
                     /locus_tag="ACG80T_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFS4841281.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2894..3580)
                     /locus_tag="ACG80T_001641"
     CDS             complement(2894..3580)
                     /locus_tag="ACG80T_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841282.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3558..4052)
                     /locus_tag="ACG80T_001642"
     CDS             complement(3558..4052)
                     /locus_tag="ACG80T_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFS4841283.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4072..5292)
                     /locus_tag="ACG80T_001643"
     CDS             complement(4072..5292)
                     /locus_tag="ACG80T_001643"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4841284.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5439..7274
                     /gene="lepA"
                     /locus_tag="ACG80T_001644"
     CDS             5439..7274
                     /gene="lepA"
                     /locus_tag="ACG80T_001644"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFS4841285.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            7470..8381
                     /locus_tag="ACG80T_001645"
     CDS             7470..8381
                     /locus_tag="ACG80T_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4841286.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            9377..9628
                     /locus_tag="ACG80T_001646"
     CDS             9377..9628
                     /locus_tag="ACG80T_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841287.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            9741..9983
                     /locus_tag="ACG80T_001647"
                     /pseudo
     CDS             9741..9983
                     /locus_tag="ACG80T_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10073..10312
                     /locus_tag="ACG80T_001648"
     CDS             10073..10312
                     /locus_tag="ACG80T_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841288.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            10459..14742
                     /locus_tag="ACG80T_001649"
     CDS             10459..14742
                     /locus_tag="ACG80T_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFS4841289.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            14841..15206
                     /locus_tag="ACG80T_001650"
     CDS             14841..15206
                     /locus_tag="ACG80T_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841290.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            15208..15660
                     /locus_tag="ACG80T_001651"
     CDS             15208..15660
                     /locus_tag="ACG80T_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841291.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            16109..17584
                     /locus_tag="ACG80T_001652"
     CDS             16109..17584
                     /locus_tag="ACG80T_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFS4841292.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            17607..17918
                     /locus_tag="ACG80T_001653"
     CDS             17607..17918
                     /locus_tag="ACG80T_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841293.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            18279..19535
                     /locus_tag="ACG80T_001654"
     CDS             18279..19535
                     /locus_tag="ACG80T_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS4841294.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            19532..20077
                     /locus_tag="ACG80T_001655"
     CDS             19532..20077
                     /locus_tag="ACG80T_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841295.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            20078..20578
                     /locus_tag="ACG80T_001656"
     CDS             20078..20578
                     /locus_tag="ACG80T_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841296.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            20937..21215
                     /locus_tag="ACG80T_001657"
     CDS             20937..21215
                     /locus_tag="ACG80T_001657"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841297.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVMQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            21235..21579
                     /locus_tag="ACG80T_001658"
     CDS             21235..21579
                     /locus_tag="ACG80T_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841298.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            21598..>22123
                     /locus_tag="ACG80T_001659"
     CDS             21598..>22123
                     /locus_tag="ACG80T_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFS4841299.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSV"
BASE COUNT         7599 a         3540 c         4670 g         6314 t
ORIGIN      
        1 tttatataag tcgagaaaaa aagtcaaaaa actatgtgta tattttgacc aaaattgacc
       61 aaagcgatat aatagttttt gtaaagatca aagaaataag tgatcttacc tttaattagg
      121 agggtatacc tgtatgaata ttgagaaaat gactacgacg cttcaagaag cgattgctga
      181 agctcaacag attgcggtca ctcgaaaaca tcaagatatc gacattgccc atgtatggaa
      241 aatcttttta caatctaatc attttggtcg aaatttctat acggatgccg gattggatgt
      301 cgaatctttt gaacatgaaa ttgatcgtct actggatgag tatccagttg tttctggtgg
      361 aaacgtccaa tatggacaaa acctcagcca gaatttcttt cgattattga atgaagcaga
      421 ccaaatacgt gaaagcttcg gcgacgagtt tctttcaacg gaagtcgtta ttttagcgtt
      481 gatgaaatta aaaaattatc cgttaactgt ttatttgaat aaaaacggat tatctgaaaa
      541 agaattgcgt aaaaacatcg aagagatgag aggaggagaa agagtgacct cacaaaacca
      601 agaagaacaa tacaaagcat tagaaaaata cggcgttgat ttagtacagc aagtcaaaag
      661 cggaaaaatg gatccaatca tcggtcgtga tgaagaaatc cgtgatgtca tccgtattct
      721 ttcacgaaaa acaaaaaata atcctgttct gataggagaa cccggtgtag ggaaaacggc
      781 aatcgttgaa gggcttgctc aacgtatcgt acgcaaagat gtgcctgaaa atttaaaaga
      841 taaaacgatt ttttcattag atatgggtgc attgatcgct ggagcaaaat tccgtggtga
      901 atttgaagaa agactaaaag cagtcttgaa agaagtcaaa aaaagtgatg gacgtatcct
      961 cttgttcatc gatgaaatcc acaatatcgt aggggcagga aaaacagaag gaagtatgga
     1021 tgccggaaat ctgttgaaac caatgcttgc tcgcggtgaa ttacatttga tcggtgccac
     1081 aactttagat gagtaccgtc aatatatgga aaaagacaaa gcgttggaac gtcgctttca
     1141 aaaggtatta gtcaaagaac caacagtgga agatacgatc agtattctgc gtggattaaa
     1201 agaacgtttt gagatccacc atggtgttaa catccatgac aacgcattag tagcagcagc
     1261 tactttatct gaccgttata ttactgatcg gttcttaccg gacaaagcaa tcgatttgat
     1321 cgatgaagcc agtgcaacga ttcgggtaga aatgaactct atgccaacgg aattagatca
     1381 agttactaga cgtttgatgc agttggaaat cgaagaagct gcactgaaaa aagaatcaga
     1441 tgatgcaagt aaaaaacgct tgaaaaattt gcaagaagaa ttagctgaac ttcgtgaaga
     1501 agcaaatgcg atgaagatgc agtgggaaac agaaaaagaa gaagtcaatt ctgtttcagc
     1561 aaaacgtgct gaaatcgata aagcaaaaca cgaattagaa gatgcagaaa ataactatga
     1621 tttagaacgc gctgctgtat tgcgtcatgg tacgatacct caattagaaa aagaattgaa
     1681 ggaattagaa gctaaagcaa aagacagcga gattaagatg gttcaagagt ccgttaccga
     1741 aaatgagatt gctcaagtcg ttggcagatt aacgggtatc cccgtaacga aacttgtaga
     1801 aggtgaacga gaaaaactaa ttaaattgaa tgaaacactt cataaacgag tgattggtca
     1861 agatgaagca gttgatgcgg tcagtgacgc ggtaattcgt tcacgggctg gattgcaaga
     1921 cccaaatcgt cctctaggtt ctttcctttt cctaggacca actggtgtcg gaaaaaccga
     1981 attagcgaaa gcgttagctg agaacttgtt tgactcagaa gatcatatgg tacggatcga
     2041 tatgagtgaa tatatggaaa aacatgctgt gtctcgtcta gtcggagctc ctccaggcta
     2101 tgtaggttac gaagaaggtg gtcaattaac ggaagctgtc agacgaaatc cttatacaat
     2161 cgtcttgttg gatgaaatcg aaaaagcgca tcccgatgtc ttcaatatct tgttacaagt
     2221 cctagatgac ggacgcttga ctgattcaaa agggcgagta gtagatttca aaaatacggt
     2281 tctgatcatg accagcaata tcggttcaca gttactgctg gaaggcgtga ctgctgatgg
     2341 aacgattcca gaagcagtgg ctgaacaagt caacacgcta ttacgtggaa acttcaaacc
     2401 agaattcttg aaccgaatcg acgacacgat tttatttaca ccattgagcc tagacaacgt
     2461 gaaaggaatc gttgacaaaa tggtggcgca attggcacaa cgtctggaac atcaagaaat
     2521 cctgctgaca atcagtgatg aagccaaaac gtggattgct gaaaatgctt atgaaccagc
     2581 atatggtgca agaccattga aacgatttat cactaaggaa gtagaaacac cactagctaa
     2641 agaaatcgtt gcaggacatg ttatgcctaa atcaaaagta acgattactt tacttgatgg
     2701 acaacttcat ttcaaaacag aagagctaga agaaatcgtc taatcttcca ttgagactat
     2761 aaaagaaaaa aggggtctga cacaactagt gtcagacctc cttttttcaa ataaacagtg
     2821 ttccagaagt agtttcttcg aaaaataagc cgaaatttct tgaagctgcc cgcttctgtc
     2881 acaacctctt ttattaggta tttttcactt tttctaattt ataactttca aagtaacgct
     2941 catctttttt actttgagac catgcaaaca atcccattaa ggcagcacct atcgtatttg
     3001 tcagcaagtc gcccatcgta tccatcaatg ctgctcgtcc gacaaacagt gtgccatcag
     3061 aagcagcaaa acgttgaaga ttcatgccta agaactggtc gcataagaat tcccagaact
     3121 cccaaaacac gccgcaaagt ccagcaaacg caaagccgaa taagaggaac aaccaaggac
     3181 tggtttttga tatctcagca tctttcatca agtaaccaat caatccatag ccaagagctg
     3241 tcaggaccat tggactcacc gcatgtaaga ttttgtccca aaaagagatg atactgatca
     3301 aatgcatccc tgtgcctaaa aagacagaga tgaacaagaa aaaccagtaa aacaaaacga
     3361 ttgctgctgg tattttaagt ttgaaaactt tcaaaatgac ttgtggcatg aaaatcaatg
     3421 caatccctaa taaagattcc acgataaatg tgattccttg agaagtcgaa tatttttgtg
     3481 tgataaattc ataaaaatta tagcagacaa cgaatacacc aaaagcaagt aaagctattt
     3541 gcaccttttt gatttcttta atcactgaaa gtccctccat ctaaaaatct ttttacttct
     3601 gaaagcaact caatttctga agaaaccact tttccgttca atttgatcaa acctattgta
     3661 tataagttca ggtagtgaaa ttgattttca gctgtttctt gtagtgcagc gatttttttc
     3721 gggtgggttc cgccttgctg tcgagtatca gtatataagc cgatcactgg gatgcctttc
     3781 gcataagcca caccgatttc cgatgctacc ccggcatcga tcgtcagccc atctagtaaa
     3841 gcaatcatta aatcactagc caacactttt tctgtgtcag caagagcaat cattcgactg
     3901 tctgcataag cagttttgtc attgattgct tcattttctt gaggaagata gatcgaaagc
     3961 tcagaagaga gctctctgat ttctttaact aactgttggt tatataacaa atcactttta
     4021 gcaaacattg gtgcggcaaa ataaattttc atctaaacat catccttttt atcaaaaata
     4081 atcgctaaag taagcttcca tgtttggaat gatacgctct agtgacttca gtgtttcttc
     4141 atctgtttct aaccgttcga ttcttgccaa ataagaagca cggcgataat tcatcatcaa
     4201 gcaagtcaat gtttggatat ccagtcgaac ggattttcct ataggttcat ttaagacacg
     4261 gacttgtccc tgttcatccc atgttaaagc gaagacacca ttattccaag gagcgacagg
     4321 atcttcaatg atgaaatgaa aagcatcagc tgttccaaca aacggaaacc gttgaaggaa
     4381 ttcttttaca tcaacgatcc gagccataaa atatggttcg atctgctctt tgatctgact
     4441 atcttcaatc aaaaatgcta aaggctcatt tttataaata tctccatgta cccagtagat
     4501 catggaaaaa tgtgcggaaa taaagttcca taagccattt cgagcttctt gatcaagata
     4561 gaacatttct tttacgtgaa atatttcttc agctacccag tagaacagaa cacctcttgg
     4621 ttcatggttg ctatcgtagt agacagcggc tgtgcgctca tcttcatttt cgaaacgcca
     4681 atactcttcc caatgaaaat cactgcggaa taaagcacca tggttttgtt gggcaaattt
     4741 gtcataaacg gaaaatacgt ctggatgatc aactggtaat cgctcaacca taccacttac
     4801 tgggatttgt ttaggtagct gtgtatctct gattttaaag gaaagtttat cagacatgat
     4861 ttcccatcct ttacgccgat agtaggggat actgtaagga tacaaataag agatccattg
     4921 cttattcttg cgcatattat ccaatgcaac gataattaga tcttgcatca atccatggtt
     4981 agcatattct ggataagttc caactccagt cacaccaccc attttataac gtgcaccgtg
     5041 aatattgact tcacaaggat agatagcgat ttgagagatc agtttatttt cgtggaacca
     5101 tccgaacact ttcgatactt ccaaaatcgg ttttttggaa cggataaaag ctcgtttatt
     5161 ctcaaacccg ctttcctcga tgtctgcttc agtcacttgg aacacatagg aaagcaattc
     5221 attgaattga tcgatatgtt cttgagtgac cggttttaat tcaagatttt ctctaaaatc
     5281 tttttgatcc attttttcat ctccatttat tagcttgata acagtatagc aaaattctac
     5341 aaaaaaaact tctatagctg actaattaaa aactctttaa attactgctt ttcgttggta
     5401 taatggaaag tgctgattat atgaaaagag gagacgctat ggatataaag aaaatgaaag
     5461 aacgccaaga gcagattcgt aatttttcga tcatcgctca tatcgaccat gggaaatcta
     5521 cattggccga tcgtatttta gaaatgacgc acactgtgac gtctcgcgaa atgcaagatc
     5581 agctgttaga ctcgatggat ttggaacgtg aacgtggaat cacgattaaa ttaaatgctg
     5641 tagaactcaa ttacacagcg aaaaatggag aaacatatat ctttcacttg attgacaccc
     5701 cagggcacgt cgacttcacc tatgaagtct ctcgaagcct tgctgcctgt gagggtgccg
     5761 ttttagtggt cgatgcagca caaggaatcg aagcacaaac attggccaat gtttatttag
     5821 cgttagacaa tgatttggag atcctgccag tcatcaataa aatcgaccta cccgcagcag
     5881 atcccgaacg tgtccgtcaa gaaatcgaag acgtaatcgg aatcgatgct agtgaagctg
     5941 tattagccag tgccaaatca gggattggga ttgaagatat tctcgagcag atcgtggaat
     6001 atgttcctgc accaagtgga gacttagatg caccgctaaa agctttgatc tttgactcta
     6061 tttatgatag ttaccgcggt gttgttctaa atgtccggat cacggacggt atggtcaaac
     6121 ccggagacaa aatcaaactg atgagtaatg gcaaaacatt tgatgttaca gaggtcgggg
     6181 tcttctcgcc aaaagcagtt gctagagact ttttgatggt cggtgatgtc ggttatatca
     6241 cagcaagtat caaaactgtc caagatacac gagtagggga tacagtaacg ttagcagaca
     6301 atcctgccgc tgaagcttta ccaggttacc gaaaaatgaa tccgatggtt tattgtggtc
     6361 tttatccaat tgatacttct cgttacaacg atttgcggga agctttagaa aaactgcaat
     6421 tgaatgatgc tgctttacaa ttcgaaccag aaacctctca agcattaggt tttgggttcc
     6481 gctgtggatt cctcggactt cttcatatgg atgtcgtcca agaacgcttg gaacgggaat
     6541 tcaatctaga attgatcacg actgcaccat ccgttattta ccatgttaat aaaacggatg
     6601 gtacgactgc tacagtagat aacccggcag attttcctga acctgtaacg attcaggatg
     6661 tagaagaacc attcgtcaaa gcacagatca tggtcccaaa tgattttgtc ggtgcagtaa
     6721 tggaactttc tcaacgaaaa cggggagaat tcattacgat ggattattta gatgattacc
     6781 gagtaaatgt cgtctataat attccgcttt ctgaaattgt ctttgacttt ttcgacaaat
     6841 tgaaatcaag cacgaaaggc tatgcctcat tggattatga aatgtctggt tatcaaaaaa
     6901 gcaagttagt gaagatggac atcttgctga atggagaaaa agtcgatgca ttaagcttta
     6961 tcgttcaccg agactttgcc tatgaacgtg gaaaagcaat cgttgaaaaa ctgaaaaaac
     7021 taatcccacg ccaacaattt gaggtgccaa tccaagcagc tattggacaa aaaatcgttg
     7081 cccgttcaga catcaaagcc ttgcgtaaaa acgtcttggc taaatgttat ggtggagatg
     7141 tttctcgtaa acgtaaactc ttagagaaac aaaaagaagg gaagaagcgg atgaaacaaa
     7201 tcggctccgt cgaagttccg caagaagcct tcatggccgt tctgaaaatg gatgagcaag
     7261 atagtgtgaa ataacttata atcaatcgaa aaacctatta aataaacctt tttgagtaga
     7321 tgcaacactg aggattatct gtataaaaga ttttaaactg tcatttggtt atcacggcga
     7381 tactaaaatt aacaaaattg gaatattttt cttgaagatt tttagctgtc agtatatgat
     7441 gaaagcatct cataggaggt gcttttttca tggttgataa ttcaagtgga agggttctta
     7501 aatctctaag aaaagagaaa aaactatctc aaaaaaagtt agctgattta gcaggtattt
     7561 cacaaagtac tttagttaaa tatgaaaaag ggtcaagaaa aatccctaaa gacgtagata
     7621 atactctaag caagattctg aatatagaaa ctctaataaa agatgaggaa gataagattg
     7681 agattctcat cggtaaacta attgcataca gagatatgaa taagttactt aacaaagagt
     7741 tagctgataa tataggaatt agtgaagtat tactcagtta tgtgttaaat agaaagagaa
     7801 atccgtcaaa agaaatgcaa aaaaaaatcg atatttttct attaagtaat gaaaaagaaa
     7861 ttttgaagga gattaatcgt gatagtgaga tatttagttt atcaaaagac gataaaattg
     7921 ttatgggaaa aagaatacga gaagtccgaa agaaccggga agagacgctt gaaaaatttg
     7981 gtaaaaactt taccatctat acaggcaaaa atgttataag tagatgggaa aaaggtatta
     8041 atattccaga tattgaaaaa ctaatgaata ttgcctatct tggtaaggtc actgttcctt
     8101 accttatgta cggagaagat tataagaata tacttccaaa agatgaaaga gttagcgatt
     8161 ttaaaaagat aaattctttt agtatgggat taaggatgag gaaaattagg aaagattatt
     8221 atctagaaag ggaggagttt ggaaagcttt tttctccttc aatttcaaaa tggtccatag
     8281 ataggtatga gaatggtaga gatataccca atactaatcg aattatccaa tatgcttata
     8341 taggaaatct ttctttagaa tttttgattt acgggatata aaaatagttt acgtcgatac
     8401 ttattaagtt atcgacgttt tttattatag aaaaaattta taaaaggagg tttcttgata
     8461 tgataagaaa caaaaagggt ttgttgaaag ttgctttact ggcagctttt ttctaagtaa
     8521 ctttttgaca agctcaatgg caacacaagc ggaagaagta atacaagaac taactgttat
     8581 gaataacagc tagataaaat gggctatgta tgttatccaa caatttagta atcactagaa
     8641 gcctagcgaa agttcgcttg ataagttata gatgagtatt gatttgtagt gtttccaaat
     8701 caaaagggta cagagccaca tgaaatatgt tgtcctctgc agcgtcagga tgggaatcca
     8761 taccatggag attcggagat ctgtgaggga atacttacgt ggttaatatg tcagttgtgt
     8821 tggacgtagt aatggtcata gtgggtagat tgactgaatt cttatatgag aaaatgaaac
     8881 caacaagaag atagggttat ttatacagaa ttggggatct tttctggaaa agtaatcgga
     8941 taatgcgaga ttcgtacggt cttttttaaa tttctctaaa aaaaacggca tcgaatgggt
     9001 tgggtgattg ataagcaatg aatcatagac tgatatagaa taaaaaatga ttctatttat
     9061 ctttttacat ctatcgtttt ttcaagtagc cgcaaattat atcagcattc aacaattttt
     9121 gtatacaagt tctagtaaaa ggatctgaag aaaaagttgg ttgttgtgaa gataaaaaaa
     9181 gaatcaggtt ttactgagtt tttcttcaaa atttgtgtta ttaggttagg acaaagataa
     9241 gaagaagtat gaagatttaa aaaaggaatg aggaaaagat agtaatttcc gattattgga
     9301 gtttttttat ttataaagaa gaagcgaata agttgtgatt cctagatttc gcgcgtgatt
     9361 aattaatgag caaaaaatgc ggaaagtttt agtgtttaat acacgaggac aaagtttctt
     9421 aggtattgaa ggcacacgtg taacggttga tgggtggtgt gattgtttcg aggttggaaa
     9481 acatgttgaa cttatggcat ttactgagct aaaagataag aacaacgtgg aatttttcga
     9541 gagtgatatt ggttgggacg atcatgaaga agtttatgga aaatttattt tgcggaaggg
     9601 aaattcttat acgaatgggg aagcataagt agcgatttgt tagagatcat tgataggatt
     9661 gaaattgtcg ggaatattta tgagaatcta gagttatttg aggtgataga atgagtgatg
     9721 gttttgaaca agtatcaata attaaaaatc aagttcggga gattcttaga aaaaaaggct
     9781 atttggtaga tagttatttt gaaggagatt acgaaacatg ggtaggtgtc tatgctcgtc
     9841 cagagaataa acctacctat ttagatccta cgacgtcaga agatgcctat ttacaaaata
     9901 ggtatcgagt agatggtttt aagcaagact ttgcggagtg gtttgaatgg gaaattgaaa
     9961 acggagaagt aaaagaagaa taagagtact tgttgtatca aaataagcca gtagaacaac
    10021 caaaccagta gctagtaatg acaataatac tgaggaatcg gatcattatg gtatgattga
    10081 tatttctctt ccagaagatc ttaagccaga tcctgtaaca attggcaata gtgtcatgat
    10141 ttttgattca ttagatgaag ctatgtcata tggttggaaa gaaattaagg atgaaggtag
    10201 taattggtat ggcatgagat ttggtgatgg tgaagttgcc aaaaaagctg gtattccaat
    10261 tgggaaatgg acagttgatt tccacgaagc aggttctttt tttgttctgt aattatagtg
    10321 ggagggtaaa atcattgatg aaatgtgttc agataagagc gccagataaa aatagaagag
    10381 acagttcacg tcgttatcac ttttggtaac ggcgttttgt tttattatat aaaattctca
    10441 ggaaaaggag gaattcttgt gtatgcaaaa acgaaaatga attggttgaa agcactcgca
    10501 ttggtggctt tttctcaatt ctgtttcttg gtgggaactg ctcaagcaca tgcagaagaa
    10561 acgacggcta tacaacctac gatggaagcc attgcccaag tcgcttctga agtgacagaa
    10621 gtccaaccag cagaagccgg ggtcccagct ggtgaagcca ctgttgaaca ggcccctgtg
    10681 acagaagcgc caacggtcaa ttccaaaacg actctcgatc agacgattgg ggaaacacaa
    10741 cacccaggga atagtcctgg tacttcacaa ccagttgaag aaactgcctc aattacaacc
    10801 cctgcttcag aaacgtccac tctagaagga gaaaaagctg ccgaagcaac gccttctgtg
    10861 cagccagagt cttctgagaa acaagcggaa gaaaagaaac gaatagaaga aacagtggaa
    10921 caaccagcgg ctactgctga caaagaagga aaaaatgagg aaaacgttga aaaaacaatc
    10981 tcttcaaccg cagctagtca aaatcaacca acgaaaccaa acaaagaatc tacggctagt
    11041 attgaaaatc aaaagaaaaa agagcagaac attccaaaaa caggtacacc tacactgtta
    11101 gtaacagtac caaccaatcg ttcggttcgt gttcgccgtg caaccaatgc agctgtttta
    11161 ggtggtcgga aaattgattg gggcgaaaat attggtgtta cttctagtcc gaacaatcta
    11221 aacttcactt taaattatat ggttcgtttt gagattaatg gacaagtggc gttttgtatt
    11281 gagcctggtg tctcagccgc agaaggtcaa gtttatacag ctactgcttt gaaaaactat
    11341 ttgaaaaatt caaatgtacg gaaaaaaaca agcttgatct cttatttagg atatgtcaca
    11401 aataaagaca aatccgatga acaatacatt gcggcacaat ttatgatttg ggaagctttg
    11461 ggcacaaaaa tcccaatcaa aaatatccgt gttgattatg atcgtcgaaa agcagaaatt
    11521 caacgtttga ttaataactt tggtgaaaaa gcaactttta ataatcaaac acatacggta
    11581 aaaattggcg aaacgttgaa agtaaagaat aaaaatgatt ttttgaaata tgtgaaagac
    11641 attcaaaaac caaaaggtgt caatgtatct attcacggca atgaagtatg gattacagtc
    11701 acaaaagagg caccggataa agcaaaaatt caattgaata aaattaaagt tcaaggtgcg
    11761 ccattagcct atcgtcgtcc aggagctcaa acaattggtg tgttacatcc aacagaacct
    11821 aatgcgtctg ttattaattt acaaattcta aaacaaggaa acattcgtgt gtttaaagaa
    11881 gatgcggaaa ctggtgtgaa agcacaaggt gcagcttctt tagaaaatgc ggtctatggt
    11941 ttgtatcaag cagatgggaa aaagattaaa gaaattacct tgaaaaaaat taacggcaaa
    12001 gtacaggcag aaattaaaga tcttgatcct ggcaaatatg ttttaaaaga aatcaaagcg
    12061 ccaaaaggtt atgtgctttc tgatgaagca attgaaattg ttgttgaacc tggaaaaact
    12121 gtcaatgcga ccgcaaaaga tcaagtgatc aaaggaacca ttgatttagt gaaagttgct
    12181 aataaagatt tagtcgattc aacgaatcca gataacaaac caaaattagc tggcattgaa
    12241 atcactttga cgtcaaaaac aactggcaaa gtggtgaaaa ccgttgtgac agacaatgat
    12301 ggttatgcta cttttggtaa aaataccgtt gtctttgata cgtacattct ttctgaaacc
    12361 aaaggaaaag aagggtataa actatttgaa ccacttgaag tcacaatttc tgaaaatggg
    12421 caaactttcc attatgtttt agaagataaa gtaattgaac aacgtcttaa agtggtgaaa
    12481 gtggatcaag aaacaggaaa agtgattcca cttgctaata cagaattcaa gatttttgat
    12541 actttagcca atcgttatgt cacaatggag attccaaatg atacagaaac aacggatatc
    12601 tttaaaacca atgaaaaagg ctttttccta actaacggta cacttactta tggaaaagat
    12661 cgctatcgtt tggaagaaat caaagccccc gtaaattacg ttttgaatca aaacccatta
    12721 gtgttctcga ttaccaatga tacagaaccg atcaaagtga tccattttgc gaaccgtcaa
    12781 gcacggaaaa gtgtaaaatt attcaagtat gaagaatgga acaagaagaa aacgccgctc
    12841 gcaggtgtga actttacctt gtataacggt aaaaatcaag caattggtga atataccact
    12901 gatcaaaacg gggaaatttt agtgaaaggt ctagttgccg atgactatta cttcttggaa
    12961 aatgccccat tagaaagttt ccaaccaaat acagataaac aagcattcac ggttgcattc
    13021 aaacaaaatg cgccagatga tggggaaacg ttagtggttt ctgtacgtaa ttacttgatt
    13081 ccaccaaaac tagaaacaca agcgtcaaat aaagccgatg gtgaaaaagt ggttgatcca
    13141 ttagaaacga tcgtcattaa agacagtgtt tcttatacca acttgtttgt cggaaaagaa
    13201 tacaccattt caggagtatt gatgaacaag gcaacgaatg cgccaatttt acaagatggc
    13261 aaagaaatca caagtacctt ggcctttatt gcagacaaag tcaatggcac aaaagacctt
    13321 gaatttgtgg ttaatgctag tgttctgaaa ggtcaaacga tcgttgtttt tgaaaaactt
    13381 ttccgtgatg gcattgaagt tgcagcacat gcggatatca acgatgaagc ccaaagtgtg
    13441 aaagtgaata atccaaccgt tcataccacg gctgtctttg aagacggatt gacagtcgca
    13501 gacccaacag gattgtttac cttaaacgat accgttgaaa ttaccgagat tcttccagga
    13561 aaagcatact tggtcaaagg gatcttaatg gataaagcaa ccggtgaacc cttgcttgtg
    13621 catggaaata cggtagaaac gagtgttggc tttattgcca aagcagaaaa tgaaacggtg
    13681 atcgtaccat ttacctttga tttgacaggt ataaatggtc gtgaaatcgt cgtgtttgaa
    13741 agtcttttta gaaatgatga agaactagca aaccataaag acatcaacga caaagggcaa
    13801 acggtacgag taactaaccc aagtgtacgt acaacggcta cacataaacc cactggcttg
    13861 aaagaggtta atccattaac gaaagtcacc attacagacg ctgtgaaata ccacgatttg
    13921 gtcgttggaa aaacatacac agtcaacggt gttctaatgg acaaagtaac ggaaaaacca
    13981 gtgctagtag atggaaaaga agtcacaagt accttaacgt ttgtggcaga aacaacggat
    14041 ggtacagtca atctagactt tattctagac gcacgtgcgt tgcgaggcaa agaagtcgtg
    14101 gtctttgaag atctgtttag agaaggcgta ttattggctt ctcacgcaga tattaccgac
    14161 aatgaccaaa ccatcaaaat cagtaatcca accattacga cgaaagcgac gaataaaaat
    14221 ggcggaaaag agatcttggc cttgccaaat caaactgtgc ttgaatgggt caaagtagac
    14281 ggtcttgcga ttggtcaaat ctatcgctta attgttcaag gctacttaac accagacggc
    14341 acaccatttg aaggcactca tgcggaaaaa gtctttgcgg ccgataaaga aatgatggaa
    14401 ttcttatttg aattcttagt ggacggtcgc aatcttgcag gaaaaggctt gagttttgcg
    14461 gaatggctcc aagcgaaaac ggaagatgag aaagaaacgt ttgaagaagt ggcgaaacac
    14521 aatgtagatc taacaaacaa agatcaaacg gttcaaattg tggaagcgcc aaaaccaccg
    14581 caaccagtga aaccagaaca acgggttaaa ccagctactg ttgtaccaat gttgccaaaa
    14641 acaggtgaac aaacaagtag tatacttatg gttcttggat tagtagtatt agtcattgct
    14701 ttaggtataa cactttataa agtacgtaaa gccaaagatt agtcatgagg aacaagcggt
    14761 taggattttt aaccgcttgt ttgattgtaa tgtagaaata tttagaattt attttggttt
    14821 ttaagaggag gaaaaacttt atggcagaaa aagcattgcg tatgaagaaa gagtttttag
    14881 gtcaagtatt tttattgggt tcacgggaac ctaatccacc aaaggaaatt aatggtgtgt
    14941 ctgggcgata tgttcaatta caagcagagt gttcgcgaga agaacagcct gctttttttg
    15001 tttatctcac agctgaagag gataaaagat tacgattacc agaaaaagag gtgcgtattt
    15061 catttgaaga agcttatttt gtttttcgta aagaacgagt gaatagtcgt gaaacaaatg
    15121 atatgggaca aacagagcgt cgaacaactg gctatcaaga tgttggtgtc tttttaactg
    15181 gtgtaaaaat tggggaggtt ttgtaagatg ggattaaaat ttccaaataa ttatgacatt
    15241 acagaagcat tagatgaaac ttgtttagga gccgtacgct ttggcgcatt agaagagtct
    15301 gatcgtgtat ttgataatga aacaggcgat tatacgggag aaattcgatc acagcgagca
    15361 caattagttt ttggaaatcc agatgaacca aagattgcac gacgagttaa atttccagca
    15421 gatgtagatt tgactggttt taaacgacgt gatcctgtga aattaattga tccagaatat
    15481 catgtggtat atattccagc acaagatggc aatcgtgaat attcagattt agtgatttat
    15541 gcgaaaggat tagagaaagt agaagctcca acaaataatc ctaatatgaa acaatcacaa
    15601 gaaaaaggtt tagatcaaaa acaaccaagt gttgattcta aggaaaaaga gaaaaaataa
    15661 ggtttttttg aaagaatagg aaggagtctt cctattcttt ttatctaaga tagaaggaag
    15721 gggagatttt gaattcagat aaaaccaatc aaatactttg agagattgtg cttgttatca
    15781 aaatatccaa caattgattg agtattttgg agacaatcca acgatttttg tacaaacaaa
    15841 tgcatatatt tgtgcggtta ttgggcagtg tatgttttta cgtgatcttg ttcgtttttc
    15901 attaccgtac cagtggaaac aagatcatca ggaagtgatt tggattcgta tagtacgaat
    15961 atgtaatatg tgtgcacacc cttataaaaa tcgattaaat ctttttcgat tatgtttttt
    16021 tactaaatgt gatattcctg tattagttgc tttttgtcaa aagtttttag cacaggaaaa
    16081 gtaaaaacag aaaggaggaa tgtgacaatt gaaagctcat tttttcaagt atcgtggtcg
    16141 gcgaattcgc tatttcaatc gttatttatg gttttgctat tatggtttct ttttgtttcc
    16201 atttttgatt ctgttaggtt ggtttagttg gaaagtattt tatccacgtt taagtacgtt
    16261 ttcgaattgg caaatgtatc ttattcctgg aataagtatt gtgttgtatt tgttgctggt
    16321 tagtgggtta attttagggt tgatgcattg gtcgcgacta aaagaaggat attttgcctc
    16381 tgtttattgg cgtcagttac ttgttcgaat gttaattgac aatcggtttg tttatacaaa
    16441 gaaacaagtt agtgagaaac aaacaaggga aaaaatgaga ttaccaaaag tttattttta
    16501 tcaaaagaaa caagaattag tggtttcttt tcctttagat ggtgggcgtt ttcacgaacg
    16561 atttttaaaa atgggtcatt tgttatctga agtatttttg cgtgatttaa ttcgtgaaga
    16621 acgagaaaaa gcccgaatca attatgtatt cctttcagat tctggtcgga ttcctattga
    16681 tcaatgtata gcagaaaatg gacgtattca tttaatgaaa actttggatt gggtatatga
    16741 tcaatcacca aatatgttga ttgctggtgc aatcggtggt gggaaaacct atttattgta
    16801 ttctttaatt caagcttgtt taagtgttgg gacagtagat atttgcgatg gaaaagcggc
    16861 agatttagca gcattaggtg acgtcgatgt attcaaagga catgtgtttt acaaaataaa
    16921 tgaagaaatg attatttgtt tacgaaatgc attaaaagaa atgaatcaac ggtatgccta
    16981 tatgaaaacc atgaaagatc ctaaatatga accagggaaa aattatgctt attatgggtt
    17041 accaccgcat tttatctttt ttgatgaatg ggcagctttt tatggttcgt tggatatgga
    17101 agcgagaaat agagtcgata aattgattca gccattagta ttaaagggtc gtcaagcagg
    17161 gatgtacatt attttagcaa tgcaacgacc agatgcagaa tattttccaa gtggtgttcg
    17221 ggataattta acattccgta tttctgttgg tcgattgtct cccattggtt atttaatgac
    17281 ttttggagaa gattttaaaa ataagccatt ttacaacaaa gatcaacctg gtcgtggata
    17341 tgcttttcca ggatttgatg tacctgtaga gttttttgcg ccatttgttt acaaagatta
    17401 tcactttatc aaagaatttg agaaatttac tgaaatgatt gtctatgatt ttacagtaaa
    17461 agaggatagt caagatcaaa cgatgggaga tgataatcag ttgttacaag aacaactaga
    17521 gcgccaaatg tatttagcaa aagatccaaa aggaacaagt ttttctgatg cgcatatttt
    17581 gtaatcaact aaggaggtga agttaaatgg cagagagttg ttattataga gtagctacag
    17641 caatacgaat gattaatcct tctctttctt ccagaacgtt ttatgattgg ttgaatcgta
    17701 ttgaacaagt aactgattac cgctttttgc gcaaagaacg tgtttttaca ggaaaagtta
    17761 ttaatcaagt attgctaacg aaaaaagata tagaacgatt gactcgattg tatcattatc
    17821 gagtagattt agaagaggat ttaactttgt caatttatcg cgttttttct cctgaaaaat
    17881 atagtgaaat aacaaaatta gatcatttaa ttttgtagtt attatctatc gggagggctc
    17941 tagacgtagt ctggtagccg cactctgcct ggcggtgcgc attaaggctg cgaagcatgg
    18001 gcctataatg cgcactcgct agcagcgtgt gcagctcaca aagaggtcgt gcgcttgcgc
    18061 acggcctctt tgtgtaaagc cccccgatat ataatatggg gggcagaaat aacataacac
    18121 atagaaaaaa gttttataaa cccttttgta tcaagtgatt atctaaggtc agcagttgta
    18181 ctacttttcc caaaaaacga gggggactag ttgatatatc ggtgtttcta aagggttaaa
    18241 aaaaatcgat tttgttattt tataaaagga gcgagaaaat gacaccgatt gagctaaaaa
    18301 gctacagaaa gaagttaagt gcaattcata atataagtgg agataaaaag ccgctatcac
    18361 aagcaagatt tgcgcaattg atgggctgct caaaagatta cattagaaag atggaacaag
    18421 ggaaaagaaa aatcccagag gattttgaac aacgaatcaa ggaacgcttt cgattgtata
    18481 tgaaaagcgg aatagatctt cgagcaatga ttgattattt gcgtctttcc ttttttggga
    18541 cagactacaa aacagtgatt caacgtgtat tacgaatgga tctgacagaa tttgaaacac
    18601 gtccaacggg cttgtatctt tttgactgtc tttctgttcg tgggaatatc tgggtgttct
    18661 ggcattcaaa acgaactgta acagaaaatg tacttgtgca attttcagga caaggttgcc
    18721 gggaatatga ggaagtcatg cgtgaacaac aaacagattg gcgaacatgg ctatattcca
    18781 tttggaaaaa tcaaggaaag ttggaagctg gttatacacg tgtgcaatgt agccgattcg
    18841 atattgcgtt agatgaactt tggggattgg aaacggaaga acatttcgat ctctttgaat
    18901 tactagaaaa acaaaaagca ggtttattag aaatggactt taagagattc cagaatattg
    18961 gcggttgttt tttagatgat ggctattttc gaagtgaagg attatctttg tattttggat
    19021 cgcgaaagag tcctctcttt tttaattttt atgagaaacg ttttgaaatt gcgaaccgag
    19081 aaaaaatttc agaattagaa gcattaacga aatatggtat ttataatcga tatgagttac
    19141 ggttagccaa tgagaaagca acacaagcag tagaagcatt tattttaggt gattcaccga
    19201 aacgtttagg agagattggc gttggtttaa ttaatgcaaa acttattgtt tgggatagac
    19261 tggaaagtga tgcacggatt gttgataaac gatgggcaaa attatttcgt tcgatggaag
    19321 aattggattt tacagtaaag ccagaaaaat tctcattaga tcgttcagaa cgctggtttt
    19381 tgaaacaagt agctccgaca ttaaagttgt tacaagaagc agatcgtatt ttaggaagag
    19441 actttgtaca agaagggatt gataatgccg aactaaatga cgagcaacaa aactatctag
    19501 aatggatggg aacgtcagga gggaaaattg catgaatgca tatacgacaa cagaatggct
    19561 gcgcctgttg gatttaaagg ctgcaatcga agcattaaat gaaaagatgg ttgatttgtc
    19621 ttattttcgt tttcgagttc cttatattga acaagcggta aaagctggac gttatcagga
    19681 aaaagagaac tggcaagaaa ttgcacgctt attagaagtt cgtaagggat acgaacaaga
    19741 actcgaagag ttggaattta gccgaaggaa aggaaggcta gaattcattc gcttttatcg
    19801 attttcattg ccaatacctg cgattttagc ggttaaaaaa ggctgcgata aaatgaagat
    19861 atatgaaaac tgtgtggcag cacttagcag cgaaaaacct cttatggaag aaatttcatt
    19921 ggtgactgtc acttgggaaa tgagtcgcca gccaacagaa gaacagacat atctttcttt
    19981 agaagagatt gagatagaat tagaggaaat tggtcgctat gccacttgtt ctacctattg
    20041 tggatcggtc attagtattg ctggtgtgat tgtctaaatg aaaaaaatta tgatttatgt
    20101 tggtgcgtat tggtcacgtg atccaacagt attagaaaat ccagaggcgg tacactattg
    20161 cttgcgccaa ttattctatc tgtataaaga acgattagaa tcgttgattc gtcagttacc
    20221 atatactgac agacgtttag atgaacttct tctaaggtat ccagcaatgt ataaacgacg
    20281 aaaaaataga ttgttaccag aagagtatcc cattgaaaaa catgaacttg aagggcgatt
    20341 tgtggcttat ttctatgatg atgttcgtat gcgcttagta gaacaacgaa tggaaataga
    20401 aaatgatcgc tactatttta tcaattattg taagaagcgt aagtatcaag taactgatga
    20461 tttttatgat tgtattttac aagatgggga agtgattcta tccaaaattg ctccaagttt
    20521 tcgtgaactt gtgccgattg attttttgaa aaaatgtcat ttgcggattc ttccgtaaaa
    20581 aagagctata aaaggaaaga aaagtagaag ccaaatcggt ttctgctttt ttattttttt
    20641 taggaaggag aagatggtat gtcactggaa tttttaggag agccaattga aaaggaagag
    20701 ttcgtttcgc attatatgga tttgtttatt caggtgataa aaaactttgt gcgattgatg
    20761 aattttatcc acgcgaacaa gactatctag ccgctgaacg aataaaaatc agtttattat
    20821 gggaaaaatt cacagatgaa aataaccgac cgccagatta tcgctattta tcagattgtt
    20881 taacgaataa tgtgctttct aaagaatacc tatttcacaa acgtgagaaa aatcaaatgt
    20941 cggttgaaca tttttgtgat tgttatattg cattattgcg tagtcaacag ttattaacag
    21001 tagaacgata tggtgcagag tttggttatg tcatgcaagc tgagcgtcat agagctacaa
    21061 gatgctatga aaaagaagat caagtgattg atggctatga agaattatgt attcgaaaca
    21121 atcgctattt gcaacaacgt ttatatgaac aattgatgat ggatttttca aatctctata
    21181 gagggtatga atgttcgatg attcaagaaa ggtagaagaa aagggggaat ttttatgtgg
    21241 cgattattat ttttggtggt atgtggtatg gcattgttct atgtgtatcg tgtctatctc
    21301 tatccacgaa tggaacgatg gacaagttgg tatagagatc ctttaaaaca acgaaaagga
    21361 ttgaagcggt atcgttatga attacttgtt gaattttcgg atgggcgaca agcacagtat
    21421 tatttttttg caaactatcc aaaatacaac accagtgaac tattgattca attattcaaa
    21481 aataatccat atgttgggtt agaagaaaac ggacgggagt tcttttatcg ttcagataat
    21541 attagtgcgg cgtctatgaa caaaacacga attgcataaa aaattggagg aataatcatg
    21601 aaaaaaacaa cattagcagt cgtaacaaca ctaggaattt cattattttc gtttcaatta
    21661 gggagttcat tggtttatgc agatgaaact gtagaaacac ctaagtcaga aattgtagaa
    21721 acattgccag gggaaggatt accagatgtt gagacagagc taaatggagg aacaacggaa
    21781 aaaccagcag aacctgaaca attgcagtca gagaaacctg aaaaagagcc agtgacacca
    21841 gaaccagagt ttcctgaatc aactttaccg actgattcga tcgaagaaga aggtgtgcat
    21901 agttcaacaa caacggattc aagtatagtc acaactccgg aacaatcagg aacaaccagt
    21961 tcttctgaaa agaatactga ttcgtcaagt acggaacagc caacgatgtc gtctaccagt
    22021 gaagaacaaa aagaagaacc gaaacaacct gttgaaaaat cagaagcaat tccaaaagaa
    22081 aataaaccta ttgttccagc aaaagaagtc acagtatcgg taa
//
LOCUS       DAZUKC010000046        20335 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000046 DAZUKC010000000
VERSION     DAZUKC010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.047"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            49..3438
                     /locus_tag="ACG80T_001660"
     CDS             49..3438
                     /locus_tag="ACG80T_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS4841300.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            3441..4862
                     /locus_tag="ACG80T_001661"
     CDS             3441..4862
                     /locus_tag="ACG80T_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS4841301.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            4859..6736
                     /locus_tag="ACG80T_001662"
     CDS             4859..6736
                     /locus_tag="ACG80T_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS4841302.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            6828..7670
                     /locus_tag="ACG80T_001663"
     CDS             6828..7670
                     /locus_tag="ACG80T_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS4841303.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(7822..8697)
                     /locus_tag="ACG80T_001664"
     CDS             complement(7822..8697)
                     /locus_tag="ACG80T_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4841304.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            8904..9935
                     /locus_tag="ACG80T_001665"
     CDS             8904..9935
                     /locus_tag="ACG80T_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4841305.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            10024..10329
                     /locus_tag="ACG80T_001666"
     CDS             10024..10329
                     /locus_tag="ACG80T_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFS4841306.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            10508..12523
                     /locus_tag="ACG80T_001667"
     CDS             10508..12523
                     /locus_tag="ACG80T_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS4841307.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            12525..12809
                     /locus_tag="ACG80T_001668"
     CDS             12525..12809
                     /locus_tag="ACG80T_001668"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4841308.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            12835..14208
                     /locus_tag="ACG80T_001669"
     CDS             12835..14208
                     /locus_tag="ACG80T_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFS4841309.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(14291..16306)
                     /locus_tag="ACG80T_001670"
     CDS             complement(14291..16306)
                     /locus_tag="ACG80T_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFS4841310.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16328..17542)
                     /locus_tag="ACG80T_001671"
     CDS             complement(16328..17542)
                     /locus_tag="ACG80T_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4841311.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            17763..18707
                     /locus_tag="ACG80T_001672"
     CDS             17763..18707
                     /locus_tag="ACG80T_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS4841312.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            19003..19947
                     /locus_tag="ACG80T_001673"
     CDS             19003..19947
                     /locus_tag="ACG80T_001673"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFS4841313.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT         6950 a         3727 c         3872 g         5786 t
ORIGIN      
        1 ttttcactat tcacgtcaaa acagaaaaaa agaaaaagga ggggaaaaat gacgaccaca
       61 gggaagaaac tgaaagttat tttcatgctg ataatattga gtttatcaaa ctttgtgcca
      121 ttatctgcaa tagcagacac tacagatgat ccaacagttt tagaaacaat ttcagctgaa
      181 gtcatttcgg atcagtctgg aaaaaaagca ctgaacatca agctaaatgc gaataacacc
      241 agtgctgaaa agatagaaaa agaaattggt ctagtcgaaa attacttaag tgatgtggaa
      301 agaaaagaag gagatggcta tgcttatcag gtaaatagcg ggaaaattac gttggaaatc
      361 tcatcaaaca ctaaacaaac tatcgatctg agttttccaa tcgatccagc actttaccac
      421 agccaggcaa acaagctgat cgtcgataat aaagaatatg acattattga tgagacagaa
      481 aataagaaag atacagatgt gtcagtacca aagccagacg aaatagaaga agaatcatca
      541 aaagaaaacg aaaattctgt cagcccattt acattgccta cattatcctt gccagctgtg
      601 agtgtgccat ctaatcaaac gattcctaca gaatatacaa cagatgatca gggcacttat
      661 cctaaagcca gttggcaacc tacaggaaat acaaatgttc ttgatcatca aggcaataaa
      721 aacggaacaa atcaatggga tggtataaat tcttggaatg gagatcctaa tgatcggacc
      781 cattcgtata tcgaatatgg aggaaccggt aatcaagcag actatgcgat acgaaagtat
      841 gcaaaagaaa caagtacacc cggattgttt gatgtttatt tgaatgctcg tggaaatgta
      901 caaaaagata tcacgcctct tgatctcgta ttggtcgtag actggtcagg aagtatgaac
      961 gacaataatc ggatcggtga agtaaagatt ggtgtcgatc gttttgtcga tactttagca
     1021 gatagcggta tcacagacaa aatcaatatg ggatatgtcg gctactcaag cgaaggatat
     1081 agctacagta acggtgcagt acagatgggt tcatttgatt cagtgaaaaa tcaagtaaaa
     1141 tccattacac cttcacggac aaatggtggt acttttacac aaaaagcact aagagatgca
     1201 ggaagcatgc tatccgttcc aaatggacat aaaaaagtga tcgttttgct gacggatggt
     1261 gtaccaacat tttcctataa agtacagcgg gtacacgcac aatcaagcag caattattac
     1321 ggaactcagt tttctaatac gcaagatcgg ccgggaaata cttctctaat ctcaagaatc
     1381 tatgatgcac ctgaccaaaa caatctatcc agaagaatcg acagtacgtt tatcgcaacc
     1441 atcggagaag cgatggcact caaagaacga ggaatcgaaa tacatggtct tggcatccaa
     1501 cttcaaagcg atccggcagc tggtctctca aaagcagaag tagagtctcg tatgcgacaa
     1561 atggtttcat cagatgaaaa aggcgatctt tactatgaat cagctgatca tgcaacagat
     1621 atctctgaat acctagccaa aaaagctgta cagatctcag caactgtaag caatggacaa
     1681 ataaatgatc caatcgcaga accattcatt tatcagcctg gtacactttc agtcaagagt
     1741 gtggggacaa gtcctacaac ggtcactcca tctatttcca tagaaggaaa taccatcaag
     1801 agcaatcaga tctatttagg aaaagaccaa gaaatccaaa tccattacca agtgagaatc
     1861 caaacagaaa atgaggactt ccatccaaat ttctggtatc aaatgaacgg caggacaact
     1921 ttccagccaa acattgatac caatgaatta gctgaattcg gtataccatc tgctaaagct
     1981 cccggagtca gtcttcacat caaaaagtta tgggaagaat ttgacaacaa tctagctgat
     2041 cgtccagatc aagttacttt tgagattcaa cgggaacata cgacaaatgc tgcagcttgg
     2101 aaaaacggat atattcgaat cattaaacca gctaaagata caacaaatac gtgggaacgt
     2161 gcagacattg acaaattatc tgcaaatagc ggagaaagtt atcaagagat attatcacta
     2221 cctcaataca ataatcaagg tcaagcattc agttaccaaa caatcaaaga attacctgta
     2281 ccaggatacg attctcaaca aatagatgca atgacatgga aaaatactaa acaattcaca
     2341 ccgttaaact tgaaaataac gaaaaattcc tctacaggtg aaaaggatct tattggcgct
     2401 gttttcaaat taacaggaga ttctattgat actttactaa cagatcatgg cgacggaacc
     2461 tattctcttc cagaaaatgt caaattgcaa aaagaaatga cctatacgct gacagaaaca
     2521 aaagctccag aagggcatgg attaagcaaa aagactactt gggaaatcaa gatcgcttct
     2581 gatggtacgg taaccattga tggaaaaaca gtcactactt ccgatgatac gatccagttg
     2641 actattgaaa atccttttgt tgaagttcct gtagcagtac gtaagtatgc gatgcaaggg
     2701 acggacaaag agataaatct taaaggagca gcattttccc tacagaaaaa agaagcaaat
     2761 ggtacttatc agccaattga cagccaaaca acgaatgaaa aaggtcttgc cagttttgat
     2821 tcactcacac ctggtaaata tcgagtcgtt gaaacagctg gtcctgccgg atatgatact
     2881 tcgccgggaa attatgaatt ccaaatcgat aaatatggaa aaatcattta cacgggaaaa
     2941 aataccgaga tgacaaataa tgtatggacg ctcactcatc aaaatcgact aaaagcgttt
     3001 gatctaacgg tacacaaaaa agaagacaac ggacagacat taaaaggagc aaaattcaga
     3061 ctgcagggac cagaaatgga cttagaatcg ccaaaagatg gacaagaaac agataccttt
     3121 ctattcgaaa atttaaaacc tggaacttat acgctgaccg aaacttttac accagaagga
     3181 taccaaggtc taaaagagcc agttactata gttatacacg aagatgggtc aattcaagtg
     3241 gatggacaag atcatgaatc tgttctgtca ccaggagcca aaaacaacca gatttcttta
     3301 gacatcacga atcaggcaaa agtaccatta cctgaaacgg gaggaattgg ccgtttagga
     3361 atctatctag tagggatgat tggttgtgcg ttttctattt ggtatctttt tttgaaaaaa
     3421 gaaagagggg gcagctaaat atgaaaaaac ttggttggct tagtatgtgt ctcttcttgt
     3481 tactatttaa accagctttt actcaggtag caacagaaac agaaacagaa atggttcaga
     3541 ttactttaca caaattgctt ttcccaaacg ggcaactgcc gaaaaatcat ccaaatgacg
     3601 gacaagaaaa agctttatta caaacgtatc gaggattaaa tggtgtcaca ttccaagttt
     3661 atgatgtcac agattctttt taccatctac gggaaaaggg caaaacggta gaagaagcac
     3721 aagcagagat cgcaaaaaac ggtgcgtctt ccggtatgtt taccgcagaa gcaacaacta
     3781 caactcttaa caacgaagat ggtatcgctt ctttttctct ggccgctaaa gatcaagaaa
     3841 aaagagataa agcgtatctt ttcattgaat ccaaagtacc agaagtcgtc aaagaaaagg
     3901 cagagaatat ggtagttgtt cttcctgtac atggacaaaa caatcaaaaa ctttcaacta
     3961 tccatttgta tcctaaaaat gaagaaaacg actaccctga tccacctttt gagaaggtat
     4021 tagaagagcc tagaaatgat tttacgattg gtgaaaaaat cacttattcc ttgcatacga
     4081 caattcctgt aaatatcctt gactatcaaa agttcgaatt gtcagatagt gcggatgaag
     4141 cattaacgtt tttacctaat agtttaacga tttcatcgaa tggagaaaag ctgacagaag
     4201 gctttgtcat acacaagaaa cctcacggat ttgatgtttt attttcgatc ccttcgttgg
     4261 aaaaatatgc tggaaaaaaa ctgaccattt cttatcagat gcagctaagc agtacagcac
     4321 aggcgaacaa ggaaatcaac aacaacggaa cactggattt tggttttggt gtcagtacaa
     4381 agaaagtctc tgtatataca gggagtaagc aatttgtcaa aatcgagaca aataaaccag
     4441 ataaacgatt agctggcgca gtattcctta ttaaaaacaa agcaggaaat tacctccagc
     4501 aaacagccaa cggatacaag tggacaaaga acgaatcaga tgcgcttcac ctgatttccg
     4561 ataaaaatgg cgctttttca atttccgggt tgaaaacagg aagttatcga ttaaaagaga
     4621 tcgaagcacc ttctggttat attttaagtg aaacagaaat tccgtttacc atttcaactt
     4681 ttctttctga ggataaagag gcggacagta tattgaaagt agtcaataaa aaagaaaata
     4741 gccgtccatt tcttccaaaa acaaacgaaa cgaaaaatac acttttaggc gttgttggta
     4801 tggtattcgc aagctttgca atctggttgt ttatcaaaaa aagaacagga gtgaaaaaat
     4861 gaaaaatcat aaaaaaataa acgttatgtt aggagtcctt ttccttattt taccattact
     4921 cacaaacagc ttcggcgcaa aaaaagtgtt tgcagaggag acagcagctc aagtcatcct
     4981 tcataaaaag aaaatgactg atttacccga tcctttaatc caaaacagcg ggaaagaaat
     5041 gagcgaattc gatcaatacc aaggattagc cgatatttca ttttcagttt ataacgtcac
     5101 tcaagaattt tatgcgcaac gagataaagg agcgtccgtg gatgcagcaa aacaagcagt
     5161 ccagtctttg actcctggta caccagttgc ttcaggaacg acagatgctg atggaaatgt
     5221 cactttatct ttacctaaaa aacaaaatgg gaaagatgca gtctacacga tcaaagaaga
     5281 accaaaagac ggagtgtcag ctgccgcaaa catggtttta gctttccctg tatatgagat
     5341 gatcaaacaa gcagatggct cttataaata cgggacagaa gaactagata ctatccatct
     5401 ctaccctaaa aatacagtcg gtaatgatgg aacgttgaaa gttacaaaaa tcggtactgc
     5461 cgaaaacgaa gcactaaatg gagcagaatt tattatttct aaagaagaag gaacaccaag
     5521 cgtcaaaaaa tacatccaaa gtgtcacaga tggattgtac acttggacaa ctgatcaaac
     5581 caaagccaaa catttcatta ctggtcattc ttatgacatc ggcaacaatg actttgccga
     5641 ggcatctatt gaaaaaggcc agttgatcgt taatcattta gaagttggaa aatataattt
     5701 agaagaagta aaagctcctg ataatgcgga aatgattgaa aagcaaacaa tcacgccttt
     5761 tgagatcctg gcaaatagcc aaacaccagt agaaaagacc atcaaaaatg atacgtctaa
     5821 agttgataaa acaacacctc aattgaatgg aaaagatgtc gcaatcggtg aaaaaattca
     5881 atatgagatt tctgtcaata tcccattagg tatcgctgat aaagaaggaa cgcaaaacaa
     5941 gtacacaaca ttcaaactta tcgatactca tgacgctgct ttaacatttg ataatgattc
     6001 ttcaggaacg tatgcttatg ccttatatga tggaaataaa gaaatcgacc cagtaaatta
     6061 ttctgtcact gagcaaacag acggattcac ggtttcagtt gatccgaatt atattccttc
     6121 attaactcct ggcggtacat tgaaattcgt ttactatatg catttgaacg aaaaagcaga
     6181 tccaaccaaa ggattttcta accaagcaaa tgtcgataac gggcatacaa atgatcaaac
     6241 accaccgtca gtcgatgtcg ttactggggg caaacgattt gttaaagtag atggtgacgt
     6301 tacatcagac caaacacttg ctggagcaga attcgtcgtt cgtgatcaag atagtgacac
     6361 agcgaaatat ttatcgatcg acccatccac aaaagccgtc agctgggtat cggcgaaaga
     6421 atcagcaacg gtttttacaa ccacaagtaa cggtttaatc gatgtgacag gtctaaaata
     6481 tggcacgtac tatctggaag aaacgaaagc gccagaaaaa tatgttccat taacaaaccg
     6541 tgtagcattt actatcgatg aacaatctta tgtaacagca ggacagttga tttctcctga
     6601 aaaaatacca aataaacaca aaggtacact tccttcaaca ggcggtaagg gaatctatgt
     6661 gtatatcggt gcaggagtag tccttctact gattgctgga ctgtactttg ctagacgcaa
     6721 gcacagtcag atttagtctt tttgcaagaa ttggaaaaag gagcagcgga ttacgcaaat
     6781 gctccttttt cttttattat caaacagtca tcacaaggag aacacaaatg aaaccaaaga
     6841 aaaggaaact tctagatctg ctgatgcttt tcttgcttct ctcaggaata gggatactag
     6901 cttatccatt tgttagcgac gcgctgaata attatctgga tcagcagatc atctctcatt
     6961 atcagcaaca agcggtcaaa gaaaatgaag aagtcatggc taaaatccaa gaaaacatga
     7021 caaagaaaaa ccagcaatta gcaaaagaag gtggaaatcc aggtgcagat ccctttacta
     7081 agaaaaaaga acctgcgaaa aaggatagaa cctattttga gaaacatacg atcggtattt
     7141 tgacgatccc tgagatcaat gtccatttgc caatttttga tgaaacgaat actgtgctgc
     7201 tagaaaaagg tgcctcatta ttggaaggaa cgtcttatcc tacgggggga gaaaatacac
     7261 atgccgttat ttccagccat cgaggactgc ctcaagcaaa gttgttcaca gacttaccga
     7321 aactgacttt aggtgattct ttctatatcg aaatcaatgg acggacactc gcttatcaag
     7381 tcgatcagat caagacggta gaaccgactg atacaaaaga tctgagaatc gaaaaagggc
     7441 aggatttcgt gacactccta acttgtacac cttatatggt caacagtcat cgcttacttg
     7501 tcagagggca ccgggttcct tatcatgcaa aagaagttca aaaagaagta cagaaagtat
     7561 ccgagaacaa acgatttttc ctgtatggat tgttagctgc atgtatactg attttcctcc
     7621 ttcttatata tctttttcgt caatcgttca aaataagaaa cagaagttag ctattcctca
     7681 tttctataat atatctattc gaatcgttcc gtaaacaaac aggctgaggt ttgaaaaaga
     7741 aaacttcagc ctgtttttat gatgatatca aagagagcct atcaaacact atctttctta
     7801 ttactaaatg aggatctttt cttaagtttt ccgatattct ttaagaaggc ctttttgcat
     7861 caattcttgt ttgtttttca agttgcggat attctcttca aaattttgag aatatatcac
     7921 tgagaataac gtatttaata cgtaatcgaa ggcaatttga gaggagaaag tcgctacttt
     7981 agcaaaatca tattcctcct gcgatataac aagacacata tcagcctgtt tggccatttc
     8041 tgaatgctga tttccggtaa tcagcaaggt aggggcctct acatgcttca aataggtcat
     8101 tattcgttca taatgatgga tgcgaccgct gtagctaata aaaatggcgc aatcctcgct
     8161 agtcaaattg gctgcattcc aagaagaatc actgtattct tcagccatga tcaaaaactt
     8221 attcaacttg accattttat tctggaattt ccttgccgtg atttgagaat caccttttgc
     8281 aaaaagaaag attcgctgtg ccttgttcaa tactttaccg atttgatcta aatcttcatg
     8341 ttcaatctgc aattgtgctt tttttattga ctggatcgat aaatccgcca ttttctttgc
     8401 aatttgctga gtgctatctt cacttgaaaa aggaaaatta ggatcaatcg tttgattgaa
     8461 ctgagcaatc aattgatgaa cttcccgccc cagctcgaaa cggaattctt taaatccgct
     8521 gaatcccatt tttttgcata aacgaatgat agctgaatgg gaagtatacg tagcttttgc
     8581 taattcttca atcgtcatgt agactgcagc ttcgatattc ctacggatgt aatcgacaat
     8641 ccgtttctct gtcgtggtaa acaactcttg ttttgttagt ttttcttcaa taagcatgat
     8701 ctgacctcca aattctatct tactgataaa cacttggttc cgctactatc tttttgattt
     8761 ttatccttac tttgaacatt aatttcacca aaccgaatga taacaagtgt ttttctgaac
     8821 ttttttacca gaatagctat tataaatact ttctgcgctt ttctaggata tactgaaaac
     8881 atcaataaaa ggaggcacca ctcatgctta caatcgctta tcttggcaat ggaaagagta
     8941 caaatcgcta tcatttgcct ttttcaagaa aacttacgaa caaaatcaat atcaaaaaaa
     9001 ttttttcccc atctgaacca gtgtggaaaa cattcgaaga catcgaatat actgatcagc
     9061 tgtctgatct ttgggatgat cctgagatcc aattagttgt catcactaca cctagtcaat
     9121 ttcattatga atatggaaaa atggcactcg aacatggtaa gaatgtacta gtagagaaac
     9181 cttttgcaga aacgtcaaag gaagcagaag agttatttgc attagcaaaa gaaaaaggtc
     9241 tatttatcca atgttaccaa aacagacggt tcgattctga cttcttgaca gtccaaaaag
     9301 taatcgaaag cggaaaatta ggggatattt tagaagtaga aatgcattat gattatttca
     9361 gaccggaagt accagaaaat acgcatgaat tttctactgc gaacagctat ttatatggac
     9421 atgcttgtca tacagtagat caagttcttt cttatttcgg cgaaccagat gatatcagct
     9481 atgatgtacg tcaactttta gggaaaggac gaatgaatga ttacttcgat ctggattttt
     9541 tctatggaaa tatgaaaatt tcgataaaat ccagttattt ccgcatcaaa gaacgtccaa
     9601 gtttcgtcgt ttacggaaaa aaagggatgt tcgtgaaaca aacaaaagat cgacaagaag
     9661 aacatctgaa aatgttttac atgcctgatc atgaagactt tggtatcgat ttgcccgaac
     9721 attatggtgt attgacctat atagatgaac aagggagtta tcatgaagaa aaagtaattt
     9781 ccgaggttgg tgattacagc cgtgtctacg aagggattta cgagacatta gttaatggcg
     9841 cagaaaaagt catcaaagat gaagaaacaa tcttacaaat gaaaattttg gaagaaggaa
     9901 tcaaaagcat tcaagaatca gaaggaaaaa tatgacatat ttgtaagaat cgggtttgtt
     9961 ttttgacgca taaactgtct tattgtacga tgaaaaagta tagaactact aggaggaacc
    10021 aacatgacaa attatgacgg tgaaaaacac aaacgattaa gaggttcaat ggcccctaaa
    10081 acagaagaag aagtggcttt agcaggaact aattctggtg aaacagaaga caaagaagaa
    10141 tcattagctg gcacaaactc tggtgaaaca gaaaataaag aaacagcttt atctggttca
    10201 aattctgtag aagaggaaac aaagcctaat gagcccaatc actttgaaaa agatatcgaa
    10261 aaagcggttg aagatgtcaa agaaaaattt gaagaactga cctcaaaaaa taaacatgat
    10321 catcattgat cggacgaaat caattcataa aagcaggaga catttggtca cctgcttttt
    10381 ttctgtatcc attcgataga aatcgttcgg tacagtcgta aaaaaaatcc aactgtaagt
    10441 tgtaccaaac acccctcgat ttaaaagcgc taccatacta gaatataagt aggaggtgaa
    10501 cagcgctgtg aactacaaaa ttagttatct cctgaaaaca ccaagagaaa tcagtctaga
    10561 aaaagcagca gagatcatcg aagtaaatac aagcgaggcg atcaaacaat taactccttt
    10621 tgccgaatat gtggatgata cccgtatccg tataaagaaa ttccgagcaa tcgactgggc
    10681 agaaaaattt ttggatatta ctcgatttga agctgtgtat accgaacaag agcgaagaga
    10741 tcttctttat ttgatggttt attcgactgc aacgtctgtt tctgtttttc attttcaaga
    10801 ttttcttcag gtttctaaag ggacagtcct gtcagatatc aaaaagataa gaaatgaatt
    10861 agaagtggaa gattgtcagc tgtcgtatac taggaaaact ggttttaggc tgctagggtc
    10921 agaagcaata ttaagaagag aagcaaaaaa tcgcactgct cgtttgatgc agtcacttgc
    10981 tggaagattt gggctcacgt atttaaccgc aaccctttcc attgattatt acgccaaagt
    11041 tagagatttg ctagataaaa cgatttcact atcagatctt cgattcgcac caaatcgaat
    11101 ggatgaatta gcttattatt tgagtttgac aaccattcga attggaaaaa catccatgga
    11161 actggtaaaa gatgctgaac tgttggcttc tcttgcagtc actgacatta gtcgacaagt
    11221 attgactggc cttggctatt ccgaacatca aaattcggag attatatttt tctctctttg
    11281 cttagcatcg atcgttgaag gagatgtcca ggagcctgca cttgattttt tgctggaatg
    11341 ttcttcagaa gtcatccatc gcatggaatt cctgatggcc gtagaatttg cttcgtttcg
    11401 tgaattattg atgaatgtct tttatcactt ggtccctgcc tattttcgta tttcctattc
    11461 tttttattta cctaatgtga tgatcgatca aattaagcat caatatgctt ccatctacga
    11521 aatgacaaga aaagcattac ttccgttaga aagacgaata gggaagagca tccctgaaga
    11581 agagattggt tttttcacta tactatttgg cggagagatc agaaaagcgg acgcagaaga
    11641 aagaaataga aaaataagag ccgtgatcgt ctgtccaagt ggcattagct cttcattgat
    11701 cttaaaaagt gaacttcagc aattattccc gatgatcttt tttacagaga ctaattcagc
    11761 tgaccgctta cacgaggttg acgaaacttc ttacgatatt attttttcta ctgtacctct
    11821 cacacttact gataagaaaa agtctctcta tgttctgcat ccaatcatga ctgacttgga
    11881 aaaaaatcag ctgatcaatc aagtacaaag agattggctg ataccaggat tttcaatgcc
    11941 ttctgcccaa gaattgttgt cggcgctact gccttatatc gaattaaaag aaggcgtaga
    12001 cgaagaaaag ctctatcaag tattaaatcg taaaatgaac aaacttttag aaaagaagga
    12061 ggatcaacgt cctatgctat ctgaattatt aacagaagaa atgatccaat tatcggatca
    12121 accaaaaaac tggcaagaag caatcacatt tgccgcacag ccgctattat cagaaaataa
    12181 aatagaagaa agttatatca aagctatgat cgagcgcgtg gagcagtatg gtgcgtttat
    12241 ccatattggc gatcacatcg cattacctca tgctcgtcca gaagatggcg taaacgaagt
    12301 agggatgtct ttgctgaaac ttcaacaacc tgtggatctg cttgataatc cagcccatcc
    12361 cattagtttg ttcatctgtt tagctgctat cgataatgag gcacatcttc gggctttggc
    12421 aaatttaaca aagctacttt ctaataaaca aaatcttcaa gatttattac acgcatcaac
    12481 aaaagaagaa atattagcga tcataagaaa aggagacgaa tgatatgaaa atttttgcag
    12541 tatgtcaaag tggattagga acaagtttta tggtagagat gaacatcaaa caagcacttg
    12601 cagatcttgg agcagatgtg gacgagtttg aagtaagtca tacggatgtt ggaagtgtat
    12661 ctagcgatat ggctgattat ttcttcattg agcattcttt gaaagatacg ctaacttcta
    12721 tacctgatga aaagctcgta cctttgcaat cgatcattga cagcgaagaa gtaaaagaaa
    12781 aagtaacgaa gatcttgtca aaagaataaa aatgattcaa gggaggaact atcaatggat
    12841 aaattgttga atttgtttgt gtcaatcgtc agtcagccct ccatcctcgt ttctttgatt
    12901 gcactgcttg gtctcgttct tcagaagaag aaattttcag aagtgatcca aggaactatc
    12961 aaaacatttg tcggattcct agtactgatc ggcggtgctg gtttaatcag taattcacta
    13021 accccttttg cttcgatgtt ccaaacagct ctgcatactc aaggagtagt tcctagcaat
    13081 gaagcagtag tggcgattgc tttgcaacaa tacggtacac ctactgcttt gatcatgcta
    13141 gtagggatga tcgtgaatat tttacttgct agattcacaa gatataaatt catttttctt
    13201 acaggtcaag caatgcttta tgtttcttgt ctaacagcag tcattttagt aagcgcgggg
    13261 tttactgcaa atttgtggat gatcttatta ggcggggtct tcgaaggaac actattaact
    13321 attacgcctg ctctttgtca accgtttatg aagaacatca ctggaaacaa tcaagttgcg
    13381 ttagggcata ccggaaatat cggttatgct tctagtggat ggatcggaaa gttttttgga
    13441 aacaaagacc gttctacaga ggacataaag attcctaaaa gtttcagctt tttgcgagat
    13501 tctactgtat cgatcatgct cttgatgtcg atcgtatatg tcgcacttgc tttgttagct
    13561 ggtcctggat ttgttgaaag agaactaagc aatggagaca acgctgtaat ctttgcactg
    13621 atccaagctg gaacattcac agctgggttt gttatcgtcc ttcaaggggt tcgaatggtg
    13681 ttggcagaaa tcgttccagc attccaaggg attgctaaaa aactcgtacc cgattctaaa
    13741 ccagcattag atgttcctat cgtttttcca tatgcaccta atgctgtatt aattggcttt
    13801 tttgttagct tctttgtagg aacactatcc atgtttgcta tgataggctt gcaaactaca
    13861 gttatcattc ctggggtagt aggacatttc ttctgcggag ctgcttctgg tgtcttcggc
    13921 aactcgactg gcggtagaag aggggcggta ttaggttctg ctttcaatag cttattgatt
    13981 agttggctgc cattatttat ccttccagta ttaggagatc ttaaaatcgc ttcttctaca
    14041 tttgccgata ctgattatct agtaccagga cttgtacttg ggaaactagg agaatacggt
    14101 caaatagcct tgacagttgg aatcatcgct ttctttttac ttgtcgtatt atctagcttt
    14161 ctttttgccg gaaaaagaac aaacattgtt tctaaagaac agaattaagc aaacttcaaa
    14221 ataaaaattg cttggacaat atcagaagaa gatttaaagt cttctgatat gtcgcaagca
    14281 gttttttgtt ttatgcattt atttttgaat agcttgttaa gacaagtggc tgttttgcta
    14341 gaagaggata acttttacca ttcattttca ctgttccgcc tgttctatca gaagtaatca
    14401 cgatcttatc ttttgttttt tcaagcgaaa tctttccatc tttgataggt agcgtacaat
    14461 tcagtgtttc aaatgaatct agattaggtt gtatcataaa ttcttcataa cctggctttg
    14521 ttggatacaa accgataaaa tagcgtccta aaagatagat tgggcttgct ccccatgcat
    14581 ggcataagct ttttccataa ggatctccat acatactgta cttttcattt cctgtttgag
    14641 tagggtcgta ttcttcccag aaagttaccg catcatgaga taacatccct ccccaataat
    14701 caagaatcgt ttggtagacg atattcgtct gtccgagttt gcataaagca tcttgttcat
    14761 agaatttgaa atatggagta gtaatctgtg tgatagtgtc attcaataag acattcttta
    14821 agatcgattg ctgttggtct ttgttagcaa aatcgaataa aacagcgaaa atattcgcat
    14881 gacgcgtcac atttcttctc ccagattcat agcaatcgat aaaagcgcca agttcttcat
    14941 cccaaaagta gtcaaatagg ttttgtttca atagttgata tttttcttga tagagatgag
    15001 tatgataatt ttcgttctct ccatatgttt cagctaagat atctccgcaa gtgatcatgg
    15061 aatgataggc tttcagtaat aagacttgtt ctgcagcaag cgttccttct ttatccattt
    15121 cagaccaatc aataaatatc caatcttttg ctctcccata aataaaccct aattcattcg
    15181 tttgctccat caagtatctc attaatgatt gcatcttagg gtagacgatg cgtaaaaatt
    15241 catagtcttt cgtcatcata tagtggtttt cgataccaat caaccacaag atcgaatagt
    15301 caacgatagt attcatatgc tgcttgattt cattttgtcc tcttaatgct aatatcgttc
    15361 gtttattgat ttcctcgtca aaaaaaagat attgattgat catgaatgat tgatacgcat
    15421 ctccagacca aatccagcga tctcgtttga taccgtcgat aaaaaacaaa tcactacata
    15481 acttgaaggc ttcttctgat actgaccaga tctgattcag taaagaagaa tcgcttgaaa
    15541 aagcagcttg tttcgaaaga gataaaaatt catgaaaaac ttgaagttga accattccag
    15601 tagaaacctc tggtataaac aaatagcgaa aagcgcgttt acgagctttc atttttcttg
    15661 tcattcctat ttgcttatag tagcaataat cgatatccat agcttctaca tcggactctc
    15721 cataacatag agtgatagat tcgatatggt catccagaga tattagttct acacagccat
    15781 tcactgctcg cccaaaatcg aataagattc ctccgccaat ctccgtttca gtgactggat
    15841 ggacgcattc tttttgataa tagatttgat ttggatcttt tgcgatatcg acataaagcg
    15901 gactccagcc agctggatat tcttcaataa aattacttgc tgtccagcca atatctgatt
    15961 ttacttcgtc tccctcgata aacatcgctg gtagccctga tgtatgtcct acaaagatac
    16021 gaatttttgc tttaccagct ggacaagtca gccattttcc acatggatgt ttttgtccat
    16081 taatttctac atacccaact ccttggatcg tcactttaaa catggaaggt tgatcaagaa
    16141 agtaatatct cttaaacttg acgttccgat ggcaatcatc catgtaccag tacgctggcc
    16201 agtcaaaacc tcgttcttcc cgttggaaat tttgtagcag tccatggcgg atctcaaagt
    16261 ctccaggata ccaaagccat ctatctttat gtttttcatt tgtcatttta cacgacctcc
    16321 atcaaattta ttttgttgtc aattgttctt ttatttcttc atctactctt atcactgtct
    16381 ttctattcgt cctgtgtact actagaaggc taataagcgc gctaatcaac agcaagatac
    16441 tgccattgac gaaaacagca agtgttttac tcccaagaga agcaaaagag agaaagagca
    16501 aaccatatgg tgcaatgaaa gtacctaaat tagcgccaac caataagatt gaagtattga
    16561 aactctccgc gtacttgggc gcttggatac ttacttggtt gaataagtaa ggaatgaata
    16621 acgaaaaaga aattcctgtt aggatcgcac caaacccaat cacatacagg tttatcacag
    16681 tggcaatcaa tacaaagccg attcccatca gtagtaaaga aagtgtaagc atatagcgat
    16741 gtgcttttgt ctgaagccag ccaaaacaaa ctccggcagc catacttgcc aattgcatga
    16801 ttgttaagat attcgctgca ttagtttcgt ttcctagaga ataagtcgtc aggactgtgg
    16861 ctaatttaat cgatacagca ttatatacca cgatgaccag caagagaaaa ataccataac
    16921 taatcatttt aggattcgtt ttgattactt gtcttgttgg ggctgtgcca tgcgattgaa
    16981 gttcagttgt ttcaggtgta ggaacaaaca aaacaaacaa gacaagaata ggtaagctga
    17041 tggcgtagac taaaaaggta tattgccaac cgaactgtac tagctgtcct gcaacaaatg
    17101 tcattaaagc tgcaccaaat ccttgaaatg cactttgaaa accaatcata gaggctcgtt
    17161 catctccgtg ataaaaggca gcaatcatgc tgactgccaa agaattgatc aatccaaagc
    17221 caatacctag tccagctctt gaagctaaga caatactgta actttgtgag aagaggggga
    17281 ggataccaca aatcgatgca atcactaatc cgatgatcac agtttgtttt tgtccgattt
    17341 tcctagcaat aaaactacta atcaaaacaa aaatagtgac cataaaagag ggagcagtcg
    17401 ttaataattc aacagcagat aaagaacgcg tggcataagt agctgacata ctgggaattg
    17461 cagacgtgac tgcaccagca gaagtcaaaa taagagagac tgacagcaga gaaaatttca
    17521 tcatcttttt attcataagc atgttccttt catttttgta agcgcttatc aaaaatatag
    17581 tatgaagtgg ttattctctc aatagcggaa tcacaggaag ttcatgtccc aaccatagaa
    17641 agtctttatg aaaccaaaaa tagctttgca tataatagca gtacagcgtg aactaataag
    17701 cgaaagaaac cgcttacaaa aaggagggag tcaattggaa ctagctgaat tagacatttt
    17761 ttttgcgtaa cgaagaatca atcgaaactc tgcagcgtga acaaggaatg aatatcaatg
    17821 atttaaactt agcttaccat cttcagacac cgccaaaaat ccaccgaggg gatctatttc
    17881 aacagggaaa tattcatatc agtaaacacc gtcgatatgc ggaagttccc gagcatacac
    17941 atgattttgt tgaaatcaat tatatgtata gcggtacatg tttacaaatt attaatggag
    18001 aaaggataga gatggaagaa agtgacgtgg tgatcattga taaagaagct actcaaagaa
    18061 tcgaatacac agggcaagag gatattttaa tcaacatctt attaaaagaa gaaaccttat
    18121 ctacagagat attgaatcat ttggcaagca caaataatct actttcttct tttttgataa
    18181 atgcatcaaa agaaaaaggg gaacatacac aattcattca ttttcgcggc ggaaaaacaa
    18241 gtcgtttgac gcatttgatt gaaaatatgc tgttgattta ttttgataca tacagtcata
    18301 agatccgttc gttaaatctc tatacatcac tattactgat cgaattgact catttattag
    18361 aacaacaaag cttttcatca gcagatggat gcgcagaaaa tgagatgctt agagtattag
    18421 attatatcga taagcatttt acttcttgta cgttaaaaga aacagcagct cattttggct
    18481 acaatccggt ctatcttagt aataaattga aaatgtattt tgatgcttcc tttcaagaac
    18541 tggttctaaa gaaaaaattc gactttgcgt tggaactgat tcaagaaacg agtttttcgt
    18601 tggaagaaat cgctaatcgt atcggatata aacaaacgac ttctttattc aaattattca
    18661 agaagtattc aacatgtact cccaatgaat ggagaaataa acattaagaa gaaaatcttt
    18721 cactaaagca tatgaaggaa aattgtttta ggtgcgattg ctgtcttatt atatcgttgc
    18781 agcgaatttt tttggattta ttttcagttc taaacttttg gttgtattat caggggatat
    18841 aggtgtaaac atttgtcatt tatctgttat gcgtattcag gaattcgttt ccatcaaaaa
    18901 ttataataac aactattata attaacatgt tgataaaatg aataaacaag actatacttt
    18961 tcatgaaagt aacaaatagt tatgaaaagg agctgtttat tcatgaaaaa aacaagtcgt
    19021 aaagtagtaa tcgtcggtac aggttttgtt ggaacaagta tcgcttatgc aatgatcaac
    19081 caaggaatct ctaatgaatt agtattgatc gatgtgaatc aagaaaaagc agaaggggaa
    19141 gcattggacc ttttagatgg tatggcttgg ggcgatgaaa atgtggctgt atggtcaggt
    19201 ggctatgaag aatgtaaaga tgctgatatc gttgttatca cagctggtat caaccaaaaa
    19261 cctggtcaat ctcgtttaga tttagttaaa acaaatgcgt caatcatgcg ccaaatcgta
    19321 aaagaaatca tgggatcagg attcgacggt attatcgttg ttgcttcaaa cccagtagat
    19381 attttgacat atatcgcttg gaacgaatct ggtctgccaa catcacgtgt tatcggtact
    19441 ggaacaacat tggatacaac tcgtttccgt aaagaaatcg cattgaaatt aaaagttgac
    19501 ccacgcagcg tccacggcta tatcttaggt gaacatggtg attcagaagt tgctgcatgg
    19561 tcacatacaa ctgtcggtgg taaaccagtg tttgaaattg tagaaaaaga ccatcgtatt
    19621 gcccaagatg aattagatgt gattgctgat aaagtccgta atgctgctta tgagatcatt
    19681 gatcgtaaaa aagcaactta ctatggtatt ggtatgagta ctgcacgtat cgtaaaagct
    19741 atcttgaata acgaacaagc tgtattgcct gtatctgctt atttaactgg tgaatacgac
    19801 gaaaaagata tctttacagg tgtgccatca atcgttgacg aaaatggtgt tcgtgaagtt
    19861 gttgaattat caatcaacga agaagaaaaa gcaatgttca gcaaatcaac aagtgcttta
    19921 agagaagtat tgaacactgt attataagaa acaatgaata tgaactaaaa atcctaaaac
    19981 tgcttaacag ctgaaatagc tgttaagcag ttttttattg atatctaata ggtaagcaaa
    20041 taaacagaat ggttaaaatt aatcggtccg aaaatgtatc cgtacatatg aacatatgaa
    20101 atttaatctg ttgattacaa taagcgaatc gttatcaatg tttatctgtg gttaattaaa
    20161 aaatccttcc gcaacggcaa atacaatgat tcatatctaa ttcgttcata tttgattaac
    20221 tgaataaaga aactggtttt tatcattcaa ttaaacgaca aaaaaagtaa gaaaaactgc
    20281 aaaatttttt tataattttg cataagcaga ttttgatgga aaaatgacga ttttt
//
LOCUS       DAZUKC010000047        19634 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000047 DAZUKC010000000
VERSION     DAZUKC010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.048"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(602..675)
                     /locus_tag="ACG80T_001674"
     tRNA            complement(602..675)
                     /locus_tag="ACG80T_001674"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(639..641),aa:Ile,seq:cat)
     gene            complement(865..1890)
                     /locus_tag="ACG80T_001675"
     CDS             complement(865..1890)
                     /locus_tag="ACG80T_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFS4841314.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
     gene            complement(1887..2111)
                     /locus_tag="ACG80T_001676"
     CDS             complement(1887..2111)
                     /locus_tag="ACG80T_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFS4841315.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            complement(2108..2401)
                     /locus_tag="ACG80T_001677"
     CDS             complement(2108..2401)
                     /locus_tag="ACG80T_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841316.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            complement(2439..2576)
                     /locus_tag="ACG80T_001678"
     CDS             complement(2439..2576)
                     /locus_tag="ACG80T_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFS4841317.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            complement(2578..3024)
                     /locus_tag="ACG80T_001679"
     CDS             complement(2578..3024)
                     /locus_tag="ACG80T_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841318.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            complement(3021..3170)
                     /locus_tag="ACG80T_001680"
     CDS             complement(3021..3170)
                     /locus_tag="ACG80T_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841319.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            complement(3187..5313)
                     /locus_tag="ACG80T_001681"
     CDS             complement(3187..5313)
                     /locus_tag="ACG80T_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841320.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPNLMPYITEPWVG
                     PLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTY
                     TLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERDKMVTHTFTGIT
                     KDNVTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIAD
                     PTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYIAAGRVKVGDMK
                     PTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLKLEKGNTRTPNI
                     SEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINS
                     QLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKL
                     YREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSDYTFAITYTI"
     gene            complement(5337..7628)
                     /locus_tag="ACG80T_001682"
     CDS             complement(5337..7628)
                     /locus_tag="ACG80T_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFS4841321.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVDGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFCSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            complement(7638..8375)
                     /locus_tag="ACG80T_001683"
     CDS             complement(7638..8375)
                     /locus_tag="ACG80T_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4841322.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            complement(8426..11857)
                     /locus_tag="ACG80T_001684"
     CDS             complement(8426..11857)
                     /locus_tag="ACG80T_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS4841323.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDFIVAIGGLTAAFGILLPAILAVSYLFGPMML
                     IIGGIIAVIAGVIVAIKNWGAITDWFSGLWKKFTDWLGDTWESIKDGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGTKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSF
                     NPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSVLQEIGDRILS
                     STSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            complement(11874..12056)
                     /locus_tag="ACG80T_001685"
     CDS             complement(11874..12056)
                     /locus_tag="ACG80T_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841324.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            complement(12059..12421)
                     /locus_tag="ACG80T_001686"
     CDS             complement(12059..12421)
                     /locus_tag="ACG80T_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841325.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            complement(12441..13049)
                     /locus_tag="ACG80T_001687"
     CDS             complement(12441..13049)
                     /locus_tag="ACG80T_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4841326.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            complement(13061..13465)
                     /locus_tag="ACG80T_001688"
     CDS             complement(13061..13465)
                     /locus_tag="ACG80T_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841327.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            complement(13458..13859)
                     /locus_tag="ACG80T_001689"
     CDS             complement(13458..13859)
                     /locus_tag="ACG80T_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841328.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            complement(13849..14202)
                     /locus_tag="ACG80T_001690"
     CDS             complement(13849..14202)
                     /locus_tag="ACG80T_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFS4841329.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            complement(14192..14503)
                     /locus_tag="ACG80T_001691"
     CDS             complement(14192..14503)
                     /locus_tag="ACG80T_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841330.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            complement(14500..15375)
                     /locus_tag="ACG80T_001692"
     CDS             complement(14500..15375)
                     /locus_tag="ACG80T_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841331.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            complement(15385..16545)
                     /locus_tag="ACG80T_001693"
     CDS             complement(15385..16545)
                     /locus_tag="ACG80T_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFS4841332.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            complement(16545..17231)
                     /locus_tag="ACG80T_001694"
     CDS             complement(16545..17231)
                     /locus_tag="ACG80T_001694"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFS4841333.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            complement(17194..18372)
                     /locus_tag="ACG80T_001695"
     CDS             complement(17194..18372)
                     /locus_tag="ACG80T_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS4841334.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            complement(18392..>19634)
                     /locus_tag="ACG80T_001696"
     CDS             complement(18392..>19634)
                     /locus_tag="ACG80T_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFS4841335.1"
                     /translation="PDLDKKVFKATKEVIQNIATNSKLRYNTANARTKDGKRPGANRF
                     DEIHENEDYSMINVATSGGGKIRDYREFYDTTNGHVRGGPLDDIIEESKMILSGELGI
                     DKDGAEFSSLFPFICRLDNDNEVDDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAG
                     LRLTFMTKRMNRPMEDTRFAVASYDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVG
                     LLGKYDKDVYFTQHTFIHESALRLQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWF
                     LEMSNKYYIKKIAMDMYRAKILKPALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQR
                     LFFGDDAIMRWYCMNVYEEHISNGNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSA
                     PVTVTNSSVENTGTGFTPLVF"
BASE COUNT         5660 a         4089 c         3008 g         6877 t
ORIGIN      
        1 gactgacgtg ccggacgcac gtagcgatca tcggcgttat gactatataa agtatacttt
       61 cgttgatcca gaaaggtata aagattaaag taggctacct aaaaaggtag cccggaacga
      121 attttatcac catacttgtg aaaggagata ttttttaagt tagtattaag attgtgtaat
      181 atgatgatat ctatatttta tagtatcagt gctataaaat caaaaataag tcactaatta
      241 actaccactc caattgtaag ccttttttct cacttttttt caaaaatatg gtatgctttt
      301 taatggcttc aaatataaaa gagtttaaag cgtaacacac ttatggggaa gtggtttggg
      361 gtgcgcttta aactcttctt tattattatc tcacaattta acccaaatgt ctttctattt
      421 aaaaatcaaa gtaaaacttt tcaaatatac agaagtataa ctatgtgaaa catcctttca
      481 ttaatccata aaaggataca taaaaaagcc actcatttga gtggcaatga agaaaagctt
      541 tagcttgtat aatactcttc aaaaaaattc taacacagaa cgattcaaat ggctacgtta
      601 atgtaccctg taggactcga acctacgacc ggacggttat gagccgtctg ctctgaccaa
      661 ctgagctaag ggtactggtt gttgccacat aaagccataa acaatcaacc agtagaatgt
      721 gtggcaacaa acctgttatc gcatagcttg gagtgtgact atttatgggt gatagtgaag
      781 atatgcgata acatcactat tttatcgaat aatttttata gttgtcaata tagttatgta
      841 ctgctcctca acgaggagct atttttatcg tttaggaata tttaaatacc aacgtttgtc
      901 atggaaatct tgcgcaccgc ctttagtgtt tccctctgga tcattcgttg cccgcatcat
      961 tacatagact ttcttactag gaaaattacg catattgaaa gatacatgat aaccaacatt
     1021 tccagaagta ttataagctt ggtttacatc tggtctataa attccatcag ctcttactcg
     1081 agctaattct ttcccagtat tgtagtccat aatgaaaata tactcgtatt tatagttagc
     1141 aatgtgccat ccagccacat gcaagtttgc gttttcgatt tccccaaact gatcaatgtg
     1201 ggcgtgattt gttccatctg tcagcgtagg attagctgca ccagctcgtg ttggatcaat
     1261 gacaggcttg ttttcagaag ttgttggatt ttcatcggta aatccatgag ctaaatcata
     1321 agcaagcttt tctttgctaa ctcccatttg cgataagtaa ccatatggat ctgtgtggtt
     1381 cccccaaaca taatttgtca cccacaaatg agaaatgatt ccttttgtaa ataaagaagt
     1441 tccttgatca agagtcaatg gaattccata tttttttgca ctatctcttg tatattcaat
     1501 ataagctcga tagttttttt caaacaatgc tttatcatat gtgcgctgta attcaatctg
     1561 tacaggcgca taaggattag cgttaccagc tccccacgaa acatatcctt gctcacccac
     1621 acggtaaaca atcccaccgt caccaataac atctgtagta taagaattgc ttccgttata
     1681 attatttttc atgttggcgg ctacgtttct tgctggtgca tctattccag tttcgtgcaa
     1741 aataattttg ttaggaattg ctaatctgga gtctccttga ttcggcgcta aattatactc
     1801 gtcattaata gtataagcaa acgtattaat gggtaataaa aaaagagccg ttaacaggct
     1861 catcgcagta atagtaattt tctttttcat ttgtttcctc cttcttcgct ttcagccgag
     1921 aacattttgt aggttcgatt tgatacaccc aacacactcc ctaaaaacgc gccaaaacca
     1981 gtaatgatga caacacagat atctgtgtac tgccaattga gcgctttacc aactaacccc
     2041 acgaaagtag ctagtgcggg aataattacc agtgcgaacc attttagtac ttcgaacgtt
     2101 ttattattca ttttcttctc tccctaaata aagttttaat ttgttgcgtg tgttctacca
     2161 atttttctgc atgtgtatct aatctttcat cgtgtttctt tagttcttca tgaatcatca
     2221 atcgatctga tttgctcgat tctaaatctt tagtcagcaa atctaaattg tgacttactt
     2281 ttgaaagagt ctcagtaatc ttcgagaaag atgcagtaat tggttttatt actaataaaa
     2341 tcaaagaaac gatagcggtt attgatcctg ctatcgctcc ccattcccct aaattaatca
     2401 tgtgacaact ccttgaatca aaataaaaag cacatcaatt aagatgcgct ctcttctttg
     2461 ctaatgattt tatctgcttt ttcttcagta atgcacaacg gaacgaaaac cattacttgt
     2521 tcgttagtga aacagcccca atcatacatc attttcacat cgctaaaact aaacatacta
     2581 ctcacctccc tttgaagctg gatttagttg ctctttaatt tctgaaatgt ctttgctatt
     2641 ttgtaacgaa gcaagcatca tttttgaatt gatttgtgct aaactatccg ctttttcttt
     2701 caatgcagta ttttcctgtt taattgctac atcgcttagc atgagtttgg cattgatctg
     2761 ttttaaatcg ccgttctcat tttctaacga ctcatacatt gctttgagat tgtttaaatc
     2821 gttgtgatct agtgcgttcg ctaaaataat ccattgattc aatttaggat caaacatttg
     2881 atcagcgatt gttaacggtt cgccatcagc acgaattcct tcaagcggtg gctgatctgt
     2941 gtaaggaacg gatacaagca tgtcgtccaa tacttttcct gcgtactctc cgccagtacg
     3001 tccatatttc caaatgtttt tcatttattt ccctcccagt aattgaattt cggtttccaa
     3061 tttggaatcg gtggttcgac ttctgtgcat tcttccggta aatgttcttc atcattcaca
     3121 atgatttgct cgaatccgta aggttcaatt ggtctatatg ctgccttcat atcgattcac
     3181 gcttttctaa atcgtgtaag taattgcaaa ggtataatcc gatccataac ttgagtttct
     3241 tcgccattta atggctccat ctgcaccaat agataactga gcactgttca aagtagaacg
     3301 gtctatcgag ccaaccagtt gctcaaaact aattggtggc cgatagcctt ctggaattgt
     3361 tagtatcgtt gaatcatttc caccactgct ttttccgttt aaagccacaa aatatataga
     3421 aactgttttt ccttcacgat aaagctttgc tgatccggta ttcccgtttg taactgttaa
     3481 tgtggtagta gctgtatcat taatgcgttc atcgatctta ttgtctaatt catctatagc
     3541 agtcgcatta gcattcgctt ttgtttgagc atccttagct gtggtgtcta cttcattaat
     3601 tgaagcagtc aactgcgaat taatctccga tactttccca tcggtataat tgtttgcttt
     3661 accagtaatt tcagaaattt taatatctgt ggccaaatta tcttcgacat attctggtgc
     3721 tagatcccaa acataatctt ttggattgtt tgaatcacgc ataccagtac cacgatattt
     3781 atactcacta atattcgggg ttcgagtatt gcctttttct agtttgagcc actcaatttg
     3841 aacagctcct tttgtagctg acgggtattg gtatatttcc agataattag ttaccccact
     3901 atctatatgt tgcttagtta cagtaaaagt taactcccat gtattagcta acccctcagt
     3961 aggcttcatg tctcctactt taacgcggcc agcagcaata tacacggcaa acgattgaac
     4021 tgttagttta gttgctttca ttgatatggt atatgtttgg tcagcgatat agttttctgt
     4081 attgcgtcta ccataaacca tatattcact agttttgtgt ggaaagacaa ctgtagggtc
     4141 agcaatattc tcgcctaaag gaactttacc taaccagtaa gggccatcta gtaagttagg
     4201 gtagtgtgct tttaacttaa cgtcatagct gacactaacc gttcctttat attcactatt
     4261 aagtgagaat atttcaacat aacagtttgt tatgctagtt acattatctt ttgttatacc
     4321 agtaaatgta tgagtgacca ttttatcgcg ttctacattt gctggtatta atgcattaat
     4381 gggcagttct ataccacctt gttcatttaa atatctaagc ctaaccccta tagtattggt
     4441 ctctccagtc caatctgaac cgacactcat agtaacgctt aatgtgtatg tcgtagatgg
     4501 tcgtaaagtg gttataagat acctattatt agcttccgct gtacacggaa tacctaagcc
     4561 aagcgataat atgtcgccgg aattagctgt tctagtttta gtatgagtta taaccctttt
     4621 aacactatct ttaactgtat gtccattacc taacaaaggt ccaacccaag gttctgtaat
     4681 atatggcatt aaattcgggt tctccgaata atcatagtcc ccgaagtcga tgctgttact
     4741 gtacatcact tgtaagttac ctaacttaga aatttcttct ttcagagcat ctaacttgtc
     4801 ttgtagcgtt ttagcttgac cagttaaatc agtaatctgt tgatttaagc tatccactct
     4861 acctttgatt tcagccataa aagcatcaaa agtttcgttg tactttcgaa tcaactcttc
     4921 taattgcgaa acatattcat cggcttggcc ttgcgaaatg tcagacactc ctagtgagaa
     4981 aaaaatgata tcttgcgttg ttaaaatttg attgtctttt ctatattcta cgtagcagtg
     5041 tttataatat cctgcttcac tcataaatgt gccatcaaga gaaaacgtga cttcttcact
     5101 agttacacta gttgcaacat tatctacgta acggttagat ggtgttgttc cttttaaagt
     5161 aaatgttccg ccactcgtat ccatctgcaa gccatttaga aacggtttaa ccgtcaccgt
     5221 aatcccttta tcaccttgac gagccataat agctttggtg tagtttaatt ctttgctgaa
     5281 atctaaagcc aaattatata aactgctagc catttatata cctccttgtc ttcgttttaa
     5341 aaacgttttt ggtcaagcac tgtgctatca tatgctgtat cctcttttaa tctaatatct
     5401 tcatacccta gacggtgtgc cactaaattc catctaacta atacgtttgg cttactagtt
     5461 tcaatgatga aatggtcaat atcttcatga gtaacagcac acaaaactag ttctgtaggt
     5521 gtcacatgtg tcatataccg acttagattt actgtttcag caaacatggg gtcaatatca
     5581 acacgaactt taccattgtc acctgtaacg gcttccccat aatcagcgaa ataatattct
     5641 ggagtttcat aagcgttcaa tagtcgttgt ccataatgtt ctgttggtac agttgagttt
     5701 ttagtacctc taacagtaaa atctttatat acttgtaccg ttgattgttc aaacctagca
     5761 agtttcccat cttcccatga acaaaaaaaa caacctggta acgttagcat accatcacta
     5821 gtaaatttca tagtcctacc agctacctta aattcccatg agttacccgc actaccatta
     5881 atgcttaaag aactaccgtc gccagaagtt acataactag cattgctata cctgaaattg
     5941 ggcgcaccaa aagatagaaa cggtctgtta ttaccattat cccacgtact aaaaaccaag
     6001 ttaccctgtg gatttctaat catgaaacca ccaccagttt tcatggtgta tgatacaata
     6061 ccggcatcag cacttacata atcacgtgct tctagctcca taatatcttt gttaactttt
     6121 tttgagtacc aagtcatttt gccattagca atacttgttc tataatcagc accatcacta
     6181 attaatgtag tacctctaat agtaattcct actatttcac cagccgtaat aaacgaggca
     6241 ttgaatccgc catctaacgt ccatgccgtt tcatatgttc cattaatgcc agttttagaa
     6301 aaaccaatac cagcattgtt gatttgtaaa acattccttg cggtattctt atctggtgtg
     6361 tccataatca aaatacgact aggcgcttct ttaggatcta ataaaacata accaccattt
     6421 tgaccagtaa tcatatcagt ttgatgatct acaatatcat tgagtaaatc actgatttcg
     6481 ccaccgtttt tcaattgatc aatggcatca ttaatcaaat tgctgacatt attctctgtg
     6541 ttttctaaga agtttgtttt gacgtttcct acaactaatt tatcgtatga attggttaga
     6601 acattaaacg tatattccac aattctcgct gacatattca cttttaactg tggatgatac
     6661 acatctactc cgtcacccat cgaaactttt tctagatcaa caaatttttc atagcctctt
     6721 tgatgcctca atggtactaa ttcaatcgaa ccactcactt gtggtttttg tttatctatg
     6781 tttgttttca accagtcttt agcagcttcc cttaatgtgg ctacatcagt cgctttgtct
     6841 ttaaaatcaa caaaagaaac atatccagca ggataatcat ccacgtaatc cgtgaaaata
     6901 acttcttctg gtagagtgat ctcgtcttct ccttctgaag agctgctaat gaatggataa
     6961 actccaacta aaacactttg agcatcaatc tctaagtcaa gaccagttaa gtttttagta
     7021 taaatcgctt tgattttatg atccgtacct agactttttt catgacgtaa tgtgttatta
     7081 tcttttagaa actcaccatg aaatcgatct agaatagatc cctcttttcc accaaagaat
     7141 tctaaaaaat tcgccttttc tatcttcaca ttagcaagcg tatctactaa tgacgagaaa
     7201 gaaaactgcg aagggatagc tggtttcgct aaaacttttg cgttttgcca tgcctgagta
     7261 gcagtgatct tttctgttcc gctgtcatat ttattcaaca ccgattttct tatatcattg
     7321 aaaataggtt cagcttttac ttctatcgta ttgcctatta cagaagtttt tgcataataa
     7381 atccgtagac gctgttttgc tcgattttca tctacataac actgaataat acgtccgtct
     7441 acaatcaaat ctgcattagt tccgtttatt gaataagtac cctgaaatat ctcggctccg
     7501 tttagtttat tgctaacagt agctgttaac cagtctgaca aagcgcctaa accttgcgta
     7561 tcatataaat gttcagctaa attattcgcg tcatttttat cgtaaatagt tattaaatta
     7621 tcgatcatct atttcaccta ccttaacccg ttacgataaa tttgtatttt gctcaaacca
     7681 gtgcaattaa aatgattgat atccacttgc aatgtcggat attgcatggt cttcattttg
     7741 ttggaccgat ctaaaatatc tccgtccgat tgctcttcgt agcaaagcat caaatcacta
     7801 tcaatgacta catcagttcc tgctactaag ccttcgaaac taaacacata atcatttaag
     7861 atgaactggc atgaagtagc tgaaggagtg atgataatct ttggaaaact ttcttctaaa
     7921 ctattattca gcaagttaaa tgactgtggt ttatctacgg ttataggtac atcttcttga
     7981 actcttgcga atggtttcgc agtaatattt acatcgaact ctccccattc aacaatatcg
     8041 ttttctgcat ccccaatatc gatagtctgg ataacgtaat agacgttggg atcgtcagag
     8101 aattctaatt tctttgcata gtttaaccaa tgacgcatga tataaaacga ttgcttgaac
     8161 gcttgatggt cttccacatc ctctaaatag ttatagtgca atgtaaacga catatcttca
     8221 aacgagtaat cttgtactaa gccacctaac ctacctaaaa cagaagtttc aactctctgt
     8281 ctttttggag aaggtatggt tggtctttca gctaaagcca atttatgcaa ataatcagga
     8341 aatccatcga ttatagaatg tatacaatca gtcatttttt cacatccttt ttaatactaa
     8401 aaaaacagga gaaatactct cctgtttaac gccatgccga agcattatca ttttgaattt
     8461 ttgtaatgct atcaatgatt tgttgagttg tttgcttcat agtaacctca tctgcgttac
     8521 catcaattgt gaaattgaat tcgtagttat tcacaggttg aatcgtttgt gccctagatg
     8581 aaactgatgt gctactcaag atacgatcac caatttcttg caaaacagat cttttcaaag
     8641 gtaaaactgc ttcaggtcct gcttcaccga caccgttcat tccacctagt aaagttggtt
     8701 tagtaaagat acctccttta gcatgccatt ttacacgcaa atgggggatt tgacctttta
     8761 gcgggttaaa gctgccttcc atgataaatt ccggtaacgg aatatgtggt atagaaatat
     8821 tcaaattatc aaaaatgcca ctgattttgt ctctaatcca atcaattgga gcgctaacag
     8881 tctttttggt gccttcccat atgttagcaa ttgtactttt aacattattg aatatgtcgg
     8941 aaacaatacc tgttagattg gaccaaccgc ttgaaattgc attttttcca tcgtttactt
     9001 tagagctaat agtgcttgta attccattcc aaagattcaa agcagtattt ttgataccgt
     9061 tccaaattcc gctaatccac gaagatatac tattccaaac actttgaatg gcacttttag
     9121 ctgcgtttat agcattgctt atactactag tcacactatt ccagatattt gatgctgtag
     9181 agctgattga attccaaatt ccacctaacc aactagatac agttgaccaa atattttgaa
     9241 ttactgtagc agctgcttgt accaagctag tgattgtatt cttgatactg ttccaaatac
     9301 tagaagctgt tgcactaatt gaattccaaa tatttgaagc cgtagtacta atagctgtcc
     9361 atataccatt ccaccatgcc actactggat caaatatagt atggaatgta gttacaatcc
     9421 cattccaagc gatacttatc cattgtgtca tagtatccca agtattttta aggaaatcag
     9481 aaataggtgt ccaaacagct tgccaagctg cgcccaataa ctgtccagct acatcaaaaa
     9541 tacccacgat aatattaata ccagcttgaa tcaatgacgt tattaatgtc catggtattt
     9601 gaacaattcc tacaatgtct gcccaaataa tcgaccatac ttctttgact ccgttccaaa
     9661 tatttgaaac ccaatcaacg aatgtttgcc aagtttcttg gactccttgc cagatgttgg
     9721 aagctccttc aactaatccg ctccatagct ctccaaacca atcagaaact ccttgccaaa
     9781 tatcttgaac ccaatctaca aatccagacc aggtttcttt aaccccatcc caaactgatg
     9841 aggctccgtc ttttatactt tcccaagtat cacccaacca atcagtaaat tttttccata
     9901 aaccactaaa ccagtcagta attgcacccc agttcttaat tgccacaata acgcctgcta
     9961 taacagctat aattcctcca attataagca tcatcggacc aaataaatat gacacggcta
    10021 gtattgccgg taacagtatg ccaaatgctg ctgtaagacc acctatagca acaatgaaat
    10081 cctgtactgg ttgtggaaga ttattaaacg catcagccat ctttcctaga aaatcaatta
    10141 ctggttcgag tgcatctatg attgtgttgc ctataggagc taatgaatcc tttaattcag
    10201 ctattttccc gttcaactct tgcaacggag tagtagaatc ttcattcatt ttttgtgcag
    10261 atccactaac atcatcaaat gtatggttaa catcagttaa agattggaca actttcatcg
    10321 cattatcttc gccaagcgca gaccaaattg tcgaagcttt atttaattgg tcgtattgac
    10381 catccatatt gctaaaatct tgaatcatgg aattaataac gtctttttgt gttcctccgc
    10441 catttttcca ctcttcaaaa gcttttctag tactttcact aaacatatcc atgttttgct
    10501 caaatcgacc atctgttaac gatattccca tttccttaac taagtcattg actttatcaa
    10561 ggttataagc acccgcatct aaaccatttt gaagcattcc gaacgtttca tcagctgaat
    10621 atcccatttg actccataat tggctatatt ctgccatatt gtcgcctaat tcgtgcgttt
    10681 tatctaaacc gttttgagta cccgaaacca ttaaatccat tgcatcttga gcactcaagc
    10741 cgaaattgac cattaagcca tttacaccac gtaacgtttc atccatatca gcgcccatgg
    10801 tgttttctag gaccatagct tgttccgtga tattttgtaa atcttgatta tttaaatctc
    10861 ctaaattacg ctttaccaaa atcaatgcat ctgtggactg atctaacgat tctccaaaac
    10921 ctttataata aatgtctctg gctacattcg ttaattcttc agcctcttgt ttagacaaac
    10981 caaaattagc ttgtatctta ctctgggaac tacctacact gttagcagag tccactgctt
    11041 gtttccctaa ttctgtaagc ttatcgccaa tgtcgcttaa aacgtcagaa gcttccatta
    11101 aattattcat atctattttg cttccgatat cgtccaagtt agttgtatct acatttttag
    11161 cagcttgtcc taactcttca aattcacgtt cagcatcatt aagcttcgct tccatctgca
    11221 ttgcttctgt ggatgtagcg ccaaattcag actgtgtagc ttctaactgt cgtcttagga
    11281 tatctatcgt tttctccgca ttttcagatt gttgagaaac atattcttgg gctttcgcta
    11341 atttctcggc ttcagaagct gattgaccag cagttgcttg ccattttttg tattcggatt
    11401 caatcagaga agcactagct tgaacatttt tttgttcgct atccaactgt tgcattgtag
    11461 actcgtacgt ctgtatttcg ccttttgctt gagctagtgc attactcgtt ttatcaattt
    11521 cgtttgacaa acgttgttgc gctgtttgtt gattaatcag ttctctttca agtttctgaa
    11581 cttcggtgga attttctcca taatattttt tcgcattggc taaacgttgg ctagttactt
    11641 caactttttg actttgtaat tcatactgct tttctaaaga agataattta cttcctaact
    11701 tgtctgattc agaaccagtc tgttgtaatt gagcttgttc tagttttaat tctgctctat
    11761 ttttagttaa ttcagcactg atttctttta acgtagattt caatccgtca tcgttagcta
    11821 tgaaagttac ttctgcttct gttctctttt tagccatttt ttacctcctt tctttagttt
    11881 ttctgggatt ggtttattgc atagtttttc catccttcat aagcactctt gttgtaagcc
    11941 atttgcaaaa tgtcatctaa acagatatcg cttaaaacca aatctgaagg catagaaaaa
    12001 acgtcggtca acatcgaata gacatcgacc cacgtttcaa ctaagagctt tggaattttt
    12061 acttttgaag ctttttttcc ttatttgctt tttcaaattc tttttgataa gcatcgcgtg
    12121 cttgtttgaa catcatcaat tgataaatat agctggcagt agccatatca aaatcccatt
    12181 tatcgataaa ttcatcgaat gaaatataat cagtcatgtt cgcttggcgg taagcaatat
    12241 acacagcctt tgcaccttga ataacagaaa tatccatgga tccttttccc acagtcattt
    12301 ttgcaaactc gtctgtgtta aaatctctat tgatcatcaa taatttcttg atattcagtt
    12361 taggttctaa attcaaaatt gttccatcgt ttagttcaat ttttgagtaa tcttcgttca
    12421 tttcgctacc tccgtttttt ttactgtgat tgagtggccg tagttgtcac aactgaagtt
    12481 tttttaatca catcagcaga tagattcgtc atccattgat ctgttaagtc ttcttcaagt
    12541 tctgcaacaa ttgcttcatg ataaaattta ccaaattcat cttgcataac ttttgtttct
    12601 agttctaacg cagctacttc atccgcacca ttttcaatag agaatgttaa tcctgtattc
    12661 gaagtgcatg ctaacatacc aactaacttt ctattttctt cgaagtcatc cacgatctct
    12721 gcagcaagtg agaaatcttc gcctacggaa tcaggaccgt aagagtaaat gcctggttta
    12781 atacgtccat cttgtttcaa cccattgaaa cgtcgataaa cttccatcgg tacatgtgca
    12841 gtaattgtta ccgtcatatt gattggttta gattttgatt ttacttctgt cgctccacat
    12901 tttttaacca ccgtttgcat ttctgtttcg ccatctaatt gtccattaca attcgttgcg
    12961 attgcatttc ctgcgttctt aaaattaaaa gcaattcgtt tgatacttac gttatcgaac
    13021 gttgttacta cagtttttgt tttagccatt gttgttcccc ctatttattt aatttatcga
    13081 attgacgaat cagaagttct gtaattggat caagtgcaag acctaatcct cttctcataa
    13141 attcgtccgg ctgatttctt ttagaagtac ctatccccaa atcaggatat tttaaatact
    13201 caaattttct tgtaggtcta atgatgaaac ccaaattaat gtattgagtc ttaagtggac
    13261 gactattttt tgcgtgttgg tgccctcttc ttaaatctgc ttcagaaaca ggaatttttt
    13321 ctgtaatcct atccactgca atagccgaac cttttgattt caatgcttcg ttaatcagtc
    13381 gttcgctctc gcttgaatag cgttccatcc gcacaagaag ttcatcatgt ccatttattt
    13441 ttagctccca actattttta gccatgacaa tcactcttca ataatcgtct aaacgtaaat
    13501 accaattgat cgatatagcg atcttggttc tctagtttta aatgattggg atccattctc
    13561 tgaaaacgaa tcgaacgatt ttgaatcaat gaaataatat ctagtgagtc tcctgttaaa
    13621 tcttctctat tttctgaata gaaagttaga tatagatttt gacccacgct atattttggc
    13681 tcagtgatca tttctatttc tcctgtttcg agaatgaagt aattaaaatc atcaggtagc
    13741 tcatcctcgc ctacggagtc ttgaaagagt ttgaggccaa aatgctcttc taaggaagtt
    13801 ttgatagcag aaatttgctt atttaaacgt tctttttctt tagaattatc aatcaccata
    13861 ttcacccaca ctttcaagat aaaaatagat ataaaaatta tcgtaatcgg catagataac
    13921 gttgtaacgc atactatcga ttacgataaa atattgatct ttattaaatt tcttggcgat
    13981 tggatgaaat ggagtcttta ctttcttagt taatttcgat cccatcgcat ccatagctgt
    14041 tatatcacta tctctcatgg aaagatttct aaattttaaa gaagtgattt ctgtatcttc
    14101 tacaccaatc ttttttccta gttcatttct tttggtagtt tgcgtcaaaa tctttaacca
    14161 accatcgttg aatgtttctt cgagtctacg attattcgcc attcacatca cctgcaatat
    14221 attcttgtag cgcataatgt tgaatgaaac ctaataactc actagcgaaa ttttgttcaa
    14281 actcatctaa agcacgattc cagtcgtatc tacatctttc gattagcaat ccgtattcta
    14341 agctttcagg agaaaaagaa agtgttgtac tcactttact ttgaagataa acagcatttt
    14401 tagctatcat ctttttaatt gactcatctt cttcgttcca ggtaacgtaa atattatcct
    14461 tcacagctat tagcaattct tcagtcactt gttcaggcgt catctaacca ccgccttaat
    14521 tgctttaaca tatgcgtaag agcatttttt cttgtttaca aatgataaat cttcatcaaa
    14581 aggcgtagaa gtcacgtatc tccctttgaa aaataaatct tcatcgtttg ttgttactcc
    14641 agcattgtgt aagattttta cttctttaac tttttctatt ggatcagtag caaaacaaaa
    14701 gtctaattcc tcgtgaactt taggaccaat attgaaatac atcatgttcc aaagctgtgc
    14761 ccacatctcg gctgtccaga tttgtatatt tgttttttgc cctctaaggt agcgatatag
    14821 ccgattagaa tccagataaa cctttttcca ataattcgct ttaggacggt taataaccca
    14881 ctgtgcgcct cctgaattag tgtttatagt ttccaaagat tctactgtaa catttacaat
    14941 gtttgccata tcttttagaa tattttctcc gttttcacag cttctaatat aatcaagact
    15001 tagataacta cagcagtcgc tacaatacca aacatcatct ttagaaggca atttgcgcaa
    15061 attaattctt ttattgaaaa tgacatccga atcgatatag aaatatcggt cgtcctcacg
    15121 cgaatgatct tcttctaaat atttccacca taaatatggt ttaatcgaag gaatatactc
    15181 tttgtcgtcc cgcagatcat cgtacacatg aacttcaaca ccatattcct tctcaaaaaa
    15241 aataggaatc tgatcatcgt gtctgctgaa aagcaatacg atatctttga ttcctagttt
    15301 cttcagatta gttaaacaaa cttcaagctc ccatttaaac cgattgattg ccggctgaca
    15361 aagaatatac ttcattctga tcacctacgc ttgtgttgta gttgttgtgg ttgttggttt
    15421 tgtagttgta gtagtagttc ccaaagcgct aatatctaat acaatgaaac tatcgttacg
    15481 tttaggttga ccgtttgcat attgtttagc tagataaatg cgttcgtctt caacaaaatg
    15541 gtattcatct gaagcttcaa tttttagtgt agatcctaca cccatgaagt aatctgaggc
    15601 taccccaata actgcttttc cttctggcac agccgttgac tgcaaatctg aaactggtac
    15661 tggcaatact tgtacgtatt ctccattagc agttagtaca gtcttagctg ggaatacttt
    15721 agaccagtaa tcagttggat tcacaattag gaccacatca gaaggattca cattacgata
    15781 aatcggatca ttcacacctt cgatattgaa ttttgatagt cgcgccatca aaccgcccat
    15841 agttacagca tctaaagctg taataggttc tgcttttttt tcagcatatt ctccgctagt
    15901 ttgtttgctc atgtcacgca tcattccgac tggcatatct ttaccagtac catcaacaat
    15961 tgcttgttct aatgcaattc tcaatgattc tactaaaaca gtacggacat aacgatctaa
    16021 ccatactgga cctaaatcaa gcattgcctt acatacagga atataacctg atagcttgaa
    16081 ctgcttcatg ttaattacat caaagccatt atctaaaact tttttaacag cttcgcaaag
    16141 tttaccccac catgctggat tgactccacg tgacacaatc cattctgtta caccagttgt
    16201 gttaacaaaa gtaatttttt gcaatagtgg atgagattgt tctaaatctt caaatacacg
    16261 ttcaaataca gtagctggca ctaattcttc gacccctgca aaaccttcgt ttttcactac
    16321 ttcgttatag aattttgttt cttgtgtagt taatacacgc tgaccacggt tcattaatac
    16381 taattgatct tgattttttg ctgttgcttc ttctaaaatt ttatcctgaa tttccttaga
    16441 taagcttacc atagctgcgc taaaagattc ttcgttacca tctttaaaag ctttcatcaa
    16501 ttggtcgctt gcagctgtta caccttttaa atttttaact gtcattattt tacatctcct
    16561 tgtccaaatg ttttatttaa tgctgctgta aatgcagcaa ttttttctgc tcttttttct
    16621 ttaacgtcat tcaaaatttc ttcaacgctt tgttcttttt tagcttcagt acctgagcta
    16681 ttttctgcat cgataatttc atcgaccaat ccataactca aagctgtttc tgcatccata
    16741 aacgattctt tttcaagaag ttcttgcaat gcttcatctg tgccattgaa tcgtgtttta
    16801 tatgaagcct ttaccgattt atcaattgat tccagttggt cagcaatcgt acggaagtca
    16861 tcgacatttc cttctccgta tgtggaagcg cggtgaatca tcaattgtgc attgttgtag
    16921 atttttatag tatcgccagc cattgcgata attgaagcag cactagcggc taagccgtta
    16981 atcacaacgt taacttttgc tttatttgac ttaagtaagt tcccaatagc aatcccttga
    17041 aatacgtctc caccgtttga attaattact acttcaattt cttcttgatc acctagacta
    17101 tccaaaatat ttttgattcc cttgtcagta ttcccttcaa agaaccaact agaaccaata
    17161 aatccctgaa taaaaatttg cggtactgcg ccttcattct ttactgctag aaatgttttc
    17221 attgtcgtca ttcgcctcac ctcctttcga tacttgttga ttgtttttag ttataaatat
    17281 ttcatctgcc atcgccttat cagagcgatc atttccaacg cgttctcttc cttcgttgat
    17341 tgtaaatact ccatttctaa tgcctacatc aatagcgtca accaaatctt tgaagctagt
    17401 aatcttgatc atagttgtat ccacacgtac aaaattccct gacaagtatt cttctacttc
    17461 atagagacta gcgttaaacg catcctgaat aagttcagca atcggtatga tttcgaacat
    17521 taaaaaagcg tccacttgat ccgataaccc actcatgtct ccctttagta ggtttttcgg
    17581 aacgtgaaac gctgctgctg tcatctcaaa gatgtcgtct attaagtttt ttatatctct
    17641 tgaattgctt tggaagtttc cgctgaaatc ttctaatgtg tactcatttt gtaattgaaa
    17701 taccgcacct gcattatcag cttccataaa agccttaaat tgtgatgtca tcattttatt
    17761 gatttgatct tgtgttgtat tgtcttgcgg tcggaataaa ttccctttca gtacgtatct
    17821 acgagcgtta gagcgcttgt aaacattcat ggcactagaa atgagtttcc catacgcttg
    17881 ataatacgca tcgactagtt gcctaatttg ttgatctgcg tattttatat agataacatc
    17941 actttctaga aattctctat caaggactat gttgttaatt tgcacttgag aaaacacatc
    18001 atctttcaat gcatattctg tgacatccca actatccgca ataaatattt cgctagaatt
    18061 attagacgga gaaacgatca atacttcatt gtagaatatt aatctcctga tcagtttttt
    18121 tctaaattct gttgcattat ttttcttatt aggagctaca ttcagcctat agtaaagatc
    18181 attcttttta ttttttccat cttcatatga cttgaattcc gctttactca tcgcatttgc
    18241 aatcaaatca atacaagttt caatcgcaaa ttttcgatac acaaaatcaa cttgcaattt
    18301 acaaaagtat tcttctaaag gaaccgttgc tttttttgtg aagtatccta ccgccttttg
    18361 aaaaatcccc actttctcac ctcctttcaa gttagaatac tagaggagta aatccagttc
    18421 ctgtattttc tactgagcta tttgtgactg ttacaggagc agaatcataa atatcatcta
    18481 aaaaattcaa accatgaagg aatgaaaaaa agccatccgt ttttctagtt tcaggttcta
    18541 ttttttcata gcgtatattt ccattagaaa tatgctcttc atatacattc atgcaatacc
    18601 aacgcataat cgcatcgtca ccaaaaaata aacgttgatt aataaaaagg tcatcaacca
    18661 gatcttttaa cataccatgt gtaacagatc cgcttcgaac aatttccaca gtaaaacctg
    18721 cttcttctaa agcgggcttc aatatttttg cacggtacat atccatagcg atttttttaa
    18781 tataatattt attactcatt tcaagaaacc aacctacaat ataatcagct tctatatttt
    18841 ttccatgaac gatctgtgat tttccttgat ctatagaaat atctataacc tctcgtttga
    18901 tgttttgtaa tcgaagggct gattcgtgga taaaagtatg ttgtgtaaaa taaacatctt
    18961 tatcgtattt tcctagcaac ccaacgctgg caaaatctcg tctatcagca aaatcgactg
    19021 ttcctatcac ttcatccatt ttttcaggaa attctttttc tttcgtatgc agaacatcat
    19081 catatgaagc aacagcaaat cgtgtatctt ccataggtct gttcattcgt ttggtcatga
    19141 acgtaagtct taaaccagca ttacgttgca tttgagagta ttcttgaaac attttccgtt
    19201 ttaaatctgc attgtaatta atagttggac aagctttttc ccacatgtcg ggatcatcaa
    19261 cttcattatc gttatccaag cgacaaataa atggaaacaa actagaaaat tctgctccat
    19321 ccttgtcaat tccaagttct ccagaaagaa tcatttttga ttcttctata atgtcatcaa
    19381 gcggaccacc acgaacatga ccattagttg tatcataaaa ttctctataa tctcgaattt
    19441 taccaccacc agaagtagcc acatttatca ttgaataatc ttcattttcg tgaatttcat
    19501 caaagcggtt tgcacctggt cgcttcccat cttttgttct agcatttgcc gtgttataac
    19561 gaagtttgct gtttgtagcg atattttgaa taacttcctt cgtagcttta aatacttttt
    19621 tatctaaatc agga
//
LOCUS       DAZUKC010000048        18552 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000048 DAZUKC010000000
VERSION     DAZUKC010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.049"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            147..794
                     /locus_tag="ACG80T_001697"
     CDS             147..794
                     /locus_tag="ACG80T_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFS4841336.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    923..1139
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1187..3832
                     /locus_tag="ACG80T_001698"
     CDS             1187..3832
                     /locus_tag="ACG80T_001698"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFS4841337.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4142..5455
                     /locus_tag="ACG80T_001699"
     CDS             4142..5455
                     /locus_tag="ACG80T_001699"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFS4841338.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5445..6098
                     /locus_tag="ACG80T_001700"
     CDS             5445..6098
                     /locus_tag="ACG80T_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4841339.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6153..6845
                     /gene="radC"
                     /locus_tag="ACG80T_001701"
     CDS             6153..6845
                     /gene="radC"
                     /locus_tag="ACG80T_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFS4841340.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6995..7195
                     /locus_tag="ACG80T_001702"
     CDS             6995..7195
                     /locus_tag="ACG80T_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4841341.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7449..9128)
                     /locus_tag="ACG80T_001703"
     CDS             complement(7449..9128)
                     /locus_tag="ACG80T_001703"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFS4841342.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9130..9342)
                     /locus_tag="ACG80T_001704"
     CDS             complement(9130..9342)
                     /locus_tag="ACG80T_001704"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFS4841343.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9735..11465
                     /gene="efrA"
                     /locus_tag="ACG80T_001705"
     CDS             9735..11465
                     /gene="efrA"
                     /locus_tag="ACG80T_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFS4841344.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11462..13222
                     /gene="efrB"
                     /locus_tag="ACG80T_001706"
     CDS             11462..13222
                     /gene="efrB"
                     /locus_tag="ACG80T_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFS4841345.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13317..13514
                     /locus_tag="ACG80T_001707"
     CDS             13317..13514
                     /locus_tag="ACG80T_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841346.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13667..14152
                     /locus_tag="ACG80T_001708"
     CDS             13667..14152
                     /locus_tag="ACG80T_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFS4841347.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14256..14849)
                     /gene="clpP"
                     /locus_tag="ACG80T_001709"
     CDS             complement(14256..14849)
                     /gene="clpP"
                     /locus_tag="ACG80T_001709"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFS4841348.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            15029..15550
                     /locus_tag="ACG80T_001710"
     CDS             15029..15550
                     /locus_tag="ACG80T_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841349.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDV"
     gene            15647..16384
                     /locus_tag="ACG80T_001711"
     CDS             15647..16384
                     /locus_tag="ACG80T_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFS4841350.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16388..17305
                     /gene="miaA"
                     /locus_tag="ACG80T_001712"
     CDS             16388..17305
                     /gene="miaA"
                     /locus_tag="ACG80T_001712"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFS4841351.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17307..18536
                     /gene="hflX"
                     /locus_tag="ACG80T_001713"
     CDS             17307..18536
                     /gene="hflX"
                     /locus_tag="ACG80T_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFS4841352.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEIAVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5994 a         3209 c         4033 g         5316 t
ORIGIN      
        1 gtttggttga gcttattacc gcagatgcaa gcttttgaac accgttttat ttgtctcaca
       61 cccttttcct tcctacttgt aaccttgctc ccgtgttaga aacatgttac actaaatatg
      121 tgaaattatt aacaaggagc gtcgttatgc gagaaaaaac agatacactg ccaaaagcaa
      181 ctgcgaaaag attaccttta tatttacgct acttgaagat gctggatgac tcaggtataa
      241 gtagaatcaa atcaaatgag ttcagcgaaa tcacccaaat tccttctgca acgatccgca
      301 gagacttctc gcaattaggc gagctaggca gaagcggata tggctatgat gtcccttttc
      361 ttatcgatgt attcaataat atccttaata ctaaagaaga aaaacgaatc gcattagtag
      421 gatatggaaa tcttggcaaa gcattgaaac acaacaattt tagaagaaat gaaaatctaa
      481 atatcgtgtg cgtctttgat aatgatccag cactgatcaa tcgagtaatt gatggagaaa
      541 tgatctatcc gattgatcgt ttcgcggaga tagccaaagc aaaaaatgtg acagtagcaa
      601 tctctacggt accaagtaaa tattcacaat ctgctattga tgaaattgtc aaaggaaatg
      661 taaccgctat acttaatttt gcgcctgaca gagtaactgt cccagcttat gtcaatgttc
      721 aatacattga tttgacaacc gagttgcaga cgcttattta ctttgatgag aattactcag
      781 aagttttttc ttaggaaaat ttgagtaggc ttattgatct tgcgagaact cgtcgtaatt
      841 ctttcaaaaa ttttttagta ctaaatgaag tggttttttc acgaccactt gttgactaaa
      901 aaaaaaaacc attgtagaat gaagcataac gctgaacaag aggagtaact ggcacattca
      961 ttcttagaga gagaactatc tgctgaaagg ttctcaatga caaccagtga aggtagcttg
     1021 ggagtttctg tcttgaatgg agtagagata gacgagtcgt gatcgttaac tcacttgaga
     1081 ggcaataatg attgattatt gcaatttagg tggtaccgcg taaatcatac gtcctagaat
     1141 acgaaagtat tttaggacgt tttttagttt aaaggaggaa aacagcatga cggaagaaaa
     1201 taacctatca acaaaatata atccgcaaga agttgaagct ggacgttatc agcattggtt
     1261 agaacaagat ttgtttaaac cgagcggaga taaaaaagca aaaccttatt caatcgtaat
     1321 cccgccccca aatgttactg gaaaacttca tttagggcat gcttgggata cgaccttgca
     1381 agatatgctg attcgtcaaa agagaatgca aggatatgat actttatggc tgcctggtat
     1441 ggaccatgca ggaatcgcga cacaagcaaa agtagaagcg aagcttagag agcaagggat
     1501 ttctcgttat gatctaggtc gtgaaaagtt catcgatcaa gtgtgggaat ggaaagaaga
     1561 gtatgcgagc cacatccgcg aacagtgggc taaaatgggc ttatctttag attacagtag
     1621 ggaacgtttt acattagatg atggattatc tgaagcagta cgtaaagttt ttgttacctt
     1681 atacgaaaaa ggtttgattt atcgtggtga atatatcatc aactgggatc ctcaagcacg
     1741 aacagctcta tctgatatcg aagttattca taaagatatc gaaggcgcct tttatcatat
     1801 gagctatgaa ctagcagatg gatcaggcgt agtagaaatt gcgacaaccc gtccagaaac
     1861 gatgttggga gatacggcga ttgctgttca tccagaagac gagcgttatc aagcgctcat
     1921 cggcaaaaaa gtgattctac ctttagtcaa taaacaaatc ccaatcattg cagatgaata
     1981 tgtagatatg gaattcggga caggtgtagt aaaaatcact ccagcacatg atccaaacga
     2041 ctttgaggta ggtaatcgtc atgatcttcc acgcgtaaat gttatggatg atgatggtac
     2101 gatgaatgaa ctagcaggaa aatatgctgg aatggaacgt tttgctgctc gtaaagcaat
     2161 cgtgcaagat ctgaaagaaa tcggtcgatt gattaaaatc gatacgatga tccacagtgt
     2221 gggtcattcc gagcggacag gtgtcgttgt tgaacctcgg ctgtctactc aatggtttgt
     2281 aaaaatggcg ccgttagcga aaaaagccat tgataaccaa gaaacagatc aagcagtgga
     2341 attcttccct ccaagattca atcaaacgtt tttacgttgg atggaaaatg tccatgattg
     2401 ggtaatctca cgacaattat ggtggggaca tcaaattcct gcatggtatc acaaagaaac
     2461 aggtgaaatg tatgtaggga tggaagcacc agcggatagt gaaaactggg tacaagatcc
     2521 tgatgtttta gacacttggt ttagttcagc tttgtggcct ttctcaacaa tgggctggcc
     2581 agatacggaa agcgaagatt ataagcgata cttccctact agcactttag taaccggtta
     2641 tgacatcatt ttcttctggg taagcagaat gattttccaa agcttggaat ttactggcga
     2701 acgtccattt gagaatgtct tgattcatgg attgattcgt gacgaacaag ggcggaagat
     2761 gagtaaatca ttaggtaatg gaatcgatcc gatggaagta atcgaaaagt atggagcaga
     2821 tgctctccgc tggttcttat cgaatggttc tgcaccagga caagatgtcc gcttcagcta
     2881 tgaaaaaatg gatgcttctt ggaactttat caacaaaatc tggaatgctt cacgtttcgt
     2941 tatcatgaat atcgaaggaa tgaccgtaga agagatcgat ttatctggag aaaaatcagt
     3001 ggctgaccgg tggatcctta ctcgtttgaa tgaaacgatc gaacgagtaa ctgagctatt
     3061 tgatcgtttt gaatttggtg aagcaggtcg acaactgtat aactttatct gggatgattt
     3121 ttgtgactgg tacatcgaaa tgagtaaaga agtactttat ggagatgatg ctgtttcaaa
     3181 acaaaccacc cgtagcatct tagtccatac acttgatcag atcctacgtt tattgcatcc
     3241 aatcatgcca ttcgttacag aagaaatatg ggaacatatt cctcatcagg gaaattctct
     3301 agtagtggca gaatatcctg ttgtacgtcc agagtttgat gatgaaacag cttctaaagg
     3361 gatggaagta ttgaaagaat tgattcgagc cgttcgaaat attcgttcag aagtgaatac
     3421 accattgtct aaaccaatta cgttgctaat taaaacaaac gatgctaaaa tcgatcaatt
     3481 tttagttgaa aatacaagct atatcgaacg cttctgtaat cccgaagagt tgacgatatc
     3541 aagtgagatc actgcgccag atctagcgat gtcagctgtt ctgacaggtg cagaaatctt
     3601 cttgccgtta gcaggattga tcaatattga agaagaaatc aaacgattag aaaaagaact
     3661 ggaaaagtgg acaagcgaag taaaacgtgt ccaaggaaaa ctttctaatg aacgttttgt
     3721 ttctaacgcg ccggaagaag tagtagaggc agagcgtgca aaagaaaaag attatttaga
     3781 gaagcaaacg gctgtcaaag aacgaatcga aagtttgcgt actattcaat aagaatagaa
     3841 aaagagagtg tgaaatcagc tagctattac taaaagctag ctgattgaac atcgttacag
     3901 gaaagagttt gagcctcact cttagcttcg attcataagg agatgtgaca taaattttgt
     3961 cgcatctctt ttttagtcta ctcttattat gtaggagaag gagggaggtg gatcatgtat
     4021 caagagtcaa taaagtagtt gataaaatcg ttcctctctc tgtttctttt ctctttgtca
     4081 tacttttatt tttcttatgt gttctatttg ttataataaa agagaaatgg gggaattgtt
     4141 gatgacgatt gaagaagcaa tagaatggat ccatagtcga ttaccatttg gttcacgtcc
     4201 tggccttgac cgagtcgaag ctttattaga agtggtaggg catccagaga gaaaagtaaa
     4261 aacgatccat atcgctggaa caaacggaaa aggttctact gtagcttatt tgagaagcct
     4321 attagaagag gctgggctga cagtaggtac atttacttca ccttatatcg aaacgttcaa
     4381 tgaacggatc gcaatcaatg gcaaaagcat tccagacaat gatttgatcc agcttgtaaa
     4441 aaaatatcag ccactgatca aacaacttga tcaatttgaa gcagtagctg gtattactga
     4501 attcgagaca ttaacagcaa tggcatttga ttattttttg atgaagaaag tggacgtagc
     4561 gattatcgaa gttggcttag gcggcttatt ggattctacc aatgtagctg taccaatgct
     4621 gacgggaata acaacgattg gacttgatca taccgaaata cttggagata caattgaaga
     4681 aatcgctgca caaaaagctg gaatcatcaa acaaggcgtt ccagtagtaa caggaaatat
     4741 tgccccagaa gcttttactg tcatccaaca aacagctaaa gcaaaaaatg caccgcttta
     4801 tgtatacggg gaaaattata aaataaacta tctgcatcct gatgagatgt ggggagaagt
     4861 atttgaattt tttaatgaaa taggaaagat ccctcaatta accactccgc ttttagggca
     4921 acatcaagct gaaaatgcag gagtagccat cgaattgtat tttgtttact gtcaattgga
     4981 aaagctgcct ttccgagaaa aagatgtttt gaagggattg aagaaatctc gatggccagg
     5041 aagaatggag cgtttgagta acgagccatt agttgtttta gatggtgccc ataatacgca
     5101 tgcaatgaac cggcttgttg agaatttgaa aaaagaattc aaaggatatc aaatcaacat
     5161 tttgttttcc gccttacgaa ctaaaaatgt tagagaaatg ctagaacgat tgcagcaagt
     5221 accaaatgtc catatttatt tgacgacctt tgaacatcca aaagccatcg aattaagtga
     5281 agtatcagat ttagcaagtg agagagtatc aatcgtttca ttgtggcaat tcggattagc
     5341 agatatattg gaaaagatga caaatgaaga cgtattagtt gtgaccggtt cactttattt
     5401 catttcagaa gttcgccatt tattaataca attaggagga gatcatgaag tataatgcag
     5461 tgatttttga catggatggt ttgctgttcg acacagagtt agtgtattac gaagccacac
     5521 aagtagtagc agacaagatg ggttttcctt atgataaaga cctgtattta aaattcttag
     5581 gtgtttcaga tgaagaagta tgggcgaatt atcatatgat tttctcggaa tttggcaaaa
     5641 caagtgtaca aagattcata gatgattcat accaggaaac gcttgaacgt ttttcgcaag
     5701 gagcggtaca aatgaagccc ggtgtgttgg aactcctctc ttttttagaa gaacatcaga
     5761 ttcccaaagt tgtcgcttcc agtaaccaac gagcagtcat tgaactttta ttgaagaaga
     5821 acaatctgtt tgaacgattt gagacgattg tttctgcaga aaatgttcaa cgagcaaaac
     5881 cagacccaga aattttcttg ttagctaatc agaagctagg tacagcgaag aaagaaacat
     5941 tgattttaga agattcacaa aatggtattt tagcagcttc tgcggctgag atcccggtag
     6001 tcatggtacc agatctgctt ccaccatccc aagaattgat caaaaaaaca gctggggttg
     6061 tttcttcttt acatgaaatc cctgcattgt taaaataaaa atccatctct tttcgtatct
     6121 tattttagat aagatagaaa ggagatgttt ttttgggaaa acgattgatc aaagaagtac
     6181 ctactagctc tttaccaagg gaacgtatgg aaatttatgg cgcggaagct ttatcggatc
     6241 aggagctttt ggcaatctta ctgcgtaccg gtcaacaccc ttatagtgtg atgtcgattg
     6301 ctggaaatct gttaaaaacg tttggaggac tggcctcttt acgacaggct actttgcatg
     6361 agttagaaga gattcgaggg ataggacgcg tgaaagcaat cgaaatcaaa gccttgatcg
     6421 aattaggtcg acgaattcag acggatcatg aaagagcggc tccaccggtt cgtaccagct
     6481 acgaattggc ccagcaattg attctggaaa tgaaagatca caaacaagaa catttggttt
     6541 gtatttattt ggacacgaag aatcaagtga tcttaaaaaa aacagtgttc atcggttcgc
     6601 tgaatcagtc agtcgctcat cctagagaaa ttttccatta tgctgtacgt tattgtgcag
     6661 caaggatcgt tttagcgcat aatcatcctt ctggtaatgt tattccatcg cagcaggata
     6721 tgaattttac caaaaggata caaaagtgtg gagaaatgat ggggatcact gtactggacc
     6781 acttgatcat cggacgcaaa cgatatttta gtttgagaga agaaggaatg atggaagaga
     6841 agtaagttgc tttcttgcaa aaaaaatgaa gcaagtgtac aattatattg taattttgtt
     6901 tgggaataga agttgaaaca tctgttgcac ctctatcgta cacgagcaat gttacaattt
     6961 aaattagtgc ttttggcacg aggaggatat tattatggaa aacggaacag taaaatggtt
     7021 caacgctgaa aaaggatttg gatttatctc acgtgaagat ggaagcgacg tattcgttca
     7081 cttctcagct atccaaggtg acggattcaa aacattagaa gaaggtcaag cagtgacttt
     7141 cgacgttgaa gaatcagacc gcggcccaca agctgcaaac gttgtaaaag cttaatttta
     7201 tctgtaaatc aaattgatgg tagacatcaa tttttacgag agaacaaagc gagtaacaaa
     7261 aatacctgcc acaagagaca atttctcttg tggcaggtat ttttttgtat gaaaaagtaa
     7321 ataaatttaa gaggctatga aagagccacc ttgtatcaag gaataagaac aaacaaaaat
     7381 ggcttctcat gtttttcatt tgttttctct tatattcagg acaaggtgct gatttcataa
     7441 cctcttattt attcttcatc tggtgtcatt accattggca aaatcattgg atgtctttct
     7501 gtacgttcga ataagaaagg ctgaagcgcg ccagtcatcg cttctttcaa tttatattct
     7561 gaacagtcag gtccattcaa acattctctt aaagcgtgga ataagattcg ctgagcttca
     7621 ttgatcattt ctcctgattc tcgcatgtag acaaatccac gagagagaat atctggacca
     7681 gccaagattt cttttttctt gatatccact gtcgcgacag ctaatacaag accttcttct
     7741 gaaagcaggt gacggtcacg taaaacgaca ttaccaatat caccgatccc gctaccatcg
     7801 atatacacat ctccagccac aaaatgccca gcaggacgcg cagaatcgct agttaacgct
     7861 aaaacatcgc cattttccat gatgaagcag ttttctgcag gaacacctac atcttgtgct
     7921 agagaagcat gtactttcaa catgcggaat tctccgtgga caggaataaa atattttggc
     7981 ttcatcaaac gaagcattaa tttttgttct tcttgtccac cgtgaccgga agtatggatg
     8041 ttattgattt ttccgtgaat cacttctgca ccagcttcag acagcaaatt gatcaaatga
     8101 ttgacacttg ttgtattacc aggaataggt gaacttgaaa agatcactgt atcgtctggc
     8161 tggatttgga tttgacggtg agtaccattc gcgatgcggc tcaacgcggc catcgcttct
     8221 ccttgagaac ctgtacatag gatcatcgtt tcattggcag gcaagcgatt gatttcgtgt
     8281 gcatctacaa atgtgccgtc tggaactttg atatatccta aacgttgacc gttgacgatg
     8341 gcattttcca tgcttcgtcc gaaaacagcg attttacgac cagttgccac agcggcatct
     8401 gctgcttgct gcaaacggaa gatgttagaa gcaaagctgg caaaaatgat acgtccttcg
     8461 atttttgaaa agatcttcaa aatagaatgc ccaatcgttt tttctgattt agtaaatgtt
     8521 ggaatctcag catttgtact atcagataaa aggcacaaga ctccttcttc cccgatacga
     8581 gccattttat gcaagtttgc tggttcgcca acaggagtga aatcgaattt gaagtctcca
     8641 gtcgctacga tatttccaga aggtgttttc accacgatcc ctaatgcatc tgggatacta
     8701 tgggtcgttc tgaaaaagct gacagaagtc tttctaaaac ggatgatcgt atcttcattg
     8761 atctcatgga gctctgcatc tcgaagcaag ccatgctcat ctaatttgtt tgtgatcaac
     8821 gctaatgcga gaggaccagc atagatagga atatttgctt gtttcagcaa gtagggtatt
     8881 ccgccgatgt ggtcttcgtg cccgtgagta atcaccaagg cttttacttt gtgtaagttt
     8941 tgtatgatgt agctgtagtc gggaatgaca tagtcaattc caagtaaatc gtcttccggg
     9001 aatttgatcc cggcatcaat caaaatgatt tcatcttgga attgtacccc atacgtattt
     9061 ttcccgattt cacctaatcc accaatagca aagacgcctg tctcgttatt tttgatggaa
     9121 agtttcatat taaaactcca ctaatgagaa atcagcattt tctttttcgt actctagatg
     9181 acttccttct agaagttgaa cgaattcaat attgtatggg gtattttctt ccacacgttg
     9241 gcgagcgtct acatcgcttt ttgcctcaat gtaaagagat tttgtttctt cccgtttagg
     9301 gttgcgtact tttgtttctt gataataaac tttatagatc attgttttgt ctcctttatc
     9361 tgcgtaaagc agtgatttag ctagttgatt tttttagaca acacaaaacg aggggacaag
     9421 ccacctggtt tttcatatgc tttgactaga atactggatt cgagtcaaag agacaagcct
     9481 cgtttttgct tatctgttcg gatatttttt caaccgtacc ttctgtataa aaaatataca
     9541 acaattaatt gttattctag cataaaacag caaataagta tagaaatcaa ctagattgaa
     9601 agaaaaaatc aatgtttatc atcccgctat aatcataatg acttttttat gagagaagtg
     9661 ttctattaat aggctcgttg tgctatcatt tttagtaatg aaaatgtaac aaaagattaa
     9721 aaggagatga gaaagtgaaa ctaatgtggc attataccat gcgttataag aaattcttgt
     9781 ttttcaattt catatgcgta tttggtttta ttttgattga attaggactt cctacgattt
     9841 tagcacgtat gatcgatgta ggaatccgca atgatgattt tgattatgtg aagcaacaag
     9901 ggatatttat gatcgttatt actgtaatcg gtattatttt gaatatcttg ttaggttatt
     9961 ttggttcgag gattacgact aatatcgtag cagatattcg agatgatttg ttcgaaaaga
    10021 tccaaagtta ttcgcatcag gaatacgaaa atctaggcgt atcttcattg atcactcgta
    10081 caacaaacga tgcttatcag atcatgttgt tcctgcaaaa tattttacgt atcggtttta
    10141 tggcaccaat gatgttcatt gtcagtttgt acatggtcat gcgtacaagt gtgacactca
    10201 gtttgtatgt agtaggtgca ttacctttac tattattggc agtcgtagcg attgctaagg
    10261 tgtctgaacc gttatctaaa aagcaacaaa aaaacttaga taaaatcaac agtatcttgc
    10321 gggaaaatct ttcaggattg cgtgtgattc gtgcattcgt caatgaaaag tttgaagaaa
    10381 aacgttttgc tgtagtcaat gagaattatg cttctagttc aaaaagctta ttccgtttga
    10441 tggctgttgc acaaccgggt ttttatttct tatttaatat cgtaatggta ttgatcatct
    10501 ggagcggggc agtgcaaatc gatgcaggcg agcttcgtgt aggagatttg attgcgttta
    10561 tcgagtatat attccatgcc ttgttctcat tcatgttatt tgctagtgtg ttcatgatgt
    10621 atccaagagc agctgtttca gctggacgta tccaagaagc cctagatgcc aaaccttcga
    10681 ttgtagaaga tccaaatggt atatctgata caaaaactaa agggtatatc gaatttcaaa
    10741 atgtaacatt tgcttatcca ggccattcgc aagagccagt gatacgaaat gtcagtttta
    10801 cagcctctcc aggagagacc gttgctttta ttggaagtac cggtagcgga aaatctacgt
    10861 tgatccaatt gatccctcgc ttttatgatg tgagtgaagg aaagatcttg atcgatggag
    10921 tagacgtgag agattatcgg ctaagcaagt tgcgagataa gattgggttt attccgcaaa
    10981 aagcgctgct atttactgga acgattgcag ataatttgcg atacgggaaa gaagatgcta
    11041 cgcaagaaga aatggaacgt gcagctgaga ttgctcaagc ttcagaattc atttctcgaa
    11101 aaccagatgg atttaatgaa catttatcag aaggtggttc caatttttca ggtggacaaa
    11161 aacaacgatt ggcaatagcc agagccatca tacgccgtcc ggaaatctat atttttgatg
    11221 acagcttttc tgcattagat tatcaaacgg atgcgaagtt acgtgctcgt ctgaaaaaag
    11281 aaacgacaga atctactgta ttgatcgtag cacaacgtgt gggaacgatc atgcatgcag
    11341 ataagatcat cgtattaaat gaaggagaag tcgtcggtat ggggacgcat cgggagttgt
    11401 tggaaaaatg cccgatttat tatgatattg ccgcttctca attatcaaag gaggaattag
    11461 catgaaaaaa accttttctt ccttaaaacg tttagctcga tatatcagac cgtatcgtga
    11521 gacttttctt ttagtgatcc tatttacagt tctgacagtg gctttcaacg tggctttgcc
    11581 atatgttaca ggacttccta ctacagaaat cagccgtaat attgccaatg gcgaatcaat
    11641 caatttttct tatatattca aatgtctcat ttggatcaca gttgttggaa tagggtattg
    11701 tatttcgcaa ttactttctg gtgttttgat gacaaatgtc gttcaaagtg ccatgcatga
    11761 tttgagaaga gacatagaag aaaaaatcaa tcgtcttccg gtttcatatt tcgataaaaa
    11821 ccagcaagga aatattttgt ctcgtgtgac gaatgatgta gatgcagtca gcggtgcttt
    11881 acaacaagct tttattggta tcgtaaatgc gatattggga attacgttgg ctgcagcaat
    11941 gatgttttat atccagccga tgatggcgct gatctctatg atcatgattc caacatcgat
    12001 ctggatctca aaacgagtga tcaatgcttc gcagaaatat tttcaaagca tgcagaattc
    12061 tcttggtgag ctaaacggct acgtccaaga aaatatgaca ggttttagtg tattgaaagt
    12121 atatggacgc gaaaaagaaa cattggaagg attcaaaaaa gttaatcaca gcttgaaata
    12181 ctttgggttt cgtgcagcat tcatttctgg cttgatgatg cctctagttc aattaactgc
    12241 ctatgctact tatattggta tggctgttct aggaagcttt tatgttatta ctggggcgat
    12301 cgttgtcggt caactacaag cattcatcca atatatttgg caaatcagtc agccaatggg
    12361 gaacgtgaca caattgtctt cagttttgca aagtgcatca gcggcaacga aacgtgtatt
    12421 cgagatttta gatgagccag aagaaaaaga aaacaaagta gatgcttcac tgccagaaac
    12481 gattttagga gatgtgacat tcgatcatgt cgattttgca tatgatccga agaaaccatt
    12541 aatccaagat ttgaattttg aagtgaaagc tggtcaaacc gtagctgtgg tcggtccgac
    12601 cggtgccggc aagacgacat tgatcaatct attgatgcgt ttttatgacg tagacaaagg
    12661 agcaataagg attgatggaa tcgacaccaa atcaatgagt cgcagtgatg tgcgttcgct
    12721 atttggtatg gtcttgcaag atgcatggct ttatcaagga acgattgcag ataatatccg
    12781 cttcggaaag cttgatgcga cagattatga agtagttgat gctgcgaaaa cagcaaacgt
    12841 agatcatttt atccgaacga tgccagatgg atatgatatg gccatcaatt ctgagggaga
    12901 caatgtgtct ttaggacaaa aacagttgct gacgattgcc cgagcagtgg tctccgatcc
    12961 taaaatcttg attttagatg aagcaacaag ttcagtagac acccgtttag aagcgttgat
    13021 tcaaaaagca atggatcgcg tgatggaggg acggacaagt ttcgtcatcg ctcaccgttt
    13081 atcgacaatc cgtgatgcag atctgattct agtgatggat caaggtcaga tcattgaaaa
    13141 aggaacgcat gagagtttat tggcacaagg tggattttat gaaaaactgt ataacagcca
    13201 atttgctgag gaagcagaat aagtcatata agaataaaac agtttaaaac ttattttttg
    13261 ttctctattc ttgataatgg taaagtaaag ttatcaagaa aagtgaggac acggagatgg
    13321 aacagttttt aaatgaaaaa attactgaag ccaaaagaga agttgatcgt ttaacgcacg
    13381 tctgtcagac gaagcctttt gattcagctg atttttttga aaatgagttt caaatcgaac
    13441 gcttggcagc acaaatagaa gtgtacgaat taattttaaa aaaagtaata gaagaaaata
    13501 tagatgtaca ataaaaacag gtaaaacgtc agttaccaga tactgtaaat agacatctgg
    13561 cctgagcagg ttttacctgt tttttttctt atgaaataaa tatatgctat gataccttta
    13621 ttcaatagga ttagtttcta caaaaaatga aaggagaaaa ataaatatgg aacggtttat
    13681 agcatcaaat gccacagtga ttggtgatgt gacattgtca gaagatgtga cgatctggta
    13741 tcaagctgtt ttacgcgggg acagtaattg gataaaaata ggacaaagaa cgaatatcca
    13801 agatggtaca atcatccatg tcgatcatga tgcacctgta gatattgcgg agaatgtgac
    13861 ggtaggacat cagtgcatgc tacatggctg tacaatcgaa aaaggggcgc tgatcgggat
    13921 ggggaccacg atattgaatc atgcggttat tggtgagaat agtttgattg gtgctggatc
    13981 attagtaaca gaaggaaaag ttattcctcc taatgtcttg gcttttggtc gcccagctaa
    14041 agtgatccgt cctttgacaa aagaagaaat acaaaaaaac aaagaaaaca tccagcatta
    14101 tgtcgagata ggtcggatgc atgcgcaagg aaaattcaag gagcaaagat aggcagtagc
    14161 atccataaaa taaaaaagga tggtcagttt gagttgacca tcctttttta tttggtttag
    14221 ataagagagc ttaaaaaagc ttcctttgaa acagattaat ttaatgaaga actgttttcc
    14281 atgacttcat cgatcaatcc atatgctttt gcttgttcag ctgtcatata gttgtcacga
    14341 tctgtatctt tttcaatcac ttcaagcggt tgtccagtgc gttcagctaa aattttgttt
    14401 aagcgttcgc gtgtttgcaa aatatggcgt gccgcaattt cgatttcagt tgcttgccct
    14461 tgagcgccgc caagaggttg atggatcatg atctcagcat ttggtaaagc aaatcgttta
    14521 ccttttgtac cagctgtcag caagaagctt cccattgaag cagccatacc cataacaatt
    14581 gtttggacat ctgctttgac aaagttcatt gtgtcgtaaa tagccatacc tgctgtaact
    14641 gaaccaccag gagaattgat ataaagatag atatcttttt cagaatcttg agcgtctaag
    14701 aaaagcaatt gggcgatgat cgaattagca agatcatctg ttacttggcc gcttaacatg
    14761 atgatacggt ctttcaataa acgtgagtag atatcgtaag ctctttcacc tcgtgaagat
    14821 tgttcaatga ctgtaggaat taaattcata atagagttcc tccttgattt cgtttagaaa
    14881 tagtgtacca taatttagtt tctaatacct aaaatgtagt atacattttt ggtcaaaaaa
    14941 ggtcaaataa aaaacttagg caaattagtc gcataaaccc gctttatggt atgataagca
    15001 gagaaacaaa acaaggagga acaacagatt gatagttggt attactggcc ttatcggagt
    15061 gctgacaggt gctgtagttg tctatatcgg catgatactg caaatgcgtc acttggaaaa
    15121 taaagaacag cgtagacgtg aattggaatt gaaagtcaaa gaaatcgaga cgctgaatca
    15181 attaaataaa aagatcaacg agatcttgca aaaacggaca acgctgatgg aagaatatgt
    15241 cagctttgat gcatttgatg attgttatat cacgattgac gattttgcgt atctccaatc
    15301 attttcagcg caaaatagtt tttatctgcc aaattatatt ttagacgagc tatttaagaa
    15361 tatctcccat cgaaaagtga ttttatctcc agatgaaaca atgaagattg gtggatatgc
    15421 ttataaaggc ggacgcgtga ttttagagaa ttttacggat agtctgactg aaatgatcaa
    15481 tgaaaaaaaa gctcagttga agactatgac gcgccagccg attggtttct tctcgaaaga
    15541 tgatgtttga agctgatatt ttgaagcatt gccgttgcac atgagagcgt ggtaatgctt
    15601 tctttttttc ttaatgatac aatagaactg aacggagtga agaaagatgt tacaaatcta
    15661 tgcccatcgc ggaagcagcg gcacccaccc agaaaatacg cttcctgcat ttgcagaagc
    15721 tgtacgagta ggtgcagatg gtatcgaact agatgtccat ctaagcaaag atggttattt
    15781 gatcgttatg cacgacgaag aagtggaccg gacgactaac ggtaaagggc tgattcgaga
    15841 aaaaacattg gaagaactca aaaaattgaa tgcagggggc aaattcagca aagaatttcc
    15901 agcagctaaa gttcctgaat tgagagaagt gttaggactg ctcctccaaa aaaattaccg
    15961 agggatgcta atgattgaaa ttaaaactga tcagtatgaa tatcccggta tcgaagaaaa
    16021 attagccgaa acattaaaaa gtagagattg gccttttcaa catgcatatt gcagctttca
    16081 tttggattct ttggaaaaaa tcgcattact ggaaccggag atacagctgg acttattgat
    16141 gagtacttct gataaaaaga tccaactagc agcagaaacc ccttatatag aaggagtaca
    16201 tccaaaaaat acttggttgt tcgaacatcc agtccaggca gctgattttc caaaagcaat
    16261 tcgtgcatgg acgattaacg atgaagaaga aatggccaaa tgtcaagaac tcaaaataga
    16321 tggaataata actgattttc cggagaaagc tcttcggtgg aaacaatcaa cgaaaagtag
    16381 gtaaaatatg gaaaaagtat tagtgatcgt cggtccgaca gcagtcggta aaacagcgct
    16441 aagtgtggaa ctcgcacaac tatttggagg agagatcatc agtggtgatt cattgcaaat
    16501 ctataaaaat ctggatatcg gtacagcaaa aatcaaagaa gaagagatga ataatgttcc
    16561 gcatcatttg attgatgtga tcgaacctac tcaacgttac tcagccgctg attttcagca
    16621 ttcaggtcga caattgatta ccgagattgc gaaccgaggg cacctaccta tcatagctgg
    16681 tggtacgggt ctctatatcc aatcccttct atatgattat cagttaggag cgacagaaga
    16741 agcgaatcct gagattagaa aaaagtatga aacgttagct gaaacaattg gttcccaagc
    16801 attatggaat cagttgaaag aaaaagaccc ggcagctgct gagaaaatcc actggaacaa
    16861 tcaacgcaaa gtgataagag ctctggaagt tttagagacg acaggacata gtatcacggc
    16921 acctaaagaa gagccaaagc aattgtatga ttacctcatg attggattag atacgaagcg
    16981 tagtattctt tatgaacgaa tcaatcaaag agtcgatttg atgctagcag aaggtttagt
    17041 agcagaagcg cgtaaggtgt atgagcttgg agaagtccag gccagtcaag gtattggata
    17101 taaagaattt ttcccttatt ttgaagggaa agaaacattg gaagaagcaa cagaacaagt
    17161 caaactacat tcgagaagat acgcgaaacg tcagttgacg tggtttcgta accgtttgga
    17221 tgcacagtgg tttgatctta taagtgagcc aaatcaaaaa agtcagattg agcaaacaat
    17281 caaaaaatgg ctggaggcgg aataaaatgg aagaaaaagt gatcatcgta ggggtagaaa
    17341 ccgaagcaaa ccagcgttac ttttcagagt caatggaaga gctagttcag ctgacgaata
    17401 ccgcatcagg agaagtcgta ttcacaatta cacaaaagag accacaagtg gatcggcaaa
    17461 cgattatcgg taaagggaaa ttacaagaat tagttcaaca agcggatgcg catgaagcgg
    17521 atttgatcat ctttaatcat gagctaactc cgagacagag ccaactgatt accgatgctg
    17581 ttggtcttcc ggtaattgat cgcgtgcagc ttatcttaga tatttttgcg atgcgtgctc
    17641 gttcgaaaga agggaagctg caggttgaac ttgctcagtt agaatacctg ctgcctcgtt
    17701 tagtagggca agggaaaaca ttatctcgtc ttggcggtgg gatcggaaca agagggccag
    17761 gtgaaacaaa gttagaaaca gaccgccgac atatacggaa taaaattttt gctgttaaga
    17821 aagaattgaa agaagtagaa gctcatcgag agcgcaatcg ccaaaaaaga aaaaatagcg
    17881 agatttttca gattggactt attggctaca cgaatgccgg gaagtcaact atcttgaatc
    17941 tattgacaca agcggatact tattcgaaag atcaattgtt tgcaacgtta gatccattga
    18001 ctaagagatg gcgttttgct gaaggatttg agattgctgt aacggatacc gtcgggttca
    18061 tccaagacct gcctacgcaa ctgatcgatg ctttccattc tacattagaa gaaagtcaaa
    18121 atatggatct tttgctacat gtagtcgatg ctagttctcc agatcgtatt ttacaggagc
    18181 agactgtctt aaaattgatg gatgaactga atatgaaaga aatgccgata ctgactgttt
    18241 acaataaggc agaccagatc gacccagcga tgttcacgcc tacgctattc ccaaatgtat
    18301 tgatttctac tcagacgaaa gaaggtaaga tggcgttgat cgaagcaatc aaacgtcagc
    18361 tgatggaact aatgacccct tatacacttt ctgtgccaag caacgaagga cagaaattaa
    18421 gcgagttgag aagacaaaca atggttttgg acgaagcgta catggaagaa acaaacgaat
    18481 atgaagttaa agggtttgca aaaaccaatt caaaatggtt aagaaaaaac caataaaatt
    18541 ttattttttt aa
//
LOCUS       DAZUKC010000049        18061 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000049 DAZUKC010000000
VERSION     DAZUKC010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.050"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            65..940
                     /locus_tag="ACG80T_001714"
     CDS             65..940
                     /locus_tag="ACG80T_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFS4841353.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1085..1237)
                     /locus_tag="ACG80T_001715"
                     /pseudo
     CDS             complement(1085..1237)
                     /locus_tag="ACG80T_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1505..2836)
                     /locus_tag="ACG80T_001716"
     CDS             complement(1505..2836)
                     /locus_tag="ACG80T_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4841354.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(2985..3698)
                     /locus_tag="ACG80T_001717"
     CDS             complement(2985..3698)
                     /locus_tag="ACG80T_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4841355.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            3871..5295
                     /locus_tag="ACG80T_001718"
     CDS             3871..5295
                     /locus_tag="ACG80T_001718"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4841356.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            5594..6301
                     /locus_tag="ACG80T_001719"
     CDS             5594..6301
                     /locus_tag="ACG80T_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFS4841357.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            6288..6611
                     /locus_tag="ACG80T_001720"
     CDS             6288..6611
                     /locus_tag="ACG80T_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFS4841358.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(6700..7737)
                     /locus_tag="ACG80T_001721"
     CDS             complement(6700..7737)
                     /locus_tag="ACG80T_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS4841359.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(8058..9041)
                     /locus_tag="ACG80T_001722"
     CDS             complement(8058..9041)
                     /locus_tag="ACG80T_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFS4841360.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(9456..10226)
                     /locus_tag="ACG80T_001723"
     CDS             complement(9456..10226)
                     /locus_tag="ACG80T_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4841361.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMIFGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(10219..11154)
                     /locus_tag="ACG80T_001724"
     CDS             complement(10219..11154)
                     /locus_tag="ACG80T_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4841362.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(11470..13119)
                     /locus_tag="ACG80T_001725"
     CDS             complement(11470..13119)
                     /locus_tag="ACG80T_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFS4841363.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(13236..15839)
                     /locus_tag="ACG80T_001726"
     CDS             complement(13236..15839)
                     /locus_tag="ACG80T_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFS4841364.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(15851..16901)
                     /locus_tag="ACG80T_001727"
                     /pseudo
     CDS             complement(15851..16901)
                     /locus_tag="ACG80T_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(16927..17241)
                     /locus_tag="ACG80T_001728"
     CDS             complement(16927..17241)
                     /locus_tag="ACG80T_001728"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFS4841365.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(17257..17703)
                     /locus_tag="ACG80T_001729"
     CDS             complement(17257..17703)
                     /locus_tag="ACG80T_001729"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFS4841366.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(17704..>18061)
                     /locus_tag="ACG80T_001730"
     CDS             complement(17704..>18061)
                     /locus_tag="ACG80T_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4841367.1"
                     /translation="IITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
BASE COUNT         5514 a         3659 c         2984 g         5904 t
ORIGIN      
        1 tacttttttc ttaaacgttt ctttatacta aatataaata agtgtttcat gaaaggagaa
       61 ctagatgaat aattggcttg agtattttcc tgaaaacgtt ttggaacgcg gttacagcta
      121 tcatctgcac ggttttgtcc gtcatttgaa ctatacaagc aagtacttgt ccgcaacagt
      181 ttctggcacg gaagattata aggtagtgat cacttgggat gaaaagacca acatgacttg
      241 tgattgtcta tatgccatag aaggaaaaaa atgcaaacac atggctgctg tcttattcgc
      301 ttacgaagaa cgtccaataa aaaaatccaa ctactcactt tccgaactct cgtcccttgt
      361 ttcctccgct agttcctctc ttgtacggga gcttttaaca gagattctta tagaacatcc
      421 gcaatttatc gaacgcttca aagttaagat gccatttcat gcaataaatt attcagataa
      481 attaacaact atcatccata aatacgatca tattatcaag aaaaacaaaa acaggaaaac
      541 agcgaagttc atcatggaga tgcgaaagtt catccaagaa gcagttgaat cgctcattca
      601 gcaaaatgcc tatcttcccg cattcgaatt gatcaatgaa gtgattgcta ctctcgaaac
      661 attctattgg gagcctgagg atgaacgaac ccttcttctg atagaggact gttattattt
      721 atggaaagaa ttattagcag aagcacctca tgcagaaaaa aggcaaatgt tctcttggtt
      781 cgtctgccaa gtcgatcata ctgacgcctc ttattcaaaa cgctactcca taaaaatcct
      841 gaaagaagac tttcgggaaa aagaattttc taaccaaaag aaaaaaatag ataaaaaaac
      901 taaagaaacg gtaaagaaag acgatttcaa tgaaaaatag caagtaaacg ataacaacca
      961 tcctaaatcc acaagaaata cagcagacct tgctttaaaa acacgaatca tgcaaaggat
     1021 gaagtaaaga agacttaacg cgtacccaat attagtcatt tgactattag gtacgcgttt
     1081 tgttttataa gtcatgacaa atttccttcg ttgctgggta gattttccgg cattgttgat
     1141 agattttatg atacttttct acattttgta agagaggttc cactgcatct gtatattgga
     1201 taaaattctg acaacaactt tcaggtgtgt cgaaccactc taatctttaa agataaatat
     1261 taaaataacg attaataata ttaacagtct ttatttttca ccaccaaaaa cctatctttg
     1321 catatactaa atagacgtat atacacagat aggtttgtct tgtgaaaaat aatgattatc
     1381 cttaccaaaa aatgtttgtt tttctacatg ttcgtactta tcaaaaaata tgatccttat
     1441 tgaaattact ggctatttat attccaacga cttcttgtgt taatttttgt tatctcatag
     1501 taaattatgt ttcattttca cttaatttag ttgtcacgtg ttcgtcaatt cgtaaaaacg
     1561 gtaaatatat aaacacactt gcggctataa ttaatatttg taaaacagct gctcgccagt
     1621 ctccggctgt tgctaaaaat cccgatataa ctggtggcgt agtccaagga atttgcacta
     1681 ctacatgatt aattaatccc acagcagttg caaaatatgc tactgtcaaa gaaaaaatcg
     1741 gtgcaagcac aaagggaata atcagactga tattaaatac aataggcaat ccaaaaataa
     1801 taggttcgct tatattgaat atagcgggag ttaccgataa tttagcaaag tccttataat
     1861 cttttctacg actgaagata aatattgcaa tcaataaagc aatcgtattt cccgatccac
     1921 ccattaccgc aaaggtatct ttaaaggcca tattgataat atgtggaatt tgctctttgt
     1981 ttgcataagc tgccatattt tcttgcatat tctgaatcaa aaacggatct aacaatgaac
     2041 ctgatattac tgcttgatgg ataccaaatc caaaaaatag attagcaata gatgtaatta
     2101 acaaaaatcc tggtaaacca gttgtaattt tacttaatgg ctctgtaact atcgtggtaa
     2161 ttagcttatt tatatccata ttaaacaatt gaatgattat aaatgacaat aaagcaaacg
     2221 aggtaatcgt aataatgatt gggattaata cattaaaaga ctttaaaaca gcgggaggaa
     2281 tatttccgga tagctgaatc tgtagtttct tattattaga aagtttaata aataattctg
     2341 tagccgctaa tccaacaaat atacctacaa acattcctga agctcctgta taggatatag
     2401 gtatcacacc cgtcacttca acagctttat ccgcattttc aggagtaaac atcatctgca
     2461 taggtgttaa tacaacaaat gtcgaaatag caactaatac cgctgcaata tgatttgtat
     2521 aatttctgct tgcacacata tgataagata ctgctgctac aactaataat gtagttatgc
     2581 ttaatgtacc attggcaatt gaagtaccaa tttgttgtaa cgtggtcaat gtactagtat
     2641 cgacaatttc ccccatgaat cctgttggtt ctaagatcac tgaattaata agtgtcacaa
     2701 atcctgctaa catcataaat ggcataaatg tagcaaacgc atctcgcaaa gttcttaaat
     2761 ggatctgatt ccccaactta gtcgcaacta taccaaattt ttcaaataca atgtccttcc
     2821 tgctactttt gcccatattt tttctccttt atgcttattg taatcgatta ctttttccac
     2881 ccaaataata catcacttat caaaaaaagt aaatactttt aattaaatat atatatcttt
     2941 tttagtataa aaaaataagc tctgaaaaaa taattttccc gagcttattt ttcaacaaaa
     3001 acagcttcaa aaacaaagtc ttgatataaa tgtctcgata ccgaatactc gaaaggttta
     3061 ccagacgata gagcaacaac tttgtctatc tcaatcataa attcacctgg atctattttt
     3121 aatatttttg cgtcaaattc attcgcagta tctcctttga tacgtatgtg cgaagtcttt
     3181 atttccagac ccagttcatt tgtgatataa gcatatacag atttctttaa atgttttggt
     3241 tccaagcctg gtatgactgc taatggcatg aatgtttgct caatcgagta tggttgacaa
     3301 tcaataaaac ggcttcgtac aatattatat acatagtctt ttacactgat acttaattta
     3361 tcagctactt ttttcgatgg aagttcaatt gagaaagtat ggatttctga atctacgtgt
     3421 tcttcaccaa ccacgctaaa caaaccttcg tttggaccaa gaactcgtga gttatcaatt
     3481 ggtgttctga cgacttcagt cccataccca cttctacttc gcaaaactcc ttcggaaaca
     3541 agtaagtcta cgcctttctt tactgttaac ttgcttgtat gataaataac tgccaattca
     3601 tctcctgtag gaagtttctc accaatttta tactctccat ttacaatttt tttctttata
     3661 tctaagtaaa tttccatata cttcgcaatt tttttcaatg gtgaccctcc aatcaaaaac
     3721 atatcttcat ctatacttta acataaataa accgataaaa tccacatcaa aattatacac
     3781 acaataaaaa agatatatat ataaacaaaa ctatttactt taagttgtaa gcggtgttat
     3841 cattaagtgg aaacaggagg taaaaaaatt atgacaacta tgaaaaagct aacttttcca
     3901 gatgattttt ggtggggttc cgcttggtca gctgagcaag cagaaggaaa aggagaaaca
     3961 ggaaaagcag agactatctg ggatcgctgg tttaaagaac aaccttaccg gttttatcaa
     4021 cgaatcgatt cctctattac aaccgatcac atccatcaat ataaagaaga tatagaacta
     4081 atgaaaaaaa ctgggcataa ctcctttcgc gtttctattt cttgggcacg aatgtttcca
     4141 aatggcggta ccggaaaagt aaatcccaaa gccatttctt tctatcgaaa attattttcc
     4201 gaaatgaata ataacggaat caaagtattt gccaacttat atcattttga catgcctgct
     4261 actcttcaag ataaaggagg atgggaatca cgtgaagtag ttgatgctta tgttcattac
     4321 gctgaaactt gttttaaaga atttggtgac ttagtttgtc attggtttac cttcaatgaa
     4381 ccacttgggc ctattttagg tacttatctt gaagattttc attatccaaa cctcattgac
     4441 tttaaacgcg gagcgcaagc agcattcttc actatacttg cacacgcaaa agcaatacaa
     4501 gtatttaaag aactagctct tcctagtaaa ataggcgtta ttctaaattt gagtcctact
     4561 tttcctcgta gtcaaaatcc tgctgatgtg aaagcagcag aaatcgctga tttattttat
     4621 acgcggagct ttttagatcc tatggttaaa gggaattttt ctaaaaagct tatcctgtta
     4681 ttacaagaat ataatcagct tcctgaagat tatacgaaag aagatcttgt atgtatctac
     4741 gaaaataccg ctcaaattct tggactaaac tattacgagc cacgtcgcgt caaatctcgt
     4801 tcttctatgg tggatacatc tagtccattt ttaccagagt ggtttttcga accacatatc
     4861 atgcctggac gacgaatgaa cgaataccgg ggatgggaaa tttacgaaaa aggaatttat
     4921 gatttgtgca tggatatcaa gaaccattat ggaaacatcg aagcatttat ctcggagaac
     4981 ggaatgggcg ttgcggatga agaacgtttc atggatgata cagggcaaat aattgatgat
     5041 tatcggattg agtatatcaa agatcatctt gcttacttat ggaaggcaat aaatgaagga
     5101 tgtaacatta aggggtatca tctttggaca tttattgatt gctggtcatg gatcaacgcc
     5161 tataaaaacc gatatgggct ggtttcactc gaccttacta ctcaaaaaag aacaattaaa
     5221 aaaagtggcg aattttacaa gcaattaagt gatacaaatg gatttagata cgatgtagac
     5281 ttgttagttg aataatattc taaaattcca cttgaagtga acacattaga aaacaagaat
     5341 aatgagaaat accaataaca aatacttttt cttcatactt catttatact tgtcagtata
     5401 agagactgtg acaaatgtag tgatcctcag aagttaggtc tggaaatcta acttgagaag
     5461 atttttttaa actacgtcca gtctttttta tttgtttaaa attgaagttg acaagtcttc
     5521 attaaaaatt tagaatattc acaagtttga cgacaattat gaaaatttat gtcgtgctaa
     5581 aggagttttg ttattggatg aaaagttaga tataaaaact gcgatcaaag acacgttacc
     5641 taccgttttt ggttatatcg gtattggact tgcatttggt atcgttggga aagctgccgg
     5701 gtttcatccg ctggtcgtca cgttgatgtc cctgctgatc tatgctggtt ctgcccaatt
     5761 tatcacggtc agcatgcttg ctagccatag tccactgctc tccatcgttc tctcgacctt
     5821 tctagtcaat tcccgaatga ttctgatgag tatgacgatt gctccttatt tcaagaaaaa
     5881 tcggctgctt caaaatctgc tgattggcac attgctgaca gatgaaagtt tcgcactggg
     5941 aatgaataaa ttgaactata ctggacaaaa gttaaacttt cgttggatga atacagctaa
     6001 ttggatttct tatttgacgt gggtgggttc ttctcttgtt ggcgcacttt tagggaattt
     6061 tattactgat ccgaaaaagt ttggtttgga tttcgccatc gtcgctatgt ttatcgggtt
     6121 actttatctt caagtcattt ctgatagaaa cacctctaaa cgattacagt tgatcctgat
     6181 tggtctcact ttgattctgg tatatgtcgg actgattttt atcccaagta acttagtgat
     6241 tttagttgta acactgattg ggtgcggatt gggggtgtgg atcaaacatg ccttcttttg
     6301 agtttacatt gctgactatt ttaggttgta ctttagcaac ttggctttct aggatcctac
     6361 catttgtctt actgaaaaaa ttcgatttgc cgcaaccgtt gcttgagtat ttaagttttg
     6421 tcccgatcgt cattatgtct gctttatggt tcagcagtct atttacccaa aatatcggac
     6481 atctgccgca gatcaatctc gaaaatgctt tggcttcagt tcctacactt ttagctgcta
     6541 ttctttcaaa aagcttattg gtgatcgtct tggtaggaat tctttccctt tcgttgattc
     6601 gcttattata aataaaaaaa caagagctta tggcaaatta ctgtcatgcg ctcttgtttt
     6661 tatcaaagtc aaataagaaa aaaatcggcg tattcttgct tatccgttga acggaccact
     6721 aacaacgact tttcctaata tatgtcctgt ttctacgtct ttggttgctt gtttcaagtt
     6781 cttcgcattc atcccatctg aatagacctt gctcaatgta ctgcggataa tcccttcatt
     6841 tgctaaagca gcaatgtgcg ctaaagcttc tccttgcgtt tcgatttcat agttatagtc
     6901 tgttttagcg aacatatact cccagtcgaa gctgatagat tggtttttcc aaacacctat
     6961 atctaacggc tctttgatcc ctacgatcgt tcccacatgt ccaaatggtc ggacaaggtg
     7021 tttgatttga tcaaagtagc gggtaatatc gaataataca gcgacatact ctacttgatc
     7081 gattcctaat tcacgaaaac ttttacttaa gtcgtgatga taatcgattg gatagtctac
     7141 acctgttttt ttcagccatt caaaattctt cggacttgca gtcgcgtaga cttctaggcc
     7201 agcccaatgc gctagttgat tcaaaatcga accgacgccc ccagctccgt tgatcactaa
     7261 gatttttttc ccgcggttgg cattttcttc tggagtcaga ccaaattttt caaataacaa
     7321 ttcataagcg gtcaatgagg tcaacggcaa ggctgcggct tcttcatctg aaagattttt
     7381 tggtgcaaga gcgacaatac gttcgtcaac cagttggtat tcttgattgc ttcctgctct
     7441 tgtcgttgta cctgcgtaaa aaacgcgatc gccgacagaa aatttttgca cttcatcacc
     7501 tacagcaaca actttcccga ctccatcaaa ccctaagata cgtaaagcat ttcttatccc
     7561 gttatcttgg cgaagtttcg tatcgacagg attaacagat actgcgttga cttttaccag
     7621 aagatcccgt gaactgggta caggtatctg ttctttttca tcaataaagc tttttggatc
     7681 atcgatcgac aacccttcat aaaaaccgac taccttcatt aattcctttt ctgacatacg
     7741 aattcctcct tagcttcatc aagttggagc atccatacag ttttcatttc aatatatata
     7801 gtatgctatt tgttccactt ttattcatca taattattct cttacttaaa gtaaactatt
     7861 tgcacttttt tggttaaaaa agaggttgcg acaagacttt tgtcacaacc tcttttaaac
     7921 ggtgttcaat caactgaccc tcggtattcg ctcaaaaaac gacaaaatat gaggagctat
     7981 ttttgtttgg ctgttcttat tacttgatgc aggacagctt tttcacgacc tctaattcac
     8041 ggtgttcaag caagaaatca ttgttcactg aaaagcgttg gatgttcttt ttcaaaggtg
     8101 tctaagatct ctcgtacttc atcagtagat ttcgtattca tcaactgaac tcgcaagtca
     8161 tttgctcctg gaaatccttt gatatagatt ttaaagaaac gatgtagtcc tgtgatggaa
     8221 cgcgggatga tttctgaata atgatcttgc agatctagtt gcagacgaag tagtccgatc
     8281 agttcttttt ctgtatgttc cctcggttct ttttcaaatg cataaggatt cttgaaaatc
     8341 ccccggccaa tcatgatgcc atctacgcca tatttttcgg ccaattcttc tcctgtctgg
     8401 cgatctggga tatctccgtt gatcgtgatc agtgtttgcg gagcgatttg gtcacgtaaa
     8461 gcaatgattt tagggatgac ttcccaatgt gcatctactt tactcatctc tttacgcgta
     8521 cgcaaatgga cagaaagatt ggctatatct tgatgaagta ggtgactgat ccattcttcc
     8581 atctcagaca tttccgtata gccgatacgc gtcttgacgc tgacaggcaa accgccagct
     8641 ttagccgctt caatcaattg tgccgctact tctgggcgaa ggatcaagcc actgcctttt
     8701 ccacgttctg ctacattagg gactgggcag cccatgttga tatcgattcc ttggaagccc
     8761 atttctgcca tagcaatgct catttctcta aaaaattctg gcttgtctcc ccaaatatgg
     8821 gcaaccatcg gctgttcatc ttctgtaaag gtcaatcttc ctcgtacact gtcttttcca
     8881 tctggatgac agaaactgtc tgagtttgta aactcagtaa agaagacatc cggtgctgct
     8941 gcatgcttta cgacatggcg aaatacaaca tctgttacat cttccattgg tgctaaaaca
     9001 aaaaaaggct ttggcagctc tttccaaaaa ttagtagtca tttatttgtt actctccatt
     9061 ctatcaagca gtgaagtcgc aatctttctc aaaaacattg tcattatact aggaaacaaa
     9121 aaaagaagca aagtgaacag cttaaaagat gtttctgatt gagcaaaaaa agactgtgac
     9181 aaaaatctta tcacaattcc ttttccggaa taaatggtgt tcaaaagctt gcatctgcgg
     9241 taataagtcc aaccaaacga aaaataggag gagctatttt tgtttggctg ttcttattac
     9301 ttgatgcaga acagcttttt cacaacctct ccactaaacg gtgttcaatc agctgaccct
     9361 cggtattagc tcaacaaacg acaaaatatg aggaactatt ttcccgtttg tttctctaat
     9421 actcaggtca aaacgctgat ttcacaacct cttttctaac ttctcaatcc tcttccttta
     9481 ttgatcaaat accaagtaac ggcgtacaaa acaacgatga atagcacaag tacgatcatc
     9541 gatgttgcta ctggcacatc gattgttcca aggaatccgt aacggaaacc gaagatcata
     9601 taaacgatcg gattgacttt cgaaacagct tgccaaaatg gcggcaacat agagatggca
     9661 tagaaaacgc cacctagata agttaacggc tgcaagacaa atgtaggcac gatcgataca
     9721 tcgtcataag actgagcaaa aatcccgttg agcagtcctg ctaacgaaaa caagattgcc
     9781 gtcatcaata atgtaatgat gaccatgaac catgaataca catgaagtgg tacaaagaac
     9841 aaggaaatga tcgttacgag agagccgacg agaatactgc gacccactcc gccaatcaca
     9901 aagccccaaa tgatcacatg cgtaggaaca ggcgctacca agatttcttc gatgtttttc
     9961 tggaattttt gagagaagaa ggacgaagaa acgttggcat acgaactagt gatagcagac
    10021 atcataatca aaccaggcac gatgaattcc atatatgaaa aaccacccat gtcaccgatc
    10081 cgaccaccga tcatttttcc gaaaataaca aagtacaagg atgtcgtgat cacaggcgga
    10141 accagtgttt ggacccagat acgcaaatag cggttcgttt ccttgatagc taaacttttt
    10201 aatgcagtaa aataaagatt aaacattttt ctcctccgta tgattatttt cttctgtgat
    10261 tttcaaaaac agttcttcta aacgattcga cttattcctc atagaaagaa ctttgattcc
    10321 ttgtcttgtc agctgatcga agattccgtt caccccttga tttcgttcaa cttcgacagc
    10381 gattgtctgt tcatcttcaa atgtacaagc gtatccttca atcacaggtt tggtctcata
    10441 tggcgctaaa tcaaagataa atgtttcaaa ttgcagtttg gatagaagct ctttcatact
    10501 agtattctcg atcagttcac ctgattggat gatcccgatg ttgcgacaaa gcatttcagc
    10561 ttcttctaaa taatgcgtcg tcaaaatgat cgttgtacca ttttcgttca attctcggag
    10621 aaactcccac atctcgcgtc tcaattcaat atcgactcct gctgttggtt catccaagat
    10681 cagcaaacgt ggttcatgca tcaatgccct agcaatcatc agtcttcgct tcattccccc
    10741 ggaaagcata cgtgcacgga catttctctt ttcccaaaga ttggactgct tcaaatattt
    10801 ttcgctgcgt ttcatcgctt ctttgcgaga gacaccgtag tagcctgctt gattcacgac
    10861 gatttgctgg accgtttcaa agggattgaa attgaactct tgcgggacta atccaatctg
    10921 ttgctttgct cggactagat ccgtatccag atcataaccg aagacattca cttttccaga
    10981 tgttttattg actaaagaag taatgattcc gatcgttgta gacttcccag ctccatttgg
    11041 tcctaatagc gcatagaaat ctccttcttt cacggaaaga tcgattcctc gcaatgcttc
    11101 gaccccactt ccataaacct ttttcaaatt atttatttca agcgcatttg ccatttattt
    11161 cctatctcct tatagacatg attctttatt gtatcatgga atacagcaga tgaaaaaaca
    11221 acttggttcc agcgaaaaat caggttgctg ggctcaggaa aatctcctaa tgaaagacat
    11281 caatcggacg aagtcagaca accttttcag aaaaaacaaa tcgaagataa aggattacta
    11341 ttcatatgtg caacaaaagg agcgtgagac aaaagtaaag aacacttttg tctcacgctc
    11401 taaacacgta taaacggcgg aagcaaaagc caagattttt gatccaataa atactttcga
    11461 tcacttcgcc tatcccaata catgccactg ccgcttttca tcaaataacg cggcttccgt
    11521 ttttgcggtt tctactaaag tctctgggta atccaaaact ttcagcaagg caatcgcatt
    11581 tcgggtcctt gaaggtcctt cttttaattt gaaatcaaaa ctgattcctt ttccttctgt
    11641 tacttgttct tcaaagtgga cattctcaca ataattcttt aaaatctctg tcaattcgat
    11701 atcatgtgtg gcgacaaatg ctaaagatgg atactccgca agccaccgaa cgactgaagc
    11761 ggaagaagca atccgttcga tcgtatttgt cccttttaaa atctcatcta cgaagcagta
    11821 gcagcgttct ttggaagcca cttggtccaa caaacgtttg atcgatttga tttctgaaac
    11881 aaaataactg tcgccttcaa ataaatcatc ttcgattgcc atggaagtca acacatgtcc
    11941 aggttgcagg gtaaactgtt ctgcaatcgc tgtttggatc gtctgtgcta gaatacagct
    12001 gatggctata cttttgacat acgtagattt tcctgatgca ttggaacccg tgactaatgt
    12061 attcttctgc cagttgaccg gattcactac cgcttctttt aacaatggat gatagatatc
    12121 tttcgctgtc acaccgccct cagtaaactc tggtcggcaa gtgatgggca tataagtacg
    12181 gtaattcaat attgcagcgg ctacttctaa ttctcccagc aattcccaca gcttgatggc
    12241 ttcagttgaa tgcttttcta atttagtgag aacgaaatga taggagataa atggcagcat
    12301 gaacatgatg ttgacatatt caaaaatcaa ctctgcttca ctcccactct tcatacgaaa
    12361 tgaaaaacct aatttaggga tgtctcttaa tggtttagtt tgctgtttca gctcttcttg
    12421 aagtggtgtc ggcagttttg ctagctgatt ggcggctgaa atcgttgaaa ccaaataact
    12481 catgctattc agctctgttt ctagtgtttg tttcttcact agataataga ccgtattgaa
    12541 tactaaccca ccgattgcca aatagattcc tagtacttgt cctaaaagaa gcaaacccaa
    12601 acccaccagc ggaagcaagc caagaaaaat aaataaacct acgcgtccaa tctttttact
    12661 ttttccatcc gctagatatt gttttgcaaa attatgatct ttttttccta aacgagcgaa
    12721 ttgatattgg attttttctc tcagttgagg attttcctga taaaaagcaa tcagtttttc
    12781 taactgctga tcttcaccaa aatcaaagct gcgtaaacgt tgatacaacg cttctgaacc
    12841 tacactagaa taggtactgt tgattccttc gaatacttcg aacatatcca ggtcatacca
    12901 agtaagatca tcgatttcgc tatcccatga acggaacttc ttctccgtga gccatgcttc
    12961 cttcaagctt tcttctttat caaatctcgg ttgataagga attttttccc atttgctacg
    13021 aactgtttcc tttaatttca aacgtgaaaa aatgtctaaa gcaactaata aaataacagc
    13081 tgcaataata attaaaacga taaattctct ttgacccata tccctctccg ttcctttatt
    13141 tgttagcttt agtatagcac tgttgcgcta tttgaaaaga gggtaaaaaa acacctttca
    13201 aaataaaagg tgtttttttc ttgtatatta catatttatc tctcatttat caaaattgac
    13261 aaataatcat agggaggaat tgttgttaat tcttgtttat tctccagtgc attgacaact
    13321 gtcccaaggg aaaggatagc ctcaggtact attttttctt gttctgttgg attagctaag
    13381 cgaagaacaa atccttgttc atgaaaataa gaaggataaa tcgatgatac aattaagtct
    13441 tcaggtaatt caatcaatga aaatttacga ggaacatcaa gttcattttg taatggccaa
    13501 atcttgttat ctaacctgtg aataaataca ttcagtttct gcttttgata agaaatagat
    13561 tgttctaacc gtttttctgc ctgttttgcc actttgaact cattaaatct tccttcttct
    13621 acattaaatc caaaagtgac tttatactca cctatctctt gagctagcgg tgtttccatc
    13681 atgacatgcc cttcatttgt cgtatctcct gaagctcgcc caggtcgcca agcaagatta
    13741 ggttttccaa gttcccctgt tgttgaaaat aaggttatga ttaattgatt ttctatacgt
    13801 tcatactctt ttaaaccatc tgcataaaac gtaaaataat gatcattttc tgccacagag
    13861 acactcttat cgaatatttc taaattaaca ggtttctcta ccatagtttc tgaccaattc
    13921 ggaggaatga catcaggggt attttcaata aatccgtttt gaatttgtgc aattgatttt
    13981 tcagatttat ctaaaacatt aattgctaag cgcatacgat gcgagtaaac agtattatga
    14041 attttgagtg tgccttcaac gatttgactt tctcccagac tgaacgatat ttcataagat
    14101 atttcttcca tttcgggttt ttcagataaa cgatctttta aatctttagg aagttgagct
    14161 tttccgtaaa caactaatgt ttcttgtaaa gaatctttat atactttagc tgtttctaat
    14221 tttagagata gttctgtatc tccttctaat ggtgaataat catacgtatc gccatcattt
    14281 gctgaatcga tcaaatgaac gaaatcaaga tattgtctat ctccaactat caaattcaat
    14341 tgcccatctt taaaagaaag tttatattta ttgttactta tctcagtttt gtctatctcg
    14401 attgctgttt ctaatttttc attatttttc tcaaattcaa caacagtgta tcctagtgct
    14461 ggcacattta catttatttt tatgtcaagt tcataataag ctggttcatc aaaatattcg
    14521 aaacctgtag caatcatccg ataagcattt ttccgttctg gaaaataaac ttctttttca
    14581 atgatattat agggactacc tttgaagtgt attttctttg aatcagctac tacatgcacg
    14641 attttcgatc cactataatt ctcagtcgtt gtgttgaaaa caaggacttc attcgatttt
    14701 agctttaatg catcagccat ccgttttaca ataaggtttt cgattccgtc agctatctca
    14761 ttagcttctt tgattcgatg gaaaatatct tccgcaacat tatctgaaac acagccacca
    14821 atactatcat gagcctggtt ttgcaaaatc tttttccata accgacgaag taggccttta
    14881 ctgatatgga taccatttct ttctgcaatc agcatgagcg gctcaactcg ttttagaatt
    14941 ttttgttcca atttaaaatt ttctattttc atttttgttc ttactgagcc aatagaacga
    15001 tgaacacgag agtaaaccgg ctgacgaagt tcacctttat attcaggtaa ttcttttttg
    15061 tttctgataa cttctacata agaaggataa tctgaaataa tattgtggaa tttactgtat
    15121 ttattcaact tctctacgac ttgatcaaaa tttaaaatca ttgacttttg atctacaccc
    15181 gaaggtacta aaatatcttt gttttttcct tgcatatgaa gcatttcaac tgcttcatcc
    15241 aacttttctt ttgcaaattc aggaatatct ttaattgcgt ctaaggtcat cattccagtc
    15301 atataaccaa atggaaaatt catggcataa acaaatttat ctcccaatcc tttccagata
    15361 aaataaggag atggaaccat cgtttcaaag tttaatcctc gccaacacat gaaattatcg
    15421 atttctactt gatgcaaaag tgtaggcaaa ttcgcattga acccaaacgt gtcaggcaaa
    15481 taccctacca tcattggatc tccatattta aggatcgtat cactgattcc aatcattaag
    15541 tttcttaaaa ttgattcagc atccactaat aaggcatctg tttgtgcata ccacggtccc
    15601 acgaatagtt gtcctgtttt catcagtcgt tggataacct taagcttttg cggattgatc
    15661 tccaaatact catcgacgat tgaagtttgt ccatctaaac aaaagtttac tttagggttc
    15721 ctttccaatt catctagaac ttcagtaaag acctgatcag acaatacgat ggcatcttca
    15781 tttgtaaaat accattctct gtcccagtgc gtgtgttgaa ttatatgtgc atttaccata
    15841 tcagattctc ctactttcct tcttctactg aatttacttg ttgttctttt ttatacttga
    15901 tggattttag tgcattgacc attaacgctg taaccactcc cccaataaca attgatacta
    15961 aatataaagg tatattcgta acaaaaggaa ttgccataaa ggttgaaagc attgtttctt
    16021 gagtaacacc aaaaaatcca tttagaccac atgctactgc agtgcccacc acgatgcttg
    16081 gtattactcg taaaggatct ttcacggcaa acgggatcgt cccttcagta atccccacaa
    16141 ggcccataat catagctgtc tttgcattat ctcgttccga ttcatcataa agttttttgt
    16201 ttaaaattgt tgctaaaaac attccaaatg gcggaatagc aattcctaat aacatggctg
    16261 tactaggtcc aaagcttccg cttgaaaaag aagcaacccc aaaagcatat gcgattttat
    16321 tgataggtcc tcccatatca aacgccatca ttcctcctaa aatagccgac atcaaagcag
    16381 catttgctcc tgacatgcct tctagccaat tagtcagtcc tgttgtcatt gcttgaatag
    16441 gcgtagctag tacataccat aatatgattc caacaactaa tgtagtaaca aaaaggaatc
    16501 agcataattg attttagact aataacttgc atcggtactt ttactttttt caatgcatta
    16561 gctaaatagc cacaagctaa tccagcaacc attcctccaa taaatcccat accaagattt
    16621 tgacacatca tcccagtgat aaaagctggt gctagaccgg gacggtcagc aattgaatat
    16681 gcgatatagg ctgaaatcat cggtacgata aactgtaatc ccgtctgacc aatcattcgc
    16741 aggactcccc agaatgtatc cgtctggtcc caaacacctg tgccaccaaa aatgactgca
    16801 atagaagtta gtaaccctgc tgctacgaca agcgggatca tataggaaac cccactttgg
    16861 aaatgattta gaatttctct tccttgtttc ttgaattcca ttagtaactc ccccacgata
    16921 tattttttat ttgttaatta atgcaattgc ttcatcaatc gtctcttcac catcttttac
    16981 tacctctgag gtccctactg ttaaaatcgg tttaccgtca aagcgacctc gttctgaaac
    17041 atttgtatct actgcaaata tgacaacatc tgcctcggaa acatccgaaa ttgaaagttt
    17101 gttttctatc cccatagccc cctgtgtttc tactttaata tctaaccctt tcttcttcgc
    17161 aaacttttct aaattagctt ttgccatata agtatgggca atccctgcga tacatgctgt
    17221 tactgctact atttttgtca tatgaaattc ctccttttaa aatgatagag cttttaccaa
    17281 ttctttttta tttctcgaat cttttattct tttaacaaac tcgtcatcca tcaatcttcc
    17341 agataattct gctaaaatac gtaaatgatt agtaccttta tcagtgtctt ttattgctaa
    17401 caaaaagacg agatcaactg gttgatcatc taatgcgccc cattcaacag accgctttaa
    17461 cttggctaca gccactgttg attcaattac tgctaggctt ttgccatgag gaatggcgat
    17521 atttttccca attcctgtgg tcatatggtt ttctctgtca aatacgtctt tttgaaattg
    17581 tttgtggtca gacacaaacc cattagtctc taacaatttc gataattttt cgactacact
    17641 ttttttatca gatgcattta aatctatcac tatattattt atattgattt tttcactgat
    17701 catttataag ccttctttca atatataaac taatttttca aacgacgaac tcgtttttaa
    17761 atttgctaat aactcatgat tcgaagcgat ttcattgaat gtttcataaa ttttattcaa
    17821 ttcaggtttt actttttcat taagtgcaaa aaagaaaaca gaatgaacaa gtgtcccatc
    17881 ccaatcaatt ccttctggat taacgtagac acagattact ggctttttta taaaatctac
    17941 ttcgatatgc ggcatagcaa aatcattcat cgctgttgaa gatagttttt ctcttttgat
    18001 tgctccctct agtatccctt ctttcgcgta attttctttt accaattttt tggtaattat
    18061 a
//
LOCUS       DAZUKC010000050        18008 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000050 DAZUKC010000000
VERSION     DAZUKC010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.051"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(24..722)
                     /locus_tag="ACG80T_001731"
     CDS             complement(24..722)
                     /locus_tag="ACG80T_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFS4841368.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="ACG80T_001732"
     CDS             complement(715..2067)
                     /locus_tag="ACG80T_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFS4841369.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="ACG80T_001733"
     CDS             complement(2290..3690)
                     /locus_tag="ACG80T_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFS4841370.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="ACG80T_001734"
     CDS             3851..4588
                     /locus_tag="ACG80T_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFS4841371.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="ACG80T_001735"
     CDS             4713..5192
                     /locus_tag="ACG80T_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFS4841372.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="ACG80T_001736"
     CDS             5189..5956
                     /locus_tag="ACG80T_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFS4841373.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="ACG80T_001737"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="ACG80T_001737"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS4841374.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="ACG80T_001738"
     CDS             complement(6859..7461)
                     /locus_tag="ACG80T_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFS4841375.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..7797
                     /locus_tag="ACG80T_001739"
     CDS             7567..7797
                     /locus_tag="ACG80T_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841376.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            7788..8036
                     /locus_tag="ACG80T_001740"
     CDS             7788..8036
                     /locus_tag="ACG80T_001740"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4841377.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            8142..8726
                     /locus_tag="ACG80T_001741"
     CDS             8142..8726
                     /locus_tag="ACG80T_001741"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFS4841378.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="ACG80T_001742"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="ACG80T_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFS4841379.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="ACG80T_001743"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="ACG80T_001743"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFS4841380.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="ACG80T_001744"
     CDS             10627..10806
                     /locus_tag="ACG80T_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841381.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="ACG80T_001745"
     CDS             10828..11853
                     /locus_tag="ACG80T_001745"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFS4841382.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12028..13272
                     /gene="glyA"
                     /locus_tag="ACG80T_001746"
     CDS             12028..13272
                     /gene="glyA"
                     /locus_tag="ACG80T_001746"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFS4841383.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13425..14372
                     /locus_tag="ACG80T_001747"
     CDS             13425..14372
                     /locus_tag="ACG80T_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFS4841384.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14506..15135
                     /gene="upp"
                     /locus_tag="ACG80T_001748"
     CDS             14506..15135
                     /gene="upp"
                     /locus_tag="ACG80T_001748"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFS4841385.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15274..15516)
                     /locus_tag="ACG80T_001749"
     CDS             complement(15274..15516)
                     /locus_tag="ACG80T_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841386.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15637..15709
                     /locus_tag="ACG80T_001750"
     tRNA            15637..15709
                     /locus_tag="ACG80T_001750"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15670..15672,aa:Thr,seq:cgt)
     gene            complement(15828..16466)
                     /locus_tag="ACG80T_001751"
     CDS             complement(15828..16466)
                     /locus_tag="ACG80T_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFS4841387.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16589..17221)
                     /locus_tag="ACG80T_001752"
     CDS             complement(16589..17221)
                     /locus_tag="ACG80T_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFS4841388.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17572..17871
                     /locus_tag="ACG80T_001753"
     CDS             17572..17871
                     /locus_tag="ACG80T_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFS4841389.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT         6166 a         3154 c         3557 g         5131 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
    12061 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
    12121 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
    12181 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
    12241 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
    12301 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
    12361 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
    12421 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
    12481 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
    12541 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
    12601 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
    12661 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
    12721 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
    12781 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
    12841 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
    12901 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
    12961 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
    13021 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
    13081 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
    13141 gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat
    13201 aacgctcaac tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg
    13261 cataaaaaat aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc
    13321 tgtttatttt ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga
    13381 ggtgtaattt tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct
    13441 cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg
    13501 aactagcacc ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca
    13561 aatattccgt cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata
    13621 ttcgtctcgt acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg
    13681 gagcaggctg ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg
    13741 ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc
    13801 caacagcaat tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa
    13861 aaaatctgga tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta
    13921 cagtcatgac gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt
    13981 attatccagt cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact
    14041 attttttgac aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag
    14101 agcgtgctga aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc
    14161 cttcagcaat gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg
    14221 cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc
    14281 atgattttgt catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat
    14341 tatctacttc ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt
    14401 ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg
    14461 ttttacaatt aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa
    14521 gtgatcgatc atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga
    14581 acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca
    14641 cgggatatgc cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa
    14701 acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt
    14761 gacggtatct tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat
    14821 gaagaaacat tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt
    14881 cagttgtttg tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat
    14941 gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag
    15001 ggagtaaaag ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat
    15061 gaaaaattga atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg
    15121 tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt
    15181 ttggtcattt ttaacgtttg atgcagaaca gctttttcac agctttcgtt tgcatataaa
    15241 aaaaccatcc caaaaatgca gagatggtca aattcaaatc ttttttagtt ctttgtagtt
    15301 aacgattact acacccttac tttgcgtcat caagttcgca ttttctgcag tttcatcaat
    15361 ctcaacaaca gcggaatttt tcaattgctt tgaaatcgtt ccagtttgcg acttgccgtg
    15421 aattgtgaac attacgtgag tattgatctt gaatttttca ccaatttccg atggtacgat
    15481 agttgtatca aattttccaa aagttgccat tttcataaaa cctgcctttc acatcaatta
    15541 taccacgttt tatgaaaaat atcatgaaat taaactaatt ttccatcgga ctctttacaa
    15601 tcagccaaat agagtatata atttcttttg gataaggcca gcttagctca gttggtagag
    15661 caacgcactc gtaacgcgta ggtcgtaggt tcgatcccta tagctggcat taaaagagaa
    15721 gacagatata tcaaggattt ttaatccatt gatacatctg tctttttgtt taaaggaaat
    15781 aaagtatgct ttcttagcaa tagagcatgt attatttcta taaaaattta ggagaataaa
    15841 acgactagca atatagctgt gaaatcaaat atcacatgaa ctgcccaact tgtaatgatc
    15901 gagttgcttt taatggcagc ttggttgaat aacaatcttg ccaccccttg tatcaaaagg
    15961 atgtgtgcta cagtgtgtaa aatatttcca ttatcataag tactgtaatg gataaatcca
    16021 aagatgattg ctgacaagaa actagcgaca ggaatgctga acttactaga aaaaatagcg
    16081 agaaaataca ctgagaacaa ttcttctcca agtatcatga taggcaaaat aataaataat
    16141 aacgggctga aagcctcatt tattggattg cctgttaaac cccatttcaa aatttgttgc
    16201 aaaaataccg agactgcggc actgattact acattgataa aaaaatattt ggctatattt
    16261 ttgacaggag cgacaggttt cgagaacatt tttttcgtat cttttttacc aaaaacaagt
    16321 gtaaatgcta taaatccagt gatagttaaa gaaaaaatgc taataatgga tggcgcaaat
    16381 agcaacccta acaaaccgag tacaacgaca atagtgccca atatcccttt tttaagttct
    16441 attttttttc ctaaaatgtt attcatgata ataactccta ctattcagtt tttgaagata
    16501 gagtcagtat acatgaagag ttaaaatagg caatgttttt ttatggatat ttgtattgct
    16561 aaaaatttat cttatatatt cattttgatt atactacttt tatttgtaaa aattttctag
    16621 caaaaccaag tgcttgcaac tgattaagcg tacaattttt tagatgttcc gagcgtactt
    16681 caatcatttc aaaggagttg ctacttaaat cgatattttt taaatcagta tcaaaaaagc
    16741 tagcattttc taaatcatta gtagaaaaca ttattttagt gagggaacag tctacaaaat
    16801 ctgcgcttga taagacagag tgctcaaagg tactttcttt tatgtttgaa ccagataaat
    16861 ttacatagtc cattttacaa ttctcgaata acgtgttccc aatataactt tccatcaatt
    16921 tagtacctgt taatttacaa ttgacaaatt tcacgcgatg caacatgctt tttgtcaata
    16981 tacagcctga aaaatcacag ttttcgaata tacagtcggt aatctccatt ctttgaaaat
    17041 cattggaagc aaaaaatgca tacttgaaaa tagatttgta aataattgga tggcagatta
    17101 tttggttttc gatgacctct ttttgtgaaa ggatattata cagttcaaaa tcttcgtttt
    17161 catagatatg aaaaaaggat cctttattca agcttgcttc attaacttta ggaaaaatca
    17221 tatttaatta cctccatttt cataaagtca tattaacaaa agctaaacca acttacatta
    17281 gcagaaaatg agataggttt tcgccttttt cgataacgat agtgtcgcta tctcttgaaa
    17341 cgttcttaga attgccctga gagacacaaa ttaggcgtaa ttctaataaa aatcttagaa
    17401 taaaaatttt tactgattta atttagaatg tttttaaaat aaaaatcgtt gccataacag
    17461 tgtattgaaa ggttttaaca atccagtaat gatttaaaaa aatctagatg tagcgtatac
    17521 tttgtttata gaatgtagtg ggaagaaata tatttttggg aggaaatcgt gatgtctaaa
    17581 agtgaagaaa tagaaaacat ggtactaaaa ttatatgatc tcgttttaaa tccagagatc
    17641 aaagaaaagg aacgtatact tttgatagac gcaaagacag gcttagaaaa aggtcagtat
    17701 tatcctaaag tcataaataa tttggaacgt agtttacgtc cgttagcaat caggggagaa
    17761 ttgtctaaac cagtatcacc gttttatatg gagatatcaa caataggaaa atttgaaaaa
    17821 gaattaggtc gaggaatggc ttcggcaccg attacttttg ggcatttata aaaacgagtt
    17881 attcatctca atgaattaaa aagtatagtc aatctcataa tgcaggctag gttcatttat
    17941 ttgaacagag tctgtatttt tttattctaa ataaagaaac agatgtttct ttatattgta
    18001 ttttatcc
//
LOCUS       DAZUKC010000051        17298 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000051 DAZUKC010000000
VERSION     DAZUKC010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.052"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..535)
                     /locus_tag="ACG80T_001754"
     CDS             complement(<1..535)
                     /locus_tag="ACG80T_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFS4841390.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGA"
     gene            complement(547..1239)
                     /locus_tag="ACG80T_001755"
     CDS             complement(547..1239)
                     /locus_tag="ACG80T_001755"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFS4841391.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1258..1542)
                     /gene="macP"
                     /locus_tag="ACG80T_001756"
     CDS             complement(1258..1542)
                     /gene="macP"
                     /locus_tag="ACG80T_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFS4841392.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1555..2118)
                     /locus_tag="ACG80T_001757"
     CDS             complement(1555..2118)
                     /locus_tag="ACG80T_001757"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFS4841393.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2346..3008
                     /locus_tag="ACG80T_001758"
     CDS             2346..3008
                     /locus_tag="ACG80T_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFS4841394.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3132..4328
                     /locus_tag="ACG80T_001759"
     CDS             3132..4328
                     /locus_tag="ACG80T_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFS4841395.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4429..5805)
                     /locus_tag="ACG80T_001760"
     CDS             complement(4429..5805)
                     /locus_tag="ACG80T_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4841396.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5842..6708)
                     /locus_tag="ACG80T_001761"
     CDS             complement(5842..6708)
                     /locus_tag="ACG80T_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFS4841397.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6797..7822)
                     /locus_tag="ACG80T_001762"
     CDS             complement(6797..7822)
                     /locus_tag="ACG80T_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4841398.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7840..8514)
                     /locus_tag="ACG80T_001763"
     CDS             complement(7840..8514)
                     /locus_tag="ACG80T_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4841399.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8698..9591)
                     /locus_tag="ACG80T_001764"
     CDS             complement(8698..9591)
                     /locus_tag="ACG80T_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS4841400.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9665..11362)
                     /locus_tag="ACG80T_001765"
     CDS             complement(9665..11362)
                     /locus_tag="ACG80T_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS4841401.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11355..12668)
                     /locus_tag="ACG80T_001766"
     CDS             complement(11355..12668)
                     /locus_tag="ACG80T_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFS4841402.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12668..16609)
                     /locus_tag="ACG80T_001767"
     CDS             complement(12668..16609)
                     /locus_tag="ACG80T_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS4841403.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16718..17176)
                     /locus_tag="ACG80T_001768"
     CDS             complement(16718..17176)
                     /locus_tag="ACG80T_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4841404.1"
                     /translation="MKGLKLKILQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         4851 a         3892 c         3010 g         5545 t
ORIGIN      
        1 ctgcgcctag tgtggactca aaatgtctca atgcccattc atgtccaacg ggatcgaaac
       61 ttgccacgga atctttgaaa atatggagtt tgtatcctag attatacgca tcgactgctg
      121 tatgcaacac acagatatct gtacatacgc cggtcaaata aatatctgtg atctggcgtt
      181 ctcgcaaacg gatgtctaga tctgttccac taaaagcgga ataatgccgt ttgtctatcc
      241 aataaacatt ttcctcttgc tcatagtctt ggtataaact ttttaaagag ccgtacaatt
      301 cccgtccacc cgttcctatc acattatgcg gaggaaataa gcggttctcc ggatgaaagg
      361 aatcttttgg atcatgcgca tcaatggcaa aaacgacaaa atccttttga tcaataaact
      421 tctttgtatg agcagtcagc tttttttcaa ttacttgtcc tgctgtccca gtagtcagct
      481 ttccatcctc tgcaacaaaa tcatatgtgt agtcaatcga gatcaatgcg ttcatcttag
      541 tcctccttat acgagatgtt tgacaaattc gatcaccatt tctgctgatc gtttgcctgc
      601 gtcttcgata aattcgtcaa agctttgtgt cgccgagtga tctgctgtgt cgctcattgc
      661 acgtacgatc aaaaatggga tattgaactg acgagctgtt tgtccaacag cggctccttc
      721 catttcgcaa gctaatgctt cagggaaatt cgttaagatt tctttgattt tatctggcga
      781 gtcgacaaat gtatcacctg taacgatcaa tccttctttt gcgttcaaat tcgttgcttt
      841 tgctgcttta accatttcac tgcgcaaata tgtgcttgct tcataatata gaggcattcc
      901 tggtaattgt cctgggttgt agccaaatcc tgttgcatct gcatcaaaat aagctacttt
      961 gtctgatata acgatgtcac ctacttgtaa gccttcaccg attccgccag ctgaacctgt
     1021 attgatcacc atgttcacgc cgtattgttg gatcaacaga cttgtggtaa tagatgctaa
     1081 gacttttcct ataccggaac gtacgacgat cacttcgtgg ttacctaatg aacctgaaat
     1141 aaataaagcg cctgcgcgtt cccatgataa tggttcgctt aggttctctc tcaaaatttt
     1201 tacttcttct tccattgcgc cgataatacc gattttcatc tttttaattc cttctttcta
     1261 tataaatgcg attgctaaaa aaaccacaca taacattagg ataacaataa taagagattt
     1321 catcaaaaaa gagttccatt tggtcgtctt ttcccgttcg ctgatccttg ttttcgtgat
     1381 tggtttattc tttttgcttt ctttacgata aaagctggca atcttttttt cttcttgctg
     1441 ataagctgtt tctgcttttc gctgtttttt taatgactcg cttgcttgtg cttgttgtcg
     1501 cttgcgaagt tcgcttcgcg taataagcgg tcctttggcc ataagccgat ccctctactt
     1561 tcctttggtc aatagttccc agtattgaat cgcataaatc gtctttgcat cacaaatcaa
     1621 ctgatctttc atcgcttgtt gcgcctcttc aagagttaga tgatacaact ccagtacttc
     1681 gtcttcatct tgtgctaaag gattctcgac cttctctacc ccttgtgcat gataaatatg
     1741 aaggacttca tttgcaaaac ctggtgaaag atacatcgaa gtaagatggc tcagcgactt
     1801 cgcacgatag ccagtttctt cttctaattc tctagctgct gtcatttcgg gattttgtcc
     1861 ttctcctgga tcgatttttc cagctggaat ctccaaaatc actttttcta aaggttttcg
     1921 aaattgtttg acaagaagca gacgatcttg ttcgtcaaaa gcaatgatcc cgacaccgcc
     1981 aggatgaaat accagttctc tcttcgaagt tcctccatct ggcaaacgaa cttcgtctac
     2041 agcaacatca ataatttttc cgctatagat ttctttgcga ctgattgttt tttcttcaaa
     2101 ttgctcattg ttcagcatat cttctcctgc tttctttgta atcataactg cttttttttc
     2161 atttttcaat ttttctcaaa aactccttat tagtttacca tagataaacc ttagattttc
     2221 tctaaatata gaaaatcctt ctttagtccg atactctttc tagtagtccg tatgctaaaa
     2281 taagtatgta atcgaataac gaaaagaatt caatactcaa cgataaatga catggagggt
     2341 ttattatgat aaaacgaaat tggccgtggc ttctagcatt ggtcttgcta tttctctttg
     2401 tccgaaacaa tagtttttct tttgttctag gacttttatt gataggtgta gttttagttt
     2461 tgtggggact ctttggttcg cgcaggaaaa agggaaaaca agaagaaatt cctgcactga
     2521 ctgacgaact tgaaactcac tatgaaaaaa gcggtatgac agcgaatgaa atttcatttt
     2581 ttcgacaaac aatgaatcaa acaaaaaatg agatcaatca attgcaaatg aatatgcagc
     2641 agacagccaa acttaaagca atcgatctcc gccatgatcc agtcaaagca gcgaaagctt
     2701 tattcaaaga attggtgaaa gaaccaaacc gactgcatga agcaagtcag tttctttata
     2761 cgcatctgcc gaatctagtt gatttgacaa ataagtataa tgagatcaat gaccatgaga
     2821 tcaaaaacaa acagacctat gaaaaactag aagaaagcgc gcagatcatc gaccagctgt
     2881 cagctttaat cgcacaagat tatcaaaaat tcgttgctga ggatctagat gatatcgacg
     2941 tagagatatc tgttgcgaaa cagagcttaa agcgtgataa taaatatgaa ccagaacaaa
     3001 aggattaact cttttgttca taaaagaaga ctcggaacat tcagatgatg tctggctgtt
     3061 tcgagtactt tttctaaagt agagacattc atgtaagaat cgagaacaga ttcaacaagg
     3121 aggaattttt tatggatcat aaaccgactg aaacaaaaga tgttacacca gttaacgata
     3181 cacttgaaga tttattgaac aacccgtttt caactccaac agatacgctg accacttcac
     3241 aacaagcgga gattgatcag ctccaagagc aacagacagc tgctcgttta attgacaaac
     3301 taccggcgga acgccaagaa caagctaaac agctagcagc aaaaatcgat gctaatgatg
     3361 cacagtctgt catcagctac ggatcggctg cccaagcaaa attaagtgaa ttctcacaat
     3421 cgatgttgaa tcatgtgcaa gcacaagata ttggtcctgt aggagattcc ttaactgaac
     3481 tgatgtatcg tctccaagaa gccaatcctg acgaactgcg tgccggagaa gggaacatct
     3541 tccagcgtgt cttcgggaag gtcaaacaat cgatctatga agtgacagca aaatatcaaa
     3601 agattggtgc acaaattgac aagatttcag tgaaattaga taaagaaaaa gacggtctgc
     3661 taaaagataa tctgatgtta gaacagcttt accagaaaaa caaagactac tttgatgcac
     3721 tgaatattta tatcgctgct ggagaattga aaatggaaga acttcaaaca acgatcattc
     3781 cagaagcgat gaaaagagca gaagaaactg gcgatcagat ggatgttcag attgccaatg
     3841 attacacgca attcctagat cgtctggata aacgtacgca tgatcttcgc ttagctagac
     3901 agatcacgat ccagcaagca ccacaaatcc gtttgatcca aaacacaaat caggccttgg
     3961 ctgaaaagat ccaagcatct atcgctacag ccattccgct gtggaaaaac caagtcgtga
     4021 tcgcattgac cttgcttcgt caaaaagatg cagtaactgc tcaacgccaa gtctctgaaa
     4081 caacaaacga cttattgaag aaaaattcag aaatgctgaa aatctcagcc atcgaaacag
     4141 cgaaagaaaa tgaacgtgga atcgtggata tcgagaccct tcaaaccact caaaatgatt
     4201 tgatcgaaac aatccaagag acattgcgta tccaaaaaga aggaaaagaa aaacgtcgtc
     4261 atgcagaagt agagcttgga cacatggaag aagatctgaa acaaaaactg ttggatttaa
     4321 cccaatagat cacctttcgc ttgttactaa aacaaacgcc caacaacagt ttattacaac
     4381 tgttgatggg cgtttttctt atctttcttc taacttctcc attggagtct atttctcatt
     4441 catcttgctg tgtccatagc catagccaaa ataaatgatc agtccaataa ccattacgat
     4501 accgaacacg atccatgtca cagtttgcag acgaagcatc aatacaatac aagtcaagat
     4561 ggagacgatt ggcaatagcg gaacgaatgg cactttgaat tctccagctt ttggtttgcc
     4621 aaacattttt ctcaacttca gtaagcctag actaacaagc atcaaatagg cgagcgccat
     4681 gatattcgtt agttccgcta atacattcaa tgggaaaaat ccggcaaaga atacagctgc
     4741 cacaccagcg gcataagtcg cttttttcgg tgtacgatgc ttttgatcga tttctgtcat
     4801 aaacttagga agtaagccat ccttgctgac agcaaacagt aaacgagcaa gtgaatacat
     4861 catcgagatc gttaccgtca gtagcgttaa aattgcccct acagcgatca ccgcgcccac
     4921 tgttccatgt cctacatacc tcattgcaaa agccactggg tcattgactc ctagttcagt
     4981 atacggaacg attcctgtca agatcagcgt cacgatgatg taaaggacag tagcaatcag
     5041 tatcgaacca atgattcctc ttggcacatc tttttgcggg tttttgactt cctcggctgc
     5101 cattgcaacg gcatcaaatc ccaagaaagc aaaaaacaca agagctgcac catctagcac
     5161 accggaaaaa ccaaaaggca tgaatggctg ccagttatca ggtttaacga aaaatattcc
     5221 tacaatgata aacaacgcga tgataccaaa tttgacatat accatggcat tattcaaacg
     5281 taatgctttc ttggcttcaa gcgacacgat gtaagtgacg aatatcacta cgagcaccgc
     5341 aatcaagtca atgtatgtac cattttccgg attgtacccg ccagttaatg cttgtggaag
     5401 ttcggcccct aaacttttca aaaaaccttg catatagcca gaccatccgg aagcaacaga
     5461 ggaaaccgct agtaaaaatt ccccgatcaa caaccaccct gtcagccagg cagcaaattc
     5521 accaaataca acatacagat aggcatatgg cccaccaatc actggcactc gcgaagcaaa
     5581 ttctgcgaaa cttagtccag acaaaatgac aacgattgca gctaagacaa atgaaagtga
     5641 cagtgctgga ccagcatttt gattggccgc aacccctgtc accacaaata ttcccgtacc
     5701 gatgatcgca ccaattccaa gcatgatcaa atcagacgtt tttagttctt ttttcatctc
     5761 actcgtatga ttgagattga tctctttctt acgaaacaaa tccattgaag ttcctcccca
     5821 ttttaggttg gaaaacagcg attaaactgt tttcatatac cctaaaagga taccacctaa
     5881 aatgactaaa aggcaaccaa caataacata aatgagctct tttttcgttt ttctttcgcc
     5941 aaggatgaaa atcccaccta aagtagagat aatgatcccc atttgtgaaa gtgagaagcc
     6001 aacagctaaa ccaagtgatt tgactgttag aagcatgcag atgtttccta agccccataa
     6061 taatccacag atcatgtttt tccacacaaa ctgatcgact ttaactttac ggaaagcaaa
     6121 gaagcttgcg cctaagatca ttccgatact ttgaggtaag ataattgaca tcgcatccaa
     6181 attcgcccag ttgacgatga tggtatatac accataacca atcgtagaga agatcaatgc
     6241 tctaaaaccc ttaccaaaat caagcatttt attatctgta tctatttttc cttcgtcatc
     6301 ttgtcgggca gtcaaatacg caccgcttac aagaagagct aaagcaataa taccgtatac
     6361 ataatctttc gtttgtgtcc attcatggaa gaaaacggct cccgcaatcg tattcaaaat
     6421 taattgaaaa ccagtagaca atggtagccc tacagaaaca cccatgtact tcattccatg
     6481 gaactgtcca ttttgtccaa cactccaagc taagccggat aaaaatccga tcaagaaaat
     6541 ccaaggggtg attacaggac taacaacgaa aaagacaact aaagaaaaca gaaatgcacc
     6601 gatcgtcatt cctagtgttt gttgattggc atcgccacca attttaccac ttactaaacc
     6661 aatactaccc catgccaaca tagggactaa tgctattaaa atttccataa ccaatctcct
     6721 tttatttcac atgataagaa acttatcagc gaaaacgtta acaacacaag cacttttttt
     6781 cgtttctaac gattttctaa cgtttttcat tataaaaatt taagattggg aaaaataaag
     6841 tcattgttgt cccttttcct tcagttgatt cagccgtcaa atggatacct aatttttcgg
     6901 ccagattttt tgctaaataa agtcctaatc ctgttgaatg tgttttgcta agtcgaccat
     6961 ttctcccagt aaatccctta tcaaagatcc gtttcaagtc ttccgctggg ataccgatcc
     7021 ctgaatcttt aactgaaagg ctgactcctt gcgcttcctt ttctatgctc acaatgatgt
     7081 ttccgtgatc cggcgtatat ttgatggcat tactaagcaa ttggttgaag ataaaagcga
     7141 tccatttagc atcggtcaaa acagtatgat cctcaccaac gatctcgtag cgaatatttt
     7201 tttggataaa ataatttgcc tgactgcgta tcacaggctg aatgattcct tttagtccat
     7261 attcttggat gaggtagtcc cgcgagaaac tgtcgagtct tgcataatac aaaacttgtt
     7321 ctacgtattc atcgattttt gataattcat tttctagtaa tgtatacttt tgatcgtcaa
     7381 tatcgaactc gatcgaacga actagcaatt tagaagcgga aagtggcact ttgatttcat
     7441 ggacccatga gtcaatataa tctttttggt cttgttgatt actgattgct gattgcatta
     7501 cttcttggtg ttcacggatc agctgattga tatattcttc taccagtttt tcttcttctg
     7561 tcctcccacc actcaaataa ttttgcaaag gggaagtttt ttgtatgtcg gctaattttc
     7621 cccaccatct tctcttgctc aagtaaccac ccagcagata aaaaatcaga aagacgccct
     7681 caatcaaagc taaataagca attgtgctca aatcaacttt gatgtttggc gccagccaca
     7741 tcacaaagat cgtcaatgca ataaaaaaca gccatcccaa gatcaatgtc cactgatctt
     7801 tcaaatattt aaaaaaattc atcttaaccg ctcatccttt cagggaacga tatatccctg
     7861 tcctactttt gtttcgatat atccttggat acctgcctgt tcgatttttc ggcgtaaccg
     7921 attgatgttg actgtcaatg tattgtcatc tacaaatcgt tcgtcatccc acagtgctcg
     7981 cagcaatttt tcccgactta agatctttcc attgtgcttc atcaaaataa ataatagacg
     8041 atattcattt ttgctcaaat caatgatctc tccgttgatt tccatacttc cgttgtcaac
     8101 attcaacgta atgccattgt ggctcatcat ttcactagat aattctgtgt agttgtaaga
     8161 acggcgaagt aatgcattga tttttgcaat cagtacatcg atttggaaat gttttgtcac
     8221 aaaatcatct gcccccatgt tcatagccat gatcatatcc atattcgtat tcctgctgga
     8281 gataaaaatg atcggtactt tcgatacttc acgtattttt tgacaccaat aatagccatc
     8341 caaaacggga agattaatat ctagtaatac aagctgtggt tcttctcttt caaagtctga
     8401 gaacacttga ttgaaatcgg tcgttccaaa ggtttcaaat tgccatttct caagttcctc
     8461 catgatcaat tgtcgaatga tctcttcgtc ttcgactacc atgatttttg ccataaaaaa
     8521 tccctccatc tttcttcttg ctactacaat atcaaagaag cccttgtgaa acaagttggc
     8581 ttacctggaa aatgtcattt tagcaaacgt taaaaaaaac tggaaatatg cgatttaatc
     8641 gcgtttgtct cttccaagca gcttaaattt attatgttat tcttaataat tggtctatta
     8701 tctgttattt ttctttcgtt gtttttgtct tttaaatttt cgataacacc aataaccgaa
     8761 aagcgttagc actagcaata tacctagaag gagtataagt aatcgataaa gatggtattt
     8821 ttttgtctgt tctatctttg aagagacttc ttctgtaaag ggaactcgtc ttcctgtcac
     8881 tagtaatcgg tgagtgttca cggtgtaagg tgtacaagtg ataagtgtga ctagatcctg
     8941 cttttctcta attgctaatg tatttgtttt tgttggaagg acaacaattt tctcaacgac
     9001 ttcataagcg agcatccgac catacacttc aagatagaat cggtcatctt gctccatttc
     9061 ttcaagatcc gtaaagagtt ttttctcagc aataccgcta tgggcggtaa tcacggaatg
     9121 tgtactctct cctccagttg gaaaagaggt gccttgcagg actgtcgctc ctctatctaa
     9181 caaaagatca ttcgtttcat caaataaagg caagctaaca ttgattttcg gaatatagat
     9241 ggctccgatc atatgttgtt cgtaatattc ttttcctgga ttttggacat ttctaaccgc
     9301 ttgctcaaaa ggatctttga cttgtcccat tcctgggatt actgatgtat gagcaagcgt
     9361 ctgatttttc ttttcatgca ctgctaattg ttgctcatgt ttttcttctt tatcgcgttc
     9421 aagctgcttt tgataattct cgatccttct ttgatccaaa taattgctga gagcatctgc
     9481 taaaaatgga tatgtaaaaa ctgcagctcc ggcaagaaac atcactgcca taaataattg
     9541 taataaaaga ctgatttggg tttgaggtct attacctatt ttctttttca ttttcttcac
     9601 cttcttattt aaaaaaactg cagcaacctt tataaaaccg attgctgcag cttatttttg
     9661 ttgcttatac ttcagacttc attttagatt tacggtacca gctgtacgct ccaatcatca
     9721 aacttattcc gattagtaag aatgccaaga gcccgttccc accagtagca ggaagtgacc
     9781 cgcgtttcat attcggtact ctttctggcc ctggattttc tactcctacg atctgaattt
     9841 cttcattata gcttccataa gagatgataa attcagtaaa ggctgcctca cctgttggaa
     9901 gtacataacc ttctggcgcc atggtctctt tcatttgata ggttccataa tctaaccctt
     9961 gaacctcaaa gtatccattt ccatctgcga caaattctgt tgcttgtccg cttgttgccc
    10021 actcaattct atctgctcct gattcataaa cagctaaagg cgactcccct ttataaaata
    10081 ttgcgtattc agaacggtct tcattccaca attcgaatct tgcatcttta agcaattcac
    10141 tgcttgatgc atcatatttt tcaaactttc ttccacctgt tgtgactttc tctggcggct
    10201 ctactccttc ttcataatca aattcatcat ttcgtccgat ttcaataacc gctgtgttgt
    10261 tatggaaatc atccgcttct agttctgtgg aaaccttcaa attatacgtg atcgttaaat
    10321 ctcctccacg tgcatctgct aatctaccta aagtatctac tttggctact ccattttctt
    10381 ctgtcaaaat cgtcacgaca aacccgttgc ttactacttc tactatataa tcttgtcctt
    10441 ttgttaaaat cgtctcgccg gctcgcactt ctatttcctg atcataataa gttagaccaa
    10501 ctgtaggcgt atctgtgatt ttgtaatttt tgataacagc gcttccgttt tcaaggacag
    10561 aatcgatgaa ataaggaata ggtgctgtga tcgtgtaaga agccgttttt cctctttcta
    10621 gatcaatata agaaatcgtt tcttgttctc cagcttcatt aataatcgtt acttgtctaa
    10681 ggtcgctttc ctctacattc atctgtttat ctgcttctct gatttcgttt ttcggataca
    10741 aatgcacatc tgtattgtat gtgtctttcc atgtagaacc atcccacacg ccatccacca
    10801 tatcaggcat catcactggc atcataacaa ccattggaga cgcgattctg gaaatatgag
    10861 ctggcgaata agtttctaag aaaagataga ctttatctct agaagaaggt gcttcttccg
    10921 tcacttggac ggtagagaat accgcttctc cagcttcatt cgttgtttgt ttgtcaatac
    10981 ttgtcagtcg atattttaaa aaccaacttt ctgtgtgccg tttttgaatc cattcaatcg
    11041 cttcggcaga attaagaccg tcttctcgtt ccggtgttaa aggattatct cgaatcaaat
    11101 cgtaatatac ttccgttacg tcaaacaaat caaaatcgac tcctggaata ttttcttcac
    11161 caaaatccgt agaaaccaat ccgctattcg gtcgttcttc cggaacagat gagaaacctt
    11221 ttttgtgtaa tgtaatcgtc acttcacttg gtttttgtgc gacttcttcg gctttgacta
    11281 gttttccgtt cccgaaaatc cctattcccg cagtaacgag taacagtaca ctcattcccg
    11341 tgacaatcat ttttttattc atttttttct ccccgttctt tttttattaa taaaacagca
    11401 cctaacaata aaagaattcc tagtaaagaa atcaacgttc ttgcttcatt cgtttgcggg
    11461 aatacggacc attttcctga ttgacctgga ttgcttggac cactcggctc tttcggtttg
    11521 tccgttgttt cttttacggt ctcgttataa acacgtggtg ttgcggattc ataggcagag
    11581 actggaactt tcccggattc taattcagcc atctcctgtc cgttaactac tacatattgt
    11641 ggttggcctt ttgcatccac ccaaaattca gggatgatga cctcgattct cttacttgct
    11701 tcatccatct cgtaaccttc cacacctttt aattcttcaa aataataggt gccagaaggc
    11761 aagaaacgtt gtcctgtcgt aacaaggccc tctttatccg aaatgaattt gcttacattt
    11821 ttgtcattca aaggatcatt gtctgctggt ttgatccatt gatttttcaa gtcattagca
    11881 ggagccatat ctaaataata tttcccccct tctatcatct gatatagagc aaaaacagca
    11941 ccttctaatg gttttccttt ttcagttgtt cctctttgac gtccatactt gaagaagtat
    12001 ggatctctta gataaccgac atttttagga tagatatgaa tctcttgcag ttcctcggtt
    12061 gattctgtcg ggtgataaat tggcaaaata gcagcaattg ggatagctgt ctgatcgata
    12121 tcaacatcca atccaacttc tggatcaatc gattcttcga aaatcaaata aactgcatcc
    12181 cgatcttggt ttttcttagg tgctttgata cgtgcaatcc cgtctcctaa tccgccaacc
    12241 gtatctttcc cagttgttac cgaagtccct atttggacaa gcctttcttc tgtgattagc
    12301 tgacggatat tgtttctgtt cgtttcagcg atttgctgga cgaattcttt aggagtcatg
    12361 ttttcagcag cttttttttc atgataataa tcggtcatgt cgtaaatggc aaatgtcgct
    12421 ccatttagtg gcaccgtctg tgaaatgata tctgctgttt cccctgcttc ctcagagata
    12481 gccaagccat cattttcatg gtattcaaat tgatcgttat aatcgatatc tcgaatcatc
    12541 cgtttatgca acacgaattc cacgacatct tcccctgatt cttctgccga catcgcatgt
    12601 ggaagaagga ctgctgcaag cggtagtaaa acaagtaatt gaagcatcaa acacaacagc
    12661 tttttcatta ccttgctccc tcctttttag attgtctgac aaacaaacca taggctgctc
    12721 ctgcaagcgc catgaagaat gcagcaagca ggatatagaa taatcgtccg gatcctcctg
    12781 tagcaggcaa cggattttca ggatcgttcc cgacactaag ctgtaatctg attcgatttg
    12841 tctccaaaag ttcatattct aactcttgct gtagatcagg cgtctctgct tcttcgtttg
    12901 gataatacga aatctcaact tttagtgtac ccgcttcttt tcctggaact acccgatcat
    12961 tttctatgtc aggttcttct cgataactaa tttctaaaat aaagtaacct ggaagttttc
    13021 tatagctgtc aggtgacttc acttccttga tccgatagac cccttccaac aagtctgaag
    13081 cattgaatag ggaagaatcg tttggatctg gtgccaagcc tccctcgatg atttcatact
    13141 ctcctgcact attttgtttc tcaattgaaa attctgcgcc ttcaagacgt cggctattca
    13201 tactgtcaat tttcaaaagt tccaaatcga ttggcgctag ttcatcttct aaggttactt
    13261 gtagtacacc atcgatgatt tcatagcctt ccggcaaggg agcagtttcg tcttcgatga
    13321 ccgtatcttc aatgataaac ttaccttctt cattcagtgc aaactggaac acttgatcag
    13381 atagccgata cccatttggt gcgacagatt ccgagacctc atattcacca atagacagat
    13441 gcagaggttg gtcgtaattt aaatcgacat acttttctac tcctacggca cgagaataga
    13501 agtatccgat cccaggcaaa cgtttcgatt cttctggttc taacggatta ccagtgtatg
    13561 ctcgatacag cgggaaggaa gaatccccca ctttcctgat atcgaaacct gcaccatcta
    13621 atgtatggcc ttcatggtct aattttctaa ctcggatctc ccgataatca aacgtattgt
    13681 aagcagtgaa tgctacccat aaacgatcct cttcatctgt tgtatacgtg agatattgcc
    13741 aattttcttc acctgtttgc ttgagttgga tagtccctat agcattatcg taaagtcgca
    13801 agatccatgt gtcattttca tcaggttctt tgaaaccttc tgggttctgg ctctcaatga
    13861 ttttgtattc tgcatagttg gtagtttctt cactatcctt ataaacaaat gaatctggct
    13921 ctccgattgg atgaataact ttctcaccat ccacgattac atatttcccg tcttcatcta
    13981 ctgaatagaa cgtgagtctc cctttctctt cctctacttc gttcacacca gaaagccctt
    14041 tcgctactgg atatccgatt tccagtccgt taccatctgt ttcataaagt tcaaactctg
    14101 cgccattgat tggcatatta ctgccttcaa ttagtttatt gatattcatg tggattggct
    14161 tgaattcatt tcgcatttcc agtttgacat acaactcatc atactctgtg ccttctatgt
    14221 taggctcttg tacaacagaa agcttgtgat attctccttc ataataggaa ttcggatctt
    14281 taccttccgg tccgccgact ctcgaaccag atgtatattc atctacaagc tcgtagtgac
    14341 ctaacatctc atcttcgttg gctgcatatt ttttgatttg gaattgatac gtctcatcta
    14401 ctaactgata acgtggtggt ccagcgatct ctctgatcca aaatatgtcg ctgactgctc
    14461 cagtttcatc tcctaaaatc ggcatcttcg tttcaaagat cgctattccg tccgtggtag
    14521 ttttacttgt ttcgattggt tcggcttccc cactctctgg attttggtca tataatgcaa
    14581 attcgactcc agccaacggc tcgccatttt gacccttctt gctgacttgg aaattacggc
    14641 ctttatgttc gtaacgaatg gaagaagatg ggaaatacat ccgtccagta gtgatatcat
    14701 tctcataatc ctccaggttc tgaatatagg ccatattgtt cactgtgcaa aaatttccgt
    14761 cttgagctcc cgcatcaagt tttacttggt acttgatcct gaccatttcc ccttcaccta
    14821 aagaaatcgg agaaaattta taagcttggt tttcccgtcc gacatacgtt gtcggagtcg
    14881 ccactacttc tggtggggct tgtccatgac gttcttcatc ccatatttct ggctcttggt
    14941 acttcatgcc gttttctcct ggatctagat ggtaaaactg aatatctgcc gatctgccag
    15001 ctccaaatgg tgagacatta aaaccagttt catcataaag tgtggcttgc tggatcgttc
    15061 tcttgaaggt agaagagata tcctccaagg ctgtaacaat gtcattcttg ttgttcgcat
    15121 tgtaatatcg cggtgttacg ccatcttctt ctaaatcagc aatatttttc aatacatttc
    15181 gtccaatagc atctagtcgt tgtctcccgc taacgtttcc atcgattcct aaaccaatgg
    15241 agaagatact tggcgggttc tcttgatact tgtttttcag ccaatgatga taagcaactg
    15301 tgttgatcgc agatgacttt tcttgcgaag taggactgcc attcgtattg ttattagaag
    15361 aaccatcacc aacccagtga gtcgctttat tatctccttc ttttacctct ccataacgat
    15421 agtgtacatt tacttcatct tctggacggt taaagtcaga tgcataggta actggatgag
    15481 taaaccctgc attagaagta ttcccagaaa actcctcaaa atttctgaac aaaacgtcat
    15541 tatcttcagc aatcttatct tccatcacag tccagttgct ccaagctcca ttagtgctcg
    15601 tacgataact tgactgaatc ggtgcataac tgaaggtcgg tgtcccatct cccacaacga
    15661 ttaggatttt ttctggattt ctattttctc cgccattgtc tttatacaat gtttcatagc
    15721 catttttaag ccctagtgta agcggagttc ctccgattgg tgtacgattt aatgctgtat
    15781 tctcacttcc cgtcaaacga ttgatatcat ttgtcaatgg aaagatgtcc gaactgattt
    15841 gctcatggtt attgatagct gtactattat ggtaaaaatt caccataccg attcgaatat
    15901 ccagactcgg gtcagagagc agatctttac tcatttcgtt tactgcctca atcaacgcag
    15961 catttttagt ttgactattg gtcccagcgg ttaattcact catactcgct gacttatcta
    16021 gaacaaaaac gatatcgatt ggactgattt cctttttctc gcccccgatg acatctagat
    16081 agacattgta aagggttgtt gcatcttcag gatgtagatc agatgctgtg ctgcttggca
    16141 gaacaggttc tacccatttt ttgatgtacg ccccgttata atccaagtaa ctgttttcaa
    16201 aattccgttc atttccccaa agctgaacgg tttgagctgg aacgtttcta tcttcttcgc
    16261 ctgcagttga tccattatta gtagcatagt cgtaatttcg ccagttcgct cccccgtttg
    16321 tataatcact atcatcgatc aagttctctc cgtcatatgg cgagcctttg tctccttgat
    16381 agcgttcatt gctttgtgtc caatttccta acacttttgt atagtcattc gttgtaaact
    16441 tcgggtagcg atgctgttcg ttttcaactt ctccgctagg atagatatat tcataggggt
    16501 cttgcgtagc tgttggccca agacgctcca aaataccgtt gaataactgg cgttgaatgc
    16561 gctggagtgc ttgaccgctg ggtacgattg gttcgtccag cttttccatc tcattttctt
    16621 cgctacgttg aatttcagcg gaatcttctg tatcagtttc attttcttgt aattcttggt
    16681 tatctattct ttcatcaacc accacttgaa gaggtagtta cccttcgttc acatcgggga
    16741 tcttttctgt ttcaaaaatc aatgtgccgg tttctgcttc ttttgaataa acttttccga
    16801 cataccattc ttgaccatcg atcgtctcga cttttaggtc ttctccatct tcatcatagg
    16861 aggatagacc tgttccacga acgtttttta cggtaccaat cccggcggct gccgcatcga
    16921 ttgctaaacg tattctacct tcattggcac tttccttttt ttcaaaagcc agatgccatt
    16981 caagggtttc ttctttttca atatacttcc cttcaatttt tgcatagtcc tcatctaaga
    17041 atacctcatc ctcctttgtt tctgttgact cagcagctac atccagggtc gtcatatgat
    17101 agactgactg gaaatatggt ccaaatagca aactgacagt tactaactgc aaaatcttca
    17161 attttaaccc tttcacatcc ttatactcct ttcaaaaata tttgtcttct ttcacaacaa
    17221 tcaacaaaac aatgcccatc taattataat ttaatttaat tttaatttta aatcaaatga
    17281 tatccttttt agttaaaa
//
LOCUS       DAZUKC010000052        16814 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000052 DAZUKC010000000
VERSION     DAZUKC010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.053"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(39..848)
                     /locus_tag="ACG80T_001769"
     CDS             complement(39..848)
                     /locus_tag="ACG80T_001769"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4841405.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            1141..2577
                     /locus_tag="ACG80T_001770"
     CDS             1141..2577
                     /locus_tag="ACG80T_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFS4841406.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            2747..3943
                     /locus_tag="ACG80T_001771"
     CDS             2747..3943
                     /locus_tag="ACG80T_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFS4841407.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(4005..4190)
                     /locus_tag="ACG80T_001772"
     CDS             complement(4005..4190)
                     /locus_tag="ACG80T_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFS4841408.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            4421..7735
                     /gene="dnaE"
                     /locus_tag="ACG80T_001773"
     CDS             4421..7735
                     /gene="dnaE"
                     /locus_tag="ACG80T_001773"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFS4841409.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KRQLIYLLGEDNVVFK"
     gene            7903..8865
                     /gene="pfkA"
                     /locus_tag="ACG80T_001774"
     CDS             7903..8865
                     /gene="pfkA"
                     /locus_tag="ACG80T_001774"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFS4841410.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            8939..10723
                     /gene="pyk"
                     /locus_tag="ACG80T_001775"
     CDS             8939..10723
                     /gene="pyk"
                     /locus_tag="ACG80T_001775"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFS4841411.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(10758..11660)
                     /locus_tag="ACG80T_001776"
     CDS             complement(10758..11660)
                     /locus_tag="ACG80T_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFS4841412.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            11846..12415
                     /locus_tag="ACG80T_001777"
     CDS             11846..12415
                     /locus_tag="ACG80T_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFS4841413.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            12473..12652
                     /gene="rpmF"
                     /locus_tag="ACG80T_001778"
     CDS             12473..12652
                     /gene="rpmF"
                     /locus_tag="ACG80T_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFS4841414.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            12822..14243
                     /gene="gndA"
                     /locus_tag="ACG80T_001779"
     CDS             12822..14243
                     /gene="gndA"
                     /locus_tag="ACG80T_001779"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFS4841415.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDEAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            14436..15122
                     /locus_tag="ACG80T_001780"
     CDS             14436..15122
                     /locus_tag="ACG80T_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4841416.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            15119..16624
                     /locus_tag="ACG80T_001781"
     CDS             15119..16624
                     /locus_tag="ACG80T_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4841417.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
BASE COUNT         5407 a         2945 c         3533 g         4929 t
ORIGIN      
        1 attgtcacaa cctctttttt gtggaatcta attctctatt atagtttaag tagtttttcc
       61 agataaacag ctaaaccgtc ttcctgattg ctcaaaggag tgacatcatt tgcaatgcct
      121 ttgagttggt cagtaccgtt agccatagct actccccaac ctgcataatc caacaattcc
      181 aaatcattat gctcgtcacc aaaggcaatg acgtcttttt gatcgataga caggtagtta
      241 gcaatctctt gcacgccttt tgctttttgg attccttttg aaacaatctc taagatggca
      301 gtaggtccac cccatgtccg aacatcgaca taactgccaa attgacgtgt taattcagca
      361 gatacagctc ctgcaaaacg tttgtctgtt cggacaagca aagatgtagg atttgtcgtc
      421 aaatttttag gagaaagcaa attcttctct cctggacgag aagaagcaaa aattttttca
      481 tcaaagaaat caaaactgtc aataaaaaaa gtatcacgat tttcagcagc gataaaatct
      541 aaatttagct gctttttttg cgataagatt tcaaaagcga tttcacgatt gatcaacgtt
      601 tctctttcac catcccagtg ctgattaggg atgtgtacta atgcgccgtt aaaattaacc
      661 atcggtgttg ttaattctaa ttgttggtaa aactgatggc tcattcggaa tgggcgtcct
      721 gtagcaatac tgacatgatg accggcattg attgcttttt ttagagtctc ttttgtttta
      781 gatgtaatca tcgaatcttg attgagggta gttccgtcca gatcgatggc aattaatttt
      841 ttattcaata agggtccttc cttcctcaac tagcatttgt tttagtctag catattttag
      901 gtaattaaca agagcaatca gctattccta acaaaaacta cagttatggc tatataaata
      961 gctaaacata agctaaacac gaacgttaag tattattttc gctttttttg ttaaaaaaac
     1021 gttgaaaagc gaatgaaacg tctatataat ttgaaattgt tcgatggatt tgccctattt
     1081 tacttatttt ttattattat agttttccat cagaaaaaaa ttcagggagt ggaacttttt
     1141 atggaaaagt ttttcggttt aaagaaaaac aatacaaatg tatcaactga aataatggcg
     1201 ggagtaacga cattctttgc catgagttat atcttattcg taaatccgac gattttgtca
     1261 gcatcaggta tgcccttcca agcagtattt ctagcgacaa tcattgcatc gattattggt
     1321 acgctggtaa tgggattgtt cgcaaatgta ccatatgctc aggcacctgg tatgggattg
     1381 aacgcatttt tcacttttac ggtcgttttt gggcttggtt attcttggca acaagccttg
     1441 gcaatggtgt ttatttgcgg attaatcaat atttttatta ctgtcacaaa tattcgaaaa
     1501 atgattattc gggcgattcc cgaaagtctt caacatgcga ttggtggcgg gatcggtatt
     1561 ttcgttgcgt atgtcggaat caaaaatgca ggattcttat ctttttcagc agaccagtca
     1621 gctatttcga gctctgtcgt tgaaggcgga aaagcgacaa atgttacgat aaatggtgga
     1681 atcgtccctg cgttagccaa ctttgacaac gctcctatct tattggctgt gattggtttg
     1741 gtactgacag ccattttagt tgtaaaaaat gtccgtgggg ctattcttat cggtatcgta
     1801 gcgaccacag tccttggtat ttttatgggt gttgttgatt taagttccat tgattggcaa
     1861 acgaattcgt taggtaattc atttaaagaa ttaggaacaa ctttcggtgc agcatttggt
     1921 tctgaaggca tgcaatcttt attcagtgat tcttccaaga ttcctcaagt attgatgacg
     1981 atcatcgcat tcagtttatc tgatactttc gatacgatcg gaacatttat cggtacagga
     2041 cgccgcacag gtattttctc aaaagaagac gagatcgcgt tggaagatgg tcgtgggttc
     2101 aaaacaaaaa tggataaagc actatttgcc gatgcgatcg ctacttctgt gggggctatc
     2161 tttggtacat cgaatacaac aacttatgtc gaatctgcag ctgggatcgg ggccggtgga
     2221 cgtactggtt tgacttcagt agtagtggca gtgctattcg cattaagtag tttattctct
     2281 ccactgatcg ccattgtacc aaatcaagca actgcgccag cattgatttt agttggagta
     2341 atgatgatgg cttcttttgc agacatcaaa tggctagata tggaagaagc tttaccggcc
     2401 ttctttgcat cgatctttat gggattgtgc tatagcattt cctatgggat tgcagcagga
     2461 tttattttct atacaattgt gaaagtaatc aaaggaaaat acaatcaagt ttctgttgct
     2521 ttgtggatcg ttgatatttt attcattatc aacttcatca ttctagcaac aatttaacta
     2581 aagagaaggg attggaacag tatttgttct gatctctttt tgttttacat aaaaagaacg
     2641 ttacatataa aatgaccttt gcgggatttt tctcttttta ctggcgagat cgttgcttgt
     2701 ttattcgaaa ataggtatga taaaagagac gaaagaggga tgatagatgg agattaattg
     2761 gagaaaaaat ttagtgattg cgtggattgg ctgttttttc actggtgcaa gtattagttt
     2821 agttatgcca ttcataccag tttatgttga gcaactagga acaccaaaag atcaaatcga
     2881 attgttttcg ggactagcaa tctctgtgac tgcttttgca tctgccgtgg ttgcaccaat
     2941 ctggggaaat ctagctgatc gaaaaggccg aaaactcatg atgatccgtg cagcagcagg
     3001 gatgacatta acaatgggat ctttggcttt tgtaccaaac gcgtattggc ttttgatcat
     3061 gcggttttgg aatgggattt tgtctggata tattccaaat gccacagcga tgattgcttc
     3121 acaggcacct agagaaaaga gcggatgggc gttaggaaca ttgtctaccg gagcgattgc
     3181 tggaaatctg atcggacctt caatgggcgg tgcactggct cagtggtttg gtatggaaaa
     3241 tgtattcttg atcacagggg ctttgttgat gatcacaacg gtattgacca ttttcttagt
     3301 caaagaagat ttccatccaa tcgagaaaaa agatctgata agcaccaaag aaattttttc
     3361 aaaaatggat catctctcta ttttgatcgg tctcttcatt acgaccttga ttttacagat
     3421 aggaatcact acgatcagcc ctattttgac cttatatatt cgtgaattga gcggaagtac
     3481 agaaaatatc ctttttgtca gcggcttgat tgtttcgata gctggtgttt ccgctgtctt
     3541 ttcttcaccg aaactaggaa aactagggga taagattggg aatcaaaaag ttctaattgg
     3601 aggactcatt ttatcatttt gctgttattt acctatggca ttcgtcacta caccgctcca
     3661 attaggtata cttcgcttta ttttaggctt ttcaactggc gctttgatgc cttctgtcaa
     3721 tacactgatc agtaagatta cgcctcaaga aggagtcagc cgaatctata gctacaatca
     3781 gatgtttagc aactttggac aagttttagg tcccatgcta ggatcgacag tcgcgcatgc
     3841 ttataattac agtgctgttt ttattgtgac aagtttgttt gttctgtcaa atattcttct
     3901 ttctttattc aactttcgta aagttcttcg cagcagatta taatggtggt gaggagtaga
     3961 aaataaaaaa gagcttgaga aatctcaagc tcttggctgt ttttttacga atgggcagag
     4021 gtttcttttc ttggcgcaac tgcaccaatc aaaaggataa tgacacccgc aatgaatgaa
     4081 atgattgttg tcaaagtaaa atcatatgtt ccgcttgtta atgcagcacc aatataaccg
     4141 actacttgac ctagaacgac agcccagaat aatgtaacga tatatcgcat aaagaacacc
     4201 tcttttctat cttaatttta ccagattttc tttgaatgta aagaaacgag accaaatctc
     4261 aataaaattt gagttcttgc gaaagaactc attcctacag attacaagca agcaaatgaa
     4321 gtggaataat caacttcagc gcctgctatc ttaatgaaag taaaaggatc aaacctttta
     4381 acgctagtga attttaacga aaaaaaaggc ggaatgcaag atgtttttcc cacaacttta
     4441 tacaattact tcttattctc ttttacaaag tacgatacgt attcgagaat atgtacaaga
     4501 agcaaaaaaa caaggctaca cttcattagc cataacggat aaagatgtct tatacggtgc
     4561 acctgaattc tatcgtattt gtgtagaaga aggagtacag ccaatcattg gattacatct
     4621 tagttatact tatgaagaaa aaaattatgg cttgcttgcg tacgcaaaag atgaaatagg
     4681 ttatcagcag ctgatgaaac tttccagccg aaaaatgatc caagaaaaat tggaacttgc
     4741 agaaatgaca gaaaccgacc atctagcgtt tgttgttccg gcaaatcatt ctgtttctga
     4801 ggctttcaat aaagaggata actttttcgg tcattggcag tttttacttg aaaatgtttt
     4861 tactagaaat ttttatatcg gcttgacaga aaacacgcct gatcaattgc ctgaatggta
     4921 tcagtatgtg ataaaagaaa attattcgct ttgtgcatta catcccatcg ctagtttaca
     4981 acaagaagaa ctttttgcac ttgaagtgat gaaacatatc aaagagggaa gccaaatacc
     5041 tttagaaaat ttatctgcat ctttaccact aacagcaaat gtctatcttc tttcagaaca
     5101 agaaatgatc aaacgattcg aaaaagcggg tctttcccaa gctgtaatag gcgcccagcg
     5161 tttagctgaa tcgatttcat ttgaattcca gttccatcag aaattacttc cgcattatcc
     5221 agttcctaat ggaaaacaag ctggagaata tttaagagag ttatgctatt cgcaagtgga
     5281 acgccgaatc actgactata caaaggaata ccaagaacgt ttggactacg agttatctgt
     5341 catccatcaa atgggattcg atgattattt cttgatcgta tgggatgtaa tggcttttgc
     5401 tcatagaaac aaaatcgtta ccggtgcagg tcgtggttct gctgcgggtt cattagtagc
     5461 ctatgtgtta gaaatcacag atgtggatcc attagaatat gatttgctgt tcgaacgctt
     5521 tttaaacccc gaacgcttta cgatgccaga tatcgatttg gatattcctg acaatcgacg
     5581 tgaagaagta ttgaattatg ttcgagaaaa gtatggacaa tatcatatgg cacaaatcgc
     5641 aacatttggc acaatggcag caaaaatggt tcttagagat gcagctagag tgtttggttt
     5701 atcgcaaagt gaagcaaacc gctggtcaaa agcaataccc aatcaattga aaatcacatt
     5761 gacccaagct tataaaactt cgcatcaatt agtagaatta gttcaggcaa gcgaaaaaaa
     5821 ccaactttta ttcaaaactg cagtgatttt agaaggattg ccaagacatg tttcgactca
     5881 cgcagcaggt gtagttatca gcgataagaa tctacttgaa ctagttccgc tccaacgcgg
     5941 ttctgatgaa gcttttctga cccagtatac gatgaatgag gtagaagcaa tcggtctttt
     6001 gaaaatggac tttttaggac tacgcaactt atcgattatt gattttactt tgaaagctgt
     6061 taagcgagta gaagggtatg agatccgatt aaaagagatc ccgctaaatg atgcaaaaac
     6121 gctgctcttg tttcaacgag gcgaaacctc aggagtattt caatttgaat ctgctggtat
     6181 ccggaacgtt ttacgaagat tagatccaga aaacatcgaa gatattgccg cagtcaatgc
     6241 attgtatcga ccaggcccta tgcaaaatat agataccttc attgccagga agaaaggaaa
     6301 agaagccatt cgctacccag atgattcact gatcccgatt ttgaaaaaca catatggagt
     6361 gatcgtctat caggaacaaa tcatgcagat cgcctcgact atggctggat ttacattagg
     6421 gcagtcagat attctgcgac gggcagtgag taaaaagaaa aaagacgtat tagatgaaga
     6481 aaagtcgcat tttgtatcag gagcattaca acagggatat tcggaaaaaa cagcagcgac
     6541 cgtatatgaa tatatcgaaa aatttgcaaa ctatggattc aatcggtcac atgcatttgc
     6601 ttattcattc gtgggattcc aaatggcgta tctaaaagcc catcatccag gtgctttctt
     6661 taccagcctg atgaattctg ttcggcataa cacatcaaaa ttgaaagaat atattgcaga
     6721 agcacgtaaa aataaactca aactttcctc gccgtccatt aatcaaagta attccagttt
     6781 tgagttggtc aataaggaag aaatcagatt cggactgaca gcgataaaag gtattcggcg
     6841 tgattttgta gaagatatat taaacgaaag aaaacaaaat ggcccgttcc agtcagtgga
     6901 tcaattcctc atacggatcg ataagcgttg gctgaaatta gagttactgc aatcactgac
     6961 agcagtagga gtttttgatg aattatctcc taacaggaaa caattgatgc ttgatctcga
     7021 aggaaaaatc caaaatatcg tttacagtgg tggaagtctt gacttgctgg gaatcatggc
     7081 attaaaagaa gaagaagtcg ctgactatac actagaagaa cgcttgaagc tggaagagga
     7141 gtacttaggt atttatttgt caggtcatcc tgcagaaggg tttgatcgtc ttcgtttagc
     7201 taaacaagtt cagctgatta cagatatcgt ccctaatcaa ccagtcaatt tattagtgat
     7261 gatcaaggaa gttagagaaa ttcggacaaa aaaaggagaa ctgatggcct ttgttgaagg
     7321 aaccgacagt agtggagaaa tttctgtcac agtttttcca actctttatc gtaaatatcg
     7381 taatttgttt gccgttgatc gagtgatctt tgttcaaggg cggagcgaaa tcagcaaata
     7441 taatcaggaa ctgcaactaa ttggagaaag ctttgctgat cctaaccagt tagaagccca
     7501 gtatccttca caaacttgct atttgcgtat tacagaagaa cgggaagatc ccgagataat
     7561 gaaacaatta caagtgattt ttcagaaaca cccaggatat ataccagtag ttatgtatca
     7621 cgaaaaaacg caacgaaaag tagttctgtc agatgcttat tgggtggatg acagcaagag
     7681 tataaagcgg caacttattt atctgcttgg cgaagataat gtggttttca aataacttac
     7741 ggtaaaaaat actacttgct ttcatttttt ttcatttttt tatccgtaaa tcaagacaag
     7801 tgagaaaaat aatgttagaa tgttagtcgt ggttaacgca aaaatggttg taaacctaag
     7861 tcatacttag gataaagtat tttaatgagg tgaatgttta ttatgaaacg catcggaatt
     7921 ttgaccagtg gcggagacgc acctggaatg aatgcagctg ttcgtgccgt tgttcgtaaa
     7981 ggtatttacg aaggactgga agtatacggg atcaattatg gttttgccgg tttagttgct
     8041 ggagatattc gtcgtcttga tgtggcagat gtaggtgaca agatccaacg tggtggtaca
     8101 ttcttgtatt ctgcacgtta tccagaattt gcaactgaag aaggtcaatt aaaaggaatc
     8161 gaacaattga aaaaattcgg aatcgaaggt ctagtggtta tcggaggaga cggctcttat
     8221 catggcgcaa tggctttgac aaaacgcggt ttcccagcag taggtattcc tggaacaatc
     8281 gataatgata ttcctgggac tgatttcaca atcggttttg atacagcaat caatacagta
     8341 ctagaatcaa ttgaccgtat tcgtgacaca gcgacttctc atgttcgtac attcgttatc
     8401 gaagtaatgg gacgcaatgc aggagacatc gctttatggt caggtgtggc tggcggagcg
     8461 gatgaaatca tcatcccaga acatgatttt gatatggctt cagttgctaa aaaaatccaa
     8521 gaaggccgcg atcgcgggaa gaaacattgc ttgatcatct tggctgaagg tgtaatgggc
     8581 ggaaatgaat tcgctgagaa actttcagaa tttggtgact atcatacacg tgtatctatc
     8641 ttgggacacg ttgtccgtgg tggagcacca agcgcacgcg accgcgtgtt ggcaagtaaa
     8701 ttcggtgctt acgctgtcga attgcttcaa gaaggaaaag gtggattatg tgtcggtatc
     8761 catgacaacg aaatcgttgc gtctgacatc attgatacac ttgaaaacaa caaacataaa
     8821 ccagacttat cactatatga tctaaataat tcactttcat tttaagagtg aaagaaacca
     8881 agctatttaa gctgtgtaca gctttaaata aacaaaattg aatgggagag ttttttaaat
     8941 gaaaaaaacc aaaatcgtta gtacattagg accagcaagt aactcagtag aaattatttc
     9001 tcaattgatt gaatctgggg caaatgtctt ccgctttaat ttttctcatg gtgaccatga
     9061 agaacaatta tcacgtatga ctatggtccg tgaagcagtt aaaaagacag gaaaagatgt
     9121 cggtattctt ttagatacaa aaggagctga aatccgtaca actgttcaag gcacaacaga
     9181 agctgatttt ggtcgtgcag gatacatcaa atttgaagta ggagatcaaa cacgcatctc
     9241 tatggatcct gaacatgtag gttcaaaaga aaaaatcgcg gttacttacc caggattatt
     9301 tgacgatgtt cacgttggcg gacacatttt atttgatgat ggtttgatcg atatgcaaat
     9361 cattgaaaaa gacgaagcaa accgtgaatt agtcgtagaa gttaaaaatg caggtatgct
     9421 aggatcacgt aaaggggtaa atgcaccagg cgtttctatt agtttgccag gtattactcc
     9481 aaaagatgct gacgatattc gttttggttt agacaacgat atcgacttta ttgctgcaag
     9541 ttttgtacgt aaagcacaag acgttctaga tatccgcgaa atcttagaag aaaaagacat
     9601 gactcatgtt caaatcttcc ctaaaatcga atctcaagaa ggtatcgaca acattgacga
     9661 aatcatcaaa gtttctgacg gtgttatgat tgcccgcgga gatatgggtg ttgaaatccc
     9721 agctgaacta gtaccaatgg ttcaaaaacg cattatcaaa aaatgtaatg ctgctggtat
     9781 cccagttatc actgctacac aaatgttaga atcaatgcaa gaaaacccac gtccaacacg
     9841 tgcggaagct tctgacgttg ctaacgctgt atttgatggc acagatgcaa caatgctatc
     9901 tggtgaatct gcaaacgggg actacccagt tgaagctgtt tcaacaatgg cacgtatcga
     9961 ccaagaagct gaaaatgcat tatcagaatt aggtacattc caattgaacg aattcgataa
    10021 aacagatgta actgaaacaa tcggtttatc tgtagctcgt gctgcaaaaa atctaggcgt
    10081 gaaaacaatc gttgctgcaa ctgaatcagg atacactgca aaaatgattt caaaatatcg
    10141 tccagatgca gatatcttgg ctgttacttt tgacgaacgc acaaaacgtg gtttgatgtt
    10201 aaactggggc gtttacccaa cagttgctga aaaaccatca acaactgatg aaatgtttga
    10261 actagcagct aaaaaagctg ttgaattagg tttcgcatca gaaggcgatt taatcttgat
    10321 cactgctggt gtaccagttg gtgaacgcgg aacaactaac gtgatgaaaa ttcaaatgat
    10381 tggttctaaa ttattagaag ctcaaggcgt tggacaaaaa tctgtcgttg caaatgcagt
    10441 tattgcttca tctgctgaag aagctgtaaa aaaagcaaaa gacggaatgg ttctagttgt
    10501 accatcaact gacaaagaat atatgcctgc aatcgaaaaa gcagctgctg ttatcgttga
    10561 agaaggcgga ttgacttctc acgctgcagt tgtaggaatt gcacaagata ttcctgtaat
    10621 cgttggtgct aaaggcgcaa caacaacaat tacagaaggt gaattgatta ccgttgattc
    10681 acgtcgcggc atcgtttacc gtggcgaaac aacagctatc taatttagct taataaaaaa
    10741 taaaaacagc tcttctttta cgaagagctg tttttttgtg ctgtaacgaa ttcatggata
    10801 acttggacta cttgttcttg aagaggcgtg acgatctgct gtgttcgttt tgccaatgaa
    10861 atcaagaagc gaggctgaac ttcatctgaa agagggaggc aaactaaatg atcttcagga
    10921 tggacagcaa tctctgccaa aaaaccgata cccacatttt ctcggaccat actctttaaa
    10981 atcgtcaagt cattgctttg ataaacaact tttggatgag tatgtgtttg tttagaaagc
    11041 ttagtgaatg ctgcggggtg gacataatgt tcgttcaata gtacaaactg ttcatctgct
    11101 atatctttga aagagacagt atcctgcttg gaaagaggat gtgtaggtgg cacgacgatc
    11161 atgaaacgtt tgtccaaaag taactctgtt tctaaatatt gatccacgat tggctgtgtt
    11221 gagcctaata cagctaaatc gatcctccct tgtcggatca tcccgtataa gtcccgagag
    11281 cctccatcgg taatctcaat ttgattcatc aattcatgct tgataaagta acttgacagt
    11341 ttaggaaagt attcattgcc gataatagga ggcatgccaa aacgaagctt tttcttttgc
    11401 aactgagtga tttcagctct tgcagtagaa atctcttgga gaatggtttg tgcatggaga
    11461 aacagctgct ttccagaagg cgtgacgatt aattcgtgat gcgatcgatc tcttgtaata
    11521 agtacggtgt caaactcttt ttcaagacgt tgtaatgcat atgtaatagt aggttggctc
    11581 acacggaaat aattagccac tttcgtaaaa tttttttgtt tgacgatttg gacatagtat
    11641 tcgatatctt tcgtgttcat gaagatcatc cgctccttta atggaataat gattattgta
    11701 aatgattctg aggatgatag caaacagagg ctttctaata aagatttttc tgttttttta
    11761 tgaactgcaa ggtgtcaaga aaatttcatt gacaacatgc tttttgccct gtataattgc
    11821 ttttgttgct taggagtgat gaaaaatgaa atggtcttta ttggaattga gaaaatacca
    11881 ggagacaccg ttaacttttc atgagacgct cgacttgaaa gccgctctca tgaagcgaga
    11941 caatttaata cttgatgttg cgccagtgga agtcgaaggt ttggtctcag ttgataagaa
    12001 aggctatatc gttcattaca ctgtgcagac gactttgaca gttccttcaa cacgttcact
    12061 agaacctgtt gaactgccca tggatttttc tgtagacgaa gtattcatga cagaagaaca
    12121 attccaaagt agagatgaac aaatgtctgc ggatgagatt ttgctaattg aaggtccatt
    12181 attgaattta gcagagtcga ttgaagacaa catcttattg gcgattccaa tgcgggtatt
    12241 gacaaaagaa gaggaagcag caacagaact tccaaaaggc aatgattggg aagttgtttc
    12301 cgaagaagaa taccagcgca gaaaaatgga agaagccgag aataaaattg atcctcgtct
    12361 tgcaaaacta tctgaacttt ttgattctaa atcaaaagac gacgataaca agtaagattg
    12421 gaatatattg tgtctaattt agatacaaga ctttacaagg aggtgtagca aaatggcagt
    12481 accagctaga agaacatcaa aagctaaaaa agcaaaacgt cgtacacact acaaattgac
    12541 aatcaaaggg ttaaacgaat gtccaaactg tggtgaaatg aaaaaaagcc atcacgtatg
    12601 tgcaaattgt ggttactatg atggtaagga cgtaatgtca aaagaagctt aatacatttt
    12661 aattagaaaa tgtaaaacct caaggctaac catggagcct tgaggtttat ttttttgtag
    12721 tcggtaaata aaaaatatgt tgttctttag tttgcaatgt agccgtgtta agctataatg
    12781 taagagatta gggagaaatc ccgtatgtag gaggagttca tatgtcaaaa caacaaatcg
    12841 gcgttgtcgg tatggccgtt atgggtaaaa atctagcctt gaacatagaa agccgcggct
    12901 attcagtggc tttattcaat cgtacagggg ctaaaacaac cgctgtagta gaagaacacc
    12961 cagataaaaa tttcaaagca acatatacaa tcgaagaatt tgtagaatct atcgagaaac
    13021 cacgtcggat cttgctaatg gtcaaagcag gaccggcaac agatgcaacg atccaagagt
    13081 tgcttcctca ccttgataaa ggcgatattt tgattgacgg aggaaataca ttctttaaag
    13141 atacgatgcg tcgtaatgaa gagttagcca actctggcat caactttatc ggtacaggcg
    13201 tttccggtgg tgaagaagga gctttaaaag gaccttcaat catgcctggt ggacaaaaag
    13261 aagcctatgc gctagttgca ccaatcttag agcaaatttc tgcaaaagca gaagacgaag
    13321 caccatgtgt cacatatatt ggcccaaatg gagccggaca ttatgtgaaa atggtacaca
    13381 acggtattga gtatggagat atgcaactga ttgctgaatc ttatgactta atgaagaata
    13441 ttttaaacct ttcagtagaa gaaatggctg atatctttaa agaatggaat caaggcgaat
    13501 tagacagcta tttgatagaa atcactgctg acattctgac acgtaaagac gatgaaggaa
    13561 caggtaaacc agtagtagat gtgatccttg atgcagctgg gaataaagga acaggtaaat
    13621 ggacaagcca aagcgcgctt gacttaggtg tgccacttcc attgatcact gaatctgtat
    13681 ttgcccgcta tatttctgct tataaagaag aacgcgtaca agcaagcaag attttatctc
    13741 gtacgaacga ttttgagttc acgggagaca aaaaagaatt ggtcgaaaaa atccgagaag
    13801 cgttgtattt cagtaaaatc atgagttatg cccaaggatt tgctcaatta cgtgtcgctt
    13861 caaaagaatt tgattgggac ttgccatttg gtgaaatcgc aaaaatctgg cgcgctggat
    13921 gtatcatccg agctcgattc ttgcaaaaaa ttacggatgc ctatgacaaa aatcctgaga
    13981 tcgaaaattt attgctagat gattacttcg ttgaaatcac gaaaaaatat cagcaatcag
    14041 tcagagacgt cgttgccttg gctgtccaag ctggcgtacc tgtaccgact ttctcatcag
    14101 ccatcgctta cttcgattca tacagagcag aacgtctgcc tgcaaatata atccaagccc
    14161 aacgcgatta ctttggcgca catacctatg aacgtgtcga caaagaagga atcttccatt
    14221 actcatggta ccatgaagaa taatagtctt tcggctgtta ggaaatgaga aaataaaaag
    14281 aggacgaaag agctgaagtc gattgttatg agtagacttc agctcttttt taaaattgct
    14341 atccatgata aaatgactat agatttttaa gcatattatc ggtataatga tagagttttt
    14401 aaaaagccag tagaatagaa aggataaaat aataaatgag caatattcta atcattgaag
    14461 atgaaaagaa cttggcacga tttgtcgagc tagagttgaa gcatgaaggg tataacgcag
    14521 aagtccacta caacggccga actggtttag atgcggcatt aaataatgag tgggatgcca
    14581 tattgttgga tctgatgctc ccagaattga acggtttaga agtctgcaga cgtgtacgcc
    14641 aagtcaaaaa tacaccaatc attatgatga ctgcaagaga ttctgtgatc gatcgtgtat
    14701 ctggacttga ccacggagca gatgactaca tcgtcaaacc atttgctatc gaagaattat
    14761 tagcacgtct tcgtgcattg ttgcgtcgaa tcgatattga aggcgataaa aatgtggcta
    14821 aacaaactac tattacttat cgcgatttga ctatcgaaaa agaaaaccgt gtcgtacgtc
    14881 gcggaaatga agtaattgaa ctaacaaaaa gagaatacga gttgctgctt actttaatgg
    14941 aaaacgtgaa cgtggtatta gcacgtgacg ttttactaaa taaagtgtgg ggatacgaaa
    15001 cagaagttga aacaaacgta gtggacgtat atattcgtta tcttcgcaac aaaattgacg
    15061 ttcccgggga ggaaagctat atccaaactg tacgcggaac aggttatgtg atgcgctcgt
    15121 gataaagccg aaaaaatcgg aaaaaagaga actgaaaggg ccctccttga caatcaagtg
    15181 ggcctttgcg agttctttct tcatattcgt tgtgtttaca attttcgctg tcattaccta
    15241 taaatcatct atcaatctga ttgtggcaaa agaaagaaac aatgtagagc gtacgatttc
    15301 agaagtagct tctcgtttgg ctaactcgga tgaagaactg acattagtaa atacgtatcg
    15361 taatctgacg aaagcgacaa atgacggaga tgaggtcgta gatcatactt cactggtcga
    15421 aggaaactta atgaagatcg attcctttat agctgagcta gggcagccat caatggatct
    15481 ttatgtttat gatttggacg agaacttgat ttttaaaacc catgaaaaca gtcgaaacct
    15541 gattcagact acacgtaaag ttccaacgat cgttacacta gaaggaaaaa ctggcttttt
    15601 atctatccag cctatatatt ctaaaggtac aagagaaaag atcggctatg cgcaaagctt
    15661 ctatgaatta tcttctttct atgatattcg aaataagcta ttattgactc ttattgtttt
    15721 agaagttgtt tctttgatct taagtagtat tttaggtttc tttttatctt cttatttctt
    15781 aaaaccactc aaggtactca gagatacaat ggatactata cgtaaagatc cgcaatcaga
    15841 tatccacatg cctgaaatcg acacaaatga tgaattggca gatttagctg aaatattcaa
    15901 tgagatgctt gaccgtatgc gtttgtatat tgaacaacaa gagcaattcg tggaagacgt
    15961 atctcatgag ttaaggacac ctgttgcaat tattgaaggg catcttagtc tgctaaatcg
    16021 ttgggggaaa gatgatcctg agatattaga agaatcatta agcgccagca tgcaagaaat
    16081 tagccgtatg aaaagcttag ttcaagagat gctagactta tcaagagcag aacaagtaga
    16141 tgttcattat gcgaatgaaa caactaaagg aaaagaagtc acctatcaag tttataataa
    16201 tttcaagata ttatatccag actatgtgat cactttagat gatgatttga caaaagaagt
    16261 gaccctgcaa atttatcgta atcactttga acaattaatc attatcatat tggataacgc
    16321 agtaaagtat tcaactacac gaaaagaagt ccatatctct atttcttcca ctctgaatga
    16381 atttgagatc gcgattcagg actttggcga aggaataccc aaagaagacc ttaataagat
    16441 cttcaatcgt ttttatcgag tggataaagc aagagctaga acgaagggtg gaaatggtct
    16501 ggggttgtct atcgctaaac aactggttga aaactataaa ggacgtatct tagcagaaag
    16561 tgtagtaggg caagggacaa tttttagaat ctttattcct atcgcaaagg cagaagacaa
    16621 gtaaagcatg tgctttactt gtttttttat atatccaaca aagaataagg tacaaaatac
    16681 aagcaagtta gaaattcagg aatgtcatta taaaaaaaac tagcgaacaa ttaaatagaa
    16741 taacatccgt taattaaacg taagtagctt ttagatgaac gaaaaaacaa tgaaatgctg
    16801 taatattcgt gttt
//
LOCUS       DAZUKC010000053        16727 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000053 DAZUKC010000000
VERSION     DAZUKC010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.054"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(63..263)
                     /locus_tag="ACG80T_001782"
     CDS             complement(63..263)
                     /locus_tag="ACG80T_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4841418.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(715..1035)
                     /locus_tag="ACG80T_001783"
     CDS             complement(715..1035)
                     /locus_tag="ACG80T_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841419.1"
                     /translation="MKKRVLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(1538..2656)
                     /locus_tag="ACG80T_001784"
     CDS             complement(1538..2656)
                     /locus_tag="ACG80T_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841420.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPEIEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            2889..3011
                     /locus_tag="ACG80T_001785"
     CDS             2889..3011
                     /locus_tag="ACG80T_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841421.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            3127..4434
                     /locus_tag="ACG80T_001786"
     CDS             3127..4434
                     /locus_tag="ACG80T_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFS4841422.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGESRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANMFVIGWRRVAEFFGNLF"
     gene            4553..5752
                     /locus_tag="ACG80T_001787"
     CDS             4553..5752
                     /locus_tag="ACG80T_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFS4841423.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(5779..7047)
                     /locus_tag="ACG80T_001788"
     CDS             complement(5779..7047)
                     /locus_tag="ACG80T_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFS4841424.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKAEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     regulatory      complement(7102..7168)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(7210..7857)
                     /locus_tag="ACG80T_001789"
     CDS             complement(7210..7857)
                     /locus_tag="ACG80T_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFS4841425.1"
                     /translation="MEQTIKPYQYFFIKREVEQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILDHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(8265..8768)
                     /locus_tag="ACG80T_001790"
     CDS             complement(8265..8768)
                     /locus_tag="ACG80T_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS4841426.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(8891..9655)
                     /locus_tag="ACG80T_001791"
     CDS             complement(8891..9655)
                     /locus_tag="ACG80T_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFS4841427.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMALTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFMVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKENAESLNVGVAAGIVLYHFSL"
     gene            9763..10038
                     /locus_tag="ACG80T_001792"
     CDS             9763..10038
                     /locus_tag="ACG80T_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFS4841428.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEEDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            10715..11665
                     /gene="yidC"
                     /locus_tag="ACG80T_001793"
     CDS             10715..11665
                     /gene="yidC"
                     /locus_tag="ACG80T_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFS4841429.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(11844..12818)
                     /gene="lacD"
                     /locus_tag="ACG80T_001794"
     CDS             complement(11844..12818)
                     /gene="lacD"
                     /locus_tag="ACG80T_001794"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFS4841430.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDTKIPGR
                     LPDLLNIWSVKRLKEAGADACKFLLYYDVDEPAEINVQKQIVIERIGSECEAEGIPFF
                     LEILSYDATIGDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QETCYSQAEASAYFYEQSKVTEQPFIFLSAGVNATLFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATPVYVDR"
     gene            complement(12815..13273)
                     /locus_tag="ACG80T_001795"
     CDS             complement(12815..13273)
                     /locus_tag="ACG80T_001795"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFS4841431.1"
                     /translation="MTIIHEENIFLQQPLDSQEEAFKFLAKQTFCLGIAHDEKDVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSMIIVTLNKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRKAKSKTKIATILTKEIGEDL"
     gene            complement(13270..14736)
                     /locus_tag="ACG80T_001796"
     CDS             complement(13270..14736)
                     /locus_tag="ACG80T_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFS4841432.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFAGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAMSGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSFDPDHAQYNATAAMVKAVGDASMGMMVPVLAAYIAEGIAKRPGLIVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLTILPKQLDGLKAIFLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEHSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLAQGN
                     TTFMAGVSAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKMDDSKAANRTVIEKGRAGDLS"
     gene            complement(14718..15668)
                     /gene="pfkB"
                     /locus_tag="ACG80T_001797"
     CDS             complement(14718..15668)
                     /gene="pfkB"
                     /locus_tag="ACG80T_001797"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFS4841433.1"
                     /translation="MSIYTCTMNLAIDLFIETEEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAEDYLCVSGSLPRGLSPKVLIEISRICQEKGI
                     HLIIDSSDQEAMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVELGAENV
                     LLSLGEKGAILFTKDRVFRGNSPKGKVVNTACAGDTMLGAFLAGYMNRRPLDETLRKS
                     IAAGSSTAFRKGLTDFLDVEELSKQIKIREERFERWENIN"
     gene            15811..16560
                     /locus_tag="ACG80T_001798"
     CDS             15811..16560
                     /locus_tag="ACG80T_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4841434.1"
                     /translation="MDHKLSETEQYLWNFIEHHILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINVLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISQKLT
                     KKDSVIFVSLNGETKELVTAAKNCYDAEIGSILVTANHSSTLKDYCEINLVGFKSEGS
                     YFPDYEVRSRLPLQIIARILLDAYVLRMGEN"
BASE COUNT         5031 a         3506 c         2945 g         5245 t
ORIGIN      
        1 aaaatagaca aaaacgccca gacacgcaaa tgtctgggcg tttatttttt agtataggtt
       61 aattaagctt tagtaacgtt tgttgcttga ggtccgcgtt ggccttcttc aacatcgaaa
      121 gttactgctt ggccttcttc taaagttttg aatccgtcgc cttggatagc tgagaaatga
      181 acgaatacat cgtttccgtt ttctgcagtg atgaatccaa atcctttttc tgagttaaac
      241 cattttactg tacctgtttc cataataaaa atcctccttg tgtatcaaca catattttgc
      301 aattacttga atggtgtata tctggaagat gtttatttga aacaagctac cacagattac
      361 cgaacaaaaa ctactcctat agtatatcat accgtttaca aaaaagctag agtttttcga
      421 ctttttttat taaaaaaagt tttctcagcg attacataaa gtgaaaaacc gaactttttt
      481 taaaataaac gaaatattgt tcagttggtg tttttttgct ctgatgttct tctttaaact
      541 aaaaaaatta ttctttctga ctacttatcg gttctgtttc aacggtcgag gctggggaag
      601 attctttagt aggtctgatg ttctcatggt atttctgata gcctatgaca gtgaccagaa
      661 gaacaaggca aaccaatacg atcaacgaga ttagtttttc ttttttgctc atatctatac
      721 ctcataaata acaggatgat gtccatcata tttctgagta tgagtaaaag caaaagctgc
      781 agctgtagaa tgtaagaaat gccagcaact ttcataatca caggtcggac agtaccaaaa
      841 gtaaccaaat cttggctgca aagaccgttc ttctgtttca ggaatcgcag aatcaagatc
      901 tgttgtctgt acataaacat ttttgtacag acgtgtgcct gtaataggag accgctgtgt
      961 ttcttcagca aaaactggct gacttgttaa acttagggat agaaacagca aacaccataa
     1021 cactcttttt ttcataaaga cctccttatc gataagtttt tcccaattaa aatcatatct
     1081 caagaacgcc cgttattcac attttgttca taaatgatgt tttttgtatc ataatcgtca
     1141 aaaagaggtc gtgaccacgt cacaacctct tttttaaatg gtgttccaga agtagcttct
     1201 tcggaaataa gccgaaattt cacaaaaatt tgagaaataa tttttgtcaa tctcttctta
     1261 tttcttgaag ctgaccactt ctgtcacaac ctcttcctta aacggtgttc aaaagcttgc
     1321 atctgcggta ataagcccac caaacaaaaa atatgaagag ctatttttgc ttgctgttct
     1381 tattacttga tgcagaacag ctttttcaca acctctcata cacggtgttt aatcagctga
     1441 ccttcggtat tagctcaaaa aacggcaaaa tatgtgaaac tatttttccg tttgtttctc
     1501 taatactcag gtcaaaacac tgttttcaca acctctttta ataacgaaga atcgctgtca
     1561 gactcttttc atctggcgca tctttttgat ccaaaataaa cactttcccg ccattaatga
     1621 tcacatcatc tgctaatgca ttcaacactt gacgccgatc atattctatt tcaggatctt
     1681 caccgaaacc atcagctaag tttgacgtag cgataaatag atgcgacact tttccttctt
     1741 tcgcagctgg aacgatatca actaactgat cgatgaattt cttatctaag agtttattgt
     1801 attcttttga ttcttgagct gcgagttcac ttgacagctt cttcgcccct tcttcgatat
     1861 ctctaaagga tagctgcgca ggagaagcag aaatcgcaac gtctgcacaa taatagggat
     1921 ttttagcaat ttttttgaac atcgtctgat tttctggtaa tgcaaataaa taaatcggcc
     1981 attgttctgg attttcgaaa gaatccttca agtacgtatc aacagcttgg taatagttga
     2041 cccaatcgat ctctacttct tcatcttttg gatttacgcc atggaaagtt actccatctc
     2101 gggctccgcc tcctccatta gaagagaagt tcaaaccgcc acctgtcagt tcagatccca
     2161 atgcagtaac gacatcaacc ggtgcccctt ctggtagaga tacttctttt acttgtttgt
     2221 tgctgacttc atacaatttc atggagtcgc ggttcaatcc caacagataa taggaatagt
     2281 tgaactgtcc atttttgata atggccagta aataaggaag atcacttaca tagtattgat
     2341 cgtctaccgg gatactcaaa cgatggatat aggtttggtc agcagttata atgaccgcta
     2401 cactcgtagt tgcactacgc caaaacgaag gatctcctaa caaattttca attttttctt
     2461 ggaacgggga ccagtcatgt tcggaatatt ttttctcaaa acgtttcttt gcttctttcg
     2521 caaaattttt gaatttgatt tgatcttttt ccacatcttg atgggcgaca tgcgtattca
     2581 gcatgatcgt gacgaatggt cctttagcgc cttctgaagt caattgtgac aataattctt
     2641 tatccttttt tgccataaac gtacccctcc taaaaaaatc attcctttgt ttctatctaa
     2701 agtctaccac aacactattt ttccatacaa taaaacgtat ctaaaaaaat aaaacggttt
     2761 ctacggtttt aaatttagct atttttttaa gttatcttca taatttcctc aattatttag
     2821 ccccagtctt caactattct tcatgaaatg ctgataaact aaaaaaaaga ataaaagagg
     2881 tggatcttat gcatttgatt atgattttca gtttattaag tattatcagt ttatttgctt
     2941 tcagcgcttc ccgcttatta gatgcggaaa cacagcctat tcctgtaaaa gtacgtaaaa
     3001 acgaccgcta accttccata acgtgcgttc ttatcctaaa gttttttcag aaacattgaa
     3061 actctctccc ctctctgata aaattttaat ttgtagcgaa acaaattaat tacaaggaga
     3121 tcggatatgt ccataaaaaa aacacgtcta gtcctattat tggctagtct cattctttgc
     3181 cttgcttctt ttctaccagc tgttgctgtg caagctgaag atacgttcaa agtaaatgca
     3241 aaagcagctt ttgctgtaga tgcgcaaagc ggtaagattc tttatgatca agatggagaa
     3301 aaaaccatgg ggatcgcttc aatcacaaaa atcatcggtc tttacatcgt tcttgatcaa
     3361 gtaaaagaag gaaaactatc atgggatgat aaagtttcga tttcagatta tgcagaaaat
     3421 ttgagtatca ccccagattt atcgaatgtt cctttgcata aagaaaacac ctacactgtc
     3481 aaagaattat tcgactctgc aatcatccag tcagccaatg cttcaatggt ggccttggca
     3541 gaaaaaatct ctggcagcga agctaaattc actgaacgaa tgaaagaaca attgaaagat
     3601 tggggaatca aagatgcaac gatcgttaat gcatcaggac tgaataactc ttatcttggc
     3661 gaaagccgac cagaaggtac tggagaaaac gacgaaaatc aaatgtctgc tcaagatgtg
     3721 gccatcgtgg cccgtcattt gatcttggat tttccagaaa tactggacgt atctagcact
     3781 acgacacaaa tgtttggcga aaacacgcaa tcaccagtgg aaatggtcaa ctggaactgg
     3841 atgctcccag gattcgttaa ttacaaggaa ggcgtggacg gattaaaaac tggtacgact
     3901 gagcttgcag gtgcttgttt cgttggtacg atcactaaaa acgatcaacg gatcatcact
     3961 gtcgtattga atgcggacgg ccatgcggaa aacccgagtg cccgcttcaa tgaaacagac
     4021 cgtttgatgg actactgcta tgacaattgg tcagaaaaag agctcggaaa agctaacgca
     4081 agtattccta gcctaaaaac cattgatgta aaagacggaa aagaatcaac agttcctgtt
     4141 gtgcttaaaa gccctgtaaa agtatgggta cgaaacgata tggatactgg caaattaacg
     4201 atcacgccaa cacttgacaa gaagcaagtc actgacaacg agatccaagc acctatggat
     4261 aaaggtacaa aaatcggtac cgctacgatc accttggcag acgataaatt aggctattta
     4321 gaagacaaca atgcgccatc aacagagatc attacggata aaacagtgga aaaagcgaat
     4381 atgttcgtga ttggctggcg acgcgtcgca gaatttttcg gcaacttgtt ttaattgaac
     4441 aataagaaaa gcatctttcc atcggaaaga tgtttttttg ctttttctag tttaaataac
     4501 agcttttttc atctattttc gttatactgt ataagaaaaa ggagttgaat ttatgaatct
     4561 aaccagagat ctgatctttt tagttctcta tcttattggg gtattctcta tcgtttattg
     4621 gcgaaaacag aagaaggatc cttccttgtt cttttacggc agtacgtgga catttacggt
     4681 aatcttaact tattttgtcg tgttggaaat tttttattcg accttttatt ttttgattcc
     4741 attgttgttc tttagtatct ttgctttttc ttatttcaca gaaaaacgaa ggctgctgaa
     4801 tggtctttta ttcaatgttt tcctcatcag tttcggcatc tacttgtttg tcttgctgta
     4861 tgaaacgcaa aatattttcc ttggcggact gatcgccttg atcacgattc cattgctact
     4921 tgtcctttta tttggtattt atggattgat tgtttttctt ttttggaacg gggtcacagt
     4981 tttacgtaga gaatcgcatt cattagcgaa tcttttaacg ttgatactag ccatttttct
     5041 tacactgttt cttgtttttg actttttctt gttaaaatat ttgccgcaat ggataaatgc
     5101 tttgtttttc tgcgttcccc tgatcttgat ctatctattt atcgtattct acaacttttt
     5161 gactgtctca ttcttgtacc agttcaaccg gcctcgatac aatcaagact tcatcgttgt
     5221 gttaggcgct gggttgatca atggagaaac agtttctcct cttctagcca agcgaatcaa
     5281 taaggcaatt gctttttatc gggctcaaag ccgtgctaca ttaaatccgc ccatcttatt
     5341 gatgtctggc gggcaaggag cagacgaaaa agtaccagag gccattgcga tgaaacaata
     5401 tgccatggaa caaggaatcc ctgaaagaga cattcttgtc gaaaccaatt cgacaaccac
     5461 tttggagaac atgctctatt caaaggaaat catggatcag caaatgaaag ggccataccg
     5521 cgcgatcttt agttccaaca attaccacat ttttagagcc ggtctttatg cgcggcaagc
     5581 caaattgaaa gcaaatggta ttggtgcaaa aacagctttc tactacttgc ctaatgcgtt
     5641 tttaagagaa tacatcgcca tcttgatgat gaacaaaaaa agacatatga tcatttgcgg
     5701 attgcttttc gcaggctccg cattcttatc gctgattact ttacttttct aaaaaaacaa
     5761 ggtgtgacaa aacatatgtc acaccttgtt tttttctttc attctcttaa ctattggcaa
     5821 aaagagtatc ccttttttga ccgctacttg ctgagcagaa taaataccgc tgttgcctga
     5881 acacataaag ctgatcaagc agatcataaa gaagtaaaca gccgcttgtg aaccgaacaa
     5941 ttcgatcccc atcacaaagc aggcaatcgg tgtatttgtc gcaccagaaa atactccgat
     6001 aaagcccaat cctgcgagaa aaggaacaga aacatggagc aaaggcgcta gtgctgcacc
     6061 aagcgttgca ccaatttcaa acaatggtgt cacttctccc ccttgatatc ccgcgcctaa
     6121 agacaaaact gtgaagaata acttcccaat aaaatcatag acatgtgcct gtccattaaa
     6181 tgattcttga agtaacggaa gactcaatcc gagataacgt tgtgtttgca aaatcaatgc
     6241 tgctgccaca actacagccc cacctatcgt atttcgccaa acaggatgag gaatccattg
     6301 tgcataccgt gcttttaaaa agacgatcga acggctgaat acccagccaa tcaaaccaaa
     6361 agcaatccca gcaacaaaaa ctttgaagaa cagcaacata ctccattcag ggatcacacc
     6421 catttggtaa tgtaggtgag aaacaccata gctttccgtc atgaaatttg caaacagtgc
     6481 agcaaagaag ctagggaaga tcgcttctgt tcggactttc ccgattgcca acacttctaa
     6541 tccaaataat gttccagcta aaggtgtgcc aaatacagaa ctaaaacccg cactgattcc
     6601 actgataatc aagatttccc gttctgcttt atccaatcgg aacaaacgtg ctgtattatc
     6661 tgctaacgct ccccccattt ggactgctgt tccttcacgg ccaacagatc ccccaaataa
     6721 atgggtagtt atggttccaa acagtgtaag tggaataagc cgcaacggga ttttttcttc
     6781 tccaccattt ccttgatcga tcactaagtt gtttccacga ctagaaagtc cgccgtgata
     6841 cgcatagaga taagcaaaaa gcgcaccact aactggtagt agataaagga gccaaggatg
     6901 tgatagtcgg atatctgtca caactgtcaa gctatttaag aaaaaagcag acaagctccc
     6961 catgaatata ccgagaatcg ctgtgatcac cagccacttc agcatgtaca ctaaaatgcg
     7021 tactggactc ggtaattgtt tttccataaa aaattcctcc tcaataaaaa aacgcctact
     7081 aaagaaaggg gctatcccca gactttagta ggcgtcatta gctatctttc gttagcattt
     7141 aaaggcgaac accatcacct attcacttcc attaaaagat agcatatttt tctgtaaaaa
     7201 acaataaaat tatcgttctt tctttacacg ttcagcaaat tcatagaaag gagccaattt
     7261 cgttaactgt tgattacatt ggtcactatc atgacaaata taattcccct ttttcgtata
     7321 cgtgccatca gaacctgctt tcgttgtaga aagaaaaagc gaaacattcg atgttttctg
     7381 acagattgca cagattccct tatttatcgt tggtgataaa gtaccgctaa tcccttttaa
     7441 tttttcatct tcgtaataga ccatgaattt tttctgcgta cctggatcat tccagccgat
     7501 gtacgtatgt tcttgcaagt cgatttcatt ccaagctggt tgtttgagtt ttttcacttt
     7561 tcggaataat ttctcgattt gtggtttgct aggctgcata aacggctgaa cgatttgctt
     7621 cagctcctcc aatgctcttt ctgcttgcgc ttttgtcagt tttacgtcta aaactgtcgt
     7681 taaaaatgta tcgatttctg gcagttcgtg atctaatatg tctcgaatct tttcagctgc
     7741 tagtgcctga actgtttgca ccgtttttgg atcattgaca gatgagtacg catttaataa
     7801 ctgttctact tctcttttta taaaaaaata ttggtatggt tttattgttt gttccatgat
     7861 tttaaagtcc tttcttctaa ccaaagtgca tactttgtag aaaaaagtga tcttctgcaa
     7921 agtatgtgtt acctgaagat cccggactct tttaaataag atacgttttg catcttatca
     7981 tcaccccctt taatgcttat tcttctatgt acatgattca ttcgataaat aaaagtaaac
     8041 cgtatcttga acagatagtc aactgcaaac agaaaaaagg actgtgacaa aagtcgtgtc
     8101 aaagtccctt gtttcgaatg aatggtgttc aaaagcttgc atctgcggta ataagccgaa
     8161 acttcacaaa aatttgagaa gtaattttcg taaattcctt cttatttctt gaagctgacc
     8221 acttctgtca caacctcaat atggtctatt attggtttta cctgttaaat cgaggattct
     8281 ttcccaaagt aacgttgcca tctcttacgc atacttgcag acatgggctg tgccgcttct
     8341 ttgactgcgc agtatttgtc cacgaatgtc acgatatatc cttctttata cttaggcggt
     8401 gcgatcgttg caccccacat gtgcttcaaa atgatatctc gctcaagatc cgacaaatcg
     8461 gtgatttttt ctgcattctt tacagcaatt cttggatgga tatacgcatg tgtcccctcg
     8521 tcaaatttcg tcgtacgcca atcatagtag aacagatcat gcaaaagtcc agcacgcgct
     8581 gttgctttag cattgccacc ccattttttt gcgagcagat aactatagta agaaacactg
     8641 atggaatgct ccaaacgagt ggaatgaaca tgctgtgtgt aattcgctaa ttttttcact
     8701 gcttctgttt ctaaaagatc ttgtacataa gacatatatt cttcgtcttc acgccatttt
     8761 tctgtcataa ttatcgatct gccacctttt tgaatttcat tttcttctga aattgatcta
     8821 agtataccac cagtgttcgg attattctag tcgctctaga taaaatagct aaagcttaat
     8881 agttccttaa tcataaagaa aaatgataaa gaacgatccc tgctgcgact ccgacattca
     8941 atgattccgc attttctttc attgggatat atacgttctg atcagtcaaa gacaagattt
     9001 cctgacttac tccctgacct tcgtttccca tgattatagc gaaatcctct gttttttcta
     9061 ctttgtctaa tgcatgtgct tcttcattta attctgtacc gtacaccata aaccctttgc
     9121 ctttcattac agggatcact tctgccaaat ctttttgtaa cacaggcaaa tgataattgc
     9181 ttccctgcat gcttcgcagc acctttgtac tataaatatc tgccgttcct ttacctaata
     9241 aaacaccagc cataccaaac gcatctgccg tacggatcat cgttcccaca tttccaggat
     9301 cctgtacatt atctagtaaa agccagcctc ctgaaaaatc tggcgtatct tcagatgtta
     9361 acgccatcac tgctaagatc ccttgcggtg taggtagatc tgacatagcg gtcatgacct
     9421 catctgaaac tagaaaagta gaaatctctt tgtttacagc cagccagtct ccccattctt
     9481 tcatacctct ctgtgtcaca aaaagttctt ttatttctgc atcagacgac gcagcttctt
     9541 cgatcagatg gaacccttcc aaaagatatt ctgccttttc ttctctgtat ttctttttat
     9601 gtagtttttt taattctttg attcttggat tttttgctga ttggatctct ttcacattcc
     9661 ttcacctcac ctgattatat catggaatag gtgcttgagc ttgtttcatc ctaaaaaaat
     9721 cggtatcatt gatttataaa acaggaaaga aaggtgacga acatgcgcaa agttaaaatg
     9781 aatgtccaag gacgtgtcca gggcgtggga tttcgctata tgaccaagat ggttgctgat
     9841 cagttaggcg tgaccggaac ggtaaaaaac gaagaggacg gctcagtcac aatcgaagca
     9901 atcggaaatg acgacatcat ccaaaaattt atcgaagaag tcaagaagtc tccgagccca
     9961 agtggacgag tgcagtatgt ggatgtccaa gaagatccga tgattgagga acgaaagaaa
    10021 tttgatgtgg tgggataagg ttgtgaaaag gtcgcttagt tttgagtatt aaggaaaatt
    10081 ttgtgaaaat gtctttccac atttttgcat aaatttgact taataccgag aaactgactt
    10141 ttgaacaccg tcgataaggt tgtttttgaa gcgctttgcc tcgagtatta aggagaaaca
    10201 ttttcaaaat agcttctcat attttgaaat gtaatgactt aataccgaga ggcagctgat
    10261 aaaacaccgt caataaggtt gtgaaaaagc tggtctgcat caagcattaa ggactggttg
    10321 tgaaaaatag ctctccatat ttttcaacag gctggactta atgccgtaga tgcaagcttt
    10381 tgaacaccat cgataaggtt gtttttgagg cactttgcct caagtattaa agacaggtag
    10441 taaaaaagct gatctataac ttgcaatgaa tttgcttaac ccatattttc cagacagaac
    10501 aaaaaagttt ttagtttttt aaataagaaa gaaacacttg gaatcactct tttctttcta
    10561 agtatttctt tcttgatttt ttgtcttttt tcaaaatttt tcgctacgaa aaagaaagaa
    10621 tatttatatg aaatttctat gaagttgatt gaattccatg cgctttttta gtatagttaa
    10681 gtttgtgtaa aaattataga agaggaagta ttctatgaat aaatggaaaa actggttatt
    10741 gggatccgga cttgtcagta tcctgctctt tttatctggc tgcgtgcgga tggattcaaa
    10801 cggaaaccct gacacttcag gaatcgttta tcgagtactc gtccatccta tgggccaagc
    10861 gattacatat ctagttgaga atttcaattg gtcttacggc tgggcagtta ttgcaatgac
    10921 tgtgatcgtt cgtatcatca tcttgccact aggtatcagt caatcgaaaa aaacaatgat
    10981 ccaatctgaa aaaatgcaag cgttaaaacc acaagttgaa gcagcacagc aaaagctaaa
    11041 agctgcaaca acacgtgaag aacaaatggc agcacaagca gaaatgcagc aagtatatcg
    11101 cgaaaacgga ttgagtatga ccggcggaat cggctgtctg cctctattga tccaaatgcc
    11161 gatcttctct gccttgtatt ttacggctcg ctacacagaa ggtatccgtg aatcatcttt
    11221 ttacggcatc gatctaggct caccaagtat ggtgcttgtt gccattgcag gggtcgctta
    11281 tctgcttcaa ggatatattt caacaatcgg tattcctgaa gaacagaaaa agacgatgcg
    11341 tacgatgctg atcgtctctc ctgctatgat cgtcttcatg tcgatcagtg cgccagccgg
    11401 tgttacactt tactgggtag tcggtggtgt gttcagctgt ctgcaaacat tcatcacaaa
    11461 cgttatgatg aaaccacgtt tgaaagcaca agtggcagaa gagttgaaac aaaacccgcc
    11521 aaaacaagtc gtcactccaa gaaaagacgt gacagaatca gcaaagccag ctaacaacaa
    11581 acaagcaaca aaacaaatca aacaaccaaa aaacagtaca aacaacaaag gccgcaacgc
    11641 aggaaagcag cagaagcgga agtagatagt aaataagagt gtaataaaaa ttcattaaca
    11701 gttttagcaa ttaaaaatat cgaccaagat ttttgaaaaa gaaatctggt cgatattttt
    11761 atttaagtga atgtttaatt tgaatcatcg attgatcgct atctaattgc agcagcatct
    11821 ttttatgaag aaacaacttt ttcttatcta tcaacgtaga caggtgttgc tgtttcttct
    11881 aatagtgtac ttaattcctt aatattttgt tttccttctt ctaacatcca actactcgct
    11941 ttttcttcgc cttcggtaac aaaaggcgcc actcccttcg cccatgttgc tcgcccacat
    12001 aagacaccat tgaatgtcga acctgattgt tttgcaaaac gtaatgtatc ttgaaatagc
    12061 gtagcattga ctcccgcact taaaaaaata aacggttgtt cagtcacttt cgattgttca
    12121 tagaaatacg cggatgcctc tgcttgacta taacaagtct cttgcccata tccttccaca
    12181 aatctcatat tcactggcac ttctactttc aatacatcca cttgataacg ttcttttgaa
    12241 aattctcgca tggcttcaat aactttatgt ggttttactt ttgcataggc aagagaagac
    12301 ggatcgccaa tagttgcatc ataagagagg atctctaaaa agaaaggtat gccttctgct
    12361 tcgcattctg aaccaatccg ttctataact atttgttttt gcacattaat ctctgctggt
    12421 tcatcaacgt catagtacaa taaaaattta caagcatccg caccagcttc ttttaacctt
    12481 ttcaccgacc aaatattcaa taaatcaggt aatcttcccg gaattttcgt atcatagccg
    12541 gttttttcat aagctagcag caagccacat tctttctctc gaagcgctgc tgcaggcagg
    12601 ccgtattctg gatcaagtaa aatagctgag gcatagggag tcaggtattg agaaaccatc
    12661 tgtttaaatt ggatgatgtc ttctgatgta gcttctttat attggttgat cattttcttc
    12721 aaagcaccac gctggtcgat tgctaacgcg ctaataattc cagcttcatc cgtcaatcgc
    12781 ttcaaacgtt cctgcttttc tatactcatc ttcttcatag atcttcacct atttctttag
    12841 ttaatattgt ggctattttt gtttttgatt ttgcttttct caactgttct gtaacctctt
    12901 ttcgcatcaa tgatcgtgca acgagagaca ataatttcaa atgagtcgtt cctgcttctg
    12961 aatctggaat aaataacgca aaaataaaat caactgactg tctatctaaa ctgttccatt
    13021 caattccttt gttcagtgtg acgataatca tactaggttc gttgattgtt tgagacttcg
    13081 catgaggaat agcaaaacct gacattattc ctgtagtacc ttctttttct ctttctagaa
    13141 gtttttgata tacatctttt tcgtcatgag caattcctaa gcagaatgtt tgctttgcta
    13201 aaaacttaaa tgcttcttct tgactatcca atggctgttg taaaaaaatg ttctcttcat
    13261 gaatgattgt catgataagt ctcctgctct tcctttttcg ataactgtac gatttgctgc
    13321 tttactatca tccatcttct tgtctttatc agttactctt ttcttttgga ttaatcctag
    13381 tatcactcca ccaacgatcg aaccaataag aatcgctgta acccattgga tttttcctgt
    13441 tactacaggt aatactaaaa aaccgccatg tggtgctgga acttggacgc caaacaaata
    13501 agtcaagaca gcagcaattg atgagcctaa catcatcgtg ggtaacactc ggatcgggtc
    13561 tttcgcagca aacggaatag ctccttccgt aatatgagtt gatcctaaaa taaaattaac
    13621 caaacctgca ttacgatcat tggtatcaaa atatttacca aacaataaag tagcaaaacc
    13681 agtaatcaac ggtggcgcaa tacaagctgc tgacacacca gccataaaag tcgtgtttcc
    13741 ttgtgccaaa agagctgttc ctgttacata tgccgcctta ttaaccggtc ctcccatatc
    13801 aaacgcacac atgcagccaa caatcacgcc aagaacaagt ggattcgaat gttcaaatcc
    13861 agctaaaaag ctcatcattc cttcatttat agccttcatt ggacctgcca gcaaagacat
    13921 cacgatgcca ataaatgcta cgccaataac tggatataag aagattgcct tcaagccatc
    13981 tagttgttta ggtaaaattg tcagtagctt ttgcaaaaat aagataaaat aacctgctaa
    14041 gaacccagct aaaatacctc ctaaaaatcc agtaccgcca ttcatggcta cgattccacc
    14101 aacaaatcca acgattaacc ctggacgttt cgcgatccct tctgcaatat aagctgctaa
    14161 aactggaacc atcattccca ttgatgcatc gccaactgct tttaccattg ccgctgtagc
    14221 attgtattgt gcatgatctg gatcaaaaga atagattccc caaacggcaa atgaaatagc
    14281 aatcaatact ccgccagcta ccacgaaagg caacatatga gagacaccgt tcatcaaatt
    14341 tttatagatg ctacgaccaa aattagcacc actcatcgct tctggttcta aagtatcagc
    14401 agcttcatta tctgctaaga cagtaccttt tcctgccaat gcttcgtcaa tcagccgttg
    14461 tgcatccttg attcctgtac tgacagaaac atcaatgact cgttttcccg caaatctttc
    14521 tgaatggaca tctttgtctg cggcaataat gacagcatct gcctgtttga tttcttcagc
    14581 cgttaattca ttttcgatcc caatttgtcc atgggtctct actttgatcg tgatgccttt
    14641 tttcctagct gcttgttcta atgcctcttg cgccatgtag gtatgcgcaa ttcctgtcgg
    14701 acaaccggtt gctgcaatta gttgatattt tcccatcttt cgaacctctc ttccctgatt
    14761 ttgatttgtt tacttaattc ctccacatct aaaaaatctg ttaatccttt gcgaaaagcg
    14821 gttgagctgc ctgctgctat gcttttacgt aacgtttcat caagcggtcg tcgattcatg
    14881 tatcctgcta aaaaagcgcc taacatggta tcacctgcac atgctgtatt gacaactttt
    14941 ccttttggcg agtttcctcg aaacaccctg tctttcgtaa aaagaattgc tcctttctca
    15001 ccgagggata acaaaacatt ttctgcacct aactctacta gtcgttgtcc ataaacaaag
    15061 taatcttcat ctgtaaccat ttttcgtccg aaccaagaag aaagttcttc ctcgttcggt
    15121 ttcagcaaaa atggtcgata gggtaaacaa tccatggctt cttgatcact actatcaata
    15181 atcaaatgaa tccccttttc ctgacaaatc cgactgattt ctattaaaac ctttggagac
    15241 agccctcttg gtaaactccc agaaacacaa agataatctt ctgcttgtaa tgctcttatt
    15301 tgatttaaaa aattatggac agctgtctgc ggtatttcag gtccttgatt cactaatttg
    15361 tattcttctc ccgtttgatt gacttgtgta aatacattga tacgcgtcat accaggcact
    15421 tcaataaact ctgtttggat ttgtttctgc tgtaaatagt cttctatata ctttcctgtg
    15481 aacccagcag aaaaacctaa tgccgtactt gggaaatcta gcatctttaa tactagtgac
    15541 acattcactc cttttccgtt tgcctgaatg tcatcctctt ttgtgcggtt taccataaaa
    15601 ggatgcatct cctctgtttc aataaataga tcaattgcca aattcatcgt gcatgtgtag
    15661 atagacataa ccaatgacct ccttgactct attatgaaat acgcgaaaga gaaaatgttt
    15721 tcttaatcgt tccaacacaa agaaaacctt tacatttata aactcgtcgt gctatttttt
    15781 aactaacttt tggtgaaaga aggaaagaat atggatcaca aattaagtga aacagaacag
    15841 tacctctgga actttatcga acaccatatc ctagagatcc ctaactactc catcgtgaaa
    15901 ctaagtgaac aagcaaatgt ctcaacagcc accattgtgc ggacaatgaa aaagaaaggc
    15961 tatgaagggt ttacttcgtt taagcatcat ctaaaagaag gagaaaacaa gaacatcaat
    16021 ttttctttct tagataaagt agacaaaggt atccgccgag ccattctaaa aaatgaacaa
    16081 gaagtggtac gtacgatcaa tgtgttggaa atcggaaata ttgaggatgc gatacaaaga
    16141 atcaaatttg ctgaccgggt ttttgtcttt gcccgaggat tttcagaaat gattggccaa
    16201 gaaatgctgg taaaacttca attaacggga aaaaattgtc aaatgcatac tgatccagaa
    16261 attatcaaaa gcatcagtca gaaactgact aagaaagaca gcgttatttt tgtttctttg
    16321 aacggcgaga caaaagaact cgtcaccgcc gcaaagaatt gctatgatgc agagattggt
    16381 tcgattctag ttacagccaa tcattcctct acactgaaag actattgcga gatcaatctc
    16441 gtaggtttca aatcagaagg ttcttatttc cctgattatg aagtacggtc acgcttgcct
    16501 ctccagatta tcgcgagaat tctgcttgac gcctatgtat tgcgtatggg ggagaattga
    16561 gaagcagttt attatggttg gttacagttt aaaacatcac attttttatt ctttatattg
    16621 tatagactca cgtaaacttt ttatgtataa agttattcac aatctataaa gttactttaa
    16681 gtaacaaaaa ataattgaaa atacttaaaa tcaaaaaata tcttttt
//
LOCUS       DAZUKC010000054        16509 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000054 DAZUKC010000000
VERSION     DAZUKC010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.055"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(154..552)
                     /locus_tag="ACG80T_001799"
     CDS             complement(154..552)
                     /locus_tag="ACG80T_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFS4841435.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(549..1322)
                     /locus_tag="ACG80T_001800"
     CDS             complement(549..1322)
                     /locus_tag="ACG80T_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFS4841436.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1319..2002)
                     /locus_tag="ACG80T_001801"
     CDS             complement(1319..2002)
                     /locus_tag="ACG80T_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFS4841437.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2169..3056)
                     /locus_tag="ACG80T_001802"
     CDS             complement(2169..3056)
                     /locus_tag="ACG80T_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4841438.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3053..3922)
                     /locus_tag="ACG80T_001803"
     CDS             complement(3053..3922)
                     /locus_tag="ACG80T_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4841439.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4059..5345)
                     /locus_tag="ACG80T_001804"
     CDS             complement(4059..5345)
                     /locus_tag="ACG80T_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4841440.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5618..6196
                     /locus_tag="ACG80T_001805"
     CDS             5618..6196
                     /locus_tag="ACG80T_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS4841441.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6196..7125
                     /locus_tag="ACG80T_001806"
     CDS             6196..7125
                     /locus_tag="ACG80T_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4841442.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7122..8858
                     /locus_tag="ACG80T_001807"
     CDS             7122..8858
                     /locus_tag="ACG80T_001807"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4841443.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8921..9757)
                     /gene="pheA"
                     /locus_tag="ACG80T_001808"
     CDS             complement(8921..9757)
                     /gene="pheA"
                     /locus_tag="ACG80T_001808"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFS4841444.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9754..10263)
                     /locus_tag="ACG80T_001809"
     CDS             complement(9754..10263)
                     /locus_tag="ACG80T_001809"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFS4841445.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10256..11551)
                     /gene="aroA"
                     /locus_tag="ACG80T_001810"
     CDS             complement(10256..11551)
                     /gene="aroA"
                     /locus_tag="ACG80T_001810"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFS4841446.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11564..12655)
                     /locus_tag="ACG80T_001811"
     CDS             complement(11564..12655)
                     /locus_tag="ACG80T_001811"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFS4841447.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12672..13838)
                     /gene="aroC"
                     /locus_tag="ACG80T_001812"
     CDS             complement(12672..13838)
                     /gene="aroC"
                     /locus_tag="ACG80T_001812"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFS4841448.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13840..14904)
                     /gene="aroB"
                     /locus_tag="ACG80T_001813"
     CDS             complement(13840..14904)
                     /gene="aroB"
                     /locus_tag="ACG80T_001813"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFS4841449.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14937..15959)
                     /gene="aroF"
                     /locus_tag="ACG80T_001814"
     CDS             complement(14937..15959)
                     /gene="aroF"
                     /locus_tag="ACG80T_001814"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFS4841450.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         4776 a         3634 c         2927 g         5172 t
ORIGIN      
        1 tagcatattt tgaaaagaaa gtctgatgct tagagtagaa tttcataaaa atttactatt
       61 tcataagtat tcacgagaaa aatacaaaca aaaaagcgga aataacgctc atccgccttc
      121 ttgtttgtgt ttatttcctc ttcaaacgct tgtctatgat tcagactttt ctctatcgtt
      181 attttcatct aaaagttctt gcattccgat aatttctcga tattgatgaa tcttagtgat
      241 accaatgact ttgtctacat caatactaaa acaaatacca ttacctggaa tatcgatgtt
      301 gatgccttga ccaatcgcat ccattacttt attcgtcaaa ctatcgggta ctaatgtcaa
      361 cacaatatct ttttctggcg aaatgtccaa acctaaaaat ttccctgaat catgtacacc
      421 agaacctctt ccatgtaaga tcgttccgcc tgctgcacct gcttcttttg aaaagtcgat
      481 aacatcttcg cccattcctc cgtcaacgat cgtcacaatc atgtgaagat tcaatgctac
      541 aagatctttc atttgctttc ccccaatttt tgcaatcttt ctttttcgat tctgtctttt
      601 tcatcttttc gtttataaat atatcctagt actaaagtgg ataatattgg tgtcaatgca
      661 atcattgcaa ccacaccaaa cccgtcaatg atcggatctc ttccttcaat ttgtgcagcg
      721 attgccacgc tcattgataa aataaatgtc gaacacatcg ggcccgttgc tactccaccg
      781 ttatcaaaag ccattgctaa aaagatcttg tccacttttc gtcctaaaat aaatgctagc
      841 ccgtatcctg ggatcaagaa ataataaaga gagaatccga cgagcaaacg ccacatagac
      901 aatcctactg ctgcgccgat cccaattgaa attaccatca gcatgatttt cgatcggatt
      961 ctcccctcgc tcaattcttc aacctgtttg atcattacat gaatcgccgg ctcagcaaac
     1021 cctactaaga accccattaa aaagccaagg ggaaccagta tataattgta aggcaatccc
     1081 gcaatagcac tgcctaaatg ctgtcctacc ggtacataag ccatattgac tccgtgcagg
     1141 aaaaggatca acccaatagt cgttatcaag aaacctttca taatgtcaaa gaaacgcttt
     1201 ttgcgcatac gaaaactaat gatattcatt aaaacaaata ggattacgat aggtgccact
     1261 gccaaaatga cttccatggt aacgatttcg attccatcta tcacttgctg ccagaaactc
     1321 attggaagat cacccccaaa atcatcacgg caagaatcgg ccctaacgaa gcaataccga
     1381 ccattccaaa actgtcattt tcatttttct tttgtttgat catactagtc atccctccgg
     1441 caagggccaa gataaatgga acagttactg gaccagtagt cactcccccc gaatcaaaag
     1501 caatcggcat aaattcggaa ctggtgaaaa atgaggcgat caaaacgcct atgtagccta
     1561 tagccatcaa ttgataatag gatagcttga atacgacccg aagcagcgca aaggctagaa
     1621 atactccagt gccgacgctg acgatcccaa tcagcaacac tctgccaatc tctccgtcag
     1681 atatatcata tacttgttgt cccaaaactt gcacggaagg ttcagcaatc gtgatcacga
     1741 tcccgatagc aaatcccaaa ctgaccaaaa cggtcaaact tttctttttg atcatatatt
     1801 tccctactag atctccgact tccatcatag aataatctgc tccaaacaaa aagagcgtca
     1861 ttccgatcat catgattgcc gcaccaacca gaaatgaagc gaggtcttct ccttctaacg
     1921 gtgcaaaaac aaacgttaaa atgacaatca ggacagtcat cggcaatata gcagcaatca
     1981 cttctttaaa attttttagc atgtatcctc ctcctttact ctttaagttt accttaaaat
     2041 gaaagagttt tcgagcacaa acttaaaacg ttatcattta atttttctca acataaaata
     2101 acacacgtcc caaaagtatc tttttacttg gaaaaatgac tttgaaggaa cgcgcttatt
     2161 gtgttttttt attttacaga gccgatcatt ccttgaacga attgcttttg caaaacaaaa
     2221 aatacgatgg cagttggtaa agtagcgatg actaatccag ccatgatcat tccatagtct
     2281 ggtgtatagg aagcacccat agcagataaa acaagcggaa ccgtccgttt atcaggagat
     2341 tgcaaagcta ctaagggcca taaataattg ttccagctac tcatgaatgt gatgattgca
     2401 gccgcggcat aggtattttt agccgtcggc atatacactc taaaaaaaat acttagttct
     2461 tttagaccgt ctaatcgagc ggcttctact agatcttttg gaaaagattt aacattttgt
     2521 cgaaagaaga agatcaaaaa agcagtgcta acagatggca ggatgacaac caagaaactg
     2581 ttgattccca aaaccgttcc gttcaattgt gaaaacattc gatataaagg gatcatcaat
     2641 gccgcaaaag gaaccatcat cgatagcagt agaatattga aaattaggtc ttttcgttta
     2701 ctttgataaa tctcaaatcc atatcctgcc attgacgaaa tcagcaatgc aaacacagtg
     2761 gtcaccaaag cgatcatcgc tgaattccaa agggaacgag taaaattcag ctcattggaa
     2821 aacaaatttt gaaaattgat gatcaagtta ctgccaatct ttatttttcc cgatgtgata
     2881 tccacgggtg tattcgtcat tcccaaaacc atccagacaa aagggaaaat ggaaataaat
     2941 gataggatgc ttaatgctgt atactttaat actgacccaa ttgtcgaacg aagttctaat
     3001 gcttgttttt tgacgttggg gcgttcgatt gtttcttttt ctaccatatt tttcatcgac
     3061 tttctcctcc tactttcgtt tggattatcg acaatatcac aataaaggaa acaataacaa
     3121 aggaaactgc tgccgcatat ccaaaattag gcgtgaattt ataactcaaa ttatagatgt
     3181 attgagatag ggtggtcgtt gcatttgctg gtcccccgcc agtaatattt tgtacttcat
     3241 caaaaagctg caatgtcccg atcgtcgaag taatggaagt aaacaaaatg attggtttta
     3301 aatttgggat cgtcactttg ataaactgct gccatttaga agcaccgtca atagatgctg
     3361 cttcataaat ctccgaatca atattttgca tgcctgaaag gaaaaaaatc atattgtatc
     3421 cagtccaacg ccaagtgatc gaaagaatga tcagaactct cgcccaaatt ggatgagtca
     3481 accagccaat cggtgtgtca atcataccaa tgcccaacaa caagttgttt acaaggccag
     3541 attgagaaaa tagacttttc atgatcagag aataactgac catcgaagtg atacatggaa
     3601 gaaagatagc ggttcgaaac aaccctttat atttcaactt tttatcattt aaaaggttgg
     3661 aaataaaaag tgctagaaac aacatgatcg gtacttgtac aagtaaatac aggaatgtat
     3721 taaaaaaagc ttttttaaaa gttgcatctg tcaacatccg ctgataatta cctaacccgt
     3781 taaatgtcat attgacaggg ggaccacttt gaaaagacgt gatcaaagcc aaaaccatcg
     3841 gaataaaaac catgattgat atcaaaatga ctggcaagat taaaaaagca tgcccgtttt
     3901 tgaataaatc tttctgcttc attcttttcc ctccaattct atcattgatt atctacatga
     3961 aaagacaaga aagacaatat ctgtaaggca gacgtgatct tttttgtctt ttcgtttgtc
     4021 tgatgaaaaa gcgaagaaat tcactttttt accaccgatc aattagccaa ttgtgcttct
     4081 acttgttttt gtgtatctgc tagtaccttg tctgtctctt ctccattgat gattcgatga
     4141 agagcttcag ctacgactgc ttcaattgca taggtctcat gtccgtaatt cacttctggt
     4201 atttcagatg tccatgttga aaaatcgtca aagatctttt gaccactgta aaattcagaa
     4261 gcttcttgat aagcttcttg ttcattcgcg ctaagcatcg tagatactag tccgatttct
     4321 ttcgcaagag tgcccatcag ttcttcattc gaagcaaacg ttgcttctag gaaatctttc
     4381 gctgaatctt ctccagctac tcctttgatc acataccagc cagcgccgcc aaggttagaa
     4441 gcttgcgctt tttgcagatt ttctggaagt gcaggtattg gtgcaatctt ccattttcca
     4501 gattggtttt ctgctccttg gatcgttgaa gaataccaag cgccagttgg gctagaagct
     4561 actgctcctg attgtatcgc agttactcca gcattccagt cagaggtttg ttcgactaga
     4621 tcttctttca aaagagtggc aaacaattca agaccatact ttaaagatgc attgtcttgg
     4681 atcgtgactg tctccccatc ttcagatgta taccattctc ctgcctgttg cataatcatc
     4741 cttactcgac caaggtcaga aggattgact tcagcgattt tctttcccgt cttttctttg
     4801 acatctcgcg ctacttgaat atactcgtcc cacttcagat tctccatatc ctcttctgta
     4861 taacctgctt ctgccatcag atccgtacga tagaagtttg ctgttacgcc cgaatcgaaa
     4921 ggaacaccgt atattttatc tcctactttg tttaccgcaa atttatacgg tgcaaaatga
     4981 tcttccttta caatagcaga caaattagcg aatgcatctg gataagaagt caaatagcct
     5041 tgtatcctat aatcttcaat caagacgata ttgggcaatc cttctgtatt cccgctcgct
     5101 aaagcggtgt tcagtttctg aacgatatca tcctgagaca tcgtaacaac ttctatctct
     5161 gtttcttcgt tctcataaac tttttttgct tcattcactg ctttgatatt gaatgtctca
     5221 tcccacgccc agacagtgac tttttcttct ttgtctgctg tactactagc acttccgcat
     5281 cctgttaaga aacctgatgc tgccgctaaa caaacgactc cagcaattaa ctttctgatg
     5341 ttcattttta tccgctcctt ttccatccat tagttgttta cgtttgctac attcatcata
     5401 ccaaaatttg aaaacggttt ctttattatt cagatacttc aaaaatttct cctaaaataa
     5461 taaaaaaata caaatcaact atgacaaaag gaataatctt agcatctaga ccaaagatcg
     5521 aaatggtgag gtttctttaa aataaaaata tccttgcatt ttctcctaga aataagtatt
     5581 tttaaattac aagactattc ttaaggcggg taaaaaaatg ccaatacgcg taattcttat
     5641 cgatgatgaa cagacgattt tagatggaat gaaacagttg atcaattggt ctgagcacgg
     5701 gtttcaatta atagggactt tttccagtcc gctgaaagcg cttgattttt ctttggtaca
     5761 tgcaatcgat atcgtggtca cagatgtaat gatgcctgaa ttaaatggaa tcgaattatc
     5821 gcggctatta aaacagcaac aacctgaaac acagatcctt atcctctcta gctatgatga
     5881 atttgatatg gttaaagatt cttttaaaga aggcgtcgct gattatcttt taaagcctcg
     5941 attgagtcct gatttttttc taaattcctt aactgccgtt tcacaaaaaa tcaaaaaaaa
     6001 gcccaacaaa agcccaatca ccaatcgtta tgagcagatc tgcgagaatc tttcacagca
     6061 gattgctgga caaactcgtc cattgcttga ccctcttctt tttgaacaca ccttgttttt
     6121 ctgttatata ccgaaaaacg gccattcagt tcaaaacaag gctctaaaca aaaacagtcg
     6181 atcacccatt cattgatgga tagcgacgat tcgtttacta tttatcctgt ttctaccttt
     6241 tcggaagaat ccggctatgt aatcaatact gattccaaag atacacttag gaagtttgtt
     6301 caaaagtttc gtcaagaagt ttttccaggt gatttttttg ttgtttctga ttcttttgca
     6361 atccaagata tcaatgaaat attccaagag atcagaaatt gcaccaaagg acaacgtttc
     6421 tatcataagg gaaagaacct tgcttatcag catgaacttc ttccgttatt aaccaaccac
     6481 gagcagcaag caagtagtta tttaacaaaa atcgttcata aagactacgc tttagctgtc
     6541 aaagaacttg agaatttctt cacgacgata ttggaacagc ctattgatcc agcttatttg
     6601 aaacatgagg caatcaatcg tttctatgca ttactcaacg ctttggctga cgagcatgtt
     6661 gggaatgaag aactttcttt gctcaagatc gcccttcctt ctttattagc cgatgctgag
     6721 tatttcgaag atttttctat tcttgtcaaa gaaacacttg agaaactcac ccacttttct
     6781 gcaaatgcaa aaaaagacaa tttcgatatc ttgactttga tctacgagtt cgtccaggaa
     6841 aattatgaga aagaaatcaa tttacaactt ttatccgaaa aatatcattt ttcatatagc
     6901 tatctctcag ccatctttac tgaaaaattt ggtatcaact tttcaaaata tctaaaaaaa
     6961 gtccgtatca gtaaagccaa gctctttttg acggaaacac ggcttacttt gacggaaatc
     7021 tgtgagaaag tcggctatac cgaactgggt tatttctcca gagtttttaa agaagagaca
     7081 ggaatgacac cttctcaata ccgtaaagga acttttgtaa aatgagaaaa aaaatcaaag
     7141 agcatgaact ctttgccaaa cttctgttca ttttgttcat cggtcttttt ctgcaagcca
     7201 tcgtcatttc tttgtttatt tatcatcgat caagggatgc ctatatccag ctgttcaatc
     7261 aatcgaatga tgtggtacta aaaaagattc aatctgaatt tgaaagtttg aatgatacaa
     7321 tcgagaatac attagccgct tttgattcta atccagctgt caaaagctat ttttccaatg
     7381 atccagccca acatatggaa cagcttcagc aactaagaac aatccaaaaa atgaatgact
     7441 ctctttcaaa aatccatcca atgatcgatt acgatgtatt gatctttgga gaaaatggaa
     7501 gaacttttgt cggaaacgac atgctcactg ccgtatctgc cgattctttt tttcaatctg
     7561 cgattgctca gagagtgaat gaacgtgctg ctgatacaca aatgcttttt gcacaccacg
     7621 gattgactct tcgcgataag aaagcaccca gcgtcttctt tgtcaaaaaa ctgaaaaata
     7681 cattgaatca cgtttacgga tatgctgttt tatcgatctc atccaagcaa ctagcgaatc
     7741 tttttcaatc tgtagtaaat cctgagatat cgaagatctc cttcatgaac gaagaacaga
     7801 cgatcatcgc atcaaatgaa gaaaatacaa ttgggagccg atcaatgatt ttcaaaaaac
     7861 taacagtagg tgaaacaacg atttcaaatg gacagcagtt gacccgtctt cctctgtacc
     7921 gccaggattg tacattactc agtcaagtcg atcttcattc tttatctaac caaatgggcg
     7981 tgatttttcc gattattttg tataatatcg tgacacttgc ttttgctggc tcattggtat
     8041 ttatgtattt ggatcgacac acaaaatcga tctaccgctt gattgattcg ctaaaacggc
     8101 tagaggaaga tggcttaaaa gctactgttc ctgtacaagg aagccatgaa gtccaattgc
     8161 tagggaatac gatcaatcaa ttgctaagta atgctcagca tcattatgaa cagacgatcc
     8221 aagacgaaaa gaagaaacgt tcgcttgaaa tccaaactat gcaagcacag atacagcccc
     8281 attttattta taatacgctg actagcttga aatttttgat ttggcagaaa aaaacagatg
     8341 aagcagttca aggaatcgac agtttcatta ctttactaag aagtacgatt gggaaaaaag
     8401 aagaagtgat tccagtcaag gaagaaatca aaagcgtaca aagttacatc gatcttttga
     8461 gtatgcgata tggcgaaaga atctcggcta aaataatgat tcctgatgaa ctgcagtcat
     8521 tatctatgcc gaatatgatc cttcaaccaa ttatcgaaaa cgcctatctt catgcttttc
     8581 acaataaaac gcaaggattc atcacggtgt acggcaaaac aaaaaatcag cttcttttat
     8641 tcgaagtgat tgataacggc gatggcttcg atactgaaaa taccaaacaa aaaaatgatc
     8701 atttttcagg aatcggcatc agccatgtag atgagcggat acgcctaatg tatggcgatg
     8761 catatggctt aacgatccaa tcagtgattg ggacaggaac gactgtaacg atttgtctac
     8821 cgcttcttcc taaaaatcaa tcagaatatc aaaaataaag aatagccaag cgctgatttt
     8881 tttattttaa gttaaataaa tcaatcagta ttcttgactt tcatctacgc gtctcttcta
     8941 ctgtcagcac tggataacag cccaatattt gactttctcc tccgagcatc ttgatttctt
     9001 ggagcgcatt ctctactaat gtcattggac ggtccaacaa caaatcgatc acgaaaaaat
     9061 attctcccaa gctagttttc aatggtcggg attcaatctt acttaagttg atttcccgcc
     9121 agccaaaagc tgccagcatt ttatgaagca tccctggccg attagcaggt agtgtcaaga
     9181 tgacactcat ttttactgga gctgactgct ggctggtcat ctttcggtca ccgacgatcc
     9241 aaaaacgtgt ctggttcaac tcattatctt ggatattctc agctaagatc tccaatccaa
     9301 catgctgtgc tgtttctaat gaagcaatcg ctgctgcgtc ttctttcgga tgttctgcta
     9361 catacattgc tgcagcagtt gttgattctg ttgctactaa aggaacatta ggataatgcg
     9421 tttctaaaaa ctgttgtgat tgtgctagtg cttgtggatg tgaaaggatt tttgtaatct
     9481 ttgttgctgg attgcctaaa agctgctgct tgataggaag aacgatttca cttttaactt
     9541 cgacttctgg atgtttggaa agaagatcga ttgtgtgatg aacagctcct tccaatgaat
     9601 tctctacagg aacgacagcc agatcgactt gtttgcgaaa caaagcgcgc aaacaaacag
     9661 ggatggaagc atacgcagtc aattgttctg taggaaatag ctgacaagcc gcctgataag
     9721 tgaatgaact ttctggccct agatagctga cgttcatatg cttttgatcc tttctataat
     9781 ggtatcgatg atcttttctg gtgtttgatt ggtcgtgttg attcgatgtg tcgcactttc
     9841 ttcataccaa gaaagtcgtt gtgccaataa tgcttttatt tcttctcttg tttttccaag
     9901 agctaaaggt cgaatagacg tttcatcttg ttggatacga tcaactagac agtccgcttc
     9961 tgcctctaag taaagaacaa tcggctgtgt ttttaaaaaa cgccgatttt ctgcactttg
    10021 gacaatccct ccaccagtcg cgataatttt gtcttcattt gacagctttc ttaaaatatc
    10081 tgactccact tttcgaaagc ttttttcacc gtaatagtga aaatattcgg caatcgacaa
    10141 ttggatatgt tcctcaatct ttgtatctaa atctacatac tctttttgta aacattgagc
    10201 taacccttta ccgattgttg tttttccagc gcccatgaaa ccaatcagca aaatgctagc
    10261 catgagcttc tcctcctgac acaagttctg ctaaatcgtc aaaaaatgcc ggataagaga
    10321 ttgaaacagc ttcagctcct tctagttcgc aaggtccatc agctatcaaa gcagctactt
    10381 gcagcatcat accgattcga tgatcgcctc tgctggacac ttttgcgcca tgcagaggtg
    10441 tcggcccatg gatgatcaga ccgtctgctg tcgcttttat ctttgctccc attttctgta
    10501 attcttctgc caccgcatca attctgttgg tttcttttac ttttaattct tctgcgtctt
    10561 tgatgatcgt gattccttct gcttgagtag ccactaaagc tagaatgggc aattcatcaa
    10621 tcagacgcgg aatgatttct ccaccaatcg ttgctttctt caaatgactc gtcttgacac
    10681 ttaaatcagc cgattggttc tgttcattgt gattcgtctg ggtaatcgct gcgcccatct
    10741 cctccaacac atctaagata ccagttcttg ttggattgac cccgacattt tttaacaaca
    10801 gttggctttc cggaacaaga agtcctgctg ctagaaagaa agcagctgat gaaatatcac
    10861 ctggaacagt tatctgttga ccagttaatt tttgcggtcc ggtcactatg atcgttttat
    10921 cttccactgt gatccttccg ccaaattgac gaatcatttc ttccgtatgg ttacgggaac
    10981 gttctttttc aacgattacg gatgtgcctt ctgcttgtaa tgccgcaaac agcaaagcag
    11041 atttgacttg cgcactggct acaggcatat tgtatcggat aggtgataaa gagcgggttc
    11101 ctgtcacagt gattggtaga tactggtcat tttcttctcc ttgcaagtca gcgcccatct
    11161 cgcgtaaagg ttccatcact cgccccattg gacgtttaga caacgaagca tccccgaaca
    11221 gagtagtcgt aaaaggctgt ccagccaaaa tacccatcaa taaacgtatg gtcgtaccgg
    11281 agtttcccat gtcaattgat tcggttggtt ggacaaatga gtggctaccg cgaccatgaa
    11341 tcttgatcac ctcttcctct tcttcgatct cgacacctaa ttgttggaag gcatgtaaag
    11401 tactcagaca atcttctgcg cgcaaaaaat tttgtatagt cgtcgttcct tcagcaattg
    11461 ctccaaacat gatgctgcga tgggaaattg atttatctgc tggtatccta acagtccctc
    11521 ttaatccatg tatttgttgt aataattgca tttatccagc ctcctatttg atccagcaat
    11581 gataactggt attagacata atgattcttt ttgcttgtaa caaatcctgt tggtttttaa
    11641 aactgatttg cagtattcct aaaatatctt ccctggtctc ttggatcttc aaattaatca
    11701 aagaaactcc cgcttttccg atcagtccag ttacttctgc aataacgcca ggttcatctg
    11761 gcacatcgat aaataaatca aaaaaggcag gaatcgcgcc ttgcttatgg acaggcaact
    11821 ggtctcgact ttcttttgcc cgattgaaaa aagaaaagat ttccgattgg ttctcagtca
    11881 tgatccattc tgctatttgt gtcatttctt gttgccaatc tgccaatcgt tccaataata
    11941 tctgcttatt gctcaacaaa atatctgtcc acatgacagg atctgaggaa gcgatcctag
    12001 tgatatctcg aaagcctcct gctgccagtt gtttggctcg cggatactct tgattgaatt
    12061 gttctgcctg attgactaag ccagctgcca gaatatgtgg aaaatgactt agcattcctg
    12121 ttatacggtc atgttctctt gctgtaagag ttacatattt tgctttggtt ccgcaaaata
    12181 gttcttgcag tttttcaaca tcttttttcg tcttttccaa aggtgtaaag atgtaatagg
    12241 cattttcaaa tagtttagca tctgctgcct gtatacctga tttatgagaa ccagccattg
    12301 gatgtccacc gacaaaccga agatttttct gctcagcgga tgtcacgact tgctgcttcg
    12361 tactgctgac atccgtaatg atgacgtgat cagacaagtt gattctttca aggacatcaa
    12421 gataagctaa ggttgtacgg acgggaacgg ctaataagat aacgtctgct tgttctgcac
    12481 ctgtttcaaa atcacttgca acgtaatcaa cgatctgctg ttctactgct tgctcacggg
    12541 tttttgactg actatcccaa ccaactacta tcagttctgg attggtctga cgaatacaaa
    12601 gtgccataga agcaccaatc agtccaagcc ccacgataaa aacatgttgt gccatcttat
    12661 ttgccccctt tttagtaatg tttcaagtag tcacggtact ccttcactgc agctttcaac
    12721 tcaacaagcg aatcacttgg gaattttgac aatagctctg tcgctaccac agtggctaca
    12781 acattttccg caacgacgct agctgcagga actgctgtac tatccgaacg ttctacactt
    12841 gctttgtaag gttcttttgt atcgatatca acactttgaa gtggcttgta caatgtagga
    12901 atgggtttca tgaccccgcg aatgacaagt ggttcgccat tggtcattcc gccttcgaag
    12961 ccgcctagat tattggtacg tcggaaatag cctctttctt cttcccatag gatctcatcc
    13021 atcacttctg agcctggtaa tgaaccagaa gtgaatccga ccccaaattc tacgccttta
    13081 aaggcattga tactaaccac agcttgtgcg attttggcat ctagcttcgt gtcccattgt
    13141 acgtagcttc ctaatccagc tggaacgcct ccaacgagca cttctacaat tcctccgatc
    13201 gtatctccat ttttctttgt ctgatcgatc aaatctttta ttctttgctc aactgctgga
    13261 tcaacaactc gtacttctga cgcttcagaa atttctttga tttcttgaat cgagatattt
    13321 ccaggtattt ctgcttggat tccaccaaga caaatcacgt gtcctgctac gatgatgtca
    13381 agttcttcca gaattttctt ggcaattgcg ccgattgcta cacgcatggt cgtctctctt
    13441 gcagaggacc gttcaagcac attccgaaga tctcgatgat ggtatttcat accgcctacc
    13501 aaatccgcgt gtcctggacg cggacgtgca actcgccgca gtgattggtc tttttcaggc
    13561 acttctgcta cagacatgac agttgtccaa tttttccaat ctttattttc taccactaaa
    13621 gtgacaggtg aaccaattgt tttgccgtga cggatgccag acgtgatccg aacacgatct
    13681 ttttcgatct tcatccttcc tcctctgccg taaccagctt gtctgcgagc gagttcgtga
    13741 ttgatttctt cttctgacag aggtaatcct gctggtagtc cttcaataat ggctgttaat
    13801 tctggtccat gggattctcc cgcagtcata taacgcatct tacagtcctc ctaagtaatc
    13861 tttgaatgtc tctgtgggaa caggaacgat ttttgcttgc ccaatctttt ctagcaagat
    13921 gatattcaat tggcttcctc ttgctttctt atcatgggtt atcgcgtgga ataattcatc
    13981 tggatgactt agatcgtacg taatcggcag atggaatttt tgaagtatcc tttctaaagc
    14041 agctgtcgat ccagttggag acaagccgat tttttctgca tgagaagtga tggctaccat
    14101 tccaatagct actccttctc catgactgac taccccatat cctgctgtat tttcaattgc
    14161 atggccaatt gtatgaccaa aatttaaaat aagtcgattt ccttggtcaa agacatcttc
    14221 ttccactact tttcgtttga tttccaacgt agcagtaatg attggaacag catgttccag
    14281 aagatctttt tcgtctttca gctcgttcag ctgtttccaa agcgttgcat ctgctatagc
    14341 tgctgatttg atgatttcag cgattccttc tcgtaaacga cgtaccggca aagtttttag
    14401 aacctcagga tcaatcaata caccagctgg ctggctaaat gttccgacaa ggtttttcgc
    14461 tttgtttgta ttgactgctg tcttcccgcc aatactagaa tctacttgtg caagtagcgt
    14521 agtcggaatt tgaacgaaat ggatcccacg catgaacgtc gaggccacga atgctgctag
    14581 atcacccacc attcctccac caagtgcaag tatcccatcg ctgcgggtaa aatcattatc
    14641 tgctaaaaaa tcgtataact cagctgcttg ttctaaagat ttactggtct cacccgccgg
    14701 tacgattgaa aggactggtt caaaattagc ttgtctcaat tggaacatga ttttttctgc
    14761 atatagtgga gcaacgtttg tatcagtgat cacagctact ttttgaggtc tccaaatcgt
    14821 ttgaagccat gatccagcct gttctagtgc gcctttttca atatgaatat cgtatttttg
    14881 gttatcaaca tgtacagtaa gcattcgttt cacttctttc ttttaaaaag ctggaattaa
    14941 tcgagtgtct gaatcttttc cattgcttct ttcatgatcg caacttcttt catcatgcgt
    15001 gagtaagtct tttcattcaa cgattgggga ccgtctgacc aagcatttgc aggatcagga
    15061 tggatctcta cgatcatgcc atctgctccg cttgccacac ccgcacgagc cattggtggt
    15121 acaagttccc aaattcctgt accatgactt ggatcaacaa tcacagggaa atgagttaat
    15181 tttttcatca atgggacagc acttaaatca aacgtattac gtgtcgctgt ttcgtaagtc
    15241 cgaatcccac gttcgataaa aatgacagga gatttgtctt cgactgcaat gtattctgca
    15301 gcattaagcc attcattgat cgtaccggaa attccacgtt tcaatccaac aggtttacct
    15361 gttttaccga cagcagatag cagtttaaaa ttttgcatat tacgtgcgcc aatctgtagg
    15421 atatcgctgt actcagccac catatcgata tgcccttcat ccatcacttc agtaatcact
    15481 ttcattccaa attcatcagc cgcttgacgg atgtatttca atccttcttc ttctaaacct
    15541 tggaaagcat atggagaagt acgtggtttg aaggcaccac ctcgcagaat ttttgcgccc
    15601 ccggctttgg ccattcgcgc acattctctg atttggtcta atccttcgat agaacaagga
    15661 ccagccatcg tcacgaaact gccgtcacca atttttactc cgtctacatc cacgactgta
    15721 ttgccaggat ggaattctcg agaagtcaat ttgtatgtgt ttaaaatacg cacagcattt
    15781 tctactcctt catagcgtgt aaatgctcct tcttgtaaac ttcgcgtatc accttttagt
    15841 ccaatcacta ctcgttctgt tccttgatcg attgagactt tgaatccttc tttttcgata
    15901 tgatcgacga ctttttttat ttgttcttcc gttgcttctt ttttcataat tactatcatt
    15961 gtttttcccg ctttcttttg tttacttatc tatcttggct gattttctct actttttcaa
    16021 ggtggtttta cattaattcc tactaaatga ccgtgtcctg ttgttctcat tttcgctcct
    16081 tctttcctac ttatgaccca gataatcaac aaaaaaagat gtccctgtag gaaattgcct
    16141 ctacagtgaa catcttcaac gagccttaaa aagcgctgca aaaatataaa atagccacta
    16201 tagatgaatt tctctacgtg gctgtaagtc gtgtttaaaa atctttagcc atcatagata
    16261 caaacctggt ttgtttgtac ccacgaagtc atgaatacaa acctatctaa aatagctaaa
    16321 gtaatacgta ttcagttttg taagttttct agatttcata aaaaatcgct ccaaactgat
    16381 gtaagaattg tacctagtat agacataaaa aaatcgactg tcaatcattt ttatgaaact
    16441 ttttttaatt ttctgatagt taactttgtt tttcagaaaa ataacaattg gttttattga
    16501 agaaaatag
//
LOCUS       DAZUKC010000055        16485 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000055 DAZUKC010000000
VERSION     DAZUKC010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.056"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(8..1435)
                     /locus_tag="ACG80T_001815"
     CDS             complement(8..1435)
                     /locus_tag="ACG80T_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HFS4841451.1"
                     /translation="MMRKQSIEGRNQFAMLTIDDLVPKDHLVRKIDAAIQFDFIYPIV
                     ESTYSTHGRPSIDPVVLIKLVFIQYLFGIRSMRQTIKDVETNVAYRWFLGYSFEDPIP
                     HFSTFGKNYVRRFRETTLFEDIFSHILEQAVKAGFVTEDNLYIDSTHIKANANKHKFT
                     KEMTYGQAKAYQDELEDEINKERIAVGKRPFTWDTESELSLQKISHSDPESGYYVKGE
                     REKQFAYSAHTSCEDNGFILSTLITPGNVHDSQVAIDLVKQSKRAFPNIKRVVADAGY
                     KTPKFVHFLSHLKLYPILPYTVPHGVKGMFRKKDFVYDAYFDCYLCPNNQPLLFSTIT
                     RDGYRTYKSNPKFCRSCPLLKNCTLSTKHQKIVTRHVWADLMDELEHQRHTALNREIY
                     KKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLTFAAMNLKKLATWLWKAKKPM
                     AFYLKFLQRLTKRSWQLSHSFLSTV"
     gene            complement(1548..2336)
                     /locus_tag="ACG80T_001816"
     CDS             complement(1548..2336)
                     /locus_tag="ACG80T_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841452.1"
                     /translation="MSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWISAVGEEN
                     IEFEIKNMDDLKAKFPFIASKLSQFAEEKSWNELVSFSEKIYDITQEDECVSWEMNNQ
                     YKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYAEYLKTE
                     RDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALSQYSSDE
                     EVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            complement(2442..3242)
                     /locus_tag="ACG80T_001817"
     CDS             complement(2442..3242)
                     /locus_tag="ACG80T_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841453.1"
                     /translation="MVEISKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETLKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIEGDTYPDAKSLATSDFKT
                     FYGYLMGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYADDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            complement(3581..4690)
                     /locus_tag="ACG80T_001818"
     CDS             complement(3581..4690)
                     /locus_tag="ACG80T_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS4841454.1"
                     /translation="MKNKILLFLTLICTLVLTIYPASQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKPETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVLGALASLKVLILLIKEFRKA"
     gene            complement(4680..5537)
                     /locus_tag="ACG80T_001819"
     CDS             complement(4680..5537)
                     /locus_tag="ACG80T_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS4841455.1"
                     /translation="MKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNSYK
                     EKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGELVG
                     EILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKYFT
                     DLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVGDN
                     SHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKIVA
                     KRRGMKNEE"
     gene            complement(5612..7393)
                     /locus_tag="ACG80T_001820"
     CDS             complement(5612..7393)
                     /locus_tag="ACG80T_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS4841456.1"
                     /translation="MGLGASSISSSLGLGGQVAYAQQNYNYQGTGTIHIHKVTNTNGD
                     GNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKSLGWTDSDTTKGQHIDDAK
                     ARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYLIVETGTVKGVVEEAYPMI
                     VNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDGKALAGTVFELRKDDGSDQ
                     VVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANRTTYLLDGKKFKFSVKRGETAKV
                     VLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIGNYEKYVVKDVLGAELTYD
                     SSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFDAGDRFFTILMNAHTNSKA
                     KMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIKTDGDTSKALSGAKFVVKN
                     KIDPNKVTYLKQDPTTKAVTWIADKSKATEFVSGSDGKFQIEGLQYTYDVDFDKQGNP
                     TITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSEVTDIKNLKTPETVRTGGI
                     GTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            complement(7496..9886)
                     /locus_tag="ACG80T_001821"
     CDS             complement(7496..9886)
                     /locus_tag="ACG80T_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS4841457.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMVLSTLIMPFSAIRAEADVTKPT
                     SAKFTETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKIKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGAFKQEGITVKDDGQTFEYTATNTLKKGVVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGISVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDRNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            complement(10156..11403)
                     /locus_tag="ACG80T_001822"
     CDS             complement(10156..11403)
                     /locus_tag="ACG80T_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFS4841458.1"
                     /translation="MNMKKNSLGMVLFASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPVENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKSMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            11795..12211
                     /locus_tag="ACG80T_001823"
     CDS             11795..12211
                     /locus_tag="ACG80T_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841459.1"
                     /translation="MNKKRKLSVLITLISILTILSGILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETGTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            complement(12228..12965)
                     /locus_tag="ACG80T_001824"
     CDS             complement(12228..12965)
                     /locus_tag="ACG80T_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841460.1"
                     /translation="MADRKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(13387..13950)
                     /locus_tag="ACG80T_001825"
     CDS             complement(13387..13950)
                     /locus_tag="ACG80T_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841461.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            complement(13937..14464)
                     /locus_tag="ACG80T_001826"
     CDS             complement(13937..14464)
                     /locus_tag="ACG80T_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841462.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSDKEKIEYYKPFTFKLTNFGIKTSSSGLYEVLTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            complement(14487..15872)
                     /locus_tag="ACG80T_001827"
     CDS             complement(14487..15872)
                     /locus_tag="ACG80T_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFS4841463.1"
                     /translation="MKKSKKTFGTLLIAVMQIIGITASLITINPQVTHADGIATYDAQ
                     FNCSTEGSQGSSGSGKDSDGNETGSGAVSGDVAKNIDDIYKFMNGEYGLSGATIAGIL
                     GNWKQESGNDPRTVEGYYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVNWAKKN
                     FGGAWWTTEAQLDFMFKGDGGFVAILKDYALNASDDVVANAVAFHKNWEISADSESAV
                     VAVRGGNAKEIWEYMKSKGMDGAKDVSKINKIAGGTSTGGGSNNSSSSASGTVEDACA
                     TTDDANSGSGSMGKEGKINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFN
                     GIYQGQCTELTWAYMSQLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDG
                     WLWAVGDAGHTGVVIAVFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFS
                     GVGKAKFEIEK"
     gene            15955..16446
                     /locus_tag="ACG80T_001828"
     CDS             15955..16446
                     /locus_tag="ACG80T_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841464.1"
                     /translation="MTDKTGNLPKLAPKTSQTKSKKDEKIKNAVKKKSVKESSSLLLD
                     IPMSTFDEILDDNTDETSVKIASHVGMNSQNDSFYHLLNSLRELEDKKNQVIASKAPD
                     LTTDLDAEIIQVKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISEQKK
                     SDD"
BASE COUNT         4696 a         3314 c         2434 g         6041 t
ORIGIN      
        1 gctctattca gactgtagac aaaaaggagt ggcttaattg ccaactcctt tttgttagtc
       61 tttgtagaaa ttttaaataa aaagccatag gcttttttgc tttccatagc catgtggcta
      121 gtttttttaa attcatggca gcaaacgtaa gcatcgtgtg cgtggcgact ttttcgagac
      181 ctcgatattt cgtccaacgc ataccatgct tttcttttgc atctgcaaat attcgttcaa
      241 tggtttgttt tctcttttta taaatttctc tattcaacgc cgtatggcgt tggtgttcaa
      301 gttcatccat aagatccgcc caaacatgtc gagttactat tttttgatgt tttgtactca
      361 aagtacagtt tttaagtaat ggacaactcc tacagaattt tggatttgat ttataggttc
      421 gataaccatc acgagtgata gttgaaaata acaacggttg attattggga cataaatagc
      481 agtcaaaata ggcatcataa acaaagtctt ttttacgaaa cattcctttt actccatgag
      541 gtacagtata tggaagaatc gggtagagtt tcaagtgtga cagaaaatga acaaatttag
      601 gcgtcttata tcctgcatca gcgacaacac gctttatatt tggaaaagct cttttcgact
      661 gtttgaccag atcgattgcg acttggctgt catgtacatt tcctggtgtg attaatgtag
      721 ataagataaa accattatct tcacaggaag tatgcgcgga ataagcaaat tgtttttctc
      781 gttcaccttt aacatagtaa ccactttctg gatctgaatg acttattttt tgaagactca
      841 actcactttc tgtgtcccat gtaaaaggac gttttcctac agcaatacgt tctttattta
      901 tttcatcttc taattcatct tgataagctt tagcttgacc gtaggtcatt tcttttgtga
      961 acttatgttt attggcattt gccttaatat gagtagaatc aatataaagg ttgtcttccg
     1021 taacgaagcc agcttttaca gcttgttcta gaatatgaga gaaaatgtct tcaaataaag
     1081 ttgtttctct gaatcttcga acatagtttt taccaaaagt agagaaatgc gggataggat
     1141 cctcgaatga ataacctaaa aaccaacgat aagcaacatt tgtttcaacg tctttaatag
     1201 tctgtctcat cgaacggata ccaaataaat attggataaa tactaattta attaagacca
     1261 ctggatcaat actgggacga ccatgggtcg aatacgtaga ttctacaatg ggataaatga
     1321 aatcaaactg aatcgcagca tcaatctttc gaactaagtg atctttaggt acaagatcat
     1381 caattgttaa catagcaaat tgatttctac cttcaatcga ttgttttctc atcattagaa
     1441 tcactcccag aatagtttat acaatgagta taaaacaaaa aaactgcaaa caacactccc
     1501 aatttaggag tttgtctaca gtctgaatag agcttttcag ctctatttta ttcaatatca
     1561 ttttgatcaa ttaataatcg tacactccaa ttaaccgagt tttgatcact caaatattta
     1621 taatctttgt catcaaaaac cagttgccaa actctggtac gaacagtttc ttcgtccact
     1681 tcctcatctg aagagtattg cgataatgct tcttcaacta tatactttac aacttcctca
     1741 aaatcagtag gaactttatc aataaccact tcatagtctt cttgttttac gatattggga
     1801 tcaatatgtt catccaaaca tacctcagtg agtttttctt tcacctctgt atctctttct
     1861 gtttttaggt actctgcata gtcttcctta gaccattgtt tttcaagatc atccgctata
     1921 cttttaataa aggattcttt atttaaccaa ttatctgcgg tatctatacc ttcaaactca
     1981 aaagctgttc cttctatacg tgatttgacg aaagcatcaa ttttcttgta ttgattattc
     2041 atttcccatg aaacacactc atcttcttgc gtaatatcat aaattttttc ggaaaaacta
     2101 actaattcat tccacgattt ctcctcggca aattgactta gtttcgatgc gataaaagga
     2161 aattttgctt ttaagtcatc catattcttg atctcaaatt cgatattttc ttctcctaca
     2221 gctgaaatcc atgctgaaag attgattgca atatcgctaa agtcttctac attttcgaca
     2281 tttcctgtat tatcttttat tactgcttct aaagccatat cggttgttgc actcatggtt
     2341 tgatctcctc tcataataga gcaaaaagcc ggttaaaccg gctttttgca tggtacgttt
     2401 tgtttggtag ttttctgctt gtgtttatgt tgaaataatc cctatagctc ttcttttaat
     2461 agttcttcaa ctttatctct taactctccc tgaccactct ctaatagatt taaatagctc
     2521 atcagaatat ccgtttcata cgtttttaat tcagctctat actcactttc tgaatatgat
     2581 cgtaaatacc catcattgct aaatgcaact agatcatctt gaaagttcca ttctcctaaa
     2641 tttgttttac tcactgcttc ccatggacta ttactaaaca tttcattaaa gaaatcttcg
     2701 tcgttttgat ataccttcaa ttcaggaaaa tagttgctgt cttcattgat ttcttgcacc
     2761 atttttccaa gttcttcttc agataattta ccttccaata attttacttt ttctctgtca
     2821 tctgcataag ccatttgaac ctctcctttt tctttttcaa tttcttcagg tgtttgtttt
     2881 ggcgtaactt tcaaatctat tccattgcct attagaccca ttagataacc atagaacgtt
     2941 ttaaagtcac ttgtagcaag agatttggca tcagggtatg tatccccttc aattttatct
     3001 aaaacaaaat cagcaatcgt tacccaagga gaatatatcg tacacactac atcccatgta
     3061 gcatcttcaa tattcgtcca tgtcggaaag tcattttcat tcaaatagtc ttttttcagt
     3121 gtttcgatca gagtttttcc actttcgttt tggcttagat attcaacaaa ttctgcacca
     3181 tcaaacctgc tactaaatag tttagctaat gagtctaaag ctgtttttga tatttccacc
     3241 attactacac ctcctattaa tattttacga tgacttgttt ttcattcaca aataagattt
     3301 tctacatctt atctattgct ttatagcttt tccatacatt atttctaaat acaaccaata
     3361 accctattaa attaagcttt tgagtttatc ttttctaata atattctgat aaatattctg
     3421 ataaaaaaat ttgttgtatt tttttcttga ctttttcttt ggaattaaga tcagttttga
     3481 ttttatttaa aagtgaatct agctctattc aaaataaaaa agagatcaat aatagtatca
     3541 atcccttttt actgtactct atcaattttt ataatcttca ttatgctttt ctaaactctt
     3601 tgattaaaag aataagaacc tttagacttg ctaatgctcc taatacagtc caaatgacta
     3661 aaagcatttt ctgatgttta ttggtaattc ctgtaaatat tgaacttgcc ttttcagcaa
     3721 acgttctgtc ttgcatctct acgctacctt gttccggaat attaagatca ttatttccat
     3781 ctaattcctt gttggcacga tcaacatcct caaataaatc attagaagat gtaacagttt
     3841 ctggcttatc tgaaacggat tcactatcaa tggcatcagc gtatgcggta ttgccaactg
     3901 ttacttgtgg cgtgatgtat tttgcttttt catctcttag acttctaact aataaacgat
     3961 cgtagttctg aggatatgga tgacaagtat aaagtgttaa catatctaaa tcaggaatat
     4021 cagtcagtgt tgaattctga ccgtaatttc taataacgtc tgttcggaaa accgtataaa
     4081 ctgctttttc accaagataa tctatataaa tgtggtctcc ggctactaat tgatcaacat
     4141 aaagaaattg tggtgacgta taaccgccac gatgacctgc tattactgtt tgatgacctt
     4201 tacctgataa aggtagacta gtagcactca tttgtgccac acctctatat aaatgctcct
     4261 gtgaagcgcc tagaaacaaa agctctagca catttatttt tggaatatag acataaccaa
     4321 ccgctcctga ataggtagat ggttcaaaat ctttcgtttg attttttgaa aaaacatctc
     4381 ctgtgtatgg attgaccttt ccttcagctt cacgaatatc aaatgatcca ccgtctgttt
     4441 cctgagtatt gtctactcct ttatctgttt tctgagtatt gtctaatcct tggttaccgc
     4501 tgttcgattt tatttgttcg actttttttg ccacttcctc ttttgaagga gtttgttttt
     4561 tctcaatttt ctcttggatt tccgccacat tctcttttgt tttgaattga tgatagagct
     4621 gacttgcagg ataaattgtt aaaaccaaag tgcaaataag agtcaaaaaa agtagtattt
     4681 tattcttcat ttttcattcc tcgtctcttt gctactattt ttttaataat gactatcaca
     4741 gctattatta ataaaacccc tacaactacg ataggatacc agttcatctg cgcctttacc
     4801 tttaaatctt ctttcttctt ttctttctca acaatacttt tttggtaagg tatgctaacc
     4861 cctctaacga gtaacctatg agaattatct ccaacaggtg tacatgtcat aagtgttaca
     4921 taatcttttc ctgccacagg aagcaaatcc tcaaattcgg ttggtagaat tgtttttatc
     4981 cgatctactt gataagcaag ttttttccct gaaatatcaa taaaaaattt atcaccaagt
     5041 ttgagcaact ctaaatctgt aaaatatttt ttattaggaa tgcctgaatg agctgatata
     5101 acagaatggt ttccaatccc accagttgga taatcagttc cggttaatat tcctgctcct
     5161 gctgatagtt ggaaatcgtt tacaccatca taaattggca aatccacatt aatctttggt
     5221 ataagaattt cacccactaa ttcacctaat ttatccttct gaagatttcc atttgacgga
     5281 atattgttat tatcttcagg aatcgtgact gaggtttgtt ctgtgtcttt tcgtttccta
     5341 ttaacttctg aatatctctt gaagacttct tcattctcct tttcagaagc ttcatcatat
     5401 ttttccttat aggagtttag tgtttgttga gtttggtagt aggtataagc ctgagtaatt
     5461 acaggatata gacctactcc taaacatagc aacatcccaa tttttaagat gatcaacttg
     5521 atattcttct ttttcatgct catacctact tttcacacac tttttaaaaa gaaaccctag
     5581 agccaaaaac actctaggga attttatttc attaagcttt gtgttgttct cgtttgtaga
     5641 caacatatag ccctgttcct gccacacctg caaccactag aaatactact gttccaattc
     5701 cacctgtacg aactgtttca ggtgttttaa ggttcttgat gtctgttact tcgctagaag
     5761 catcatcatt cgataccaca aatggaacat cgtttctagt tggtgtatat cctgaaggag
     5821 caacaacttc ttcaagagca taatcattga tttttttatc tgtgattgtt ggattacctt
     5881 gtttatcaaa atctacatca taggtatatt gcaaaccttc aatttggaac ttaccgtctg
     5941 atccggaaac gaattcagta gctttagatt tatctgcaat ccaagtaaca gctttcgttg
     6001 ttggatcttg ttttaggtaa gtaactttat tcggatcaat tttgttcttc actacaaatt
     6061 ttgctccact caatgctttg cttgtatctc catctgtttt aatgaattgg tgatcaccaa
     6121 tgattgtata agcgtgatca actggtgttt cgtcttcggg ttcatatcca ttgttgtaga
     6181 tcgttgaaac atcattctta agtggtgtat ccattttagc tttactatta gtatgagcgt
     6241 tcatcaaaat tgtaaagaag cgatcccctg catcaaattg tttctgaagc ttatccatac
     6301 ctgattcagt aaagacatag tttgtcgttt gtccgtcacg ttcgatactg taatcagtat
     6361 ctaaagtcat tccttcgatc acttctgatg aatcataagt taactctgca cctagaacat
     6421 ctttgacaac atatttttcg taattaccaa tattaccagg aattgatggt ttcaatgtat
     6481 attgaacatc ttcgccagta tcaacggttt ctttatcaat taccttagta attacaggtc
     6541 gtttgtagtt ggtaacatca agaactactt tagcagtctc cccacgttta acgctaaatt
     6601 tgaatttttt accatctagt aaataggttg ttctattagc atctgttccc aaatcggttt
     6661 ctaggaaata atagtcacct aattccaaac catccacagt taatttacct gtgctgtctg
     6721 ttttataagt tccaacaacc tgatcagatc catcatcttt tcgcaattca aaaacagttc
     6781 cggcaagagc ctttccatct tcgtcttttt tatcaagctc tgctgagttt aattggaaat
     6841 agtttttagg atagacatgt acatcataat taaagtattg atcatctgat ccgtttagtt
     6901 caggtacgtt aacaatcata ggatacgctt cttcaactac acctttgact gttcctgttt
     6961 caacaatcaa atacaagtta tctgttaaat cgtctgtatt tacttgtata ggtatgtcta
     7021 tattcccttg tgaatcagtc ttcattctac ttacttcact gtattttttg tttagtaata
     7081 atcgtgcttt agcatcatca atatgttgac cttttgtagt atcactatcc gtccatccca
     7141 aggatttagc ttcatcttta gtaagtttca cataagagaa ctcaacgcct tcaagaggtt
     7201 ttacaccatt tggtaaagct atttctttcc ctttgttatc aatatctgag acagggttcc
     7261 catccccatt agtatttgtt actttatgga tatgaatcgt tccagttcct tgataattat
     7321 agttttgttg tgcatatgct acttgccccc ctagtccaag gctagaagag attgagcttg
     7381 cgcccaatcc cactcctagc aaagtaccaa atagcattaa atttgaaaaa attttttttg
     7441 atttcatacg tcagccttct tttttcatat ttacttcttt tccattaaca cttttttatt
     7501 ttgctttgtg ctgttctcga taatacagta gcaccatact caatcctgca attagaaata
     7561 caagaaggaa ggctgatgta ccgattccgc ctgttcttac tgtttcaggt acaaggtgat
     7621 taattactgt atattcaaag gtttgtccat cgtcagcaac ggtgatgcct tctttagacc
     7681 atttccctgt ataaccatta gggttttgtg tttcttcgat tttgtacgtt ttgttggcaa
     7741 caatattaaa tgttgccaag ccatttctgt ccgttttttg tacaactttt acacctttgt
     7801 cgtccgtcaa agtgaattct gcgtttgcta atgctttccc ttgatcatcg actttatgaa
     7861 ctttgatcac accttttttc aaagtgtttt tagctgtata ttcaaagact tgtccatcat
     7921 ctttgacggt aattccttct tgtttgaatg cgcctgtgta acctgttggt acttttgttt
     7981 cttctaagtt gtaagttttg ttcgcaataa tgttgaacgt agccagacca gttgcatctg
     8041 tctttttaac ttcttttaca cctttttcat ccgttaaggt gaattctgca tctgctaaag
     8101 gttttccatc ttgatcgact ttatgaactt ttactacacc ttttttcaaa ttatttgtag
     8161 ctgtatattc aaaagtttgc ccatcgtctt taacactgat accctcttgc ttaaatgcac
     8221 ctgtgtaacc tactggtact ccggtttctt ctagactata cgtcttgttt gctacaatat
     8281 tgaagcttgc aagtccattt tcatctgttt tttctacttg tgttgcacct ttttcatcgg
     8341 ttaaagtgaa ctctgcattt gccaaaggtt ttccatcttg atcgacttta tgaactttta
     8401 ctacaccttt tttcaaagtg tttgtagctg tatattcaaa agtttgtcca tcatctttaa
     8461 cggtaattcc ttcttgcttg aatgcgcctg tataaccagt tggtactccg gtctcttcaa
     8521 ggctgtaggt cttgtttgct acaatatcga agcttgcaag tccgttttca tctgtttttt
     8581 ctgcttgtgt ttgggatgaa tcatccgtca aggtaaattc agcattagct aatggttttc
     8641 catcttggtc aaccttttta acttttattt tacctttttt aattgtgttc gtagcagtgt
     8701 aactaaattc ttgtccatcg gtattaacag taataccatc ctttttaaat gaaccttgat
     8761 aaccttcagg aacttttgtt tcttctaagc tgtatgttaa gccttgttgc aaatcaaacg
     8821 tagctaaccc atcatctccg gttgtcttaa cagatttagc attgttattg tcagttagag
     8881 tgaactccgc tccttttaaa gcatttccat cttgatcaac tttcttaact gttacctgac
     8941 caacagctct tgtccattta gcactcaaag atttagagtc ctttgtttct gaagtagtga
     9001 tcataactac ggcatcttga tacacacctc gtccatcaaa agtaaaagga ttggtcgtgc
     9061 taacattagc agtaccttta aatgacaaag taccacttgg tgtagtcaca ggtactttaa
     9121 tagtaaaact tgaatttaat tttacagtgt tgttagtaat ttctttacca tctgcatcat
     9181 aaatcttcat accagcaggt tttgctgatg taaaagtgag cttcgcatta ccagtcttat
     9241 ttgatgctgt tttatatgtg aaagtgtgat attttccttc gtcagctgtc ttagaagctg
     9301 tgatagaaaa ctcagtgttg ggcgtatctt ttccattctt agcatagtca taaatttttt
     9361 caaatgcttt ttttacacga gctgtttctg cggcggtatg gtttggatgt gaccatacaa
     9421 tctgtgaaac tttaaagttt ccggctaaaa cccatgaaac taattgtgtt gcataccatg
     9481 cttctgtatc atttcctgca cccagttcct ttgctgtttt tgatggaaaa ccacctttaa
     9541 acatacgata gaactcatca ctgccagctc ccttgtactt caagcttccg gctccacttc
     9601 ctgttgggga tggcaattct gaatccatac aatatccaat atttccatga gaatctttaa
     9661 attgacttga aatcatccat tcatttggat gattcggcat attaacccta atataaccgt
     9721 ctgtttttac gccagtttct gtaaattttg cacttgtcgg ttttgtgaca tctgcttcag
     9781 ctcttattgc tgaaaaaggc attataagcg tagataacac cattactaaa gtcgcaaaac
     9841 aataaattat ttttgatttt aaaattatcg ttttttcatg cttcatctct cgttactcct
     9901 tctttatacc agttaggcga agaaaatgag ccacgatatg tcggtgtggt aatgaccttc
     9961 gcgcccattc ctaagttaaa atcaccacac ctattaatca cttttttcgt tgaaagcaag
    10021 atgtttcaac gctctattat tcatagcatc acccctttta gttctaccca ccatgagcaa
    10081 caagttttct aagtagttaa gtaagggcaa ataaagacta tacttaacta cttagtatat
    10141 aaacgatctc ttttattaac gtaccgaaac cgctaattga gatacgttat atgctgtttc
    10201 aaatgatcct gcattaatcc aacttgcccc aaacacagga tcgacaacat ggtaaaaacc
    10261 gtctttaaat ccatcaacgg ctactacatg tcgattccaa atagcatcgc cccaaaacat
    10321 tgatttatgt tgagcaggtt caaaggcata agttacccat gctactacag gatttccatt
    10381 tacaatctca tcttttaatc cttgtaccga tgaaccagaa atatctacaa catttccgcc
    10441 attagcaaca ccccatgaaa ctacaggagc cggattcata ccttgataag ttccatccac
    10501 attgtgtctc cattcaccag aaaaaccatt atttggatta ttatcaggag atagtggcat
    10561 tgatttaaca aaagtcatta aatcttgatt tgttgcaaca cctttataat gtaacgcttc
    10621 tagcaaagat gttccttcgc aaagcattgt gttaccttga ctaataaatg gaacgcctag
    10681 cacaacatta gttggttgtt cgggtttact tggtgaagta gaactgtcag tttcatcact
    10741 cgaacctgtt tgtgattgat cactagaaga tgtactggtt gattcatttg catcatttga
    10801 tgttgattgt gagttatttg ttgatgttcc agtatcagct tccgtagtgc tactttcact
    10861 tgttccctgt gatgcttctt tctcacttgt agaattattt gtattagatg aacttgctcc
    10921 atcaacactt gaagcacttg tttctgtctt atcagaagtg tttgctgttg gttccgattc
    10981 ttttgtagtc tcggattttt tgttctcttg ctccgtattt tcaacaggat caggtttatc
    11041 cttttgaaca gtttgactaa ctacttcgcc atctgcttcc ttaactgtta atgtatcttt
    11101 actgatgttg aaagttcgtc caattgggat agtccgttgt tctccttttg ccaaatcaac
    11161 attgttcaat tcggcaaact cagcaacagt agtcttgtaa cctaagcttt taagcttctc
    11221 tacagttgac caaatagtat ctcctttttt gaacgcataa cttgattgtc ctgttgtaat
    11281 ttggatttga tccggtgtgt ttgcagtcca ttcatcagca tgtgaggtta ctggtactga
    11341 taaccccgca agtacagctg atgcaaataa aaccatacca agcgaatttt tcttcatatt
    11401 cattccttct ttttgttttt attttctgtt tgtgatcgca atgcttctat tccttcttcc
    11461 gttataatat aggttttgtc acccactttc ttgaacgaac ctttaggcaa acgatttgga
    11521 taatagttaa ttatgtttgt aacataattt ctacctttac ccaaaatcaa acttgcttcc
    11581 cttgcggtat acaagttgta tttgtctagg ttcaatttct ttttgtttgc gatttagacc
    11641 tacccctttc gcaaaattta ttgtataata atagtaccat tgcactgtca taattgcaat
    11701 atttttttgg ggtttatctt catttaatag cctaaaaatg cttgttagat aaggaatatc
    11761 taactaatca aaacaaaaaa ggaagttaag aaaaatgaac aaaaaaagga aattatcagt
    11821 attaattaca cttatttcta tattgactat tcttagcggt atcttgtcgc tgaaaatatt
    11881 caatcaaata aacacaagct caaatgccaa agatggaata gtcgtagaaa atacctctaa
    11941 ttctaaagaa actggtacta ctacaaaaaa agacaaatct gctgagatca ctacgaaaaa
    12001 ttatttaata aagactgttt attttgatga acgaaaaaca gcaattctca aagatgagga
    12061 aggatcagaa ttaaagctac ctataacggt atccgataag ttcaatggaa cgttctcctt
    12121 acaaaccgct gaaggatcac ctgacaatct caaagttggg caaacaatca ttcttggtaa
    12181 aaacgacgaa ctgtattcat ttgtaaaata aaaaaatgga gcaaaggcta gtcaagccct
    12241 tgctccattt tccattcttc aagaggtgta gctatttcaa caccaaagtt taaatactgt
    12301 ggatgctgta gatacatgat atccattcta ttttcgataa actcttttaa cgttgagtag
    12361 atgtattttt ctaatgctat atctgcccaa ctaccataat caagtttatt cgtgatcttg
    12421 acgtttattt caatttgaga aacatcaata ttttgaacat caggaaactt attcagtctt
    12481 agctcttctc taaccgcttc tactagcggt tcatattgtc cgcctagttc gctaattgtt
    12541 gaaactattt catctgaatt ccaacgatct tccaatacct ttgctacatt ttctttaggc
    12601 ggttgtcttt ctataaattc ttcaggtgtt tcatcaaact ctactctaaa gttcaattta
    12661 ttttgattgt tagcatacat ataatcatct ctgtttgatt caaaaaagtt acgaattcct
    12721 gtgtctgatt tatcacttaa ctccttattt gctaaagtag cccaaaaatc ccaagttcct
    12781 ttattttcag tatacaaagt aaaattaatg tttatatagt ttgcagtttc aggataccct
    12841 tgctctctaa gagcatcttt aacaatcact gctaagtcag tttcaaaatt ctccataaaa
    12901 aattcactaa attcttttgg attccacgtt tcttctaata acttttctaa tttacgatct
    12961 gccattatta ttctccccca ctttactttc aatcttatat ctaaaactgc ttaaaattaa
    13021 gagctatacc ttatcataga aggtatttta tgctttattt aagtctacaa ttctatatta
    13081 caattatcat ctagaccatt ttcttatgtc aaataaacca tatatattgt ctttaatagt
    13141 tttatttgac tttttaagaa agtatttact tttgactttt tttacgtttt acaacatata
    13201 cgtattgtaa tgttcagctc aatttattat aatctaaatg aattgattaa taaaaattct
    13261 gaggttcggg ttttttatca attcttttct gtttgtgttt gtgatgaaac aatccttttt
    13321 cggtaggttt tgttatcatc gtcagcacaa agagctaacg ttggtaccgt tagctctttt
    13381 tttattttac tctgctctta catagctatc taaatcctcc aagtaaaaca attgattttc
    13441 tcctatgctt gttactccac gccaaattgt tgttgttgct gttgttggag tatttcttgt
    13501 ctgtgtaatc aattcaacca ttttaccttt ttgatttgtt gtatagtaat cagcagtata
    13561 gctagatatt ggactattac cagtgcctac catcgtgcca gaaatatcaa caggactaga
    13621 ctttactatc tgaggaaatt ctttttgtat ttccttcata ttcttcgtat atttttccca
    13681 tgaattccag ttgtaaaata aattaaagaa tttcctactt tgttccttga tttcagcatt
    13741 ccccaattct ttgtattggg cttcctgcat agctttcact ttctggttat tcttgactac
    13801 ttcgatttgt ttacttttaa ggtcttccaa ttctttgaca ttattatcat aagcattttt
    13861 cttttgaaat agtaccacac aacaaaggat cacaattatg gaaagtattc caatcgcctt
    13921 tttagcagta gtgatttcat gattttgcat aaaaagcacc cccagtcact ttcttatcat
    13981 tatttagtgt gatggttaat gtggtatcat acactttttt tccattgtat tgaacttcaa
    14041 aatcggtcaa tacttcatac aacccactac tagatgtctt aattccaaag tttgttagct
    14101 taaaagtaaa aggcttatag tactcgattt tttctttatc actactctta tcagtaattg
    14161 gtggtttacc tgtgacaata aatagttttc tcaaaaattc ttcacctgat tgatctgcaa
    14221 acttattttg aactgatcca ttcaacattt cttgaatatc attttctaaa tctttccgta
    14281 atccatccac ctcatttggt ttaacctctt taaagaatgt cagcttatct tcaatatgca
    14341 cactgtgttt ttcagcttct atgtcctgat tctgcttatt gaagaatagg attccaacaa
    14401 gcagtgctaa taacatacca gaaagcaaag aaatgataat tgtatctttc actttactat
    14461 tcatctatat cgtccttcat tccaatttat ttttcgattt caaatttagc ttttcctaca
    14521 ccgctaaaga aatgcacatt atctgatccg tccactccat caatcaaagt taccgaaact
    14581 ccccttgttg gagcttgggt tggtggaaca ttaaagttag cacatagaaa tgacccatct
    14641 tcaaacaccg ctataactac accagtatgc cctgcatcgc caacagccca taaccatcca
    14701 tccgaagcac taaacccata acctacgatt ggtttgttcg ttattttcgc tcctgccgat
    14761 ttgtagcttt cccatacata gccaccattt ccagtagtcg gttgattccc atcgtataat
    14821 tggctcatat aagcccacgt caactctgta cactgacctt gatatattcc gttaaaaggt
    14881 gaagttgacc aatctggttc tttgaatttt ggtagcgtga tcgctttccc atattttttc
    14941 aaagcttctt cataagtcat attcgctatt acttgacctt ttttaccgtt aatttttcct
    15001 tctttaccca ttgatccaga accagaatta gcatcatctg tagttgcaca ggcatcttct
    15061 actgttcctg atgctgagct tgatgaatta tttgatccac caccggtact tgttcctccg
    15121 gcaattttat tgattttgct tacatcttta gctccgtcca tgcctttgct tttcatgtat
    15181 tcccaaattt cttttgcatt tccaccacgt acagccacta cagctgattc ggaatctgct
    15241 gaaatttccc aattcttatg aaacgctacg gcatttgcca caacatcatc agaagcgttt
    15301 aaagcataat cttttaatat agcaacaaat ccaccatcgc ctttgaacat aaaatccaat
    15361 tgcgcttctg tcgtccacca agcgccacca aagtttttct tcgcccagtt tactaatgca
    15421 gtatggcgac ctgctgtcca ttgaccatag ccaataccca tcagaccatc agtgaattgt
    15481 ttgataaagt caacttctct ctgtccatca ccgtaataac cttctaccgt tcgaggatca
    15541 ttacctgatt cttgtttcca gttacctaga atacctgcga tagtagcacc tgatagccca
    15601 tattccccat tcatgaattt gtaaatatca tcaatatttt ttgctacatc acctgataca
    15661 gcaccactcc ctgtttcatt tccatcacta tcttttcctg atccactaga cccttgcgag
    15721 ccttcagtag aacagttgaa ctgtgcatca taagtagcta ttccatcagc atgtgtcact
    15781 tgcggattta tcgtgattaa actcgctgta atcccgatta tctgcatcac tgcgataagc
    15841 aacgtgccga acgttttctt cgattttttc aataactgcg cccccaaaag tgtacatttt
    15901 tatgttatta tgatattata ataacataaa taagtagaaa ggaaaagcta attaatgact
    15961 gataaaacag gtaatctacc taaactagct ccaaaaacat ctcaaacaaa gagcaaaaaa
    16021 gatgaaaaaa ttaaaaatgc tgtaaagaaa aaatcagtaa aggaatcatc ctcgttacta
    16081 cttgatattc caatgagtac ttttgatgaa atacttgatg acaatacgga tgaaacttct
    16141 gttaaaattg catctcacgt tggaatgaac agtcaaaatg attcttttta ccacttgtta
    16201 aacagcctaa gagagttgga agataaaaaa aatcaggtaa tcgcatcaaa agctcctgac
    16261 ctcacaaccg atttggatgc tgaaattatc caagtcaaac aagatgagcg atcagttaaa
    16321 tcgctactga aaacctattt tgatggtttt gataaagaac tagctccata tagccaagca
    16381 atcctaacta aaccaataca agctccacat atttcaattt ccgaacaaaa aaagagtgat
    16441 gactaaatag tcattactct tttttcaaat tttgtttatt aagga
//
LOCUS       DAZUKC010000056        15962 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000056 DAZUKC010000000
VERSION     DAZUKC010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.057"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(59..1369)
                     /locus_tag="ACG80T_001829"
     CDS             complement(59..1369)
                     /locus_tag="ACG80T_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFS4841465.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1381..2799)
                     /locus_tag="ACG80T_001830"
     CDS             complement(1381..2799)
                     /locus_tag="ACG80T_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4841466.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3039..4607)
                     /locus_tag="ACG80T_001831"
     CDS             complement(3039..4607)
                     /locus_tag="ACG80T_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFS4841467.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4611..4892)
                     /locus_tag="ACG80T_001832"
     CDS             complement(4611..4892)
                     /locus_tag="ACG80T_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFS4841468.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(4889..5812)
                     /locus_tag="ACG80T_001833"
     CDS             complement(4889..5812)
                     /locus_tag="ACG80T_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFS4841469.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5809..6687)
                     /locus_tag="ACG80T_001834"
     CDS             complement(5809..6687)
                     /locus_tag="ACG80T_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFS4841470.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6674..8011)
                     /locus_tag="ACG80T_001835"
     CDS             complement(6674..8011)
                     /locus_tag="ACG80T_001835"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFS4841471.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8014..9117)
                     /locus_tag="ACG80T_001836"
     CDS             complement(8014..9117)
                     /locus_tag="ACG80T_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS4841472.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9191..10699)
                     /locus_tag="ACG80T_001837"
     CDS             complement(9191..10699)
                     /locus_tag="ACG80T_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS4841473.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10665..11357)
                     /locus_tag="ACG80T_001838"
     CDS             complement(10665..11357)
                     /locus_tag="ACG80T_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFS4841474.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11379..12353)
                     /locus_tag="ACG80T_001839"
     CDS             complement(11379..12353)
                     /locus_tag="ACG80T_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4841475.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12653..13525
                     /locus_tag="ACG80T_001840"
     CDS             12653..13525
                     /locus_tag="ACG80T_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4841476.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14107..>15962)
                     /locus_tag="ACG80T_001841"
     CDS             complement(14107..>15962)
                     /locus_tag="ACG80T_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4841477.1"
                     /translation="SLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGI
                     EMVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQ
                     PTVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVA
                     VSLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKE
                     ALSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLE
                     DSHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEY
                     CRLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEI
                     VPAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVR
                     LINELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAIT
                     DHEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNH
                     RLEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
BASE COUNT         4680 a         3376 c         2609 g         5297 t
ORIGIN      
        1 cattaaaaaa aataaaggtc aagctaaaat attatttaag cttgacctta ttataatttc
       61 aacgattgtc aaaagtattg tttgcagctg tgttgccagt ttctatagat aaatcacagc
      121 ttttgaaaca taagttttgt actgcttcga ttcgcccgaa ttcttttgca gttatttggc
      181 aatgagaaaa gcagacatct tctataggct tttcaggaaa tgctactaaa tcaaaagctc
      241 gggaaggaag tgagtaacct ggagaaaggc atgcctgtac gtttttgatg tgtatgtctt
      301 tgactttcgt catttgcttt gattcaggta tttcttcagc cactgcttgc caactttctg
      361 gcatttgagc aaggtcattg ctattttttc tattgtattc attatgccag tccattatcc
      421 aagaaaaagg aaattgtaca tcaatcatat tcagattttc taccctgata ttttcgatca
      481 ctccccctct ttcttttgaa gatttcattc gaataccaca gtctgactga aaaaaattga
      541 tatcgtgaat atatacgtca ctaactccag cactaacttc gctaccaata gttacaccgt
      601 atcctgaacg gattttacat ctagcaacct cgattcttga tgattttcga ttgactcttg
      661 caccattgcc atctctgcca gacttaatag caatacaatc atcaccacag gccaaatcac
      721 attcatagac acgcacattt gttgaagaat caatatcaat cccatctgta cttggtccat
      781 cattgttgcg gatgattact ttttctacta atacatcgtt agaataagtg atttgcaagt
      841 tccaaaaacc tgatttctga aaaatcaaat cacgaacaac aacatgttct gcatgataca
      901 gtaaacaagc gcgtggacgt ttaatagcat aatccgcaat ccaacgaagt cccttttgat
      961 cgtattcagc tcttgtcccg cttttttgat cttttcccca atacagctcc caccaatggt
     1021 ctccttgacc gtcgattatt ccttttcctt ctataagaat atctttcgcc gagagaacat
     1081 tcaaaatcgc tgctggccat ttcatttcta cgccggctac tcgtgtatcg atttctggaa
     1141 atgcttcgat atctgttctc cccttgagga ctgcaccttc ttcaaagata agatgactct
     1201 tatcttttaa aaaaagggct ccgactaaat aaatgccttt tggaataaat acaggtaagt
     1261 gttctttcgc accttgatcg attgcttttt gcagtattat tgtatcatct tgttgagtgt
     1321 cgtttgtttt aaagttcaaa gcattgataa tccctttttt tgtccgcaat acgtttctcc
     1381 ctatctttca ttgatttttt tcaattcttc accaataaat acagtttttg aacgattcag
     1441 tgggtaacct actagcaaaa tgattgcgga tacaaataag cagaatgctg gtaacaagtt
     1501 tgccatcgta taaatccttt cttgtactaa aactgattgg atggctccac ctgttgtaga
     1561 cgattggtaa ccaatgagtg ccagcataaa tccgcctacc gctcctgcac atgcttgtcc
     1621 tacttttctc gcaaaagaat ttactccata tactgttcca tcttctctta agcctgtctt
     1681 atattgatga taatcaatca cgtcagtaat gaatgcccag accatcaaat tgaataaacc
     1741 agatcctaaa gtagcgataa ataaaagaat cagatatacc catgcatttg ttatatgaag
     1801 gaaatacatc agcaagtaca tacatgaaga agcaagtaac gcaaatacgc ttgcttcctt
     1861 tttaccaaat aattttgtca gctttgtagc aaatggtgct aaaactacta ctgttccgta
     1921 agtaaataaa agagcaacag acattgcttg tttattatgg aagtaatcat tgaataaata
     1981 agtcatattc gtcccagcta aaatctgatt gatcacaatg aaaatgtcaa caatgactaa
     2041 aataatcagt gctcgattag ctaacaaacc ttttaccaac tgagaagcag atactttttc
     2101 gtttttgaca actcttactc tttcagtagt caatttacaa gttaacatat atgcgacaaa
     2161 tgctattgca gcacatccga ttgaaatcca aaagaaatga ttccctgaaa cgacttgttg
     2221 tccactcttg ttatgggtat acattaaaat cggaatgaag aatcctgtag ttgatccgcc
     2281 aatcgcagaa ccaatgcttc ggaacgtgga caacgaggtg cggtcattag gatttgagct
     2341 aattgctgct gccattgctc catacgggat attgattgtt gagagaaaaa tcccataaac
     2401 aatgtatgaa acaaaaatat aagcaatctt caccgtcatt ggaagatgtt ggacgaatgg
     2461 caaaaacaaa atgacagtca ctaagcaaaa tggatacttc atttttctga tccaaggaat
     2521 aaatcggcca gtttctgtta actctgagac atcacataat cttccaacca tcacatcagc
     2581 aaacgcatcc aaaattctcg aaatcaagaa taacgtacct acgattgccc ctgaaacgcc
     2641 aagaacattc gtataataaa tcatcaagaa actattgact aaacctaaaa taaaacagtt
     2701 acctagatca ccgaacatgt agcctatctt atcggaaatc ccaaattcct ttttttcttt
     2761 ttttactgta tttgctgtaa cagcattttg tgaaagcatt tgaattcctc ctacctcttc
     2821 tagtaaacgt tttcaaaatt ataactcttt ttttcttgtt tttatattca caatgttggc
     2881 tttttattgc attatcttga cagataaaaa acaaaaaata atctaaaaac attgatgcat
     2941 aagattgata acttttttta cataacaata tatacaaaca aaaaaattca ctaactagtg
     3001 aaaataagtt agtgaacctc atttattaaa gaattaaatt acagatgatc ttttccatca
     3061 tacttgaaaa actcaaaatc agcataactt cctctttttt tatttagatc atgcactgct
     3121 atcccgataa aatttccagt aaaccctcct gagagtatac acatgttttt ttgcggacct
     3181 actttcttcc attcttcagt tgatacgtct ttcaaatgaa actgtgctgt ctctttcttt
     3241 acagtgactt tcaaaaaagt cttcttttct gtgagtggaa tcgtccaatc ttctatttgt
     3301 gctactccgt tttctgattc tatcatacgt aggcattttc ccttgtcctc attccaagta
     3361 acataacaat aaatataatt ggattcattt aagtacagta ccagtccagc catctgatta
     3421 aaattattag gcatgtattc aaatgctgtc tccgcatcaa aagtaaaatc cgtttgccga
     3481 atagctaaca aatgatgttc gaaggtagat tgaagagatt caccggaaat cagacgtaaa
     3541 aaacctggtc tacattttgt atcacaccaa tcagcatttg gcaaaatgcg tctggcattc
     3601 cagttcttct ttagctctcc ttcaaatgta tccataaact gatgatcttt ttcttgttgt
     3661 acctcttcat ttgtctcaat cattatttct tccatcggag ttgtctggct atctttaagt
     3721 cttaaccaat cgtcggacgt ccaatacact tcttgaatag cggtttctct tcccaaaata
     3781 gccgcaccgt ttaatggtct ggtacaaaga tatgtcatgt accatcttcc tgacggggtt
     3841 tgtactaagc tcccatgtcc tgtacattgc aatgtagaag caggtttgtc actagctgtc
     3901 agcataggaa aaccaggatc aagctcgtaa ggaccagtaa tattctttgc tcggcaaact
     3961 gtaactgaat gtcctcttcc tgtaccgcct tctgccgtca tcaaataata atacccgtta
     4021 cgaaaataca aatgtggtgc ctccgttttc gctaattctg tacctgcaaa tattttatag
     4081 atgggaccat caagcttctt cttctctgca tcatattctt gtaatacgat accgctggat
     4141 ttattcggtg tgtccagacg ataatcccat agttcattga caagccattt ttttcccgtt
     4201 tcaagatcat ggaacaaaga aggatcaaag ccgttactat ttaaataaat tggttcggac
     4261 caaggacctg tgatctcaga tgcagtgatc aaataattgt ggacatcttt aaacggacgg
     4321 cctgcacttt tgacatctgt gtaaacaaga taaaacaatc catcagcata actgatctgt
     4381 ggcgcccata tacttccatt tgtaggattt ccttgcaaag atacttgagt ggtcaaaata
     4441 tccgttgtat gtgaccagtt gactaaattt ttcgactcat atactcggat tcctggaagc
     4501 cattcaaaag aggagacaat aatgtaatac cgctctttca ctcttaatat attaggatct
     4561 ggattgaatc ctcttaaaat aggatttttc actttgatag tctgcatgat tcaggctcct
     4621 tttctcattc ttactcgata taaactgaat gccccagctg ctaaaaaagc aagtgaaata
     4681 aacaacacaa cggcaaacag accagtcatg tcctgtaata atccaaatgt attttctaaa
     4741 gacaaagttg aatgatctac agcaagcgac tgataacttt ttttaaactc tgacgcagac
     4801 atattactta aagtaactgc taacccgcca aagaaaatca aagatagact gccaccgacc
     4861 attcctagca atagttccca ttttcttttc atttctgaac cccctgcttt tctaaatgta
     4921 tccattcagg tcttttgatc gatgtttcat aattgttatt acgttcagag aatttgacag
     4981 tctgttcatt tttcttgtca tcccaattat cccaaccagt tgggtgaaca tgcccaccta
     5041 tcttacacgc ttcaaataag acatttgcat acggacgcca cggtcttcct aaatagacgt
     5101 ttctagtatc aggttctgca gtcaaggtac agctttcaaa acgataccct gttttttgat
     5161 ttttcggcgt agatgctgct gtgatgtatc caggattttt actggctaag ctatggatat
     5221 gacactgatg aaaaacggca tctgccccac caaatatgaa atccaccgtt ccttcaatcc
     5281 agcaatattc ataggtctga cgacaatact caggattggc agaagccggt gtacaaaaag
     5341 gagtaccatc tttttgcaaa tcaggcaacg gacccgtgca taaggtatcc tgataaccag
     5401 atagtcgaca gttttttaaa gatacgcgat ctcctgaatt gaataaagca acagcctgtc
     5461 ctaccttttc tcctggacct gctgtatttt cgatgtgaag attttctaat acgacattcg
     5521 atccttctaa gaaaaaagta gcacttcgga aagtccctaa ctggctaccg tcaagatact
     5581 cctgttttgc aaaacgatta ccggagataa ctactgttcc tgctcccaca agtttaaaat
     5641 tggataatct gctttctata tattcatcat atcttccttc ttttactatc aagacctttg
     5701 gatggctatc cggcaggcct gccaaataat cgattccttc ttggatagtt tcaaaatccg
     5761 tcggggtatc tatagcattc tttccaatta tcagtttgtc tttcatcctc ataaatgctc
     5821 ccttatgaat tgtttccaaa tatttctcat ttcttgtgtt tccatatgac cgcctgttaa
     5881 agaaaaagaa gaaaagtttt ctggactacc cattgattga tactttttgt ccagcccttt
     5941 tctcagcttc agtacgcctt cgtccaagca catcgtatcg ttttttccaa ctaagctaag
     6001 acgagggcgc ggagcgattt tttcttggat tgctaacgtt gtataagctg tcaatagatt
     6061 tggcacatag taataaaacc catgatgatc taggcggcga tgtagcaaaa gtgtttcgat
     6121 gtccacctgt gccgctagat caatacaaac tttgacacga gaatccaatg ctgtcaccca
     6181 ccagcttaat aagcctccca tagaaagacc aatcgttgcg agccgttgag tatctgtgtc
     6241 ttcccttgtc tccaaataat cgatcaagct gatcacatca taaatacgca ttccccataa
     6301 cgtttttcct gttaataaaa attgtttgaa caactcgctt tcagaaatac ctccgcgttc
     6361 ttcaaatccc caagcatcaa tcgaccaaac agcatatccc attcctgtca gtacttctgc
     6421 aaaagatgga gaagctaagt aaggagcacc atggatcaat tcacttttcc cgactgaaaa
     6481 atctccgccg tgagaatgca ggtaaaggac cgtcggaaac ggtccttctt tttctggata
     6541 agcatagagg gctggtactg tttccaattc atttaagtac aattgatagt attcaagcat
     6601 aaaaccgttt tgtttttctc ggtataatcg ttttccacta ggggtatgtc tgctagatgg
     6661 agcacctaaa aaatcattca actgcataag caaagttttt acgaacaacc agttcatcca
     6721 tattttctga ttgatttgat tggaatttct caatactgat ttctttagta ttttctaata
     6781 gaaatgctgg tccctcgtga ttttcaatcg acatctggtt aaaggaaacc ttacccgcaa
     6841 agccaatata aaatccttgt ctgctcattt cctctattcc tgtcatcata gcaggaacac
     6901 cgggaactgc atcttgggcc atcgagacat cgatattgtc aaatgtgatt tcttgtatga
     6961 attgttccgc taatccataa ataaaacctg cagaagcatg gacattcctt gccgtgatat
     7021 tcgaaaaatg gatccttctg aatgctgatg tccgttcatc tatcggataa gccttttttt
     7081 cccatacata cggttctttc cctcgcggac cacagaaata ataaagattc agtataaatg
     7141 gacacatcac attatccatg actatattgg atactcgaat atcttcaacg atcccgcctc
     7201 gacctcgtct agatttcaat cgtattcctc gatcagtttc ttggaagata caatttgaaa
     7261 tggtaatatt acgaatacta ccactcattt cacttcctaa tacgacaccg ccatgtccat
     7321 ggaccatcgt gcaatttgtg atcgtgatat tttcgcaagc gatcctttcg tacgtatctt
     7381 cagtccctgc cttgattgca atacagtcat ctcccacgtc aatatggcaa ttactaatac
     7441 gcacattttt gcatgattct ggatcaattc cgtctgtatt tggtgaatcc gctgggttca
     7501 agatcgtcaa gttatcaaac gttgcattac tgcaaagaat cgggttgatc gtccaacttg
     7561 gtgactggat caatttaata tctttcacag taattcgatg acagttatgg aaactcatca
     7621 atttaggacg aggataagcg agattatctg gttcatttct aaaagtatgc caccattttt
     7681 ttccatttcc atctaatgta ccgaaaccag tgacagagat gttctctaca ttttgagcat
     7741 agatacagga agcataaact tttcgatgga ctccttccca acgtgaatga acaacaggat
     7801 aatcttttgg atcgtcagag aatttcagaa ccgctcctgc gcttaaatga agttcgacat
     7861 tgctttttaa aaataaagct cctgttagaa attcacctgc tggaacaact acggtcccgc
     7921 ctccgtcact tgctgcagca tcaatggctt gttgtattgc ttcagtattc aattcatcaa
     7981 ttgatgcgcc aaatgtcaaa atatcataca tatttataat gcctccactt caatcaatgc
     8041 ttgcaagaat gcaccaacgc ctttttgatc attacaaata attggttcac tgatgtagta
     8101 agcatatgaa ccatctcgct gatctgctcc gcctaaccct gctacttgac agtttttatt
     8161 caagttcagc cagccttctt ttgtttccag aacaaattca tcaagtaatc cttgatgtga
     8221 tttcttcact tgtggaagcc attcttcttt gttcagcagt cccaaacgta ttcctttagc
     8281 cattgcgtaa acaaacatac tactgccgga agcttccaga taatttcctt tccgttttgt
     8341 ttctgttgtt acttgatacc atgtcccgct gttttcttct tgatatggaa gtaatgcagc
     8401 cagtgtcttt tctagaattt ctttcagcaa agaacaatct gtttcttcat gaagtaattc
     8461 ataggtatct gctgcagcca tcaaatacca gccaatcgat cggctccaaa aatgatggga
     8521 tagacctgtt tttggatcag cccatggttg gaccttcttt tcatcccatg catgaatcag
     8581 caaatttgtt tttggatcga atagatgttt ataactgatt tcaaactgtc tcaaaacatc
     8641 ttggtaatct ttttcttcac taaaattctg gataaattca gcataaaaag gagaacccat
     8701 atataagcca tccaaccaaa tttgataagg ataaatttct ttatgccaaa aagctccttc
     8761 ggatgtgcgt ggatggtttg ccaactgctg ggaaaggagt tctgctgctt ttttatattt
     8821 ttcttcgcct gtttctttgt acaataaaaa gaaaagcttt cctgtattta catggtcgat
     8881 attgtattct tcaacgctgt agccacggat cgttccatct tcttgcacga aatagtcgat
     8941 attgtctttt atgaaatcaa aataaatttt ttcttgggtt cgttcccaaa gtaattggaa
     9001 ccctttaagt acgacgccat agtcataaga ccattttcct ttataccact tttcttcata
     9061 taagcgaggt gttctttcca tgatactttt tgctaatttc tttgaccagt tttccattat
     9121 ttgctctcct ttaaaaatta aaataggatt tgggacaaat accaagaagt atttgtccca
     9181 atcgtctata ttactgtgcc gctgctttgt atgcttcctc gtactcttgt gtgattcgat
     9241 cgcctccttg tgctttccag tcttctacag cttttttgaa tccatcttcg tcaagctgtc
     9301 ccatgatgta ttgcaccgta gcatcaatga cgattttttc aatttctgat cctacttcat
     9361 tgttcgtcgg tgaatccaat ggcacagaag gatccattac agcaaattct tcattttctt
     9421 tgatcaattg attagagaat tctttttcag ggtctgtatc tttgaaagag aacgtcactt
     9481 cactaggacg agagcttgat aacggttgca cctcttgttg ccaaagatcc atatcgataa
     9541 attcgataga accatcatca ttcaattcat aatgcgttcc ttcgataccg ccagtcatca
     9601 atttatacac atcttcttct aaaagatcat caacaaattg aagtaatttc ttcaattgtg
     9661 cttcgtcttt tacttgagat ttgggaaatg ctagcaagcc gccaatacca tttccttctg
     9721 accaaacatg ataaccgccg ccttcaccat ctgagatctt gtttacaggc actaattcca
     9781 tatcatcttg aataccttga cccatcgtct tcagattctt gatatccacc attccagtgt
     9841 agattccagt tcttccttgt gcaaattgtt cttgctgatc agtcttttgg gtaactgcaa
     9901 agtcttgtgc caaatagcca ttctcaaata gatctcttga aaaattcaaa gctttcatat
     9961 actcttctgt atcaaattct ggagtgaatt tgccattatc gtctacagcc cagccatttg
    10021 gtgctccaaa ataagaagtg aaaagtttaa agctggtata acgaatatca ctaggcgcac
    10081 gatcgccaaa accaactgtg tcgtccacac catttccatc aggatcgttt tctgtaaatt
    10141 gtttagctac ttcataaagc tcatccatgg tagttggaac atctagtcct agattatcca
    10201 accagtcttt tcgaatgact aagccagcac gagcgtagtc tttttgtatc ggtacgccgt
    10261 acaaggcacc gtcgatactc gcagcttcga ttctatcttc agaaatttga cttaaatttt
    10321 tatagtcttt taaatattct gatacatccc agaatacgcc tgattttaac gcattgcgga
    10381 cagaagagtt tttcaacatc gcaatttgaa gtgtaacgat atctcctagt tcatcagaag
    10441 ccaatgcagt tgttaatctt tcttcttttg aagcatcagg aatccagttg aatgtcagtt
    10501 ccgtatcggt tttttcttcg atcaagttca caatcgtgtc tgtaggcggt gaagcatgat
    10561 gcaaaatatt taaccaagtg attttagctt ttccatcttc tgtctctgaa gaactgttac
    10621 ttgaactgct gccgcatgcg gcaagtgcta gaattcctgc aaaactaaga cctaatttta
    10681 cgagcgttgc ttttttcata aattttctcc ttcactgttt taataatatt tttgttttct
    10741 tctgtttttt ctgtatatga gttcttccat tccaaatggc aaaatataga atttgctgtc
    10801 cacgtgactg cggaaaagaa gagactgcca ccgataaagg ttaaaacacc tggcattgtt
    10861 tggatgatca tcagataaat ccctaccata cttaatatca acactaatga gtaatgaaga
    10921 tgtgagaatc cgaataaaaa tgccattttc attttgaaaa aatatccttt ccaatcataa
    10981 tgagccatga ttgggaaaat atagagtgct gagagcacat atatgaacgc agcaatatac
    11041 aaagcgatcc taagatacca attgtgtatg aaagaaagat ccacaagtaa aacaatcccg
    11101 atggcgccat atatccagct catcagcaag gtttcacgat agttttcttt aaaatatgtg
    11161 aagtacgttt tcgtcaaagg aaacgtttct tttgagcgca gccattttct aatcactgca
    11221 accatcgcat acgatgcagg accgaaagta aaaactccca aacctgcgat gactaaaccg
    11281 ccccacagaa aattcaaata aaccaactgt aaaatacgat ttagccttcc gtttatttta
    11341 tctaatgact gcagcatatc tttccctctc tttctatctt agtaaacgcc gtcttctcct
    11401 agctttttgg caatcttatt agctaaaatt accataaaca atcctacgac ccctttaaac
    11461 aatcccattg ctgtactgaa acttaactgt ccattcttca atccagctgt ataaatatag
    11521 gtgtcgaaaa tttctgcaac gcttcgattc aatgaattca ataacaagta catatgctcg
    11581 aaccctaagt ctaatgtctg accgattttt agaatcagca aggtaatgat tactggacga
    11641 attgccggca atgtaacatg ccatgttttg cgtaagcgtc ctgctccatc catttctgca
    11701 gcttcataca actgtgtatc gactgcagtt atcgctgaaa ggtagatgat cgtagaccag
    11761 cccaactctt tccagatcac ttgtccgata tatactgtgc gtgtccattc aggggatgtt
    11821 agaaaactga ttttttcaaa tcccattgtt gcaaggaatt cattcaatac ccctccgtca
    11881 acattcaaaa atacataagt aatcgaaaca atgattaccc aagacatgaa gtgagggatg
    11941 taaatgatcg tttgaatccc gtttttcaat cgtttgttcg ttacttcatt taatagtaaa
    12001 gacaaaatga ttggcatcgg aaaaaacacg aagatattta atccgaacaa gatcaacgta
    12061 tttcttaata atgtaaagaa tgtcggttct gtaaacaaac gaatgaaatg tttgaaaccg
    12121 acccaaggac ttcccattat tcctaaaaat ggttgataat cttgaaaagc aataacgagt
    12181 ccccccatag gaagatacct aaaaatcaag aaataaagga ctcccggcag aatcatcaca
    12241 tataaaaatt tattcgtttt gattcctgca attatttttt ttcgtcttct gagcttttct
    12301 tcttttttcg atagcgtcag ttgaaatggt tcttgtttct ttacactttc cataagcccc
    12361 tccttagttt tgtgaatgtt tgctgcttat gacttcatga tagataacta tctgaattac
    12421 gtatataatc atttttatcg taagtaaaaa cgcttacata gcaatgattt ttgtgctata
    12481 caaaacgata aagtgtattt tttcacaaat aaactacgca tagcaaacct tctatttttt
    12541 tctatctgac acttagcata ggaaatgatt atatacgtaa tctttcaccg atgataaatt
    12601 gaacatatag aaacagaaac atttaattta ttttgaggag gagcctatct atatgaaaaa
    12661 gaaagtctct ataggagaaa gaagttttac acttttcaac aatacgtttc taattttgct
    12721 agctttgatc tgtatcgttc cttttttaaa tatcatcgct acatcttttg cttctacaca
    12781 agaagtcgtc gcgaaaaagt ttatcctttt tcctactacc ttttctttag acgcgtatcg
    12841 ctacattctt tctacgccga ccatttttag agcacttgct gtttcgattg gtgttacagg
    12901 tgtcggaacg atcgtcagta tgtgtgcaac ttcactgatg gcctacggtc tttccagaaa
    12961 gtatttgttt ggtcgaggtt tcgtcaactt tcttgttgta ttttctatgt tgttcagcgg
    13021 ggggatgatt ccaacgttct tagttgttcg ttctctaggg ctggtcaatt cttactgggc
    13081 catgatcctg cctgtggctg ttaatgcaat gaatatgatc attatgagaa actttttcca
    13141 agccttgccg gatagtttgg aagaatcagc aaaaatggat ggttgcacag attttggtgt
    13201 atttttcaaa attatgctgc cattagcttt accgtctatt gctactatct cgctttttta
    13261 tgctgttact tactggaaca cttatatgac tgctattttg tatatcaacg attcatctaa
    13321 atggccgata cagattcttt tacgccaaat cgtcattgta tcaagtggga tgcaagcaga
    13381 aagtagtgcc gttgatgtga ttccaccagc acaaacagtt aaaatggctg ttatcgttat
    13441 tgcaacagtg cctatgctga ttgtttatcc ttttgttcag aaatattttg tcaaaggagc
    13501 actcgtaggt tctgtcaaag ggtgattatg tactgaacgc aaaatggtag ttgtgtcaga
    13561 agtggtcagc ttcaagaaat aagaaggcat ttacgaaaat tgcttcttaa atttttgtga
    13621 aatttcggct tatttttcga agaatctact cctggaacac cgtgtatcag aggttgtgaa
    13681 agagacgttc agccttgagc gatgataaaa actctcacaa acattgcgac gagtaaccca
    13741 caggagcaat aagaagcgat tttgaaaaat agcttcccat atttcacaaa attatgcctt
    13801 attgtcgata gcctgtctct tgaacgccat ttattcgtaa aaaagaactg tgacaagact
    13861 tttgtcacag ttctttttgc gtgcttagcc ttgaaattta actatccata aatacaaata
    13921 ctagggttat tctaacttct tttattgctt gaatcagtcc acaagaaaaa cagcctcttg
    13981 attaaacggc gtttatcttg ttgattctgc ggcaataaaa tcagccagca gcaaaacatg
    14041 aagtgctgtt ttttgctgtc ctcttttatt gcttgaatca gatcacaaga aaaacagcct
    14101 cttgattcac aaatattttt ttcgatattc tcccggcgta atcgattcct tctttttaaa
    14161 gaaacgaata aaattttgcg gatttcgata ttgcaatctt tctgcaatct cttttacgga
    14221 gaggttagtc tctttcaacc atttttttgc tacctctaaa cgatgatttt gaacaaaatc
    14281 accaaatttt tctcctgttt cctttttaaa tacattactt aaatagttag ggttataatg
    14341 caacctatca gcgatcacat ctaaagacag atcacgatca tattctgtgt agacgatatg
    14401 cataatattc tccgacaaat ttttaaattc atgatcagtg attgcttgag agcattctgt
    14461 gattggcaga actaattgtt ctaaaacaag tttttctagt tctttgggat gataattgtt
    14521 cagtgctttt acataaattt gtttcatgtt atcaaagttt tttgaatcta ctcccaataa
    14581 ctgtcctaat tgaacgagtt cattgatcaa gcggaccgaa actacttccc gactcaaagg
    14641 atttttattt tgtgcaaaca atgcatcaat caattcatgt actaaattct tcacttcctg
    14701 atttccagaa cgaatggctt caaacaatcg attttgttct tctatcggat atctaataag
    14761 tgttttttca tgagcagcag gaacgatttc ctgataaaaa ataattgaac ttggaccagt
    14821 atttacacga taatacaggg cttcttttgc ccgatcaacc gattgcttgc tttcattcag
    14881 cgtttcgaac cggttactga tgcctatact gatagttaaa ttcaagtatt cttttacacg
    14941 tttttgaatg agtcggcaat actccatgac ttttttgctt gcttctgcat catctttatc
    15001 cattcgataa atagtggctt gcatttcttc attcaaaacg atcggcagca ttcgattttc
    15061 ctttggcaca atttccgtga tcatgttgtt gatcgctaaa agtagcaaat cacgctcccc
    15121 accgtgacta tcttctaaca gatcgatttg aaccaaagct gtataatagc agtcattttg
    15181 agaactataa ccaaattgct gcaaccgctg atctagttct cgcctttcta ctcgatttct
    15241 aaataaacta agtacaaata gcgtttccag ctgtggcttt tgggtaacta agtgtgcact
    15301 taacgcttct ttttctccta tgatccgact gactgattga gcgaccaaat ctagttcttt
    15361 cccactgaat ccttttgatt taagattcag acgttcctgt atttgagaaa tcggacgggc
    15421 aaatctgtcc gaaaataagt aggaaaaaag accaatgagt cctaccagtg ttaaagacac
    15481 tgcaactagc ccgattctca gattgcggat cattgcgcca attgcactag agtcaacttc
    15541 taatctgtaa atccaattat tataatctga tttcagtact atatacgttt tatctttttc
    15601 tcttatcact ttcagctgat tgtaatctag atccacgtcc gttaatacag ttggttgctc
    15661 tttatttttt actgtctgag tagtaaaaag gttgccaact tcgttttcaa tagaaagtat
    15721 gtcttctcca ctttcaccaa caacttgttc tattgtatgt ttctctatcg tagcaattcc
    15781 caatgcatat ttttcttttt caaacattgg caaagaaatc accatttcga ttcctttgtt
    15841 tttcttttgc caaaataaat tatttttgtt atttatgtac ttctgttgat acttttcaac
    15901 ttcctcttcg gtcagttgac tcaacgatcc attgattact ccccatttac cagccaaact
    15961 ga
//
LOCUS       DAZUKC010000057        15821 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000057 DAZUKC010000000
VERSION     DAZUKC010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.058"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..401
                     /locus_tag="ACG80T_001842"
     CDS             <1..401
                     /locus_tag="ACG80T_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptidase"
                     /protein_id="HFS4841478.1"
                     /translation="DKEMSPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRR
                     VIAVENNHEDFYTKADTKTYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLF
                     FTIAFCLTGITAWNTFVELKKKRRRTQKFL"
     gene            529..1212
                     /locus_tag="ACG80T_001843"
     CDS             529..1212
                     /locus_tag="ACG80T_001843"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFS4841479.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1521..1594
                     /locus_tag="ACG80T_001844"
     tRNA            1521..1594
                     /locus_tag="ACG80T_001844"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1555..1557,aa:Arg,seq:tct)
     gene            1753..2112
                     /locus_tag="ACG80T_001845"
     CDS             1753..2112
                     /locus_tag="ACG80T_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFS4841480.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2235..3866
                     /locus_tag="ACG80T_001846"
     CDS             2235..3866
                     /locus_tag="ACG80T_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFS4841481.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(3928..4569)
                     /locus_tag="ACG80T_001847"
     CDS             complement(3928..4569)
                     /locus_tag="ACG80T_001847"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFS4841482.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4755..4831
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            4851..5159
                     /gene="rplU"
                     /locus_tag="ACG80T_001848"
     CDS             4851..5159
                     /gene="rplU"
                     /locus_tag="ACG80T_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFS4841483.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5186..5530
                     /locus_tag="ACG80T_001849"
     CDS             5186..5530
                     /locus_tag="ACG80T_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFS4841484.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5543..5836
                     /gene="rpmA"
                     /locus_tag="ACG80T_001850"
     CDS             5543..5836
                     /gene="rpmA"
                     /locus_tag="ACG80T_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFS4841485.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6270..9077
                     /locus_tag="ACG80T_001851"
     CDS             6270..9077
                     /locus_tag="ACG80T_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4841486.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9268..9759
                     /locus_tag="ACG80T_001852"
     CDS             9268..9759
                     /locus_tag="ACG80T_001852"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFS4841487.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            9862..10851
                     /locus_tag="ACG80T_001853"
     CDS             9862..10851
                     /locus_tag="ACG80T_001853"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFS4841488.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            10884..11687
                     /locus_tag="ACG80T_001854"
     CDS             10884..11687
                     /locus_tag="ACG80T_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFS4841489.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11708..12622
                     /locus_tag="ACG80T_001855"
     CDS             11708..12622
                     /locus_tag="ACG80T_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4841490.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12705..13079
                     /locus_tag="ACG80T_001856"
     CDS             12705..13079
                     /locus_tag="ACG80T_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFS4841491.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13218..13463
                     /locus_tag="ACG80T_001857"
     CDS             13218..13463
                     /locus_tag="ACG80T_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS4841492.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13635..14699
                     /locus_tag="ACG80T_001858"
     CDS             13635..14699
                     /locus_tag="ACG80T_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFS4841493.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            14945..15370
                     /locus_tag="ACG80T_001859"
     CDS             14945..15370
                     /locus_tag="ACG80T_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS4841494.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15363..15812
                     /gene="nusB"
                     /locus_tag="ACG80T_001860"
     CDS             15363..15812
                     /gene="nusB"
                     /locus_tag="ACG80T_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFS4841495.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
BASE COUNT         5065 a         2622 c         3467 g         4667 t
ORIGIN      
        1 aggacaaaga aatgagtcca tattatgcac aaggaaatct gatatatgta cgaccgaaaa
       61 atacaaatga gattttagtc ggagatatta ttacatatta tgaaaattca ggaaaaaaaa
      121 tctcgacgcg tcgcgtaata gccgttgaaa acaaccacga agatttttat acaaaagctg
      181 atacaaagac ttatattgag cctggagtag taaaaaagag aaatattatc ggtagtccca
      241 tctttcagat tccatatatt ggactagtcc tttcaaaaac ggcatttgca tggattcagg
      301 ggttattctt tacgattgct ttttgcctaa caggaatcac agcatggaat acctttgtag
      361 agttgaaaaa gaagcggaga cgaacacaga aatttttgta agaatgttaa aaacataaat
      421 gtattccaga gatccaacga ctcttctgaa catgaaacac gagaaaacac tcatgcagct
      481 tgtagattta gtcagttgtt cgtataatgt gtgaagagga ggagtagtat gcgcaatgaa
      541 atgatgcatc gtcctgtagt caaagaagaa ctattggatt ttatgcgtac ccagcaaaaa
      601 aaattaccgg gtgagctagg caaactggaa gaagaagctc atgtagcaga agtaccagtt
      661 attccccatg aaacagttgt atttttgaaa tttttacttg gacagcttca gcctgaacga
      721 atcttggaga tcggagcagc tatcggtttt tcatcaagtg tcatggccac gacaatcgga
      781 gaaaacgctc atgttacgac aattgatcgt ttcgatgtca tgatcgagaa agctaaaaaa
      841 aactatgaac gtctaggact gacagataaa gtgactttgc ttgaaggtca agcagcagat
      901 atcttgccgg agttgtctgg tccgtatgac tttattttta tggatagtgc aaaatcaaaa
      961 tatattgaat ttttacctga atgcttacgt ttgttaagaa aaggtggcgt tttgatggta
     1021 gatgacatct tccaaggagg aacgatcttg ctcccagatg aagagattcc ccgtggcaaa
     1081 cgagctatcc atcgtaaatt aaatgaattt cttcgtgttg tgatggatca tcccgatttg
     1141 acatcgacaa tcttgccatt aggtgatggt gtgatcttaa tgacaaagga atcagaaaca
     1201 atcgagttgt gatacatgat tatggcagcc tgtagataat cgattgaatc gagggctcaa
     1261 ttcgaacttg atgtaaaagt ttctatattt gtaagtcaat taaaggatac aggaaatcta
     1321 aataaacagg agtagcatga agtcaatgta gctcctgttt tcaattgata accttagaac
     1381 agctgaagga gaatgtctcg ttttaaaaga ttgatttagt agttgggcca aaataattca
     1441 ttatttgcta attaaataac tatttcgtat tgacagaagc aatttaatta tctataatta
     1501 ttgatgttcc gctgattctt gtcatagtag ctcagctgga tagagcatcc gccttctaag
     1561 cggacggtcg ggggttcgaa tccctcctgt gacgctaaaa aaccgttaga aactgaggtt
     1621 tctaacggtt tttctttgtt ttttgtgcca atattgtttg ttttcaaagg caaataaaat
     1681 aaaaaaacat ccttatcgag tacaattata aggagcaaaa aaataaaact catcagaaaa
     1741 gaggaagaag atatgacgat actatctaga attttagtga ctctagttgc aattgaattt
     1801 ttttatatca tgtatatcga aacggtgaga actgattctg acacgacaag tagggttttt
     1861 aaaatgagta aagaagagtt aagcagaaaa tctgttcaaa cattgtttaa gaaccaagga
     1921 gtgtacaatg gactattagg agtgggacta ctttatggag cttatctttc ttccgcatct
     1981 aaagaaatca caagtatgct attaatatct attttttttg ttgcattata tggcagtttg
     2041 actagcgatc gttggatttt actcaaacaa ggtgggctag ctctgttggc attgctttct
     2101 attttcttat aaatatagaa aaaataagaa tttatctaac aggaaacttt gtgaaaaata
     2161 ttactttatc tcatgaaata tgatattcta atgaatggta ttttagaaaa tttcatgaaa
     2221 aaggagttag ataggtgttc aaaaattatg tttcctcact aaaaaaagaa ttttcaggtt
     2281 atgatctttc aagatttcag aaagatcttt tggctgggat cacagtagct gctgtagcat
     2341 tgcctttagc cttggctttt ggggtatcaa gcggagctga tgcggccgca ggtttgatta
     2401 cggctatcat tgccggtatc gttattggcg gtttatcagg cggtttttat caaatatcag
     2461 gtccgacagg tgcgatggca gctatcttga tgtctattgc ggcaacaaag ggaatgcaag
     2521 gtgtattgct tgctactttt ttagcgggtc tatttttact tgcagcagga cttttccgat
     2581 taggaacact gacttctttt atcccagcac ccgtaattac ggggtttact tcaggtattg
     2641 ctatcattat tgcattaggt cagatcgata atttatttgg tgttcactcg gaaggtacaa
     2701 acgttattga aaaactaagt agttatcaaa cgataggatt tccgataaac acagcctctt
     2761 tgatcatggg agggctagtc attttaggga tgtttatcta tccaaaaaaa tgggcgaaac
     2821 gaatgcctag ttcgctgctg gctatcattt tggcaacagc agttatgcta ttgttccatt
     2881 taccagtagc aactgtgggt aagattccac aaacattgat cagtagtaat cgtttgaata
     2941 tgggagattt tagcttttct gccttgcaac aagtggcagt tccagctatt agcattgctt
     3001 tattaggaat gattgaaagc ttgctatgtg gtgcttcggc tggccgaatg gcaaataagc
     3061 ccttagatag caatcaagaa ttaatggcgc aaggaattgg aaatctgttg cttcctttct
     3121 ttggcgggat tcctgctact gcggccatcg cccgtacaag cgtggccatc aaatctggtg
     3181 cacaaactag attagctggt atgatacatg cagttgtctt actgctctcc atgcttgttt
     3241 ttgcgccagt gatgtcacag attccaatgc cagcacttgc aggtgttttg attgtgacgg
     3301 cttggcgtat gaatgaatgg gaaacgatca aggagctatt tactaataaa tattggtcag
     3361 cagttctatt gtttatcctt acgatgattt gtacagttat ctttgattta agtattgcta
     3421 tcgtgatcgg ggtgatcggc ggctgtgtat tttttgttgt caaaagcgct gctatcacga
     3481 tttcattaga aacgatcgat tggcaaagaa tggggctgcc agaaacggaa aagctgaata
     3541 actggacagt agtgtatatt agtggtccac tgttttttat gtcttcagaa aaactcaaaa
     3601 ataaattgga agaactaaat catcacgatg gtgtcatttt ctccatgcga ggcgtaccaa
     3661 gtattgattt gacggcacgt gcagtttttg atgaatttca agaaaaagca gagcaaagag
     3721 gacaaacgat tatctatact tccttgcagc cagaagtaga aaaacaactt tctcacttgt
     3781 gggaacatac attcacgcat caacatcaaa cagttgctca tgcgttgaca gctttacatg
     3841 cgaaatatac tgcaattagc cattaaaaca atgaaaaaga acagacaaga ctgtaaactg
     3901 aagattttat ggtcttgtct gttcttttta atattgctgt aaaagtgaag ctagtacttg
     3961 ccagtcttga tggataacag gtacaaggga gcgattgtag aaaatagctg ctggatgaaa
     4021 aagtggaaga attcgatagt ttttttctga ccattcgtag gcatcctttt gttctgttaa
     4081 ttgtaagaca ggccctgtat agagttgacc atgatttttg gtaaccgtat attctttgcc
     4141 aagtaaacgt tgaagaccaa tgtttccaag tgtcgcaatt aattttggct ggaccttcat
     4201 cagttcgtaa tccaatattg gtgcatgcgc aaatacttcg gaacgagtag gtgtccgatt
     4261 tggataaaca gtttctgact gttgtgtccg tttattgatc cgatgagtca cgcgatacgg
     4321 gcggctcctt acagcactgg tgatatacac ctcttctcgt gttaagccag ttgaagaaag
     4381 tgcctgcatc aattcttttc cagcttgacc gctaaaagga acatgcgtat caatctctgt
     4441 tttccctggg gcttctccca cgagcatcag tttaggatga agaggtcctt gcccagcgat
     4501 aaatccttct acaggacggc cgttcattct taaagaagct tcttgtatca gttcttttgg
     4561 gtattccatt tatgctcctc ctttttcttt tattataaga ataaaagaaa cacaatgcga
     4621 ctattttatt tggtagaact gtgaggatag aaaagcagaa gtattgcttg aatttttgtt
     4681 ttactttttg attttaaatt tagttgacta acggttgctg ttctggtaag ctagtactgt
     4741 tgtaattgtg gactccacag ctacaaccgc tcagaacaag taataagcac agcatatgct
     4801 gtcacttcgg atggcgagtc taagtatatc agaaggaggt gctttatagc atgtacgcaa
     4861 ttattaaaac tggtggtaaa caagtaaaag ttgaagtggg acaagcaatc tatgttgaaa
     4921 aacttgacgt tgaagcagga gaaaaagttg tttttgacga agttatctta gtaggcggag
     4981 aatctacaaa agtaggcgct ccaactgttg caggtgcaac tgttgaaggt actgttgaaa
     5041 aacacggcaa acaaaagaaa gtcgttactt tcaaatacaa acctaaaaaa cattcacacc
     5101 gtaaacaagg tcatcgtcaa ccatatacaa aagtggttat cgacgcgatc aacgcttaat
     5161 agcgtttgag aaagaaggcc tatatatgat caaaggaaca tttaagcgaa atgacgcagg
     5221 tcagatcgtt tcattcactt tgaccgggca tgctgatgct ggtccttatg gaagtgatat
     5281 cgtttgtgcg ggtgtttctg cgttagcgat cagcactgtc aacgggattg cctcattagc
     5341 cggttttgag ccgattgttg aaatgaatga agaagaaggc ggttatcttt ataccgaagt
     5401 cacttcagga atgacacaag aacaaaacaa tattgctgag attctattag aaaatctttt
     5461 attaggatta caatcgatag aagctgaaaa ctcagaatat atccaaatta aaacaattaa
     5521 tgataaatag gaggtgcaga ccatgttgtt atctatgaat ctacaattat ttgcccacaa
     5581 aaaaggtggc ggttctacat ctaacggccg tgactcagaa tcaaaacgtc taggtgctaa
     5641 acgtgctgat ggacaaacag ttacaggtgg atcaattctt taccgtcaac gcggtacaaa
     5701 aatctatcca ggtgtaaacg tgggtatcgg tggagacgac actttatttg caaaagttga
     5761 tggcgtagta cgtttcgaac gtaaaggccg taacaaaaaa caagtgtctg tttatccagt
     5821 agctcaagaa gcttaattga atcttatgat taaagaggtt gtgacaagac ttttgtcaca
     5881 gccttttttg tttataaaaa taaaagttgt agtggaggct acgaagttta ctttgtcttt
     5941 ttacacttaa tatttttttg ccaatttgga atcaggatgt tttgataact atcgttttca
     6001 attgatggaa gctatattgt ttgctatcag tgaaataatt atatggtaaa cagtgttctt
     6061 tttcttgttt ccattataaa aatcgtatcg ggatttttcg ttcttttaaa ttgaacattt
     6121 ctatctatcc gcgtgttatc aacaaaacaa aatcaggtaa atagttgacg aaacagaaat
     6181 aaatacatag tattatgttt tgaagcctaa gtacaatagt ctatagaaaa ggagcgatac
     6241 cattggcaaa acgcattgac cgaatttata tgtatgtcaa agaaaaaacc gctcatttaa
     6301 caaaggcaga atatgatcaa ggagtaacaa cacaggaaat tgctgaagtg ttagggattc
     6361 agcgaacaaa tagcagtaaa gatctgaatc agctagtgcg cgaaggaaaa ctgataaaga
     6421 cagacggacg accagtacgt tacatatatc aaacactcgt tacgcacgta aagccagtta
     6481 caaaacatgt agtcagttac aaagaaacgc cggtctctga agaaaaaaat acactccaaa
     6541 tagatactaa ggatatcttt gcaaaaatca ttggagctaa cggcagcatg aaaaattcag
     6601 tggaacaggc aaaagctgcg attttgtatc cgccaagagg attaaattgt ttgattacag
     6661 gacctaccgg atcaggaaag acttattttg cacacgcgat gtatcatttt gcaaaagcgc
     6721 atcatgtaat agatgaggaa acggaactga ttgttttcaa ttgtgctgat tatgccaata
     6781 accctgagct tttgatgagt catttatttg ggtacgcaaa aggtgctttt acaggcgcag
     6841 atgaagaaaa aatggggatt atcgatcaag cggatggcgg tatgctgttt ttggatgaga
     6901 ttcaccggtt gccgcctgaa ggtcaagaaa tgatctttta ctttatggat catggaactt
     6961 acagtcgtct tggggaaaca acgaaatccc atgaagcaaa tgtacggatc gtaggtgcga
     7021 ccacagaaga cccaggttct tctctattag aaacttttgt tcggcgtatc ccaatcaata
     7081 tcaaacttcc ggcttttgaa aaacgtcctg caaatgagaa agtcgattta gtaaaaatca
     7141 tgattgccca cgaagcaaac cgcacgcagc gaaagatttc tttgacagaa gatgtagcaa
     7201 aagcattgat cggcagtgtg acctatggaa atataggaca attgaagtca aatatccagt
     7261 tagtatgtgc acgaggattt ctaaatcata tgcaatcacc cgaaatctcg atcacaatcg
     7321 atgatttgac agaagggatt cgtagcggat tgattcaatt agcaagcaac cgcagtgcaa
     7381 tggcagagct ttctaaaata ttggagccaa agataactgt agcaccaaat gatacgatca
     7441 tgaaaatcca atcggactcc tatgagttac cttataatct atatgatatc atcggagata
     7501 aagcagcttt actgaagtcg gatggtttgg atcaagaagc aatcaatcat ttcatttcta
     7561 cagatatcaa catccatctg aagtctttct ataaagacca tggtttttct tttaatgcag
     7621 acagcaaatt agcagaattt gtagatccga aaatcataga agtgaccaat cagatttatg
     7681 taatggtcaa aagatcttta ccatatgagt ttcagcaaaa ctttatttat gctatgagct
     7741 tgcatatcag ttcttttttg aaaaaaatca acttaggaga agaacggcat acgaatgata
     7801 acatccgtga gatggctttt gattttccag atgaatatgc tgttgcgaaa gaagtgcgta
     7861 gttatatcga aacgtatttt caagtggaga ttccagaaag tgaagactat tacttgaccg
     7921 ttctactcgt ctcgttacgg gcaaatcaag caagcggcag aatcggtgta gtcgttgcag
     7981 ctcacggaaa cagcacagca agcagtatgg tccaagttgt ccaacagttg ttagatgcag
     8041 agaacgtacg agcagtagac atgcctttgg atatggatcc gaaaacagca ttggaccgaa
     8101 ttgaacgcaa tgtacaagaa gtagatgaag gaagcggggt catactctta gtagatatgg
     8161 ggtcccttgc gtcgtttaat tcgcaaattc aaagagacac tggaattcca gtacgtactg
     8221 ttgatatggt aacaacttct ctagttttgg aaactgtacg aaaggtctca gtattaggga
     8281 cagatctaga tatgttgcat gaatcattga aaaattttcg aggttacgca gaaatatctt
     8341 ctgtagaatc tgttcaggaa gcaaaaccta ttcataagaa aaaagctgtt ttggctatct
     8401 gcgcatctgg cgaaggtaca gcacaaagaa tcaaagaatt gatcgaacga gcggtaaaca
     8461 agcgtcaaga aacagaatta gaagttctag ctttgtcgat tgtggaagtg aaagaagaac
     8521 tgccagttat ccaaaaagaa tatcagatca tagctactac tgggattact gatttgaata
     8581 tccaagcccc atttattcct ttagaacgtt ttattgatca aaatatcgaa gtcattttgg
     8641 atcagttgct gatggaatca gaattggagg aaactgaatt catctcccta gatgaagagt
     8701 cagctaaaaa tacgtgtgtt gagtttatct ctgataactt catctttatt aatggtagta
     8761 agttaatcga tccaatgtgg caattttcta cacaaatctc tcagacgacg ggaatcggcg
     8821 atgaagaata tggattcaaa atcaacctag tgatgcacac agcaggaatg atcgaacgta
     8881 ttattcggaa tgaaccattg actgtggaag aaaacgaatt gacaaataca acgaatgatc
     8941 ctctttatag ccaattggca gcctcagttg ttctgttgga agatcagata aaagtaaaag
     9001 tgccgataga agaaatgtat tatctgctac gtctagtcca taatcagtta gataaaaaag
     9061 aatacactgt gccatgaata cacaaaaacg atacactttt atagtgtatc gtttttgtgt
     9121 attggttaag aatcctttaa attcaattag tgtatcgtaa aaaagttggc acgctttttg
     9181 cttatagtag tgtgtgctag gaggtggttg ttcacacaga aatgataggg gtttctgtga
     9241 agtagtgagg aagaaggagt tttgcagatg gatattcgat tggcacgtat agatgatcgt
     9301 ttaatccacg ggcaagtcgc aacagtttgg tcgaagcaaa caggtgttga acgtatcatt
     9361 gtgatcagcg atgtagtcgc tcaagataaa ttgagaaagt ttctactaaa agaagctgca
     9421 ccgccgggga tcacagcaaa tgtaataacc gtgcagaaaa tgattcaaat ctatcatagt
     9481 ggattattat cacgagcaaa agtgatgctt ttattcacca atccccaaga tgttgaagcg
     9541 gttgtccgct gcggtgtaca gttgaagtcg ttgaatatcg gcggtatgag ttttactgaa
     9601 ggcaaatcaa tgattaccaa ttttgtttca gtaaatcaac aagatattga ttcctttcgt
     9661 tacttgaaca gtcaagaagt ggagttagag attcgaaaag tacctgctga caggaaaatc
     9721 cttttgatgg aactactagc gaaaatcaaa aaggtgtagc aagaatggtt gttggaaaag
     9781 gtcaaataat tctgttgtaa tggttagcaa ttattaatat ttttgaattt gttacaacaa
     9841 aagtaaatag gaggtataaa tatggtaggg attatcctag caagtcatgg acaatttgct
     9901 gaaggaatct tacaatccgg ttcaatgatc tttggcgaac aagaaaacgt caaagccgtg
     9961 attttgaaac caagtgaagg tccagatgac ttgagagcca aattggaaga agcggttgct
    10021 tcttttgata atcaagacga agtgttattc ttagttgatt tatggggcgg aacaccgttc
    10081 aatcaatcaa acacattatt cgaagagcac aaagataaat gggcaatcgt cagcggtttg
    10141 aatttaccaa tgttgattga agcctacgca tcacgctttt caatggaatc tgctcatgaa
    10201 attgcagcac acatcattga aacagcaaaa gacggtgtaa aagtaaaacc ggaagaatta
    10261 gaaccggcag aagcaccgaa agcagcagtt gaagacgcac aacctaaagg agcacttcca
    10321 gaaggaactg tggtcggaga cggaaaaatc aaatatgtat tagcacgtgt tgactcacgt
    10381 ctgttacatg gacaagttgc gacagcttgg acaaaagcag tgcaaccaaa ccgtattatc
    10441 gttgtttcag atgcagtatc aaaggatgat ctgcgtaaga gactaatcga acaagctgct
    10501 cctccaggtg taaaagcaaa tgttattcct attagcaaaa tgatcgaagt agcaaaagac
    10561 cctcgttttg gaaataccaa agcattgctt ttatttgaaa atcctgaaga cgtattgaca
    10621 gctgttgaag gtggcgtaga catcaaagaa ttaaatgttg ggtcaatggc gcattcggtc
    10681 ggtaaagtcg ttgtaagtaa agtattgtct atgggacaag aagacgttga agcgtttgag
    10741 aagcttgaac aaaaaggtgt gaaattcgat gtacgtaaag tacctaatga ttcacgcgat
    10801 aacatggatg atattttgaa aaaagcaaaa gctgaattag caaaagcgta ataaataata
    10861 ttaaaattta taggaggctt atcatgtcta ttatttcaat aattttagtc gtattagttg
    10921 cctttctagc tggtatggaa ggtattcttg atgaatttca atttcatcaa ccactagtgg
    10981 catgtacatt gatcggttta gttaccggta acctcgaagc tggtatcgta cttggcggta
    11041 cgcttcagat gatcgcttta ggttgggcaa atatcggtgc cgctgtagca ccagatgctg
    11101 cattagcatc agtagcatca gcaattattt tagttttagg tggacaaggt gttaaaggtg
    11161 ttccttctgc gatcgcgatt gccgttcctt tggctgttgc agggttgttc ttaacaatga
    11221 tcgtacgtac aatcgctgtt ccaatcgttc atttgatgga tgcggctgct gaagaaggaa
    11281 acatccgtaa ggttgaaatg tggcacatca ttgctgtatg tttacaaggg gttcgtatcg
    11341 cggtcccagc agcagcgttg ctatttatcc cagcacaaac tgtgcaatca ttccttgaat
    11401 caatgccagc atggttaaca gatggtatgg ctatcggtgg gggaatggtc gtagctgtag
    11461 gttacgcttt ggtaatcaac atgatggcta caaaagaagt atggccattc ttcgttattg
    11521 gtttcgttgt agctgcaatt tctcaattaa cattgattgc tctaggtgct ttaggtgttg
    11581 cattagctct tatttacttg aacctttcta aaatgggtgg ttcatcaaat aatggaggcg
    11641 gaagcaactc tggtgaccct ttaggcgaca ttttgaatga ctattaatct tgaaggagga
    11701 aaataaaatg gcagaagaaa aaatcaaatt atcaaaaaaa gatcgtttag ctgttgcgtg
    11761 gcgctctacc ttcattcaag gttcatggaa ctacgaacgt atgcaaaacg gtggctgggc
    11821 attttctatg attccagcaa tcaaaaaatt atacaaaaca aaagaagaac gttctgcagc
    11881 attaaaacgt cacttggaat tctttaatac acacccatac attgcttctc caattttagg
    11941 ggttacattg gctctggaag aagaacgtgc gaatggcgcg cctgttgatg atgtagcgat
    12001 tcaaggggtt aaagtaggta tgatgggtcc tttagctggt gttggtgacc cagtgttctg
    12061 gtttacagta cgtccaatgc taggtgcact aggtgcttca ttagctatgg gaggaaatat
    12121 ccttggacca atcatcttct tcttaggttg gaacattatt cgttgggcat tcatgtggta
    12181 tacacaagaa tttggctaca aagctggttc taaaattact gatgaccttt ctggcggatt
    12241 gttacaagac gtaacaaaag gtgcttcaat tttagggatg ttcgtattag ctgcccttgt
    12301 acaaagatgg gtatcaatca aattcttacc agttgtatca actgtaaaat tagataaagg
    12361 tgcttacatc gaatgggata aattgccttc aggtggtgaa ggaatcaaga gtgcatttga
    12421 acaagttaat aatggcttag ccctttctcc tgaaaaggta acaacattac aagacaactt
    12481 agaccaattg attcctggtt tagctgcatt aggattaaca ttgttatgta tgtggttgct
    12541 taagaaaaag gtttctccaa tcgttatcat tcttggttta ttcgtagtcg gtgttgttgg
    12601 acacgtcatc ggacttttat aatccataaa aatatagctc gggcgttgtc ccgagctttt
    12661 tttatatagt atactgttca taaacaaata tctggaggga gaaaatggtt caatcaatca
    12721 atacaaaagt agacttggta attgatgcta cttcgtttac gggtctgaca gattacgggc
    12781 aaatcatgat aggtgataat ggttttgagt tttaccatgc gcgagataga cgcaaatata
    12841 tccaaattcc atgggaagaa gtggattatg tactagcatc agtcatgcta aagggaaaat
    12901 ggattcctcg atatgccatt caaacgaaaa aaaatggtac atacacattt tcttccaaag
    12961 aacctaaaaa agtgcttcgt gcgatcagag aatatgtaga tgaagataga atggtacaat
    13021 cattgagttt ctttgatgta gtgaaacgtg gtattcgtag tatgtttaaa cgcaagtaaa
    13081 aaataaagaa aacttctaag aggaggtttt ctttattttt ttactttgca gaagaaaata
    13141 tgaaatagca aaaccaatta tttgtcccct ttaaatccca tatgctaaaa ttgattagca
    13201 aagaaggagg ttatattatg agttggttat ggacattgat tgttggagcc gtgatcggag
    13261 caattgcagg tgcgatcacc agtaaaggaa attcgatggg ctgtgtaacg aatatcattg
    13321 ctggactagt cggttcaagt attggacaag cgattttcgg cgaatgggga ccaaaagctg
    13381 caggtatggc acttattcca tctattttag gcgcagtgat tttggtagca gttgtctcat
    13441 ttttctttgg aaaacgatca taagttttta caaaatagta aactaaaagg agttgcagcg
    13501 agactgtgac tcctttttgg ctttatttat ttaaaataaa aagaagtttt ccggaaattt
    13561 ttatttaact gtcgtgtacg aagaaaagtt tgctataatg gaaaaaagcg tgcaaagaaa
    13621 gagggagttt cattatgatg ctacgtgtag aaaaattgcg caaaaaaatg caagaagaaa
    13681 atttggattc atttttggtt accagtccgt acaatcttcg ttacttaacg aactttacag
    13741 gtacgacagg attagctgtt attactttgg aaaaagcatt ttttattact gattttcgtt
    13801 ataccgagca ggcggcagcg caggctcaag ggtttgaaat tattaaaaat gtcggaccta
    13861 tttttgatga ggtagcaaac ttagtacgca aagaagaact gcactcatta gcttttgagg
    13921 aaacaacagt ctcattttta gaatattcgg ttttagaaga aatcatcgat gcggaattag
    13981 tacctgtttc acagatgatc gaagaactca gagaagtgaa agatgaagaa gaaatcgcta
    14041 ttatcgaaaa agcatgtcat attgcggata tggcatatga tcacatcctg aaaatgatcc
    14101 aaccgggcat gactgagatt gaagtcgcaa atcaactaga cttttatatg cgttctcttg
    14161 gcgcttcagg tgtttctttt gatacgatcg ttgctagcgg attgcgttcg gctatgccgc
    14221 atggtgtagc aagcaaaaaa atcatcgaac aaggtgactt gatcacattg gactttggtt
    14281 gctattacga agggtacgtt tccgatatga cacgtacatt cgctattggt gatcctgggg
    14341 aaaaattaaa agaaatttat caaatagtct tagatgctca attagcagtt attgatgctg
    14401 caaaaccagg aatgactggt gtccaattag atgcagttgc tagagattat atttcaaaac
    14461 atggttacgg ggaagcattt ggacattcta caggacatgg gatcggttta gagatccacg
    14521 aaggaccaaa cgtttcttct cgtgcagaaa agcaatttgt accaggaaat gtgatcactg
    14581 atgaaccagg gatctactta ccaggaatcg gtggagtacg tattgaggat gacttgttga
    14641 tcacagaaga tggaaatcgt gtgttgactc gttcaccaaa agaattgatc atcttatagt
    14701 ttcatcaata gaagaagtaa gcgtaaaaat cagtgttcac agactttgtt atagaaaatc
    14761 actcgattta atgatacact atagaagaac ttttaagctg taagagataa taaagttggg
    14821 tgttttctcc aattcatagc agtttgcagc tgatcggcgg cagttttctt accacgtgat
    14881 cactatgccg catcatagag aattcggatg tatctcgtgc ttaggcaaag ggaggaataa
    14941 ataaatggca gatgaaaaaa atttgatttt aaacgcaaac caagatttag gagagatcgt
    15001 gatcgctcct gaagtcatcg aagtaattat cgggattgca gcttctaaag tagaaggcgt
    15061 atacggaatg cgcggaactt ttgccagcaa tgtcactgaa ttattaggac gtgcagctca
    15121 cggaaaaggt gtttacttag caaacgagga agatggcttg aaagtagatc tttactgtta
    15181 tctagaatat ggtgtttcag ttcctaaagt agcaatggaa atgcaggaac gggtcagcca
    15241 gcaagtcctt ttcatgacgg atatcgattt atctgaagtc aatatccatg tagtagctgt
    15301 tgttcctgaa aaattagaac aaccaacaat cgaagaactt tttgaagacg aagaggaaga
    15361 gaatgaataa agaattatca agacacgaga ttcgggaaat ggcattgcag gctttatttc
    15421 cattagactt caatgcagac ctaacaaaag aagatgcgat tttcaacgca atcgaattag
    15481 accaccgcga catgatcaac gaagatgaat cagagtttgt tcctgtttat cttgatacgc
    15541 tagttggcgg tgtctgtgca aaaaaagatg aattggataa agtgattgaa aaacatttaa
    15601 aaaacaattg gcacatcaat cggatctcaa aaatggattt ggtgatcttg cgtatcgcga
    15661 tttttgaaat gacttatgtg acagatgtac cagcggctgt tgctttaaat gaagcaatcg
    15721 agttggcaaa aacatttagt gatgaccgtt cacgtaaatt cgtcaacggt gttttatcca
    15781 atgtattgaa agaattagaa gctggggcgt aagtcctagc t
//
LOCUS       DAZUKC010000058        15801 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000058 DAZUKC010000000
VERSION     DAZUKC010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.059"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            29..781
                     /locus_tag="ACG80T_001861"
     CDS             29..781
                     /locus_tag="ACG80T_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS4841496.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            778..1692
                     /gene="pfkB"
                     /locus_tag="ACG80T_001862"
     CDS             778..1692
                     /gene="pfkB"
                     /locus_tag="ACG80T_001862"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFS4841497.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1710..3641
                     /locus_tag="ACG80T_001863"
     CDS             1710..3641
                     /locus_tag="ACG80T_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFS4841498.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(3752..5311)
                     /locus_tag="ACG80T_001864"
     CDS             complement(3752..5311)
                     /locus_tag="ACG80T_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFS4841499.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            5466..7700
                     /gene="pcrA"
                     /locus_tag="ACG80T_001865"
     CDS             5466..7700
                     /gene="pcrA"
                     /locus_tag="ACG80T_001865"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFS4841500.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            7717..9753
                     /gene="ligA"
                     /locus_tag="ACG80T_001866"
     CDS             7717..9753
                     /gene="ligA"
                     /locus_tag="ACG80T_001866"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFS4841501.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            9859..10164
                     /gene="gatC"
                     /locus_tag="ACG80T_001867"
     CDS             9859..10164
                     /gene="gatC"
                     /locus_tag="ACG80T_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFS4841502.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            10164..11636
                     /gene="gatA"
                     /locus_tag="ACG80T_001868"
     CDS             10164..11636
                     /gene="gatA"
                     /locus_tag="ACG80T_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFS4841503.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            11633..13063
                     /gene="gatB"
                     /locus_tag="ACG80T_001869"
     CDS             11633..13063
                     /gene="gatB"
                     /locus_tag="ACG80T_001869"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFS4841504.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            13073..14128
                     /locus_tag="ACG80T_001870"
     CDS             13073..14128
                     /locus_tag="ACG80T_001870"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFS4841505.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            14239..15612
                     /gene="rlmD"
                     /locus_tag="ACG80T_001871"
     CDS             14239..15612
                     /gene="rlmD"
                     /locus_tag="ACG80T_001871"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFS4841506.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            15615..15743
                     /locus_tag="ACG80T_001872"
     CDS             15615..15743
                     /locus_tag="ACG80T_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFS4841507.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
BASE COUNT         5373 a         2897 c         3469 g         4062 t
ORIGIN      
        1 tattatttgt attgaggtga tcgtgaaaat gcttacagag gaacgtagac aaaaaatcct
       61 agacttgctt gaacagcaga aaatcgtgaa atcacaagat cttgtaaacc tttttaatgc
      121 ttctgaatca acgataagac gtgatttaca agagctggag aatagtgggt ttctagaaag
      181 agttcatggc ggagctaaga aagagcaatt attgaatttt gagcagaata tgagtgaaaa
      241 atcactcaaa aacgttcacg aaaaacagca aatcgccaaa tcggcagctg aaaccattaa
      301 tgacgaagat gtcatttatt tagacgctgg ctctactacg ttagaaatga ttccttttct
      361 gcaaggaaaa cagatcacag tagtgacaaa ttccgttcaa catgcggcaa atttagttga
      421 tttggagctt gctaccatta ttttaggtgg aacgataaag ctggcaacta atgctgtttt
      481 aggatctaat gcgatagaac aactaaaaaa ttttcatttt aataaagcat ttatgggaat
      541 gaacggtgcc catcttgaac aaggattcac gactccggac ccagaagagg cagctgttaa
      601 acgtttagcc atgaaaaatt ccgaagagac atttgtattg attgatcata ccaaattcaa
      661 ccagttgtcc ttcacctctg ttgcaccttt ggaagcagcc acgatcataa ccgagcgttg
      721 tccattagaa ttcgttaatg aattccgtga aaagacaaca attaaggagg caatacaatg
      781 atttacacag taacacttaa tccatcaatt gattatatcg tacgcgtaga tgggttaaaa
      841 ctaggcgatt tgaaccgaat gaaggaagac ttcaagttac ctggaggaaa agggatcaat
      901 gtttcccgta ttttgaaacg gatcgattct caatctacag cactagggtt tttaggaggg
      961 ttcactggcg aatttatttc gaagtggctt gaaaaagaac aaatccagtc tttgttcaca
     1021 accgtaaaag aagatacaag aatcaatatc aaactaaaat cagatactga aactgaaatt
     1081 aacggtttgg gcccatctat ttcagatgaa gaaatcgatg agttgaagca agcattagga
     1141 acagtaggac aaggagatat cgtcgtattg tcaggaagcg cgccggctag cctgcgaaaa
     1201 ggattttatc aagaactgat tgatatcatt cgagaaaaag gtgcagattt cgtgattgat
     1261 acaacaggtg atgacctaaa aaatgcactt gttaagaaac cattgttgat caaaccaaac
     1321 aatcacgaat tagctgagct ttacaataca accttccaat ctgtagaaga aatcattccg
     1381 tatggtaaaa aattgctagc tgaaggggca cagaatgtgc tgatctcaat ggctggagac
     1441 ggtgctttgc tcttcacgga acaaggaact tataaaacga atgtattagt tcgcccattg
     1501 aaaaattcag taggtgcagg ggattcaatg attgccggct ttattggggc ttacagccgt
     1561 acgaaagatc caatcgaagc ctttaaatgg ggagttgctt gcggaagcgc aacagctttt
     1621 tctgatgact tggcagctcg cgaattgatt gatgagttgc tgccagaagt tgaaatcaca
     1681 aaaattgatt agtttcaagg aggataatca tgaatatcaa ggatttaatg gtcaaagaag
     1741 cgatgatcat ggatttgcaa gcgactgata aaaaaggcgc aatcgatgaa atggtccaaa
     1801 aaatgtttga tgccggccgg atttccgata tcgaaacata taaagaagga attctagccc
     1861 gcgaagcaca aacttctaca ggtcttggtg atggaatcgc catgcctcac gcgaaaaaca
     1921 gtgcagttaa agaagcaaca gttttattcg caaaaagcca aaaaggagta gattatgaag
     1981 ccttagacgg acagccaact tatctgtttt tcatgatcgc agctccagaa ggcgcaaacg
     2041 acacacattt gcaagcatta gcagcactat ctcgattgct gattgatgca gatttcgtag
     2101 gaaaattaaa agaagcaaat acaccagaag aagtccaaga cttgttccaa acagctgaag
     2161 agcaaaaaga agcagaagaa aaagcagaac aagaaacaca agctgcagaa gcttctgctc
     2221 atccagaaag aaaattcgtt gttgccgtta cagcttgtcc aacagggatc gctcacactt
     2281 acatggcaga agatgctctg aagaaaaaag cgaaagaaat gggtgtggag atcaaagtcg
     2341 aaacgaacgg ttcagaagga atcaaaaacc gattgactgc tgaagacatc gcccgtgcag
     2401 aaggtgtaat cgttgcagca gacaaaaaag tagagatgaa tcgctttgac ggaaaagaac
     2461 tgatcaatcg tcctgtcagt gatggtatcc gtaaaccaga agagctgatc aatctagccc
     2521 ttagtggtac tgcaccagta ttccatggtg atggaaaaga aagcagttca gaagaaggaa
     2581 atgcggatgg tactatcgga caaagaattt acaaagattt gatgaacggc gtatcacaca
     2641 tgttaccttt cgttattggt ggaggaatcg ctattgcgct ttctttcatg atcgaccaat
     2701 ttattggcgt gccacatgat caattatcaa atctagggaa ttacaatcaa gctgctagct
     2761 ggttcaacca aatcggacaa gctgcatttg gtttcatgct gccggtatta gcaggattta
     2821 ttgcttcaag tatcggtgac cgccctggtt tgatcgttgg atttgctgct ggtgcattag
     2881 ctaatacagg cggtgcagga ttcttaggtg cactgattgg tggattctta gctggttatg
     2941 tcatcgtatt tttacgtaaa ttattcaaga acttaccaaa atcattggat ggaattaaaa
     3001 cgatcctctt ttatccagtg tttggcttac tgattactgg tttcttgatg ttgctggtca
     3061 atgtccctat gaaagcaatc aatgatgcat tgaacggttt cttgacaagt atgagtggtt
     3121 cgaatgctgc tttacttggt gcattattag ctggaatgat ggcggcagat ttaggcggtc
     3181 caatcaacaa agccgcttat gtgtttggta cagcaacttt agctacgact gttgcaactg
     3241 gcggaagtgt cgtgatggct tctgttatgg ctggaggaat ggtacctcca ttggcaattt
     3301 tcgttgctac acgtttgttt aagaataaat tcagcaaaac agatcaagat gctggtttga
     3361 cgaacatcgt gatgggacta tcgtttgtca cggaaggatc gattccattt gcagcagctg
     3421 acccgattcg tgccattcca agttttatta ttggttctgc tttaactggc ggattagtcg
     3481 gagctttcgg gatcaaatta ctggctcctc atggcgggat tttcgttgta ttcttactga
     3541 gtcacccggt catgtatttg gtgtttattg ctatcggagc agtcgtatct ggtgtgattt
     3601 atggtttatt gagaaaagct cctgcagcag cagaagcata gaaataaaaa agaagaggct
     3661 ggtttttccc agtctcttct ttttttatag aatcatatag aatcaagtag tgtagatgaa
     3721 tagttgttgt aaaatagttt ttccatgttc gttaaaatga ttgcttcaaa tttgtgatag
     3781 aagatttagc ctcgatttcc tgcttgattc ttggcaagtt tgcttcatca gtcaagataa
     3841 ccaaaatatc tcctacattc atgatcgtat caccgtgagt caagatttcc tgatttcctc
     3901 tgcgtataga gattagcagc atatttttag gccaattgaa gtctcgtacc attaatccgt
     3961 ctaaagaact ctctgccgtt actggaaaat caaaagttgt ccttgcacct tttagtgagg
     4021 cggtctgagt atttccaagt agacgttcca ataatgattc gtaaattgga ttaccgccga
     4081 gaaggtcatt gacgatataa gcaacaagag cagccaatcc taaaggcatc agatgattaa
     4141 gagagccaac catttctgtc actaaaataa ttgctgtcag cggtgctttt ccaatcgctg
     4201 tgaaataacc agccatggca ataacgacaa aatccttgat gtagattgga tccatcccga
     4261 aatcaaccat aatgtttcca taaaagctac cgattaaagc ccccaatgtc aatatcggaa
     4321 ggaaaatccc ccctggaaga ccaccaccgt aagaaatcat ggaaaaaacg aatcgaagaa
     4381 caaacaaacc gataatcata atgattggca aggaagcatg tccaaaagct aaaacgattt
     4441 gattgcctcc gcctagagta ctaggccata aataaccaat tggcaaaatc agcaagaaag
     4501 gaacgaaccc gtgaaaataa gctggtaaag gcaatcggtt gtaaaatttt ggcaaagcca
     4561 gcaagacctt ctgataaata aaacctaaga tgcctagaag tactcctaag atcactaagg
     4621 aaccataata tttcaacggc aaagaaggaa ggtcacctag atataaaaca ggtctcaagc
     4681 cgaaaaagta aagagagacg aagttagcag tgattgctga agcaaatgat gtcagccaaa
     4741 caagtggaga aaaggagtga tggatttctt ctaaaacaaa gagtaatcct gcgataggag
     4801 cattgaatgc tgctgcaagt ccgctacttg caccgctaga aattaaaatc ttttcttctg
     4861 aagatggact ccgaaacaat tgtccagctc cttgtccaat ggatgcgcca agctgtatag
     4921 aaggtccttc tctacctaga aaaaggcccg caccaactga aagtacaccg ccaatgaact
     4981 ttttccataa aacagagaac cagttcattg ataattcccc gcgaagctgt ccctctactt
     5041 gtggaatccc gcttcccttg atatccggtt catttttgac cagataaccc acgaaaaatg
     5101 ctgcaaggat gcagcaaata gctaggccaa ttaagtaaat cggctgttcc cgactcattt
     5161 catagatact tcgaactaaa tcgcttagct tttcgataga taatcgaaac aggctaacga
     5221 cgattcctgt cagcagtcca acaaatattc cttttagaat aaaatatatc tgagtcttat
     5281 ctaatttctt gatttttcct tttttttcca ttcaaacgcc tcgctttctt ttaatttagc
     5341 gcgaaatcaa cgaattgaca aggaaaaatc cataaaaaga aacaaatgat tttgaactag
     5401 ctagtttaat gaaagccaag aaaatgctat aattaacaag attgataaaa gaaaggatgt
     5461 tgttaatgtc atccaaagct gaattattaa acgggatgaa cccccgtcag aaagaagctg
     5521 ttcttcacac tgacggacca ttactgctaa tggcaggtgc aggaagtgga aagacgagag
     5581 tgctgacaca ccgcattgcc tatttgattg aggaaaaaga agtcaatcct tggaacatcc
     5641 tagcgattac ttttaccaat aaggctgcaa aagaaatgaa agaacgtgtc aatgcgattc
     5701 tcgcatctgg aggcgaagat gtctgggtat cgacttttca ctcgatgtgt gtgcgtattt
     5761 taagaagaga tgttgatttt atcgggtata accggaactt tacaatcatc gattcttctg
     5821 aacagctgac cttgatgaaa cggatactaa aagaactgaa cattgatccg aaaaaatatg
     5881 atcctcgaag tatattagga acaatctctc aagcaaaaaa cagcctgcag acccctcagg
     5941 attttgcaaa aatgcaaggc agttattacg aagagattgc ggcaaaatgt tatgctgctt
     6001 accaaaaaga gcttcaatat aatcaatgta tggactttga cgacctgatc atgaatacga
     6061 ttcgattatt tgaagaacat cctgattcac tcacatatta tcaaaacaaa ttccattata
     6121 ttcatgtaga tgagtaccaa gatacaaatc acgcacagta tacgctagtc aatttattgg
     6181 caggtagatt taggaacctg tgcgtcgtgg gagatgcaga tcaaagtatt tacggctggc
     6241 gcggtgcaga tatgcagaac atcttggatt ttgaaaaaga ttatccagat gctgcagtaa
     6301 ttttgctaga acaaaactat cgctccacga aaaacatcct tagcgcagca aaccaagtca
     6361 ttgaaaacaa tagcaatcga aaaccaaaaa acttgtggac agaaaacaaa gaaggaaaca
     6421 agatcactta ctaccgtgca gataatgaac gagacgaaac ccgttttatc gtagatcgga
     6481 tgcaagaaga gattcgaagc aatcatcgga attacggcga ctttgccatc ttgtaccgga
     6541 cgaacgcaca atctcgtgta atggaagaaa ctctgttgaa agcaaatatt ccttataaaa
     6601 tggttggagg gcacaagttc tacgatcgta aagaaatcaa agatatttta gcgtatttga
     6661 atgtgctggc aaatcctcaa gattcaatca gttttgagcg tatcgttaat tcgcccaaac
     6721 gaggcatcgg accaggatct atcgaaaaac tacgcagttt tgcttctctt catgaatggc
     6781 cactgcttga agcagcgcaa aatgtggatc tggcaaatat cggtggaaaa gcaggacagc
     6841 aattaggtgc atttggagag atgatccaag aagtcactca gatgatccca tacttgacag
     6901 tgacagaatt gacgaaagag gttttggatc gtagcggata tctagaagac ttaaaaatcc
     6961 aaaatacatt agaagcacaa gcacgcatcg aaaacttgga ggaattttta acggtcacac
     7021 aagaattcga caaacaattc gaacagcaaa atgaagaaga cgctgatgca ccagaagaaa
     7081 aactaacggt cttcttaaat gatctggcac ttgtttccga tattgacaat ttagaagaag
     7141 atgcttccca agtgacattg atgactttgc atgcagccaa aggattagaa ttcccagttg
     7201 tattcctgat tggtctagaa gagggggttt ttcctttatc tcgagcattg atggaagaaa
     7261 gcgaactgga agaagaacgc cgtctcgcat atgtggggat cacgcgagca gaagaagcgt
     7321 tgtatttaac aaatgctttc tccagaacat tatatggacg gacgcaatat aaccgacctt
     7381 ctcgttttgt agaagaaatc gatcaggagt tactggaaat cgaaggcatg cgtcctacgc
     7441 ctaaaaaaac acctgtattc gctaaaaaaa cagcatatag ctataaacag ccagagacag
     7501 cggttgttcc ctctaaaagt gctactggag gggaaaataa cagctggaaa cctggagata
     7561 aagtcaaaca caaaaaatgg ggtctaggaa cagtcgtccg agtaagcggg acgtcgaagg
     7621 atctagaact agatgtggct ttcccaagtc aaggagtaaa acgtcttttg gctgcctttg
     7681 cgccaattga aaaagcttag tagtggaggg taaatgatgg aacatgaaat gacgattgac
     7741 gaagctgcta aacgagcaga agaacttcgt acaagactga accaatggtc tcgcgaatac
     7801 tacgtagaag ataaaccaac agtggaagac tatgtttatg ataaagaata cgcagaatta
     7861 gtagcaattg aggaacaata tcctgatttg atcacgtctg attctcccac tcaacgagta
     7921 ggcggaaaag tattagaagg ttttgaaaaa gtcactcatg atattccgct ttacagtttg
     7981 aacgacgttt tcagcaaaga agagctgata gcttttgatc aacgtgtaca aaaagcagtg
     8041 ggacgtgtag ttgactattg ctgtgaacta aaaatagatg gactttccgt ttctttgcgt
     8101 tatgaagatg gaaatttcgt gagaggtgcg actcgcgggg atggaactgt aggagaaaac
     8161 atcactgaaa acctaaaaac tgtccggtcg gtcccaatca aattaaaaga accaatgaac
     8221 atcgaagtaa gaggcgaatg tttcatgcca aaacggtcat ttgtccaatt gaaccaagac
     8281 agagaagcag agggaaaaga tatctttgcg aatcctcgga acgccgcagc tggttctttg
     8341 cgtcagttag attctaaaat cacagcgaaa agaaatctgg acactttttt atacacagta
     8401 gctgatttcg gtcctatgca agcaaaaacc cagtatgatg cgctagaaga gctagaaaaa
     8461 attggattcc acacgaatcg ggaaaaacgt ctatgtcatt cgattgatga agtgtggtca
     8521 tacattgaag aatatcatga caaacgtgtg gatttgccct atgaaatcga tggaatcgtc
     8581 atcaaagtga atgaattctc gctacaagat caacttggtt ttactgtcaa agcaccgaga
     8641 tgggcagcag cttacaagtt tccgccagaa gaagttgaaa cgctaatcga aaacatcgaa
     8701 tggacagtcg gtcgaacagg ggtagtaacg ccaacagcta tcatgacacc agtccgagta
     8761 gcaggaacga cagtaagccg tgccagcctg cataacggtg attatattaa gttgaaggat
     8821 atccgcttga aagatacagt tctgatttat aaagcgggag acatcatccc agaagtttcg
     8881 caagtcgtgt tagataaacg acctaaagac agcgaagaat accaattgcc tacacattgt
     8941 ccagtttgtg gaagtgaact tgtccatttg gatgaagaag tagccttacg gtgcatcaat
     9001 cctaaatgcc ctgctcaaat gaaagaaggt ttgaaccact ttgtttcaag aaatgcgatg
     9061 aatatcgatg ggttggggcc gcgcgtacta gaacagatgt atgataaaaa actagtagca
     9121 gatgttgctg atctttacaa attgacagaa gaagagttat tgacgttgga caaaataaaa
     9181 gaaaaatcag caaataatat tttgacagca atcgacaaca gcaaagataa ttcggtcgag
     9241 cggttgattt tcgggttagg aatccgacat gtcggagcga aagcagcaaa aattttagca
     9301 gaacatttcg gtgatctaga aacattgagt aaaagtgact atgagtcgat tattgcgctt
     9361 gatacgatcg gtgatatcat tgccgacagt gtagtcactt atttcagtaa cgaagaagtc
     9421 catgaattga tgaatgaatt aaaacaagca ggcgtcaatt tcgaatacaa agggcttcga
     9481 aatgcccaat tgcaagaagt cgagtctcct tttaaagaaa aaacagtagt ccttactggt
     9541 aaactaacac gatttactcg tgaagaagca aaagagacga tcgaaaatct tggcgggaaa
     9601 gtgactggta gcgtatcgaa aaaaacggat atcgtggtag caggagaaga tgctggcagc
     9661 aagctgacca aagcacaaga attaggaatc gaagtttgga cagaagatca aatggcagat
     9721 gcattggcta aaagtcgctc agtagaagaa tgaaaaatga gttttttcag acaaatttca
     9781 ataaacaatt tcagacacgg cactcttgtg ttaaaataga gagcaaagtt tttaactcgt
     9841 ttagaaagaa ggggtagtat ggcgattagt gaagaacaag tgaaacacgt tgccaaatta
     9901 gcaaaacttg cgttcgcaga tgaagaactt gcaagtttta ccgaccaatt aggtaaaatc
     9961 atcgatatgg ttgaaatgct ggaagaagta gatacagaag gtgttccatt tacgtcgaat
    10021 gtggttgaaa cagtaaacgt gatgcgacaa gatcgggcgc aagctggatg gaaccgcgat
    10081 gaattattga aaaatgtgcc ggaaacagaa gacggcttta tcaaagtacc cgcaatcatc
    10141 gataatgggg aggctggcgc ataatgacag aattacacaa tcgttctcta gaagaactgc
    10201 atcatatgct tgtatcgaaa gaaatcactg tacaagattt gacaaaagca acatttgaac
    10261 gaatcaaaga aacagagcct gaggtcgaag cattcattac attgaacgaa gaaaaagcat
    10321 tggaacaggc aaaagcttta gatgaaaaag ggattgatga aagcaacgtc cttgctggta
    10381 ttccaattgg tatcaaggat aatatcgtaa caaaagatat cttgacaacc gctgcatcta
    10441 agatccttta caattttgaa ccaatttatg atgcaacggt catggataaa atctatcaag
    10501 cagatatgat ccctgttggt aagctaaaca tggatgaatt tgctatgggt ggaagtacag
    10561 aaacttctta tttcaagaaa acaaaaaatg cttgggaccc aacaaaagta cctggtggct
    10621 cttctggtgg ttctgcagca gcagttgcag ctggacaaat tccagtttct ttaggtagtg
    10681 atactggggg aagtatccgc caaccagcag cttttaacgg catcgtaggg atgaaaccta
    10741 cttatggccg tgtctcacgt tttggtttga ttgcctttgc ttcatctctt gaccaaatcg
    10801 gaccaatgac ccgtacggtg aaagataatg cattagcatt gacagctatc agcggttatg
    10861 acgaaaaaga cggtacttct tcaggcgttt cagttcctga ttttgcagag ggactgacag
    10921 gcgacatcaa aggaatgaag attgctttac ctaaagaata cttaggcgaa ggtgtggccc
    10981 ctggcgtaaa agaagctgta ttgaaagcag cagaaacatt ccgttcatta ggtgctgttg
    11041 tggaagaagt cagcttgcct cattcaaaat acggtgtggc tgtttattat atcattgcct
    11101 catcagaagc aagttccaac ttgcaacgat tcgatggtat ccgctacggt tatcgctcag
    11161 aaaatgtgaa atcattagat gatgtatatg tcaactcacg ttcagaagga ttcggtgaag
    11221 aagtaaaacg ccgtatcatg ttaggtacat tctccttaag tgctggttat tatgacgctt
    11281 acttcaaaaa agcgggacaa gtccgtacat tgatcaaacg agattttgaa aaagtctttg
    11341 cagattatga cttgatcatc gggccatctt caccaacagt tgcattcgga atcggtgaga
    11401 acattaacga tccaatcaca atgtacatga gtgacatctt aacgatccct gtcaacttgg
    11461 ctggacttcc aggtatgtct gtaccggcag gtttatcaga aggattacca gttggattgc
    11521 agatcatcgg taattatttt gatgaaaaaa cgatgtatca agcggcatat gcgtttgaac
    11581 aagcaacaga attccataaa caacaaccag ctattttagg agggaaagac gaatgaattt
    11641 tgaaacagtc attggactag aagtccacgt cgagttaaaa accaactcaa aaattttctc
    11701 tccagcgcca gctcattttg gtgcagaacc aaacagcaat acaaacgtga tcgactgggg
    11761 ttatccaggc gtgctgccag taatgaataa agctgcgctg gaattcggga tgaaagcagc
    11821 tttggcatta aactgtgaaa tctctaaaga tactcatttt gaccggaaaa attactttta
    11881 cccagataat ccaaaagcat atcaaatttc ccaatttgat caaccaatcg gtcatgacgg
    11941 atggatcgaa atcgaagtag aaggtaaaaa gaaaaaaatc cggatcgaac gtgtccattt
    12001 agaagaagat gctggaaaaa acatgcatgg tattggcggt tattcttacg ttgatttgaa
    12061 ccgacaaggc acgcctttga tcgaaatcgt ttctgaagct gatatgcgtt caccagaaga
    12121 agcatacgct tatctagaag cattgcgatc aattatccaa tttacagaag tcagcgatgt
    12181 gaaaatggaa gaaggctcta tgcgctgtga tgccaatatt tctcttcgcc cttatggaca
    12241 agaagagttc ggtacgaaag cagaattgaa aaacttgaac tctttatctt tcgtgaaaaa
    12301 aggtttagct tttgaagaaa aacgtcaagc aaaagttctt ctttcaggag gagaaatcca
    12361 acaagaaaca cgccgttttg acgaaacaac gaataaaaca gttttgatgc gtgtcaaaga
    12421 agggtctagt gattatcgtt acttcccaga gcctgatatc ccgaaattcg tgatcgatga
    12481 cgaatggatt caaaaagtaa aagctagttt gcctgagatg ccagcttctc gtcgcgaacg
    12541 ctatatccgt gagtttggac ttccagagta tgacgcaatg gtattgacac ttacaaaaga
    12601 aatgtctgat ttttttgaag ctgttttgga aaatggtgca gatgccaagc aagcatctaa
    12661 ctggttgatg ggtgaagttt ctgcttactt gaacagtgag aaactggaat tagctgagac
    12721 aaaattgaca cctgagaact tagcaggaat gattcgtttg atcgccgatg gaacgatcag
    12781 ctcaaaaatc gccaaaaaag tcttccgtga attgatcaca aatggcggag aagcccaaga
    12841 agtagttgaa aaaaatggtt gggtgcaatt atctgatccg aacaaactac tgccaatcat
    12901 taatgaaatt ttggataaca accaacaatc tgtcgatgat ttcaaaaatg gaaaagatcg
    12961 tgcggttggc ttcctagtcg gtcaaatcat gaaagcaact aaaggacaag caaatcctgg
    13021 tgtcgtgaat aagctactta aagatgaatt agcgaaacga tagaggctga aaatgaaaaa
    13081 agcacgcgta atttataacc ccacctcggg aaaagaatta ttgaaaaaaa atttggcgga
    13141 tatcctacag gcattagaag aagcaggatt tgaagccagc gcctatgcaa ctacaccaga
    13201 accagattca gccaagaatg aagcatgtag agtcgcagaa ctaggttttg atctagtagt
    13261 ggcagctggt ggcgatggca caatcaacga agtggttaat ggaatcgctc cgcttgaaca
    13321 ccgtcctaaa atggccatca tcccagctgg tacaacgaac gactatgctc gagcattaaa
    13381 aattcctaga gataatatta aagcagcagc agaagtgata aaaaaacacc aaaccgtaaa
    13441 aatggacatc ggcctttctg ataacagtta cttcatcaat atcgctgcag gtggctattt
    13501 gacagaattg acttatgaag ttccatcaga actaaagagt atttttggtt atcttgctta
    13561 tcttgcgaaa ggagcagaga tgctccctcg agtaaagcca atcaaaatgc gcttgaagta
    13621 tgatgaaggg gaatatatcg gaaatgcttc gatgtttttc ctagggctga cgaactctgt
    13681 tggaggattc gaacagatcg cgccagatgc caaattggat gatgggaagt tttctttaat
    13741 cattgtgaag acagcaaata tctttgagat cttgcatatt gcagcactca tgctgaatgg
    13801 cggtcgacat gtcgatgatc caagaataat ctataccaaa acaagcagtt tgttcgttga
    13861 aacagaagat gatatcaatc gaccagtaat gatcaactta gatggtgaat acggcgggga
    13921 agcaccgatg catttccaaa atcttcatca acacattgag ttctttgcca ataccgatca
    13981 gattcctgat gaagcaatca caggtacaga tgaagaagaa ttagaggttg tcagcaaaga
    14041 attcgtaaaa gaagtcgaac gtttaacgga tgaagatatc gacggtgacg gaaaaatagc
    14101 agacgaaaca cctaatcaaa agaattaatc aaaagccggt gctgaacgta gtaaggtcag
    14161 tgccggtttt agttgtgtaa ttagctgatt tatagtagag ttcagtagga ttgaaaaata
    14221 ggcttaaagg agcaaaatat gacagaacag caaatagtaa agaaaaacga acgatataca
    14281 gtcgaaatcg tcgacttgag ttatgaagga atgggggtag caaaaatcga tggttatcca
    14341 ttatttatcg aaaatgcact ccctggagaa gaagtagaaa tactagtagt aaaagcaggc
    14401 aataagtttg gatatggaaa agtcgaaaca tttattacac aatcacctga tcgtcagcct
    14461 gtagaaaaca atgcattact tcgcataggt atcgcacctt tagcccattt gaagtatgaa
    14521 caacagcttg tcttcaaaca aaaacaagta gagcatgtaa tggcgaagat tgctaagatg
    14581 ccagaagttc ctgtacttcc aacggtcgga atggcagaac cattcggcta ccgtaataaa
    14641 gcacagatcc ctgttcgccg tatcgatgga cagttagcca ccggttttta caaaaagaat
    14701 agccatgatc tagtccctat cgaagatttt tatatccaag atcctgtcat cgatcaagca
    14761 atcaaagtag tcagagatat cttgcaacgg ttccaagtac gaggatataa tgaagaaaaa
    14821 aatgaaggac agcttcgtca catcattatc cgtcgtggtc attattccca tgaaatgatg
    14881 gtcgttttag ttactcgtaa agaaaaattc tttaaatcca aggaaattgc tgaagctatt
    14941 catgaagaat tgccagaagt ggtttcggtc atccaaaata tcaatgaaga aaaaacaaat
    15001 gtgattttag gagacaaaga aaaagtttta tatggcagag actatatcga agatcagcta
    15061 ttaggaaaaa cctatcgaat ctcagcaaag tcattttatc aagtaaatac tcaacaagca
    15121 gagtatctgt accaaacagc catcgagttt gccgagttag ataaaaaaga cattgtgatc
    15181 gacgcttatt caggaatagg tacgattggc ttgtctttag cagacagagt ggataaggtg
    15241 tatgggatgg aagttattcc agaagcaatt gaagatgcac agttcaatgc tttaaataat
    15301 aaaatcgaaa atgttcatta tgaagtcgga aaagcagaaa aagttatgaa aaaatggcaa
    15361 gaaaaaggga tcaaaccatc tgtgatcgta gttgaccctc cacgaaaagg attagatcat
    15421 cattttattg aatcagcaat cgaaatgtca ccagaacgaa tcgtctatat ctcatgtaat
    15481 ccggcaactt ttgcaagaga tgctaagtta tttgcagaag caggttatgc cgtgaagaaa
    15541 gttcagcctg tagatctttt cccgcaaacc catcatatcg aattagtcgc ttcattcgtt
    15601 aaaggagaat aaaaatggac caaaaagaaa aaatgaaaga attgatcaaa aagaaacaag
    15661 gtggaggccg atccaaacaa atggaaacac ctaagaatga tcgtaaaaac atgagaaaag
    15721 gccctaaaat ctttaataaa tagacgtgct aaagaaagac taagaagtgt taacttttgt
    15781 tattgtttct tagtcttttt a
//
LOCUS       DAZUKC010000059        15416 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000059 DAZUKC010000000
VERSION     DAZUKC010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.060"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            160..2220
                     /locus_tag="ACG80T_001873"
     CDS             160..2220
                     /locus_tag="ACG80T_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFS4841508.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2303..2872
                     /locus_tag="ACG80T_001874"
     CDS             2303..2872
                     /locus_tag="ACG80T_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4841509.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3047..3853)
                     /locus_tag="ACG80T_001875"
     CDS             complement(3047..3853)
                     /locus_tag="ACG80T_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841510.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3991..5505
                     /locus_tag="ACG80T_001876"
     CDS             3991..5505
                     /locus_tag="ACG80T_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFS4841511.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5908..6891)
                     /locus_tag="ACG80T_001877"
     CDS             complement(5908..6891)
                     /locus_tag="ACG80T_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFS4841512.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6896..7543)
                     /locus_tag="ACG80T_001878"
     CDS             complement(6896..7543)
                     /locus_tag="ACG80T_001878"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFS4841513.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7628..8260)
                     /locus_tag="ACG80T_001879"
     CDS             complement(7628..8260)
                     /locus_tag="ACG80T_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS4841514.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8276..8827)
                     /gene="coaC"
                     /locus_tag="ACG80T_001880"
     CDS             complement(8276..8827)
                     /gene="coaC"
                     /locus_tag="ACG80T_001880"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFS4841515.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8824..9561)
                     /locus_tag="ACG80T_001881"
     CDS             complement(8824..9561)
                     /locus_tag="ACG80T_001881"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFS4841516.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9558..10547)
                     /locus_tag="ACG80T_001882"
     CDS             complement(9558..10547)
                     /locus_tag="ACG80T_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFS4841517.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10805..11347
                     /locus_tag="ACG80T_001883"
     CDS             10805..11347
                     /locus_tag="ACG80T_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFS4841518.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11364..12449
                     /locus_tag="ACG80T_001884"
     CDS             11364..12449
                     /locus_tag="ACG80T_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4841519.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12461..13267
                     /locus_tag="ACG80T_001885"
     CDS             12461..13267
                     /locus_tag="ACG80T_001885"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4841520.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13268..14095
                     /locus_tag="ACG80T_001886"
     CDS             13268..14095
                     /locus_tag="ACG80T_001886"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4841521.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14092..15165
                     /locus_tag="ACG80T_001887"
     CDS             14092..15165
                     /locus_tag="ACG80T_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4841522.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
BASE COUNT         4956 a         2783 c         3140 g         4537 t
ORIGIN      
        1 agtacataag ttattattcc gattttcttg aaacaataaa aaatcaaaaa aaatactctc
       61 ctaatagtcc atcttctttt gattttattt atataatgaa atcatcttat gaaaattact
      121 acttcatgtt tagacaagag tagggaggac taagaataaa tgtgttcaaa aagacgttca
      181 aaacaactaa ataaacacgc ttgtcgttta gataaggaac ggtcgtgttc tgaaaaacac
      241 tacgttaagt ttatgatgcg cacgatgctg atcagtttat ctgtaggtgt ctatatgcat
      301 gtacccgttc aggcaacgac acaaaccatc aaggtcgtac gtacctctgt ttctgagacc
      361 actgattccc ctatcactat tactcatact ttcactgatg caaatggaaa acagctactt
      421 cctgcattga cttcctataa taaagtcagt tatcctccaa cgataaacca ctactggtta
      481 attcctaaca aaacattatg gacatcggaa accggaagca gtgtgccgct ttctatcttt
      541 tccgatatga ctgatcctac aaaaggtgat cagttaaaac agatcactga tattttgaat
      601 catgctttga cagatataaa ggacggtgga agcgcacaag tttcttatgt atatgaaaaa
      661 gattattcac agtttgaaag aaagaaaatc gatgttctgg cagggacaaa agtttcgata
      721 tctgatctgg tagaaaggct gaagaacgca gatggaacac aaggagatat gtccaaagta
      781 atttcaaaca atgggaatat cgtggatacg actaatacag gaacatctac tatttccttg
      841 tcctattttg atccaattgc tttaaaaaca atgactgcat ctgctgtgat ccatgttcgt
      901 gctgatcaaa cagctatcaa aggaaaagat cttcacttaa gaacaggaga gagttacgcc
      961 cctaaagatc ttgttgaata tgtaacaaaa agcgatggaa cttcaggaaa taaagaagaa
     1021 atcaaaatga acgggacagt agatactaca actccgggag aatatcccgt tgtattaact
     1081 tatatagact cgaaaacatc aaatgtggca gaaacaacag ctgtagttta tgtagaagca
     1141 tctgctgctt catcgactgt catcactcat tttatagata gtcaaacaaa taaagaactt
     1201 caagcagaaa cacatatgag tagtgtaaat cgtattcctg atttgcgcct acctgctgaa
     1261 ttttctgatc gagtgccaaa tatagattta tcgacttatg aacaaaatgg gaaagtcact
     1321 tctatttctg attggatgca agcaaacgga ttagacagaa aaagtcactg gtcggagatc
     1381 cttgaagtac ttcactcaga actgatgaat aataggaagc aatcaggagg ggaaattacc
     1441 tttacttatg tttatatacc agacaatcgt tcattagaag gtcatgatct tattgttcat
     1501 ccaaacgaaa aaataaacaa agaagagttg atccaatcag caacaaattc cttaggtaaa
     1561 cccgtaccgt taaaggatgt atcggttaca atcaatgaag taccattgga ggatggttat
     1621 ttgaccaaac aaacaggtga ttttacgatt atgtatagtt atcatgaccc gtattcactc
     1681 caagaactcc atgctcagtc actattacat gttttaccat taaacaaaca gatagatcaa
     1741 aaagaacaaa aaagacaaga agaaagcttg tcagaaaaaa aacaacaaat ggctcatgca
     1801 gtttctccaa cagggaaagt aaaacaaaca agaagcctag agttagcaag aatatctaga
     1861 aaaatctcag aatctaataa aacgataagg caagaagctc atttggcccc acaagtttct
     1921 ggattggaaa atgaaatttt agagcctaaa ataaaacaac aatcagaaaa agaatttgtt
     1981 gctaaacagt cggaagatca gaaggaatca gatgaccaga aggaaacgaa gaaaaaacgc
     2041 ttgtcaacta ttgattccac cgatgagcat ctgcagccgc aagtaccact tcctagtccg
     2101 ggaacacccg cacctggtgg aggtattcct tcttctgctt taggagattt catgcgaggt
     2161 atggcaggaa catgggtata cgctaatcga aatgaatttg acgcagatga ctggttctga
     2221 tttatatctc tatctgttat agtcaaataa gtagctgagt catatgttat ttggcataat
     2281 ggagacgagg agggatagaa aaatgttaga taaaacgtta ccttacgcag aaatctggat
     2341 gacaagacca cttcaacaaa atgttccaat cacgccttta gcggaaggtt ttgaatttcg
     2401 attttatcaa aaagaagatg aatctgcttg ggcggagatt gagacttccg ttggggaatt
     2461 tcaaacgaca gatgaagcac ttgagtattt tcaacaaaac tttgctcctt atcctgaaga
     2521 acttgaaaga aggatgtttt ttgtagagac aaaaaaagga gaaaaagtag caacctgtac
     2581 cgcttggcga aaaaaatcaa gagagcaagt gtatccggtt tttcattggt tagcagtgaa
     2641 accagattac caaggaaaag ggctcgcaaa agcattgact gccagagtct tgcaagaatt
     2701 tcctaccctc catacggacg gaccaatcta tcttcacaca cagacatgga gccatcaggc
     2761 gattgctctt tataaaaaaa tgggttttac ttttattgct gaaaatctag acggcacacc
     2821 taatccagat tatgaaaaag ttatgcaggt tttaagtaaa ttagataaat aacgaaatgg
     2881 tgaaataagt cctcagtatt gggataaaaa acgaaatagt cattcctcat atcttacttt
     2941 tttgagttaa tactatcgga taactaatgg catgccatac attcaaaaaa gaaggggcag
     3001 tgacaaaagt attgtcactg ccccttctct tgttttttag catgttttat cgtttgattt
     3061 ttttaggaat aaaattcttg atgaacgtaa atttttggta atcgtttcga ctatagaggt
     3121 aaccaaatac actgaaaaca actgctccaa taaaattaac aaataaatcc ttcatagtat
     3181 ccatgattcc cacgtctaag taaccatttt ggatgattgt tttatctgtt ttaccatctg
     3241 cattttgact ttcaatgatt gtccgagtga tatctggaac atgatagaca gtgtttccgt
     3301 ctttacttaa ctccacacta ttgatttgct ggatgatcgt atctttttgc atatcgaatt
     3361 gcagtaattt atctgctgaa aattccacaa actcccacat aacaccaact gtcatcgaaa
     3421 aacagaagga aacaactgct agtaataaag gagtcaagtt catggttttt gtatttttat
     3481 tgagcaaata aacaagggaa aagccgacac ccccagctaa aaatccgctg aatgcatgta
     3541 gaaaagtatc ccacattgaa aaatagccat aaaaatcact taactctcct aaaactgttg
     3601 aactaaaaat aaaagataac aaaatgattt ctagcatatt tggaatctgt atctttaata
     3661 tcttttccag aaaataaggg atttcaaata aaattaatgt cagtaataaa agcattgtat
     3721 ttccccagtg ttggttccag acctgatcca ctagcataat aacgacgatg atacgtaata
     3781 aaatatagat tccgtttgtg attctacctg cagtttttgt actgttcgta aaaaaggttt
     3841 tcaaatgatt cataaaataa ctccttcatg tatatcctta attatactaa gaagcgtgtg
     3901 tttaccaaag ctatatgact taaagaaaaa cagagttgat attgtgaaaa aaatcactta
     3961 atatagtata aggagaaaag ggggatacaa atgaaaaaat tagttgctgg tttattaaca
     4021 gccgttgcac ttcttgctat gagcgcatgc ggcgcagatc agaaaaatac agcgcagaca
     4081 agttctagta cgaaggaatc tgccgaagta tcatcaggcg cttctgaaca agtatataca
     4141 gatccaaaag aaatgaaaga atcttatgat gtcgtgatta ttggagctgg tggtgcaggg
     4201 atggcagcag ctatcgaagc aaaagatgct gggttgaatc cagtaatttt ggaaaagatg
     4261 cctgttgccg gaggaaacac atctaaatca tcaagcggta tgaatgcttc agaaacaaaa
     4321 tttcaaaaag aagaaggaat caaagactcg aatgatgcct tctatgaaga aacgctaaaa
     4381 ggtggtggag gaacgaatga taaagaaatg cttcgatact ttgtcgatca ctctgctgat
     4441 gcggtcgatt ggctagatca aaacggtatc aaactggaca acttaacgat cactggaggg
     4501 atgagtgaaa agaggacaca tcgtccgtct gatggctcgg ctattggtgg atacttagtg
     4561 gaggggttat tgaataacgt ccatgagcgg gaaatcccta tttttgtcaa cgcagatgtg
     4621 acagacatta agaaaaatgg ggaaacggtc aatgaagtga ctgtacatgt tcaaggagaa
     4681 gaaccaaaag aggtcaccgg aaaagcaatc gtagttacca caggcggttt tggtgccagc
     4741 aaagaattta ttgaaaaata tcgtcccgat ttagctgatt atattacgac aaatcaagaa
     4801 ggatcaactg gagatggtat caccatgatt gaaaaattag gtggacaaac cgttgatatg
     4861 gataaaatac aaattcatcc aactgtgcag caggatgagg gggtcttgat tggtgaggtt
     4921 gttcgcggag aaggagcaat cttggtcgat caagagggga aaagatttgt caatgaaatg
     4981 gatacaaggg ataaagtatc tgcagcaatt acagcattgc ctgaaaaatc agcttatttg
     5041 atttttgacc aaggcgtgcg tgatcgtgcc acagctattg aattttatga ccaaaaagga
     5101 tatgtcacaa ctggaaagac aatggaagaa ttagcagaaa aaatcgattt accaaaagaa
     5161 acactagcaa aaacagttga tggatggaat acggatgttg cggagaaaaa agatgctcaa
     5221 ttcaaccgtg ccacagccat ggaacatgac ttatcgtctc cgaattatta tgcgatcaaa
     5281 atcgcaccag gaatccatta cacgatgggc ggtgtgaaaa tcaatacgaa cactgaagta
     5341 ttagataaag aaaaccagcc aattaggggg ttatatgctg ctggtgaagt cacaggtggc
     5401 ttacatggaa acaaccggat cggtggaaat tcagttgcgg agatcattat ttttgggcga
     5461 caagcgggca tacaagcagc tcgatttgct tcggaaaatg aatgaaatag aacgtaaata
     5521 aaaagaggtt gtgaaatcag cgttttgagc taataccgag ggtcagctga ttgaacactg
     5581 tttagtgaag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca gccaagtaaa
     5641 aacagcttct tatgttttta acttggttgg acttattacc gcagatgcaa gcttttgaac
     5701 accgtttatt cggaaaaaga gcgtgggaca aaagtaaaga acacttttgt ctcacgctct
     5761 aaacacgtat aaacggcgga agcagaagaa attccttcgg aaataagctg aaattcacaa
     5821 aaatttgaaa agcaattttc gtgaatttct tcttatttct tggagttaaa cgcttctgtc
     5881 ccagcctctt ttttgatcag catgatttta ttcagttatc ttcaaaatga tttttccgat
     5941 gtttttgttt gcttccatat attcatgcgc ttgtttgact gcgctgaatt ggaatgtacg
     6001 gtcaatgatt ggccgaatct tgccattttc tagaaaaggc ccagcttttt gcataaaagt
     6061 ttgtgcgagc tctgctttat actgatcact tctaggtgtc aataatgtac caagcagttg
     6121 gacacgtttc ccaatcaata caccaaggtc tatctcaggc acgatggtcc cacctagcat
     6181 accaatcaag acccaacgtc catcaacggc tatagaagct aaattctttt cccagtaaga
     6241 agcgccgatg aaatccaaaa taacatcgac tccccgacct tcagtgattt ttcgtacctc
     6301 ttcttgaaag tcttgttttt tatagttgat caattcatct gcaccaagtt ctttacaaaa
     6361 ggcgagtttt tcttctgatc ctgcagtaac gatgattttc gcgtgagaaa gctgtctagc
     6421 taattggatc gctgctgttc ccacaccact tgctcctgca tggatcaaga cagtttcgcc
     6481 ttcttgtaat tttccaagcc agtaaagtgt ttgataagca gtcaaaaaaa cttctgagat
     6541 tccagcagca gaaacatagt ctagcgtatc agggagaagg attgcacgat cggcaggcat
     6601 cacagcatat tcggcataac cacctagatt gactaatccg tatacttttg tcccaggagt
     6661 aaaagtggag atatcacttt tattctcaac tacctctcca gctacttcga ctcccaaaat
     6721 agggtaggga ggtttcaaag agactgattc tcttttcata atatctgttc gattgacagc
     6781 tgctgtatgg actttgatca gtaattcgcc ttgttttggt ttaggaacag gcacttcaat
     6841 ttgttctaat acatctacag aaccaggatg cttgattgaa atggcacgca tggatctatt
     6901 cctcctttgc ccattcttgg aatgctgtat tcaactcttt gctaccttca gaaagtacta
     6961 gagtcgtacg agaaatatcc gtttcttcca ctagagcggc aagtacagca gcaactgtag
     7021 agcgagtgat cgtttttgat gccttctcat taggcttagt taattgaatg gaagtaactt
     7081 cttcatcgtt agtcaatgtg actggttgaa gaatgacaaa atctaagtta gttcgtttca
     7141 gccattcatc tgcataatgt ttagtaatat aataatctgt catggactca ggccattttg
     7201 ctggttcatc tgcataaaca gcactgacca tcagatagcg agagacatgc gcattttcag
     7261 ctgcgatcac tgttttgatt gcgccatcca agtctacttg cagaagattc tttccttgag
     7321 agccggcagc aaaaatcaat acatcgatgc ctttgaatgc ttcggtcatt ttttccacgg
     7381 accaagttaa atcaaaagag acatacgaaa cattctcttt ttttactaat gtttgggaag
     7441 caacatctct cacccctgca aaaatttcat gggaacttgg agctaaatct tttatcaggt
     7501 gtcggccaat ttgtccgttt gcacctacaa caaaaatttt catttggatc atcctttcga
     7561 cgtcaaatct ttccattcaa tagtaacacc aaaagagaaa aaaatcagag atcggctacg
     7621 atgactatca gttagtaaga gaagggtgct tgaagtatct taacaagaca ttgcatactg
     7681 ctgcagtcgc aagacctgcg acgattcctt caggtatacc acttgtaaaa ataaccgcta
     7741 gaatagctga ataaacagca tttaaatttg ttccgatgac tttggcataa tcttgctgga
     7801 ataaaaagta gatcatattc atcactaaaa tcgtattggt caatgaaccg gaaagtccag
     7861 ctaagaataa agaaacggaa tctgcttttt gtttcattag tttgcgcaga cctttataaa
     7921 cgaaataagg aaccacccca atcaagatgc gaggaataaa cgcgatcagc aaggctttcc
     7981 agcttccatg ttctgttccg atgactggga taaacggaga aaagacaaaa gacaaaggtg
     8041 tttggataac tgtgctacgg atcatgctga tcagtccaaa tgtaccgcca agaaaagcac
     8101 ctagtcttgg tcctaaaacg atcgaagcaa tgattacagg aatatgcatg gttgttgcat
     8161 tgattggacc gatagggata aaacctaaag gcgtaacagc taataaaatc aaaatagcta
     8221 aaaacatcgc agttaatgta aagttttttg tatttttcat aagtactcct tttttctaag
     8281 catttctttc attaagagtc agcatgacct gttcgatgat cgtctgaata tcagctaagg
     8341 caccttttcc aaaatctcca caagctaaaa gagattctct tggttcaatt tcctgatagc
     8401 cgacagatct caatgtttcc agattacgct gagtgatcgg gttttgatac atattcgtgt
     8461 tcatggcagg ggcaatcaat tttggtgttc ccactggaag cgccattgcg actgttgaaa
     8521 gaagatcgtc tgcaatgccg tttgctagct tgccgataat gtttgctgta gcaggcgcga
     8581 tgagaaacaa gtcagcttgt tttgctagtt cgatatgatt gattacctgc ggatttgatt
     8641 cttgcatgac gtctgtatgg actgcgcgct tcgacagaga ttgcaaggtt aatggcgtga
     8701 taaaatgtgt gctgctttta gacataatca catctacctg ggcacctaat ttgacgaatt
     8761 gacttgtaag atcagcgctt ttgtaagcag aaatacttcc agtaacgcct agtaagatct
     8821 ttttcatttt gattcctcca gtctaatttt ctcagtaatc aatgcagcaa tctcagattt
     8881 actttgagct tcttccactg ttccatcttt agataagaga tagccgtggt gtgtctcgcc
     8941 atggatttcc gtcaaatcat ttgcgaaaat aaaatcagtt ttgttattta tcagactatt
     9001 ttttgcgact gccagaagag attcctttgg tacatctact aatagtttga aaccaatgat
     9061 ttttgctttt ggctgccatt cacgtagata agaaatgatt tttggtgttt tcttcaaaac
     9121 gagcacaaga tgctcggtgt tagaagagat ctttgaaaca gtggtatctg tctgttcatt
     9181 ttccgcaaac caattgtcca gttcttcttg cgttgaagaa gtgggtaggt tttttgccag
     9241 ctgttcttct gaaaaactaa aagcaggagt aaaatcactg actgccatac tgtggataat
     9301 cgcatcgtag tgttcttttt tcgttagttc ttctaattgt aaaaataagt ccagtgcaga
     9361 ttcgatcgta taaatcgtta tattggagtg tcttttcggt ttcagtgctt gtttcgtagt
     9421 cacgtaatcg attgtggtat ccggattggc agctaaataa tcagctaatg cttgccctaa
     9481 tcgtcctgtc gaatgatttg taatagcacg tacttgatcg attttttcag tcgttcctcc
     9541 agctgtgatt agaatcttca tttatggtca cctacattta ctagtgttct tccttggtgt
     9601 gttccagcaa gtagtttaga tgctgtttca ggcaattgat ccagagtaat ctcttggacg
     9661 accagttcat ctgcaatctg caaatccgcc atctgttgcc ataaagaaag acgttgatct
     9721 attggtacat tgacagaatc aattccaatc aactggatgt ttcgcagaat aaaaggcaag
     9781 acagtcgcgt ttagtttgat tcccccagca ttgccacata aagctactgc accgttataa
     9841 gagatcaatg gaagaagact gcttagctgc tcgccaccta ctgtgtcgat gacatagtcg
     9901 atttgctgtt ttcctaaagc ttttgttgtc tctggcagaa attcatcagg tcgtacgact
     9961 tgcaaagcac ctagactttt tagccatgtg acagactctt cttttctgga aaaggcaatg
    10021 attgactgga atcctagttt atgtaacagg gctatcgctg tactgcctac accgccagaa
    10081 gcaccagtaa cgacaacttt tttatcggag gtcatacctt gacggattaa agcgttcaca
    10141 cacaacatag cagtaaaccc tgctgtacca aggatcatcg cttgacgcag cgtcatattt
    10201 tttggtagag gaactaacca gtctcctggt actttttgat attggctata accgcctgga
    10261 tgggtgacgc caagcccaaa accagtagca agtacaagat cttcttttga aaaacgtgga
    10321 ttgttacttt ggacgacagt tccggataaa tcaatccctg gagtcatcgg atatgttcgt
    10381 atcacctgac cagactcgct cattgccaat gcatctttat aattgacatc agaataggca
    10441 atttttacca agacttcatt ttcagccaac tcagtaaatg gcagatcact gattgctgta
    10501 tggaaaacag gttcctttat tacctgaaaa gatcgaaaag cgttcattcg tgagacactc
    10561 cttttaaaat tttcttttat cataccataa agcctactct tttattcttg ttcttccgta
    10621 aatttagaca aaaattaggt gaaaaataaa gaatcaaaca actagaattt attccaaaag
    10681 ataaagaaat attcagcaaa aaaaatttta caaagggttg cctttttcaa taaatttcgt
    10741 atactaatac gagtggtagt aaacaggaag tttactatta gaaaacttcg attcggagga
    10801 aattatggaa attggtgaga agttaagaaa tttgcgcatc caaaagaatt tgacacaaga
    10861 agaactaggc gaacgcacag atttgtcaaa agggtatatt tcccagttgg aacgagattt
    10921 aagttcacca tcaatggaaa cattcttttc tattcttgaa gtcttaggtg tgactccaga
    10981 agaatttttt catcaagaag cagcgaatct gcaagtggtt tattcaaaag aagatcatac
    11041 ggtttatttc gatgaagaaa acgggtatga attggaatgg ctcgtggctg attcgaatga
    11101 aaaagaaatg gagcctgtct tgctgacttt tgaaggtgca ggagaatata agacgtttga
    11161 accttcatta tctgaaacgt ttatttttgt attagaaggg gaattagagt taagcttagg
    11221 agagacggtc tatgtggcaa aagcaggaca gtcgatctat tatcaagcga caaaacaaca
    11281 tcaattaagg aatcatctca aaaaacgaac aaaagtattg attgttgcaa cgcaatcata
    11341 tttgtaatat agggggtaaa aacgtgggga aaccaatcat ttcatttgat caagtagtga
    11401 aacgctatga tgatgaaaca gtattaaaaa aagtaagttt tgagatcgaa caaggaaaat
    11461 tttatacact gcttggtcct tccggctgtg gtaaaacaac gatcctacgt attatagctg
    11521 gattcaccga agctacagaa ggggacatct attttgaagg gaaacggttg aatgatttgc
    11581 cagctaataa gcgacaagta aatacagtct tccaagatta tgcacttttt cctcatatga
    11641 atgtttttga taacgttgct tttggattga agatcaagaa aatgtccaaa tcagatatcg
    11701 aaaagaaggt aaaagatgct ttgcgtatgg tccagcttcc tggatacgaa aaacgggaga
    11761 ttagtgaaat gtctggtggt caaagacagc gtgtagcgat tgcacgagca attgtaaatg
    11821 aaccgaaagt attgctatta gatgaaccgt tatcggcgct tgatttgaaa ctacgtacag
    11881 atatgcagta tgaactgcgg gatctgcaac aacgattagg aatcacgttt atttttgtta
    11941 ctcatgacca agaagaagcg ttggcaatga gtgatgagat ttttgtcatg aataaaggga
    12001 aaatcgtcca aagtggtaca cccgtagata tttatgatga gccaatcaat catttcgttg
    12061 ctgattttgt tggggagagc aatatcgtgg acggtgtaat gatcgaagat aatctagttg
    12121 agtttgttgg taagcaattt gaatgtgtcg atggcgggat gcgtctaaat gaaccagttg
    12181 aagttgtttt acgcccagag gatttgacga tcacgacacc agataaagga aaactagtcg
    12241 taacggtcga tactcagctg tttcgtggtg tccattatga aatcatctgt tatgatgaac
    12301 agcaaaatga atggatggtc cattcaacga aaaaagctaa agaaggaagc aaagtaggat
    12361 tggcctttga gccagaagat atccatgtca tgcgtttcaa cgagtctgaa gaagaattcg
    12421 atgcacgtct tgacagctac gaagaatagg ggggacaaga atgaaaacaa tgcgcaggat
    12481 ctattccgtc ccttatattt tgtggctcgg tttattcgtg atcgcgcctg tattaatgat
    12541 catttatcaa tctttttttg atatgaatgg gcaattcacg ctgaataatt atctatctta
    12601 ttttacgtcg gggacatcgc tttctatgac gatcaattct gtatggtatg catttttgat
    12661 cacgtttttc acgctgatca tcagttaccc gactgcttat tttttaacaa aactgaagca
    12721 caagcaatta tggctgatgc tgattatttt gcctacatgg gtgaatctgc tgcttaaagc
    12781 ttacgcattt attgggattt tcagtgtgca cggcagcgtg aatcatttct ttgattttat
    12841 cgggatcggg ccgaaacaga tattattcac agacttcagt tttctgttcg tggcaacgta
    12901 tatcgagatt ccatttatga ttatgccaat tttcaatgca ttggaagaga tgaatccttc
    12961 tttgatcagt gctagtagag atttaggcgc gaatagcttt gagaccttcc gacgagtgat
    13021 ttttccgtta tcattaaatg gtgtgaaaag tggtgtccaa gcagtattta ttccttctct
    13081 ttctttattc atgctgactc gtctgattgg cggaaatcgg gtgatcactt taggaacggc
    13141 tatcgaacag catttcttgg ttacacaaaa ctgggggatg ggttctacaa tcggtgtgat
    13201 tttaatcatt gctatgttca tcgtgatgct cctgacaggc gagaagaaaa aaggaggtcg
    13261 ctcataaatg aaaaaattta aatggtcgta tctttattta gggatcgtct ttttattctt
    13321 atatgcaccg atcttctatc tgatttttta ttccttcaat gaaggaggaa cgatgaacag
    13381 ctttacagga tttacttggg aaaattatga agctgttttt gaagatacac gtttgatcat
    13441 tatcgtattg aatacgttca tgctggcttt cttatctgca ttgatcgcca cgatcatcgg
    13501 gacatttggg gctatgggaa tctattacac gaaaaaacga cgtgcacgga cagcgttgtt
    13561 gagcatgaat aacatcttat tagtctctcc tgatgttatt atcggtgcta gtttcttgat
    13621 tttctttaca ctactaggat tcggattagg ttttcagagt gtattgcttt cccatattgc
    13681 tttcagcatt ccaatcgtcg tcttgatggt cttgccaaaa ttgcaggaaa tgaacgattc
    13741 aatggtcgat gcggctagag accttggtgc taataatttc caagtagtca aaaatatcat
    13801 tttgccttat ctttctccag gaatcatagc tggatatttt atggcattta cctattctct
    13861 tgatgatttt gcggttacat ttttcgtcac aggaaatggc tttacgacgc tctcagtaga
    13921 gatctattct cgtgcaagac aaggaatcag tttagaaatc aatgcgctaa gtgcgttagt
    13981 tttcttgttc tccatgatat tggtcattgg atactacttt attagtaaag aaaaccgtcc
    14041 aaaacggctg aagaaaaaca gaagagtacg cgagggggtg gcacggctgc gatgaaaaaa
    14101 ttacaatctc tgtttttagg tattctcatc attattgcgg tgctgctgct tggtgttcgt
    14161 caacttgaaa aaacaagtgg catgagtgga gcaaaggtac ttaccatcta taactgggga
    14221 gattatatcg atcctagttt gatcacgaaa tttgaaaaag aatatggata caaagtgaat
    14281 tatgaaacat ttgattctaa tgaagcgatg ttcacaaaaa tccagcaagg tgggacaaac
    14341 tacgacatcg ctataccaag tgaatatatg atccaaaaaa tgatcaaaga aaaactagta
    14401 cttccattag atcattcgaa gatcaaagga ttaaataata ttgatccacg atttttagac
    14461 ttggattttg atccagataa tcagtattcg attccatatt tttggggaac tttagggatc
    14521 gtatataacg ataaattctt tgatgcaaat caaattaagc attgggatga tttgtggaag
    14581 ccggaattaa aagacagttt gatgctgatt gacggtgcac gagaagttat ggggctttct
    14641 ttgaatagtt taggctattc tttgaatagt aagaatatgg cacagctgac agaagcagca
    14701 aataaattga atcatttaac accaaatgtc aaagcaatcg tggcagatga gatcaaaatg
    14761 tatatgatca atgaagaagc ctccgtggca gtgacatttt ctggcgaagc tgccgatatg
    14821 atgtgggaaa atgaacattt acattatgtg atcccaccag aaggatccaa tctttggttt
    14881 gataatatcg ttatgccaaa aaccgtgaaa aataaagaag gtgcctatga tttcatcaac
    14941 tttatgctag aaccggaaaa tgcggcacaa aatgcagagt atattggtta ttctacacca
    15001 aatgaagaag caaaagcgtt attgccaaaa gacatttcgt cggacgaaca attctatcca
    15061 tctgatgaca cgatctccca tcttgaagtc tacaaagatc taggatcgaa atacctaggc
    15121 atctataatg acctattctt agaattcaag atgtatagga gataatggca aaactcagta
    15181 agaatgggga cttatcaacg ttctaaagag ctaaaaatcg tcctttttct atctgagttt
    15241 tccaatcttc taccaaaagc ggatgaaacg ccgctttttt tgtgttattt tattttttct
    15301 accacgtttt tctaccagag aaaagtggac aaaaccattg taaaataggg cccgagacgg
    15361 gctagtaaat ctaagattga aaaagttaca gaaggataaa gtaattggaa actatg
//
LOCUS       DAZUKC010000060        14815 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000060 DAZUKC010000000
VERSION     DAZUKC010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.061"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(296..1024)
                     /locus_tag="ACG80T_001888"
     CDS             complement(296..1024)
                     /locus_tag="ACG80T_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4841523.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="ACG80T_001889"
     CDS             complement(1002..2576)
                     /locus_tag="ACG80T_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFS4841524.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="ACG80T_001890"
     CDS             2805..3743
                     /locus_tag="ACG80T_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4841525.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="ACG80T_001891"
     CDS             3755..4666
                     /locus_tag="ACG80T_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4841526.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="ACG80T_001892"
     CDS             4690..6162
                     /locus_tag="ACG80T_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS4841527.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="ACG80T_001893"
     CDS             6213..6824
                     /locus_tag="ACG80T_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS4841528.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="ACG80T_001894"
     CDS             6854..8767
                     /locus_tag="ACG80T_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HFS4841529.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="ACG80T_001895"
     CDS             8895..11876
                     /locus_tag="ACG80T_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFS4841530.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="ACG80T_001896"
     CDS             11873..13534
                     /locus_tag="ACG80T_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HFS4841531.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="ACG80T_001897"
     CDS             13574..14506
                     /locus_tag="ACG80T_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFS4841532.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4815 a         2333 c         2978 g         4689 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagt
//
LOCUS       DAZUKC010000061        14073 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000061 DAZUKC010000000
VERSION     DAZUKC010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.062"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            46..321
                     /locus_tag="ACG80T_001898"
     CDS             46..321
                     /locus_tag="ACG80T_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841533.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            complement(318..470)
                     /locus_tag="ACG80T_001899"
     CDS             complement(318..470)
                     /locus_tag="ACG80T_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841534.1"
                     /translation="MKLSVEGTPEEIQELLQAIGSSKEQKVSVSNSDIDGICNCLNRE
                     KISNPY"
     gene            complement(779..1192)
                     /locus_tag="ACG80T_001900"
     CDS             complement(779..1192)
                     /locus_tag="ACG80T_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFS4841535.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            complement(1269..1565)
                     /locus_tag="ACG80T_001901"
     CDS             complement(1269..1565)
                     /locus_tag="ACG80T_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841536.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            complement(1562..2119)
                     /locus_tag="ACG80T_001902"
     CDS             complement(1562..2119)
                     /locus_tag="ACG80T_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFS4841537.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            complement(2116..2535)
                     /locus_tag="ACG80T_001903"
     CDS             complement(2116..2535)
                     /locus_tag="ACG80T_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFS4841538.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            complement(2532..2777)
                     /locus_tag="ACG80T_001904"
     CDS             complement(2532..2777)
                     /locus_tag="ACG80T_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841539.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            complement(2737..3093)
                     /locus_tag="ACG80T_001905"
     CDS             complement(2737..3093)
                     /locus_tag="ACG80T_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFS4841540.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            complement(3093..3398)
                     /locus_tag="ACG80T_001906"
     CDS             complement(3093..3398)
                     /locus_tag="ACG80T_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841541.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            complement(3395..3556)
                     /locus_tag="ACG80T_001907"
     CDS             complement(3395..3556)
                     /locus_tag="ACG80T_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFS4841542.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            complement(3553..3855)
                     /locus_tag="ACG80T_001908"
     CDS             complement(3553..3855)
                     /locus_tag="ACG80T_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841543.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            complement(3848..4012)
                     /locus_tag="ACG80T_001909"
     CDS             complement(3848..4012)
                     /locus_tag="ACG80T_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841544.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            complement(4017..4286)
                     /locus_tag="ACG80T_001910"
     CDS             complement(4017..4286)
                     /locus_tag="ACG80T_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841545.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            complement(4298..5047)
                     /locus_tag="ACG80T_001911"
     CDS             complement(4298..5047)
                     /locus_tag="ACG80T_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4841546.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(5050..5736)
                     /locus_tag="ACG80T_001912"
     CDS             complement(5050..5736)
                     /locus_tag="ACG80T_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFS4841547.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            complement(5742..6413)
                     /locus_tag="ACG80T_001913"
     CDS             complement(5742..6413)
                     /locus_tag="ACG80T_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFS4841548.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(6406..6747)
                     /locus_tag="ACG80T_001914"
     CDS             complement(6406..6747)
                     /locus_tag="ACG80T_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841549.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(6925..7623)
                     /locus_tag="ACG80T_001915"
     CDS             complement(6925..7623)
                     /locus_tag="ACG80T_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFS4841550.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            7710..8180
                     /locus_tag="ACG80T_001916"
     CDS             7710..8180
                     /locus_tag="ACG80T_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841551.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(8223..8402)
                     /locus_tag="ACG80T_001917"
     CDS             complement(8223..8402)
                     /locus_tag="ACG80T_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841552.1"
                     /translation="MGKFNRALVFSAPLIIYALELWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(8389..8727)
                     /locus_tag="ACG80T_001918"
     CDS             complement(8389..8727)
                     /locus_tag="ACG80T_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFS4841553.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(8743..8946)
                     /locus_tag="ACG80T_001919"
     CDS             complement(8743..8946)
                     /locus_tag="ACG80T_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4841554.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(8961..9737)
                     /locus_tag="ACG80T_001920"
     CDS             complement(8961..9737)
                     /locus_tag="ACG80T_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFS4841555.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            10036..10356
                     /locus_tag="ACG80T_001921"
     CDS             10036..10356
                     /locus_tag="ACG80T_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4841556.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            10374..10805
                     /locus_tag="ACG80T_001922"
     CDS             10374..10805
                     /locus_tag="ACG80T_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFS4841557.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            10935..11396
                     /locus_tag="ACG80T_001923"
     CDS             10935..11396
                     /locus_tag="ACG80T_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFS4841558.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            11430..12065
                     /locus_tag="ACG80T_001924"
     CDS             11430..12065
                     /locus_tag="ACG80T_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFS4841559.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPSTEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            12178..12813
                     /locus_tag="ACG80T_001925"
     CDS             12178..12813
                     /locus_tag="ACG80T_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841560.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(12806..12940)
                     /locus_tag="ACG80T_001926"
     CDS             complement(12806..12940)
                     /locus_tag="ACG80T_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841561.1"
                     /translation="MIKVIFKRITELPEFRLFVDYWYVWFFIVALLIGLDVILERKLK
                     "
     gene            13112..>14073
                     /locus_tag="ACG80T_001927"
     CDS             13112..>14073
                     /locus_tag="ACG80T_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4841562.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHT"
BASE COUNT         4285 a         2682 c         2216 g         4890 t
ORIGIN      
        1 agcgagtggt ccgctgaaat ataaaacaag gatgtgcaag acaacatgta taaaccctac
       61 atgattagtt atgatcttaa taacccaggg caaaagtatg ataaagtatt tgaaataata
      121 aaagaatttg gggcatatat aaagctacaa aaatcttttt ggttagttaa aactaatttg
      181 aatccaaatc aaatgtgtga aaaattaaat acagtacttg ataataacga ttccttattc
      241 atctgtgaac tgcaaaaaaa ttatcaaggt agagctacag aggaaaattg gaaattcatt
      301 aacgaacata ttttctctta gtaaggatta gatatttttt ctctgtttaa acaattgcaa
      361 ataccgtcaa tatctgaatt agaaacactt accttttgct ccttgctact accaatagct
      421 tggagcaatt cttgtatttc ttctggagtg ccttctactg ataatttcat ttttcttccc
      481 tccaatacat aaattaatag acagcagcag ttgaaagacg acaagaacat gtttagtttt
      541 gtgagtgctg ctgtctaatc aaaataaaca gcaaccgatg aagaatttag gaggagttga
      601 attcacatcc ttttcttcat aattagttgc tgtctatcta agaagaagtt taaaacgatg
      661 agggagattg cctcccttcg tttattttgt cgatcctgtt tcctaatctt tcgacactac
      721 cataataaca ctggtaaatg tctaaaaacc gccatcattc cgccaaaaaa ccgccaaatt
      781 atttataagc aattattttt ccgtgtttat atgcttctgc aaactctatt agagcttccg
      841 acttcatccg ttgtatactt ctttctgaat aacccacttc acggctaatt ctgtagtttg
      901 agaagctatc tggcacacag aagctgtagt agagtatctg acgactaatc agactaagag
      961 ccatcaaagc cgctagaatc gcatctctct ccgtttctat atccatcatc tgaataatcg
     1021 cgtcttctgc cttattgcca tgcttcggtg ccttcggcat atccgttata atcggcgact
     1081 taatatctat caaagagcga cctgccatcc gctccaaacg ccgaaagttc ttcagcacat
     1141 ctctcgcatt acatcttgtc tgtttgaaat ctacctctcg taacaattgc atcaagtcaa
     1201 accgctcctt tatgtgatat aataaatgtg ttggatttat tgaatcagtc ggagcgatcc
     1261 ggcttttttt atttgctctt gattacttcc atgtcaacta atctcgctac agctaaataa
     1321 tgcttgtgtt ttgcagtcga acgatcacac ttcattgtat tttcaatacg aatgattgct
     1381 gagtttgaca gtatcttttt aacgtatcct ctgaacggat aaacgaactc ttctgcttca
     1441 cagcggacca tgtcaccgac tttgaatttt gatttcttgc gtgttttagg gttctttgtc
     1501 ggcatatcta gcattaaacc gccgataccg tgactgctag cgtaaaatcc gtcttttagt
     1561 ttcatctttc taccacctct tccactgttc cattcaccaa caatgctgca gcatccgctg
     1621 ttgctttatc agtgaaaagc aatcgatgag atggattgca ttcaaaacta taatttactc
     1681 catcttttcc ccataaagca catttagtaa gatagtttcc atcggcattc tttattgccc
     1741 ataacggctc tttctcaacc tcgtagccgt tgtataggct caatagtgtt tcatatgatt
     1801 gtcttgttgc ccaagcgagt aattcttctc cttgctcttg cgtcactatg ccatctcgta
     1861 caaaccaatc acagaaataa tatgaatcac catctttaga tttgattaaa tacgctattt
     1921 tttcagcttt tgtaaaaggg tccgtagatt tctcaagcca accggccacg agttgtggaa
     1981 taactggttt ctgcggttcg tctatttttt tcattaacgt aatacctgaa tttatactct
     2041 cgttatagca agcagctatt gcatcatttc ctatcgcttt aatactttct aactttttga
     2101 ttgcttcctg tttattcatc gctgttcctc caataactcg ctattctcgt atatatttcc
     2161 ggtgacttcg tacgtgtatt cttcaaacag ctctgtgtta aaaatttgat actctaagtc
     2221 ttcatcaaca gtggcacaaa ctaatccgga ataatctatt gaatttttga caatacagac
     2281 tttattatct aagtaatcga agccatttcg cacgctgact aatactatat ccccttcaaa
     2341 tatatccaca ccattcttat ctttcattcc tgtggattgc atgaggtcga tgtttcgcac
     2401 gtgatatgat ttcaattcgg taggatttat ccaatattca ataaagtttg ttttgccgtt
     2461 tttagtaaaa tgcaagacgg ctacatctct cattacgttc tttcgtttat cccacgctct
     2521 aaacttcggt atcattcgct gtcctcctcg tatttttcaa tcaattccat tacttttttc
     2581 actatttcaa actcaaccgc ttttgattct tcgaaatcat gaacaatttc tggaaacagt
     2641 acatcatcaa ctacccacaa aataatctgg cgctttccac caaaatttat gattagatgg
     2701 tccgattcca cagatagtgt tgctcctgat tcgatatcat ataaatccat gctgaattga
     2761 acgagttttt ttatcatttg ctgtcctcct caaatactct tctaatattt ctttatactt
     2821 ctctacaaat ttgaaacgat cttgatgaag tttcttgctc caatttgttt gccgatccag
     2881 ctcacgcatc tgatcgaacc ctttttgaat ttcgttgtaa taaaattcaa tgtttgctgc
     2941 tgctttccaa tgcctgctac ttcgcactcc tgctcctgtt tcagccattt ctaacttaac
     3001 taattcagct cgttcttttg attttttatc tttctgaatc ttcatcatga ttttcttgag
     3061 gatgatgtca ctgtattgtg taatgagatc cattatctct cctccacata cctaaactgt
     3121 cgtccttttg aatcaatcca taagctccta gctctatccc agataatgtt tttgcttaat
     3181 ccagtaattt cagataactg ttcagcagta cctgttacta aaattcggtc atcatgccag
     3241 attgcaattt ttctcggcgt tctccgtttg ggcttttcag tccacattga tttaccgagc
     3301 ttttggactt ctgcaactat ttctttgtct tcttgccaag attctgactt ggttaattca
     3361 gcaattcttt tcattgctgc tttcttatcc acgctcattc ctccaatcga tggatttccc
     3421 ttcttaaatt ctctatgtgc aaatcgattg cctttctcgc cgtttcattg accatcactg
     3481 cctttgttcg ttccagatcg tcaatctcac gctgaaggct tcgaatacgc atttgaatca
     3541 cttcttctgt tgtcatgatg attcctccac gtatctaaac gttctcttct tagcgtctgt
     3601 gtagccacac cttgctcttt tcctcacgat tttctcgtgc aagcctgtga gacttgctaa
     3661 ctgttcagct gtccctatca ctagaagttt gtcaccgtgc caaattgcga tttttcgtgg
     3721 tcttggcttg ttgctcttgt ctgcccacat cgctcttcca agcctcatca cttctgaagc
     3781 agcttctttg tcattttgcc aatcttctga ataagtcaat tcgataattc gctgcattgc
     3841 cgctttctta tccatcccga cattctcctt tcagtaattt gagtacttga tcaagtgcgc
     3901 tctcacgtcc gccatggaac gtgttgagcc acttgtcttc gtacgataca ctttgtctta
     3961 aagcttcttg atgcattagt tcaatctgtg ctgtaaatgt ctttaggtcc atctgattac
     4021 acctgctcaa gttcattaag atgtttttgc aagcctttaa cacaatcaac aaatagtaat
     4081 ttggtatatg ctaaatttct taattgtgtt gtatcgatat aaagtgcgaa atagtatctg
     4141 agcttactcc aactcgaacg atcattctta attccttcaa ttccagcttc ttcgagttga
     4201 tcatatacgt ctctcaggac gtctaattcc tcgccctttt tataattcgc tatttcatta
     4261 attagttcta gataatcgat cttcattttt ccacctctta gaatggtgct tttgattgtc
     4321 tattagctcg ttctagcgct tttttcttga gataggcttc ttggtcgatt gcccattcag
     4381 gaagcttctc tcgtcttcct gtgcgcttgt atccactgct tgcgttctta ggttcacttt
     4441 tttctttcct tgcccaactt cgaatagttg ccaaatagtt tttataagtc ttaccagatg
     4501 attcacaata ctctgacagt cgttcgattc gctcttggta atcattaggg aattctgttt
     4561 tgagtttctc catctgctca tctgacaaaa gaacattttt atactctccg tatttatgac
     4621 ggacgggctt agccttcgat tttttcgaag gcggtaactc tcttatatat tcttttgtat
     4681 tattaaatgt attattaata gatgtattat tatctttgac tttttcgtca ataggggtat
     4741 tgcgtttttc gtcaataggg tattgattaa ttcgtaggta cctattgatt atttgattgg
     4801 taccctcttt gtaaatgatt tcccgattca agtatccaaa cttaatcaaa tcacttaccc
     4861 atcgcgatat ggtctcttta ttcacaccat ataaatctgc aaagtactca ttgcctgccc
     4921 aacaaaagcc tctttcatta cacaaggccg ttatctctcc gtataacaac ttagtatttg
     4981 gtttaagtct tttgtcgtac cttacgttgg ctggtataat cgcataataa cttcgatgtt
     5041 ctgtcatttt taccctccaa tatttaactt tttgattgtt ttctggttta atttgatccc
     5101 tttgatttga tatttatttt tgaaattaat cacacctatt ttgtgcttct ctgtgtgatg
     5161 gattctgcag agtgctgcaa atgtgtactc tgcatgatca acttctttgc gctttcgtct
     5221 tcctagcgct ttgtcaaagt gatcgatgtc agctcctgtt ttgccacaga tgcagcagac
     5281 tctttttgtg atgcatttgt agaagtaata ttcttgattc gcaggtaaaa tctcatagcc
     5341 ttctttgaaa ggaatatgat gttcaaagat gaaatctaag atgatatttg ctaagacatt
     5401 ggcatcactc acagttgtat tcgattcgtc tttcaggctt attttgcgcc ctgtgacacc
     5461 ttcaaaacga aagtagaaga attccttcca gaagtccgtt ggcatgcctg tatcgataaa
     5521 aatatcgcct atcagcgcat agatgaagtt tcgttgctgt acggtgaaac gtctaggatc
     5581 aataaaacga atttcaatga ctcgatcacc atcgtagccg tcatacatcg tctttagtcg
     5641 atcaatgttc acttcctcat tgatggttgc gcttatgtct tttcctttga actttttcag
     5701 aaccgctgaa tatgaatcga ttaatggttt aaacactcat atcacttctt atctaattct
     5761 tttctcttag ctgctattgc tcgctccatc aaggcacatt gctcatagct taactgttca
     5821 atagtttcaa cgttatcagc taagagccct aatttatctg tctgctcatt aacatattcg
     5881 attaaggttt tggtcatatc tttacccatc tgctcattga aagcttctag aatcgtctct
     5941 agcatgttta atttctttgt atcgattcta ggtggtgttg gaatatcttc cccttgaaat
     6001 acatataatc ccagtccgtg tagagccaat gctttcacaa agcatcgctt caatgagtta
     6061 ttgatttgca ttgcatttgg tttaacaact ggttggtttc gataatctaa aacaggaaat
     6121 aattcggttt ccgtgtgtcc tttaaccgtt actgagacag atacataagt cccagtttca
     6181 tccataagaa aaggtttata ttcctcaaca agaaagtctt gatgagttcc agaaacaacc
     6241 ctgtagtgtt tgtactcatt aatagttacc gttgcctgtg gatcattctt tttcataatc
     6301 tcccacgcgt gagcccaaga taaataatca aaatttcctt ttttcttgag aattttattt
     6361 aacttacgac taaaaagttt ttcaaagttc gttgtccctt tgatttcact catcaaattc
     6421 tgcctccatt tcagcaatgt atttcttacc tggtccgtaa taagagatat caatcaagtt
     6481 atctctgtcg tactcttcta gcgcatcaat caagccatct tcgatgacgt aaatatattc
     6541 aggtttattc gaatgcttcg atagatggat aagataaaca tgatcccaaa tagttacatg
     6601 gttgcctaaa tcatcttgat cccaagctag ttcttcattc gtcaaaagat ttcgtctgat
     6661 ttttcgacca cttgtttcct caattttcgg cttgccccaa tcaggatcaa tcaaatattg
     6721 atctagagtg gaaagttctt tttccatatg ctaaaatctc cttatgatgt gttttctttg
     6781 tgactctttg cttgccggcg gagtcacttt tttatttgtt gccatgcttt ttgcttgtca
     6841 atatgttgtt gacttaggat gattggttta tagtatttcc accagcaatt agcaattgcc
     6901 gtccctattc ttagcgcttc agctctattc attgtcatca ccaaaaagtc tctgttgtct
     6961 gttcagttga tcaatttcca tacggatcgc agtttctggc aaccacattt caataaatga
     7021 aacagcatca tcgaatctct tacgaggtaa ctcgccatat cttgggattg aaaaggtacg
     7081 tttaaattca gaccaaaatt ttgagaatac ttttttgctg atttcttcat aagctcggct
     7141 ttcttttccc cctagaactc ccataacttt catatttcct ttttgcttaa tttcaaactc
     7201 ttgttgtccg ctaattcgca tagtatcttt aagcatggaa acatcttttt taacatcttt
     7261 catttcttct aattgataga tcatcatatc ttcaattgtt tgaggaacag tattcttgcg
     7321 aataacatct tccatttcgt tgaatgcttc aatgtatttt tgtttgaagt agatagcttt
     7381 ctttcctgta aaccccatag ccagcaagaa aaatccatct ctactaatga agaaaactcg
     7441 tcgatttctg ccatatgaat ctggttcatt accttctaca aacatctgtc caaaattgga
     7501 cacatctttt tttagtgcat caatatctct taaaacatgt tgatgttttt tctcgaagct
     7561 ttctgccact tgtaagctcg tagtcacagc ttctttattt ttcaaaatta ctaattcttg
     7621 cattatttct tctctccttt ttggtataat ttagttaaaa actggtggtg tttattttga
     7681 atttttttca cttaacaatt tttcaatgga tgtcaataat aagttttata ttgagtacgt
     7741 ttttaacagg gataaaaata aaaggaatgc gccctcaact agaagttgaa cttaatgctt
     7801 cttacttcgc tgcagatatg atttatacaa aagtaatcat ttctaactat tcaacagaac
     7861 cagctatgtt agttaattta gaactttcat ccgctaactt aaaccgcaga tggtcagcta
     7921 ctccatttaa gaaattaatt gctaaaggag gatcagtcga tgataataga atatactctg
     7981 aagctgtacc gctaaatatt cctcctaaaa gtgctatatc ttgttacctt gcatttgaag
     8041 tagggaaaat taattttaat aaactcctta atcaccaaac aaaaatgata tttactctaa
     8101 atagaaccca aatccacaaa attgttgata tcaaaaatac aaacttccca atagagaaat
     8161 tagtgaaaga attaaactga gtaatagtaa tatacattct agtttctctt ttctattcat
     8221 ttttagtcag ccccctcggt tggctttttc gctctgtact cagcttcagc ttcatcaagc
     8281 cccataaaaa tccaaaccat gtaaacaatc gttcctatca acgcttgcct gcttccccaa
     8341 agttctaaag cgtagatgat tagcggtgcg ctgaacacta gcgctctatt aaattttccc
     8401 atccgcttgc ctccttaaga tttccgaaaa atttgtttct aaaaattcaa gtgttttact
     8461 tcttaaaaat agatatgtgt ctcttccttc aactggataa tagacaaatc catttttgtt
     8521 tttctcgata tcgataatat ttctatatct tgggttcttt aaaactctag aagtaaacca
     8581 atcatatttt ctgttaatcc gttctagcac ttctggcaac gtcatccatc taccagtatc
     8641 atcagctttt tttaactcct cataatccac ttgggagata attacatagc cttctggaat
     8701 tgggattttt gcttctagat attgcattag ctgttcctcc ctttacaatt cgtacatagt
     8761 gataatcgaa tctataattc tgtttgcttc tgcagaagtc tttttaccgt ttaaaattaa
     8821 agataagtag cttttgctaa ttccaaatct ttcagcaagc atggtgtaag ttaagaactt
     8881 tgaactttcg acatattctt tgattttttc tctatctcgt tgagtgattt ctgcaatgtc
     8941 agtcatacta aaactccttt ctaaccaatt tcctctaaat ccatttgagg gtaatatcct
     9001 tcttttttta gtaattcgta gataaataga cgccctttct gtgtccattt ggtattcatt
     9061 acaattttag tgccaccatc ggctttcggg atctcagttg tatgagattt tgtgtatcct
     9121 tggttcatgt gttttttgca caataaccat tggttaccga cttttttctg aatacctagt
     9181 ttatgaagta atttattcat ctgttgtgga gacatcccat aatctgctgc aatctgacta
     9241 attgttactg aatctgtaga agataatatg ctatctaaat aggagatttt aggttcgtac
     9301 tctgcaatct tttgttctgc gattaatctt ccagttcttt cttcttttag ttgagttgct
     9361 aattgaatga ttgtatctgg attaagcaaa gcttcttcta ctttttctgg agttagataa
     9421 cctccatgtt ttctaattgc tggcaacact tcacttgtta cccaacgttt aaattttttg
     9481 gcagagggaa gttttgattt taagattaaa ctgtacaaac cagattcgtt gatgattgtc
     9541 atctctcttg attgacctga ggtcgtgatt cgcgacccca tcttatcttc caaatctaca
     9601 tgccgcgaca aagcatcttt agtgtttgaa taacccaaaa cactcgcaac gtctttgcct
     9661 acaaaatatg gttcatcatt tactaaaatt gtccgaactt cgttttgttc gaaattaaaa
     9721 atttgcggtg tgttcatatt tctcattcct ttcttttata ttcgtaaaca aatttaacaa
     9781 cttttttcta aattcaattg acagtatctg agtaatactc tatactaaga gcataattaa
     9841 ataagacata aaaacattga ttttaaagct ttcttggcgg tctgcattta catatcaata
     9901 gtgttttttg ttgtcttttt agttgttaaa tctgtttaca agatatagta tagctcatta
     9961 ctcagaatta gtcaaattat ttctgcgtat ttttctaaac tttttttgta aacatttaga
    10021 aaggttgttt tatcgatgaa tacctatgaa ataataaaag acttggcgaa gaggaaaaag
    10081 atatctatac ggcagttaga aattaatttt ggatattcta acggttatct agcaaagtgg
    10141 aaaaataata ctcctaattc agatgaatta agtcggattg ctgactattt cgatgtctct
    10201 gtagactacc ttctaggtag agaagaaaga gaaaccccta aacatgtgga tttatcagaa
    10261 gacgatactg ttttttcttt tgatggaaaa gaaatatcta aggagacaat gcgtaaagcg
    10321 attgcaattg ctaaagcttt agaggaaaat gaatagttgg agtgatggtt tgtatgtatt
    10381 tgaaattgaa agaaatgttg agcgaataca atttgaaagt agtttatatg gagatgaaag
    10441 agcctggttt ctactatcca aaacccagaa tcattttttt gaatgaaaat ctatacggtg
    10501 aaactgcaga agcctttcat ttatctcatg aacttgcaca tttcagcgcc tctcattttg
    10561 aattttcagt tttgtacgat acttctacaa cttttcactc aaaatttgaa acagaagcag
    10621 ataaaatagc tattctaatt ttattaaata tctatattga gaacgaactg actgatgaat
    10681 ctcagtttaa tctcgaaaaa tttatggagt attattctat tcagaataag ctcagataca
    10741 cttgttatgc tgtctgccaa tgctatttta agaaaaaata ttcttacgca aggcaatatg
    10801 tatgaataca tcaagaatta ttaaaaaaac cgatttcagc aaaacattaa aaaagcccgt
    10861 gctgcaacac gggctctttc ctcatatatg agcttctaca aaaaaaatca tatcacagaa
    10921 atgaggaaga aaaaatggaa aaagagattt taataaaatc tccaggaaaa accttgatta
    10981 aagtaactga tgattctatt tcaattatta gaaaaggatt tataaattta gttaatcaag
    11041 gtattaaagg agaaaaaact atccccttta aaaatatatc agccgtacaa ttaaaaaaac
    11101 caggtatgag taatggttat atacaattta ccttgctagg cggaaacgaa agtcgcggag
    11161 gtatattagc ggccacgaaa gatgaaaaca ccgttatgtt tacaaaaaaa tattggaatg
    11221 aaatggaaaa tttaaaaaaa tatatagaga agcaacaatc tatattagat aataattcca
    11281 aagaaacaca aatttcaagt gcagatgaaa ttaaaaagta taaagagttg ttagatgaag
    11341 ggattattaa tcaagaagaa ttcgatttta agaaaaaaga attgcttggt ttataatatt
    11401 caatatcaaa agagaaagag gatctattaa tggacggtat atttggtttt actggtattg
    11461 ttttatttat tataggttta atcatgttaa ttgtaagatt tatcaaaaaa acaaataaga
    11521 aaacacctat gattctcctt attatcggga ttatttttac taccgtaggt ttctcgcttt
    11581 cctcatcaaa cgaggcaaat gacaataaca aacaggaaag ctcatcatct gatactcaaa
    11641 ctactacatc taacagcagc aataaaagcc taactcaatt tgaagaattt ttagagacaa
    11701 ataatcatga ctggggtgct ttcttagata gttattattc tattactcca tctactgagc
    11761 aaaatacagc ttttactaaa tatattagtg gtaaaacata tacttttgaa ggaactgtta
    11821 tcgaatcaat gactactaga atagccatta tagcagacaa agagtacgat gataaaagtt
    11881 ggaatgatat ttctgcaaca cctaaagttt catatgtaat ttttgcgaag gacttaaaca
    11941 atgctgatac ctttacaaag ggcgataaag taacttttac aggagaaatt agttcaagag
    12001 gatcgaacat agaaaaatcc tatgctcaat gggatatgat taatagtaaa gtaagtaaaa
    12061 aataaatgaa ccacgcccca ctaagagtta gattgtgcac aataataaaa cctatacaaa
    12121 agagtgccat caggagtcac aatgcttgta aaggaaatga caaaatagga ggtaactatg
    12181 gataatagca atgaaaaact aaccaaaaaa gaatatcttg aattaatagt tgaaggcggt
    12241 attggagcaa ttccttatat tggtggcgtt gtgcagtcac tttattttgg gtctaaaaac
    12301 gagaagcgtt tcaaacgcat cgagaaattc tacaaaaatt taaacgaatc attagaaaac
    12361 attcgcgatc aaattccaga agatgttttc aacttagaga acaaggacca actcattgga
    12421 atattcgaag ggataaatga cgaggtagaa aaatcaaagg cacaaaataa aatagatatg
    12481 tataaaaatc tttataagaa ttgtttactt cgaattaaca acgcttcatg ggataacgag
    12541 gagtattatc ttcttgtact gaatcaatta acatcgattg agattcaatt gttagcatgc
    12601 ttaatgagtc gtggaaacga aaatttcact ggaaatatca gcatgtctgg ctattcacaa
    12661 gagctaatag atggtagctt aaatagatta tctgattttg gtctgttaga aaaaacgatt
    12721 agctccatag tattaggtga tgtcggcaaa caaaatatgg gctacaaaat atctcattta
    12781 ggaattcaat ttattaactc aactcttact taagttttct ttctaatatg acatctagac
    12841 ctatcagcaa tgcaacaata aaaaaccaga cataccaata atcaacaaat aatctaaact
    12901 ctggaagttc agttattctt ttaaaaatca ctttaatcat aataaacacc tctgattgga
    12961 tgatctaata cacatccaat tataccatta aaacgacaaa tagcacatcc ttgcgccggc
    13021 aagcaaaaag gatgtgcaaa aaataataaa acccaaacaa tgggcttctt tatagttcct
    13081 attgtatcag agaaagagag ctgattcaat tatggcaaga ttagtcaaac gtggaaatag
    13141 ttggcaatac gaaatttcat acaaaaaaga tgacggaaaa tacacgaaga taagaaaatc
    13201 aggatttaag acaaaaggag aagcaaaaga tgccgccaac gaattagaat ataacttgaa
    13261 caaaggcctt aaaggggatc gcaaaaatct attattatca gattactttg aggattggat
    13321 gcaactttat aaagaaggaa cagtatctcc tatcacttat agaaaatacg aagatacgtt
    13381 aatgaacata aagaaatata tgccagcggt attgatttct gatttagata gagtgggata
    13441 tcaacgcttt ttaaataagt atgctaaaga ccatgtaaaa tctaccgtta ttaagtttaa
    13501 taaccatatt agagcgtcgt tgaaagatgc cgtagaagaa ggattaattc cgtttgatcc
    13561 aactagaaaa gcagtaatca aaggaaaaga ttcattgaag ccaaaagaag ataaatattt
    13621 agattatgat caatttaaat ctttaatgaa actcgtagaa gaaaaccttt ctgcgcagta
    13681 ctcttctcct atgctcgtgt tagttgctgg tgccactgga atgcgatttg ctgaacttct
    13741 aggattaaca tgggaagata tcgatttcga agatcaaatc atcactatta ataaaacatg
    13801 gaattataaa ttaaatgaat ggggaaaaac aaaaaacgaa acttcaaata ggaaaatttc
    13861 cattgataaa catacgattg atctcttaaa aaagtttaaa atcaatcaaa aagaattatt
    13921 cgagaatttt gaaatcaaaa accctcataa ttttgttttt ttcaacttaa aaaatggatt
    13981 agtttcatca aacgccgtca gcaaatattt gcgcaaaaaa ttaaaagaat tagggattga
    14041 aaagcaattt actttgcatg gactaaggca tac
//
LOCUS       DAZUKC010000062        13969 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000062 DAZUKC010000000
VERSION     DAZUKC010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.063"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(325..2322)
                     /gene="tkt"
                     /locus_tag="ACG80T_001928"
     CDS             complement(325..2322)
                     /gene="tkt"
                     /locus_tag="ACG80T_001928"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFS4841563.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPIGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2424..2675)
                     /locus_tag="ACG80T_001929"
     CDS             complement(2424..2675)
                     /locus_tag="ACG80T_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFS4841564.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2822..3445
                     /gene="lexA"
                     /locus_tag="ACG80T_001930"
     CDS             2822..3445
                     /gene="lexA"
                     /locus_tag="ACG80T_001930"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFS4841565.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3624..3842
                     /locus_tag="ACG80T_001931"
     CDS             3624..3842
                     /locus_tag="ACG80T_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841566.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4130..4642)
                     /locus_tag="ACG80T_001932"
     CDS             complement(4130..4642)
                     /locus_tag="ACG80T_001932"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFS4841567.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4656..6953)
                     /gene="recJ"
                     /locus_tag="ACG80T_001933"
     CDS             complement(4656..6953)
                     /gene="recJ"
                     /locus_tag="ACG80T_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFS4841568.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLMHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPVE
                     FLDLVAIGTIADLVSLTDENRILVSLGINAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLANEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSN
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     IMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(7095..7538)
                     /locus_tag="ACG80T_001934"
     CDS             complement(7095..7538)
                     /locus_tag="ACG80T_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFS4841569.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7539..8327)
                     /locus_tag="ACG80T_001935"
     CDS             complement(7539..8327)
                     /locus_tag="ACG80T_001935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS4841570.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETDFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8324..9265)
                     /gene="rnz"
                     /locus_tag="ACG80T_001936"
     CDS             complement(8324..9265)
                     /gene="rnz"
                     /locus_tag="ACG80T_001936"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFS4841571.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9442..10029)
                     /locus_tag="ACG80T_001937"
     CDS             complement(9442..10029)
                     /locus_tag="ACG80T_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFS4841572.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10250..11563)
                     /gene="obgE"
                     /locus_tag="ACG80T_001938"
     CDS             complement(10250..11563)
                     /gene="obgE"
                     /locus_tag="ACG80T_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFS4841573.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11851..12861
                     /gene="gap"
                     /locus_tag="ACG80T_001939"
     CDS             11851..12861
                     /gene="gap"
                     /locus_tag="ACG80T_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFS4841574.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12946..13881)
                     /locus_tag="ACG80T_001940"
     CDS             complement(12946..13881)
                     /locus_tag="ACG80T_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4841575.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3856 a         3131 c         2454 g         4528 t
ORIGIN      
        1 gagcaatcgc ttttttattg atcaccattt ttcttttagg tctatggtgt cgggcactgc
       61 gggtcttcag ctcttgcatt caaattcctc ctcaaaataa aaaaacttaa taaaaagttc
      121 tcttcttaaa acaaacttaa ttaattaaat tatactttta attctcaaga aacacaaact
      181 aatccgaaga attctttgtt ttgtcattta aatgtaacat agccgaaaac gaaaagtaat
      241 attgatttat tcttctaaca cataaagaac agctgtctcc aacttgcaga cagctgttct
      301 tatcagtcaa acaattgctt ggatctatga tagttgattg tacgtattca cgacattttc
      361 tactgtaaat ccaaattctt ctaaaatttt agtgccaggg gcagatgcac cgaaatgatc
      421 gattgtaatc gttgcgccct ctgttcctac gtagcgttcc caaccaaatg atgcagcagc
      481 ttcgatagcc acacgtttct tcactgattt aggaagtaca ctttctttat attcagaaga
      541 ttgtttttca aagaggtcaa agcttggcat agaaacaaca gaaacatctt tgcctttttc
      601 agccaattct ttttgagctt tcacagccaa atcaacttca gaacctgttg caatcaaaat
      661 accttctggt gtttctcctt gtgatggaga aagaacatac gcgccttttt tcaccatttc
      721 atctgctact tcttttgtag aaggcaatac tggaaggttt tgacgactca atacaaggat
      781 agtaggcgca tctgttgtct ccatcgctac tttccaagca gcacgtgttt catttccgtc
      841 tgccggacga atcacttgaa caccaggcat gcagcgaaca gatgctagtt gttcaatcgg
      901 ttcgtgcgtt ggtccgtctt ctccgacagc aaccgagtca tgtgttaaga cgtaaacaac
      961 aggagtatgt tggattgctg ctaaacgaac tgctggtctt agataatcga caaagacaaa
     1021 gaatgttcca ccgtagatac gagttccgcc atgaagttgg ataccattca tagccgaagc
     1081 cattgcaaat tcgcggacac cgaaccagat gttgcgtcct tcataatgtt caggagtgaa
     1141 atctttatct gcagccacca ttgtattatt ggaacctgac aagtccgctg aaccacccca
     1201 gaagcttggt atagctttcg ataattcttg gatgacttct ttacttgaaa cacggcttgc
     1261 ttgactgctt ccaacttcat aagttggcaa ttctgcatcc cagttttctg gtagtttgcc
     1321 atcaaaagca tcttcaaatt gttgtgctaa ttcaggataa gcttttttat aattggcaaa
     1381 catttcattc catgcatctt cagctttttg tccttcttct atcatcgttt gatggaagcg
     1441 ggcagctact tcttctggaa cagtgaaatc tggatactcc caaccataaa cttctttagc
     1501 catcttgatg ccatcttcac caataggagc accatgaaca gcagatgttc cttctttagg
     1561 agcaccatag ccgatgactg tttttacttc gatcaatgtt ggtttgtctg tttcagcttt
     1621 tgcttcttca atcgctttag agattgcttc taggtcattt ccgtctttca ctaagatatg
     1681 ttgccagcca tatgcttcat agcgagcacc aacgttttct gtgaaagctt ttgaagttgg
     1741 accgtctagt gaaatatcgt ttgaatcgta taaaacgatc aatttcccta gtttcatatg
     1801 acctgccatc gaacttgctt cttgagatac cccttccatc aagtctccgt caccgcatat
     1861 tgcatacgta tagtgatcca tcacattaaa gttttctttg ttatacgttg ctgcaaggtg
     1921 cgcttctgcc attgccatac cgacagccat tgcaatccct tgtcctaatg gccctgtagt
     1981 ggcttctaca ccatctgtgt gatgtacttc aggatgtcct ggtgttttag aatcccattg
     2041 acggaattgt ttcaaatcat cgattgtcac ttgatagcca gctaaatgaa gcaagctata
     2101 caacattgct gacccatgtc ctgccgaaag aacgaaacgg tcgcggtcag cccagttttt
     2161 tgatgtcgtt ggatttactt tcaggtgttt tgtccataac gcatatgcca taggtgctgc
     2221 gcccatcggc aatccaggat gtcctgagtt tgctttttgg actgcttcaa tacttaatgt
     2281 acgaattgtg ttaacgccaa gttggtcaat tttgtcaaac aaaataaccc ctcctattaa
     2341 tttaggtttt ttttaccttt atttcttaca ctttcattct ataagatgtc acacgaaaaa
     2401 gcaatctttt tcagtatttt ttactaaaag ttgtcatttc ggttgtgtaa gccttttttc
     2461 ttttggattt cttttaattt ttccggggtc acgtcattgc cctcaggatc gacgactttc
     2521 attccttcga tatgatgacg cataccggaa cgaaaagctt ccaaatactc tttgcgtaag
     2581 ttgatttgtt cttgttcttc ggcttttgag agaccatttt ccttcttttt acgtgcaagt
     2641 tcattgatcc tctctatttt ttcaggtgac agcatgctca gccctccttt tactctcctt
     2701 catattatat aaaaactgaa aaaaaaacaa ctagataaac taaataaaat acgaacattc
     2761 gtttgattta gcgcttttta tatggtacta tttatttatc aagtgaaaga aggtgtacct
     2821 tgtggcccga caaactgaat caagacaaat cgaagtctta cgatatatcc atgaacaagt
     2881 aacccaaaaa ggatacccgc cgactgtccg tgaaatcggg gaagctgttc agctttcttc
     2941 tacttctact gttcacggtc atcttgcacg gcttgaaaaa aaagggttta tccaaagaga
     3001 tccgacgaaa cctcgtgcaa tcgagttgac aaaacaaggt ttagaaaaga ttgggattcg
     3061 tccaacaact attccagtac ttggcgtcgt aacagctgga gaacccattt tagcagtaga
     3121 ggatgcttca gacttctttc caatccctcc taatctttct tccgaagaag gcagtctctt
     3181 tatgctgaca attcgtggag atagtatgat caatgctggg atcttggatg gtgacaacgt
     3241 gatcgttcgg aagcaagaaa ctgctcaaaa tggcgatatt gtcattgcaa tgactgccga
     3301 ggacgaagcg acttgcaaac gattctataa agaaaaaaac tttttccgtc tgcagcctga
     3361 aaacgacttg ctcgaaccaa tcatcttaga tcaagtatct atattaggac gagtagtagg
     3421 actttatcgt agtcatatct attaattgct actaataaaa gaaaactttt taaaaagagt
     3481 ttgagacgat ggtctcaaac tcttttttcg cccgggttct tcttctttca aatttttatc
     3541 acttccctct ttacaaaccg aacaaatatt cgtatactaa atatacgaac aattgttcgc
     3601 ataaattaac gaggtgagaa ttcatgaaat caatgagacg tcttggctta gtattaactg
     3661 ttttgttttt aggtattatt attggtaatt tcggcatacg tccagcttta cttgtgtttg
     3721 ttccgttatt tactttgtgg ttcatgttgt gggatgaaaa gaagtaccgt caatctgagc
     3781 ggcgcagaat tcatgaagat catttctatc atgagcccta ctacagatcc aggcaatcat
     3841 aataaggatt gactgctaat tattcaaaag acaagaagtt aaacttttac gggggtgtga
     3901 caaaaatcgg tcacatcctt ttttcgaata aacggtgttc taaagccagt ttttcggtat
     3961 taagtcaaat tgcggaaaaa gtgatttttt aaacttctac tggtctttgc ttgtttccat
     4021 ttataaaaaa atagcgaccc tcaaaagtca gatagagaat ctgacttttg agggtcgctt
     4081 taaaatcgct tctttaagaa taacatcaag aatagcttct tttttctgct taatattcca
     4141 ttaatgtaat gatgtcatat cctttgattt tgtcgcgtcc atggagatcc atcaattcga
     4201 tcaaaaatgc gcagcctacg actacaccgc ctaattgttc gatcagttcg atcgtcgctt
     4261 tgattgttcc tccagtagct agcaaatcgt cacaaatcag cacacgttga cctggtttaa
     4321 tggcatcctt atgcaatgtc aatgtatctg ttccatattc taatccataa gtcacttcga
     4381 tggtttctcg aggtaacttc ccttttttgc ggactggggc aaaaccgact cctaactcat
     4441 aagcaacagg acatcctacg atgaatccac gggcttctgg cccaactacc atatcaattc
     4501 gtttttcttt tgcataattt acgatttgct tcgtagcttc ccgataagcg tcaccatctg
     4561 ccatcagtgg ggaaatatct cgaaacacaa ttccttttga cggatagtcg ggaatactgg
     4621 caatataatc ttttaaatcc actctttgtt tcctcctagg ttgttaacca tttctttatg
     4681 gtaggaatat cgctcaaaag caaaaattcc tcaactttga ttttattgag ccgccgctgg
     4741 tataatctgc tctcagtcaa aggatgactt tctgggctag cgactttttg taaaacaccg
     4801 ttctctattg taacaaattt caattcaaaa aacacctgta tcataaaaat taataatttt
     4861 tcagggatac gcaaatattg agctattgct tttattttat gacgtatatc aattcgttct
     4921 tgagaatgga tcagtttaaa caattttgcg tactgttctc ttgtgcctac cccattcaaa
     4981 taggcctcat caggcgaaat ccccatcaga taaacacgat tgaatgaata agaccgaaaa
     5041 acttctttca gatccgctaa atcatctgga caatccataa taactagaga gttcggtttt
     5101 cttttttcaa ttattgcctt tacagaatct agtccttcga aaacaatcgc agcttcctgg
     5161 ataatctcag gcacgaattt caacgtagaa gaatcaaatg ctaaataaag gctaccttcc
     5221 gacgttcggc tctgttcacg gaaacgtttt gcccgccaat caaatacttg cagaccaccc
     5281 acagaaaaat cagataccat cagctgcggt ttcttacgcc cgttccattc attgatcgaa
     5341 agctttccta ccacatctac ctgattattc agcagttctt cttcttgagt accaaaacca
     5401 aaagcgaccg catcaagctg actcgatgca ttggaaagga caaatttcag atgctgttgg
     5461 tttgctccga ttcttttgat attttctgct tggacttgtc gaaataaaaa ctgaggcaaa
     5521 ggattatccg taccaaatgg cgccaatagt ttcagctcat caattcgctc tactgttact
     5581 tcatttgcca gcaatacttc atcaattctc aactcaggcc ctctcatcaa gtcaatttga
     5641 tgatcgacta tatattggtt catcttctcc tgtagtatag tgacattttc acttggcatc
     5701 gttaaaccga ctgctgcatg atgtcctccg aagtaagtaa ataaatctcg catctggtca
     5761 agcatttcaa aaagattcaa ggcttcaata ctacgtccag atcctttagc tgatccatct
     5821 tcttttaaag taaggacaag cgttggttta cccgtttcat tcattatttt cccagcaaca
     5881 atgcccaaaa cgccttcatg ccatcctcgg tttaccaata gatggatatg attctcctta
     5941 ttgaccatcg ctaatgcttc ttgtgttatc tgctctacga ttgcttttct ttcttcattg
     6001 atctcattca atttttttgc ttgaacggat gcttcctctt catcaaacgt cgcaagtaac
     6061 gagacggcag gacgtggatc gcccatccga ccgattgcat ttagtctggg agcaatcgaa
     6121 aaaccgatcg ttgtttcgtc tgcgtccctc atttttacgc cgctttcttc aaatagcgcc
     6181 tgaagtccta tccgttctga atgatgaatg gcattgattc ctaatgaaac aaggattcga
     6241 ttctcatcag tcaatgaaac taaatcggcg atcgttccta tcgctactaa atccaaaaat
     6301 tctacaggcg gttcttccaa taatgcagaa gctactttga atgctactcc gactcctgcc
     6361 aaatctggaa atggatagtg ccccgctgga tgacgagggt gaacaatcgc ataagcgtct
     6421 ggtaaattct caggtagttc gtgatgatct gtaatgatca cgtctacacc agcttgctga
     6481 gcgtatgcta ccgcatcatt tcctgctacc ccgttatcaa cagtgatgat caactggatt
     6541 ccttcttcga tcttttcttg gtatacagat tggtttggtc catacccatg ctcaaatcga
     6601 tttggcagga atacttctac ttctgcacca agtaattcca gtgtttcttt cattaccgtt
     6661 gcactggtga tcccatctgc gtcataatca ccgtaaacga ggattttttc tccactgatt
     6721 acagcttctt ggattcggct gattgcttta tccatatcgt gcatcagata cggatcatgt
     6781 aattcttgtt cctttggatg taaaaatcgg ttgatatcct tttcctcatg gtaacctctt
     6841 tgccacagta acttaccgac aaatggagat aatttcagtt cacggatcat ttggagaaag
     6901 gattcagaag gctctgtctc gcttaacttc caatcaaaat ttgcttgtct cactttctca
     6961 ctcctaactt agttattata gcaaaggaaa tcagcaaaaa aaagcagaaa gaaagaataa
     7021 aaacagatca gatccgctag acagatgttc agcaggacct gatcttttgt acgaccattt
     7081 atgaaatatc cctattagtc aaaataattg aagctttgtt tgggcgattg agttttttgt
     7141 tcttccggcg ctttcaattg actgatttgt gcggttttcg cttgtaactc cgcctgataa
     7201 gcagcttcca actccgcacg ttcgtttcta aactcgcgtt gctgttcttc ttttgcttct
     7261 tcaattttct tttgaagttc tgtttgatat tctgacattt cctgagtcag ctgtttgatt
     7321 tgcttttttt gctgccatag tgttgccgaa gcagtcaaaa atacaatcag tgcaccaatc
     7381 agcgcagaac cgatgatcac taagatcaat ggcgcagaaa aggaagtgaa gccaaaactt
     7441 acaggaacat ttttattgtt caatacagca aaaagaacaa taatcaaaac taaaacaaat
     7501 ccaagaatca cattgctttg ctttttcaat tccttcacct actttttgtt aaaaattccg
     7561 ctggctaaat aatcaccaat cgttggaaac agcgtataaa attttgatgc aacttctaaa
     7621 ataaacggtt gattgatttc ccgttttgga tgtctcatat tgcggacgat catttttgcc
     7681 aacttggcag gttctaaaac gatttgtccg actttttcca aatagcttcc gcttggatca
     7741 gccttatcga agaaatctgt ctcgattgga cctggattca ctgtcgtaac agctacgcca
     7801 agtggtttca attctaatcg caaagcgttg gagaagccaa ggactgcaaa tttcgtggca
     7861 gaataaaccg ttgattttgc agttgccatt tttccagcca tagacgcgat attaatgata
     7921 tgaccttgtc ttctttctgc catatcgatc gcaaattttt gtgtcaaaac catcattcct
     7981 aagatgttga cttcaaacat atcgtatgct tttccaaggt cgaatgtaag gaagtcttcg
     8041 aaaatcccaa aacccgcatt gtttaccaga atatcgacgc gcccgacttc ttccatcacc
     8101 ttttcatata attcgtccac gctttcagga ttgctgacgt ctaccttgaa agcataagcc
     8161 tctttgccgc tcaagcgtct gcactcgtct ctgacacctt ctacaaatgc cattcttcgt
     8221 gcgcaactta taacgattgc tccttgcttt gctgcttcat aacagatttg tgcgcccaga
     8281 ccgcctgaac tacctgttac taaaacaact ttatcttcta aattcatgcc tcttcctcct
     8341 cgcgaaatgg aatctcaatg ctatcaaaat ctttcatgat cgtcgtgttt tcaaacactt
     8401 cttgtgcttc tttttcaagc tctaaagctg cttttcctaa atatctcgca ctgatatgag
     8461 taagtagcag ccgttttgcc tgcgcttctt ttgcaacttc agcagcttga tgagtcgtgg
     8521 aatggaaata gttgtgcgcc attcttgctt catctttatt aaatgtgctt tcatgtacca
     8581 gaatatcgct attttccgca agcacaacgg aattatgtgt ttttctcgta tcgcctaaaa
     8641 tagtcacgat tcgtcctttt ttatctggac ctacgaaatc ttttccattg atcgtccgcc
     8701 catcttctag ttggatcgtt tcaccttgct tcagtttgcc gtacagtggt ccagctggaa
     8761 tacctaattc ttttagacga tccacctgca attctccttt atgatcatgt tcaaccacac
     8821 gataaccaaa actgtcgatc ccatgattta gtttttttgt ataaacagaa aattgctgat
     8881 cagtaaaaat cggggctgtt tcatttagct caatgaattt cagcggataa cttaaacgcg
     8941 tttgcgaaat acctaaagag acttttatgt attcctctat cccttttggc ccgtaaatct
     9001 ctaatggcgt atccccacct tgaaatgacc ggctgctgat gagcccggga agtccaaaaa
     9061 tatgatcgcc gtgtaaatgt gtaataaaaa ttttaccgat ttttctcgga cgaatagtcg
     9121 ttcgcaaaat ctgcatttgc gtgccttcgc cacagtcgaa caaccaaact tcattgcgct
     9181 cgtctaaaag tttcaatgct atacttgtca catttctatg ttttgcagga acacctgcac
     9241 ctgttcctaa aaattgtaat tccataatct acctgacttt ctttaagttc ctctttaatt
     9301 ttagggtaaa aagaagggat aatcaaccgt aagtcgagaa tgggttcaaa ttaaaaataa
     9361 cgttctaaag cgatagcgtt ttctttgaaa attactataa aaacagcagc gaacccgttt
     9421 gagatcctct gctgtttttt attaatttga agatccagaa ctttcattgt cgttggaagt
     9481 ttgcggatgt ttgtccttcc aagtcttttg tgcagcttct ttcgcatgat tttcagcttc
     9541 tttcgcatca tgcacatctt tttcgacatc ttcataatcc aagcgggtaa tttctccgcc
     9601 taattccatc attaccaatt gattcagtgg gcgattgtcc tcttcatcag caatcaagat
     9661 cagtcctgta gctccttcac cgattttatt tcctacaaag tcgaacacgg tctgggcatc
     9721 ttttatttct ttggcatctt gtgtcgcacc gatcattcct ccggcaaacc atcctaataa
     9781 aatccctagc ggaccaccta atacaccaac caacattccg attaatccac ccgtagaaga
     9841 tttgttatta ccggtaaagt cgataaaatc ttctattttg aattgatgtg tcccattagg
     9901 atcatggctg acaactgcca tctgttctcc tttaatcgaa cgaacagcat gtaacttttt
     9961 gatttctgaa aatgcctgat atgcctggct ttctacttga aaatttaaaa tgattactcg
    10021 tttgctcatt aaaaacgccc ctttcatacc ttcttgttat ttattttacc tttaattgta
    10081 aaataagaaa agaaaagcac tctttttttc caataaaata gggctgtgac aaaagtcgta
    10141 tcacagctcc tttttccgaa taaacggcgt tcttgaagct gatcacttct gtcacaacct
    10201 cccttaaatc tgaatctata ttttttggta tttgttttta tttgcttgcc tattcgacaa
    10261 attcaaattc aaactcaccg atacgtacaa ggtcgccatc tttcgcaccg cgtgcccgaa
    10321 gagcttcatc cacacccata ccacgtaatt gacgagcaaa acgcatcact gtttcatcat
    10381 gatcgaaatt cgtcatttgg aataattttt cgatcttctc accagataag atccacgttg
    10441 catctgaatc acgatcgatt tggaactcag gaccttctgg attgaatcca tagtgtacag
    10501 tttcttcttc cagttcttct tcgtacaatg ggaattctgg cgttacttct aacaaatctg
    10561 ctgtagcatt caataacgcg tccaatcctt gtcgagtcac tccagaaatt ggaaatactg
    10621 ggatgtcatc agcaaattca tcttcttttt ccttgttcag ctgttctttg aatttcacta
    10681 gattttccgg tgcatccggc atatccattt tgtttgccac gatgatttgc ggacgctcta
    10741 gcaagcgtaa gttataagta gataattctt tgttgatggc aagatagtct tcataaggat
    10801 ctcgtccttc catcccactc atatcgatca catgtaaaat tactcgtgtt ctttcgatat
    10861 gacgaaggaa ttgtgtaccc agcccaacac cttgcgaagc tccttcaatc aagcctggca
    10921 agtctgctgc ggcaaaactt ctgccatcac ttgtagtaac cattcctaga ttagggacga
    10981 gcgtcgtgaa atgataagcg ccgatttttg gacgagcaga tgaaataacg gatagtaaag
    11041 tcgatttccc gacagatgga aaacctacaa gtccaacgtc tgccaatact ttcaattcta
    11101 gttcgatttt tcgctcttgt cctggttcgc cgttttcagc aatttccggt gcaggattcc
    11161 gaggagaggc aaaacggata ttccctcgtc caccgcgtcc accttttgcc acaactagtg
    11221 tttgaccatt ttcaattaaa tctcctaaaa gagcaccagt ctccgcatct cggacagtgg
    11281 ttccttgtgg tactttaaca taggtatctt ctgaaccacg accgtgcatc cctttactca
    11341 tcccattttc acctggctgc gctttgaaat ggcgattgaa ccgaaaatcc atcaatgtac
    11401 gtaacccttc atcgacgatc aagatgacat cgccgccacg accaccgtct ccgccagccg
    11461 gtccgccgtc aggaacgtat ttttcgcgac gaaatgcaac cattccgtct ccgcccttgc
    11521 cagctttcac atcgatcgtt acttgatcta aaaacatgga cataattaag tcctcctatt
    11581 attcatttat ttatttgact gcctgaattt ccacaataaa caaaagtctt acttatttta
    11641 tcggtgaaat ctgattttgt ctatcatttc agcaaaaaga ttgtttacct tcttcttttt
    11701 ttatcatttg gtaaggtttc caaccttttt tctgctacca agtataattt tttgacaaaa
    11761 tattctttgc gtagcagaat aacagtgtat taattgtatt aattgaggat gccgtttcac
    11821 agcaccccta ataaaggagg aacttttgat atgacagtaa aagtaggtat taacggattt
    11881 gggcgtattg gccgtctggc tttccgccgc atcaaagaag tctcagatga tattgaagta
    11941 gtagcaatca acgacttaac aagtccaact atgttagcac aattattgca atttgactct
    12001 actcatggta cttatcctgg cacagttact gcaacagaag actcaatcgt agtggatggc
    12061 gaagcaactc gtgtttatgc agaacccgac gcaagcaaaa tcccttgggc aaaagaaaac
    12121 ggtgtagata tcgtactaga atgtactggt ttttatacat ctgaagaaaa agcaaaagcg
    12181 catttagatg ctggtgtcaa acgagtagtt atttctgccc cagctggtgc tatgaaaacc
    12241 atcgtctata acgtcaatga tgatacatta gatgaaaacg acaaaatcat ctcagcaggt
    12301 tcatgtacaa caaactgttt agcacctatg gcttatttct taaataatga atttgggatc
    12361 gaagttggta caatgacaac agttcatgcc tacacttcca cacaaatgct cttagatgga
    12421 cctgtaaaag gcggtaattt gcgtgctgct cgttcagctg ctgacaatac tatcccacat
    12481 tctactggtg ctgcgaaagc aatcggctta gttatccctg agctacaagg aaaattacaa
    12541 ggacacgcac aacgtgttcc agtcgttgat ggttcattaa ctgaattggt ttcagtattg
    12601 aaaacaaaag taactgctga tcaagtaaac gaagcaatca aaaaacacac agttgacaat
    12661 ccttcattcg ggtatgatga ccgtcaaatc gtttctagcg atgtcattgg aacaactcaa
    12721 ggttcgatct ttgacccaac gcaaacagaa gtaacaactg ctggtgattt ccaacttgtt
    12781 aaaactgttg catggtatga caatgagtat ggatttactt gccaaatgat tcgcttacta
    12841 gaaaaattcg ctaacttgta atttgataga tcaatcgata aaagaagcct ttgactggat
    12901 ccagtcaaag gcttctttct tttcatcccc tacttttaaa ctcgttcaat gaacatattg
    12961 gcttaaaaat tgttggacta cttgctgata tttttcagga tctgtcttgt atgcttccgc
    13021 atgctcggct ccttttacaa catacttttc ttttggtccc ttcgttgcac gatagacctt
    13081 atctaacatt tcaaatggta caaatgtatc ttcgtctcca tgtataaaga gcattgggcg
    13141 ctggtttttc ttcaattgtt ccactgcatt tgcttcaccg aaaaagtagc cagcacggac
    13201 ttttgtcact agactcgtta cttgcataag cggaaatgct ggaagactaa acatatcgtc
    13261 tagctgataa gcaagctcat catgaacgga agaataaccg caatcttcaa tgattgcttt
    13321 cacattatct ggtagttttt ctccgctagt catcataact gttgctgctc ccatgctgac
    13381 accgtataga acgatttctt gcgattttcc attgttttct agcactttgt ttatccactg
    13441 tacatagtct ttacgctctg gccatccaaa tccaatgtaa tccccttcgc tttttccatg
    13501 tcctcttgca tcaggaacaa ggacattgta acccatatca tgaaacatct ttgcgtaatt
    13561 ggtcatcgtt tcagcgtttc ccatatagcc atgagccaca agagctgttt tttcactttt
    13621 cgtttcagca ggaagataaa tcgctgataa tttcagaccg tcttttgaag tgatggtcca
    13681 atcgcttcgg ttttcttttg aactaaacca tttatgtgca tccgtttctt tggatgtctg
    13741 tgttgtacca ggtgtatctt cgtctaaaaa gtctttctca gaaggcacaa cagcataatt
    13801 ataaaaataa ttccctgcaa acactaacgc aacaatcaga gctaaagcga cgcctaaaag
    13861 gattttcatc caaagtttca taaatcatcc atctcatttc tcttttttca tataaccttg
    13921 aattagtata atcaaagaat ctctactatg caatgttcat ttttataac
//
LOCUS       DAZUKC010000063        13810 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000063 DAZUKC010000000
VERSION     DAZUKC010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.064"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(221..1423)
                     /locus_tag="ACG80T_001941"
     CDS             complement(221..1423)
                     /locus_tag="ACG80T_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4841576.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1426..1620)
                     /locus_tag="ACG80T_001942"
     CDS             complement(1426..1620)
                     /locus_tag="ACG80T_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFS4841577.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1667..2515)
                     /locus_tag="ACG80T_001943"
     CDS             complement(1667..2515)
                     /locus_tag="ACG80T_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HFS4841578.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2720..3070)
                     /locus_tag="ACG80T_001944"
     CDS             complement(2720..3070)
                     /locus_tag="ACG80T_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841579.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3248..4111
                     /locus_tag="ACG80T_001945"
     CDS             3248..4111
                     /locus_tag="ACG80T_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4841580.1"
                     /translation="MFGLVQPNKVKVGQRIKEIKDGMSLSFTELGNRLGLKKPTISSY
                     VQGYALAPKSVINQLSSISGKPVGWFYFGHVEEYIREYLFLIGQKKIVDDYPEIIEKI
                     KQEFYTGEFKNPAWENELGYPLEEFIQECFVEYSSEIMKDYVVQITKEVLEESKTLNK
                     LSSKAKEEAVTILSSGIIDYIEMSGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSY
                     LIGKLINILGTDEDTELFLSSLSMIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREK
                     TPDEFYDWFEK"
     gene            4735..5727
                     /locus_tag="ACG80T_001946"
     CDS             4735..5727
                     /locus_tag="ACG80T_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4841581.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5743..6627
                     /locus_tag="ACG80T_001947"
     CDS             5743..6627
                     /locus_tag="ACG80T_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4841582.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6676..8283
                     /locus_tag="ACG80T_001948"
     CDS             6676..8283
                     /locus_tag="ACG80T_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4841583.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8284..9768
                     /locus_tag="ACG80T_001949"
     CDS             8284..9768
                     /locus_tag="ACG80T_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFS4841584.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9945..10937
                     /locus_tag="ACG80T_001950"
     CDS             9945..10937
                     /locus_tag="ACG80T_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4841585.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10989..11858
                     /locus_tag="ACG80T_001951"
     CDS             10989..11858
                     /locus_tag="ACG80T_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS4841586.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12278..>13810)
                     /gene="guaA"
                     /locus_tag="ACG80T_001952"
     CDS             complement(12278..>13810)
                     /gene="guaA"
                     /locus_tag="ACG80T_001952"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFS4841587.1"
                     /translation="KIIVLDYGSQYNQLITRRIREFGVFSELMSHRITAEEVKAMNPK
                     GIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGK
                     AELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKM
                     YGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLG
                     LSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAK
                     ERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGT
                     ETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPG
                     LGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRT
                     YDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVE
                     WE"
BASE COUNT         4231 a         2337 c         2851 g         4391 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttacgtt gaaaaacgtt gatttatcgg cattccccat ccatattata
      121 tttacgtctt attcccactc aatatgagta gcctcgtaaa gaagctatta tatcagtgct
      181 ttttcagcct attcctcatt ttggtaggga aatggtaggg ttagattttt actgcttgat
      241 ttccattaaa gtttgcaact ttagattttg ttgcacaggt aaatttaatg tttcctgtca
      301 tttgttctgc aattccttca tcattacggc tccatagatg agcgtagtgc tttaaagtga
      361 tttcagggct agaatggcct aaccgttgcg acacgaccag aatgtctgcg ttaaactcat
      421 tgattaggta gctaacatga gaatgacgta atccttttcc ttgtattagg ggtaccttag
      481 ctagtttggc ataacgctga ataattcgtt gaacagttga acgatagagt gggaggtcag
      541 tgtagctaag aataaattgt tctacaccat gatttttttg aataactttc cactttttta
      601 gcacttcaat tgtatcatcg tccagagaaa tgatccgcaa gccgctttca gtttttgtgt
      661 aaggtttccg aataaaatct ttttgatttg tcatttccaa ggtgtgatga accctcaact
      721 ttttacgttt gaagtccacg tcattccaat taagtgctaa tccttcgctt acacgaacac
      781 cagtcatgta atatagccag atcattaaaa aacacatatg ttcgtagaaa tcatctttat
      841 aaatttttgc caaaacttct tcaaattctt cttttgtcca gtaaggaacg attgattttc
      901 cttttggaat tgcttttgtt cttttggaaa tattctcatt cagaaattgc agggtgaccg
      961 catagtctaa tgatttacga aacataccgt acataagtga actgtatgct tgtgagtatc
     1021 cgctatcgtt caataaccaa attcgataat tttcacaatc tcgcacattg atttcacgaa
     1081 gcttcttatc accgaaaaat tcagtaatct gaatcaatcc acactcacga gaactccaag
     1141 tacttttttg tacgctagct ttgtagtgtg gaacgtaaga ctgctccata aattctttgt
     1201 aagttaaatc ataattcgca aatccattta caaccatgta ttcatttttt atacgcatga
     1261 cttctttgtg tgcttcacga gctgatgaga atcgttttcc tagagaatct tttgttcctt
     1321 tcttctggat cctctttcca gtcacttgat ctgttcctaa ctcaactaga taagaaaagc
     1381 gaccattttt atcttgatac acacaagggt attttgtttt catagttatt cctcctgtga
     1441 aagaggaaca cctataattt cttcaattac ttgtcggggt actaccccaa ctcttttatt
     1501 attataatat ggatatccct gatttaccat tcttgttttt gcttgtcgga ttattgtggt
     1561 agcttgatgt gttttaaatc ctagagcctc taaatctttt cgacaaattg tatttttcat
     1621 tgtataaact cactttcaaa gaaaacacta tcattgggta atagtgttat ttctttattt
     1681 ttgtataatg aacggtactt tcttgaaaat ccatcacttg ttcaggactt cccaactcat
     1741 ttaaagctaa agcattgtaa aaaaatggac gtctaactat atattcgctt gtatatagta
     1801 tatcttgatt cgtggaatca tagggtttct ttaccatctt tttggtgaca gttggtagtt
     1861 tgagattccg ggacttgaaa aaagcttttt tcttatgctc tttcaattcc agctctttat
     1921 cgaaatattt gctgatatag cgcccgcgat tttcgatact gtctacatcg atcttgttga
     1981 tcttaacgaa accctgaccc caaatcgtag ttaaccggtt aaatgagata aaggggaaat
     2041 caaacaagat aatatggtag tggattgcac cgcgttgctg cttttcccac gtggcgagat
     2101 actttaaatt tgctttttta gttttataga ggtcataatt cagtcgtttg ataaattttt
     2161 taaattcatt atttgcatac tcaatgtctt gaatgttttc tttgaaagtg agagttaaaa
     2221 atttcgtttg attgtcaaaa ttcgtgtcta caaggcgggc aatctcaaat cgcatacttt
     2281 gataatgttt ctgtttgcgt tttaaactgt catattgtcc ttgagcggat aattcatcat
     2341 agttttttct tttttcaaga gtaatatcat tttcataaat ccaagctaat ttttgttttc
     2401 gttccagatt tacttcgttt tcgatcgttt tacgtttggg agtaatgatt gcttcataat
     2461 cataaatttc aatatacgtg ggagtttcaa tgattttctg attataagct tgcaaaaatc
     2521 atcactcctt gttgcgtatt cgattgtcaa agaaccagtc attttctcct gatgttgatc
     2581 tataaatcaa gttaaggggg aaggcctgcg tagcagccct tctagcctat ggggtgttgg
     2641 gcggtcttaa cggacagaaa cggctctgtg ggcgctgccc cgccgtttcg tcctaaccac
     2701 ccaacactcc atagtatagt tagttttgtg tagtggtacc ttgttttctg ggaatcaaag
     2761 ttgccgtatc agcggttgcc gataacccat aactgacaaa ttgtccattc gagcgtcccc
     2821 aaggtacaat caccaaattg gtaaatgtaa ttatggctaa accttgttgg gtaagttctt
     2881 ccggcgtgat cacaggatgt tcagatttga ctttgacact gattttttcg tagcgaagtt
     2941 ttggtaggat acattcgtag ctccaaccta gaatttcgcc ctccttttgc cagcgagtga
     3001 cattgacgac ttggtattct ttttcgagtg tttcttttgg taaactgaga tcggtaattt
     3061 ttattggcat cacaattctc cttttttata cgaaagttta tacgatttga ttaaaaaata
     3121 tagatggcca tctacgactt gtattatata cgagaactta tacgtagtca agagattttc
     3181 taaaaaaata tatacgattt tttatacgag tgttataatg aaaaaaatga taaggaggaa
     3241 tttgaatatg tttggtttag tacaacctaa taaagtgaag gtgggccaac gaatcaaaga
     3301 aataaaagac ggaatgtctc tctcttttac agaattaggg aatcgattag gtttaaaaaa
     3361 gccgacgatc agttcctacg ttcaaggcta tgcgttggct cctaagagtg tgatcaacca
     3421 attatctagt atcagcggaa aacctgtcgg ctggttttat tttgggcatg tggaagaata
     3481 tatccgagag tatttgtttc tcattggtca gaaaaaaata gtggacgatt atcctgaaat
     3541 cattgagaaa ataaagcaag aattttatac gggcgagttc aaaaatcctg cttgggagaa
     3601 tgagctaggc tatcctttgg aggaatttat ccaagaatgt tttgtagaat attcgagtga
     3661 aatcatgaag gactatgtcg tacagattac gaaagaggta cttgaagaat ccaaaacgct
     3721 aaacaaacta tcatcaaaag caaaagaaga agctgtcacg attctttcat ctgggatcat
     3781 tgactatatc gagatgagtg gtgagatcaa gtatggagag aaagatcgca ttgcgaaaat
     3841 ggtgaaagaa tcaataaaaa cctttgatgt gaaacaagac ctgacattta atgattccta
     3901 tttgattgga aagttgatca atattcttgg tacagatgaa gatacggagc tgtttctttc
     3961 ttctttatca atgatattta ctgataagca tttttccact cgatttggtg gggaagaatt
     4021 agtcgaaata tttcaatcga tgcgaccagc gttaatcaaa ctctatcggg agaaaacacc
     4081 agatgaattt tatgattggt ttgagaagta aaataagaaa agtggcttag attacaagtt
     4141 ggtgaaaaaa tttttttcag cataacatca gtaatctata aaggggttgt tgattatact
     4201 gtagaagtta agtactcaag agattttttg tacgtaaaca aagcaaaaaa gggtgcagtt
     4261 tcactagaaa atgtactctt ttttgtgatt tttcttagtt atagagtttt tttgaaactt
     4321 taaaaatgag aaaacatatt gattttaaag agtgtagaca tagcttcttt tgctaaatag
     4381 attaaactta ctggtgagtg gtcttgaaca tacttttaaa tcacatttta ctgagtttta
     4441 ttggatttta taattcattg gaaacctcgt acttaatctg tttttatcta tcgactagaa
     4501 tctgtatata ttccagctat caatgaggcc gcagaaaagg gtgcttaaaa ataatctgtt
     4561 gtttaaaata ttattatcag aatacttaga tacaaaattc tggcacaaag taaaaaaaat
     4621 attgacttaa aaaaatggat atggtaatct ataaacgaaa atagtggtaa cgtttccaaa
     4681 atgcatcttc gttaaatttt tattttttta aacgaaaatg gaaacgtttc taaaatgtat
     4741 tgggaggaga ttgaattgga aaagacattg ggaccaggtg gtgagataag ggcggtacaa
     4801 cgggataaaa aaaatatttc gaagtgggaa aaattaaaga aacagttgcg gcgtgataag
     4861 tggatgtact tgttgctctt accaggtatc atcatgacgt ttatctttaa gtatgtgcca
     4921 atgtacggtg ctgtgattgc atttaaagac tataatccta tgctaggaat tttggaaagt
     4981 ccttgggtag gactagatca ctttagggaa tttatcagtt caccaaattt tacgcttttg
     5041 ctgagcaaca cattaaaaat tagtatttat ggtttattgt taggtttttt tccaccaatt
     5101 attttagcac tgagcttgaa tcaaattatc aataaaaaaa taaaacagaa aatccagttg
     5161 attctatatg cgccgaattt tatctcagta gttattattg tggggatgct gtttttgttc
     5221 ctatccgcaa atggacctat taatgggtta gtagaaaagg ttacggggca accaattatg
     5281 ttcatgtcta atccagctta tttcagaacg atttatattt tatcgggaat ttggcaagga
     5341 atcggctggt catccattct ttacaccgca acgctggcgg gagtcagtac cgatttatat
     5401 gaggcagcaa atatcgatgg ggcaaatatt ttacaaaaga tttggcatat cgatttgcct
     5461 gcattgaggc ctgtgatggt gatcagcttc atcttggccg ccggcggtat tatgaatgta
     5521 ggatatgaaa aagcctactt gatgcaaact tcaatgaaca tccctgcctc agaaatcatt
     5581 tccacttatg tatacaaagt cggcttgcaa tcaggtgatt atgcctactc tgctgctgtt
     5641 ggtctgttca atatgattat taatgtcgtt ttattactga ttgtcaacaa tataaccaag
     5701 cggctaaacg atggtcaagg cttgtaatga aaggagaaaa ttatgaatag tgctttatca
     5761 aaaatcgacc gtcaaatctt atggttgaat cgagtgattg taactttttt agtcgtcatc
     5821 accattgttc cgctgattta tgtactggta gcttctttta tggacccagc tactttgatc
     5881 aataaaggta taagcttcaa tccgaaagat tggacaatac agggatatca acgggtattt
     5941 gcagatgatt ccattttacg cggttttgta aattcgttgt tttactcttt tagcttcagc
     6001 ttattgactg tatttattac gatgatcact gcgtatcctt tgtctcgacc taacttggcg
     6061 gggaaaaaga ttattatgac tttttttgtc attacaatgt tcgtgggtgg tggcttgata
     6121 cctacctatt tattggtaaa gaacttgggc atgttagata gtgtatgggc gattatcgta
     6181 ccaggtgcta taaatgtgtg gaatattatc ttagcccgta cctattttag tgctttgcca
     6241 aaagaacttt ctgaagctgc tactatcgat ggcgccaatg acttgcaaat actgttcaaa
     6301 gtcatgcttc cattagcgaa accaattatg ttcgtattgt ttttatatgc ctttgttggg
     6361 caatggaact catactttga tgccatgatt tacatcaaag atcccgactt acagccgttg
     6421 caactcgtat taagaaatat tttgatccaa aaccaacctt cacaagacat ggtgggagcc
     6481 aatactgcca tggcagaaat gaaacaaatt gctgagttga tcaaatacgc aacgatcgtc
     6541 atttctagtc tgccattaat cataatgtat cctttcttcc aaaaatattt tgataaaggt
     6601 gttttagttg gttctgttaa agggtaagta ttgttcacta tttgtatgaa taaaaattat
     6661 aaaggagatt ttaagatgaa agcaaaatgg atgctgccaa tcgtttttac ttccgtacta
     6721 attttggggg cttgtggagg ctcaaaagaa aagagtaaat caagccctga ttatgaacta
     6781 gaaaatgtct cttttccatt gaaagaaaaa gtatctttaa aaatgatgtc tcaaagtgcc
     6841 cctttggctc ccaatgatcc taatgacaaa ttgattttcc aacgattaga agaagagacc
     6901 aatctccata ttgattggac aaactacagt agtgattttg ccgaaaaaag aaatttggat
     6961 atcgctagtg gtgatttacc agatgccatc ttcaacgccg ctgcaggtga ctatgattta
     7021 ttaaactggg cggaaagtgg cgttattgta ccgattgaag atttgatcga agattatatg
     7081 ccaaatctga agaaaatttt tgatgaaaac cccgaatacc gacaaatgtc tactgcacct
     7141 gatggtcaca tttactctat gccttggatt gaagaattag gcgaaggaaa agagtcgatt
     7201 catacagtaa atgggatggc ttggatcaac aaggagtggt tggataattt gggtcttgaa
     7261 atgccgcaaa ccactgatca gttgatgact gtgttggagg ctttcaagac gcaagatcca
     7321 aatggtaatg gtgaagcaga tgaaattcct tttagcttta ttaacaatgg aggaaacgaa
     7381 gacttaaaat ttttaatggg ggcattcggt ctaggtgata atgatgacca cttagtcgtt
     7441 gataacgatg gtcaggtaca atttactgct aacaaagaag ggtataaaga agccatcaaa
     7501 tatttcaatg aaatgaataa taaaggtctt attgataaag aggctttcga acaagattgg
     7561 aatacgtatg ttgccaaagg gaaagaacat aagtatggcg tgtactttac ttgggataaa
     7621 gcaaatatca cgggtgcaga tgatagttat gacgtcttgc cagtcttagc gggaccagat
     7681 ggaacgaaac atgttactcg tacgaatggc atgggtttct caagagatcg tttcgtaatt
     7741 actagtgcga ataaaaactt ggaacttaca gctaggtgga tagataaaat gtatgaaccg
     7801 ttacagtctg tccaaaataa ctggggaacc tacggtgatg aagcacaggc gaatattttt
     7861 gaattaaagg atggtatgtt aaaacatttg cctttagagg ggacagcacc gggggaacta
     7921 agacaaaaaa ccgaagctgg aggaccatta gcagttcttg attcttatta cggcacagtc
     7981 actactatgc cagatgatgc gaagtggcgt ttagatttga tgcatgacac gtatttgcca
     8041 tatattacag aagacaatat ctatccacgt gtctttttag aaaaagaaga aacagatcga
     8101 ttagcaaaaa tcgaaacgga tatgtgggat tacatctatc gcaaaagagc agagtggatc
     8161 accggcggta aaatcgatga agaatgggaa gaataccttg ctgaattgga acgtgtaggt
     8221 ttggaagaat ggattcaaat aaaacaaact ggctatgata attttttgaa gggagcacaa
     8281 taagtgttag tagaagaaac ggcgattttg gataaagcaa atcgatttat tgaaatgatg
     8341 cgaggaaaag ttaaccctca gtatcgacaa aactatcatt tttccgcacc catcgggtgg
     8401 ataaatgacc ccaatgggtt tatatattac aaagggcatt accatctctt ctatcaatac
     8461 tatccttacg atagtcgatg ggggcccatg cattgggggc atgcgcgcag tagtgatttg
     8521 gtccattggg aagatctacc agtagcgtta gccccagatc aaccttatga cagcggcggg
     8581 tgtttctcag gaagtgcaat tgaaagagac ggaaaactat atttatttta tacaggctgt
     8641 caagaaattg atggtattag ccatcagcat caatgtttgg cggtctctga agatggtatt
     8701 cattttagca aatacagtgg taatcctgtg gtagcagaaa aacagctagg tgaaaatggg
     8761 ctaatacaag aatttcgtga tcctaaagta attgttcaca acgatggtta ctatatggta
     8821 gtagctacta aatctacaca gtctactggt agaatagtga tgtttcaatc gtcagatttg
     8881 tatacttggc acttttactc tgtgctgcta gaagggactt cagagcaagg aataatgtgg
     8941 gaatgtcctg atttattcca tttagacgga cgagatgtct taatcatgtc tccgatacaa
     9001 atcaagaaga acggactgga atatcataat atcagttcta ccatggcttg tattggtgag
     9061 gtaaactggg aaacaggtca gatgatagtt gataatttcc atgaaatgga ttttggaatg
     9121 gatttttatg caccacaaac aacaagagat cctaatggtg gacgggtgat gattgcttgg
     9181 atgcagatgt gggatcgtac attgcctaca catgagttag gccatctttg ggcaggctct
     9241 atgagtttgc cccgagagtt aagagtgaaa aaaaatcgat tggtacaaaa acccatctca
     9301 actgtatata gtcatttagt ttatcaacaa ggcagtgaaa atattgcagt gactgacaca
     9361 tcagtcgtat ttcgaaacgc gctgtctgat tcttcatatt tacatctagt cgcagatttg
     9421 gagaaagctg aagtttttac aattgaatta ctgcggtcta agacggattg tctcaaacta
     9481 gcctatgaca ctgctacaca ggtttttgaa ttggatcgtc agaaactagg aagagagctt
     9541 acaggggcgg aagaagaccc tctgaatgta cgaaaagttg aagtaccttt gattgataat
     9601 cagctcgttc ttgagatttt tagagatacc tcatcaattg aagtctttat taatgggtta
     9661 gaagttatga cggctacttt ctatgagctg gaaaaagctc gagatatctt gtttcattcg
     9721 atcggagaaa gtcgtattca agcttttgaa acagcgagtg tgaaataaat gtttgattgc
     9781 ttaggcgatg aatcaattat ttaataggtt ttgaaggata atgtttttag aaaagaacag
     9841 atagctagat ttcccatgga tttgctatta gtattttgaa aaaattagta atttatgaca
     9901 aaattcatta tactaaactt gtgcagaaat gtgagggatc aactatgaaa gcaacaatga
     9961 aagatgtcgc aaaattagca ggtgttggag taggaactgt ttcccgggta ttgaataacg
    10021 gttctgttaa agcttctact agagaaaaag ttgaagcagc aatgacagag ttaaactatc
    10081 aaccagattt ttatgcccgg gggttaaaga tgaagcatac gaacacggtt gctttgattc
    10141 tacccacggt atggcatcca ttttttggcg aatttgccta ccatgtagaa acagcattag
    10201 aaagacaaaa ctacaagctt tatttgtgta actccagtgg taatagtgaa aaagaacaag
    10261 aatatatcca gatggtcagc caaaataaag tagacggcat cattggaatt acttattcag
    10321 atatcgatga atacatttcg agtaatctgc cttttgttag tattgaccgt tatttcactg
    10381 aagaggtgat ttatgtgacc gctgacaatg cggccggtgg acgtttggct gcaaatgaac
    10441 tttatcgccg aggatgtcag aatgtagcat atatcggtgg gtaccaagaa acaccgaatg
    10501 aaacgaagag tcgtcgaaaa tattttgaaa tcgagtgccg tcaactgggg atttcctata
    10561 aagcgttaga tatgttagaa cctttagacc atctctcaga acgtgtaaca aattttttgt
    10621 tagagaatcc cgatatcgat gggattttta cgatcaacga tccaatggca ttagctgtca
    10681 ttgatatgct tgaaaaaata gggaaaaacg taccagagga tgtccaagtt atcggatttg
    10741 atggtcaaaa gatgacagaa ggagatcctt atctagtctc aacgattgcc caacctacaa
    10801 agcaaatggc ggaagtggca gtggcaaaat taattgctgt cattaatgga gagacagtag
    10861 aaaaacgtac cgtattgccg attgaatttg gcgaaggtga cacaacgaaa cctttagtga
    10921 gaaacgattt tgcttagtgc aaatacgttt ctttttttga aaatgagtga atgaaaggtg
    10981 tagataaaat gttatatggt ggaatagaag ccggtggaac aaagtttgtc tgtgggattg
    11041 gtaatgaaca attacaagtg atagaacgga aaagttttcc taccacaaca ccagaagaaa
    11101 cgatggagca agtgtttgat ttctttgata agtacaaagg aaatatcgca tcaattggtg
    11161 taggagcatt tgggcctatt gatattcaaa tcgaatcaag aacttacgga tatatcacca
    11221 acacaccgaa gttagcttgg caaaattatc attttgtagg agctttggaa aacgccttgt
    11281 cggttcccat ctattggact accgatgtaa atgctgcttg ttatggagaa tatatggcag
    11341 ggtatggaaa aggtaaaaat agtgttctgt attacaccgt tggtacaggt gttggtggtg
    11401 gtgctgtagt ggatgggaaa atagtcgcag gttttagtca tcctgaaatg ggacatatgc
    11461 tcttacaacg tcataatgaa gatcaatttg aaggaatctg tccttttcat catgattgtt
    11521 tggaagggtt agcatcagga cctgcgattg aaaaacgctt tggaagaaaa gcccagacta
    11581 tctcatttga tcatccttat tggacgattg aggcagatta tcttgctcag tgtgtgtata
    11641 acacaacgtt actatatgca ccggaagtaa taattttagg cgggggtgta atgaaacagc
    11701 cgcaactact cgagttgatt cgccaatcgt ttaaagaaaa aatgggttcc tacgtggata
    11761 cgcctactct cgaagaatat ctactaactc caaggcttgc tgacaatgcg ggcttgattg
    11821 gctgcctagc tttagcaata aagaaactca atcattagaa tagtatttat tagaactatt
    11881 cataacatac cggaagttta aaagggtatt ctgaaactta aatagtctca attcattctt
    11941 ctttcttgag aatctgagta gagactttta ttctgtttca aattaatata attacgaaat
    12001 gatattgagt acaagaaagt tctttttacc agatctttta aatcgaaaaa tctgatatat
    12061 atatttatag tactaaaaat ctgattagat aaaaagctaa ttcaatagga aaataaaggt
    12121 agtgaataaa agcaaagccc ttgttagtag ttaatctaac aagggctttg gtagggaaaa
    12181 tggtaggaaa ccatcttatt ttttacgaaa atcaggaact ttagtaattg aataatgttg
    12241 atttataggt attcctcatc catattatgt tggctcttta ttcccactcc actgttgcag
    12301 gtggtttgct ggtaatatca tacacgatac ggttaacgtg tgcgacttca tttacgatac
    12361 ggacactgat tttttgcaag acttcccaag ggatacgggc aaagtcagcg gtcatgccgt
    12421 caatagaagt gacagcacgg atacctacag tgtagtcata cgtacggcca tctcccataa
    12481 ccccaacaga gcgaatgcct ggtaggacgg tgaagtattg ccagatgtcg cgatctaaac
    12541 cggcattggc gatttcttcg cgcaagatag cgtctgattc acggacgatt tctaattttt
    12601 cttctgtcaa ttcaccaagg acacgaatac ctaagcctgg tccagggaat ggttggcgcc
    12661 aaacgatgct gtcaggcatg cctaattggg tacctaattc acgtacttcg tctttgaaca
    12721 atgtgttcaa tggctcgatc agcttgaatt gcatgtcttc aggtaatcca cctacattgt
    12781 gatgtgattt gatggtttgg gcagtttcag ttcctgattc gatcacatcg gtataaaggg
    12841 ttccttgtgc aaggaaagaa acgccttctt caccggcaag tttggttgct tcatcatcga
    12901 agacatatac aaattcattt ccaatgattt tacgtttttc ttctggatca gacacgccag
    12961 caagttttga taagaaacgt tctttggcat cgacttttat aatgttcaag ccaaattttc
    13021 cgccaagact ttccataact tgttctgctt cacctttacg caacaatacg tgatcaacga
    13081 agatacatgt taattgatcg ccgatcgctt tttgtaaaag aacaccaaca acagaggaat
    13141 ctactccgcc tgaaaggcct aataaaactt ttttgtcacc gacttgttca cgaatttttg
    13201 cgatctccat gtcgatgaag ttatccattg tccagtcacc agtacattgg cagacatcta
    13261 gtgtaaaatg gcgcaacaag tcatttccat attcggaatg gcgaacttct gcatggaatt
    13321 ggattccgta cattttgcgt tctgcatttt ggatcgatgc gatggggcaa tctttgctgg
    13381 tagcaacgac atcgaaatca gtcggtgctt gggtaactag gtcaccatga ctcatccaaa
    13441 cggtttgttt ttcaggagtg ttttggaaaa gggccgcttc tgcagaagtg atttccaatt
    13501 ctgcttttcc gtattcacgg tttttggctg gtttaacttt tccgcctaat ttataagtaa
    13561 tcaactgcat accgtaacag attcctaaaa ctgggatccc caaatcaaaa atcttttcat
    13621 caataccaaa ggccttctca tcataaacac tgtttggtcc gccagaaagg atgattcctt
    13681 tagggttcat tgcctttact tcttctgcgg taatgcggtg gctcatcaac tctgagaaaa
    13741 caccgaactc acgaatacgg cgagtgatca gttggttata ctgactaccg taatcaagta
    13801 cgataatttt
//
LOCUS       DAZUKC010000064        13531 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000064 DAZUKC010000000
VERSION     DAZUKC010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.065"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(164..388)
                     /locus_tag="ACG80T_001953"
     CDS             complement(164..388)
                     /locus_tag="ACG80T_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFS4841588.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(586..2166)
                     /locus_tag="ACG80T_001954"
     CDS             complement(586..2166)
                     /locus_tag="ACG80T_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFS4841589.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2567..3943)
                     /locus_tag="ACG80T_001955"
     CDS             complement(2567..3943)
                     /locus_tag="ACG80T_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFS4841590.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(4071..5159)
                     /locus_tag="ACG80T_001956"
     CDS             complement(4071..5159)
                     /locus_tag="ACG80T_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS4841591.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5331..5765
                     /locus_tag="ACG80T_001957"
     CDS             5331..5765
                     /locus_tag="ACG80T_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4841592.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5843..6376)
                     /locus_tag="ACG80T_001958"
     CDS             complement(5843..6376)
                     /locus_tag="ACG80T_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFS4841593.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="ACG80T_001959"
     CDS             complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="ACG80T_001959"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFS4841594.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7641..8438)
                     /gene="recX"
                     /locus_tag="ACG80T_001960"
     CDS             complement(7641..8438)
                     /gene="recX"
                     /locus_tag="ACG80T_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFS4841595.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8545..9927
                     /gene="rlmD"
                     /locus_tag="ACG80T_001961"
     CDS             8545..9927
                     /gene="rlmD"
                     /locus_tag="ACG80T_001961"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFS4841596.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10101..10679)
                     /locus_tag="ACG80T_001962"
     CDS             complement(10101..10679)
                     /locus_tag="ACG80T_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFS4841597.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            11001..11471
                     /locus_tag="ACG80T_001963"
     CDS             11001..11471
                     /locus_tag="ACG80T_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFS4841598.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11579..12805)
                     /locus_tag="ACG80T_001964"
     CDS             complement(11579..12805)
                     /locus_tag="ACG80T_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS4841599.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         3981 a         2728 c         2251 g         4571 t
ORIGIN      
        1 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca gaagcaactc
       61 cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa atttttgtga attccagctt
      121 atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt tttgattttt
      181 ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct ttatttttaa
      241 ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg tttttgtccc
      301 agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc tgtttttttc
      361 tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta atcatatgta
      421 cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat atagtaaaaa
      481 gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg tttttgtttg
      541 gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca tcaaactttt
      601 taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt tctcgaataa
      661 gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac tcatcttctc
      721 atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag acatgattac
      781 ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta gctgaccaac
      841 agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg ctccttcttg
      901 aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt ttgcagtcac
      961 tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt ttagttttcc
     1021 ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga tgatatcgcc
     1081 tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga cattgcttaa
     1141 tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga atgttccaga
     1201 acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg cttggatctt
     1261 gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat cttgtgtttt
     1321 gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct gtacaccgaa
     1381 gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa ttttttcttg
     1441 attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt gttcatataa
     1501 ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca attctactaa
     1561 tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt agatatcata
     1621 aagaccttct agccctttac ccataccgat tggccaattc atcggataag attcgatatc
     1681 taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac gatctagttt
     1741 gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata atttctttgt
     1801 ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca cggccatcaa
     1861 agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga tatttactcg
     1921 tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac gttgtttctc
     1981 gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta cggtcccggc
     2041 ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt tcccagcatc
     2101 cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta attttggatt
     2161 attcataacc taactccatt tcttccattc aattatttat attttctcta ggcacagatt
     2221 gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc gtgtagcagt
     2281 ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc ccacccaagc
     2341 caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca ggatagcttt
     2401 ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca gcgaatgaaa
     2461 aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag aaaagacgtg
     2521 ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat cttcttggct
     2581 cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc gcttctgttt
     2641 cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc atttcttcag
     2701 aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc ggaatcatgc
     2761 ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc agatcactgt
     2821 caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat tcgtgttcat
     2881 cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct ccgacgattt
     2941 cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca tctaataaaa
     3001 tagccatctg attctgcgtc tttttcattt catataacag atcatcaata aagaccgttt
     3061 caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca aagccgaatt
     3121 tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta tctttgtctt
     3181 cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca ttttcttcca
     3241 cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact tctcgggcaa
     3301 ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta ttcaatacgc
     3361 cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct tcatcatcaa
     3421 aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc cagacgaagg
     3481 gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg acttcttctg
     3541 atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat acataggtca
     3601 aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca cctaataccg
     3661 gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg ttgaccaaag
     3721 tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt tttaaaagct
     3781 tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta ttgatcgaaa
     3841 caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg atcaataata
     3901 aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca ttctctcctt
     3961 ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca cgttaaaatg
     4021 gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac ctacgaggtc
     4081 gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt gattatatag
     4141 gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc aagcaatccg
     4201 gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat tttcagtgat
     4261 ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat tagcacaacc
     4321 acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa tgcaggtgct
     4381 agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat gaccccaaat
     4441 aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt accaacgaat
     4501 aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag ttgccccaat
     4561 aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt tggcactagt
     4621 ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat tactacggtt
     4681 gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact aaggaattgc
     4741 tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat atctacttcc
     4801 ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt gatgaaatca
     4861 ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac actaagtaaa
     4921 atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca tattcttttg
     4981 atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag aaagcctgca
     5041 atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa aataaagttt
     5101 atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga ccaaaacatt
     5161 agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct tactagtata
     5221 tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt gccatacttt
     5281 ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc atgaaagttg
     5341 tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc aggcatcaag
     5401 tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat gaagcccatt
     5461 gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta cgtcttttgc
     5521 ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa tacagacacc
     5581 aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat caaggctata
     5641 atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa ctaggatata
     5701 cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg aaaaagactt
     5761 tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa atctctcact
     5821 ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg ttataccaaa
     5881 tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca accagaatct
     5941 tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt ttgctgtgca
     6001 tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc ttgatatcca
     6061 agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa gctagattac
     6121 aataatagga aacccccttt tctctgatca ttgctattac attgaaccag tattttttat
     6181 gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat tcagtgacta
     6241 atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc atggtatctc
     6301 gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca aactctcctt
     6361 cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg tgtttgggtg
     6421 ttcattacac cgacatcata attattatat caataaaaat gagctttgcc tagaaaggat
     6481 tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc ttaggaaaaa
     6541 cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg gtaaattctt
     6601 gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc agatgactaa
     6661 aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc tgcttttcaa
     6721 atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt tctggctttg
     6781 tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc attggaaatg
     6841 tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac gctccagaat
     6901 gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt tttgttttta
     6961 caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg atcggacaag
     7021 tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc attgcttggt
     7081 tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca aagattttcc
     7141 tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact actcgcatca
     7201 cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc gcgcctgtcg
     7261 tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc atttccccat
     7321 caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt gagtaataac
     7381 caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc tcttcaattg
     7441 taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact ctggtttgct
     7501 gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc caaggcaggt
     7561 ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt gtttctttat
     7621 ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat gaatcgctgg
     7681 atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag atttcttttt
     7741 ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac taaataatca
     7801 tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg gatcacttct
     7861 tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc tttttggctt
     7921 tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg aacttggtct
     7981 tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc tttttgctgc
     8041 atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt gcgcacataa
     8101 ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc tacgatttta
     8161 tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc tttttccgat
     8221 cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc tccgctactc
     8281 ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa tcgatagcgt
     8341 actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac gcgatagaaa
     8401 gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct cgaacatata
     8461 ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga ataaaccaca
     8521 gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg acaaacggtt
     8581 cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt caaacggttg
     8641 atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat cacgaaagca
     8701 actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa agatcgcgta
     8761 gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca cttagcttat
     8821 caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa attcaaacca
     8881 gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc ttggcattat
     8941 agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat tgaagctggt
     9001 ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt tcaagaacct
     9061 cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga tgcacccatc
     9121 tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat cggtgttaaa
     9181 actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca aaaaaacgca
     9241 atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca aaatgtccag
     9301 gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg aaaagaagcc
     9361 attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt ctttcaactc
     9421 aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga tgtccagcct
     9481 aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag tgtagcaaaa
     9541 caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga tgcaaaaaaa
     9601 aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc agagcaagtt
     9661 ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga tccaccacgt
     9721 acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa aaaacttgtt
     9781 tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag taaaaagttc
     9841 aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg tgaagttgtc
     9901 gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc tgccctgtat
     9961 caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata ctgtacttgt
    10021 gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga catagttttt
    10081 gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata aaatgcattt
    10141 gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg atcctgcgga
    10201 agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc tatttttgtg
    10261 atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg tttggtaatg
    10321 tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat atttaatgtt
    10381 cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa aggctcaaag
    10441 ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt agcttcgata
    10501 ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc tgctgatgtt
    10561 cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc tggaatgcta
    10621 aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat ctttttcatg
    10681 tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta acacaaacat
    10741 tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat gcagattata
    10801 acagaagtag acgagaaaag tacaaataga aaacatgaga atcaaatcat atgatctttt
    10861 tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa ttatcaattt
    10921 ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt aaactaatat
    10981 tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg gtttgtgtta
    11041 taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc ggaaaatatt
    11101 actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg ccgatgtgga
    11161 ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac aacaacaaaa
    11221 aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat tttatatggt
    11281 ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt acacttttag
    11341 ttattttctt gtcagtgctt atgtatcagt tgtggctgca tcatcgcctg gcaatgtggc
    11401 tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat tcaatttatt
    11461 ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa aagtgccacc
    11521 taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact atttttttct
    11581 agtacccact tgttgttcca tactcattat tctggtcata accataacca ttttgttcct
    11641 gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac tgacttgtat
    11701 cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg ttttctgaat
    11761 aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc gaatcgccat
    11821 cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta ggtaatgtat
    11881 cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga agatcatttt
    11941 ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc aactggtctt
    12001 gtttgattgt ttcaaacgcc ggggtgtagt tcgtcgctac atcaagcatg tcatcccaat
    12061 ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa ttgcttacgc
    12121 catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga cgacgttgtc
    12181 gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa cctttttctc
    12241 cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag tcaattttat
    12301 tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc aaattgatcg
    12361 ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg gccatttcga
    12421 caccaccaaa tgcgtaggca tgatttaatt tatctaccgt tccataacct acgatattcg
    12481 tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga ttaactgtta
    12541 ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg gtatctaaac
    12601 ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca gaacgtttgg
    12661 aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct tctcccataa
    12721 atctgattcc gtatgctgat aatccggcaa tcagcaaaac gaatacccct aacaaggtta
    12781 atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct aaaatattgt
    12841 ctcatataac tgttcaatca gcaaattcac tttttaatca taagaaaact ttaaaaaaca
    12901 tttttgtgta acgattatta ttttttaaaa tttctcaaaa ttcttaacta tcataccata
    12961 ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca atcgttttat ctttttttat
    13021 gtattttttc cagaaaaagc gaacaacaag gtattttcca aataatcgtg cagtaaaatg
    13081 atcgcacata cctttcccat ctttttaaca gtctttgcct accgttcaga ttatcattcg
    13141 atttcacgac aatttcttcc tatgactggt ccttttgtta aacccatctt ctattcgttt
    13201 tatttccagc tcactgcatg gctcttaaaa tgactcgaac tagtgattgc attaccatca
    13261 tcttttaatg gatagatatg ctcctcttca aagtaaaacg attttctttt aaagtaatag
    13321 aattgacatg gtaatgagta tgtatactag tggtttcaca aatggtcttt tattcaacat
    13381 ccaaaaactg aaatttcttt aaaatccatt actaacgaag aaaatgttcc ttgcttgata
    13441 attagttagg acatgtcaag tgaacaaatg catttactta gccaaaaaat ctaaaaactt
    13501 cataaataga caaaaagaca cgactctcta a
//
LOCUS       DAZUKC010000065        13401 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000065 DAZUKC010000000
VERSION     DAZUKC010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.066"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(219..779)
                     /locus_tag="ACG80T_001965"
     CDS             complement(219..779)
                     /locus_tag="ACG80T_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4841600.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            921..1535
                     /locus_tag="ACG80T_001966"
     CDS             921..1535
                     /locus_tag="ACG80T_001966"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4841601.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            1724..3604
                     /locus_tag="ACG80T_001967"
     CDS             1724..3604
                     /locus_tag="ACG80T_001967"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS4841602.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            3683..3910
                     /locus_tag="ACG80T_001968"
     CDS             3683..3910
                     /locus_tag="ACG80T_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFS4841603.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            3915..4457
                     /locus_tag="ACG80T_001969"
     CDS             3915..4457
                     /locus_tag="ACG80T_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFS4841604.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            4684..5400
                     /locus_tag="ACG80T_001970"
     CDS             4684..5400
                     /locus_tag="ACG80T_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFS4841605.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(5460..7295)
                     /locus_tag="ACG80T_001971"
     CDS             complement(5460..7295)
                     /locus_tag="ACG80T_001971"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS4841606.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(7491..8489)
                     /locus_tag="ACG80T_001972"
     CDS             complement(7491..8489)
                     /locus_tag="ACG80T_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841607.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            8708..9928
                     /gene="sstT"
                     /locus_tag="ACG80T_001973"
     CDS             8708..9928
                     /gene="sstT"
                     /locus_tag="ACG80T_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFS4841608.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            10075..10710
                     /locus_tag="ACG80T_001974"
     CDS             10075..10710
                     /locus_tag="ACG80T_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFS4841609.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            10901..11641
                     /gene="truA"
                     /locus_tag="ACG80T_001975"
     CDS             10901..11641
                     /gene="truA"
                     /locus_tag="ACG80T_001975"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFS4841610.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(11786..13399)
                     /locus_tag="ACG80T_001976"
     CDS             complement(11786..13399)
                     /locus_tag="ACG80T_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841611.1"
                     /translation="MDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLSRIIGLDNV
                     WSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFITFLTMVVS
                     YFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYI
                     RDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVL
                     TFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFLG
                     LIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSSL
                     FIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPEDAV
                     AIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKNT
                     VLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTKLAIASRYL
                     SIVTLILFLLYRFTFSKQKQPRREVQHSH"
BASE COUNT         4481 a         2122 c         2613 g         4185 t
ORIGIN      
        1 agcctaaaag taatttatta atgatgaggt gaatttgatt ggtacaacaa gatatatcac
       61 ttagagaggg agaagcagca ctacaaacta cttttcaata agtaggaatt atagaaattt
      121 tactcaaaaa atcttcagta aacaatttgg ctaaataaga ttcagttgtt atacgagaag
      181 gttacggttc ttcttaacta agcttagtat gtttttattc aaggcatgat tttctctatg
      241 tgatcattca gtttctgtaa gagttctaat aattcttttt tggagtactt gtaattgctt
      301 tctacccacc gattgagaac ggatagacaa ccttggacgt ggtacgtagt aaagaatctc
      361 atttcttctg tgataatcgt tttcttgtct tcatatagcc atatgttcag atagttttct
      421 tcaataactt gtttaacttt cttctgaaac gaaatatcac catggttgag gataaataag
      481 cgatataaaa aagtatttgc gtaaacatag tcaatcaagc gagtgtatag ttcctcatag
      541 ctatgggctg gatcataaga gagaatttca tttagatccc gaaaaaagtt gctttctaat
      601 tgatcatata gatcatagat atccttataa tgtgtataaa aagtcgaacg atgtacatct
      661 gccgtttctg ttagttcgcg tatagtgatt gcttgaatct ttttatttac taataatgtg
      721 gatagtgttt cagccaaagc tttttctgtt tttttgacgc gtctatctgt ctttttcatt
      781 tacctatctc cttatccgac atatcaaagc aaagctgtcg cttaatcgac aaacttatag
      841 atgattgatt atagatagta gacactgtgt ctattataat gtaagtataa cacaatcatt
      901 gaagttgagg aggcgaaatg atgaaactaa cttttaaacc cttagaaaag aaagattaca
      961 aaaaagctat ctattttgct gttgaaggaa tgaactttga gagatatatg gaaaatgaac
     1021 ttctgctgaa tctttacgga agatattttt tgtatatgga gttgaatcga gcaactcaaa
     1081 ttcttgcttt gtatgcagag gacgagttgg caggtgtttt gctggcggag ataaaaggag
     1141 aaaaaaagaa acatcgttct tttggacagc aaatatatgt taagatattc gattttttac
     1201 aaaatatatt tgctaaagaa agtaaaggag cttacgagac agcaaatgaa caaatgcttg
     1261 cagcatattt aaagaagcat tcgccagatg gagagatcat ttttttagct gcaaatcctg
     1321 atttgaaaac aaaaggcatc ggtaccaagt tgctaacaga atttgaaaaa agagagaaag
     1381 gaaaagaaat attccttttt acagatagtg gctgtacgta tcaattctat gaacgacgtg
     1441 gatttgaaag actaaaggaa aaaattagca agataaaaat cttagataaa gaaatagatc
     1501 tccgatgtat gctgtacagg aaaatcattc aatgaaaatt tcgatagaaa cgacaatagt
     1561 tggcaagatc acttttagtg agtcttcttg ctaactattt tttgtagtgt attctaccaa
     1621 aagaaaaccg cctttttgac taaacttgat agtggtcaat tttttatgat ggataagtat
     1681 ctatacttag tctcgtaaga atgaaaggaa aagagggaga aagatgaaat tacaaaagtg
     1741 gatccaagag catcgaaatc atatcaccgc cttatcagct gtgttgatta tttttggttt
     1801 tatcggaaaa tttactggtt ttgtcttgtt ctataacatt gctctagcca tagcttctat
     1861 tattgcggtc atacctatta tgatccatgc gtatcaggca gtgaaagtca aagttatctc
     1921 aattgattta ctcgttacta ttgctgtgct tggtgccttt atgatcggag aatacaagga
     1981 gtcagctatc gttacattct tgtttttgtt tggtagctat ctggaacaaa aaacattgac
     2041 caaaacgcgg gagtcaatca aggctttgac cgagatggcc ccatctactg ctagtgttgt
     2101 ccaagaagat ggaacgactg aagaagtgga aatcgatgac gtggaagaag gcgatatcgt
     2161 cttagtaaaa actggtggag ctattcctgt ggacggacga gtcattgacg ggaaagggta
     2221 tgcggatgaa gccgcagtga ctggtgaatc acgacaagtc gtcaaaatca gtggagatga
     2281 agtcttctct ggaacgattt tagcagatgg ctacgtgacg attgaagcaa ctaaggtcgg
     2341 agatgatacg acctttgcca aaattatcga attagtagaa gaagcacaag ataccaaatc
     2401 ccatgcggaa aaattcattg atcgattttc tcagtattat actccaaccg ttttacttat
     2461 tgcgttggtt gttggtctga ttagtcgtga ttttcgttta gcaatcactg ttttggttct
     2521 tggttgtcct ggcgctttag tcattggtgc gcctgtttcg aatgtagcag gaatcggtaa
     2581 tggagcgaaa cggggcatct tagtcaaagg cggtgaagca atggatactt tttccaaaat
     2641 cgatactttt gtctttgata aaactggtac attgaccaaa ggaactactg ctgtatccac
     2701 tatcaagaac tatacagagg atgaagggga cagcttagct ttagtggctc gattagaaac
     2761 gttatccgat catcctctgg ggcgcgccgt gatccattat gctcaggaga aagacattga
     2821 ttttcaacaa ttagcagtca aagataatca gacaatcaag ggacaaggaa tcacagctga
     2881 gattgatggt catacagttt gtgcgggcaa caccaaattg attgccgctc acaaactttc
     2941 tctcacacca aagcaagtac aagatttaca tcaattacaa caaacaggca gctcggtcat
     3001 tattgtagca atcgacaaaa aaatcactca aatcataggt gtgtcagatg tgattcgacc
     3061 agaagtcgct gaacaattag ctaagttgaa acaagcagga gcaaaacagt tagtgatgct
     3121 gacaggtgat aatcagatga ctgctgatta tgtagctgat atgttaggca ttgatgaagt
     3181 ccatgctgaa ttattaccag acgaaaaggt tcaatttgtg aaaaaatacc aagaacaagg
     3241 attgcgcgtt gcttttattg gagatggaat caatgattct ccctctttgg ctgcggctga
     3301 tatcgggatt gctatgggat caggaacgga tgttgctatc gaaacgagtg acgttgtctt
     3361 gatgcaatct aattttgaat cgttggttca tgcctaccgc ttggctaaga aaactgtttt
     3421 aaatacccgt gaaaatgtag tgattgccat tggtgtcgtc ttatttttac tcaacggctt
     3481 atttgccggg tttatttaca tggctagtgg gatgttcgtt cacgaagcta gtatcttgat
     3541 cgttatcttt aacgcgatgc gtttattgta ttttggacac gaaagcaaag aagaaaaatc
     3601 gtaaaacttg ataagaatca atttttttat ggaagctgta atctagaata agactatcaa
     3661 gaaaaaggaa ggaagaacta cgatgaaaaa aattattatg caattagata cactagcttg
     3721 tccaagctgt atgcaaaaaa tcgaacatgc ggtttctcaa cagcctgggg taagcaatac
     3781 gaaagttttg tttaatgcga gcaaaatcaa agcaaacttt gatccagatg aagtccaccc
     3841 tgaacaatta gctgcagttg tggaaaaatt aggttatgaa gtccaatcag caaaagtaaa
     3901 agaggcgtaa atcaatgaat atagtggaag aaaaatttgt tcaagaaaca aaacaagcag
     3961 atatcgacca tcataaacca actgctggcg ccatggtggg gcatgtatta gcaaatttta
     4021 aattagaaga aatgaaattg atccaagcac aattgtatgt tttgggtaca tcttcgcaag
     4081 aattaagtac ccttttttca gaatatgctg atcaagaagc gagatggttc aataaaatct
     4141 ctcatgagtt agtgattgaa aatgaaatcg ttccgtctac tttagaagaa gtcatgcgtt
     4201 atggaaaaat ttacgaacat ggtcaaaaca aatatttacc agcctcaaca atgttggaaa
     4261 actttgcaaa agattttgat ttccaaaatg tctttatcac acgggcaatc aaattagctg
     4321 aaaaagaaga aaaatatggc ttgcaacaat tgcttgttga acttttaagt ttcaataaag
     4381 aaatcatcta ccaagtacaa agactccaag gaaaaactgt ccgacaagat ttggatgagg
     4441 aagacgatga tgactaaaag tctatgtttc ttcaaataca aatataaagc gtgaggtaaa
     4501 ttggaaaatc acgaagatta aaataaaaga agcacccgct gatgtattac agacaacatc
     4561 agcgggtgct tctcagtttt tagaagattt gaaagattga tgagaaaata cgaaaaatca
     4621 ataaaatcac attgcttatt tgatagaata aagaagtggg agtaatcgaa aggagttttt
     4681 taattgaaaa aaaggatttt agtaacaatt ggtctagctt ttttgttagc tggttgtacc
     4741 aacgggaatg aatcgaaaaa agaatcaaca aaagaaacag cgaaagcaac agttgcaact
     4801 actacaacaa ctagcacaga gagcaagtca actgcagcaa gtacagaaac agcagatacc
     4861 tctacttcgt cagagtcttc caaaacgact gaaacaactc aaacatctac ttcaagttct
     4921 tctgtagagg cttcatcttc ctcttcagaa agcagcgaac aagttgaaca aacactgtgg
     4981 aatgcagaca aatccgcaca attagaggca tttatgacca aatggggcca aacaatggga
     5041 caacaataca aaagttatac gaatcaaatg agtgtggact tatatggtct taaagtccct
     5101 caagctattt tgaacgggga gtggaagatg gctattggtg gagtgcctgt ttctgcagaa
     5161 tggtcagaaa gtgggacagg tcaagcagac tatcaaatca cagccgtcta ttccgatgca
     5221 gagacagaac catatctgaa aaagcacgtc tatctattcg ggtttcagca aaatcagcca
     5281 aaagtattgg tgacacaaca aaatcagggg aatcccgata attatcttta cttcaacgag
     5341 acagcgaata atgaattgaa aaatggcttt aatcaaatcg tttatggaca agcaaattaa
     5401 tgagataaac aaggggttgt gacatgtctt ttatcacaac cccttgctca gtttatttgt
     5461 tagtctttta ttttttgtgt cgcccatgtt gcactgtact ggttacctgc aacaagatca
     5521 tattgtatca gacgataatc ttctagaatt tctttttgtt tctcagttaa atttttttct
     5581 tggataattt gactatcctc tgtaacaaag acctgttttc cattaaaacg attagacaag
     5641 ctttctgtag gatcaattgt taatgcaggt agatgattag atacttctgt caacaacgca
     5701 tagtaaggag ttactttgat attcgcatgc tcaagagcca tagcaccaaa gttattagga
     5761 gcaacaactt ctttatttaa cgtagctgct tgttcacgac tatacttgtt gttataaatg
     5821 aaataatctg tctggtgctg ttctaatcca tattttttcg aatcattccc actatagatt
     5881 gcgggtaaat ggtcaccata aaaaacaaag gtaatcggtt tatcgatttt gtcgatttct
     5941 tggatgaatt tttttactgc tgtatcggta tagtgaaggc cttgcatata agtttgtaat
     6001 tcttttcttc tattaggaat gacagcagtt ccttcagcag tgtagttcaa ttcactatag
     6061 taatcatcat aaggcatatg gttttgcata gtggataatt ggatgaattg tgacgtttcc
     6121 gtattgcttt gtaaagcttt caaggtttct ttgtatgctg aatcatccga tatgcgtggg
     6181 ctttgttcta ttttatccgt ataagttaat ttatccggac tgtctaaata atagaacctg
     6241 tcaaaaccaa atttatcaaa cacttgtttt ctgcgataaa gagcagctgt aaaaggatgg
     6301 atggctattt tttcatcgaa tagatcggta atatttggac ttattttctg acgctccact
     6361 agctgggtat aaggtgtaac taacgaagca gacaggttac tgatactcaa gcctgtcagt
     6421 ccttcccatt ccatgttagc tgttccgcca ccataaccga cggataacat caatcctcct
     6481 gttccttctg ttataatgct tttgatgtaa ggaatagggt ctgtaggtac tgtcagatta
     6541 gggacacgtg caggatcact gaagctttca cttaaattga gaacgatcgt ctgattgttt
     6601 aaccagtcac ttcttgtttg gttgatttca tctgcctttg tgttgtactt tttcattatt
     6661 ccattaattt tttctttcga atagccagca ggttcttcca tgattttaat gtcgatattg
     6721 tttaggaatt gaatgactgg tccgttatct tttacggcgc cttgctggtt aaagaaagtc
     6781 ttgtttactt taaataaatt aaacagcaag aaagaaggcg aatttttgtg attaatgaaa
     6841 aagacacccg aaaaacatcc taataataca gcgatactga taaatcgacg tttccaatga
     6901 atcttcaagg aatactgttt ttgtagtcta cgttgtacaa taatactagt aatcaataat
     6961 aggagaagga tcaatccgcc cacgattaat aagactgggc taaccatcga taatagttct
     7021 gacaatccgg tcagcatttt taaatctgaa ggaaagacag gctcttcccg catttccatt
     7081 ttgattactg tcgaaatagt gagcagtaag ttaatgatca aggtagcagt caaggcgtac
     7141 caaaaacgat tggaaatcaa caatagtaag aagaaaaatc ccataataat gatgattgtc
     7201 aggatcatgg gtgcttgtcg ctgagtaaat atttttaagg caacctggat agtttctttc
     7261 caaccttctt ttggttctaa aacgaaaatc tgcacgaagg taaagcagta aaaaaatgct
     7321 gcaactaaaa aataactttt atgaacaaaa agccaatgaa ttcttttttt ccaacgctga
     7381 gttaattgtt caatcgaatc aaaagcgaag tattcgatga aacgatgaat gaaacggatt
     7441 ttttgtatat aaaataaaaa gataactagg agggacactg ctaataaata tcaaataaaa
     7501 aactgcccga tatttatcag ccatgccttc cctgagtttg gaaattcacg tttcataaat
     7561 gtagatacgc gataagaaac taaagggaaa ttacatataa cttgagtgac aatcaaaaat
     7621 acagcaagaa aatttcctct tccttttata cctaatttat cagatagcat ttctaaaaat
     7681 aaaaacgaag agaaagtata atacattgcg aagagagaat atggtttaga aaacctcagc
     7741 gctgtatctg tatttcctct gatgatgagt gaaatttgtg tcatcaaaat aatcaaaccg
     7801 aataaaacaa tcaagctgat aaatacttga ggaagcttat agcggacctt ttctttccag
     7861 ttccattctt tcaaaccata acccattaaa aataaataaa aaatccataa aatactctgt
     7921 ccattctgta atgcaaacgg gtctttatta aatagagtag agcagccaac taatccaatt
     7981 gttaaaaata atagtagata tttaattgtt gttttcgata atttatttaa aaatccaact
     8041 gtataaggaa tactacaaaa aagcaaagta caagaaacag cataagtaaa gtaattctga
     8101 ctgataggtg ttaggataag ccaataatca cgaatattca agctgccagc ttgtacgtaa
     8161 aaaaagatat tgcaaactag tgtggctatt aggaatataa aggcaaaacg acttaaataa
     8221 tgtaaaactt ttaaacgggc gattttttga tattggtaac ccatgtaaag tgggatcaaa
     8281 tttgcaccaa taacaacgga agtttgtaaa aatgtataga tgtagaatcg accagggttt
     8341 gttgcatatg taggaatagt gactgctgaa atcaatatac gctgtaaaac catcattata
     8401 caagcgacaa agattgtcag gaagccataa ctctccttct ttattttttt caagaataaa
     8461 cacctcacaa aaaaattgga caaaaacata agaaaacgtt tcctgataag ttgcccatta
     8521 ataacatcct aacttagaat atctcaaaaa acaacgtgta gacagaaaaa gtacaaaatt
     8581 agcaagaaat ggttgtttgt attgttagaa tttttagaaa attatacctt tagtttcttt
     8641 gcataaaatg atacaatgat tgttaaaatt gtgaaagggt ggtcatttgt tagagaagtt
     8701 tttggatatg tcgttagttc aacgtattgt tgttggtatt ttagtcggaa tattgtttgg
     8761 tatctttttc cccacatgga catttatttc aatattggga acgttgtttg tcagttgctt
     8821 gaaggcaatc gcacctattc tggttttttt cttgacgatg gcgtcaattg ccaagcataa
     8881 aattgggaat aagacattcg tgaagcctat tttgatctta tatttagtgg gaacattgct
     8941 ttctgcactt gtagcagtta ttgtaagttc aatttttact gttcctatta ctttacaaga
     9001 aacagtatct gaaaaagcac cgcaaagttt ggaaagtgtt ttatctacta tgttgacgaa
     9061 tgtcacacaa aatcctgttc aatctcttat tgatgcgaat taccttggcg tcttattctg
     9121 ggctgtattg ttgggactag cttttcgagc acgttcagaa tcgacaaaag agttggtgga
     9181 tcagatatct atagcgttgt cacaagtcgt acaagtgatt attgcatttg ctccgatagg
     9241 gattttagga ttggtttatc aatcaattgc tacgactgga atcgctggat tggcagagta
     9301 tctgcagctt ttacttgttt tgatcggtac gatgctgttt gtcgcattag ttgtgtaccc
     9361 attcctcacg tttttgttta ttaaagaaaa tccctatcca ttgattttct tttgtttgaa
     9421 agaaagcgca cttcccgctt tctttacccg aagttctgcc gcaaatatcc cgataaacat
     9481 gatgttggct gaaagattga aattgacaaa agagtcttat tccatatcga tcccgttagg
     9541 tgctacgatc aatatgggcg gtgccgcgat taccatttcc ttgatgacgt tgactgctgt
     9601 acattcttta gggattgaag caccttttgt tttgaaagtc cttttatgta ttttgtctgc
     9661 tttagctgct tgtggagctt caggtgttgc gggcggttca ctgttattga ttccattagc
     9721 atgtagtttg tttggtatct ctaatgatgt cgcgatgcaa atcgtcggta ttggatttat
     9781 tatcggtgtt atccaggatt caatggaaac tgccttaaat tcgtcaagtg atctgttgtt
     9841 tactgcaata ggagagctag gcgcgcgtaa aagaagtggg gaaaaaatca aattgaaaga
     9901 ttgtttagat ggattaagtg aacgttgaac gttagatatt tagtattttt aaaatgattc
     9961 gagtttttaa tgagttatat agatgttcgt gtaaatagag gtttctgtta aatcaagata
    10021 aagtcgttta taataacaat taaaagcata ggtaattgtt attggaggcg tatcatggat
    10081 tttttacgta aatttggttt tttcttttta ttattaggcg tgctgagtga ttttttgact
    10141 ccgtacgtat taggattgta ttatcctgaa ttagatcaaa tgaagagcgt gatcagtgta
    10201 tttggagagg tttccagtcc tgtaagaaaa gcgtttctta tttggtctgt ggtttcaggg
    10261 atctttttca tcctgtcatt gcctgcattg tatgctgcat ttgttagtac atctcgtgtg
    10321 ttagctaccc tagttacagc agccatcgcg ttctatggta taggtgattg tatctttaca
    10381 ggtttgttca gtatcaatac ccatgaatct tcgtggaatg tctcgacatg ggtccataat
    10441 atcggatcag gtttaggtta cagtggtttt ttattgtttc cagttcttct tttcttgatc
    10501 tatcgaaaaa atgggcaaat ggacgtaagc cacttatacc ttggactgtc tgtgatcagt
    10561 ttgctttttg cggcgattta tggactggca agaattcctt ctataaattc ttggccatta
    10621 ctgaataaaa taggtttttg ccaaagaatc agttttttct ttaattatgt gcccattgcc
    10681 tggttgagta tcttgcatct caaacagtaa aacttgagcc gtcaaattga acagtcagcg
    10741 gatacttcat ccggtggctg ttttttgttt ttctgaaata tatatttcca cagttttctt
    10801 gtatagcttc tgtcttagag aagcaaggag ctatattttg tgctacaatc ttaaaaaggt
    10861 agagagaaga ccaaatggaa gacagaggag ccactaaaat atgagaaaca tcaagttgac
    10921 aatcgaatat gacggaaaaa gatattcagg atggcaaaga cttggagatg acgataaaac
    10981 cattcaagga aaaatcgaaa aagtattgca tcagatgaca aatgaagaga tagaaattat
    11041 tggatcagga agaacagatg ctggcaccca tgcgcgagga caagtagcaa attttaaaac
    11101 aaacacagaa attggactgg cagaaatgat cgactttatg aatcgttacc ttccgcggga
    11161 tattgtcatc aaaagaatcg aagaaatgcc tgagcgcttc cattcaagat ataatgcggt
    11221 gggcaaaaaa tacagctatt atgtttggaa caacgtgatt ccttcagcat ttgagcgaaa
    11281 ccacagcttt tatttccctc aagagttgga tatggacaag ctgaatgcag cttgcgaaaa
    11341 attagtgggt acacatgatt tcattggttt ttctgcattg aaaaaatcaa aaaaatccac
    11401 agtgcgtacg attgatgaat tatctatcga acgaaaggga gaaatgcttc attttacttt
    11461 tgtcggagac ggattcttat ataaaatggt gcggattatc atggggacat tactagaaat
    11521 cggcacaggt gccatgccga tagaaacaat cgatgaaata tttgaaagca aagtgcggca
    11581 acatgcagga gaaaccgtcc ctgcacaagg tctgtttttg gatgaagttt attatcgata
    11641 aaaatacttt gataatggat gaaacgcatg tttcacagta taaatatcga tgttaaataa
    11701 tgtatgaagc aaattaaaga aatataaaaa aagacttttt agcctgttta tgtgtacagc
    11761 taaaaagtct ttcttaaata aaaatctagt gagaatgttg tacttctctt ctcggttgtt
    11821 tttgtttaga aaaagtaaat cgataaagta aaaataaaat caaagtaacg atagataaat
    11881 atcttgaagc aattgctagt tttgtgtact gataagtaat aacgacttta tttatacctg
    11941 gaggaatagt taattcagtt gtgccaaact tagataattt tgtttgtact agtttgccat
    12001 taatagaagc cacttgccct ttataacgat aaataggtgt cgtcaagaca gtatttttat
    12061 tattcgaatt atctaattca attgtaagtt tgttactcat aaaatgagtt gttggattta
    12121 tttcctggtt acctagtaaa tattgatcat tattaatcat atcaggatga tagattgact
    12181 ctttgtttgc ataatcagta tgattgtaat tttcagctat agcaacagcg tcttcaggtg
    12241 ctataagtgt taatgaatct ttgttagcat ggatagaatt gcttactcct gagagatgca
    12301 atattagtgc aagaaacgta aagaccatta ttttccatga attattttta gtagaaaaaa
    12361 tataatagat cactatactc atcgtatagg ctataaataa agaagaaaaa ccatttaacc
    12421 gccagacaaa ttgtagactc ttaactggtg tatggttaaa aagttgccaa ggaaataaat
    12481 tggttgaaca aaaaagaaca aatacaccaa gaaagaaaat aaataaatca tcagcagtta
    12541 atttttttat aaaaataaaa gcaccaatca atccgagaaa taagaataat ccaatagtat
    12601 agttgttata gttattattt aaaatatgtg tcagcatatc actaggagcc ataccactta
    12661 attcttttcc cagtgggact ttcaattctt gtgctctaga ttgttgaata aaaggaatta
    12721 agaatcccgc acttaaaaaa aaggtgagga cggtagcttt taaaagactt aagagtctag
    12781 cgattttttg gtcccaaaaa taaaaagaaa gaaacaacgt tcctccaata aatacagaaa
    12841 ccatagcgac agaaagtaaa tgagtataca ctactagcgt catgccaata gacaaccagt
    12901 accatttttg gtaatctcta atataaattt cgtaacaccc cattagtatc aaaggcaaaa
    12961 atgttaaagc gacagcttct cccaatgaag cacgatggaa tatgtcaatg gcacgatagg
    13021 cggaaaatgt ataaataatc gaaaataaca gcgaaatata acgattgttt tttatttgtt
    13081 tcatagaaaa ataagaaacg accattgtta aaaaagtgat aaaaaaatag tagatattat
    13141 aacttaaaac aagatttccc atcattttat aaaataaaaa agctggataa agagtaagcc
    13201 atggataaaa aatattcatc attgttccat gatgatcgaa gttattgaag tttactggag
    13261 aactccatac attatctagt cctatgattc gagataaatg aaagtaagta tcctgattga
    13321 cactatgata gattccctta gacatgaata gagggatgac gtaagcaaaa cttagaaata
    13381 acataaaaat gatatctatt a
//
LOCUS       DAZUKC010000066        13323 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000066 DAZUKC010000000
VERSION     DAZUKC010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.067"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..429
                     /locus_tag="ACG80T_001977"
     CDS             <1..429
                     /locus_tag="ACG80T_001977"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841612.1"
                     /translation="MIIQKFDWKMKLGISLVILGFIIFLGIKAQKNQSTFETMSSQII
                     EECSKIYYCENKTEESNGFYYIDQPHDVVIKMESHEVNEWIQRSTNVSKKLERYSQTF
                     LSKVIFSNCKNEETSQRVASSLENLKFSVSKKEKFVDLRK"
     gene            664..1251
                     /locus_tag="ACG80T_001978"
     CDS             664..1251
                     /locus_tag="ACG80T_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841613.1"
                     /translation="MENNNDIRYFSIGERVTTVSELSKDYRDKEGTIISLKKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNDGSHSIEKAILVFPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITNKEAKVEDVLTVRELQSLIEYRNKQITNPEYLKKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDNFKKIYLKEIEKD"
     gene            1251..2150
                     /locus_tag="ACG80T_001979"
     CDS             1251..2150
                     /locus_tag="ACG80T_001979"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0000400 - four-way junction DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFS4841614.1"
                     /translation="MWSVTLPFQPVSAPRPSARRNVADATNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQGLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            2150..3064
                     /locus_tag="ACG80T_001980"
     CDS             2150..3064
                     /locus_tag="ACG80T_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841615.1"
                     /translation="MNNNEKILHVLDSFETIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKKAMELNEDRRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPANSGIGIVLKAIDEEEIEDFIPAEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCEGYEVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     DARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNASNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKEAE"
     gene            3116..3331
                     /locus_tag="ACG80T_001981"
     CDS             3116..3331
                     /locus_tag="ACG80T_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841616.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLQQFWLNNFSYKDFIKEKANRLY"
     gene            3521..3775
                     /locus_tag="ACG80T_001982"
     CDS             3521..3775
                     /locus_tag="ACG80T_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841617.1"
                     /translation="MKKEELIDMFQIVERANNMGIMFFDRISLKMDLSVAHQEFNLRL
                     KALLISDDVNFAHDVVGIQNHIDRENKRMGDGFLPRYSSL"
     gene            3987..5003
                     /locus_tag="ACG80T_001983"
     CDS             3987..5003
                     /locus_tag="ACG80T_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFS4841618.1"
                     /translation="MGLVLLSPSVSAESSISMYRMYNPNSGEHLYTRSVGERDNLKRY
                     GWHYEGVAWQAPTSGSPVYRLYNRYNGEHFYTVDAGERDKISHTGWTYEGVAFYSYNG
                     ANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNSAPNPTPAPAPS
                     NEKLLNVPVIWQGNTMLCEGASLLEGLHYKGVATNQNLTSFVNYMPRANDNNPYHGYS
                     GEWRYNVSGTYQGIMPDPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGNPVVTWITYN
                     YASPQFKQMPWGRAVWNGHVVLVDGFKNGAYHVVDPVFGAKWINASTYERAFNVTGMA
                     VAVR"
     gene            5178..5408
                     /locus_tag="ACG80T_001984"
     CDS             5178..5408
                     /locus_tag="ACG80T_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841619.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEEEYKENIPENLQGS
                     ERYEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            5444..5668
                     /locus_tag="ACG80T_001985"
     CDS             5444..5668
                     /locus_tag="ACG80T_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841620.1"
                     /translation="MIDISLIEKLLFSNKYTAKELATRWQISESAIKAYRQNKGSVGW
                     RDWKGISIGKAEDIMKREGFQADNDSKKVE"
     gene            5885..6760
                     /locus_tag="ACG80T_001986"
     CDS             5885..6760
                     /locus_tag="ACG80T_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841621.1"
                     /translation="MTTNKRWSELVQNTVKKVMPDGYHFNYIQCSQTTESIYFSILNE
                     NRLYYFRVSSHHRKKREDNVTTFLSGKYNGFKELSEAIGEYLKSNRFVRISDKHILIL
                     LLYLNNSFKLFWRDDTFYYIENGMDIYSGINGILKHYIKALLRYGVLYQEDLSNQIKI
                     SNSGKSLLAKYKKLLSPIYKGIDMEAVNSETLFNLVVDEKFANEFFKSKKIERLNNLF
                     PENAIKAYVDNNKSRLYNWFIPKRLRGKIKIGDQVKVINLNHTKIVTVTELYIAEKEE
                     ITKMKPVIKILDNKE"
     gene            6770..7564
                     /locus_tag="ACG80T_001987"
     CDS             6770..7564
                     /locus_tag="ACG80T_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841622.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSRRLRITVEYGTNESATELKQRYLDTRREASKKYDDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNIL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            7752..8711
                     /locus_tag="ACG80T_001988"
     CDS             7752..8711
                     /locus_tag="ACG80T_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841623.1"
                     /translation="MGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFN
                     EAYSRIMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLKTYMLFLVNFEGTAK
                     EGTYANPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKK
                     GLLKEYFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETNTFDKYSMYNRAYS
                     SMVAGVFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTI
                     SKEEVEKALEFKNKGFENKGSFGNSKDEVKGVVIDDDFEFDFD"
     gene            8744..9064
                     /locus_tag="ACG80T_001989"
     CDS             8744..9064
                     /locus_tag="ACG80T_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841624.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            9319..10212
                     /locus_tag="ACG80T_001990"
     CDS             9319..10212
                     /locus_tag="ACG80T_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841625.1"
                     /translation="MRTHYIYLEDKIIDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EDEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHEELSEGTEKYTIADMVNEVETLLASFFENGHVNDEWRKEEPKRVNAYI
                     ARLKRFIDKYKENSDKFSKDK"
     gene            10232..11866
                     /locus_tag="ACG80T_001991"
     CDS             10232..11866
                     /locus_tag="ACG80T_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841626.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNNGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMSLTIKRIYDLLYVYKH"
     gene            11895..13064
                     /locus_tag="ACG80T_001992"
     CDS             11895..13064
                     /locus_tag="ACG80T_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841627.1"
                     /translation="MTKKIEVYVDGSQEERDSFFDFFWKNSREYVKALNLVYKQLMFE
                     ELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKLDKAKAELSKLRNEKNKEAR
                     EILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASKDFGNDFIGIVTGQRTPRIY
                     KNRSKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLGSKPRKARDVASVLQHIMDG
                     DYKLNQSNLIFDGRKLFFNLTISFEKRYNELEFKEGNELIVNFADLCSGECLVNNKSV
                     MSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRKLDQDRWEAIKKRESNFVKT
                     YNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLKEQLVNKGKRYGIEVRS"
BASE COUNT         5154 a         1629 c         2744 g         3796 t
ORIGIN      
        1 atgattatcc aaaagtttga ctggaaaatg aaattaggaa ttagtcttgt aatattggga
       61 tttattattt ttttggggat aaaagcacaa aaaaatcaat ctacttttga aactatgtct
      121 tctcaaatta ttgaggaatg ttccaaaatt tattattgtg agaacaaaac tgaagaatct
      181 aatggctttt attatatcga tcaaccacat gatgttgtta taaaaatgga gtcacatgaa
      241 gtaaacgaat ggattcaaag aagtactaat gtatcaaaaa agttagaaag atattctcaa
      301 acgtttttaa gtaaagtcat tttttcaaat tgcaaaaatg aagaaacgtc tcaaagagta
      361 gctagttctt tagaaaattt aaaattttct gtttcaaaaa aagagaaatt tgtagattta
      421 cgaaaataaa ctatcgaaag tagtatgatt atgatcttaa aatatcatct taagagaaga
      481 attacgattg tttataggat ttacaaaatg aataagtttg aagagtgatt tggtcgcaga
      541 ctagatcact ctttttctgt aaaaaaactt tacacttatt tttgagtgtg ttaagatttt
      601 ttatatagag tgacggtttc ttgtcagagt gaatgataac catcaatatt ggaggaataa
      661 taagtggaaa acaataatga tattagatat tttagcattg gggaaagagt tacaacagta
      721 agtgaattat caaaggatta tagagataaa gaaggaacga ttatttctct taaaaagaat
      781 cgaagaggtg tttggcaata tgttgttgat ttaggggatg gcgttacagt tgagtgtaaa
      841 gatagtcaac tgatcagaaa tgatggcagt cattccattg aaaaagcaat tcttgtattc
      901 ccaaaagtat caagcaagat tatgaatgac tggttgcttg gaaaatttgg tgagtacaat
      961 aaaaaagaaa tcgcagaagt gatcggcaaa tcttatccaa cagtattagg gtatctaaaa
     1021 aagataacga acaaagaagc gaaagttgag gatgttttaa cagtaagaga acttcagtca
     1081 ttaatcgaat atcggaataa acaaataaca aatcctgagt accttaaaaa agaagatggt
     1141 ctattgaaac aattggaaca ggtaaataaa atccggaaaa tgcaaaaggt agctccttta
     1201 agcctagata actttaaaaa aatctatttg aaagagatcg aaaaagacta atgtggagtg
     1261 tcactttacc gtttcaacct gtatctgcac caagaccaag tgcaagaagg aatgttgctg
     1321 atgcaactaa tgacgtagca gagaaaagga tttcaaccta ttatgataaa aagtatctcg
     1381 attatttgaa aaaaataaaa agctttatcg aagatcaagg tttgttagat gatgaatttt
     1441 actcgatcgt aaatgatccg atgggtgcaa ttatggaagt ggacttttac tatcagatac
     1501 caaaaagcta taaaaaagtc tataacatta tgaagacgac agcgccagat ttagataatt
     1561 tagtaaaaag tgctatggat ggtattttca acatctcagc tatacgagat agccatatca
     1621 cagggttaat tgcttttaaa tacaatgtgg caaacgaagg aaaaacagtt gtgaggatca
     1681 agcgatattc agaatttgag tgggatactt ctgaaggatt gaacggagat atttgggaag
     1741 ccacatttga tttcaaacca gtagcaacgc caagaccaaa gtctaaattt agaggaaatg
     1801 ggattatcac ttactatcca aaagagtata acgactattt ggaaaatatc aagaatacgc
     1861 ttaaagagaa agatttggta aatgatcaac tgaaaaaggt aatgaatgct cctatggggg
     1921 ttatagctca gatagattat ttttaccaag tgagaaaaga taagaaaaag cttgattcac
     1981 tgatgcgtac ttcacagccg gatattgata acttagtcaa aggaacattg gacggaattt
     2041 ttaacaagga aattggcatc aaagatagca gggtggtagg tttaatagct tttaaataca
     2101 atacttttga aaagtcgaaa acaaacgtgc gactacagga gatgggctga tgaataataa
     2161 cgaaaaaatt cttcatgtac ttgatagctt tgaaacaatt caagaggaac taaagaaata
     2221 tcgagatgtt ttggaacaaa gatatgattt tgtcaatcag caaaagagta atcacatgga
     2281 tttcattctc aatatgaatg acttgaagaa gaaattagtt gaacgaaaag aacaagaaaa
     2341 gttgatcaaa gcttactttg aactcggtga aaaagaagtt aaaaaggcaa tggaactgaa
     2401 tgaagatcgc agggttttag atcagctgtt agaacaatta ttggtgatgt ttcaaaaagg
     2461 acgaatagat gaggatttaa ttgaagaagg gttgagaaag tatcctgcaa actcaggtat
     2521 cggaatagtg ctgaaagcga ttgatgaaga agaaattgaa gatttcatac cggcagagga
     2581 ttttgaatcg gcaatggaat atataaaata ttacagccaa gggatcactg cttttagaga
     2641 gtttgatcct gaagatgtta ttcatgactt gaacaaccta aaagaatggt gtgaaggtta
     2701 tgaagttgat gattccggtt tggactactt gatcagtatt atggaaatcg aagaagagat
     2761 gccggataag cctgatccaa cagatatttt agaattaatc catgatgcta gaaatccaat
     2821 tgcatacatt tctagaggat atactgtatt ggaatactat aagccttata tatcagcgat
     2881 gaaccattta agacgtgtat taagggaaaa acgtgaatac cgctcagtgt taaatgcatc
     2941 gaatagatta gaaaaagctg tttcagagct agatgcttat tatcgtgaac attacctgca
     3001 agcaggtggt atgccaagaa atacgaaagc aaatatcagt aggtatataa aagaagctga
     3061 gtaggcggag ttagcccata aaatggcaaa aggtaacctg aaaggggcgt tttgaatgaa
     3121 aagggatgtt tttgaattgt acgtattaga acgtctagaa gacggcatga tggcacaaga
     3181 gataattgat gaaatcgatg atatgatcag cagatttggt caattggaat tgaatgatgg
     3241 aaacaaaaat tcggtagtgc tacaacaatt ttggttaaat aatttttcgt ataaggattt
     3301 tatcaaagaa aaggcaaata gattgtactg actaaattca cgaggatcag tgatgagagc
     3361 ttattgctaa gagcagttag gctctttttt gtttgtgtta aattatttta ttttacaata
     3421 cacatttata cacaaaaaat gttgacggat agtacacaaa tgtggtagga tatatttgta
     3481 agcaagttag attaacaagt taaaggaagg aagagcagtt atgaaaaaag aagaactaat
     3541 agatatgttt caaattgttg aacgagcaaa taatatggga attatgtttt ttgatcgtat
     3601 ttctttaaag atggatttaa gcgttgccca ccaagaattt aatctacgat taaaagcttt
     3661 attgatttct gatgatgtta atttcgctca tgatgttgta ggtatacaga accatataga
     3721 tagagaaaac aaacgtatgg gtgatggatt cttaccaaga tattcaagtt tatgatttgg
     3781 cgaaagattc aagagtatgt gtcattattg ataggtactc ttgaattaga aaggaggaat
     3841 gaatttaaga caacaacgat tatgatctta aaacaatcgt ttatgtaatt ggcaatctta
     3901 cgaataattt tcatgcaata atgacaataa aaaatgaaaa ggggtaaaat caaatgaaaa
     3961 aatttttagt aggaattcta ttcacaatgg ggctagtgct attatcacct tcagtttcag
     4021 cagaaagctc tatttccatg tatcgtatgt ataatcccaa tagcggagag catctataca
     4081 ctagaagcgt tggagaaaga gacaatctga aacgttatgg atggcattac gaaggtgtcg
     4141 cgtggcaagc tccaacaagt ggttccccag tttatcgctt gtacaacaga tataacggtg
     4201 aacattttta caccgttgat gccggagaaa gagataagat ttcgcacaca ggatggacgt
     4261 acgaaggtgt tgcgttctat tcttacaacg gagcaaatag agttcctgtg acacgcttgt
     4321 acaacaaata cgtgaattgg caccattaca cgttagatca aaatgaaaag aacgtaatta
     4381 gtaaacaagg ttggaagatt gaaggtacgg gttggtatgc aatgccaaac agcgcaccaa
     4441 atcctacacc tgcacctgct ccaagtaatg agaaattgct aaatgttcct gtcatttggc
     4501 aaggtaatac gatgttgtgt gaaggtgctt ccctgttaga gggattgcac tacaaaggag
     4561 tagcgacaaa tcaaaactta accagttttg taaactatat gccaagagct aatgacaata
     4621 atccttatca tggctattct ggcgaatggc gctacaatgt gagtgggacg taccaaggca
     4681 ttatgcctga tccagtagtt aaatgggcaa aaaatgttgg tggtaatgcc gttaatatca
     4741 ctggttcagg aacgaatggg atcaagaatg aaattcgcaa tggtaatccg gttgtaactt
     4801 ggattactta taactatgct tcacctcaat tcaaacaaat gccttgggga cgtgcagtct
     4861 ggaatggaca tgtggttctc gtggatggct tcaaaaatgg tgcataccat gttgttgacc
     4921 ctgtatttgg cgcaaaatgg atcaatgcaa gcacgtatga gagagcattt aatgtgactg
     4981 gaatggcggt tgctgtacgt taattgatag aattaataaa taaatgaaat aagagcttaa
     5041 ttactgaaat ggtaaatggg ctcttatttt ttagaaaaaa ctttttttgt aatacgaatc
     5101 tataacaaaa aacgttgaat atagtacaca aaagtggtat attaaaaccg tagagattat
     5161 gcgaagaggt gattttgatg aaccaaagaa gaagaaaaag gctagaagaa ataagcgagc
     5221 gactatccga gttaaaggat gagttagaag taattcaaga agaagaggaa gagtacaaag
     5281 aaaatatacc tgaaaatcta cagggatcag aaagatatga aaaagcagaa gaatccgttg
     5341 atcaacttga agaagctgtt gatggtttat ccgacatatt agaattgatt gaagatatag
     5401 ctagctagag atgattaatg aagatttcag gagtgagcaa aaaatgattg atatttcact
     5461 gatagagaag ctactttttt caaataagta cacagccaaa gagctggcta caagatggca
     5521 aatatccgaa tcagctatta aagcctatcg tcaaaataag ggaagcgtag gatggcgtga
     5581 ttggaaagga atcagcattg gaaaagcaga agacataatg aaacgtgaag gatttcaagc
     5641 tgacaatgat tctaaaaaag ttgaatagtt taatagtcgc caagaaaagt accagggcga
     5701 ggggaaaggt agattacaaa ggaaaacact tttaaataaa cttcgcaaaa gcatcaggtt
     5761 atgagtggct agggctatac gaatgtgatg aaaacaaagg gctttaatgt caaagaaaac
     5821 gcaatagggg gggaataaaa tttttaaaaa agatatttat gtttggttta atgggaaaaa
     5881 atatatgact actaacaaaa ggtggtcaga gctagtacaa aatacagtta aaaaagtcat
     5941 gccagatgga tatcatttca actatataca atgctcacaa acaactgaaa gtatttattt
     6001 ttcaatatta aatgagaata gactctatta tttcagggta agttcacacc ataggaaaaa
     6061 gagagaagat aatgttacga catttttatc gggaaaatac aatggcttca aagagttaag
     6121 tgaagcaatt ggtgagtatc taaaaagtaa tagatttgtc agaatttctg acaaacatat
     6181 attgatcttg ttactttatt tgaataatag cttcaaatta ttttggagag acgacacttt
     6241 ctactatatt gagaacggca tggatattta ttctggtatc aacggcatat taaaacatta
     6301 tattaaggcg ttacttcgat atggggttct ttatcaagaa gatttaagta accaaattaa
     6361 gattagtaat tctggaaagt cattactggc aaaatataaa aaattgcttt ctcctatcta
     6421 caaaggaata gatatggaag ctgtaaattc tgaaacttta tttaatttag ttgttgatga
     6481 aaagtttgcg aatgaatttt ttaaaagtaa aaaaattgaa agactaaata acctgtttcc
     6541 tgaaaatgca ataaaagctt atgtagataa taataaatca cgattataca actggtttat
     6601 tcccaaacgg ttgcgaggaa aaatcaaaat tggcgatcaa gtgaaagtaa ttaatctaaa
     6661 tcatactaag atcgttaccg taacggagtt gtatatagct gaaaaagaag agattacaaa
     6721 aatgaaacca gttattaaaa tactagataa caaagagtga gtggaatata tggtagcaaa
     6781 aaagaatact ctaaaagaag cttctgaaaa atatcaagat atgaaaagac gaggattaat
     6841 cccaaaggac aaaaaaggta tatggagaga atttgttaag gaagtcagta gtgatcgaag
     6901 tggcggtgac tttgttggac gatatacagc aaaaggttat agcaggcgct taaggatcac
     6961 tgttgaatat ggaacgaatg aatcagctac agagctgaag caaaggtact tagatacacg
     7021 aagagaagct tcaaaaaaat atgatgattt caagagcaga ggtctaattc ctaagaagaa
     7081 aaaggggatt tggcgtgagt ttttcaaaga agaagatagc aatcggaata agggagactt
     7141 cattggacga tacacagcaa aaggctacac taagaattta gggatcaagg ttgaatacgg
     7201 aactggtgaa agttcatggg aattgaggaa tcgctattta aatatattgt tagagaaata
     7261 taaagcagaa ttattggagt tgttcgatgt taatgatttt atcgagatgg ctgaaaagta
     7321 tggtcatgaa gaaggtttta tgatcaatga cattatggtt attgataatt ttacggattt
     7381 attagcactt gcgaaaaaag caagggattt aagagaaacg cagatgtatc tattcagaaa
     7441 taaaaacgga agaaaccgga gaactgaatc gagtgagatt gaagattact acgatgcctt
     7501 tacacacgat ctgggagcct ttgtagacga gataagtggt gtaatgaaca aatatagggt
     7561 ctagaataaa aaagccggag atgagcagat aaatgggaaa caatagcata tacacacctg
     7621 aaatgcagaa taaaattaaa agacttgtcg gattagggtg gatgacaaga ggaaaattag
     7681 attcttttag tacgatcaaa tatcttttga tggacattta ccgtcagaac aagcaaacag
     7741 gaatacattt tttgggctat gataagccgg aagatgcacc tttctttgtt ccaagttcag
     7801 cctatagagc cagtacctcc ggtagcatgt acaacttgga tttgttcgag gaaggagcaa
     7861 agatgatcga atatgatttc aacgaagcct attcgaggat catgagaaat tatcctttgc
     7921 cgtcaaacac ttatttgaaa gcaaaatata atacagatca agctattttg agagcgataa
     7981 atagaccaaa agtaagaccg tatgaaaatt taaaaacgta tatgttattt ttggttaatt
     8041 ttgaaggaac agccaaagaa ggaacatatg ctaatccaaa atcacagtcg atgtttgcgg
     8101 attatggtga aaatcccaaa acagctaatg agcctatttg gttatcggag attgagctaa
     8161 cacttatcct tgattattat gatattaaag agatggaagt actagatagc cacacatttt
     8221 tgacgaaaaa aggattactc aaagaatatt ttgaaagaat cgagccatta aaaactgatc
     8281 ctgatacgtt gaagctttat aaaaatctta gaacgaaagt atttggtgct atcggacaaa
     8341 aatcattaag agaaggagaa acaaatacct tcgataaata cagtatgtat aatcgagcgt
     8401 attcctccat ggtagcaggc gtatttagag atcgtatggt tagatatgaa cagaaatacg
     8461 ttcatagcgc atatgggcta ttaaaaattc gtactgatgg attgtatttc agatccgaag
     8521 taccagagtt tgaagagctg tataaacttg gagtagttaa gaaaaaagtt catacaatat
     8581 ccaaagaaga agtcgaaaaa gctttagaat ttaaaaacaa aggatttgaa aataagggct
     8641 cctttgggaa ctctaaagat gaagttaagg gtgttgtgat agacgatgat ttcgagtttg
     8701 attttgacta aatactcttt ggaaaagatg gtaggaggta aatatgaaga agaaaattgt
     8761 ggaaggaaat gtaggtgtta ttattctaaa agattcaact gactatgcta aatcaatggc
     8821 actaaaagac gaaaaagttg cacgcatgga taaagattta gttgaatttc tgatggatga
     8881 tacagaaaga ttaaaagggg atcagttttt gagtttaatg aaagacaaag gcttaaacac
     8941 atatggttta tttgattata agaaattagc tatagagtgg gtaaaagaag gagaaatatt
     9001 ttttattgaa gaatgggaag gtactgagaa cttagttctt catcagtatc ttttgaaagc
     9061 ttaatctaag taaaacagtt ctgactagtt atgcttgtca gaatttcaaa ggatgttttc
     9121 cataaccatc atcattcaaa aataaaggat ttgaaaataa gggttccttt gggaaatcta
     9181 aagatgaagt taagggtgtt gtgatagacg atgatttcaa gtttgatttt gactagattt
     9241 ttttgcaagg tttaagagta aacaaggtaa gaactaagat catgaagatt gagagtaaag
     9301 aagatagagg acgaatgtat gagaacacac tatatttatt tagaagataa aattatagat
     9361 gaaattccgg caaaaagtcg agaacaagct agaaaattat ttattgaaaa atacaaagag
     9421 atcaatggag atagtgaagt tccaaaagtg gtcgtaacag ttagaaaaag gcaaacaaaa
     9481 aagagaagta cgtttcatgt cagccctgat atatataatc ttatagagcg acatagaagt
     9541 gaagataata tttctatctc tcaatatatt gctacagtta ttgatgaatt gatttcagga
     9601 aagtatgact atgcttttga ttttgaggat gaaaaggatt acaaaagtgt tgctgtcttt
     9661 caatctgatt ttgagaagct aaaaaactat gctggcagga gaaatgttaa ttttgcaaga
     9721 ctactagaag agttctactc cgaagagttg ggagtaggaa atgaatacaa gagagaaact
     9781 tatgcgaaat tgtctcataa gttatcccaa gcgcttaaag aagtgacaga agttaaagcg
     9841 gaactagatg ctaaacttga agaagtgaaa aaatatcaaa cagagaggaa gaacaacatg
     9901 aagaatcaag tggaatttga cgaacagaaa gaatttgaaa aagcaagaaa acgctataac
     9961 aaaattatga atgaacttag ctatgaacac ttaacagttc atgaagaatt gagtgagggt
    10021 acagaaaaat atacgatcgc agatatggtt aatgaagtgg aaactctttt agcttctttc
    10081 tttgaaaatg gtcacgtaaa cgatgaatgg cgaaaagaag aacctaaaag ggttaacgct
    10141 tatattgctc gattaaaaag gtttattgat aaatataagg agaatagtga caaattttct
    10201 aaagataaat aaggagagag tgtgattaca tttgccagaa atcgaaggaa tattttcgcc
    10261 aaaaataggt aaaaaacagt tagatttcag cagttttccg agcgtaaata gagattataa
    10321 tctaggtgat gaggtacaga tactaaatat tgatttcaaa gaactagact tcgctgttgc
    10381 tgtttccaac aaacccaagg cattggaacg agtgaaaaaa atataccaag agaatgatca
    10441 aaatatcaaa aatatgatca aaaagtatag acgagataat tcaaaagttg aagacgtttt
    10501 attcaacggt acggcaaaac atgatttgaa tgtttatatg gcattaggag tggctttgta
    10561 ctgtaattca attaatcaga cagatatttt ggttaatatc gtgaggaata gctatcaaaa
    10621 aatattaaaa ataagtagcg aaacaaaccc ttattatgca atgaaatatc ttggggataa
    10681 tttattaaat gataaaaaat tgatgcacaa cactgaagag ctgttatcta atttggctgc
    10741 gattaacttt ctattcaaaa caggttctga agattatttt atagataacg tactaaaagg
    10801 tgcttacgga acagcatttt atccgcttta caatatgaaa cagcataaag ccgaacaaaa
    10861 ggataacaaa cttgaaatta aaaagatgaa agaactatta ggtataggat ttgtaaagcc
    10921 caattcaaaa gtcactattg cttcgttagc aactactaac aataatggga aaatagtaaa
    10981 tgggtttgac gattcagata tagatatatt gtcaaaatca tatttttctt tgtatggaga
    11041 ttttgcagat ttgatgggta ttgatagtta cttagtcaat aattttgaga tcaagggtag
    11101 cgagctgaat gatattgtgt atttcgtttt aatctctcta tatgcaattg atcagaatac
    11161 cgagtaccga caaattgact atttgctagt tttttatagt ttgttatatc agttcgtttt
    11221 aactcggttg tttaaaaatg ctaaggaaga attgatcctg aattctgatt atgaaatagc
    11281 aaaacaaatt gcaagtatta gaaaagaaaa caaatctgaa gtaatcaaat taagaaaaga
    11341 attaagtatt gcgagtaaag aaaacggtcg gttagaatct ttagtcaaat cggcaaaaaa
    11401 agaagaggaa cttttgaagc aagagattaa agaattaaaa ggaatcagaa aagaacttga
    11461 acagctgagg tttgaaaata aagtattaaa gcaattgcag gagcaagaac atagacaatc
    11521 attaaatggt aaaactgaag agccttcact gtctgaaatg atagcttatc taaaacataa
    11581 aaacattctt ttagtgggag gacatgtaaa ttttcaaaat aaattaactg aactattacc
    11641 aaatattaaa atgtttgatg taagcgaaaa taatggaaag atacttaaaa atttgaatac
    11701 gtgtgatgcg atttttctat atacagatta cttgaatcat ggtctatatt atcgtgttat
    11761 gagtgaaatt tctaataaag ataagatatt ttatttagat aatcatgtga atatgagctt
    11821 aacaattaaa cgtatttatg atcttttata tgtttataaa cattaatgag gtgttaaaat
    11881 tgacaacaat aactatgaca aaaaaaatag aagtatatgt tgatggatca caagaagaaa
    11941 gggattcctt ttttgatttt ttttggaaaa atagtagaga atatgtaaaa gcgttgaatc
    12001 tggtatataa acagctaatg tttgaagagt tgataaaaga tgcaattaaa gaaatggatc
    12061 aaggttatgc tgagagagaa gaaagtattc aaaatcggat tgaaaagtta gcatatactg
    12121 atccaataga tcaaaaaaaa cttgataaag caaaagctga attatctaaa ttgagaaatg
    12181 aaaaaaataa agaagcgaga gaaatattaa cgcaagcgat agggttgaag agtcaaacga
    12241 gaacacgaga tgtagcaaaa aaattagact ttgactttgg agattttgtt gattccgcaa
    12301 atatgaaagc tagtaaagat tttggcaatg attttatcgg aattgtgacc ggacaaagaa
    12361 cgcctagaat atataaaaat agaagtaaat atggattatt accttttaga ggtcgagata
    12421 ttaaaatcca taaagatgat aagttttata tttatttacc taaaggcttt aggtttaaag
    12481 tgaatttagg tagcaagcca agaaaagcaa gagatgttgc ttcagtttta caacacatta
    12541 tggatggcga ttacaaatta aatcaaagta atcttatttt tgatggaaga aaattatttt
    12601 ttaatctaac aatttctttt gaaaaaagat ataatgaact ggagttcaag gaaggtaatg
    12661 aattgatcgt aaactttgca gatttgtgta gcggtgaatg tttagttaat aataaatcgg
    12721 taatgtcttt tggtgatagc caatatataa gaaacttcaa aagtaaaatt ttagcaatga
    12781 aatcaaaaga acaaagccga ggagtctttt ctaaaggcgg tcatggtcga aaaagaaaat
    12841 tagatcaaga tcgatgggaa gcaataaaga aaagagaaag taattttgtt aaaacttata
    12901 ataatcaatg ttctgcaaaa atcgttaaaa ctgctattcg caataaagct caaattattc
    12961 gtatcattga accagataag gcttatggcg aatgggctta ctatcaattg aaagaacaac
    13021 tagtaaataa aggaaaaaga tatgggatag aagtcagaag ttagtaaagt aaacaatcgt
    13081 tgatttggca acgaaatagt gagggaaaat ccactcactg aagggtaaac tccgtcaagt
    13141 tgtgtgacca gacggaagga cgagcgttgt tggggtttta gaaccatata aaacagcatg
    13201 tttctcaaat tgtgtttgtt tcatttatgt tataataaga gttttagagc catataaaac
    13261 agcatgtttc tcaaatgagt actttaaatt aattgatggc gttcctgctt tagaaccata
    13321 taa
//
LOCUS       DAZUKC010000067        13307 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000067 DAZUKC010000000
VERSION     DAZUKC010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.068"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(240..312)
                     /locus_tag="ACG80T_001993"
     tRNA            complement(240..312)
                     /locus_tag="ACG80T_001993"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(277..279),aa:Lys,seq:ctt)
     gene            693..1262
                     /locus_tag="ACG80T_001994"
     CDS             693..1262
                     /locus_tag="ACG80T_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFS4841628.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1249..2109
                     /locus_tag="ACG80T_001995"
     CDS             1249..2109
                     /locus_tag="ACG80T_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS4841629.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2299..3003
                     /gene="yycF"
                     /locus_tag="ACG80T_001996"
     CDS             2299..3003
                     /gene="yycF"
                     /locus_tag="ACG80T_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFS4841630.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            3008..4843
                     /gene="walK"
                     /locus_tag="ACG80T_001997"
     CDS             3008..4843
                     /gene="walK"
                     /locus_tag="ACG80T_001997"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFS4841631.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4840..6156
                     /gene="yycH"
                     /locus_tag="ACG80T_001998"
     CDS             4840..6156
                     /gene="yycH"
                     /locus_tag="ACG80T_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFS4841632.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            6157..7026
                     /locus_tag="ACG80T_001999"
     CDS             6157..7026
                     /locus_tag="ACG80T_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFS4841633.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            7081..7890
                     /locus_tag="ACG80T_002000"
     CDS             7081..7890
                     /locus_tag="ACG80T_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS4841634.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7993..8622)
                     /locus_tag="ACG80T_002001"
     CDS             complement(7993..8622)
                     /locus_tag="ACG80T_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS4841635.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8792..10084
                     /locus_tag="ACG80T_002002"
     CDS             8792..10084
                     /locus_tag="ACG80T_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4841636.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10160..10822)
                     /locus_tag="ACG80T_002003"
     CDS             complement(10160..10822)
                     /locus_tag="ACG80T_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFS4841637.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            11047..11331
                     /gene="groES"
                     /locus_tag="ACG80T_002004"
     CDS             11047..11331
                     /gene="groES"
                     /locus_tag="ACG80T_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFS4841638.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11382..13007
                     /gene="groL"
                     /locus_tag="ACG80T_002005"
     CDS             11382..13007
                     /gene="groL"
                     /locus_tag="ACG80T_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFS4841639.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4425 a         2288 c         2856 g         3738 t
ORIGIN      
        1 atgtttagag agttcattgg cagcatgatt ttgcaaagct tctcgaatta ttgttatatt
       61 agaaacttta ctgttcataa ttatgcttct tttttaactc aaagtccaaa gggctacagt
      121 ggactttgag ttaaaaataa aaagccctag ttattttgaa aaataaaaaa agaacagcgt
      181 aaatgttttc gaaatgcttt cgcaccaaca tttacctgtt ccgtgggttt ggcacccaat
      241 gggccgtgag gggctcgaac ccgcgacccg ctgattaaga gtcagctgct ctaccaactg
      301 agctaacggc ccgtacaaaa agtacttaat aatagtagca ctaagaattt taaaatgcaa
      361 gactacattt aaagaaaatc agaaaaatct ttacttcctt ataggaaatc attcatagat
      421 tattcacatt tgctcgctat tatataagca taccaacaaa caaagttctt gcaagagaac
      481 aaaccctaac acttttttca ttttttactc ctttcccgcc gtctccccgg cgggtctcct
      541 gttcttaacg ataaaagaca agaaggctta gccttcttgt tttttgtttt catagaatcc
      601 cgctaatcaa atcacctagt taaagcaact agattgaatg tttatcataa gtctgatatc
      661 atgtttatga aatgaaaaaa ggagcggtta tgatggaaaa aatgcgtcag caattaatgg
      721 agtgggggaa ggaactagaa acattccgtc tgcctcactg ggaagaactc cctgacttag
      781 aactttatat ggaccaagtc agaacgttag ttgatcgtta tctttcacct gtgatacaag
      841 gagaaaagca tccattgctt acctcttcta tggtaaacaa ttacgttaaa ctaggtttga
      901 taccagcacc agttaaaaaa agatataaca aagaacacgt agcattttta cttgcaatca
      961 ctacgttgaa acaagtattg actattcctg aaatcaaaga agggatattg ttccaaggaa
     1021 aaacagtagg aattcgtgaa gcctataatc tattttgcga tgaacaagaa gcagctgtgt
     1081 ggatggtcag ccaattagca caaggtaaat ctcatccgca aaaattcgac caggcaaccc
     1141 ctgtagaata tattgctgta agagcggcaa ctttatcgtt tgcaatgaaa cttcttgcgg
     1201 aaaaaacgat tgttctagaa accgaatatt taaaagagga gaaaacaaat gaaaaacaat
     1261 aaaatcgcaa ttttagtaga ttcaggtacg gatgttccag aagaattgat aaaaaaatac
     1321 gagatgtata tgatcccatt gaaaattatt tataaagatc aggtatatac agataaaatt
     1381 gacattaccc ctgaagagat ctatcaacgt ttgccagagg aaatccctgg tacatctttg
     1441 ccggatggtg agtcgatcac agaaatcttt gagcaaatca aaaaagacgg ttatgaaaag
     1501 gtacttgcgg taacaatttc cagtggattg agcgggacat ataatgtggt tcgtttaatt
     1561 gcacaacagc aagaagaatt ggaagcatat gtactagata cgaaaaatat tggtattggt
     1621 gcaggttttt ctgcgatcca agcagcaaaa tggctggaag aaggagtaga atgggatcaa
     1681 ctgatcagta atctgaatga gttagtcgaa agaacaaaag ttttctttaa tgttgcgaca
     1741 ttggaatatc ttcaaaaagg tggacggatc ggtttagttg cttctattgt aggaaccgct
     1801 ttaaagctta acccaatcat ctcatgtaat gaagaaggga tttattacac agtaggaaaa
     1861 gctaggggac gtaaaaaaag tttggataaa acggttgctt acgtcaaaga aaaaatcggt
     1921 aatgcaaagg tcttcaactt agctgttgcc catggggatg cgaaagaaga agcaattgaa
     1981 atggaagcga gacttaaaag agagttccca caagcaaacc aaatctactt tggccagatt
     2041 tctccggcat tagtagtcca cactggtccg ggattattgg gtgtcggtgt acaagtttta
     2101 gaaggataaa tcaaaaaaag gaaacactga ggtgaaaatc agtgttttcc ttttttattg
     2161 aagttagaac aaaaaataat aaagtatttt gcactttcat tttaattttc tcttttgttt
     2221 tataggcaaa gatgaaacaa aagcagaaat acggtataat gagaaggtat gacaataata
     2281 tataagaggt ggctctttat gaaaaaagtt ttagttgtcg atgatgagaa gccgatttcg
     2341 gatatcgtca aattcaattt agccaaagaa ggctatgacg tttatactgc atatgatggg
     2401 gaagaagcct tggaaaaagt tgctgaggta gaacctgatt tgattttact tgatttgatg
     2461 ctacctaaaa tggacggctt agaagttgct cgtgaggtac gcaaaactta tgatatgccg
     2521 atcattatgg taacagccaa agattctgaa attgataaag tcctaggctt agagttaggc
     2581 gccgatgatt acgtgaccaa gccattttcc aaccgagaat tagttgcacg tgtcaaagca
     2641 aatcttcgtc gcggtgccac ggctgcgaag gaaccagaag aggcagcacc tgcggaattg
     2701 acgattggtg atttgacgat ccatccagaa gcttacatgg tgacgaaacg aggagaaaca
     2761 atcgagttaa cacatcgtga attcgaacta ctcttttact tggcgaagca ccttggacaa
     2821 gtgatgacaa gagaacattt attacaaacg gtttggggtt atgattattt tggagatgta
     2881 cgaacagtgg atgtaactgt acgtcgtttg cgtgaaaaga tcgaagataa tccaagccat
     2941 ccaaattatt tagtgacacg tcgtggtgtc ggttactatt tgcgtaaccc agaacaggag
     3001 taattggatg aaaagaaaag tccgcttttt tcgatcagtc aattttaaga tcgctattac
     3061 ctttattctg attttattga tctcgatcga aattattggt gcctacttta tacgtggttt
     3121 agaacgttcc acgatcaata catttattaa agatatgaat caaacggtgg aatcattagc
     3181 aacaacaatc agcccggaat tgaatcgaaa agataatgca gatgatgaag aggtaaatgc
     3241 gaatatcaaa agatttattg aaaacagtgc gactagcgat atcatcgaaa tacgggtcgt
     3301 tgacgaaaaa gggatcatcc gaggaacaac cgatgtcaat gaacaaagcg ctgtgggaaa
     3361 gaaaaatgat tatgttgata tcaatgattt cacgactaaa cgttatgttg ctttagataa
     3421 cgacgataaa cgggtgaata tcaatgttca accgatcctg tccccgaccg gcgatgccgt
     3481 tatcggggct ctttacgtca aaagtaatat cgaacaaaaa tattcggaaa tcaataacac
     3541 agcagtcatt ttctttactg cctcactaat tgcggcattt atttcaatga tcgtatctgt
     3601 tttagttgct cgttcgatta cacagccgat cggtgaaatg agagaacaag ccattcgaat
     3661 cgctagagga gactacagtc ggaaagtcaa agtgcatgga caagatgaac ttggacagct
     3721 ggctgatacc ttcaatcagt tagctgaacg tattgaagaa gctcaagata caatggaagc
     3781 tgaacgcaat cgtttggata gcgtgctatc ccatatgaca gacggcgtca ttgcgacaga
     3841 tcgcaggggt aaagtcatta cgatcaatga tatggctgtt tccttgctag atatcaaaaa
     3901 cgaggaagca atcggacaat cgattttgac acttttagat atcgaagaag aatatacgtt
     3961 gcggaaatta ttagaagatc caaacgaaat ggtacttgac cgttcaacca gtacattgga
     4021 aagtgatcag atgatcttgc gagtagactt ttctatgatc cgtcgcgaat caggttttat
     4081 cagcggatta gtagctgtct tgcatgatgt gacagaacaa gaaaaaacag aacgggaacg
     4141 tcgtgaattc gtctctaatg tttcccatga acttcgtaca ccgttgacaa gtatgcgtag
     4201 ttatatcgaa gcattgagcg aaggtgcgtg gaaagacgag gaagtagcac caaatttttt
     4261 gaaggtaacg ctagatgaaa cagaccgaat gattcgaatg atcaatgacc tcttaaatct
     4321 atctcgcatg gatacaggta atacccagct tcaattagaa tatgtcaatt tcaacgagtt
     4381 agtgaatttt gttcttgacc ggtttgatat gatggtggga aatcaggaaa aaaattataa
     4441 gatccgtcgt gaatttacgc aaagagatct ttgggtcgaa gtagatacag ataaaatcat
     4501 ccaagtagtc gataatatca tgaataacgc gatcaagtat tcaccagatg ggggaacgat
     4561 tacttgtcgc ttattagaaa cgcataataa cgtgatttta agtatcaccg atcaaggatt
     4621 ggggattcct aaaaaagatc tgaaccgagt gttcgagcgg ttctatcgag tagataaagc
     4681 acgtgcaaga gcacaaggtg ggacaggact tggattagct atttctagag aagtgattaa
     4741 ggcgcatcga ggcgctattt gggcagagag caaggaagga aaagggtcga ctttttatat
     4801 ctcgctgcca tatgaacctt atgaggagga atggtgggaa tgagaatctc tgaaaaagtc
     4861 gttcgtatag gactcatttt gatggttgcg ttaagcatat acttctctta tgcaatctgg
     4921 ctgagcccag caggaaaaag ttcaatcaac gtagatgaga caaacactca aatgatcgag
     4981 tctcaaagca atcgaaaaac ttccgaagtc tttcttccgc ttcatgttac atggcaccat
     5041 tcgggaatga tccaagaaac caacagtgaa aatctcgtgt accgattgca attgatcatc
     5101 gataaagcaa gatttggaaa gctttctgaa gtagtttcag gagacaaatc acagtttgaa
     5161 caggtgaaaa atctgaactc tggttttgag ttggcctata atgcgccttt tttgctaaga
     5221 gaatacaagg aagcatttga tttagattta gatgtgtcga acttggcaaa agaaagcgaa
     5281 aatatgtatt ttactagaat acaattcgat caaggaaata aaaaaatccg atttttgaat
     5341 tattcgaacc atctcgtata tgaagcaaat atttcgttga attggacaga tatcaataaa
     5401 gaattaacag caaaagacac aaaatggaca gaaatgacaa cagaaccatc gacgatcgac
     5461 acgcaatatg ctacgaaaca ttcgatcaaa ctgaagaagt atagttatat cctgtcacag
     5521 caaccctata cactttttag aaatgctttt ttccagaaac cagatagcgt gaaaaataat
     5581 gatgaaagca atgaattgat tttttacggt gggaatgaaa caatgtctat ccattcggaa
     5641 acgcaagtag ttgattttcg tggagagctg ccagagaaaa aagaaaatgc tagtgtattt
     5701 tctgaaagtt tcccctatat tagcaaatta ggcagttctt tagggaacct cagatatttt
     5761 gaccgcagtg acaatacgat cgattatcgg atattcgtgg aaggcttccc ggtttttggg
     5821 acagacagca gaggacagat tggtgtggaa gttggcggtg aaacgaatgg ttctgtgcaa
     5881 gtgaatatcc aaaccagttt gaatacgatt caagtgccga taccttcaga cgaagaagta
     5941 gaacttgcaa gtacgaatga aatcatccag cagttaaaag ctaagggagc agatagcaac
     6001 aagattggtt cagtgatcgt tggctataca tggcaaaatg tcaaagaaac gaatcgtgtc
     6061 gttgatctct tacctgaatg gtatgtgaaa tataaagata actggtattc agctaacgac
     6121 cttctggaaa aattacctgg attggaggcg aattaattgg atttcaaaaa aatcgaatgg
     6181 atatttttcg tcgctttttt agggttgaat gtgtttttat tcagcagtta tcatgacgcg
     6241 gtataccaag aaaacaatgt cattcgctct aatcagactg aatcaatcca acaacgtctg
     6301 gctagcgacg atatcactta cacggggaag atttcgtctg agaacaagca aggctattat
     6361 cttagcgcgg agcagaccaa tcttagcact tcattagcga attatcgcaa ggctggtaaa
     6421 agcggcttgc tggcaagtgg tgtgttttta gacggtgatg ttttgacgaa atctttatcc
     6481 agttcagatg ctgaaaagaa cgtgatcgat ccagataaaa ttagtagttc cttggaagat
     6541 tttttatcta acaaagacaa tattttattc ggtaatgatt atgtgtacac gaaaaacttt
     6601 tctcatttag aagaagaacc actagaaatc caagcaagcc aagagtatga aggcatccca
     6661 ttcaatgatg atacagcaaa aatcgttctt tctttagaaa aaaacgataa tgattatagc
     6721 atcacgaaat acatgcagac gcatttatct gatatcgagc agctgcgcga aaagacggaa
     6781 ctgtatacgg aagaggaagc aatcaataca ttgtacatca ataacaaaat accaagagga
     6841 tcaaaaattt tatggcgtca gctagcttat tcctgtattc tgaaagtccg ggaaaaaaat
     6901 gtgtatgttc ctgtttggca tgtagcgatc gaaacaagcg ataaagtggt ccaaattgag
     6961 agcgtcaacg ctttcagcaa tacgatcgtg acaaacaata ctgtaccaaa agtggaagat
     7021 caataaaaaa gcgagtataa tgaaaaaagt ttacaaagta agaagggaag taggagtcaa
     7081 atggctgaag aaaaaccagc ttttaaaatc agtatcctag caagtggaag ttcgggtaat
     7141 tcactttata tcgaaagtga aaaaaaacgg cttttggtcg atgcaggact tagcggtaaa
     7201 aaaatcactt ccttgatggc tcaaattgat cggacaccgg accaattaga tggaatactt
     7261 gtgacgcatg aacatcgaga tcatatacat ggcgtgggtg tcttggctcg taagtatcat
     7321 ttggatgttt ttgccaatca gcagacttgg gaagcgatgg aaccactgat tggaaatgta
     7381 ccagtggaac aaaaacatct atttgagatg gggaaagtca gaacattcgg cgatatcgat
     7441 attgagagtt tcggcgtctc ccatgacgca gcggctccgc aattttatcg tttccataaa
     7501 gaaaatcggt cattcgtcat gttgacggac accggatatt gcagtgatta tatgcgaggt
     7561 gtcattgaaa atgccgatgg ttatttgatg gaaagtaatc atgatcttga gatgttacgc
     7621 atgggacctt atccgtggaa tttaaaacag cgtattttag gagatagagg acatctttcc
     7681 aacgaagacg gtgcacttgt catgactgat gtgatcggta atcggaccaa acgaatctat
     7741 cttggccatc ttagcaagga aaacaatatg aaagaacttg ctcatttaac aatggaaaat
     7801 gttttgaaag aaaaagattt tggcgttgga catgattttg aaatctatga tacagatcca
     7861 gatgaagcaa ctgagctatt ttcgatttaa acagttcaat caataaaaat ataaaaagca
     7921 gaagataaaa tcaatcaggg ttggttttat cttctatttt tattttttta gcatattaaa
     7981 aaaataaccg tattatcgac tgactacact gatgtgctgc ttttcatgtt tctcatccag
     8041 tatctcatct actaatgcaa tcgcgtaatc tgccatgctg atcgtactgt cgctagcagc
     8101 gtttctttgt aaataatcat cacttaacaa ataagtacct gtacgtttgc catgtggatc
     8161 aaataaagaa gctggactga tataagtcca atgtagattt ttagattgtt tcaattttaa
     8221 caatccttcg tacatattga aagctgttgg atagaagggt gcatttttag gtacttggtc
     8281 aatcatacga gtcgtctttt ctgtatctaa gaataacgaa gaggcacccc caacgacaat
     8341 taagcgtgta tcggttcctt caagcatctc aatcaaatgg gacaaagaag tttgatgcat
     8401 ttcttctttt cctttgggag cgttgaaagc atcaacgaca acatcaaaag atgcaagatc
     8461 agtagttgtc aactcaaata aatccttttg tataaacgga acatctgatg gcaatttttc
     8521 tggatgacgc acgattgccg tcacgtctaa atctctggac aatgcttcgt ctaaaataaa
     8581 tgaacctgca tggccagtag ctccaatgag tgcaattttc ataaaaaacc tcctttttta
     8641 ttcctgtcca ttgtaagcag ttgctgaata ttcgtcaaag aatccgattt ttgaaaagct
     8701 cagtgcgcat tcctttcatg gttcgtgatc attgggtata gttaaattaa gaaaactgat
     8761 tgataaaaat agaagttgaa ggagataaga aatgaaagag tggttagatc gtgccttttt
     8821 accaatgata acatctgttg tgaatcttag acccttaact gctttgcgta atggaatgac
     8881 ttacacgatc ccaataatta ttgttggctc gctgtttttc ttatttgctc attttcctgt
     8941 ggattacatc gctcagtgga tagacaaaac aggattttct atgtatttta cacaagtata
     9001 ccaggctaca tttgatgtga tagccttttg gatcgttttt gccattgcat acgcttatgc
     9061 aaaagaagaa gacatcgatc gcttaccagt cggcataaca gccttgtctg cttatctact
     9121 gcttattcag ccactaaata ctcagataga tactgtctgg acaggtgctg aagggatact
     9181 aggagcagtt acaactgggc tcatcatcgg atggggctat gcacgtttag taagacttgc
     9241 gaaagggtgg aagcaaagag agaagatacc tgaaaatgtt tggcagtctt ttacttcatt
     9301 gattccaatc attgttatag tcacttttac gcttatcggt tcagcggttt ttcaacattt
     9361 gttcagaatg actgttgttc aaggagtatg gacatatgtc cagcttccca ttcaatcgtt
     9421 catagacacg ttgtttggtg tgcttttatt aggttttttt gttccgtttt tatggttatt
     9481 tggtcttcat ggatctgcga tggtcaatgg gttggttagt cccgtcttgc aagcgaattc
     9541 attagcaaat gcagaaatct tagcctcagg acaagagttg acggtggcaa atggcggaca
     9601 tatcgttact cagcaatttc tcgatcagtt catgacggtt acgggtgctg gattgacgtt
     9661 aggggctgta ttttttatga cggtttttgc gaaatcgaag aaatacagag aattaggaaa
     9721 tcttgctctt ttaccagcct ttttcaacat caatgaatca attatttttt ccacaccgat
     9781 cgtgatgaac ccgatgatgg ctgttccttt tattttcgca ccggtcgttt caggattgat
     9841 tacctattcc gctttatatt ttggattcgt tcctttattt acagcagtcc aagtcccttg
     9901 gacaacaccg ccgatcctct cgggtttctt agtaggcgga gtgcctgcag cgattttaca
     9961 aggtatcgtg ttgtcaatta gttttttcat ttatttccca tttttgaaaa aaatagattc
    10021 tgcaaactgc aaaaaggaaa gacagacaaa aaatgatgga gcagcagaaa agatgaacga
    10081 ctaaaaaaca aaaatactcc tgtcgcttta gattgggaaa atcaccaatc taggatgaca
    10141 ggagtattta tagtcatgtt tatactagcc ctttttggac agctaattga acaagaacaa
    10201 ctaatgtgtt cattcctacg tgtgtgatca tcgatgtcca gatccgacca gttgctttgt
    10261 actctaggct gaagaagaaa cctaggaaaa agtagattag cagatgacca tcattatgag
    10321 caaacgcaaa cagcaaagag ctgatcactg ctccgaccca aaaatttgaa tatcgactga
    10381 tgatccccag tatactgcgg cggaatacga actcttccat gatgggtcca gcaacagttg
    10441 tcgccaaaat aaaagcgggt gcttccatga tcatctggat gatattttgt gtattctcgg
    10501 aaggggttgc tttgccaaac aatacagatt ctaacatgac agcgatgctt tgcagaatca
    10561 atgcgacaaa gattccaacc aatccataaa taacaatctt tggagcagaa gcttttttta
    10621 ccgctgattc gatcgctaag ggttcacgtt gcttaaaata aaaaatagct aggacaacgg
    10681 cgcctagtat atacatgact gcagttgctg tgatcaattc agacgtagat tttacaagtc
    10741 ctatcgaagc taggatcaag ggtgagataa aaattagacc atagcaaaaa atgctgagga
    10801 agctatattt ttttaaagac atgtaattcc tccttttaag ggataacaag catgcaaggc
    10861 aagcttatat cgattgattt caattatatc ataatttcaa taagcagctg tcagaataaa
    10921 atttcttttc tttgatttaa aagcttgcaa aaaagaggga gaatgagtat tattaaaaat
    10981 gtaggttagc actcgaaggt taagagtgct aacagattaa aaaaagagtt atcggaggga
    11041 tttatcgtgt taaaaccatt aggtgatcgc gtcattatcg aagtcgcaaa agaagaagaa
    11101 aaaactgtcg gaggaatcgt tttagcttct gctgctaaag agaaacccca aactgggaca
    11161 gttgttgcag tcggtgaagg ccgtttgtta gaaaacggtg aaaaagttcc tgccgctgta
    11221 aaagcaggcg accaagttat gttcgaaaaa tacgccggta cagaagtaaa atacgaagga
    11281 aaagaatatc tgatcgtcgc aggaaaagat atcatggcga ttgttgagta aatttggaag
    11341 ataaaaaaat aatcaaaaat aatcaatgag gtgaatcaat catggcaaaa gaattgaaat
    11401 ttgcagaaga cgcacgcgca gctatgttac gcggggtaga taaattagca gatacagtga
    11461 aagtaacatt aggacctaaa ggccgaaatg tcgtactaga aaaatcttac ggctctccat
    11521 tgatcacaaa tgatggtgtg acgatcgcaa aagaaatcga attagaagat cattttgaaa
    11581 acatgggtgc aaaacttgta tcagaagtag catcaaaaac aaatgatatt gcaggagacg
    11641 gtacaacgac agcaactgtt ctgacacaag caatcgttcg cgaaggttta aaaaacgtaa
    11701 ctgcaggtgc taatcctcta ggcattcgtc gcgggatcga attggcaaca aaagcagcag
    11761 tcgaagaatt gcataatatc tcaactgttg ttgattcaaa agaagccatt gcacaagtag
    11821 cagctgtttc ttcgggttct gataaagttg gacatttgat tgctgatgca atggaaaaag
    11881 ttggaaacga cggtgtcatc acaatcgaag aatcaaaagg gatcgaaaca gaactagatg
    11941 ttgtagaagg tatgcagttt gatcgtggtt acttatcaca atatatggta acagataacg
    12001 acaaaatgga agctgttcta gaaaacccat atatcttgat cacagacaaa aaaatctcta
    12061 acatccaaga tattctgcca ttgttagaac aaatcttgca acaatcacgt ccattgttga
    12121 ttattgctga cgatgtagat ggtgaagcat tgccaactct tgtattgaac aaaatccgtg
    12181 gaacattcaa cgtagtagct gtaaaagctc ctggtttcgg cgatcgtcgt aaagcaatgc
    12241 ttgaagatat cgctattttg acaggtggta cagtaattac tgacgatcta ggtttagaat
    12301 tgaaagatgc aacaatcgaa aacttaggaa atgcttctaa agttgttgta gataaagaca
    12361 atacaacgat cgtagaaggt tctggagaaa aagaagcaat cgaagctcgt gtgcaattga
    12421 tcaaaaacca aattgctgaa acaacttctg attttgaccg cgaaaaatta caagaacgct
    12481 tagctaaatt agcaggtggt gtagctgttg ttaaagttgg tgcagctact gaaacagaat
    12541 tgaaagaatt aaaattacgc attgaagatg cattgaatgc tacacgtgcc gcagtagaag
    12601 aaggaatggt ctcaggtggt ggtacagctc tagtgaacgt tatcagcaaa gtttctgctg
    12661 tagaagcaga aggagacgtg gcaacaggta tcaagatcgt tgttcgtgct ttagaagaac
    12721 caattcgtca aatcgctgaa aacgctggtt atgaaggatc agtgattgtt gataaattga
    12781 agaacgtgga actaggcact ggtttcaatg ctgctactgg agaatgggta aacatggttg
    12841 aagcaggtat cgtagaccca actaaagtaa cacgttctgc cttgcaaaat gccgcttccg
    12901 tttctgcctt gttattaacg acagaagcag tcgttgcaga taaaccagaa ccagcagcgc
    12961 cagcagctcc tgcaatggat ccatcaatga tgggcggtat gatgtaagaa aaagcaactt
    13021 gcttatctac aactgatcat tagtgaaaga cagataaaaa gaggctggaa cagaagcgtt
    13081 taactccaag aaataagaag aaattcacgg aaattgcttt tcaaattttt gtgaatttca
    13141 gcttatttcc gaaggagttg cttctgcttc cgctgtttat acgtgtttag agcgtgagac
    13201 aaaagtgttc tttacttttg tctcacgctc ttttttgtgt gtaattataa tgaaaatagt
    13261 tatttttaat agttataata ttttatctat taaaagtaaa tatccat
//
LOCUS       DAZUKC010000068        13104 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000068 DAZUKC010000000
VERSION     DAZUKC010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.069"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            69..1808
                     /gene="yfcC"
                     /locus_tag="ACG80T_002006"
     CDS             69..1808
                     /gene="yfcC"
                     /locus_tag="ACG80T_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFS4841640.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1965..3149
                     /gene="iadA"
                     /locus_tag="ACG80T_002007"
     CDS             1965..3149
                     /gene="iadA"
                     /locus_tag="ACG80T_002007"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFS4841641.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3260..3463
                     /locus_tag="ACG80T_002008"
     CDS             3260..3463
                     /locus_tag="ACG80T_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFS4841642.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3564..4226)
                     /locus_tag="ACG80T_002009"
     CDS             complement(3564..4226)
                     /locus_tag="ACG80T_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4841643.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4239..5741)
                     /locus_tag="ACG80T_002010"
     CDS             complement(4239..5741)
                     /locus_tag="ACG80T_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4841644.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVTVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6289..7968
                     /locus_tag="ACG80T_002011"
     CDS             6289..7968
                     /locus_tag="ACG80T_002011"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFS4841645.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8095..8574
                     /locus_tag="ACG80T_002012"
     CDS             8095..8574
                     /locus_tag="ACG80T_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFS4841646.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8793..9473
                     /locus_tag="ACG80T_002013"
     CDS             8793..9473
                     /locus_tag="ACG80T_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFS4841647.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9481..10812
                     /locus_tag="ACG80T_002014"
     CDS             9481..10812
                     /locus_tag="ACG80T_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFS4841648.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10827..11789
                     /locus_tag="ACG80T_002015"
     CDS             10827..11789
                     /locus_tag="ACG80T_002015"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFS4841649.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11900..12238
                     /locus_tag="ACG80T_002016"
     CDS             11900..12238
                     /locus_tag="ACG80T_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFS4841650.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12317..13024
                     /locus_tag="ACG80T_002017"
     CDS             12317..13024
                     /locus_tag="ACG80T_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFS4841651.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4162 a         2316 c         2878 g         3748 t
ORIGIN      
        1 gtataattca tttgattatc cattttttat tcatcaaaat tactgaaaaa agaaaggaag
       61 aaataaattt gaagaaaaca aattcttcag gaaaaccgaa acgcgacttc actaaattat
      121 cgacacccca tacgtatgtc attatttttg gtgtagtcat ctttgcatgg atactgactt
      181 ttgtcgttcc agcaggaaag ttcagcacgc aagatattga atacaaagat gcaaatggcg
      241 aaacaagtac tcgtactgtt ttacgtcaag attccttccg ttatgcttac gagctggata
      301 agtcgtatgt gtttgatcaa ttagaagaac tgcaagatca tccagcagaa cgtgaaaaac
      361 tggatgttcc ggaaaaagga ttggaaaaag tcattgctga cggagaaaaa aatcttacgc
      421 aggaaaaact agatgaaatc tccttgacag atgatgtgct ttacgatcag tacggcgaaa
      481 atatctatga tacttctaaa aaattgcata aaacagccaa aatatgggga acagatgatt
      541 ttggcggctt tggtttctta aattttgttt ttgaaggact agtatctgga gataagtatg
      601 gatctgctgt agggatcgct gcattgatcc ttgttgttgg aggtgctttt ggtattatta
      661 tgcgtaccgg agcgatcgac gcaggtatct atgcgtttat cagtaaaact aagggactgg
      721 agcgtttagc tttaccgcta ttattctttg ctttttcatt cggtggcgct acattcggga
      781 tggcagaaga ggtaattcca ttctcaatgg tgatggttcc atttgtcatt gcgttaggat
      841 atgactcgat cgtagcagta accgtgactt atgtcgcatc tcaagtcgga aatgcgacct
      901 cttggatgag cccgtttagc gtagcggtgg ctcaagggat tgctggaatc cccgtgttgt
      961 ctggtgcgac attccgattg attatgtggg tagttgttac tgcattagcc gctggttata
     1021 tgatgatata tgcagaaaaa atccgtaaaa aaccagaaag ttcgttgact tacaaatcag
     1081 atgattattt ccgttctcat atcaaaaaga catctgatga aaataaaaaa ttcatgcttg
     1141 ggcataaatt gatcttatta gagatgttag ttgtccttgt ctggatcgtt tggggcgtga
     1201 ctcaaaaagg ctattacatt ccagaaatcg cttctcaatt ctttgtgatg ggattagttg
     1261 ctggaatcat tgctgttctt ttcaaattag atggaatgaa agtcaatgac atcgcttctt
     1321 ctttccaatc tggtgcagct gatttagctg gtacagcaat cgtagtaggt atggccaaag
     1381 ggattcttct tgtcttggga ggatctgatg catctgttcc ttcagctttg aatacgattt
     1441 tgcatgggat cgggacggcg ttgaccggtg ttccagctgt cattggtgct tgggcgatgt
     1501 acatcttcca aagtttgttc aacttggttg ttacatcaaa ttcaggacaa gcagctttga
     1561 ctatgccaat catggctcca ctggcagatt tagtaggtgt atccagacaa gtagcagttc
     1621 ttgcttatca gcttggttca ggttttatgg acgcttttac acccgtatca gccagcttga
     1681 ttggtgtgct aggtgttgca cggatcgaat gggcaaaatg ggcaagattc caaatcaaga
     1741 tgcaaggatt cctgtttgtt ttaggaacta ttttcatcat gattgccatt gcaataggtc
     1801 tgcagtagta ctggagacct gtaaataaaa aagtaatcca aacagtagat aaggactcca
     1861 agaaccgtca ttttacggta aaatcaaagg agtaaagatc ctatcgtttg gattattttt
     1921 taagatccta tcatcagtta tacttaaaga aagaggaaaa atgtatgaaa atcattcgac
     1981 aaattgaagt gtttgcacca gattacttag gaaaaatgga tgtactgatt gccggtggga
     2041 agattttggc tgtagaagaa cagctagaag gcggctacga aggagtggaa gtagaagaac
     2101 tttcaggtga gggaaaagta ctaaccccgg gtttcattga ctgtcatttc catattttag
     2161 gtggaggagg agaaggcggt tatcaaaatc gaacgccaga agtcacattg agccaattga
     2221 ctacagctgg tgtaacgaca gttgtcggtt gtttgggaac agacggagaa ggtagagata
     2281 tgactgcgtt gatcagcaaa gcgaaaggtc tggaagcaga aggaatctcc acttatgtat
     2341 acgaaggttc gtaccgattg ccggtaaaaa cagtaacaga ttctatcatc aaagactttt
     2401 tgacaattga taaaattatc ggaatcggag aaatcgcagt atccgatcac cgttcttctc
     2461 agccaagttt tgaagagttc gctcgtgcag tagcagatgc tcgtgtagga ggcatgttat
     2521 ccggaaaagc tgggatcatc aatgtccact taggcggagg aaaaagaaag ctagagctat
     2581 tgacacgaat cgtagaagaa acagaaattc cgatcacaca atttttgcct actcatgcaa
     2641 atcgcacacc agaattattt gaagcttgcg tggcatttgc caaacgtggt ggcacgattg
     2701 acttcactgc tagcgaagat cctgattttt gggagaagac agatggtgaa gttcgcttca
     2761 gcaaagcact gaaacgattg atcgaggaag atgtttcttt agataatttc acgatgacct
     2821 cagatggaca aggaagcttg ccttattttg atgaaaacaa tcatttccta gggttaggag
     2881 taggaagtgc caaggcattg ctagtaggga tcaaagaagc tgtgcaaaaa gaaagtatcc
     2941 ctttggaaat cgccttacga gctataacca gtaatccagc acgtatattg aaattggata
     3001 aaaaaggaaa aatcgaaatt ggtgctgatg cagatctgtg tatcttggat aaagaaacgc
     3061 ttgatattga tacagttatc gctaaaggag aaataatggt ccaagaaaaa gaagtcaaag
     3121 tatgggggac tttcgaaaaa agtttttaat caacagctct aactatcttt tttagaaaac
     3181 gagcgttctg tttgtctgta tctgttgttg atgttaccct gaaggtagat caataaacaa
     3241 actaagagga ggaagcacaa tggcagattt aaaaggacgt tctaaagata tgaaagataa
     3301 agtagtcggt gaaggaaaag aagcatacgg caaagcaaca gacgacaaga gcaaagaaac
     3361 agaagggaaa gcacaatcac taggtgctga catcaaagaa aaagctcgaa atttaggcga
     3421 cgaagtcaaa gacaagtttt ctcgaaaaga cgaaaaaaaa taaaaaccac ttattactaa
     3481 ttgaatcaag caaataaaaa atccgaacca tatctgcgag acagatatgg ttcggatttt
     3541 ttatttgatc attatcctgt tttttaaact tcgatttttt gaaatttttt attttttagt
     3601 tcaaagactt catcacagct ttttgctact tcaatctcat gagtcacgat gataatacag
     3661 cgattttgtt catgggcgat ttcttggaaa agtttgacga tatcttgtga agttgtttca
     3721 tctagatttc cagtaggctc atccgctacg attagttgat gttcacagca gatagcacga
     3781 acgatagcga ctcgctgctg ctgacctccc gaaagtttgg tcacctgttt ttttgctaat
     3841 atttcatcaa ttcctacacg agccagtcga tctaaagcaa cttgtcgata attctcttgc
     3901 ttggtcttag tgatttccat tgctgctatg acgttgtcta gagccgataa ataaggaagc
     3961 aagttgtaag cttggaaaat aatcgagaca tcatttttac gataacttct taacccgatt
     4021 ttgttcagtg aacgatcttg atagtatacc tttccttctt ttggtgcatc tagtccagaa
     4081 ataagcgaaa gaaaagtcgt ttttccagaa ccacttgctc ctaaaatagc gtacattttt
     4141 ccagcttcga aattcagttc tacatcttta taaagcgcat cttgcggatc ttgataccaa
     4201 aatcccaatt gttctgtccg taacatcaaa ttcctccttt agtttaatag gatttttctt
     4261 gggtttaatc ttaatatccc tatagaagct aaaaagacag aaaacagact gattccaaat
     4321 gctactccgg ctaatagtcc caattgtgca ggtttgacat tgatttccaa ttcgctgact
     4381 tgatcagaaa cagcaaatgg attattcgtg gttccttgtt gaccatcgcg ttgaccgcct
     4441 tgttcttgtc tgttacctgg catttgtcca ccgccaggag cttgctggtt ttgtgtcgtg
     4501 gtttctgtct gttggtcaag tagctgctgt cccacggcat tcgcgacgac gtttccgcta
     4561 agcgaagcaa ctcctaatgc aagaatcata caaatagcca cttcggtaaa caattggaga
     4621 atgattttac ttctggattc tcctaaagat agcaagacgc ctaactcatg ccgacgctcc
     4681 cgaatggtaa tcataatgat cagagtaagt atgataatac cggctacagt cactagtaaa
     4741 acaacatttt tagaaaaact tgccacgttg tttaaaggtt ccaacattga ctgatacatg
     4801 gaatcatttg attgtaagct aaatgtatct gtgtcgatca gcttctcagc ttctttgaga
     4861 aactcatcca ttttatctgg atcagataga gtgtaaatag cagaatctat cgtgttatcg
     4921 tcagatgtcc catttagttc atttgcaaaa gtgtaagaag tgtaaagtgt attcgacgga
     4981 ttcatgaagt tgaatttcat acccatactg ccgcttgttt cgctggattc ataaattcct
     5041 ttgatcgtca tttcataagt ggtatcttca tcttcagtac tcgtgattgt gaaagtatct
     5101 ccgactgcca ggtcatttgt ttctgctaga gtagaatcaa tgactacatt gtttgtgcct
     5161 ttgtcgctgt catctattcc ttcaccatcc gtgatttttg ctgttccatc agaaaaatta
     5221 ctattttgtg cagttgctga cacaccactg atttgaaagt ctgcttgagt catttgcgga
     5281 ttcccgccca ttcctccagc aggttcaccg cctgcatcag tagtttctga actgtcttca
     5341 cttgaatcag aactagtgat ggcagttatt ccatcttttg ccatagctga ggcggaactt
     5401 tcaaaggaat aactctttac attatctaat tcagctattt tttccgcgtc cgttaaggaa
     5461 actggtgtaa gagaaaaact gcctggatct ggacgttctt cagtggtaga accgcttgaa
     5521 gaagtttgtt cctgtttttt aaatgaagct tctcgattgg cacttaaagt cactgttgct
     5581 ccaacttctt tttgtgcttg attcgctgct tcttctgcag cgcttcgaat cgtgaggcca
     5641 gctagcacaa aaataagaat ggctgtgaac acggcaaaca ataacaatga acgtccccat
     5701 tttacttttg tacttttcca tgcacgtttc ataaaattca tattttgcat ccctcctagt
     5761 agatgcattt gataatagac aacctatctt aaagctagct taaatcttat gctgtttgca
     5821 caccatcaac atgaggttct gtatatataa aaagtcacta tagttattgc aaaaaatgtg
     5881 gtaaaatgaa aaaagtatgt ttcaaaccga cgaagaagaa atgaaagagg aatagcaaaa
     5941 gaaatcctag atgctacaga gggattttta cgtgcatagt tgttctaaca attgctgata
     6001 ctagcgattt agtttatttg ttgtataata atggggaaca gctgaatact tatataagta
     6061 ttctggctat agcgtttcgc aaaagatcca ttttgttaat gaagtatatc caaaataaaa
     6121 tgcccatcca actgtcagaa cgcggatgtg ctggtaattt tcctgctgat tctccataag
     6181 ttgagaaata gtgattttgc atatgcttca ttggaatggc tcatatattt agataaagaa
     6241 gaaaatcgac atctttaaac ttggatgtca taagaagagg tgaagtttgt gagtaccatt
     6301 aaaattatcc cattaggcgg cgttcgtgaa aatggaaaaa acatgtatgt cgcagaagtg
     6361 ggcgaggata tttttatttt agattgtggg ctaaaatatc ctgaaaatga gttgttgggg
     6421 atcgacgtcg taatccctga ttttacgtat ttagaagaaa acattgatcg tgtagctggt
     6481 gtctttttaa cacatggaca tgcagatgcg atcggggcac ttccttactt gttgtcaaaa
     6541 atttctgtac cagtattcgg gacagagttg acgatcgagt tagcaaaatt gaatgtcagt
     6601 cgtaatgaag cagccaaagg atttaaggat ttccatgtgg tagatgagca tacagaaata
     6661 gactttggtg aaacagtagt gagctttttc cgtaccactc atacgatacc agattcagtg
     6721 gggatcaatc tgaaaacaaa agaaggtagt atcgtttata cgggagattt taaatttgac
     6781 caaacggcta tcccaatgta tcaaacagat tatgcacgat tggcggaaat cggcaaagaa
     6841 ggagtcttag ccttactaag tgaatcggca aatgcagaaa atcctcagcc tgtagcttct
     6901 gaagcccaaa tagcagatga agtatttgat acgatcaaat attgggaagg gcgtatcatc
     6961 gtcgcttgtg tagcaagtaa tttgcaacga gtgcaacaag tattggatgc agcagaaaaa
     7021 gcacaacgaa aagttgtctt gactggtcaa gatttcgaac gaattattcg cacagcgatg
     7081 cgtttagaaa aacttcaatt accaagtgaa gatctgcttg ttacgttgaa agacatgaaa
     7141 aactatgatc cagaagaact gatcatttta gaaactggcc gaatgggtga acccatcaag
     7201 tctctgcaaa aaatggcaaa cggtacacac cgtaatgtca agattgaaga aggagatttg
     7261 gtttatatca ctactacgcc aagcatcgcg atggaaacca tcgtggcgaa aacggaagat
     7321 atcatttatc gagctggtgg aacagtgaag ttgatctctg agaatatgcg cgtttctggc
     7381 catgccaatc ctaatgactt gcaattaatg atcaatttga tgaaaccaac atattttgtg
     7441 ccagttcaag gagagtatcg tgaattagct gctcatgcag atttggctca tgcagtaggg
     7501 atgccataca aaaatatcta tattactgga cgcggagacg tgctggaata taagaacaag
     7561 cgtatgagtg ttgcaggaac tacaagtgca gaaaatgtaa tgattgatgg attaggcata
     7621 ggtgatatcg gaaacattgt tttacgtgat cgaaaaatct tgtcagaaga cgggattttc
     7681 gtagcggttg tcactatcag tcggcgagaa aagaaaatca tttcaaaacc tcaaattact
     7741 tcacggggat tcgtttacgt aaaagctagc cgtgatctga tgaaagaaag cagcaatatg
     7801 attgaaacaa tcgttgagaa acatttagaa agcaatgatt ttgagtggag caaattaaag
     7861 caagacatcc gagatcaatt aggccgttac ttatttgaac aaacaaaacg ccgcccagtg
     7921 atcttgccag tcattatgga agctactcaa cgtcgaaata aaaaataaac aaaaagagta
     7981 agatgaacct ctctatagag tgctcgtctt gctctttttt tgcgaaaaac aaataatttt
     8041 tgaatacagc atccctcgct ttataataaa cgatagaata aagggagtga atcaatgatg
     8101 agtcaaacaa aagtcgaacg ttatttagaa gaacaaaaga tcagctatca accacttgat
     8161 tttagtcatg tggtagaaga atctttcaca gaagaactac aaaaaagagg gattgatcca
     8221 gctttgatat gtaaaactct cgtagtgaaa ggaaacaaaa ctggggtgat catcgcattg
     8281 attcctttga acgatcactt agattacaaa aaaacgagac aagtgacgaa agatcgtaaa
     8341 gttggttttc ctggaatgga ttttgtatta atgcatacag gatatcccca cggagcaaac
     8401 acccctattg gaataaaact cgcacatcct gattattgtt ttttagcaga cagttcttta
     8461 aaagaaaaag aagaagtaat tgtgtcaagc ggtgaaatcg ggagaagcct tcggataaaa
     8521 gtagccgatt tagaaaagat cgtccatcct atttacgctg atttaattag ttaagggacg
     8581 agaagagaag ctaagcagag cagagacctc aaacttgaga ttatatctgc ttggtacttg
     8641 gtgcatcctg tattatctgt tacaattgct ttgtacatac tgtactatca atccaatgaa
     8701 aatatattta gcagctatat tttttcattg atgtgcaagt gatgaaatcg tttttaataa
     8761 aaaagataca gagaaaacgc gaggtgtaaa atatgaaaat tacgtatcat ggacattcct
     8821 gtttaacaat tgaaatggaa aatggccaac gcgtgatgat tgacccgttt attacaggaa
     8881 atccattgac agacttaact gcagaagaag taaagacaga ttggttgctg attactcatg
     8941 gtcatagtga ccacatagga gatatgattc ctattgcaaa agaaaatcaa gccacaatta
     9001 tcagtattgt tgagattgct gattatgcga acagcagagg agtagattca ttcggtatga
     9061 atatcggtgg aaaacatgcg tttccattcg gcgtggtaaa attcgtacat gcacagcata
     9121 gttcaagcta tgaagtagac ggtatcgttc agtatatggg cgagcctagc ggaattatta
     9181 ttcaagcaga aggaaaaacg atttatcatg caggggatac tgcttatttc agtgatctag
     9241 gattgcttgc agaagaattc gatattgatg tcgctttctt gccgattggc gataactata
     9301 cgatggggcc agaagatgca gcaaaggctg cgcaaacaat tcgtgccaag aaagtagttc
     9361 caatccatta caatactttc cctgtcattg aacaagatcc cgaatctttt gtagagttac
     9421 tggaagatag tgaaggaaaa ataatgagcg tctcggaaac gctggaagtg taggcagaaa
     9481 atggcgacaa aacatgattt gattttagaa tatattgaaa gtttacctgt tggcaatcga
     9541 atttctgtaa gaagcatcgc aaaagaattg aatgtcagtg aaggaacagc gtaccgtgca
     9601 atcaaagatg cagaaaatgt cggactagtt tcaacaattc aacgagtagg gaccatccgt
     9661 atcgaacgca aattaaaaaa acatattgaa cgtttgacat ttggcgaggt cgtcaggatc
     9721 attgaagggg acgttttagg cggggcggca ggattagaca aggtcttgaa taaattcgtc
     9781 atcggcgcga tgactaaaca tgccatggaa cgctatatta cgccaggatc actaatgatc
     9841 gtcggcaacc gggcggaagt ccaaaaatta gcattggaag atggcgccgc agtattgatc
     9901 acaggaggat tcaatacgac tgctgagatt gcaaagctag ctgatgatct tgaaatgccg
     9961 atcttacgca cctcttatga tacatttaca gtcgcaacca tgatcaaccg agcgttaagc
    10021 gaccaattga tcaaaaaaga tatcatgctt gtcagtgata tctatatgcc agctgaaaag
    10081 actcattatt tgcagcagac agatacgatt tctgattatc agcgtttgtc agaagaaacg
    10141 cagcattcgc gttttccagt cgtcaaccgc catcatcgtt tgatggggat tgttacggca
    10201 aaagatgttt tagggaaatc gccaaaccag atcatcgatc gggtgatgac gaaagaacca
    10261 atcagtgtaa agaaaacaat gagtattgcc tctgttagcc atcaaatgat ctgggatggg
    10321 ctagaagtca tgccggtcgt ttccgatgac ttgacattgg aaggcttgat tacccggcaa
    10381 gatgtaatga aagcgatgca gcttgtccag cgacaaccgc agattgcaga tacgatttcg
    10441 gatcagatat ctggaaatat ccatacgatt gatactgacc gtgaaggaaa tcgtttggaa
    10501 aaccctaagt tcaaattcgt agtagcacca caaatggtca atggcgttgg aaccatttct
    10561 tttggcgtac ttagcgaaat catttctgat gtcgcacaaa aaacaatggt gatgaaccaa
    10621 aaacgaaata tcttgattga acaagtgaac ctacattatt tgcggctgat ccaattggaa
    10681 agcgaattag acattcgtcc acgtgtattg gaaatcggca gacgttccgc taaactggat
    10741 atcgaagtgt ttattgaaaa caccatcgtt tccaaagcaa tcgttgtttg tcaagtaatg
    10801 gaacgttctt agaaagcagg agtgaaatga cagtacagca agaaatagta gatacaataa
    10861 aaaaatacga taaaatcatt atccacaggc atttgcgccc tgatcctgat gcattaggat
    10921 cacaagtagg attagccgaa ctattacggg aaagtttccc agaaaaagaa atacgtcaag
    10981 taggtgaaac gattgaaggg cttcgctttt tagcagaaat gcaagaagtt gaagatgtct
    11041 tctaccaagg agcgctagtc atcgtgactg atacagctaa ttcacccaga atcagtgatc
    11101 aaagatatca gttggcagaa aaactgatca aaatcgacca tcatcccaat gatgaccctt
    11161 atggagatct tgtgtgggta aatacaaaag caagtagctg tagtgagatg attgcagaat
    11221 ttgctttgat gtttcctgaa tctttaaaga tgaacgttaa ggctgcacgg ttactttatg
    11281 caggaatcgt cggagataca gggcgctttt tgtatccctc cacgacgtcc cgtacattag
    11341 agattgcttc gatattgaga aattatccat ttgatgcaag tgctttgaac agagagattg
    11401 aacaaatgcc gatgaaagtc gctaaacttt ccggttatct ttatcaaaat atccaggtgg
    11461 atgaaaatgg tgcaggaaga ataatccttc cgcaagaaat tcttgataaa tatggaatcg
    11521 atgattcaga gacagcttcg atcgtttcac ttcctggagt gattgacgaa gtgctggctt
    11581 gggggatttt tgttcagcag ccttcagggt attaccgcgt acgtttacgt tctaaggggc
    11641 cgatcatcaa tgaattggct aaacgccatc atggaggagg ccatccatta gcaagtggtg
    11701 cgaatgcaaa agatttagca gaagtagagg aaatctataa agagatccaa gatatatgcc
    11761 aatcgtataa gagcagaaaa acagaatgat acttcgttcg aaaacgtgcc ttttgtagta
    11821 aaaatcacaa atgtatcttt tcaagatctt ctttttccgt tacaatagtg ctcactaaag
    11881 attttaaagg agaaagaaga tgacattacc aaattgccca gaatgtggct cagagtacac
    11941 atatgaagat cgaggcttgt tgatttgccc agaatgcggg aacgaatgga acaaagaaga
    12001 acaagaaact gtcatagaag acggattagt ggtattagat gcaaatggaa accgtctgtt
    12061 agagggcgat agcgtgacag tgattaaaga cttgaaagta aaaggtgcgt caggtgccat
    12121 taaacaagga acaaaagtca agaacatccg tttagtagaa ggcgatcata atatcgattg
    12181 caaggtagaa ggatttggac cgatgaaact gaaatcggaa tttgtgaaaa agaattaaag
    12241 aatattcaag gtcttttcac attcttttcc tactactaat gataaaataa agtaaacgaa
    12301 aaggagtgaa acgaaaatgt atggttatgg aatgtatggt tttttagatc cgacatttct
    12361 tttggtcatt cttggattag ttatttccgg aattgcgtca gcgtacgtca atagtacttt
    12421 ccgaaaatac gatcaagtga gaagcaaaaa caatgtgact ggtacacaag cagcacaata
    12481 tatattacaa agtcaacaga taaataatgt cggtgttcaa caaattgccg gagatctcac
    12541 agataactac aattcaggta ataagatgtt gagtttgtct caagcaactg cacagtctac
    12601 ttcagttgct gcgataggtg tagcagctca tgaatgcgga catgcggtac aagatgcttc
    12661 gggttatgtc ccattgaaga ttcgtgcagc acttgttcca gcagctaact ttggttcgat
    12721 gatttcgttc cccttgatta tggtaggtgt attgcttagc tggaactcta cgttaatcaa
    12781 tatcgggatt ttcgcttttt ctttagcatt acttttccaa ttggtgacat taccagtcga
    12841 atttaacgcg tctcggcgag caatccaaat tttgagtgaa ggcggactac tgacggaaga
    12901 agaagtaccg atggcaagac acgtattatt tgcagcagca ctgacttatg ttgcagcagc
    12961 gctttcgaca ttcctgcagc tgttgcgctt gatcttattg ttcggcggca accgaagaga
    13021 ttaaaataaa gaagttgtga aatcagcgct ttgatctgag ctaataccga gagttagctg
    13081 attgaatacc atttatttgg aata
//
LOCUS       DAZUKC010000069        13012 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000069 DAZUKC010000000
VERSION     DAZUKC010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.070"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(65..1543)
                     /locus_tag="ACG80T_002018"
     CDS             complement(65..1543)
                     /locus_tag="ACG80T_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4841652.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            complement(1841..2776)
                     /gene="murB"
                     /locus_tag="ACG80T_002019"
     CDS             complement(1841..2776)
                     /gene="murB"
                     /locus_tag="ACG80T_002019"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFS4841653.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(2849..3769)
                     /locus_tag="ACG80T_002020"
     CDS             complement(2849..3769)
                     /locus_tag="ACG80T_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4841654.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            3882..4634
                     /locus_tag="ACG80T_002021"
     CDS             3882..4634
                     /locus_tag="ACG80T_002021"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFS4841655.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            5498..6031
                     /locus_tag="ACG80T_002022"
     CDS             5498..6031
                     /locus_tag="ACG80T_002022"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFS4841656.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(6521..7045)
                     /locus_tag="ACG80T_002023"
     CDS             complement(6521..7045)
                     /locus_tag="ACG80T_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFS4841657.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(7038..7511)
                     /gene="tsaE"
                     /locus_tag="ACG80T_002024"
     CDS             complement(7038..7511)
                     /gene="tsaE"
                     /locus_tag="ACG80T_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFS4841658.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(7634..8614)
                     /gene="pta"
                     /locus_tag="ACG80T_002025"
     CDS             complement(7634..8614)
                     /gene="pta"
                     /locus_tag="ACG80T_002025"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFS4841659.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(8767..9447)
                     /locus_tag="ACG80T_002026"
     CDS             complement(8767..9447)
                     /locus_tag="ACG80T_002026"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFS4841660.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            9573..10442
                     /locus_tag="ACG80T_002027"
     CDS             9573..10442
                     /locus_tag="ACG80T_002027"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4841661.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            10468..10983
                     /locus_tag="ACG80T_002028"
     CDS             10468..10983
                     /locus_tag="ACG80T_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFS4841662.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(11030..11593)
                     /locus_tag="ACG80T_002029"
     CDS             complement(11030..11593)
                     /locus_tag="ACG80T_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4841663.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(11648..11827)
                     /locus_tag="ACG80T_002030"
     CDS             complement(11648..11827)
                     /locus_tag="ACG80T_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841664.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(11904..12413)
                     /locus_tag="ACG80T_002031"
     CDS             complement(11904..12413)
                     /locus_tag="ACG80T_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841665.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(12509..12889)
                     /locus_tag="ACG80T_002032"
     CDS             complement(12509..12889)
                     /locus_tag="ACG80T_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4841666.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
BASE COUNT         3791 a         2612 c         2242 g         4367 t
ORIGIN      
        1 ttttctttga aaaaaacata aatcagctaa taaatatgtg agacaaagct gccatccata
       61 aatactagct gattttttca gttaaatgtc tatttttttc tctgatgatg gtatttccaa
      121 aatattccca gtcatctgga gtcggtggat ttttccaaat tatctgttct ttattaaatt
      181 tttcaagtaa acaatcagat aaataaatat tcgtacgaga agtgattcga ctctcaatca
      241 caatattgta atttttaaat cctttcactt gagaaataat gtaatcggta taagcagatc
      301 cttgcgaaaa atcaatacag acatgaatcg gtgcttcaaa atatgatacc ggacaaatac
      361 cgaccaataa aaagagataa tcataaaata agcgaatcat gaaacgttca tcttttccca
      421 agaaatagcg tttattctct tgtatgacct tttggattgc tgtactgaat attggatacg
      481 tctcgaagaa aaactgtagc tgctttttag acgtaaagct agtcatctca aagtcgaata
      541 tccaattttt tcgattgaca tgcatcatac ccatacgaag ttcttttcgt atatatagtc
      601 tttcttcttc agagatttca atcgaaaatg gtaattgatc aatcaattcc actgacattt
      661 tttgactaat catgtggata tcatgaaaat gagcttccgc aagtccaaat gcgccatcta
      721 attcaaattc ttcttccgtg atcaaaaaca acatgatata agaaatctca tcaatgatct
      781 gctctttagg gatatgaaga aaagaaatat gtgataaatc cttgatgatc ttcattcctt
      841 ctgagctctt aaagtattca tctgaaaaga aacgttttcc gattggttga ttcccatagg
      901 ccctcaacaa actaatagtg gttagcaaat tgaatttgat tcgttttgtt gcagacagtt
      961 ttaaattgaa cagatattca aaatattgca cgatactctt tgcttcattc atgacgcttt
     1021 ctctaaatgg catagaaaaa ccattaaaga ccccgaaata taccccaaat aatacgttcc
     1081 gtatgtttat ttcttcccca actaatatgt ttttctttaa ctttatttga tgctgttcaa
     1141 gaaaagagac taattctctt ctttttacat aggctcttga tatgcttaaa aaatgattct
     1201 ctgcaaattt ttcgatcgtt acttttgaat ccacgatctc cgttaacagt cgagcaattt
     1261 ttgatcgttg aaagtagatt cttcttaaat ttttgatatc cgtcaaattc agacgttcga
     1321 catggatctc tgcttcttct gaaatttgga tctctggctt tgttttgaat tcagtaaatt
     1381 ctttatttaa ttctatgata taatttcttg ttttaaattt agacaaacct aatgtttcat
     1441 ataacttagg cagcactaca taattctttt tctcaaattc taaatatcta gctagcagca
     1501 gtttttctct atcttcctct tctataaggt aatccaactt catatcctac acctcaaaaa
     1561 atgcctcata ccaattactt gatatgcaga tgaaagttca tcttttcacc tatactgatt
     1621 ttatgatgga ttatgcttgt tttttcatca gttattctca ttttttatct tttttttgag
     1681 aatattgcaa aaaatggaaa aatttatgag gaaatattct atattttttg ttttattgtg
     1741 ttaaaatgta caagatttgc gccatttgcg taaacaacca caaaaaagag caggacggcc
     1801 accgtcctgc tcttttactt attttaagtt ccattactta ttaattggct aaacttctgc
     1861 ttgtttctga tattgcttgt ttttctccaa tgatccgcac ttctgtttct aaagacacat
     1921 caaatttttc tttgattact tcttggatgt gcgcaatcaa ttcaatataa tctgtcgctg
     1981 tcgcatgatc gatattgacg atgaatccag catgtttttc agagacttgt gcaccgcccc
     2041 atttcaggcc ttgcaatcct gcttgttgga tcagctgacc tgtatagtgt cctactgggc
     2101 gcttaaagac acttccgcat gaagggtatt ctaatggctg ttttgattga cgcaattcgg
     2161 tcaactcatc cattcttgct ttgattgctt cataatcccc tgtttgcaaa ctgaatcgag
     2221 cttccaatac gatagcacgt aattcttgga ctttactgtg acgataagaa aataccattt
     2281 cgtcttttgt caaagttttc aatgttccat cttcaagcaa aagatccact tcagcaaaaa
     2341 catctttgat ctctccgcca tacgcacctg cattcatata aaccgctcca ccgacacttc
     2401 ccgggatgcc gcaagcaaat tcaaatccag tcaatgattc tgccaaagct gcataagtcg
     2461 tatcaatcag tgaggcacct gcttccgcaa tgaccatcgt tcctttgaca tgaatctgtt
     2521 tcatttcagt cagcatgatg actacaccgc gaatcccgcc atcttgaacg atcagattgc
     2581 ttgcatttcc taataccatc caaggtatat catggattcg gcaataatcg atgatttgct
     2641 ttacttcttc ttgttttttc gggaatgcaa ggacatcagc tggtccacct gttttcgtaa
     2701 acgtatagtt catcaatggt tcatcaaaca atatagtaat cgtggctaag ctttcaacta
     2761 tttcttcttt attcactgag gattttcctt tcttttctca aacgtcctca ttattctatc
     2821 acgcttaata caatcttgcc agtcttgttc aaagttgatg acggtagtga tctttaagca
     2881 cttcctcaca taattcatga cttcttacaa cttctttttg tttaagcaat tctgtttccg
     2941 atttacctaa acggatctga gaaaagaagg cctctaccat tggctcaaac ccgcgtttga
     3001 ctagcgtgtt tgtccagtcg ccaaattctt tcactttttt tccatcagca tcttgaatga
     3061 caagctgggt cagatcgttt acttcataag taccattttt acttgttact tgatattttt
     3121 ctatattagc gccacttgct aaatccatcg ttaaaatagc cgtttgattt tgtgtttcta
     3181 attgtaaaat ggcatattcc aaaaattcct ttgtttctct tatctggtat tttgttctca
     3241 aaatgggttc atctaataaa tacacagctg tatcaataag atgtaaaaac aaatcataaa
     3301 ggacgaactc tgtcgtttct tttgccgcta ttcgattttt ttgcaggtgg atcaaccgct
     3361 tatctgggat acgtttcaat tcttctacca taggagcaaa gcgccggttg aaaccgatca
     3421 tcaaaattat ttggttctta tcagctaatt cttgcaattc acgaacttca tttagatgga
     3481 cactcagtgg tttgtcaata taaacatgga taccattttc tagacatttt ttggctaatt
     3541 cataatgtac ttttgttgct gcgtggatca tacatgcttc gattttttct tctaaaagct
     3601 catcgatcgt ttcatataga tgttggaacc cgtactcttt ttgaattctt tcttttgttt
     3661 ctttatttct tgttgcaaaa taaaaatcaa gtgttccttg ttttttcgca taagttggta
     3721 aatatgcttt ttctgcgata ttccctacac cgattacacc aatcttcatc tactttcctc
     3781 ctggatcatt tagttacttt catcttacac cattttttac taaaatgagg tacaatagta
     3841 tcgcaaaggt aaggaaatta attcgaagga gtgtgctttt attgaaattt atctcttgga
     3901 acgtaaacgg gcttcgtgcc atcgtaaata aaaatttttt agaagttttt catgagttag
     3961 acgccgattt tttctgtctg caggaaacga aactccaagc aggacaaatt gatttagatc
     4021 ttccaggtta ttatcaatat tggaattacg cggaacgaaa aggctattcc ggcacagcga
     4081 tttttgctaa aaaaccagca ttgaatgcca cttacggaat gggtatcgat atccatgata
     4141 cagaaggccg tctgattact ttggaatatt ccgatttctt tcttgtaacc tgttacacgc
     4201 caaattctca aagcgaatta aaacgtttag attaccgatt agaatgggaa gaagcctttt
     4261 ataattattt ggaaaatcta aaaaaacaaa aaccagtcat cgtgtgtggg gatctaaatg
     4321 tcgctcatca aaagattgat ttgaaaaact ggaaaacgaa tcaaaaaaat gctggattca
     4381 ctccagaaga aagagcggct ctttctcgac tgttggataa cgggtttatc gatacattcc
     4441 gttattttta tccaacccag gaaggtgtct actcttggtg gaactaccga ttcaattcaa
     4501 ggaaaaacaa tgctggttgg cggatcgatt actttttgac gagcaaagat ttagagccag
     4561 gactagctga tgcaaagatc catacggaca ttatgggcag tgatcattgt ccagtagaat
     4621 tagatttgaa ataaagaggt tgtgaaaaag tcgtttagtt tcgagtatta agaaaaattt
     4681 tgtgaaaatg gctttccaca ttttgcataa atttggcata ataccgagaa actggctttt
     4741 gaacactgtt tatcgagaga ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc
     4801 aaacaaaaat agttcctcat atttttcgtt tggttgggct tattaccgca gatacaagct
     4861 tttgaacacc gtttattaag aggttgtgaa aaagctgcac tgcatcaagt aataagaaca
     4921 gccaaacaaa aatagttcct catatttttc gtttggttgg gcttattacc gcagatgcaa
     4981 gcttttgaac accgtttatt aagaggttgt gaaaaagctg cactgcatca agtaataaga
     5041 acagccaaac aaaaatagtt cctcatattt ttcgtttggt tggacttatt accgcagatg
     5101 caagcttttg aacaccgttt attgagaagt tgtgaaatca gcgtcctgac ctgagtatta
     5161 gagaaacaaa tggcaaaata gttcctcata ttttgctatt ttttgagcta ataccgaggg
     5221 tcagctgatt gaacaccgtt tagtggagaa gttgtgaaaa agtcgcctac tttcgagtcg
     5281 taagaaattt ttttgaaaat ggttcctcac aactttgcat aaatttgact taataccgag
     5341 aagtcatgat ccccatctaa ctccaaatca caaggaatga gttcaaaaac tagcttttct
     5401 caacaaatat tacataaaaa attctatgtt ttttgttaaa tttatttgct tttttcaaaa
     5461 gtatgttaca gtaatttccc aaggaggttt tacagaaatg aattttgttg ccatggattt
     5521 tgaaactgcc aatcatcagc cttatagtgc ttgttctctt gcgctagtca tggtaaaaaa
     5581 tagccagatc gttgatgaat tctatacatt gatacagcct gagactcctt ttttctggcg
     5641 aaatgtccaa attcacggaa tccatcaaga agacgtacgc aatgcgccga aattcccaga
     5701 agtctggcaa actatccaac catattttca aaaaaatcgt ttagtcgttg cacataatgc
     5761 tgcttttgat acaaaggtct tagcgggatg cttagattat tatcatcttg aacagcctaa
     5821 ttatctttct ttatgtacag tgaaaagtag ccgtcgcctg tttcctgaat ttccaaatca
     5881 tcgattgaac actgtgtgcg aaaatttgaa catccaatta gatcatcacc atgatgcatt
     5941 ggaagatagt cgtgcatgtg cggaaatcct gctttatcaa gaaaaaaaat tcgggacaga
     6001 tccattgaaa aaacttgtga ctataaagta aaagaatact tatgaaacac acaaagaggt
     6061 cgtgagcctg ccgtttcact ccgagtcaca aggagcaatt tcaaaaaata gcttctcatg
     6121 tttattaaat attgtgactg gtgaccgagg agctggcagg agaacaccgt ttattggaga
     6181 ggttgtgaaa aagctgttct gcatcaagta ataagaacag ccaaacaaaa atagcttctc
     6241 atatttttca tttggttgga cttattaccg cagatgcaag cttttggaca ccgtttacca
     6301 agaggttgtg aaatcagcgt tttgacctga gtattagaga aacaaacggc aaaatagttc
     6361 ctcatatttt gccgtttttt gagctaatac cgaaggtcag ctgattgaac accgtttatt
     6421 caaaaaaaat ggactgcgac acgacttttg tcacagtccc ttcgtgtgtt tttctgcctt
     6481 ctcaaaagga tgacagtttc tcactctttt cctatttctt ctattcaatc aaataactca
     6541 ttttatatac atctaaaaat tcacctagat ctgttcttgc ccctctaggc atgaccgctt
     6601 cgatttggaa gcccattttt cggtaaaggt gtacggctct ttcattacga acttgaacgt
     6661 caagctccaa tcgaaaaagt acacccattt ccttcgccca actgatgatt tcttctagca
     6721 taagagtgcc tagtcccatt ccccaatatt cctgcagaat actgataccg acttctccca
     6781 cgtgagacaa acgaaattga ctgtctgcct tgacagatgc tgttccaata atcgtccctt
     6841 catagattgc aagcagcaat aaattattat tgctttcgta tatataatcc aactcttctt
     6901 ctaaggtttc tggtggtaac aggagttctg cttcatccaa aactaaaaat tctgtctcct
     6961 tattaacttg tcccatcatc aaaagaatgt cttttgcatc tcctggcact gcttcccgca
     7021 agatgatccc aacttcttca ctcatgattt tcagcccatt tttctagaat tcgctgtttg
     7081 aaggtttcac tttgctcccc gtatgagatt agtttcacat accgcttttc taaatctgta
     7141 tcactttttt ctaaaatcag ttccaaataa tcctccggca gtgcttcttc taatagattt
     7201 ccccattcaa caacggaaag cccatcacct tcaaaatatt catctaaccc taaatctgct
     7261 ccgctagctg ctacccgata aatatccata tgatacaacg gaatccgacc ttgactatat
     7321 tctctaataa tcgtgtatgt cggacttttg atcatttgcg ttatccctaa tccttgggca
     7381 attcctttcg tcaacgttgt tttacccgca cctagatcac cagtcaatat cagattgtca
     7441 cctggcattg cggaatgacc aattgcctct gcgaattgtt ctgtcattgt taatcctgat
     7501 agttcgatca tttttcatct tctcccgctc ttctttcttt ttgccagtat atcgcgtaat
     7561 gaagtttttt taaagaaaaa atgaactagt caagaacagc agctgctgtt tttggctagt
     7621 tcgcgtgtat ttgttaatca atcaatgttt gtgctgctgt gataatcgat aatttatata
     7681 catcttcttc atttgctcca cgagacaaat cagaaaccgg tttgttcaag ccttgtagaa
     7741 ttggtccgat tgcttcaaag ttaccgaaac gctgagcaat tttatagccg atatttcctg
     7801 actgcaattc tggaaataca aagactgttg cttgaccagc tacttctgaa tctggcgctt
     7861 tcagttgtgc aactgaagga acataagaag catcaaattg caattcgccg tcgatcatgt
     7921 attgtggcgc tagtttttta gcgattttag ttgcttcaac tactttgtct acttctggcg
     7981 atttcgctga gccttttgtc gagaagctca tcattgctac ttttggttcg atatcgaaca
     8041 attctgctgt ttttgcagaa tctacggcga tttcagccaa ttcttgcgca gttggattca
     8101 cgttgatcgc acaatccgaa aagacatatt tttcttgatc tcttccacgt accatgatca
     8161 tcgctccgct tgtccgactg atgccaggct ttgttttgat gatctgaagt gctgggcgaa
     8221 cagtgtcgcc agtcgaatgg atcgctccag agaccattcc gtctgcaatc cccatatacg
     8281 taagcatcgt accaaagtaa ttcacatctt ttaaaatctt ttctgcttgt tctttggatg
     8341 cctttccatt tctacgttca acaaaagtat ccaccatttc atcccactta tcgtaattat
     8401 cagggctgat gatcgtaaag ctagatgttt tgattcctct ggcatgcgct gcatttctta
     8461 tttcttcttc gttccctact aaaatgggtt ccacaagttc ttctgctttc aaccgggcag
     8521 ctgcgcctaa aatacgagca tcagtagctt ccgggaaaac gattttgatc ccacggcgaa
     8581 caattttaaa tcttaaacta tcaaacaatt ccacaagatg acctccagac gtttttatat
     8641 gtattatcat acactattcc agaaaaaaag aacagtttat tttatctgta tcagtcaaaa
     8701 atgctggaaa aattaagttt ttcacataca aagcgaactg cactataaca ggaaagcgct
     8761 gttttattac actgtttcag gcagttgcca atcaatcggt tcttctccta acgcaaggag
     8821 cgcatcattc gttttcgaga agggtttgga accaaaaaag ccacgacttg cagataacgg
     8881 actgggatgt ggagatgtta tgatgatatg gcgactctta tcgatcattt tgattttttc
     8941 ttgtgccggt tttccccaca agataaacac gattggtttt tcccgttcat ttaatttttc
     9001 aatgatcgtg tctgttaatc tttcccaacc tttcccgcga tgagaatacg cttgcccttc
     9061 ccgaacggta agaacggtgt tgagtaaaag gactccctgt ttagcccatg acactagatt
     9121 tccatgttga acaggtgcga ttccaagatc atcataaagc tctttataga tatttcttaa
     9181 cgatggcggg atctttacgc caggttgcac agaaaaagat aagccatgag cttgattttc
     9241 tccatgataa ggatcttgtc ctaaaatcac cactttaact tcttcataag gagtcaattc
     9301 caatgcttca tagatatgat acatatctgg atggatcttt tgggacgcat attctttttt
     9361 taaaaattct cttagatgca gataataatc ttttgaaaat tctgaggaca aaacttcttg
     9421 ccaactgtta tgaatgattt ctttcaaaaa ctccacctcc taataaaaag aataactcac
     9481 attttcttca atcacaaggg acaatcttca ccctcagcac ttgattaata tggtaaacta
     9541 ggaacaagaa gcagaaagga acgataatca ttatgatcaa attaattgca tcagatatgg
     9601 acggcacatt attagatgcc catatgagtg tttctcctga aaatgccgag gcgatacgtt
     9661 ttgcaaatga tgccggtgtt gagttcatgg tcgctactgg tcgaaactat caagaagctc
     9721 gcgcagcttt agatgaggta ggaatcgatt gtgcgatgat cactttgaac ggggcacagg
     9781 tatttgataa agaaggaaat tcattgttta ccgtgtcgat tccaaataat caagcaatct
     9841 ctgttttaga tattctagat gctaacggta tttactatga agtagcaacc aacgatggtc
     9901 tttattctga aaatcaagcg aaacgaattg aaagttttgc ttccatggtg gctacccatt
     9961 tacctcacct tacgtataaa atggcgattg ccatggcctc agctaagctg gagttattgc
    10021 acatcacata cgtggacagt attcgtgcta ttttggaaga aaagaatctg gaagttttaa
    10081 aaattatttg ttttcataca gaagggccag ctgtattagg tccagttgga aagcagattt
    10141 ctcttttagg cgatctagcc gttacttcct ctggtcaaaa caatatcgaa gtgaaccata
    10201 aaaacgctca aaaaggaatt gctgttgccc atgtcgctca cgagcgtggg atttctttag
    10261 aagaagtcat gacgattggc gataacttca atgatgtcag catgctgcaa gtagcaggag
    10321 tcagtttcgc aatgggaaat gcagaaatcg aagtaaaaga ttatgccaaa tatacaacgg
    10381 acacaaacct tgaatctggc gtaggaaaag ccatattgcg cgcgattgca gaagacttgt
    10441 agttttatct agaaaagagg gatatagatg tcagaatttt tcattcagga aaagcagctt
    10501 ggacgtgcaa ctcggacaat tgtcaaaaac gaaaaaggac attccctgtt tttgatggtt
    10561 ggtcgctggg gaacaaaggg cgatgcattg tctctttatg ccatgaatgg cgatctcgtt
    10621 gctcacatca agcagataag cctgacattc ggtacgcgtt ttgagctgta taaagagttc
    10681 gaaaaggtgg gaaccatgca gaaaatattc aattggcccg gtgactttta ttatattcgt
    10741 agacttcact ggacagtcca aggtgatatc tataatcatc aatatcaaat ccatcatttc
    10801 aatcagcaaa tcatgaaaat ggataaagca acactttttt caggagatta ctatgtattg
    10861 gatgtctcaa atgatgaaga tgctccagtt tgtatttgta ttgctgcggt tttagattac
    10921 tggctctacc gtaaagacaa aagtcgcgat aagcctgttt cttaccgact taacttaagt
    10981 taaaaagatg tacctgaaat gcggaaatac gcatttcagg tacatctttt tagatattct
    11041 tttgcatatg atcataaaga tgaccactga tcatccgctg accgacaact ttgaagtcat
    11101 ggcgttcgta taatttttta gcctttggat ttttttcatc cacacttaga ccgatgacgg
    11161 agcgattagc tttcttcgcc aatttaggta atgcttctaa taatcgcgaa ccgatgcctt
    11221 gtcctctaaa atcttctgat acactgatag agtccaaata ccattcgttg ggaaacgttt
    11281 ctttatcaat gaacaaacgt gtagacgcat ctagctgata tttctctaag ataggtacca
    11341 gtggctgatc gatgattgat tcttcttcat ctgtataacc gtaagcaact cctgcaactt
    11401 taccgtctat ttcttctacg atacctcgtg aataactgta acgataattt ggatcagtga
    11461 ttgcttcttc taaaatctct aaagtctttt gttcccctgt ctgcttcaaa aatggcagtt
    11521 ccatatcttt tagaatcact aagatcaatg gggcgatttc tggagcatct tcttttctcg
    11581 caaaacgaat catagtttcc tctccttttc tccattctaa catgcagaaa aagaaatcgc
    11641 aaaatcgtta ttttaaaaaa tcttcaatct tttcagtaat gaaataacca tgagtaacga
    11701 ttcttgccgc ttcagttcgg caataagggc agacgactgg catttcttta tcattatgtt
    11761 cgactacatt attgacttct tcgtatgcaa atttttttcc gcaattaggg catttcattt
    11821 tttccatgtt atctcaactt cctttcatct ttactctctt agcttactct tatattgtaa
    11881 aaaagagaag caatatcctt cttctatgca gatatccata cctgacggtc ctgttctcca
    11941 tctattaatc ggactttata ataaggacgt tcatctttga ctagaagtgt ccactctttc
    12001 acttttgctt ttttcccgta gtagctgact gcttccttca tcgctgcttg cggttgtttg
    12061 aactcttccg ttttttctat atcaaaaaga tgttctgcgg ccttttccgt gtcatttcgt
    12121 acggttgttt tcccagtgga tggattgact gctatgactt gtccttcaca aaaaaactgg
    12181 tattcatatg ttgcattcgt aggattctcc aaatcttttt caactgcaaa atgaatagca
    12241 gatatgttat gttcttttgt ttcgtttttt acgattgctg ctgctttttt caatgagatc
    12301 cgatattcct ggtcactaat atcatccatg tgaaaagcgg cattgagcag tcccgtcata
    12361 ccggtaatcc ctattacgat gagggcagca acaccaaaca tcattttttt cattcttcat
    12421 tctcctgttt ctttttttta aagaatatca agaaatgaac gaatgaaaaa tcgaatgaaa
    12481 cgaaaaaaaa gtgacaattt tgttatcttt attcgtgcag ttctaatggc aatccgtctg
    12541 gatcaaagaa aaacgtcatt ttttttcctg taaaggtatc ttctctaatc ggttctgatt
    12601 cgatccctaa ggactgcaat tctgcaattg tttcttcaat atcgtctact ttgaaagcaa
    12661 gatgacgcaa gccacaagct tctggatagt ttggtcgttg tggcgcatta gcaattccaa
    12721 aaatctccaa ttcagcactt cctagtttca gatctaattt atagtcatct cgttctttgc
    12781 ggtaattttc tctgattact tcaaatccta atttattaac ataaaaatca cgtgatttct
    12841 gataatctga aacaatgatg gctatatgat ggatcgttgt aaggttcata tgagcactcc
    12901 tattcttcgt ttaagctcgg atctatatta atctccagtt ccattgtaaa tgtgtcagat
    12961 tgaaaaaaat agctgttttt cctttttata agaaaaaagg caaaaaaaac ag
//
LOCUS       DAZUKC010000070        12549 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000070 DAZUKC010000000
VERSION     DAZUKC010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.072"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            109..1992
                     /locus_tag="ACG80T_002033"
     CDS             109..1992
                     /locus_tag="ACG80T_002033"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFS4841667.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(2067..2351)
                     /locus_tag="ACG80T_002034"
     CDS             complement(2067..2351)
                     /locus_tag="ACG80T_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFS4841668.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(2341..3075)
                     /locus_tag="ACG80T_002035"
     CDS             complement(2341..3075)
                     /locus_tag="ACG80T_002035"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFS4841669.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            3204..3827
                     /locus_tag="ACG80T_002036"
     CDS             3204..3827
                     /locus_tag="ACG80T_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFS4841670.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            4019..5140
                     /locus_tag="ACG80T_002037"
     CDS             4019..5140
                     /locus_tag="ACG80T_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFS4841671.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            5147..8275
                     /locus_tag="ACG80T_002038"
     CDS             5147..8275
                     /locus_tag="ACG80T_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4841672.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLNLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            8294..11488
                     /locus_tag="ACG80T_002039"
     CDS             8294..11488
                     /locus_tag="ACG80T_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFS4841673.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFHQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(11634..12341)
                     /locus_tag="ACG80T_002040"
     CDS             complement(11634..12341)
                     /locus_tag="ACG80T_002040"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4841674.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         4281 a         2212 c         2646 g         3410 t
ORIGIN      
        1 gggatgatag aaaaaaatag tagagaaagg acacgattct acttgctatt ttcacttaat
       61 acccgtatat tacgaatgaa aaaagtttat gaaaagttgg aatattgaat ggaaaaacaa
      121 aaaaggttaa aaaacagccg ctacatcact gggtttgatg ggctccgttc attagctgtg
      181 gtgggtgtta tcttctatca ccttctgcca actagtatga aaggcggcta tttgggcgtt
      241 ccgatttttt tcgtcgtatc tggttatttg atcacggatc tgctgcggca ggaatgggaa
      301 caaaatggaa aaatcaatat ttggcaattt tatgtcagaa gaatgaagag actctatccg
      361 gcacttgttt ttatgcttgt gacagcttcg gcttatatca ctttgtttca gcgagggctg
      421 ttaaacaatc ttcgaggagt tgtggtcagc agcttactgt atgtcaacaa ctggtggcaa
      481 atcaataacg gtttatctta tttcgatcga tttgctaatg agtcaccgtt tacgcatatc
      541 tggtctttag cagtagaagg tcaaaactat ttggtttggc cgatcttgtt tgtattgttg
      601 atggtatttg ttcgtaaaag aaaatggatc ttttatactg tgttaggtac atccttcctc
      661 tccgccatct tgatgatgat cttgtataca ccaggaggcg atccaacccg tgtttattac
      721 ggtaccgaca ctcgtctgtt ttctatatgg ttgggaagtg cgcttgcgtt tgtttggcca
      781 agtacgcgtt taaagaaaaa catcccgaaa caagcgaaaa atattctgaa tatcgctgga
      841 ggaatctcgc ttttggcact gattctttgc ttcttctttt tagatgatca ttacaatttc
      901 atctattacg gaggaatgct cctagtgagt ctgttatgcg tagtgctagt tgcagtgaca
      961 gcacaccccg gagcaagttt gaatcgatgg ctgacaaatc cagtatttac ttggattggc
     1021 aaacgaagct atggcattta tctgtatcag tttccagtta tgatttttta tgaagcaaag
     1081 gtgactgata ttgccgatca tgttttgttg catactttag tggaaatcac attgattcta
     1141 atcatcagtg aactttctta tcgatatgtg gagcgtccgc tagcacgttt tgattatggt
     1201 cgtacattcc aagtgatgaa aggatggttc agcaaaccga tcatcagtaa gagcaaacca
     1261 tgggttgttc caggcacatt acttgtagca attgcactcg ttggtttcgt tacggcaccg
     1321 aaaaacgctg taacagccga tcaaaaacaa ctgcaggcaa aaatcgctga aagtaaaaaa
     1381 ttagcagaag aaaccagaaa caattcagaa aacaccacta gttcggttga ccaagcgatc
     1441 cttgataagt acgagttgtc caaggcacaa gggaataagg cacaaaaatt agaactaacg
     1501 gcatttggtg attcagttat gttggatgct gcaacggatc tacaagaact ttatccaaaa
     1561 gtagtggtag atggagatgt cggacgacaa ttatatgcca gtgtaccgta cattgaaaaa
     1621 ttaaaggaac aagatttgtt gaaagacact gttctgattg gactaggaac gaatggctca
     1681 ttctcggaag ctcagtttga tagtgtgatg gatgcaatcg gtgatcgaaa agtatactgg
     1741 gtcaatgtcc gagtaccgac gaagcgctgg caaaatgacg tgaatgcgat gcttgaacag
     1801 atgacgaaaa aatacgacaa tatgacgctg attgattggt acagctacag taatgaccat
     1861 agtgactggt tttatgatga tcaagtccat ccaaatcctg aagggatgac acattacatt
     1921 cacttagtca gcgaagcctt gctaggagga agttcaagta cgaacagcag tgagacaaac
     1981 gtgacagaat gatagctaca aaaaacttca ggcaaaaaca tttgtttttg cctgaagttt
     2041 ttttatattt accgtgtttt taacattcat atagattcag gaagcaaaac ggttgagtgt
     2101 gtctgtaaat aacgttcttt ttgttttcgg gtgagttttt tgaatgcagc ttctgctttt
     2161 gttgcttcgc ttcttgaaga aaatacttct gcatggatca tcttaacagg tcttcttttt
     2221 tgaagacgag tatatttagc accagtacca ttgttatgtt cctctaatcg tctagaaaga
     2281 tcggttgtat atccaccata aaatgtctga tctttacaga ataatacgta aaaataatgt
     2341 tcatttgcca tagagcatct cacgaatctc tgcagtgtat tctccatcca tattgtaagt
     2401 gatcaatggt ggagcgactt tgaatccgtc taatttgcct tctttgattc cttcaactaa
     2461 caaaatattt gcctcttttc caggttttgg gtaaacaaat tggatccttt taggtgcaat
     2521 tcgatagtct tccatgacct gaatgatttc taagaaccga tcaggacgat ggaccatagt
     2581 aaagcgtcca tttgttttta gtagcttact tgaaatatcg atcacttctt ctaacgtcgt
     2641 atgaatctca tgacgggcga ttgccaggtg tttattaggg ttcgttttat ttgttggcag
     2701 ccctttgaaa taaggtggat tacagacgac taagtcacaa gaattatgtc gaacgacaga
     2761 taagctgtct ttcagatcga tggtgtggac agtcatttgt tcatttaaat ggttaagcgc
     2821 gatactacgt cttgccatgt ctgctagacg aggttgaagt tcgatagcgt cgatagccgc
     2881 ctgggttcgt ttacttaaaa aaagtccaac tgcgccattt cctgcacaaa ggtcaacaat
     2941 cttccctttt ttaggtacac gggaaaagtg agcaagcagc acagcatcta aagaaaaaga
     3001 aaacactttg gaactttgga taatcttgat atcttctgcg tataattggt cgattcgttc
     3061 gtcttcgtga agcatggatt ccctccaaaa tgaaaaaaat agtcgtctgt attatactat
     3121 ttttattaaa aagaaccaag gtggcttgtc aaacctctat tttttatgca tactatgacc
     3181 ataagaaggg aaaggaagag actatgtttt atcgatttat gcgcggggta gttcgagtac
     3241 tattagcctt agttaatgga aatgcccgct atgagaataa agaagtttta ccgcaagatg
     3301 agaattacat actagttgct ccccatcgta cttggtggga cccattatat ctggcggtag
     3361 ctgcagcacc caaggaattc agctttatgg caaaagaaga actgttcaaa aatccagttc
     3421 tgcgatttat attaactcga tccaatgctt ttccagttaa aagagaaaat ccaggaccaa
     3481 gcgtcattaa aacacctgta aagattttga aatcgacaaa tttgagtttg atcatgtttc
     3541 ccagtgggac aagacatgct acagaactta aaggcggaat ggcattgatt tccagaatgt
     3601 ctaaagcgaa aatcgtgcca gctgtatatc aaggtccgtt gacacttggg gatctgttca
     3661 agcgtcagag agtgacagtg cgtttcggcg aaccaatcga cttatcagat atcaagaaaa
     3721 cggataaaga aggtatggcg gagatcgaac gacgaacaca agcagctttt gacaaattgg
     3781 acaaagaagt gaaccctgat ttcaaatatg aagtcaaaaa aaaataaaat gataagttta
     3841 tgcgtgggcg gtcttttgaa tctactgtcc tgttcagttc agactttttc aatgaaaacg
     3901 agggagagac gtcaagtctg ccctcttttt gcgttccaat atgtaaaata gaagacaggt
     3961 gtcatgtaat ggatcaaagt gttataataa ttagaaggac attttttgag gtgattgaat
     4021 gcgtttttta catacagccg attggcatat cggaaaaaaa ctacaaggtt atgacttgat
     4081 tgaagaccag cgtcatgtac tatcttctat actagatatc gcgaaagaag aaaaagtaga
     4141 ggcaatcgtg attgccggag acttatatga taggagtgtc ccagctgttg atgccgtaga
     4201 attatacaat gaattgatgg tcgattggaa tttaaaggaa aagatcccga tttttgctat
     4261 atcaggaaat cacgatagca gtacgagact ttcagcgggt acgccatggt tcagttattc
     4321 gaactatttt ttgcgaacac agctagcaga agcatttgaa ccagtcgagt ttaaaaacgt
     4381 acaatttttc tttcttcctt actttgaacc aattgcagcg agattatatt ttgaagatga
     4441 caatatccga acgatcaagc aagcggtagc caaggtgata gataaaatgc aggagctatt
     4501 tgattcagaa aaaaaacaag tattgattag ccattttttt gtaatgggaa gtgtaaaatc
     4561 tgattcggaa acgaaaatcg aagtaggcgg gttggattcg atccctggtg aactacttac
     4621 accgtttgat tatgtagcac taggacattt gcataatcaa gctgctttga gacaggataa
     4681 tgctcgctat agcggttccc cattgaaatt ctctttatct gagatcaacc aaaaaaaagg
     4741 cgtatggatc gttgacctga caacaggcct ggagttgaat tttagggaga tcgaacccct
     4801 gcatgatatt gaagaaataa cagcgagttt caaagaattg ctggatcctg ccttttatca
     4861 gtccgtcaat cgggagaatt acatctatat ctatctcact gatagaacga tcatcccgaa
     4921 tatgatgaac cagcttcggc aaatatatcc tcgtattttg gggattgaac gggcaaacgg
     4981 tcgagcagaa caaacaaagc gcaaacgaaa agtactagaa atgaaagatc ctcaaaaatt
     5041 agcagaagca ttttttagtg aaatgacgga agagcagcta actgcaaacc aagaaaaatg
     5101 gctcggagaa acgttgcaag aaatcatgga aatggaatga tcaagaatga aaccgaaaaa
     5161 attacaactt aaaaattttg gtccttttat caatgagacc gtagattttt cacgtttgac
     5221 agaagcaccg ctttttttga tcagcgggaa gacaggagct ggcaaaacga cgatttttga
     5281 tgggatcacc ttcgcacttt ttggtgaaac ttccggaaga cttcgctctg gaaaagagat
     5341 gcgttctttg tttgctacac cagacgaaga aacaagcgtg actttttctt ttgaacatca
     5401 gcagatgagc tatgaaatcg aacgaaaacc agaacaagtt ttagcaaaac gaaaaggaaa
     5461 cggaacaaaa aaacaggcag ccaaagtaac gttgaccatt tttgatggga aaggaaaaga
     5521 aataaaacaa atcacaaaac gtacagaagc agatcagttg atcagagaac tgatgaatct
     5581 cgatgcgaaa cagttttctc aaatcgtttt actaccccaa ggagaattcc gtaatttttt
     5641 ggtttcatca agcagtgata aagagactgt tttacgtcat ttattcggta ctcaattttt
     5701 ccaacagttc aatgagcgat taaaagaaaa agcaaaacaa cagcaacaaa aattggatca
     5761 tttagagcaa gagctgcatc ttctgcaaaa aaggtttgtt ttagtcaaag aagaggaacc
     5821 agcagcaggg tttgaagccg ttttagccca atggacaaac catcaggcaa tactcgaaaa
     5881 acaaatcacg gaagaaaaag aagctctaac ggaacttcag gaaaaacaaa agcaactaga
     5941 aaattcctac tatcgttttg aaagcttgaa aaaaagttat gaagaaaaaa cgatattaat
     6001 aaacaagcaa aaggaattag ctgctcaaaa agacgaaata gaggaaaaga aacgatggat
     6061 tgggtattat gaatttacgc aacgattagt cgaaccaatc cagcgcctac aagaaataaa
     6121 agttgaaaaa atagaactgt cacaaaaaag tagtgagcaa acaaagaaac tagcgatgac
     6181 tacgcagaat tatgaagagt ggcaaaaaaa tgaagcaaac cgcgtacgcc gacaaacaaa
     6241 aatcaaagaa gctgctcaaa aactgcaaaa caaacagatg atggtgccaa ttgtcgaatc
     6301 cttacgtagt aaaaaacaag acgcagaaca attaacaatt tcattggata aagaagaatt
     6361 gaatgccgag aagctgtatc aaaatctcgc tatctatcag gaaagaactc gaattcttca
     6421 agaaaattta ctggatcagg aaaaactgca agaagatcgt cttgcttttg gacagttaaa
     6481 atatctagat gaaaagcagc aagaaataga aaaacaaaaa gcaaaagaca ctgggtcgct
     6541 cgcacagata agagaagaac tcaaaaaagc aactgaagaa ataacaaaaa atagtcagct
     6601 attgaaagaa cagacagaac aagcagaaca gctgaaaagc caatgggcaa agcagcaaat
     6661 tgctcgtctt cagctttttt tgcgtccagg ggagccatgt cttgtttgtg gatcaacgga
     6721 acaccccata caacaattaa aacacgaaca gccaaataag gaagatatct taaaagtaga
     6781 acaacaactg gcagaagctg agtctgctgt agaaagaact cagcaaatca ttgccaaaag
     6841 tgaagcacat gaaaataatc ttcaacaaca attatcagat ttagaacaaa aaatcatggc
     6901 aacgaatata caattggctg aacaagaaga acacttgcaa caggctttga aaaaggcatt
     6961 tcctgagata acagaacaaa aaacaaaaac aactcttgta gtaatcgaac aatctttaga
     7021 tagacgaaaa agcaagatgg ccaatgcaag aatagagctg gaagaaataa gacaacaagc
     7081 agaaaagctc gaacaacaat tagctgtcta caaacagcag cagacagaaa aaaataatca
     7141 gctccagcaa ctgcaaggag aaatcaaaag tttagaacaa caattgcaag atacggatag
     7201 taactgttta gaagaggaga tacgacgact tgaaaaacag ttagaagaag actcgaatca
     7261 actagctgtt gagcagaaag caggagtgca attgttgcaa gatcttcata cgcttcggac
     7321 acagcttaat cttcaacatg aacaaataga aaaaatagat gaaaaaatag aaaaggaaca
     7381 agaaagaata gctaaaaagt tagaaagcca gtcgtatttt tcaacaattg aagaaatctg
     7441 tgcatttgca caacagcaag cacaatataa agaaaaccaa cgtacagtta cttcttatca
     7501 tgaagagcaa ttagtgacag ctgaccggct gcgtcaatta gaaaatatcc agctaactga
     7561 gacattccca gatacagaag agatcagcca acagttagaa gaaataagac gtatgatcga
     7621 aataaagcaa gaaaagatat acggtttaca agaacaaaaa attagtaatc aaaaaatcta
     7681 cgatgaatgt ctttcgattt atcacacaag ccagacgaaa ttagatgagc ttacgcaatt
     7741 acagcagctt tcgcaaacat tcaacggaga aaatcctaag aagactagtt tggaacggta
     7801 tgtgcttcag gtttatcttc aagaagtgtt acaagtagca aatgatcatc tccaacgttt
     7861 aacgaaaaat cgttatcaat ttgagctagc ggataccatt ggcagttatc gagggaaaac
     7921 cggattagaa atcaatatct atgatgatca ggcaggtatg tctcgaagtg cgcataccct
     7981 gtctggcggt gagagtttta ttgcagcact ttctttagca ttagctcttg ccgatgtgat
     8041 ccagacacaa gctggtggtg tagcaatcga tgcattgttt attgatgaag gatttgggtc
     8101 attagatgaa gaagctttgg aaatggcaat ggaagcttta gaaacgatcg aaagtgaagg
     8161 aagaatgatc ggcatcatta gtcatgtaag agaattgaaa gaacgagtat tacaacaaat
     8221 aagaattgat acagaaggta gcggacaaag caaaatacgc tatcaacttg gataaaaagg
     8281 gagattggta actatgaaat ggagtattcc agaaaaagtc attgcacgtg gacgtcaata
     8341 cttgcaagaa ggccgagtgt tatcggttgt ccctgaccaa gaagaaaacg tgtggcatgc
     8401 agaagtcttg ggcagtgaat tgtatatggt tgatttggat ggtacagcaa aagagataga
     8461 ttactgccaa tgtccctatt gggaggaaca taaatattgt aagcatacag ttgctgtgga
     8521 actttattta cggtcgaaaa atctatctcg tatcatgaaa aaagaccagc caatgctaga
     8581 accggtaaaa gcaacaagtg agggagaaat cattgcaaaa ggtttttctc gtctaaaaac
     8641 acaaaacaat cctcaagcgg tgcaaccttt gattattgaa taccaagtag aaacgattga
     8701 gactaatcag tatcacccag agctttctat tttggcactt tacttgcgta tcggttcatt
     8761 ggcatcaccg aaaaaaacat atatcgtaaa aaacatctat gattttttac aagcttacac
     8821 agaaaaagaa acttacactg ttaataaaca gtaccagttt cgtctagata aacgggcgtt
     8881 tcaaaaagaa gatcaaaaaa tactcacgca tcttgcagga agtgctcaga cgcatcaatt
     8941 attaggtacg aacggcttac aagtcaaagg aaaactagat aaaagatatc tgcttctccc
     9001 agtagaacaa gcccgcttcc tgttagagca gatgaatcag actacacgtt tgcatctgca
     9061 gataaatgaa caaaacgatc aaggaataca attttccgat caaaacaaac ctttatcatt
     9121 caaagtggaa aaatttggag aagattattt gctgcaagca ttgaatgatt ttgatttttt
     9181 ctttatgcat tatcagtggg gagttatcca agggacattt tattctctga cgaaatatca
     9241 acaaacggtt tatcaaactt ggaaacaatt gattcgccga ttggagaaac cagaagtagt
     9301 gttcaagaaa aaagaattac cgtctttgtt taaagagatt atccctctgc tgtcagagat
     9361 cggcgacgta gctattgccc ctgcagtaga agaggaagta gcagatatcc ccgtcgaatt
     9421 tattttcttt ttccgaaaag caaaaggaaa aatccaagct cgagtcgatt tcgtctatgg
     9481 tgaagtcatc tattcgactg acccgaagca tgaggtctcg acagaacagg cttttcaggt
     9541 gttacgtgac acagtagaag agaaacgagt acttgatcta ttcagaatgt atcgctacca
     9601 gaaaaatgaa actggttatg aacgtccatt gccaagcggg gaagaactat atgccttttt
     9661 ccgcacagaa ctatctgtct tccatcagtt tggtgaagtc agacttggaa gaaaattgcg
     9721 agagttgtat ttagatgcgc ataaacatca accgaaaatc gaagtaaccg agaccggttc
     9781 atggctcgat attcgattcg atgtgacagg gatagaggag caagaaatcg atcatgtcct
     9841 gcaaagcttg ctgagaaatg atgcttttta tacattagaa aatggtcagg ttctttcttt
     9901 tgattcagaa gaatttcaac aaacaagcca agtgttgcaa caattacggg aatcgatacg
     9961 tacagaagaa ggaacgatcc atgtacctaa aaatcaagga ttgatcattc aaaatcaatt
    10021 ggaaaaaagc aatgctactt ttagtgaatc gttccaaacg atggtacaag atctcattca
    10081 tccagaaaga taccaggcac agcttcccaa aggattgaat gcaacgatga gagattatca
    10141 aaagcaaggt tttcgatggt tgaagatgtt aggtcattat cagtttggcg gtattttagc
    10201 cgatgaaatg ggacttggga aaacactaca gacaattgcc tttttacttt cggaaaaaga
    10261 agaacggaaa agcttctctg ctttgatcgt agcgcctgcg agcttaattt acaactggca
    10321 ggcagaggtg agaaaattcg ccccttcttt aagtatccaa gtaatcaatg ggaataaaaa
    10381 agaacgtgaa gaattactgg ctaaagacac agatatccga gtgacttctt atgccagctt
    10441 aagacaggat ctagctaatt atcagtcgca aaaaattgat tatttgattc ttgatgaggc
    10501 acaaatggtc aaaaatagca gtacgaaaac tgcacaagca ttgagagaat tagctgtacc
    10561 tcagcggttt gcactgagtg ggacaccaat cgagaataat ttagaagaat tgtggtcatt
    10621 gtttgcaacg attatgccag gatttttccc gactaaaaca aaattcaggg aacttgccgc
    10681 agaagaaatt gcgcaaatga ttcgaccgtt tattttacgg cgagataaac aaactgtgtt
    10741 gaaagacttg ccggaaaaaa cagagatgaa tctatattct gcacttacag aagaacaaaa
    10801 aactgtttat ttagcttact tgcgtcaaat gcgtgaagaa atcacatcaa tggattctga
    10861 agcattcaag aaaaaccgga tcgggatact tgctggtctg acaagactgc gacaaatttg
    10921 ctgcgatcct cgtctgttta ttgaagatta ccaaggcggt tccggcaagc tcgaacaagt
    10981 aaaagatcta ctggtagctg ccaaagagaa caaacgaaga gttttgctgt tttctcaatt
    11041 tacagggatg ctgactatct tgcaagaaga gttagccgag ctagggatct cgacatttta
    11101 tttaagaggc agtacgaaac cacaagatcg gttaagtatg gtagacgcat ttaatgctgg
    11161 cgaaaaagat gtttttctga tttccttaaa ggctggggga actggattga atctcacggg
    11221 agcagatacg gtcatcttgt atgacctttg gtggaatcct gccattgaag aacaagctgc
    11281 tggacgtgca catcggatcg gacaaaaaaa tgtggtagaa gtgtggcgta tgatctctga
    11341 aggaacgatt gaggaacgca tggattcact ccagcaagaa aaacgcgaac tgttccaaaa
    11401 agtgatccaa ggtaatgaag aacagttgac gaaactgact gaagaagaca tccgcatgat
    11461 tttaagtgta ggagaaatcg aggagtaaga gactcgaaaa agcgttcagt aatgggtaag
    11521 aaagcctttc taaagaagcg attgtttatt tgatttaaaa aatagcaagg aggctgggat
    11581 tccattttat cccagcttcc ttgctatcat ttttttattt ctatttttaa aggttataca
    11641 aagattgtct tcattgtctc taacagctga tcaaaagaag aaatctcgat atcgcaaatt
    11701 ggagtctgtt caattttgcg ttctcgatgg ttgaaccaaa gcgcttgcca gttagcactt
    11761 tttgcaccta gaacatcatt gtcgtaatta tcgccaacgt ataaggtctc ttctggaagc
    11821 atatgaaatt ctttctcagc cagctgaaaa atctcttttt ctggtttttg gaaacctgtt
    11881 gcttgagaaa tgagcatatt gcgggaagga atccaattgt tcaattgaag ttgcttcagt
    11941 ttttttgtct gatggtcagt cggcccattt gtgataattc ctaatgggac attcttttcc
    12001 ttcaagaaat cgagggtttt tttgacctcc tcatgcaagc agatattatc aagttcttcc
    12061 tcgtaaattt tttgaaataa gaggccatct tcttccgtaa tatgagggta gtctaaatct
    12121 tttagtgatt ggctgatccg atgtgctcgc atatattcta gtgtccaatc tcctgccatt
    12181 acttttggga agttttcatc actatgatgg cggaaccgga tatatagtgg atgcatatct
    12241 gcatctgaaa caagcggaac gactcttttg accgcgtttc gaaatggctg ctgctgatca
    12301 tacatcgtat catcaacatc aaacacaact gatttaatca aaattttaca cgcctttcgg
    12361 ttttcattga ttttttcaca aatccttgac tattgtaaca gaaaaaattg tctaaaaaag
    12421 ctgataaata aaggtttttc ttactatatt tctatttcct tgacagaata aggtttttat
    12481 atttcagttt ttttcactaa aaatcgtgaa acacgtcaat aagtttgatg aacaaattaa
    12541 tcattatgg
//
LOCUS       DAZUKC010000071        12188 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000071 DAZUKC010000000
VERSION     DAZUKC010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.073"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..314)
                     /locus_tag="ACG80T_002041"
     CDS             complement(150..314)
                     /locus_tag="ACG80T_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841675.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            609..1937
                     /locus_tag="ACG80T_002042"
     CDS             609..1937
                     /locus_tag="ACG80T_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFS4841676.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2139..2972
                     /locus_tag="ACG80T_002043"
     CDS             2139..2972
                     /locus_tag="ACG80T_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFS4841677.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2988..3725
                     /locus_tag="ACG80T_002044"
     CDS             2988..3725
                     /locus_tag="ACG80T_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFS4841678.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3880..4848)
                     /locus_tag="ACG80T_002045"
     CDS             complement(3880..4848)
                     /locus_tag="ACG80T_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFS4841679.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5080..5913
                     /locus_tag="ACG80T_002046"
     CDS             5080..5913
                     /locus_tag="ACG80T_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFS4841680.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5947..6786
                     /locus_tag="ACG80T_002047"
     CDS             5947..6786
                     /locus_tag="ACG80T_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4841681.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6812..7372
                     /locus_tag="ACG80T_002048"
     CDS             6812..7372
                     /locus_tag="ACG80T_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4841682.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7571..9292
                     /locus_tag="ACG80T_002049"
     CDS             7571..9292
                     /locus_tag="ACG80T_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFS4841683.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9457..10599
                     /locus_tag="ACG80T_002050"
     CDS             9457..10599
                     /locus_tag="ACG80T_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFS4841684.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10615..11826
                     /gene="thiI"
                     /locus_tag="ACG80T_002051"
     CDS             10615..11826
                     /gene="thiI"
                     /locus_tag="ACG80T_002051"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFS4841685.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4152 a         2046 c         2532 g         3458 t
ORIGIN      
        1 ctactgccgg agtaaataaa gcttatgcaa agaaaaagaa atccaatcac ttgggtttct
       61 ttttctttta ttattatgat gaatcaagct ttctgttttt gtcacgtatg ttgtaatctt
      121 tacgaaagag tagagttggc tactcataat caaccatact tcccatcatc gctaaatacg
      181 aaataaatca gcattcctac accaatcaaa gcacaagctg caccaagaaa agggatgaca
      241 ttcccaaagt tatctgtatt cataagtatg ccaccaccca caaacaaaaa gccgccagca
      301 atcaggtttt tcattttatt catctccttt cttacgttat agtatttcct tctttcaatg
      361 agtattttga aagctatctt attgtatgat gttcttctag gcctcttctt tattatacag
      421 gccattttcg ttatctcata gaaatattcc tttttatctg aaaactattg tataccgtat
      481 gacatttttc ttcatgaagt tgtaagtata ttctcattca aatgaaaatt tcatcataga
      541 agagattgta tttctaaatt cttaagagta aaatacgaag tataacttgg atttggaagg
      601 atgattggat gaaagctatt acgcctgata tgacaacaaa atttaacaaa cagtttcaag
      661 caaatatcaa acagcaggca ttgcagcggg cagttgtgaa gaacggaatt actgcttctg
      721 ctgaaaatgt ttcagcacat gtggagaata caccggtatt ttctatagat ttggctacgg
      781 ggaaagtggc taatcaaatg caatcggggc gttgctggat gtttgctgca ctgaacacat
      841 tccgtcataa gatgctgaat caattccatt taaaagagtt tgaattatca caaaattaca
      901 cttttttctg ggataaatat gaaaaggcaa attatttcta tgaaaacatc attgcaacag
      961 gaaaagaggc aatcgatagc cggaaagtag ctttcttact cgctactcct caacaagatg
     1021 gcggccaatg ggatatgatc gtttcattat tccaaaaata tggcgttgtg cctaaatcgg
     1081 tgatgccgga aagtgcgaat agttcaaatt ctcgagattt gaataactac ttgaacaaat
     1141 tactgcgtaa agatgcagta gtgttacgga aaatggttgc tcaaggagaa acattagacg
     1201 ctattgaaga aaagaaagaa gaaatgctag aaagcattta taacttctta gctatctcat
     1261 taggaacacc tccaaaagaa ttcgattttg agtaccgcga tgaagaaaag aactatcatc
     1321 ttgaccgagg actgacacca caaatttttt atgataaata catcggtgta aaattagatg
     1381 attatgttag cgtgatcaat gcaccaacaa aagacaaacc attcaatcgt tcttatacag
     1441 tagagatgct aggaaatgtc gttggcggga aagaagtcaa atatctgaat gttgatatgg
     1501 aaactttcaa aaaattagcg attgctcagc ttgaagaagg agaatctgtt tggtttggtt
     1561 gtgatgtagg tcaatcttct actagaacta gtggcatcat ggccttagat gcttattcaa
     1621 tggatgatct atttgatacg gattttacaa tgactaaagc tgaacgttta gattttggag
     1681 aaagtctgat gacccatgcg atggtattga ccggtgttga cttagtagac gggcaatcaa
     1741 caaaatggaa agtcgaaaat agctggggcg aaaaagttgg aaagaatggc ttctttgtta
     1801 tgagtgatgc ctggatggac gaatatacgt accaaatcgt tgtaagaaaa gatttattga
     1861 cgaaagagca attaaatgcg ttcaatgaag aaccaattgt tctatctccg tgggatccaa
     1921 tgggtgcttt agcatagacg agtatttcaa tgaagagtat gtagtaaaga taaaaagagc
     1981 tggcttaagc cagctctttt tatctttaaa gtccattccc tgttttttat ggtccctgtg
     2041 ctataattct aaataaagga taaggattgg aaataatcct tttatcatta aaaatatgat
     2101 gacgaagtag aataaaattg ggagtagggg ggaaagtcat ggatatccaa actgttttat
     2161 ctgatctgaa attaaatggt aaagtgattc cagttgttgg tggcgatgtt aatcagacct
     2221 atcgtataaa aaccgaacat cgtgcttatt ttttaaaaat ccatccgaat gtaaaaaaag
     2281 gattttttga agcagaagta gacggattaa aagaactgtc agcattcgta cgtgtaccgg
     2341 atacgtatat gctaggtgaa acaagtgaag gcgcttattt gttaatggaa tggatcgagc
     2401 cgggaaaagg ggatcaacga gatcttgctg cagctttagc taatctccat cagcaaacag
     2461 cgccacaatt tggttttcgt aaagataact atttgggtac acttgttcag aaaaattctt
     2521 ttgaagaaga ttggtggact tttttcttta aagatcggct tgaatcccag atttctttgg
     2581 cagaagaaac gaatcgatgg aatgtacagc gtcaagaaaa gtatctgcga tttaaagaac
     2641 gtgtattgaa atcggttgaa ccaaaaaaga tcactccacg cctcttgcac ggagacctgt
     2701 ggagtggtaa tgtgttcttt gaccagcaag ggcatccagt ttttgtcgac ccagctgttt
     2761 cttatgggaa tcgggaacaa gatattgcaa tgagccagtt attcggtggt ttccgaccgg
     2821 aatttttaga tgcttatcaa actatatttc cattagaaaa gggatggaaa gaccgactgc
     2881 cgatttacca gttatattat cttttggcac atttgaatat gtttggagaa agttacggtt
     2941 cccaagtaga tcaattacta gaaaattttt aagaggaggg cgtgtttttg gcaaaaaaat
     3001 ataccaaaaa gcatgaagtt tcatattatg agtgtgacat caaccaaacg atgacatttc
     3061 cttcgatgtt aggagtagtt atcaaaacgt ctgaagatca aagcgaccag ttgggaagag
     3121 gcagtgaatt cgtcaatagc tttggactta cttgggtcat cacgaattat tccatggaga
     3181 ttaatcgtct ccctagagtc ggtgaagtaa tctcagtaac gactcaagcc atggagtaca
     3241 ataagttttt ctgttatcgg aacttctgga tccatgatga agatgggaaa gaattagtca
     3301 aaatagaatc tgttttcgta ttgatggatt ttgtgaatcg aaaaatgagc agtgttaatg
     3361 aagagatcat tgctcctttt gagagtgaga agatcaagaa aatcaagcgt caggaaaaaa
     3421 ttgaaaagat tgattcgggc ttcatgttgc catatcgtgt tcgtttttat gatatcgata
     3481 gtaatcagca tgttaataat gcgatgtatt ttaattggat cattgacgta ttaggatatg
     3541 actttttgac tacgcatgtt ccagaaaaag taattattcg atttgataaa gaagtagagt
     3601 acgggaatga gatcgaaagc cattttgaac aaatccctat cgaaaatggt gtcaaaacaa
     3661 aacatgaaat ccgaatgcaa gaacagctgt attgtgaagc aaatattata tggaaaaata
     3721 aataaaaaag aggttgtgac agaagggggc aacttcaaga aataagaaga aatttacgaa
     3781 aattgtttct caaatttttg taaattttcg acttagtttt cgaagaagct atttttgtca
     3841 cagcctctct ttttaagttt agctattaga taatgatgtt tagttttgga taaacgaaat
     3901 caattcttca cccgacaaac catagtttac agcaatcaat agttttaaac gtgctttttg
     3961 actattaata ctgttacaga aaataatccc cattttttct agttcttttc ccccgccttc
     4021 ataatcataa acaggctctg cgattccatt gaaacaacga gaaacaagta ctacagggat
     4081 tttttggaca agaagctttt ccaaagcagc taaggcttgt ggagggagat tccccgcacc
     4141 taaagcttcg attaccaaac cgtctacttt cgtatgggcg atggcttcca ataagtcccc
     4201 ttgcatccca gcatatgctt taacgatcgg gatagtccca tctacagcgg aaatgtccag
     4261 tcgctttgtt tctaaaagtt cctgcaagaa taaaattcga ttcttagtta ctaagccgat
     4321 gggacccaat gtaggtgttc ggaaagtagc aacatttgtg gtatgggtct ttgtcacgta
     4381 gcgtgcagaa tggatctcat cgttcatgac caccaaaacc cccttatcta gcgcttcttc
     4441 acaacttgcg acacgaatcg cactttcaaa attataaagg ccgtcacttc caagttcatt
     4501 acttgaacgc attgccccag tcaaaacaat tggtaatttg ttgccaatcg ttgtatcaag
     4561 aaaaaaagca gtttcttcta atgtatctgt accgtgtgtg atcaccactc cggatatacc
     4621 tgatgcataa gcggacaaga tacgttcttt taataccaac atgtgagaag gcatgatgtg
     4681 tggtgaggga agattgaaga tatcttcaac taataattct acatgcttgg ggattgtgat
     4741 ttctgcatct aataatgggt ttgccacatc aggactaaca ccgcccgtga gcatgttttc
     4801 tttcatggcg atcgttccgc cggtgtgtag tacaagtatt tttttcattt tacagacttc
     4861 cttacgaata attttgcata gccgttaaat cttattgtat actatataaa agagttttgc
     4921 tatcagtgaa gaataacgaa tagcctaact tatcataaaa aagctgacct tattaaaatg
     4981 gaaaagactc aaccttgacc tcctaataca atggagtatc tggttgagtt tcttttacaa
     5041 gaaagcagcc aaggaaatca atcacgaagg tggccggaaa tgatcagaat cggattaaca
     5101 tcgtttaaag aacatgacaa attaactggt aaaaagaata acactcttta tgaatatgca
     5161 gggtttctac ccttagtgga attagatact gcctattatg gaattccccg aaagtcctca
     5221 gttgagaatt ggataagtga agtacctgaa tcatttcgct ttgtagtcaa agtctatcaa
     5281 ggaatcagcg gtcaaggaga ctggaagcct tattatcaga ccgaagaaga gatgattgcg
     5341 cattttttag atgttatgtc gccaatggta gatgctggaa agatgttttg ctttttagtc
     5401 caattcccct catcattcaa atgtacgaaa gaaaatgtcc agtacttgcg caaacttaga
     5461 aagtggtttg agggttatcc tgtttctata gaattgcggg attattcttg gtacagcgat
     5521 gaatttcttg aacgtacaag aaactttatg tctagagccg ctttttcttt agccatcatc
     5581 gatgagccac agttgctgaa tacaacgatt ccttttgatc catttgtcac caatccgaac
     5641 tttgctcttt ttcgattcca tggtaggaat gcgcaaggat ggcaagcaaa tgataaagag
     5701 tggcgtaaaa aaagaacgtt atatcgttat tcagaaaaag aactgcaaga actagcacat
     5761 gaagtcaaag tagttgattc taagacaaaa gaggtaggtg tgatttttaa taataattct
     5821 ggtggtgatg cggctgaaaa tgcattagca ctgcaaaaaa tattaaatat caagccagat
     5881 ggactcaatc caacccaaat ggatctgttt taagggctta ttttattcaa gaaaggggcg
     5941 aagtctatga tcaaagctat tttttttgat attgacggaa cattggtcac caatcgttca
     6001 aaagcgttgg aatcaacaaa acaagcaatt gaatatgctc ggaaaaacgg cattttatgc
     6061 ggtgtagcaa cggggcgcag cccagtaaag atcaaagaaa tcattgatga attggaatta
     6121 gatatgtatg tcgtgtataa tggtcaactg gtttttactg cagatcgcac gattatcgac
     6181 cacccttttg aacaaaaagt attggaacat attgttgaat ttgcagatga gaaccatcga
     6241 caaatcgttt ttggtgcgag aaatcggtta gatggatcaa cgacgatgct attaggacaa
     6301 tcgattttta tcaagcgact agtcagcttt ttaccgcgta aattcccggt acgtctgatg
     6361 aaaaaaatcc tccaggtttt tagccctcac cgacaaaagg atcgttatga aacgttagag
     6421 atattaaaag aaccaattta tcaatgtata ttgctcagtc cggagtcaga acagaaaaag
     6481 ttagaacatc gctttcccga gtgtacgttt cagcgttcaa atacgtatac tgtagatatc
     6541 atcccaaaag gtggttcaaa attactaggc atccaagctt tcgcaaatgc tgtcgaaatt
     6601 gaaatggaag aaattatggc attcggcgat cattacaatg atatcgagat gctgaaaggt
     6661 gtaggaattg gagtggccat ggggaatgct cagatcgaag taaaacatgc tgcagacttt
     6721 gtgactcaat caaatgaaga agatgggata tacgaaggac tgaaacactt cggtctgatt
     6781 tattagtcaa tgaaagaagg aaaaaatcag tatggaagca gagacacctt ttcaaaaagt
     6841 agaaacattc cacaaaactt ttgatccaag aagaccagag atacccacgg cttttacaga
     6901 aaatgaagct catacacgtg ctgcgttcaa aatagaagaa cttgttgaat ttgttgcagc
     6961 tacttcacct aataaagaac aactgcatga agctgttatg ctgctgcacg aagcattgga
     7021 taaagcagaa gaaaaaatac ttggtaaaac acagtgggga agtgatccgc tggttgaaca
     7081 agtagatgca ctgacagata tcctttactt tacttatggt tctttttcat tactaggaat
     7141 agtgcctgat cgaatattcg aaatcgtcca tcaggcaaat atggggaaat tatttccaga
     7201 tggaaaacct cattatgacc ctgtaacaaa taaagtgttg aaacctgaaa actggcagat
     7261 ggaatatgct ccagaaccta aaatcgcggc agaaatccaa cgtcagattc aagaagcgat
     7321 tgctaagaag aaatccgctg atataggcat gtgcacaaat gaaaaagagt gatcagagtt
     7381 tcactatttt tgttatcttt atacgatgga aaaacaaaag attttcgttt aagttagtga
     7441 atgcatgaaa atagtatgct ataatctaga atagatgtct tttgcacgag agacgtatta
     7501 tttggattta tgccaagcag aacgccatct gttgcataaa agatgcatac tgaaaacgga
     7561 ggaaccataa atgggaaata atgttatcat cggaatcgtc attgcaatca ttatagtcgc
     7621 cattgtcgtc tttttggtta gttacttgat gaagaaaaaa aaccaagatc gattggatgc
     7681 cttagaaaaa cgcaaggaag aattatttga cttgccagtg attgaagaag tcgatgatgt
     7741 taaaaaaatg catctggttg ggcaaagcca aaataccttt cgtgaatgga gtcaaaaatg
     7801 gaatgacata tctacttcct cgttcgctga actagaaagt caaattttcg aagtggaaaa
     7861 cctcaatgaa agttttcggt tcttaaaagg aaaaaaagca gttgttgaag cagaagctac
     7921 aatggaccaa atggagaaac aagtgacgga aatccgtgaa ggtctaaagg aattgcggga
     7981 aacggaagaa cgcaactcac tagctgttca gcaggcactt gacgtctatg aagaactgaa
     8041 agaaaaagtc agagaagacg agcatgaata cggaccagct ctttttgaat tgcaaaaaca
     8101 gatcaaagtg atcgaaaaag aatttacgca gtttgtgact ttaaacactt ctggagaccc
     8161 tgttgaagcc cgcgaggttt tggataaagc agaaaaacat acgtatgaag tggaagctct
     8221 gatgaaaaaa attccaccgc tgtatgagga gctccatacc actttccctg aacaacttga
     8281 agaaatcagt gatacttacg agaaattgat ggaagaacaa tatgtattcc cagaagaaga
     8341 tcttgctgaa gatatcgcaa aagtaagtcg aagaattgaa aattcattag ccaatcttga
     8401 aaaaacagaa gttgaaacgg tcgaatttga aaaccgtgaa acagctgatt tgattgattc
     8461 gttatatgat atcttagaac gggaaatgga agctcagaga tatgtaaaaa ctaaccaatc
     8521 aacgattgca gagtatatca aacatacaac aaagaataac cgtcaattat tgattgagct
     8581 ggatcacaca tctcaaagct atacattgaa ccacaatgaa attggccgtg tacgaggttt
     8641 ccaaaccgaa gtggaagaaa tggaacgtca aaatgaacag atgattccac agatccgtca
     8701 acacgaaatt ccttattcag aagtgcgcac gttctataaa acagtcttca aagtcttaga
     8761 agatatcgaa aagcaacaag tagagatcga cgactcatta catgaattga gaaaaggcga
     8821 aaaagaagcg caagaaaaga tcgatacatt cgaattcaaa ttgcgcagtt tgaaacgttt
     8881 tgtagaaaaa caacgtttgc ctggcttgcc gaatgattac ctagaattct tttttgttgc
     8941 tactgaccgt attgaagaac taagtgttgt cttgaataaa attcgagtaa acatggaaga
     9001 ggtcaatcgt ttagttgctc tatgtgaaga agatctagaa ttattagata agaagacaca
     9061 tgatttagta gatgcagcag cattaacgga gcaaatgctc caatatgcaa atcgttatcg
     9121 tcatacacac gaagaagtga gagaagcaat tgataaaagc tactatttat tcaataaaga
     9181 atatcactat caagaagcat tagacgaaat cggtacagca ttagagcgag tagaacctgg
     9241 tgctttcaaa cgcatcgaag atttttattt taatcatcca gacttagtat aaagcaaaaa
     9301 agttagtaaa ccagcaagag caagacggat tattgtcttg ttcttgtctt tttttgtata
     9361 tttgaaaatt gaaacaagtg aaaatttcac ctgctcttga aagcaccttg gacaaccgtt
     9421 ataatagaaa agaatttcaa aaaagagggg tttcttatga tatattttga taacagtgca
     9481 acaaccccaa tctttccgca aagcttagat gcatatgtaa aaaccagtca gcggattatt
     9541 gggaatccat caagcttgca tgatttaggc aatcaagcta accggttgtt gcagcaagca
     9601 agaaagcaga tcgctgattt gatccatgta gaaccagaag aaatctattt tacttcaggc
     9661 ggaacagaag gagataactg ggtattaaaa ggaacgatga tcgaaaaaag agaatatggg
     9721 aatcatatga tcatttcagg agtcgaacat ccggccgtca gcgagacagc agaacaatta
     9781 aaagaattag gaatcgaagt aagtatcgcc ccagtggata aacgcggatt tgtacgtgtc
     9841 gacgaattga aagaattaat aagaaaagag acagtattag tctctgttat ggctgtgaat
     9901 aatgaagtag gatcgattca gcccatccag gaaatcagtg atcttttgga tgattttcct
     9961 aaaattcatt tccatgtaga tgctgttcag gcgattggaa aagtaccatt ctcgcaatgg
    10021 ttgacagaaa gagttgattt tgcaacattt tcagctcata aattccatgg cccacgggga
    10081 actggtttca tttattggaa aaagggtcgt cgtttagcac cattactgac aggtggaggg
    10141 caagaaagaa atcagcgaag tgggacagaa aatgttccag gtattgtggc tatggttaaa
    10201 gctttacgtt tatcattaga gataaaggaa caaaaacctg agcagacaca acgcttaaaa
    10261 gtagctctaa tcaatgcgtt gaagacgtat gagaaagtga cgattttttc agaaggaact
    10321 gactatgcac cgcatatttt atgttttgct ctaaaaggaa ttcgcggcga agttctcgtc
    10381 catgccttag aagaaaaaca aatatttgtt tctactacta gtgcctgctc ctcacgtaaa
    10441 catatggcaa gcagtacatt gcacgcaatg catgtaccaa atgatctggc tacatctgct
    10501 gttcgaatca gcttagatga aaacaattcg cttgcagaag tcgagcaatt tatgattatt
    10561 ttcaagcagc tttataagaa attttctgca atcaattaaa agaggagtgt tcttgtgaaa
    10621 tacagtgaaa ttatggtccg ttatggggaa ctttcgacaa aaggaaagaa tcgaaaaagc
    10681 tttatcatgc aactagcaca aaatgtcaga caagctttga aagactttcc agaaatcaaa
    10741 atccatgctg atcgggaccg gatgcatctt ttattgaatg gcgcagacgg gcagctagtt
    10801 attccaaaac tagcaaaaat atttgggatc caaaatttct ctccaagtat tcgtgtagaa
    10861 aaagatgtcg aagttttgaa acaagctgtt caagacatca tgaaagaaat ttatactggg
    10921 aaagaaacat ttaaaattat cgctaaaaga agcgatcacc aatttgaatt gacaagtaat
    10981 gaattgaatc aaacattagg taatgctgtt tttgatattt tcccgcatat ccaagttcaa
    11041 atgcgtcaac cagatattcc attacgtgta gagattcggc gtgatggcgc ttatctatcg
    11101 tatgaaacga tcaagggagc tggaggattg cccgtaggaa caagcggaaa aggaatgctt
    11161 atgctttctg gggggattga ttctcctgta gcagggtact ttgccatgaa gcggggagtg
    11221 gaaattgaag ctgttcattt tgctagtccg ccgtatacaa gtgaacaagc actgcaaaaa
    11281 gcaaaagatc taacagccaa actagcacct tatgtgggaa cgatccattt tatcgaagtg
    11341 ccatttaccg agattcaaga agaaatcaaa aagtcagtgc cacaaggata ttggatgaca
    11401 attactcgtc gaatgatgtt gcgtttgacc gatgaaattc gccgtattcg tcatggttta
    11461 gtcattttga atggtgaatc attaggtcaa gtggcttcac aaactttgca aagtatggtg
    11521 gctatcaatg aagtaacgaa tacgccaatc attcggcctg tagcgacaat ggataaactt
    11581 gaaatcattg aagtagcaga acagatcgat acatttaatc tttctatcca gccttttgaa
    11641 gattgctgta cgatttttgc tcctccacag ccaaaaacac gccctcaact ggaaaaggtc
    11701 catcaatatg aagagcgttt agcaatagat gaaatgatcc aacgagcact agctggcttg
    11761 aagatggaag agatacaagc agaatcgtct aatgaaaaaa ctgaagaatt ttcagaattg
    11821 ctataagagg ttatgaaaat agtgttttga ccagagtatt agagaaacaa aacgaaaaaa
    11881 tagctccatg tattttaccg tttttgaact aatgtcgaag attagctgat tgaataccat
    11941 ttatcaagaa gttatggaaa agccggttct tttttactaa ctgaaaaata aaattttatt
    12001 tagagatcaa gctacagttg ataaatgata taaaaaagag aatttcccat gaaatagcga
    12061 ggttgtgaca gaagtgggca gcttcaagaa ataagaagga atttacgaaa attgcttttc
    12121 aaatttttgt gaaatttcgg cttatttttc gaagaagtta cttctggaac accgtgtatc
    12181 agaggttg
//
LOCUS       DAZUKC010000072        12136 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000072 DAZUKC010000000
VERSION     DAZUKC010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.074"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..610
                     /locus_tag="ACG80T_002052"
     CDS             <1..610
                     /locus_tag="ACG80T_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4841686.1"
                     /translation="KRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWIQLIL
                     DEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSKITVF
                     PKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAYTIQN
                     ATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            723..1001
                     /locus_tag="ACG80T_002053"
     CDS             723..1001
                     /locus_tag="ACG80T_002053"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4841687.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            1119..1355
                     /gene="secG"
                     /locus_tag="ACG80T_002054"
     CDS             1119..1355
                     /gene="secG"
                     /locus_tag="ACG80T_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFS4841688.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            1458..2213
                     /locus_tag="ACG80T_002055"
     CDS             1458..2213
                     /locus_tag="ACG80T_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS4841689.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            2216..4573
                     /gene="rnr"
                     /locus_tag="ACG80T_002056"
     CDS             2216..4573
                     /gene="rnr"
                     /locus_tag="ACG80T_002056"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFS4841690.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            4595..5059
                     /gene="smpB"
                     /locus_tag="ACG80T_002057"
     CDS             4595..5059
                     /gene="smpB"
                     /locus_tag="ACG80T_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFS4841691.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            5361..6080
                     /gene="atpB"
                     /locus_tag="ACG80T_002058"
     CDS             5361..6080
                     /gene="atpB"
                     /locus_tag="ACG80T_002058"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFS4841692.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            6133..6348
                     /gene="atpE"
                     /locus_tag="ACG80T_002059"
     CDS             6133..6348
                     /gene="atpE"
                     /locus_tag="ACG80T_002059"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFS4841693.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            6439..6963
                     /gene="atpF"
                     /locus_tag="ACG80T_002060"
     CDS             6439..6963
                     /gene="atpF"
                     /locus_tag="ACG80T_002060"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFS4841694.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            6950..7492
                     /locus_tag="ACG80T_002061"
     CDS             6950..7492
                     /locus_tag="ACG80T_002061"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFS4841695.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            7515..9071
                     /gene="atpA"
                     /locus_tag="ACG80T_002062"
     CDS             7515..9071
                     /gene="atpA"
                     /locus_tag="ACG80T_002062"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFS4841696.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            9085..9987
                     /locus_tag="ACG80T_002063"
     CDS             9085..9987
                     /locus_tag="ACG80T_002063"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFS4841697.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            10010..11416
                     /gene="atpD"
                     /locus_tag="ACG80T_002064"
     CDS             10010..11416
                     /gene="atpD"
                     /locus_tag="ACG80T_002064"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFS4841698.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            11430..11852
                     /locus_tag="ACG80T_002065"
     CDS             11430..11852
                     /locus_tag="ACG80T_002065"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFS4841699.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4035 a         2155 c         2677 g         3269 t
ORIGIN      
        1 taaaagatat gcagagaaag gattcaacgt tcttgtacct gatttacgtg cgcacggaga
       61 aagtgaagga gaaatcatcg gaatgggatg gctagatcgg ctagatttaa ttgcctggat
      121 ccaactcatc ttagatgaac agccgaatgc gtccattatt ttacacggcg gatcaatggg
      181 agcttctacg atcatgatgg caagtggtga aaaacttcca tcagcggtca aaggatttat
      241 tttagacagc ggatatgttt ctgtttatgc agaattccga tatatgttaa gtaaaatcac
      301 tgtctttcct aaaaaaatgg tcatgagata tgcgaatcat tatgcccaga aatacgccgg
      361 ttactcctta aagcaagcat cagctacgag acagcttggc agcaaccatc tgcctttatt
      421 agtcatccat ggtgaaagag atcattttgt ccctacagaa gcagcgtaca cgattcaaaa
      481 tgctacggca ggggataaag cactgctgct tgttccagaa gcagaacact tggaagcatc
      541 gatgaaagat ccgaaaacct attggacagt gattttttca tttattgaac aaagaataac
      601 gatggattaa ttttctcttc ttttttgtaa gtaattcgag gataaataat tgtttataga
      661 tctggagact gctaaaataa ggtaggaata aaaaaatatc agttatttga ggagtgcggc
      721 atatggaaat caaagaagaa aaaaatcgtc ttgctttatt caatgatgag caacaagaga
      781 ttggagaaat gacatggtca gatgcgggcc cagacatcat gatcattgac catacatttg
      841 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
      901 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
      961 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     1021 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     1081 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     1141 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     1201 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     1261 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     1321 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     1381 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     1441 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     1501 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     1561 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     1621 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     1681 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     1741 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     1801 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     1861 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     1921 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     1981 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     2041 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     2101 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     2161 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     2221 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     2281 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     2341 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     2401 agtgaaactt cctttaaaac caatcttgat cgaaggaaca tttcgagcaa atgaacgagg
     2461 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     2521 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     2581 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     2641 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     2701 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     2761 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     2821 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     2881 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     2941 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     3001 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     3061 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     3121 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     3181 tgtatatttg actgatcgag tgattccgat gatcccgcaa cgtttatcga atggtatctg
     3241 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     3301 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     3361 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     3421 tcagctcgta cctatgttcc aagaaatggg tgaattgcat cgaatccttg aaacgatgcg
     3481 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     3541 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     3601 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     3661 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     3721 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     3781 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     3841 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     3901 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     3961 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     4021 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     4081 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     4141 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     4201 aggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     4261 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacaaaaag tgcgcgtaaa
     4321 agtagtcaaa gctgatccag atgaacgagc agtggatttt gaactcgttt ccgcagaaga
     4381 ggtcactgcg ttggaacgtc caaaagcacg caatggaaaa tctggaaaac gagaaggaaa
     4441 acaagcaggc aaacgctcag aaaaaaatgg caagaagaac aaacgatata gtgatagtaa
     4501 gcaaacgttc ggtaagaaaa aagggaaaaa gccattttac aaagatgtca aaaagaagaa
     4561 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaactaat
     4621 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     4681 ggtcttgcaa ggaacggaga tcaagtcgat acgaaacagc cggatcaatc tgaaagatgg
     4741 atttattcgc gtccgcaacg gggaagcttt cttgcataat gttcatatca gtccttatga
     4801 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaagaa
     4861 acaaatcgtt cgtcttgaaa acgaattgaa aaacactgga atcactgttg ttcctttaaa
     4921 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcaaaag ggaaaaaatc
     4981 gtatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     5041 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     5101 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcac aggttttttc
     5161 ttagatcagg agcaccgtgc atggggaaaa attcgcttcg tttttgactg aaataaaaaa
     5221 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     5281 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     5341 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gtttcacatt ggaccaattt
     5401 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     5461 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     5521 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     5581 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacataggtt
     5641 tagtaacaaa aatcgtctta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     5701 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     5761 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     5821 ttccgatgaa actgattgaa gaatttacaa atttattaac acttgcttta cgtctttatg
     5881 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     5941 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     6001 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     6061 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     6121 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     6181 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     6241 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     6301 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     6361 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     6421 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     6481 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     6541 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     6601 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     6661 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     6721 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     6781 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     6841 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     6901 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     6961 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     7021 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     7081 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     7141 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     7201 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     7261 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     7321 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     7381 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     7441 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     7501 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     7561 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     7621 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     7681 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     7741 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
     7801 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
     7861 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
     7921 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
     7981 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
     8041 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
     8101 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
     8161 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
     8221 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
     8281 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
     8341 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
     8401 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
     8461 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
     8521 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
     8581 cggttgatgc cggactatct gtatctcgtg tagggggttc tgcgcaaatc aaagcaatga
     8641 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
     8701 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
     8761 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
     8821 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
     8881 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
     8941 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
     9001 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
     9061 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
     9121 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
     9181 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
     9241 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
     9301 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
     9361 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
     9421 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
     9481 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
     9541 tctgatcagc caagctttga tgaagtccgc aaaatcgtaa atatggcaac aactatgttc
     9601 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
     9661 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
     9721 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
     9781 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
     9841 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
     9901 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
     9961 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    10021 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    10081 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    10141 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    10201 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    10261 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    10321 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    10381 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    10441 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    10501 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    10561 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    10621 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    10681 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    10741 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    10801 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    10861 attacaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    10921 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    10981 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    11041 ggcttcttct tcaagtgctt tagcacctga aatcgtgggg gaagaacatt ataaagtagc
    11101 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    11161 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    11221 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    11281 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    11341 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    11401 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    11461 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    11521 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    11581 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    11641 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    11701 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    11761 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    11821 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    11881 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    11941 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    12001 tggaacaccg tatatcagag gttgtaaaat cagcgttttg tcctgagtat tagagaaaca
    12061 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    12121 tgaacaccgt ttattt
//
LOCUS       DAZUKC010000073        11813 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000073 DAZUKC010000000
VERSION     DAZUKC010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.075"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..205)
                     /locus_tag="ACG80T_002066"
     CDS             complement(<1..205)
                     /locus_tag="ACG80T_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841700.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(221..835)
                     /locus_tag="ACG80T_002067"
     CDS             complement(221..835)
                     /locus_tag="ACG80T_002067"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFS4841701.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(982..1665)
                     /locus_tag="ACG80T_002068"
     CDS             complement(982..1665)
                     /locus_tag="ACG80T_002068"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFS4841702.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1774..2232)
                     /locus_tag="ACG80T_002069"
     CDS             complement(1774..2232)
                     /locus_tag="ACG80T_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFS4841703.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2316..4079)
                     /locus_tag="ACG80T_002070"
     CDS             complement(2316..4079)
                     /locus_tag="ACG80T_002070"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFS4841704.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4300..5760)
                     /locus_tag="ACG80T_002071"
     CDS             complement(4300..5760)
                     /locus_tag="ACG80T_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841705.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5968..6894)
                     /gene="glsA"
                     /locus_tag="ACG80T_002072"
     CDS             complement(5968..6894)
                     /gene="glsA"
                     /locus_tag="ACG80T_002072"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFS4841706.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6917..8344)
                     /locus_tag="ACG80T_002073"
     CDS             complement(6917..8344)
                     /locus_tag="ACG80T_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4841707.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8375..9589)
                     /locus_tag="ACG80T_002074"
     CDS             complement(8375..9589)
                     /locus_tag="ACG80T_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFS4841708.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(9675..9902)
                     /locus_tag="ACG80T_002075"
     CDS             complement(9675..9902)
                     /locus_tag="ACG80T_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841709.1"
                     /translation="MKNKFENRSDTRCGSFLYILYEVFFIPYEKISLKIKKDENKRFS
                     IKRRPFIFCLYLCEKRKRAQHIKLLLIVCLL"
     gene            complement(9930..10172)
                     /locus_tag="ACG80T_002076"
     CDS             complement(9930..10172)
                     /locus_tag="ACG80T_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS4841710.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(10204..10761)
                     /locus_tag="ACG80T_002077"
     CDS             complement(10204..10761)
                     /locus_tag="ACG80T_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS4841711.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(10774..10962)
                     /locus_tag="ACG80T_002078"
     CDS             complement(10774..10962)
                     /locus_tag="ACG80T_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFS4841712.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(10975..11541)
                     /gene="amaP"
                     /locus_tag="ACG80T_002079"
     CDS             complement(10975..11541)
                     /gene="amaP"
                     /locus_tag="ACG80T_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFS4841713.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         3465 a         2447 c         1933 g         3968 t
ORIGIN      
        1 atttgttaaa gtaattaaac aatccgctaa tcgtttgatc tggcatttct atttccttac
       61 catctaaagt aacagtagta acgacaaaat ggtcttgcaa tggcggaact tctaagtact
      121 gcccttgcag aaaatcattc actgtttcat attctttttt catttctttt gaatctgtcg
      181 atggtttata gcttattttt aacataacta gtgctccttt ttattcggga cgtgcaatcg
      241 tgaaaatttc tcctattgct gcataatcat ttcctgccaa acggcttaca gcattcaatt
      301 ttcttggatc gatccttcct gtcttatcat catagactgt ttcatttaaa tgatatttct
      361 gtacttctag caaaaataaa tcagctgtta ctttattttt attccgtatc tcgacatgtt
      421 catataagga tgtttcaaaa cgaacttttg cttctgtcaa tgctggtact gaaatgatta
      481 ctgaatcagt taaagtcaag tcagtcaggt acagttcgct ttcctcatta ggcagattgg
      541 ctgctgtttt atttacttct tctacgttgt cctcatcaag gatatggata acagcttctc
      601 ttgtctcaag aatattcttt gctgtgtctt ttactgcatc ttgttttctc tggatcgcca
      661 aggataagat gggtcgattc gaactgacga tattgaagta actgaagggt gcgatattca
      721 ctatatcttt tgctgattta gtcgttacta ctgctactgg tctagggatg actgaaccaa
      781 tcagcaactt gtaattttca cgttctgtca attctgaagg actccgttca aacatggttt
      841 cgccttcttt ctataaaatt cgttaaaaag cagatcttga agacaaaatg aaaagtaatg
      901 cttttttcat cttcttagaa tctgctttaa actatctatc aatttttctt ttttgtcaag
      961 aatctgtgcc gtgcgtgttt tttagttatt tgctttactt gcggcttcga catatctttt
     1021 agtgatcaat tgcggacggg tgataataga tccaactacg actgaaaaag cacctaattc
     1081 atatgctttc tctacttgtt caggagagtc agtatgtcct tccataatta ctgggatatc
     1141 gacttgttct atgatttgat tcaataaatc aaaatcaggt gtctgcgtct ttcgtgaata
     1201 ttctgtatag cctgctaaag tcgtagacaa aagatcaaaa ccatcttctt gcgcttgaag
     1261 cgcttcctca aaagtcgaaa tatctgccat agctaaccga ccgtgttcgt gaatcatttg
     1321 gacaagttca gcagttgttt tttctgtcgg acgtttacga gaagttgcat ccagtgcaat
     1381 gatttctgct tgcgtctcca aaagttcttc cacttctttt tctgtcgcag taataaacac
     1441 ttctgaccca tcgtaatctc ttttgataat ccctattacc ggtaaatcaa cgattcgctt
     1501 gatttcattg atatcttctg tcgtatttgc tcgaatacca actgctccgc cttcttttgc
     1561 agccaaagcc attctcccca taataaaaga actgtgtagc ggctcgtttt ccaaagcttg
     1621 acaagaaacg acgactccgc ctttgatttg ttccagtgta tccacaatat ctcctccagt
     1681 aatgttatat aaaaatacag cttcatataa aaatgtaacc cttttctatt caggagacaa
     1741 taatgagcta tttctttcag cagacctttc ttactaaaag aaaattactt caaattcttc
     1801 gtatacgacc agcttctctt ctgtaaattc tatgttcaac gcttctttat aagcatgttt
     1861 aaaaaactca gcatggactt tccgccaata agctaatgtc cgatcccctt ctccttcttt
     1921 ccaagcatgc tctttcgtga cttcactaaa aggcaagatg gtcacattta tatttttgat
     1981 gatacaaact ggttcatttt ttccattcag gaccacgtct aattgaccaa cttttggtaa
     2041 aggctcattc tcttgtttat acagatcgta accacttgat gtaccagtct ttatcttttt
     2101 tacaactagt tcgctcaact cgtctgccat tttttctgta tccccaaatg aaaagaaacc
     2161 taatttttct gattgataac taggatattt ttcttggaac tttttgacat attcttctac
     2221 ttgctgatac ataacatcca cccctttgct ttttataatg aaaaaaccat ccagatacta
     2281 gtataaacaa ttatctggat ggtgagcata atgaattatt gaatattcaa gacacgcaga
     2341 ctatcttcaa cgatttgttt tggtgttaaa tgataacgtt cataaagctg ccctacagga
     2401 acgctatccg tgaattcttt ctctgcacca aaatttaata cgttcattga agaatcacca
     2461 taataacgac tgatattttc gccaaatcct cctgacaaac taccatcttc taatgtgatg
     2521 actaattcgt gtgtttcttt taatttatct aatgtttcta cgtcattttc tgtgatcgtt
     2581 cctggattga tcaacgttgc ttcgattcct ttttttctaa aagcttttac tgtttctttt
     2641 cctaattcaa gaaaaccgcc taaagctaaa attgctactt ttcctccatt atttactgtt
     2701 tgataggctg gaatactata atcatttgcg atagctgtac catgttctac accatgtgta
     2761 ggaattcgga ttgctacagg ctgcgctgtt tgttcgaacg cccaatctag catagagatc
     2821 atatcctctt ttgttgctgg agcaagatat ttgatgttcg ggatacttga taagtaagga
     2881 atgtcaaatg ttccttggtg tgttcggtca ctagctgaga tcgttccgcc accgactaaa
     2941 ataatcactg gattctcgtt gatcgctaaa tcatgtgata attgatcata tgcccgttgc
     3001 aagaaagtac ttgattggaa gacgactgga cgtactccct gagaagccaa agcagaagcg
     3061 aaagtgatag agtgctgttc agcgatcccc acgtcaaaat aatgatctgg atgtttttct
     3121 tggaatgttt tcagtccaaa ggcgcctggt atcgctgcat tgactgcggc aataggagct
     3181 gtacttgttt gcattttctt atccaaataa tccatgatga tcatattaaa tgtttctcca
     3241 gaagttactt gctttgcctc cccggtggac agatcaaatg gtacatgcca atgatatttc
     3301 attttattat cgattgccgg ctgataaccg cgaccttttt ccgtatggac gtgaagtaca
     3361 atcggatgat caatatcttt tacttcttca aataaacgga tcaccgtttc cagatcgttt
     3421 ccattatcca cgtaacgata atcaaatccc attgctttaa ataggttatt gctgctttta
     3481 ccgttggttt gacgaagctc agctaagttt ttatataaac tgccatgatt ctcatcaata
     3541 gacatctcat tatcgtttac taatacgatt agattggatt gtaaaacacc accattgttc
     3601 aatccttcaa aagctaagcc accacttagg gagccgtcac cgataaatgc taccacattt
     3661 cctgattctt tcttcagatc tctcgcttta gctaaacctg ttgctaagct gatagatgta
     3721 gatgtgtgcc ccactgtaaa atagtcatgt ttactttcat gttggttgga atatccgctc
     3781 acttcacggt aatgtccttt ttcaaaacca ttttttcgac cagttaaaag cttatgacca
     3841 taagactgat gggaaacatc ccaaacgatt ttatcctttg gtgaatcaaa tacataatga
     3901 aacgcaatcg ttaattctac tacacctaga ttcggaccaa cgtgcccacc tacatcgctg
     3961 tccttttgca agattttttg gcgcatctct tctgccaact gtttcatttc ctcaacagat
     4021 aattttttga catctgcggg gctattgatt gcatccaata catgtaatac tgtactcata
     4081 atacattctc tcctccgtac tatcttcttt taatgaaaat agatttgtta cactttatct
     4141 attctactac ttagagttga ctctaagtca atagcatttt aaaaaaattt cagagaaagc
     4201 ttgttatata agtagatctt cgcacgaatc aggactcaaa taaaaacctt ctacagacaa
     4261 ttaaaaagct ttgtagaagg tctcctaatt aaggtctcat caagaaatat gtttttttaa
     4321 taggtgaagt tcctgagcaa tatgatacaa atggatgttt gcacctttta atgagattgg
     4381 atagatcttt gtcttttcag tttctaatcc attttgatag ctagcagcat cgcacaaaac
     4441 cactcttttt ttcgcacata gacattcatc aggcaattct tctaatgaac gaaataaaat
     4501 aggatttatt tcagcgatgc cttcttccgc atacaaatga aattgggttc gataaaaaaa
     4561 ttgtttgact gaactttctg aaacaacaaa aatttctatt tcctgtctcc ccgcttgttc
     4621 actgttgatc aaagaaagac ttttgacgaa ttttcgaatg aaattttcat tccatcttag
     4681 ttggatttga tttttctttt tccactcttc caatatttct acaatttttc cgtacagaag
     4741 ttgttctttt tctgatagaa gatagatcct ttccggtaaa aatacttgtc catctgccca
     4801 caacgtacgg acaaattgta aaaaggattg cttgaaaata aaattttctg catcgtcgat
     4861 ttcagcgata ctaataatct gtaaaatcaa ttcattcagt gataggtcat tgagaacaaa
     4921 atttttataa acaacttcga aatcttttct taacagctcc atgttctcat tggtataatt
     4981 tctactgttg aacaaagaaa aaatataata gacatcttct tcttgtaagt ctagaattcc
     5041 tttttgctct tccaatcctt gacagactaa ttgaaataac ggcttcgttt ctgcgctttt
     5101 cttatcttct tctttcagtc caaggggaaa tgcctttctt ccgattgcga tctctattcc
     5161 tcgtatgata aaggactttt catcttccga atatcttctt ttaaaaaaat gagtctctac
     5221 ataatcaatc aactcgttac aagcttcttc tacatacgga tttttatttt catagccttc
     5281 ccaattgaca taacgtacta aagctagcaa gatgttccgc gagtcaaatt ctgggataat
     5341 gatttcttta tttttcaaat aatcattttc tttaaaaaaa tccaatacga tttttttgat
     5401 tgtatatact ttgctcaaag aaataaattc aagatcagcc agttctgtac tcgtgaatat
     5461 accattaaaa taataactta aaaactttaa aaaataagat tgaccgtaca gaaactgtgt
     5521 gagctcagaa agagcaatac catgtttgaa tacgagttta tacttatgct tctctcttac
     5581 ttgttctttt atacaatatt ctagacactc ggctagactt tctagatcgt gaaggatcgt
     5641 tggtgtagtc acccttagag aaatagctgt ttgttcgatg tctatttctt ggaattcaaa
     5701 taattgttca cacagataca cttttctaaa tatatttcgc tcgatgtaat tttccagcat
     5761 tcctgataca cgcctccagt ttttcttatg attttattta atcatatctg taaatcgtat
     5821 ttttgtttcc tttttattta gaaaagccct ttcaaaaacc aataaaaaaa gaaactggga
     5881 caaaagtcaa acaagctcac aatttcagcc tcattgtagg tagctttgag ccgtttggac
     5941 ttttacccca gcctctttca agaatggtta ttcaaagatg tcagcgtcta gttctttgac
     6001 tacatctttc aacaattgga taccagctgc gctgttacca aatggatcta gtgccgggct
     6061 gaagacacct atgccgtaac gatttggcac agctgccatc aacccgccgc ctacaccgct
     6121 ttttgcaggt acaccaacat ggacggaaaa ctctcctgat tcatcataca aaccagcagt
     6181 tgtcataatt gatttgacga ttgtagcact ttcttcagta atgatttgtt tgccattcca
     6241 aggagcaatt cctttatttg ccagcacagc agctaatttt gctatgcctg tagcggtgac
     6301 attgagggag cattgtctga aataagcatc gagtatctcc ggtacgtctc cttcgatcat
     6361 ttgattacct ttcatatagt aagcaagaga gcgattgata tcacctgttc ttgattcaga
     6421 atgatagatt tcttcattca atgtgatatt aggatcatca cagatctctt tcataaatga
     6481 caagattcgc tcaaattttt catctgcatc tttaccggca atcaacgagg ttgttgcaat
     6541 cgctcctgca ttaacaaaag gattcattgg atgtttgcga tgattgattt ccatattcat
     6601 gatagaattg aaggcaaaac cggttggttc cgtgttgatt gtttgaaaga ctttttctat
     6661 tccgttgtct ttgattgcta ataacaatac gggaactttt gacatgcttt cgatggcaaa
     6721 gcgcacatcg gcgtctcctc cctcaatcag ttgttttgta tccaaatcat aaatcgccat
     6781 acctaattgt ttgggatcca cttcagctaa tgctggaatg taatccgcca cttgtcctgt
     6841 ttgataatag tggaaatttt ttccaatcaa ttgttctaat tcttttactt ccatttgaat
     6901 aaccagcttt cattttttat ttttctgcat gctggaattt atgacggcat gcgtagatcg
     6961 taaatggaat aactaagacg ataagataag aaacaatcag aatcgtttca taaatcccat
     7021 gctgtccagc tgcgatacta gcaggtggaa caaatgagat acaaaaagca aaaagtgaag
     7081 tcaaaagacc taaacaagca acgattactt ttacaacttt tccgcctgga acctgatagg
     7141 ctctttcaag actgtcatct ttaaaaacca gcttcaaata gcccgcgaaa aacataaagt
     7201 acaacatcag gtagattaca acagtcaatg acatcgctgc aagaaatgaa agattattcc
     7261 ccccgccgct taatgtaaag acggctgccc agattgttgc aacgatccca tttgcgatca
     7321 tcaaatgagc aggaacaccg tgtgtgtttt cttttgcaaa atattcagga agtaatcctt
     7381 cttctgctgc tacgtgaagt gcttttgaag gagaggttac ccaagaagca atttctccga
     7441 tgacacctaa acaaacaaga atcgcaagaa ttttcgcaat ccatgcaaga tttggatcaa
     7501 tgtgtcttaa taggaattct aacgattgaa tcacccctgt attaagagac aaaccatgtt
     7561 gaggtactgt agtagcgacg gtcaatccgc caaaagtatc taaaatgatt gccagaataa
     7621 ccaatacaaa cattgctaat ggataatcct ttttagggtt ttccatttca tttgcatgac
     7681 tggcagatgt ttctacgccc atatatgcta aaataaagga aacaaaaaca accagcgtag
     7741 gcaatttcga aaaatcagga atcaatgttt tcatagacag tatcgtttgt agtggagcgc
     7801 ctgaagcgac ataatggatc cctaagccaa acaagactaa tgctggcaac aagataccag
     7861 ctacaaaacc gatccgagca atcaatgaag tgtatttggt tccttttaac tgtgagagta
     7921 aaatagccca gaaaataatc aataggattg ccaatttcag atatttattg ttttgtatag
     7981 caccaatacc cgttgcatag gataatgcac cagttaagaa atacagcatg gtcaagtagc
     8041 caactgttat ctcaaaccat tggaaaaaga ttgcggcaaa accgaatcgt tttcccaatg
     8101 tgcgactgac ccaagtatag acaccgcctt tttcccatcc tttcaccgtt gccatctctg
     8161 ctgcgcacaa tgccacaggg ataaaccata gcaaaccgcc taataacaag aaaaatacta
     8221 agctgaagcc tgaggtcgcg aatgtgggat attcatataa tgaaacgacc attgagatgg
     8281 tcatagccaa aaagccaaag aacgttaatt tttttgcagg aattttcgtt ttgctttgtt
     8341 gcatgaatct tcctcttttc tattttaatt tgtttcaatt gagtttgttg ccaaagaaga
     8401 aaaagtccaa tccttcttct tgttcttctt ttgtagtagt gatcgttgta aagtgcatcg
     8461 ccgattttaa aatcactgtt gtcagcacat aggcatacac ggcagtaatg atgacaccaa
     8521 tcacttgaat ccctaagaag taccagctgc ttggattcgt tagtaaatgg ctgttagatg
     8581 caaagatccc tatcagaatc gtacctgtga atccgcccat gccatgaaca ccccacacat
     8641 caagtgcatc atcccattgt ttctttttac gaaaatcaac agcagcatga cagacgatac
     8701 ccgcaatgat cccaatcagc atggcacttt ttgcttctac ataaccggca caaggcgtaa
     8761 tggctgccaa tccagcaacg gatcctgtta gtacgtcgac aaaatcaaag ccatttcctt
     8821 ttacttttgc aatgatcagc caagtaatca tcgcaaatgc aagagcgatg aatgttgaac
     8881 caaatgcatt gactgcttgt tcatctgctc gaagtgcacc accggaatta aaaccgaacc
     8941 agccaaacca aagcagtcct gtaccgatag cagctgccat aagactgcta tgctgacttc
     9001 tttctcctaa aggaatcttt ctttgaccaa gtgtgaggat acatgctaaa gagccgaatc
     9061 cagctgtcgt gtggatgact gctccaccag cgaaatctct aaatcccatc tgatccaaaa
     9121 atccgccacc ccatatccag tggcaaacag ggaaataaat cagcagattc caaaaaataa
     9181 caagtataat atagcctttt aaattcaatc tccctgcaaa agctcctgtc atcaaaggaa
     9241 cggtaatcac gataaacatc agttggtaaa gaaagaacat taaaaatgga atcgttgcac
     9301 catgaaacat gttggggaag cttgtaacat gtctcatcaa aaagaaatct gccgggtttc
     9361 caataacacc gccgatatcc cgtccaaagg ccaatccgaa tccaccaaat acccacataa
     9421 tcgttgttat cccaacggag agaaaagatt gagacatgat cgttagtata ttattcttcc
     9481 gtgctaatcc gccgtaaaaa aaagctagac caggagtcat caggcaaacc attgctgtac
     9541 ataaaatcat gaatgctgta tctcctgtat tgataggtaa tgtattcatt tttttcgcct
     9601 cctttctacg attacgagta taaaagaagg tgtagttgtt tttttcttct gtttttttga
     9661 attaatactt gcatttataa aagacagaca attaataaaa gcttgatgtg ttgtgctctt
     9721 tttcgttttt cacacaaata aagacaaaaa ataaacggtc ttctttttat agaaaaccgt
     9781 ttattttcgt ctttttttat ttttaaagat attttttcat aaggaataaa gaatacttca
     9841 tataaaatat ataaaaaaga accgcagcga gtgtcgctac ggttctcaaa tttgttcttc
     9901 atttcaacat aagtttttct tcaatttgct tataatttac gtgcaaagaa tgaaacgact
     9961 aatactaaaa tgattgcacc gatcaatgat ggaatgatag ccattcctgc aattgttggt
    10021 ccccaagttc ctagtaaagc ttgtccaatt gctgaaccta ctaaaccagc aatgatgttt
    10081 gcgatgatcc ccattgatga tcctttattc gtgattgctc ctgcaattgc tccaatgata
    10141 ccgccgacga ttaatgccca aataaatccc attttaaatt cctcctaact gacttcttac
    10201 ttcttattgt acacgtggtt cgttattttc tttgactttt tgtgcacctt tgcctacagc
    10261 agattttgct ttatctgttt ggcgtgaagc aaattgtcct gtggattttg ctgcttctgt
    10321 tactttgtct tgtactgttt cttgatcttt ttggtattct tcttgtgttt tgatgtctgc
    10381 aacattcaca ttcacttcga taacatctaa atgtgtcata ttgtttactt cagtactgat
    10441 caattctttg atttggttgt aaatatcttc gatgtcttta ccgtattcta cgatcacatc
    10501 tagatccaca gctacttgtt tcttacctac ttctgtattg atacctgctg ttacgttatc
    10561 tgtgttgact aatttttcag ccatatttga gaaaaatccg ccatctaatg tcaatagacc
    10621 atctacatct ttcaatgcat aaccgatgat tttttggatg actttgtctt cataagtcaa
    10681 ttcaccattc atcatgcggt tttctccttt ttcatttgcc aaaactgatc catttgttgt
    10741 attcatgttt ttgttttcca tctttgtttc ctcctatgat ttaaaaaatt gatcaactat
    10801 tcctgactgt ttcaaataaa aaccgattcc tgcgccaatt gctgtcatga ttatgatcaa
    10861 caatgtcttg aaaaatccaa tcgttaacca taaaacagct agcagtagtc caagaccccc
    10921 aaagataata gggagcttgt atgctgaaaa tagttctttc attatttggc cctcctattc
    10981 cactcgagag tgcttgtagt ctcttgtatc ttcttgttct tcataaccgc tgtatgtgac
    11041 tgccactttg accgtttctt ttgttccgag tactttagtt acttcttctt cgatatcttg
    11101 catcaataca cctgttttcc ctactaatga agaagttcgt ttcaagtctc cgttgacatg
    11161 gatatggatt cgatttttcg ttgaacgtat ccgtacttta ggtgaatgaa tgaactcttc
    11221 ttcgtgtaaa tgggaacgta ccaacccttc gatcgctttt ttgtcgattg ttagtttgcc
    11281 atcttcccgt ttcataacga aagttcctct tgactttgga tagaaaagaa cgactagcaa
    11341 gatgataata gcaagaacgg caaatactac agctgcccag aaaatatatt gttgcatgta
    11401 gtaattcgtt aaagtaaaga agcgaaatcg ttccaattgt tcaggcataa tatacggcgc
    11461 attgatcaac gcaatcgcta gaaatactga aagtagcagt aaactcatga taacaccgat
    11521 tatttttaac cccttattca ttttccacct cctataaact tatttttacc ctgtaaataa
    11581 ctctaccgac aatcacagta tagatagcga acagttagca tagcatcagt aaataaagcg
    11641 ctaccaaaca ttgctggatc ataacggcgt tttattgcca gtctgatact gaaataacaa
    11701 ttgcttactt attctctatt tacttttgat tgctggctat acttaaagga tattattcaa
    11761 gattaaaaat tgcaaagatt atgctcagtt attattacaa gattctattt att
//
LOCUS       DAZUKC010000074        11422 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000074 DAZUKC010000000
VERSION     DAZUKC010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.076"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            302..508
                     /locus_tag="ACG80T_002080"
     CDS             302..508
                     /locus_tag="ACG80T_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFS4841714.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(710..1711)
                     /locus_tag="ACG80T_002081"
     CDS             complement(710..1711)
                     /locus_tag="ACG80T_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4841715.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1716..3629)
                     /locus_tag="ACG80T_002082"
     CDS             complement(1716..3629)
                     /locus_tag="ACG80T_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS4841716.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3797..4303)
                     /locus_tag="ACG80T_002083"
     CDS             complement(3797..4303)
                     /locus_tag="ACG80T_002083"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFS4841717.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4466..4660
                     /locus_tag="ACG80T_002084"
     CDS             4466..4660
                     /locus_tag="ACG80T_002084"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841718.1"
                     /translation="MIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFIH
                     LLKLTNNGVTEMLSTHSISY"
     gene            4676..4903
                     /locus_tag="ACG80T_002085"
     CDS             4676..4903
                     /locus_tag="ACG80T_002085"
                     /inference="COORDINATES: protein motif:HMM:NF015893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HFS4841719.1"
                     /translation="MDELSLSELYIEIRTSPSLLKKPIIYDQSAKLLTGFSEDKIRMF
                     LSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(4929..6044)
                     /locus_tag="ACG80T_002086"
     CDS             complement(4929..6044)
                     /locus_tag="ACG80T_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841720.1"
                     /translation="MKNYHLSCSLKKGNPIEGEEYRIRYFFHLMYWQSFDENHSDILS
                     SIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSITYVPEEMNKFVHPL
                     ITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYLEEDLLDTPDKVQES
                     WGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGTKKKVDAFGKSAEDR
                     DFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCMLVRDIFIKHEQPVIF
                     YIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYPIDKKAYDQTNTQIF
                     YWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6089..6445)
                     /locus_tag="ACG80T_002087"
     CDS             complement(6089..6445)
                     /locus_tag="ACG80T_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841721.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6743..7636)
                     /gene="sgrA"
                     /locus_tag="ACG80T_002088"
     CDS             complement(6743..7636)
                     /gene="sgrA"
                     /locus_tag="ACG80T_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFS4841722.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTGKVNNELDLVPII
                     PTKEQKRNAGSNLKSVLKQPDFVSRGIDHQEKAKETDNRKSDLPETGSRTLNKLITWM
                     GVLLILIVGASYFRSLFHRVK"
     gene            complement(7832..8671)
                     /locus_tag="ACG80T_002089"
     CDS             complement(7832..8671)
                     /locus_tag="ACG80T_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFS4841723.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNRTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            8856..9747
                     /locus_tag="ACG80T_002090"
                     /pseudo
     CDS             8856..9747
                     /locus_tag="ACG80T_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
     gene            complement(10341..11036)
                     /locus_tag="ACG80T_002091"
     CDS             complement(10341..11036)
                     /locus_tag="ACG80T_002091"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFS4841724.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(11020..11418)
                     /locus_tag="ACG80T_002092"
     CDS             complement(11020..11418)
                     /locus_tag="ACG80T_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFS4841725.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3418 a         2266 c         1713 g         4025 t
ORIGIN      
        1 tcacagttga tatgattgtt atgtaaaata aaaaagttta ggatacatcg aatttttggg
       61 gaaattcttt tgggggtatc ctaaatcttt tctattatat tcccatattt tctattattg
      121 gtctagacgt atgatggtaa actttgtacc tatctgattg ctgaatcatt aattgcttat
      181 cattagccat ctttgtttca agttcgctag atgatcattt tattcacatc ctaacaaact
      241 tgtcacattt atagattctt aatctattta tcatacaatt ataaaaaagg aggtgctgct
      301 tatgaaagct atccatattt ctttgctttt tttcgctagt gcattgattt tatttgctat
      361 tcatctttgt tcccagcctt atatcaataa caacggaata ttagtggaac cagcattctt
      421 ttgcttacca ctcggctttt tgtcccttct tgcctccgca ggcttcggat ttgttgcttt
      481 ttttaaacga tcaaaacaac aaatctgact tataaaattt acgtaaaaga gatcgtgaca
      541 ataatcgtgt gatattccca aatttcgaat aaaaggtgcc ccttgccatc tctaactcct
      601 cagtcacaag ttacaatatt cataaaccat gaaaagcttg tttattgaaa ttgctccttg
      661 tgacttggag ctaagtgaca gattacaacc tcttttgcat cataagactt tactccttga
      721 aaaacattac taacttttga tatgtttctt gcgcttgttt attgctgaaa tcaaactgat
      781 attcatggtt caaaacagca tttttctccg cataatctac ttttgtgact ttcacccccg
      841 ctttctcaaa tacagaagtt acttcttcca tttgtttcgt aaacgtattt gtatttccat
      901 ccgttaagaa tgtgttttgc gtccaacgat ccgcatgtct gaccaaggct aaacttttga
      961 ccttctctga ttgttcaaag tccttattat caaagtaagc ccatccacat ttttcataaa
     1021 gaaagctaga gattttatca cttacttgag taagtgcaga aggatccaac agaccagaca
     1081 aagaaataaa cttcgtaatc tgcttgttct ccaaagtcgg atcaagatga tcagcttttt
     1141 taatttcttc attttgaagt gctaacaaat attctccact aatttgtgca cctgccgaat
     1201 ttccaccaat cgaaatattc gaccaatctg cttgatcttt tatctctttt ttagctgctt
     1261 gaatggcttc gttcaattgg ataactggtg taggatgttt tgcttccggt gatcgttcat
     1321 aatctatatt tacgatgatt tgctgcgtgt ctgaacatag atattccaaa taatcacgac
     1381 aatcattttt atcccctcct acatatgcgc ctccatgcat ccagaaaaga atcggtaatt
     1441 ttttcttttc cactttcggt gtgtagatat ccatattatt atttttgtac tttgaaggat
     1501 aggatatttc tttttttata tcgatttgtt cgattttttg ctggaaatca tctggtcggg
     1561 catatacttg ttctttttca cttgtgtcaa attgttttct aacaagcaag ctaatcggct
     1621 ttggtgtgat ttgattaatc aaaaatacag tgactaaaat aactacgatg ataactggta
     1681 ctgcaatcac tattcgcttg aaccatttca ttcatttacc ctctctttct tcgccaaatc
     1741 catgtgataa tcgtgctaac agataaaaat ccacctgaat aaatgagaag cgaatgtttc
     1801 ttttctccag ttttcggcaa cattcttttt tttgcatctg tctttttttg tactttcact
     1861 gtcatctgaa tcggttgcgt atcatcactt gattgatcct ctcgaggttc attctttccc
     1921 ttattgtctg cattctctgt cggatcttgc ttttttggtg gagctactgg gatttttttg
     1981 taatagaaaa taatagttgg tatcgtctca ttaaaaatcc cttcagtcgg tccttcaaca
     2041 tttgctaatt cgtatccttc gaatgtcttt tgctctgtca tataattttc tccataagct
     2101 ccgtaaagct ggatttcgtc ctggtctttg agtagacttt tattgttttc atccacatac
     2161 tttacgttta tctgatcatg gaacactact ttcaaaaagt ccgttacatg tttcgtgtag
     2221 gcagcatggt caacttcctg tgcctttcca tgccctgctc cttctactaa taaaagatct
     2281 ttataccctc ctgataactc atataatttt tgcgcgttag acacaggtac tacatcatct
     2341 gctgttccat ggataatcaa tttaggcaat gaatctgaca ttccaatcgt aggtagagca
     2401 gcttttgtat ccaaatgcat ctcctgatca aaatagtatt gtttcaaaag gcccataaat
     2461 aaatcttcat gctcttgatc cccaatgatg tctaactgat tttttatggc atccggtagt
     2521 gcgggggcta ctgtctggct gtacgtaaaa cgtagttgtt catcaattga tgcaaatccg
     2581 caattttcaa taattccttt gacattgaca ggaatatctt tcgctaatac agacatcatc
     2641 gtagctgctc ccatcgaacc accataaagt aaaatctttt gagatggccg ctctcgaact
     2701 tcttgattta tccagtaaag gacgtcatct gactcatatt gtccataggt cacataatcg
     2761 cctccgctat taccagtcgc tctggaatca acaaataata aattataccc ggccttataa
     2821 aaatcattgt attcttctgt aacgatcccg ttattccaat tccctcgaaa tccgccatgg
     2881 atcatgactg ttttatctga accttgatcg acataatatg catatacatc catcattttg
     2941 ccatcttgtt tgtttagtac agtttgatga agttgttttg tatttggata gaagcctgtg
     3001 ttttctgtag gcgttgtccc atcatctgta cctccaagca tcgacatcaa cacactgttt
     3061 tttgcaaaag cgatatcgta caatagataa gtagcaatca gatgtttgat aatctcttta
     3121 tctacaccat aatcattaac agcaagctca gtgactaata actctattaa ttttgacttg
     3181 atgtcttctg ttggtccatt aagtatttct gtcatctgtt taccgaagta attcaaatat
     3241 tctttaccat tgaaattttc acctttaccg atatcatcat tttctgtccc attctctttg
     3301 tcacttactt tagcagaaga ggattcgtcg tttgacattt ttgtagctgt tgcttcttct
     3361 gtttgttggt tatctgtggt agcggtcgtt tcttgatctg tgctagtaac agatgtctca
     3421 accgttgttt cagtatttgt ttctgtttgt gaagtactcg atttcgtttc atttccagca
     3481 tcagtagttt ctgtggtttc tacctcccga gattccgatt cggaagaaat gctctctcca
     3541 tatactgtcc attgcgggac gcttgtactt acagcaaata agatgaccaa cgctcccttt
     3601 aatacttgtt ttgttctcaa gctattcact cctttttatg tttctaggag aattatatgt
     3661 aaacgcaaac attttttata caaaaaataa atggtttatc ccataaaaat agaacaggat
     3721 tattttctca ttttacgtaa taaagcagaa tggataaacc agtatccatt ctgctttttt
     3781 taaacgaaga aacaacttat tgctccaaaa atgtcagaaa tcgtttatcc caaattttct
     3841 cgtaaaaagc gattgtttcc tttgctttca taaaaggatt atcataagtt gtcgttgctc
     3901 ctctacacat cataagctgg tatcctaatc catgagcaaa cttcacagtc gtatctatac
     3961 aatactctgc ttgtgcccca cataattcga tcgaatgaca gtccaattca gttaataaaa
     4021 cactcaaatt agtcttataa aatgaattcg catgggtttt tctcacaaaa taactgtgct
     4081 caggaataga taattccgac aatagctccc atggttttgt ttgtggcact aattgagcat
     4141 cttcgtgttg aacgaaaata actggtttcc caatcgtttc gtatgttttg attcttttat
     4201 tcactaatgc tgtcactgtt tctaattcgt gtatcggcga agaatcaaaa catacccctt
     4261 tttgtaaatc gatcactact attgcgtcgg ctgtgtttga catacttgtg ccttctttct
     4321 tttttcttga tttaatagta tttcttatct agctagttcc attatacgtg acaacatttt
     4381 cttattttac acctctaaat ggcacttttc gactatttct tcatgttatt atttcataaa
     4441 tacttagcta ttggaggaat gtatgatgat taaattgtat acaggttctg gctgctcgtc
     4501 ttcaagacgc gcaaaacgtt ggcttatcgt ccatgatata ccgtttatcg aacagaactt
     4561 aattcgtcaa ccactaacca aaaatgaatt tatccactta ctaaaattga caaacaacgg
     4621 cgtgaccgaa atgctctcta ctcattctat tagttattaa aatcttgttc acagaataga
     4681 tgaactcagt ttgagtgaac tatatattga gattcgtaca tccccttctt tattgaaaaa
     4741 accgattatc tacgaccaat ctgcaaaact tttgactgga tttagcgaag ataaaatcag
     4801 gatgttcctt tctaaaacta aaagaaagca agagctcaac gaactcttgc tttctggtca
     4861 tcttgtcgat tttaaagaaa ataatctttt tgcaatgtct taattttggc ataaacaatc
     4921 tgcttttctt aattaacgaa atccaatttt tccaatatgg atctattttt ctcattcctg
     4981 atttttatga tttcagcttt cagacgcgcc caatcatttt ttgtaggttg tccattccag
     5041 taaaatatct gtgtattcgt ttgatcatat gcctttttgt cgataggata atcgctaatg
     5101 acaatatcag gtaactgatc agcggatgca ctatatttta ttgggcgttc agtcatagat
     5161 aatatccttt tctttgccca taattcctgt gttttaccat actttgattg aatatagaat
     5221 atgacaggtt gttcgtgttt tataaaaata tccctgacta acatacagta ttgaaaaatc
     5281 aaccgtctgt tcttttcata ataagtatta agctctggaa ttctgcgagc taccccacta
     5341 aaaaactgtt gcatgattgg atacatcact ggaaatatat gctgaaaatc tctgtcttct
     5401 gctgattttc caaaagcatc caccttcttt tttgtcccaa aaacccttga atacaaatgg
     5461 atcattgcca aattgacata tagataattt ttttctgaag ttgtaaaaca aatcttgaag
     5521 tttttttcaa aattttctac aatagacgat tcaatccagc cccaactttc ttgtacttta
     5581 tctggagtat ccaacaaatc ttcttccaaa taggtgttca ttacaccaaa cataaaataa
     5641 aggtaagctt gctctgttgg agtgatgtga ttcatcgacc agaagtttgc ttcaaaaaaa
     5701 ggctctagta tgtaatgcgt aaactcttct tttgttatca atggatgcac aaatttgttc
     5761 atctcttctg ggacatacgt aatcgagtgg ccttgtttca ttcgttgcaa cgaaattctt
     5821 agtaggctga tccatttctt tctatctata ctacgcgtat atggcgcatg tttcgcaaat
     5881 ttttctaata gttgacgttt attttttata cttgataata tatcagaatg attttcgtcg
     5941 aatgactgcc aatacatcaa gtgaaaaaag tatcggatac ggtattcttc tccttctatc
     6001 ggattacctt ttttcaagct gcaagataaa tgatagtttt tcaaaatagt attaagttct
     6061 cttaattttc tttgaatcgt acgcaaactt atataatttt ttgactaaga ttttctaagg
     6121 attgataagt acctttgtaa agtgaatcca gtaaaatata accaatcgtt ccctctaaaa
     6181 gtatagaata aactttactc agtaaaaact gagaagaaaa ctcgactttt atcatcttgt
     6241 tttctgaaag aaaagataat ttatattctg gatacctttc gcaaaacgat tctgcttgtt
     6301 gaatcaccga attcatctta gccagggtgt agtttagatc taaagcgata tccttcacca
     6361 catataattt ctcgtctctt tcataacaat agttcaaaaa ttgatatact tgcctttcat
     6421 tgatatcaat aaattcttca aacaaagtac atactccttt ttatctatta tttagactta
     6481 ttccgacagc tacatgaata acgtacaaat agattcgtta ctgatttttc agagatacta
     6541 ccatgcgcag tgtgcattgt caattattct tgctcttatt ttccatttgt gttcctgtta
     6601 tttcctgttt ctcaaatatg ttagtaaaca aaaatagatc tggcaaagct acaacttcgc
     6661 caggcaatac agcactctcg aatgaaatat tcactcaaaa atctatcgtt ttattcaatt
     6721 ttagatctgt tgcttttatt ctttatttca ctcgatggaa gagagaacga aaatagcttg
     6781 ctcctacaat caaaatgagt aacacaccca tccatgtaat tagcttgttc aaggttctac
     6841 taccagtttc tggtaaatcg ctttttctat tatctgtttc tttcgctttt tcttgatgat
     6901 caattccacg agaaacaaaa tcaggctgtt ttagcacact ttttaggttg ctccctgcat
     6961 ttctcttttg ttccttagtt ggtatgatag gtaccaaatc aagttcatta tttacttttc
     7021 ctgtgctgct ttcgctgctc gtgcttcctg tgctactttc actactcgta ctctccgtac
     7081 tgctttcgct gctcgtgttt tctgtgctac tttcactgct cgtactctct gtactgcttt
     7141 caccgctcgt gttttctgta ctgctttcac tacttgtgct atccgtactg cttggcggtc
     7201 caacaactgt ccaactgctt ggtttcaaat tctccgtgat cgtcatctgt ccatcataaa
     7261 aaacaatcaa atcaacttta tctttactct tgatctcatc aatcgatagt tctgcagaaa
     7321 tcattattgt atactcaata ccagcggtcc attgtacgtc ttttaaataa acttccccaa
     7381 taactttttc tgaacttcca ttaccatccc aaccatatgt tagaacagca tgtacatcac
     7441 tagcattcac gcttttgttt ggtttgactg gaatagtaat gacttgttgt gtcatgtcat
     7501 aaacaatccg atcagttttc acagtaaatg aaagattctc atttgcccgt tcatttgcag
     7561 aagtactgat tccaatcatt gctgcaatta tactcagccc aagaataaca aataatacgg
     7621 tcgctgtttt cttcatttct atcactctcc atataattac cttctatttg ataaaggaat
     7681 tatataaaat gaaaatgatg agaacacgac agtcagttgt gaaaatctaa caaaagatgt
     7741 cgtgtattcg cttgaatttg tttacttttt cgtttaatca atgacagact taaaaaagca
     7801 agaccgatat cagccttgct ttcttcatct tttattgata aacagcagtg tatttcttca
     7861 tccgctttgt tttatatgca tagagtaaca aaccaacaat caagactaac aagaataatg
     7921 tagttgatat agatgcttca gccccaaagt ttcccccagt aattattgta ttttgcgatt
     7981 ggatcagagt ggagaaaaaa ccttgttctt gcgttccgct gactggtata cccaaaatag
     8041 ctccttgtgt ccgattccaa gtaatatgag caccaatagc cgtaaataaa cttcctgtac
     8101 tgtagataag tgcgctgaaa aagcaacctg ctaaaaagat attgaaaact gccagaaatg
     8161 tcatgttagg attcaatccg tgcaaaagag caaataatgc agcagatcca atcattgcta
     8221 ttaacggacc aaatctaagc atctgatgct gaaaaaagcc acggaataaa agctcttctg
     8281 atgcccccac tgcaataaat aaaataaaac tcgttatcag cggcacaatg tttgcgtttc
     8341 ctgaatctaa ccgatacgcc cccgtcaaat ataatggact agtaataagc agaaacactc
     8401 caattccaac aacgagacct cccgctaaat acccccagtc ttttcctcga acagaaacac
     8461 cataatcttc tattcttttt ccttcgacct tactagcaag aaataaagct agtaaaaaaa
     8521 cagctaaatg tccgaccgta ccggcaattg ctccaatcca ttcactattt ccaataaaca
     8581 tatccgcaag tgcattattg acaagaattc ctaaaacaaa accaactaag actattaaaa
     8641 cagatccaat caaccgaata atcctgatca tctttcatcc ctcctttata ttaagtttaa
     8701 caaccctttt ctttcttgtc tcgtgaaaac tatgtaaatg ttctccatca tagagagaat
     8761 agatttttac gaactaagaa tatctacagc aaccttacga aaatgaaaga ctgtctccct
     8821 aattcttctg ttagactgtt tttaataagg aaaggatggg aaattttttg aaaaaacaac
     8881 tactaattat gtgcctttca gcatgtgcaa ttttactatt ctcaggttgt agcaagtcca
     8941 atgaaacgat tgcaacgttt aaaggtggaa cacttacagt tcaggatttt tatgataacc
     9001 caaaagttaa atcaaataat caaacactat taaaaaaaat gattgtcttc aaagcatttg
     9061 atgatgtata cggaaaagag atttcttcaa aggaagtaac aaaagaatat aatgaacaaa
     9121 tcaaaaaact cggtccaaac tacaaggaac aattaaaagc agttggccaa acagaagaga
     9181 catataagaa actctttaaa cagatgctgg ctttccaata cggtttgaaa gccaatgtaa
     9241 aattaacaga taaagatcta gacactgctt ggaaagaatt ctatccagaa gttagtacac
     9301 agatcattct attttcgact aaagaaggag cagataaagc aaaaaaagaa gcaaatgaag
     9361 gagaaaattt ctccaagctt gttcaagctt atggaaaaaa taaaacatta aaggaaactg
     9421 atggaaaaat gaattttgat tcaaccaatc cagagattca ttcctactga agtaaaaaaa
     9481 gcagcattta aactaaaaaa cggggaagtt tctgatatca ttcctgtaac agatccaacg
     9541 acttatcagc aaagctatta tctagtaaaa atggtaaaaa aacaggataa aggttctaat
     9601 aaagacaaat acaaatctga gctagaaaaa attgctaccg aagcaaaaac tcttgatacg
     9661 gaattcatgg ataaaacaat ccgtaaagtg atgaaaaaag acaatgtaac tattaaagat
     9721 ccttatgtaa aaaatatatt caaataaaca aatttgaccc aattggggat gtggcaaaat
     9781 ttgagaagtg atttttggaa attccttctt atttttgagg caaaccactt ttgccacaac
     9841 ttcatcgggg ccttttttta tttgtaccaa aacaaatcac tggatttgtt acaatcatga
     9901 ggatataatt taaaaaagtt ttttatttct atcacatgaa tcaatgcgct attaagtaga
     9961 ttctttcatg cttccctatt ttttcacttg ccgtatcgct tcaaaaacaa ctatcataaa
    10021 ctcaaactcg actgaaaagt tattttaaaa aagactaaaa atatcccttc tggacttgtt
    10081 cttcttattc tgttatacta gagaaacacg aaagatattt ttatgcgaca cttttcattt
    10141 gtttttatga aatttattca aaactggcgt aacttcagag ttacaaggat gtattagagg
    10201 ttagggaata catcgtttaa atgagaaata agtaatagtt agtaatagtc acccaaactt
    10261 taaattaatg aatattgaat cgtcagatag cctcctcgag ttttcgagaa ggctatcgtt
    10321 tattttatgg agaaattgat tcagaagtaa tcagcgacct tttttagttc accaatcgtt
    10381 tctgcttgga aagtttgcac ccaatcttct tggaagcccc tcactgcttt atcaatcgag
    10441 ggcatctcta atacttgatc tagtagctct ttacctaacg ttatcgcatg agctccagat
    10501 cgaatggctg tagtgacttg ttcacaattt ttgaaactag ctgctaatat ttttgttggt
    10561 ctttgcgtac gttcgatctc agtccgcaac gaatcaatca ctttcatagc atcaatgcct
    10621 tgattttgca tcctgttata ataaggtgca atataatctg ctccattatt aatagctaaa
    10681 tacccttgta attctgtgta aatagctgta ccggtaatca aataaccttc ttttttcaac
    10741 tttttgatcg cttgaaggcc tgcttgattc accggcacct tgataaacgt atcttttccc
    10801 agttcttcta tgataactcg agcatcctct atcatttctt cttccgttct tcctattgtc
    10861 tgaatatgga taggaatctg tccgattatt tttttgattt ctttcatatg cgagaagaag
    10921 tttgacgctc cttctttttt cagaattgta ggatttgttg ttacgcctgt taaattcaaa
    10981 aactgttgac aagaataaat actttttgta tttgctgtat caagcatgat ttccatatag
    11041 ctttccccca ttctgatcct cttgatcgat tttaatgatt gtcccctttc ttaaatgcgg
    11101 catttcctta tcttctacat acattgttcc tggaagttcc ggctcatgag atccatcaaa
    11161 ttttatcgtg atatgtccta attcattcaa attagtttgg acaacatctc ctactgctgt
    11221 gattacatac atttgtttat caaaacagag cttcatatca caagttattt cttttcttac
    11281 ctgattcaat tcaatcttat aacaataatc tgttaatgtt tccggtgctt catctccaaa
    11341 taaaatcatt attttttctt tcctgaatac ttctgcttct ataccaacat ctgttacaac
    11401 cgtttcaaag atcgtcatct ct
//
LOCUS       DAZUKC010000075        11152 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000075 DAZUKC010000000
VERSION     DAZUKC010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.077"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            114..1373
                     /gene="lysA"
                     /locus_tag="ACG80T_002093"
     CDS             114..1373
                     /gene="lysA"
                     /locus_tag="ACG80T_002093"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFS4841726.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1360..2160
                     /gene="dapF"
                     /locus_tag="ACG80T_002094"
     CDS             1360..2160
                     /gene="dapF"
                     /locus_tag="ACG80T_002094"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFS4841727.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2163..3044
                     /gene="dapA"
                     /locus_tag="ACG80T_002095"
     CDS             2163..3044
                     /gene="dapA"
                     /locus_tag="ACG80T_002095"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFS4841728.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3041..3757
                     /gene="dapB"
                     /locus_tag="ACG80T_002096"
     CDS             3041..3757
                     /gene="dapB"
                     /locus_tag="ACG80T_002096"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFS4841729.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3763..4458
                     /gene="dapD"
                     /locus_tag="ACG80T_002097"
     CDS             3763..4458
                     /gene="dapD"
                     /locus_tag="ACG80T_002097"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFS4841730.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4474..5475
                     /locus_tag="ACG80T_002098"
     CDS             4474..5475
                     /locus_tag="ACG80T_002098"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFS4841731.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5472..6833
                     /locus_tag="ACG80T_002099"
     CDS             5472..6833
                     /locus_tag="ACG80T_002099"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFS4841732.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6793..7944
                     /locus_tag="ACG80T_002100"
     CDS             6793..7944
                     /locus_tag="ACG80T_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFS4841733.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7919..9088
                     /locus_tag="ACG80T_002101"
     CDS             7919..9088
                     /locus_tag="ACG80T_002101"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFS4841734.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9263..10642
                     /locus_tag="ACG80T_002102"
     CDS             9263..10642
                     /locus_tag="ACG80T_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4841735.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10923..11152
                     /locus_tag="ACG80T_002103"
                     /pseudo
     CDS             10923..11152
                     /locus_tag="ACG80T_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3715 a         1948 c         2496 g         2993 t
ORIGIN      
        1 aatatactag acaattcaga aaattttgtt taatattatt ccaataaaat tcaaatcttt
       61 atcaaatgaa attccaataa aaacggaaaa aaagtgaaaa gaggaatgaa aaaatgacag
      121 aattgataat tggcggagtc acagcaagca cattggcaga aaagtataag acacctctgt
      181 atgtgtatga tgaagaaaag atgagacaaa cgatgctgtc attcaagcga ggctttgtat
      241 cgaaacgttt tgatacaaaa gtgctttacg cctctaaagc attccaaacg attgagatgc
      301 tgcatttagt tcgtgaatat ggattcgggc tagatgttgt cagtggtggg gaaatctata
      361 ccgctttgca agctgatttt cctagagata ctatctactt ccatggtaac aacaagacgc
      421 cagaggaact gatctatgca cttgaaaatg atttgttgca tattgttgcc gataatttga
      481 tggaagttga attattagca gaattaagcc aacgctattt aaaaaaaatc tgtatcatgc
      541 ttcggttgaa tgtcggagta gaagcacata ctcatgaata tatcgtcact gcgcatattg
      601 attcaaaatt cggcatgagc tatgaaagta aagagtgcca acaagccctt cagttgataa
      661 aagaaagcag ctatctagag ttagaaggat ttcatgctca tatcgggtca caaatttttg
      721 atgttactgc ttggttagct gaaatcgaca aattgacgaa ttacttaacc gattttgacc
      781 aacctctttc cttgaatctt ggcggagggt ttgggattcg ttatacacaa aatgatcagc
      841 cgatcccagt cgaagaggca ctaaaaaaac tgattgaata tacggaagaa gctttggaaa
      901 aacgaaaact gaccattcgt caattactga tcgaaccggg tagaagtatt gttggagaag
      961 ctggtacaac actatacacg attggtttca tcaaaaagac accacataaa aaatattact
     1021 ttgtagatgg cgggatgaca gataatatcc gtccagctct gtatcaggca gaatacgatt
     1081 gtgatttagc agcaaaactg tcggaagaaa aaacagagaa agtgacgatt gcaggaaaaa
     1141 tgtgtgaatc aggcgatgtg gtaattaaag aagcttattt gccgaaagca gaatcgggtg
     1201 atcttttagc tgtttattcc acgggagctt atggatactc gatgagcagc aactacaatc
     1261 gagcgacacg accagcagtt gtttttgtca ataatggcca atcaagactg attgttcgca
     1321 gacaagcatt ttctgatcta ctaagggggg aagtttccta tgatccttca taaatataat
     1381 ggctgcggaa atgattttat cttattagat tacgcagaag acacagatta tagtcaatta
     1441 gccgcttctt tatgcacaaa agaaaaatat gatatagacg gattgatcgc cgtaaaaatc
     1501 gatccgttgg aaatgatcta ttacaacaaa gatggcagcc gcgcgccgat gtgcggaaat
     1561 ggcattcgct gttttgctcg atacgtaaat gatcagaaca tgatgaatga acaggaattc
     1621 aacgtagaga cattagcagg cacaagaaaa attacggtgt tggatccctc acctttttat
     1681 tgcagcgtag atatgggagt gccagatgat tctccggaaa aaatcaaagc taatcaaact
     1741 tatccaataa aaaaccagga aatagagatc aatggtgaaa aagtaacgat cactagttta
     1801 tttttaggaa cgattcatac cgttgttttt gttgaaaatg cgcgtgagga agtctggaga
     1861 aaaagaggtg aactgatctg tcatcatcct ttgttcaaag aaaaaaccaa tgtcaacttt
     1921 gtagaagtag tcaatgaaca agaattgatc gtccgtacct atgaaagagg agtaggttgg
     1981 acacttgctt gcggtacagg ttgctgtgcc tcttatgtgg ttgctaagga aactgggaaa
     2041 atttcagcag atcaggtagt tgttcacttg gaacaaggag acttgatgat ctctggtgag
     2101 gatgcgatac aaatggctgg cccagcagtt catgaatgga caagaatatt ggaggaatga
     2161 agatgataaa aggttcgatc gtggcgctga tcacgccgat gaatgaagag ggatcagtag
     2221 attatgctgg tttggaaaaa ttgatccaat tccatttaga tgaacaaaca gatggattat
     2281 tagttttagg tacaacagga gaatcttcta cgctcacaca atctgaggag gagcagatcc
     2341 ttcaactgac agtaaagaaa gtggctggcc gtgtgccggt gattgcagga gctggaacga
     2401 ataatacgaa agaaacgatt gaaaaagcga agcattttgc ttctttgggg gcagacgcac
     2461 ttttggtcat caccccttac tacaacaaaa caagtgatgc aggactagct gctcatttta
     2521 cagcaattgc agaagcctca ccgattccgc ttattttgta taatgttcct tctagaacag
     2581 gcatgtcgat tccaatccat gtattagtaa atttagcaga acatcctaat atcattggtt
     2641 tgaaagaagc ctcaggagat atggcttatg tgatggatgc cgctcggttg attggagaag
     2701 aattcttcct ttacagtgga aatgatgatt tgatcctacc agtaatgagt gtaggaggaa
     2761 gcggtgtaat aagtgtatgg gcgaatatcc aacctaaaat agtccatgaa ttagtaaaag
     2821 atacgcagga cggaagatgg cagcaagcaa aagaaaagca attaaatgca cttgaactga
     2881 tccatgcctt gttcagtgag accaatccta ttcctgtcaa agcagccatg tcattattag
     2941 acttaccttc cggacctcta cgcctgcctt tagtttctct ttcaaaagag aaaaagaaac
     3001 aattagctca gcttttgtta aaagaaagga cggttgaaaa atgaaaatcg gactgattgg
     3061 cagtggacag atgggaaata gagtcgaaga agttgcacaa gaaagaggag atacggtcct
     3121 tcttttacgg acggcaccta aagaagcaac tacgcttgct tttacggaac tgccagaact
     3181 gcttattgat ttttctcatc cagataattt agagaaaatc ctttcgtata gcttatcata
     3241 tcaacttccg ttagtcattg gcacgacagg ttatacacaa tcacagttcc aagaaataaa
     3301 agaacatgcc aaacatgtac ctgtgatgta tagtgctaat ttttcgttcg gcatcatggt
     3361 gatgaatcaa ctaatcaagc aagcagctgc aatgttgcaa ggctggcaga tcgaactgat
     3421 tgaaaaacat catagtcgta aaaaagatgc accatctggt actgcaaata tgctgcttca
     3481 aacaatccaa gaagtgcaaa agctgcagcc cgtatacaac tggcaaggga aaaaaagaga
     3541 agaaaaccaa attggtgtac acagtattcg tgcaggttct ttacctggtg aacatgatgt
     3601 attgtttgct gctactgatg aaatatttac gatcaaacat gaatcttttt ctaatcgaat
     3661 ctttgctgca ggcgcagtag aagcagcgga ctggttgaaa gatcagtcac caggttatta
     3721 caaattagaa gatttattaa tggacaaagg ggtgtaaaaa acgtggaagc tcaagaaatc
     3781 atccgctaca ttcaaacggc atctaaaaag acgcctgtga aagtctatct gaatgtaacc
     3841 gagccaatcg aatttaaaga aagtaaagtt tttggtgaag gaagcagctt cgttgttttt
     3901 ggtgactatg agacgattgc tccggtttta gaagctgaat cagaaaaaat cattgaaatc
     3961 gaaatcgaaa ctgctgcaag atacagcgca gttcctttgc tagatattaa aaaaattaat
     4021 gctcgtatcg aaccaggtgc tattatcaga gatcaagtgt caatcggcaa caatgccgtg
     4081 atcatgatgg gagcgattat caatattggt gcagtaatcg gtgaaaacac gatgattgat
     4141 atgggtgctg tcttaggtgg acgagcgaca gtgggcaaaa actgccatat tggtgcagga
     4201 gctgtcttag caggtgtgat cgaaccagca tcggccaaac cggttatcgt agaagacggc
     4261 gtactggtag gtgcgaatgc agtgattgtt gaaggtgtcc atatcggaaa agatgcagtc
     4321 gtggcagcag gagcagtggt tttagaagat gtggcagcag aaactgttgt cggtggaata
     4381 cctgcacgtg tattaaaaat aagtgatgat cagacaaaag aaaatacagc attgattgct
     4441 gcgttacgcg aattatagag gagatggaga aaaatgcgcg aaatagatac ctgtattgtg
     4501 ggggcaaccg gattagtcgg ccaaacgatg ctcaaagtgt tagaagaata tgattttcca
     4561 gtaggacgat tgaaattatt agcttcaaaa cgttcagccg gtaaagtttg tgtattcaag
     4621 aaaaagaaac acactgtaca ggaattgaca gaaacttcat ttgaaggata tgatttagcg
     4681 ctattttctg caggtgcttc tgtatccaaa gagtttgcgc caatcgcacg aagtcaggga
     4741 ctatttgtca tagataattc ctcagcatgg cgggaagatt cggatattcc attaatcgtt
     4801 ccagaggtca atctggaaga ctatacattg aatcggctga ttgcaaatcc aaattgttca
     4861 accattcaag cggtacttcc gcttaaagct ttacaagaag cctttggtat ccagcgagtc
     4921 aattattcga cctatcaagc agtttctggt tccgggcaaa aaggcattga agatcttcat
     4981 cgtacgcaaa atgacgagga accagttttg tatccttata atattagcca aacggtgatt
     5041 ccggaaatcg atcagccttt ggaaaatggg tatacaaaag aagaacagaa aatgataaat
     5101 gagacccgta agattctgca tcagcctgct ttgccagtat cagcaacctg cgtacgtgtg
     5161 ccaatcgaaa atggacatgg agtctctgtg gcagttcaac tgcaaaaaga gtttacgacg
     5221 gaagaaatta gagagtgtct cagccagttt ccagggattc aattagaaga tgatctagca
     5281 gcacataaat atccgacgag cattcttgct agaggaacag atatggttta tgttgggagg
     5341 atcagaaaag acacttcttt agagaacgga ttgttgttct atacgactgc cgataacata
     5401 cgcaaaggag ccgctgcgaa tgccgtacag attgctgcag ctttaatgca gaaaatagcg
     5461 gaggtggcaa gatgagtaat acgctgaaag tagcgaaatt cggcggaaca tcgatgtctc
     5521 atgaagagca attcaaaaaa gtccgtacaa ttgttctttc agatcccaat cgccaagtga
     5581 tcgttgtttc tgctttaggc aaacgtacgt cagatgatga caaagtaaca gacttacttt
     5641 atttgattta tgcttacctt cagcatcatg ttgattggac acccctttgg aaaaggattt
     5701 gtgatcgttt cattcaagta agagatgttc ttgccctgca gttacctatt gaagatgaac
     5761 tagcacgcat acagaaacaa ctggaagacg aaaagataac tgaagattat ttagtaagta
     5821 gaggtgaata tctgactgct caactgatgg ctgagtatct tggttaccag tttattgacg
     5881 caaaagacct gattttcttt gactatgatg ggaaaatcga tcaggaaaaa acagcacagg
     5941 aaatcacaaa agcatttaaa agatatccga aaatggttgt tccagggttt tacggggcga
     6001 accctgcagg aaaagaaaaa ttattgggcc gtgggggaag cgacattacc ggcgcgatac
     6061 tggcgaagat gttgcaagcg gaaagttatg agaattggac ggatgtttca ggaatcatga
     6121 tggctgatcc gcgaatcatc gatcatccta aaatcatcga ggaaatctct tttagagaac
     6181 tgagagagat ggcttatatg ggggctaacg ttcttcatga agcagctgtt ttcccagttc
     6241 aagaggctga tatccccatc caaataaaaa ataccaatga tcctttcgct caagggacaa
     6301 aaatatcgaa tcacgaaata gaaaaggaaa atggactgac aggaatagct ggacgcaagg
     6361 attttctttc catcacttta ttcaaacgtc atatgtccga tgaaatcggg tttatctgga
     6421 aagcaatgag catttttgct aaacatgaga tatcaatcga acatattcct tcagggatcg
     6481 acaatatcgg agtgatcgtc tcagcagaag cgattgccga taagttattt ctcatcacga
     6541 aagaattaaa agaaacactt ggcgtggaag aaatcgtcgt gatcgaagac ttagctttga
     6601 tctctgtcgt tgggggtccg cataaagaat tgattggatt atctgggaaa gtgttatcga
     6661 tattgaacaa attagaaatt cgcacatcga tcttatcaca aggagcacag gagttgaatc
     6721 tgatcatcgg tgtaccgaat aatcaatatg aaacagtagt gaagggtatc tatgaaggga
     6781 tggtgaatac ggatgctgaa agaatatcga atggcactcc atcaaattcc tgaaatcggg
     6841 tttcatgaat tcaagacaaa aaaatattta tatgaacaag taaaaaacag cgaggccgtt
     6901 atccatgaaa tcggcgaaac aggactactc ctttattttg ataatcagca agaagaaacg
     6961 attgcttttc gaacagatat cgatgcactg ccagtcaaag aagaaacgaa tctctctttt
     7021 gcttctaagc atgaaggtta tatgcatgca tgtggtcatg acggacatat ggcgatgctt
     7081 ttagggttga ctgactttat aactaaacac attgaggatc ttatttataa tgtagtactg
     7141 atctatcagc catcagaaga aatttccggt ggcgcacaaa gcgttattca gtcaggattg
     7201 ttggataaat atgaagtaca agcaatattt ggctttcatc tatggcctgg actagctaag
     7261 ggagaagttt tctctcgacc aggacctttg atggcacaaa gcagtgaaac agacattatc
     7321 attaaaggcc agtctgcaca tattgcttct agtgaaaaag gaatcgatag tctggaggca
     7381 gctgtacgat ttttgaagaa tgtttatgat tttgaccggc aattaccggc agagtggaat
     7441 catctaatga aatttggcca aatccaaggt ggaacgatcc gaaatgtttt agctggagaa
     7501 gttattgtat ccggaagtat ccgttcttac agttcagata tgcaaaatta tttgaagagg
     7561 ggattagacc atttagcaga acagtttcac tctacagcta ctatttcttt tcgctataat
     7621 gacggctatc cagctgtaat caaccataga ggcttatatg cagcactgtt agattcaaac
     7681 aaactacatg aattagatgc tccagttctt caagccgaag attttggcat atatacgcaa
     7741 caatatccat gtgtattttt ctttttaggt gtcggtgata cccctgcttt acatgatccg
     7801 acgttcgatt ttgatatgga tgttttagaa aatggtgtag aatggtacaa aacgattctt
     7861 tttacagaaa atcttcttgt aaaaatcgat agcagttcaa aagaggagcg agtaaataat
     7921 gatgaattta tccaatcgat ttagtcctag gctttctaga atagaagtat caaaaatacg
     7981 acaattcgat cagcaaatat cgtcgatacc agatgtgatc aaactcaccc tgggagaacc
     8041 ggattttcca actcctgaac atgtcaagca agcaggaata gcagcgattg aagaagactt
     8101 ttctcactat accggaatga gaggattaga ggaactgcgg gaagcggctt gtatatttca
     8161 gcaacagcgt tatggattaa cttatgatcc gcaaacggaa gttttgacga cagttggtgc
     8221 aacggaagcg attgcctctg cgttgttatc tgttttggaa gaaggagaca aggttcttat
     8281 tccagcgcct gcttattcag gatatcagcc attagtggaa ctagctggtg ctgaattgat
     8341 cccgatagat acttctgaca cagggtttgt ctgtcagccg gaacaatttg aacgagcatt
     8401 tgaacaatat ggatcagctg tcaaagcagt cattttaaac tatcctaaca atcctactgg
     8461 gacgacattg tctgcaaaac aattgaaagc aatcgctgag gttttaaaaa aatatccggt
     8521 atttgttata agtgatgaag tatatgctga attgacttat agtggaacgc atatgtccat
     8581 agctacttat cttccagaac aaacaatcgt gataagcggg ttatccaagt cacatgcgat
     8641 gactggttgg cgagtgggct ttatttttgc tcaaaaagcc cttattgacg aactaattaa
     8701 agtccatcaa tatctggtga ctgccgctac aacgatgtcg caaaaagctg cggctgaagc
     8761 gctaataaat ggggtgaaag atagcgagaa tatgaaagag caatatcaaa cacgaagaga
     8821 ctatctggtc cagcaattgg cgcctttagg atttgaagtg acccagccaa acggtgcatt
     8881 ttacttgttt tgcaaactcc cagctgccat tcaaactgac tcttggcagt tttgtgtaga
     8941 attagcggaa caggcaaaag ttgcgtgtat cccaggaatt gcatttggcc cagaaggaga
     9001 aggttatatc cggatttctt atgctagcag catggaaaaa ttacatgagg cctgcaaacg
     9061 gatcaaagac tttttaacag aacagtgatt catagatttt atcaaaaaaa caatcgttaa
     9121 ttttgcggta tattctgcaa ggagacgatt gttttttaaa tttagttcaa atgaacgcgt
     9181 ttcttttgat tcataactaa tgtaatgtta agtttaaatt gtttagaatg attttaaaag
     9241 aatattcaag gaggtttcat caatggaaaa aatcaaagtt gtcattgtag gtgcatctca
     9301 tggagggcat caatcgatct tggaactatt gacacgatac ggtgaaaatg tagatatcac
     9361 gctatttgaa gcaggagact atatttcctt catgtcatgt ggtatggagc tgtatttaga
     9421 agacaaggta acagatgtaa atgatgtacg taatttcaga ccagaaaact ttccgcaatc
     9481 caatgttcat attttaaata atcacgaagt aaaaacgatc gatgccgata aaaaaacagt
     9541 aacggttact agcgcatcag ataacgaaac aaaagaatat ccttatgaca aattgatttt
     9601 gagttctggt gtcaaaccaa acaatctgcc agtaccagga gcagatctag aaaatgtcta
     9661 cttgatgcgt ggatacaatt gggcaaccaa aatcaaagaa aaattaaatg atccggctat
     9721 taaaaaaatc gctgtcgtag gtgcgggtta cattgggatc gaagcagcag aagcaagccg
     9781 aaaagctggg aaagaagtcg tattgcttga tgtgatcgat agacctttag gcacttattt
     9841 agatgctgag atgacagata ttttagaaca acacctaaat gaaaacggta ttgaagtttt
     9901 gacaaatgca aaaatcgaag cttttactgg taacggaaaa gttgaggcaa tcaaaacaag
     9961 tgaaaaagaa atcccagcag atctagtcat tcaagctgcc ggtgtgaagg caaatacaga
    10021 atggttaaaa gggatcgtcg acttagatga acgtggctgg atccaaacaa atgaatactt
    10081 gcaaacgaat cttccagatg tgtatgcagt aggagatgct acacttgctt attctatccc
    10141 agcacgcaaa aaaatgccga tcgctttagc gactgttgca cgtagagaag cacgttatgt
    10201 agtaaaacat ctttttgaag aagtaccaaa caaaccgttc ggcggagtcg taggttcctc
    10261 tgcattaagt gtctttgact atcattttgc agcaagcgga ttgaatagct ttacagcagc
    10321 aaaagcagga gtagatgtac gagtgtctta ttatgaagat acgttacgac caaaatatgt
    10381 accagaagaa caaggaaatc caaaagtgtc catccaatta ttctcggatc caatgaccca
    10441 tcaattgcta ggaggagcag tattatctac ctatgatgtt acaggtcaag gaaatgtatt
    10501 ggcactagcg atccaaaaca aactgacttt agaagaacta gcagaagctg acttcttctt
    10561 ccagccaggc tttgaccgac aatggagcat gttgaatcta gccgctcaac aagcgttagg
    10621 agaagaacca tttgtagaat aagaaaacga aatatttgaa aagaggttgt gaaaaagctg
    10681 ccttgcatca agtaataaga acagccaagt aaaaacagct tcttatgttt ttaacttggc
    10741 tggacttatt accgcagatg caagcttttg aacaccgttt attttcaaaa aagggactgt
    10801 gacacgattt gtgtaccgac ccccaaaagt tagaccaaaa aatctaactt ttggaggtcg
    10861 gtatttttta tggttaaata tacttttgaa cttaaattaa agatcgttca tgattatttg
    10921 gatggcaaag gcgattggac cgtaaaaatc cagatcaaga gattgaagcg gaaatgctta
    10981 aaattcggga gaagcacaag gattatggct gtttacggat gaccaatgaa ctaagaaacc
    11041 gcggtttctc tatcaataaa aagaaagttc agcgcttgat caagaagcta ggaataaaag
    11101 taacggcata cacgcgtaaa tcacgtcgct acaattctta tcgaggaaaa gt
//
LOCUS       DAZUKC010000076        11144 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000076 DAZUKC010000000
VERSION     DAZUKC010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.078"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..478
                     /locus_tag="ACG80T_002104"
     CDS             <1..478
                     /locus_tag="ACG80T_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841736.1"
                     /translation="ESVIILVYFQGGNLMIKKLFLLSILTLAMAGCSDSGLATELITK
                     STEATTEELKCGNDHSDYRGLNRRCMGILGRSNCKHRCSNRRRCRRSNRKIKRKIKRS
                     NLCCASYTDVEDAVLLNHVRRCSFMSREVGDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(539..904)
                     /locus_tag="ACG80T_002105"
     CDS             complement(539..904)
                     /locus_tag="ACG80T_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFS4841737.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(980..1225)
                     /locus_tag="ACG80T_002106"
     CDS             complement(980..1225)
                     /locus_tag="ACG80T_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4841738.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1295..1549)
                     /locus_tag="ACG80T_002107"
     CDS             complement(1295..1549)
                     /locus_tag="ACG80T_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841739.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1713..2036)
                     /locus_tag="ACG80T_002108"
     CDS             complement(1713..2036)
                     /locus_tag="ACG80T_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841740.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2223..2296
                     /locus_tag="ACG80T_002109"
     tRNA            2223..2296
                     /locus_tag="ACG80T_002109"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2257..2259,aa:Ile,seq:cat)
     gene            complement(2681..3178)
                     /locus_tag="ACG80T_002110"
     CDS             complement(2681..3178)
                     /locus_tag="ACG80T_002110"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFS4841741.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(3218..4027)
                     /locus_tag="ACG80T_002111"
     CDS             complement(3218..4027)
                     /locus_tag="ACG80T_002111"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4841742.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            4361..5218
                     /locus_tag="ACG80T_002112"
     CDS             4361..5218
                     /locus_tag="ACG80T_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFS4841743.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            5211..6113
                     /locus_tag="ACG80T_002113"
     CDS             5211..6113
                     /locus_tag="ACG80T_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFS4841744.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            6146..6496
                     /locus_tag="ACG80T_002114"
     CDS             6146..6496
                     /locus_tag="ACG80T_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS4841745.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            6545..7468
                     /locus_tag="ACG80T_002115"
     CDS             6545..7468
                     /locus_tag="ACG80T_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS4841746.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            7546..8439
                     /locus_tag="ACG80T_002116"
     CDS             7546..8439
                     /locus_tag="ACG80T_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4841747.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            8477..9040
                     /gene="amaP"
                     /locus_tag="ACG80T_002117"
     CDS             8477..9040
                     /gene="amaP"
                     /locus_tag="ACG80T_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFS4841748.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            9054..9242
                     /locus_tag="ACG80T_002118"
     CDS             9054..9242
                     /locus_tag="ACG80T_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFS4841749.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            9255..10157
                     /locus_tag="ACG80T_002119"
     CDS             9255..10157
                     /locus_tag="ACG80T_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS4841750.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            10189..10431
                     /locus_tag="ACG80T_002120"
     CDS             10189..10431
                     /locus_tag="ACG80T_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS4841751.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(10687..11058)
                     /locus_tag="ACG80T_002121"
     CDS             complement(10687..11058)
                     /locus_tag="ACG80T_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841752.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         3934 a         1918 c         2125 g         3167 t
ORIGIN      
        1 agaaagcgtt attattttag tctattttca gggggggaac cttatgataa aaaaactatt
       61 tttactatca atattaacgc tagcaatggc aggttgttct gactctggct tagcaactga
      121 actgatcaca aaatcaactg aagccacgac agaggagtta aaatgtggaa acgatcacag
      181 tgactacaga ggcttaaatc gacgatgcat gggaatatta ggcagaagca attgtaagca
      241 tcgatgtagt aatcgaagaa ggtgtagaag aagcaacaga aaaatcaaaa gaaagatcaa
      301 aagaagcaat ctgtgctgtg catcgtatac agacgtcgaa gatgcggtac ttttaaacca
      361 cgtcaggaga tgttcattta tgtctaggga agttggcgat gaaccaaaac tagagtacgc
      421 ttttagtttt gcacgtgagg atgaagataa ccctaaaact tacgacattt tagcttgatc
      481 taagatggtt aaagcttttt aatgaagtaa gaggagatag aaggcttttc tagttatatt
      541 aggtaagata aattcctttt tgctccaaat ttgctaaaca ttctgctaga taaacttcat
      601 cgtgttccgg ataaatcggc acgttgcgct ctacagtagg taaatcaaaa aatgcttcgc
      661 atgatttgcc acgatacata ggatttttcc actctggtgc tgggctgtaa ccacgttttt
      721 ccatttcatc catgacaagc tgatgatatt ggaataatct ataaggagaa tagtgaaaaa
      781 cataatccac agtagcatgt ttctttcccc agccatttcc tcttaatgca cagcattccc
      841 gatgttgtcc taaaagctgt tgacgaggaa gctttgaaat caatttttca tgccataatc
      901 gcatagaaat gtctccttta tatatagatt gttaacctta gacgttaaat gaaaaaacca
      961 gcaccaaggc tggaaaaaat cacttcagtt tgcttgctgt ttctgagcga ccaattaggt
     1021 aatcaacgga aatatcgtaa aagtcagcga tatagattaa ttcaatgatg cttggaattt
     1081 cttctccttc ttcgtactta cgatatttga ataaaggaat acttaacgcg ctagaaacag
     1141 ctacttgaga taaagatcgg ccagttctta agaaattcag acgacaacgt aaagtatcca
     1201 gtaaaatagc ttcttcgata ctcatttaat cacctttcat tatagtagaa tagttataat
     1261 gactatagaa agctacacat gcaatcaata gtactcaatg ttttttattt aaacgtatca
     1321 aataaagaat gacgatcaat aaagacaaaa ccagtcctga taattcttct ataaaaacac
     1381 tatcgggttc aaagtagttg taattaaaaa ctttccacat aaagtagata gccggaagag
     1441 tcaaaatagc taaataaaac cgttctttgt tccaattcat aagataaaca atacttgcga
     1501 ccagtgcgac cgcaggcacc caaaacaaaa ctctcaccaa cgtaatcatg taacggactc
     1561 ctcaacgatt catataataa aagataacat gttattgcat acatcacaaa tgaaatgcta
     1621 ggaaaataaa agaagaatag atcaatgaaa aatgaaaaag cagaacagtt ttctgctgat
     1681 aagcaggaac tgttccgctg aatgtgtctt atctatcgag aaaaaatcaa tggattgata
     1741 tctaagtaat tcaatacata taaaaagagg acgatcgcat tcactaaaaa ggaaagaatg
     1801 atgatcccat agcgaatgga aacatattta acaaatactt ttgtttgaat acgggcatat
     1861 ctttttcctt tatagctgag catcatgaca agaagcaaaa gggtaatcac accttgaaaa
     1921 atcattccaa taaacagaat tcctgaggtt tgataattta attggagaaa ataagctgct
     1981 gccgcaacta aatcgaataa aatcgcaata aatggcaaaa taatcactcc taacattgag
     2041 aataaataaa agaataacat tttttctaaa ataagcaaat aaaaattaaa gtaacaaaat
     2101 agttatgaca caagtaaaag aaataagaat aacaatatta ttttacttat attttgaaac
     2161 ttgtgagagt ttgtcattcg tgctatagtt gaaaagtaac aaaaataaat tttatttatg
     2221 atgtaccttt agctcagttg gttagagcag acggctcata accgtccggt cgtaggttcg
     2281 agtcctacaa ggtacataag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca
     2341 gccaagtaaa aacagcttct tatgttttta cttggctgtt cttattactg cagatgcaag
     2401 cttttgaata ccgtttaagg aagaggttgt gaaatcagcg ttttgacctg agtattagct
     2461 taacaaacgg aaaaatagtt cctcatattt tgccgtttgt taagctaata ccgagggtca
     2521 gctgattgaa caccatttaa gaaagaggtc gtgagcctgc cgtttcactc tgtgtcataa
     2581 aaaagccaat ctactttgat agaaaaagca cgtaggacta aaacagttct gcgtgctttt
     2641 tttattgtgt gttaaatatc gtaaatgaag atagatattt ttatgattcg acgatagaga
     2701 cgttgaaatc tttcaacagc tctaaagcgg attctccgtg ttttctactg gagactaaca
     2761 ttggatcaac aaaaaagtct gcttcaggaa aagcactctg caggcctatc agattagaga
     2821 gtacacacaa gtttgtttca atcccgacta cttcgatctc tttttgttct tctttgcttt
     2881 caaataaagt ttcctggatt tcagctaaag tttcaggtgg aatcgtaaag taatactttt
     2941 taatcactcg ttcattttct tgtggagaaa gaagagggat caattgcaaa tcttctcgtt
     3001 cttcttcttt gtccttcagt tcaactggta tatcccgtgt gaagagaata tattcattat
     3061 tttggcgtgc tttagctaat ctttgagcaa tacgttctgc caattgatct gactctggaa
     3121 tataaaattc gctgtttgga tcaagaatat gattttgcat atcaataata actaacattt
     3181 tctttcctcc tacattcata tcgtcatttt attcttttta atagaagttt tttaaaatat
     3241 aagcaacacc agcttcttca ttcgtcagtg ttacttggtt tgcaatttct tttacttgtc
     3301 gctgtgcatt ccccatcgca atactcaacc cgctgtaatt caacatagca atgtcgtttt
     3361 cttgatcgcc aaaagccatg atgtcactcg attcgattcc aaggtaatcc gccagaaaaa
     3421 caagtgaatg agctttagtg atacctgtag gcgtgatttc taagaaatgc ggatcagaga
     3481 atactgattg gataccatct gcttgcagat ctctttgtag cttctgtagt ttttcttcac
     3541 tcttatctgt taatgctaat ttaagatagc taagagaagg atcttccaac atatttaaaa
     3601 tttgatcaaa agaatggttc tgtaaattga aatgttcata ataagggtca tctttgacta
     3661 gatttgattc atcgaatgtc agctgatcat cttgggtttc tcggctagca atgatggata
     3721 ttttcgcttc ttgagcatag aagaaggctt ttctcagtgt atcgctatct atttttgaac
     3781 gtctgagaac tttcgccatt tttccatcct tcatttcagc aatcagtgaa ccattggaag
     3841 aaatcaaata gccaccgatt cctaattcgg aaaaaatatc tttagcagaa atgctgttcc
     3901 ttccggtagc gacggttacg attccgccat tttcatcaaa tttccgaagt gcttggatat
     3961 tttcttctgg tatctttcca tttcgatcaa gcaaggtacc gtctaaatca atcgctgcta
     4021 gtttcatttt atcttcctac tttcttttta catatctatt ttcctgttac cttaagatta
     4081 aatgagaatt taaaaaaatg aaactaactt gctttgtcat tatgaatttt ttcaaaaaac
     4141 attcttaaga aaaaggtggc tatatatagt agtttttggt caataccttt gaataaaaag
     4201 ccatgaaaaa cgaatagtta gcgcttaccg atgcgttttg tgataaaaaa attttaaaat
     4261 attcaatgaa aacaaagcat atattttgct ttaaaaccaa atgaggaata ttctagatgt
     4321 agaacaggtt agaaaaagat tagaaaaagg agtgattttg atggagaaga gatacggcgt
     4381 agttcctgtg gtaactgctt cagacgaaaa ttatgctcct tacttgagcg tgatgatcgc
     4441 aactgcatta gaaaattgca ataaaacaag acgcataaaa ttttatgtga ttgacgatgg
     4501 tctatcagaa tatagtaaag aaggattgga agaaacggta aataaatatt caagtaatgc
     4561 aagtatccaa ttcctgacag tggaaaaaga tatctacgaa gacttcctag tcagtgacca
     4621 tatcacaact accgcttact taagaatttc tttaccaaac ttattagcta aagaggatta
     4681 taagaaagtc ctttacttag attcagatgt gttagtacta gacgatatcg taaaactata
     4741 tgatgagccg ttaaatggaa aaacaatcgg cgctattatc gatccaggcc aagtgaaagc
     4801 tttggaaaga ttaggaatcg attccgatga cttgtacttc aattcaggag tgatggtgat
     4861 tgacattgat caatggaata aaaaagaaat tacagaaaaa actatccatt acctcagtga
     4921 aaatggcgat cgaatcatct atcacgacca agatgctttg aatgctgtac tttatgaaga
     4981 ttgggaacaa cttcatccaa aatggaacat gcagacttcg ctgatttttg aacgtcatcc
     5041 agcaccaaat gaaaaatatg aacgtcagta taaagaagga aatgaaaaac cttcgatcgt
     5101 tcactttaca ggacatgata aaccttggaa tacgttgaaa gatcatccgt ataccaattt
     5161 atacttaaaa aaattagcac acagtactct aacgaaagta ggcgagatca atgaataaaa
     5221 aagaaatagc agtagtcgca agttgtaaca caaaattcgt accacattta gctgcattat
     5281 ttgtctcagt attagataat tgcaacccgt ctaaatttgt tcggttctat gtgattgacg
     5341 atgatatcga ttttgaaagt aaacagcttt tacgcttctc agtgaaaaac gcacgaatga
     5401 actcagatgt ggaattcttg aaaatcaata aagaattctt tacaaatgtg gtaatcagcg
     5461 atcgtattcc agaaacagct tattatcgta ttgcgattcc ggaattgttc cgcggcacag
     5521 aagttgaacg tatcttatat atggattgcg acatgatcgc tttacaagat atctctaaat
     5581 tatggcgtct tgacttcggt gattcaatcg tagcagctgt tgaagacgct gggttccatc
     5641 aacgactgga aaaaatgaaa atttcagcta aatcaatgcg ttatttcaac tcaggtttga
     5701 tgctgatcaa tgtgaaaaaa tggttagatg aaaatattac gcaaaaagta ttagatttca
     5761 tcgaacataa tccagaaaaa ttacgattcc atgaccaaga tgcattaaat gctatcttac
     5821 atgaccgttg gttaccactt catccgcgtt ggaatgcaca aggctatatc atggcaaaag
     5881 caaaaaaaca tccaacagca gcaggtgaac gtgaatacga agaaactcgc aacaatcctt
     5941 acattatcca tttttcagga catgtgaaac cgtggagcaa ggattttgaa gggccaacga
     6001 agaaatatta cgaaaaatat gcagggatga cggctttccg ttgtgtagct aaattcccta
     6061 aatatccaaa atatgcaaaa gttcaaccaa aaacagatgt agttgttaaa taatagaaga
     6121 caacaactga agagggggag taggaatgga aattacaatg tctaaaaatg cagtagagac
     6181 gttaattgaa aaggttgggg aaaatacaaa aatcgcactc gcgcttatca atgattccga
     6241 tccatttcta agggacaaag gtgcttttgc aaaaggcagc ttttttcaaa tcattccttt
     6301 tgtttcggaa tttggagaat atgcgactaa aatcgagcat ccattattgg acatctatac
     6361 atctaaatta gagcaaaatt atttcggtaa acgattgaac atggacttca acaaacagct
     6421 tgacagcttt tcattagaaa atgaaatcgc agtactcgat tataatatca aattaaagaa
     6481 ctgcttcttc agctaatagt ttcgagaaag tccaaaaagg aggtatacat tgagatacta
     6541 ctttgtggat gaagaaaaga atgtaataga aacagatgaa aaaaaatata attggctcgt
     6601 acttgatagt gcaaagcccg aagagattga gcaagcaatc gaagaattcg agcttcctga
     6661 tgatattttc gtaggaaccg aatatccgga agaagtttct cggatggagc gcctttatga
     6721 gacgaaacta gagcatccat tttctttggt agtgattaat ctagatcagt cagataatcg
     6781 tatcgaaaaa aaattaacgc caatatcttt tgtactttcc gataatctct tgattacttg
     6841 tatcgataga aaaaccgaat ttgtggatcg gctgctgaca aagcatggaa atcatttaaa
     6901 ctcgtttgaa aagatcatca cgtatacatt attagatatt tacacccatt acgtgaaaga
     6961 actaagagag atgaaaaagc gaattgacgc tttggataaa gcagctcgta aaacaacgga
     7021 aaatgaagaa ctgtataaac aggcagatct tgaaagagac atcgtatata tcgatcatac
     7081 cttaagagat caacgggaaa caatggatca attatggaat tctgaaactt tcatcaaacg
     7141 tttagacgat gaacagctac tctatgatgt ccatttgaga cagcgacaaa cagaaaaaat
     7201 gatcgagatc tatcgagatt tattagagag tattggtgat ctattcaacg ggatgatgga
     7261 caataatcta aatcatatca tgaaatatct agattcagct gcattaatca tttccgttcc
     7321 ggcgttgttc gcagggattt ggggaatgaa tacaggagga ttaccgggta aaagttctgc
     7381 tctaggcttt atactcgtag tctttggctc tttgatagca gcagtagttt taggttacca
     7441 tttataccgg aaagattata caaattaaag aatctattta aaacaggtat caaacaaaga
     7501 aatttgtttt ggcaatacaa tttaaaaact aggaggaata cagcaatgac agaaccaaaa
     7561 ttaatcgacc cgcgaaactt gtaccatacg gagaaatttc ctaaacaaga tcaggataca
     7621 ccagctttac agcagaatat gattcctgtt cctgattgtg gagaagaatc ctatgaaggc
     7681 aataatcaac tagaaaaccg ccgtgtacta atcactggtg gagattcagg gatcggtcgt
     7741 gcagcagcga ttgcatttgc tagagaaggg gcagatatcg ctcttcaatt cttcccagga
     7801 gaagaaaaag atgcagagga agtagcaaaa tacgtgaaag ttgcaggacg taagattgtc
     7861 ttactacctt ttgatcttcg tgacgaacaa gcacctaaag aaatcataga taaagccgtg
     7921 agtgaacttg gcgggctaga tacgatggta ctaaacgcag gacagcaaat ttcttgtcag
     7981 tctattgctg agttaccact tgagcaagtc aaagacacat ttatgataaa catcatcgct
     8041 atgtttgctt tagtgaaaga agctgttccg catatcccag caggaggagc aatcgtaaca
     8101 acaacttctg ttcaagcatt caatccaagc gaacatttat tagattacgc agcaacaaaa
     8161 gcttcaatcg ctaactttac gatcggttta gcaaaacagc ttgcacctaa aggaatccgt
     8221 gtaaatggtg tagcacctgg tccaatctgg acaccattac aactagacga aggacaacct
     8281 tctgattcga tccctgaatt tggtcagcaa agtttactag aacgtgcagg tcagccagga
     8341 gaattagcac cagtttatgt tttcttagca tcgaataaag caagttatgt tacagcccaa
     8401 atttacggag taactggtgg agaagcgatc aacttataag aagttgatcg tttcacccct
     8461 atgaggaggt gcgaaaatga ataaaggtgt aaaaacgatc ggagtgatcg gaacattact
     8521 tttgctatct gtgcttttgt ttgcgatact attaagtgca acctatccat tgccttttca
     8581 gctagagaag tttcgattct tttccatcac taattcatac gtgcaacaat atgtattttg
     8641 gcttgccgtt gcatttgcta ttttgacaat tatcacgtta cttgttttcc tgttttatcc
     8701 aaaaacaatc ggaaactttc tactgaaaca tggtgatggg gagttgacgt tggataaaaa
     8761 agcaattgaa ggaatggttc gttctcactt gcatgaagat gaattcatcc attcaccaaa
     8821 agtaaatgtc aaagcaacga aaaacaagat cgccgtcaat gtaaaagggg agttgaaacg
     8881 aacttcagca ctcattggta aaacaggaac attgatgaaa gaaatcgaaa atgaagtaca
     8941 acacatattg ggaacaaaag agccagtgaa agtatcggtg aaatacacag gctttgaaga
     9001 accagctaaa cgaagcaatg accgcaaacg agtagaatag ggggggacca aaaatgaaag
     9061 aactattttc gacatacaag ctccctatca tctttggggt acttggattg atattagccg
     9121 ttttatggat caccgtgggc tttttcaaga ccttattgat cttgatcatg gcagcgatcg
     9181 gcgtcgctat cggtttttat ctgaaacaaa cgaaaatctt agatcactat tttaaatctt
     9241 aggaggaaac atccatgaat aacaataaca aaaataataa caataaaatg aacaaccaaa
     9301 aaccagtgtc tgcacatcca gacccaacga caccaaacca atcaaatggt gtgaacgatg
     9361 aaaagaacaa aacagcacca gtatctgcac atccagatcc agtagtccct aacgttcaac
     9421 aaggagtaga agagaagaaa catgatgcag aaaagcatgc acctcatcat ccaggaactc
     9481 cagaatctaa agttccagaa caacatgatg caatcgatcc ttctaaacct gtggaagagc
     9541 aaaaacatcc ttctgaccat gcgaaagcac aagaaaaact tagttctgac cacacaaacg
     9601 gacaaggaaa acttggttct gatcatacaa ataaccaagg aaagcatgat tctgaacagg
     9661 aagattcaat gcgcggtgag ctgaattatg aagataaagt tgtgcaaaaa atcatcggaa
     9721 tcgcaattga acaagtagac ggtttattat ctgcaaacgg tggtttcttc tctaatgtgg
     9781 caggaaaact tgtgaataca gataatgtaa cggctgggat cgaaacagaa gtcggtaaaa
     9841 aacaagtagc agttgatatg gatgtcattg tggaatatgg caaagatatc gaaaaaatct
     9901 ttgaagaaat gcaagaagta atcggcaaag aagtaaaaaa tatgacacat ttagaagtga
     9961 tcgaagtcaa cgctaacgta gtggacatca aaacaaaaga agaatttgaa aaagatcaag
    10021 aaacggtgca agacaaagta actgacgcag cgaaaaaaac cggtgaattt gcttcgaatc
    10081 aaacagataa agtaaaatca gcagctggaa gcggcgcaca aaaagtaaaa gaaaatacag
    10141 aaccacgggt tcagtaataa ggaagtaaaa cattaggagg aacgaaacat gggatttatt
    10201 tggtcactta tcgtcggagg aattatcgga gcaattgcag gagcaatcac gaataaaggt
    10261 tcttcgatgg ggattattgc aaatgtcgta gcaggtttag ttggatcagc acttggtcaa
    10321 gcattattag gttcttgggg accaagtatt gcaggaatgg caatcgtgcc atcattgatt
    10381 ggtgccatca ttttagtact aatcgtatca ttctttgctc gaaaactgta aaaagacttt
    10441 taaaaagagg tcgtgaaaaa gctgttcttc atcaagtaat aagaacagcc aaacaaaaat
    10501 agcttctcat atttttcatt tggttgggca tattatcgaa gatgcaagct tttgaacact
    10561 gtttatacgg aataagaggc tgtgacaaga cttttgtcac agcctcttat ttatgcgggc
    10621 aaattcccaa ttagattttt aaaagttttt ccttaaatgt gtctttattg atacagagaa
    10681 gcttctttaa taaaacaatg cctccctaaa ttttcttgag ataggaaagg tgaacataca
    10741 aaataatacg accactttga aaacccagat gatccacaaa tggatcgtat gatactgatg
    10801 aagtaaaaag caatggatca gaaactcaaa gagattgaaa aagacaagga agaaataata
    10861 tctctttttt actcttgtct gttttagcca ataatagctc gtaaagtaat ctagacaaac
    10921 aaatagaagt aaacaaagtt tcgtaagagc agcaatcgta tcatgttgct ctgctagacg
    10981 attgacgagc aggaaaaatg ttggtaatgg aatatagatc acgagtaatg aaagaaaaaa
    11041 gtaaacaata aatggcatac tattcctcct aattgtgtga gttcgccctc attttaaaat
    11101 aacgatattt gtttgtgaag ctttttcgtg tcgtaacaaa tgtt
//
LOCUS       DAZUKC010000077        11138 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000077 DAZUKC010000000
VERSION     DAZUKC010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.079"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACG80T_002122"
     CDS             6..305
                     /locus_tag="ACG80T_002122"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4841753.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACG80T_002123"
     CDS             378..1739
                     /locus_tag="ACG80T_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFS4841754.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFLSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLLFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGILTMPIYLYGATYLAPILSDSAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACG80T_002124"
     CDS             1761..2021
                     /locus_tag="ACG80T_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841755.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACG80T_002125"
     CDS             2035..2787
                     /locus_tag="ACG80T_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFS4841756.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACG80T_002126"
     CDS             complement(2881..3486)
                     /locus_tag="ACG80T_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4841757.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIEQAREWTDHLLTK"
     gene            complement(3542..4516)
                     /locus_tag="ACG80T_002127"
     CDS             complement(3542..4516)
                     /locus_tag="ACG80T_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFS4841758.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIK
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DCPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4894..5613)
                     /locus_tag="ACG80T_002128"
     CDS             complement(4894..5613)
                     /locus_tag="ACG80T_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4841759.1"
                     /translation="MPKYEEIANILRERIRSKEYPPDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5740..7011)
                     /gene="celB"
                     /locus_tag="ACG80T_002129"
     CDS             complement(5740..7011)
                     /gene="celB"
                     /locus_tag="ACG80T_002129"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFS4841760.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7164..8561
                     /locus_tag="ACG80T_002130"
     CDS             7164..8561
                     /locus_tag="ACG80T_002130"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4841761.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWNHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSRLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKAIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGTIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8642..9082
                     /locus_tag="ACG80T_002131"
     CDS             8642..9082
                     /locus_tag="ACG80T_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS4841762.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9229..9555
                     /locus_tag="ACG80T_002132"
     CDS             9229..9555
                     /locus_tag="ACG80T_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS4841763.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9556..10305
                     /locus_tag="ACG80T_002133"
     CDS             9556..10305
                     /locus_tag="ACG80T_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFS4841764.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10331..10741)
                     /locus_tag="ACG80T_002134"
     CDS             complement(10331..10741)
                     /locus_tag="ACG80T_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS4841765.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNK"
BASE COUNT         3533 a         1947 c         2291 g         3367 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttcgaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcctgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtctta ttgctgcttt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattacca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcctc acgatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgattcagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaaggaccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcaaaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgggagat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc tcgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccaatc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa aaatgaagta gcatgaagag
     3541 attattcacg ttttttattt gcatcacttg tatcgaaagg acggacatag gcttcaatgg
     3601 cttcacgacg ttcttctaag aatggaggaa gagatagttt ttctcctgct gattctgctg
     3661 gttcatcttc aaggaatcca ggacaatcag ttgctaattc aaacagtacg tgatgagcgg
     3721 ctaggaagta ctcagattgg aaatagaaac gatcgacgaa accagaattt ggcaattcaa
     3781 tcttattgat tacttgaatc cagtattcca atgcttcttt gtctgctaca cgcaatgcta
     3841 agtgatggat atttccaaat ccttcgattg cttttggcaa gtctgtccga tgttcaataa
     3901 tgatgcttgc accatttccg ccttcaccga tttcaaataa gtagaaatct cctgaatggt
     3961 ctgtttcttt gaatcctaag attttttcaa gaatcaagcg gatatgctct aattgactga
     4021 cacggacaaa gacaggtcca agtcctgtga tcgcaaactc tgtcggtaca ttgctgtttt
     4081 tccaaggaat gccagctgct acaccttgat tgttttcgtc agagatcaat tgataatact
     4141 gattatcgaa atcttcaaat tcgatatatt ttttgccgaa acgttcattg attgctgaat
     4201 gagtaacgtc atatttttca aaacgttcca accaaaagtc taatgctgcg tcacttggca
     4261 cacggaaaga agcacgagag atcgtatctg ttcctttttt tccttttggc tgatttggaa
     4321 aatcaaagaa agtcatatct gttcctggcg caccagtatc gtctgtaaag taaagatggt
     4381 aagttttgat gtcatcttgg ttaactgttt ttttgattaa gcgcattcct aaaatatctg
     4441 taaaaaagcg gtagattttt gtagcgctag atgtcattgc tgtgacgtgg tgaattcctc
     4501 tgatttgtgt atccatgata aatttcctcc tgtcaactta caaaaagtaa gtagttgatt
     4561 tagacatagt atagagggtg catctctatt ttgcaattaa tttgtcttct aaagtatcag
     4621 gaaggaatca agaatggaaa agaggaggga cggatcaata aaataaaaag acttacgact
     4681 agctgtatcg atttcaaaaa aactagcttt aaaaagctaa ctttagaatc attacgggtg
     4741 gtcataagtc tttttatgct gagttatttt ttcatttttg agctaatgct taggatagga
     4801 tagaatgttg atttcacgat ctcttcttgt tacttgaaac tgcttactac tgtcacgatc
     4861 tcttggtaaa cggctttttc acaacctctt catttaagtt gaacgcacat ccagcacaga
     4921 atagttccga acatcaaatc ggtttcggct ggaagaatat tcaatcggct gtccatcttt
     4981 gagatagacg acttgctgaa cttccagtac aggatcgttt tctgcacagt tcaaatattg
     5041 ctgatcatat tcatcacttt tatctgcaga aatagcacga taagcccctg caaaatggac
     5101 attcaattct tgatgaagat gatcatagat agaggctaac aaatgttctt tttttaaacc
     5161 cggcacaaga tggactggca taaaagtatg ctctaaaaca tatggctcgc catctaagat
     5221 ccttaaccgg tgaatattgt agacaggttg ctcggcatcg atcatcaatc gttcttggat
     5281 atattgatta gggaattcaa catcaaaatg aatgatttga ctcttcaaag aacgatgtct
     5341 gcgtttcatt tggagactca aaccttcata ctcgttagcg ggcgatgtgt ctttttgcat
     5401 aaatggatga ttcaagattt tcgttcctga tcctcgctgc gaatagacaa gtccttccat
     5461 tgctaagata ttgatggctt ttttgattgt catccggctc gcaccaaatt ctttgaccaa
     5521 gtccacttga ttaggcaaaa aactatcggg aggatactct tttgaacgga tacgttctct
     5581 taaaatattt gcgatttctt cgtattttgg cacgttattc acctcttcta gagtataacg
     5641 aaagattatg cttccgacaa ctcgaagtag catctggaac attgtttatt cgaaaaaaga
     5701 ggtcgtgaca agtttttgtc acaacccctt aatgatctat taagcaatat ctaaatctgg
     5761 ttgtttatct agtactttca ggaatgggta ccagataaag gcaacgatga caaagtcgat
     5821 gacttgcagc ataccaccaa gccatgaatt tgttgctaag acaccgcttg caatgattgg
     5881 cacagtccat ggaacagaga cgccagttgg tgcagggaca agtccagcag ccattaccaa
     5941 ataattgaac gtcgttacga ttagaggtgc cacaacccaa ggaataagga ttgttgcatt
     6001 caatacgatc ggtagaccga agatcgctgg ttcatttaca ttgaaaatac caggtgctaa
     6061 agctaatcgt ccaacatcac gatactgttt ctttttaaga acaaaggcca tcaataaaac
     6121 gacagctaaa gtcattccag agccaccaat accaacagtg aaggtttcca tgaatggttt
     6181 tgtgatgatg tgtggtaatt cttttccatg ttgataagct tctaagttat caagcatcag
     6241 tgtattccaa atcgggtcca ttacagagtt gacgatgatt tgtccgtgaa gtccgaagaa
     6301 ccataagaat tggacaaaga acaaagcaat caatgttgca ggtaaactac ttcccaaacc
     6361 agttaatggt ttttggatca cttcataaac gacgtcatgt aagttagcat tgaaaatacc
     6421 agtcatcaca gcattgatta ctaagaaaat agttagtgtc actacagcag ggatcaaagc
     6481 ggcaaatgat tttgttactg caggcggaac tccatcaggc attttgatct gccagccgcg
     6541 tttcgtgatt ctgcagtaga tttctccagc caagaaagca gcgatcattc caatgaacat
     6601 tccttttgcg ccaagacgat caagtgaaag cacaccagtc acatcttcac cgccagcggt
     6661 tttcattgca aaaggcgtaa gaatcaagaa gctggcaaaa gacacggcac caccgaaaat
     6721 accttcaacg tcataagatt ttgataaata atagccgata ccaaaagtaa caaaaaccga
     6781 catgatgctc attgttgcat tttgcccatt accgaataag ttcgataaag ttccctttgt
     6841 tgcgtcactg aaaaaaggca agttattgat aacgacaacg atcgaaccga acatcgttaa
     6901 agggaaggaa agcatgaaag catcccgcag cactgtaagg taacgatttt gccctaataa
     6961 attagctaag ggcatgattt tctcagaaag cttgtccata aaaccgttca tttttattca
     7021 cctcaaattt atttgcaatc gattacatac aaagtataac gccaaaaatt ttaaatgtct
     7081 agtcattttc ataaaaagta ttgatttatt ttttagggtt cgttatgatt acgatatcaa
     7141 gaaaaaagag aggaagtaaa aaaatgaaaa cctacacatt cccagaaaat ttttggtggg
     7201 gttctgctgc tagcggaccg caaacagaag gaagagtaag tgatgatggt aaaggagaaa
     7261 atatttggaa tcattggtat gaattagagc ctgaaaaatt cttcgatcaa gtaggacctg
     7321 aaaaaacttc gcaagtttac acaaaataca aagaagatgt tcagttaatg aaagaaactg
     7381 gccataacac atttcgtaca tctatccaat ggagtcgttt gattccagaa gggacaggtg
     7441 aaatcaatca aaaagctgtt tctttttatc gagattattt tactgaattg ctggataatg
     7501 gtatcgaacc ttttgtgaac ctttaccatt tcgatatgcc actaccactg caagaaaaag
     7561 gcgggtggtt atcaagagaa acggtcgacg cttatgtttc ttatgcagca gcgtgctttg
     7621 aactgtttgg agatctggtc aaaaaatggt ttactcataa tgaaccaatc gtacctgtag
     7681 aaggaggcta cttgtatcaa ttccactatc caaatcatat caatatgaaa gaagcggtcc
     7741 aagtaggcta tcatgagaat ctagcaagcg caaaggcaat caaagcctac catgaaggcg
     7801 gacatgatgg tgagatcgga atcatcttga atctgacacc aagctatccg agagatgaaa
     7861 cgaacccaga agatgtaaaa gcggcgaaga tcgctgacgc attcttcaac cgatcattcc
     7921 ttgaccctgc agtgaaagga gagtttccac aagagttgat cgaaattgtc aaagaattgg
     7981 ctataatgcc agagatccat gaaggagacc tagaactcat ccgtgaaaac acgattgacc
     8041 tgctaggaat caattactat cagcctcgtc gaatcaaagc aaaagaaact ccaatcaatc
     8101 acgaacatag ccccatgcca gatgattatt ttgataatta cgacatgccc aataaaaaaa
     8161 tgaatccata tcgtggatgg gaaatttacg aaaaaggaat ctatgatatt ttaacgaata
     8221 caagagaaaa ctatggtaat atcaaatgct atatttctga aaatgggatg ggggtcgaag
     8281 gagaagaacg atttgttaac agcgaaggaa ctattgaaga tgattaccgg attgagtttg
     8341 ttacggatca tttgaaatac gtccatcaag caatccaaga aggaaccaat tgtgtgggtt
     8401 accacatgtg gacatgtatg gacaactggt cttggacaaa tgcttataag aatcgctatg
     8461 gctttatcgc tgttgatttg gcacaagacg gtaaacggac gatcaaaaaa tcaggtcgct
     8521 ggtttaaaac ggtctctgaa aaaaacggat ttgatgcctg atattttttg ataaaacgtt
     8581 cacaaaatgt ttgttctaac actgtaatcg tgctacacta acagtgaagg aggcgtttac
     8641 aatggtagat acttatcgta atattttagt agcaattgat ggttctgaaa aggcagaaaa
     8701 agcatttctt gaggcaatca cgattgccaa acgaaatcag gcaacgttgc acatcctgta
     8761 tgtcaatgaa gtaacaggca attactttgg tgattttgca tttgttacaa caagccttca
     8821 ggaagaattg gatgaggttg cggaaaacca gatgaaagaa catcgaaact tggcaattga
     8881 aaaaggcttg acagatatcg agacatatgt tctttacggt tatccaaaga cactgattgc
     8941 taatttccat gaatcgaaag aaccaatcga tctgattgtc atgggggcga caggtctgaa
     9001 tgcggtagaa cgtgcactag taggttccac tacttcctat gtagtcaacc acgcatcatg
     9061 taacgtcatg gttgtaaaat aaataaatta agggaacaga aagcaaaaaa gcataacgct
     9121 tcttttgctt tctgttcctt taattttttg taaaaaatta ctttacctga tgaagtaatc
     9181 atgctattct aattacaacg acagatggag taatatggag gaaagaaaat gatttcaagt
     9241 gatggaatac gcggatacaa tgatgcgatt attttatcga tccttaacaa aaacgattct
     9301 tatggatatg aaatttctaa aacgattcgt gagaggacaa atgattccta tgtgatcaaa
     9361 gagacaacac tttactccgt ttttgcccgt ttagaaaaga atcaactgat tgtttcgtat
     9421 cctggagaag tgactcatgg aaaaaagcgg acatattata aaatcactga aatagggcgt
     9481 aatcttttag aagaaaaaaa gcaagagtgg ctgacgacga aagacgtagt cgatcaattt
     9541 ttggagggaa attaaatgga tacaatccgt aattatttag acagtctttt tatcggagta
     9601 cctcaaagta ccgagataga taagctgaaa actgatttgt tggcaaatat ggaagaccat
     9661 tatcatgaat tgatgggaga aggaaaaaat gaacaggagg ccatcggtac agtaatttcg
     9721 acatttggtt caatcgatga attattagaa gaactagatg tcgaaaaaaa acatcaagct
     9781 gacgagacgg aaacaaacac agcttctatt tatttaagcg aagcagaaaa ctactggaaa
     9841 gaatacagag cggcaagtct ccaagtggca tcaggtgttc tattcattag cttatctttt
     9901 gcaagttttc ttttcttttg ttcagcaggg tatgtgttca tgggtatttc ttgtctgatt
     9961 ttcggtatag cattagctgt cggttttttt attgcttctg gtatgaaaat cacacgttta
    10021 aatcactttt tgcaccatcg gaaaatacct gaaaaagtat tagcagaagc aaaagaaaaa
    10081 gaagaagagt atcagcgaag ttttggcttc tcactgattg caggaattgg attatgtatt
    10141 ttttcacttt tcccactttt ggcttcttta atgtggtata tggatggtag tatcggtgcc
    10201 tctatatttt ttgttacggt aggaacggga gtcttcttga tcatttatgg cagtctggtc
    10261 cgtcattctt atcgtcaatt cactcaaagt gcttattact ggtgaatgta ttcagatttg
    10321 taaaaatatt ttatttattg cttacccatg taaaatcgtt atagacagat ttcagttgac
    10381 ttctgcgcca ttgtacaggg ataaaaagac gcatactatc ttcattgaat ccgactaata
    10441 atttctttcc gtcagtaatg atgggacgtt gtaacaaagt aaaatgcgag acgatcagat
    10501 cgacaagctc tgaggtcgta cattggtcga tttgatcttt caaattttta taatcaatcg
    10561 agcgtttagc taatagatcg tcaaatccgt tttctgttaa tgacaataaa taaaatatct
    10621 ctttccttgt taaaggctgt ttaggtgtga aaattcgttc tctgtaaggc atttgatggt
    10681 ctttcagcca gtttgccatc agatggtaac tatgtgtgcg agaagaatag atcgtaagca
    10741 taaaaaaact ccttaaatag tccattaatt tgttagatct gttgagataa ataataggta
    10801 aggagctgtg cataagcatt gatcaaaaca acccaaacat cattagaaat aactttctaa
    10861 agtcttaaac ttgcgacgag aaacaactag actaatagaa aaaatgtctt agccatttgg
    10921 cttcctgttt gtttttttgc ttatgctgca gctcctcggg aaagggttct aacaatttga
    10981 agaaggttga tgattgatct agtttaaatt gtgtgaaatt tgaaatctgg tgtttttact
    11041 agatgaatca cctaacaact atgattataa cttatagaac ttctgttttg tacttggttt
    11101 tgttatagtt gttataaagt tttattttat tacagaac
//
LOCUS       DAZUKC010000078        10820 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000078 DAZUKC010000000
VERSION     DAZUKC010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.080"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            185..1183
                     /locus_tag="ACG80T_002135"
     CDS             185..1183
                     /locus_tag="ACG80T_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS4841766.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1295..1804
                     /locus_tag="ACG80T_002136"
     CDS             1295..1804
                     /locus_tag="ACG80T_002136"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFS4841767.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1834..2493)
                     /locus_tag="ACG80T_002137"
     CDS             complement(1834..2493)
                     /locus_tag="ACG80T_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFS4841768.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2495..3259)
                     /locus_tag="ACG80T_002138"
     CDS             complement(2495..3259)
                     /locus_tag="ACG80T_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFS4841769.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3267..3944)
                     /locus_tag="ACG80T_002139"
     CDS             complement(3267..3944)
                     /locus_tag="ACG80T_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFS4841770.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3981..5366)
                     /locus_tag="ACG80T_002140"
     CDS             complement(3981..5366)
                     /locus_tag="ACG80T_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFS4841771.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5576..5660)
                     /gene="ffs"
                     /locus_tag="ACG80T_002141"
     ncRNA           complement(5576..5660)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACG80T_002141"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="ACG80T_002142"
     CDS             complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="ACG80T_002142"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFS4841772.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6305..7162
                     /locus_tag="ACG80T_002143"
     CDS             6305..7162
                     /locus_tag="ACG80T_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4841773.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDGTEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7243..7578)
                     /locus_tag="ACG80T_002144"
     CDS             complement(7243..7578)
                     /locus_tag="ACG80T_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841774.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7645..9015)
                     /locus_tag="ACG80T_002145"
     CDS             complement(7645..9015)
                     /locus_tag="ACG80T_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFS4841775.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9093..9629)
                     /locus_tag="ACG80T_002146"
     CDS             complement(9093..9629)
                     /locus_tag="ACG80T_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4841776.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            9938..>10820
                     /locus_tag="ACG80T_002147"
     CDS             9938..>10820
                     /locus_tag="ACG80T_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4841777.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKN"
BASE COUNT         3294 a         2190 c         1756 g         3580 t
ORIGIN      
        1 atttgcatac ttcataataa taataacata atacctaacc tctcgtaaaa atactagatt
       61 tgttttcttt tatttttcat cactagatac attctataac ttaccgtttc gatttcgaat
      121 tcaaacattt ttgagaagca ttctcaatta aattctgcta tagtgtgaaa agggaggaat
      181 ttttatggaa atctatgaca ttacaatcat cggtgcaggt cctgtaggga tgtttgccgc
      241 tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt gacagtctac ctcaacttgg
      301 tggtcaatta gcaactttgt atcccgaaaa atatatttac gatattcctg ggtatccagc
      361 aataaaagca agtgaattaa tcgatcaatt agaaaaacaa ttaactactt ttaaccatac
      421 attccattta aaagaagaag ttctctcgct tactcgtgaa gacgaagtta tcgagattac
      481 tacaaataaa ggcatccatt attccaaagc agtcatttta gctttaggaa atggttcatt
      541 ccagccaaga aaattgaatt tagataatgc tgaatcattt gaaaaccacg gattggatta
      601 tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc gctattgctg gaggtggtga
      661 ttctgctatc gattggacgt tgatgttaga gcctattgca agcgaagttt acttgattca
      721 tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca cgcttgaaat cttcatctgt
      781 caacttgctt actccctatc tcatcgatgg attatcaggc aataatgggg aactgacaga
      841 tatccggtta aaaaaagtga aaagtgacga gacgattgat ttgatgattg attctctgat
      901 cgtcaattat ggattctctt ctaatttaga acatctttct tcttggggac ttgatagtac
      961 cagaaatgcc attactgtaa aatcagatat gtctacttct attccaggcg tttatgcagc
     1021 tggtgacatc tgtacctatg aaggaaaagt gaaactgatt gctactgggc tcggtgaagc
     1081 acctactgca gtgaacaacg ctttacacta tattaatcca aaggaacgaa ctcagcctgg
     1141 tcacagcaca agcttatacg ataaaaatct tcgtcctagt tgatttttta tatctctttg
     1201 catttcaagt taaacccgaa agcagtttct tgcatttttt tcatgttccg ttaacatgaa
     1261 atagagaaag aaaaaataaa gggggagcaa taatatggtc gtcaaaactg agactttaga
     1321 agaaaaggca agagaactat tgaaggaacg tggcgtaact gtagaagata tcgcacaact
     1381 tgtcatgttt ttacaaaaag actatatttc tgatttgact ttggcggatt gtactgaaag
     1441 tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc attatcactg gaatccaatt
     1501 agatatatta gcagaaaaga aaaaattgat gagtccgttg caacatatta tcgaagatga
     1561 cgaaggcctt tacggtatcg atgagatcct agccttgtca atcgtgaatg tttacggtac
     1621 gatcggcttt acgaattatg gttatatcga caaggtgaaa cctggtattt taaaagaact
     1681 aaacagccac gatggcgttc atgtccatac cttcttagat gatatcgtcg gagcgatcgc
     1741 cgctgctgct gccagtcgat tagcacacaa agaaccagaa aaaagaagtc gccttactca
     1801 ataattcaac aaaaaaagaa agaaaacgtc tatttagacg agttctttct tttttgtttg
     1861 tttcaagcta ttttttccta agaaaagaaa aacagcaaaa aataattcta ataatacaaa
     1921 aaataagata aagctatgca tgaaaaattc ttctggaagt acattgatga ttggatcagt
     1981 aacgggatcg aataaccagt catcgttgtt gaaaaacgtt tcatgaaaaa ggataaaaaa
     2041 gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact ggcagtaaca tgccgatttg
     2101 aaacggacga atcaaccgcc aaagccgatc attcttttgc aaatatttga taaacaaaat
     2161 actcgggatc aaagtgatga aaaacacagc ataatccact aaaaacaaaa ttttcacttc
     2221 ataaaagtga tgggctccgc tagcagaaac tgggaaatct ggcaagctga ggatggtttt
     2281 gaagggatta ttgagataat tcattaggtg atcaaaattt ttcagtaacg tctcttgatc
     2341 taaagaagta taatccaaaa tttgcaaatg tccgatatca aatacgtata gtggtcgaaa
     2401 gttgatcgtc acaccaactg ccagactgat cagtgtcaga aataggctga acaatccagc
     2461 atattccatc cactgccatc tcgaattttt catattagaa gctccattca tccagtgatt
     2521 gtttcacata ggtagctggg ataggcaacc caggtacatc ttcttctttt gtaaaacctg
     2581 ataaaacgag caaagtatct atattattgc gtatcccagc ttggatatct gtttcatagt
     2641 tatctccaac catgattact tcttcttttt gcagccctaa tacttcaacg gctttatcca
     2701 taatgatggc ttctggtttt ccgatgtaga tcggcggcgt ttgggttgct gtctctacaa
     2761 aagagatgac tgaccctgct ccaggaagaa ggccgcgttc agtaggtata ttcttatctg
     2821 ggttcgttcc aatgaaagta gcaccttttt gaatagcaag tgttgcttta actacttttt
     2881 cataggtcag ataattatct aagcctacca ctacatagtc aggagcctct tcttcccatg
     2941 taaatccggc agctaaaatc aagtcaatca atcctgcttc tccgataaca tagacttttt
     3001 tcccttttcc atctgctttc ataaaatcga ttgttgccaa agtagctgtg tagactgtct
     3061 ctggagctac atgaatgtca aactcatctg ctagacgatt agctactgtt tctggtgact
     3121 tagtcgtgtt attggtaaca aacaagaaag gtaattttct tttttgcaat tcttccacaa
     3181 atcgtttacc ggccggtatg ggttgtgtcc cacgataaat cgttccgtct aaatcaatta
     3241 aatatccttt atagtccatt catccactac tcttcccttt ttctgatcgt aaatgctgtt
     3301 tttgtttttt ctacttgttt aggttgttct tgttttttta ctttttcaga ggtttttgct
     3361 gactctgttt ttcgattttt aataaccggt tttttgcgat tagatgcgac tggtcctttt
     3421 ttctcatcga tatacgcacg attatggttt tttttctttt ttcggcgctg agttgtcttt
     3481 tcttttttac cgccgattct ctcgatgaca aaatacgcac aaccaaaatt gcaatattcg
     3541 taaagatagt cttccaagga atcgattcgc tgatctggat gcgcccgtcg attatccgct
     3601 tggaaaaatc cttttaggcg tagttgttca taaccccaat ctcctacgat ataatcatat
     3661 cgggctagta catcactgta acgttctcca agcttttctg cattaaatcc ttcacgataa
     3721 tcggacacaa gccgataatg tctttcacct atcaaaaaat tactgtcatc taacagtgtc
     3781 actagctcgc ctttgacttt ttctgcttta ggctcctcaa cgatttcttc caaagcagct
     3841 gtcagttctt cggtgactgt tgtttcctgt tcagctgatg attttttctt agcaatttct
     3901 gttttatgct caacgttctg ctgttcattt tgttttgcag acatgcgttc accacctatc
     3961 ttttttatta taccttgttt ttatttgatt gggtagtgag ctttcaagta atctcctacc
     4021 acacttcgga taaattcagg gaacaaaaat tcattttttc cagcaatctc aatcgttgga
     4081 aaaaatggaa caaacatcaa atgatctacg gtagtaaaag tataattttt attcagatcg
     4141 attggcttgt tcttccaaaa gacttgatgg ttaatagaat catatgtgat tccattatag
     4201 accatctgcc caaaaatctt accgcgaaat cccattccct tcataggata gttacgtaaa
     4261 aagttacgat ttttttctat ttctaaaact aagcggatca gatcatttcc acccaatgtg
     4321 acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa gctgatcctg attgacccgc
     4381 ccttttggaa tttcagttaa aaacagacca ctgtttaata ttgctgcttc cgtctttcct
     4441 cgttctttca ccgcctcaaa ggctacttgg ataaacgaat gctcattgaa gatatctaga
     4501 ctcagagaaa atggcaaatc tgctacttct ttttctttta ataattcatg cccacgttct
     4561 agataactat ttatttcttg ttcatcttct gtaaaagctg tcattgtttc ggtagggatg
     4621 gctttggctg aatgcttaag gatttttttc tcttcatcga tggttaaatg gacttcaccg
     4681 acatactgcc cgtactttcc agcggctgct atttggacat cattgattac gtgaccttct
     4741 ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa ttgctggtat ttcttttgca
     4801 atttggaaat catcctgaat cccgagatga ctcatcaaaa ctaaaacatc cacttgtgaa
     4861 cgtaaatctt cgactaattc tggcaaaata tcataaggat tccgtatgtc ccagccattc
     4921 ggattataag taagcggaaa tggagcagta aatgcaatta gtccgatttt cgtctggtgt
     4981 ttcgttgtaa tgattttata tggtttcgtc catgccggtg gctggagcgt atttttatcg
     5041 aataagttat ccaggatgat atcaaactca gcatgatcgt ataaatgatt caactcttct
     5101 ttggaatttc ctacgccttc attgtttcct atcgtcgctg catcataacc aacttcattc
     5161 atcagcaaga tattttcctg tccattcgtt gcttctgaca atggatgcca acgatccaca
     5221 aaatcaccta gatctactgc aatcgttgtc gttttttctt tcttttcgtt ttctcgttta
     5281 cgttgatcca aaaaccttct gatccttggc caattttcta aatgagaatg cagatcattc
     5341 gtatgtaaaa tcactatttt ttccatgtac cctctcgcct ctctttatct ttatcatacc
     5401 ataggcagct ttttcttcca ttgaaacaag ctctatttca gagtttttcc tgccttgaag
     5461 caaattgtat aagtacaata ttcgataaat cagtctggct aaaaaatcag gaacattcat
     5521 ttttacagta ctataaaaat aaaaaaaaca gacccgaaag tctgttgtcg tttcattata
     5581 atcagacaca tggcggaacc tgcttagtgc tgcttccttc cagacctgac acgcttcaca
     5641 agcccaccat tgtcctagcc tttcggcaaa gaatagtata ccttattttt cattcgttga
     5701 ccagtctttt tttaatttct tttttcgctg gcggagcttt cggaaaaagt cagtcaaaat
     5761 catcccacat tcgtcttcta aaatgccacc ttctacatat gcttggtggt taaaccgctc
     5821 gtcctccaaa agattcatca aggtaccggc tgttccgcct tttggatcat acgcgccgaa
     5881 ataaacttct gctacacgag acaaaatcat cgcgccgctg cacataggac aaggctctaa
     5941 ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc actttttcac aggcttcctt
     6001 gattgcaagc atttctgcat gtgtagtagc atcttgcgtg gtttctctaa ggttatatcc
     6061 tcttccaatc acttttccat ccaatacaac cactgctcca atcggaactt catgaagcat
     6121 ttctgctttc tttgcttctg ccaatgcaag gcgcatccat ttttcttttt ctactgcatc
     6181 aagatttgta tttaccaatt acccttccct gcctttgttt tttcttgttt tactttagca
     6241 tatgataaaa aggctgtaac taactttttt ttctatggta aaataaatac aagaggtgaa
     6301 caagatgaaa atggataaat tacttgctct ttacccacaa gcacagaaaa aaaactctcc
     6361 agcatcagaa gcggatatcc tttcttttgc aattgatgat gcttttatct ggatcaaaca
     6421 agattcatta tctcaacaag aaaccagcct tttgaaagcg ttattccctg taatcaacga
     6481 tcaaaaaaaa cacccatggt atcgctatct ttttaaagat gcttcttgtg ttcaagaaaa
     6541 aagctttcgt ttgatccagc ttcacgtcga atcaaaagga gaattcttaa aaaaagaatg
     6601 gcaagatact attttggaat tatttcgcta ttctgctgat ttcttttttt atacagacac
     6661 agatgcgctc ttagtcgaac aaataaaccg tgattatctt gatggtactg aaatcaacgg
     6721 tatttttcta tctttagatg ctgattttga tacgacaacg tctgtattcg ttggttcttt
     6781 ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa gaaagaaaaa tttttttaac
     6841 agagcaaaac aatctttata ctcacagccg tactttttct atgcaagatg tggctttaca
     6901 ttactataca aaagatactg taaaagatag taagttgatc aaaagttaca tagaactgat
     6961 caaacaaaat gaaatggaag tgatcattca agaattatgg aatgaattag gcaatgtcag
     7021 ctctgctgca aaaacactgt ttcttcatcg taatactcta aaatatcgaa tcgaaaaatt
     7081 tcaagaacag tctggtttca atctaaaaaa agccaacgat ttactgtttt gctatctttt
     7141 attgcttcat gaacataatt aaagatagat taaaggggtt gtgacaataa ttttgtcaca
     7201 accccttatt tcgaatgaat agtgttcaaa agctagtttc tcttacatca ttttttcaac
     7261 aaaatgcatt agccggtctt ttacatcttc ggttgaatct ttgattgaat caccataaac
     7321 agagattttc ttttttcctg atttgatttt tgctaacatt cccatcaaag aatccaagtc
     7381 actatctgaa agaccatcta cgatttctag taatttttca tttccatcaa atttgtcata
     7441 gacgaatttt tttactttcg tccgagtagt catatgatgg atcttttcca ttacttttcc
     7501 cgatacaacg actgctgtcg ctacactggc agcggctgct acgcttaatc cgattgttac
     7561 ttttgttgat gttttcataa gtatcagctc ctttttatct atctccatca tagcatagac
     7621 aaaaaaataa acgaactaat ccactcagtg aaatctcaat cctttaggga aaaagttctt
     7681 tactgttcct tttacttgtt tcccaaatcc gacaggtatc ttatctaata ctagcaaaac
     7741 ccatccttga ttcccatcac gttgaaatgt ttctcctgct gtgtaagatt gccattcctt
     7801 ttgagtgatc ggcaagcaag gtatattctc tatctttttt gtagcaagcg ctaatgcata
     7861 gctaggttca aatcgattct ttttgaaatc tcctaaatgc aaacctgtac gtacgacttt
     7921 taaaccatcg agagaagggg ctaactcagg aacttcccaa agatggtcgt tgaacactaa
     7981 aagtcttcca gtagcttcat aatgaaaatc atttgaaaac tccgtccaga gtttttcctg
     8041 ctcttttgtc atttgcactt tacttttttt tcgtgtcttc ttttctttat gcatctgatt
     8101 ttgtccatga aaagtcagct ttgcaacaaa atgtccttca ccctgatctt tatgaggcca
     8161 tatacgtatt gttttttcca aaccagctac gcttccccat tcagaacgtc ctgaactaac
     8221 ggattgagtc aaaggaatct cctcaatcgt tactggataa ttttctacaa gccagctaat
     8281 gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa taaatcagtt gtcctttatt
     8341 ttttagcatt tttattgctg aagacaaaat ctcttgctga cgtttttgac agtatagcgg
     8401 agattcttct gtccactctt ttattgcatt tggatctttt cggaacattc cttctcctga
     8461 acaaggagca tcaaccacga tccgatcaaa aaagcccgaa aaatgaggga caagttctgc
     8521 gggtgcatga tttgtcacta ttgcgttcga aactccccat cgttctatat tttctgataa
     8581 aatcttcgca cgtttcggaa agatctcatt cgtcactaat aaccctttcc ccttcatttg
     8641 agcagctaat tgggttgatt taccaccggg agccgcacac aaatccaaaa ctttttcacc
     8701 gggttttgct gcagcagctg ttccaacgat cattgcactt ggttcttggc tatattcata
     8761 cccagcttga tggaggaaag actttccatt gacagtgcca agaaatccct cattgctgta
     8821 gggagcgggt tgtaattctt cgctatggta agtttggacc atgtccaatc ctgccggttt
     8881 tagtggattc caccgaaatc cttttttcac gcttccttgt tcaagcgcag aaaaaaaatc
     8941 ggatgcttct tcgcctaaaa gaagacgata ttttttgata aattgctgtg gtaacgttgt
     9001 cgcttcctcc ttcattccaa tgtttcgtta gacattttac aatctttttt acgaaaaata
     9061 aaggtggctg atctactttt tcatgttgct gcctaaggtg actgttcctt taagatattt
     9121 tccaagtatt catccgtcac ctcgttttta tcccaaacag cgttcatcat ttcaagaaaa
     9181 tcttgagaag aatcatctga gcgcaaactt cccaatgcat taagccatac caattcaagc
     9241 gacttttctt ctttgatctt cgtgtgacct ttgacatatg agaaaaatgg ccgcttaaat
     9301 agcgaatcca tgtctaaatc agattttgca atgatttcac gaatactttc atggatagaa
     9361 aaaaaatctt tcaataagta gacaatgctt aaaacatcat cggaactgca ttcagctaag
     9421 tagttttcaa aataaacagt gatttcttct aatatcttct cttttaaccc gctgattgat
     9481 ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga actctgctaa ctctcttgca
     9541 gtaattgact tcattccata ttcttccacg tataccatac ttttgaacag caaatattct
     9601 ttactataat ttgttactct ccccatcatt cttacctccg tctcttttca tctacccata
     9661 aataaataac ataaataatt ctaaaaatat tattgataat tatcaaaaca tatttagtta
     9721 tatccattaa aaatcaattt ttccgcctta aaaacaagaa tatcttgaca aaatacccta
     9781 ggaaataaac aatagaataa acaaggtaac aaaaaaatta aataacgtta tataaaaaag
     9841 aaggcgacac tctgtgagaa taacacagac tgaaatgact ttccactcac agtttttgtc
     9901 taactatata caaaaacaga ataaaaagga ggtatttatg ctaattgaag caagaaaaaa
     9961 agagttactt gttctacaaa tggtcattca aaaaaataat tgttctttac aagaattatc
    10021 caatgactta catattccaa aacggacaat caaagaactg atccgaaaaa taaatgtatc
    10081 actcaagcaa tttttattga ttgatgaatt tatttattct aatcataaag gagagatcca
    10141 aaccaaagaa accgatcaaa ctaaaaagtt agctgttttt tctaagttaa aattatgcta
    10201 cctaaaagaa tccaatcgtt ttaactattt gatgttacta atcaattatc cacaaactgc
    10261 tgtaccaaaa aaatatttac ttgaacaact ttatatatcg ccctcctatc tcgaaaaact
    10321 gacaaaacag ctcaatcatt ccttaaaaaa attccattta cggatcctat ctgtaaaaaa
    10381 ttgctgtact cttgaaggaa atgagttatc tatccgtctc tatctttact tttttctttc
    10441 agatacattt ggaggtaacg attggccatt ttcctcactt gaattaaatg cactaaaaat
    10501 gaacagtaca gatgagcaaa tagaaaaaat ttcaaaacaa gaaaaaaata tctacttgct
    10561 gttaacttta ttcatattac gaaacaaaca acaggcatat tttccaaaaa cttcttctga
    10621 aatcactgaa ttgatggact tattgcagca tacacatgat tattcagaat actttacttc
    10681 tttttcagtg agtcgccctt gtgaagagac agaaacagag agagcctact ttaattattt
    10741 tttctattgt ttttttccaa atcaaatctc ttttcgacaa aaacaggaga ttggtttttt
    10801 attttccaca agcaaaaacc
//
LOCUS       DAZUKC010000079        10759 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000079 DAZUKC010000000
VERSION     DAZUKC010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.081"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(304..1152)
                     /locus_tag="ACG80T_002148"
     CDS             complement(304..1152)
                     /locus_tag="ACG80T_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4841778.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1316..2218
                     /gene="gnd"
                     /locus_tag="ACG80T_002149"
     CDS             1316..2218
                     /gene="gnd"
                     /locus_tag="ACG80T_002149"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFS4841779.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKRTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2220..3221
                     /locus_tag="ACG80T_002150"
     CDS             2220..3221
                     /locus_tag="ACG80T_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFS4841780.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3218..3502
                     /locus_tag="ACG80T_002151"
     CDS             3218..3502
                     /locus_tag="ACG80T_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFS4841781.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3535..4332
                     /locus_tag="ACG80T_002152"
     CDS             3535..4332
                     /locus_tag="ACG80T_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS4841782.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4346..5179
                     /locus_tag="ACG80T_002153"
     CDS             4346..5179
                     /locus_tag="ACG80T_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS4841783.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5197..6054
                     /locus_tag="ACG80T_002154"
     CDS             5197..6054
                     /locus_tag="ACG80T_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4841784.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6076..6585
                     /locus_tag="ACG80T_002155"
     CDS             6076..6585
                     /locus_tag="ACG80T_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFS4841785.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6622..7023
                     /locus_tag="ACG80T_002156"
     CDS             6622..7023
                     /locus_tag="ACG80T_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841786.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7135..7488
                     /locus_tag="ACG80T_002157"
     CDS             7135..7488
                     /locus_tag="ACG80T_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841787.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVEADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7522..7965
                     /locus_tag="ACG80T_002158"
     CDS             7522..7965
                     /locus_tag="ACG80T_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4841788.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7982..9067
                     /gene="uxuA"
                     /locus_tag="ACG80T_002159"
     CDS             7982..9067
                     /gene="uxuA"
                     /locus_tag="ACG80T_002159"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS4841789.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            9060..9773
                     /locus_tag="ACG80T_002160"
     CDS             9060..9773
                     /locus_tag="ACG80T_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4841790.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(9980..10129)
                     /locus_tag="ACG80T_002161"
     CDS             complement(9980..10129)
                     /locus_tag="ACG80T_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841791.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            10525..10725
                     /locus_tag="ACG80T_002162"
     CDS             10525..10725
                     /locus_tag="ACG80T_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4841792.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3607 a         1698 c         2319 g         3135 t
ORIGIN      
        1 agtgttatca aggaaatact taattcacca aaattttttt taatttttta gaaaataaga
       61 aacaaaaata ataaaaaaaa gaaaagataa attttttact actgtatact aactttgtta
      121 tcgcatctaa caagtaactc gaataatacc aatacaacct tctccaaaga tgcgatgaca
      181 tacgttctaa cccaccgact attgatgttc tgtaccaccc ttgcagaacc aaaaaagcca
      241 ctaccaaata tttggtagtg gctttttttg ttttatggat ttttcgcttt ttctgttatg
      301 gtgctattct ttgatttcag aaatagtatg tcgaacttta gcgatagaac gacgtgattc
      361 tttttcgttg taaaacatca aaatcacata tagagagtcc aaaatgctta gttgagaaat
      421 acgggaagct aaagcttcag atcggaattc tgtttcttca gaaatagaaa taaaaacgac
      481 atcgccaagt ttagcaaggg aagaattagc ttggctagta ataacgattg tttttgctcc
      541 agtttttctt acggtttcag caatatggat tgtttctttt gattgccctg tgtgagaaat
      601 caaaacagca cagtcttttt ccgtcaataa agaagcttcc atcaattgaa tatggtaatc
      661 cgtgctgtga gaagttgcga taggggaacg caaaaattta tgataagcgt cagttgctac
      721 aatctctgaa ccgccaacac cgaaaaaata gacacgttta gattcattga taatagtagc
      781 cgcttttttt agatcctttt gttgtaacaa cttttttgtg tcatttaaag ttgaaatact
      841 agactcgaac accttttgcg ccatagtgag ctcagtatct gttttctgaa tattttcgtg
      901 gatagaaata gcagatagat cattttcttg gatcaacatg gccattttaa aatctttaaa
      961 tccattataa ccaagttttt ttgtaaattg aaaaaaagta gagtcagcaa tgcccagttc
     1021 acttgctaaa ttgctaatag gactatgact tactttgctt ggattttcta aaatataatc
     1081 ggcgatagcc tgttcttttg ggctgaagtc gttgtacatt gttttgattt gtagtcttat
     1141 ggagacgctc atgagaattc ctcattttca taataagatt atcatttttt ccgttctcat
     1201 taatgttaaa gatacaagga gagtaggaaa aaatcaaaca aaaaatattt ttgtaaaatc
     1261 tatttacaaa aaaatttttt tgtataatga gggtataaga aatggaggcg ctatcatgga
     1321 aattggtgta attggtttgg gaaaaatggg attgaattta gctttaaact taaaagagca
     1381 ccatcacaaa gtacaaggac tcgacatttc aagtacagct gttagtcagg caagagaaaa
     1441 tgacattcct gcttatcaag agatctctga atttctagct acttttgagc agaaaaaaat
     1501 catctggtta atgcttcctg ctggagagat aaccgaaaac atgttaagaa aactttttcc
     1561 aaaactcgga ccaggagaca ttattattga aggcggaaac tcttattata aagattcaat
     1621 ccgtcgagca gaagagtttc agaaaaatga aatcggttac ttggattgtg ggacttctgg
     1681 aggaatcgaa ggagcacgac atcaagcctg cttaatgatt ggaggagata aagaaacttt
     1741 tctatccgtg gaacaattat tcaaagatgt agcaattgaa agaggatatc tgtatgctgg
     1801 tgcatccgga agcggacact tcttgaaaat gatccataat ggaatcgaat atggaatgat
     1861 gcaagcaatc ggtgaaggat tccaacttgt acaaaaaagt gagtatgctt ttgacttgga
     1921 acaagtcgca cgtgtttgga atcatggttc ggtgattcgc ggctggctga tggaaatcat
     1981 ggaagatcag ttaaacgaac atcccaattt aagtaattat cggggaatcg tttctgcatc
     2041 aggtgaagca aaacggacgg ttgaaacagc gctagaaatg gctgttccag ttccagtaat
     2101 tgcaactagt ttgttcatga ggaatctttc acaggaagaa gatagttttt cagcaaaagt
     2161 agtttcagca ctaagaaatg ggtttggcgg acatgaaata gttgaaaagg gtgagtaaaa
     2221 tgccagatta tcatatagca atcgttaatt ccagcagttt cggaagagtc tttccacacc
     2281 acttggaaag attaaagtca gttggagacg ttcatttctt tactgttgat caagagattc
     2341 caggaaaaga actagcggag ttacttaaag gattcaatat cattattgca agtgttacgc
     2401 cattttttcg acgagatttt tttgaaaaca aagatgaact gctattgatt tcccgacatg
     2461 gtattggtta caacaatatt gatttaaaag cagcaaaaga acaccagaca atcgtatcta
     2521 tcatacctgc tttagttgaa cgaaatgctg tagcagaaaa taatataact aatttgttgt
     2581 ccatgcttcg acgcacgaca caagcacaag caagagtcaa ggacgatcga tgggaagatc
     2641 gggcggagtt cgtcggtagg acgctgttta acaaaacagt tggtgtgatt ggtgcaggaa
     2701 ataccggaag cggagtaatc gaaattttgc gtaacggttt tcgctgtgat gttctagctt
     2761 atgatcctta caaatcagat ttatacatcg aaagctttgg tgcaaaaaaa gtagaacttc
     2821 aagaattact ggaacaatca gatatcattt gtttatgtgc gaatttgaca gaagagaact
     2881 atcacatgat ttctgataaa gaagtcgatc agatgaaaga aaatgtttat ctatctaata
     2941 gtgcaagagg cgctctagtt gatgaagaag cgatgattaa tggtctgaaa agcggaaaga
     3001 tcgcaggata tgccacagat gttttagaag aagaaccggg acgtcaagat catccgtatt
     3061 tagcgtttga acaagtagtg attacgccac atatatcggc ctacacaagg gaatgtttag
     3121 aagcaatggg tgaaaaatgt gtcagagatg tcgaacagat cagtcagcac cttcttcctg
     3181 aacgttctgt ccagacaaag agcaactata taaaaggatg atgaagaaaa tggtgaacgg
     3241 tttaaaagtg aaaacaggcc ctcaatttta tttatatgaa gaaggtggta tttcaaaagt
     3301 ttctgatctc ttgaaatcat acggcgcgaa aagagtactg gtcacccacg gaacggtatc
     3361 atgggaaaag gcgctaccta aattagtatt tttgaatgac gaaacgattc aattttttta
     3421 tcatcgttat tctggagaat gtagttatgc tgaagccaga cgaatagcta caatcatcaa
     3481 aaaaatgaaa tcgatttcgt agttggcgtt ggcggaggaa aactgaccga tctagtgggt
     3541 tatagtgcca atcttgcaag aatcgatttc ggagtcatcc ctactttagc tagtaattgt
     3601 gctccatgga cacctcttgc tgtcatgtat aaggagaatg gacaagcaga agggaaaaca
     3661 gaacactttt acagacaagc tgcttttttt ataacagatc cccaattagt gattgattcc
     3721 cctgttccat atttcattgc cgggttagca gatacactag caaaatggta tgaatctgag
     3781 ctcatcttag aacaagattt tttgcaagat gaatcatttt tgaagctagc acaagatact
     3841 gcgaagatct gcaaagaaga gattttgaat cattctgcta aagcaatcca agatatggca
     3901 caaagaaaac tgacatcgga gttcaaacag ctttcagaga ttatctttgc tgttgctggt
     3961 ttagttggag gattaggaga taaatacgca agaaatgtgg cagcacatgc tatgcatgac
     4021 gctatcagta aatatattcc ggaaagccat cgttttctac atggagaaaa agtagcgtat
     4081 gggattttct atcaactggc actagaaggt cgctgggaaa aaatagacga gttatatcct
     4141 ttgtatcggg aattagaact gccggtttct ctagaacaaa tgggattatt tccgaaagat
     4201 aaacagataa tagataaaat ggctgaattt attgattcga aagagaaagt gcatttgatt
     4261 cctatagagg taacacagaa atcactgata cagtcaatga tggaactgga aggttatatc
     4321 agaaagatct gaaggaggag gacacatgga aacattaagt gtagtacaaa gtttgttgat
     4381 tgctttatgg gtagccgcgg ttatgtcccg ctggttaggt ggaggagcca cgttgacctt
     4441 acgtttttct cctttgatga ctggattagt cgtcgggttg atcatggggg aagttgcaca
     4501 agcgatgatt gttacagccg ctttgcaaat gatttatatg ggggtttttt ctccaggagg
     4561 atcaatgcca gctgaaccat ctattgcagc agctatcgca gtaccagtcg cattattagg
     4621 ggatctgtcg ccagaagcag cgattgccgt tgcggtgcca gtcggattgc tcggaagtta
     4681 cttgtatcag tttcgttttt tcatcaatac ttttttaggt aaatatacag acaaagctgt
     4741 agaagatttg aatacaaaag ctatggcccg atcaattatt ctttatccaa cggttgcctc
     4801 atttttatta ttcgttccgc ttgttttcat tgctttatat ttaggggcgc cattgatcgc
     4861 tgatgtaatc aaagctttag agggaacagt cgttatccac attcttgaag tagtaggtgg
     4921 agggttagcg gcaatcggta tcgcgacaac tgtatacgtt atcggtcgaa aagattatct
     4981 ggtctttttc ttccttgctt attttatgag tgttgttttg aaatctttag atattacgat
     5041 ggttacttat gccatttttg ggattattat cgctttgatc tttgtccagt cgcaaaaagg
     5101 gaaacaagta cagcctgcca caagcaataa caattcagca tcttttgatg acgaagatga
     5161 ctatgatgac ggattttaat ttaggaggaa tgggaaatgg ctgaaataat ggataatcaa
     5221 acaggtgctc ctgaagaaat cacgaaaaaa gatgtaacaa aagcctatct gagatggcat
     5281 tttgcaaacg aaattcctca ctcttttgaa cgttacttgg cgccatcact actatacgga
     5341 atgatgccaa tcttgaaaaa actttataaa gatgaagact ctttaaaagc agcatataaa
     5401 cggcagttac tgttcttcaa cactcaatta agctggggtg gcggtgtgat tactggccta
     5461 atgtcttcta tggaacagca aagagcagaa gaagagtatg aagaaaaaga gatcttgatg
     5521 caggatgact tgatgtataa cacgaaagcg ggattgatgg gggcactagc aggaatcgga
     5581 gatgcaatcg attcaggaac cgtccaatat atttttatcg caatcgcggt accttgggca
     5641 caacagggaa gtcctttagg cgcacttttt ccttttatct gctttgggtt gtatcaggtt
     5701 ttactagggg tatttttcgc aagacaagct ttcactatgg gccgtaatgc tacaggccta
     5761 aagcaaaaca ctggtgttca gacagcgatt gaaatgctat cagttttagg gttgtttatg
     5821 atgggggtgc ttgcgggaaa ctatgtaaaa gtagaatctt cattgaaatt tgcgatatcc
     5881 ggacgtgagt ttgttatcca ggaaattctt gatcaaatcg tgccagggat attaccttta
     5941 gctgtcgtaa tgggtgtgta cttgttctat acgaaaaaag gattgaaagt cacacaagca
     6001 ttattatggc tgactggaat attaattgtg ctagcaggaa tcggtatttt ataaaaagaa
     6061 gggaacgtga ataagatggg tgtagtcaat ttagcgcgtg tggatgagcg tttgatccat
     6121 gggcaagtaa tggtaacgtt atctcaaaaa agtggagtga attccatttt tgttgtagac
     6181 gaagttgtag caaaagataa atttatgaga gatctttaca aaaatgcagg aagccgtaca
     6241 ggacaaaaaa caattgtcat tacaccagag aaagctaaat tctattggga tgaatatcaa
     6301 tttaaagaat acaattgtat tttgatcgca aaaacagtcc aaacgattta cgacttagta
     6361 aaacacggaa tccccatgga agaattgaat attggaggga ttgctcaaaa aaatcctgat
     6421 acagatcttt tagtgacgaa atctgtttat ttaaataaat cagatgctga aaaattgaaa
     6481 gaaatgaatg aacaatatgg cgtaaagaat atttattttc aagccactcc atctgcacct
     6541 aaaacaggat taaaagatgt attgaacaag tttgatttgt cataaacaaa tgcttagaaa
     6601 aaagataaag agggcgaaaa aatgaagaag aacgagctgt ttcgtgattg ggaatttcgt
     6661 tatcgctata tttaccgtaa gcgccgtaca aaaaagagta aacagcgttt tctttcagca
     6721 ttagtatcag atatttacag tatgagaaca gatgtgacag tcattgctta cgatacgcta
     6781 gcctaccgat caaaaaatat ttatgttgga gatatcgaaa aagcagagaa agttatctgt
     6841 acttattacg atacacccgt tcatgcatta ggaagttatt tcatgtttga ttggaaagac
     6901 caaaggaaaa aaacaatcta ctctatatta ttgtccttca ttttgttgtt cagtttaggt
     6961 tggtggggaa tgatgatata taacaaaaat cctcatcacg tattcgatct gctatccgta
     7021 taaacaggta tcactgtact agcatttggg agctactttt tcttattggg aaaagcagct
     7081 cgcggatggc ctagcagaca aacttttatt cgaaatactt catccatatt aacgatgcta
     7141 gaaatgatcc gtacgattga tgatccgagt gtagcgtatg cgtttgttga tgaaggctgt
     7201 tatggagaaa agggcttaga cagcgtgaga tcgggtatga aaaaagaggc tattcttttt
     7261 tatctagact ctgttgaggc agatacaccg ctacagtttt cgggaaatta tttttcaaat
     7321 aaagaacagt ggctgaaaca agttgataag ttgaaagaaa aaaacgtcaa ttatattttt
     7381 agtgcaagaa aaaagcaagc gcagttcttc tatttgacca aaacagattt acgagggaaa
     7441 acatttaatt ggcaaaatgc caatcagatt attgctcttt ttcgataagt aaaaagtgca
     7501 aaacaaggag tgagaagact aatgttggaa atcatgatcg ttacacatgg taagttgagc
     7561 gaaggactga aagatgcggc ggaagtaatt gtcgggacat cgagccatat tgaaacaatg
     7621 tgcttagagt ctggggagga catccaaaca ttcggagaga aggtcaaaga aaaattaact
     7681 atgatcaaca cagcagaacc catgctagta tttgttgatc taattagcgc aagtccgtac
     7741 aatcaagcgc tcttggcagt caatgaactg actgaagaaa agaaagcagc cacttatgtc
     7801 atagggggcg tgaatctgcc aatgttgtta gaagcaatca atcatcagat gcttcacaca
     7861 ccgatagaag aagcggtgga acaaattgtc aaacaaggaa aagataattt aactgtttgg
     7921 catgcttcag cggaagaaaa aaaattagat gaggacgatt tttagaagga aggctgaaaa
     7981 gatgaagtgg ggatttagat ggtatggtga aaaaggggat tcgatcccat taaaatatgt
     8041 tcgccaaata ccgggaataa atgggataat tggaactttg ctgactaaaa tgcctggaga
     8101 tgtttgggag atttcagaaa ttgaggagtt gaaagcatct gttgagagcc aaggactgga
     8161 actgcttgga attgaaagtg tagcgatcca cgatgcaatc aaggcaggga cagatgaacg
     8221 agattactat attgataact atatacgaac aatcaaaaac ttgtctcgat gtggggttcg
     8281 attgatttgt tatagcttca aaccaatttt tggatgggcg aaaactgatt tgtttaatga
     8341 aaacaacgat ggaagctatt ctcttttata cgatcagaaa atcgtcgatc agatggagcc
     8401 aggagaaatg tatcaattga tacacagcca gtcaaaggga ttcaaattgc cgggttggga
     8461 agaagaacgg ttgaaaaaat tcgctcagct ggaaaaactg tatgaaggaa tgaccgaaga
     8521 agaattattt gaaaatctga cttatttttt agaaaaagtg attccagtgt gcgaaaaagc
     8581 agatgtaaaa atggctatcc atccagatga tccgccatgg gaactttttg gttttcctag
     8641 agtgacgaaa aatcttggcg acttgaagaa aattctaaat gcagtagatt ctccatataa
     8701 tggactaacg ctgtgcacag gatctttagg ggcaaatccg gaaaacgatt tagttgaaat
     8761 catccatgag gtaggttcgc gcatccattt tgtccacttc agaaacgtgg agtttttagg
     8821 agaaagaaag ttcaaagaaa cggcacatcc ctctacagaa ggctcccttg atatgtatca
     8881 tattatgaaa gcgttggtgc aagaagggtt cgacggtcct atacgccctg atcatggtag
     8941 aaccgtgtgg ggtgaagtgg ctatgcctgg atatggcctg tatgatcgtg caatgggact
     9001 cacttacatg caaggtctat atgaggcgat aagtaaggaa aaaccactgg gagagcatta
     9061 tgaataaaat caagctcttc atctttgata tggatggcct tttattagaa actggacgtt
     9121 tagcttatcg tgcttatgta aaatcagcac aaaagtatga ttatgaaatg cgtaaggaag
     9181 tctattattt actaactggc caaacagaaa tggcgataag agaacaaatg ggcattttgt
     9241 acggagaaga tgttccatat atcaaatggc gagaagcaat caatcaatat aaagaaaaaa
     9301 tcgtgaaaga agataagaga gtatatacga aaaaaggagc agaagaaatt ttatcttttg
     9361 caaaagaaag aggaatccat acaatcgtgg cttcgtctaa tacacgggta aaagtagaaa
     9421 tgtatttaaa aatggaaaat ctatatgcct tgtttgatca aatcatttca ggggatgatg
     9481 taaaaaaagg aaagccggaa ccagagatct ttttgaaagc ttgttcaaaa atgaatattc
     9541 ctccaagtga agcacttgta tttgaagact cgattgcagg gatcgaagca gcaaggcgag
     9601 cgggcatttt gtcttttctc atcaaagatc atttgatagg gttgcccgat cataatggga
     9661 agcataagtt gaaagtagat gtaagtttat atgaatccaa taaagcagac tatgaatttt
     9721 atgacctgca tcaagcaaaa cgatttttag aaagtaaagg gttgtatctc taataaagag
     9781 gactattttc tagtcctctt tatttattgt cctgcacgac tgagggaaca gatgaaaaag
     9841 aagtaatttt ttattttgaa gtatctacct tatttgtagt gaacaattta gaacaaacac
     9901 aaaacgcatc tactgttatc taccagtaga tgcgtttaaa gagaagaacg agaatcgtcc
     9961 atatgagggt ttccactcct tatgcagatt tttttaattc ttgcactgct tgttcgatcg
    10021 taacacctgt cgctacatga ttggtatttt ctttgtcaaa aacgacaaat agattcaaat
    10081 ctgtatccaa ggttactcga tattgtatat ctttgttata gaatgtcata gccattctcc
    10141 tttctttaaa tacttgctga aaggcaacga acgcgttgat aacgaaattc catttataag
    10201 tataccacca attgaaaggt tttcatagtt aagaaatttt aaagtcaaaa gttgcagata
    10261 aaagcaaata aacaagaaaa caaaaagtga atcaatcgcg gttattcaac tttttcttcg
    10321 attcgaagac aatagaagac aatgaatttt atatttcatt gcattatcct tgtgttatta
    10381 gaaaaagcat gatatattaa atgagtaatc tattttgaaa cgtaatttga gcgatatatc
    10441 tacatagata aaactccttt tgcctatcaa atatttattg caacgaaaaa acgtacattg
    10501 atacgtatca ggaggaattt atttatgaat aacggtacag taaaatggtt caacgcagac
    10561 aaaggttttg gctttatcac tggtgaagat ggaaatgatg tatttgcaca tttttcagct
    10621 atccaaggtg acggcttcaa aactttagaa gaaggccaag cagtgtctta cgatgttgaa
    10681 gatggtcaac gtggccctca agcaacaaac attgttaaag cttaataaaa attgactttt
    10741 aaccgtcttt tcgaaagaa
//
LOCUS       DAZUKC010000080        10755 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000080 DAZUKC010000000
VERSION     DAZUKC010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.082"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            104..541
                     /locus_tag="ACG80T_002163"
     CDS             104..541
                     /locus_tag="ACG80T_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841793.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            636..1520
                     /locus_tag="ACG80T_002164"
     CDS             636..1520
                     /locus_tag="ACG80T_002164"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS4841794.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1744..4911
                     /locus_tag="ACG80T_002165"
     CDS             1744..4911
                     /locus_tag="ACG80T_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFS4841795.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            4979..5476
                     /locus_tag="ACG80T_002166"
     CDS             4979..5476
                     /locus_tag="ACG80T_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFS4841796.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            5495..5959
                     /locus_tag="ACG80T_002167"
                     /pseudo
     CDS             5495..5959
                     /locus_tag="ACG80T_002167"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            5956..6714
                     /locus_tag="ACG80T_002168"
     CDS             5956..6714
                     /locus_tag="ACG80T_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFS4841797.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(6800..7540)
                     /locus_tag="ACG80T_002169"
     CDS             complement(6800..7540)
                     /locus_tag="ACG80T_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFS4841798.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            7601..8854
                     /locus_tag="ACG80T_002170"
     CDS             7601..8854
                     /locus_tag="ACG80T_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFS4841799.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            9176..9403
                     /locus_tag="ACG80T_002171"
     CDS             9176..9403
                     /locus_tag="ACG80T_002171"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFS4841800.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            9526..9852
                     /locus_tag="ACG80T_002172"
     CDS             9526..9852
                     /locus_tag="ACG80T_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFS4841801.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            9920..10633
                     /locus_tag="ACG80T_002173"
     CDS             9920..10633
                     /locus_tag="ACG80T_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFS4841802.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
BASE COUNT         3667 a         1812 c         2245 g         3031 t
ORIGIN      
        1 ttttaaaaat tttttttact tagaatccag cgaccctaga tatttttctg tataatctta
       61 aataaagaaa acaatatcaa aaaatgcacg tagaagtaga taaatggagt ggttttgcaa
      121 atttctttta tttctagaag ttattcacgt atacggattt gtttttctca caaataaaaa
      181 ggcaggaagt acaatggatc atttgtatat aaaatatatt catcaatttt ccgttttggt
      241 gttaggcaca ctaattatag cggggattgt cgcatttatt attcaaggcc gtgacatatt
      301 aacagtacat caagagtttt tcagttatgc atttggtatt cctctgatcg ctataaggtc
      361 tgataacaaa aagtggaaga aacgctatta cattctcttg ctatttttgt gtttttatct
      421 gccacctaag atttttactt tattaggtat acaatgccta tcattttctt ttatttccta
      481 tatcattgtt ttaattttcc tcagtttcat agtatggatg ttttatcttg tcaaaaaata
      541 aatgaatgtt tcatgaacag tacttcatca cagcctaatt tttttcttta tgctatcgtt
      601 atattaaaga aatcaatcag aagggaaaga aagtgatgaa aaaagtttta ttagtggtaa
      661 accccagttc aggcggtgaa caagcaaagg aatttgagca attagcgata gcaaaattgg
      721 aatcggtgtt tgatgaagtg gtcgtcttgc atacaaaaaa agcaggggat gcaaaaaact
      781 ttactcgcga agcggctacg gaagggtatc atagtgtgtt tgtcatgggc ggagatggaa
      841 cagtcaacga aggaatcagt ggaatcgctg aacaagaaca tcggccaaat ttcggatttt
      901 tcccattggg tacggtgaat gacttggctc gagcattagg gataccgcta gaacctgagg
      961 aagcgatcaa tcatttttcg attgaatcag tcaagccatt ggatattgga aaaataaatg
     1021 acgactattt tatgaatgtc gtcgcaattg gctctatccc tgaatcaatc aatgatgtag
     1081 atgcggagaa gaagacgaaa tttggaaaac tagcttattt catgtcagga atcaaacagt
     1141 tagcttccac acaatcctac tcgtttcacg tagaagttga tggaaagaag gaagaaattg
     1201 agagcagtac cttgctgata ggactaacta actcagtcgg cggttttgaa acattacttc
     1261 caaatgcaaa agtggatgat ggtaagctcc atttggttta tttaaaagat tcttctttga
     1321 tcgatacgct taaaacattg ccagacctat taaaaggagt agatgaatct acgaaaaatc
     1381 tagcctacct tacttgtgag caattagttg tgtcattagc tgatgaggaa gaattgacga
     1441 cgaatgttga tggagatgaa ggggataaat tacctgtgac aatcaaagta ttgccctcac
     1501 atttacaggt ttattgttaa aatggaggtt gtgaccgagg agttggcggg agaacaccgc
     1561 ttgttcggga taagggactg tgacaagact tttgtcacag cctttttttt gtacaaaaaa
     1621 gaaatttaca cctaatagaa aaatttttcc ataaaaatac atattttaaa tatgaaagta
     1681 caattggaaa tagattaaaa cgataaaata aaattgtcaa aacgtcagag aggatggtgg
     1741 agaatgaatc aatgtgtaaa aaagctgatt gcgtcgattt tgtttgtctt ccctctcttg
     1801 atggtgacat ccgtttttgc cgatgtagag aaaacaaacg aatcaacgca atcagagaat
     1861 caaagttttg acaagactac tttttcagaa gaatcgatta cttctgaaac tacagaatct
     1921 caaagtgagg cttctactaa taaaaggcaa gaagaaaata agacaagtga gacctcagaa
     1981 gtagctgaac aaacgtctga tagcagtgaa gcggcactgg caagagctgt ttcagcggat
     2041 gggtacagcg aagctgccac aacagaagaa ctggcgcagc ttctcgcaga cgaatcagta
     2101 actaagatcc gactgattca gcctttgact ttagacaggg aattagagat caaacgagat
     2161 atcgttattg attttggtgg attcgctcac aatttcggca cacaccatat ttatatcaat
     2221 gagatacctc agtcgatcga gttccagaat tttaaaggaa cagctgcaca tccaggtgga
     2281 ttgacaccaa atatcgatgg aaatgccatt atcattgctt atatgaccga tttttgggga
     2341 agcagatatc attttacggg agaaataaaa tttaccggga cacttgattt gtatgatggg
     2401 tccaaattag gactgattta cgctcctcgt gcaactgtca cattagatgg tgtttccggt
     2461 gtgttggatg tccaaccagt gaaggaagga ctgaatgccg ctccgggtaa agcctatttt
     2521 tgtagaagct atcaattgaa tgtgatcaat ggtagtcaac tttatggacc gtatctaggg
     2581 aaattctacg gttttctaga ttcaggagat gagactggta gtggaaaaac agcgccagga
     2641 atccatatct taagcggttc aaaggtctct cttgattatg atcgggcaga tggcgttagc
     2701 gatgaaggag aggcagtcga tacgctacct ggtaatgtca tgtttgaagt cagtggttcg
     2761 ggaagcgaat tttcggtaaa tacaaagatc aatattacca atgacaacaa tcgcggaatc
     2821 atccaaatga gaggttcagg atcgaaagtc actgtatcga atggtgggaa aatcaccatc
     2881 aatacagcaa ctacgggcgg atttcgcttg caaggagatt ctagtcaaat ccatgtatca
     2941 agtggtggac aaatctcggt caaaatggct ggagatgacg atcaacccgg gaacaatggg
     3001 atgcgttttg taggaaaagg cctttctttg atcgtagaag gagccggatc agttatcgac
     3061 atcgataagc agagtgggcg ttccagcgca gttcgttttg agaatggtac gcaaaagctg
     3121 acagtaacca atggaggctc attgagggta agaaatacag gtgatggcaa atcttacgtg
     3181 aacagactca atcaaggaaa tcaagcgatc caattttatg acgcttctgg ttttctagaa
     3241 agtccaggtt cagctgattt tacagtcact ggagaaaaat caaatatcga tatacgcgca
     3301 gattccggag caactgtaga tacaactgga gatattgatt taaattttac ggcaggtgaa
     3361 aaaacttatt tagtcatggt cgggaaaaca ggggaagata agaccggcat catcagtggg
     3421 gatgtgttga atgtggactt agaatctcct acctattttg atttccaaaa caaacggatt
     3481 ggaagcgaga ccaatggcat aggcggctgg gtattccaag gaaatggaga ttcttcttta
     3541 aagatcgccc attcagaaat cgctttatgg cgaaaaacag gcgcaacatt cgataacttt
     3601 gaaaaagacc cggattattt ttctgatatg acagacttat cgatcaatgg gaccgactta
     3661 ggcaatttcg tatcaagccc tagtgcaggt ttaacaaatg aatttgctca gacaggtgcc
     3721 ggaatgaaaa attataatcg aatcagtgcc aataatcagc tcccgatgat tgaatcctta
     3781 agagtgccaa cgaatgcaga taaaaaaatc tatgggcatg tcgttgttcc cgaaggtgtc
     3841 gaggcaaagc caagagatgc ttggacaggc gaagtagaag tgactttacg tattacttac
     3901 gcagatacga cgaaatcatc tgtagaaatt actgctctca cgcaaggaca aacagatgaa
     3961 aatgaagggg caggatataa cccatgggga gaaggcgaac aaggcggctt gttcgaagtt
     4021 caagtaccca atgatgagag attaaaaacg gaggatcaag tgcaagttgt tgctgctaaa
     4081 aaagtaagag gtggccaaga agtgacagaa ctggactctg ccaaaacgac agtcgatgtg
     4141 attcctcctg aacctgctaa tttaagtacc acattgatca atgcagcttc tcgcacgatc
     4201 acaggtacag gtgaagcagg agccttggtt tcattgaaaa aaaatcaagc tgttttgtca
     4261 gaaacaacta ttgatgatac aggggaattt gttctttcta tcccagaaaa tcaacttttt
     4321 gcaggagatg tattagaaat tgtattaaac gaccaagcag gagaagctac tgctaagggt
     4381 gtgcagaaca agccaagtac caacgatgaa attgggaatc acaatccaag tgatacgcca
     4441 gttgtttatc atgatgcccc gccttttgca cctgctacga agatcgtagt agaaggtggt
     4501 ctctcatttc ttgccccttc caaactcgat tttggtcaga tcaaagcaac tggtttgaca
     4561 caagaagcat ttggccaaat ggatatacag gaaaaattat ggatttatga tcagcgagaa
     4621 acgaaaaatc cgtggatctt aacgataaaa ttatctcagc ctttcacaaa tgatgcagga
     4681 ttttctatgc aggatgtcct atatttcaag aaaggagagg agtatcaatt agtgaatgaa
     4741 gaagaactgg aaattgtcag aggacaaaat tcaacgaatg tgcaagcaga ttattcagaa
     4801 tatttaaata acggtcgggc gtttaaactt gtcttaacca aagaaaatca gatagctggt
     4861 aattactcag cagagataac atggacatta gcagatgcgc cgacagaata aagatcaagg
     4921 aggatgattg atgaatcgaa tatggaataa aagtcatcga aacaactgct ctttacttat
     4981 gatccttctc cttggattca tcctttggca gacacctgtc catgcctccg aattcaattt
     5041 tgcggtagat tttgaattac cggctaatca gattgataag aacaaaacat atataaatgt
     5101 aaggatggag cctgggaaaa cacaggaaat cccctacacg gtctttaacc gaacagataa
     5161 atcagtaaca gttgagacat tgatccactc cgctacgacg aattcaaacg gtgtgatcga
     5221 atatggagaa tcaaaaagta aatttgatga aacattaagt tatgatatga aaaacttagc
     5281 aaaaacagtg ggagaaataa ctgttcctcc tcaaagtgcg gtcactgaga cactttcact
     5341 gaaagcgcca gaaaaagagc tggctggtgt ggtcgcagga gggatcacgt ttcaagaaaa
     5401 aaaagagaac gtttctaaca aaagtacagt tggtgtcgtc aatcagtttt ctcaagtgaa
     5461 agcaatcttg atttgaaata gcgaaaaaga gtcttcccca gatttgaaat taaaaaaagc
     5521 ttatgcggat caaagcgatg gaagaaatgt gattaaggcg aatcttcaaa atccaaatgc
     5581 ttcttatatg ttcggcttga ctacatcggt tgaaatctac aaaaaagaaa caaaagagcc
     5641 ttacctttca ataaaaaaag aaaaaataga tgtagcaccc aatacaaatt ttgatttact
     5701 tgtcccatta gaaggaaaaa aattagctcc gggacattat caagcggttg tgaagggtgc
     5761 ttggaaagaa aaacgctgga atctccatac cgattttgaa ataactgatg atgcagcgga
     5821 ccaattgaat gaaaaggata ttgatttaaa aaatcagcca gaaacatttg actggcgatg
     5881 gctgatactt gttggagtca ttcttattgg cttatttgct ggattattta ttttaaaaga
     5941 aagaaggaaa aagaaatgaa aaaaacaatt gcattaagta ccgttctagc tggaagtcta
     6001 ttattatgta cacaaagtgt ttttgctgag aataagagct cagccactac agaaggaacg
     6061 atttctgtga ccaaaccaga tgctgcaaat cctgaagacg ttgttccagt tcctcctgtt
     6121 gatccaaatg atcctgatac tccttttaca cctgacgaac caaatcaggg aacatccgga
     6181 ttactgacga ttgaccaagc tccagatctt gattttggaa caatcaagtt aggtacagca
     6241 aaaacagtat atgctgcatt acaaagcgga acggacagta ctggagcaca aacacaagta
     6301 cagaatttcg tacaagtaac ggataaaagc ggaaattatg ccggttggaa attatcagtc
     6361 aaacgaacag aatttattgg tacaacatct gatacgaata aactaactgg ttcaaaggtg
     6421 aaattcaaaa acgccgtgat tcgaaatagc gcaactaata gcagtggaat gccatctacc
     6481 gtttctggaa ctggcgctga aggaatcgaa atcccagcag atcaggcagt agatctagta
     6541 actgcaggag caaatgaagg gattggcaca tggatttatt cattaggaga gaatgtggaa
     6601 caagggagac aatcagtgga attatttgtt cctgtatcca attatgctgt agacaactac
     6661 gtttcaacat tcacatggtc gttaacagat gctcctacag aagaggaagc gtaaaatcat
     6721 atcaacgatt tcagggagtg gagccgattc aaggcccact ccctgaaatc tgctatgctt
     6781 acgtctcagc tcttattcat caactcgaag cagttttttg gaagtcatgg aaacgttcct
     6841 ttgtcttttc aaagatcttt cgaccaacga gtaaggagtc accgttgaaa aattctaaca
     6901 cataatgagc agaatcaatt ttctgcaatt gcgataagtt gatacagtaa gattttaaaa
     6961 gtaaaaagta agcatacttt tctaactctg tcttcaattg ttttaaagaa gtattcacta
     7021 atttatcttc tgtagttgtt ttgaccataa cttggttttt caaacctttg atactttcaa
     7081 tatataaaat ttcccgagga tacaggacga gacttccacc tttttgatca ataaagattt
     7141 tatcgacatt tacgatctgc tgttctttcc aattgatctg ttctagcact tgacgaattt
     7201 gttcagataa attcctgtct tttctgatat atccaatcgg aaggattttc cgattgatga
     7261 tctctactgc aaaattcgta taagcggtga aaaaaacaat attactatgt gcattttttt
     7321 tacgtatttg ttcggcgaca tcaatacctg tgagtgattg cttcaagtcg atatcgataa
     7381 agaaaacatc gtgattatca atagtgccca actggatcaa ctgattgatt tgagagatag
     7441 aagtgagctg cacgatattc tcgatatgat gattgaccaa ttctctgatg atccattcgc
     7501 tgtgaaactg gttatcttct atgataaaat actttttcat atgcaggctc ctattaaaaa
     7561 aatatgtgta cattttgaaa caaaacaatg gaggaataag atggtcgcaa cagtgacact
     7621 ttatttgacc tttactttga ttttagattt aggtatctat ttattctttt cgaaacaact
     7681 gctagtaaaa aacagctttg gttgtatagc tttgatggtg gtgacacttt tacttcctat
     7741 ccctaaagtg agctttcctt acctagttgg cttattattg atgtttcttt tttattttac
     7801 tacgaaaaac ctgtttatca gctgtttgat cagtaatctt ttttttgcta tcactggact
     7861 cagctttttt ctggtttttg atctgatgga ttatttagga aagagacatc ttggaacagc
     7921 aagttttttt gcactactta tcgcatttat tttctatcta ttagtaaaaa gtatagacaa
     7981 acagacgaga tttctcaact tgctttttac ttatccaact gaacactatt ttggaggtat
     8041 ttgtatttta ttaagctggg gcgtgattta cttggtattg ggaagtgttc acttccgctc
     8101 atctaattat ctgcttctat ctcttttgtc gatagtcatc agtttttttt ctttgttttt
     8161 ttccattatg ttgatcagtt tccaagtaaa agaaagacaa catcaggaat cgcttcaact
     8221 gtatcaaata aatgaagaat attaccatca tctagaggaa ttcaaacatg attataaaag
     8281 tctactgttt tcattaaaaa ttactcttca ggaaaataaa aaagaaacgt tggaattttt
     8341 agaaatatta gaagatcatt ctgcagctat ttttgaaaaa ccgcaaatac aacagctaac
     8401 aaatattgta tccccagctg tcaggggtgt ctttttaaaa tttctagaaa aagcagaatc
     8461 ggagaaaatc ccgatccaag tagttattcc tgtcgaagtg aaaacaatcc cgattgatct
     8521 ggttgttttt attcgctgtt tatctatttt tatttctaac gcattcgatc atcgtgtagc
     8581 cgatcagagt ccgattacga tcaatctgct ggaagaaaaa gagggatcac gttttagtat
     8641 agcgaacaag gcaaatccaa cgaatttgac gctatcggag atggtcaaaa gaggaaaaac
     8701 ctctaaaaaa aatggcgggt taggtctata cagtgcgaaa aaaatgttgg atgtttacga
     8761 aaatgcagaa ctaaaaatcg aattttcaaa acagaatcac tatttttttg tagaaatgta
     8821 tctggcatct agaaagagca aatcagccta ctgacttact gtcaaaggat aagtttgagg
     8881 aaagtctaca cggagataga gacaacaaca cagcatattc aaaagaaaca gaagcgtgat
     8941 gaccgttgtc acccttctgt ttcttttgct atgtagttaa acaaagtagt tgacacttcc
     9001 gctacttagt attagtacga aagtatactt catgatacta agtagataag aagtgaaaag
     9061 cggaacccac gatcgtcaaa atgctaactg ctttgcttac accagataaa gggaagagca
     9121 atatcgctta ttttaggcgt ggagaagcaa gcaaatccct acgatcacaa aaaaaatgct
     9181 gacccagcag ttaagagagt tagaacaaga tgaaagtatc catcgaaaag cctataacca
     9241 agtcccgcct aaagtagagt actcgctcac cgaagaagga aaatcattaa gagagattct
     9301 agtagcgttg tctgtttggg aagaagaacg tatcgaaaaa caaaaagcct caagggaaga
     9361 agacacgcta ctgcatacac atggtggcta cctaaattat tgattttcta tgacatttat
     9421 tttaaataaa agaaaacagg agttttttta aaagcatcat ctatttttta tgtaaaaaaa
     9481 tggtatgatt aatcaagtaa gaagtgattg gaagaaggca agcaaatgaa tagtatggaa
     9541 aaagtcaacc agtttagaga tgaacgtaat tggcgacaat ttcataacga aaaagatttg
     9601 gctatctcta tttctttgga agcatctgaa ttgttagaac tttttcaatg gaaaacgccg
     9661 gaagaagtaa aagaaacaca attagaacga atcaaagaag aactggcaga tgtattgatc
     9721 tattcataca tgatggcaga caaccttcac ctggatctag atgagatcat tgaagaaaaa
     9781 ttgatcaaaa ataatttgaa atatcctgta gaaaaaagcg caggtgtacg aaaaaaatac
     9841 acagagttgt agaaatgtgt cattcggctg ttggaaagag caaaagaaaa acagtaaaat
     9901 gagtctggag ggagagaaaa tgaaaaaaaa aggaaaagca aaaagatggc tagtcaatat
     9961 cctcttgttc ctcttgttat tagtaggact agctttgatt ttcaatgaac agatcaagga
    10021 ttattttgtc agagagacag gagataaata tgctatagcg aatgtaacaa aagaagatct
    10081 gaaaaagaat aacgataaag atgtcagttt tgactttgat gcggtagaac caatgacgac
    10141 agaaggggtc atgaggtcgc aaatgagagg aacagatcta ccagtgattg ctagtatcgc
    10201 agtcccttca gtttcaatca atttaccggt tttcaaaggc ttggataata cgtcactact
    10261 ttacggagca ggaacattat ctccagatca agaaatggga aaaggaaatt atgccttagc
    10321 aagccatcgt gcaacgaacc ctgaattgct gtttacaccg ctagaaaatt tagagatggg
    10381 tgccaagatc tatttgacag atttagaaaa tgtgtataca tacaaaacat tttttaaaga
    10441 aaaagttgct cctacagata cccaattact gaatgaagtc gagggaaaag aaattgtcac
    10501 actaattact tgtggcgaca tggacgccgt cacgcgacta gttgtacaag gagaactgga
    10561 aagtgttaca tcgataaaag atgcgacaga tgatatgaga tctgctttca atctagagac
    10621 aaaaacattt taggatactg ggacataagt cgttacagat taaaaacagc caaaggatat
    10681 gaatcgataa ggaaaaacca atttgctgaa aaaaagagaa acgcaaagag gtgcatctct
    10741 gatttggttg ttttc
//
LOCUS       DAZUKC010000081        10696 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000081 DAZUKC010000000
VERSION     DAZUKC010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.083"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(303..788)
                     /locus_tag="ACG80T_002174"
     CDS             complement(303..788)
                     /locus_tag="ACG80T_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFS4841803.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    878..1223
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1268..1909
                     /locus_tag="ACG80T_002175"
     CDS             1268..1909
                     /locus_tag="ACG80T_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS4841804.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1928..2563
                     /locus_tag="ACG80T_002176"
     CDS             1928..2563
                     /locus_tag="ACG80T_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS4841805.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2581..3396
                     /locus_tag="ACG80T_002177"
     CDS             2581..3396
                     /locus_tag="ACG80T_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4841806.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3715..5151
                     /locus_tag="ACG80T_002178"
     CDS             3715..5151
                     /locus_tag="ACG80T_002178"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4841807.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5340..7787
                     /locus_tag="ACG80T_002179"
     CDS             5340..7787
                     /locus_tag="ACG80T_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFS4841808.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            8017..9084
                     /gene="rlmN"
                     /locus_tag="ACG80T_002180"
     CDS             8017..9084
                     /gene="rlmN"
                     /locus_tag="ACG80T_002180"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFS4841809.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            9074..9280
                     /locus_tag="ACG80T_002181"
     CDS             9074..9280
                     /locus_tag="ACG80T_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841810.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(9277..10380)
                     /locus_tag="ACG80T_002182"
     CDS             complement(9277..10380)
                     /locus_tag="ACG80T_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS4841811.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         3544 a         1814 c         2302 g         3036 t
ORIGIN      
        1 gatattctta caaaaatata aaaaagttta gttagaatat aatatttttt gttaaatatg
       61 actgaaatca aaagaataaa attccgattt ataatcgaaa aaagaagcat ttttatatat
      121 gcttcttttt tcgtagaatc aataaatatg gatttggcta actatggaag aatactcgta
      181 ttagttttca gcgttcatac tagttgttac attgtttgat tggccaaaag aatttctgat
      241 atattttgtg ctaaataaag aaaaagacgc ttgaattggg aaataagaaa actactctat
      301 cattacaaat tttcataaac tggcagatgt tctaagtaaa caagtatctt ttcaaagtcg
      361 gaaaaatcca taatcaacgt agtggtattg atatttggat gaaagcctac ggtatcttga
      421 cgatcaatag cagcatcgat cacgacttgg atttttttct ctttgtcgtg catcaatgct
      481 aatggagtca ctgtacctgc tggaacgcct aggaggtcag gaagctgttc ttcagaaaca
      541 aaagataagc gcttttcttt taattgttca gctagttgtt ttaaatcggc gtgcttttca
      601 tcaggcaaga tgacaagata gatttgtttt ccttttcttg atttcaataa tagatttttt
      661 acttgcggac caggtaggga aaaagggaca tttttgactg acgtaatcgc aggatgatcg
      721 actttttgaa aagagatacc cagttttttt agtagttcat aagcttcttg ttcggttgca
      781 cgactcatcg cgcatcaccc tccttttttt attagtgtat cataaaaaag agttgacaaa
      841 tgaaaaataa tcatgtaaat tacacgtaaa gttaaaacaa atgacatcga aaagaagagt
      901 agccgttagg tgtttttaga gagtctctgg ttggtggaaa gaggcagcag ttaaccgtga
      961 aacacatctt ggagtcgatt ttctgaagcg tatagtagga aaatccgggg agcccgttac
     1021 agctgcggtc gttgctagat gaccagataa ggctggcttt tccgtgagga gtcggtaaaa
     1081 aaaggtggaa ccacgtgatt aacgtccttt gatcttcgga tcaaaggact ttttgttttc
     1141 atctgaacgg caacgactgt gtgaggtttt ttctgcaagg aaaaaataaa cagaggtgga
     1201 accacgtata atacgtcctc ttggcatttg ccaagaggat ttttttattt taaaggagga
     1261 aaagaagatg gagtatttga ttgaagttat gccgcaatta ttagtaggag cactcacaac
     1321 attgaaatta tttttattta ccttgattgg ctcattacca ttagggattg ttttagcatt
     1381 aggattgtct ttgcatttta agccgctacg ctacttgctt caaacatatg tatgggttat
     1441 gcggggaaca ccgctgttgt tgcaactgat ttttattttt tatgggttgc cgacgattgg
     1501 gatcgttttt gatcgtttcg aagcagcact cattgccttt atcttaaact atgcagctta
     1561 ttttgcagag atctttcgtg gagggataca atcgattcct caaggtcaat atgaagcagc
     1621 gcaagttttg caattaagtc cctttcaaac ggtgacgaaa atcgtcatcc cacaagtgac
     1681 aaagattgtt ttaccttctg tagggaatga agttatcaac ttggtcaaag attcttcctt
     1741 gatctatata ttaggtttag gtgacgtaat gcgtgctggg aaaatcgcga tggaacgcga
     1801 cgcgacttta ctgcctttgc taggcgtggc aatcatctat cttacattga ctggtattct
     1861 aacaatcata atgaaacaag ttgaaggaaa attaaattat taccgctaac tgataggagg
     1921 aagaaatatg attgaattga agaaagtgaa caaagcattt ggtgaaaaaa aaataatcaa
     1981 agatttgaat ctaatcattc cagatgggaa aacattagct atcgtaggtc cttccggtgg
     2041 cggtaaaacc acgcttttac gtatcttggc tggcttagaa agttctgaca gcggagaatt
     2101 ttatttagac ggtgtaccgt tcaacccatt cgagcttaaa atgaaagaac aagttgtagg
     2161 agtcgtattc caagacttcc agctatttcc acatctgaat atttttgaaa acattacctt
     2221 agcaccaaaa atggtattga aacagacaaa agaagaatac accaaaaaag cagaagaatt
     2281 agcggcttca ttagggatta gtgatttatt gaatatctat ccttatcagc tttctggcgg
     2341 acaaaaacag cgggtagcga ttgccagagc attggctatg aatccacggg tattggctta
     2401 tgatgaacca acaagtgctt tagatcctga attacgccag caagtcgagc gtttgatcct
     2461 atcattgaaa gaagatgggg taacccaaat tattgtcacc catgatttag tatttgcaga
     2521 aaatgtcggt gatcaattac taaaagtttc cccaattcat taagaaagga gaagatagag
     2581 atgaaaaaaa ccagttttct attcgctttg atcgcaggtc ttcttctttt tgcaggctgt
     2641 tcgaacaaaa aaacttcagc agataattgg gcgactatca acgaagagaa aaaaatcgtt
     2701 ataggtttag acgatacatt tgttcctatg ggatttagag ataaagacgg gaagctcaca
     2761 ggatttgata ttgatttagc caaagcagtc tttgaacaat atgggattac ggtagacttt
     2821 cagcccattg actggtcaat gaaggaattt gaactgaaca atggaacgat cgatttgata
     2881 tggaacggct attccaaaac gccggcaaga gaaaagaaag tgcaatttac aaaaccttat
     2941 atggaaaacg atcaagtgct gatcacacct aaagaatcag gaatcacaaa ctttggacaa
     3001 atgaataata agagattagg tgcgcaaaat gggtcatctg gttatgatgt atttacgaaa
     3061 caaccagaag tattaaaaga gatagtcaaa aaccaagatg ctgttttgta tgatagtttt
     3121 aatgaagcat tgatcgatct gaaaagcaga cgggtcgatg gtctgctgat ggataaagta
     3181 tacgcagact attatttgaa acaaagaaac gaactagaag acttcaacat aacaaaaggt
     3241 tcttatgaga gtgaggattt tgcagttggt gcacgcaagt cagatagtac attagtgaaa
     3301 aaaatcaatg aagccctaga tcaactagag aaaacaggtg aattccaaaa aatatccgat
     3361 aaatggtttg gcgaagatgt tacgccgaga aattaacaaa taggggactg tgacaaaaga
     3421 cgtgtcacag tccctttttc cgaataaaca gtgttctcct gccaactcct tgtgactcgg
     3481 agctgaatgg caggctcaca acctcttggt aaacggtgtt caaaagccag tttttcggta
     3541 ttaagccaaa tttatgcaaa aatgtagaaa gccattttca caaaattttt cttactactc
     3601 gaaactaagc gactttttca caacctcctt tacataattt tattttgttg tggaaggggt
     3661 ttcgtgttat tctaaaagag gaacgattga aggttatagg aaaggaagag atgaatggaa
     3721 cacaaagaac ttacctcatt tccaaaagat tttctgtggg gctctgcttc tgcagcgtat
     3781 caagtagaag gagcttggca agaagatggg aaaggagaat cagtctggga tcgatttgta
     3841 cggatacctg gaaagacatt taaaggaacg aacggtgatt tggcagtaga ccactatcat
     3901 cgttataaag aagatattgc tttaatgaaa gaacaaggtc tgaaagctta ccgcttctct
     3961 gtagcttgga cacggatttt tcctaatgga cgaggagaag tcaatcaagc tgggctcgct
     4021 ttttatgaga gattgatcga cgaattgatt gaaaacgata ttgaacctat gttgacttta
     4081 tatcattggg atcttccaca agcattgcaa gatgaatata atggttggga atcacgtcag
     4141 atcattgaag atttcactca ttatgcagaa acattatttg aggcattccg cgggaaagtc
     4201 cattactgga ttagtttgaa tgaacaaaat atcttcactt cattaggtta tttgttagca
     4261 gcacatccgc caggtgtgac agacccgaaa cgaatgtatg aagttaacca tattgctaat
     4321 ttagcaaatg catctgtcat caataaattt cacgagttag aaatgcctgg aaaaatcgga
     4381 ccaagctttg cgtatacgcc aaattatcca attgacagta atcctgaaaa tgttctggct
     4441 gctgaaaatg cagaagacct gatggccaat tattggatgg atgtctatat gtggggaaaa
     4501 tatccgattg ctgccatgag gttcttagaa gaaaaaggat gggcaccaac gattgaagct
     4561 ggcgatgcag aattgctaga atctgctaaa ccagattttc ttgggatcaa ttactatcaa
     4621 acggcgacga atgcattcaa tccattaaac ggagttggtg ctggaaaaat gaatacgaca
     4681 ggaaaaaaag gttcatcaga agaaactgga gttcctggaa tgtataaaaa agttgagaat
     4741 ccatttgtcg aacgaacaaa ttgggactgg gagattgacc cagaaggatt aaggatcgga
     4801 ctgcgtcgta tcacaagccg ttaccgcatg ccggtactga ttacggaaaa tggcttagga
     4861 gagtatgaca agttgacaga agataaacaa atccatgacg actatcgaat caattactta
     4921 caaagccacg tcaaagccat caaagaagca atttcggatg gagcagaagt cctaggctat
     4981 tgcacctggt cttatacaga tttgcttagc tggctgaacg gttatcaaaa gcgatatgga
     5041 tttgtatatg ttgatcaaga cgaaacacaa gaaggatctt tggaacgata taagaaagac
     5101 agttattact ggtaccaaaa actgattgaa gaaaatggag agaaccttta gttcacatac
     5161 ttaaaacaag aaggtacggc tggatatggc cgtgcctttt cttgataaaa tcatttagaa
     5221 attaagaaaa actgtaaaat aagctgaaaa atgaaagaaa gcgtcagttc tttcaaacaa
     5281 aactttagtt cccttacttt tttttgtata atgaaacttg atgaaatgag ggataataaa
     5341 tggcacaaaa gaaacggcct actaaaaaaa ggaaaaagac gaaaaaaacg aaaaagcaaa
     5401 aacagcagca ggaacaaatg ctgacgattg gtgttgggtt tgccttgatt ttatttgctg
     5461 tcttcggctt tttaaaatta ggttttttag gaatattgat tgcaaacggc tttcggatca
     5521 ttgcaggaaa tacttaccaa atcctctgtc ttttattagc cgtattagga ttttggattg
     5581 tcattaagaa tacagagttt tcaattggta aaaaccgacg ctggtttggc ggcatactct
     5641 tttatttcgg tatcttatta ttgttacatg cccatttatt tggcaaactc catactgggg
     5701 aaccaaatat catggggacg acatgggatt tgttagccag tgatataaaa caaagtcaag
     5761 tagacaataa cgttggtggt gggatgattg gagcaatcct ttatcatttt acttacttct
     5821 tgattgcaca gcctggaagt tatttagcag cagtgctatt actggcagga ggagcattcc
     5881 tgttcagtaa tctggaaggc tatcagctgt taaatggtat ccaatcaatt ggagaacgag
     5941 tacaggaatt attggaaggt gatcctgcca agcaagctcg aaagcaagcg gcaaaggaag
     6001 agagaatgaa acagcgcgca gaagcgaaag aagcaaggcg cttagctgca aaagaagctg
     6061 cagaaaaaga agctgcagaa tatgagaaga aaaaagcggt gagccagcaa agggaaaata
     6121 ctgctgatga atggcaggaa ccagaaaata cagagccgga gcagctaagt tttgttccaa
     6181 tcgacagttt tcaagaaaat atccatcctg cgaatctaga gaaaccagta ccagatacac
     6241 caaaacagac caatacagca gaaggttttt cagatgagtt gccagaggat gacggtacct
     6301 cattagaatt tgagattgaa gcagaacagg aaaatcaaga ctatgaatta ccgacagttg
     6361 atttgcttga ttctatacca actgtggatc aaagtgatga gtataaaaaa attgaaaaaa
     6421 atatcggcgt attagaacaa actttccaaa gtttcggtgt cgatgccaag gtcgtcaaag
     6481 ccagcttagg accatccgtc acaaaattcg aagtccagcc ggctgtaggg gtaaaagtaa
     6541 gtaaaattgt gaacttaaca gatgatatcg cgcttgcttt agcggcgaaa gatgtccgga
     6601 tggaagcacc gattccaggt aaatcactga ttggtattga agtaccaaat ggaaagatca
     6661 gtatggtttc ctttagagaa atcatcgaag cacaaccaaa ccacccagat aaattactag
     6721 aagtaccttt aggacgtgat gtttctggac gggtacaaac ggctgatctt tcgaagatgc
     6781 ctcacttact cgtggctggt tcgactggta gtgggaagtc agtagcaatt aatgggatta
     6841 tcacaagtat attaatgcgt gctaagcccc atgaagtaaa actgatgatg atcgatccga
     6901 aaatggttga attgaatatg tacaacggca tacctcatct gttgacgccg gtcgtgacga
     6961 accctagaaa agcagcgcaa gccttacaaa aagtagtaca agaaatggaa gaacgatatg
     7021 aaaaattcgc ggcaaccgga gtaagaaata tttctggtta caatgaattt gttcaacaga
     7081 aaaacttgga aaacggaacg aaacatccaa ccttaccatt catcgtagtg atcgtagatg
     7141 aattagctga tttgatgatg gtagccagca atgaagttga agatgcgatt attcgtttag
     7201 cgcaaatggc tcgggcagcg gggattcata tgatcctagc tactcaacgt ccaagtgtgg
     7261 atgttatcac aggaatcatc aaagcaaatg taccttccag aatggcattt gcggtatcaa
     7321 gcggtacaga ttctcggacg atcattgatt ctaatggggc agaaaagctc ttagggcgag
     7381 gagatatgct attcttgcca atgggtgaga ataaaccgat ccgtgtgcaa ggagcattca
     7441 tttctgacca cgaagtagaa cgggtcgttc aatttgttac agatcagcaa gaagctcatt
     7501 atgaagagaa aatgatgccg acagatgaag ttgaaacgac aggtgctcca gaacagccac
     7561 aagatgagct gtttgaagaa gcaaaagcac ttgtcgttga aatgcaaacg gcaagtattt
     7621 cccttctcca aagaagattt cggatcggtt acaaccgagc agcacgttta gtagatgaac
     7681 tggaagctca tggagttgtt ggcccgtcag aaggaagcaa gcctcggaaa gtattcattg
     7741 agcaggaaga aacatcagaa acgttgccgg acgataatga catgtaaaac ttgtgtgatg
     7801 aagggttgaa aaacgaataa ccaataaaaa gacaaggcat gatgatacca aaagaaaaaa
     7861 tggttttttt ccatcttttc taatacggta tgatgtcttg tctttttagt ttttgtgaga
     7921 ttaaagttct attcgattta tttgtgaagt attgcattaa ggaaatgaat catgtatagt
     7981 atgtagcgaa actttgagaa aaagagggaa aatactttgg aaaaacagtc catttatggc
     8041 ttgacgaatg aagaattaat caattggttt atagaaaacg gcgagaaaaa attccgagct
     8101 gcccaagttt gggaatggtt gtatcaaaaa cgtgtaagta atttcacaga aatgacgaat
     8161 ctatcgaaac aattgatcga aaaattatcg gagcatttta tcatcaatcc tctaaaacag
     8221 atggtcgtcc aagaagcttc ggatggtaca gtaaaatatt tgtttgaact ccctgataaa
     8281 aacatgattg agactgtact gatgagacaa gaatatggct tgtcagtttg tgtgacgaca
     8341 caagtaggat gtaatatcgg ctgtacgttt tgtgcaagcg ggctgctcaa aaagaaccgt
     8401 gatctgactg ctggagaaat tgttgcacag atcatgatgg tccaacatta ttttgatgag
     8461 cgcaatcttg gcgaacgcgt ttcacatgtc gttgtaatgg ggatcggtga gccatttgat
     8521 aattacgaca atgtaatgga ctttttgcat atcatcaatg atgccaaagg attggcaatt
     8581 ggtgctcggc acattacagt ttccactagt ggattagcac acaaaatcaa ggaattcgct
     8641 aataacggct tgcaagtcaa tctggctatt tccttgcacg cgccgaataa tgaggtgcga
     8701 acatcgatca tgcggatcaa ccgaagtttc ccaattgaga aattgatgga agctgtggat
     8761 gagtatttag agaaaacaaa ccgtcgaatc acgtttgaat atatcatgtt gaatcaagta
     8821 aatgatagac cagagcatgc gcaacaatta gcagatctat taaaagataa gaaaaaatta
     8881 gcatatgtca acttgattcc atataatcca gtcagtgaac atgatcaata ttctcgcagt
     8941 ccaaaagctg atgtattgag attttatgat gtattgaaga aaaacggcgt aaactgtgtg
     9001 atacgtaaag aacatggtac agatattgat gcagcttgcg gacaattgcg aagcaagcaa
     9061 atgaaaaaaa cagttgccaa ataatcgtat cctactcgtt ttttcaatta ccataaaagg
     9121 agcagttttc ttaattaagg aactgttcaa cttaaataag agaaaagatt ctatagcgaa
     9181 agctataaag ctttttttga caagaaaaac cctcatcaag caaagaaatg tcctcaattt
     9241 gatgacagcc tgcttgatgg gggttttagc aattatttaa ctgattttct tatatccttg
     9301 ctggctttct tgtgtagcat gataagtttc tacgtcaaga atggcctgtt taaatttttc
     9361 tcgggtgatt tcataaggca catgtttaat atcatttgtt gccatggctt tatctaagat
     9421 tgcttctaaa tcggtcgtga cattgagatt catgtcggtt aacgatactg gaagttggtt
     9481 ctttttcata aacacataga ttttatcaaa caaatcgaac tgctgatcca tcatcaataa
     9541 ggtcagtaca ccataactga cagatgctcc gtgaaggtat ccctccattt tttctagaac
     9601 agtcgtaccg taatagaatg agtgtgccat agcagaatta tagttattgt taactaaaac
     9661 agaaaccaat ccggtagtaa caatgatatc cgaaacgact tgttcaattg catatgaggg
     9721 acgatcgtcg gcactatctt tcaatgcttg cgtaccataa gtcaagagac gatcaatgca
     9781 catttttccg atttggattc ctaatctatc cgtgtagcct agatcatcgc cacgagcaga
     9841 aaaagtagat tcgacttctt tggataaagc atcgccaata cctgcccata agtatttatc
     9901 aggtgcttcg gcaataattg ctgtatcaat gaaggtgtgt acaggtggtt ttgaaggaaa
     9961 gaagagtccg tccattacgc cttcttcgtt atagatgaca cataccgcag tgacgggcga
    10021 acagttcgaa ccaatagtgg gaaaggtgaa gactggttta gctagttttt caccaatgac
    10081 ttttacagta tcgcaagcac ggccaccacc gactgcaaac aggacgtctg ctttttggac
    10141 actttcatgc tggatcaact gttcaatatt tccataagtt gaattacccc cgtaccagag
    10201 actggcgaca acctctattt cctgtgtcaa agctactttt attttgtttt ctgcctttga
    10261 aagtgcagtc ttccctccaa tcagcactac ttttgtccca tattctttga cgattccatt
    10321 tgctttttca tacgcatctg ttccaatggt ataatttggt aagaaaacac tttgactcat
    10381 attcatttcc ttctttcttc gttgaaacgg gttaaaattt taaagagtga tcgatctgac
    10441 tgcggctgta gatggagtag aggtgaacct aagacaggat aagtgatgaa taaggtggca
    10501 tgaacacctt gaaatcatca ataagtatcc taaaaacgaa caagtacaaa aacattgaaa
    10561 acgacaataa tgaatttttg ttcctcttag tatttctacc gattcccttt gcttttttgt
    10621 aattatctaa aaattctaaa aattaagatt attatatgaa aacaggtttt atttgtcaat
    10681 tcataattgg tttttt
//
LOCUS       DAZUKC010000082        10649 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000082 DAZUKC010000000
VERSION     DAZUKC010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.084"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..656
                     /locus_tag="ACG80T_002183"
     CDS             33..656
                     /locus_tag="ACG80T_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841812.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /gene="trpX"
                     /locus_tag="ACG80T_002184"
     CDS             1106..2155
                     /gene="trpX"
                     /locus_tag="ACG80T_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4841813.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="ACG80T_002185"
     CDS             2152..3033
                     /locus_tag="ACG80T_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4841814.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="ACG80T_002186"
     CDS             3036..3806
                     /locus_tag="ACG80T_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4841815.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="ACG80T_002187"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="ACG80T_002187"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFS4841816.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="ACG80T_002188"
     CDS             5117..6280
                     /locus_tag="ACG80T_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS4841817.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="ACG80T_002189"
     CDS             6397..6807
                     /locus_tag="ACG80T_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4841818.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="ACG80T_002190"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="ACG80T_002190"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFS4841819.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9380..9664
                     /locus_tag="ACG80T_002191"
     CDS             9380..9664
                     /locus_tag="ACG80T_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841820.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9824..10060
                     /locus_tag="ACG80T_002192"
     CDS             9824..10060
                     /locus_tag="ACG80T_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS4841821.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10057..10455
                     /locus_tag="ACG80T_002193"
     CDS             10057..10455
                     /locus_tag="ACG80T_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFS4841822.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3722 a         1773 c         2253 g         2901 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     9421 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     9481 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     9541 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     9601 ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg
     9661 ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt
     9721 tcgtaagaaa aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa
     9781 tgtacttact atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa
     9841 gtataaaatt agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga
     9901 attcatgggt gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt
     9961 acgaatgatc aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa
    10021 tcaatatgaa gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa
    10081 attatcaaaa tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta
    10141 aaagatgcaa atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa
    10201 gaactctatc ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa
    10261 catccttttc ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa
    10321 ttgaataatt gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt
    10381 accactagtt cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa
    10441 accgcaataa aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa
    10501 ggaatatgct gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga
    10561 aaatatggat taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg
    10621 aaatagtggt ttagaactag tgggctgat
//
LOCUS       DAZUKC010000083        10641 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000083 DAZUKC010000000
VERSION     DAZUKC010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.085"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..525
                     /locus_tag="ACG80T_002194"
     CDS             <1..525
                     /locus_tag="ACG80T_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841823.1"
                     /translation="LEKAIPYIAAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTI
                     SNEINQYREREPEIQPAEVKQEHKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRT
                     LHLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKN
                     EIYYEQQLSQKERV"
     gene            586..2055
                     /locus_tag="ACG80T_002195"
     CDS             586..2055
                     /locus_tag="ACG80T_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFS4841824.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVGDDRLDHAVAFLWKDPQTGETVGASYQGTIVDFNRFGKRGTYKHIDKNPTPNHGFN
                     LKIGDPKHLKFFESSIDLLSYAALNREKLQDAWLVSMDGLKHHVISHYVEESISELRR
                     KQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDPFTNKKIRCERGIPNDWQVPKEYKAT
                     YEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVSAHDVQSTFGKMLAKGEPVETIDLKE
                     ACTTVAKELKVCERADGTYNFDRFYSRKANIKDVNAGILLSYKAEQYYKGYKKHEHEF
                     VPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            2066..4175
                     /gene="topB"
                     /locus_tag="ACG80T_002196"
                     /pseudo
     CDS             2066..4175
                     /gene="topB"
                     /locus_tag="ACG80T_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
     gene            4191..4340
                     /locus_tag="ACG80T_002197"
     CDS             4191..4340
                     /locus_tag="ACG80T_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841825.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            4421..5017
                     /locus_tag="ACG80T_002198"
     CDS             4421..5017
                     /locus_tag="ACG80T_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFS4841826.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            5030..5929
                     /locus_tag="ACG80T_002199"
     CDS             5030..5929
                     /locus_tag="ACG80T_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFS4841827.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKEQKLAYQRHQQMNREMER"
     gene            5932..6416
                     /gene="ssb"
                     /locus_tag="ACG80T_002200"
                     /pseudo
     CDS             5932..6416
                     /gene="ssb"
                     /locus_tag="ACG80T_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            6438..6695
                     /locus_tag="ACG80T_002201"
     CDS             6438..6695
                     /locus_tag="ACG80T_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841828.1"
                     /translation="MAKKISLTMDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVF
                     VYLVCSYLLKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(6854..6988)
                     /locus_tag="ACG80T_002202"
     CDS             complement(6854..6988)
                     /locus_tag="ACG80T_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HFS4841829.1"
                     /translation="MLKSIKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK
                     "
     gene            7131..7391
                     /locus_tag="ACG80T_002203"
     CDS             7131..7391
                     /locus_tag="ACG80T_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841830.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            7840..8046
                     /locus_tag="ACG80T_002204"
     CDS             7840..8046
                     /locus_tag="ACG80T_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFS4841831.1"
                     /translation="MRKKFDYLGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIEEKGKKR"
     gene            8046..8297
                     /locus_tag="ACG80T_002205"
     CDS             8046..8297
                     /locus_tag="ACG80T_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841832.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGCELHYQENLETYENEYWLTKLEN"
     gene            8313..8750
                     /locus_tag="ACG80T_002206"
     CDS             8313..8750
                     /locus_tag="ACG80T_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841833.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCFEIGVKPSTIKPSEYQALE
                     LTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            complement(8861..9088)
                     /locus_tag="ACG80T_002207"
     CDS             complement(8861..9088)
                     /locus_tag="ACG80T_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841834.1"
                     /translation="MKQNTSITKNTARFNCTAALSRISISPTNSTNNKGIIFFKWITF
                     FLLSYRALPVYFAPPKRRGRLVRNKNHSLSY"
     gene            9186..9374
                     /locus_tag="ACG80T_002208"
     CDS             9186..9374
                     /locus_tag="ACG80T_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841835.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IACIGQYLYCIRGRKLYA"
     gene            9492..9698
                     /locus_tag="ACG80T_002209"
     CDS             9492..9698
                     /locus_tag="ACG80T_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841836.1"
                     /translation="MKYNLLDHKRMWVEKLEEDLEQLELLGHNKTSTVYKEGLKRTKK
                     AQKELATLESRVQKKEVDLTHTYL"
     gene            9708..9941
                     /locus_tag="ACG80T_002210"
     CDS             9708..9941
                     /locus_tag="ACG80T_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HFS4841837.1"
                     /translation="MADKRKEKIQKLLALAADVNDEESVSALAKAQQLMMDYDLTEDE
                     IFNYHEQQRIEAVETHVIYRGRPQKWLYRLFRN"
     gene            10263..10466
                     /locus_tag="ACG80T_002211"
     CDS             10263..10466
                     /locus_tag="ACG80T_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucocin A/sakacin P family class II
                     bacteriocin"
                     /protein_id="HFS4841838.1"
                     /translation="MILGIVLLSVSTLGITVDAATYYGNGVYCNTQKCWVDWSRARSE
                     IVDRGVKAYVNGFTKVLGGVGGR"
     gene            10456..>10641
                     /locus_tag="ACG80T_002212"
     CDS             10456..>10641
                     /locus_tag="ACG80T_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841839.1"
                     /translation="MEDKKEKLFDLIANAYNSKSYNNVEVKEGFLQDAKKIREDKPYP
                     EIIISVYKHLSKLKAEER"
BASE COUNT         3880 a         1739 c         2233 g         2789 t
ORIGIN      
        1 ttggagaaag ctattcctta tattgcagca tggacgaaga atggtcagac gttggatgat
       61 aaggaagctg cggaacgtgg gaaaatcatg aatgatgtca gccgtgtcgc aaatgaattc
      121 attcaaacca tttctaatga aatcaatcaa tacagagaaa gagaaccaga gatccaacca
      181 gcagaagtga agcaggagca taaaaatact gtagaaacgg cagaacaaaa gaatattgtt
      241 cctgttggtt cactttggat cgacaagaaa gacgggaaag taatggaaga agatacgggt
      301 cgaacgttac atttgaagga tgcggcgttt attgaagatc cacagcataa tcaagtaatt
      361 ttaaaaggaa aaagttatgg aaaagagatc caaacggcgt taccttcaga agaatttgcg
      421 ctaggaaatt cattccgatt ttcacttgag acagtgtcag aagtggcaaa acgaaaaaat
      481 gaaatctatt atgaacaaca attgtcacaa aaagaacgtg tataaaacaa gtaaaaaaga
      541 ctatctgaaa aacggatagt cttttttctt ttgggaggtt ttaaaatgac cgagaaaaaa
      601 atgagtctta ttgatcgatg taaacagatc gatatagtgg attttgctcg aaacaatgga
      661 atggctgtag tcaataaagg acgagactat cgcttagaag accatgattc atttgttttt
      721 gatcgaagaa aacaacgttt ttattggaac agtcaaaata ttagtggcga cattattgaa
      781 ttagctaaac tttttttcat agacaaagag atccaagacc ctaagcaaca atttaaagca
      841 gccactgatt ttattctaaa gaatgaagac aaaacagaac gagtggaaaa tcttcatttt
      901 gagacagaaa agtacaaaga tcaccccgtt gattatcaac cactcacaga aaaaggaagg
      961 aattatctga aggaagaacg aaaactacca gagtggctga ttgattatgc agaaaaagaa
     1021 gggttgattg ctgaactaaa gccgaaacat gaacggcaaa attttttagt tggagacgac
     1081 cgtttagatc atgcggttgc ttttttatgg aaagatccac agacaggaga gacagtaggg
     1141 gcaagttatc aaggaacgat cgtcgatttt aatcgtttcg gtaaacgggg aacgtataaa
     1201 cacattgaca agaatcctac gccaaatcac ggatttaatt taaaaatagg cgatccgaag
     1261 catttaaaat tctttgaaag tagtattgat ctgttaagtt acgctgcatt gaatcgggag
     1321 aaactacaag atgcctggtt agtttcgatg gacgggttaa aacatcatgt aatcagtcat
     1381 tacgttgaag agtcgatttc tgaattaaga cgaaaacaaa catttcctca atcgattgaa
     1441 atatgtgtgg acaatgatcg tgcaggacat attttttatg aaaaagaaca gatgaaggga
     1501 atcgttgatc cttttaccaa taagaaaatc cgttgtgagc gaggaattcc aaatgattgg
     1561 caagtaccta aagaatacaa agctacttat gaagcagtcg caaaagaaat gagtgttgaa
     1621 cctgaagcaa ttatggccat acacaaaact gaaactaatt tacagctgac aaatcaattg
     1681 gtgtctgctc atgatgttca atctacgttt ggaaagatgt tggcgaaggg cgaaccagta
     1741 gaaacaattg atttgaaaga agcatgtacc acggtcgcga aggaactgaa agtatgtgaa
     1801 cgcgcagatg gaacatacaa ctttgatcgt ttttatagta gaaaagccaa cataaaagac
     1861 gttaatgcag gaatccttct ttcctataaa gcagaacaat actacaaagg ctataaaaag
     1921 catgaacatg aatttgtacc tgaagtgaaa aaggattgga atgatcaatt gaagcatgag
     1981 atccagcaac aagaaatcag aaagcaaaaa cgtgcgatgt tgtttcaaca aggcagacaa
     2041 caagaaaggg agtgaagtcg tcactatgaa aaccgtcatt ttagcagaaa aaccgtcaca
     2101 agcgaaggct tatgccgata gcttttctaa agctacccga aaagatgggt attttgagat
     2161 ccaagatcga ttgtttacag gagaaactgt gatcacgtat ggttttggtc atttagtcga
     2221 gttagattcg cccgatatgt acgatgaaaa atggaaacaa tgggcattag aacatttgcc
     2281 tattttcccc aatcaatacc actatcatgt tcctaaagat aagaaaaagc agtttagtgt
     2341 cgtcaaacga cagctgcagt cagctgatac gattgtgatc gcgactgata gcgatcgtga
     2401 aggggagctg attgcctggt caatcattca acaagcaggc gccaatcaag gaaaaacctt
     2461 taaaagactt tggatcaact ccttagaaaa agaagccatt tatcagggat tccaacagtt
     2521 acgagacgca gaagagacct atccaaaatt tgaagaagta caagcacgtc agatcgccga
     2581 ttggttaatt gggatgaacg gtagcccact gtatagttta ctcttgcagc aaaaaggcat
     2641 accaggaagt ttttctttag gtagagtcca aaccccaaca ctctatatga tttatcaatt
     2701 gcaggaaaag attagaaact tccaaaaaga accatatttt gaaggaaaag cccaagtaat
     2761 cgctcaaaat ggcgcatttg atgcgaagct tgatccaaat gaaacccaag cgacacaaga
     2821 agcatttgaa gcctatttaa aagaaaaagg tgttcaatta ggaaaacagc caggtacgat
     2881 ccttcaagtc gaaacagaga aaaaaagtgc ggctagccca cgtctttttt ctttatcgag
     2941 ccttcagtct aaaatgaatc aactcatgaa agctagtgcc aaagatactt tggaagccat
     3001 gcaagggttg tatgaaggaa aatatctaag ctatccgcga acggataccc cttatattac
     3061 ggaaggggaa tatgcgtatc tgttggatca cttagacgaa tacaagcatt ttttaaaagc
     3121 cgaagcgatt ccgactccta ttcatacacc gaatagtcgg tatgtgaata ataagaaagt
     3181 ccaggaacat tacgcgatca ttcctacaaa aacggttatg acagcagcgg cttttgaaca
     3241 actaagtcca ctgcagcagg ctatttatga acaagtgtta aaaacaacgg tggccatgtt
     3301 tgcggaaaag tatacctatg aagaaacaac gatcttgacg caagttcagc agcttcagtt
     3361 aaaggcaatc gaaaaggtgc cgctagatct aggttggaag aagttatttg gaaaagagag
     3421 cgacggaaaa gagaaggaag aagagccgct acttcctaaa gtgacaaaag gagaaatggt
     3481 gacggtggat ctacaagttc ttgaaaaaga aaccaagcca ccacaacctt ataccgaagg
     3541 tacgttgatc actgcaatga aaacagctgg gaaaaccgtg gatagtgaag aagcgcaatc
     3601 catattgaaa gaagtggaag gaatcggtac agaagcgaca cgggcgaata ttattgaaac
     3661 cttgaaacaa aaggaatata tcaaggtaga gaaaaataag ctcgtggtga cgaataaagg
     3721 gattttgctt tgccaagccg tggaaaaaga gccgctgctt acgagcgccg aaatgaccgc
     3781 caaatgggaa agctacttgt taaagatcgg tgaacgaaaa ggaacacaaa ccacttttct
     3841 agccaatatc caaaaatttg tctctcactt attggaagtc gtaccagggc aaatccaatc
     3901 cactgatttt ggatcgacgt tacaagaagt gaaagcagcc agtgaaaagc aagaagccgc
     3961 gcgtcattta ggaatttgtc caaaatgcca ggaacaagaa gtcttactct atcaaaaggt
     4021 ggcagcttgt acgtctgaag cctgtgattt taaaaggtca gaagggttca tttgaagcaa
     4081 cgattgtttt aaaagaagac ttcactacag gctttgaatt ttctgaaaag aaaaaaacga
     4141 acttcaagaa aagaacacgc cgcactgcaa aataatggag ggttaatctt atgcgtaata
     4201 ccacacacaa acaacaagaa atcgaaaatc aaaaagccaa aattcgttca gaacaggaaa
     4261 aaatgcgtgc cagcaaacga agactgcagc aattgaagaa ccagcagagg attcaaaaag
     4321 aaatcgaatt agatcgataa aatcttccta actattgtat acgtataaac ttatgttata
     4381 ctgtttttat aagtttatac gtatacaaag gagggagaaa atgacaaaag caatggtata
     4441 caagctgcta gaagaattta atggaacttt ggctttgaag gatgccaaaa aagcgggcat
     4501 ttctcctgta acgatcaaac gtatggtaga tcgaggggag ctggatcgag agtatccagg
     4561 ttttttcacc ttacctggtc aatttcctga tgaattgttt atggcgcaaa aaaaatatga
     4621 acgcggcatt atttcacata ttaccgcgtt agatttgtat gatttaacag atatgatccc
     4681 tagacagatc gatctgactg taccttacgg ttatcacgtt tctgaaaaag gattgaagga
     4741 attcgcagtt gagctacact atacaaaagc ggaatggtat gaattaggta agattgaaat
     4801 aaaaagtcgc tatgggaatc ctattatagc ttatgatcca gaaagaactt tatgtgacat
     4861 ttggaatccc tggtataccg ttgaggatga aatcaaagta aaagcaatca aaaactatat
     4921 ggagtcggac agaaaaaatc ttagaaaatt gaatgagtat cgacgaattt taccaacaga
     4981 caaaacgatg cgttcttata ttatggcttt aaattaaagg agaagataca tgacacctac
     5041 gcagttaaaa gcaaaagtga agaatatttc tagagaaaaa gaagttgatc cgcagcttgt
     5101 tttaagacat tttatgatgg aaaaattctt ggaaaaaatc tcagagtctc cttatcgtga
     5161 aaattttgtg ttaaaaggcg gcttcttgat tggctcaaag tatggcattg aaaatcgcac
     5221 aaccaaagat attgatacga ctttacggga aatgaaagta accaaagaaa cattaacaac
     5281 ggttctaaac gaaatttttt caacgccgac gaaagaaggc attcaatttg agattcaagg
     5341 aatgaaagaa acgagagaag ccgattacta tccaggattt agcttacgtg tgctggcaca
     5401 tttggaaaac atgcggccag attttaaggt agatgtgact acaggtgact cgatctatcc
     5461 tgcaacgatc actcacagtc ataaattgat gtttgaggat cgaacgattc ctttggaatc
     5521 ctatcctaca gaacaaatta ttgctgaaaa attaagtgca acctttgatt ttttaacaga
     5581 taattcgaga ggaaaagatt tttatgatct ctatactatt cctaaaatgg agaagatcga
     5641 tgagaagacc ttgaaagata gtgtgagaaa tacatttaca cgtcgaggaa aaactgatcc
     5701 gttaaagata tactatgaaa aggatatgga tgtactgaaa aatagtcacg agctaaaaga
     5761 tacctggaag aaatatcaaa aaagcaatcc ttatgctgca tcgatcacgt acgatgagac
     5821 aatggattcg attagtaatt tgatggaaaa agtgattcac gtcgaagaaa aagagcaaaa
     5881 actagcttat caaagacacc aacagatgaa tagagaaatg gagagataaa gatgattaat
     5941 aatgtgacat tagtaggacg cttaaccaaa gatcctgatt tacgttatac cgcaagtggt
     6001 actgcagtag cgacttttac cctagccgtc aaccgaaatt tcacgaatca aaacggcaat
     6061 cgagaagccg attttattaa ctgcgtgatt tggcgcaaac cagcagaaac aatggcgaca
     6121 ttagctaaaa aaggtagctt gattggtgta gttgggcgga ttcaaacgcg gacttacgac
     6181 aaccagcaag gacaacgtgt atatgtgact gaagttgtag cagataactt tcaactatta
     6241 gaaaataaag cagcgacaga gagccgtgcg cacgctgatc aatcgtccac cagtccaagc
     6301 accgccacct ttgaacaacg gatacagcga cgcctaacaa caatggattg aacgcttcac
     6361 agaatccatt tggtggacaa tcaatcgata ttagcgatga tgatctaccg ttttagtttg
     6421 agaaagaagg agtaagcatg gcaaaaaaaa ttagtctgac gatggatcta ttgatacccg
     6481 taattgcatt tatagacggg atttctttgg gcgtcactga aaatcctttg gtaaatagaa
     6541 ttgcgaaagg atcactattt cttgtattcg tgtatcttgt ctgttcttat cttttaaaaa
     6601 gagcaggaaa ggataattcc ttttggcgat tgactaaagt cacagtagca gcgacagctg
     6661 ttatacttac ggctattcta ccgctttata tgtagagtta acgggaaatc gttgaacttc
     6721 ttgcgaaata ttgctataat gaacatgaaa aagaggagtt gcgctaacaa cccctcatta
     6781 acagcaccga ttaaagaacg gtggcagaat attagttatt tggaaataac cgctgacctg
     6841 ctaaagtaga cggttatttc tttttgtcat ttttaagcat atttacaatc aaactaatca
     6901 aagcaatggt aaacatacca aaaccaagaa tggtttgaat tgtctcatat gcggacaaaa
     6961 ggcttactcc ttttatagat tttagcactt acattcataa gcaccaccac ctttccagaa
     7021 gtcgccaccg tcttcaaact ttactgtctt gactattata gcatatctaa aatcgaataa
     7081 aaattctcaa ttttaactgt ttttgctata attttagcga ggtgatcgtg atgaacaaat
     7141 atgttggttt gcttgataaa atccgagtaa taaaaacaca accattattg gttcgcttta
     7201 ctttacaaac aattcatgaa tcgattaatt gtgtagttgc agatattgaa attatcgata
     7261 aattgttaat tatggatgat ggaaaataca atattgccgt cacaggtcat ttcaataaac
     7321 ggaatcaatt agtcattgaa tcgatgcaaa ttagaaaccc tgatcatttt acgcgatcaa
     7381 tgggcatata aataaaagaa gagctgcaat cctaatgtag gactgcagct ctttttgttt
     7441 tacatagaaa caggaagaaa gggaaaggcc tgtttaggaa ggcacaagtg ccttctcaat
     7501 tgaaaaacct ttctctcact ccaaccgcca ccgccgttcg agaagaaagg aagtgaacgt
     7561 tgcacgttca ctaagataag gggtcttgct ttgtaagaca aaataggaaa agcttcctct
     7621 caccaaaagg ttcgttcctg ttcgctatct ctttattgat ttgccagacc ggcaacaagt
     7681 tgccttttat caccaaaaag cgacaaggac aagggtaaat ggcacgctgc gcgcccttgt
     7741 ccttgtcgct tttcgttgat tcattctggc caacaaatca ataaagaaag aactaacgaa
     7801 aggaagtaaa acctttccta agaggaggca gaatcaatca tgcgaaagaa atttgattat
     7861 ttgggtgttc ccttcagcga attattccca aactatcatg caccacatac tgtcgaatgc
     7921 gattgtggag aaagagcgaa atgtatcaaa agctattgtt tgtatcaatg tccaacatgc
     7981 gggaaaaaat ataccttgtc ttatggcgat tatattttga ttgaggagaa aggaaagaaa
     8041 cgctgatgaa aacacaacac aaattattga ctgtaaaaga agtcgttcaa attttggtgg
     8101 acaaagaagt catttctgaa aaagtagcag aagctttact tattccgcgg tactggacga
     8161 tagaaacccc tttagtagag gatgcaccga ctatgctatt tgaagaccta gtgaagattg
     8221 caggatgtga actgcattat caagaaaact tggaaactta tgaaaacgaa tattggttaa
     8281 ccaaattaga gaactaaaaa ggagaaaaaa atatggctca aaattactat gacgaatttg
     8341 tcaaattacc tttggataaa atggcacaaa aaatggagga tatgacgttc ctatacaacg
     8401 aaacacgtgt tccaaaaaaa cattataaag aaaaattgtc ggtagctgtg gaagaaatga
     8461 ttgaatcagg tgtcgaaatg aatcttattg cgacgtacta tcgaacactg gaagaactga
     8521 aaaagcaaaa tgcaaaatgg tttttccaag ccttactatg ctttgaaata ggtgtcaaac
     8581 caagcactat taaaccttct gaataccagg ctttagaact aacgtatgcg aaatttatcg
     8641 aaacgaaaaa agccaagact gtttcaagtg aatggttaga ttattttgaa aatatcaata
     8701 agtatggcgc atattacacc atgaaaaagg aagacaacga aaatgaataa ggtgatttat
     8761 caactaacaa cggattccat atcaacgatt tgaaagaaaa aaaggtcaat aatcatttat
     8821 accgaaaagt gagaatgtgt ggaataagga caaaaaaagc ctagtaacta agggaatggt
     8881 tcttatttcg caccaaccgc ccccgccgct taggtggtgc gaaataaacg ggcaaagccc
     8941 gataacttaa gagaaagaag gtgatccatt tgaaaaagat tattccctta ttgttagtac
     9001 tgtttgttgg cgaaatactt atgcgagata gtgcagctgt acagttaaac ctcgctgtat
     9061 ttttggtaat actggtgttt tgtttgatct tttggcggtt tgtattagtc ctcgtgagtc
     9121 tactattcaa gatgattgca gccatatttt tcatggggtt atttcgatga aaggagagag
     9181 taccaatgcc aattgcaata ggaaataaac ggttacccgt gacattagat gaaaagagac
     9241 aaaaagaatt gcagcaacta aagcagaagt acggcaaaag tgaatccagg attatgtgta
     9301 ttgcgttaga tttattgatt gcctgtattg gacaatatct ctattgtatt cgaggaagaa
     9361 agctttacgc ttgaatgcga taagttaacc cgatatatcg atccaaaatt ctatgaggat
     9421 aaaaacctgg catgggaact ggctttatag acagagtgct agtgcaagta aaggaagaaa
     9481 ggaagatcac catgaaatac aatttattag accataagcg aatgtgggta gagaaattag
     9541 aggaagactt agaacagttg gaattgttag gtcataataa gacttcaacg gtgtataaag
     9601 aaggactaaa acgaacgaaa aaagcacaaa aagaactagc aacactggaa agtagggttc
     9661 agaaaaaaga agtagactta acacacactt acctttagga gtagaaaatg gcagacaaga
     9721 gaaaagaaaa gattcaaaaa ttactggcgc ttgcggcgga tgtaaatgat gaggaatcag
     9781 tgagcgcatt agccaaagcc cagcaattga tgatggacta tgatcttact gaagatgaga
     9841 ttttcaatta tcatgagcag caacggatag aagcagtaga aacgcacgtg atctatcgtg
     9901 gacgtccgca aaaatggctg tatcgtttat ttagaaatta agataaaaaa ggctagttaa
     9961 caggtttgtt gactggcctt ttttgatatg tttgtacatg ttttattgtg ttttaatttt
    10021 tctctttttt atagcaagct ccttttataa taaatattcc gaaaagtgaa gaccattttt
    10081 tatttattaa gactattatt attagtgaga taaaatttga ttgatcttac agaatattgg
    10141 tgacgattat gacactaaag atactattta tgaaaatttt gcaaaaatgt tctgaaaatt
    10201 acatataatt gttttataaa aaagggaggc aattatatga agaaaaaatt tgttagtatt
    10261 ttttgatttt aggaattgtt ttattgagtg tatctacttt aggaattaca gtagatgctg
    10321 caacttatta tggaaatggt gtttattgta atactcaaaa atgttgggta gattggagta
    10381 gagctcgctc tgagattgtt gatagaggag taaaagctta cgttaatggt tttacaaaag
    10441 tgctaggtgg agtcggtgga agataaaaaa gaaaaacttt ttgacttaat tgcgaatgca
    10501 tataattcaa agtcatataa taatgtagaa gtaaaagaag gatttcttca agatgctaag
    10561 aaaatacgag aggataaacc atatcctgaa attattatat cagtgtacaa acatttatct
    10621 aaattaaaag ctgaggaacg c
//
LOCUS       DAZUKC010000084        10605 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000084 DAZUKC010000000
VERSION     DAZUKC010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.086"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..2224
                     /locus_tag="ACG80T_002213"
     CDS             278..2224
                     /locus_tag="ACG80T_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4841840.1"
                     /translation="MIERHVNLLKLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLED
                     YVVNYQVTIERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFG
                     DKPVSLTHLSQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAI
                     KQLVFYYANDMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLIST
                     LVIQCNRLLKGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLC
                     RQLFAHRVTNNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLR
                     LKKGIKIQNPLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIE
                     DQSKANNIIIICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISS
                     LSFDAGGIPLVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKF
                     VQSSLVKTGVQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALP
                     HANPSMVIESNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQK
                     EMVDRLLQVHDSEKLLRIFQQKGE"
     gene            2227..2682
                     /locus_tag="ACG80T_002214"
     CDS             2227..2682
                     /locus_tag="ACG80T_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4841841.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            2696..4024
                     /locus_tag="ACG80T_002215"
     CDS             2696..4024
                     /locus_tag="ACG80T_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFS4841842.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            4058..4342
                     /locus_tag="ACG80T_002216"
     CDS             4058..4342
                     /locus_tag="ACG80T_002216"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4841843.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            4344..4859
                     /locus_tag="ACG80T_002217"
     CDS             4344..4859
                     /locus_tag="ACG80T_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HFS4841844.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            4875..5537
                     /gene="rpe"
                     /locus_tag="ACG80T_002218"
     CDS             4875..5537
                     /gene="rpe"
                     /locus_tag="ACG80T_002218"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFS4841845.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            5544..6116
                     /gene="hxlB"
                     /locus_tag="ACG80T_002219"
     CDS             5544..6116
                     /gene="hxlB"
                     /locus_tag="ACG80T_002219"
                     /EC_number="5.3.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0043800 - hexulose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HFS4841846.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(6303..7823)
                     /locus_tag="ACG80T_002220"
     CDS             complement(6303..7823)
                     /locus_tag="ACG80T_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFS4841847.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(8066..8251)
                     /gene="tnpB"
                     /locus_tag="ACG80T_002221"
     CDS             complement(8066..8251)
                     /gene="tnpB"
                     /locus_tag="ACG80T_002221"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFS4841848.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            complement(8235..8417)
                     /locus_tag="ACG80T_002222"
     CDS             complement(8235..8417)
                     /locus_tag="ACG80T_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841849.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            8658..8876
                     /locus_tag="ACG80T_002223"
                     /pseudo
     CDS             8658..8876
                     /locus_tag="ACG80T_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            9371..9970
                     /locus_tag="ACG80T_002224"
     CDS             9371..9970
                     /locus_tag="ACG80T_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4841850.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            10034..>10605
                     /locus_tag="ACG80T_002225"
     CDS             10034..>10605
                     /locus_tag="ACG80T_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbiH family protein"
                     /protein_id="HFS4841851.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIG"
BASE COUNT         3501 a         1670 c         1984 g         3450 t
ORIGIN      
        1 aaatacgaat tttagtgacg ccattatatt gtttttattt ttcaattgta atatttcata
       61 ttacttccat aataaggagt attttctatt ggaatatctt agctctttta aggatttctc
      121 cttttttgtt tctcattttt taagattatt tggatttcct ttacaacaaa cttccacaag
      181 tattattgac atagatgtaa ttgagggaag ggggttccag tgctagaata aataatgaaa
      241 agaggtaaca aatgctagcg ttaagaggtg aacaagtttg attgaaagac atgtaaattt
      301 attaaaatta ttaagtcgac aagaagactt taaacccgct tcttttttta gtgataaatt
      361 ggcagtgtcg accaagacca tttatcaaga tattgaattg ttggaagatt atgtagttaa
      421 ttaccaagta acaattgagc gattaccttc tttgggtatc aaactcatag gtgataggaa
      481 aaacgttgaa catttgattt catcggtaga gaattatctt gaaaaagata tttattctcc
      541 agaaaaaaga cgtgtcgaga tcattaaaga agttttgttt ggtgataagc ctgtttcttt
      601 aactcatttg tctcaaagtt ttatggttag taaaacatcg ttatacaatg acctgagaat
      661 tatcaataaa attattggca gtggagaagt taagttgcag tcatctgcag aaggaattag
      721 ttttactggt aatgaacaag caattcaacg agcaatcaag caacttgtat tctattatgc
      781 gaatgacatg gaatcaaaaa cattttttca agttttaaat atgttgtttg attcaaaagt
      841 tattagtgtc attcatgatt tgctattcaa tgattacagt gaactgactg aaaaggtttc
      901 tgattactat gttcggtcat tgatttctac tttagttatc caatgtaacc gtttactgaa
      961 aggtcatcat ttaactgttg aagaagaatt cctcttcaat agcattcgct atatggaaac
     1021 gtacattgtg gcaaatggtt taattgaaga attgaaggca aaattgaatc ttccatttta
     1081 tgacgcagat aaagaatatc tatgtcgtca actatttgca catcgtgtga caaataattt
     1141 aaaaaccagc aatgaagagt acgctatgag cgttcataaa ttgattgaaa gaatgagtga
     1201 gattgaaaaa attgatttga ctcaaaatga tcgattatat caatccttat tgtatcatgt
     1261 gccagcaatg gtccttcgtt taaaaaaagg tattaaaatt caaaatccgt tattagaaaa
     1321 tatcaaatct caatattcag aactctttag catagtatgg tacgccatgt ccatccttga
     1381 agaagaattt gatgtgactt taacggacga tgaagtttcc ttaattttga ttcattttca
     1441 gattgctatt gaagatcagt ccaaagcaaa taatatcatc attatttgcc aatacggtat
     1501 ttcttctgcg caattaattt acaataaagt tcgacggttt ttaccagcaa aagataatgt
     1561 agaaatatca actttagata agtttcattt agctgaaatt ggggaagttg atttgattat
     1621 ttcctctctt tcctttgatg caggtggtat acccttagta agggtatctc cattggtaag
     1681 taaaacagat tatatgaata ttatggaagc ttatactaaa tatttaatag aagaaccttc
     1741 gcgatttatt caaaatgaac tgattcctaa agcggttaaa gttcccacca tatctaaatt
     1801 tgttcaatca tctttagtaa aaacaggagt acaggtggaa agcaaagaag aatgtttgga
     1861 tttgatgatt tccgaactag aagccaattc attagttact gaagagtttc gtgcttctat
     1921 cttctctaga gagaaaatgg ggaatactaa tttagaaagt ggtgttgctt taccgcatgc
     1981 gaatccatca atggtcattg aatccaacat ttttattgta gcattaaaaa atgcgattaa
     2041 ttggggcgat cgaaaagtca aattagtcat tatggttagt ttagctgaag aagatgtatc
     2101 tgagattaga ggagtatatg aagaacttta ccaatttatt gggcaaaaag aaatggtcga
     2161 tcgcttgtta caagttcatg atagcgaaaa attactaaga atttttcaac aaaaaggaga
     2221 ataaaaatgt atctagataa agaattatgt ttatttcaat tagaagtgaa agatcaaaaa
     2281 gaattgtttc aagtaatgtc tgaacaatta aaaaacgctg gttgcgtgaa agatagtttc
     2341 ttagaaggta tcaccaaccg tgaacaagaa tttccaactg gattagaagt aaatcaaatt
     2401 gggtttgcta tcccacatac agattccgca catgtaaata gctctcaaat ctgttttgcc
     2461 tcattaaagg aaccactggt attttcagat atgacagata aatctcatga aatacctgta
     2521 cgtttagtct ttatgttggc aatgtcacaa cctcatgaac aaattgatac actacaaaac
     2581 ttagttagcc tatttcaaaa tgaagaaaaa gtaaatgaat tacttgcatg taccacgaaa
     2641 gaagcattta ttgagttagt acattctgca ggagtttatt aggaggaata gagagatgga
     2701 tattgtatta aatgcctttg aatggtttat tggattaggg tcaaacgtct ttttaccact
     2761 gattattttt gtaatcggga tattatttgg attaaaacct ggtaaagcat ttgtttccgg
     2821 cgttactgtt ggtatcggtt ctattggttt aggattagtt ttaagtttat tatcagatgg
     2881 tttaggaaca gcaattcaac aaatggggga acaatacggt ggtggcctga atatcctaga
     2941 tattggcgta ggtgttggtg gacctttggc ttactctaca tcattgggaa ttttaatgat
     3001 tccaatttca ttaattctta acttcatatt tgtggttttg ggttggacaa aaacattgaa
     3061 tgttgatatt tggaactttt ggttcgcaat tttcctaggt atgattactc aatctgttac
     3121 gggaaacttt tggtttggct taattggcgc gattgtcgca gtaattttac aatggttctt
     3181 agcagatcgt acacaaaaaa ctgttagcga atttttcggc tatccaggaa tctctatcac
     3241 acatatgatg ggattatcag gagtactatt tgccaaacca gtaaattggg tttttgatcg
     3301 tattcctgga tttaataaaa tagatgcaga tgcagaaaca ttaacaaaac gtttcggtgt
     3361 tttaggtgac acagttgtta ttggcctaat tatcggtatt gtagtgggta ttatggcagg
     3421 ctatgacgta gcaggaattg gaaacttagg aattatgact gcggcagtga tgaagattat
     3481 gcctaaaatg gtagcaatgt ttatggaagg tttaatgcca attgcagaag cggcgaaaga
     3541 atttacgaat aaaaagttaa atggtcgcca agtaaacatt ggaatggatg cagcgttaac
     3601 tgtgggtcat ccaactatta tgtcaacttc tctattgctt gtaccaatct cattattgct
     3661 agcactaatt cttccaggaa ataaagtatt accttttgga gatttagcat tctttgcttt
     3721 cgccatctgt ttaatgattc cttattttaa aggaaatatt gtgagatcat tagtaggatg
     3781 ttcgttgtat gttgttacaa cattatatat ggcaacatgg ttggcaccaa ttatcactga
     3841 tgtgtttaca ttagcaagtt tcgatgtggg gacagcagga ttagtaacta tgttgttatg
     3901 tggattatgg ccagtagggt tgtttgtttt attagcatct tactttggtg taattggaat
     3961 aggtggatta ggagttgttt tagtaggtgc ttctatctac atgaacaaag tgaagaaagc
     4021 ttaatcataa aattaaaata agaaagaaga gatcaatatg aaaaaattat tagttatttg
     4081 tggagcaggt catgctactt ctacaattgc agtagcaaaa ttatcaaaat ggttggaaga
     4141 agaaggatta acagatcaag tgaaaattta tcaatctaaa attgctgatg agctaaataa
     4201 aattgatgaa tatgatgctg tagtaagtac aacaattgtc cctgatgtaa ttaaagctaa
     4261 agtaacgaat ggcttgggct tattaacagg tattggtaca gatgccattt atgcagatgt
     4321 aaaagaaaga ttaggtgttt aatatgcgtt ttcatcatga cactttgact attgaaacag
     4381 tttcagggcg tccttcgtat catgatattc gaaaagaact tttagataca gttgatacag
     4441 caggtattga gaatggaatg ttagtaattt cgagcccgca tacaacttgc tcactctttt
     4501 ttgaagaaac gatgcatgac acaaactttt ttggtgatga ttatttacat gtagacatca
     4561 acaatgtaat ggaaacgatt attccaaaaa tgacgagtga aaatcaatac aacagtccgg
     4621 ggccaaaaca cattgatttt ggaacaagat tatctgatcc caattaccct gcagaaaaat
     4681 gggtcatgct aaataccgat gcacatttac gttcatctat ttatggaacc aactcaatga
     4741 ctttaattgt tcaagaaggt aaacttttat taggagaaat gggccgtgtc tattttgtcg
     4801 attgggatca actaagagag cgaactagaa caatcaatct catgattatg ggagaataaa
     4861 cgggggaata gagaatgaca attgctccat cattgttaaa tgcacctatc gcagaagtta
     4921 aagagttatt tgaaatctta aaagaaaaga atgttgatta tttgcatatt gatgtaatgg
     4981 atggctgttt tgtaccagat atggcttttg gaccaagtgt tattaaagat attcgtccac
     5041 taacagacgt tgtgtttgat gttcatttga tgattgatac accagaaaaa attgtcacaa
     5101 gatttgtaga agcaggagca gatattgtta ccttccacat tgaagctacc aaacacgcaa
     5161 tgaaggtcat tcagttagtc aaggaagcag gagcaaaagt agggattact tttaatcctg
     5221 caacaccagt cagttttatt gaaccagtat tatctttagt cgatcaagta ttagtaatga
     5281 caatcaatcc aggaacagag aataagcatt ttattcaaga gacagtagtt aaaatcgaac
     5341 agttagatgt aatacgcaaa caaaatgatt atacgtatga tattgaagta gatggcaaga
     5401 ttgataatca aacgattaaa gtttgttcta aagcaggggc ggatattttt gtatcaggcg
     5461 gtttcatctt caatgggaaa aataccccgc aagaacaaat tgataaacta aatcaatcat
     5521 tagaggaagt gaagtaaaag atcatgacca ataaagacca aaataaaaac tatttagaag
     5581 cgattatctt agaattacaa aataactcaa aacgtattaa ttctgtagag ttagaacaat
     5641 ttagtgagaa aattgttcaa gctaaacaca tctttctagc tggtgcagga cgctctggaa
     5701 ttgccatgca agcatttacg aatcgcttaa tgcatttagg tttttcagtc agccttgtcg
     5761 gtgaaattag ttcaccacat tcgcaacctg gagatttatt aattatctgt tctggctcag
     5821 gtgaaacagg tagtttaaaa aatttagcgg ccaaagcaaa tcaaagtggt gtaagcattg
     5881 ctttagttac catgaaaaaa gactcgtcga ttggtcaatt agcagattct gttcttgtat
     5941 taccaggtat taccaaagat gaaaatcaac gagaagaaga tactttttca caacctatgg
     6001 gttcaagttt tgaacaatta gcatttctca cctttgatag tttagtgtta tctttaatgg
     6061 ataaaacagg tgaaagctca gaaacgatgt ttgctcgaca tgcagatttt gagtagtaga
     6121 ttttaaagat aaaaaaccat tctctaaatg ctaattattc agcaaatatg agaatggttt
     6181 ttttatttga ttagacataa cttaataatt cagtagtttt gagcgtaagc gtgtcataga
     6241 atggtaccta aacacaaata gcccgatttg atacactcaa atcaggttat ttgtggctta
     6301 tattatttac agatttcttg tatctgctga ttccatggca aataagcgcg tagcgcttca
     6361 ggtgtcatcg aagacagatt gggaatctct gtaaaaagat gatttaaata tttgcggatg
     6421 tttaacccat tcgcaacagc tgtctggata atactcaata taattccatt tgatctagcg
     6481 ccttttaagc ttttagaaaa tagccagttt tttcgaccga tcactaactc tttgatggca
     6541 cgctctgctc ggttatttga aagttccaag cgtccatcaa gcaacacaat ctgaagtccg
     6601 tctttatgat tgactgcgta ttgaaccgcc tttccaagct gcgattttgg caaataagca
     6661 aaactctcac accaatgaaa aaattcagtc atcacaggct gcaacgtttc cttcctcgct
     6721 acaaaccttt cttctgcgct aagcttatta aaggtccgtt cgattcgaaa catcttgtca
     6781 cagaagtcta atcctatttt ggcatgactt tttggggacg gttgtttacc caatgcttca
     6841 aaaaactttc tgcgaacatg cgcccaacaa taaacattgt ttaccggcag ctttccatag
     6901 gccgcgtaac catcagtatg caagaaaccg ctaaatgatt ctaaaaaact ttttggctca
     6961 tcaacggaac gcgaatttgc gtgctcgtag agcacaactt gtttttcttc gcttttacct
     7021 gaagcatata accagtaaaa ggtcttttct ttatgagtat tcaatacccg atagcttgtc
     7081 tcatccgcgt gtaagacctc ctgattgctt aattcttgat gaaggcaatt gtagattggc
     7141 tctaaaacca tttcacttac cttgatatgc caattagtga tttttttacg atcaagaggg
     7201 aaaccaagtg catgccattc tttttcttgt cgatatgccg gcaccttttt aacaaatttt
     7261 tcccaaatac tctgtgtcac aattgaagag gaacctagac tattattgat caacggttta
     7321 ggaacaggcg cttttttgat ttttgtttct cctaatgctt cacattctct gcaatgatag
     7381 gcatgttggt agatgacttg tttttctata tgggcaggaa catacaatgg ctcttctcta
     7441 agccttgtcg caccgatatc atgtaaagta tgaccacagt cttcacacaa acaatcggca
     7501 ccttttaatt cataatgaat aggaacttca ggcagacctt ctaatgaaag tgctttgagt
     7561 cccttgcgtt ttctgcgttt aaaagaagat accgtaatgg gttcctggct ttcttgtcca
     7621 gtgtgctctg gttcattaaa agttgagtcg tctaataaat cgatttgccc atctacgggt
     7681 ggtgtttctt tttttcgtcc aaacaagcgt cgattcattt cagcaatttg ttctctaaga
     7741 agagtatttt cctgttgctg gatttcaagc aactgtgtta attgcttgat ttgtacaaga
     7801 agttcagatt ccatttttgt catttttaca ccaccattct agcgattttc ttacatctat
     7861 tataccattt tatgacaacg aaaaacagac aaaatccttg atttaacaag gttttcgtct
     7921 aatatatcgc accttttttc gtttctttta gcgtacattt ttcttcgatt gaaaggccag
     7981 tcagtaagac ttcttttctg gaacgcggcc attgaaggta cccactttca aatcttttgt
     8041 acattaatac aaatccattt tgatcctagt aaagagcctt gaagcgatct ttcttcgtac
     8101 cgcaaaataa gaaaagcgct tgatcgtaaa catcaaggtc atattcttct ataacgaaag
     8161 ctgctagtcc atcaattcct tttcgaagat ctgtttttcc acaaacgata aaaatatgtt
     8221 tcaatgcagt tgcatcaatg agcattggca ttcaactctt ttaacaaggt atacaaaaga
     8281 tatttatcca ttccattata aataaatatt tccttatctt ccgatttgat tctgcaagct
     8341 agttttggct tggtgtcaat ttgcgatttt tgagccgcac ttgaaaattg aactggtaca
     8401 atatcttttt tattcattcg tttttctcct tctatatttg atagtttaat tatagaagaa
     8461 agataatcgg cgtacgagat actatcctat gacacgctta catttaattt aatattttgc
     8521 ctcaaattag ccacactttt tatagaacta agaggaaaaa tgaagttttc ttcctattaa
     8581 tatagttgtt tgaaatctta aatatagcgt tttacgatgt aatgggaagt atggtgaata
     8641 aatcaactat gttcgtacga agcatttact caactaattt aatcgaatct ttcaataaac
     8701 aaatcaagaa atacagccac cgcaaggaac agttccaaaa tgaagagtcg atggaacgtt
     8761 tcttagtctc gtcttttgat acttacaacc aaaaattcct aggtcgcagt cataaaggtt
     8821 tccaacaggc cgaaggcgag ctcgaacaaa tgctgagtca accgatggag aattagaaac
     8881 aatctacagt agggaagaac cattgacaca aaattattga cgctcccaac taaaagtgtc
     8941 aaaataatga tgaaatcatt tagttcttat aaattagaaa agagaaaata aatattccat
     9001 atctacttta ttggatccgt aacatgacta aattctgaat actttttaaa gcttatcatt
     9061 tggtaaaact gaatataagt taattatata gactgaaaat tttgaatttt cagaatagaa
     9121 taacttaaaa gtactgacaa aattttttaa aggggcaata ttaatgttct gagataatca
     9181 aatcctaccg aaaatatcaa aaatccaatg tgacacaatc ggattgtgtc tcattcgagg
     9241 gaatcacaca atcccacgtg gctctaggca gaattgaatt tttgcttggg gctttttcct
     9301 tatattgatt ttgaatgaga cttattatat gttataatgc aattaagtat cattggagag
     9361 ggcgaagaaa atgaagacgg tagaacctat tcgagacaaa aaaaagatcg atgcgatgaa
     9421 ggcaatcttg gcttcgggaa aatatggcca acgaaatttg gttctttttt cgattgggat
     9481 caacaccgcc tatcgcattt ctgatttgcg tcaattgaag ctggccgacg tgctggaaat
     9541 ttcacgcggt cgcgtggtgg tcaaagatcg attagcgatg aaggaacaaa aaacagctaa
     9601 acataattcg gtctatattt ccaacaaatt gcgaaaaacg attttggagt acgttcaaca
     9661 ggaatttcct gatcaactcc ttcagcaaga ttttgacggt tatttgttcc ctagtcgaaa
     9721 agggacgaat gaaccgctca accgccaaag tctgtggcga atcattcatg atgctggcac
     9781 ggcaatcgga ttgaaagaca ttggcccaca ttccatgcga aaaacgttcg gttactttct
     9841 ctataaacag gggaccaaaa cagaaatcat tcagtccttg ttgaaccatt cttctcagcg
     9901 cgaaacattg cggtatattg gtattacaca agaagacaaa gatacagctg taaaaagctt
     9961 agatttataa tataaaagtt aagtttaaac atggatagtt ttaaattagt agattgagtg
    10021 ttgggaggaa agaatgagta cattatatat tataggaaac ggttttgatt taagtcatgg
    10081 tttaaaaact agatacactg atttttcaaa atgggttgaa aaaagtcata gtgaaatatt
    10141 cgataaaata gacacaatga taaatgaaaa taatgaggtg agaagtacag ctgatgataa
    10201 gataggttgg tttaattttg aaagtagttt gcatgagtta attcaaagtc cagtctttga
    10261 agattatgta gaagagtcta aggattatct cataggttat ggacatgaag attggtcaga
    10321 ttctgcccat catgattttc aaaatatggt ttatgattat gttgtttctc tagaagaatt
    10381 acaacaactt ttcaaggaat ggatacgcaa agaaatacag ccgacaatag aacataaaat
    10441 tccaaaatac caattttcgt ccgatgtact attctttaca ttcaattata cagatgtttt
    10501 ggaaattcta tacaagcaac cgaaagaaaa catatatcat attcatggca gttatgacga
    10561 tataataatc ggccactccg agaattttga caactttata ggaga
//
LOCUS       DAZUKC010000085        10269 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000085 DAZUKC010000000
VERSION     DAZUKC010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.087"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(559..1599)
                     /locus_tag="ACG80T_002226"
     CDS             complement(559..1599)
                     /locus_tag="ACG80T_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFS4841852.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1948..2274)
                     /locus_tag="ACG80T_002227"
     CDS             complement(1948..2274)
                     /locus_tag="ACG80T_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841853.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2261..3049)
                     /locus_tag="ACG80T_002228"
     CDS             complement(2261..3049)
                     /locus_tag="ACG80T_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4841854.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3417..3707)
                     /locus_tag="ACG80T_002229"
     CDS             complement(3417..3707)
                     /locus_tag="ACG80T_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFS4841855.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(3843..4022)
                     /locus_tag="ACG80T_002230"
     CDS             complement(3843..4022)
                     /locus_tag="ACG80T_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841856.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4019..4369)
                     /locus_tag="ACG80T_002231"
     CDS             complement(4019..4369)
                     /locus_tag="ACG80T_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841857.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4362..5687)
                     /locus_tag="ACG80T_002232"
     CDS             complement(4362..5687)
                     /locus_tag="ACG80T_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFS4841858.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            6137..6751
                     /locus_tag="ACG80T_002233"
     CDS             6137..6751
                     /locus_tag="ACG80T_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4841859.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(6916..7920)
                     /locus_tag="ACG80T_002234"
     CDS             complement(6916..7920)
                     /locus_tag="ACG80T_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841860.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFYSKEKLADFSNIAEMIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLE
                     LKTLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITA
                     CEIGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEK
                     RNKLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNK
                     SQITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD
                     "
     gene            complement(8379..8636)
                     /locus_tag="ACG80T_002235"
     CDS             complement(8379..8636)
                     /locus_tag="ACG80T_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFS4841861.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(8629..8898)
                     /locus_tag="ACG80T_002236"
     CDS             complement(8629..8898)
                     /locus_tag="ACG80T_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS4841862.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(9029..9208)
                     /locus_tag="ACG80T_002237"
     CDS             complement(9029..9208)
                     /locus_tag="ACG80T_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841863.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            9306..9542
                     /locus_tag="ACG80T_002238"
     CDS             9306..9542
                     /locus_tag="ACG80T_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841864.1"
                     /translation="MKQNTSIAKNTTKFKCTAAAWRISISPTNRTNNRGMILFIWISF
                     FLLGYRVLPAYFAPPKRRGRLERLVRNKNHSLSY"
     gene            complement(9589..>10269)
                     /locus_tag="ACG80T_002239"
     CDS             complement(9589..>10269)
                     /locus_tag="ACG80T_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841865.1"
                     /translation="TEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFIQRVEEQLYL
                     ESETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETEKYIMNVYLERSELVENFVSY
                     TICRKHYYSESEYYSIFESMELMEEEMEELNSKDATIDDYYLVFTRFLTCGTESIVLM
                     GMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNELDRKRMPYQ
                     RFERKKRQ"
BASE COUNT         3132 a         1876 c         1472 g         3789 t
ORIGIN      
        1 cgttgcgggt aataccgtaa cactgaaggg cacgcattaa ggcgtcatct taatgctttc
       61 ctgtaggaca ctgacggaga ttaccagtct cccgagcctt acaggggcct ttcttttttt
      121 tgtattttca attatcacaa tttatcgtcc aaaagtgcag aacgaaaatc aattcatcac
      181 aagcatatca gacaattaaa tttaaaacaa ctaaaaatat acaaaaaagt gtaattgaat
      241 attagcagca ctgcctgaaa agaagtaata tcaggctttt cagcttgggg aaataagtga
      301 attacctctc ttcatgaatt attataactc aaaaaagcac atatgcaaac actagttccc
      361 tatttttttg aatgaaaaat aactaaaata tctaacgatt atgactttaa aacctacgtt
      421 tgtgattgtt ttcataaact tttaatctaa aaactccgga ataatagcta tttatttgta
      481 acatttgtaa taaaattaaa atcataaaaa agttctaatt taaaattgat catttttata
      541 gtgatcgctt tttatcaact actctagcca attttccgta ctaactgttg gtaattcgtt
      601 aaatgtttct ctttgttcgt ttgcaagctt tgccgcaata gctttttcga caaaccaatt
      661 ttttacataa acgaaaatta gattctgcaa attgtttact ttttcagttt tttgtttttg
      721 ataagctttc agcaaagtca tatataatcc tttgtcagca tcaattccat aagattccag
      781 ttcttcataa acaacaactg tttgctctaa ttcttcagct tttttctttg cattatgaat
      841 agtttggacg gttttttgag cgtcttcgta agtactagaa aatgttttta tcaagtttaa
      901 tacattttct ggtacaaatg tatttatact gtcctctttc atgatgtcga cgaagttatc
      961 tagtaaaact tgattttgta actgatcagt ttcagtgtct attatgtgtc tattagtgtc
     1021 tatagaatta gaatagaata gattgtctgc gattttcgca attacggttg agtccttgat
     1081 atgactggat tttgccgtat ttgcgatttt cgcaattacg gtggtatcat tgatttctcg
     1141 cggttttgcc gtatttgcga ttttcgcaat tacggtggta tcagtattgt cttcctcttc
     1201 catgatttta aagacgtctt gattcgttac ttctggcttg cctaagtaca ataaattagg
     1261 catggattca atcttaccgt cacgtttatt tactcttcct ctcttctgtt tcagaagacc
     1321 tgctttttct aattcctttt tgattttaga tactttacct tcgcggcatt gtaacaattt
     1381 cattaattct tcaactgtga atatgaaaaa tatattatca tcagaatcta cccagttatt
     1441 tttgactgaa taatcaaatc gatctttaag tagcatataa gcaatttttg catcatttga
     1501 tagatccatg tatttttcgt tagtaaaaaa taccttaggc atttgataat atttttcacg
     1561 ataaatttct tttgatttgt aataattaaa atcattcata gccaatcctc tttctgtagg
     1621 atagcgtttt gttcaaaact atgttacaat gaattaaaca aatatatcta tccactatat
     1681 atttttctac tgctgataag tcgccaaact ttgttaaagc agtagtcttt ttttatcttt
     1741 ttttagttta ccttaaaaaa taaattaaag caaaccgctc tatttcaagg tttaagctca
     1801 tagtaactat atcacgatat tacaataata ttatatcacg atatcgtaat attacaatat
     1861 gaagataagc gacttatcag ctttgatact actgattttt ataaaaaaaa tatcacgata
     1921 ttatgatatc gtgatattga atgtaaatta aattttattt tgtttctttg ctagtttata
     1981 tccttcaatc aagtattctt caggtatgga ctttaatagc gcatcttcta taacgtctgg
     2041 tttacttttg tctgattcag caatagtcgt ccaatatttg agtgctgaca ttagaattgg
     2101 atttatacga atgctatctg ctgttcttac actacttcct tgatctttta tgactttact
     2161 tatatttttc ttggttaatt cgatttcttc tacttctttt gatttaaaat tctttttatt
     2221 tttatttcct agtaatctac ttactgcagc ttgactgcga ttatcttctg ccatcaaaat
     2281 caccaacttc ttttaaatat tcttctgtaa atgctttata gtcatcttca gctgttgagg
     2341 gtttattaaa attgattaga tctgatccag cagcttgcgc ttcgtcaacg gttattgtcc
     2401 tacgaatata agttttgaaa atttttgcac caatgactct agaaatttcg gttgctgcag
     2461 acgtcatttc tttagaaatg ttataacgct tattatactt agtaaataga actcctcgta
     2521 cttcaagctt gtcgttcgta tattctttta ccgtttcaat agtttcactt aactttatta
     2581 gtccttttaa tgagaaatac gcagcttcaa ctggaatgat aatttcatca gaaacagtaa
     2641 atgcgttgac tgtaagcatt cctaaacttg gtggacaatc aattataata taatcatagt
     2701 tgtccttgat tggttgaagt actcttttaa gcctgtgttc acttcctact tgagtaaatt
     2761 ccctctctgc agaagaaagg gctatgtcgg ctggcaaaac atcaattcct ttatagtcca
     2821 gaatagcatc tgtaatgtca gcctcccctt ttaatacttc ataaattgta ggttggtttg
     2881 ttccatctat atcgaaacct acattatctg tagcatttcc ctgaggatca atgtccacta
     2941 aaagtacatt tttaccgtaa acaaatttca aacatgctgc taaattaacg ctagtcgtag
     3001 ttttagaaac tccacctttt tgattggcta ctgacaatat tcttgccatt aaaatatccc
     3061 ccattcatct tatattataa tataattata tcataatatt gtgatattgc aatatcgtat
     3121 atgcattata ttgtaatatt gtgtgattat tttgttttaa agatatatat cggttcggat
     3181 ataaagatta ttcatatgtt attcgaaatt aaaataatca ctttttatta ctttaaaatt
     3241 ttttattaat gataagaaat atgttttgcg agcagcaact agaataattt aaacattact
     3301 aaactgtcta tattgttact aataattccc ttcttatagt gcattttctt agaaaaaaac
     3361 aatattacga tatcatgata ttaagatatc gtaatattat acaattgtga ttgatattat
     3421 atatgttttt ttattttttt atcatgttta atcttgaagg aatcatattc tttttctaaa
     3481 gacaggagct tttcataaac atcagacatg tttaaactag aggccaggct taaatcataa
     3541 acgaatgaag caggtaatga ttttattcta cgttgtcggg ttatccagct tgcaaccgtt
     3601 ccttgtgggt aggcatataa ctcacaaaat tgttctacag tgattcctaa attcttgata
     3661 atgtaaacat tgatcgggtg cgagtaaaca aatgtattga tagacatggt gcgtaagcct
     3721 actttctttt attattgcgt ttgcaataat agtttaacac acgcattatt ctagtatcta
     3781 ctcctatttg taaaaacaaa aaaggattca tagagataac tctatagaat ccgtaaaata
     3841 ccttatatat ataacgggaa gatataaaac ctaaaattat gataacctgc tgcagcttct
     3901 acatagaaag agaagcgctc aacaccaacc tcatttatca aacgttggcc ataaccccaa
     3961 atctcttctg atagctgatc aaaggttata tactttagct catattgctg gataatgttc
     4021 attctaatcg actccattta ttatcctggt aaaattcgat atgacgtatt tcgtcgtagt
     4081 gaattttgtc ttttccaacg tagatcccca gctcatcact acctaagatc ttgccaatga
     4141 tatcttctgg atagtatcct tcagcgtttc gttcttcttt ttgaatagat actgatctat
     4201 tttttatgac tgcagcgttc aatacttccg caatctcttc agtcgtcatt tgttcttttg
     4261 ctaaattttg actggttcgt tgttcattgt cggcatcaat ttcttcagta tgatcagata
     4321 aataaaaccc tgcccacttt aaacgtccac gatcattgta atcaatcatt tttaattccc
     4381 ccatttccgc ccgcatgacc accaacaagt gtgcttctag taatgcttct agcgccttct
     4441 aacattgaag aagcatgtac aatcgacgta aaaccatatt tttgccgaat cttgtcaata
     4501 attttatcta aattttcttc gtcaatttgc ttttgtggat cactaaataa atccagctga
     4561 agcgaatcgg tgtacacaag cttgctatag gttattccaa catgccttac ttcttgtcct
     4621 tcataatact tatcaaacaa gaaaagacaa taattctgca attcctttgt attatctgtg
     4681 atagggatct tcatctggtg tgaaaaccct ggtctcgttt ctaagatcga cgtgccaatg
     4741 tgtaaatgga cacaacctgt tttacagtta tgctgcctaa tgcgtatcgc tacttgttcc
     4801 gccatttctt ttaccaccag ctcgatttca tttcttctag tataatctcg tggtaacact
     4861 tgtgaatttc catatgattt ttcctttgtc ggttctggtg ggattgctat atctgttcga
     4921 tcgatcccat tagcatggaa gtacagctgt aagccaatca cacctagtct attcttaatc
     4981 gtataaggat tccagttagc aagatctcta atgctcataa tccccatctg attgagtctt
     5041 tttttcattc gagagccaat tccccaaaag tccgtcattt caggaatatt ccatactttt
     5101 tcaggaacgt tttcgtacgt ccattccgca acaaatcctt cattatgctt tgcttcgttg
     5161 tctaaggcta gtttagccaa aaggggatta tctccgacac ctactgtagc aatcaatcct
     5221 agctcttctt taatattctc ctggatcatt tgagccagct tctttctccg ttgacttcgt
     5281 gttccttcag tcgtaaaaag attcagtgat cgggtcactt ttaagattga ttcatcgatc
     5341 gagtaaatca gtagatcttc atcagccaca tatcttctga aaatattatt tacctgcata
     5401 tttcgcttga tatataggtt catacgtggt ggaacaacgt gtagtgtttt aggaaatggt
     5461 tgtggtaagt cacgtggtct actcacattt gtaataccat accgcttttt tgcttcagga
     5521 gaagaagcta atatcaatcc tgaaccagta ttatcagctc gactcataac aacaagttct
     5581 gttgttaatg gatctaaatt tctttctata cattcgacac ttgcgtaaaa gcttttcata
     5641 tcgattaaaa agtaatcatt tactggctct tttgaataat ccatcataaa taacactcca
     5701 ttctttcaca ttatacaaac atatgttcga tattgtaaag aagaaatagc taaatacata
     5761 aaaaaagaag cataggtatg ctatcaccct gcttcttctt ttgatttgta tgatcaattt
     5821 attctatcac tttactaatt aatttgaaaa tctaataaaa actctcactc taaaagagtg
     5881 agagactgca aattagagta cggacttttt tggagttccg ttctttaata ttaacaaatg
     5941 aataaaatcc ttacaacatt tgagaataag ttatcagtag acacttacaa aaaattctca
     6001 tactatttcc tccatcatta gtttttgtct atataaggtt taccctaaat agacactggt
     6061 ataacattga aaaatatggc ttcctcgtgt atgattaggg tgtattttaa atagttgcag
     6121 aaagtaggta aaagttttga gagttgcata catacgtgtc tcatcgatcg atcaaaatga
     6181 acaacgccag attgaagaaa tgaaaaaatt cggtgcagag agaattttca tcgagaaaca
     6241 atctggcgca acgattactc atcgacctgt tttccaagaa gcattagatt acgtccgaga
     6301 tcaagatatt tttattgtgg aagctattga tcgacttgga agaaattatg acgagatcat
     6361 cgcttcagtc aattacttga agaagaaaaa tgtgaagctg atcattacta gtttaccgat
     6421 catggctgaa gcaattggca atcctctcct ggataaattc attaaggatc tgatcatcca
     6481 aattttggcc atgatcgctg aacaagaacg gactgaatcg aaacgtcgcc aggcacaagg
     6541 aattaagatc gcaaaagcaa acggcgtcta caaagggcgg cccaaattat acagtgcaga
     6601 cgcaaaagat ccccaacgtc gccttgttta caaaagcatc gttgaagatc ttaaaaatgg
     6661 tgttgcgatt gctaagattg ctaaagacta caatgttacg agacaaaccg tgtatagaat
     6721 aaaaaaagac agcatggtca atgacaaata gttgatcatg ctgcttttat tatcttctca
     6781 tctttaattt cctacatata attactatct ataaaattaa gtcttactca gaacagataa
     6841 agcataaaat tccaacaaac cattttttac ttaactaaat aaagagtctg ttaatataat
     6901 cttcacttat ttacgttaat cccctactgt atttgttggt tccttatcta tattttctac
     6961 aaaatcctta ttatcttgtg atttagcttc gtataattct ttgtatgtcg acacattggt
     7021 tatactaatt cctatagcta gttcttgaga ctctactttt ctctctagct cctttgacaa
     7081 agtgatttga gatttatttg gttccgttgg agtcaattct atagattctt cataaaaatc
     7141 tctatacata aagctatcat taattttata aaaaaaatta aatgttttta gcactgccat
     7201 ataacattca aaacattctt ctatagttgg tttacttcct tgaggagaat gaaccaattt
     7261 attacgtttt tctatgaaat ttttaatttc ctttcttatt tctttcggaa actcaatatt
     7321 aaagtaccct ttaaacatgc tccctaataa tttgtctaca gggggagatt gtaaattctc
     7381 aaatattaga gttaaatcag gtttctcttg ttgataaaat tcctttaccc ctatttcaca
     7441 ggctgtaatt atcgaaataa acctatctct agtatttgat atatttttac ttcttttaag
     7501 gagtataaga gaaaagggga tactttcgtg tccatgcatg taattaacta attgttgagc
     7561 ttcagaagtt atatctctaa ttatatagtt aacggaatta ggaagtgtct ttaactctaa
     7621 tgaaatttct atccaattac tatcttctaa tctgtatatg ctcgaagtat aatttgattc
     7681 gtcagcaagc atcgctacat cccaaacact gatcataaaa tcttttaaac gttcaatcat
     7741 ttcagcaata ttgctaaaat cagctaattt ttctttagaa taaaaaaaaa catttatatc
     7801 agaatcaata aattctattt tttttattac tatatcgtca tcattatctt gaccatttat
     7861 gtttattgtt ccctgagtgg ggagtacttc tttcaaattc aagttaaatt tatattccat
     7921 tacttctcct ccaatatata aaaattactt aactgtatcc aaatttaata tgtaaataaa
     7981 tcattaattt ttagctaaat tctatgagaa gtttattttt gttgtttttg ctaattaact
     8041 aaaactagta caaaaaattt aaaatagatt attaaaccta ttgcttattc tattcagtca
     8101 tatatataag cgtcatcctt tagtaactaa catcccacac tacaatagta ttataacata
     8161 tctattcaac ttcaaattgt tgaaatttaa gccatttata tttacttttc ctcaacttta
     8221 gaaatgattt tactaacctt atatccgtaa tacgcgtaac aaacacattc attaataaca
     8281 atcaacacta agctatatta gctttttaaa tgactagggt aaataaaata aggatcaaaa
     8341 acttttaagt ttctgaccct ttccttactt ccgattggtt aatagtgatc ttttgcagaa
     8401 taaataaata tcgtttcatt ttcaactcta tatatcagtc tatgttcatc tgtaattctt
     8461 ctggaccatt ttccagataa atcatgcttt aatggctcag gttttcctaa tccagcaaag
     8521 ggggaacgat cgatatcttt tattaacttg ttaatctttt ttatattgct tttgtttcct
     8581 tgctcatgcc aataaagata atcatcccaa gcatcatcag accaagcctt aatcatcaga
     8641 ttcaacctcg attaagtcat gtgttttaaa tgcacctttg gagaattgtt catctcctcg
     8701 acgaattttt tccatgacgt aattattaga aagtgttctc aacgtttctt gcatagaatc
     8761 ataatctctt tttgataata caacaactgt atcttctaca tctttacttg ttacaataag
     8821 tgtttcagca tcctcattaa cttgtttcat ataactacgt aaattttggc ggaaatttga
     8881 ataagctact gcttccattc ctttcacccc ttatttattt ctataaaaca attgtacatt
     8941 atattgtaca attaagcaaa tgattaatat tctaacttct tataattaaa agtgctatca
     9001 cttttagttt ttaggataaa aagtgacgtc actttttgag tgctggtacc tcaaatcctg
     9061 ctttttcttg ggcaatcaat aaatctaacg caatacacat aatcctggat tcacttttgc
     9121 cgtacttctg ctttagttgc tgcaattctt tttgtctctt ttcatccaac gtaacgggta
     9181 accgtttatt tcctattgca attggcatta gtactctctc ctttcatcga aataacccta
     9241 tgaaaaatat ggctgcaatc atcttgaata gtagactcac aagggctaat acaaagcgcc
     9301 aaaagatcaa acaaaatacc agtattgcca aaaatactac gaagtttaaa tgcacagctg
     9361 cagcatggcg cataagtatt tcgccaacaa ataggactaa caatagagga atgattttat
     9421 tcatatggat ctccttcttt ctcttaggtt atcgggtgtt gcccgcttat ttcgcaccac
     9481 ctaagcggcg ggggcggttg gagcgattgg tgcgaaataa gaaccattcc cttagttact
     9541 aggttttttg tccttatttc acacattctc acttttcggt ataaatgatt attgcctttt
     9601 tttcctttca aatcgttgat atggcattcg tttacggtca agttcatttt ggatttcttc
     9661 tgtatcgtcg taagtatagg tgtctaaaaa atcagttaaa gtcgtgtacc agtcgtcgcc
     9721 tgggttatct acttcttcta catattccct caaaaatgct tcgggtacag cgagttcata
     9781 atccattccc attaacacga tagattccgt tccacacgtc aaaaatctag taaatacaag
     9841 gtaataatcg tctatcgtcg catctttgct gtttagctct tccatttctt cttccattaa
     9901 ttccatgctc tcaaaaattg aataatattc tgattctgag taatagtgtt tcctgcaaat
     9961 cgtgtagctg acaaagtttt cgaccagctc actcctttct aggtaaacat tcatgatgta
    10021 tttttctgtt tctagtcgta gaagctccgt tccttttttt accgtttcag gacgacgttt
    10081 atacacgtct aaacgattat ggctgactgt gaccttcgtt tgactcgttt cactttccaa
    10141 atacaattgt tcttcgactc tttgaataaa gtacttcatt tcttccatac aactaaagtc
    10201 tttttgaatc cgtctgatta cttcattcaa atgttctttg tttgttaaaa agtgtgacac
    10261 atcttccgt
//
LOCUS       DAZUKC010000086        10107 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000086 DAZUKC010000000
VERSION     DAZUKC010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.088"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            253..522
                     /gene="rpsN"
                     /locus_tag="ACG80T_002240"
     CDS             253..522
                     /gene="rpsN"
                     /locus_tag="ACG80T_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFS4841866.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            537..716
                     /gene="rpmF"
                     /locus_tag="ACG80T_002241"
     CDS             537..716
                     /gene="rpmF"
                     /locus_tag="ACG80T_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFS4841867.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            748..1125
                     /locus_tag="ACG80T_002242"
     CDS             748..1125
                     /locus_tag="ACG80T_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841868.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1146..1295
                     /gene="rpmG"
                     /locus_tag="ACG80T_002243"
     CDS             1146..1295
                     /gene="rpmG"
                     /locus_tag="ACG80T_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS4841869.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1319..2302
                     /locus_tag="ACG80T_002244"
     CDS             1319..2302
                     /locus_tag="ACG80T_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFS4841870.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2332..2454
                     /locus_tag="ACG80T_002245"
     CDS             2332..2454
                     /locus_tag="ACG80T_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFS4841871.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2472..3995
                     /locus_tag="ACG80T_002246"
     CDS             2472..3995
                     /locus_tag="ACG80T_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFS4841872.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4019..4258
                     /locus_tag="ACG80T_002247"
     CDS             4019..4258
                     /locus_tag="ACG80T_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4841873.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4451..4912)
                     /locus_tag="ACG80T_002248"
     CDS             complement(4451..4912)
                     /locus_tag="ACG80T_002248"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS4841874.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5107..5325)
                     /locus_tag="ACG80T_002249"
     CDS             complement(5107..5325)
                     /locus_tag="ACG80T_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4841875.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5443..7059
                     /locus_tag="ACG80T_002250"
     CDS             5443..7059
                     /locus_tag="ACG80T_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFS4841876.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7300..8640)
                     /locus_tag="ACG80T_002251"
                     /pseudo
     CDS             complement(7300..8640)
                     /locus_tag="ACG80T_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9078..9224)
                     /locus_tag="ACG80T_002252"
     CDS             complement(9078..9224)
                     /locus_tag="ACG80T_002252"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841877.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            complement(9263..9457)
                     /locus_tag="ACG80T_002253"
     CDS             complement(9263..9457)
                     /locus_tag="ACG80T_002253"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4841878.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            complement(9367..9699)
                     /locus_tag="ACG80T_002254"
     CDS             complement(9367..9699)
                     /locus_tag="ACG80T_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4841879.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
BASE COUNT         3358 a         1603 c         1998 g         3148 t
ORIGIN      
        1 gtatttgtgt ttgattggac taataaaagt gaattcttat ttttcagagc ctaaactgca
       61 tattctttta aatgatttga ttatctctat gtaccgataa attcgttttc aaatagttat
      121 ctattcatat gagttttatt tcatgttatt tattgttgac aagagcaact cttgttggta
      181 aagtatcagt gtcaaaacgt aacgattacg atttgaggtg tctaaatcaa aataaaagga
      241 gtataaaggt atatggcaaa aaagtcgaaa attgctaaag caaagaaaca aatggcgatg
      301 attgaaaagt atgcagataa acgtcaggaa ttaaaagccg caggagatcg caccgctctc
      361 gctaaattgc caagagattc aaatccgaat cgtctgaggc ttcgtgatca gacggatggt
      421 cgtccaagag ggtatatgcg aaagttcggt atgtcccgta ttaagtttag agaacttgcc
      481 catcaagggt tgattcctgg agtaaaaaaa gcaagttggt aaaaggagga aatatcatgg
      541 cagtaccagc aagaaaaaca tctaaggcga agaaacggtt aagaagaact catcaaactg
      601 tagtaaaacc agaaatttca tttgacgaag cgaatggtga ttatagacgt agccatcatg
      661 tatctttaaa agggtactat aatggaaaga aagtaattaa aggagcttca aaatagttta
      721 atatataact tttaggaggg aatcaaaatg gaaattattt acccaccttt agttgaagaa
      781 ggattaaagt attatcttga aacgacccag caatcgctag ataaatgcac tttttatcgt
      841 tcgatggtag agagaggaat tattactgaa acgggtttac cgacccaaca ggcaattgaa
      901 aatggattag tcaaagatta ctatgaggac caaggcttat catttgatga ttttttaaga
      961 atctatccaa tttttgaaga gtatgacgag gagttatttc aatgtatcga tggttattgg
     1021 gagatacctg ttgatatgaa agaaaattta gtttcacaat tggaatctgg cgagttaact
     1081 tttgaagatg cacaacaaat tcaagcatat cttgaagatc gataagtatt gaaggaggaa
     1141 gatttatgcg tcaaaatatt attttggaat gtgtcgaaac tggagaacgt ctgtatctca
     1201 ccagcaaaaa taaacggaat aaccctgaac ggttagaatt aaaaaagtat tcgccaaaat
     1261 tgcgcagacg ggcaattttt aaagaagtga aatagctaag tggtaaaaag gtgacaaaat
     1321 gggcatacca gttacaatcg tttctgggtt tttaggggct ggaaaaacaa cgctaatcaa
     1381 tcaagccttg caagtctcac cctaccccag ggaagaaatt attataattg aaaatgagtt
     1441 tggtgaagtt gggattgatc acaaattatt agttcatagc gaggaacggg tgttacaact
     1501 aaataatgga tgtatgtgtt gcagtcttcg cagtgattta ttagcaatat tgatttcttt
     1561 actggaaaca atagaagaac atcaacaacc aatttcacaa atcatcattg aaactacagg
     1621 tatagcagat ccccaaccaa tcattcaaac acttttaaca gcgcctcaac taagaggaag
     1681 agtatatatc gatagtttat tgactgttat tgatagtgac agttttgaac ttattgaaag
     1741 agaaccacaa gcccttaagc aacttgtcat ggcagatcgc atttttgtgt ctagaaaaca
     1801 aaacttgaat ttatctgcac taaaaaaatt acagagagaa atcaaaagta ttaacccttt
     1861 atccgatatt cgaatgttca cacatagtga aacgattcaa aaaaaggatt tttataattt
     1921 gaaaaaattt aatcatccct tagtttttga aagtgattca tcagaaaatc actataatgg
     1981 caaatactat catcaacacc atcaagtaca tgagtttaaa tcaattctct taacttcaga
     2041 tcaagacata aaaaaagatt ttttactcca atggattgac tggctgattt taaccaacca
     2101 agggagcctg tatcgtttta aaggattgat tgctttacaa gagcaggatt ttatagttgc
     2161 catgcaagag gtaaacgaac aggttaattt tcaatacacc actcgacaaa aagaccaaac
     2221 cactattatt ctaatcggaa aaatgttgga tgaagaaagg atcaaacaga gttttgataa
     2281 attactggcg gaaagcaaat aatgataagt agtgattggg ggtaatttct aatggagaaa
     2341 acggatatat ctagtgcata tcgacgactt aaaagcccga atattaaaac tagaaaaagg
     2401 gcattaaaaa ttatcaaaga acataaacga aataaaatga agaaattagc ataattaagg
     2461 aaggaaaaag aatgaagaaa atcagtgtag gacttattgg gatagcagct ctaggattat
     2521 tgggagcttg ctccagcact aatgatgcaa aagtatcgaa tgataaagat ggaaaattag
     2581 aaattgtaac aacattctat ccaatgtatg atttcaccaa aaacatcgtc ggcgatgaag
     2641 cgaacgtgga tttaatggtt ccagcgggat cagaacctca tgactatgag ccctcagcta
     2701 aggatatggc aaaagctcat gatgctgatg tttttgtata tcataacgaa aacatggaat
     2761 catgggtacc aaaagctaaa gaaagttgga agaaagctgg gcctaatgta gtagaaggaa
     2821 caaaagacat gattttattg ccaggtagtg aagaagaaga tcatgatcac ggagaagaag
     2881 atcaccatca tgaactagat cctcatacgt gggtttcacc caaaatggca attaaggaag
     2941 tttcaaatat caaggatcag ttagttaaat tgtatccgaa aaaagcaaaa gtatttgaga
     3001 caaatgctga aaagtatttg actaaactga aacggttgga tgctgactat accactagtc
     3061 ttaaagaagc gaaacaaaaa agttttgtga cgcaacacgc agcctttgga tatttagctt
     3121 tggattatgg attgaaacaa gttcctattg ctggactaag tcctgaagaa gagccatctt
     3181 ctggtcgatt agctgaatta aaagagtatg ttaaaaaaaa taagatcaat tatatttact
     3241 ttgaaaagaa tgctaatgat aaaattgcaa aaactttggc caacgaagct gggataaaat
     3301 tggaagtact gaatcctctt gaaagtctta caaaagaaca aatggataat ggtgaagatt
     3361 atgtttcagt aatggaagac aatctaaaag cgttagaaaa aacaactatg gttgctggtg
     3421 aagaggtagt tccagaaaaa gaagcgaagg atgaaaagac agttgctaat gggtacttta
     3481 aagatgccga tgtgaaggat cgcgaattga gtgattatac aggcgaatgg caatctgttt
     3541 atccgttgtt aaaagatgga attttggatg aagttttcga ttacaaagca aaaatcaata
     3601 aagacatgac agctgccgag tacaaagatt attatactac tggctataag acagatattg
     3661 atactatcaa cattaaagac aacaccattg attttgtagt caacggtgaa catcatcaat
     3721 acacatacaa atatgttgga tacaaaattt tgaattatga aaaaggaaac cgtggtgttc
     3781 gttttaattt tgaaactgat gatgcaggtg ctggtcgttt taaatatatt caatttagtg
     3841 atcatggaat tgcaccaagt aaggcagagc atttccatat tttctttggt ggagaaagtc
     3901 aagaaaaact ctataatgaa ttgcataatt ggcctacttt ctatccagct tcattgtcta
     3961 aacatgaaat tgctcaagaa atgatggctc attaaaattc aatttttgga ggaaatacat
     4021 ggtaagaaaa gtattattgt ctatggattc caagaatatt gatgcttatc ggcgttatcg
     4081 atttgcaaag caaatgagat taaatgggag tacattagct gatattgctg caactttaca
     4141 tgttagtatt gacacagttc atagagtatt aaaacgggat cctgattgtt gttttatcaa
     4201 ggtcaatact ggaagaagta gacgtttagc aaaacggcat aatagcttgg ccaattaatt
     4261 gataatacca gagtagatca tttttccctt atttggattt gatctgcttt ttattatgtc
     4321 ttgaattgtc aaatcaagcg caacacttta tcaaagaaga cttcataagg tgttctccaa
     4381 tctaaacact ttctaggtct gttattcagt ttttcaatca tctgatttat cgttgagtta
     4441 tctaccgtag ctatctcttt cgattttggc aagtattctc gaatgagtct attcgtattt
     4501 taatttgttc attgttgcca agggcatgag gatctggaaa ataaaagggc atattattca
     4561 atgcttctgt aactgcgaaa tgattggaga attcttttcc tctatctgga gtgatcgttt
     4621 tgaccgcctc atttggaata ctgcttaaaa gttcaatcat tttctcgctc acataagcgg
     4681 ctttctttct ggggaccttc tttcctgcta aaaggaagca tgattttctg tctgtcaaag
     4741 tcactaaaca agtaccacca gttttgccag ctacaatatc ggctttccaa tgaccaaaat
     4801 cacttctatc atctgcggat tttggacggc catggatcga atgagtaagg caaatctttc
     4861 cacgagactc gacatgattt tttgtatgtc ttgtttttcc tcgatggtgc aatgaacgga
     4921 tcaggccttt attgccgtgt gagaggggtt ctgtatcgaa aagatcattg caaattgcac
     4981 gttagatcgt gataaaacta attgaaaagt ccatatgttc atgcttcaaa cgattcgaga
     5041 tttcctctgg cgaccactga aaatcgacaa ataaatgacg aacaattgac caaactcacg
     5101 gatcatttag aatgtgataa cgtccgcatt tttttcttcg ataagcataa tttttttccg
     5161 ctttggaagg tgaatagttt ttttcttgtt tcttctcagt tcatgtaaaa tagtaaacgg
     5221 gctatgctga atgtcgttgc caattatact aatagaaaat ccttcttcgt gaagcagaaa
     5281 gattctttct cgttcgagta tagtaagatg acgatactgg ctcataatta ttttccttct
     5341 aggagtgttc tcgacaaact tatactagtt ggatatcgat tatgactcat ttttatgtgt
     5401 tgcacttaaa gtgtaaattc aaggtataga aagaggttcc ctatgaaaaa aatacctatt
     5461 acaatcttaa ctggctatct tggttcagga aaaacgatac ttctaaatca tattctgtac
     5521 ggtgatcacg gaaaggaaat tgctgtcatt gtaaatgaca taggggaagt aaatgttgat
     5581 ggggtattag tcgacaagaa aggttttaaa agaactggag aaaaacttgt ttcgatgccg
     5641 aatggatgca tttgttgtac attacgcgaa gatttgattg ttgaactaga tcaattagtg
     5701 caattttctg acttgaatca aattgttatt gaagcttcag gaatcagcaa accaattcca
     5761 attattcaag caattatatt agctaaaaac cctttagaca ttgatttgac ccaacgatta
     5821 gagattggtg ccattattat agtagtggat gtaaaacgtt tttgggataa tttcgaaacg
     5881 ggtgaaaaga ttcaggaaag aaaagctgaa ggtaatgtag ttgaagaaga acaggatatt
     5941 cgtggcttgt taattgatca attcgagtgt tgtaacatat tacttttaaa taatagtgac
     6001 ttagtgtcaa ccaagacgat ttacaaaatt aacggtctaa ttgctaagtt acagtccaat
     6061 gccaaaataa ttaggacaat taaaggtgaa gtagacttat cgaaaattct aaatacaaat
     6121 ctttttaata tagatgagac ttcctattca gtgggctggt tcaaagagtt agagcttggc
     6181 tatcaaaatc atattcctga aacagaagaa tatggtatct caagttttat ctatcgaaat
     6241 cggttactcc ttgacgcaca aaaatttgcc catcttacgg ataaaaattt tcctggcaat
     6301 attacaagag caaaagggtt tctttggttt gcccaacata gtgatttttc gacgatcttt
     6361 gaaacatcgg gtagctataa aagtattgaa tcattaaatt attgggtagc tagtttacct
     6421 aaaattgagt gtcaaatgat tatacaaaaa aatcccgaat tgaaggaagc ctggcatccg
     6481 ttgtttggtg aacgcattaa taaactagtt tttattggta tgaagatgga tcaacaatgt
     6541 attaaggaac aacttgaggg ttgtttaact gattctctta atgcagataa tttccgaaaa
     6601 atttcaccat ttccatggaa aaagtctaat atgtttagcc agatatcgtt aatgaaattt
     6661 ttaagaaaag ctggtgtcaa gataggcgat gtcataatta ttggcatttt gattattacc
     6721 tcttttattc ccttatttgt attctctata gaaaaggaga atgaaacaac tgaagatgct
     6781 atcaaatatg ccatcgttcg aattgatggc aaggaaatgg atcgcttcga tttggataag
     6841 atcaatcata aactcgtgaa ttatcatcct gcgaagggac aatataatat tgttgagatt
     6901 aaagatggaa gaattcgagt taaagaagat aatagtcctg atcaaatcgc ggttagaact
     6961 ggatggatct caaaacccgg tcaaacgagt atttgtttac cccataagtt agttattagt
     7021 attgagaaaa aagaaagcac agattactac atttactaat ctggaaatag atgaggaggt
     7081 ttttaataag aaaaagatat tattattaat aatagcaggg gctttattaa gtgtgttaag
     7141 cgcttgtgga caagaaaaag aagcggtgac aacaggcaca agtatcttta acgggacctg
     7201 caatggttta gttaacagtc ggttagatta cttattttgg ctcttcgtca atgagtgttg
     7261 gtgaagaaat tcaatagaat aatcgagata ctcacacggc tagacagcta tcttgtctag
     7321 ccgtgttgtt gttctggctt tagtcagttg ggtagtattc ttagttttct taaaatattt
     7381 tcctcgatat aaaaagattc gttctcggta gttttgaaag tttctatagc cataggcaat
     7441 tcgttttagt actttgatat ggttattcaa acattctata gggccgttgg aatagggaac
     7501 gcgcaacgct cgtttgattt ctgtttggta tttcttgaag gtcgtgaaca ctggttggta
     7561 gtgctctggc aactccttgt agtcttcttt taggagcgcg acgaattcag gaacatcttg
     7621 tcttctgata acggaaagaa aatcctggta gacttcgtag cctcgcttta aggaatcatc
     7681 ataagataat agccgatcga ctatctctgc ttctgtcaga tgatctcgaa atgacggacg
     7741 ccagtagcga tgctgacctt tcaattccca cgcattcttt tgaagtagtt tccagtattt
     7801 ctttaagtgc ttcgaccgac gatccccttt tttaaaggtc ttcattactt gaattcggtg
     7861 gttggtaaaa gcccgaccaa tgtgttggct aatatgaaag cgatcggcga cgattcgggc
     7921 attcggaaaa cactctttca ccaatgtgcg atagggtgta tagaaatctg aaaccactag
     7981 acgcacttgt tcacggactt ttatagggta gcgcaagaaa tagtcgcgta aaggattgag
     8041 ttgccgattt tccacaatat caatcagttg atgggtcttg ccgtccatca tgacaaagct
     8101 catggctccc gagacatttt tcactgattt gaattcatcg aaacacacca cttctggtaa
     8161 cagctggctc aaagaaatgg ttttaggccg aaggctgcga agtattcgaa tgacagtggt
     8221 cggagaaatg tgcttcatac gtgcgatcaa agacatagaa agaggttcgc ttaatagctc
     8281 aagaatggct tgtttgactc tgcgagcaat ggagcagcga cgatcactaa ctgaatcttc
     8341 tgctaaaaaa gtctttccac agtgtttaca tttgaagcgc tgtttgttaa tccgcagaat
     8401 ggtcttgtag ttactgacat cattcaataa gatggatact tttttccagc cctatttaat
     8461 gatcgtttgt tcattttgat agtggcgatg aaagcattcc tccggttgat aggtcaatgt
     8521 gccatcaaaa acaaaacatg tttcgtcatt gattcgctct ttgcgaaagg catcttcatg
     8581 aaagataaga tttaggtcta gaatatctag taactctttg gtaaactctg aataggacat
     8641 cttgaaacac tcctttgaat ggttttcgtc gactttattc taaaggattt taaggtgtct
     8701 gtttgcgttt attagaaaaa agaaaaagta ttggcaacag aaggcgtcac caacactaga
     8761 tattatagat cccttatttt cttcgcttca aattgtcaag gagtcatcat aaattttgta
     8821 tggattcttt tgtttgggta gtagtcttct aaataatctt ggattaacac cttcatctct
     8881 attttacttt tatatttgtt ggagtcgatt aattctcatt tgattgatcg atgaaacgct
     8941 tcaattactg cattgtcaac aggagtccca gtttggctca ctgaatgtgt gatgtggtgg
     9001 tcgttcaaca gctcttgata aataaaagac cgatgctggc tacctctatc ggaatgaatt
     9061 accgtaggca attctcctta ataaatttgt aatggacgga cgacaatctc ttttttcatg
     9121 tgtgaatcta ccatatagta acgtagaata cgagttgcta agtcaatata ggtggacaaa
     9181 tagaggcgac cgtcattaca tggaatgtaa gtaatatcca tcacccaaac agcatcaatt
     9241 tggttctgtt caaattatcg attcaacatt tttttaccat ttctttgatt gttttttctc
     9301 taccatgaga agatttgcgt cgatgaaatc ctcttgtgca taaattcatt tcacgcataa
     9361 gaacagctac aacacgttta ttgatttcga ttccttcatc gtaaagtttt tgagtgatcc
     9421 ctggagatcc gtatcgatct ttgtgttcgt agaacactcg tcttcctctt ttagtcgttt
     9481 attttcctgt tcaagaatgt agaaatctgc tttatcaggt aatctttttc cttgtccagc
     9541 aaaagcattt ttcccgtatc gacgatattc tgaaacccat cgggaaattg ttggacgaga
     9601 gatattgtat tcttcagcaa ttttcttggt agttactttt ttctcaagga tatcctgtga
     9661 aatctttagt ttaaatttac gatcatatgt ttttctcaat agagttcatt ccttccggta
     9721 acgtaactga tagtcaagaa aagtgtatca tagcccaact tagtatcagg gtttgtcaac
     9781 taaactgtgg aagttgatag ataagagttt ttaacaacca aaattctctt gactattttt
     9841 gaaatcagac aaattttgat tggacacata gttgaataaa tctaccgtcg ttacggaagg
     9901 taaatcgcat gcttttcaat tctagaggag aagtcctcgt tgaagtacca tagcccagtt
     9961 ctggattcga cctcctgccc acttcatacg taaataaagt aattttagga gtgagactgt
    10021 gacaaaagtt atttttaagc taaaaacgaa ttaaaaaacg ttgataatta aaatcattcg
    10081 ttttagattc tatgaatcat tatagaa
//
LOCUS       DAZUKC010000087         9955 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000087 DAZUKC010000000
VERSION     DAZUKC010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.089"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..439)
                     /locus_tag="ACG80T_002255"
     CDS             complement(<1..439)
                     /locus_tag="ACG80T_002255"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4841880.1"
                     /translation="MEEFGIDSISRTEALDFQADYEYELDQITMIPEIHQLLLALHKN
                     RIPIGILTNGPVDLQSRKLQNIGAYQYFEKQNIIISQSTHGGAAENSYEGNVKNLQSY
                     QNVQLSKRAIEILKEEIELNHQHIRFNPDYKDNGWIFTSKSRHK"
     gene            complement(662..1429)
                     /locus_tag="ACG80T_002256"
     CDS             complement(662..1429)
                     /locus_tag="ACG80T_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS4841881.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            1917..2342
                     /gene="lacA"
                     /locus_tag="ACG80T_002257"
     CDS             1917..2342
                     /gene="lacA"
                     /locus_tag="ACG80T_002257"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HFS4841882.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            2359..2874
                     /gene="lacB"
                     /locus_tag="ACG80T_002258"
     CDS             2359..2874
                     /gene="lacB"
                     /locus_tag="ACG80T_002258"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HFS4841883.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            2885..3817
                     /gene="lacC"
                     /locus_tag="ACG80T_002259"
     CDS             2885..3817
                     /gene="lacC"
                     /locus_tag="ACG80T_002259"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009024 - tagatose-6-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HFS4841884.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTVSHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            3820..4800
                     /gene="lacD"
                     /locus_tag="ACG80T_002260"
     CDS             3820..4800
                     /gene="lacD"
                     /locus_tag="ACG80T_002260"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFS4841885.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            4828..5145
                     /locus_tag="ACG80T_002261"
     CDS             4828..5145
                     /locus_tag="ACG80T_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS4841886.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            5145..6851
                     /locus_tag="ACG80T_002262"
     CDS             5145..6851
                     /locus_tag="ACG80T_002262"
                     /EC_number="2.7.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0019197 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase complex [Evidence IEA];
                     GO_function: GO:0015155 - lactose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactose-specific PTS transporter subunit EIIC"
                     /protein_id="HFS4841887.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTVLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            6993..8399
                     /gene="lacG"
                     /locus_tag="ACG80T_002263"
     CDS             6993..8399
                     /gene="lacG"
                     /locus_tag="ACG80T_002263"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFS4841888.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            8422..8694
                     /locus_tag="ACG80T_002264"
     CDS             8422..8694
                     /locus_tag="ACG80T_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HFS4841889.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            8715..9614
                     /locus_tag="ACG80T_002265"
     CDS             8715..9614
                     /locus_tag="ACG80T_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFS4841890.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         3138 a         1678 c         1996 g         3143 t
ORIGIN      
        1 gtttatgacg acttttggaa gtaaaaatcc atccattatc tttataatca ggattgaatc
       61 ttatatgttg gtgatttaac tcaatttcct ctttaagaat ttctatagcc cttttagata
      121 attggacatt ttggtaactt tgtaagtttt ttacattacc ttcatagcta ttttctgcag
      181 ctcctccgtg agtggactga ctaatgatga tattttgctt ttcaaaatat tgataggcac
      241 caatattttg gagttttcga ctctgtaagt caacagggcc attagttaga atacctattg
      301 ggatgcggtt tttatgtaga gccagtagta gttgatgtat ctcaggaatc atcgtaatct
      361 gatcgagttc atattcatag tcagcttgga agtctagagc ttctgtccta gaaatactat
      421 cgatgccaaa ttcttccata gtcttctgac agcgaaaaat tctaagctgc tcaatactga
      481 tgagtttttc gtgtatttag gaaaagcaac atcagaccaa catctgaaac gtttgtaaat
      541 gttatctatt tgttggatat caatcgttgg aaaacatttc tcaacgctac ttttaaaggg
      601 gagttgatgg tcataaagag tgtcatcaag gtcaaaaaca atatttttat aagtcataat
      661 tctagtccgc ttttaaaata gtagtgtact ggctaaattc ctctaagtgt tgagggctaa
      721 tctggttatc tgtaatgatg gtatcgagtt gatctagatt atagaagtta aagaaatcgt
      781 atcgatcgaa tttagtactg tctactaata agaatttttc tacagcattg tttagggcaa
      841 gttgttgaat cacaccttcc ttgtcactat atgtagcaat agaattatgg gtaacagcat
      901 ttgcactaac aaaagctttg gcaaatctaa ttgcttttaa atttgtcgaa gccattgaac
      961 ctacaaaagc tccagttatt tctctatatt caccgccaag aagcaataaa tcaatggttt
     1021 cgctatcatt tagaatcaag aacacaggga gactatttgt aatgacacga attttataac
     1081 ctttacgacc tttcaattct aatgccagtt gttcaagtgt agttccgggt ccaataaaaa
     1141 tagtatcgcc atctttgatt aaagagcagg cttttttagc aatgtcaatt ttttcttttg
     1201 tatttagact tttcttctcg atatgtgtct tttcaagtgg ctttttagag gaaagtaatt
     1261 gtgcaccacc atgagtacgt gttaaaagtc catcagcttc tagagcatca aggtctctac
     1321 gggctgtcat atcggaaata tctagttcat ctattatttc ttttatggtt atggtcccat
     1381 caatctttaa catatctaaa attttttcta atcgtcgttt tttgttcata tgaagaccct
     1441 tctttcataa agtaattttt ttccaaagat aattctcttt taattgtatc ataaaagaca
     1501 atattttcaa ggtaaaacaa acaatttcaa acaaaaacaa atgttagatg atgaaataag
     1561 aacagtggat tgacgtatat tagcttaggt cagattttgt ataagacgaa aataaagtag
     1621 gacctcttaa tcagtaagtt atagaaagta aaagactttt gtaatacttg aatagatatt
     1681 tcacgtccat tttgtgatgg attaaatgaa caaaaatgaa caataattta acggtgttat
     1741 ctatttttta aaaaaacaaa taaaaaaaca aaaaataaac aaaaaagtgt tgctttttgt
     1801 ttgaatgctt gatattatat aaacaaagaa atgatgaaaa cgttttttcg aacattttgc
     1861 aaaatgtttt ctacttctac gtagcatttc tttctaaaat ttaggaggtg gtcaaaatgg
     1921 ctattgttgt tggtgcagat ctcaaaggta cgagattaaa agatgttgtg aaaaattttc
     1981 ttgtagaaga aggttttgag gtaattgatg tgactaagga cggacaagat tttgttgatg
     2041 ttaccttggc agttgcctct gaagtaaata aagatgagca aaacttaggt attgtaattg
     2101 atgcttatgg agctggtcca ttcatggtag caactaaaat taaaggtatg gttgcagcag
     2161 aagtttcaga tgaacgctca gcttatatga cacgtggaca taacaattca cgaatgatta
     2221 cggttggcgc tgaaatcgtc ggtgatgagc ttgctaaaaa catcgctaaa ggtttcgtca
     2281 atggtaaata tgatggcgga cgtcatcaaa tccgagtaga tatgcttaac aaaatgtgtt
     2341 aaggaaagga gttaaggaat gagaatcgca attggatgtg accacattgt cacagatgaa
     2401 aaaatggcag tatcagaatt cttgaaatca aaaggacatg aggtccttga tttcgggaca
     2461 tatgaccacg cacgtactca ctaccctatc tatggtaaaa aagtcggtga agcagtagta
     2521 agtggtcaag cagacttggg agtatgtatc tgtggtacag gtgtcggcat taacaatgca
     2581 gtcaataaag ttccaggtgt tcgctcagcg ttggttcgtg atatgacatc agctatatat
     2641 gcaaaagaag agttaaatgc gaatgttatc ggtttcggtg gaaagattac tggtggtctt
     2701 cttatgaatg acatcattga agctttcatt gaagctgagt acaaaccaac agaagaaaat
     2761 aaaaaattga ttgcaaaaat cgaacacgtt gaaacacata atgcacatca agcagatgaa
     2821 gatttcttta cagaattcct tgaaagatgg gaccgtggtg actaccacga ttaaggtata
     2881 acaaatgatt ctgacagtca cactaaatcc ttcagtagac atctcttatc ctctagaaac
     2941 attgaaaatt gatacagtga atagggtgaa agatgttagt aagacagctg gtggaaaagg
     3001 cttaaatgtt actagagtct tgtatgaatc tggtgataaa gttactgcta caggtttctt
     3061 aggtggtaaa attggtgaat ttattgaaag cgaattagaa caatctcctg ttagtccagc
     3121 tttttataaa atctcaggaa acactagaaa ctgtattgct atcttacacg agggtaacca
     3181 aacagaaatt ttggaacaag gaccaacagt ttctcatgaa gaagcagaag gttttcttga
     3241 ccactatagc aatcttatta aacaatcaga agttgtgaca atttcaggaa gtttaccttc
     3301 aggacttccc aacgattatt atgaaaaact tatccaactt gcttcggatg aaggagtagc
     3361 agtcgttttg gattgttcag gtgcacctct tgaaacagtt ctaaaatcgt cggctaaacc
     3421 tactgccatc aaacctaata atgaggaact ttcacagttg cttggaaaag aagtaacaaa
     3481 agatattgaa gaactcaaag acgttcttaa agagtcactt tttagtggta tcgaatggat
     3541 agtggtctca ttaggtcgaa acggtgcctt tgcaaaacat ggtgatgttt tctataaggt
     3601 agatattcct gatatcccag ttgttaatcc agtcggatca ggggactcaa cggtagctgg
     3661 tattgcatca gctttaaata gtaaaaaaag tgacgctgac ttattaaaac atgcgatgac
     3721 attgggtatg ttaaatgctc aagaaacaat gacagggcat gtgaatatga ctaattacga
     3781 aacactaaac tcacaaattg gagtaaaaga ggtataaaaa tggtacttac agaacagaaa
     3841 cgcaaatctt tggaaaaaat ttcagataaa aacggtgtta tctcagcttt ggcatttgac
     3901 caacgtggtg ctttgaaacg tttgatggca cagtatcaag atacagaacc aactgtagct
     3961 caaatggaag aactaaaggt tttggttgct gatgaactta caaaatacgc ttcttcaatg
     4021 ttgcttgacc ctgaatatgg acttccagca acaaaagcat tggataaaga agcaggtctt
     4081 cttcttgcct atgaaaaaac aggttatgat acatcaagta caaaacgttt gcctgactgc
     4141 ttggatgttt ggtctgcaaa acgtatcaaa gagcaaggcg ctgatgccgt taagttcttg
     4201 ctttactatg atgtagacag ctcagatgaa cttaaccaac aaaaacaagc ttatattgaa
     4261 cgtgttggtt ctgaatgtgt cgctgaagat attcctttct tcttggaaat ccttgcttat
     4321 gatgaagaaa tctcagatgc aggttctgtt gaatatgcaa aagtgaaacc tcgcaaagtc
     4381 attgaagcga tgaaagtttt ctctgatcca cgctttaaca ttgatgttct taaagtagaa
     4441 gtaccagtta acgttaaata tgtcgaaggt tttgctgatg gtgaagtggt ttacagtaaa
     4501 gctgaagctg ctgacttctt taaagcacaa gaagaagcga ctaaccttcc atacatctac
     4561 ctaagtgcag gtgtatctgc taaattgttc caagaaacat tgcaatttgc tcacgactca
     4621 ggtgccaagt ttaatggtgt gctttgtgga cgtgctactt gggcaggatc tgtagaacct
     4681 tacatcaaag aaggtgaaaa agcagcacgt gaatggttgc gtactactgg attcgaaaat
     4741 attgatgaac ttaacaaagt tcttgttaaa acagctagtc catggactga taaagtatag
     4801 taaaaacata aaacggagga tattgttatg aacagagaag agatgactct cttagggttt
     4861 gaaattgttg cttatgctgg agatgctcgc tctaaacttt tagaagcgct taaagcggct
     4921 gaaaatggtg atttcgctaa ggcagatagt cttgtagtag aagcaggaag ctgtattgca
     4981 gaggctcaca attctcagac agctatgttg gctcgagaag cttctgggga ggaacttcca
     5041 tacagcgtta ccatgatgca tggtcaggac cacttaatga ctactgtctt attaaaagat
     5101 gtgattcatc acctcatcga actttataaa agaggagcaa aataatgcat aaactcattg
     5161 aacttattga gaaagggaaa ccgttctttg agaaaatctc tcgaaacatc tatcttcgtg
     5221 ctattcgtga tggatttatc gctggtatgc cagtcatcct tttctcatct atctttatcc
     5281 ttattgccta tgtcccaaat gcttggggat tccactggtc aaaagacatt gagaccttct
     5341 tgatgactcc ttatagctat tcgatgggta tcctagcatt ctttgttggt ggtactaccg
     5401 ctaaagcttt gacggactca atgaaccgtg acctacctgc gaccaatcag attaacttct
     5461 tatctacgat gctagcgtct atggtagggt tccttttgat ggcagctgag cctgcaaaag
     5521 aaggaggctt cttgacagcc ttcacgggaa caaaaggtct tttgacagca tttattgcag
     5581 cctttgtgac agttaatgtt tacaaggtct gtgtgaaaaa taatgttact attcgtatgc
     5641 ctgaagaggt tccaccaaat atctctcaag tatttaaaga cttgattcca ttcactgtag
     5701 cggttgttct cctttatgga tttgaactca ttgttaaggg aactcttggt gtaaccgttg
     5761 cagaatcaat cggtaccgtt cttgctcctc tcttctcagc tgcagatggt taccttggaa
     5821 ttacacttat ctttggtgct tatgccttct tctggtttgt tggtatccac ggtccttcaa
     5881 ttgtcgaacc agcaatcgct gctatcactt atgccaatat cgatgccaac ttgcatctta
     5941 tccaagctgg acaacatgca gataaagtta tcacttctgg tactcaaatg tttattgtta
     6001 ccatgggtgg aacaggagct acattgattg ttccattctt gttcatgtgg atttgtaaat
     6061 cagaacgtaa ccgtgccatc ggacgtgcct cagttgttcc aacattcttt ggggttaatg
     6121 agccaatctt atttggtgca ccaatcgtat tgaatccgat tttctttgta ccgttcattt
     6181 tcgctccgat tgtcaacgtt tggatcttta aattctttgt tgacacattg aacatgaact
     6241 cattctctgc caaccttcca tgggttactc ctggtccgtt gggaattgta ctcggtacta
     6301 acttccaagt tctatcattt atcttagccg gactcttggt agttgttgat actatcattt
     6361 attacccatt tgttaaggta tacgatgaac aaattcttga agaagaacgt tctggtaaaa
     6421 caaacgatgc tcttaaagaa aaagtagctg caaacttcaa tactgctaaa gcagatgccg
     6481 ttcttggaaa agcaggcgtt gctaaagaag atgtagcagc aaacaataat atcacaaaag
     6541 aaactaatgt ccttgttctt tgtgcaggtg gaggtacaag tggcttgctt gccaatgcct
     6601 tgaacaaagc agcagcagaa tacaatgttc cagtcaaagc ggcagcgggt agttacggtg
     6661 ctcaccgtga aatgttgcca gagtttgact tggttatctt ggctccacag gtcgcttcaa
     6721 acttcgatga tatgaaggct gaaacggata aattgggcat taaacttgca aaaactgaag
     6781 gagctcaata tatcaaattg acacgtgatg gacaaggtgc tcttgcattt gttcaacaac
     6841 agtttgatta ataaatttgt aaaagttatt tgagacgaga ttattctttc agttccttat
     6901 aatattgtta accatatcat tgcatttcat ttccttggtt tccggaactc agaatatcgt
     6961 ctcaaatgct ttttttgaaa ggatttatag ttatgactaa aacacttcct aaagatttta
     7021 tttttggtgg tgctacagct gcttatcaag cagaaggtgc gactcacacg gatggtaaag
     7081 gcccagttgc atgggataag tatcttgaag acaattattg gtacacagca gagcctgcaa
     7141 gtgattttta ccataaatat cctgttgatt tagaattagc tgaaaaatat ggcgttaatg
     7201 gtattcgtat ttcaattgcc tggtctcgta ttttcccaac tggctatggc gaagtaaatg
     7261 aaaaaggtgt tgaattctac cataatcttt ttgccgagtg ccacaaacgt catgttgagc
     7321 cattcgtgac tttgcaccac tttgacacac cagaagttct tcactcagat ggagatttct
     7381 taaaccgcga aaatatagaa cactttgtag attatgccgc tttctgtttt gaagagttcc
     7441 cagaagtaaa ctactggaca actttcaatg aaattggccc aattggagat ggtcaatact
     7501 tggttggtaa attccctcca ggcattcaat atgatcttgc caaagtcttc caatcacacc
     7561 acaatatgat ggtttctcat gcacgtgctg tgaaattgta taaagataaa ggttataaag
     7621 gcgaaattgg tgtagtacat gcgttgccaa ccaaataccc ttatgatcca gaaaatccag
     7681 cagatgttcg tgccgctgag ttagaagata tcatccataa caaatttatc ttggatgcaa
     7741 cctaccttgg acactattca gataaaacga tggaaggggt caaccacatc ctagctgaaa
     7801 atggtggaga acttgatctt cgtgatgaag acttccaagc acttgaagca gctaaagatt
     7861 tgaacgattt ccttggtatc aactactaca tgagtgattg gatgcaagcc tttgatggtg
     7921 agactgaaat cattcacaat ggtaagggtg aaaaaggaag ttctaagtac cagattaaag
     7981 gtgttggtcg ccgaatcgca ccagattatg tgcctagaac ggattgggac tggattattt
     8041 atcctgaagg cttgtatgac caaatcatgc gagtgaaaaa tgattatcca aactacaaga
     8101 agatttacat cactgaaaac ggtcttggat acaaagatga gtttgtagat aataccgttt
     8161 atgatgatgg tcgtattgat tacgtgaaga aacacttgga agttttatca gacgcaattg
     8221 cggatggtgc aaatgttaaa ggttacttca tctggtcact tatggatgtc ttctcatggt
     8281 caaacggata cgaaaaacgt tatggactat tctatgtaga ctttgacacg caagaacgct
     8341 atcctaagaa atcagctcat tggtataaga aagtagcaga aactcaagtg atagagtaat
     8401 attacttgaa aatgtaaaaa attgttatcc gatactttag aaaaagtaca acacttattt
     8461 agaccaggct ttgaaaaggt atatattgtt agtttcgaag attgtcctat gattcctgag
     8521 cttgaagcta ccccgttgct aaaatgtggt aaatggtatg tttcaactgg aaaagagtgg
     8581 atttgccatt ctgatttaga gttatcagca tttgaatggg aatttttaca atcactcgat
     8641 gtagaaatac gtgagactat tcacttcgag gtaaattatt tgcctttcca ataataaaaa
     8701 gaaaggaatt agttgtgact atcgaattaa aaaacgagta tcttactgtt cagtttaaaa
     8761 cacttggtgg tcaactgact tcgattaaag acaaggatgg gatagagtat ctctggcaag
     8821 ctgatccaaa ttattggaat ggacaggcgc ctattctatt tcctatctgt ggtagtttac
     8881 gaaatgattg ggctatttat agacctcaag aaagaccttt ttttacaggt cttattcgta
     8941 gacatggttt tgttcgaaaa gaagaattta ttctggaaga agtcaatgat aatagcgtga
     9001 cctttagtat taaaccaaat gctgagatgc ttgataatta cctatatcag tttgaactga
     9061 gagttgttta taccttgaat ggtaaatcaa ttagaaccga atttcaggta acgaacttgg
     9121 aaatggaaaa aacaatgcct tactttattg gagcccatcc agcattcaat agtcctttag
     9181 tagaaggtga aaactatgag gattattata ttgaatttag tgaggttgag tcttgctcga
     9241 taccaaagag ttttcctgag acaggattat tagatttgca agatcggaca ccttttttgg
     9301 aaaatcaaaa aattctcgat ttggactact cacttttttc ccatgatgcc attacacttg
     9361 accagctcaa gtctcgtagc gtaacgcttc ggtcaaggaa gtctggtaaa ggtcttcgag
     9421 tagattttga tgattttccg aacttgattc tgtggtctac aactaataaa agtccattca
     9481 ttgctttgga accttggagt ggtctctcca cttcacttga agaaggtaat ttcttagaag
     9541 ataaaagaca ggtaacgaaa atttcacctc aagaaacttc tagaaaaatc tatgatatta
     9601 ctatccttaa ttaaaatttg ttattatttg agtcttcaat aagatttgaa tactcttacc
     9661 ttgtgatgat agattggtct tctccgtgcc agtcttacat cgcaaggttt ttaagttttt
     9721 tgtgggtcta tgtcaactgt agtgggtgac gaaaagctaa tatctaaaga cagtctggta
     9781 gtaaaaaata agattattta ctacttcttg atttaaaata atagtggagc cattaatttg
     9841 tggcaaattt aatttaatat tttgcctcaa attagccaca ctttttatag aactaagagg
     9901 aaaaatgaaa ttttcttcct attaatatag ttgtttgaaa tcttaaatat agcgt
//
LOCUS       DAZUKC010000088         9887 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000088 DAZUKC010000000
VERSION     DAZUKC010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.090"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..221)
                     /locus_tag="ACG80T_002266"
     CDS             complement(<1..221)
                     /locus_tag="ACG80T_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS4841891.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQILKVKEK"
     gene            422..1636
                     /locus_tag="ACG80T_002267"
                     /pseudo
     CDS             422..1636
                     /locus_tag="ACG80T_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1815..2150)
                     /locus_tag="ACG80T_002268"
     CDS             complement(1815..2150)
                     /locus_tag="ACG80T_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS4841892.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(2134..2397)
                     /locus_tag="ACG80T_002269"
     CDS             complement(2134..2397)
                     /locus_tag="ACG80T_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFS4841893.1"
                     /translation="MAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQGKI
                     PFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2857..3660)
                     /locus_tag="ACG80T_002270"
     CDS             complement(2857..3660)
                     /locus_tag="ACG80T_002270"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS4841894.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3638..4762)
                     /locus_tag="ACG80T_002271"
     CDS             complement(3638..4762)
                     /locus_tag="ACG80T_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4841895.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4778..5593)
                     /locus_tag="ACG80T_002272"
     CDS             complement(4778..5593)
                     /locus_tag="ACG80T_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4841896.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5583..6455)
                     /locus_tag="ACG80T_002273"
     CDS             complement(5583..6455)
                     /locus_tag="ACG80T_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4841897.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6540..7829)
                     /locus_tag="ACG80T_002274"
     CDS             complement(6540..7829)
                     /locus_tag="ACG80T_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4841898.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7973..8485)
                     /locus_tag="ACG80T_002275"
     CDS             complement(7973..8485)
                     /locus_tag="ACG80T_002275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841899.1"
                     /translation="MTINCDLKEEGLEMAVFSLAQTYQIWPQIVLSGYVQLSWAAIWG
                     KHIFYNIENQFEGKKLGDITEADLIDTLQKLAYQSVQIVNIHEGWLTKLAREVGQQLG
                     LTFSVWTVNELETIEYLARCGVYNITSRCAWAYLWKKGAQNKLFYNQNRGVKRFGDGI
                     HDHMGALFLA"
     gene            complement(8552..8695)
                     /locus_tag="ACG80T_002276"
     CDS             complement(8552..8695)
                     /locus_tag="ACG80T_002276"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFS4841900.1"
                     /translation="MITAHSGSDNTEENSWAFLRFCLDRQLCFEVDVRQQTDGTLILA
                     HDR"
     gene            complement(9178..>9887)
                     /locus_tag="ACG80T_002277"
     CDS             complement(9178..>9887)
                     /locus_tag="ACG80T_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1182-like element ISEfa7 family transposase"
                     /protein_id="HFS4841901.1"
                     /translation="LVKQSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRP
                     KGKKGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNCPLLNQC
                     TISKNHQRVITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKHGMRWT
                     KYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQARVTSLE
                     QLLSTVWLSVRTAFFVG"
BASE COUNT         3043 a         2092 c         1594 g         3158 t
ORIGIN      
        1 aacttttctt tgactttcaa tatctgagtg cgtgtcgtat aggaaccgtt gcggataata
       61 cgaaccgatc gacagtgttc acaataggaa ggagagtcat ccaattttcc tttaataaac
      121 tgcgcttgaa cgcctttata ttctttttca atcagccatt cttcatcaaa aaataaatgt
      181 ttatctgtta atccgagtaa ttttctagta tgattatcca ttgagagact cctttggtag
      241 gatgacgttt tggtcgacta aattttacca aaagtgagtc tctttttgag ttgaaacgaa
      301 gaaaaagtgt tagtagaaaa tcgccttcga ttttccacca acacaaaata ttatagaagc
      361 aaaatcaatt ctaccttaga tgttcttata tcactctatc agtcctattt gacaaagagc
      421 caagaaaaag acgttggtcg tcgatgctgt tctctcgcct actcctcgtg cttgtagaag
      481 ttgtggttct actgtggtag atggaaacgg gaaagcaatt atagtgaaaa atgggaagaa
      541 ggaaacgatt gtccgttttg aacaatacaa tcatatgcct ttggttacgc gcctaaaaaa
      601 gcaacgctat acctgtaaaa actgtcgtac ccattggacg gctcaaagtt attttgtccg
      661 actcagccat tcaatcgcag atcatgttcg atataaaatt gcttctttac tgactgaaaa
      721 agtatctttg tcttttattg cgaaaagttg tcaggtatct ttgactaccg ttactcgtac
      781 attgaaagag tttaaaagct atttaccaaa gccttctaag aagattctcc ccagagtatt
      841 gatggttgat gaatttcgtt cgcacgcttc catagaagat aaaatgagct ttatttgcgc
      901 agatggtgaa acaggaaact taattgctgt cttgcctacg cgtaaattac ctcgattaac
      961 aagctatttc ttaggctgca ccaatccaga agaagtagaa ttcttagtca cagacatgaa
     1021 cgccgcttac ttccagctta ccaaacgtgt tctgccaaat gcgaaagtag tgattgatcg
     1081 gtttcatatt gtcaaacaca tgaatcaggc actcaatgag ttgcgtatcc gtgaaatgaa
     1141 tgagcttcgt aaagcaggac agaaaagtca ggcagaaaaa ctgaaaaaga actggcgctt
     1201 tctgctaaaa aatcgtgcaa gcatcaacca ttatgagtac aaaacatgga aaagtttccg
     1261 agcaccaaaa tacccatttc tcactgaagc aatgatgatt gatcgattgc ttgagttttc
     1321 tgcgcctcta aaggaggcgt atcccttttt tcatgaatta gtcgaagcct ttcgagacaa
     1381 agaccctgac ttatttttct ccttattggc agaacttccc gaaacgttag atgacggctt
     1441 tcgggagaag cttcaaaacc ttctgaccta tgaagaaggt atcaccagcg caatgatcta
     1501 tccttattcc aatggaaaaa ttgaagcgaa gaatacccac ataaagacaa tgaaacgagt
     1561 atcctacgga tttaaatcat tcgagaacat gagaattaga atctttttga tcaatcaatt
     1621 aatcaatgta agataacaaa aaatctgaac cagaatgtgg ttcattctga cccggatttg
     1681 attttgcaca actcatcagt ccttattgac aaagagccaa aaatctgagc cagaatgagg
     1741 ttcattctga cccagatttg attttgtaca acccatcagt ccttattgac aaagagccct
     1801 tctttttagt attattataa ttcttccatg cgttcttcca gtcgctgtaa atcgcttgga
     1861 cttaattccc caattttttt ccatttgacc tttttgttgt ttttgggtaa ttcgtttaga
     1921 gttcctacgt caatccaaga ttcttttttt agtcctgcct gtttccaatc ccttatttca
     1981 acgtatttca gctttatttt atcggattta gtatgatatt ttgaagtaat tgacagaaac
     2041 gaaattgtgt ccagtccatt ttttagaata tagacaggcc ggcgtttacc gccctctgca
     2101 gctgtgaaag caacgtagac ggttgctata tcattagtat tcatcttcat tgaaccattc
     2161 ttcaattttt tcgtcagtgt ctagtttttg gacaggcaat ttttccgcta atctttgaat
     2221 attatggatt cgtttacttt tttccgacaa ttcaatagaa aatgggattt ttccttgctc
     2281 aacaatttgc ttataaaaaa cggtgacggc tgttttgggg ctcatgccta gttcatcaat
     2341 aatgctttct gcttcatttt tcaatttttc gtccatagaa atttgtaact tagccatttt
     2401 ctccacctct ttctgacaaa cacattatat cacggaaaga gttcaaatgg aattaataca
     2461 actacttttg aactccttga aatgcagtgt atttatctaa atataaggaa aggatatagt
     2521 gctaaatggc cttgatagat agaaaagttt caaaatccgc cacagcgata attgaagcga
     2581 acactcaacg acacacagaa caggaaaaga ctcctaaaag aaccaaatta actaggacgt
     2641 gtctgaaaac tccacaaaga tgtggtatgc tctggtaaaa aacgaatggc acaaccacga
     2701 agaccgatat ggttcttgga aaactttctc cacccttttt gtaattggag ggatagtggt
     2761 ttattgatag cgattttcca agttcttcaa gtggaaccag acattgagca ccacggctaa
     2821 aaagtgaatg aagtctttat ttttaatcaa taatttttaa tgattaccag taattggttt
     2881 tttcatgcgc cctatttcta tttgatgtgg agttaaataa tgctgataag taaaaatttt
     2941 tccgtcaatt aaactgccac tagtgaatcc tgttctattg gtaaaaagag aatagtctgg
     3001 gtattcatct acaccaaatg ataaagattc agccataaac tgttttttac cagcaaattc
     3061 agcactgcta ccttgatgga tatgtcctac aaaaatacct aaaatatcac tttcggcaat
     3121 caattcagca aaaccttccg gaacttcagt tacaatagat ggatcttccc acgacaatgg
     3181 atgatgaacc aataaaatcg ttcccttttc agcaggtgta cccaaccact cttgtaattg
     3241 aatcatctgt cctatcgata tttttccttc gtgctgggta tcttgagcag aatccaagga
     3301 aataattcgt aacccatttt gattcgtaca atggatatac tctcccgttt cattgctttc
     3361 aacactcatt ccttcaaaga aaacactgcg acgatcatga ttcccacgac aaaaatagta
     3421 aggtgtggtt gggaaatatt gatccaacat tttccgtaag acttgataat cagaaatccc
     3481 cccttcatgt actaaatcac ctgttaatag aataaaatcg tacactgtta acgagagttc
     3541 ttgtaacaat ttttccaatg ccaactgtgg atttttaatt gttggaaata gttcagcagt
     3601 aaacaagtta gtttgatagt tttctctaaa atggatatca gagagatgca aaaagcgcat
     3661 ggctattccc tctttcttca ataaaataat caggatatcc taaattttct tgcgtctgat
     3721 aatcaaaaaa atgcattctt tcaggttcta tgcggagatt gactgcatca ccaatctgcc
     3781 aagtaggttt ttcattaagg acaatcattt ctataccatc gactttaacg gtgtacgcat
     3841 aactttgacc taatagctca cgatacttca cttgaccgtg taaatcgctt gtttcttttg
     3901 aaaaggttac gtgttctggt cttatgccta aatacaatga gccactaacc tgaagaagtt
     3961 gtttttgcca agctggattc aatgttaaaa attgactggc tacctttaat gttccttgat
     4021 cataagtaac tggcataata ttcatgcctg gcgaaccaat aaaatttgca gtaaaagtat
     4081 ttttaggttt cgtataaaca tttttcggcg tatcaatcat ctgaattcta ccttcattca
     4141 ataaaacaat ccggtcagct aacgtcatcg cttcaatttg atcatgagtg acgtagacaa
     4201 aagtttgttt ataacgttga tgaattttta ccaattcttt tcgtgcatcc aatcgtaaac
     4261 gcacatccaa atttgataac ggttcatcta ataaaaaata attactccgt ttaaccaccg
     4321 cacgagccaa tgctacacgc tgccgttgcc ctcctgacaa atctttagga agtcgatctt
     4381 ggtatggcgt taaacctaac atcttcaaag cttcttccaa acggtgctga atgtcagctg
     4441 tcttaatttt attcgccctt aatgcaaaaa taatattctc ctttacagtc atgtgaggat
     4501 ataaagcata attttgaaaa accattgcga tctctcgttc tccacaaggt agcttatttg
     4561 ctagttgtgt acctaaaaaa agctctccac tcgtaatttt ttccaaacca gcaatcattc
     4621 tcagcaaagt agattttcct gaaccagatg gtcccagtaa cacaattcgt tctccagcgg
     4681 ccacagctaa atcaatttct tttaaaatgg tatgttctcc atattttttt ccgatatttc
     4741 ttaattcaag cgtttctccc atgatttgtc ctccaattta tttaatacct gcagaaacaa
     4801 aagtcccgat aatatatttt tgaaaaattg catacaagac taacggtaca aacattgtaa
     4861 ttaaactaat cgccattgct acggtaaaat ttgtcccacc ttcagaacta atataggtct
     4921 gcatcgccaa cggcaaagta taattatcta ctgtccgtaa aattaatacc ggccaaacat
     4981 actcattcca agaggtaata aaaaaccaaa taccggtaga agtaatcgca tgtctagtaa
     5041 gaggaaaaac aattttactc atgacggttg tttgtttgat gttatccaat gccgccacat
     5101 ctagaaggct cactggaatt tcccgcattg tttgcgtcaa taaaaaaatg cccatcgcat
     5161 cagcaaattg tggtattatc acaccccaag tagtatcaat taaattaagt tttgcaatca
     5221 acaaataatt aggaatcatc gcaacagtaa aaggaatgaa catcgtacca atgatgataa
     5281 agtaccagat tttcttccct ttgattcggc aataagtaaa actatatgcc gcaaaaaaag
     5341 ctacgcctaa cttaacaacc gttataattg tagcaataaa aaaagtattt ctcgtaattt
     5401 tgataattgg aagattttcg aacaaatagc aaaaattttc caaggtaaac ggctgtggta
     5461 tcaaactagt aatcgtctga taggcatctt tcaatggctt aaaagaattg gccataataa
     5521 acaataatgg atagataata atgattatga tgaacataaa taagagatgt ggcaaccagc
     5581 gtttaatttt catagtaaac ccccttttcc acaaatttaa atattacccc taagaaaaca
     5641 gcaaatatta ataaagttaa aactgaaatt gctgaagatt ctccgacttt atacatttca
     5701 aaagcctttt gataagcata atagatcacg tttgcactac cattattcgg tccaccacga
     5761 gtgatgatct taatcggagt aaacacatac tgcagacctt gaacaatggt catcaccaat
     5821 aaatatagtc ctgtcgaact actcataggc aacacaattg taaaaaaaat gcgccagatg
     5881 ggtacacgat ccaacttagc tgcttcgatc atctcttgat caattcccac aattgctgca
     5941 aaaatgacaa tgaaattata accaaaaact ttccaagcag taatacctgc aatcacagca
     6001 attacccaac cttgtaaatt catccacata ggcagtctta aaccaaaatt tcccaacaag
     6061 acagcaattg gtcccgatac cggatttaat atccatgtaa aaaccatccc cgccactacc
     6121 aaagaaatca cagctggaat aaaaaataat ggtttataca aattctgccc tttttctata
     6181 actattccta ataaaaagga tgtaatatat gggaacacac agctcagcaa gacaaataga
     6241 acggtatagg acaaagtatt cttcaacact ttatgaaaga ctggatcagt cagcaccttt
     6301 acatagttgg caaagccaac aaattcttta gtgggggcca ccatattcca gtcaaaaaaa
     6361 ctcaaataga tcgtataaat caatggataa aaaacaaaca cgcccaacaa aataattgct
     6421 ggcgccagat acaaataacc tacatagcgc ttcatttccg acagcctcct catccttatg
     6481 tcaaaagcag acgaccttgt ttttcctttc ttacaggcgc tgtcgtctgc ttgctttgct
     6541 tatttgagta actcattaat ttccttttga gtagctttca tcgtttcatt gacggaatta
     6601 cctgataaaa ttttatcccg catatccaac aatttttgtt cagcttctaa tccagcatct
     6661 ccaggaaatg aactccaact gaccatctta tccatctgat caattgctgg tatcatcatc
     6721 tgattttcag ctaagaaagc ttttaatcca ttttgggcgt ctgcaacatc tttacgtggt
     6781 ggtaaataac cagtaccttc tgtccattta gctaaagatt caacactata aagatatttt
     6841 aagaaatccc aagttgcttc ctttttctta tccgtttgtg atgtaacagc taacattgcg
     6901 ccaccagcag gtagtttaac ttttttccct tcccaacttg gtgactcaac tgctttagca
     6961 gtaaaagtac tatttttttg aatgttgtcc ctttgagcaa tagtggtata gaccatcgcg
     7021 acgttaccat caataaagct ttgcacacct tgttcccacg tagtgtgtaa tgccgtctgg
     7081 tcttttacca tcctttgata taattcataa gcagcgatac catctttaga agcaaaggta
     7141 gctttgccat cttttattac acttgcccca ttactttcta aaattgcttg ttgtgcccaa
     7201 ttatcagcag gttcttgaat atacacgcca tactttccag ttttttcttt tattatctta
     7261 gaaaaagttt gaacttcttc ccaagttttt ggaccatttt catccaagcc cgctgctttt
     7321 aatatatctt gatttaaata taaaaccgga ttactaatgg agtaaggaat acccacttgt
     7381 ttaccatctt tttcagacag tgccatgata ttgtctaaaa aatgatcttt aaaataatca
     7441 gcattatccg gatccacttc ttctactaaa gattgaggat tagtatattt gaaattttct
     7501 gcaaaataat ctttaaaagc ccatcccact tgtactacgt ccggtatttc tttggaggca
     7561 acagaagctt gtaaattttg catcaagcct ttatacatat caggattata ttttgctaca
     7621 accttcactt tgtcttgaga ttgattgtat ttatctacta attcattcac tgtttttcca
     7681 cctagggtat ccgcattaac atgccaatac tctatcgtta cacgaccctc cgattttttt
     7741 gttgtcttgg tattcccact accacaagct gccaaagaga tgacacttaa aacagacaca
     7801 acccctataa caagattttt ctttaacata acattaactt cctcctaatt tctttatggc
     7861 catttttcta atcaagaaat attatgaact accaatgtaa atttctagtt aattttctgt
     7921 aaataattcg aaatttgtgc atttcggggt acagggtcac tggacatatc catcaagcta
     7981 agaataaagc ccccatatgg tcatgtatcc catctccaaa tctcttaact cctctgtttt
     8041 gattataaaa tagtttattc tgtgctcctt tcttccataa ataggcccat gcacaacgtg
     8101 aggttatatt ataaacacca cagcgcgcaa ggtactcaat cgtttctagt tcattgactg
     8161 tccatacaga aaatgtcaat cctagctgct gccccacttc acgggccaac ttagttaacc
     8221 atccttcatg aatattgacg atttgaacgc tttgataggc taatttctgt aaggtatcga
     8281 tcaaatctgc ttcggtaata tccccaagtt ttttcccttc gaactgattt tcaatattat
     8341 aaaatatatg ctttccccaa atcgcagccc aagataattg tacatagcca ctaagaacaa
     8401 tttgtggcca gatttgatag gtttgtgcca gcgaaaaaac ggccatttcc aatccctctt
     8461 cttttaaatc acagttaatg gtcatggttg ccttctcatt ggccactgct tgaaacacct
     8521 gctcaagggt taccgaatct gggacaactt cttacctgtc atgggctagt attagcgtac
     8581 catctgtttg ctgacgcaca tctacttcaa aacaaagctg gcgatccaaa caaaagcgaa
     8641 gaaatgccca gctattctcc tccgtattgt cactaccaga gtgtgcagtg atcatggtca
     8701 ttttgacccc tcctttaatt ccgtgatttt ctcccacacg tccactatac cgatgaagcg
     8761 ttaatttctt gttaatttct tgtaaattcc ctgcgttttt tcagtaaact agttgtaaat
     8821 cttgcatgct ccttcctacg aactcatgaa tcgtcgattt tgcccttcta gctagtttca
     8881 ccaattaagt agttctacat tccccgcttt taacctacta cacctttttt tgttttcagc
     8941 tattccttct tcttgctgaa agtccttgag gttctttctt acaggatacc atcaaggtgt
     9001 tcaatggctt attcccattg aatgaccgag acttactatc agatttctgc tcgttgaacc
     9061 aagtctttga tacaggattt cttccgtcat cttctcacaa ataccacctt gcgtttctct
     9121 agcgattggt cgatgtgcac cctcccagtg gactgtcact accccttttt gctatgccta
     9181 gcccacaaaa aaagctgtcc tcacagacag ccagactgta gacaaaagct gttccaaact
     9241 cgtaactcga gcttggaaca gctttttatc tacttcgttt cgaatttttg aacaaaaaaa
     9301 taaaggctcc ttgcctttcc atagccaggt ggctaatttc tttaaattca ttgcagcaaa
     9361 cactagcatc gtatgcgtcg cgactttttc caagcctcga tacttggtcc aacgcatccc
     9421 atgcttttct tttgcatccg caaatattcg ttcaatcgtt tgtttccgtt ttttgtaaat
     9481 tgatttattc agatctgtta ggcgaagatg ttccacttca tccatcagat ctccccaaac
     9541 atgtctcgtg atgactcgtt gatgattttt acttatggta cattggttta gcagaggaca
     9601 gttcacacat tcttttggat tggatttgta ttctcggtac ccttctcgag taaccgttga
     9661 aaaagcgagc atttgatctt gcggacaaat ataacaatca aaatactcgt catagagaaa
     9721 ctcatttttt gataatagcc cctttttccc tttgggcctt gaataaggca aacaaggtcg
     9781 aaggttcaaa tgagtcaaaa agtgaacaaa cttaggtgtc ttatacccag catcagcgac
     9841 aacacaattg atttcaggaa aaagtctctt cgattgtttg acgagct
//
LOCUS       DAZUKC010000089         9790 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000089 DAZUKC010000000
VERSION     DAZUKC010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.091"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(126..611)
                     /locus_tag="ACG80T_002278"
     CDS             complement(126..611)
                     /locus_tag="ACG80T_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HFS4841902.1"
                     /translation="MVRRVFFSFHYADVTRAMVVRNSWTLRGTKEAGFVDKAEFEKVK
                     RNGDIAIKNWIDNQLIGTSVTVVLIGKETLDREYVKYEIQESYKRGNAILAVNIGKIK
                     DFNQMTTTSQSVIKIIGQKPDGEYLWFDEIIDGQYDYISDDGYNNLGTWIDSAAYKKG
                     K"
     gene            complement(613..2049)
                     /locus_tag="ACG80T_002279"
     CDS             complement(613..2049)
                     /locus_tag="ACG80T_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HFS4841903.1"
                     /translation="MDKKVLIKRFSEAIEKGNAAIFAGAGLSMSQGYVSWPELLNDPA
                     TEIGLDSKKETDLVTLAQYYKNENGGSRGILNQILMDNFGEELEISENHRILASLPIE
                     TYWTTNYDHLIEKSIREAYKNPQVKKNYTQLATTNPNVDTIVYKMHGDIDDVSSTVIT
                     RDDYEKYDDDSYALFKETLKGDLLTKTFLFLGFSFTDPNLERILSDIRWVLRENQRPH
                     YCIMRKILKENFVDSEDFFDQERYNYELTKRRLQINDLSRFSINVVEVDDYSEITDIL
                     KSIRKKYLRKTIFISGSAVDYTPFTSESKGLKFVEKLAYRLSESGYRIVSGYGLGIGN
                     SIVSGVLKQRRNLRKNNIQDVLSLRPLPLDMPHEWRRYRENIIAESGISIFVFGNKLE
                     SGEIVTADGMIEEFELSVQNENIVVPIGFTKGASKVLFDKIQENFSDYFDDSLKSKFQ
                     ALEKLDTEGEVDKKVEQIVSLINSIQED"
     gene            complement(2082..2933)
                     /locus_tag="ACG80T_002280"
     CDS             complement(2082..2933)
                     /locus_tag="ACG80T_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HFS4841904.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTKGSNTLDILYK
                     NTIFTNL"
     gene            complement(3089..3334)
                     /locus_tag="ACG80T_002281"
     CDS             complement(3089..3334)
                     /locus_tag="ACG80T_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4841905.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            3439..4212
                     /locus_tag="ACG80T_002282"
     CDS             3439..4212
                     /locus_tag="ACG80T_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841906.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKIKSYDYPLNLTLNKGILSFVLAGYVDYEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTWLVDQLSTINTGFHELPKPDNETALVIKTF"
     gene            complement(4265..4579)
                     /locus_tag="ACG80T_002283"
     CDS             complement(4265..4579)
                     /locus_tag="ACG80T_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841907.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
     gene            complement(6098..6316)
                     /locus_tag="ACG80T_002284"
     CDS             complement(6098..6316)
                     /locus_tag="ACG80T_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841908.1"
                     /translation="MKLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKHEIKNNLKYCYLEKSEEIIHELNGF"
     gene            complement(6331..8958)
                     /locus_tag="ACG80T_002285"
     CDS             complement(6331..8958)
                     /locus_tag="ACG80T_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFS4841909.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTSNIVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVINTIVVGLMILSLMFVGYKMITGKGTIDLRSVGMNIVMSVALI
                     LLMPTMISSGIQFAKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENVQNSTSSQGFKSMDVRENVQNSTSSQGFKSMDVRENVQSSTSSQGFKTVDVRENV
                     QKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNE
                     LFRDTLNDPNPLFDNLLKK"
     gene            complement(8945..9163)
                     /locus_tag="ACG80T_002286"
     CDS             complement(8945..9163)
                     /locus_tag="ACG80T_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841910.1"
                     /translation="MIKNRLIDQMWCLDQNTDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQKMAQQLSESLRKWAEGENYESN"
     gene            complement(9160..>9790)
                     /locus_tag="ACG80T_002287"
     CDS             complement(9160..>9790)
                     /locus_tag="ACG80T_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841911.1"
                     /translation="VTLSNVMYIVLNVHKLIEITFPTLDCEKKEYMPKIRQRGIEKKR
                     YVSLKKGRGMYKFVYTYTKTTSQNISKTTLVMEEEDINIKRIYSKISVLYDANNEDGR
                     KLFQVYHYLRNANVDTEDIRKIIEYLSENTQYLIAECIIKQHEACFFQRKTEEGLYDY
                     VTYFLNGLQKRYKSQFLENVEPIDQFKKAFEIKELPEITMHNWLKGEES"
BASE COUNT         2982 a         1819 c         1249 g         3740 t
ORIGIN      
        1 gaattatgtc tgttcctaat tcagtttgat gaattaactg ataaaaaaat atctactatt
       61 taaagataca gggataagta agtaaaaaaa taacatgtca ttagatagta gacagttcca
      121 actctttatt tacctttttt gtatgctgca ctgtcaatcc aagttcctaa attgttgtat
      181 ccatcatcac ttatataatc atattggcca tcaataattt cgtcaaacca caaatattct
      241 ccatctggtt tttgcccaat tattttaatt actgattgac tagtagttgt catctggtta
      301 aaatctttta ttttacctat attaacggcc aaaatagcat ttcctcgctt ataactctcc
      361 tggatttcat atttaacata ttctcgatca agagtttctt tacctatcag caccacagtc
      421 actgatgttc caattaattg attatctatc cagtttttta tagcaatatc tccattacgt
      481 ttaactttct caaattcagc cttatctaca aaaccagctt cctttgttcc tcgcaaagtc
      541 cagctgtttc taactaccat tgctcttgtt acatctgcat aatggaaact aaaaaaaacc
      601 cttctcacca tattaatcct cctgaatact atttattaaa ctgacaattt gttcaacttt
      661 tttatctact tctccttcag tatctaattt ttctagcgct tgaaatttac tttttaagga
      721 atcatcaaaa taatcactga aattctcttg aatcttatca aacaaaacct tacttgcacc
      781 tttggtaaat ccaatcggaa ctactatatt ttcattttga acagataatt caaattcttc
      841 aatcattcca tctgctgtaa ctatttcacc tgattcaagt ttgtttccaa aaacaaaaat
      901 actaatccca gactctgcaa taatgttttc tcgatatctt ctccactcat gaggcatatc
      961 aaggggaagt ggtcttaaag aaagtacgtc ttgtatgtta ttttttctta aatttcttct
     1021 ctgctttaga actcctgaaa caatactatt accaattcca agtccgtaac ctgatactat
     1081 tctataaccg gattcagaaa gcctatatgc taatttctcg acaaatttta atcctttcga
     1141 ttcagatgta aatggagtat aatcaacagc acttcccgaa ataaaaatag tttttctcaa
     1201 atactttttt ctaatacttt tcaaaatatc agtaatctca gaataatcat ctacttcaac
     1261 aacgtttatc gaaaatctgg acaaatcatt gatttgtagt cttctttttg tcagttcata
     1321 attatatctc tcttgatcaa aaaaatcctc agaatcgaca aaattttcct ttaatatttt
     1381 tctcattata caataatgag gtctttggtt ttctctcaaa acccatctta tatctgataa
     1441 aattctttct aaattagggt ctgtaaaact aaatcctaaa aagagaaaag tttttgttaa
     1501 caaatctcct tttaaagttt ctttaaataa cgcgtacgaa tcatcatcat atttttcata
     1561 atcatctctt gttattactg tcgaactgac atcatctata tcaccatgca ttttataaac
     1621 aattgtatcc acatttggat tagttgttgc taactgagta taatttttct taacctgagg
     1681 gtttttataa gcttctcgga ttgatttttc tattagatga tcataattag ttgtccaata
     1741 agtttcaata ggtaaacttg ctagtattcg atgattttca gatatttcta attcttcacc
     1801 aaaattatcc ataagtatct gattcaaaat tcctcttgat ccaccatttt catttttata
     1861 atattgagcc agagtaacta agtctgtttc ttttttagaa tccaacccga tttctgttgc
     1921 aggatcatta agtagttccg gccaacttac atatccttgt gacatcgata atcctgcacc
     1981 tgcaaatatt gctgcatttc ctttttcaat agcttcagag aacctcttta ttaaaacttt
     2041 cttatccatt ctacccggtt cctttcgatt cagtaaattg attagagatt agtgaaaatg
     2101 gtgtttttat ataaaatatc taatgtatta cttcctttag tgcattccac agcatctaaa
     2161 gcccattttt tcaaatcagc ttttcgtaaa taagttaatt cacttaaatt taaagaatat
     2221 tttatttgca gattttcttg cgaagaatag tgctttataa tgcttctaac atcctcggtg
     2281 ctcatttgag gattactttc caatcgtgga ttcttttttt ccactattga agctgtcgtc
     2341 agttcgctat atagccatgg cgaatacgta aatttctgtt cattagtgat ttcattgctc
     2401 aaactaattg aattaggtgt attaagaaaa aacagacatt ctgtactatc aatcatatta
     2461 tttaaagctg tagaaagcat catatgaaca tgactagttg ttacatttct tttatcataa
     2521 tcatatgttt ttgtttgttc cttatagcaa tatttttggt ctattttttt taataaatca
     2581 gaactgtatc cccaaaccga agaatcaata aatgcagtaa gaccaaaatt ttcaaatagc
     2641 catgatgcaa aacgtatagc taatttttca tcagcatgag agtgagatat aaaaatatct
     2701 gcttttatag ttggaaacca atctttaata atttcgtctg catctagaat tttgctgtcc
     2761 ttagaataat aatcaactaa atcattttca ctttcaaata gattttctat tctcttgtta
     2821 atagattttt ggttgtgatc tatttgattt actacatgtg tatatgattg cgagcttgag
     2881 gagaatgcat ttaagtcagc atttgtaaaa tccaattcat atgccttata caaggatatc
     2941 cctccattct tgtattttga tccacaattt attaggcaac ataacagtat accctatcgt
     3001 tacatatccc attcattatt ataccagtat ttcttcgtga cacataaggg tgtaacataa
     3061 taatttcaaa cgaaatgact acaagtagtc attgaagcaa atcttcataa gacacctgta
     3121 gaatatcttt gatggctttc aattgatcca acttaatgtg ttggcggcca ccttcaatct
     3181 taaccaacgt ttccctagtt atagcaattt tacgaagctg taggtctcta atcagctcgg
     3241 tttgaccaat tcctttttct aatcggactc gtctaatatt gctaccgatt acattttctt
     3301 tatcaataat tttttcctct ttcaagaata gcacaccttt tggactaaaa ttagtccttt
     3361 ttcttattaa tcttaaagat tcggtatgct acaatgggac tagtattagt ccatattcaa
     3421 ttttaaggag gaattttttt gaagaaattt gcaatcattc taacttgcgc tttactcatc
     3481 ggaactataa gcgggtgtac cacttctgac acaagttcta caagtacgga aactagtaca
     3541 acaacctcag aaaagaatta cccaatacat ttttttccgg ttccagatgg atttgaagct
     3601 tattatcatg ataaaaaact agatgttcct ttatcagaaa ctgaatctac tgaaaagatt
     3661 ttgccaattg aaattgaaag aaatcaagac gatataaaac taaaagctga tcttccctta
     3721 ctgaacagca cacaaattga tagacatgaa aatattaaag ttaatattga aaaaggagcg
     3781 ttagcacaac tttatactga aaaagttgaa caattttttg aggctggtgc caataaagac
     3841 ttttcaaaaa taagcgatta ttcggataac ttttttaatg agacccaaga tgaaatcacg
     3901 aactcaactt ttatggctga agatatgtac aatatgaagt tgaaaaaaat aaagtcgtat
     3961 gattatccat taaatctaac attaaataaa gggatactct cttttgtatt agcaggttac
     4021 gttgactatg aatatacagc agattatttg gagacgcccc ttcaacacga taataacgat
     4081 attaatttta gatttatcta caattcaaaa agtaaaacat ggttagttga tcaattgagc
     4141 actataaata cgggatttca tgaattacct aagcctgaca atgaaactgc tttggtcatt
     4201 aaaacattct aaaaaagaaa cgttgaagga ataattattc tttcaacgtt tcttttattg
     4261 tttttcagtt tacggtctta cctgagcgaa taatctgatt tctaaatgat tggaaaactt
     4321 cttgtggttg ataataatct gtatctattt caaatgtttc tgagaataat tcattgaatt
     4381 tttctactga aatgatgatt ttaccattct cctctcgact gttttctatt atttctgcct
     4441 ctaattcaaa taaatctagt gacgaactaa aatcataaat cctgtcatat tgaacaaaaa
     4501 aatcaagaaa aagttgctca aactcttcct tatttatcac tagtgtttca agcggaacaa
     4561 ctttattttc atcagccatt cttccaattt cctcactttc attttcaatt gtccattgtt
     4621 gcggacgtat cagattttct ctatcatttt tcagtgtata gatcacttgt aatacacaac
     4681 aatgctttgt aaacttattt ttttagttag tcgaatcttt ttaaatgtgc gtttcatacc
     4741 atgcgcttga tatattttct cctatttttt aaatcgttta tcacaacttt tatcgttttt
     4801 tcttctgaaa aaaatcttgt ttacatcgtt acatagtttc caaaaagata cattactagt
     4861 agaaacaaca agattaattt tacttcccac tgaaatggga agtatatttt aaatattttt
     4921 tctccttcaa cttacactat aaagtatctt aatcacacga tatgctagcc actaaattca
     4981 tcatatttga ttcctttttt aagataatgt agcatctttt tatccaataa cgcaattgtt
     5041 tttaaggata aatttcttaa gatggtacat ttcaaaatgg catttgacat atgaaacaaa
     5101 aaagaaagaa aagagcataa gggaaaactg atagcacctt cctacaggaa ggtggctaag
     5161 ttggctgtgc caactggttt tctttcaaaa tcatttcata atttttgata tcataaaaaa
     5221 atccattttg gacctatttt cggtcataat ttagtaccta cttttggtca tagtttcgtc
     5281 cgtagttttg gtcatagtga ctaaggcttt tctgttcaca aaattggttt aaatttgctt
     5341 tctttaacac ttcatttata tcatagcaat tataatgttt acacaacatc attcctaatt
     5401 ttaacggttg ttcctcttta tattttttaa gttctacatt tttttgcgtt taattacttt
     5461 tctcttttct ttatattccg ctccaacttt ctctttaata gtgataatag aattacatat
     5521 ttttgttaaa cttccttaaa aaaattggat ctcgattact caaatttgct tgcaggcttt
     5581 tctaattgct ttgttttttc ttcctaatta tttgatttta tttccatttt cttctagtga
     5641 aaatttatta tttttaataa tcgtacgcgt agaactgatt tcattttttg tatctgtttg
     5701 attatctaac gtttgaaatt caaagttttg attggcttta tcaatatctt tttatactac
     5761 tttatttgca agatatattt tcttgcttaa tttggtcata gtttcattcg tcacttcggt
     5821 gatatttttt taatttgcaa ataaaaagag ttaaaccgat atacattagg ttcaactctt
     5881 ttgcaggttt catgggtgta ctatcacttt taatctgata gatgtaaacc tctctttatc
     5941 tttttgttac ttcaattttc aacaatagct cctttatagg ttgtcaatat tgctctacgt
     6001 attctggctt tttaacccat tatttctcta aagtggaaga gaataatcgc ttaatcccca
     6061 ctaattcctt gtcgtaaatt catgtcaaat acaattttta aaagccattt aattcatgga
     6121 ttatctcttc acttttttct agataacaat atttcaagtt gttttttatc tcatgtttca
     6181 tctttaattc ttcttgaatc atcagttcaa tataattagt gaccgtgatt ttttcatttg
     6241 ttgcgttctt tactgattca cataattcgt caagttctaa gaaatcatgc gtactaatct
     6301 ttagaattaa tttcatgaca attccccctt ttattttttt aatagattat cgaaaagcgg
     6361 attaggatca ttaagtgtat ctctgaataa ctcgttgtct tcataggtaa atcttcgtct
     6421 tggttttgat cccttatcat tttcacggat ttcactatca ataatcactg tttcatgatt
     6481 tgaatcaaat tttttcgctg actttctgtc ttcaacgata ttgatttttt gtgttttttc
     6541 tttcatttct ccactatctt tttgaacgtt ctcccgaaca tctactgttt tgaatccttg
     6601 actacttgta gaactttgta cattctctct tacatccatt gatttaaacc cttgactact
     6661 tgtagaattt tgtacattct ctcttacatc cattgattta aacccttgac tacttgtaga
     6721 attttgtaca ttttctcgaa catcaattgt cttagtacct tgaccatttg aagagctttg
     6781 taaattctct cttacatcaa cattccgtcc gacatgacca ttcaatgaac cttgaatatt
     6841 ttctcggaca tctactgatc ggccaccttg actacttgta gaattttgta gattttcttt
     6901 gaccaatttt tctaatccgg tatttgagtt tttagcttct tggattcttt ctttggtcaa
     6961 cgcttctttt tcgtccgtat tagctcctgt acgcccttct acacgtttcc ttaacatttc
     7021 ctcacgattc attgcttcag gcgtgtattt agcttcttgt cgcttctgat taattaattc
     7081 ttcagcagac ttagcttgac cgtgcatagc atttttacgt tcttcaatat cttgttgaac
     7141 tttttgctga attgcactta caggattatt attcgattca gcattatttt tcattgcttc
     7201 acgcatacgt tcagaactag acataattct atcggcattt tcaaccttag caccatcttt
     7261 tctatccgaa agacgtgttg gcatggaatc agcatatttt ccagtttcat cttctttatt
     7321 tttagctaac ataggtttgc tatttttatt aatagcggac actgtaccat catctaaacc
     7381 ttctttgaac ttattcttgg tatcattagc catatcacgc atactcttaa atggttttat
     7441 tgctctttca ctagccattg tagctccatc tgaagctaaa cctgataatc cacgttcatg
     7501 ggcatatccc aaagctcttc cagttttgcc agcttttttt gatagagttt cgccgaaatt
     7561 cttttctggt tttcgatctt ctccttctcc actgtctgat ccatttccat ttccgcttcc
     7621 tttagcacga ttaaatccag ttgatccttt tctaaacagc attgcgccca taccaaaagc
     7681 agaagcaagt tgcccatagc cttctttgag tccaatatca acaccaaagt atctcatgac
     7741 tgttccactg ccttttatca gcacaaacac agcacagata taagcaattg tttttataaa
     7801 aatatttgtg gagatacctt gtccagcatt taaaaaattg ataaacatag caaacataga
     7861 tagtccaaac ccttgaaatc caactgttaa gtaacattgt aaaatatcgg ataaaacaac
     7921 ttttgaacgt tgtcctgttt caatatctgt cgcaaaaaca agaacaccta acacacgttt
     7981 aaatgcaagt tctaatattg ctgtaatgat cacaaacgcg ctgaacacat aggcgattgc
     8041 caacgctgtc aacccaatcg aaatccccca aagatttgct tgatatcgat aataacctga
     8101 tttcaagcta tcagaaaatg ttgataagaa gttatcagaa aactttgtgg caacaaattc
     8161 attattagaa ttttcaacta attcatagcg aagattatcc gcagctggat tttctttttc
     8221 caatttatca atcactttat ccgtcaacac ttgtgttaaa tctgtctgtt gaaaattttt
     8281 tcgtgttaac ttatttcgat cctccgtttt atcaatgctt gagtattgat ctgttttatt
     8341 aatatatgcc agatctgtta ctccttgttt tatcaatgac caagcgactc cgtcatctga
     8401 atttgtaatc gtagtagcat cattgtaaaa tattttggca aattgaatac cgctagaaat
     8461 cattgttggc attaaaagaa ttaacgctac agacatcact atattcattc caacagatct
     8521 taaatctatt gttccttttc cagtaatcat cttgtagcca acaaacatca atgaaaggat
     8581 cattaatcca acgacaattg tatttatgac tgattgataa acattattta gccctgtact
     8641 ttcaatgaaa ttgaacaaat ccaacacatc tggaatcata ttttgaatcg aatcagtcac
     8701 agtaaacatg ccttttacca cagcccatcc aagccaaatc ccgaactgac taaggaaatc
     8761 tccattgtat ttttttaggt aaggtctata aagagaatta ttcaaaaact ctgtttgact
     8821 ttttgtgtca tcaaaccaat tgaaaatgct agcttcaaca atattacttg taaaaacaac
     8881 gctaatcatt aaaacaagta cactgagtgc taaccagttt atttttctac taacgatttt
     8941 ttctttaatt gctttcatag ttttctcctt cagcccattt tcttaggctc tcagagagct
     9001 gctgagccat tttttgatta attatgatat gagttacaca attgtcattt tcagctctta
     9061 aatcgagaac tactgtgttg aagcagatac tctcgattaa ttcgagagta tctgtatttt
     9121 gatcaagaca ccacatttga tctattaagc gatttttaat catgattctt ctcctttcaa
     9181 ccaattatgc atagtaattt caggtaactc tttgatttcg aaagcttttt tgaactgatc
     9241 tataggctct acattttcta aaaattgact tttatatctt ttctgaagtc cattcaaaaa
     9301 atatgttaca taatcgtata acccttcttc agtttttctt tgaaaaaagc atgcttcatg
     9361 ttgttttata atacactcag caataagata ctgagtattt tcagataaat attcaatgat
     9421 ttttctgata tcttctgtat ctacattagc gtttctcaaa tagtgataca cttgaaataa
     9481 ttttcgacca tcttcgttat ttgcgtcata aagaacacta atcttactgt aaatcctctt
     9541 tatattaata tcctcttctt ccataacgag agtagtcttt gatatgttct gtgaagtagt
     9601 ctttgtatac gtatatacaa atttgtacat acctctacct ttttttaaag agacatatct
     9661 ctttttttcg atacccctct gacgaatttt cggcatatac tccttttttt cgcaatccaa
     9721 agtaggaaaa gtaatttcta tcaatttatg tacatttaaa acaatgtaca taacatttga
     9781 taatgtaact
//
LOCUS       DAZUKC010000090         9297 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000090 DAZUKC010000000
VERSION     DAZUKC010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.092"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(78..2198)
                     /locus_tag="ACG80T_002288"
     CDS             complement(78..2198)
                     /locus_tag="ACG80T_002288"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS4841912.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2428..3606
                     /locus_tag="ACG80T_002289"
     CDS             2428..3606
                     /locus_tag="ACG80T_002289"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFS4841913.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3622..4380
                     /gene="aroD"
                     /locus_tag="ACG80T_002290"
     CDS             3622..4380
                     /gene="aroD"
                     /locus_tag="ACG80T_002290"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFS4841914.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4403..4888
                     /gene="ybaK"
                     /locus_tag="ACG80T_002291"
     CDS             4403..4888
                     /gene="ybaK"
                     /locus_tag="ACG80T_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFS4841915.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4959..6212)
                     /locus_tag="ACG80T_002292"
     CDS             complement(4959..6212)
                     /locus_tag="ACG80T_002292"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS4841916.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6328..7677
                     /gene="gdhA"
                     /locus_tag="ACG80T_002293"
     CDS             6328..7677
                     /gene="gdhA"
                     /locus_tag="ACG80T_002293"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFS4841917.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7789..9135
                     /locus_tag="ACG80T_002294"
     CDS             7789..9135
                     /locus_tag="ACG80T_002294"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFS4841918.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
BASE COUNT         2995 a         1680 c         1925 g         2697 t
ORIGIN      
        1 tattctaaga tttctcttta ccaacatcat ttgacaaaga gcctaatttt gtcgctgctc
       61 cttttaaatt tattttatca ttcggaagaa cttgtggtcg ttccgtgaag ttcttggtac
      121 gtttgcgttt tataaagatc aacagttgat tgtcccttgt gttcactaaa gacactagtt
      181 gacttgtcac caagagattt ttcagcttct tttaatttct ctaagccatt cttgtagtca
      241 taatcactgg tatcaattgg gtctaatcca ctattcgtat agaaccgaag aagatcacca
      301 ttattgattt ggtcagaaac agctaactgt ttatcaactt tttctttcag tgaatcaatt
      361 tcattctgta cttcctcagt aggttcagta atagcttcac cagtttcgtt actatagatc
      421 gtaccaccgt agtaagtata ttttggtgta acaaaatcac catcacggaa agaaacaagc
      481 tgttcatgat ctttcgagaa tagatcttgt cctaactgaa tataattttt tgtatctaca
      541 cctagtaagt gaagtaatgt aggcaaggca tcaacttgtc cgccgtaagt gtgattgatt
      601 ccgccctgtg tttgtccagg gatatggatc atataaggga cacgctgcat ttgtgcattg
      661 tcgtagtttg tccaagtaga acttgttttt ccaactaatt cagctagatt tttatttctt
      721 gaattcgaga caccgtaatg gtcaccgtaa aggacgatga ctgaattatc atacaagccg
      781 ctttctttca aataattgaa gaattcttgt acggcggtat ctaaataatt ggctgtcgca
      841 aagtaaccgt tgatcgtttc atcatttgta ttggctaacg ggaatcctgc ttcgtcattg
      901 gtgaattttg aataaggata atggttagat acagcaatga attttgaata aaatggttgt
      961 tgcagacgtt ccaagtattg tacggattgt tggaaaaatg gtttatcatg caacccgtat
     1021 tggaacgaat tgtcatcatt aacatcataa taagaagaat caaagaaata atcatatcct
     1081 aaattcttgt atgtctcgtt tcgattccag aatgtaccag cgttcccatg gaataccgca
     1141 ctagtgtaac cttgtgtttg acttaaaata tcaggtgcag cttggaaagt attttttccg
     1201 cctaattgtg tgaacaagga gccttgattc aagccaaaca atgaattttc catcagcgtt
     1261 tccgcatcgc ttgtttttcc tgcttttact tgatggaaaa agttgtcaaa actgaacgtg
     1321 ctgttgctgt ggtaaagact attgatgaaa ggtgtaactt caagctggag accattctca
     1381 tctgtcaatt tataatcgat caggaattgc tggaagcttt ctaaatggat ataaatcaca
     1441 ttttttcctt tagcgagccc gaacaaatca tcatttggtg ctgcataatg ttcttttaca
     1501 taattttcta cttctttcat atcattggga cttgcttctg cccgcacttg gtttgtctgg
     1561 taagtttgaa caccgtcata aaccatgaaa gcattcaagc caagatattt gaccaaataa
     1621 tcacgagaga atgttcgagt cagcaattct ggacgatcag cttccgccat aaaaagattt
     1681 cctgagaaaa tcataactgc taatgtagag atcgcaaaag ccattcttgc tcggatcggg
     1741 cgagggtcca acttgatttt ttttgcaatc agcagaacag tcaagataat aaaatcgatc
     1801 caatagatca catcatatgg gcgaaacagt cgaatggcac tttctcccag accactcgct
     1861 actttcccag cacctaaaat cgtgttgatc gtaatgaagt ccgtgaattc tcgataataa
     1921 gtgacattcg aaaaaacaag caatgtcaaa agaaaataaa tgaccatcat cgtgatataa
     1981 gcaactctag ggcgtctgat ataaagagca attccaagta ataacaatgt agttgcaata
     2041 gggttgataa acaagataaa gtattgaatg ccactttcga ttccgagatg gaaatcaact
     2101 aaataggcaa aaatcgtttt caaccaaagt aaaacaacta gtaaagaaaa aaatccgagc
     2161 ctcttattca aaaaggcagg tgtttttata catttcaaga taatcttcct ttctgcactt
     2221 cttaatcaca gaagtgtcat gaaaccatgt tacacagctc acttattctc atttatttta
     2281 ctagtatgac aatatagtgt caattgaaac cagaagttta tgctgtttca ttaaggtttt
     2341 ctttaagctt catgaaaaaa tgagcagtaa gacaagtcct atgttttttg ttatacttgg
     2401 gaagagaaag ctaaagcagg tgaaggaatg aaagtaaaag taaaaaaaag agcaagcaat
     2461 cgattgaaaa aaggatatcc actgatccaa aaagaagacc ttgctcatcc ccaaaaagaa
     2521 accacgcaat gggtggaatt agttgattcg caaaaccaat ttatcgcaaa aggatatcta
     2581 ggaaagcaga ataagggaat cggttgggta ttgagccagc agaacatccc atttgatcaa
     2641 tctttttttg aagaattttt tgtgaagtca aaagaaaaaa gaactttctt tttttcagat
     2701 gattctacta ccgcttttcg tatattcaat ggagaaggag acgggatcgg cggtctgatt
     2761 gttgattatt atgcaggtta tgctgttttt tcttggtata acgaaacgtt atatgcacat
     2821 cgtgaattat tgattcaagc ttttcgtgaa atctacccgg aaatcgtagg aggctatgag
     2881 aaaattcgat ttgaatcagt aaatctgcct gagagccagt tcatttacgg aaaagaggca
     2941 ccggaaccct tgatcgtaaa agaaaacgga gtaaaatttg ccgtttattt aaatgaagga
     3001 ttaatgactg ggatcttttt agaccaaaaa gaagtacggg gatcattagc agaaggtttc
     3061 gcttctggga aaacagtttt gaacatgttt agttataccg gcgccttttc tgttgctgcc
     3121 gcaatgggag gggcaatcga aacaacaagt gtcgatctag caaaacgcag tttaaaaaaa
     3181 acacaggaac agtttgaagt gaatggtttg gatgtcgatc agcaaaaaat cattgttatg
     3241 gacgtattcg agtatttcaa atacgccaaa agaaaacaat ttttatatga catgattgta
     3301 ttggatccac caagttttgc cagaaataag aaaaaagtct ttcgcgtagc aaaagattat
     3361 ggggaactgg tcaaggatag tctggatatt ttagcagaag acgggatact tattgcgtct
     3421 acaaatgctg cgaatgtctc gatagaacaa ttccaaacaa tgattgaaga ggaattccat
     3481 gccaaaaatg tttcttttga acaacttgct ttatatcgtc tgccaaaaga tttccagacg
     3541 acggatacct ttttagaagg caattatctg aaagtgtttg tttatcaaat taaaaataac
     3601 cattagaagg agcgatgcag aatggtttgg gtaaagggat tgaatatagg aagcggtaga
     3661 ccaaagattt gtgcgcctct tatcggggcc gtccgagagg aagtattacg tgaagcagtt
     3721 cttgcacgtg aagcaggggc cgatttagtc gaatggcgag tcgatcatta tcgtgaagtt
     3781 ttttcttttg atgaagtagc agaaacattg gttcttatcc atgagatgct tgatggactg
     3841 ccgttattat ttacgtttcg taccttagct gaaggcggag aacaagacat atcgatccgt
     3901 cagtatcgcg acctttatga acatgtgata cacacaggat tagtagatat ggtcgatatc
     3961 gagttgttca aagtagaaag tctaggaaaa agactgatag aggaaatcaa gtctttaaac
     4021 attcctttaa ttatcagtag tcatgatttt aaagaaacac cagcagaccc tgtcttgctt
     4081 taccgattga atgttatgga acattttgga gccgatatcg gaaaactggc agtaactccc
     4141 aataatgaac gagatgtttt gcggttgatg gagttgacta gacgggcaaa tgcttttgtc
     4201 tccatgccta ttattacgat gtccatggga aatcttggta aaatttctcg tctggctggc
     4261 ggaacgacgg gttccgttat gacatttggt tccttaagca gcgattctac ttctgctcca
     4321 ggacaattac cggtcaaagc attgaagcaa attattcaat tattaaatga accgttgtag
     4381 aacaagtttg aggtgatatt ttttggcaaa gaaaaaaata gtaaaaacga atgcaatccg
     4441 tatggtcgaa caaaaaaaga ttccctatac agagcacgaa tatgagtggg acgaaagcca
     4501 cctaagtgcg tcatcggtcg ctgaacagct tccagaaagc caatctcata tatttaaaac
     4561 actggttgct gtgggcaatg tgacggggcc tcttgttgct gtgataccgg gagaagccga
     4621 attgaatcta aaaaaattag caaaagtttc agggaataaa aaagtagaaa tgctccattt
     4681 aaaagatttg gaagcaacga ccggatacat acgtggtggc tgctcgccta taggtatgaa
     4741 aaaactattt cctacttatt tagatcaaat tgcagaaagc tatgaacaaa tcattgtctc
     4801 ggctggcaga agagggctac agatggagtt agcacctcaa gatatctgtg cattaacgag
     4861 cggacaattt gcagatataa aacaataaat catacgtata aaagaaagaa ggaggccggg
     4921 atgttgtttg tccagtctcc ttctttcttt tttatacatt atgctctttg cccggcattc
     4981 attcctgcta catgccctgt agcgaaagcc gctgtgatat taaagccgcc agtataacca
     5041 ttgacatcca gtaattctcc cgcaaaatac agaccatcaa tgattttact ctccaatgtt
     5101 ttaggataaa tttcctttag agaaacccct ccgccagtta caaatgattt ctcaatagga
     5161 aaagttttgt tgattgatag cttaaactct ttgcatagtt gaacaaaatc ttgaatttgt
     5221 ttttgagagg cttgttgtcc tgtcagatga tccatttcaa gccgctcaag aaagaaaact
     5281 aacaatcgct caggcaatat accatgccaa gcattgacca agttcttttt ggttgcttta
     5341 cttttttctg tcaatatatg aataagctct tgtttggttt gtgtagggaa acaatccaga
     5401 gaaagtgtca ctggttctcc cgttttctct aattctttat tgacaaaact tgaacaacgc
     5461 agtgcagccg gaccagaaat accgaaatgg gtgaaaagta aatccatcgt atgttcagtc
     5521 aatacacgac cttttttatt taaaacgcgt aaagtgatgt cttgtaaaga aagcccttga
     5581 agtgttttct cctgaatata cggttcatcc gatatcaagg gggactctgt cgcataaaga
     5641 ggcgtcaccg tgtgacccac tcgcttcacg atcttgtacc catctccagt agaaccagta
     5701 gatggataag tccgtccacc agttgttaag ataatggcag gagcatggaa ctcttcgaat
     5761 tccgtgcgta taccgtaaat tttatgttct ttgtggatca atttctctac tcgagtagag
     5821 aatagaagcg tcacatctag ttctttcaga cgattgagca atgcttcgat aatcgttttt
     5881 gatttatttg ttacaggaaa catccgtcca tgatcctctt cttttaaatg tacgccttgt
     5941 gattcaaaga agttcatgat atcgaaatta ttccattgcg caaacgtact atataaaaac
     6001 tttccatttc caggaatatg tgcaatcaaa tcatcaacag gacgattgtt cgtaacattg
     6061 caccgaccac cacctgtcat cagtagtttt tttcctgctt ttttgttttt ttcgatcaag
     6121 agtacgcgtg cgccttgttc agctgcactg atcgctgcca tcatcccgct tgttccagcg
     6181 ccgactacga tcacatcata cagtttttcc attccctcac ctcaaggaaa gtgtagcata
     6241 tctgaagaaa gaaaacagaa aaaatgtttt gagccaaaag agaaaatgag ctaaaatgag
     6301 ggaagataag caaaggagcg tggaacgatg acaaacgcaa cagaatatgt tcaagcaatt
     6361 caagaaaagc tacatcaaaa ggatcaaggg caagaagaat ttttacaagc aatcgatgaa
     6421 ttcatgccca cggttatgtc ttttttggac actcatcccg aatatatcga aaagaatatc
     6481 ttagaacttt taacggaacc agaaagagtt attcaatttc gagtgccttg gcaagatgat
     6541 gaaggaaact ggcgagtaaa tagagggtac cgtatccaat acaactcagc tatcggccct
     6601 tataaaggtg gattgcgctt ccatcctagt gtcaatctaa gtatcttgaa atttttggct
     6661 tttgaacaga ttttcaagaa tagtttaacc ggtctgccaa tcggtggagg aaaaggcgga
     6721 agtgacttcg atcctaaagg aaaatctgat aatgagatca tgcgtttttg tcaaagtttc
     6781 atgctggaat tgtcaaaaca tatcggccca tcgattgatg ttcctgcggg agatatcggt
     6841 gtaggagcta gagaaatcgg ctatttgttc ggcgaatata aacgattgaa aaaatatgat
     6901 actggtgtgt tgacaggtaa acctttggat ttttggggaa gtaagatacg tacggaagct
     6961 actggttatg gattagtgta ttatgtcaaa catctattga acgaagaaaa agattcattt
     7021 aatcaaaaaa cagtttttgt ttccggaagc gggaatgtag ccatttatgc aatcgaaaaa
     7081 gtgcaagagc taggcgggaa agttgttaca tgttcagatt ctagcgggta catctatgat
     7141 ccagaaggaa tagatgtgaa actattgaaa gaagtcaaag aaatcaatag acaacgtttg
     7201 acggaatacg caaaccaacg tccttctgct atttatcatg ctggagaatc tgtttggaca
     7261 ttgaaagaaa aagcggatat cgctttgcca tgtgctactc aaaatgaaat agacgaaagc
     7321 ttggccgaaa tcttagtaga aaatgggatc aaaatcgtag cagaaggcgc taatatgcca
     7381 tgtacagtaa aagctgtttc tgttcttcat gaagcaggcg tgtggtattg tccaggaaaa
     7441 gctgctaatg ctggaggagt agcagtatct gctttagaaa tgagtcagaa cgcccagcga
     7501 ttgacttggg aaagagagca agtggatgag cagctggatc aaatcatgga aacgatttac
     7561 catacgtgtc gccaaacagc agccgagtac ggcgacgaaa aaaatctgct tcttggagca
     7621 aatgttgctg gatttgaaaa agttgccaaa gcaatggcag cagaaggttt agtttgaaaa
     7681 atgaaacttt cttggaaata ttgtcagcat tttgtttatt tgcttgaaaa ataataaaaa
     7741 aaaacgtaaa gtattacctg tagagtaaac ctataaggag gagaacaaat ggcacacatt
     7801 cattttgact attcaaaagt agcaccgttt gtcaacgaac aagagttagg gtacatgcaa
     7861 agccaagtaa cagcagccca caacgaatta cgggaaggta ctggcgcagg aagcgacttc
     7921 cgcggctgga tcgatttacc aacgaactat gacaaagaag aatttgcacg tatcaaagaa
     7981 gcagctaaaa aaatccaatc tgattctgaa gtattagttg ttatcggtat cggtggttct
     8041 tatttaggcg ctcgtgcagc gatcgacttc ttgaatcaca cattcttcaa tttattagat
     8101 agcaacacaa gaaaagcacc acaaatcttc tttgccggaa attcaatcag ctcaacatat
     8161 attgctgact tgatcgaagt aatcggtgat cgcgactttt cagtaaacgt tatttcaaaa
     8221 tctggaacaa ctactgaacc agcgattgcg ttccgtgtat tcaaagagct tcttgttaag
     8281 aaatatggac aagaagaagc aaacaaacgt atctacgcta caacagataa agcaaaaggt
     8341 gcagttaaag tagaagcaga tgcagaaggt tgggaaactt tcgtgattcc tgatgacatc
     8401 ggcggacgtt tctctgtatt aactgctgtt ggtttgttac caatcgctgt tagtggtgca
     8461 gatatcgatg cattgatgca aggtgccgct gatgcaagca aagcatactc aagcgacaaa
     8521 ctagaagaaa acgaagctta ccaatacgca gcaatgcgca acattcttta ccgtaaaggc
     8581 aaagtaactg agttgttgat caactatgaa ccaggaatgc aatacttctc agaatggtgg
     8641 aaacaattgt atggtgaatc tgaaggaaaa gaccaaaaag gcatctatcc ttcaagcgca
     8701 aacttctcaa ctgatttgca ctctttagga caattcatcc aagaaggacg tcgcaatatt
     8761 tttgaaacag ttgtgaaggt tgaaaaacct cgtaaatcaa tcacaattcc tgaacaagca
     8821 gaagatttag acggtctagg ctacttgcaa ggaaaagaag tggacttcgt aaatacaaaa
     8881 gcatttgaag gaacattgct tgctcataca gacggtgacg tgcctaactt gctagtcaaa
     8941 attccagaaa tggatgctta tacattgggt tatacaatgt acttctttga aattgctgta
     9001 ggtatttcag gttacttgaa tggaatcaac ccattcgacc aaccaggtgt agaagcttac
     9061 aagaaaaaca tgttcgcact tctaggcaaa ccgggctttg aagatcttgc taaagaattg
     9121 aacgaacgcc tgtaagataa gggtttttcg ctagatttaa ataatcagtg atagaaacat
     9181 gttcgcttta aaatgaaaac tggtacccat ttaggtactc aaacatactg catcgtttat
     9241 tcagaataag gggctgtgac aagacgtttg tcacagtccc ttgtttgtat agaaatc
//
LOCUS       DAZUKC010000091         8935 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000091 DAZUKC010000000
VERSION     DAZUKC010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.093"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..333)
                     /locus_tag="ACG80T_002295"
     CDS             complement(13..333)
                     /locus_tag="ACG80T_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFS4841919.1"
                     /translation="MNSLSTSPLKNESIQEVSKIFKMISDPTRLSILFLLQKEELSVG
                     AIAHSLSMEQSAISHQLKTLKTSRLVKSKRAGKNMIYSLDDLHIFSILEQVSTHIEEQ
                     EKEK"
     gene            589..2496
                     /locus_tag="ACG80T_002296"
     CDS             589..2496
                     /locus_tag="ACG80T_002296"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS4841920.1"
                     /translation="MKEIQEQHSHENNSHDHGKMPIILYFIGLALSLIALFVNGENNL
                     IQNTLFSIASISAGYHVIILEGIGETIENSKRQHKFTPNSHILMGIAAIGASLIGNFW
                     EGTLLVLIFSGAHFLEDYAEGRSKREITKLLEMNPTTARLIMQDGNTTIVNVSELKVG
                     DQLQVLNGDQVPIDGVIVSGSTSIDESSINGESIPKEKSKGDGVFGSTINGTGTFTME
                     VTKETNDTVFSKILQLVNQNQENQTKASSIIQKFEPKYVKFVLIAIPLFILLAPMLFD
                     LTWSQSIYRGLVLLVAASPCALAAATVSVTLSATSNLAKRGVLSKGSAYLSQLADIQA
                     IAFDKTGTLTKGKPEVTNYYFSDSVNEENMIDIVVALEKESNHPLASAILDKFEPKNK
                     LAIEVENQIGKGLIGDYNENTYRIGKPTSFEAVSDDYTRLNKEWASEGNTVVYVSENE
                     KVIGLIALMDIPSEHAKATIDYFKKCGIHTTLITGDSEMTGQAVGKQLGVDQVIANVM
                     PEDKSNIINEQKETYGITAMVGDGINDAPALVKADVGIAMGEGTDVAVDVSDLVLMKN
                     DLSKLVKAHEISLKMNRVIWQNIIFSMVVVAFLITVSLVGLTDIAISVIVHEGSTLVV
                     ILNGLRLLKSK"
     gene            2590..2811
                     /gene="relB"
                     /locus_tag="ACG80T_002297"
     CDS             2590..2811
                     /gene="relB"
                     /locus_tag="ACG80T_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFS4841921.1"
                     /translation="MTVISLRIDTQEDKLIKEYAKAKNISVSALIRSSVLEKIEDEID
                     LGLYNQAMKDHKENPKSISFEDMLKELSD"
     gene            2812..3084
                     /locus_tag="ACG80T_002298"
     CDS             2812..3084
                     /locus_tag="ACG80T_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS4841922.1"
                     /translation="MSSGYQVEFEKGAQKALKKMDKYQSLLIMGWIQKNLVNCTDPRQ
                     HGKGLTVNRSGEWRYRIGDYRLIADINDETVTILMLEIGHRKNIYK"
     gene            complement(3215..6181)
                     /locus_tag="ACG80T_002299"
     CDS             complement(3215..6181)
                     /locus_tag="ACG80T_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFS4841923.1"
                     /translation="MKIARGRELLTPEQRQAFMQIPEDEWILGTYFTFSKRDLEIVNK
                     RRREENRLGFAVQLAVLRYPGWPYTHIKSIPDSVIQYISKQIGVSPSSLDHYPQRENT
                     LWDHLKEIRSEYDFVTFTLSEYRMTFKYLHQLALENGDAIHLLHECIDFLRKNKIILP
                     AITTLERMVWEARAMAEKKLFNTVSKSLTNEQKEKLEGIITSQHPSESNKTILGWLKE
                     PPGHPSPETFLKIIERLEYIRGMDLETVQISHLHRNRLLQLSRLGSRYEPYAFRDFQE
                     NKRYSILTIYLLQLTQELTDKAFEIHDRQILSLLSKGRKAQEEIQKQNGKKLNEKVIH
                     FTNIGQALIKAREEKLDVFKVLESVIEWNTFVSSVEEAQELARPADYDYLDLLQKRFY
                     SLRKYTPTLLRVLEFHSTKANEPLLQAVEIIRGMNESGKRKVPDDSPVDFISKRWKRH
                     LYEDDGTTINRHYYEMAVLTELREHVRAGDVSIVGSRQYRDFEEYLFSEDTWNQSKGN
                     TRLSVSLSFEDYITERTSSFNERLKWLAANSNKLDGVSLEKGKLSLARLEKDVPEEAK
                     KFSASLYQMLPRIKLTDLLMDVAHITGFHEQFTHASNNRKPDKEETIIIMAALLGMGM
                     NIGLSKMAEATPGLTYKQLANVSQWRMYEDAMNKAQAILVNFHHKLQLPFYWGDGTTS
                     SSDGMRMQLGVSSLHADANPHYGTGKGATIYRFTSDQFSSYYTKIIHTNSRDAIHVLD
                     GLLHHETDLNIEEHYTDTAGYTDQIFGLTHLLGFKFAPRIRDLSDSKLFTIDKASEYP
                     KLEAILRGQINTKVIKENYEDVLRLAHSIREGTVSASLIMGKLGSYSRQNSLATALRE
                     MGRIEKTIFILNYISDESLRRKIQRGLNKGEAMNGLARAIFFGKQGELRERTIQHQLQ
                     RASALNIIINAISIWNTLHLTTAVEYKKRTGSFNEDLLHHMSPLGWEHINLLGEYHFN
                     SEKVVSLNSLRPLKLS"
     gene            6327..6902
                     /locus_tag="ACG80T_002300"
     CDS             6327..6902
                     /locus_tag="ACG80T_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4841924.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            7116..7811
                     /gene="vanR-A"
                     /locus_tag="ACG80T_002301"
     CDS             7116..7811
                     /gene="vanR-A"
                     /locus_tag="ACG80T_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFS4841925.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            7789..>8935
                     /gene="vanS"
                     /locus_tag="ACG80T_002302"
     CDS             7789..>8935
                     /gene="vanS"
                     /locus_tag="ACG80T_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS"
                     /protein_id="HFS4841926.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKR"
BASE COUNT         2885 a         1542 c         1703 g         2805 t
ORIGIN      
        1 tgtagtttgt ctttattttt ctttttcttg ttcttcgata tgagttgaaa cttgctcaag
       61 aatactaaaa atatgaagat cgtcaaggct ataaatcata tttttaccag ctctttttga
      121 ttttactaat cttgaagtct ttaaagtttt taactggtga gaaattgctg attgttccat
      181 actcaaagaa tgcgcaatag ctccaacgct aagttcttct ttctgcagaa gaaataaaat
      241 tgaaagtcgg gtagggtcac tgatcatttt aaatatttta cttacctctt ggatagattc
      301 attttttaat ggagaagttg ataatgaatt cataagaatt tctctccttc atatgtttgt
      361 ttattcacat ttagattatc ataatatttt tataaagcaa gattttttgt ataagggtag
      421 ctattttaga gtcctatttt ttttatcacc taaataagat aactattttt ttgacaaatt
      481 agatagagcg atataaaatg aatgtataga catatgaatg ttcgttcaaa cgtaattcgt
      541 aagcattacg atttctaaaa aaaagtttta ttaagaaagg tgatagaaat gaaagagatt
      601 caagagcaac actcacacga aaataatagt cacgatcatg ggaaaatgcc aattattttg
      661 tactttattg gtttagcatt gtcgctcatt gcgttatttg taaacggaga aaataattta
      721 atccaaaaca ctttgttttc aattgcttca attagtgctg gttatcatgt cattattctt
      781 gaaggaattg gagaaacgat tgaaaactca aaaagacaac acaaatttac ccctaattct
      841 catattttaa tgggaatagc tgcaataggt gcttctctga taggaaattt ttgggaagga
      901 acgttattgg ttcttatttt ctctggagca cattttcttg aagattatgc tgaaggaaga
      961 agtaaaagag aaattaccaa gttattagaa atgaatccaa caacagctag gctaatcatg
     1021 caagatggca atacaacaat cgttaatgtt agtgagttga aagtaggaga tcaacttcaa
     1081 gtgttaaacg gtgaccaagt accgattgac ggagtaatag tatcaggctc tacttcgatt
     1141 gacgaatctt ctattaatgg tgagagtata cctaaagaga aatcaaaggg agacggagtt
     1201 tttgggagta caattaatgg aacgggtacc tttactatgg aagtcacaaa agaaactaac
     1261 gatactgtct tttcaaagat tttacaacta gtgaatcaaa atcaagagaa tcaaacaaaa
     1321 gcgtctagta ttatccaaaa gtttgagcct aagtatgtta aatttgtttt aattgcaatc
     1381 ccactcttca ttttacttgc tcctatgctg tttgatttga catggtcaca aagtatttat
     1441 agaggattag tccttttagt cgcagcttct ccgtgtgctt tagcagcagc taccgtatcc
     1501 gtaacattgt cagccacttc taatctagca aaaagaggcg tactttcaaa aggaagtgcc
     1561 tatttgtccc aattagcgga tattcaagcc attgcctttg ataaaacagg aacattaact
     1621 aaagggaaac cggaagtaac caattattac ttttctgatt ctgtgaacga ggaaaatatg
     1681 attgatatcg tagtggctct tgaaaaagaa tcaaatcacc cgttagcaag tgctatttta
     1741 gacaagtttg aaccaaaaaa taaactagct attgaagtgg aaaatcagat tggaaaaggt
     1801 ttaataggcg attataacga aaacacttat cgtataggta aaccaacttc ttttgaggct
     1861 gtatcggatg actatacacg tttgaataaa gaatgggctt cagaaggtaa tacggttgta
     1921 tatgtatcag aaaatgaaaa agtaatcggc ctgatagcat taatggatat tccaagtgaa
     1981 catgcgaaag caactataga ttactttaag aagtgtggta tccataccac tttaattact
     2041 ggggattctg aaatgacagg gcaagctgtt ggtaaacaat taggagtaga tcaagtaatt
     2101 gcaaacgtca tgccagaaga taaatctaac attatcaatg aacaaaaaga aacctatggt
     2161 ataaccgcta tggtgggaga tggtataaac gacgcacctg ctcttgtgaa ggcagatgtt
     2221 ggaattgcta tgggagaggg tacggatgtt gcagtagatg tatctgatct agtattaatg
     2281 aaaaatgatt tgtctaaatt ggtaaaagct catgaaattt ctttaaaaat gaatcgagtt
     2341 atttggcaaa atattatttt ctcaatggta gttgtcgctt ttctaattac cgtaagctta
     2401 gtaggattaa cggatatagc cattagtgtg attgttcatg aaggaagtac cttagtcgtg
     2461 atattaaatg gtcttcgatt attaaagtct aaataaatac atgaaattgg ataaaatcat
     2521 gtatctaaag tttgcagcct ctctaatttt gtattacaat gtatatacaa aaagaagggg
     2581 gtgattctaa tgactgtcat atcgctcaga attgatactc aggaagataa attaattaaa
     2641 gaatatgcta aggcgaagaa tatttcggtt tcggctctga ttagaagttc tgtcttggaa
     2701 aaaatcgagg atgaaattga tttgggccta tataatcaag ccatgaagga tcataaagag
     2761 aatccgaaaa gtatttcttt tgaagacatg ctaaaggagc tatctgatta aatgagctca
     2821 ggttatcaag tcgagtttga aaaaggagct caaaaagcgt taaaaaaaat ggacaaatac
     2881 caatcccttt taattatggg atggattcaa aagaatctag tgaattgtac cgatcctagg
     2941 cagcatggaa aaggattaac cgtcaatcga tcaggcgaat ggcgctatag aatcggtgat
     3001 tatcgcttaa tagctgatat caatgacgag accgtaacca ttttaatgtt agagattggt
     3061 catagaaaaa atatttacaa ataaaccatt caaattgagt gcaagcatgt ttaataaaaa
     3121 aggattctac tgctttttaa ggggtagcgt caggaaaatg cggatttaca acgctaagcc
     3181 tattttcctg acgaatccct cgtttttaac aacgttaaga aagttttagt ggtcttaaag
     3241 aatttaatga gactactttc tctgagttaa aatggtattc tcctagtaaa ttaatatgtt
     3301 cccaacctaa gggcgacata tggtgtaaca aatcttcatt aaagctacct gtccgttttt
     3361 tatattcaac tgctgttgtt aggtggagag tattccaaat acttatagca ttgataatta
     3421 tgtttaaagc actggctctt tgcaattgat gctgtatggt gcgttctcta agctcacctt
     3481 gttttccgaa gaaaatagct cttgccaatc cattcatggc ttctccttta ttcaatcctc
     3541 tttgtatttt tcttcttaat gattcatccg atatataatt caaaataaag atcgtttttt
     3601 ctattcggcc catctcacgt aaggctgtag ctaagctgtt ttgtcttgaa taggaaccta
     3661 gcttccccat aataagggat gctgaaactg ttccctccct tatagaatga gctaatcgca
     3721 aaacatcctc ataattttct ttaatgacct ttgtatttat ttgtccacgt aaaatggctt
     3781 ctagttttgg atactcactt gctttatcta tcgtaaataa ttttgagtcc gataaatccc
     3841 ttattcttgg ggcaaattta aatcctaata aatgagtcag tccgaatatt tggtcagtgt
     3901 aaccggcagt gtctgtataa tgttcctcta tgtttagatc cgtctcatga tgtaacaaac
     3961 catccaaaac atgaatcgca tctcttgaat tagtatgaat aatctttgtg tagtaagaag
     4021 agaattgatc acttgtaaat cggtagatgg tggctccttt tccagttcca taatgtggat
     4081 ttgcatctgc atgtagtgat gaaacaccta gctgcattct cataccatct gacgaagatg
     4141 ttgtaccgtc gccccaatag aaaggcaatt gtaatttatg atgaaagttt actaatatgg
     4201 cttgggcttt attcatggca tcttcataca tgcgccattg agatacattg gctagttgct
     4261 tatatgtaag tccgggtgtg gcttcggcca tcttgctcaa gccaatattc attcccattc
     4321 ctaaaagggc agccatgata atgattgttt cttccttatc tggttttcga ttattggaag
     4381 catgagtgaa ttgctcatga aatcctgtta tatgggccac atccatgagt aaatcagtta
     4441 attttattct tggtagcatc tgataaaggc ttgcactaaa tttttttgct tcttctggaa
     4501 catctttttc taagcgtgca agtgatagct ttcctttttc aagagaaacc ccatctaact
     4561 tattggaatt ggcagctaac cactttaacc tttcattaaa gctgctggtt ctctccgtta
     4621 tataatcttc gaatgataaa ctaactgata atctcgtatt ccccttcgat tgattccatg
     4681 tatcttccga aaacaaatat tcctcaaaat ccctatattg tctgctgcca acaatggaaa
     4741 catctcctgc ccgaacatgc tcccgaagtt ctgttaaaac agccatttca tagtaatgac
     4801 gattaattgt tgtaccatca tcctcgtata aatgtctttt ccatcgtttt gaaataaaat
     4861 ccacaggtga gtcatcaggc acttttcgct ttccagattc gttcattcct cggataatct
     4921 caacagcttg taaaagtggc tcatttgcct ttgtagaatg aaattccaat actcttaata
     4981 gcgttggcgt atattttctt agtgaataaa accgtttttg cagtaagtct aaataatcat
     5041 agtcggcagg acgtgcaagt tcctgagcct cttctactga agagacaaag gtattccatt
     5101 caataaccga ttctaaaacc ttaaaaacgt ctaatttttc ctctcttgct ttaattaatg
     5161 cttgtccgat gttcgtaaag tgtataactt tctcatttag ctttttaccg ttttgtttct
     5221 ggatttcctc ttgagcctta cgaccttttg ataacaaact aagtatttgc ctatcatgaa
     5281 tttcaaacgc tttatccgtt agctcctgag taagttgtaa taaatagatg gttaatatcg
     5341 aataacgttt attttcttga aagtcacgga atgcatacgg ctcgtatctt gagcctaagc
     5401 gagacagctg caacaggcgg ttacggtgca aatgactaat ttgcactgtt tctaaatcca
     5461 ttcctcgtat gtattcgagt cgttctatta tttttagaaa agtttcgggt gaaggatgac
     5521 ccggtggctc ttttaaccaa cccaatatcg ttttattgga ttcggatgga tgctgcgagg
     5581 taataatccc ttcaagcttt tctttttgct catttgttag agatttacta accgtattaa
     5641 atagcttctt ttcagccatt gcccttgctt cccacaccat tctttcaagt gtagtgatag
     5701 caggcagtat aattttgttt tttcttagaa aatctatgca ttcatgcagt agatgaatgg
     5761 catcaccatt ttccaaagct aattgatgaa ggtacttaaa tgtcattcga tattcactca
     5821 gggtaaaagt tacaaagtcg tattcacttc gaatttcttt caaatgatcc caaagtgtat
     5881 tttccctttg aggataatga tcaagcgagg atggactaac accaatctgt ttcgatatat
     5941 attgtatgac cgaatctggg atgcttttga tatgagtgta tggccaaccg ggataccgaa
     6001 gaacagctaa ttgaacagca aatcctaaac ggttttcttc cctccttcgc ttattaacta
     6061 tttctaaatc ccgtttggaa aaagtgaagt aggtccccag tatccattca tcttcaggga
     6121 tttgcataaa agcctgtctc tgttccggtg taagcaattc tctacctctc gcaattttca
     6181 ttcagtatca ttccatttct gtattttcaa tttattagtt caattatata tcaatagagt
     6241 gtactctatt gatacaaatg tagtagactg ataaaatcat agttaagagc gtctcataag
     6301 acttgtctca aaaatgaggt gatattttgc ggaaaatcgg ttatattcgt gtcagttcga
     6361 ctaaccagaa tccttcaaga caatttcagc agttgaacga gatcggaatg gatattatat
     6421 atgaagagaa agtttcagga gcaacaaagg atcgcgagca acttcaaaaa gtgttagacg
     6481 atttacagga agatgacatc atttatgtta cagacttaac tcgaatcact cgtagtacac
     6541 aagatctatt tgaattaatc gataacatac gagataaaaa ggcaagttta aaatcactaa
     6601 aagatacatg gcttgattta tcagaagata atccatacag ccaattctta attactgtaa
     6661 tggctggtgt taaccaatta gagcgagatc ttattcggat gagacaacgt gaagggattg
     6721 aattggctaa gaaagaagga aagtttaaag gtcgattaaa gaagtatcat aaaaatcacg
     6781 caggaatgaa ttatgcggta aagctatata aagaaggaaa tatgactgta aatcaaattt
     6841 gtgaaattac taatgtatct agggcttcat tatacaggaa attatcagaa gtgaataatt
     6901 agccattctg tattccgcta atgggcaata tttttaaaga agaaaaggaa actataaaat
     6961 attaacagcc tcctagcgat gccgaaaagc cctttgataa aaaaagaatc atcatcttaa
     7021 gaaattctta gtcatttatt atgtaaatgc ttataaattc ggccctataa tctgataaat
     7081 tattaagggc aaacttatgt gaaagggtga taactatgag cgataaaata cttattgtgg
     7141 atgatgaaca tgaaattgcc gatttggttg aattatactt aaaaaacgag aattatacgg
     7201 ttttcaaata ctataccgcc aaagaagcat tggaatgtat agacaagtct gagattgacc
     7261 ttgccatatt ggacatcatg cttcccggca caagcggcct tactatctgt caaaaaataa
     7321 gggacaagca cacctatccg attatcatgc tgaccgggaa agatacagag gtagataaaa
     7381 ttacagggtt aacaatcggc gcggatgatt atataacgaa gccctttcgc ccactggagt
     7441 taattgctcg ggtaaaggcc cagttgcgcc gatacaaaaa attcagtgga gtaaaggagc
     7501 agaacgaaaa tgttatcgtc cactccggcc ttgtcattaa tgttaacacc catgagtgtt
     7561 atctgaacga gaagcagtta tcccttactc ccaccgagtt ttcaatactg cgaatcctct
     7621 gtgaaaacaa ggggaatgtg gttagctccg agctgctatt tcatgagata tggggcgacg
     7681 aatatttcag caagagcaac aacaccatca ccgtgcatat ccggcatttg cgcgaaaaaa
     7741 tgaacgacac cattgataat ccgaaatata taaaaacggt atggggggtt ggttataaaa
     7801 ttgaaaaata aaaaaaacga ctattccaaa ctagaacgaa aactttacat gtatatcgtt
     7861 gcaattgttg tggtagcaat tgtattcgtg ttgtatattc gttcaatgat ccgagggaaa
     7921 cttggggatt ggatcttaag tattttggaa aacaaatatg acttaaatca cctggacgcg
     7981 atgaaattat atcaatattc catacggaac aatatagata tctttattta tgtggcgatt
     8041 gtcattagta ttcttattct atgtcgcgtc atgctttcaa aattcgcaaa atactttgac
     8101 gagataaata ccggcattga tgtacttatt cagaacgaag ataaacaaat tgagctttct
     8161 gcggaaatgg atgttatgga acaaaagctc aacacattaa aacggactct ggaaaagcga
     8221 gagcaggatg caaagctggc cgaacaaaga aaaaatgacg ttgttatgta cttggcgcac
     8281 gatattaaaa cgccccttac atccattatc ggttatttga gcctgcttga cgaggctcca
     8341 gacatgccgg tagatcaaaa ggcaaagtat gtgcatatca cgttggacaa agcgtatcga
     8401 ctcgaacagc taatcgacga gttttttgag attacacggt ataacctaca aacgataacg
     8461 ctaacaaaaa cgcacataga cctatactat atgctggtgc agatgaccga tgaattttat
     8521 cctcagcttt ccgcacatgg aaaacaggcg gttattcacg cccccgagga tctgaccgtg
     8581 tccggcgacc ctgataaact cgcgagagtc tttaacaaca ttttgaaaaa cgccgctgca
     8641 tacagtgagg ataacagcat cattgacatt accgcgggcc tctccgggga tgtggtgtca
     8701 atcgaattca agaacactgg aagcatccca aaagataagc tagctgccat atttgaaaag
     8761 ttctataggc tggacaatgc tcgttcttcc gatacgggtg gcgcgggact tggattggcg
     8821 attgcaaaag aaattattgt tcagcatgga gggcagattt acgcggaaag caatgataac
     8881 tatacgacgt ttagggtaga gcttccagcg atgccagact tggttgataa aagga
//
LOCUS       DAZUKC010000092         8864 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000092 DAZUKC010000000
VERSION     DAZUKC010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.094"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            15..314
                     /locus_tag="ACG80T_002303"
     CDS             15..314
                     /locus_tag="ACG80T_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841927.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(411..845)
                     /locus_tag="ACG80T_002304"
     CDS             complement(411..845)
                     /locus_tag="ACG80T_002304"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4841928.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1077..1787)
                     /locus_tag="ACG80T_002305"
     CDS             complement(1077..1787)
                     /locus_tag="ACG80T_002305"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFS4841929.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1777..2637)
                     /locus_tag="ACG80T_002306"
     CDS             complement(1777..2637)
                     /locus_tag="ACG80T_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFS4841930.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2642..3280)
                     /locus_tag="ACG80T_002307"
     CDS             complement(2642..3280)
                     /locus_tag="ACG80T_002307"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFS4841931.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3295..3594)
                     /locus_tag="ACG80T_002308"
     CDS             complement(3295..3594)
                     /locus_tag="ACG80T_002308"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4841932.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKEVTVIGIKNVMSAKEISEKFAETKWYQKNK"
     gene            complement(3637..5121)
                     /locus_tag="ACG80T_002309"
     CDS             complement(3637..5121)
                     /locus_tag="ACG80T_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFS4841933.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPEGYFLIVEDYDTYAEKQRV"
     gene            complement(5141..5602)
                     /locus_tag="ACG80T_002310"
     CDS             complement(5141..5602)
                     /locus_tag="ACG80T_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4841934.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGEDEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5620..6687)
                     /gene="ulaG"
                     /locus_tag="ACG80T_002311"
     CDS             complement(5620..6687)
                     /gene="ulaG"
                     /locus_tag="ACG80T_002311"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFS4841935.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADSKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6905..7675
                     /locus_tag="ACG80T_002312"
     CDS             6905..7675
                     /locus_tag="ACG80T_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS4841936.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7948..8775)
                     /locus_tag="ACG80T_002313"
     CDS             complement(7948..8775)
                     /locus_tag="ACG80T_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4841937.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2696 a         1865 c         1389 g         2914 t
ORIGIN      
        1 aaaggaaata aaacatggat tatactgttg aatttgacag tgaactactt aagcaatttc
       61 cacaagaaga acatttcatg tataaagaag caatttccca caaagtgaca gagtatttgg
      121 agcataattt ttttcgtata aagccagtgc gtactcgttc ggcaaaagga atatacgaga
      181 tgaaagtcaa aattaataaa aaaaattatc gcatagcatt tgctttaaaa aaaaatcatg
      241 tgttcatttt ttatattagt tcgaatttgc aaaaaattgt atttgataaa gaggtgagta
      301 aaaaaataaa atagctgagc attggaggat agattgagtg atactaagtc tatcctcttg
      361 tttttctaaa gaactaagct agaatttttt cttttgtacg ttattatatg ttatttgtca
      421 ttaccaaaaa gcaccttttt tcttctagta tctatgacat aagaaatacc acccatttcc
      481 tttacttgaa caaatcctac tgattcataa aatttttttg ttttctcagt atcatctgtc
      541 aataattcga tttggactgg tccataacta gtgatcttct ccaaagcttc attcaaaagc
      601 actttgccta ttccttggcg ctgatacctc ggatgaatga ggatatcttg aataaatgcg
      661 atcgtcgcaa aatcagtgat ccaacgaacg attccgataa tcttcccttc cccattacgc
      721 tttatcagag attcagattg ttcaaatgct ctagctaacc ttgcagtgtc ttttgtgtaa
      781 ttcgtccaac caacgctctt atataattca gaaatctcat cgatcataag ggaatcaata
      841 ttcatttttt tcttcctttc ttatacaatt ctccctctaa taatacagtt ctttgactat
      901 ttaatctatc acatataact ctcctgtaaa gattctctcg attccttaaa atctctcttt
      961 ccttttagaa tgcatccttt ttgataaata aaaaggttgt gataaatttt cttcacaacc
     1021 tctgtttctc tctatcaagt gttatctttt aattttctac ccttacccta gcatctctat
     1081 tgcccgtagt atgcatactt tccatgtttg cgaaaataat gtttatctag taaatattgc
     1141 gggatttcac atatattggg gttgatcagc tcgttttctt tggccatcag acaaatttca
     1201 tctaagatca aactgttttc tactgctttc ataggtgaat ccccccatgt gaacggtcca
     1261 tgtccataga caagtactcc agggacttcg tttggatcta attttctttc cttaaacgtc
     1321 tcgacaatca cattccctgt atgaacttcg taagcttctc ttacttcttc tttcgttaat
     1381 tggcgtgtac aaggaacctt accataaaaa gcatcagcat gagtcgtgcc ataagctggc
     1441 aaatcgcgtc ctgcttgcgc ccacatgacg gagtgcgtgg aatgagtatg agtaatagca
     1501 gttatatctt caaaagtttg atacaaaaca acatgtgtgg ggagatctga agatggtttt
     1561 aagctatctt cttctattat atttcccgac aaatctgtta ccaccatttg atctgcttgc
     1621 atgcattcat atctgacacc gcttggctta atgacgatga ctcctagctc acggtccact
     1681 tcactcacat tgccccaagt caatttgact aatccgtatc gcggcaatgc cagatttgct
     1741 tcaaggacac gttgcttcat tgctgcaatt ttttcttcac aagacatcat agccagcctc
     1801 cttcaattta gaacttaagt agtcttttgc ctgctggatt tctcttcgaa agtcttggct
     1861 tttttcactc cacatttcaa tcagaaatgt tccatcataa tctagacgtt tcaaatgtct
     1921 taataatcca agaaaatcaa cacaaccgct accaaaagga acatcacgaa actggcctga
     1981 atattctttt gttacagggt aagtatcttt taagtggata gcagtgatat aatcgatccc
     2041 tttctcaagt tctcgagccg gattattttc cggccatgcg gacaaattgc ctaggtctgg
     2101 atatacttgt agaaaagggg aatgaatctg ttctttgatt tctaaaaatt ttgaaatgga
     2161 gttcataaat ggatcatcca tgatttccac ggaaagaatg atgccatatt tagaagcttc
     2221 ctttacgctt tcttttattc cttcgataaa gtcttctctg gaattcattg atttctcttc
     2281 ataataaaca tcgtatccag ctatttggat tactttgacg cttagattat gcgccaaatg
     2341 gattgctttt ttcattagtt gtttcgctgc ttttttcttt tctggatcag cagaaccaaa
     2401 cggaaatcgc cgatgcgcac ttaaacaaat cgaatggata cgtacaccgg agctgaacat
     2461 ctcttctctc agctcagcta tcttttcttc agaccaatct aatcgtgcta accgctcatc
     2521 cgtctcatca atcgacatct cgataaaatc gaagtccatt tcttttacaa gggaaaaacg
     2581 ttctttccat gaaatatctt tgggtaatgc tttttcataa atcccaattt gagccatttc
     2641 cttaccccca gatacgaatg atctcatctt tgaagttttg tgcggccttc atcggatcat
     2701 ttgctgctgt aataccacga ccagtgataa aggtatacac atcgatccct tcaaataagt
     2761 gtaatgtgtg tggatttaat ccaccagtta cagaaacatt aaagcctagt tcaatcagtt
     2821 ttttgatctt cgataaatct ttttcacccc aagtttcacc agataacagc gcatcacggc
     2881 tttgatggta gattacctgc cggataccga tgttatgcca atccatggct tgttcaaaag
     2941 tccaattgcc gtagagttct acttgcaatt cacctatttc tttttgtgct gcctccatag
     3001 ttggaatcgt tgcacaacaa atgactgtca tccagtctgc tcctgctttt gcaacattac
     3061 tcgccactgt tcctccagcg tctgcacatt tcgtatcagc gactagtttt ttatctggga
     3121 acatactgcg aatacagcga atcgcttttt gtccttcctg taagcaaaga attgtgccca
     3181 cttcaataat atctactaca tcaccaactt tcataacatc tcctaatgca tgttctaagt
     3241 cattgtgatc taacgcaatt tgcaaattcg gcctgctcat cttagttcct tcctttactt
     3301 gtttttttgg taccactttg tttcagcaaa tttttcactg atttctttgg ctgacatcac
     3361 atttttgata ccgataactg taacttcttt tttctctgcc tgagtaaaca tattcaaaaa
     3421 attgactggc gtaaaaacta catcgtattg gccagctgaa ctttttccct ctgaaatggc
     3481 acaatgatgg attttggtaa ttggtacgtt tagttctttc attgcttttt ccacacttct
     3541 catcatcatc aagcttgttc ctgatccatt tgcacacgat actaaaattc tcatcttaat
     3601 ccctccatta tgagtgttgt ctaatcagat tttcttttat actctctgct tttctgcata
     3661 tgtgtcatag tcttctacaa tcaaaaaata accctcagga tttttccgat attgtaactg
     3721 cggaatagcc aataaaccaa tgacaactac ggcaattcca gcataaccta ggactttcat
     3781 gattactgta aacaatggcc aagctgttgc ccagtcgaac atcccgatat aaccaccata
     3841 tgttgacatc ccaatccatg tagcgaataa tgctgaaccg cctacttgta tgattcctga
     3901 aagaaacggg aacaggcaag cggctttgaa tccacctcgg ttattggcat aaactgctat
     3961 aacggcatta tcaaagaaca acggaatgaa accggctatg acgatcgttg gtgatttcaa
     4021 taaaatcagt aaaccaatca tcaaaaactg tcccaaagca ccaaacaaga acccaatcgt
     4081 taccgcattt ggcgaaccaa agccaaaagt agcagctacg tcaattcctg gaacagctcc
     4141 tggtaaaatt gtgtttgaga ttccttggaa agattgagtt agttcatcga cgaatgtccg
     4201 aacacctaat tgcaagatcg ccagatatac cgcaaaattt aaagaagtcg tcataatgta
     4261 gaataagaaa ctctgtcctt cttgcatgaa ttgcgcttga atcaaataat ctttgcctag
     4321 tacaatcaga atgatcccaa agaaaaatag catgagtaac gaagtagcaa ccatattctc
     4381 attgaaaatc gacaagaatc ctggtaattt gatatcttcg atttttttgt cttgcttgat
     4441 tgatctattt ttcttttcat cccgcttttt catccactca gctaattttg caaatataaa
     4501 gattccgaac atttgctgat gtgccactgc aaatccggca ccttctgtca attcctgcgt
     4561 gattcctact gttaagttag aagctactgc ccaataacaa cctaaaatca gtcccataaa
     4621 aatcaatact tgtatcctac ctagatcagg aaagcaaaac agtaaaatcc aaaaagcggt
     4681 agctgcttgt tgaacctgaa cgtttccggt agtgaaaacg gaacgcagtt tcgtatattt
     4741 ctgaaaacgg actaataaga tatttaaaac aaatgcaatc agtaataaaa tcatcacatc
     4801 tccaaaggta cggccaaatg tctccattaa tccagcatcc acagcatttt gtccaaaata
     4861 aggatctgta accatggcat ccaaattgaa ccgttccttt agtccaacca aaatcggccg
     4921 aaaattattg accaaaccac cagagccaac agtcaaaata aagtaaccga ccgttgcctt
     4981 taagaatcca gcgatgcatt catgaagcgg gcgtttcagc aaaatatagc ctaataacac
     5041 gataaaacca atcaggtatg ctggctgcgt caagatattt gttgcaaaat acgtccaaat
     5101 gcttaacaaa aattccccca tcttttttct cctctatcaa ttaattatat tctgccatca
     5161 caacttggta atcatcgatg gagttcgtag agatgagacg ctcaaccaat ccatctgtca
     5221 ttaataattc ggataatttt tggatatttt ctagatgttc ttctggatct tttgctgcaa
     5281 gtgtaaaaaa caaactagcc gttttctcct catcctcccc ttcactttca aaacggattg
     5341 gctgtttcaa cttggtaaat gaaatagctg ttccaaaaac accatcactt ttttctgaag
     5401 aatgcggcat tgctacttga ggtgcgatca caatataagg accgtatttt tctacacaat
     5461 ggatgatctc ctccacatac gtttcatcaa taatttcctt atcgatcaat acaagacagc
     5521 tttcggcgat agcttcacgc caatccgtga tttctttatc tgggtaatga atcaattgat
     5581 ggtcaaaaaa gtatttcagc aagaaactcc ccttctttct tataaaaacg acggaaacgg
     5641 tgtatcattt tcaatcgaga agacatcttc aaatcctcga taaaaattaa actcaagatc
     5701 atctttgttt gtcggataga tgaatttccc gcctacttgc cagatataag gtttaaatcc
     5761 atacttcagg cgatcctttt tgaatttcca aatttcagtg atctcttttg aatcagccat
     5821 aaaattagac caaatatcgt agtggacagg aatcactact tttgcattca atgactccgc
     5881 catgcgcagc atatcaacag aagtaacttt gtctgttatc cctcgtgggt tttcaccata
     5941 ggcccctaaa cagacatcga ttttgtgttc attaccatgt ttggcaaata aattggagta
     6001 atgtgaatct cctgcatgat aaagattccc tcctgatgtt tcgaataaat agtttactgc
     6061 aatttgatcc atctcctgag gcatttttcc ttttaatacc tcttctggat cttcacaagt
     6121 gaccaacgct gtccgatcaa acgcttctaa agctactaca ttgatgtctt tgattttgac
     6181 tgcatcacct ggctttactg taatcgtttt ttcttcagga attccccatt tttgccacgt
     6241 gcgaaccact tcttttggcc caatgaatgt tgcttcagga caattttgat gaactgctgc
     6301 ggctgtatgg atatctagat gatcggaatg gatatgtgta acgattaaag cgtctacatt
     6361 ttttatcgca aaaggatcga ttacgaaagg ctgagtacgt aaattaggct gcatattttg
     6421 aacgccactc atacgcatca tctgatggcc ttttttcatc atgccattcc catgactacg
     6481 ttttcccgtc ccacaccata gatcacataa aatattcgtt ccttcatgac ttttcagcca
     6541 aattcccgta cagcccagcc accacatagc aaaagttcca ggatccacta cttctgtttc
     6601 gatttcttca ttcaagtatg tgccccactc aggaaatgtt tctactaacc acttttcttt
     6661 tgttacttca ttgacagttt tactcatttg cttgctcccc ccatatcgtt gttccaactt
     6721 acaagtaaat attaatgtgt atcagcatcc atggaaaccc ttttatatga ccgtttgttt
     6781 tttatatgat tttctttcag taatcaatga ttgtttcagt ataataaaga cagatatgaa
     6841 tgtttaaatg aacaactagt caaataaatc catggatcaa gatgtccctc aggaggcaaa
     6901 agtcatgaaa aactcacttg tcaatattga aaaacgtcaa aaagaaattt tggaactgct
     6961 tcagcagact gaacaactat caactttcga attagctgaa tcccttgatg tatctattag
     7021 tacgatacga agagatttaa atttattaga agaaaaaaac gagatcatac gcaattacgg
     7081 ttattgttct tataattaca aaaaccagac tgattttgat caatctggtc ctgaaaggat
     7141 taaacaagcg attgccagag aagctagtaa atacattaac gattatgata cgctgtttat
     7201 caattctagc tcgactgcgt tgaaaacact ggattttctt caatctaacc atttgacagt
     7261 cattacaaac aacgtgaaaa tcaattactc tccgcacaaa gataattgta gttatatttt
     7321 aactggtgga gagatgcgct ttccaaaaga agcactagtg ggcgacatcg cattaaatac
     7381 gatttcttca gtcaatgcag acatttgtat cattggctgt agcggagtcg atgtagtaca
     7441 tggggtcaca acaaagatat taaatgaatc aacgatcaac gaaatgatgg tcaacaagac
     7501 aatcaaatgt aagattttag tcgctgatca tcgaaaaatc ggactgacat caaagttcaa
     7561 gattgctgat atttcagtct ttgattattt gatcactgat aaattttgtt ctgaaaaagt
     7621 attagaggag atcagaaata cggggataaa aaccattcaa gtagattcat tttaagaacc
     7681 aggacgtaaa tatgtaaata ggattatcaa aaaaatgaat aacaaattta tcgataaatg
     7741 aaaaacacgc tacgctcaaa ttgataaagg aatcccaaga tgtgtccatc caatcgatac
     7801 catcataatc tattgcttct tttataaccg ttatatgttg cctaaatagg aatcaaacaa
     7861 tatcgaaaaa aggaggtatg acaaaagtca cacctccttt tttcgaataa acggtattca
     7921 aatcagaaca ctgatttcac aaccacttta tttcaggtcg ttttcaatca cctttaccgc
     7981 gtaatcattt ccgttgaaac gcgctatttt ttttgcgtcc atatatcttt ctaaataatc
     8041 atcttctcct aattgcttat ggcaagaagc aatttcataa agcaagtatt ccaatgcata
     8101 agtcgattta tgtttcttat tatactcaat acccttctca cataattgga ttgctttatc
     8161 ataatcctcg attttacggt aataggtggc gatatcataa tatacaatgc agaccatcag
     8221 attatcaaaa tcagtctcac tcaatatttt aagtgtacgc tccacataaa ctttcgcaaa
     8281 atctaattct ccttttgaca agtagatcat tgccacgttt gcagctgctt gtaagtgatg
     8341 gatatctgtt tcggtcgttt gttccaacac ttgattaaaa taaaataatg ctgcatctgg
     8401 ttttttcgca taaagatgat caagcagtcc ttgataaaat aaaatttgat ttaaaagatc
     8461 agaattactt actttttcct gatcgatcgt ttgtaaaatt ttataagatt cttggtattt
     8521 tgatacagaa ttcaatgatt gaatctcatt tatttttttc ctgattttgt cttcttctgt
     8581 ttctactaat atatcggaga tctcgacatt cagctttttg caaagcgcac ttaagatatt
     8641 gatgcctggc gacatattct tgttttcgat attgctgaca gttgcttgtt cacaaatgcc
     8701 ttctgctaat tgtacttgtg ttaacccctt tttctttcgg tattctttta gtaatgctcc
     8761 atctacttta atcaaaacaa tttccttccc tttaaaaaat tatttctcct ttatatcata
     8821 cacaaaaaaa agttctaaac aaccaaaaat gtttagaaca gaat
//
LOCUS       DAZUKC010000093         8856 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000093 DAZUKC010000000
VERSION     DAZUKC010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.095"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(471..695)
                     /locus_tag="ACG80T_002314"
     CDS             complement(471..695)
                     /locus_tag="ACG80T_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841938.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            886..3663
                     /locus_tag="ACG80T_002315"
     CDS             886..3663
                     /locus_tag="ACG80T_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4841939.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3744..4163
                     /locus_tag="ACG80T_002316"
     CDS             3744..4163
                     /locus_tag="ACG80T_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4841940.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4175..4645
                     /locus_tag="ACG80T_002317"
     CDS             4175..4645
                     /locus_tag="ACG80T_002317"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4841941.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4662..5441
                     /locus_tag="ACG80T_002318"
     CDS             4662..5441
                     /locus_tag="ACG80T_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS4841942.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5428..6249
                     /locus_tag="ACG80T_002319"
     CDS             5428..6249
                     /locus_tag="ACG80T_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4841943.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6266..7315
                     /locus_tag="ACG80T_002320"
     CDS             6266..7315
                     /locus_tag="ACG80T_002320"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS4841944.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7328..8332
                     /locus_tag="ACG80T_002321"
     CDS             7328..8332
                     /locus_tag="ACG80T_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS4841945.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
BASE COUNT         2957 a         1466 c         1833 g         2600 t
ORIGIN      
        1 atcttttttg ctacgtaatt tttgtaaaaa agtgggttga gagaaatcta attgattttc
       61 aaagaaggag tatgaaaatg agaaaaatat ttattcgttt attgtgggta ctatcttaga
      121 ggaataaata atagtggaga aaactatggt ttggcgcaga ttctatcagg tttgatggaa
      181 cgaagacaac ctcccgacat caagtcaaaa gctacttgag attttgatct gaaaataaaa
      241 aaagaaaaca aggtaggaac atcaatgttt ctaccttgtt ttcgtaatca atgggaaaaa
      301 actaagagtg gtgtacgtaa tcaataaaaa ggatggtgac aacagtacct ctttactctc
      361 tgaaagcatc ataactcgtt tttcctcatt atgccaataa attgttgtta taccacatat
      421 aaggatttgt aatagctaaa aacagacagg aaagttgaaa tctgtgaatc ttattttgta
      481 tggaaggatt tatagaacgc aaaaatcaag ataaagatga ataaaaatcc tgtttcggct
      541 atatattgcg tatctacgcc aataaaacga gtttgttctg tcatagccat ataaagcaaa
      601 ggcaaaaata caactacagc gagataagat ccgatgattt ctcgaaaagt tttccaggaa
      661 gtatgtttcc tgtttttata tttatccgat ttcattgaat agtcatagcc tccttattct
      721 ttcatatgtt gaaattccag tataaaaact ggaaggaact gtacgttttt tagaataaaa
      781 attataaggg ataagtaggc tgctgtctat aataagatat atttttcaat tctttattaa
      841 agattttatt gacaaaatga atccgttttc tatgatggag gaggggtgaa taagttgttg
      901 aagaaggaaa ttttatgtta cttagagaac cagacagcct ttttcgattt agaacatatg
      961 aacgaggtct ttacagcaca aaggttagct gatcattttg gggtaaaaag aaacacagtc
     1021 agtcattatt tgaaccagtt aaatgatgag caactactag tcaaaatcaa ttctcgacca
     1081 gtgatttatt tccacaaaga agcttttgaa aaacaatttt ttaagttacg cacgaatttt
     1141 tatttttcta tcgatgatct caaagaggaa cagccttttt tcgcgcaacc aaaagactta
     1201 ttctctttta tgatcggata cgacgccagt ttgaaagaaa gtatcgaaca aatcaaaaca
     1261 gctctttact atccagatgg cggattacca cttttgatta caggcgaaag tggcacaggt
     1321 aaaagttatt tagttaattt ggtctatcag tattgtctgc ttcatgatct gctagaagat
     1381 tctgcaccat tcatcacttt taattgtgca caatatgcag ataatcctga acttttgact
     1441 agcaatttgt ttggacatgt taagggagcg tacactggtg cagaagaaaa taaaaaaggg
     1501 gcttttgaag cagcagatgg tgggatcttg tttttagacg aggtacatcg cttaacacca
     1561 gaaggtcagg aaaaactgtt cacctatctt gatcaaggtg tgatttatcg tatgggagaa
     1621 cctaatttgt cacgccggat caaaacacgt ctcttttttg ctacgactga agagattact
     1681 agtcatttct taacaacttt cattcgaaga atacctatcc gaatcgaact acctgcactt
     1741 aatcagcgta gtcgtaatga acggctagaa ctgatttact ctttttttct cgaagaacaa
     1801 agaaaaatgg ggagcccttt gtcagtcagt ggaccagctt tgtcactttt aacaagtcag
     1861 aaattatccg gaaacatagg agagctgaaa aatatcgtca aagtttcagc agcaaaagca
     1921 tatgctgaac agcgtgaaca gcctgaaatc catgtcacga tccatcattt gcaaaaagaa
     1981 ctacttgcgc agcctattac taaaaataca gcacagcaag gaatctatat tacgcaagac
     2041 cagaccttag agcaattaat ggagaagcaa cagcccgaac agcagcgaat cgttaaaagt
     2101 tttgagcaaa tcctaattga tttcaagaaa aataactgtc tgctatattc ttgcgaagga
     2161 aaattaaagc aagaagtgat tcaattattt gattttttgc tgtttgagac cagtagacaa
     2221 gaaaaacacg agctgctggt ttattggaca caatctatcc gagagacctt tcgacaaatg
     2281 gaaactgcct atcaaatcaa attcgatgga aacagtgtct atgccttgag ttactatctc
     2341 tatcagcgaa gcactatcaa atggttaccg gaagaacatg atattatcca actaatcaaa
     2401 gaattagaac aacaagtttc tcagtcttat ccatctagtt atcactatgt ccaacgaatt
     2461 ctggaattaa gtaagatcaa attagatatt gaagacacaa gtatggatcg gattttactg
     2521 acgatctatc taaaaaaagc gaaatggtca aaagaaaaac gactgccaaa agcgatgatt
     2581 gctgctcacg gttatgcaac tgctagcagt atggcaaatg tagtaaatcg tttattacaa
     2641 gaggatctat ttgaatcatt tgatatgcca ctagatgtca caccacagca gattgcttca
     2701 gaaatccttg attattgcga aaacagcgat gtttcaaatg gattgatcat attagtagac
     2761 atgggatctc tgaaagaaat ccatcagttt tttaaaaaac agttatccgt accgcttttg
     2821 attttgaata atgtgacgac acctttagca atcacagtag gggaatgcct gcaaaaaaat
     2881 gtatcattag aagagatagc agaagagact gttcgacaga tccagccaga atggcgactg
     2941 ctctatccag aggaaaacaa acccaaagca ctcattacta cttgcttcac tggaatcggt
     3001 acggcggtcc acttaagtga actgctagaa aaaagcttac ctacggcttg tcagttgaaa
     3061 atcatcccat atgaatatca gcaattaaaa gataagaaaa acagtgaccc attattttcc
     3121 atatacgaag tcgtaggaat gattggcacc acagatcctg atatcaccaa tatcgactat
     3181 ctttcattag aagacctcat ctcgggggaa aagatgtctg tactagctga atggttggac
     3241 actactatga atgcttcaga gaaggaaata ttcaatcagc gcatcattcg taatttttca
     3301 ttggaaaaag tactggattc cgtgactatt ctagatacag agaaggtgat gggagaaatc
     3361 gatcatttta tgagagaatt agaggtgcag ttgaatcgaa gcatcagcaa tcccaaaaaa
     3421 ttagcgttgt atgtccatgt gagctgtctc attgaacgat tgatccgaca aatgccaatc
     3481 gaaacctatc aaggattaga gaaatggaag cagtgtcaaa aagaagggtt atctgctatt
     3541 aaatctgcgt ttagtgtcat tgaagagcat tatagtgtca tgatccccaa ttctgagatt
     3601 gcctatatct atgatatttt gagtgaagat acagagtttc tctcgataga agaagaattt
     3661 tgaacataag caagttgttg ccactaattt tggcatgcaa cttgcttttt tagtatgtgt
     3721 aagtgaaaag aggagatgaa aaagtggata gagaaattat cttagcttcc catggaaaat
     3781 ttgcttcagg gatattagat tcattagagc tgatctatgg gaaaaatcat tctattactg
     3841 tattcgattg ctatactgat gagaattttg atttagcgga aacagtaagg cagcttttta
     3901 tgaggtacaa agataaagaa atcattgttc tgacggatct ttttggcgga agtgtcaaca
     3961 acgaattctt acaatatatc aatcgggatc atgtctatct cgttgccgga ttgaatttgc
     4021 cactattgat tgagctagta tctcggttgg acgctgaagt agagacaact gttttgatcc
     4081 agcaggtgct aactgattca aaaaaaatga tccagttttg taacgagcgg ctcaatcaga
     4141 aaatagaaga agacgaattt taggaggaag aaaaatgatt gtattgacaa gagtcgacca
     4201 tcggttatta cacggacaag tagctttttc atggacacaa acaataggcg cagactgtat
     4261 tttgattgct aatgatgatg tacctacgaa tgagatacga aagacaacga tcaaattagc
     4321 caagccacaa ggagtcaaac tagtgatcaa atcaattgat gactcgattg ccgcgttgaa
     4381 aagcggggtg acagataaat ataagttatt catcgtagtg gaatcgatcg aggatgcata
     4441 taagctggca gaagcgtatc cagaaatcaa aaccatcaac ttaggaggga tcaaggcaaa
     4501 acctggtact cgcagcatcg gaaaagcggt aaatatttta ccagaagagg aggaacgctt
     4561 gaaacgatta attgacaaac aaatagaagt agaaatccgt caagtaccag cagataaaaa
     4621 agtggatgta agagaagttt tgtaagaaag gaagaacctc aatgattgta caagcagtct
     4681 tattaggaat tgttgcattt gtcgcacaat cagaatatgc actagggaca tcgttgcttt
     4741 ctcgtccgat tgtgacgggg ctgtttacag gaatcgtgtt aggtgatgta aaaactggga
     4801 tcattatggg cgcaacttta gagttagcat ttatcggatc gttctcagta ggtgcttcga
     4861 ttccacctga tgttgtgacc ggtgggattt tagggacggc ttttgctatt acagctggag
     4921 ccggtacaga aaccgcgctt ttactaggtt tgccgattgc tacgttgaca ttgatattga
     4981 aaaatattta cttagggctt ttgattccaa ttatgaacca taaagcagac aattatgcag
     5041 aagaaggaaa ttataaagga gtcgagcata tgcacttgct tgccggtatc ggtctttcct
     5101 tgatgcttgg tgttgtggta atggtttctt atatggtggg aagcaatacg atcggaaatc
     5161 tattaaatat gattcctgac ttcgttcaaa aggggttgtc agtagctaca gggttgattc
     5221 cagctcttgg ttttgcgatg cttgctcgtt tgctgatcaa caaacaagtt gcaccatatt
     5281 ttttcttagg ttttgctata gcttcttact tagagattcc tgtgaccgga gtagctattt
     5341 ttggtgcgat tttagcagtt gtggtagtca atattatgaa tgtccgtcaa ctaccacttc
     5401 aaacaactgc aggggaggta gaagaagatg aagaattctg agatcaaaaa cagcaaaata
     5461 acgaaaaaag agctaaatca cgtattttgg cgttcttttc aaatggagtt ttcttggaat
     5521 tatgaacgtc agatgaatat ggcttatgtt tatgcaatga ttccgatttt gaaaaagtta
     5581 tatcagtcga aagaagaaat ggcagcagct ttgaaacggc atctagagtt ttttaacacg
     5641 accccacata tcgttacact gattttagga atcacggctg caatggagga agaaaataag
     5701 gatgatcctg aatttgatgt gactgcgatc gatagtatca aaacatcttt gatgggacct
     5761 cttgcagggt taggagattc tttcttttgg ggaactttgc gattgattgc cacaggtgtg
     5821 ggtacctctt tagccatgca aggaaatatt ttaggcccta tcttgttcat tttgattttc
     5881 aatattccgc acattctgtt tcgctatcta gcaacaggtt ggggatatcg cttaggaacg
     5941 ggttttttga aaaagatcca ggcaaacggt atgatggaaa gtttgacatt aggcgcgtct
     6001 attattggac tgatggtcgt tggagcaatg actgccacga tgatcaccat caatattcct
     6061 ttaaaaatcg gttcaggaga aaatgctgtg actgttcaaa gtatttttga tgatatcgtt
     6121 ccaaatatct tatgtcttgg agcatttggc atagtgtttt atctattgaa gaaagaagtg
     6181 aaaccattaa cgatcttgat cggactagct ttctttggaa tttttggttc gtggataggg
     6241 attttttaaa aaaggagcag agaaaatgtt tacgatgcaa gattatatct atgaagaaaa
     6301 ggaagtacta agtacgatct tgaaaaagaa tgatttttcg acaagagaac atatgaaaaa
     6361 aacagtgaat ttactgatct tagcaacggg ttcatcttat aacgcttgtt tagcagcgaa
     6421 gccagcattg gaatcttatg gtgatcttac tgtcgatatt caagaacctt tttatttttt
     6481 caattatgga aaactttctc cttcaatcga tactgtgatt gctgtgtcac aaagcgggaa
     6541 aagtgcttct actgttgaag cagtaaaaat gatacaaaaa caagggttac ctgttgttgc
     6601 tatcacaaat gacgtgcaaa gtccacttgc tttagaagct gcacaaatta ttgatttagg
     6661 tgccggggta gaatcagtag gcttcgttac aaaaggctat tctgcgactg ttttacagtt
     6721 actactctta gggataggaa ttggaataag taaaaataaa atcagcaaaa aaatagaaca
     6781 agactacatg caacagttgc gcaaaataat caaccattta ccagcgatta ttcaaaaaac
     6841 ggaagaattt tttgatggat atcaaagttt atttcgttta gcacagcgtt tcgtcaccat
     6901 tggatatggt ccaaactggg gaacagctaa agaagcagag acgaaattga ctgaaacaat
     6961 ccgcgttccg tcacaaggat ttgagttaga agcttatatg cacggcccat acctggaggc
     7021 agatgcttcg catttgctat tctttattga aggggaaagt gtaaataagg aacgttcgca
     7081 aaagttgcaa agatatatga gtcggtacgt tggagagact ctcaccatta ccacaaaaaa
     7141 agcaagaaac gagaaaacac ttggattagc aatagaatgt gatgaatatc tgtctgtatt
     7201 agcgcttgtc gttccgtttc aactattcgc ttataaaacc gcagtagcta aaggtattga
     7261 cttgaacaaa aagatttttg aagactttga tacagtcttg aaaagtaaat tataagaggt
     7321 ggaaatcatg ctgaaattca atgaagaaga acaaatcaaa gcaataaaag gcgcactagc
     7381 tttacgtccg caagtagaag aaatcatcga taaactttat gcggaaaaat ttgacgctgt
     7441 ctattatcta ggaatcggtg gaacgtatgc ttcctcgatg caagctgtca cttatatgaa
     7501 tgggaaaagc aacttgcctg tctttgtaca gcatgctgca gaatattaca ctactggaaa
     7561 caaaagatta acaaaagatt ctatcgttat tttgtcctct gttaccggta caactcaaga
     7621 agtagtgaaa gcagtagaac aaatcaagaa agtaggagcg acactgattg gatttatcga
     7681 taaagcgaac agtaagttgt ctcagctatg cgattttgtg gtcacttatc cagctcctgg
     7741 aacagaacaa atcaaatttt ttatggcagc agatcgattg atgtatttga atggagaatt
     7801 cgaagcttat tcagaatact atgaacagct cgaacgttat ttaccaacag ggttagtgga
     7861 agcagagaaa aaagcagatg catttggctt atcatttgca gaaaaacatc gacgtgatag
     7921 tatgcattat tttattggag cagggaatca atggggagct gtttattcat atgccatgtg
     7981 ttactgggaa gaacaaagct ggcttccatc gaaatctatc catgctgccg agtttcttca
     8041 cgggacgtta gagatcgtag aagaaacaac cccagtcaca ttatttttag gcgaagatga
     8101 acagagagtg ttatctgaac gtgtggctaa acttttgcca aaaatttgca gcaattatac
     8161 attgatagac acaaaagact atccagtaga aggtatcagt gaaaaatatc gcggacgtgt
     8221 tttgtctttc ttattgatgc atgtagtgac acaacgcatc gatgctcatg tggagcagtt
     8281 gaactgccat ccattagaga tacgccgcta ctatcgtcag tttgattatt aggatagact
     8341 ggataaaaag aatataaaaa ataaagagag cagcgataac tgctcccttt atttttttaa
     8401 aatattggca tcaattacgt tataaaacac attttttgca ttatctacag ttcaacttat
     8461 aatctgttta atgggacatg tgggtgccaa ccattttttg ttatgcaata gtactattta
     8521 gttgttgcat ggatccgtag taaaagtcca agtacaacga gttgcgccag tttacatagc
     8581 gttttgtatc cagaatgttg actaaaacaa aagttcctat gtcaaccgtc aacagagaca
     8641 atctgtccgt tgcaggacct gacagattta gaaatgttca atctgtctct tatgtatttt
     8701 aaatcaatac tcactaggag aagtttccta acctcctaat agtaatgctg gtagtaacat
     8761 tgtcaaaact gcttcctctt aggaacaagc aaagcataac gagtgtaaaa ataaaatgta
     8821 gactattgta acatatcatt gattatttca agtaaa
//
LOCUS       DAZUKC010000094         8797 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000094 DAZUKC010000000
VERSION     DAZUKC010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.096"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(166..618)
                     /locus_tag="ACG80T_002322"
     CDS             complement(166..618)
                     /locus_tag="ACG80T_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841946.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            complement(625..1173)
                     /locus_tag="ACG80T_002323"
     CDS             complement(625..1173)
                     /locus_tag="ACG80T_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFS4841947.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            complement(1218..1550)
                     /locus_tag="ACG80T_002324"
     CDS             complement(1218..1550)
                     /locus_tag="ACG80T_002324"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841948.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(1564..1821)
                     /locus_tag="ACG80T_002325"
     CDS             complement(1564..1821)
                     /locus_tag="ACG80T_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841949.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(2016..2558)
                     /locus_tag="ACG80T_002326"
     CDS             complement(2016..2558)
                     /locus_tag="ACG80T_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFS4841950.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(2579..3670)
                     /locus_tag="ACG80T_002327"
     CDS             complement(2579..3670)
                     /locus_tag="ACG80T_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS4841951.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(3678..5888)
                     /locus_tag="ACG80T_002328"
     CDS             complement(3678..5888)
                     /locus_tag="ACG80T_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFS4841952.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(5904..8432)
                     /locus_tag="ACG80T_002329"
     CDS             complement(5904..8432)
                     /locus_tag="ACG80T_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4841953.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(8484..>8797)
                     /locus_tag="ACG80T_002330"
     CDS             complement(8484..>8797)
                     /locus_tag="ACG80T_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFS4841954.1"
                     /translation="VNAATFLLVVIFEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCV
                     LALAKLKPDGKKVHFYLWDWFCYMIRMMNGDCVYYNWDKVSNNALEELLFVNKYVYLE
                     D"
BASE COUNT         2732 a         1908 c         1234 g         2923 t
ORIGIN      
        1 ttgaacttta acttttaaaa actttaaaaa taatcttatt tgttcttcta aacaatcatc
       61 tatactcata taatcactcc actaattaat ctccccaaaa agacacagca ttaagagata
      121 taccaacaca acagtgcaaa taactctaca tcttccttcg caaccttatt tcaataaacc
      181 ataaaaatta ttttttaata gaaactgaaa attaaaacta gaaaaagtag ttttaccgaa
      241 tgtaagagca tcatcaccat caatatagaa atacattctt ttccagttac atttaatttt
      301 taaacaaaat tctccccaaa aaggaacttt ttctagtgaa aacttggtaa tctctttgta
      361 attaattctt gttaaagaat aattaaacac ttcattttta cttgttattt tttcattgac
      421 tataactagt tcatctggta aaaaaattaa ccaacaaaca tcgacaaaca gtaaatgata
      481 taatccattt ccacctccaa gcatattcaa tactggtgaa catgccttag aaacacgaat
      541 atagttatgg tttaaataga tattttcttt aaacattgct tgtttaaaag ccatatgctc
      601 ttgttctaaa cttttcataa acttttactc taatgcacag ctagcagcag ctgctgcaat
      661 tcctgctaca atatttacct tagctaactc tttagctgca gcttttccta attgtttttc
      721 tgcaattttt aataataaat ccgcagcttt ttcaaaatta aaagctttta atgcttcaat
      781 aaaatctccc tgcgtaccta tagcccaagc cactcctcct ccaggaagag aattaatggc
      841 tacacatcca gcatatcgtt tccaatcaac tttctccatt ctaggaacaa taatatgttc
      901 ataaaagact cttgctgctc ttccttgttc gttatcttga ctgattcgtt cacgaatagc
      961 accaaaatcc tttatatggt atacaccatg ttcgtctaaa aaaccaactg tatcaaaata
     1021 aaaagttaat tcatctacta atttattctt tatttctaaa tcaactgtat tcactttttc
     1081 tgtggcatac acacttgttc cagtactaac aaaagtactt aaacctaata agaaaacacc
     1141 taaaacacca aatattttaa ttattctttt cataatttct cctccataaa aaataagtta
     1201 ctttttaaat atcttgttta tgaattcatt ccccaaaggc cttttaagat tgatacagca
     1261 gtccgttcac ttcttttaga cttgtcatac aagtctttta aatgatccca tatcagacta
     1321 gtttgtctat ctagcttcaa tccattaaac gctaatgaac ggttcatttt ttctattact
     1381 ttagataaag ctacagaaat ttttatacct tcacaacgcg catcttggat ttcaataata
     1441 gtatcatcaa ttagtttttg aatttcttgc ctatcaacat attcaatctt agaaagagaa
     1501 tcttttaaag ctactaatga ttcaacgatt tcttttttct cttcagacat tttcttcctc
     1561 cttctagtga cccgtaaagc catgtagagc agagtccatt gaaccagtaa ttacactatc
     1621 tacaacgcca ttccaattta ctctacattt tccatttttc agatttgtcg gtccatacgg
     1681 atagcctttt tgacattcaa tgttcggatt ataccagata ggattatacc attcagccgc
     1741 agaagcagat tcagtcgata caaacaatcc cacaccaaca aatccaatac acatcacact
     1801 atagccaatt agttttttca tctcgtatcc atctcctaaa aaatagaata gttcttatag
     1861 ttattcataa gacatatttt ttccgttttt gttttatcct ttctgtccta taacaacttt
     1921 tttcaagtaa agaatgacat agtttttctt aaaaaaacag tctgtttttt tctatttctt
     1981 cggtgtataa taaatatgag tgttcatttt ttattttaat acgcactccg tacataataa
     2041 atcttgatcg catcaatctt ttgagcttct tgatctactt tcaattgaag taatacaaat
     2101 tgagtaacct tattcattgt ttcttctcca aataacaaat attgatccgg attctctata
     2161 tcttgtgtca cctggtaaag tttcccagtt gttttcattt gtacatgcgg gtctacatca
     2221 atactattgt ctttaataca acgatctgtc atatagtccc tcacagcttg attgcgttta
     2281 taaacatctg tgaaattact ccattttcta gcaaaatctt ttaagaaatt ttttgtcatt
     2341 tcatctattt gcatttgttg cgctgtctcg tcttctaatg aaccaatttg actgttactt
     2401 tcagtacttt gaagagttcc acttgtttct tttactgctt cgattgtttc ttctgtcgtt
     2461 ctcaattgtg ttttttgttt ttcagaagaa aaccaatgac aaccagtaag acctatcaaa
     2521 cataaccaac taaatactaa aattatccct tttttcacgt ttctagtcac tgcctttctt
     2581 atttcacctt acgaaccaga ctacgaattt ttgtttgatt gtacgtgcgt tgataccgag
     2641 ccataatccg tccttgctcg gcattctgtt ccacactaga gaaatgttgt ccgccatatg
     2701 aaacatctgt tataattccg gtgtgaccat agattccagg agacaatgca ccacctgctt
     2761 gccaattcac cacatctcct gcttgtaaat cacttggttt tgggttcatg attacttgcc
     2821 aaccataggt tgtccagtta taatcttccc ctattaacat cgcaaacatg tgtccagaac
     2881 ccattaactg tgggccacct aattgagata cataccatgc cgtaccacca taacattccc
     2941 cattatttac ggatcgtcca atgatccgat ccaaaacctt cgcattgcct ttactttttc
     3001 ctgcaataag cgaagttttt tttgttggta ggctattcca atgcttgatc gcaaaagtat
     3061 catattccgt taagttatag gacgttatga tactcattgc tcctcgataa tacggactcc
     3121 cttcggtaat acctgctgcc gcatagcctc cacgacctag aatcaataaa caacttccag
     3181 gatctttatt gcccacacat tgattaaagt tgctttggct cgccaccata aagccataat
     3241 agtcataaca agcttttaag ttcggaaact tcatatagaa accaccttca gaaccaccaa
     3301 ttccacgtgc agaaccttgt gggtagggac aaccaggaaa ccaggtaatc ccaaaaaagt
     3361 tcgtatcgtt tcttccagat tgtgactgtc cataagctga ttcatagcct aattgagaaa
     3421 gaatcacact cggtaataac tgatacttta cagctgcacc aactgctgct tgcgcatgtt
     3481 catctgtgat tggaccacgt cctttaatct ctgaaaaaat cggtaatcct tcggtatatt
     3541 ccccattaaa agcgacagct agacctgtag cttctcctcc atgctcagaa agttctcctg
     3601 aaaaaaagaa tggcaataga aaaaagggaa gaataaagaa aagaagacaa cctagacacc
     3661 ctttcttcat ttcttcctca tcctttcttc gataatggtt tttggactgt tcgtcctttt
     3721 ggtaaagtaa aatcagttcc tggttttgcg tccaaaatcg gcagtgtttt aggatccttt
     3781 tctatttttt ccgattgctc tgttggctta tctaaagaag aaaatgcagg ttgtaacgat
     3841 tttggtgcgg aaatcgacgg tttactttca gacgaacgtt gccccggttg ttgcacagaa
     3901 cgatttcctg atagagtggc taccgacgaa ttaggaacag agtattggtt caataactgc
     3961 tgcattgctg gataactccc atttttcgcc tgatttttca cttggcgaat ctgttgtgaa
     4021 agagaaccag cagctgttaa ttgtggcacg ggtacttttc cagaaggcaa cggaatcgat
     4081 tgaccaacaa aagcgatacc tttatttaac aatggttttc tttgattatt cccttgttgc
     4141 ttactcaatt gtgccaatga ttgtacctgt tgttttggtc ttactgaatt catggacgaa
     4201 tttggaatca aaccatttga aagaccagaa ttccactctg ttttcgattt ggtagaagac
     4261 atttcaggct ctaccccatg ataacgaaca tgacgaccac tataaggcaa ggcttgattt
     4321 gaacgcccac ctagttcaaa gggattaacc acttgcctcc cttttaccga ttcttgtggt
     4381 gtacgttttt gttgaaactt ttgaaatcca tgtatcgctg cagaagcgag tggattcgtt
     4441 gctcgataca cattccctgc taatcctgca cctttgattg ccaaattttg tccagcagtt
     4501 gatagttgac caggcagttg tttcatttga gacaaagaat gacttgctat gtttttcgtg
     4561 ttgtttatag tttgatctgc agctcctaac cctagtttct ttaataaatc tttccgtttt
     4621 acaatgagta aaattgatag gagtgctttg acaaacatat ttcctaaaaa accaaaaaca
     4681 gtattcattg gaattaaatc gtctactatg ttatagatca aaaatgccac caaaataatc
     4741 acactgtaaa aagatttttg aaacatcaag cccaaaaagg actttaaggt attcatgagc
     4801 atataatcaa atactggaac aaaagaagcg agtaatgaaa aaggaatcac aagtaataaa
     4861 cacaacacca acatttgaaa gataaaacga atcatgccaa tcactacaag tggtaatcca
     4921 atacttagat taagcaatgg caccatagaa ccaacgactg ctttataaac agctttagtt
     4981 ggttgaatat agacacgata agccttcttt ttccctctat cttctcctgc agcagtggtc
     5041 acttttgtct caatatcaga taattcttca cttgacgctt ccttggctag aaattcttgt
     5101 ggttcaatct ctgcttgata caattcttct aaatttgttg tcccataatt taacaatata
     5161 tacggatcaa caacagccgc tttataataa agttggcgca ctttttgaat ggcttccttt
     5221 tgtgtcgttg gcactaaaac accattgaca tcttttgtcg ccaattcatc tagactattt
     5281 gtagcagaaa aaataaagcc ttctacctcc acggacaaat catttaaccc tttggtaaaa
     5341 gcttctcctt tactaggtac gttactacca tagccaaacc aaaccattga aaaaatgaca
     5401 acgaagagaa atcgaataaa caccattttt gcgtaatgtt tccctctaaa tatacgaaga
     5461 gtaaatagat ataacacata caagacgaca atccccgcac caataggatc gtatattttt
     5521 gaataaatag tatccgaaaa agtgaaaaac tggtgaatta actcgttcat tgcagctcct
     5581 tcatagagct tctcaatgac aaaatcaagg ccttgccaaa tcaatttact taaactaaag
     5641 aacatattcg tcatcatttt tagaaaatct gtgccatttg ccccaaataa cattttgcct
     5701 aatgaaaatt cactatcaga taaataagaa gaaaaggtat caatatgaaa tttcccaatc
     5761 tcatagatcg attgatccac ctgcttttct gccgcataca ccaatccacc aacaaaaagc
     5821 agtccgaatc caatcgttaa aaaaaacaaa acgcaccatt tcttttcctt atgctttata
     5881 taagccattg tttcacctca tttctattgc atgattaacg tatcctgttc ttctacttga
     5941 gagtgacttg ttttttccgt tgtttgtaat gcagcggtca tttcttcaaa caaactgtgt
     6001 acattgagtt ttcctacacg tccataggga tctaacatcc aacactgtcc tttaatcatg
     6061 tggctcattt cagctaagtt cgcttcattt acttccaaac gtaaatgatt caaaatcttt
     6121 tctcgctcac tgtcttcatc aaaagcaaag acgacaccaa aattcccttt ttctttatct
     6181 gaacctaagt caccaataaa ttgtgtatct aacactaatt gattcatctg ggaacgcccc
     6241 acacgtgcca ttgctgcaat aatactacgt cctgcgttcg aattattaaa tacccatgct
     6301 tctgtaaaat aaatcgctgt tttcttacta ctatctcgtt ttccaaacat ttcacaaaaa
     6361 cgacctaaac taatcatcaa acacaaagac tttcgatccg catctgtata tagttctggt
     6421 cggacattgt catttggtaa tttcaaatct tggatctcta aaatcgttgt tttggcatta
     6481 aagtctaatc ccgggttttc tccataagaa aatcctaaac gtaaaatcga atcttctacc
     6541 gtcaagcgta aaaactcacc atattctcga acctcttttt gttccatttg ttctaaatag
     6601 tcgaccacat gcatcaagcc aacttgttgt ccggcttctt tttgaactaa aacttcttgg
     6661 attcccttta ataccgcccc ttttaaaagg tgattggtac ctaacggact taactcatca
     6721 atcatatcct gggctacttg tttcgcatct actcgggata aacaaacaat tggatctaat
     6781 acaccataat tacggggatt tgtagcatct aacgtgacat aactcatttt ttgcaaatgt
     6841 tctacaaaca aagggtaatg tttctgataa tacgggtctt gaatgagttt cgtaaaccat
     6901 ttgcgctttt ctcctttggg atccacaaat aaactttgta cattcataaa cgaactatac
     6961 aaaaagagca agccaactaa aaaggtcttc cctttaccgg tttttccagt tactgctaca
     7021 tgaggcgcat caaacaacgc acctttgatt ccttccgcaa tggctagaag attcaaaaag
     7081 acaaaacgat ttgacgcata aattaattct tctaatggtg ttttttctcc tcgagaacca
     7141 ccatccccta atgtatcttg ccgaccaata taccagccgg attccatacc taattctgat
     7201 actgctgaaa acaaccattc gccaactgct tctacagttg ttcggtgctt ccaatgtaac
     7261 tgccgtccca atggttgccc taataaacat ttatagaaca attctcgttg atcatttttt
     7321 gcacggtgga tatggataat cgtatgaagt tgttgaataa ccgttgcact attttgacga
     7381 acttcttcaa ccgtttgacc gtatacaata aaacatgcga tccaatcgac gacaggttgc
     7441 ccattatcta ctcgttgctt taaatgtttt actaatcctc gaacagttaa taagcgttca
     7501 ctaggttctt catcattttg aaccaattcc ttatgctctg ttttgaaccg ttccgatagc
     7561 caattcagtt ttaaaccaat acctccctgt cctttcaagc gtggatagtg aattttcatt
     7621 cgcaattctg ctgggaaaga aagatcctga gcaaattgaa atagatcaca atttctcata
     7681 ttttcaggtg tttccccaat aggcaacaat gctaaataac tactccctaa ctcatcttct
     7741 aattgaagaa ggcctttatg ttccttcggt atacgtacat acgttccttg attgtctttc
     7801 agtacttccc aattattttg ttcttctact ttgtgagcca ttcctcgaat atattgcaga
     7861 cgattgagat acaacagctc tttttttact aagggacgaa tagaaaagct ataaagtaat
     7921 tgtgtcactt cttttacata aggatcactt tcagaaaaca aagtttccaa atcatctact
     7981 tctaatccaa ataagcgtgc taaacttcct gctaaatcta accagattag atttattaca
     8041 tcatgaaaac tctccaccgt tgctttttct ttgactttga ttcctaaaat aaatgaagga
     8101 cgatagactt gaccttctcg ttccaacaat gtcactgttt cttttgcata atacgcacca
     8161 attgcttgac tatcgggaga aaatcccttt cctaatgtgt caaacttcgt ccgtaaattc
     8221 attgttcgtg gatacatcac caactccaca tcttcataat ctgctaaaag caacagtaaa
     8281 cgagccatca tttcttttat ttcctgtgtc ttttttggtg tgttttttga aattgttcgt
     8341 ggaaaaacat gatagtaggc ccagatacaa tcctctccat ttaacaaaca atttttcttg
     8401 aatgctttta ctggttcttt tacaagcacc aatgaatttc acctcccaat aaaaaaggaa
     8461 caaccagttg attgtccctt tttctaatcc tctaaataaa catacttatt cacaaaaagt
     8521 aattcttcca atgcattgtt tgataccttg tcccagttat aatacacaca atcaccattc
     8581 atcatacgaa tcatataaca aaaccaatcc cataaataaa aatgcacttt ttttccatca
     8641 ggttttagtt ttgccagtgc taacacacat aaatagggga gtcctatata aaaaacaact
     8701 cctgctggtg gaaaaaaaga aatgacccaa ccaataaatg aacgtaaaaa taaatattca
     8761 aagattacga ccaacaaaaa agttgctgca ttgacta
//
LOCUS       DAZUKC010000095         8754 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000095 DAZUKC010000000
VERSION     DAZUKC010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.097"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..497
                     /locus_tag="ACG80T_002331"
     CDS             <1..497
                     /locus_tag="ACG80T_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841955.1"
                     /translation="SYLIVCLIIIICLHHLFLSKTIPRYSKNKKAEKFCLLLNKFTLI
                     APILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFLMSLKPPKSISLCL
                     VINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPNKNNNFYFY
                     RNKL"
     gene            complement(623..1300)
                     /gene="nth"
                     /locus_tag="ACG80T_002332"
     CDS             complement(623..1300)
                     /gene="nth"
                     /locus_tag="ACG80T_002332"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFS4841956.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1307..2005)
                     /locus_tag="ACG80T_002333"
     CDS             complement(1307..2005)
                     /locus_tag="ACG80T_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFS4841957.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2273..3457
                     /locus_tag="ACG80T_002334"
     CDS             2273..3457
                     /locus_tag="ACG80T_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFS4841958.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3463..3906)
                     /locus_tag="ACG80T_002335"
     CDS             complement(3463..3906)
                     /locus_tag="ACG80T_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFS4841959.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(4132..4419)
                     /locus_tag="ACG80T_002336"
     CDS             complement(4132..4419)
                     /locus_tag="ACG80T_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFS4841960.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4590..5942)
                     /locus_tag="ACG80T_002337"
     CDS             complement(4590..5942)
                     /locus_tag="ACG80T_002337"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFS4841961.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSDPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQEALGEIETAQ
                     "
     gene            complement(6214..6660)
                     /locus_tag="ACG80T_002338"
     CDS             complement(6214..6660)
                     /locus_tag="ACG80T_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFS4841962.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6896..7822)
                     /gene="cysK"
                     /locus_tag="ACG80T_002339"
     CDS             complement(6896..7822)
                     /gene="cysK"
                     /locus_tag="ACG80T_002339"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFS4841963.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(7962..8754)
                     /locus_tag="ACG80T_002340"
                     /pseudo
     CDS             complement(7962..8754)
                     /locus_tag="ACG80T_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
BASE COUNT         2387 a         1780 c         1443 g         3144 t
ORIGIN      
        1 tgagttatct gatagtatgc ttaattatca taatttgtct gcaccatctt tttttatcga
       61 aaacaatacc tcgttattcc aagaataaaa aagctgaaaa attttgttta ttattaaata
      121 aatttacttt aattgctcct atccttgcat ttattatttt ttctgtactt ttaagcacta
      181 ctcttaaagg aaaatttatg gaaagaagct ctcatgcaat gattctcact tttttatggt
      241 tgttgttcac cagaatctat atttttctga tgagcctgaa accgcccaaa tccattagtt
      301 tatgtttagt tataaatggt atttttttat tatcacttat catttttatt acacctttag
      361 atagatacgt cacttattta tataatcctt tggaatactg gacttatttt ataggtatat
      421 tggaaggcat tattttctac atcgggtatt ttcccaataa aaataacaac ttttattttt
      481 atcgcaataa attgtaataa gttaccatcc tagctactga gtatgtacat ttactttttc
      541 atgcaaaaaa gataaacgaa acagcttttc tccataataa caagataatt catacattta
      601 tttgcaatta aagaaaccac gtctacaggc gtggtttctt ttttagcgcc ttttctttta
      661 gacgcatccg atttttccca tcttgacata ttgatagcaa aggacatacc tcgcacttgg
      721 gatttcttgc cgtgcaatga tatctaccaa aaaaaatcag tgtatgatgt gtctttaccc
      781 ataattcttg tggtactttc cgcatcagtg tttcttctac ttccatgacc gtagcatcta
      841 gtttgcagat cctcaggcgt tttgagacac gttccacgtg ggtatctact gcgattgctg
      901 gtatcccgaa tgcatccccc aatacgacat ttgctgtctt tctgcctaca ccaggaagag
      961 acatcaattc ttctcttgac gttggcactt gcccatcgaa acgttcaatc agctgctgtg
     1021 cacacgcttt gatgtttttt gctttatttc gatacaaacc aatcgttttt attttaagaa
     1081 tgatttcatc gattgaagct tctgctaacg catcgggtgt cggaaaagag gcaaacaaat
     1141 ctggcgttgc tttattcact gatacatctg tcgcctgggc acttaagatc actgcaatca
     1201 ataactcaaa tggattattg tgcttgagtt ctccatgtgc ttcgggaaac attccataca
     1261 tcgtttctag agcttccatt gttttttgtt tgcttaacat attccgctac tcctccaacc
     1321 aatttttcag tgagactttc ggcaattctt cttgttgagc tgactgttct acttctcttt
     1381 gaagaagctg ctgttttctt cgtttttggt cttcttccac ttgttgtttt gtacgtagat
     1441 tttttctttc ccacgatagc aaaatgcggt caacgtaatt aaaactataa gcctgattta
     1501 atacggcttc ccgcagtgct agcttcaaaa tttccggctg ataatggtct tcttctagcc
     1561 attgtccgat ccgctggaac tcgatcgaag aaagcgggcg tccaaattct tgttcaaata
     1621 attgatacac tgactggatt tctttttctt gtgttttttc ttcgtatttt ttttcttgtg
     1681 tttttaaata ctcacctaat agatcataga tggggtaaag attatatcga tctgcttttt
     1741 taccgttgac aatcgtcgtt tctatcttga taaatccatt tgttacgaga cgatccagta
     1801 tttgaaaaat ctgatcttgt ttgattccca tatccatagc aatcatctgc aaatcaggga
     1861 aagtatctcc ttcaagctgt gccatatgca actgtagaac aaataaaaat tcttcagatt
     1921 gtagaccgat cctatgataa tttttaagta aaagattaga tatgactgtt tgtccagcgc
     1981 ttaagtaatt ttctaaattc aacatgattt ctcctccgct caatcacagt atacgtaaaa
     2041 aaaacatggg aagcaagaac aggtttcttt tctatttact tcactgatcg gtcagattta
     2101 caatgtcgtc tacttgattt ttgtttgttc atgtttttga tgcgtatctt cacttttcgg
     2161 aataaataaa ttaagtagat ttcgctgttc cttagaatgt tgacaaggaa acaaaattac
     2221 tatacactat ttttgtttcc aagttaacat tattgcataa aggagttttt acatgtacag
     2281 tttcacaaat aaacattcta aagtttttgg atacgtctct gtcttattca ctggattagg
     2341 atttactatc gtcagtcctg tacttccgtt tttaacgctg ccttattctc actccataca
     2401 tcagcaagct ttctatatca ctttgctcat gtctgtttat gctctagctg ccttcttatc
     2461 tgcccccatt ctcggtagct taagtgatca ttttgggcgt catccgatcc ttattatcag
     2521 tttattagga tcaagtatcg gatatttgct ttttggttta ggaaattcga tatggatgtt
     2581 gttccttggt cgtatcattg aaggattgac ggcaggagaa atctccacct tatatgctta
     2641 ctttgccgac atcacggaac ctaatgaaag aaccaaagtt tttggatgga tgggtgcttt
     2701 agtcggtata ggaacaacgc tgggtcctat tatagggggt ctattagctg agttggggaa
     2761 ttctgttcct atatttattg gagcactctt tactttctta aatgccgttt atggctacac
     2821 atttatgcta gaaagccttc caataaaaaa acgttcagta gatttaagtt tctcccatgt
     2881 aagacctttt caccaattac aacaattatt caagatttca tccgttattc ctttattgac
     2941 agcaggtttt gctgtttggt tggcagctgg ctctcttcaa tctatctttt ctcaattttc
     3001 gattgacgcc tttcagtgga aggctgggtt ggttggttta agcttctctt tgattggaat
     3061 cttggatact gtctcgcaat tgttcattat gcctcgattg ctcaaaaaat tttcagaaca
     3121 gcagatcaca agaataggga tgttcagtga aatcctcgcc tatctattca tcacattatc
     3181 tggaattctt ctactgccga ttttgttttt atttgggatt atctgttatg gtttcggtga
     3241 ttctattttt actcctgtat tcaatggcca actatccaat tctgtctcag ataaccagca
     3301 aggtcttgta atgggcggta cccaaagtat acaatctcta tcgcgcgtca tcggcccgct
     3361 aatcgcagga cagctttatg ctgcagcacc atttcttccg acggtattcg gatttttgct
     3421 tatgattttt gcctattatc actacaataa aaaatagtag cttcatttgc tacttcgtat
     3481 aaattctctg tatcgaccga taaaacgaaa tactgtgtca aattcttctt ttgacatatt
     3541 ttcaaataca gtagcatctc gctgtaccca tttttgatgg agttgctcgt gtctattcct
     3601 tattttttgt ccgattggcg tcaattgaaa atagatttct tttttattct cttcgctttg
     3661 ataagtgttt atgatccctc tagctatcag tttttttgtt agcttactga tggctcctct
     3721 agtcatgtaa ctagcattcg ctaattttgt cacgttagga tcagggattt tcccgatatg
     3781 ttcaatcact tctagttcat ttagagtaaa tcctgcaaat tctttttcca tttctggttt
     3841 attacgatga ataatctcat tagctaaatc cagatattct tctattagca gttgtctttt
     3901 atccatttct acaccttctt tttctttcat tatattgttt ttttgtttcc tattcaacaa
     3961 agataaaaat attttgtagg aaaacccaat ctgaaaccaa gaaaaaaagg ggttgacaaa
     4021 gcttgtcata accccttttt ctgattaaaa gatagtaaag agacagtcat cggtaattgt
     4081 gatgattttt tgtcatcact taaggatgag cggccttttc aaaaactgtc tttaaagatc
     4141 atcatcagtt tctagcacat atggctgacc ttcatatgtg tactctgtat tcaatgtgcc
     4201 gtcattttca tctgagtaga tgcgtctact gaaaggaaaa cgatgagaaa cattatcttt
     4261 ggcgtcattt gctggcatcg tgtaatattc ctcttgcgtt tcattcaact gagagagtac
     4321 ttgatctgcc aactcatcaa tattgatcgt taatttcttc gaagcacttt tataatcttc
     4381 taaaaatgca tcttgttgga caaaatattc tttattcatc ttctttctca cctttctttt
     4441 caaaatcaaa taaagctgct tgttctttta tgtaacagct ttatctttgc tgcttattgt
     4501 gacaaaaaat tcgaagaaag acaactaatt gtacttttca caaaaaaaga cagagggtcg
     4561 tgacaaaatt gtgtcacaac cctttttctt tattgtgcag tttcgatttc gcctagcgct
     4621 tcttgtgcaa gtaggttcaa gtaattccat gggcggtcaa aatgtggttg gaagaagaaa
     4681 tctgaaagtg cgagatcttc tacagtcatt ttattttgaa cagctaaaga catggtattt
     4741 gctgattgtg taatgtcgta tttggacatg aactgagcac ctacgatacg attcgttcct
     4801 ttttcataga ctagctccat cattactttt tcagtagttg gcatgaactc tggacgataa
     4861 ttatcttcaa atacagtcgc ttggacttct agatcgttca aagttgcgct ttctttggtc
     4921 acacctgtcg aaccgatcgt ccagccaaat aggtaaagtc ctgaagttcc ttgagtacca
     4981 cgatacgcca ttttatgatc tgtcaagtta tttcccacaa gtaaaccttg acgaacggca
     5041 tttgttgcca aaggaatata gttttgcgtg ccacttggat tgtagttgac tactgcagag
     5101 tctccagctg caaaaatgtc tggatcactt gtctgcatgt attcattcac tttgattgcc
     5161 ccgtttggca gtgtatctac ttttccttcg atcagttttg tatttggacg gaagcccaca
     5221 cataaaatga ccatatctgt atcaaatgta tctgttggtg tagctacttg tttcactttt
     5281 ccttcttcat ctgtgataaa ttcagtaaca ttttcgccta gcgcaagagt aacaccgcgg
     5341 tctgtcaatt ctttttctaa gatatctgtg aatggtttat ctagatattt attcaagatg
     5401 cgatctaatc catcaatcaa tgtcacttgt tttccagatt cgacaaaagc ttctactaat
     5461 tcaataccga tatatccacc accaacaatt actacacgtt ttgcttcttt cgcttgtgca
     5521 ataatttcat ttgcttgatt gtagtttttg cataggagta catttttcgc atcgattcca
     5581 ctaatcggtg ggataatagg ccatgaacca gttgtcatca ctaatttatc ataagaaacc
     5641 gtttctgttt ctcctgtttt cagatctttt gccgttactg ttttattttc tgtatcgacg
     5701 tctgttacat cgtgttccat tttaactgtt gctcctaatg aagataattc ttcaggattt
     5761 gagtagaaca agcctgctgg gtcctttact actccgccga cataaagtgc gataccgcaa
     5821 gataagaaag aaatattgtc atttctttca tatactgtca cttctgcttc tggatgattt
     5881 tttaaaatac tttttacagc tgcagtacct gcatgagtac agccaattac tactactttc
     5941 atttatggtc cctccgatta acattttgtc cttcgacaaa actactataa cagaaataaa
     6001 acggatacaa cagtttaatg cttgtgaatt cacaaaggtt tgtttgtagt ttcattcctt
     6061 ttcaagcata agaacccaat atctacaaga ttcttattgt gataatattc actttgatat
     6121 ctaagattgt gaataaaaga caaaaaaata aaaaagctag cgttttttta cacagctagc
     6181 tttttttagg tataacactt atagaaactg tttttacatt tgcgcctttt ttgctgactc
     6241 tttacaatcc ggacagagtc catacaattc caaccgatgt tcattaatct caaaatcagt
     6301 cagtctgctc gcagccattt ctatatcttc aagccctggg taatgaaaat caacaatctt
     6361 accgcagttt tcacaaatta catgataatg acgctttgaa ctgaaatcaa atcggctaga
     6421 agcatcgccg tacttcattt cttgaacaaa tccaatcgaa gtaaataagc gaaggttatt
     6481 gtaaacagtc gcaacactca tatttggaaa ccgatgttca agtgctttat aaatctcatc
     6541 agccgttgga tgggaatgat gttcaatcaa atattctaaa accgcgtacc gttgaggtgt
     6601 gattcgaatg ttctcttttt ttaacttttc gataacctct tcgactaact tattttccat
     6661 gtaaacaccc cacgttgtta ttctcgtctt atcatactat ttttacattt taaaatccac
     6721 ttaacatgaa aagaaaattc aattttattg tattttaatg aaaaaagacg taaaacagtc
     6781 gcgttcacta ttttacgtct ctattcaaat aatttttctc atcataaatt tttgtgtgtt
     6841 cttttcatct ttaaaagttt tttacataaa cctatctgga gaaaaaccaa gtgccttata
     6901 tctcttggta aagggctgta gataaataac gttcaccatt gtcgggaacc acagctaata
     6961 ccttcttgcc ttttcctaat tcttttgcga cttttagcgc ggcagcaatt gcagcgccag
     7021 atgagatgcc tactaaaatc ccttcttttc gcccaacttc tctagctgtt tccatcgctt
     7081 cgtctccact aatagaaagg actttatcat acacagaagt atccagtgtt ttaggaacaa
     7141 aacctgtacc gattccttgg attttgtgag gtcccgcttc tttcccttct aaaatagcag
     7201 attctgctgg ttctaccccg attaattcga ttttcggata cacacgtttt aattctcggc
     7261 cagcgccagt gatcgttcct cctgtaccga tacctgcaac aaacgcgtcc aagccattta
     7321 ctccaaatgc ttgatgtatc tcaggaccag tcgtcttctc atgcacaaga ggatttgctt
     7381 cattttcaaa ttgtaatggt aaaaagtagc cgttttcttt tgccaaacgt tctgcttctc
     7441 tgattgatcc agaaatacca tccgcaccag gagtcaaaat caactctgcg ccgtaccctt
     7501 tcatcaataa acgtctttcg atactcatcg tctcaggcat cacgatgatt actttatatc
     7561 cttttgcaac tcctaccatc gacaatccga ttcctgtatt tccagaagta ggttcaatga
     7621 tagtatcacc tggtttcagt agaccatcgt gttctgcttt ttctatcata cttaaagcaa
     7681 tacgatcttt tacacttcct cctggattaa aaaactcaag ctttacaaaa acatctgctg
     7741 aatcttctgg tacgatttta tttaatttga cgattggcgt tttgccaatc aattcggtga
     7801 ctgagttata gatagctgtc atcttcaatt cctccttgtc tttgctcttc ttacttatca
     7861 taatacaatc tgttcattta tggcgagtga ttcgtctttg actagcaaaa aaaagacaaa
     7921 gatataccat atatggtagt ttctttgcct ttttatctaa atcaaacgtt tagttcttga
     7981 ccaactaata aagaataact ggacatagac atgccatttt tcgccattag atcatcaaca
     8041 gacatgccaa atttttgtgc aatatcccat agcgtatcac ctgacacgac tgtatatgtc
     8101 tgaccattat ttgtatttac atctgttgtt gaagatgatg acgttgaagt cgtcgtagta
     8161 gtcgttgttg ttgtacttgt ggtagccgaa cctgttgatg gcgtatcaaa acgggttaaa
     8221 ttataagtat taatcaatga gttcaatttg gccccataat ttggatcggt agcataacga
     8281 cctgtcaagt acgctgttgc atcataaaaa cttgttgtat tgcttttcca cacgccagca
     8341 taatgataat cgcccgtgga aaaactggtt gaacgaagaa cattcgcatg gtcttggaag
     8401 gattcccaat aagaagaata ttttcggaat ggttcattca tcgttaccca ttgtccgtta
     8461 catattcttt ggttgccata taaacgacct gtccgttata gcttcctttc acaccgaata
     8521 gattataatt tggcgcacta gctaatccgg aagtgccata tgcactttcc aacaacgctt
     8581 gagcgatcat gactgaagca tacaagtcat tacttgcagc aacttctgac gcagaccagc
     8641 cgatttgatc gataaacgct tgaggatcag caactgtttg agatccttgt tcatctgcat
     8701 gaacatgttg cggtaccgct gtaaccatca tcggcccaac taaaagactc gttc
//
LOCUS       DAZUKC010000096         8657 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000096 DAZUKC010000000
VERSION     DAZUKC010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.098"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(57..350)
                     /locus_tag="ACG80T_002341"
     CDS             complement(57..350)
                     /locus_tag="ACG80T_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFS4841964.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(378..1043)
                     /locus_tag="ACG80T_002342"
     CDS             complement(378..1043)
                     /locus_tag="ACG80T_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFS4841965.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(1183..1815)
                     /locus_tag="ACG80T_002343"
     CDS             complement(1183..1815)
                     /locus_tag="ACG80T_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS4841966.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDWPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(1822..2889)
                     /gene="liaS"
                     /locus_tag="ACG80T_002344"
     CDS             complement(1822..2889)
                     /gene="liaS"
                     /locus_tag="ACG80T_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFS4841967.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEITKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILKLERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWEVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVETKVPLMKEA"
     gene            complement(2886..3617)
                     /gene="liaF"
                     /locus_tag="ACG80T_002345"
     CDS             complement(2886..3617)
                     /gene="liaF"
                     /locus_tag="ACG80T_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFS4841968.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAV
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            complement(3785..4264)
                     /gene="greA"
                     /locus_tag="ACG80T_002346"
     CDS             complement(3785..4264)
                     /gene="greA"
                     /locus_tag="ACG80T_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFS4841969.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(4400..5575)
                     /gene="mltG"
                     /locus_tag="ACG80T_002347"
     CDS             complement(4400..5575)
                     /gene="mltG"
                     /locus_tag="ACG80T_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFS4841970.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            5740..6900
                     /locus_tag="ACG80T_002348"
     CDS             5740..6900
                     /locus_tag="ACG80T_002348"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFS4841971.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KELNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
     gene            complement(6940..7887)
                     /locus_tag="ACG80T_002349"
     CDS             complement(6940..7887)
                     /locus_tag="ACG80T_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFS4841972.1"
                     /translation="MLNYLITGGAGFIGSTLANYLGKENNVVVIDDLSMGKKENLDES
                     KHITFIEGDVADKNLMEKVMKEYHFDYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFAAEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
     gene            complement(7920..>8657)
                     /locus_tag="ACG80T_002350"
     CDS             complement(7920..>8657)
                     /locus_tag="ACG80T_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFS4841973.1"
                     /translation="TYVVSIIIVAISSFIVVLKRNGTKKDLKNYILAIVSSIGSVLIL
                     FALFNATKILFQELLPHLKEINFFNFTERHKELMNNSQHINNTQTPDNTLVRKDVAEN
                     TDISNARITIWKSAWEIFKTSWLIGVSPKNVVSYAQSIIPDGYIAKVGIAIHNAYLNV
                     LTSTGILGIIPFMAFLIKSFVLNVYRIIYNKCIGKDYHFVYCLVILSYALYAALNNEL
                     VYENTVGTFVFWLFLGRVNSKNNSSYF"
BASE COUNT         2615 a         1784 c         1341 g         2917 t
ORIGIN      
        1 aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct ctttttttat
       61 tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac aagtaaatca
      121 tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc atccatttcc
      181 actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa accatcatga
      241 acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc tggcagatca
      301 atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat cccaacaacc
      361 tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac atcgcctgtc
      421 tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc aggagaagcg
      481 ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg ttgcctgaaa
      541 tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat ttctgctaaa
      601 gaatatttat ctgacagctc taagtagtct agaatattct ttgatactaa gttatgtgca
      661 atccgtatcc ccatgtctcg ctcagggtgt acgacacgat ctgctccgat cttctctagt
      721 acacgggcat ggtactcatt ttgggctttt gctaaaatat tcggtacgcc catttccttt
      781 accatcagag taactaagat gctggcttgg atatcttctc caatagcgac aattacatga
      841 tcgaaatttc ttatacctaa tgaacgaagt gtcatctcat cctgtgcatt tgccacaaca
      901 gcatgggtgg ctatattcat atattcattg actcgatctt cattactgtc aatagccagt
      961 acttcttgtc cagcttcgat cagtgtacgg cagatgcttc cgccaaatcg tccaaggcct
     1021 acaattgcaa aattttgttt caccattttc ctcctttagt atcttctttt ctcagtataa
     1081 caaagccttt ccaaaaacaa aagcaagcat cattattgta aaaaagccaa gaaaaataat
     1141 agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca ccaaaccatg
     1201 tttaaacgca tagatagctg cttgagttcg gtcttctacc tctaacttag ctaatatatt
     1261 cgacacatgc gttttgactg ttttcagtgt gataaacaat tcatccgcga tttcctgatt
     1321 acttttccct tcagaaatca gcatcaaaat ttcattttct cgattcgtta attcttcatg
     1381 caatactgga tcatttcgcc gactcatttt ttccatcatt tttgttgtca cttcaggttc
     1441 tagtactcgc tcgccgcgtt gcgtagccct tatagcatca gctatttcat gtgctgtaga
     1501 cgtcttcaac aaatatcctg ctgctcctgc ttcaattgca ggataaactt tttcatcatc
     1561 aataaaactt gtgacgataa tgattttggc ttttggccaa tcttttaaga ttgcttttgt
     1621 tgattcgatg ccatccattt cttccatgac tagatccatt aaaatgacat ccggtcgaag
     1681 tgccatcgcc ttttcatagc cttgacgtcc attttcagct tctccaatca cttcaatgtc
     1741 ttcttggata gataaataag aagagacgcc taagcgtacc atttcatggt catctactaa
     1801 taaaactttt atcatcttta tctatgcctc cttcatcaaa gggaccttgg tctccacgct
     1861 cgtaccttgc cctttaaaac taatgatttt tactgttcca ccaataccag ctactcgttc
     1921 tttgatattg ttcaatccat aacttcccgt tttggtttca ttcatattaa atcctgtccc
     1981 gtcgtcaatg atacgtaata aaagattgtt atctatttta tgcaaataga cttctaattc
     2041 attagcctta gcatgtctta atgtgtttga taacaattct tgaacgattc ggaacaagtg
     2101 atcctcaatg gaactagtta attttacatc ttctacctcc cattttagcg aaatctggat
     2161 tttattctgc aattccttca ataactgctc aatgccttgt tttaaacttt tttcttctaa
     2221 attgactgga cgcagatgaa gaagcagcgc acgcatctca gactgggaag cgttgatgat
     2281 ctctgccacc attttcaact gcttacggaa catttctggt gtctcacttt tttctgcttg
     2341 ctccgtcaat gccgacatca tcatcatcgc tgcaaataat tgctgtgaaa ctgaatcatg
     2401 aagctcacga gctaatcgat gcctttctaa ttttaaaatc tcttctttgg tttgtccatc
     2461 tacatattga ggacggctag tcataatctg taattcactg gatacttcga tcattttttc
     2521 ttttatttta gtaatttcca tatcaatatc tttgatgtac agatcatccg acgcgcttgg
     2581 gatcggctga tgcaaaagtt tggattcata attgccagaa gaaagtgcac gaagcttttc
     2641 ttcgattttc ccgtattttg ccttttgaat aagtgataac aatagaaata cgatcaatcc
     2701 tacacctaat gaaattgcca tcaaatggaa gataagcgga acgtataata aacgcgcacg
     2761 cagcaattct ccccaccaat gactttggtt actggcataa aaataagtga aaagtgaaaa
     2821 tagaataata aggaaagccg caatccccga ataaacagct agcattgctt tggatattct
     2881 tcccatcata ttcgtatcac ctcgacatct ccaaccaaag tattggtaat gatcttcaat
     2941 ctgcgaggat tttcatcata atcactgctg cagattttga tctgttcgtt ttttaaggaa
     3001 aactgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa acgaatagcc
     3061 actcccaggg gtactaagat ccgtgttctg cctatccctt ttcttacaat cacaatatta
     3121 tcatcttttg gcaaaagtgt attgcccaaa tcgatgatag tatcaccaga aatgatggca
     3181 atgttgatat catcccattc atagacgtcg tttccaatac gttggttacc aaataattgc
     3241 tgtcttttac gctcattatt atgtgttttt aattgctctg tttggaccat catgatctgt
     3301 ttttttcgcc aaaaagcatt ttttgtcaaa tctattcctg aaatctctac acctttcagc
     3361 ccgataaata aaacggccaa tacaaccatc atccataaag caggactgtt gactaagcta
     3421 aaaaaaatga ccaagctccc taagatcaat tgaaaatttt gaaatttagt acgaggatat
     3481 ttgcgcacag ctagatatat attgaaaata ccgaatatta ctaaaagcaa aagtccagta
     3541 ttgtttacga tttgccaaac agcaaataac agcagcagtg cttcgactac cacaaaaaaa
     3601 cgccaagaac tattcatcgc tttttctccc tcctgacaca ttctcatttc atcataccta
     3661 tttttttgac tgttggctat aggactttag atgtaagatc gttcagtgaa acctgcaata
     3721 ctcaaagaga aaagatctag agtgcgtcat cagtaagatg aactctctag atctttcagt
     3781 cagactacgc ttgttctact ttgacgattt taactaacat atcgccacct ggtgtagcaa
     3841 tggttacttc atcattttta cgtttgccaa tcaatgcttg cgcgatagga gaatcattgg
     3901 aaatctttcc agaaaacggg tctgcttcag cacttccgac aatcgtgtac tcttcttcat
     3961 ctccatcagg caactcgatg aacgtaactg ttttaccgat cgatacctca tctgcatcta
     4021 cgccgtcatt atcgatgatt tgtgcaaaac ggatcatatt ttcaattgtt gtgattcttc
     4081 cttcaacaaa tgcttgttca tctttagctg attcgtattc ggagttttcg gaaagatctc
     4141 cgaaaccgcg agcgattttg attcgttcaa tgatttctcc gcgtttaact gtttttagtt
     4201 cttctaattc tttttctaat ttttctttac cttctagagt cattggataa actttctcta
     4261 ccatgtgtaa cgtctccttt tttcgataaa ataccgcaaa aggtaatctt ttgaggaata
     4321 tagtccaaaa aagattacca cgcttttctt aaaatgtaaa tgtttttcta aaaatgatca
     4381 atgactagcc cctgtttttc taagaattat ttacatattt ttgcactagt tccatatgtt
     4441 cttcatacgt tttagcgaaa taaacatttc cagtgctcgt atctgctacg aaataataat
     4501 agtcattttc tgctggttct aatactgcct tgattgcctc ttcgcttgga ttgtcaaaag
     4561 gaccaggccc atagccggta ttggtataca aattgtatgg tgaatctaca gctgtatctt
     4621 catacgtcac caattcttta tgttctccta atgcatataa aacagagata tctgactgaa
     4681 gtggcatatt tgccttgatt cggttaaaga agacttgtgc aatattttta cgatcatttt
     4741 cttttacgcc ttctttttca actagagaag ccaaagttaa aacttcttgt acagtcaaat
     4801 ttttctgttt gatagctgaa taatagtttt gcatgaccgt atttgttttg ttcaccatct
     4861 caatcacaag atccttaagc gaagtactcg tgtagtaatc ataagtagct gggaataagt
     4921 agccttccaa acggtatttt gtgttctcag ctttagaggc actttccaac aattctggga
     4981 atttttgata caactcgtta aagaaagtct catcatttac taaatctaaa aattctttct
     5041 tgtctttacc agttaccttc gctacccgct cagcaatctg atcgatgtcg tagccttctg
     5101 gaatggcaat tttcccatct gcaacttttg ttggctcaga tgtgccgcct tcttgcaact
     5161 gcttgccaat ctcatcaaga gtcatattag gcgctaactg ataataacca gcttgaaagc
     5221 cagttaagtt tttaaatttt gtataataat tgaaaacaat cccactttta ataatattgt
     5281 ctttctccaa aatttcgcca atctgcttat tagaagagcc gcttgggatt tccacttgga
     5341 ctaattggtc gtccgattta tctaaaggct tcaacccgct atctacataa cgatatacgg
     5401 taaacccgag aaatccgcca ataatcaata atgtaagagc gatcactaaa atgatcttcc
     5461 tcacgatctt atcttctttt ttacgcagat ttctccgctt gtcgattata tctgctgtag
     5521 ctgccttact ggcttctttt ttgtctttgc ttgggtgttg tttgtttcct gacaaaacaa
     5581 ctcctcctta ctatcatctt cgtaactctg tttattatac atgaaatttc tactgccgtg
     5641 aattatttta ccaaatatta tgtcaaaatt gtaagtggca aatgctcttt ttgaggatta
     5701 caatgaaaaa tagttaaaaa tggataggaa ggatttaata tggcaaaagg aaaacatcaa
     5761 aaaaaacagc agatctctct ttcttttctc gcagttttgt tgctggctgt cagcttcttt
     5821 tcaacaggca tcattacagg atggcaatta acaaaacaac ctcaaccaca aatttataaa
     5881 acggaacccg cacaacttgc ttccaattta acatctaatg agcagatgct tgccagcaaa
     5941 attgatcaat tactgcaaaa atcagattat atcggttcga tttatgtacg caaaaataat
     6001 cgagtgatat tagaaaaagg atatgggtac gcaaatctac gcataaaaga attgaattct
     6061 ccttctcttt actatcaaat cggttcgatc caaaaagcga tgactgcttt acttatccta
     6121 aaacaaattc aatctggcaa gataactttt gacacaaagc tatctgaatt ttatcctcag
     6181 attcctggaa gtcagcaaat ctcaatcaaa aatatgcttt acatgcgctc tggtcttcat
     6241 cgaaccgctt ctccaaaaaa acctatgtca gatagtgaga tcattcagtt tgcccttcat
     6301 catttgaaat ttactggtta tgatacgtat cattatgaac cattaaatta tacattgctt
     6361 acaggggtat tgatcaaact aatgaatcag ccttatgaaa ctcttttaaa aaaagaaatc
     6421 attcggccac tccatctaga acatacagat ttttatgaaa atgtaaaaaa ttccccgcag
     6481 catgctgtat cttaccaaat gtctgcaact gacgattatt caaaagcatt ggtcgagcct
     6541 gaaacagaca tccgaaatga attaggcaca ggaaatataa gtatgtctgt ttatgacttg
     6601 aacaaattct ttaccagtgt tctgtctggc gatattatac caaaagaact acttttctca
     6661 ttgtggcaaa ataatggtga taaacatcct tacagcggcg gtgtttacag tggaaatgat
     6721 tatatccttg ctcagggaaa tatcaatcga ttccatgcag tagcggcgat gaaaaaagac
     6781 acaaaagatg caatcgtcat ggaaagtaat atccaatctg ataaaaacat caaattgccg
     6841 gccactgatt taagaaatca gatttacgaa ctgatggaag gtgttaattt aaaaagctaa
     6901 gtcgagacaa aaaaaccagc aggatgctgg ttttttgttt caatcgattt ctgtctttaa
     6961 ataacgttcc attccgttca atatatcaaa tttgggttga taacctaaag aacggatctt
     7021 tgaaatatcc gctaaagaat cacgaatatc ccctgaacgc tccgcttcat atttaagcgc
     7081 tagctctgtt cctaagattt gatcaattga atgaatcaat tcaagtagcg tagtgctttt
     7141 tcctgtaccg acattgaatt gctgtcctaa agctttttct tccttagcaa caagtaaaag
     7201 tgcttgtata acatcttcta tataaacgaa gtctctagat tggctaccat caccaaataa
     7261 agtaaattct gttttttctc ctgccaattg ttttttgtat cggtccacta gaatcgaaat
     7321 aactccgcta tatggagagt taggattttg atttggacca taaacgttaa aaaatcggac
     7381 agcactagtc ggaacatcat ataaatgaca gtaatccaaa acatattgtt ctgctgcaaa
     7441 cttatcgatg gcatatggtg ttaaaggacg gatgaccgat tcttcttttt ttggcaacgt
     7501 tggttcatcg ccataaactg cagctgatga actaaatacc aatcgtttta aaccagattg
     7561 atattttcgt accaattcaa gcaataaaag cgcactgtca aaatttacac gatgagtttc
     7621 tagaggacgt tctacagaat ctgccacact agctacagca gctagatgga aaatataatc
     7681 gaaatggtat tctttcatga ccttttccat caaatttttg tcagcgacat ccccttcaat
     7741 aaacgtgatg tgcttcgatt catccagatt ctcttttttc cccatcgata aatcatcgat
     7801 cacaactaca ttgttttctt ttcctagata attcgcgagc gtcgagccaa tgaagccggc
     7861 gccaccggta atcaagtaat tcaacatttt ttctcctctt ttcaagataa aatttattac
     7921 taaaaatagc tagaattatt tttgctatta actcttccta aaaatagcca aaaaacaaac
     7981 gttcctacag tgttttcata gactaattca ttgttaagag ctgcgtatag tgcataagat
     8041 aaaataacta aacagtaaac aaaatgataa tctttaccaa tgcatttatt atagatgatt
     8101 cgataaacat tcagcacaaa actctttatt aaaaaagcca tgaaaggaat tattcctaga
     8161 atacctgtcg atgttaatac attcaagtat gcattatgaa tggcaattcc tactttcgca
     8221 atatatccat ctggaataat tgactgtgca taagatacta catttttcgg agagactccg
     8281 ataagccaac ttgttttaaa tatttcccat gcacttttcc atatagtaat tctagcatta
     8341 gaaatatctg tattttcagc tacgtctttc ctaacaagag tattatcagg tgtttgagta
     8401 ttattaatat gttgactatt attcattaat tctttatgac gctctgtaaa attaaaaaaa
     8461 tttatttctt ttaaatgagg taataattct tgaaaaagta tcttagttgc attaaacaaa
     8521 gcgaataaaa tcagaacaga accaatagaa gatactatag caaggatata gttttttaaa
     8581 tctttttttg ttccatttcg ttttaacaca acaataaaag atgatatggc aactattatt
     8641 atacttacca cataggt
//
LOCUS       DAZUKC010000097         8589 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000097 DAZUKC010000000
VERSION     DAZUKC010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.099"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(48..1514)
                     /locus_tag="ACG80T_002351"
     CDS             complement(48..1514)
                     /locus_tag="ACG80T_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFS4841974.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1901..2497
                     /locus_tag="ACG80T_002352"
     CDS             1901..2497
                     /locus_tag="ACG80T_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFS4841975.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2555..3037)
                     /locus_tag="ACG80T_002353"
     CDS             complement(2555..3037)
                     /locus_tag="ACG80T_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841976.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3143..3340)
                     /locus_tag="ACG80T_002354"
     CDS             complement(3143..3340)
                     /locus_tag="ACG80T_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841977.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3635..4393
                     /locus_tag="ACG80T_002355"
     CDS             3635..4393
                     /locus_tag="ACG80T_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFS4841978.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4453..4881
                     /locus_tag="ACG80T_002356"
     CDS             4453..4881
                     /locus_tag="ACG80T_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841979.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4927..4999
                     /locus_tag="ACG80T_002357"
     tRNA            4927..4999
                     /locus_tag="ACG80T_002357"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4960..4962,aa:Gln,seq:ctg)
     gene            complement(5149..5520)
                     /locus_tag="ACG80T_002358"
     CDS             complement(5149..5520)
                     /locus_tag="ACG80T_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS4841980.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5735..5920
                     /locus_tag="ACG80T_002359"
     CDS             5735..5920
                     /locus_tag="ACG80T_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841981.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6003..6191)
                     /locus_tag="ACG80T_002360"
     CDS             complement(6003..6191)
                     /locus_tag="ACG80T_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841982.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6215..6478)
                     /locus_tag="ACG80T_002361"
     CDS             complement(6215..6478)
                     /locus_tag="ACG80T_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841983.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6636..6893
                     /locus_tag="ACG80T_002362"
     CDS             6636..6893
                     /locus_tag="ACG80T_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFS4841984.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6964..7155)
                     /locus_tag="ACG80T_002363"
     CDS             complement(6964..7155)
                     /locus_tag="ACG80T_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841985.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7296..7616)
                     /locus_tag="ACG80T_002364"
     CDS             complement(7296..7616)
                     /locus_tag="ACG80T_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4841986.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7639..8073)
                     /locus_tag="ACG80T_002365"
     CDS             complement(7639..8073)
                     /locus_tag="ACG80T_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS4841987.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            8408..>8589
                     /locus_tag="ACG80T_002366"
     CDS             8408..>8589
                     /locus_tag="ACG80T_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS4841988.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
BASE COUNT         2720 a         1695 c         1362 g         2812 t
ORIGIN      
        1 aacaggaact gctgcaaata agcaattcct gtttttatgt tcattgatta atgaatagag
       61 cccattagtt ttttgattgg ttctttcaca gtaaatagca agataccacc gacgattgct
      121 actgcaccga taataccgaa gtaagcagat tctgttcccg gtgtgtagaa gcgtgcaatt
      181 tgtgcattga ttgcttgtga agaagcatca gctaagaacc aaatagcgat tgtttgtgat
      241 tcaaatgctc gtggtgctaa ttttgttgtc acagacaaac caactggtga aagacacaat
      301 tcagcaataa ccacgatgaa gaaacttccc aacaaccaca atggacttac ttttccactt
      361 gtaccataaa gcatacccgg caacatcaat agcaagtacg ataatcctgc aaataaaagt
      421 ccaagagaga atttcactac tgtagatggt tgacgttcac caagtttcgt ccacatcgtt
      481 acgaatactg gtgtcagaat aaagatgaaa attggattca atgattgata ccagcttgct
      541 tggatcgtta caccaaatag cgtagattgt gtacgttcac tagcaaataa agctaagata
      601 gatgaacctt gttcttgtaa tgaccagaaa atgattgctg caaggaataa tgggatataa
      661 gcccatactc ttggtttttc ttcagcagtt acatcttttg aagtcagcat tttgataaag
      721 taatacaacg gtaagataat ccccaataca ctgattgtat tgacaaaaaa gtcaattgtc
      781 agatgaccag ttagttgtgc cccgccgaat actgcagcga taacaacaag tgcgatgatc
      841 aagttacgga tgaatccttt acgttcttca gttgtcaaag gattcggcgg tgttgtaccc
      901 actccgtcta atgtcttgcg tccttggatg atgtattgta ataatccaaa gaacatccca
      961 atagcagcta aagagaaccc taaatgatag ttatattctt gaccgattgt accaacgatt
     1021 agtggtgcta ataatgcccc taagttgaca cccatgtaaa aaatcgaaaa tcctgcatct
     1081 cttcgcaagt cagtctttga ataaagatgt ccgaccatgc cagaaacatt tggtttcaaa
     1141 aaggcggttc cgataataat caatgccatc gaaacaaaca atgtacttac accaaatggt
     1201 aaagccaaga cgatgtggcc aagcataatg aaaactccac cgaagaatac agtgcgtttt
     1261 gctcctaaca ctcggtcaga tacccatccg ccgacgatac tagacatgta gatcaaagat
     1321 ccgtagatcg acatgatagc taatgctgtt gctcgtggta atccaagtcc cccgttggca
     1381 actgtatcgt acatataata gataagtaat gcacgcatac cgtaataact aaatcgttcc
     1441 cacatctcag tgaagaacaa agttgacaat cccttcggct gcccgaagaa gcccttgtct
     1501 tgctgttttt ccataatcta taccccaatt ctttaaattt tcagaaaata ttatacatct
     1561 ttaccgaaaa atttaactaa aaaacttatt taagaaatat aaactaattt tctataaaag
     1621 caagtaattt ttaatggcaa tatcataaaa aaaagaagat agagatttaa taattatata
     1681 tcagctgttt ttttcaaata agcaaaaatt cctaaaacac aacaatgaaa gcgataacta
     1741 agaaaatcct tacataacga tttttcttat taaaattgtt ttttgaactt cgcttatttt
     1801 ttgtgagcat atctgtttac ttactttttg taaggtgcta tacttcattt cgtaatcaat
     1861 taaaaaaatg caaaacgcaa atcaaaggag aaattttatt atgacaactt ttactgacac
     1921 tttgaaaaac cgccgttcaa tctatcatct aggacgtaat gtttcacttt ctaatgaaga
     1981 attaacaact ttgatcaaag aagcaattaa agaaagccca actgctttca atgctcaatc
     2041 aacacgcgca gttatccttt tcggagatgc acatgaaaaa ctttgggaaa tcactgaaga
     2101 agcacttcgc ccattgactc cagcagaagc tttcccaaat acacaaaaca aattagctgg
     2161 tttcaaaaat ggttatggta cagtattatt cttcaaagat actgacgtgg taaaaggtct
     2221 acaagaacaa tttgaattat atgcagacaa cttcccagat tggtcagaac aatcaaacgg
     2281 aatcgcaaca gctaatactt gggtagcatt agttgataaa ggattgggcg caaacttgca
     2341 acattacaac ccagttattg acgaagcagt tgcaaaagaa tggaatatcc cttcaaactg
     2401 gaaactacgt tcgcaattag tattcggttc accagaaaca ccggctgggg aaaaagaata
     2461 catgaacgat gccgatcgct tccgcgtatt tggataattt tatttgaaat aaaaaacaga
     2521 ggttgtgaca aacatcttgt cgcaacctct gtttttattc tggatcaatc ataatatcaa
     2581 tctctgcctc atcgtttaag cgttgggaca aaaatggaac taattcttct aatgatgcag
     2641 aaaatttcag catcggtcct cgccatccgt ttcggaaaaa attcattttt acgattttag
     2701 aaccttttgg ctgatataat acaattttat tgatctcaga aaaaggaaca ccttttttgt
     2761 caaagctatt caaaacaagt ccttcttctg tgattcctct actgtccaac aaaaaactaa
     2821 taaaaatcat tgctgacaag atcccttttg ccaattcatc tgctgtagga tgactgatga
     2881 gtgtatacca aatgactagt gacactaaga agggtatcac caagtagacc catcgatttc
     2941 gcacagtccg ataaataatt tcttttcgaa ccagccaaag aaataaaaac atcacaacaa
     3001 cgaataaaaa caatagaacc gtaaagaatg ttaccatgac aactcgcctc gctttttctt
     3061 ctccagaaaa taggaaaaga atactgattt accttctcaa agaaattgga cttgcagcac
     3121 gccgcacgtc caattgtttt ctttattcaa atgtatcata ttttgcaaca accatgcttg
     3181 ctttttcatc taataattct tggtcctctg cagcacattg ggcaactttg accacggcgc
     3241 agtttgtcat ttttgataca acttcaccaa tgaccgtttt tgtaaaacca attggtttgc
     3301 atgcatattg ttctcctaaa ttaatcattt cttccgtcat caaaatcctc ctaaactgct
     3361 ggaacttttc tccacattac cactgctatt tctttggaga tttaactcat tgatttttat
     3421 ttacagttaa aaatcaacct tacaggcatt atagcgtact gttttcgttt tcacaattaa
     3481 aacagctgtt gtatttagaa gaattttttt caaaaaatta aaacatattc cttaaaaact
     3541 aattatttcc tacatattaa aatataatac ttccatttcc aataaaaata catttataat
     3601 aggaaagata aacagctgaa aagggagatt tcaaatgaag aaaaaaacgt tcaaaaaact
     3661 attgtttcta gggggcacag ctgctctttt attaagcggt acaagttatt acctacatat
     3721 ccattctcca gaacatacaa tgaacactgc acaaaaaagt tcaacacaaa aaacgaaaaa
     3781 gacaaaagag aaagcagaag ttttactaga tgttccatta gagagccagt ttgattctcc
     3841 ttctttagaa aatggctgtg aagtcacttc gctttctatg cttttatctt tttatggtta
     3901 tgaaacaact aaaaaccagt tagctgagca attggattac gtccctgttt tcaatgcaga
     3961 tgggactcat ggcgatccta atgaaggatt tgttggtgat atcagtggcg gagactgggc
     4021 tatggtcgtc tatgttcccc cagttgcctc ccttgctcag caaatcgtcc aaacggatta
     4081 tcacactttt ccaaaaaccg atgctcaact cgatgatatc aaaaaagcac tatctaaagg
     4141 aaatcctgtc tggacttctg tgactatcaa ttttgaagtc ccagatgaaa ctgattttat
     4201 gacttggacc acaaataatg gagaagtaag agtcactcct cttgttcatg cttgtgtagt
     4261 cactggatat gacaaagaaa atatttatgt caacgatccc tatggtgtaa aaaatcgagc
     4321 agtaccgatc aacgattttt cggcgatttt cactgcaatg ggtggacaga tgctaacttt
     4381 ggagaaaagc tagtaattca aacaaaaatc ccttatactc aagataccaa acctttgaag
     4441 ggagcgtaga taatggaaaa attcacacat aaaaaaatgg atccaaatga aattccgatt
     4501 atttttgtcc gtgactgcaa gggaaatgtt caaggtaaag tctctattaa tgagtggaac
     4561 gagcgtcgca gacctgctac cctcaatgaa ctggaaatca aattgtatcg tcaatctctt
     4621 gtttattacg cagatcaaga gtacgagaaa gccaccgatt tattgaaatt tctaattgct
     4681 cgaacagagt atactcgctt tgaatatatt gaacgtttag caaacatcta tcatattatg
     4741 aatgaacctg tcaaagaata tcagttattg gacaccgtct tgtcagtagc agaactgatt
     4801 gcccttcctg ctggattaga aaagaaacta gtacgccgcc ttttacgagt aaagcaacaa
     4861 ctatccgatc aagaaaaata acttgcgttt ttttaggaaa gtgctatgat tattttgttg
     4921 aataaatggg atgtagccaa actggtaagg cacttgactc tgaatcaagc aattgtaggt
     4981 tcgaaccctg ccatcccaat ttgatgtgct tgctgaaaaa atgaaccccc tgtaaaagaa
     5041 caatcttttt gcagggggat tattgtatta tttttactac ttgtatttag tttacataat
     5101 taaattatga ataataaaat cgggcgtatt cttaatacaa aaactgattt acccaataat
     5161 tgctaatgct ttctccggaa ctttttctct aggaacttcc tcatacgttt ctatgtgtgc
     5221 tcctttattg ctaattttca aataatgatt ttcttttaac acagaaagac cattaaattc
     5281 aaccgttcgt tctttcccat tggcatcagc agctttctgc acataccttg ctgtaccata
     5341 ttcatttact tcctctggtt tttgtgtctt tacatagacc tctccttttt caactaatgg
     5401 atttagctga tcaagtatac cagggatttc tccataactt ccttcagtat aatatttcag
     5461 tccaaatagc aaaataccac taatcgccgc cagtccaatc aaatatttaa tcactttcat
     5521 ctttctacct ccttgttata tattcagtat atgggtcagt taaagaaatt ccatccgtcc
     5581 caagttggag atttttctcc tactttagaa gcgagaatcg agtgtttttt tagtaaaaat
     5641 gatcgttatt tgatattatt gacgtgtgga taaatcagtt tttctcgttt tgtccatgta
     5701 aaaaatcatg ttgatagcaa aggatgtgtt caaaatgaat caagaagaat tatttcaaaa
     5761 agtcaaggaa atgattaaga acggcaattt cgatggcgca aaacgattta tcgaagaaca
     5821 taaagaacaa ttaggacctt ataaagagaa agcccaaaat cttttaaaag atgtaaatat
     5881 cgacagtgtc aaaaacaaat ttaaaaatct attcaaataa gtaaaacttt cacttttgta
     5941 aaatcaaagg aggacaatgc tgctgcatcg tcctcctttg gttcttttct gaatacttca
     6001 tactaaaata aacgtgtcgg atcgtaatga taagtcacat tgccgttcag tgaggctttt
     6061 cccaccacat atttacgatt ctccaacttc agccacgctt ctcgaaaagc cattaattct
     6121 tctcttttta catcaatctt ttcgattttt ccatcgatca aatcttgaat aagttgttca
     6181 tatggattca tttttttcct ccatctgctg tcaatcaata agttgtaaaa ataggttcat
     6241 atgctttttt ggtatctaga tcttctctga ctttgactgt cgtataaata attttttcta
     6301 ctgggatact ataataactt ccttcaccat tattgaatcg gtgaacgtct ttctttgcta
     6361 aaataccatt tattttattc gtcagtacac gttgttcgac actgttactt acaacatagt
     6421 aaatcacccg aatattattg atcaaatgca aatcgatttc taacactaca tgatccatga
     6481 aaatcccact ccttctttca ctttgattat agcaagtttc tcctctaatt tcaaaatcga
     6541 tacagatttt accggataaa tccacaaaac tgcttgcgcc atttcgcaca aagtgttatt
     6601 ttgtatcatg gactttattt atcggaggca atagtatgat cgcgatcggt caatttgttt
     6661 tttatattcc tttttttatt atgataagca ttcttttcta ttacataaaa tggacaaaaa
     6721 agaagttttc agttttactg gcctctttac ctgctgtata ttttacttat caaatttttt
     6781 cttttaggca ttgggaaaca acgtctgtat taataacaca catcatcgaa ttgacgcttt
     6841 cagtaatttt tcttattatt tggatctatt ttttatataa gaatcagaat tgaatccctc
     6901 gcatgtttaa aaagaaaacg attaaagatg cagcaaattt ctgccgatct ttaatcgttt
     6961 tttctattct gttataccaa aaaaatgttt gaatcggtga aggatcgttc cttgttttaa
     7021 ttctataaat tcttgtgcga tcgggtactt ttcttccaaa ttttctttgt tcttttcgat
     7081 caggcttttc gcttcttcat attttttatc tgcacatagt gcttttactt cctgtaatac
     7141 ttttcctgct tccatttgcc tcaccctctt gccattttat tgctttaagt tttatttaag
     7201 ttatacgcat tattctagtt tcatcccaaa taaccttttt tcatttttac tcctcgaact
     7261 aatcagtttt ctaaaggctg attggttcgt cttttttatt tttcctccat ttcattctgc
     7321 tcttctgtta atttatctaa tgctttgatt gatgctgcgg ctcttgcata atcatagctg
     7381 tataattcca taaatttatc aggcagaaca aaccacgctg gatgataacc gttagctgtg
     7441 acagggaatc ctttatcttg ttggttatct ccatcccatc tcagagcaac accttttttt
     7501 cctttccact ttactaaagc gatcgagtat cccttctttg tttgtgtgcc gtcttcaata
     7561 attgctaaaa tttctatttt ttcttttggc gcacggactt cttgaggacg aaccattttc
     7621 tattcctccc ttatctcatt acttcacgac caacacactg caaggggcat gtgatacgac
     7681 ataagaagcg gtcgatccaa ctaatgcttg ttgtatagca ccttttcctg tcgctcccat
     7741 aacgatcaaa tcaatatttt cttgtccagg gatgacattg gcaatcaaat ttcttggatc
     7801 tccagtttca actattgcgt ggatctcagc aatgccgtac tctttggcat cattgatttt
     7861 tttcagcatg gctacttctg ttctttgttt ttcatctgca tagatttttg aaaaggcata
     7921 ggcactatgg gtcagctcaa ctttgttgat gattgacacg ataaataagt gggcttcatt
     7981 gtcctttgct aagtcaaggg cttcagcgaa cgctttgatc gattgtcttg atccgtcaac
     8041 tgctaccatg atgttctggt atcttttact cattggaacc gcctcctttt tcttttattg
     8101 taacatctca agcaaaaata caaaaaatat acgcaatatg taaataaaat gatgaacaaa
     8161 agcaatttct ttcaagttat ttatacattt cttttctcat cagaacacct gttctaatcc
     8221 cattatttct gcattatgtg aaattcctca tcatttttca catcgagcga acatcattct
     8281 gttacaaaaa acgtggattt gtcataaatt gtcatgcttc ttttacctat gaaatatttt
     8341 cacgtgttaa agtattcctc gttgaacaca acaaataaaa ctttttaacg aataaaggag
     8401 atttttcatg acatcactta aaactttact ttttggaaca actttggctg ccggcgctgc
     8461 attcttcatg ggaacgactg ctcatgcgga cgaagcttat accgttcaat caggtgatac
     8521 tctatcaacg atttctcaaa aatacgttgg tgataactcc ttgatcaatg ccattgcaga
     8581 atcaaattc
//
LOCUS       DAZUKC010000098         8426 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000098 DAZUKC010000000
VERSION     DAZUKC010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.100"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            140..289
                     /locus_tag="ACG80T_002367"
     CDS             140..289
                     /locus_tag="ACG80T_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFS4841989.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            300..1814
                     /gene="dltA"
                     /locus_tag="ACG80T_002368"
     CDS             300..1814
                     /gene="dltA"
                     /locus_tag="ACG80T_002368"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFS4841990.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1811..3016
                     /gene="dltB"
                     /locus_tag="ACG80T_002369"
     CDS             1811..3016
                     /gene="dltB"
                     /locus_tag="ACG80T_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFS4841991.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3061..3294
                     /gene="dltC"
                     /locus_tag="ACG80T_002370"
     CDS             3061..3294
                     /gene="dltC"
                     /locus_tag="ACG80T_002370"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFS4841992.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3297..4565
                     /gene="dltD"
                     /locus_tag="ACG80T_002371"
     CDS             3297..4565
                     /gene="dltD"
                     /locus_tag="ACG80T_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFS4841993.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4706..5788
                     /locus_tag="ACG80T_002372"
     CDS             4706..5788
                     /locus_tag="ACG80T_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFS4841994.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5788..6591
                     /locus_tag="ACG80T_002373"
     CDS             5788..6591
                     /locus_tag="ACG80T_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFS4841995.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6661..7401
                     /locus_tag="ACG80T_002374"
     CDS             6661..7401
                     /locus_tag="ACG80T_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS4841996.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7412..8416
                     /locus_tag="ACG80T_002375"
     CDS             7412..8416
                     /locus_tag="ACG80T_002375"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFS4841997.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
BASE COUNT         2706 a         1389 c         1737 g         2594 t
ORIGIN      
        1 cagtgatttg atgtgctatt tttataaaag ctatgtgaca aaactgttag taaaaaaacg
       61 gtttctgtaa gctagacaca tgtttctttt tgtcaaaatt agttacactt tgtgtgaggt
      121 tatatgagga gggaatccaa tgaacaaatg gcgttctaat ccaaaattga attattgggg
      181 aaaattcatc attcgaacaa ttttttatag tgcagtactg ctgttcctga tttatctgta
      241 tcactataaa aatattcaag gaggaacttt tatctataac gaattttagg aggaagagta
      301 tggaaatcaa aacgattatt gaagcaatcg atgattgggg aataaatgaa cctgatcgag
      361 ttgcttatca ggcggatgag acgcatactt ttggtgaatt gaaacaagca tcagacgcac
      421 ttgcttatta tttagaaaaa aaggtagaag gcgatggacc aattgtcgtt tttggcaatt
      481 tggagtttga aatgattgtc tcatttctag gggttgtcaa atctggccat gcctatttgc
      541 cgattgaaga gcatacaccc aaggagcgta tcctatcgat ctttcgggtg gctaaaccat
      601 ctatggtcat cagtatcggg gactggatag aagatattcc ttcggttcct gtcataacaa
      661 aaaaagaatt tcaagaaatc aagaagatac cagtaggatt ttatcccaga aatagcgtaa
      721 aaggtacaga aaatttttat attattttta cttctggtac tactggagaa ccaaaaggcg
      781 tacaaatcag tcatgataat ctagtcagct ttgttcaatg gacgatggaa gactttggga
      841 tcaagcctgg tatgcatttc ttagcccaag cgccattttc ttttgattta tcggtatttt
      901 ctatttatcc tgcattagtt tctggcggaa tgctgaagcc gttaaataaa gcagttgttc
      961 aagactttcg tcaattattc gcgacattgc cagccttgaa gttgaatgtg tgggtttcta
     1021 ctccatcatt tatggatatt tgcttgatgg aacctacatt caatggagaa aatgtgccgg
     1081 aattggacat gtttttattt tgcggagaag aattgacgaa aaaaactgca gaaagtctac
     1141 ttgatcgatt cccaaatgca cggatatata atacttacgg tccaacagaa gcaactgtag
     1201 ctatttcaag tattcagatt gaccagcatg tactagatag ttatgaccga ctaccgattg
     1261 gctacgtaaa aaaagacacg aaagtttcta ttgtccaaga aggtaaacct gtttcaaaag
     1321 gagaaacggg tgaaatcatt attgcaggac caagcgtatc aaaaggttac ctgaacaatc
     1381 ccgaaaagac agctgctgcg ttcttttgtc aaaatgactt agcaagttac catacagggg
     1441 atgcaggtag actagatgaa tatgggcttt tattttatga aggacgtatg gatcagcagg
     1501 tcaaattaca tggataccgg attgaactcg gggatatcga acattatctg ctccaagaca
     1561 accgaatcaa acaagctgtc gtcgtaccaa aataccaagg aacaaaagta cagcagctgg
     1621 ttgcttttgt cgtattagaa aacaaaacga aacagccgga ttttcagttg actaaatcta
     1681 tcaaagaaca attactagaa gttgtaatgg attatatgat tcctcagaag ttccattatg
     1741 tcgatctgct gcctcagaca gttaatggaa aaatcgatcg aaaaaaatta acagctgagg
     1801 tgaatcctga atgatgttac ctcatatgat cccttatgca gagccgtttt atttttttct
     1861 tttagccatt gcgcttgtac cgatcgttct atctttattg atatgggaaa aaagattacc
     1921 agtttatcaa tcagcagtga cacttttctt tttatttgtc agctttggag gtaattattg
     1981 gcaacaggga gcggcgctga ttgggtatgt tttgtggcag acgatacttg tatggggcta
     2041 ttttgtttat cggcaaagga aaaatcagac aggatggttt tttgccgccg ttattttagc
     2101 gataattcca ctgtttttaa cgaaagtgac tccttttttt gaacatggaa aagcatcgtt
     2161 acttggtttt ttaggaatct cttatttaac ctttaaatct gtacaaatga tcatggaaat
     2221 aagagatggc atgatcaaaa catataagcc atttcgttat atccagtttc tgctgttctt
     2281 tccaacaatc tcttctggac caatcgatcg ctataggaga tttgaaaaag atcagctcca
     2341 tccgcctgtt cgtgataaat atgttgatct tttgggaaaa ggcgtccaaa atatttttgt
     2401 cggtttttta tataaattca ttatcggtta ttattttggt caagtactgc ttcctgtagt
     2461 gggacgcatt gccttacagc atggtggtat ttcatgggca ttagtaggtt atatgtacgt
     2521 gtacagtatg tatttgtttt ttgattttgc tggctatagc ttatttgcag tcggtacaag
     2581 ctacatgatg ggatatcaga caccaatcaa tttcaataaa ccttttttat catggaatat
     2641 caaagaattc tggaatcgtt ggcatatgac gctgtccttt tggttccgtg attatgtata
     2701 tatgcgattg atgttcttca tgatgaagaa aaaatttttt aaaagcaaga tcgtaatgtc
     2761 aaatatcggt tattttgctt tgtttcttct aatgggtata tggcatggat tgacgtggta
     2821 ctacatcgta tatggactgt atcatgcttg tctgatctgt ctgacagatg catggatccg
     2881 atttaagaag aagcataaag agcagttgcc ttctaacaaa tttacgcatg gcttagccgt
     2941 atttttgaca tttcaagcag tatgttttag cttcctgatt ttttcaggat ttttagataa
     3001 actttttttt agctaacgaa tagtgacata ttaatttata taacaaagga gaaaaggaaa
     3061 atggaagaac aaattttatc aatcttagaa gaaataacag gaacagatga agtaagaaaa
     3121 aatcgtgata ttgatctatt tgatgaaggg ttgcttgatt ctttagcatc tgttcaatta
     3181 ttagtcgagc tagacggaga gctggatatc caagtccctg tatctgaatt cgagcgagag
     3241 gattggagca caccgaataa aatcattcaa caagcaactg cattgaaggg ttgagtatga
     3301 aaaagaaact ttttgctatt ttcggcccgg ttgttttagc tgttggctta ctgtttgtgt
     3361 tttttacctc ctcctatcaa gtagatatct ctaatccaga aacgattaga cgtgcctctt
     3421 cttccatgtc agtgaacgtg ttgaaaggag atgcgataaa aaattatgca ttatctgaaa
     3481 aacaatatat tccttttttt ggctcaagtg aattaagcag aatcagtcca tttcatccgt
     3541 ctgttctggc agaaaaatac cagcgaaact atcgtccatt tttgctggga gcacccggga
     3601 cacaatcgtt aagccaatat atgatgatgc gttctgcagg agatgctatg aaaaacaaga
     3661 aagtcgtctt tattatttct cctcagtggt tcgttaaaaa tggtgtaaaa actgattact
     3721 ttaatacgta ttattctgaa cttcaaacat acgactggtt gttttcaatg aagaaagtaa
     3781 cgcctgctga tcgctattta gctagaagat tattaacttt ctcaaaagta aaagagaatg
     3841 atacgttgac ggcgattctg caaacgatca aaaaagggaa gctaccttta cctgaatctt
     3901 tgaatcagct gcgcagccaa tggaacatgc tgaagagaga ggatgaagta ttcagcaata
     3961 ttgggttgaa agatcgacag caaaaaatcg atcatgagtc aaaacgatta ccaaaacaat
     4021 accaagaaac agaattatcc attttagcaa atcaaattgg agaacgagaa acgacgaata
     4081 atccattcgg gttgaaaaac gatttttata cgcatcgtat ccgagcccac gagccagagc
     4141 tcaaacagtc acaaaaaaat tgggattatc gtttttcgcc agaattttct gattttcagc
     4201 tggtcttgga tcaattagca aaaaaccata acgaagtatt gttcatcatc ccaccggtta
     4261 acgagaaatg gagcgattat accggactat ctcaagaaat gttacaagga tttgccaaaa
     4321 agatcaaatt tcaattaaat agccaaggat tcaatcgaat agctgatttt gtcaatcaag
     4381 cgggtaccaa ctattttatg gaagacacga tccatctagg ctggaaaggc tggttggctg
     4441 cagatcaaca gatcagaccg tttttagaag aaaatcatat tactgctagt aagtatcatc
     4501 ttgacgatgc tttttttagc aaatcttggc aacatcaaat accagataaa ctacaattaa
     4561 aataaagaaa acaggcgagt gttgctcgct tgttttcttt atttttgaat attctgaata
     4621 aaagataaaa ataccaagtt tgcctgaaca aaaatcataa gcaatggtat aatcacaata
     4681 tgaatataga aatgggggaa aacatttgga tacaaaagaa gaacagctac ttattgattt
     4741 aacttctgca aaaggagtac ctggaaatga agaagaggta agagaagtat tcagagaata
     4801 tgccaaacca ttcgctgatg atatttttta tgacggttta ggcagtgtga ttgccaaaca
     4861 tggtgctggc ggaggcccca aggtctttat ttctggacat atggacgaag ttggttttat
     4921 ggtaacaaaa ataaccgaaa aaggattctt ggaattccaa actcttggag gttggtgggg
     4981 gcaagtcatg cttgctcagc aagttgaaat caaaacacgt gaagggaaaa tcgtccacgg
     5041 tgtgattggt tccaaaccac cacatgtttt aacacctcaa gtaagaaata aaccttatga
     5101 aataaaggat atgttcattg atattggcgc atcaagccaa gaagaagcca aagagtgggg
     5161 catacgtcct ggagatatgg tcactccata tattgaatac aaacgaatga atggcagtaa
     5221 atatttactg gcaaaagcat gggataatcg tatcgggaca gctgtttctc tgcgagtatt
     5281 agagaatcta tctaaagaag cacacccaaa tgttttattc gcaggaagcg atgtgcaaga
     5341 agaagtaggc ttgcgaggtg ctcgaacaag tacacacctt gtaaatcccg atattgcttt
     5401 tgctttagat actggtacag caggagacac tccaggaatg acaccaaaag aagcagattc
     5461 gattttagga aaaggcccac agattttgat ttttgatgca tctatgatcc cgcataaaaa
     5521 attattgaat tttgttattg atgtagcaga ggaaatggat attccattcc aatatacagt
     5581 gatttctggc ggaggaactg atgcaggaca aatgcatttg acaagagatg gtgttccgtc
     5641 tgtagccatt accgttcctg ttcgttatct gcattcccat acatcagtga ttcatgaaga
     5701 tgattatttg aatacagtga aactagtcac agaattagtg aaacggcttg atagtgaaac
     5761 agttgaaaaa attcggagtt atgaataatg aataatagaa tacctatttc tgaatatcgg
     5821 aaaaaagcac gagtttcttt ggaaaatcaa tggggaatca atacttggct tgttttctta
     5881 tctataatat ttacgagcgt catccagaac ttttttgacg gtttcagtcc ttttccggaa
     5941 gaaagcatgc agcagcactt attgagtttt ctagtcgata atttactttt attcgcattc
     6001 aactatgcat tgttttacat tgcgctagta gttgtacggg gtggcagacc aaaaagtaac
     6061 cttttatttt cagttttcca aagaaagtat tttggttcga tacttggcat caatttgatc
     6121 aattcagtca tcaactggct actcaacagt ttaatctttc tgcccatgtt tttaatcagc
     6181 ggagtgagta cttatatgca gctgcttttc agttcaaatg cggtacctac gggtgtcatg
     6241 ccagaagatt ttcttgatat cagctttgtt ttatcgatga tagggatgat ttttgtttca
     6301 gcactcatca tgagtattgt tggcggttta ttccaatttg ctgcatggac aaagttcgac
     6361 tatcctgatt tgtcggttat tcaatgtttg aaatatggct ggtttttatt gaaagataga
     6421 attggtacgt atattttgtt gcagctgtct tttatcggct ggtatttgct aggagcaatc
     6481 gctttgttca tcggtctttt gtgggtcatt gtttatgtga atgtgtcagt tgcagaattt
     6541 tatgaacaag cacggattga aaaaggaggt ccagacgcct attttactta gcatgtaagc
     6601 agtttattca tcaaaaaaat aacttttgtg gtaggataaa tctttgaggg ggaatgtacg
     6661 atgaaattaa ctgttttagg atgcttagga gcgtatcctt ataaaaatca agggacaaca
     6721 ggttatttgc ttcaatctga cgggttcaat ttgttgatcg atgcaggtag tgctacgctg
     6781 atcaaattac aagaacacct tgatccactt gatctagatg ctgtgatcct tagccattat
     6841 catcatgatc atattgctga ccttggtgta ttacagtatt actggcaact tcatcctaag
     6901 agaaatccgg agacattgct ttcaatttat ggacacacaa aagattccat tcactttgaa
     6961 gaattgacgt tggaaaatgt ctcaaaagga catccttatt ttgaagctga agaattaaaa
     7021 attggtcctt ttctgattac ttttatggaa acaatccatc cagtagtatg ttacgccatg
     7081 cgtatcgtag aagaaacaac ggggaaagta ttcgtattta caggtgattc aggttatttg
     7141 gaaagtttcg ctgattttgc gaaagatgct gatttgtttc tagcagatac ctatcttttt
     7201 gaaggaaatg aacatcatca tgcccatttc acttcaaaag aagctggaga acttgcgaaa
     7261 tcagctaacg taaagaaact tgtacttaca cacctacctc agtttggtga tttagaacaa
     7321 ttgaaaaatg aagcgcaaaa ggcagcaggc aaagggattg atatagaact tgctgcagtt
     7381 gacaaagtgt ttaacattta ggaggaagaa catgattttt gtaccaaatg aaaaccacga
     7441 cccaagaatc aatttagcaa ttgaacaata tttgctgcaa gaaatgcctt tagatgaacc
     7501 tattttgctc ttttatatca atgaaccttc aatcatcatc ggcagaaacc aaaatacgct
     7561 tgaagaaatc aatcgagatt atgtggaaga aaatggcatt catgttgtcc gtcgcttaag
     7621 tggaggcgga gctgtctacc atgattttgg aaacctgaac tttagtttta tcatgcctga
     7681 tgatggggat tcattccgtg actttgcgaa agtaacacag ccaatcatcc aagcacttca
     7741 tgaattgggt gttgctgggg cggaattgaa aggccgaaat gatctagtca ttgatggcat
     7801 gaaattttct ggcaacgcga tgtatgctac caacggtcgg atgtttgctc atggaacaat
     7861 tatgttcgac tctgatatca atgaagtagt caatgcatta aaagtaaaaa aagacaaaat
     7921 cgaatcaaaa ggaatcaaat cgatccgttc tcgtgtaacg aatatcaaac catttcttcc
     7981 agaagaaaag caaaaaatga caacagaaga atttcgtcaa gaaatcttgt tgaaaatttt
     8041 cggcgtagag tcagtagaag aagtaaaaac gtacgaattg actgatgaag attggaaaaa
     8101 aatcaatcga atctctgagg aatattatcg aaattgggat tggaattacg ggaagtctcc
     8161 agatttcaat ttctctcgcc aaaaacgttt ttctatcggt tcgattgaag tccatttgaa
     8221 tgtctcagaa ggaatgatca aagaagcaaa aatctttggc gatttcttcg gactaggaga
     8281 tatctctgac gtggaaaaaa aattggttgg acagaaatat gataaagcat ctctatcaaa
     8341 aatagtcagc caaattgatg tgaagaaata ttttggtgcg atagaaccag aagaattgct
     8401 taccttactt tattgaaaat aaaaaa
//
LOCUS       DAZUKC010000099         8228 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000099 DAZUKC010000000
VERSION     DAZUKC010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.101"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            328..1251
                     /gene="truB"
                     /locus_tag="ACG80T_002376"
     CDS             328..1251
                     /gene="truB"
                     /locus_tag="ACG80T_002376"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFS4841998.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1255..2196
                     /gene="ribF"
                     /locus_tag="ACG80T_002377"
     CDS             1255..2196
                     /gene="ribF"
                     /locus_tag="ACG80T_002377"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFS4841999.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2591..3376
                     /locus_tag="ACG80T_002378"
     CDS             2591..3376
                     /locus_tag="ACG80T_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4842000.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3389..4240
                     /locus_tag="ACG80T_002379"
     CDS             3389..4240
                     /locus_tag="ACG80T_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFS4842001.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4364..4816)
                     /locus_tag="ACG80T_002380"
     CDS             complement(4364..4816)
                     /locus_tag="ACG80T_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4842002.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5281..5649)
                     /locus_tag="ACG80T_002381"
     CDS             complement(5281..5649)
                     /locus_tag="ACG80T_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFS4842003.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            5799..6281
                     /locus_tag="ACG80T_002382"
     CDS             5799..6281
                     /locus_tag="ACG80T_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFS4842004.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            6318..7328
                     /locus_tag="ACG80T_002383"
     CDS             6318..7328
                     /locus_tag="ACG80T_002383"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFS4842005.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(7443..8156)
                     /locus_tag="ACG80T_002384"
     CDS             complement(7443..8156)
                     /locus_tag="ACG80T_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842006.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         2784 a         1337 c         1667 g         2440 t
ORIGIN      
        1 ttaagaaact gaaaaaataa ataaaaaaaa acggaaaaaa gactggaaga atggacagtc
       61 ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc
      121 caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaaaacataa
      181 catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
      241 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
      301 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat taactttatg gaaagaacgc
      361 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
      421 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
      481 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
      541 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
      601 gatatgtctt tatctgaaga aaaaatagat gaagtgatgg cttcatttat tggtgagatc
      661 acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat
      721 gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc
      781 cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta
      841 gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt
      901 taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaagat
      961 gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg
     1021 ctgccaatag aaaaaggagt gaaacgattc aaacgagtag atattactga tgaagtatgg
     1081 cagaaagtaa aaaacggtat gagacttaat tatactgtat ttgcgctccc agaaatgccg
     1141 tcagaagaaa ttgctttgtt ttataaaggc aaagttgtca gtatctatca gccaaacccg
     1201 aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat
     1261 atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta
     1321 gtactaggat tttttgacgg cgtgcatatt ggacaccaga aagtcataaa gacaggtatg
     1381 gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatcg
     1441 attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa
     1501 gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct
     1561 tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa
     1621 tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat
     1681 tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa
     1741 ggtgaaaaaa tcagctctac ccgtattcga ggattactag acagcggaca agtaaaagaa
     1801 gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca
     1861 cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac agttcatttg
     1921 cctaaagaag gtgtttatgt atcggaaata aaaattgggg aaatttggta tccagccatg
     1981 ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt agaattgtat
     2041 attttggact ttaatcaaga tatctatggt gaacatgtcg cagtacgctg gaatcaattt
     2101 ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaagaggat
     2161 gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt
     2221 ttatgtataa ctagcaattt caataaaatg ggtgataaat aagcccagga aaggggctgt
     2281 gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa gtagcttctt
     2341 cggaaataag ccgaaatttc acaaaaattt gagaagtaat ttttgtaaat tacttcttat
     2401 ttcttgaagc tggccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag
     2461 caattcatga aaacgattgc taaagcgtat catttgcttt agactatata tcttggtgta
     2521 cactcacttt aagacggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg
     2581 agtgggtact atgtataagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa
     2641 ggggattgga acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa
     2701 ctataactcg gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg
     2761 aaaagcagtc tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga
     2821 tacggcttta gaaaattttg ggacactgga cgtttggatt aataatgctg ggatggaaaa
     2881 tcagcatcca acccatgaac ttagccttga agattgggaa agagtattaa aagtcaattt
     2941 gactggcgtt tttatcggaa cgaaagcggc tttaaattac tttacagaga atgataaaaa
     3001 aggaaatatc atcaatatgt cttctgttca tgaacaaatc ccatggccaa cgtttgctca
     3061 ttacgctgct tcaaaaggcg gagtaaaact attcaccgaa acggtagcaa tggagtacgc
     3121 accaaaggga atcagaatca atagtatagg acctggtgct atccgtacac ctattaatgc
     3181 ggcaaaattt gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg
     3241 cgtaggtgaa ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag
     3301 ttatgttaca ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca
     3361 aggtggaaaa ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt
     3421 tgggtggggg atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat
     3481 attaggaact ggtattggag ccgtgctcgt tggcttactt gttcaattat tcgcttctcc
     3541 agctggtatt tcagtgacta catttttgat tagtttactt tctggtgcat tctgggtcat
     3601 cggacaaaca ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat
     3661 ttcgactgca ttacagttag tcggaacctc attgattggt gtcttcgctt ttggagaatg
     3721 gtctggaagt atgggaaaaa tcattggtgc agcagcaatc attcttcttg tatttggttc
     3781 tgctttaacg gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaatcag
     3841 tctattagca tcaacaagta ttggttattg ggtttacagt gcattgccca aactagttga
     3901 tgcaagcgga ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat
     3961 ttatgtgatt ttccgaaaac caaccgcatt ttctgaagac aagagttgga aagcaacaat
     4021 agttggagcc atcttcagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg
     4081 ggttgctact gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat
     4141 gtacatcctg catgaaaata agacaccaaa agaaaaaaaa ttaacgatta tcggattatt
     4201 gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat ggtaaaatct
     4261 catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa aaatagcgct
     4321 agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat acaagtgaaa
     4381 tatctgtctg catcaataat ttttcattga tccattgact aaaatttacg acatgattga
     4441 agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat tgtaaaagac
     4501 ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct cggatttgat
     4561 tgattgctgt aaaatcacga tactgaaggt tgaaacgcat cgtgattggt gcatttagca
     4621 tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg aactcaaaaa
     4681 gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt tttccttcat
     4741 gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat tcatttactg
     4801 agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact tagttgatat
     4861 ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta aaaatcaagt
     4921 cagaaaattc taacttttct ttctctaaag aaattcgttc tctcctaaag tgatattata
     4981 tttaaatata cctgaaaata atgggcagta ttatgaaaga tcactgatat tttctcgtat
     5041 tttttcctta ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt actaagtgga
     5101 aaattcaaag ccattcaatc agtgattttt ttcagcacaa gaaagactcc ttataaaaat
     5161 agcagatact ttgtcaatta agtgtttaac cagggatgat aaaaaggata ggattgaaca
     5221 aaacctgtat aagctgatga atagtttcct cgccagtaaa aggtttattc gtataaagct
     5281 ttatcgccaa aatttatcat gacctttttt atcttcaata ttaaatgaaa tcaattgttc
     5341 aatcaattcc cagttgatta cacttttcca aggaactttg atgacgttat ccgtatatac
     5401 gtagcctgct tcttggatat ttttttcaag ggcacgaatc gctgcttttt ccggtgcgat
     5461 agaaaaatga tttttagcaa aactaaatgc aataataaag gtaccatggt cagtaaacat
     5521 aggttggttc cattttacag ttgtgtctag ctggggatac gtttctgtta tcttagcaaa
     5581 aaggtctttg atcttttgtt gatgttcttt ttctttgatc tgattgatga tcggctgaaa
     5641 tttttccatt ctaaaacgcc tccattaagt ggtttgattt attttaccag aaaaacaata
     5701 gttatgtata aaaaacgaac aaaacacaag taatgttgta accgctatat ttatatgata
     5761 agatgtctca aataataaaa tagaaaagag acataaaaat gattgacttg gcaaagaaat
     5821 tcttacacga aaatatatat tttgagttgt atcatcatcg agccatttat acaaatgaag
     5881 atgctttggt aataaagaaa gaacaaggat ttacaggtac agagaccaaa agcttattct
     5941 taaaagataa aaaagataat aactacgtat atctgacttt tactacgaaa caaacggatt
     6001 tcaaagcatt agggaagatc gtcggtaaaa ggctttctat tgtttcatca gaacgcatgg
     6061 aagaacaaac gggacaaaag cagggcgctg tttctccgtt tggttatgaa aaacagattc
     6121 cgattattat tgatgaagag ctcttatcaa atgaaaaact tgtttttgca ccaggtagac
     6181 cagatcaaac gatggtcatc caaacggttg acttaaacaa aatcatccag ctacttgaga
     6241 tgccgatatt tttgatgccg gcaaacgaaa aaaacaatta accaagtaaa atagtgcata
     6301 cttggaggtg aagcaacatg ataagttatc cattattaga aagaaatgca acgattgggg
     6361 ttactgcacc atcctcaggc gtatcagagg aacaccattc gttactcgaa tcagctttaa
     6421 agagaatgga agaaaaatct ttccaagtag ttactggaaa gacgctttgg acgcaagata
     6481 aggaaaaatc aggtccagca agaaaacgag cagatgaatt caatcaaatg atgcaagatg
     6541 aaaatatcca aatgatcttt ccgccttggg gaggagaatt attgattgag attctcgatt
     6601 ttgtcgattt tgatgctcta caaccaaaat ggatagtagg ttattcagat attagtacgt
     6661 tgctacttgc tgtcacatta aaaacaggga tagccactgc acatggaacc aaccttattg
     6721 atttaagagg agagtacttg gatgaaacga cagcaatgtg ggaaagtgta ctgaaaacaa
     6781 gagaaggaga atcgattcta cagcaacctt ctgaaaagta tcaaaaagag tggccccatg
     6841 agcattcatc atcttctgtt ttccatctca ctgaaccaac aaattggcaa tcagtcagcg
     6901 gagatgcagt aaggatgaag ggacgattat taggtggctg tattgatacg atcagtcatt
     6961 taatcggtac cccttatgga gatgtccaat cttttaaaag aaagtataat gatggaaagc
     7021 cgatcttgtg gtttctagaa aattgtgagc tatcgtcagt tgatttaaga agatcacttg
     7081 ttcagatgaa actggcagga tggtttgatg gcagctcggg gatcatgttc ggtagaagtg
     7141 cagcagagga tttgttagat tataaaatga aggatgtata tgaagatctg tcaaaggaat
     7201 taaaactacc gatcttgtat gatattgatt gtggtcatgt accgccacaa ctgacattga
     7261 tcaatggtgc ctatgcagaa gtagcggtag aagatagaaa agcgactgtt ttgcaaacgt
     7321 ttatttaaaa aattttttcc tgccttggag tttatcaaag accaacaaat ctaaatagaa
     7381 aagaaaatga aagaagctga cagtattttg ccagcttctt tttgtatgat ctattagaaa
     7441 ttttaattat ccataactat cacattgatc agctgtttca catcatttgg attacctgta
     7501 ggaatagcta ctacagatgc attatcaata atttcttgag ttgtttctct aatgaacgct
     7561 ggataagatg tttgatctgc aggcatttgc caatgtgaag gaactggata actagtgatt
     7621 gtcccgtcat ggttacttga atacactaaa actccctggt aactgtattt cccagtgagc
     7681 ataaccacat ctttcggata gactgcactc cctgcatcat aaggggcgat aggtgttcct
     7741 gcaggataat gaataacatg tagttcgaaa tcacccgatg tatctctaaa agtattacct
     7801 ctaattgcta gccaaacacg tgcgtactca atttgtagat cagaatatcc tgctaacgga
     7861 tctattgact gagaagactg tgaggaagtt gtgtctattg ttgaagaaga aatatttgtc
     7921 tcaactgtac tactagatgt agaagaagta ttgggctctg agactttact gcttgttgaa
     7981 gatgtattcg tttcagtaga agctgatttc tgagtacttg atttttcagc tgaactggtt
     8041 tgtttgattg aacttttgtc tgtggtcgca gatggatcac tattttcttt actaggagac
     8101 gtacatccta caaggaataa agagaccaac aataaaataa aacctaactt tttcatataa
     8161 aatacctcat tattttttta ataccagtat agcataatcc tgaatttttg aatccgtaaa
     8221 accttgtc
//
LOCUS       DAZUKC010000100         8199 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000100 DAZUKC010000000
VERSION     DAZUKC010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.102"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            197..442
                     /locus_tag="ACG80T_002385"
     CDS             197..442
                     /locus_tag="ACG80T_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842007.1"
                     /translation="MSKSKKNITIIGLGLTVYLFTEFVYRHVNLFAEPLFVLYESNSS
                     IVSELANLMIALLFTPALRVITILVCLVLLFFVNKKK"
     gene            complement(626..1375)
                     /locus_tag="ACG80T_002386"
     CDS             complement(626..1375)
                     /locus_tag="ACG80T_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS4842008.1"
                     /translation="MLKLERHQKILDLLNAEQKVIASDLSQRFNVSEDTIRRDLKDLD
                     SQGLLKRVHSGALRVGPPITNFNYRETVASEQKTALAHKALPFLKEESVIIIDGSTTN
                     LALAKEIPQDFRATIITNSPPIAIALAQHPNLEIINIGGTFYKQSMINIGVQAYKQLQ
                     NIRADLYIMGIYNIDLDVGTSVPTMQESDIKREMIRISTEVLGMITNDKFGTISNHIV
                     GPIEDLTYLISEEIPEKVRKEYNKQKIVLID"
     gene            1561..2265
                     /locus_tag="ACG80T_002387"
     CDS             1561..2265
                     /locus_tag="ACG80T_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFS4842009.1"
                     /translation="MEKKIILSPSLMCGDLANLEGSIQELESIGMDSLHIDVIDGSFS
                     PSMPLGIETIKRIREITSMNFDVHIMAKDNDNEFFIGEMLKIGVESITFHYETSLHID
                     RYIQLIKNSGTKVGIALNPATPLNVLQYILPEIDRICLMLINPGFATNKSEKQVSYAL
                     KKITDLCQLVNEKELAVDIQVDGRVSLETIPGLLDAGATNLVLGSTSLFLKGNTLAEN
                     QKLLYQAMKQSVRKNQ"
     gene            2305..2862
                     /gene="hxlB"
                     /locus_tag="ACG80T_002388"
     CDS             2305..2862
                     /gene="hxlB"
                     /locus_tag="ACG80T_002388"
                     /EC_number="5.3.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0043800 - hexulose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HFS4842010.1"
                     /translation="MSYKAAKQNIVREITETLDRISSKDVEQLIKKIEKAERVFFVGV
                     GRVLLALEGMAKRLGHLGIQTVVVGQITEPAITDKDLLIVGSGSGESAFPLTIAKKAK
                     EFGSQVAHIGSNPDSTMKEYSDLFIRIPVQTKLALPEEISSIQPMTSLFEQSVLLLGD
                     SIALVMIEEKAIDMSKLWQYHANLE"
     gene            2870..4009
                     /locus_tag="ACG80T_002389"
     CDS             2870..4009
                     /locus_tag="ACG80T_002389"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFS4842011.1"
                     /translation="MKAVHFGAGNIGRGLIGQILHQNGFDLCFIDTNPELIHQLNQDK
                     GYTIEIIDEMNRSDFIDHVVALNSLSELEEVITATKNADILTTSVGAINLVKIAPIIA
                     QALSKRFEQKHSINIVANENVINASDVLKKEIYSLLSEKEQENFDEYAYFVNTAIDRQ
                     SLGKVLGGKMISVVEPYYEWIMDRQQLDPTTSFDMKNVNLVDDMEPYIERKLYIVNAA
                     HAAVAYLGALKGYKTIQEAIQNMEIFNLVKQFLMENIEYFVQKYHFSREELTSFVEKT
                     LNRHGNQKLSDEITRVGSSPIRKLGPNDRLIAPIVKLDSYHLPYDAGIKVIAAGYHYR
                     NLADPEAEQLRRMIVNDGLKATIKKISRLEGKLLNEVVAAYESQI"
     gene            4021..4452
                     /locus_tag="ACG80T_002390"
     CDS             4021..4452
                     /locus_tag="ACG80T_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4842012.1"
                     /translation="MLKEQLSGNTMFMDAIDSWQEAIRVSARPLIGKGIIEESYVQAM
                     IQNVLDNGNYIILLPLVAMPHARPEFGSKGVGMSFLCLDKPVMFPSEEPVKFFFTFSS
                     DSPKGHLDLMANLGELLSDEKMYQKLFDVKTEKALLDLIKK"
     gene            4466..5911
                     /locus_tag="ACG80T_002391"
     CDS             4466..5911
                     /locus_tag="ACG80T_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFS4842013.1"
                     /translation="MEVANKKKRVSMRAKVQAIGGFLTNMVLPNIGAFIAWGIVTALF
                     IPTGWFPNESLNELVSPTVSYLLPLLLAYTGGRMVGGTRGAVLGTIGTIGLIIGADIP
                     MFMGAMIMGPLGGWMMKKTDDFLDGKVPAGFEMVVNNFSIGIIGFILMCLSYLVVGPI
                     IQSANQFVTSAIEALVSTGFLPILSLINEPAKVLFLNNVIDQGVYYPLGMQQTLEAGR
                     SIYFMVASNPGPGLGLLLAYSLFGKKAAKQTAPGAIIIHFFGGIHEIYFPYVLMKPLT
                     ILSMIAGGMTGIITFELFDAGLVAGPSPGSIFSYLALTPRGNFLGVMAGVVTATLVSF
                     FVTSVILKADKSTDEDRNFELSVQKSKTMKSEGRKLLKEENAREICTEKKEMNKISFA
                     CDAGMGSSAMGATIFKKKLQKAGITDVEIKNYRIEEVPEDSDLVIVHQDLEERTRKAH
                     PNMKIVTLKNYLQDPVINGLAEEIIGLKTED"
     gene            6197..7822
                     /locus_tag="ACG80T_002392"
     CDS             6197..7822
                     /locus_tag="ACG80T_002392"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFS4842014.1"
                     /translation="MKKIYSKEIDCFRGIAVLAVIFYHLPNSLLPGGLQGVTIFFVVS
                     GFLMAKNTIRDEKNRQFSFLHFYYKRIKRIYPFLLFSLVVFIFILGISNIRLIGNIRS
                     ELPSLIFGYNNWWQLNQGVSYFDSYFNASLFKHYWSLSVELQFYLIWPFLFIVIKRMR
                     RKQVFYLIYTLIFVSILFSLFLPSTKAYYHTIAKLFPFLLGVWGYFNRITIGRFFEQN
                     TFSKIWLLLLASLCLILFPIFPYTLNELLISICFALLLASVDDMNIAKKTKIASTKLS
                     FVGKISYELYLVHFPVLIALTNLFKHHHWKYLDIAGLFTVFLCTLLMITCFRKGVQKM
                     RSPKIWTAMALMIIGVSVLVLRAPASRLTADQNQLQSLLKENSSKANAGENLLSQEKK
                     ILFIGDSVMLGAYQELKETFKKEALVDAKESRQITALPEILKEHKDLVSYQQIVIGLG
                     TNGVITDEAIEQTMSLLKDKKVYWVNIKAPTGWQDTINATLATLPNQYSNITMIDWYS
                     ESKNHPEFFYDDETHLNESGRTAYAKYIASVIQ"
BASE COUNT         2777 a         1184 c         1636 g         2602 t
ORIGIN      
        1 tatttaatca catttgattc taaaaaagaa acaaaaataa aagtcatgta tatggtggtc
       61 aaaaagtaaa taatatcagt tatcaaattt cctgtaaatt ttcttgaaac tacagcatat
      121 tattacttca aaacaatcta cattttttat aaaaaaggaa atgaataact gaaaaaatta
      181 ttataggaga gaaaaaatgt caaaatctaa aaaaaatatc accataattg gtttaggctt
      241 aaccgtatat ttatttacag aatttgttta tagacatgtg aatttatttg cagaaccttt
      301 atttgtatta tacgaatcaa attcctcaat cgtcagtgaa cttgctaatt taatgatagc
      361 attgctgttt actcccgcgt tgagagtcat aactattttg gtctgtttag tgctattgtt
      421 ttttgttaat aaaaagaaat aacgtagtat ttcaaaagac aaagaagtgg gatgatgatt
      481 gctatagtat gttatacgtg gtgctaggta agtgagaata aacaaaaaag gaaacacgca
      541 ataaagttta tttcaaaact ttattgcgtg tttctttttt agatttttta tatctgttgc
      601 tttagtagtt accaatagtt ttcgcttaat caatcaaaac gattttttgt ttattgtatt
      661 ctttacgaac cttttcagga atttcttctg aaataagata ggtaaggtct tcaattggac
      721 cgacgatatg attactgatg gtgccgaatt tatcattagt tatcatccca agtacttcag
      781 tagaaatacg tatcatctca cgtttaatgt cggattcttg catagttggc acgctcgttc
      841 caacatctag atctatgtta taaataccca taatataaag gtcggctcga atgttttgta
      901 gttgcttata agcttgtacc ccaatattga tcatagattg tttataaaaa gtaccgccaa
      961 tattgataat ttccaaatta ggatgctgtg ctaatgctat ggcaatcggc gggctattgg
     1021 taatgattgt tgcacgaaaa tcttgtggga tttcttttgc taaagccaag ttagttgtac
     1081 ttccatcaat gatgatgaca gattcttctt ttaaaaaagg gagggcttta tgagctaatg
     1141 cagttttttg ttcgctagct acagtttctc ggtagttaaa attggtaatt ggcggaccaa
     1201 cacgtaaagc gccgctgtgt actcttttta agagaccttg gctatccaaa tctttcaaat
     1261 ctcgtcgaat cgtgtcttcg gatacattga aacgttgact taaatcacta gcgataactt
     1321 tttgttctgc gtttaataaa tctagaattt tttgatgacg ttctaatttc aacatgttgc
     1381 gtgctccttc ttatttattc ttgtttatca tgtcatttat gcgtttttat gtcaataatt
     1441 tccgttttta atacactaaa aaggcataaa caagcaaaaa caaaatattt ttatgcagtt
     1501 ttatgttgac tagtatttgt taagcggtta caatgagggg gtaattaaag gagggaatgt
     1561 atggagaaaa aaattatttt gtcgccttca cttatgtgtg gggatctagc aaatttggaa
     1621 ggaagcatcc aagaactaga aagtattggg atggattcct tgcatattga cgtgattgac
     1681 ggttctttta gtcccagtat gcctttaggg atcgaaacaa tcaaaagaat tagagaaatc
     1741 acttcaatga attttgatgt acacatcatg gctaaggata acgataacga attttttatt
     1801 ggagaaatgt tgaaaattgg cgtagagagc attacttttc attatgaaac aagcctccat
     1861 attgatcgat atatccagct catcaagaat tctggaacaa aagtagggat agctttgaat
     1921 ccagcaactc cgttaaatgt attacaatat attttacccg agattgatag gatttgtttg
     1981 atgctgatta atccaggttt tgcaacaaac aagtcagaaa aacaagtttc ttacgcgcta
     2041 aaaaaaatta cagatctttg ccagttagta aatgagaaag agttagcagt agatatccaa
     2101 gtggatggac gggtatcgtt agaaacgatc ccgggattat tagatgcagg cgcaacaaat
     2161 ctggtattag gaagtacaag tttgtttctt aaaggaaata cattagcaga aaatcaaaaa
     2221 ctactttatc aagcgatgaa gcaatcagtt aggaaaaatc aatagatgta gtggtcctga
     2281 ataatagaaa tggaggggaa atagttgagt tataaggcgg cgaaacaaaa tattgtaaga
     2341 gaaattactg aaacattaga ccgtatttct tcaaaagatg tggaacaatt gataaagaaa
     2401 attgaaaaag cggaacgagt ttttttcgtg ggggttggca gagtactgtt agcgctagaa
     2461 ggaatggcaa aacgcttagg gcatctagga attcagacag tagtcgtagg acaaatcact
     2521 gaaccggcaa ttacagataa agatttattg attgttggtt ctggtagtgg ggaaagtgct
     2581 ttcccactaa ctattgcaaa aaaagcaaag gaatttgggt cccaagttgc ccatatagga
     2641 tcaaacccag acagcacaat gaaagaatat tcagatcttt ttattcgtat acccgtacaa
     2701 acaaaattag cgttaccaga ggaaatttct tctatacagc caatgactag cctgtttgaa
     2761 caaagtgttc ttttgttagg agatagtatt gctttagtga tgatagaaga aaaagcaatt
     2821 gatatgtcaa agttgtggca ataccatgct aacttagagt aggtgaaaaa tgaaagcggt
     2881 acattttgga gcagggaaca tcggcagagg gcttattgga caaattctac atcagaatgg
     2941 attcgatcta tgttttattg atacgaatcc tgaactgatc catcaattaa atcaagataa
     3001 gggttatact atcgaaatta ttgatgaaat gaacaggagt gatttcattg atcatgttgt
     3061 ggcactgaat agtttatccg agctagaaga ggtgattaca gcaacaaaaa atgcagatat
     3121 cttaacaaca tctgttggcg caataaattt agtaaaaatt gcaccgatca ttgcgcaagc
     3181 cttaagtaag cgatttgaac aaaagcatag catcaatatc gtagctaatg aaaatgtgat
     3241 aaatgcaagt gatgtgttga aaaaagaaat ttattctttg ctgtcagaaa aagaacaaga
     3301 gaattttgat gaatatgcat attttgtgaa tacggcaatc gatcgacaat ctttaggaaa
     3361 agttcttgga ggaaaaatga tctctgttgt cgaaccttat tatgaatgga taatggatcg
     3421 acagcagtta gatccaacta catcttttga tatgaaaaat gtaaatttgg ttgatgatat
     3481 ggagccttac attgagcgaa agctttatat cgttaatgct gctcatgcgg cagtagctta
     3541 tttaggcgca ctgaaaggat ataagacgat tcaagaagca atccaaaata tggagatttt
     3601 caatctagtc aagcaatttt taatggagaa tatagagtac tttgtacaaa aatatcattt
     3661 ttctagagaa gaattaacat cttttgtgga aaaaacatta aaccgtcatg ggaatcagaa
     3721 actctccgat gaaattactc gtgtaggaag ttcccctatt cgaaaattag gccctaatga
     3781 tcgtttaatt gctcccatag taaaactaga ttcatatcat ttaccttacg atgcaggaat
     3841 aaaagtaatt gctgcgggat atcattatcg gaatcttgct gatccagaag ctgaacaact
     3901 acgtcgaatg attgtaaatg atgggttgaa agcaacgatt aaaaagattt ctcgtttgga
     3961 aggtaagctg ctgaatgaag ttgtcgctgc ttatgagtcc cagatttaga ggaggagaat
     4021 atgctaaaag aacaattgtc aggaaataca atgtttatgg atgcgatcga ttcatggcaa
     4081 gaggcaatcc gagtaagtgc gcgtcctttg ataggaaagg gaataataga ggaaagctat
     4141 gtacaagcga tgatacaaaa tgtgttggat aacggaaatt atattatatt actgccacta
     4201 gtagcaatgc ctcatgcgcg tccagaattt gggagcaaag gggtcgggat gagcttttta
     4261 tgtttagata agccagtcat gttcccaagt gaagaaccag taaaattctt ctttaccttt
     4321 tcttctgatt cgccaaaggg acatttagac ttaatggcca atcttggaga attgctgagt
     4381 gatgaaaaaa tgtatcaaaa attattcgat gtgaagacag aaaaagcatt gttagatttg
     4441 ataaagaagt aaaggagaga agaaaatgga ggtagcaaac aaaaaaaaac gagtctccat
     4501 gcgagctaaa gttcaagcga ttggcggttt cttaacaaat atggtattgc caaatatagg
     4561 cgcatttatt gcatggggaa ttgttactgc attattcatt ccaactgggt ggttcccaaa
     4621 tgaatctctg aatgaactcg taagtccgac agtcagctat ctattgccgt tactacttgc
     4681 ctatactggt ggtcggatgg ttggtggtac acgtggagct gttttaggta caatcggaac
     4741 aataggtttg attattggtg ctgacatacc aatgttcatg ggagcaatga ttatggggcc
     4801 acttggtggg tggatgatga aaaaaacaga tgatttttta gatgggaaag ttcctgctgg
     4861 ttttgaaatg gtcgtcaata atttttctat aggaattatt ggtttcatac tgatgtgcct
     4921 ttcttatctg gtagttggac caataatcca atcagctaat caatttgtaa cgagtgcaat
     4981 tgaagcatta gtttcaactg gttttttacc gatactgtcc ttaatcaacg aaccggcaaa
     5041 agtcttattt ttaaataatg ttattgacca aggtgtttat tatccattag gtatgcaaca
     5101 aacattagag gcaggcagat caatttattt catggtagca tcaaatcctg gccctggctt
     5161 aggactattg ttagcttatt ctttatttgg taaaaaggca gcaaaacaga cagcaccagg
     5221 tgcaatcatc atccattttt tcggcggaat acatgagatt tattttccat atgtattaat
     5281 gaaaccactc actatattat ctatgattgc tggtggaatg acaggaatca tcacttttga
     5341 attatttgat gcgggcctgg tagcaggtcc aagtccaggt tctatttttt cttatttagc
     5401 tctaacgcct cggggaaatt ttttgggtgt catggcggga gtggtcacag caacactggt
     5461 ttcctttttt gtaacatcag taattttaaa agcagataaa tcaacagatg aagacagaaa
     5521 ttttgaacta tcagttcaaa aatcgaaaac gatgaaatca gaaggaagaa aattattaaa
     5581 agaagaaaat gctagagaaa tatgtaccga gaaaaaagag atgaataaaa tttcctttgc
     5641 ttgtgatgca ggaatgggaa gcagcgcaat gggcgcaaca atattcaaga aaaaattgca
     5701 aaaagcaggt attactgatg ttgagataaa aaattatcga attgaagagg taccagaaga
     5761 tagtgatttg gtgatcgtac atcaagattt ggaggaacgg actagaaaag cacatcctaa
     5821 catgaaaatt gtgacattaa agaattattt gcaagatcca gtaatcaatg gattagcaga
     5881 agaaatcatt ggattaaaaa cagaagacta atttatggga atttttgatt aacctttatt
     5941 aaaattattt tgcctaatat aaaaaggttg tgacatggct tgtgtcgcaa cctttttttg
     6001 tcgcacaaat aattaatcgg atattttatg ttcatatttt ttgtgatcaa cgagaactga
     6061 tggggcaaag atacatttta gatactttta actgttaatt tacaaaacaa ggtgaaacga
     6121 atagaaaaat gaaaaaatcg atacggaaga atatgtttga ttaaaacatg catgaagaga
     6181 aggaggttga tgatacatga aaaaaatata ttcaaaggaa atcgattgtt ttagaggcat
     6241 tgctgtttta gcagtgatat tttatcattt gccaaatagt ttgctgccag gcggattgca
     6301 aggagtaacg atcttttttg tggtttctgg ttttttaatg gctaaaaata cgataagaga
     6361 tgaaaaaaac agacaattca gttttttaca tttttactat aagcgtatca aacgtattta
     6421 tccattttta ctgttttctt tggtcgtatt cattttcata ctagggatta gcaacattcg
     6481 attgatagga aacattcgta gtgagctacc cagtttgatt tttggctata acaattggtg
     6541 gcagctgaat caaggagtat cttattttga ctcttacttt aatgcttctt tatttaaaca
     6601 ttattggtcg ctttcagttg aactacaatt ctatttgata tggccatttt tgtttattgt
     6661 cattaagcgt atgagaagaa aacaagtgtt ttacttaatc tatacattga tttttgtttc
     6721 tattcttttt agtttgtttt taccttcaac taaggcatat tatcatacga tagctaaact
     6781 ttttccattt ttattaggag tttgggggta ttttaatcga ataactattg gccgtttctt
     6841 tgaacaaaac acttttagta aaatctggct tttattacta gctagtcttt gtctgattct
     6901 gttcccgatt tttccttata cactaaatga actactgatc agtatttgtt ttgctctatt
     6961 gttagccagt gtcgatgata tgaacattgc aaaaaagacc aaaatcgcta gtacaaaact
     7021 ttcttttgta ggaaaaatca gttatgaact ttatttggtg catttcccag tattgattgc
     7081 tctgacaaat ctattcaaac atcaccattg gaaatatttg gatatcgcag gtctattcac
     7141 tgtgttttta tgtacattac tcatgattac ttgctttaga aaaggagtgc aaaagatgag
     7201 atcacccaaa atttggacag ctatggcact aatgataatc ggagtttctg tactggtatt
     7261 acgtgctcct gccagtcgac tgaccgctga ccaaaatcag ttgcaaagtt tattaaaaga
     7321 aaactctagt aaagcaaatg caggagaaaa tctgttgtca caagagaaga aaatattatt
     7381 tatcggtgat tctgttatgc tgggggcgta tcaggaacta aaggagacat ttaaaaaaga
     7441 agcattggta gatgcaaaag aatcaagaca aatcactgcg ttaccagaaa tcttaaagga
     7501 gcataaagat ttagtttctt accaacaaat agtgatcggg ttaggaacca atggtgtgat
     7561 aacagatgaa gctatagaac aaacaatgtc tttgctgaaa gataaaaaag tatactgggt
     7621 aaatataaaa gcaccaactg gctggcaaga tacgataaat gccacattag ctacattgcc
     7681 aaatcagtat tctaatataa cgatgatcga ttggtatagt gagagcaaaa atcatcctga
     7741 atttttttat gatgatgaaa cccatttgaa tgaaagtgga cgtacagctt atgcaaagta
     7801 tattgcttca gttattcaat aaggtagtga ttacgtgtgt taaccttact ttttcctaat
     7861 aggtgaatca atttaaataa aaaatacttc atatgataaa cggtgtttaa gaggaggagc
     7921 tttttaatat tcgtggtaaa aaagagatgg gcgaggtcgt taactgagag cttcttggaa
     7981 agaaatcgct ttggatttat cctctttgtt atacagaaca tgagcgagac gacaatacta
     8041 ggatgtatgt tctgatcgaa gttttatggt tacaagcagc aatcagctta gataaaatta
     8101 aggatcgtag gaaccaagta aagaaggctg ttagtaaaga aacaaacgat caagtatgtt
     8161 ttactcttaa gaagatgcag tgaataaata atagtaata
//
LOCUS       DAZUKC010000101         8167 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000101 DAZUKC010000000
VERSION     DAZUKC010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.103"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            476..1339
                     /locus_tag="ACG80T_002393"
     CDS             476..1339
                     /locus_tag="ACG80T_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS4842015.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="ACG80T_002394"
     CDS             complement(1468..3090)
                     /locus_tag="ACG80T_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS4842016.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="ACG80T_002395"
     CDS             3339..3860
                     /locus_tag="ACG80T_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFS4842017.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG80T_002396"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG80T_002396"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFS4842018.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="ACG80T_002397"
     CDS             5941..6759
                     /locus_tag="ACG80T_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFS4842019.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="ACG80T_002398"
     CDS             complement(6862..7059)
                     /locus_tag="ACG80T_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842020.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="ACG80T_002399"
     CDS             7228..7806
                     /locus_tag="ACG80T_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFS4842021.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="ACG80T_002400"
     CDS             7901..8038
                     /locus_tag="ACG80T_002400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842022.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2444 a         1475 c         1612 g         2636 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatag
//
LOCUS       DAZUKC010000102         8126 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000102 DAZUKC010000000
VERSION     DAZUKC010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.104"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            70..153
                     /locus_tag="ACG80T_002401"
     CDS             70..153
                     /locus_tag="ACG80T_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFS4842023.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            278..1015
                     /gene="erm(B)"
                     /locus_tag="ACG80T_002402"
     CDS             278..1015
                     /gene="erm(B)"
                     /locus_tag="ACG80T_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFS4842024.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1185..1445
                     /locus_tag="ACG80T_002403"
                     /pseudo
     CDS             1185..1445
                     /locus_tag="ACG80T_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            1608..2273
                     /locus_tag="ACG80T_002404"
                     /pseudo
     CDS             1608..2273
                     /locus_tag="ACG80T_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2309..2554
                     /locus_tag="ACG80T_002405"
     CDS             2309..2554
                     /locus_tag="ACG80T_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS4842025.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2657..3526
                     /locus_tag="ACG80T_002406"
     CDS             2657..3526
                     /locus_tag="ACG80T_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFS4842026.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLLECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3507..4241
                     /locus_tag="ACG80T_002407"
     CDS             3507..4241
                     /locus_tag="ACG80T_002407"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4842027.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4274..5182
                     /locus_tag="ACG80T_002408"
     CDS             4274..5182
                     /locus_tag="ACG80T_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFS4842028.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            5179..5721
                     /gene="sat4"
                     /locus_tag="ACG80T_002409"
     CDS             5179..5721
                     /gene="sat4"
                     /locus_tag="ACG80T_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFS4842029.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5814..6608
                     /locus_tag="ACG80T_002410"
                     /note="aph(3')-IIIa"
     CDS             5814..6608
                     /locus_tag="ACG80T_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFS4842030.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            6884..7015
                     /locus_tag="ACG80T_002411"
                     /pseudo
     CDS             6884..7015
                     /locus_tag="ACG80T_002411"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            7091..7836
                     /locus_tag="ACG80T_002412"
                     /pseudo
     CDS             7091..7836
                     /locus_tag="ACG80T_002412"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7947..>8126
                     /locus_tag="ACG80T_002413"
     CDS             7947..>8126
                     /locus_tag="ACG80T_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibD"
                     /protein_id="HFS4842031.1"
                     /translation="MKPKIIMHTQISLDGRIKGFDNPEVYYQVAGGIHSDAVLFGSNT
                     VFTAFEKYPAETEADF"
BASE COUNT         2834 a         1239 c         1706 g         2347 t
ORIGIN      
        1 tttgtgtata ataggaattg aagttaaatt agatgctaaa aatttgtaat taagaaggag
       61 ggattcgtca tgttggtatt ccaaatgcgt aatgtagata aaacatctac tgttttgaaa
      121 cagactaaaa acagtgatta cgcagataaa taaatacgtt agattaattc ctaccagtga
      181 ctaatcttat gactttttaa acagataact aaaattacaa acaaatcgtt taacttctgt
      241 atttatttac agatgtaatc acttcaggag tgattacatg aacaaaaata taaaatattc
      301 tcaaaacttt ttaacgagtg aaaaagtact caaccaaata ataaaacaat tgaatttaaa
      361 agaaaccgat accgtttacg aaattggaac aggtaaaggg catttaacga cgaaactggc
      421 taaaataagt aaacaggtaa cgtctattga attagacagt catctattca acttatcgtc
      481 agaaaaatta aaactgaaca ctcgtgtcac tttaattcac caagatattc tacagtttca
      541 attccctaac aaacagaggt ataaaattgt tgggagtatt ccttaccatt taagcacaca
      601 aattattaaa aaagtggttt ttgaaagcca tgcgtctgac atctatctga ttgttgaaga
      661 aggattctac aagcgtacct tggatattca ccgaacacta gggttgctct tgcacactca
      721 agtctcgatt cagcaattgc ttaagctgcc agcggaatgc tttcatccta aaccaaaagt
      781 aaacagtgtc ttaataaaac ttacccgcca taccacagat gttccagata aatattggaa
      841 gctatatacg tactttgttt caaaatgggt caatcgagaa tatcgtcaac tgtttactaa
      901 aaatcagttt catcaagcaa tgaaacacgc caaagtaaac aatttaagta ccgttactta
      961 tgagcaagta ttgtctattt ttaatagtta tctattattt aacgggagga aataattcta
     1021 tgagtcgctt ttttaaattt ggaaagttac acgttactaa agggaatgga gataaattat
     1081 tagatatact actgacagct tccaagaagc taaagaggtc cctagcgcct acggggaatt
     1141 tgtatcgata aggggtacaa attcccacta agcgctcggg accccttgta ggaaaatgtc
     1201 ctaagtgtgg caacaatatt gtattaaaaa aatcgtttta tggttgttca aattatcctg
     1261 aatgtaagtt tactttagct gaacatttta gaaagaaaaa actaaccaaa acgaatgtaa
     1321 aagaattact ggagggaaaa gaaaccctgg taaaaggaat caaaaacaaa gagaaaaagc
     1381 cctacaatgc cgttgtaaaa attggggaaa agggatatat tgattttatc tctttttcaa
     1441 aataaacatg gggctgaccc aaaagtagct cctatatgaa aaatagaggt ctttccattt
     1501 agatagtagt ggaaagacct ctatttatct tttgaaagtg aatacaaaaa taaagcacac
     1561 tctgctataa tataggtgat taatccattt gagagaggtg ctttatcatg tatcaaaatt
     1621 ataccataaa ccaactttgt ttgcctatag atcttgaaat taaattagaa gaaaatgatt
     1681 ttgctcatgc cattgtgcag tttgttgact ctattcccga tgaagtcttt cttccttatt
     1741 atcaatctat gggtagaccc caatatcatc ctcgtatgct actttccatt attctttgtg
     1801 cctatattca aggggtttat tcgggccgcc aaatacaaaa tatgctgata gatagtattc
     1861 ggatgcgcta cttatctcaa gagcaattcc ctaactttcg tactattaat cgttttcgcg
     1921 ttcatccgat tatgaacgat attctagatc attcatttgt tcaatttcgt gagctactgg
     1981 ttcaaagtgg cttaatcagt ggcagtgctc tatacattga tggtacaaaa attgaagcgg
     2041 atgcgaataa atactctttc gtttggaaaa aaagtatttt aaagtataag gatagccttg
     2101 atcaaaaagc ccttgaaaac taccaacaaa tggtggaaac aaaaatctta cctgagttaa
     2161 tagatgattt aaaggatgaa ctttctatag aagagattga aaggattcgt caatctttgg
     2221 aagaaaagga aaatcaatta attgaagcga ttgaccaaac cgaaacagta gaagggggct
     2281 gacccattat taataaggag tggacaacat gattgatgta aatgagctat tggaagaagc
     2341 tattagagaa actgaaaatc taaatgatgg cgaagttttt cttgttaaag atttattcaa
     2401 gggatatgtg gggaatagga tacccagaaa ggatcgactt ctgcttggaa cgttattttt
     2461 aaaccatgtt agcaaaatga atggtaattt aaaggctatt gaaaagactt catctaatca
     2521 gcagaggtat aaaaagacaa ttgataagtt ttaactatct tttgtgaaat taaaaaaatt
     2581 ataaatatgt tgagtagaag caaaagattt ggcttggaat ttttacagca gtgaatcgat
     2641 aaaaggagca tgcttgatgg tagataatat tattaaatca gtagcagaga aattatcctc
     2701 tctgtcttat atagaaggta ttgttttagg tggttcacgt gcaaggggca cccatacaga
     2761 ggattcggat atagatatcg gcatctatta caattcagaa tcatttgaca taaatactat
     2821 taatcaattc gctacaaagc tggatgatga gcatagaaat aaccttgttg tacctcccgg
     2881 agcatggggt gattggatta atggcggcgg atggttagtc ataaacgggt atcatgtgga
     2941 tttaatttta cgtgatatta aacgtgtgga acaaataatg aaagatacag agcacggaat
     3001 tgttactgcc aattatcaga ctgggcatcc ccatggttat attagtgcaa tgtatcgagg
     3061 agaattagcg attagcaaaa tactatatgc taagaatgaa agcttatgcg aattaaaaaa
     3121 acaggcagaa acttatccca atgctttgca gaaaagttta gttaactttt ttatgtttga
     3181 agcagggttc tctttaatgt ttgtaaaagc aaattcggga acagacgata aatattatat
     3241 tgcgggtcat gtttttcgta tagtttcatg tttaaatcaa gtgttatttg catgtaataa
     3301 tgcttattgt atcaacgaaa agaaagctat aaaactgctt gaaacttttg aacataaacc
     3361 tgaaaaatat accgagaagg taaatcatat ttttgaagta ctcggtatct cacttttgga
     3421 atgctacgac atgaccgaga agctttataa tgaagtgaat gaaattgtat cggagataaa
     3481 taacttttta aacgaggaga gttcagatga aagaaaacaa atatgatgat aatatatttt
     3541 ttcaaaaata cagtcaaatg agtcgctcgc agaaaggact ggctggtgcg ggagaatggg
     3601 agactttgaa aaagatgcta cctgatttta agggtaagcg tgtgcttgat ttaggatgcg
     3661 gctatggatg gcactgtata tatgcgatgg aaaacggtgc ttcctctgta gtaggtgttg
     3721 atatttctca taaaatgctc gaagtagcaa aaggaaaaac ccattttcca cagattgaat
     3781 atgaatgctg tgccatagaa gatgtggatt tcccagagga gagctttgat gtaatactaa
     3841 gttcgcttgc gtttcattat gtagcagact atgagaattt aataaaaaag atatatagga
     3901 tgctgaaggc tggtggcaat ttagttttta cagttgaaca tcctgttttt actgctcatg
     3961 gaacacaaga ctggtattat aacgaaaaag gagaaatact gcatttcccg gtggacaatt
     4021 attattatga gggcaaacgg acagctatgt ttttggaaga aaaggttaca aaatatcata
     4081 gaacactgac cacatatcta aatacactgc tttcaaatag ttttataata aatcagattg
     4141 tggagccaca gccgccagag aacatgatgg atattccggg gatggcggat gaaatgcgac
     4201 gcccaatgat gctgattgta tcggcaaaaa agaagatgta ataatataga aaaaataaac
     4261 gaggagtatg taaatgagat cagaaaaaga aatgatggat ttagtacttt ctttagcaga
     4321 acaggatgaa cgtattcgaa ttgtgaccct tgaggggtca cgcgcaaata ttaatatacc
     4381 taaagatgaa tttcaggatt atgatattac atattttgta agtgatatag aaccgtttat
     4441 atctaatgat gactggctta atcaatttgg gaatataata atgatgcaaa agccggagga
     4501 tatggaatta ttcccacctg aagaaaaggg attttcctat cttatgctat ttgatgatta
     4561 caataaaatt gatcttacct tattgccctt ggaagagtta gataattacc taaagggcga
     4621 taaattaata aaggttctaa ttgataaaga ttgtagaatt aaaagggaca tagttccgac
     4681 tgatatagat tatcatgtaa gaaagccaag cgcaagggag tatgatgatt gctgcaatga
     4741 attttggaat gtaacacctt atgttattaa aggattgtgc cgtaaggaaa ttttatttgc
     4801 tattgatcat tttaatcaga ttgttcgcca tgagctgctg agaatgatat catggaaggt
     4861 cggcatcgaa acaggcttta aattaagtgt aggcaagaac tataagttta ttgaaaggta
     4921 tatatccgag gatttgtggg agaaactttt gtccacctac cggatggatt cctatgaaaa
     4981 catatgggaa gcattatttc tatgccatca attgttcagg gcggtatccg gtgaggtggc
     5041 ggaaaggctt cattatgcct atccggagta tgataggaat ataacaaaat ataccaggga
     5101 catgtataaa aaatacactg gtaaaaccgg ctgcctggat agcacatatg ccgctgatat
     5161 agaagagagg cgggaacagt gattacagaa atgaaagcag ggcacctgaa agatatcgat
     5221 aaacccagcg aaccatttga ggtgataggt aagattatac cgaggtatga aaacgagaat
     5281 tggaccttta cagaattact ctatgaagcg ccatatttaa aaagctacca agacgaagag
     5341 gatgaagagg atgaggaggc agattgcctt gaatatattg acaatactga taagataata
     5401 tatctttact accaagacga taaatgcgtc ggaaaagtta aactgcgaaa aaattggaac
     5461 cggtacgctt atatagaaga tatcgccgta tgtaaggatt tcagggggca aggcataggc
     5521 agcgcgctta tcaatatatc tatagaatgg gcaaagcata aaaacttgca tggactaatg
     5581 cttgaaaccc aggacaataa ccttatagct tgtaaattct atcataattg tggtttcaaa
     5641 atcggctccg tcgatactat gttatacgcc aactttgaaa acaactttga aaaagctgtt
     5701 ttctggtatt taaggtttta gaatgcaagg aacagtgaat tggagttcgt cttgttataa
     5761 ttagcttctt ggggtatctt taaatactgt agaaaagagg aaggaaataa taaatggcta
     5821 aaatgagaat atcaccggaa ttgaaaaaac tgatcgaaaa ataccgctgc gtaaaagata
     5881 cggaaggaat gtctcctgct aaggtatata agctggtggg agaaaatgaa aacctatatt
     5941 taaaaatgac ggacagccgg tataaaggga ccacctatga tgtggaacgg gaaaaggaca
     6001 tgatgctatg gctggaagga aagctgcctg ttccaaaggt cctgcacttt gaacggcatg
     6061 atggctggag caatctgctc atgagtgagg ccgatggcgt cctttgctcg gaagagtatg
     6121 aagatgaaca aagccctgaa aagattatcg agctgtatgc ggagtgcatc aggctctttc
     6181 actccatcga catatcggat tgtccctata cgaatagctt agacagccgc ttagccgaat
     6241 tggattactt actgaataac gatctggccg atgtggattg cgaaaactgg gaagaagaca
     6301 ctccatttaa agatccgcgc gagctgtatg attttttaaa gacggaaaag cccgaagagg
     6361 aacttgtctt ttcccacggc gacctgggag acagcaacat ctttgtgaaa gatggcaaag
     6421 taagtggctt tattgatctt gggagaagcg gcagggcgga caagtggtat gacattgcct
     6481 tctgcgtccg gtcgatcagg gaggatatcg gggaagaaca gtatgtcgag ctattttttg
     6541 acttactggg gatcaagcct gattgggaga aaataaaata ttatatttta ctggatgaat
     6601 tgttttagta cctagattta gatgtctaaa aagctttaac tacaagcttt ttagacatct
     6661 aatcttttct gaagtacatc cgcaactgtc catactctga tgttttatat cttttctaaa
     6721 agttcgctag atagagttct atattagaga tgtaaagagt tttggtgaag aggtttgtga
     6781 gtatggcttt tataaccagg gtgcaatgag aagcactaac agcagctagc ttaagaacgc
     6841 ataggatata ctttttatgg aagtcacaga ggaggaataa aatttgaaca ggattaacag
     6901 agtaacctct attcttattc agctgcaatc aaaaaaaata attcctgcca aagaaattgc
     6961 acagcgattt aatataagct taaggacagt ttacagagat atccggacac ttgaagaagc
     7021 cggaatacca attggatctg aggccggaaa aggatatttt cttgtagagg gcttcctact
     7081 tccgccggta atgtttacag cggcggaagt gggtgcattg attacagcag ggaaattttt
     7141 aaattgccat ggagatgaat catttataaa ggattttgat tcagctatgt ataaaatcaa
     7201 atctattttg aagcatggtg aaaaaaacta tgcacaggag ttggagaaca gtattaacgt
     7261 gtacagcaca tctggacaga aaaatacatt ggcggataac gttattgcag caattcagac
     7321 tgcaatctgc aataaaaggg taatatcaat tcaatatcct gcatcaggag ggcaagaacc
     7381 ggaaagcaga atgatagagc ctatatcact ggggttttat gaacagaact ggtacttaat
     7441 cggttttgca ggctaagaaa tgaatatagg aattttcgtg tggatcggat taaaagcatt
     7501 tgcatagaag aagagctctg tcaaagccat gatggctctt tggaacaaat attaaaacaa
     7561 atgctttcat ataaaaaact atataatgta attcttagag ctgaaaaagg ggagacatac
     7621 aattctatca agaacaggta ttctctcggc ttccttgaag aaaccgattt gggaagtaaa
     7681 atggaaatcg aattccagac agattccttt gagatattga gcaagcagct gattgaatat
     7741 ggatcaggca ttgaaatagt ccaacctgat gaattaaaat gcataacccg taaacacctg
     7801 gcacaaatta cgaatcactg cctaaacttg atttagagaa agcgattggt ctgttatgta
     7861 ttttttgtaa ggtttcatat cccctgctga catacagctg tcagtgtatt atgatattct
     7921 actatcaaaa aggaggattg aaaaatatga aaccaaagat aattatgcac acacaaatta
     7981 gccttgatgg ccgcattaaa ggctttgata atccggaagt ttactaccag gttgccgggg
     8041 gaattcattc ggatgcggtg ctgtttgggt ccaatactgt ttttactgca tttgaaaaat
     8101 atcctgctga aacagaggct gatttt
//
LOCUS       DAZUKC010000103         8092 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000103 DAZUKC010000000
VERSION     DAZUKC010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.105"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            116..949
                     /gene="msrA"
                     /locus_tag="ACG80T_002414"
     CDS             116..949
                     /gene="msrA"
                     /locus_tag="ACG80T_002414"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS4842032.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            1029..2018
                     /locus_tag="ACG80T_002415"
     CDS             1029..2018
                     /locus_tag="ACG80T_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS4842033.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            2307..3005
                     /locus_tag="ACG80T_002416"
     CDS             2307..3005
                     /locus_tag="ACG80T_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFS4842034.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            3155..4471
                     /locus_tag="ACG80T_002417"
     CDS             3155..4471
                     /locus_tag="ACG80T_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4842035.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            4488..5903
                     /locus_tag="ACG80T_002418"
     CDS             4488..5903
                     /locus_tag="ACG80T_002418"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4842036.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            6367..6687
                     /locus_tag="ACG80T_002419"
     CDS             6367..6687
                     /locus_tag="ACG80T_002419"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4842037.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            6713..7030
                     /locus_tag="ACG80T_002420"
     CDS             6713..7030
                     /locus_tag="ACG80T_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS4842038.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            7059..>8092
                     /locus_tag="ACG80T_002421"
     CDS             7059..>8092
                     /locus_tag="ACG80T_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4842039.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLV"
BASE COUNT         2662 a         1318 c         1717 g         2395 t
ORIGIN      
        1 tctctttttt atacgaaaaa tctgtagggg gatgaagtaa tcgcttattt cctcacttta
       61 tgataaatta cagctaaagt cactagcttg tcttcttcgg aaaggaggag aatctatgga
      121 tgaggaacaa atattcattc aaacacttta taatctgatt ttgaatccga atacacgaga
      181 ttgggaacga aaagtattga ttcagacaaa gaatgacata agagaaaata tatccgttaa
      241 agaacagtta tcaaaactag aagcaacact acgtccatta gctatccgaa tgaatctgac
      301 gcctgatgta atggactttt atttattgat tacggaagga tttgataagg agcaaaaata
      361 tgacttttcc aagcatgcca tgcaagatgc tgactatcaa gaacgtgcag tatttgcagg
      421 gggatgtttc tggtgtatgg tcgagccgtt tgaatcgaaa aaagggatcc tatctgtttt
      481 gtcaggatac acgggcggac atgttgaaaa accaaattat gatcaagtaa gtggtggtta
      541 cacaggtcat gtggaagctg tggagatcat ttatgacaca agagaaataa gttatagcga
      601 gttgctgaca atctattggc aaattactga tcccacagat acatttggac aatttcaaga
      661 tagagggaag caatatcgtc cagtgatttt ttatcaagtc gaacgacaaa aagaattagc
      721 ggaacaatca aaacagaaac tagacagttc aggaactttc caccaaccaa tcgtcacaaa
      781 aatcgaacca gccggtacgt tttggccagc agaaaactat catcagcaat tttacaaaaa
      841 acaaccgaaa cgttataaaa aaatccagca agcacgtaat caatttctga tctatcaacg
      901 agtaaaaaac aaatggcaaa aaaatatacg aaaaaatcat tttgattaac tccttttctt
      961 taaaatgtag taaactaaaa atagaaaaaa gaaagcgttt taaaaagatt tgatgaggag
     1021 gagaaaaatt gtatattgca gatagtgcac gttatgaaaa aatggaatat cgtaaatccg
     1081 gtcattcagg tttgaaatta ccgattttat ctctcggatt atggcaaaat tttggtgatt
     1141 atgatcctat ccataatcaa agagagattt taagaggcgc attcgacatg ggaattaccc
     1201 atttcgacct tgcgaataat tatggaggcc ctgctggagc tgcagagaaa aactttgggc
     1261 gtatttttag agaagatttt caagcctacc gtgatgaact gattatttct agcaaagcag
     1321 gttatcatat gtgggaaggg ccatatgggg aatggggctc tcgtaaatcc atcatttcaa
     1381 gttgtgacca aagtctccaa agaatggggc tagattatgt tgacatcttc tatcatcatc
     1441 gcccagaccc tgatacacct ttggaagaaa cagcagaagc tttgatgcaa cttgtaagac
     1501 aaggaaaagc attatatatt ggtatatcca actataatgg agaagataca aaaaaaatga
     1561 ctgaaatttt aaaaagaaag gaagcaccgt tcattattca tcagatgcgc tacaatatgt
     1621 tttctcgtgc actactagaa gacgatctat ctccagtatt agaagaagaa gggctagggg
     1681 caatcacgtt cagtccgctt gctcaagggt tgctgacaaa tcgttatcta catggtattc
     1741 ctgaagattc tagagcacat cgtaaggaaa taccattcct ttcagaagaa caagttggat
     1801 caactctgga aaaaatcaaa gcactgcaaa caatcgccgt atcacgagga caatcattag
     1861 cgcaaatggc ccttgcatgg aatttgcgac aaaaatctgt tacaagtgta ctggtcggag
     1921 caagtcggtt aagtcagtta caagaaagtg ttcgaatgat ggataattta gatttctcac
     1981 cagaagaaga attgagaatc gaccaaattc tagaatagaa acgatgtaat caaacttttt
     2041 atccatatat taaaaaaaac aaaagatgac atcaagaatc gaatgaagat cgtctgttat
     2101 tgaacatcat attttttgtc gttcaaaata aaaatgatcg aaggctagga gtgaatttta
     2161 tcctagtctt ttttatggta aatatcttct tcgtttgtat gtctatgaac ggattaatca
     2221 attttgtata caaaaaatca tcagatgatt caaacgtgtt ataataatta cgaaaaaata
     2281 catcagatga aaaggaggta tgtggtttgg aagaaaataa aaccctagta gaagtaacta
     2341 ctgacaagat ccttagatat ttaaaggaaa tgcagttgca agtaggagat aaactgccga
     2401 atgaatatga attgtccgat tatttagagg tggggcgaag cacggtgcga gaagctgtgc
     2461 gggctctagc ttccaggaat atccttgaag ttcggcaagg agcggggaca tttgtcagtg
     2521 ataagcgagg gattgccaat gacccactcg gtttttcttt gattgaagat caggatcgga
     2581 tgatacgaga cttatttgaa ctgagatatt tattggagcc taagatggca ggtttggcag
     2641 cggaatatgc aacgaagaat caaatcgtcc aaatgaaaaa attaacgcaa aagattgaaa
     2701 cttcttttcg agaaggcaca caagaacatg ttcaattaga catcgatttt catacgatga
     2761 ttgcagaagc tagcggaaac gtagccttgc tgcatattat cccaatcatt aatgaatcaa
     2821 ttgctttgtt taataaaaat tatgatatcc agcatctgaa aatcgagacg atcgatattc
     2881 atacagagat cgttcaggcc atcgaaaggc ggaatgcact tgctgcgttt gatgcaatga
     2941 ctgtccatat ggcaataaat cggagagaat tacagaaaat aataggagaa aaagaaacta
     3001 attgacagtt catctgatgt attttatgat aaatacatca gatgaatttt tgttttatat
     3061 ttggtaaaaa agaggagtaa atagtttctg ttcatcgaaa tgtaagcgct gactaataga
     3121 caaaaagaga tgacgtcaag gagaagaagc aggaatgagg ggatctggaa tgagttttat
     3181 ggataaatta gccaatgttt tagggaacgt ggcaacgaga atcaataatt taaggtatat
     3241 catggtgata aaaaacgcat ttgctgcatt gattccggta attattacgg gtgcttttgg
     3301 cacattgttt tcagcaatgg tctttgactc ggaaaatggc ttagcacaga taagtttctt
     3361 gcgtttttta gaagaattaa agcctatcgc atctgccatc agctatgtca cactaagttt
     3421 cttgaccatc tatgctgttt ttctgatagg gatcgaatta gccaagctga ataaagtgga
     3481 tggtgtattc cctggtatca tcgcagtgat gtcttattta tctgttaatc caactgttta
     3541 tgaattagtg aatgaaaatc agactgttat agtagaaaac gtattggcta aacaatatac
     3601 agatacgaaa gggttatttt tgggaatgtt ggtctccatc ctttctattg aactttattg
     3661 ctggttgagg aaacagaaga aactacagct gagaatgcct gataccgtgc caacgaatgt
     3721 ttctgctagt ttttctgctt tattcccgac gattctgact gtatcgggta tcgcggctgc
     3781 gggatttatc atcaaagaac ttacaggaat gtacctttat gatatcattt acaatgtcgt
     3841 acaacgccca ttagaaggtg tcgtacaagg gttaccgggt attttgctat tgatgtttat
     3901 tgcgcaagtc ttttgggtga ttggtatcca tggaaatcaa atggtgaagc cgatacggga
     3961 accgttgctt ttagcggcaa ttgctgtaaa tacggaagct tttgaagctg gaaaagaagt
     4021 accgaacatt attactatgc cattttggga tatgtatatg agtatgggcg ggtctggagt
     4081 cacgatcggt ttactgattg cagtattgtt agttggaaaa cgggaagata tgaaacaaat
     4141 caccaagtta tcccttgctc ctgggatctt caacatcaat gaaccagtta tttttggtat
     4201 gccgatcatg ctgaatccaa ttttagctat tccgtttatc ttaacgcctc ttatcactgg
     4261 aacgattggt tattttgcaa ccgctttagg atttgcggcg aaagcagttg taatggtccc
     4321 ttggccaatg ccgccgatcg tcaatgctta tctggcaaca gcaggaaata tcggtgcagt
     4381 tattacgcaa cttgtttgta ttattgtttc tattttgatc tatctgccat ttgtaaaagt
     4441 agcaaatcgt gtggaaaatg atgggatcta aaaaacagga ggaaatcatg aaaattacta
     4501 ttccaaaaaa ttttattctt ggtgctgcat cttctgcttg gcaaacggaa ggttggaaag
     4561 gaaaaaaaga aggacaggat tcgtttatag atagctggta taaaaatgaa cattttgttt
     4621 ggcataatgg gtatggccca gccgtagcaa ctaattttat ggaacaatac cagcaggatg
     4681 tggatctgat gaaatccatc catctgacac attatcggac ttctatcaat tgggctcgtt
     4741 tttttactga ttatgagaac tgtatagtag atgaagaata tgctcagcat ataagtgaaa
     4801 tgatcgactc tttgatcgaa gcagacgtag aaccgatgat ttgtttggaa cattatgaag
     4861 tcccagcttt tcttttggaa aaatatgatg gctggtcttc aagaaaagta gttgattttt
     4921 atgctaagta tgctcaaatt gcctttgatc gttacggtga tagggtaaaa aactggttta
     4981 cattcaacga accaatcgtt ccccaaacgc gttgctattt ggatgctgtt cgctggccgc
     5041 atgaacaaaa cacaaaaaag tggatgatgt ggaattatca taaagtttta gcaacggcta
     5101 aagcagtaga gatttaccgc agcggaaatt atggtggaag gattggctgt attttaaatc
     5161 ctgaaatggt ctatgctcga tccaattctc cagaagacca aaaagctgct tatatgtatg
     5221 atttgtttta taatcgagta ttttttgatc cgatggtaaa aggatactac acagaagagt
     5281 tcctggagtt gtgtcaaaaa caatcgattt atttcgatcc tgcaacagag gatttagaaa
     5341 cgattcagca tcatacagta gatttccttg gaatcaatca atattatcct aaacgcgttc
     5401 gcgcccctcg ttatcaatgg aatgaagaga cacctttcca tcctgaacga tattatgaag
     5461 tctttgattt acctgggaag aaaatgaata attccagggg atgggaaatc tatccgaaaa
     5521 tcatgtatga tatagccatg tatctaaaag aaaattatca taatatccct tggctgataa
     5581 cagaaaacgg tatgggaaga gaatatgaag aacagtatat ggatgagaaa ggaatcgtgc
     5641 aagatgatta ccgtatcgaa tttatcggac agcatttgca ttacttgatc aaagcaacag
     5701 aagaaggcgc aaattgcgaa ggctatatgt tatgggcatt cactgactgt gtgtcaccaa
     5761 tgaatgcatt taagaatcga tatggattag ttcgaatcga attgaacgat gaacgaagca
     5821 gaacattgaa aaaatctgct tcgtggtatc agactttgat tgataagaga gaattggatt
     5881 tgcctgacga aatctataaa taaatagaag ttttttgtag atgttcattg tatttactgg
     5941 ttatttgaaa taaaagaggt tgtgaatcca gcgttttgag ctaataccca gggtcagctg
     6001 attgaacacc gtttattgag aggttgtgaa aaagctgtcc tgcatcaagt aataagaata
     6061 gcaaagtaaa aacaacatct tatgttttta acttggttgg acttattacc gcagatgcaa
     6121 gcttttgaat accgtttatt tgaaaaaagt tgtgacaagt tttgtgtcac aacttttttt
     6181 gtgcacgtta aaagaaaaaa ggtaataaaa attaatttgt gtgtttagtg tgcgctttct
     6241 tttttagtgt gtatgcaact tggtatagac cttaaatgtg atgatatcaa ctttttaaag
     6301 cgcttttaaa gttggcacgc tacttgctta tatacaagtg taagtataga aaggaacgtg
     6361 tttaaaatgg aaaagaaaac aatcatgctt gtatgttcag ctggaatgag tacaagtctc
     6421 ctagtcacaa aaatgcaaaa ggcagcagaa gcaaaaggaa tggaagcaga catctttgct
     6481 gtatctgctt cagatgcgga taatcaacta gaatctaaaa atgtagacgt tttactttta
     6541 ggaccgcaag tacgctttat gcaaaatcaa tttgctgaaa agctagcacc taaaggcatt
     6601 cctttagatg taatcaatat ggctgactat ggcatgatga atggtgaaaa agtactcgac
     6661 caagcagaaa aactaataaa caaataataa aaattatcgg aggatacaat aaatggaaga
     6721 tcaaaaaaat ctagaagcca tcatgggtct gatcatgttt ggcggaaatg caaaaagcga
     6781 tgcaatggaa gcgattgctg ctgcgaaaaa aggtgatttc actttagcag atgaaaaaat
     6841 caaagatgca gaggaatctt tagtccaagc acatcactct caaacagaga tgctgacaca
     6901 agaagcacaa ggaaatcacg ttgaagtcac gcttttgacg gtccacagtc aagatcatct
     6961 gatgacatct atagctttta cagatttagc aaaagagatc attgatctgt atcgtcgatt
     7021 gaacgcgtaa tctacagtaa agaaaagagg gaagatttat ggataaattt gttgcattta
     7081 tggagaaaca ttttatacct gtagcatcaa aaattggtgc acaacggcat ttagttgcga
     7141 ttcgggactc atttatggtg agtatgccgt taatgatctt aggagcatta gcagtaatga
     7201 tcaataactt accgattccc gggttccaag aattgatgaa ctcaattttt ggtggtgaat
     7261 catggaaagg attcggggct gctgcttgga acggtacatt tgccattctt tctgtcttga
     7321 ttgctttctt gcttgcttat catttagcta acggctaccg aaaagacggt gtttccgcag
     7381 gtgtgatttc tttaggctct ttcttcgctt taggcggagc attaggtatg agctcaaacg
     7441 gtttgtttat tgctattatt gttgggatta tttcaacaga aatctttgta cgtttaagcg
     7501 gaaatgaaaa attgatcatc aaaatgcctg atggtgtgcc accggcagtt gcaaaagcat
     7561 ttgcttcatt actaccagca atgatcacta tctctgcgtt tgctttagtt gctgcaattt
     7621 ttgctgcatt cggtgttgat gatatcgtag gttctttcta tacagtggtc caagaaccat
     7681 ttatgggact tgcaaactct tatccatctg ccttactgct tgcatttatc acaccattct
     7741 tatggttctt tggtttacac ggagcgaata tggtcgatcc tttgatgcaa acaatcaatg
     7801 cgccagccat tgaagcaaac gttaatgcaa tcgcagctgg acatagcgca ccgtttatcg
     7861 tgaacaaacc tttctttgac agctttgtca acttaggagg aacaggtgca actttaggtt
     7921 tactgttagc tatttattta gtaggacgta aaaacaaacc atatatggtt gtaacaaact
     7981 tgtcgattgc gccaggtgtc ttcaatatta atgaaccaac gatgtttggg ttacctatcg
     8041 tgttgaaccc gatcatgttc attccattta ttttgacacc aatggtctta gt
//
LOCUS       DAZUKC010000104         8029 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000104 DAZUKC010000000
VERSION     DAZUKC010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.106"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            34..903
                     /locus_tag="ACG80T_002422"
     CDS             34..903
                     /locus_tag="ACG80T_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFS4842040.1"
                     /translation="MKKKEFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            923..1270
                     /locus_tag="ACG80T_002423"
     CDS             923..1270
                     /locus_tag="ACG80T_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842041.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            1283..2545
                     /locus_tag="ACG80T_002424"
     CDS             1283..2545
                     /locus_tag="ACG80T_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842042.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            2625..4001
                     /locus_tag="ACG80T_002425"
     CDS             2625..4001
                     /locus_tag="ACG80T_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFS4842043.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTAMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMIIKLVNGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAESRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANSSSKEPSIEKKSTFALKLEKNKEEKEKQGAKTLEKLQAEKLEKVLKALDK
                     ADSEPQTSNNTNNEQPISSKTKPKTTDPKVSVNVTITTDKSEKKQVNGTTSPIMQKML
                     KAKKQVHK"
     gene            3998..4627
                     /locus_tag="ACG80T_002426"
     CDS             3998..4627
                     /locus_tag="ACG80T_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842044.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRTKKRDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNDRKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            4646..5125
                     /locus_tag="ACG80T_002427"
     CDS             4646..5125
                     /locus_tag="ACG80T_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842045.1"
                     /translation="MLELFEANRTGFNQKLEVGETIEYKDKNYIIIGIYNTRINRIGD
                     PRITSDLVCQSVNYSPDLTSRYKKYVLLEYRHKITDEIGVNNTRNIFKIGTVIPYSNN
                     EEKIFYQIYGIEKFEYEHVDLLVTYQMRLIEPWSQAEIDKAVKLNRLSKFNVLGNAF"
     gene            5684..6283
                     /locus_tag="ACG80T_002428"
     CDS             5684..6283
                     /locus_tag="ACG80T_002428"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFS4842046.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLHSNIVSMGLSGGIFGWIALFAIAYIRFDSKKHLLLSVFVLLIIGIL
                     LVSTFESGETNVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            6299..6775
                     /locus_tag="ACG80T_002429"
     CDS             6299..6775
                     /locus_tag="ACG80T_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842047.1"
                     /translation="MPKKKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRIFEWLNTNTEQEPLRAISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSEDSKSMRDLLITYLNEHEFEQTKVKKKNKK"
     gene            6792..7694
                     /locus_tag="ACG80T_002430"
     CDS             6792..7694
                     /locus_tag="ACG80T_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842048.1"
                     /translation="MLFTLLGYLCLVLAFFGAIAIFVGQFQANNPIMRNIGIGIVIIS
                     LIVFGATKIFGSNDQQFGISGVDNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDDK
                     KASDDVLATIKPSQSQFEVFYTVKENNPVRKLTLVFNVDGEEYYKDVKINKASTKKIV
                     ESAKKEVATEESTTESSSTVEVELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQRINVTLFNAVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGSDLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            7767..7961
                     /locus_tag="ACG80T_002431"
     CDS             7767..7961
                     /locus_tag="ACG80T_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842049.1"
                     /translation="MVVNKEKVRKYVTLKIASVEYLEKRAKEEEKTRGKKVTVSELLE
                     ELIESDQTIRRAFTNRSVKS"
BASE COUNT         3063 a         1141 c         1615 g         2210 t
ORIGIN      
        1 ttattatgat aacacaataa aaggaatgat ggtatgaaaa agaaggagtt tagtcgatcg
       61 ttgttaggaa ttttagtttt agttgcggtt ggtcttagtg gcaagggtta ctttcagaat
      121 aacagcactc aggaacatga ttttcagatc aaaagtagtg ttcaggtagg taataaagca
      181 gacagtgaat caaaagaatt tttagggata cgttatgatc caaacaaata tccaaataat
      241 atcgtaaaca ttaatgatgg gaaatataca ctgacaaaag aagaccatga agcattatca
      301 gaaaaaggca gtcaaacctt ttgggtaaat tatgcagatt tagatcaact tggaagagca
      361 ggagatgttg aagcgttgat cacctatgat gctgtaaatt ctcactcatc taaaaatgtg
      421 gaacgtccat ctttcaaaag ttctgtccat attgcaggag aatacaagga tggagtattt
      481 gatcaagaga gagatacttg ggtcggcaaa gattcaaaca atgaaatcac tcagctagaa
      541 ggatataaag gttatctcta taacaaaagt cattcattag catggtcgct aggaggagat
      601 atggaggtac acaatttaac acttggaacg agagcgcaaa atgttgggaa aaatgatcag
      661 aatggaggga tggcttactc agaaacgatt gtacgtgatg caatctataa aaataagaat
      721 gaaaaggtat tgtataaagt tgatccaatt tataaaggga atgaattggt tcctcgtggt
      781 tctcatgtcg aagctttctc agttaacgat aatggaaaaa cggtaaacct taatgtttat
      841 gtatttaaca atcaaaaagg aatcaacatc aactatcaaa atggtacttg ggtaagagat
      901 tagaaattga gaaggtgaag aattggaaac aataagcatc aatggattag ataaatcagt
      961 tgcaaatcga ataaaaaaag gaaatggcta ctatcttctg aagaagggta aatattcagt
     1021 tgctagagag ttagaaacaa tgagtatcgt gtatgtagct aagaatcatt ttgacgtaga
     1081 acaggctatt tttgatccag attctgaaat agggaaatgt gttgaattat cagagaaaac
     1141 tgttagtaca aaaggggcga tgatcattga gagagaaatg ttagacgaaa tcagagtaat
     1201 cattcctaaa gatacgcaga tgtgtgagga cagcataagt gacgatgaac caaaatcaga
     1261 aagaaaataa ggagatattc agatgagcga aaaatccatt gaattaaagg aatataagcg
     1321 aagcgatgga gaaaaatatt atcctgtagc cagtttttct aaatcagtat atctggttgg
     1381 acgtgcattg gtagttggtg caattggcat atatattttg cattgggtaa caaaggttgt
     1441 acaactgctt gtagatacct tagtaagtat tcttgtctcg ttaaatgatg ttaacggaac
     1501 agatgattta ggattaaaag aaatgtttaa agagttactc aatgcgtttt ttcccaatta
     1561 tttaagttat tcatataatg tacaaagctt ttttgcaaag ctacacttgg tagacatctc
     1621 tattttagta ttagaagcag gaacggtatt ctttgttgga tatttggtta ctttgatgtt
     1681 caggcatcac tatggagaac aaagaccatt ttttgatgat atggaaagta gaaggttgaa
     1741 aagagaagct ctagaagcta tggatgtgaa gtatgatcca aacaagatta taggagatga
     1801 aacgaacaac aaaaaaagaa aacgaaacaa aacgcaatta gaatccgatg tgctatcaac
     1861 gcttaaaaat atcaaaattt ctgttcatac aagacgtgat ctaagtgtca atgaagatat
     1921 tcgagaatat atcgtaaaaa tcccgcaacc aaaagggaag aaagttagag aaacagtttt
     1981 acgacaaatt caagatttag ggtcgatcct gacaagagca acaagcggac gagccaaatt
     2041 tggtgaaaaa gaagatgaag tttcaggcga ttatttcatt ttccgagcat cagtaaatat
     2101 tgaaagagaa cgtaaggaaa gagtaagtac caaaaacaaa gaaacggaaa gagcaacatc
     2161 ttcaatcgca gtttcaaatt cacaattttc ttttccaatc aatctctttg ccgataattc
     2221 caaaaatatc caaaaccaaa ccgaaaaagc tagaaaatat gccgaaactc aaaagcaatc
     2281 tttagaactt tatctatctt ctgctgacgt acctgcaagt ctaacagata tgcacgttgg
     2341 gaatacatct attgaatatt tgtttacttt atctcgtacc agaaaaattc aactaagctc
     2401 aatgaaagaa gaaattgaaa cttacttgca tatatcaggt gtaatcgtgc ggatcaacgc
     2461 agggcgtatt caaattactt taccattagg agaaaatgat cgtattccaa ttgatgtacc
     2521 atcaatgatc aatagcgtgt tttaaaagac atcacaaata gaaacagaaa atacaaacta
     2581 ccagttcaca aacagaaaag taccgagaaa agtgaggaat agaaatggct atgaaattat
     2641 tagagaaagt caaaaagaga gccgaaaaag ctgaatctat tttgctagat aaagaaccta
     2701 atataaagct taatggaaaa ttcagtgtca atatgttgaa cacaaaggct aaagacccga
     2761 cagatgctat ttttggaatt aagcctgatg gcacaattgc tcatttttgc atcagcgaaa
     2821 tgccacacat gttggttgca ggaacgacag gatcagggaa atcagtatta ctgaatgaaa
     2881 ttcttgttac agcaatgtgt cactcaacgc ctgatgaatt aaaaatagga attgtagacc
     2941 caaaaagggt tgaatttgga agatacaaaa aattgccgtt catgttggct gatccaatta
     3001 cagatatgga tgaagcttat gatttttttg aatatcttgt gatcttgatg catgaacgtt
     3061 acaaactgat ggaaaaagca gggttacaaa acatcacgct atataacgaa tatgccgaaa
     3121 aaaatggatt agatagattt ccctatgtga tcttattagt tgatgaatat tcacagttgg
     3181 ttggaacaca taaagaagta gaaggtttga ttgttcaatt aggacaaatg gcaagggcag
     3241 caggtattca tgttatctta gctacacaat ctcctcgatc aacagttgtc actggtatta
     3301 tcaaagccaa ttttccgtca cgtgtttgcc ttatggtagc aagtgattta gaaagtagga
     3361 tcgtattaga tgaaggcggt ggagaaagcc taagtccaaa gggagatatg attattaagc
     3421 tcgttaatgg aagtatggtt agagcgcagg gagcttacat cagtaacaaa gaaattgaaa
     3481 caattttcaa tcaccttaga aatactatgc cagaaccaga atatgttgat taccgagcaa
     3541 tcgtagctga atctcgtggt gagggtggag aagacgagga ttttggtgga attggtaaag
     3601 ctcctgtaag aaaatctgct aaaagaaatt tgaacacacc tttagcaaat tcatcatcta
     3661 aagagccatc aattgaaaag aagagtacgt ttgctctaaa acttgaaaaa aacaaagaag
     3721 aaaaagaaaa acaaggagct aaaacactag aaaaacttca agccgaaaaa ttagaaaaag
     3781 ttttaaaagc attagataaa gctgattctg aaccgcaaac ctcaaacaac acgaataatg
     3841 aacagccgat ttcatctaaa actaaaccga aaactactga tccgaaagtt tcagtaaacg
     3901 tgaccatcac aaccgataag tctgagaaga aacaagtaaa cggaacgact tcacctatca
     3961 tgcaaaaaat gttgaaagcc aaaaaacagg tgcataaatg atcattgtca gttctttttt
     4021 gaggtggctt acgccatata aatatacaga tgaagtagtt gtgaaacgaa cgaagaaaag
     4081 agataaaaaa gcggtactaa ctcctaaaca gatgcttgat catggcattt taggggtgaa
     4141 ggttttagaa gtgaataaac cgtttgaatc aaatagtgca agagaaaagt tgcttggagc
     4201 tgaagccgat gtcgcatatt tttaccatga caaaagcaag catcaaatag ctattaaatt
     4261 caatccaaaa tcggcatggc ggtatcaagc aagagatgac ttgaaacgta ggaaacttag
     4321 accaatgctc tatccagaag gaaaagaagc atttgataga acccacatcg ttcccattgg
     4381 ttttcatggt acagaatcag ataaccgcct attagtaggt tggaacagca atcaaaatag
     4441 agttcagatc aataaatttg agaagaaagt aaggaaattc aacgatcgaa aaacgatcct
     4501 atggtttgca gatatttgta aacagccgga tggaacagca aagtgggaag cggtgatatt
     4561 tgacgaaaat ggtaaaaaag taatgtctga aacatggcat gataaagcta aatttgtttg
     4621 gcagtagcag tagaggtgaa aaagattgct agaacttttt gaagcaaata gaacaggctt
     4681 taatcaaaaa ttagaagtgg gagaaactat agaatataaa gataaaaact atattattat
     4741 cggaatctat aacacgagaa ttaacagaat aggtgatcca cgtataacca gtgacttggt
     4801 ttgtcaatct gttaactatt cacctgatct gacaagcagg tataagaaat acgtgttatt
     4861 agaatataga cacaagatta ctgatgaaat tggtgtaaac aacacacgaa acatatttaa
     4921 gatcggaaca gtgattcctt atagtaacaa cgaagaaaaa atattttatc agatttacgg
     4981 aatagaaaag tttgaatatg aacatgtcga tctgctagtg acgtaccaaa tgcgtttaat
     5041 agaaccttgg tcacaggcag aaattgataa agcagtaaaa ttgaaccgat tatcaaaatt
     5101 taacgtatta ggaaatgcct tttaaaaatc gcaatacaca aacagaaaaa taccaaaaaa
     5161 ttagcaatga acgtaggtaa gaacatgaca ataaataata ataaccctaa aaaatataat
     5221 gctcttgtcc aatgctatat cgaaaacaaa attacaatgc aagaagtcgc tgatgaatta
     5281 ggcaaatcaa agagcctagt atcacacgat tttaagcaca atgtttttcc gaacccgatt
     5341 gagctgagga aaaaatttca aaagagagtt tatcaagaaa taggtaagtg gattgatgaa
     5401 gggaatgcaa ttgacccttt aattgaaaaa tatccttttt taaaaagcaa aaataaaatt
     5461 caaaggttaa ttcgtcaagg cgatattcaa gctaaagaac ctgttttgac aaccaatgat
     5521 ttggctaaaa taaatcttca taaacaaatt attgaagact ttgaaaaaaa tctttcaaca
     5581 actgaaattt taaaaaaata caatattccg agaggaaagc taacatcgac tctttttaga
     5641 gaatatggag aatcggcagt tactaaagca aaggaagtag cgaatgagag aaaaaaacaa
     5701 aaatagtagt cgatacctag tagcgactgt agtttttgtt tgtgtattga ttttcctgtt
     5761 tgattttttg cagattggaa aaagcataat tggaaataca ttgagtgcaa aggaaatggt
     5821 tgctttaggt ggtttgtatg aaggacactt gttcccgaat atattcattt ctatcgtttc
     5881 tcatattaac ttgtaccatt taatttctaa tttattgttc ttagtagttc ttgaagaagc
     5941 actgaatgat cagtatgagt ggttaacatt attgatcgtt ttaatcatat caggatttct
     6001 aggaagctta gttccttttc tgctacacag caacatcgta agtatggggc ttagtggagg
     6061 tatttttgga tggattgctt tatttgcgat tgcatacatc cgttttgata gcaagaaaca
     6121 tttattacta agtgtttttg tattattgat aatcggcatt ttactggtat ctacttttga
     6181 atcaggcgag acaaacgtct tgtcacatgt aatcggattt gtctgtggga tgatcttggg
     6241 atttattcta aagaacgttt cattgatcaa acaaaagaat taaaagggag tgctaaaaat
     6301 gccaaaaaaa aaagaaaaga aaattccagt gtttttgtct gtctcagaaa tgttatcact
     6361 tcaaatactg ttaagaaaaa agattgaaac aatagacgaa gaagttagaa ataaaagggc
     6421 tatgtacgaa caacgtgaag cgctaagaga acgaatccac tattttgatg atatacacac
     6481 taaaatctat gaagcacgta caaatcacga agacgaaagc aaattaggat tagctcaaca
     6541 acgaatattt gaatggctga atacgaatac tgagcaagaa ccgttaagag caattagtaa
     6601 tctttttgaa tttggaacga agcattggtt taacgctgta gaaaaggatg ctgacttaca
     6661 agaagcgttc gatacactaa gcgaggatag caaatccatg cgagacttat tgatcactta
     6721 tctaaatgag catgaatttg aacagacgaa agttaaaaag aaaaataaaa aatagaaagt
     6781 tgtgatgtat catgctgttt accttattag gttatttatg tttagttctt gctttttttg
     6841 gagcaattgc tatatttgtc ggtcagtttc aagcaaataa tccgataatg cgtaacatag
     6901 gcattggaat tgttatcata tccttgattg tatttggtgc cacaaagatt tttgggagta
     6961 atgatcagca gtttggtatt tctggagttg ataatagtta tgctccagat tcattgggaa
     7021 gagtagtgat agatggaacg tttgagaatg taacgagcta caaaaatgtt tcattgacag
     7081 tgaaagaaga tgacaagaaa gcatcagatg acgttctcgc tacgatcaag ccgtctcaat
     7141 cacagtttga agtgttttat acagtcaaag agaataaccc tgtacgcaag ctaacattag
     7201 tttttaacgt tgacggagaa gaatactata aagatgtaaa aatcaataaa gcatcaacga
     7261 aaaagattgt cgaatcagcg aaaaaagaag tagctacaga agaaagtaca actgaatcaa
     7321 gttctactgt cgaagttgaa ctaccagata aaattgattt tgatcctgaa aaagtcaaat
     7381 cgtttgaatc tgtttctggt tctatggcag atcggtttaa cactgaagtt ttcaaagcta
     7441 tcaaaactgt taaattggat actgaaaagc aaaggattaa tgttacctta ttcaatgcag
     7501 ttgaatcaat ggatcaggat aaaaaagtta cggttgcaag agaactaggg aaaagagtaa
     7561 ttgggttgta tcgaggtacg gcaaaaccag attcagacgg ttcagatttg ccagttcacg
     7621 tagtttatga agacggtaaa gtttttggag actctacagc aaatgaagca ctaacgatta
     7681 aattagctaa atagccggaa aattataata aacaagtggt gtcacaccac catcgcatag
     7741 gtgtgacacc ttggagatga ttactgatgg tagtaaataa agaaaaagta agaaagtatg
     7801 ttactttaaa aatagcttct gttgaatatt tagaaaagcg agctaaagaa gaagaaaaaa
     7861 caagaggtaa gaaggtaaca gtttcagagt tgttagaaga attaattgag agtgaccaaa
     7921 caataagacg tgcatttacc aataggtctg ttaaaagtta gggtgatact atctttaatt
     7981 tcaaccaaat ttctaatata tgatgaatag tttccttgta caaaatatc
//
LOCUS       DAZUKC010000105         8013 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000105 DAZUKC010000000
VERSION     DAZUKC010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.107"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            383..571
                     /locus_tag="ACG80T_002432"
     CDS             383..571
                     /locus_tag="ACG80T_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFS4842050.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            649..1662
                     /locus_tag="ACG80T_002433"
     CDS             649..1662
                     /locus_tag="ACG80T_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4842051.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(1729..2763)
                     /locus_tag="ACG80T_002434"
     CDS             complement(1729..2763)
                     /locus_tag="ACG80T_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4842052.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            2878..5103
                     /locus_tag="ACG80T_002435"
     CDS             2878..5103
                     /locus_tag="ACG80T_002435"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFS4842053.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            5121..7004
                     /locus_tag="ACG80T_002436"
     CDS             5121..7004
                     /locus_tag="ACG80T_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFS4842054.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            6985..7959
                     /locus_tag="ACG80T_002437"
     CDS             6985..7959
                     /locus_tag="ACG80T_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFS4842055.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         2585 a         1463 c         1698 g         2267 t
ORIGIN      
        1 agttagtcta cattattttg gcgatttgcc aataaggact gttgagttgt gcaaaatcaa
       61 atctgggtca gaatgaacgt cattctgtct cagatttttc gttatttcaa tttgattaac
      121 tgattgatca aaaagatttt tattttcata ttctcaaatg attcaacact aaatatacat
      181 gtttgacgcg gaaattatta ttcgattttg gcggcatcag gggggatgac gacgtagcag
      241 acccattatt ttactcaaga aaatgtccgt gcctattatt tagaatacga tagtgaacgt
      301 gctgggagtt ttgttctttt agagaaagtc tctgaagata aattagttgt cttagatttt
      361 gactgtaacg aaaaatggcg aaatggaaga gcaccagcaa gtgattgacc ggatctacga
      421 agcgacacga tatctgcctt tggatcgttt gtgcttaagt cctcaatgtg gatttgcttc
      481 aacagaagaa gaaaatgttt tgtttgaaga acaatggaaa aaatttcttt tgtcaaaagc
      541 attgcagaag aagtttgggg aaaatcatta aatcatctag gagagcttga taggaaataa
      601 cactatggta taatcagaat gcttaatact aactactgag ggagccttat gaaaaagaaa
      661 attcttaaat ttatcggatg gttcttcgga atcattcttg ggatcgcgtt tgcaatcttt
      721 cttgccttcc agctttcccc cagacctgga gcattcttga tcaaccatat gttttcaaat
      781 actgttcaaa tcactgataa aaaaacgttc gatcaagcag aaaaaaacgt ggcgaaaaaa
      841 acagatttga cgtatgagtc caagtataaa gagaatactt atgacatcta ctatccaaaa
      901 aacagcaagg ggcctgttcc tgttctattt tgggtacatg gtggaggatt cgtcggcgga
      961 gataaatcgg gtgtcaaaga atttgcgaca aaattagttt cagacgccaa catcgcagtg
     1021 gttgcgatga attatgaatt agcgcctgat tcagaatacc ctaatcaagt tcttcaagtg
     1081 aatgaactga tcaaagattt attagctgaa gaaaaagacg acaaattgct agacatggaa
     1141 caattatttt ttggtggaga tagtgcaggt tcacaaatcg cattgcaata tgcagctata
     1201 caaacgaatg atgcgtatgc taaacaaatg aaaatcaaac agctacttcc aaaagatagt
     1261 ctgaaaggaa ccatttctta ttgcggacct gttgatttga agctaacagc acagcagcat
     1321 tcggatgatc gatttatgaa gtttttcgtc aaaaccgttg cgtggtcgtt gattggaaca
     1381 aaagattgga aaacgagtga gcaattgaaa gaagctagtg tggctgacca tgtttctaag
     1441 gaatttccgc caacgtatat aactgacgga aatgcatact cattccagga gcaaggtcaa
     1501 gcattagaaa atcggttgac agaattaaag gtacccgtgc aatccttgtt ttatacagat
     1561 acaaaaaaag agattaccca tgaataccaa tttgattaca cgctaaaaga atcgcaaaat
     1621 tgttaccagc agacattgga ttttgtgaat aagtataagt aagaggttgt gaaaaagccg
     1681 tttcgttttg agtatgaaga aacgaagcgg cttttcacaa cctctctttc aatacctttt
     1741 tgttcttggg ataaaacttt ccctaacaat caatcgtgca ggataagtca aaatgattgg
     1801 ttcgtcccgt ataccgtcaa ttcgctccaa tgccattcga acagctgctt gcccaattga
     1861 tttcgcattg atttgaacac tggtcaagcc aggggagagg tttgcgattg tctcgatatt
     1921 atcaaagctt gctacaacaa tctcttctcc aatttggata tttttttgtt tcaatgcccg
     1981 atagacacca attgccattg gatcgctccc gacaagaatg gcagtaggaa gaggggattc
     2041 attttctaat agattagtcg caagtacctc accttgtttt tctgtccagc cacctagatg
     2101 ataagacata tatttttcta agtgatgatc gatcataaaa tgctgatagg cacgcatcct
     2161 cttttcattt atagaaatct gcttcccacc gaattcatcc atatcgactt tatatccccc
     2221 gatataggct attcttttat gaccattttc taagaacaat tccagtacgg ttttagtcat
     2281 ccgttcaaga tctgcataca ccatatcgac ctcttgctgg atgtcagggt tatccacgac
     2341 gactatttgc atgttttgtt ttaacaaggc ggtaatcgat tctttcgtaa cagtcccaac
     2401 aacgatcacc gcacctaact gatctagatc ttcccattgt ttaggattat cagatagatt
     2461 atagattcga ttcatcccta aatgcagacg tcgagcttct tcttccatcc ctactcgcaa
     2521 atgtttgtaa tagatatcct gttcttcgct cgctttgccg atcgtagaga tcacaccaat
     2581 cgaaggcatt ctctttttga tatatttacg agtactgatg tcataatcca acgcagcagc
     2641 tgcctctact atccgttgcc gagtagtctg agccacatgg atcgtctgat cgttattcaa
     2701 cacacgggaa actgtagcag gagagacatt tgcctttcgt gcgacatctc taattccaac
     2761 catttttaca cactcctcaa aaaaataaaa aatttttatt gacaaaattg tttagtaaac
     2821 agtatacagg aattataaaa aaaagtaaac ggtttccgtt ttgttgggag tgaaatgatg
     2881 gatattacgc atagaaaaac agataaaaat ttcatcttat ttgatgaaaa gagccgagtc
     2941 ttccatttac gaaatacatt tctttcttat ctgataaaga tagaagaaag caatgtattg
     3001 gcgcatattt attttggcaa accagtcaag cagtacaaag acaataaaaa ttatccccga
     3061 agagatcgtg gattttcagg aaatgttccc ttaaatcctg accggtctct gtcgaaagat
     3121 acactaccgc aagaatactc ttctcatggc agcatggact ttcgaacgcc cgcatcgatc
     3181 attcagcgca agaatggatc agacttactg gatctgagat atgacagcca ttatattaca
     3241 gatgggaagc cagagatcga aggcttgcct caaacttatg tgatggacaa gtcagaggca
     3301 caaacgttgg tgatttcact taaggataga gaaacagcga tttatttcga tttgttctat
     3361 accatattta ctgatagagc agtgatcaca cgatctgtaa agataagaaa tgaaaccgga
     3421 gaaacaatca aactagaaaa agcggctagt ttccagctag actttgccca tactaggcga
     3481 tttgatgaag tcattgcttt gccaggagca catgtcaatg aaagacagat ttcacggcaa
     3541 tctgttttat ctggaacaaa agtatttgaa agccgtcgag gaacgagcag tcaccatatg
     3601 aataacttta ttgctttagt tcatcatcat acgacagaaa atactggaga agcgatcgga
     3661 cttcaatttg tttattccgg caaccattct ttcgaacttg aaaaagatca gatcaatcaa
     3721 ttgagagtcg tcggtgggat caacagccat cgtttttcgt gggaattgaa tgcaggccag
     3781 tcttttcaaa cacctgaaat gatcctttct tactcaagtc agggattgaa caaaatgagt
     3841 cagatccacc atgagttgct acgtgaaaga atcgcaagag gtcgtcatca atttgctgaa
     3901 cggccgattc tggtcaataa ctgggaagcc acttactttg acttcaatag tgaaaaaatc
     3961 aaggcgatca ttgatgaagc aaaggagcta ggcatcgaaa tgttcgtatt agatgatggc
     4021 tggtttggaa aaagagatgc ggacaattct tcattaggag actggttcga gtatgaaggc
     4081 aagttgacaa atggattacg tgaaatagca gattatgctc atagcaaagg attgaaattt
     4141 ggtttatggt ttgaacctga aatgatctcg gttgattctg aactgtatcg aacacatcct
     4201 gattttctta tgcaggagcc aggtagaatg ccttctgctt ccagatcaca acatgtactg
     4261 gattttacaa ggttagatgt tcggcagacg attgagaaac aaatgagaaa gattttagat
     4321 acgattcctc ttgactacat caaatgggat atgaaccgtt cgctttcaga tgtctattcg
     4381 attacccttg acccacagag acaaggagaa gtagcgcatc gttatatgct cgggctttat
     4441 gaactacttg aacatttgac aactgattat ccggaaatct tatgggaagg ctgttctggt
     4501 ggaggtggac ggtttgatgc cgggtttatt tattatatgc cgcagtcatg gacaagtgat
     4561 aacacggatg cagtggagcg tatgaaaatc caatatggaa caagccttgc ttatccgatt
     4621 tcgtccatca cagctcatgt atcagctgta ccaaatcatc agaccgacag atctacttcg
     4681 ttgaaaacaa gaggagaaac agctatgagc gctgtttttg gttatgaact ggatttgaca
     4741 aagctgtctc ctgaagaaaa aaagcaagta aaagaccaaa ttatatccta tcaaacgatt
     4801 cgtccggtga tccagtatgg ccattattat cgtttggcga gtccttttga agaaaatatt
     4861 gccgcatgga tgtttgtgtc tcctaaacaa gatgaagcta ttgtgttttt agggaggata
     4921 ctagcttcag ctcaaccagc gttccatgaa gtatatctga tggggttaga tgatgaggca
     4981 ctttatcagg aacagacctc gaagcggata ttttcggggg ccgaattgat gacagttgga
     5041 ctttacttcc ccgattttca aggtgatttc caaacagaac tgcttcattt caaaaagtta
     5101 tgagagagaa ggaaaaaagt atgaaagcaa atataaaatg gttagatgat ccacaagttt
     5161 ttagagtgaa ccaatcaaat gcacatagcg atcatgtttg ttatgcaaca agggaagaag
     5221 tagaaaccac atcctccctt aaacagtctt taaatggatc atggcgtttt tgttattcgc
     5281 caaatccttc ggtacgacca aaaacatttt ataaaaaaga ttttgatgcc tgttcttttg
     5341 atgaaatcac tgttcctagt catattgaat tagcaaatta tgacaagatc cattacatca
     5401 atacgatgta tccgtgggag ggacatgtat ttcgccgtcc ccctttcacg acaagccctg
     5461 agaaagaggc ggaagggatc ttttcagctg ctaaagacaa tccggttggt tcgtatttga
     5521 cgacttttcg attagaagaa ggtctaaagg gaaaacaaat ctcgattcat tttgctggtg
     5581 tagaacaagc aatgtttatt tggttgaatg gtcattttat cgggtatgcc gaagattcgt
     5641 tcactccgtc tgaatttgat ttaacacctt atttggaaga agaaaatatt ctagctgtgg
     5701 aggtttacaa aagaagtaca gctgcattct tggaagatca ggactttttc cgattttttg
     5761 ggatcttcag agatgtcttc ttagttgctc gaggacaaaa ccatttagaa gatctttgga
     5821 tcaaacctcg cttagatgac aaatgtcaaa atggcaaact tgaagtagat ttgtcttttg
     5881 agcaagtagc caaaaacagt cagctgaatg tagcgatact agatcctgat gggaaggaaa
     5941 tctatcaaca gcaatatccg ttaaaagagc aaatgacgat tcgcatcgat ggatttgaaa
     6001 atgtggcact gtgggggcat tttcagccca atttatataa gcttgaattg gagacgatag
     6061 acgaagaaaa aagagtaacg gagtatattg actattcctt cggttttcgt aaaatcgaaa
     6121 tcaaagataa aatcatctac ttcaatggaa aacggatgat tatcaacggg gtaaatcgac
     6181 atgagtggca tccgaaaaaa ggacgcgcca tcactataga agatatggaa aaagatttgg
     6241 ctatcataaa aaatgcaaag atcaacggcg tacggacatc tcattacccc aaccagatca
     6301 cttggtatta tttatgcgac caagcaggaa tccatgtgat ggcggaaacg aacctcgaat
     6361 cccatggctc atggcaaaaa atgggcgcat ttgagccttc ttacaacgta ccaggtgcag
     6421 tacctcagtg gcaggcggct gttcttgatc gagcaaaaac gaatttcgaa acattaaaaa
     6481 accatccaag tattcttttt tggtctcttg gaaatgaatc ttatgcggaa gaaaacattc
     6541 gacaaatgaa cgattacttc aaaaaagtag accccgatcg acttgtccat tatgaaggga
     6601 attttccaaa tccagcattc gaagattcga tttcagatgt aaaaagctgc atgtatgcgc
     6661 caccagaaga gatcattgac taccttgaaa ataaagcgga aaaaccgttc atcttatgtg
     6721 aatatatgca tgacatgggg aattcgttag gtggaatgga ttcttacatg acgttgcttg
     6781 atcgttatga aatgtaccaa ggaggctata tatgggattt taaagatcag gcgatccaag
     6841 tgactgatcc aatcacaggc agagaagtga tgcgctatgg cggtgacttt gacgataaac
     6901 caagtgacta tgaattctca gggaatggga tcgtttttgc agatggacaa gaaaaacccg
     6961 ccatgcagga ggtaagatat tattatgaaa aatacagtaa ataaaagtca tatggatacg
     7021 gaaaaagttg caatcgtctt cggcgactgt acattaggtg tcaaatcggg gaatacgcat
     7081 tatatttttt cttatacaag aggcggactg gaatcgctca ataaaaacgg aaaagaatgg
     7141 ctataccggg aaacacttcc agttttttgg cgagcaacga ccgataatga cagaggtagc
     7201 ggatttggat ttaaatccgg tatgtggtta ggagccggat tgtatccaaa agtcaaagaa
     7261 atagaagtac tggtggacaa taaaaagatc gatttgccga ttgctccagt aaataatcaa
     7321 tattcaagta atgaatacgc tgaaacggtt gaaatcattt ttacactgga aatcaacaca
     7381 gtaccaaaaa cccaagtgac aatttcctac caaatcgctg gagagggtaa aatgcttgtc
     7441 aatatgcatt attatggaca aaaaggatta ccggaattac ccgcattggg tttaaggttc
     7501 attatgccaa cgaaagcaaa attttttgag tatgaaggcc tatcagggga aacatatccg
     7561 gatcggatgg ctggtggtgt cgaaggtatt tataaagtgg caggactccc cgtttcccca
     7621 taccttgttc cccaagaatg cggcatgcat atggaaacga aatgggtaga actaacaaga
     7681 aatcaaacgt tggataatac ggataaagat caagaagatt tttcgttgcg atttgaacag
     7741 caggatacgc catttgcttt ttccgcttta ccattcaaag cagaagaact ggaacaggca
     7801 acacatatag aagaactacc gccagaaaga cgaacggttc ttacgatttt tgcaaaagta
     7861 agaggagtcg ggggtatcaa tagctgggga gcggatgttg agtcacctta ccgaatcagc
     7921 agcgaagagg accatgaaat cggctttatt attcagtagc aatctttaga ttattatttg
     7981 aaaaacggag tttacaagac aaagctgctt tat
//
LOCUS       DAZUKC010000106         7960 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000106 DAZUKC010000000
VERSION     DAZUKC010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.108"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            24..1178
                     /locus_tag="ACG80T_002438"
     CDS             24..1178
                     /locus_tag="ACG80T_002438"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFS4842056.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1200..1952)
                     /locus_tag="ACG80T_002439"
     CDS             complement(1200..1952)
                     /locus_tag="ACG80T_002439"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4842057.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2316..4982)
                     /locus_tag="ACG80T_002440"
     CDS             complement(2316..4982)
                     /locus_tag="ACG80T_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS4842058.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNE
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5324..6775
                     /gene="cls"
                     /locus_tag="ACG80T_002441"
     CDS             5324..6775
                     /gene="cls"
                     /locus_tag="ACG80T_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFS4842059.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQKALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            complement(6980..7795)
                     /gene="thiD"
                     /locus_tag="ACG80T_002442"
     CDS             complement(6980..7795)
                     /gene="thiD"
                     /locus_tag="ACG80T_002442"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFS4842060.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKDAQQETVNDYVLLESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         2311 a         1664 c         1432 g         2553 t
ORIGIN      
        1 atcataagta aagaggtgtt gaaatgaaaa tagggattga tcgtctttcc ttttttattc
       61 ctaatttata tttagatatg acagagttgg cagaaagccg tggggatgat cctgcaaaat
      121 accatatagg gattggtcaa gaccaaatgg cagtcaatcg tgcaaatgaa gacatcatta
      181 cactaggagc aaatgctgcc agcaaaatcg taacagaaaa agaccgtgag ctaatcgaca
      241 tggtcatcgt cggaacagaa tccgggattg atcattcaaa agcgagtgcg gtaattatcc
      301 accatttact gaaaatccaa tcttttgctc gttcttttga agtaaaagag gcttgctacg
      361 gtggcaccgc agctttgcac atggcgaaag aatatgtcaa aaatcatcca gaacgaaaag
      421 tactagtcat agcaagtgat attgctcgtt acggcttggc aagcggtggt gaagtgacgc
      481 aaggtgtcgg tgctgttgcg atgatgatca ctcaaaaccc gcgtatttta tcgattgaag
      541 acgacagcgt atttctgaca gaagatatct atgatttctg gcgtccagat tatagcgaat
      601 ttcctgttgt tgatggtcct ttatctaatt ctacgtatat cgaatcattc caaaaagttt
      661 ggaatcgaca taaagaattg tcgggtcgag gactcgaaga ttatcaggcg attgctttcc
      721 atattccgta tactaagatg ggaaaaaagg cattgcaaag cgtattagac caaacagacg
      781 aagaaaatca ggaacgtctt atggctcgct atgaagaaag catccgttac agccgacgaa
      841 tcggtaatct ttacactggt tcattatacc tggggctaac ttctctactg gaaaattcga
      901 aatcactaca gccaggagat cgcatcggtc tattcagcta cggttctggt gcagtaagtg
      961 aattttttac aggttatctg gaagaaaact atcaagaata tctcttcgca caatctcatc
     1021 aagagatgct agattctcga acacggatca ctgttgacga atatgaaacg attttcagtg
     1081 agacgcttcc tgaacacgga gaatgtgcag aatatacttc agatgtacct ttttcgatta
     1141 caaagatcga aaatgatatt cgttactaca aaatataata aaatcataaa aagaaccctt
     1201 cacgagtcgt gaaaggttct ttttttatct gcggaccaaa ggagcagtct ataggtcagc
     1261 ttagtcgtac taaggatttc tcgtttttca ctgaatagtt tgcgaatgta cctggtcaca
     1321 acaggatctt ttgcgtacgc gtcatagtat agcttcatca attcttcagt aattgattcc
     1381 tgtgtaaaat aagtaaaatc ttttttcttc actttgaatc cttcatcaat taagtatgtt
     1441 tcaaattgat ccattacaac tggcgatgta ttaggatctt cttctatcat tcctagttgc
     1501 ttttcgatag gataaaaaac atcatcgatt gattccacta ccctgattat cacgcaccat
     1561 ttgcttgact ttttcatcaa tttttctaat tgaataagat gtaccaatgc tggatttgct
     1621 gctgtaaaaa ccatttcata tgtctcattt gattggataa attcttctaa cgactgtttg
     1681 ataaaggaaa catttggcaa ctcatttttt tctgcttctt tttttgcatt ttctagcatt
     1741 cctgaagaaa aatcgatcaa atcatagcga ttaatttctt ttgctaagga aagaaatctg
     1801 ccagatcctc cagcaacgtc tgctgctgtt tcagcaggca ggactttctc cgttttcaga
     1861 taatttttta catcttcagc aatagttact tgcgattctt gctggatttc ataatattct
     1921 tcagcaaatt catcccattc tttttcattc atttgatttc ctctaatttt ttgttatatg
     1981 ttttcacttt tcggaataaa ttattctcct cttttatcta ttctatctct tttgatgaat
     2041 aataaaatga ttggctttcc gtctatcata aataaaaaaa gggactgtga ccaaagtcgt
     2101 gtcaaagtcc ctctttccga ataaacagtg tccaatcaga tgaccctcag tattagctca
     2161 aaatgctgat tttacaactt cttccttaaa cggtgttctc ctgccaactc ttcggtcaca
     2221 agtcacaata ttcaacaaac atgagaagct gtttgttgaa attgcacctt gtgactcaga
     2281 attaaacggc aggctcacaa cctcgttgat tcgttttatt tatttccttg tactcgtaca
     2341 atgatcaatt tagtgatttc catcaaaaca attgtcacaa gtgcgattcc catacatgta
     2401 aagacttctg tcaatccaaa tgctgatggg atagagaaga cttctttcag tcctgggatc
     2461 attgttaagc agtaaagcgc gctacataca actacagcta gtaagacact tctgttagag
     2521 aagaaccctg cttggatagc tgtttgtgtg tttgaacgtg ctgggaatgt ttgcaatgtc
     2581 cgacaccaaa tcaatgttga aaatgacatg gctacaccga attcaggtga atgctgcatg
     2641 ccaagccatt gagcaaagat aacagcaatc ccaatcagca caccacgata cgttacagaa
     2701 atcaatgttt tgtccgtaaa gattcctgaa tttggatctc ttggcgggcg gttcatcgta
     2761 gcaggttcag ctttttctgt acctaatgca attgctggca gtgaatcatt gactaaattg
     2821 atgaacaaca gttgtaatgc tgtaaatgga ttggaccagt cagcaactaa ggcaaagatg
     2881 atcgcaatga tcgcacctaa atttcctgca aatagataag aaatagcttt cttgatatta
     2941 tcaaacacat tacgtcctac ttctactgcg ctgacaatag aagcaaagtt atcatccgtc
     3001 aataccatcg ctgctgcatc ttttgccaca tctgtacccg ttcccatagc aattccgatg
     3061 tctgcttgtt tcaatgctgg tgcgtcattt acaccatcac ctgtcatagc tgagatatga
     3121 cctttatttt gccaagcacg aacgatacga attttattct caggtgaaac acgtgcataa
     3181 acagtgactt tttctaaaac ttcgtttagt tgatcttctg tcaattcatc tagttccgtc
     3241 ccggtcaaag ccaggtttcc ttcttcaaaa atcccgattt gttttgcgat agcaacagct
     3301 gttgttttgt ggtcaccagt aatcatgatt gttttgattc ctgcacttct tgcttctgtt
     3361 accgcctgag tcacttcagc acgtggagga tcgatcatag caagcaaacc aacaaggatc
     3421 aaatcgtctt catcttcaaa tgataaatca aaattatcaa gtggacggta agcaaaagct
     3481 agtacacgta atgcgcgtgt tgaaaaagct tcattttgct cttggaattt tttcatcaat
     3541 tcatccgtca tcggaacgac ttcaccattt agcaatactt ttttactgcg ttggaagacg
     3601 atatctggtc cgcctttgac aaacaagtaa ttttcgttat cgatcgtgtg tccagtagac
     3661 attaattttc gatcagaatc gaaaggaagt tctgcttgtc ttgggtagtt gtttcggatt
     3721 tcctgataag gctggtttaa tttttcacta aagtcgataa acgctacttc cgtagggtcg
     3781 cccaatttag taccgtcttc actgatcgat gcatcattcg ctaaaacact gatttcgatc
     3841 aagcgacgtt cgtcagctgt ccattttgaa gggtcatttg tgaaatcccc agtatcgcca
     3901 ttacctaaaa agtagtcaac aactgtcatt ttgttttgtg tcaatgtacc agtcttatct
     3961 gtacagatga tacttgtcga accaagtgtt tcaactgctg gaagcttccg aataatggca
     4021 tgacgttttg ccattttatt cgttcccaaa gataacacga ttgttacgat agactgtaat
     4081 gcttctggaa tagcagctac ggctacggct acggcaaaca tcactgcgct gacgatatct
     4141 gcagtcatat ctcctgtacc ttctccaaga taaatacgaa acagttggat acctaaaatc
     4201 aataaagaca atgccaaaat agccagactt agttttttac tgaatttatc cagacctcgt
     4261 tgtaacggag tgacttgctc agttgttgat tccaaaaggc cagcaacttg tccgatctcc
     4321 gtattattcc cagttgaagt cactaagaat ttaccgcgtc cgtaagttac gatcgttcca
     4381 ctgaatacca tatttgtacg atctccgatt ggtaccgtac ttgtgatatc cgttacttct
     4441 ttttctgcag aagtcgactc tcctgtcagc ataccttcat ctacttttaa tgatcccgca
     4501 tctagtaaac gtccatcagc aggtacataa tcgcctgcgt ctaacaacac gatatctcct
     4561 accacaagtt cgtttgccgg aatcgttttt tccttaccat cacgaatcac tcgagcatcg
     4621 ggcgctgaaa ggtttttcaa cgcttctaaa gaaccttctg ctttttttgt ttggacaaca
     4681 ctgattatag aattcaacat caatactgca aaaataacta agaattcgat gtagtcaccc
     4741 atgaccatct gaatcagtgc gacaatcagc aatacgataa ccattgcatc tttgaaagtt
     4801 tctaagaaca atttccaagt tggcttcttc ggcttttctt ttagctggtt caagccatct
     4861 ctttctaatt tttccttcgc ttgcgtggtg ctcaatccat cggttgagct ctgcgtttgc
     4921 tgcatcaacg agtcgatcgt ttctttatag accgcttgtt gtgcttgttc cttgttttcc
     4981 aaatttcatc ccccattttt tgatcttggc cctagtcctg tctgaaaaaa aaagagacct
     5041 aagacaaata cgcatttgtc ttaggtctca ccatttcata caataccaga aaaccatttg
     5101 gttaacttac tgttgatatt atcgcaacaa acgttgccgg ttactcccct aaggagatat
     5161 tcaactgtac taatatccta ccttattaac ctacaaaaag caactacttt agcccgattt
     5221 acctacattt tcatgaaagg agtgaaaagt atattcacct tggtaggctt ctttcttcta
     5281 tcatgctata ctaatgttat tgtatgtaag gaggaacatt tttgtggtat ctagtattat
     5341 aaccgccctt tatctattaa atgcacttat tgctttggtg gctattttga tcaaaccccg
     5401 agatgtagca gccatttggg catggctctt agtattgttt gcccttcctg gggtgggctt
     5461 tgtattatat ttatttttcg gacgtggatt aacggataag aaaaaattct atctccgaca
     5521 aagtgacttg aaagaattag aaaactttca gtcttttaga ggagatacca ttgaacatta
     5581 cgatcctgac atgggcgata aagacaaaca gcaatttgtc gacttcttct cttcattaaa
     5641 tcgtatgccg ctgacaagaa tgaattctgt cactcttctc acagacggac aagagaaatt
     5701 ggattcactg cttcaagatc taaaaaaagc caaacattcg atccatatcg aatattacgc
     5761 atttgtgaca gataatatcg gccagcaagt cttacatgtt ttagaagaaa aagccgcaga
     5821 aggcgtggaa gttcgaatat tatatgatgc atttggctct catggtacaa aagcaaaaga
     5881 tttcaatcgt ctaatcaaaa atggtggaca tgtccataca tttgttacct cacaaaaggc
     5941 attacttcgt ttccgattga attaccatga tcaccgaaaa atcgttgtga tcgatggaaa
     6001 gattagttat accggtggtt tcaatattgc caatcaatat gtaaatacaa caaaaaaatt
     6061 cggctattgg cgcgatacgc atatacggat tttcggtgcc gcttcttctt tgctccagct
     6121 tcgcttctta acagactgga acgtctcggt acctgaagaa aaaaaggtcg gctatcattt
     6181 gaattatttc tttaaaaaag cagatcgaga tgaatctaag cttgctgata catccatcca
     6241 gcttgtttca agcggaccga ataacgaaag ggaacaaatc aagctttcat ttatcaaatt
     6301 gattacttct gctaaaaaac gtgtttggat acagacacct taccttgttc ctgatgaaag
     6361 tgtcattgct gctttaaaaa tcgcaactgc ctctggtgta gatgtgaaaa tcatgattcc
     6421 caacaaaccg gatcatcctt ttatttatcg agcaacacaa tattatgctc ggcagctgat
     6481 caaggaaaat gtacaaatcc ttgtctatga gaacggcttc ctccatgcaa aaacattgat
     6541 aatggatgat gaaatctgca tggtaggttc agcaaatcaa gatattcgta gctaccgatt
     6601 gaattttgaa acaagtgctg tcatttacga tcctgagttt ttagaagaac ttgctactca
     6661 gttcaaagaa gatgagacac attgttcatc catgacaact gaaacagtca aggaaatgtc
     6721 taactggcta ttattcaagc aacaaatttc tcgattattt tctccaatcc tataattttt
     6781 ttattcaagt agagaaaaat atactaagat taaagggact gtgacaaaaa tcttgtcaca
     6841 gtcccttctt ccgaacaaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat
     6901 tcaacaaaca tgagaagctg ttttttgaac ttgttccttg tgactcggag ctggacggca
     6961 ggctcacgac ctctttcttt tactctaatt caccagctgc ccaatgattg atgggtccat
     7021 atttgtgccc tacatttatt tcttcacgaa ttgcacggtt tacatagtct tttgccaatc
     7081 ggatcgcttg ttcaactgtt tttcctttcg ctaactcagc tgcaatacat gcagatagcg
     7141 aatcgccagt tccgttgatc cggtcggtag gataaaatgg tgcatttaac caaaatgatt
     7201 ttcctgattc gagcaatacg tagtcattga ctgtttcttg ctgcgcatct ttagcatgat
     7261 gtcctttgat cataacgttt tttgcaccca tatcttgcag ttcttttgct gcttgcacaa
     7321 aatcttcttc agctgctaat gtccgtccag tcagcttttc tgcttcataa aaattaggtg
     7381 tcagtacttc ggccaaaggg acaagctttt ctttcaagct ttgcaaagca gattcttcta
     7441 gtaaaagatt tccatgtttc gttacgatta cgggatccac gactaatggt ccaaaatcat
     7501 atttttggta attttttaca actgtctcaa tcaactttga atctgctagc atacctgttt
     7561 tagctgcacg tatttgaaag tccttcgcaa tcagttcaaa ttctttgtcg ataaaatctg
     7621 ttggcaatgt cacacttgct ccgattccgt aagaattacc tgcaacacat gcggtcatga
     7681 cagaaacacc gtaaacattt cttgtaaaga atgtatgcat atctgcttgc atccctgcgc
     7741 ttccatcgct atctgatcct gcgatcgtca ttgcttgtac atactgatct tccatcaaat
     7801 cgattcctcc atttttataa aaagattttt cataatgctg ttactgtttc tatcatatgt
     7861 ttttcaaagt atccctcagt aaatatacca ctaccttttc ttggtaatca aatttatttg
     7921 aaaaaacatt tgtaataaat cagaaaatag ttgtcatttg
//
LOCUS       DAZUKC010000107         7760 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000107 DAZUKC010000000
VERSION     DAZUKC010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.109"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(552..1898)
                     /gene="gor"
                     /locus_tag="ACG80T_002443"
     CDS             complement(552..1898)
                     /gene="gor"
                     /locus_tag="ACG80T_002443"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFS4842061.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWKASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFCGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2032..3285)
                     /locus_tag="ACG80T_002444"
     CDS             complement(2032..3285)
                     /locus_tag="ACG80T_002444"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFS4842062.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(3291..4214)
                     /locus_tag="ACG80T_002445"
     CDS             complement(3291..4214)
                     /locus_tag="ACG80T_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFS4842063.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4435..6927)
                     /locus_tag="ACG80T_002446"
     CDS             complement(4435..6927)
                     /locus_tag="ACG80T_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFS4842064.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6934..7401)
                     /locus_tag="ACG80T_002447"
     CDS             complement(6934..7401)
                     /locus_tag="ACG80T_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFS4842065.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(7537..7610)
                     /locus_tag="ACG80T_002448"
     tRNA            complement(7537..7610)
                     /locus_tag="ACG80T_002448"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7574..7576),aa:Arg,seq:acg)
     gene            complement(7668..7741)
                     /locus_tag="ACG80T_002449"
     tRNA            complement(7668..7741)
                     /locus_tag="ACG80T_002449"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7705..7707),aa:Pro,seq:tgg)
BASE COUNT         2162 a         1691 c         1245 g         2662 t
ORIGIN      
        1 aaatcagtgt tttgtcctga gtattagaga aacaaatgga aaaatagttc ctcatatttt
       61 gccgtttttt gagctaatac cgaaggacag ctgattgaac actatttgtt cggaaaaagt
      121 gactaactat gacacaattt ttttcacagt cccttttttc acttgatttg attcgtacca
      181 gcgaacttga cgagaacttt cttggtttgg taactatatg gtattctttt aacatcctcg
      241 tttgcttctt ctaatttatt tatacaaagt cacacttttt taaacatctg ttcttcgata
      301 catttctatt tcttttaaac atcgatacca atattttgaa cttaagagtg cataccaaat
      361 ggattcattt actcttcgtc agttacgaag ataatcaaaa tctccaactt ccctacttat
      421 ttcgttttga gaattatcga agcaatctta cataaataac taaaaaacaa aaaaccagaa
      481 aatcagtata ctgattttct ggttttacat ggtttgttca ataaaaagct gttggaactg
      541 ttaatattaa attatcgcat cgttacgaat tcttccgctc ctgttggatg gatggctact
      601 gtattgtcaa agtcttcttt agttgcaccc atcttgatag ccacggcaaa accttgcagc
      661 atctcatcga caccaacacc aatagcatgt aagccgacga ttctttcttc ttttccgaca
      721 cagatcaatt tcatttcgca tttttggcga taatcattta atgcaaagta cattggggta
      781 aagcgtgaac gataaacttt caattcattt tcgccatatt tttccaatgc ttcttcttct
      841 gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga ctgtcggaac aagtttatag
      901 tcaagataag aatctttctt tccattaaat aaacgttctg ataaacgtct accagctgcg
      961 attgcaacag gagtcagatc taatttaccg atcacatcac caacagcaaa aatatgttca
     1021 tccgttgtat tttggtattt atctactttg ataaaccctt ttttgtccaa ttccacattt
     1081 gtattttcca gacctaattt ttcggtattc ggtacacgtc caccggcaaa aagtacacaa
     1141 tcaccagtta aggtttcacc attttcaaat agcaccgtat attcgtcatt gtttttagta
     1201 atttctttag caatatgatg tgcgtaagtt tttattccag cttcttggta catttggatc
     1261 aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc gttcttttcg atatgcccac
     1321 atgacttcac tacctaaacc acaaaataca cctgaaagtt cagccgcaat gtatccagca
     1381 cctaaaacga ttgctctctt aggtaactct gttaattcaa aaaatccatt tgaatcaatt
     1441 gcgtattctc caccaggaat ggtcatttta cttgcacggc ctccagtagc gatcaagatt
     1501 ttaggagcac gataggtttc accgtttact tcgatcgttt gactatcgat aaaagtagcg
     1561 taacctttta tagcctcgac atgattgcta tctaatcctc tttgataagc accgtgtaga
     1621 aaatcaatat atttttctcg tctttctact agaccggcga aatcaacatt ttttaaatct
     1681 gcttggatac cgtaacttga ggcatctcgt tcaattgttt ccaaaatcgt acttgctttc
     1741 cacataactt ttttaggtac acaaccgacg ttgacacatg ttccgcccaa ttcattcgct
     1801 tcaatcagca aaacttttgc gccatgcatc cctgctctgt ttgctgaagc aataccgcca
     1861 gagccgccac caattacgat gtaatcatat atttgcattt tgttcctcct tattgaagag
     1921 atcaatcaaa acctttattt aacgatcctt tttctacaga aaacaagtgt aacacttttc
     1981 ttgaattttg tatgcgaaga tgcttaaaac agggatatat cagctttttc cttatttacg
     2041 ttttcggatc atatctccag gtttcaccgg ctgtgcgact ggcatagtta aaatcatcat
     2101 tggatttggt gctcggtcaa tggattcgcc ttcttcatta tgcataactt caacggtttg
     2161 actgaaatga ctgaatcctg gaccataaaa ttcgatttca tctcctacag aaaacaagtt
     2221 tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa gataatactt caccgacaaa
     2281 tttatatact gggattttac gacgttcacc gaataattgt tcgttttcag ttggtgtatt
     2341 gtagtaaaaa cctgtagcta attctcgttg tgctactttc cataactcgt cgatccattc
     2401 ttgcttgcag acatagtttt caggatcttc catataactg tctacagccg ctttatagac
     2461 atttgacacc gtagatacat aatggatcga tttcattctt ccttcgatct tgaagctgtc
     2521 tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt gacatatcca ctgcactcat
     2581 cgagaattct tcttccactt cgcctgtatc agttaaactg tttcgttctt gaccaaaagg
     2641 catatcgaac aatccatact tccagcgaca agattgagaa catccgccac ggtttgcatc
     2701 ccgcatcgac atatggttgg acaatgtgca tcgaccagaa taagagatac acatggcacc
     2761 atggataaat gcttcgattt cggtatccgt atttttacga atctctgcca cttcctccat
     2821 tgaaacttca cgagctaaaa cgacacgttc caacccttct tctttccaga attccaatgt
     2881 ctcaaaatta gttgctgaag cttgagtgga taaatggatc ggcagacctg gggcttctgt
     2941 cgcacaaatt tcgatcaaag ctggatcaga aacaatcact gcagaaatac cgacatcacg
     3001 caaactgcgg aaaaattccc ctgctccatc ttcatttcct tcatgggtga ccatattagc
     3061 agcaacgtat accttcgcat catgttcttt tgcaaaagca actccttcag ccatttcttc
     3121 ataagtaaaa ttacctgcac ggcttcttaa accatatgca ttcccgccaa tatatactgc
     3181 atctgctcca taatggatcg cggtttttaa cttttccagt gttcctgctg gcgctaggat
     3241 ttctggtcgt ttcaattttc tttttactgt catttctttc atcatactcc ttatttgata
     3301 gcagaaggat caatataaaa gaatcctgta tctaattcac ggttttcagg atgcaattta
     3361 ctgatttcgc tagtcatttt ttccgctttt tcttgatccc agctaccttc taaaatttgt
     3421 ttttttgctt cggcgaatag tcgagtgatg tttacgaatt tttcgcctgg agtaaataat
     3481 ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt cattcaattc attcgagaga
     3541 ttcagatcat tgctggcaaa aatatgtgtg ccatgactat cttcataaat agaataatgt
     3601 gtctcttctt ttttaggttc cgagataaaa agacctcttt cacgatcttt ttgttcgtct
     3661 tgtttcgtat agttatagta attctgtagc agtggacgtt tggattgatg gatgcatgta
     3721 gcaccataca caagggtttc tacaggaata tcgagttttt cttccatcgc cttcatctct
     3781 tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc cttttttcgc ccagaaattg
     3841 atttgacgtg cgctcgttac taatgtttca gcatcataaa tatacggtat acgcaattca
     3901 ggattttttt tcatcgtata aataatacct gtatcaccaa ctgtgatcac atctacgttt
     3961 atttctttta aaaaagccaa atattcaggt acatttttca ttttttcagg atgaatgatc
     4021 ccatttacag caatattcgc ttttttacca tacttgtgtg ctaattctac caattgtcgc
     4081 tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa attgctcttc accgaaatat
     4141 agtgtatctg cacctgcagc aagcaactgt tctgcttgtt caatagtttc tactgtggtt
     4201 atgagttcga tcataatttt ctcctcctac aacttcataa tagtagcata gctctaaaaa
     4261 ctttccaata cttcttctat ttaaagtcgg atttttgaaa attatttcac aaaaaatcaa
     4321 aataaagttc gatatattct gtccaaatat aaaaagagat tgtgacaaaa atcttgtcac
     4381 aacctcttaa tcaagtctta ctattataaa aattcagcta cacaggccaa gtaactatag
     4441 ggaacctact gtttcctttt taggtccgcg aacattcaat gtgatcttac cttttgaagc
     4501 acctactgtt acgtggtcac ctaaacgaat ttctccgcca agcaaagcct cgctcaatcg
     4561 atcttcaatt tctttttgaa gtgcgcgacg gattggacgt gcaccatatt caggatcaaa
     4621 tccagctttc ccgatcacat cgattgcaga tggtgtgatt ttcacttgaa tatcttgttc
     4681 tttcaatcgt ttgataatcg ctttgctcat gatttggacg attgtatgga tttcatcttt
     4741 actcaatgaa tggaagacga cagtctcatc gattcgattc aagaattcag gtcggaaagc
     4801 tttctttaac tcttctaaga tacgtttttg catggctttg tgatctttag taacatcttg
     4861 gacattgaat ccgacatttt tttcttctcg aatttgtgtc gctccgatat ttgaagtcat
     4921 aatcatgatt gtatttctaa agtccacttt tctaccttta gaatctgtta aatgaccatc
     4981 atccaatact tgcagaagta aattgaaaac gtctggatgt gctttctcca cttcatctaa
     5041 gagaatcact gaatatggtt tttgacgaat tttttctgtc aactgtccac cttcatcata
     5101 ccctacgtat cctggaggag acccgattaa tcggcttgta ctgtattttt ccataaattc
     5161 tgacatatca acacggatca gggcatcttc acttccaaac atgacttctg aaagtgcttt
     5221 ggccaattct gtttttccga caccagttgg tccaaggaac atgaatgaac caattggacg
     5281 atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca cgtgaaactg ctttgacagc
     5341 ctcatcttga ccaacgacac gttcatgcag aagtccttcc agttctaaca agcgttcgct
     5401 ttcttttttc tccaactgct gtagagggac accagtccac tccgatacaa ctgtagcaac
     5461 atcttcttct gttacacgat cagaataacc ttgctcctgt tttgcttcaa ttgcagaaac
     5521 tttttgcagc tttttacgta gtgctttttc ttgtctgcgt aattgagcag cattttcaaa
     5581 gtcttgtttt tggatcgctg cttccttttc ttgtactagt tcatcgattt ctagtttgat
     5641 cacagttgat tttgttaagt gatcggtttg atccaaacgg actttcgctg ctgattcatc
     5701 gatcaaatca attgctttat cgggtagttg acgagaagtg atataacgaa cagaaagatt
     5761 gactgctgct cgtacagctt catctgtgat ctccactcca tggtgctctt catatcttgt
     5821 acgtaatcct tgaagaatca cttctgcttc ttcaggagtt ggctcatcta cttgaatgcg
     5881 agcaaaacgt cgctctaatg cagaatcttt ttcgatatat ttttgatatt catctagggt
     5941 cgttgctccg atcgtttgta attctcctcg agctaacgcc ggtttcaaga tattcgaagc
     6001 atcaattgct ccttctgctc cacctgcacc aattaaggta tgcaattcat caatgaataa
     6061 gattacttga ccatcattat agatttcgtc gatgaccttt ttcattcgat cttcaaattc
     6121 accgcgatat ttcgtaccag caaccaaaga tcccatatca agcatcatca ggcgcttttc
     6181 ttgcatgtct tctggcactt cacctaaaat gattttttga gctaaccctt cagcaatggc
     6241 tgttttgcct acaccaggtt cacccaccag tacagggtta ttctttgtcc gtcggctaag
     6301 aatctggata agccgtttca cttctctcga acgaccaaca actgggtcca gcttgttttc
     6361 ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc gtaggtgttc cttgcggtgt
     6421 ttgccgttta tcttgtacat ttacatttct acgacgattc attccttttc cagcagaatc
     6481 accagctagc cccatttttt gtttcagaac ttgacgggct tttgaaaggc tgatacctaa
     6541 gttgatcatg attcgagtag ccatgatact ttcatctttt aataagctca tcaaaatatg
     6601 ttctgttcca actttttgag aaccagtttt cttcacttcg ctgctagcta aagcaaaaac
     6661 ttgtttcatt cttggtgaat atggaagaaa gactccttta gggtaagaac ggataccacc
     6721 ataaccaatc agatgttcaa tttcttcata aatttccgtt tcattcaaat ccatttcacg
     6781 taatactttt ccagcaattc cattttgctc gaccactaag gctagtaaaa catgttctga
     6841 actaactgat tgatgtttca accgttttgc ttcttcttgt gcaatttcta atactgcttg
     6901 ggctctttca gtaaaaagtt cttccatcta atctcacttc ccatcttcat aacttaaacg
     6961 ttccagaaat gctcggagaa tccgagcacg tgtatgatct tcatattcat tgaaattcaa
     7021 tgtgtttttt gcaattgcac tcaacatcaa gtttccttct ttttttgtaa ttatttggtc
     7081 ttcataaagt ttttgaataa tagcaaaagc atttttctca ctgattgtat catcaaataa
     7141 ctgatttatc tgctcaagta attgtttttt atctgaaatc cttacttttg caattcgtat
     7201 ataaccacca ccaccacgtt tgctttctac tagataacca cgttggatgg taaaacgggt
     7261 attaatcaca tagttgattt gtgaaggaac acagttaaag aggtctgcca tttcaatcct
     7321 tcgaatctcg atcatttcac tattttccag aattttcttt atatatgatt caattaaatc
     7381 cgaggtattc tgatgtgcca tcttattcat tccctttgac taatattgac cttaattata
     7441 acgaatttga taatagaaag aaagaagaac gctttttgtt aaataaatcc tttagaatca
     7501 acaaaaaaaa atacggaata tactattccg tatcgacgcg cccaagagga gtcgaacccc
     7561 taaccttttg atccgtagtc aaacactcta tccagttgag ctatgggcgc atattcaatt
     7621 taaaaaaatt ataaatcatt tgaaaacaaa aagcaagata aaaaaaatcg ggaagacagg
     7681 attcgaacct gcgacccctt ggtcccaaac caagtgctct accaagctga gctacttccc
     7741 gtttaaaaaa taaaaaagac
//
LOCUS       DAZUKC010000108         7487 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000108 DAZUKC010000000
VERSION     DAZUKC010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.110"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            148..1539
                     /locus_tag="ACG80T_002450"
     CDS             148..1539
                     /locus_tag="ACG80T_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4842066.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(1612..2259)
                     /locus_tag="ACG80T_002451"
     CDS             complement(1612..2259)
                     /locus_tag="ACG80T_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4842067.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(2267..2953)
                     /locus_tag="ACG80T_002452"
     CDS             complement(2267..2953)
                     /locus_tag="ACG80T_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4842068.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(2959..4032)
                     /locus_tag="ACG80T_002453"
     CDS             complement(2959..4032)
                     /locus_tag="ACG80T_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4842069.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            4254..4928
                     /locus_tag="ACG80T_002454"
     CDS             4254..4928
                     /locus_tag="ACG80T_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4842070.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            4933..6273
                     /locus_tag="ACG80T_002455"
     CDS             4933..6273
                     /locus_tag="ACG80T_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFS4842071.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(6422..7408)
                     /locus_tag="ACG80T_002456"
     CDS             complement(6422..7408)
                     /locus_tag="ACG80T_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFS4842072.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         2320 a         1387 c         1322 g         2458 t
ORIGIN      
        1 agggcgctaa agggaatcac accaattttc ataccaattg gtttcattta tgaagttgat
       61 tccttcgatt aatgagcgtt tcattgaaag aaaagggttg agggagaaaa acatatgatc
      121 aggttgttgg aaggaaacta tagaaagatg gaaaaaatca ttcgaattct gtccatttct
      181 gacaagtgga tgactaaaaa agaacttgcc aaacgaattg gtagttcaga atctacattt
      241 gtaaggtata tcgaagagat caaaatccgc tggggaacgg atgtaacgat tcaatcttca
      301 caaaaattgg ggtatcgtct tgaacatttg aatgtaccag cttatttaaa tgttctgatg
      361 gaaatgtcgc aaagttctac aaacagtcgt ttattaaatg agattattgt caatccgggt
      421 aaaacgattg agtattattg tgaaaaaatt tccatcagcc ggtcttcttt tacacgtaag
      481 ttgaaacaat gcaatcaaat tttagaaagc tatttattaa aagtgatcgt tgaccaaggt
      541 tttcagttaa ctagccttaa aagtgagctc cagttgcgta tctttgttac ctttttcttt
      601 ttaagttact acggaagacg cacgttgcca tttccaatcg aaaaaaagaa gatcaaagaa
      661 attatggtca agaataattg tcagatgaat ttgtttcagg aagaaagccc atatgagcaa
      721 tctttcttta ttatgtattt tgttgtgcat ctcatgagag aatcccaagg aactccattt
      781 gaatggaaag ctggagactc ttatgtacag ttgataaata tcgataaaaa agacttctta
      841 atcttgaaaa atctcttagg aaaattgaag tacagcagct atataaaagt agtgaactat
      901 tttgtcaata cgattttttc agaattttcg gagaaagaaa aaaatgaaat cagtgagcag
      961 atacgaaaaa atatgtccta ctgttcgttt tcatccgctg attgcaaaga aaaaaacgaa
     1021 gaatatgttg ttcaattatt aacgcaaatt tattttttct cccgtatttt cccatacgat
     1081 acgactcatt tgacgatccg tgcaacttac tttgcacagc agatgcaaca ccgccagata
     1141 cgtctatatg ctggatttcg tgcagaactt caattttatt ccactctact gaatcagaat
     1201 ctgatttatt atttatctag ctatatcttt tggatgctta tcggtaatcc tgaaattcac
     1261 cattatactt ctgataaaaa aatactgatt atcagcgact taagtcttcg acatagtcaa
     1321 tatattgaag aatatatcag tgatattctt gctgtgcata aaattcactc tgagacaact
     1381 gctattactg aagatcagct gtcgaaatac aatcttctcg agtatgatct gatcgttacg
     1441 aatcaaccca ttttgaatgc ccatgttcct catatattga ttgatgatag tgtgtcattc
     1501 gcaaatgaag aagaactcat ccgcttattc gagttatgaa taatcaaaaa acagctatgt
     1561 ataaaccacc ctgataaaaa tttcaggaca aatacacata gctgtttctt tttattgtat
     1621 tccttcagca atccaacgag atttatagcg aaagtctttt aacaactctt cttttgtcca
     1681 attatttgca atgagatccg tcaaagtgtt cattacgagg gtcatggaaa agtataggaa
     1741 actaactgct tcttccgacg attgaatgga gaaatgattg attcttaata attccagcca
     1801 tttttcgttc attggagaat cagccgatag ttctctacgt ttggttgtct gctgatcatt
     1861 tcgctgggtt aacaagtata acctttgcca atagatactt tcgtgatcta agtcgcagca
     1921 ccactctaaa tatttttcga tccccaaaaa gaaatgttgt ttgttttcgc taatgaattt
     1981 taagatatct tgatggacta tgtgagaact gcttttgatc gtatacaaat aagcatcttc
     2041 caaatcttga aaatatttat aaaaagcacc gcgcgacatc cccatcttgt caacgatttc
     2101 tgagactttg acttggctga tgtgctgtga gcaaaatatc tccagtaata ggtcatcgat
     2161 tctctgtttc tttgcgttcg ataaattgaa aaaagtttct tttggcatcc acatctctcc
     2221 tttatctctg attatacgtg gatagccctt cattgtcatg aaggattcat tccttctttt
     2281 tctcttcttc tgtcaagtac ccatcttcca ttcgataaac gcgatcactc cattgaatca
     2341 tacggacatc atgagtaacc ataatagtcg ctttttgttt ctcatgagct tctttaacta
     2401 gtaatttgac gacttcatat gcatgatctg tatccaaact agctgttggt tcgtctgcta
     2461 agatcaagct tggctcatta tataaagcgc gagcaattgc cactctctgt ctttctccac
     2521 cagaaaggtc tttaggataa ctatttttaa gtgactggat atctaaagat tgcagcaagg
     2581 aatcgatttt tgttgaatct tctttttttg aagtaacttg gtcaactagt tcaaattgtt
     2641 cctttaccgt taaaaaagga atcaaattag agctttggag aataaatcct atttctttaa
     2701 agcgtaaatc tgctcgtttt ttttctggaa gctcagttag tgagtgaccg ttgatcacta
     2761 tttctccgga agtcggtgtt tgcaatcctc ctgaaatggt aaggaaagta cttttacctg
     2821 aaccagaagg accaatgatg ctgacgaatt ctccttcctt tacataaaaa tcaattgctt
     2881 tcaaagctga gacctttgta tgaccagaac caaatgtttt ggtaactttt ttcatcgtta
     2941 atattttttt catttttatt aacctcctat tgctgtaatt gggtctactt tggttatcgt
     3001 acgtatcgaa aacaatccgc ctagcattgc gacaatgatc aaaatcccac tatatagcaa
     3061 ccattgtgac caaaaaacag caaaaggcat cgcgcttggt aaaatcagac tagtcaaata
     3121 agcaaacaaa agagcaagca gaacgcctac caccccaaca ataaaggact gtgctactaa
     3181 agatttagcg atgaagaaat ttttgatccc ttgtgctttc attactccaa aaatagcagt
     3241 cttctgaagt gtcatcacgt acatgaaaat accgaccacg gcagcaacca ctaagaacaa
     3301 aaagtatatc atcgcattta aggtcatatt ttgggcagaa tagccaggaa tactttcaat
     3361 gaatgtccca atagttaata actgtagacc cttcgatgct ttttctttcg aaattttagc
     3421 tgaatccttt attacgatcc cattgatcgg ctgttcttct tcagaagcaa acggctgttg
     3481 tccatacttc aaagcagtcc acgtatctag ggacgcatag atgacgggag aaactgtgta
     3541 ataagtttca ggaaagatac cgacaatcgt caaaggatcg tcgtaactgc caattttgat
     3601 cgtatctcca attttatagc ctgaatcagc cagattttga gagatgatga tttcattctt
     3661 tgttttaaat tcatgccctt tcgtgagttt tggtaataaa aatgcttctt tcgttgtgcc
     3721 gaaaactgta acattttctt tgttttttcc tttgattgct ccgctgtaca atccgattgg
     3781 tgctttctct cctccttcaa tgtatttcag atctcctctt gttaattgtg aagctgcaaa
     3841 tacttgattt gcatcctccg acaaaacaat ctcttgtgcg tcccaatcat cgattgcttt
     3901 tttaaactct tctgaaagtc cattcgctag tcctgacaac atgaatacga catacgcaat
     3961 caagaacata ataccgacga tcaagccata tcgtaattta gaatagcgaa tttctttgat
     4021 agctagaaac atttgttttt cctccctatg atagattgat agtgacaaaa tgtcacttgt
     4081 tgaatagtgt aactctaaag tgacaatttg tcacttgtgt tgtttttttc ttatcaataa
     4141 attgtctaag acatctttcg ataaaacctt aacatgagtc ataaaaaatt tatttaagcc
     4201 ttatttatgc tttccatgat aaactaaagt gaccaaatca atgaggtggg aaaatgatta
     4261 aactattagt agtagaagac gataaagtat tatccaataa tataaaagaa atactcgctg
     4321 caatcggcga gataacacaa gtttatgatg gtagtgaagg actgttcgag ttggaaagtg
     4381 gagtttatga tcttgcaatc ttggatttga tgatgccgga aatgaatgga tatgaagtgt
     4441 tagcaaaaat acggaaagaa aaaatacaga ctccagtatt gattttgact gcaaaagata
     4501 gtttggaaga taagattgaa ggatttgaac gaggtgcaga tgattatcta acaaaacctt
     4561 tctatcgaga agaactgatc atgcgcgtga aagcattatt gaaacgctcg ctaggtctct
     4621 ttgatgaaaa tgttctgaag tataaggata tcagttgtga tttaaaaagc aatgaagtct
     4681 tgtataaaga acaagttttg cccatacaag gaaaagagtt tgaattgctc gtttattttt
     4741 tacaaaataa gggaatcatt ttgacaaaag agcagatatt tgaccggatc tggggatttg
     4801 attctgatac gacgattaca gttattgaag tttatatgag caacttacga aaacatctgc
     4861 gaagtactgg attggataaa gaaatcaaaa ccttacgtaa tgtcggttat atactgcaaa
     4921 gggtgtgatg caatgaatgg agatattaca cgaaagcaac gtagtcgctt ttttgcgaca
     4981 aatgtagcag catttgcttt tatctttttt cttttgggat tcattacttt acagatatta
     5041 aaccaatctg catacagaga aacagatctg tcattagaga aaataaattt tgattctcga
     5101 acagtacaat tagagataca aagatatcag caaggcgatc cctttttaaa cccgactcaa
     5161 acggaacaaa acagaacaaa catggaacag ccattaatgg aaccatcaaa tgaccgtttt
     5221 aacactcaag tgatcttgtg gtctaaaaat ggtgagatat tgaataaagc agctattggt
     5281 gggcgtttca accaaatcaa agaattaaaa atggatacga aaaatctgaa taccatccaa
     5341 aatattgaat taaatgaaga cagtggctca tctgccctct ctttccgttc gattacgaaa
     5401 aaagcggaga cgaatcaaga tggcgttgcc tacatccaaa ttttagaaaa tacaaatcaa
     5461 atcaagaatt cattagcgac ttttcgcacg attatgattt tgtgtatgat tgttttttgg
     5521 attctctcta ttggtatcag ttattacttg tctagtctaa gcatgcgccc tattattctt
     5581 tcttggagaa gacaaaaaga atttgttgag aatgcttctc atgaattgcg cacaccgtta
     5641 acgattattc aaaatagttt ggaacatctt tttaccaagc ctaatcatac tattattgaa
     5701 gaatccgaaa gcatcgctca agcattgtca gagacgaggc gcctcactgg attgacttcg
     5761 gatttgctga caatcgctcg gaatgattct gatcagcagc tgctttcaaa gcaaatgatc
     5821 aatacacaag aatatatcga acaacttgtt aaaccatttc aagagatggc catcatagat
     5881 ggaaaagagt ttattctcca taacgatgca acattagaag taccaattga tgaaaaaaaa
     5941 atccatcaag ttttggttat tttgctggat aatgcattaa aatatacgaa tccagaagat
     6001 aagatcattt tggattcatc tgttaaccat aaagaatggc tgattcacgt aaagaatact
     6061 ggcccttcta tcagtgaaga agatcaagca aggatttttg atcgatttta tcgggaagat
     6121 caatctcggg cgaaagagac tggcggatac gggcttggct tggcaattgc aaaacaggta
     6181 atcgaacagc atcatggaca gatttctgta tccaattatc ttccacaagg agtcgatttt
     6241 aaagtccaat tgccgttgaa gcctaaaaaa taatgcttat ccctaaaaaa gggctgtgac
     6301 aaaaatcttg tcacagccct tttttgttgc gaataaacgg tgttccagaa gtagcttctt
     6361 cggaaataag ccgaaatttc tttaagctga ccacttctgt cacaacctct ttagcttacg
     6421 cttacgattt tttattgcgg tcttcaatgt gtacatcacc gcttgtgtca atatccaatt
     6481 cttctcggct aacatcttct gtcacttttt caacatcttc atgttcttct ttatgaatcc
     6541 caacttcttc acgaataact gtatgtttgc ttacatctac ttgctcttct ttgattggga
     6601 tagtaatcgt gtcatcttcc aatttttcgt ctgttccctg tgaagattga tctgttactg
     6661 gtttgcgttc gatgacgatt tcttcacgtt tgaccggtac ttctaccgtt tcagtatcgt
     6721 tgaccacatg tttgtggatg tcgacttctc cagttgtcac atcatgtgta tttacatcta
     6781 atttttcttc ttttagttga attgtatcat catctagatt tggtttgcga tcgatgtctt
     6841 cagtcttgct tgtacccatt gtatcagtct cgccaggtac gtccatcgga tcagttcctg
     6901 gtgtcatatt caatggatcc gttgtatttt gaaagcctgt cgtgccacct acgtccattg
     6961 gatctgttcc agtgccagtt tctaatccag tccccattgt gcttgctggg ctaacagtat
     7021 cttgtgtatc cattgtatct tctgttgttt ctggctgacg atagcctttt actgtgatga
     7081 caaattttcc atcagaaata tctcctctat attgatataa aggatcattt tctggtgtag
     7141 ccgtctcaga atcataggta tcaaaagaaa aagtatcttt tattttttcc cataaagaac
     7201 ggtcttcttc accatccatc cgattttcat ctgtttcaac atctatacca gacaatccgt
     7261 caaatcctag tctagatgct gcttcatcag tagtataaat aacgatatca tctcgttcat
     7321 acccttcgtt acgcaaccgt tcaattactg ataatgcttc ttccctcgta ctatagcttc
     7381 cgatgaccgt taaatttttc tcttccatgt aaattcctcc tagcaaatag atttatgatt
     7441 ggtatattgt tacttataaa tttattctat aggagacgat tgttttc
//
LOCUS       DAZUKC010000109         7484 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000109 DAZUKC010000000
VERSION     DAZUKC010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.111"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(94..1392)
                     /gene="eno"
                     /locus_tag="ACG80T_002457"
     CDS             complement(94..1392)
                     /gene="eno"
                     /locus_tag="ACG80T_002457"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFS4842073.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(1508..2263)
                     /gene="tpiA"
                     /locus_tag="ACG80T_002458"
     CDS             complement(1508..2263)
                     /gene="tpiA"
                     /locus_tag="ACG80T_002458"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFS4842074.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(2367..3557)
                     /gene="pgk"
                     /locus_tag="ACG80T_002459"
     CDS             complement(2367..3557)
                     /gene="pgk"
                     /locus_tag="ACG80T_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFS4842075.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(3667..4668)
                     /gene="gap"
                     /locus_tag="ACG80T_002460"
     CDS             complement(3667..4668)
                     /gene="gap"
                     /locus_tag="ACG80T_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFS4842076.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(4709..5800)
                     /locus_tag="ACG80T_002461"
     CDS             complement(4709..5800)
                     /locus_tag="ACG80T_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFS4842077.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(5949..7283)
                     /gene="rpoN"
                     /locus_tag="ACG80T_002462"
     CDS             complement(5949..7283)
                     /gene="rpoN"
                     /locus_tag="ACG80T_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFS4842078.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            7343..7414
                     /locus_tag="ACG80T_002463"
     tRNA            7343..7414
                     /locus_tag="ACG80T_002463"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7376..7378,aa:Arg,seq:ccg)
BASE COUNT         1936 a         1581 c         1384 g         2583 t
ORIGIN      
        1 atatttcagc aagtagagag ttcgattact taactaagtg atcatactcg actatctttc
       61 aatagtcgat catgtatctt cattagaaaa gaattatttg ttttttaagt tgtagaaaga
      121 tttcaaacct ttgtattctg caacttcacc aagttggtct tcgatacgta gtaattggtt
      181 gtatttagca atacggtcag tacgtgatag agaaccagtt ttgatttggc cagcgtttgt
      241 tgcaacagcg atatcagaga ttgttgaatc ttctgtttca ccagaacggt gagatacaac
      301 tgctgtgtag ccagcttctt tagccatttc gatagcttcg aaagtttctg ttaatgtacc
      361 gatttggttc actttgataa ggattgagtt agcaattcct ttttcgatac cttcagataa
      421 tttttgagtg tttgttacga acaagtcgtc accaactaat tgaactttgt cgcctaatac
      481 gtcagttaat ttcttgaatc cgtcccagtc gttttcgtct aatccatctt cgattgagat
      541 gattggatat ttagaaacta attcttcgta gaatttgatc atttcgtcag ttgttttttc
      601 gccttcgcct gagtcagcta gtacgtaaac acctttttct ttgtcgtaga attctgaaga
      661 agcagcatcc atagcaagaa caacgtcttt accaggtaca tagccagctt tttcgattgc
      721 ttcgatgatt acttcaaaac cttcttcgtt tgaaccaagg tttggagcaa atccaccttc
      781 gtcaccaaca gaagttgcta atccgcgagc tttcaagatt gaagctaatg cgtggaatac
      841 ttcagcaccc atacgtagag cttctttgaa tgttggagcg ccaacaggca tgatcatgaa
      901 ttcttggaag tcgattgagt tatcagcatg tgatccgccg ttgatgatgt tcatcattgg
      961 tgttggcaat acttttgtgt tgaatccgcc taggtagtgg tataaaggta cttctaggta
     1021 gtcagcagct gcacgtgcaa cagcgataga aacacctaaa atagcgttcg cacctaattt
     1081 acctttgtta ggagttccgt ctaaagcgat catagctttg tcgatagcca tttggtcacg
     1141 aacatcgtag ccaatgattg cttcagcaat gatgttgttt acgttgtcga cagctttagt
     1201 tacaccttta ccgccataac gagctttgtc tccgtcacgt aattcaactg cttcgtattc
     1261 accagttgaa gcacctgaag gaaccattcc gcggccaaaa gctccgcttt ctgtgtatac
     1321 ttctacttcg attgttgggt taccacgtga gtctaagatc tcgcgagcgt aaacatcagt
     1381 aataattgac atgttttgtc tctcctttga gtttgttttt tcctaaggga tttccctcat
     1441 atacgatttt agttaatttt gctcattagt gcaaacattt cttgcatttt tcgcaaattt
     1501 tcaacaatta ttttacagcg tctaacaatg acaagaatga atctgcttgc aagcttgcgc
     1561 caccgactaa agcaccgtca acattttctt ttgccatgta ttcagcaata ttttctggtt
     1621 ttacagaacc gccgtattgg atacgaactg cttctgatac ttcttttcca taaagttttt
     1681 caactgtttg acgtacaact ccacaaattt cgtcagcgat ttgtgcgtca gctgatttac
     1741 cagtaccgat tgcccagatt ggttcataag caataaccat gtttgacacc tgttcagctg
     1801 aaagatcagc taaaccagct gtaatttgtc cttcgatcca ttcagcagtt ttgcctgctt
     1861 cataggtttc taaagattcg ccgcagcata ggattggtgt cataccattt gcaaagatag
     1921 ctttcgcttt tttattgatg tcttcgtctg tttcatggaa atattcacga cgttctgaat
     1981 gtccgatgat cacataatga acacctagat cagcaagagc tgctggtgaa gtttcaccag
     2041 tgaacgcacc tgagttttcc cagtagcagt tttgtgctga gatttttagt tctgtacctt
     2101 cagctgatgt cattaattct tgtaagaata atgcaggaga tccgatgact gaatcaactt
     2161 tgtcatttga agggattttg ttttttactg cttcagcaaa ttcttttgct tctgatgcag
     2221 ttttgttcat tttccagtta cctgcgataa taggtttacg cattgaggaa cacactcctt
     2281 aatttattga aaaatggcta gggagtcatg aaaactccct tcaccttttg ttcgattact
     2341 cgtcagaatt gagcaaactt ggtcgtttat ttatcgttga ttgctgctag tcctggtaat
     2401 tctttacctt caagcaattc caagcttgcg ccgccacctg ttgagatgtg tgtgaatttg
     2461 tcagcaaatc ctaattgttc tgctgctgcg gctgagtctc cgccaccgat gattgttgtt
     2521 gcgccttcta agttagcgat tgcttcacaa acaccgattg tacctttagc aaagttactc
     2581 atttcaaata cacccatagg tccgttccaa acaactgttt ttgcaccttg taatgtatca
     2641 gcaaataatt ttactgatgc tgggccgatg tctaaagcca tcaaaccatc aggaatgtct
     2701 ccttcgatta cttgtgtttc aacatcattt gaaaattctg gtgcacagac attgtcgatt
     2761 ggcaagacaa gtttgtcgcc tgctttttcg attaattctt ttgctagttc cactttatca
     2821 gcttctacta gagagttacc gatcttgatt ccttttgctt cgtagaatgt gtaagtcatt
     2881 ccgccaccga tcaagatttt atctgcttta ggaatcaagt tttcgattac gccgatttta
     2941 tcagaaactt ttgcgccacc aaggattgca atcattggtc gttttggttc ttctactgct
     3001 tcaccgatga acttgatttc tttttccatc aagaatccag caactgttgg gataccagtt
     3061 gaagcgatac ctacgttaga agcatgtgca cggtgagctg taccgaaagc atcgttaaca
     3121 aatacatcgc ctaatgaagc ccagtattta cctagttctg tatcattttt gctttctttt
     3181 ttaccgtcaa tatcttcaaa tcgtgtattt tcgaatacga caacgtcgcc gtcattcatg
     3241 ttgcggattg cttcttctaa ttgttctccg cgagtttcag gtacgaatgt tacttcttta
     3301 cctaataatt cgcctagacg ttttgcaact ggagctaaag attttccttc tttatcttct
     3361 tctgttttta cacgtccaag gtgagagaaa aggattgctt ttccgccttg ttctagaacg
     3421 tagttgattg ttggcaatgc tgctttgata cgagtgtcgt cagtgatcac gccgtctttc
     3481 aaaggaacgt tgaagtcaac acggacaaga acttttttgt cttttaaatc gatatctttc
     3541 actgttttct tagccatttt atttcctcct gaataaactt attatcaaaa aaagcggaag
     3601 aagcgcgatt gcttccccgc ttttaaatgt acatgtctat aatttacact tgtacgtttg
     3661 tctatcttat aagttagcga agtattctaa agtacgtact aattgtgcag tgtatgacat
     3721 ttcgttgtcg taccaagcaa cagttttaac taattgtttg tcgccaactg tcattacttt
     3781 agtttgtgta gcatcaaata atgaaccgta agtcatacca acgatatctg aagaaacgat
     3841 ttcgtctgtg ttgtatccgt aagattcgtt tgaagcttct ttcattgctg cattgatttc
     3901 gtcaacagtt acttcttttt caagaactgt tactaattct gttaatgaac ctgttggtac
     3961 aggaacacgt tgagcagctc cgtctaattt accgttcaat tcgggaatta ctaaaccgat
     4021 agctttagca gcacctgttg agttaggaac gatgtttgca gcagcagcgc gtgcacggcg
     4081 gaagtcacct ttaggatgag gtccgtctag agtcatttgg tcacctgtgt aagcgtggat
     4141 agttgtcatt aatccttcaa caacaccaaa tttgtcgttc aatgttttag ccataggagc
     4201 taaacagtta gtagtacatg aagcacctga gataactgtt tctttaccag ttaatgtttc
     4261 gtggtttgtg ttataaacga ttgttggaac atcgtttccg ccaggagcag agataacaac
     4321 gcgtttagca ccagctgtta aatgtttttc agctgctgat ttagaagtga agaaaccagt
     4381 acattctaga acgatatcta cgcctagttc gccccatggt aattcttctg ggttgcggtt
     4441 agctaatact ttaacttctt ttccgtttac gttgaatgaa ccttcatgaa cttcaactgt
     4501 tccgttgaaa cgtccttgag ttgtgtcgta ttttaacaag tgtgccaaca ttttagcatc
     4561 tgtcaagtcg ttgattgcta ctacttcgat tccatctaca tcttggatac gacggaatgc
     4621 tagacgtcca atacgtccaa aaccattaat acctacttta actgtcattt aagatttcct
     4681 ccttatgaaa atcaaaaaaa tatttatttt aaagggttac cccttttaaa atctcatttg
     4741 cagcagcttc atcagtgatg agccacgttt gttttggtgc atttttcata taagctcgaa
     4801 ttgctttagc ttttgatttt ccgcccgcga ttgccatgat gatcgggact ttttccaaat
     4861 cttgcaattc tatgccgatt ctagggacct tataaacgac ttcacctttt tcattgaaaa
     4921 agtagccaaa agattccgca acagcatttg cttgtttcag catgactaat tctttttcag
     4981 tcatcttacg acgtgctgcc atgtgtaatg cacgcccaat actatgaata acacaattcg
     5041 cttcactaat caagtttaag acttcttgga tagatggctc attgagtaaa gaattgtaag
     5101 ttgccagact taattgttcc ggaacataaa gtgcgcggtg gttaccgttt gctttagcag
     5161 ccattcttgc actgacagaa tttgcctgta cagttatcgc ttcaccaatc ccgcctcttg
     5221 cagggacaaa caggttatgc cgtttcttat tttccagatt agacaattgt tcggctacca
     5281 cagccatagt agttcctccc atcacagcga tgatattctc gccttctgga agtagtcggt
     5341 ctaaggcttc gctcagcgct tcgccgaatg catcagccac tttggtctgc tcttcgctgt
     5401 ttcctgaaac aacgatacag cgttggttac caaaatattc tgccaactgt tgttcggttt
     5461 gatgcgtacc taaaagctga tccatgaagt tttcaaggct ttgataaact tcttcgccct
     5521 tagttgtcag cgtcataccg ctttttgagg tgtcgatcaa attcagattt ttcaaaaggt
     5581 ctgtttctgt tcgtaagact cgttctgtga tccccatact ttccgataaa cttcgacgcc
     5641 caatcggctg catccaatag atatttcgta aaattttata gcgttcttgc aacacttcga
     5701 taatatctgg tgcaacagat tcaatcattt ttatttcatc cagcatagcg atctccttag
     5761 ttggacactt tacgtccaat gtagacttaa aacgacccat atacgataaa aaaagaaatt
     5821 aactattcga atttcacatc aataaaggga aatctcaacc tcacgactgt aagtctaaca
     5881 gttttgctct cgtttttcaa ctaaaaagac tagaaaaagt gaaatacttt ttctagtctt
     5941 ttgactgatt agttatcata acgttttctt tttgatgaac tcgggattcc taataaatca
     6001 cgatattttg ctactgttcg tcttgagatc gctatctcat cttttttcag caattcaacc
     6061 aatttctggt cagacaacgg cttgtttttg tcttcttgat cgactaattc ctgcagcttt
     6121 ttcttcgctg tatctgctga aaggtcttct gtctgttctg tactattcgt aggaattcgt
     6181 gtagtgaaaa atttttttaa ttcaaataca ccgaagtctg tttccaaata tttcccgttg
     6241 actgcacgac tgaccgtgga ttcatgcaca tctatctcag ttgcaacttc ctttaacgta
     6301 agaggcttga ttggacgttc cttgtcgata aagaaagctt tttgccggga aacaatgact
     6361 tgagcaaccc gcaaaattgt atccccacgt tgtaggatcg ttttcttcaa ccattcgaat
     6421 tcctgtttcc gttcttttaa ataattttca gtttctttat ctgcttgttg tgacatttgt
     6481 ttgaagtaac tctcttgaaa gcgtatattt ggctgatttt ttcggtttga gataacttgt
     6541 acatttttat cgtcgtccac taatacccgg acatcaggaa taatgtacaa ttcgctgcta
     6601 cggtcgaaaa tccggccagg gcttggactc aacgtttgta aatagtcgaa tatctgctga
     6661 acagcatgta gatcaacaga aaaacgttgt gcgatttctt tccattttcg ctccaccagt
     6721 tcctcaaaac attcttccaa cacgatataa gctaattccg gcgcataatc atctcgttct
     6781 gtttgaagca tgagacattc ttgcaaacta cgtgcaccta ctccagctgg ttctaattgc
     6841 tgaatcagag tcaacgcatc gagcatttgg ataggcgtag ctccaatctc ttttccggct
     6901 tcttctaaac taatcgtcaa aaatccgttc aaatcgatgt attctacaag ataaagaacg
     6961 attttcctca aaaaagtatc tcggtaattc aaatggactt gttcgatcaa tgaatcaaat
     7021 aaagaccctt ttgtatcagg aatctgattt aggtaatttg tctcttctcc ttcataagaa
     7081 gatgaagaag cttttatgta atctggctgc cattccggct caacaacttc caccaatgga
     7141 ttttccattg cttgattctc aacgaatgcc aacagctcct cactgttata ttgtaaaact
     7201 tggatggact gttgcaactg ttgcgtcatg gctagtttct gtgtctgctg ctgtttttgt
     7261 gaaaaatttt gctcaaattt catttttttg tccaatcccc ttgtaatttt ttatctgttt
     7321 agttacaatt atcccagtgc ttgcgtcctt agtgtagtgg atatcacgta agattccggt
     7381 tcttgagacg ggggttcgat tccctcagga cgcgttctac tgctatttta gcataattta
     7441 cgaatagttg cttctttatt tttaagtgaa aactgtccgc caaa
//
LOCUS       DAZUKC010000110         7455 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000110 DAZUKC010000000
VERSION     DAZUKC010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.112"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(83..808)
                     /locus_tag="ACG80T_002464"
     CDS             complement(83..808)
                     /locus_tag="ACG80T_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842079.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(962..1288)
                     /locus_tag="ACG80T_002465"
     CDS             complement(962..1288)
                     /locus_tag="ACG80T_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842080.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(1325..1690)
                     /locus_tag="ACG80T_002466"
     CDS             complement(1325..1690)
                     /locus_tag="ACG80T_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFS4842081.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(1727..1942)
                     /locus_tag="ACG80T_002467"
     CDS             complement(1727..1942)
                     /locus_tag="ACG80T_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4842082.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            2071..2406
                     /locus_tag="ACG80T_002468"
     CDS             2071..2406
                     /locus_tag="ACG80T_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4842083.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(2482..4479)
                     /locus_tag="ACG80T_002469"
     CDS             complement(2482..4479)
                     /locus_tag="ACG80T_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842084.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(4577..4852)
                     /locus_tag="ACG80T_002470"
     CDS             complement(4577..4852)
                     /locus_tag="ACG80T_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4842085.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            5084..5818
                     /locus_tag="ACG80T_002471"
     CDS             5084..5818
                     /locus_tag="ACG80T_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4842086.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            5857..6999
                     /locus_tag="ACG80T_002472"
     CDS             5857..6999
                     /locus_tag="ACG80T_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4842087.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT         2506 a         1353 c         1080 g         2516 t
ORIGIN      
        1 ttatcctaag ctactggaga tgtataagta cggtcatata acaacggggt agggtaaata
       61 aatgaaaaag atggaagaat cattattcaa taactttttt aaatacttcg ttgtaatcag
      121 ttacctgtcc gttcttaaat accacagaat aatctttttt cccattttca agtaaccgaa
      181 gtggaatatc tttaccaact ttctctgtca ctgcattact gatacctgtt agattagcta
      241 ataaatctga taaagattga gcatctgtag aaatctctgt atcagtattt atcgtgattc
      301 ctttatcttc atttacaagt ttccaagtag gtggtatagc tgtgtatgtt tctacaaatg
      361 aattaagttt ttcattaagt gacttctctt ttatatttcc atctaaaaac tctgtatata
      421 tggcttgagc tatactaaaa caaagctggc cttgttctaa taattttaca tcattagtta
      481 gtaaagctgt gttcacgtta tcaatcgctt caagaagagt atctttggtg tccctaacag
      541 ctttaggtgt gccttctgga atttccctac ttgatacctc gtccatttta tcttccataa
      601 accccttaaa atcatatctt tctcccaaat ccatagaagt ttttgagaaa gctttcattt
      661 ctgagtctat ttcttcaata atatttaaca tgtctgatac atattgttta ggctgattcg
      721 tttttaatct atatgtggta aatatctcaa ttcctccaat tatcaataaa attgccaaac
      781 aaatccataa taattttttc tttttcattt ttcctcctat acactgctgt tataacagtg
      841 actaatgtaa agattttccc agcaatcatc agactattat ttacagggaa tcaaaaaaac
      901 tccttagtaa gcttacacct ttaggagttc ctgtttgaac ggtatgcata atacatttaa
      961 atcaatttaa cattaacacc tcagtatatg gtatctctac atatacacgg tctttttttg
     1021 gatagtactc gacatttata ttgatagcac ttccattgtc taacgtagct ttagcccggt
     1081 acgtgttatc accattatca tgagtgatcg tcacatcatc acatgtccga tttaagttat
     1141 actgttcttg taaaatctga gttaccaatg gtacagatga cttctctagt ccagatgaac
     1201 ccgtcattct tgaaaaaatt atacctataa tagccaatac aacgatacct ataatgacaa
     1261 acttcttatt catatttact ttattcaact ttttatctcc tatctccaaa atgattttct
     1321 ctcatcagta actcattgac atccccaata gtacaccaaa gatggctcca gctacccctg
     1381 ctatcatcaa aatcgtaccc atttgttcat ctttctctcg atataagtaa cccataataa
     1441 cagcaacagc accaaatacc acaggaataa atgctaatga tactaccata gaaatccaac
     1501 caataatcaa atacagcttc cctgtctctt gtccatgtac gatttgttcc ttcatttcat
     1561 taattgtctg ctcagtactt ttctttactg tgtctgttcc ggttgtctca tctgtttctt
     1621 gtaatgctcc gcaaaactgg caatacttac tttcatttgt tatttcttta ccacattttc
     1681 tacaatacat gtcttctcct cctaaaatca ttcaattgct tattagctaa ctgtttactt
     1741 tattaaatac atcagaaaac agctcattta ttggtacact taatatctca gaaagcattt
     1801 tcatttgata gggtttagga atcgttctcc ctgtttgcca cgaacttaca cattgctgtg
     1861 taacattcat ttttttggct aactcagttt gggaaagtcc tttttctttt ctgtatcggc
     1921 tgagattact tacttttttc atgattctcc ccttctgttt tattatatac aacaatatga
     1981 tgtatttcaa ctcataaaat atataaatac ttcaaatgtc attacaaata caacaacttg
     2041 ttgtactcta ctgagaaggg gggtgtatta atgaaacaga agcaatatgc aaaggtcatt
     2101 agagaaaagc gaaaagcact cgggtggaca caaaaagaat tagctaaaaa aatcttctct
     2161 acacaacagg cagtagcaag atgggaaaat tctgtaacag aacctaacct cgatagctta
     2221 acagctcttt ctagagccct aggaacccct gtaagccatt ttctagacaa tgttgacata
     2281 gattatgagg aagagttttt ggctctctat cgctctctta gtactgaaga tgcaattcga
     2341 acaattgact atatgaagct attaaaaagg caagaaaatg aacgcaatca gctattgaaa
     2401 gattgaaaaa taaataaccc accgaacttt aacggtgggt gtattgagat gaaataagat
     2461 taagagctat ggataacctg tctagtttaa tataattggg tctcttctat tccttactct
     2521 ccgtccatca tctcttgtta cacttgtaaa ttgataaata ggaactaatt caactcctga
     2581 taatatcaat tcttttatta cttcatctat ccctgcccct ttcgctagat atactaaaaa
     2641 actactgctt gctttcacat tgtatagttt cttaatatcc ttagaagctc gttcaagagt
     2701 gaatccttgt ttaacaaagt agcctctgtt ttctattaaa gcatttttca cgctgttatc
     2761 cctgacattt cgtaggcgtt cacaaacagt gttaataagt tctttttctg tcattagata
     2821 tgtcccttgc cacgataagc cctgtaaaac ttcatgtgct tcatctaaaa tcaattgttt
     2881 ttccttgcta agttctgatc gggaattttg aggaaatact cttttagcct ctttttcgtt
     2941 aaaacatatc ttaacccatt cataagaaaa atattgatat gtaaaatgaa atttttccag
     3001 aagtggaagg atatcttcat tcaatttttg aatcacagaa gttgcattaa aaacgtctaa
     3061 tcgatagaca ttgcttctct tatgtttaaa gtttcctaac gctttctctt cctttaacct
     3121 atttaggtat tcgagcatct tcaaaggaac atcttcatca tctaattttt ttatcaatcc
     3181 tataaaagtc ataagtgata gtaacttatt acatttatct atgtcataag ttttaccata
     3241 gtaagaactc atttttttag ctaattgttc ccccgacaaa atacaagtca cactttcctt
     3301 actatcatca aaaaaattaa catcagaaag caagacatca tagacacgtc gcatttcctc
     3361 tagataattc cttattgtcc tgaaaagttt gtctttcatt gattcaagag tgtcaaaaag
     3421 tacttttgat tcctctttca attttcgaaa atgtgtagga tacaagtcta ggttctctaa
     3481 tagccaatta aatgtttcta cccgactgtc tttctttaaa attagcatca caactccaat
     3541 aaaattttca ctgatatcac aattactgga atgacaataa aaaatggaat gcccagtttt
     3601 actggtaaaa atactagcag agggattatg atcgttgtga aacagacatc ttaagttttt
     3661 acttgactta agtaatttat tcatatcaat ggttaacaat ctttcataaa tctcattaaa
     3721 agacatgtcg gaggaatcaa acaggcagtc tttaaaccac tgtttcattt cttctttgtc
     3781 gtcatttttt atcaacttaa caaaatcggt ttcattgact agttcatact ctctccctga
     3841 atcactttga caatttttag aacatgtcat ggtaaaatca atcggaagct cagtaatatc
     3901 taacatgttg tcttcattaa ttattatcac tgcatctgac ttatatcctc caaaaaacat
     3961 tctattcgaa gcatttacat tgtcatcaca cccggggatg ttctttttca agtattcgta
     4021 aacagcaacc attttaccat agtcagtaat tgcttctttt aatagaaaga caatcttgta
     4081 tttttctaga ttttcagagt accgaatact tttttgtatc gcaaacgcag aattatgaac
     4141 taaattgata gataaaacct tttctatgct taagtatcta ctatcatctt tactcaaatc
     4201 tactaccttc cccttctcat ctttaacagt gttatcgaca tccagaataa ttactctggt
     4261 ttccacaaaa ctctccttgt cattgccttt attctttgtc ttgcttggaa taaatgattg
     4321 tgatgaattt agtatctcta caaactcatc tcttgtaact tctctatagt tatctaaaca
     4381 cctatctctt acatcagttt tttctttttt tgtaactggc ttctctaata atttttcttt
     4441 atctacataa attggaatat taatcattga tgtttccatt tgtgtttcct ccttataaaa
     4501 ttctgttaaa gactaaaaca tacagataat aaaaacaaac tctttattac tatatgtttt
     4561 agtcttattt gtttactcaa cctttatacc gttccatgaa actatcaata tcagattttt
     4621 taaatctaat gaccgtatca atttttataa caggtaaccc gtattgagaa acatattttc
     4681 taatcgttcc tactgataca cctagatatt cacttgcctg ctgatagtcc atatactcta
     4741 gagtgactcc agaaataatg tcttctagca taggctttag cactgtaatt atctctttca
     4801 cttgttgttc aacttgtata gaaacaatgc tatcaatgat ttgtagagac atatttcccc
     4861 ctcctttcgt gattgcttga acaaattttc taagaattat cttcgtattt tttgctcaaa
     4921 ataaaagtat tttgttatta accgattaat caaacaaaaa atgattacca gttcatcgac
     4981 atacacataa tacctcgata aaaaaggggt gtcaatagtt ttgtaaaatt tatttacaac
     5041 accccttttt tgaggtatac taattttagg aggtgtggtc aatatgataa aacgtccacc
     5101 aataaattat ttagaacgaa aaaagatact cggaacaaaa ataaaagcta taagaaagag
     5161 taaaaaatta acacaaccag catttggtct tatgattaac aacggtcaat taattgacaa
     5221 aaagactatc tacgaatggg agaagggaac ctatttacca ataccagagc gattatcccg
     5281 aattgctgat ttgggaaata tgagtataga ggagttagtt tgtggcaatg tagaagaata
     5341 tattttggga attattcttt atagagatag cattgtacta gatggtataa catttccaga
     5401 taaaaacctg ttccagcatc ttcgtcaaca gttcccacca gtacactcta atttagatac
     5461 atggctggat agatattcca aattagaacc agagatgcaa gaatttattg ctaacaagac
     5521 ttgtaataaa gttaaaaatg aaaaaattag tcttttcaat atcttaaaaa tagaagaatt
     5581 attcatcaat gctattgtag aagaatttga caataacatt cttttcctca ctagcagtat
     5641 agaagaacta ctagaaagaa tggtagatga atggctacct attcaattga aagacatgtc
     5701 ttatcccgaa gaagctgtga gagaaattac tgataatata aacaagctag aacaaacgat
     5761 ttcatcaatt gggaaaaaat acactaagaa aaaaatgaag ggaggtgata ccatctagtg
     5821 gtaagtactc cttgtaaact aagaggagat gatttcatgg caaaaatatc aaagtacaaa
     5881 aagaaaaacg gtcaagtggc gtggatgtat aaaggacaca ttgccaccga tccacgaact
     5941 ggtgaaaaaa taaatactac gagaagaggt tttagtagca aacaagaagc tcaaaaagat
     6001 tatgacgagt acagacatca aatattatac ggtgttaaaa agaaagcacc tgatatgaca
     6061 tttgaagacc tgtataatga atggataact catcagcgta ccagtgttaa agcttcaaca
     6121 atcgcaatct cagtaagata cgcaaataat caaatacttc cagcatttgg taagcttaaa
     6181 ctatcgaata tctctgtccc atattgtcaa aaagtggtag atgaatggca cagtaaatat
     6241 gaaagttacg attatatgag aaaacagact gcacaaattt tacgctacgg ggttgcaatg
     6301 caatatattg acaataatcc tatggaaaaa acacttcttc cacgaaaaaa agagtatgag
     6361 aaaaatcgta aattttactc aaaagaggag cttaataatt tactggatgc ttttaaagat
     6421 tttggtaaca tgaaacaata cgccttcttc agattattgg catacacagg tatgagaaag
     6481 agtgaagtac tcgctttaca gtggaaagac atagacacgt ttaataaaga actacatgtc
     6541 aataaaactc ttgctgtaga tgaatttggt aaagttataa ttcaatctcc gaaaacaaga
     6601 gcttctagac gagttatctc tttagatact gagactcttt ctatcctcaa caactggaaa
     6661 ttacagcaaa aagaagagta tttaaaactt ggatacaata ctagctctaa agagcaacat
     6721 gtttttacaa cagttaaaaa cactctatac attccaaaca ctgttaatga ttggttaaga
     6781 tatattttga aaaaatataa cttgcctcgt attacacctc atgggtttag acatacacat
     6841 gctagtttat tattagaagc aggagaatct gtcaaggtag ttcaacaacg attagggcat
     6901 gaaaatagta aagtgacatt agacatctat gcacatatta caaataatgc acctaagaaa
     6961 acaggtcaag actttgctga tatgatggct catcagtaaa agagctggtc aaaatctggt
     7021 catttctagt atacaggcaa aacaaaaaag ctagaaccct tatatatcaa gggttctagc
     7081 tcctataaca ggtttaccca acactgcctt ccaatgactc ttttgggaat cagaggaaag
     7141 aatcaggctt tatagagata tttcatctaa caaattactt tagactgaca gtatatatca
     7201 gcaggtactg tcatcgggaa cgtagccaat atcgtatcca aaacagcagg tagagaagct
     7261 atttgaggat tcaagttatt ctatttcaac catgaagtta agttcattaa aaataaagat
     7321 agttggtatt tcaataattt ttgtatagag ttaatcactt tattgatgct aattcatctt
     7381 gattggtatt ataatcgtat tcaagttgcg tggcgtgctt ttgctagaaa atatataact
     7441 gttctttata aagaa
//
LOCUS       DAZUKC010000111         7279 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000111 DAZUKC010000000
VERSION     DAZUKC010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.113"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(65..262)
                     /locus_tag="ACG80T_002473"
     CDS             complement(65..262)
                     /locus_tag="ACG80T_002473"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFS4842088.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(408..1286)
                     /locus_tag="ACG80T_002474"
     CDS             complement(408..1286)
                     /locus_tag="ACG80T_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS4842089.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1325..2443)
                     /locus_tag="ACG80T_002475"
     CDS             complement(1325..2443)
                     /locus_tag="ACG80T_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFS4842090.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2440..4539)
                     /locus_tag="ACG80T_002476"
     CDS             complement(2440..4539)
                     /locus_tag="ACG80T_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFS4842091.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4590..5411)
                     /locus_tag="ACG80T_002477"
     CDS             complement(4590..5411)
                     /locus_tag="ACG80T_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4842092.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5408..6217)
                     /locus_tag="ACG80T_002478"
     CDS             complement(5408..6217)
                     /locus_tag="ACG80T_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4842093.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6238..6732)
                     /locus_tag="ACG80T_002479"
     CDS             complement(6238..6732)
                     /locus_tag="ACG80T_002479"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4842094.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6744..7244)
                     /locus_tag="ACG80T_002480"
     CDS             complement(6744..7244)
                     /locus_tag="ACG80T_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4842095.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
BASE COUNT         2211 a         1683 c         1214 g         2171 t
ORIGIN      
        1 ctttttataa tcacaactct ctataataga attacgattt attttatacg gctgaaaaaa
       61 tctatcacca aaaaaattca aacattaagc caaggattat gaagtcaaca tttcgaaaaa
      121 tgaacataca ttggtaagca actataacta ttaccacaga cataaagtaa cacaatatat
      181 tactatttaa gtgtacgcca ccagtgaata tcctcaaggt atacagcgca caacaaaaag
      241 aaaaaaggaa acgcagaagc atttcctttc agtgatttaa tgaaaacaac acgatttgac
      301 ttagagcctt tttatttgaa ttcattttcg ctttatccaa cttcatttga tcatttgatt
      361 actcggttaa atacttttga catgagtatc aatgaaacat tcattaatta tggttgctta
      421 tttcttaccg cttttctggc caatgctaat ccgccgatta ttccggcatt atcacctaat
      481 tcgggcgtaa taatgtagct atcaattggt ggtatttcaa catatccatt tataagctca
      541 acaaatttat cctgaacttt tttcttcatg tgttcttgtt tcattacacc accgccaaaa
      601 ataattctat ctggcgaaaa catcagtgtt gtattgtatg cacattgggc aaggtaaaag
      661 gcttcaatct gccaaaacga atcgtcagcc aataaattct ggccttttac acccaaccgt
      721 ttttcaatcg ctggtcctgc tgccatcccc tctaaacaat cttggtggaa gggacagttt
      781 cctgaaaaat tgtcctttgg atgtcgcttg atcaacatat gccccatttc cggatgactg
      841 aacccttcca cgaaagtccc attgaacaag gcaccacctc cgatacctgt tcccacagtg
      901 aagtatgcaa cacctgctaa ccctttgcca gaaccagcaa catattcacc atagcaagcc
      961 gcattgacat ctgttgtcca ttcaatcgga atattgaaat attgctttag cgttcctaca
     1021 aaattgaaat tttgccaagc aagttttggt gttgatgtga tatgaccgaa actcgttgat
     1081 ttgcattgaa tatcaattgg accaaagcag ccgaccccga tgcctgccaa ttgttcctta
     1141 aaaggtttaa aaaaatcaat gacctgcttc attgtttcaa ctggagttgt tgtgggaaaa
     1201 ctgactcttt ccaaaatcgt taattcctca gtaccgactg cacagacaaa tttagtccct
     1261 ccagcttcaa ttgccccata tatcatcata ggttcctccc ttcgaatatg tgaaaaacac
     1321 ttcattaacg cggcaagcgt tctcgatcag tgaaatgacg tttagtattt cctgaagctt
     1381 ctgttgaata ccagtaagcg acactagcaa catcgtcttg ccgctcaaat aagcgaacat
     1441 catcgttgcc gatttgttgt aaagtgaccg ataaatcttc ggtaaaacag atggggtctt
     1501 gaagatgcca acgataaaga ccatgtcgtg gcattgcgtc gtcaccaaag gcatgaacgg
     1561 cattcaattt tcccgtttca aaacgatctc ttgtggcatc tctatttgat tggtaaggat
     1621 accctaaaaa ggctgttgaa tagtttttcg catgtggacg tccgaactca tcgtactcat
     1681 gaaaggccca cgccccgcca aaataatctt ctgaacctgt tgaggttacg gtcggatact
     1741 cactatcccc atcaatgtag aatttaaact cgccttcgcc ccaccaataa cgttctaggc
     1801 aggtaagcgc aaggtaagta cccacgtaat agccttgacc ctttaatcga tcaattaact
     1861 tataatcctc ctgtaatttg gtaatgcgtt cccgatgcca atttgcgtga aaatataggc
     1921 tattttcttc caattcgtcc accaaagcgt aatttatcgt ataaaaaaat gaggacatat
     1981 ccttcggatg taaattctcg atcgtaattt tagcggatgt ttggaaaggc atttcgaaat
     2041 aggaattaaa gccccctgta ggattaacca ctatcggcaa cgagttcacc tcgcaacgct
     2101 ctccaaaccc attacaaaag aaatccccta aaggactttc aacagcaggg ctatccgacc
     2161 catcccaata aattcttaag attaaatcac gtaaaacaaa actgccaaat tctgttcgat
     2221 cacgaatcgt catccacata tgacgaatta ttcccgttcc agtaatatcc gcaatcacag
     2281 tttcttcacc agaagtcagc ggaattgcgc cacgaccttt gcggcttggt cctagctcgc
     2341 ttggcaacat cgcagcttta cctttctcgc ctgttggatt ttcgggagtg attgctcgac
     2401 tctggatttt tttaaattgg gtaatatttt ttagtatact catctttttc tcctttgaat
     2461 gtgaatcgtt tttccctttt ctacgtgaag aatttttttc gtatcactta ttttcaatgg
     2521 cttttctgac aaatttgaaa tactcaactc ggattgcgta cattcaatcg aaatgaaggc
     2581 ttgaccgaac ttcaaattct tccaagaaaa cgttaaatta tcaacaggat caatttgcaa
     2641 tactcctttt ttgaatttca atccgaaata ttgactgatc attaggctga caaaaagacc
     2701 cgatgcccag ccgcatttgc cgcagccaaa gtctcgcacg acttctcctt gttgagcctc
     2761 taagcgatac ggccaccacc accacgaacc gtctaaatct gccaaacaaa ttagttcttc
     2821 taatcgggtt acgaaagctt gagggttgtg cagctcgctc attaaaacag tgataaagcc
     2881 aggaaaagtg gcgccggatt ctaatcccca agtgatgcct ttgatttcct cgctataggt
     2941 tggattgaaa gaactagcag aaaaggccat ggtgttctga tataaatgat ctttattatc
     3001 taaaaattga taaaaaggca tcaacgtagt gtctgattct tctccatcat gcatcaataa
     3061 attaattttt ccatccacaa tcacatcact taaaaagatt aacccttgct caagaatcgg
     3121 tttttgatag tgcttcactg aataggttgt tttctcctca tcaccgattc cctccaaaaa
     3181 ttgctcacca aaaggaccat cgatagtcat ttgtttacaa attgattcct ttagtttaca
     3241 ggcaatgttt ttgtatcgct tgctaagact cagttgttct agggcttcaa aaatttctgc
     3301 aagtccaata caggccttcc aaagacaaag attgctgccg gtatgatact ttccaagtgc
     3361 aaaggcgtct gataaccaaa cagatggaaa aagcattggt tcccctgacc gacgttgagc
     3421 taatatgtcc tcaataattc ttgaagcatt cagtagaacc tgcggacacc gctcaaagaa
     3481 ttttgtatcc ccagtatgtt caaaataaag actagccagt aacgcactgg ataacgaatt
     3541 actcagagaa tgattgatac ctcctgtaaa ttttgaacca ggaaacttaa cgccatattg
     3601 atcaaaccat agaaccgttt tttgacataa ctccggttca aacatggtga atggcaatga
     3661 tgcgtaatac atatccttat tccaaattcg attagtagca ggatagctgc cccaattgct
     3721 gccgacgacc tgtccctgtc gattcatacc gaaactagaa aagctttgat aaagtgcacg
     3781 acgatagaga tggacaactc tatcgtttgg cattgacagc tgcccaaata gctgagcaaa
     3841 atagttcaat gtttccttca gccatttttc gatatcagat tcctgaaaag tcattacttc
     3901 ttcaaaagca tttgggtcca caaaagcaat ggcgaagctt gccacttcct gttgtgcgat
     3961 cgtgtcctta tttccttttt gagaaatcaa cacattttgc tgatccgaat acttctcttg
     4021 atacgaaggc acatctttca ctgaaacttg aaggctttcg tgtgtttcat tcacaatgta
     4081 aacctgttga acaagcatac ttaaacgctt attagccagt atgggagcaa aggatactac
     4141 aatgacctta aacgtcgaat aatgatgaat atatctaggc aaacagtctt gaatcacatc
     4201 gacttcaata cgatgatccg attctgacaa atggtagaca atcccgttta cttcaacctt
     4261 taaggataac gaattggttt gagagagctg tttattgacc caaacacctg gtttttcctc
     4321 agttagtaaa ttttctcgat aaaaggtcaa cgtttgaatc gtcccgcaaa catcaaggtt
     4381 tgctaatgtc ttttgattac ttacatcgaa ggtcaatccc tttacatcag gaatattccg
     4441 atagtagctg tattcgtttg aagtagtcac atttccgtcg tttaactcag cattaaaaaa
     4501 tgggtgtttg ggtgaataaa ttacttcatt aatagtcatc ttttccctcc ttgctaaaaa
     4561 aactgcagcc tagtagctgc agcttcctac tatgcgattc caagtaatga caataaaatt
     4621 cccaagacga ccattccaaa aataattttg atgacgccga cttttttatc tagtaatttg
     4681 aaacagctca atgttaccaa taacggtaca attcctttaa aaatactatc gaggatttct
     4741 tggacattca ttagttcttc gcccttcatt gaaaagctca aaacggtatt gaatttgacc
     4801 atactgatcg tcattgcacc taccatgatc agacccaaga tactagcagc ttttgtcaga
     4861 atttcaatta acccgttcgc atacagcttt tcaatatatt tagagcctaa tgaatagcct
     4921 aaaacgccgc catagtatcg catcaagtaa ttaggtatat tgaacataac taaaaagata
     4981 attggtgcca aaatatttcc ggcattggct aagttgatcg ctagtcccgc ggtaacgaca
     5041 cgccaaactc cccagaacaa tgaatcgcca attccagcca atggtcccat caaactcgtt
     5101 ttgatcgcgt tgatactttc cacgtcaaaa ccagcttttt cagaattttc cttctccatc
     5161 gatgccgcaa tccccatgac aaaggacgcg caaggaatgg tcgtcgaaaa atactgtgta
     5221 tgacgttcca gcgccgcagc tcgatcttct tcacttttgt agaaatgatt aatgaagggc
     5281 atcatagaat aggcgaaacc accgccgccc atcgtcaccg gattcaccga agcatacata
     5341 gtcatcgagc gccaaaacat cttccgaacc aattttttct catctttaga aattccatca
     5401 gctaatttca ttgaaagaaa tcctcctctt cttggtcaag cgttgcattt acagcagcat
     5461 cgctttgaac tgcatgaaca gatatatttt ttagttgtag atctctttgg gctataacaa
     5521 ttgcaataat cgttccaact gcggcaattg caatcaatgg taactcgaga tacgccataa
     5581 caacggcccc aaagaaaaac cacacagcaa tatttttact ccaaagcatc cgtaacagca
     5641 tcgccatccc aactgccggc agcagatttc cagcgactgt taatccgttc atgatgacct
     5701 ctggaatatt atccaagacg cttttgacaa catctgctcc aaataaaacg gcaaagaatg
     5761 gaataagagc ataaagcgca tagtggacaa accacataaa gaagtgcaga ttgatgattc
     5821 ctttttgatc accagatttt gcaaatttga aaaatttagc agccatcggt ccacatacaa
     5881 aagaaaaaat catattcatt aactgtaatc ctaatactgc tagtggaaag acaatgggaa
     5941 taatcgcatc cacgtttcct cccattttga ttgcgaacgc tgtagccagc ccagcagcca
     6001 aagaaggctc cgccgtacta gagattccga tattgacgac acccataaag atggcttcta
     6061 gtgacgcccc gacaatcaac cccgtctgca aatcccccat caacgctcct gctaacggag
     6121 caacaacaat cggacgactc agcatggaca tcccgaacat ttcatgtcca gcaactccaa
     6181 taaaaacggc caaagcgact acaaatgcat catacataat ttttctcctt cctttttcta
     6241 aaaatcactc aaaatagaga tagctgaacg tttttccatc gccggtgtag gctgaaaaac
     6301 ggtatctgga taaagttcaa ctaattcttt taagtcttga atttctgacg tcgttaacat
     6361 cacttccttt gataaggttt tcttatcttc cgatggcggt gtcattttcc cagcattccc
     6421 cacgttcatt aattcgattt ccgttgggaa agctttcgca acttttaccg catctttgac
     6481 attagggact aaaaccatca agcgcatgtc cttcgcccgc ggatctttta acaaatgaat
     6541 cgcgtcatca actgttttaa taatgacttt gacattcgat ggtgcagcca tcttcaatgt
     6601 catcttttga atatcgttgg tagccgcctc atcgcttgct actactaacc cggttaaatt
     6661 tagactcttt gtccaagtca tcgctacttg tccatgaatt aatcgttcat caattcgcac
     6721 tgctttaatc atttttcttc ctcctaaaaa aaatcttcct cttcatttac tacatgtacc
     6781 tcatcaagaa tggtcatctt taattcctgt tggcatttat ttaccagttc ctgaatatcc
     6841 tcctttgtta attccttggt tgccaatact atttcgagca gaattggcag attaaagcca
     6901 gacaaaatag ttacagcaat ctctttctcc aaaaaggttt tggtaatctt ctgattgaca
     6961 cttccgccaa ataaatcggt aaaaacaaaa atctgatcct gctcgttcac ctggtttaca
     7021 aatacattca actcctgttc aaaatcacac tcatccacat aagcattaat caccgtaatc
     7081 tcttgaacct tccccgtcag taattgaata ctgctaagaa agcctgaagc taagtgaccg
     7141 tgagtcgcta ttagaatttt cttcatatgg ttaattcctt tctttctgtt cacttttata
     7201 taagcaattc ctgtgccaac ttttaaaaac attggtttaa tcacgttaat tcacattaaa
     7261 aaaagtgtct aacctaaaa
//
LOCUS       DAZUKC010000112         7169 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000112 DAZUKC010000000
VERSION     DAZUKC010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.114"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(63..515)
                     /locus_tag="ACG80T_002481"
     CDS             complement(63..515)
                     /locus_tag="ACG80T_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFS4842096.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            665..1615
                     /locus_tag="ACG80T_002482"
     CDS             665..1615
                     /locus_tag="ACG80T_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS4842097.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            1608..2567
                     /locus_tag="ACG80T_002483"
     CDS             1608..2567
                     /locus_tag="ACG80T_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS4842098.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            2564..3319
                     /locus_tag="ACG80T_002484"
     CDS             2564..3319
                     /locus_tag="ACG80T_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4842099.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            3342..4295
                     /locus_tag="ACG80T_002485"
     CDS             3342..4295
                     /locus_tag="ACG80T_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4842100.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(4939..5235)
                     /locus_tag="ACG80T_002486"
     CDS             complement(4939..5235)
                     /locus_tag="ACG80T_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFS4842101.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            5590..5946
                     /locus_tag="ACG80T_002487"
     CDS             5590..5946
                     /locus_tag="ACG80T_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS4842102.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            5956..6855
                     /locus_tag="ACG80T_002488"
     CDS             5956..6855
                     /locus_tag="ACG80T_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS4842103.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT         2202 a         1214 c         1434 g         2319 t
ORIGIN      
        1 tatttgatgt caatctgatc aaaagctttt tttgcatgta aaaatttatg tgttttttcg
       61 ttttaaaata aacgatccca gaagcttttt ttcttttttg gtttttcttt ttcagctggc
      121 gaagagtttt cttctgggta ttttttttcg atatcaatca caggttcatt tactgcttct
      181 tttgctgtta agagcaatcc gaattcctca ggatttgcag tgacgtaatc gttgactaca
      241 gtgaaacggc tattgttttt cgttgctagc tggatatatt ggttttggac agaaaggggt
      301 agagccccat tcaagagtat ggaagcatct ggatgttttt tgagttcttt taaaaagttt
      361 tctttgtttt ttggttggat catttcagca acagtcatac taagatagca gcgttccctg
      421 aatgtcccta aatatttatg ctgttcttca gggttcacta aaggtgtacc ataaagtccc
      481 ttatccaaat ggctttttac atcatcttgt gccataaaaa ataccctcct tcttctcttt
      541 gtctttctta cgattatagc atattcccaa taatacctaa aacatatgaa aaagcggttg
      601 cttttgttca acgattcact taaaataaga atgaaaatca ttctcaatta gaaaggtgga
      661 ttggatgcgc tacggacgac tagtagcatt attagttatt ttaagtagta tttcattgtt
      721 catcggagta tcgaatgtac aattgtcaga actgctccag ttagatgggc aaagcttaca
      781 aatcttgcta gtgactcgtt tgccacgaac gatttgtttg atccttgtgg gagcgaccag
      841 tagtatttgt gggttgatca tgcaacattt gacccaaaat aaatttgttt caccaacgac
      901 tgcgggcacg atggatagtg cgcgtttagg tattttagtg gctatgattt tcctacctgg
      961 tgcatccttg attgtccgat ccttgacagc attttgcttt gcttttgcag gaacattgct
     1021 ttttttatcc cttacgcgat tgttcctcca gaaaaatcaa ttgatgcttc cgttgattgg
     1081 agtcatgtta gggaatatca ttggttcagt tgctactttt tttgcttatc aattccagtt
     1141 ggtacaaaat atgacttcat ggcttcaagg gaattttgcg actgtgatac gaggaaatta
     1201 tgagttattg tatgctgttg ttccattgtt gctcttggtt tggctatttg cttaccgatt
     1261 tacgatcatt ggtctgggcg aatcgtttgc agtaaatctg ggaatagatt accagaagat
     1321 gcagttttta ggcattggct tagtatcatt ggctagtgca gtcatgttga tcatggtagg
     1381 aagcattcct tttatcggag tgatcattcc gaatcttgtc tctagaatat atggagatca
     1441 agtgcacaaa acaattggcg taactgcttt agcaggtagt ttgttcttag tattttgcga
     1501 tcttgtcgct cgcgtgttga tttttcctta tgaaatacca gtcagtgtga tcgttggaat
     1561 catcggtggg gctatcttcc tttatcttct gataagggga cgacgatatg cttaaaaaaa
     1621 agccatctat ttttattggt atgcttgttc ttgttttatt tgttacagga atgtacttga
     1681 cgatccatac atatggaaac tggcattttg catgtgcatt gaggagtaag aaaatttttg
     1741 catttatcct tgtctcgcta gctgtcagca gtgccacaat cagctttcag acaattacgc
     1801 ataaccagtt tttgactcct tcagtattgg gacttgagtc gttatatgtc cttgtccaaa
     1861 cggtcctttt cttctttatc ggaggagtga cgatgcttca gcagacaggt acagtttttt
     1921 tcttattgaa cgttgggctg atggtcgggc tgagtctatt gttatttttc tttcttttga
     1981 aaaaaatgag agcgaatctt tttttattac tgatgtcagg gatgatttta ggtacgcttt
     2041 ttggtagtgt cagtaccttt ttacaagtca tcatggatcc gaatgaatat gatttgcttc
     2101 aaggcagatt atttgcaagc tttggcaata tccagagtca gtatcttttg cctgcttctt
     2161 tgatcattat cggctgctgg tacctgttat ttcgatatcg acatgtgcta gacgttttcc
     2221 atctcggaaa tgaacaagca gtaaacctcg ggatagacac agatcgtttt gaatttttga
     2281 ttttatgttg tgtcagtctt ttgacaggga catcaacggc tcttgtgggt ccagttactt
     2341 ttttagggtt tattgtcgca aatatcagtt atcgtgtctt ttctacttat cgtcatggtc
     2401 agctatttat cggaagcttt ctggtagcta tgctgtttct gacaggagga cagctcgcag
     2461 tagagcagat tttcggctgg aatacaacag tgagtgtcgt cgttgaattt gtcggaggaa
     2521 tttattttgt cacaaaattg attcgcgaaa ggaagagtat cctatgattg atttacaaaa
     2581 ggtcaataaa ctgtaccatg gaaaatcagt ggttaaagat gtacagttaa cgattcctga
     2641 aaagaagttg actgctttta tcggtccaaa tggtgctgga aagtcaacgg tgttgtcgat
     2701 gatcagtcgt ttgataccaa aagatacagg tgaaatctat ttagaccata acgaagtaaa
     2761 ggcttggaaa tccaatgaat tagccaaaaa attatcgatc ctacgacaat cgaatgaaat
     2821 aaatgtccaa atcaccgtga aagaattagt agaatttgga cgatttcctt atacgaaagg
     2881 aaaattgacg acaaaagacg aggaaatcgt tcaggaatca ctggaacaca ttgggttaga
     2941 agaaatagcg gatcaatcga ttgacactct ttcaggcggt caactccaac gtgcgtatat
     3001 tgcaatggtg ctagcgcaag acacggatta tattttactg gatgaacctt tgaacaatct
     3061 agatatgaat tttgcggttc aaatcatgca gattttaaaa aatttagtac aagaaatcgg
     3121 gaaaacaatt gttattgttc ttcatgatat caactttgct gcaagttatg cagacgaaat
     3181 tgttgcaatg aaagatggac agttatttac gcaaggaacg acccaagaaa tgataaaaaa
     3241 agatgtattg gatgaattat acgaaatgga catacggatt tgtgagattg aaggccgcag
     3301 attctgtctt tatttctaaa actaacatta ggagagaaga gatgaaaaaa ttagtagtag
     3361 gtatcttgat tctagcatca tttggtttag cagcatgtgg aaatagcaac aacaccagtc
     3421 aggcagacac aaaaaaaagc agcacacaaa ctgaaactac attgacgata acagatagta
     3481 atggagacca aatcgaggtg ccaaataatc cagaaaaagt cgtcgtattc gacaatggtt
     3541 ctttagatac gatggatgct cttggtgttg gagataaggt agttggagca gctacaagca
     3601 gccttcctga gtatctttca tcctataaaa aagtagaatc agcaggtgga ataaaagaac
     3661 cagacttaga aaaaattaat cagcttcagc cagatctaat catcatttct ggccgacaaa
     3721 gagattttca aaaagatcta agtcaaattg ctccgaccat tttcctttct ttagatgcga
     3781 aaaatccatg ggaatctttc cagcaaaatg tcactgcttt aggggagatc tttggtaagc
     3841 aagaagaagc taaaactcaa ttagaggaat tatctagtgc aatcgatcag acgaaaaaga
     3901 aagcagaagc tactgacaaa aaagcactag tcacacttgt gaatgaaggg caattatcag
     3961 cctacggatc tggttctaga ttcggtttca tccatgattt atttggcttt gaacaagcag
     4021 atgatcaaat cgaagcatcc actcacggtc aaagtgtttc ttatgaatat gtgctggaaa
     4081 aaaatccgga tatcttattt gtagtagaca gaacaaaagc tattggtgga gacgacagca
     4141 aagatgatat ttctgccaat gaactggtag cacaaacgaa tgctggtaaa aatcagcaga
     4201 tcatctccct tgaaccagat gtctggtatt taagcggggg cggtttagaa tcaatgaaac
     4261 tgatgatcga agatgtgaat caagcattta aatagcataa ataacagagg attaaactct
     4321 catactatgt aacaataatg tttcttacat tcttaaatag caagagccta tcctcaaaga
     4381 agttatcaaa aaccatccgt aaaatcgtat tgatgatttt acggatggtt tttatttatt
     4441 tgagaaatct tttttcaacg tgaattcatt atctacatca tacgggttga ttctagaatg
     4501 gggagtggca aaaaatagtg tgatccattt tttcagcatc aagatcgcct cctttgattt
     4561 gaatcaaagg tacaaagcga aacttagata aaacttaaat ttagatattt ttattttgta
     4621 ccgatctctt ctttaaacga tgttccatct gctgcttctc ggcaataagt ccactagact
     4681 agaaaatatg agaagctatt ttctgtccag cgttcttatt actcaaaaca aatcgcagtt
     4741 gtcacaatct cttctaaacg atgttccatc tgctgcttct cggcaataag cccactagac
     4801 tagaaaatat gagaagctat tttctgtcca gcgttcttat tactcaaagc aaatcgcagt
     4861 tgtcacaatc tcttctaaac aatgttcaat cagctgacct tcggtattag ctcaaagcac
     4921 tgatttcaca acctctattt aggcatgtgt ctcagcaagc ggttgatagt tgatgatctg
     4981 catttttcga taaaaatcgg cgggaagctt actccaccgt tttagtaatt gatagaaata
     5041 actctcttct gctttgattt tatactcgct taatatcaat ccaaaactat tcgttttttt
     5101 tgaagctaat tttttataaa tatccaatgc tttggtttta tcttcattga caaagagtgt
     5161 ttttacttca atataagaac cctcgatatt ttgtgtttct tcgataaaat agatgttttt
     5221 tatatattcc ttcatagtga ttcctccaaa gaagtctttt atttgataag tttactctac
     5281 cgaagagttg ttaataatcc gtgaacaaaa agtaattctt tttccaatag ttgttaaagg
     5341 ttttttattt tttatagtat tttttgttat ttaagaaagg aataaggtta aacaaataac
     5401 aggttgcttt ttgggtcttt ttccgttcaa gaaactgaac gaagaaaagg gaaagttgtc
     5461 tcttttgtga taagagaaat cataataaac gaatgactta ctttccaata aattgagaac
     5521 agtcagaacc agacaaatta gttgcatctg atgaataaag tgattattct atgacatgag
     5581 gtgatacaga tgaaattaga agtgacaaaa gaagcacaag aagtattgaa gaataaatta
     5641 gaagcagacg atcaactttt attagatatc gaaaacggtg acggtccatt tgcagaaagc
     5701 caagtcagtt gccaattgga tacggcattt cgattgatca ttgtcaaaaa agatacacaa
     5761 acagatctaa gtctttattc tgtagtggct gatacaccag ttggaccaat caaaatcaaa
     5821 gacagtgcaa tactttacat ggatgatcca tccacacttg ctttagaacc aacttataat
     5881 agtttacaat taaaaggacc aagcggcttg agaaaaggca acttgcaagt tgttcgaaaa
     5941 gactagaggg ataaaatggg aaaattggcg atcatcagcg acctacatgt ggacatcaat
     6001 aaattcggcg aatcagagct gatgcagctt tggcaagttt tgcaaaagca caagatcact
     6061 cggcttcatc tagccggaga tacggctaat aaagtagacc gttgcctagc tgttgtcgaa
     6121 tttttcgtca ataaaggatt atctacgaca tttcatttcg gcaatcacga gttggcagat
     6181 gtgcccaatg aacagatgat ggaccatttt ccagacccac attttttaaa tgaacggtat
     6241 attgctttaa atgagcagac cgttctttta gggatgaatg gctggtacga ctatcagttt
     6301 tccgaattga aagattacga ggcaatcgta cgaatgaagc gtttgtattg gtatgatcgg
     6361 ttcatccagc gtgggaaaac agatctcgag gtcaatgcag aaatgcttgc agctgcaaaa
     6421 aatttgctag accagctgga agcaaaaaaa cttgaggtga ttttatccac tcatttcgtt
     6481 cctaaacggg catttatcat gtatcaatct gctccttatg agcgttggaa caaattaaat
     6541 gcatttttag gatcagaatc attcggagaa ttacttgacc gttattcaaa tatcaaacaa
     6601 gtagtttttg gccatacgca tcgtcgcttt gaagataaat ccatcggtca cacgatttac
     6661 agctgcagac cttttggtta ttattatgaa tggcagctga cccgagattt tgtcttgaaa
     6721 gaacagttgc tagacagcta tgatcctatg aaattacgtg tcctcttgcg tgcccatcac
     6781 ccagcttttc aggcctatca aacacaacat ttgcaagagg aatttgaaca agcgatgaca
     6841 atcgttccgt attgagacag cattagtaat gtgtaaaaga aaataccttg taaaacataa
     6901 ggataagtaa gagcagtaga tccatttatc gtggatatgc tgcttttttt gcggaaaata
     6961 atcagcgaat gtttatttga tcggatgatt tgtaactcgc agagtactag atgcaagcgt
     7021 gtattctttt tttgaacgat aatcacaaaa aaaaatataa tatacatttt ttatttaatt
     7081 ataattaata ttgattgaag tctaggcagt caagtcgaga gtgtaaaata ttttgtgtaa
     7141 atgaaaaaat ccatacaaaa aaggaagtc
//
LOCUS       DAZUKC010000113         7127 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000113 DAZUKC010000000
VERSION     DAZUKC010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.115"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..525
                     /locus_tag="ACG80T_002489"
     CDS             <1..525
                     /locus_tag="ACG80T_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFS4842104.1"
                     /translation="SEKAIPYIAAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTI
                     SNEINQYREREPEIQPAEVKQEHKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRT
                     LHLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKN
                     EIYYEQQLSQKERV"
     gene            586..2046
                     /locus_tag="ACG80T_002490"
     CDS             586..2046
                     /locus_tag="ACG80T_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFS4842105.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            2057..4261
                     /gene="topB"
                     /locus_tag="ACG80T_002491"
     CDS             2057..4261
                     /gene="topB"
                     /locus_tag="ACG80T_002491"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFS4842106.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAESIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ENDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            4277..4426
                     /locus_tag="ACG80T_002492"
     CDS             4277..4426
                     /locus_tag="ACG80T_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842107.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            4500..5093
                     /locus_tag="ACG80T_002493"
     CDS             4500..5093
                     /locus_tag="ACG80T_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFS4842108.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            5109..6008
                     /locus_tag="ACG80T_002494"
     CDS             5109..6008
                     /locus_tag="ACG80T_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFS4842109.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            6011..6496
                     /gene="ssb"
                     /locus_tag="ACG80T_002495"
     CDS             6011..6496
                     /gene="ssb"
                     /locus_tag="ACG80T_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS4842110.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            6862..7122
                     /locus_tag="ACG80T_002496"
     CDS             6862..7122
                     /locus_tag="ACG80T_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842111.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
BASE COUNT         2625 a         1215 c         1483 g         1804 t
ORIGIN      
        1 tcggagaaag ctattcctta tattgcagca tggacgaaga atggtcagac gttggatgat
       61 aaggaagcgg cggaacgtgg gaaaatcatg aatgatgtca gccgtgtcgc aaatgaattc
      121 attcaaacca tttctaatga aatcaatcaa tacagagaaa gagaaccaga gatccaacca
      181 gcagaagtga agcaggagca taaaaatact gtagaaacgg cagagaataa gaatattgtg
      241 cccgttggtt cactttggat cgacaagaaa gacgggaaag taatggaaga agatacgggt
      301 cgaacgttac atttgaagga tgcggcgttt attgaagatc cgcagcataa tcaagtgatt
      361 ttaaaaggaa aaagttatgg aaaagagatt caaacggcgt taccttcaga agaatttgcg
      421 ctaggaaatc cattccgatt ttcacttgaa acagtgtcag aagtggccaa acgaaaaaat
      481 gaaatctatt atgaacaaca attgtcacaa aaagaacgcg tataaaacaa gtaaaaaaga
      541 ctatctgaaa aacggatagt cttttttctt ttgggaggtt tgaaaatgac cgaaaaaaaa
      601 atgagtctta ttgatcgatg taaacagatc gatatagtgg attttgctcg aaacaatgga
      661 atggctgtag tcaataaagg acgagactat cgcttagaag accatgattc atttgttttt
      721 gatcgaagaa aacaacgttt ttattggaac agtcaaaata ttagtggcga cattattgaa
      781 ttagctaaac tttttttcat agacaaagag atccaagact ctaagcaaca atttaaagca
      841 gccactgatt ttattctaaa gaatgaagac aaaacagaac gagtggaaaa tcttcatttt
      901 gaaacagaaa agtacaaaga tcaccccgtt gattatcaac cactcacaga aaaaggaagg
      961 aattatctga aggaagaacg aaaactacca gagtggctga ttgattatgc agaaaaagaa
     1021 gggttgattg ccgaactaaa gccaaaatat gaacggcaaa atttcttagt tcgagacgat
     1081 cgtttagatc atgcggtcgc ctttttatgg aaagatccac agacaaaaga gacagtaggg
     1141 gcgagttatc aaggaacgtt cattgattat gaacgttttg gtgagagagg cacatataag
     1201 catatagata aaaattcgac agcgaatcat ggctttaacc taaagatcgg tgaccctaag
     1261 cagttaaaat tttttgagag tagtattgat ttacttagct atgctgcact gaatcgtgat
     1321 cagttaaacg atacgtggtt agtctctatg gaagggttaa aacaccatgt gatcagtcat
     1381 tattttggtg aggcagtatc tgaattgaga aagaaacaag cctttcctca atccatcgag
     1441 atttgtgtgg ataatgaccg tgcaggacat attttctacg aaaaagaaca attaatgggc
     1501 gcagtcgatc catttacgaa tcagaaagta cgttgtgaac gaggaatcgc taatgattgg
     1561 caagtgccaa aagaatacaa aatcatttat gaagaagtcg caaaagaaga aaaggtcaca
     1621 ccagaagcta ttatggccat acacaagaca gaaaacaatc tgcaattaac gaaccaactg
     1681 gtttccgctc acaaagtaaa tgcgtttttt ggccagcagc tttctgttaa cgattcaata
     1741 gaagccatta atttgaaaga tatttgtaga gaagtcgcaa aagaattaaa agtttgtgaa
     1801 cgcgtggatg gtacgtacga ttttgatcgt ttttatcaag aaaaagggga tattaatgcg
     1861 cagattcttt tctcttataa agcagaacaa tactacaaag gctataaaaa tcatgaacat
     1921 gaatttatac ctgaagtaaa aaaggactgg aacgatcaat tgaagcatga gatccagcaa
     1981 caagaaatca gaaagcaaaa acgtgccatg ttgtttcaac aaggcagaca acaagaaagg
     2041 gagtgaaatc gtcatgatga aaaccgttat tttagcagaa aagccgtcac aagcgaaagc
     2101 ttatgccgat agcttctcta aagccactcg aaaagacggg tattttgaaa tccaagatcg
     2161 attgtttcct ggtgaaactg tgatcacgta tggttttggt catttagtcg aattagattc
     2221 gcccgatatg tacgatgaaa attggaaaca atggtcatta gaacatttgc ctattttccc
     2281 tacacattat cactatcatg ttcctaaaga taagaaaaag caattcaagg tggtgaaaca
     2341 gcaacttcaa tcggccgaca ccattattat tgcgacagat agcgatcgtg aaggggaact
     2401 gatcgcttgg acgatcattc aacaagtagg cgccgatcaa ggaaaaacct ttaaaagact
     2461 ttggatcaac tccttagaaa aagaagccat ttatcaggga ttccagcagt tacgagacgc
     2521 aaaagagacc tatccaaaat ttgaagaagc acaagcacgt cagatcgccg attggttaat
     2581 tgggatgaac ggtagcccac tgtatagttt actcttgcag caaaaaggca taccaggaag
     2641 cttttcctta ggcagagtcc aaaccccaac gctttacatg atttatcaat tgcaggaaaa
     2701 gattagaaat ttcaagaaag aaccttattt tgaaggaaaa tccgaagtaa tcgctcaaaa
     2761 tggcgcattt gatgcgaagc ttgatccgaa tgaaacccaa gcgacgcaag aagcatttga
     2821 agattattta aaagaaaagg gggttcaatt gggaaaacag ccaggcatga ttcgccaagt
     2881 tgaaaccgag aaaaaaagcg cggcaagtcc acgtcttttt tctctatcca gccttcagtc
     2941 taaaatgaat cagctcatga aagctagtgc caaagacact ttggaagcca tgcaaggctt
     3001 gtatgaaggg aaatatctaa gctatccacg aacagacacc ccttatatta cggaagggga
     3061 gtatgcgtat ttgttggatc atttagatga atacaagcat tttttaaagg ccgaatcaat
     3121 cccaacccct attcatacgc cgaatagtcg gtatgtgaat aacaagaaag tccaggaaca
     3181 ttacgcgatc attcctacaa aaacggttat gacagcagcg gcttttgaac aactaagtcc
     3241 actgcagcag gccatttatg aacaagtgtt aaaaacaacg gtggccatgt ttgcggaaaa
     3301 atatacctat gaagaaacaa cgatcttgac gcaagtccag cagcttcagt taaaggcaat
     3361 cggaaaagta ccgatagatc taggttggaa gaagttattt ggcaaagaga acgacggaaa
     3421 agagaaggaa gaagagccgc tacttcctaa agtgacaaaa ggagaaatgg tgacggtaga
     3481 tctacaagtc cttgaaaaag aaaccaagcc accacagcct tataccgaag gtacgttgat
     3541 cacggcgatg aaaacagctg ggaaaaccgt tgatagtgaa gaagcgcaat ccatattgaa
     3601 agaagtggaa ggaatcggta cagaagcgac acgggcgaat attattgaaa ccttgaaaca
     3661 aaaggaatat atcaaggtag agaaaaataa gctcgtggtg acgaataaag ggattttgct
     3721 ttgccaagcc gtggaaaaag agccgctgct tacgagtgcc gaaatgaccg ccaaatggga
     3781 aagctacttg ttaaagatcg gtgaacgaaa aggaacacaa accacttttc tagccaatat
     3841 ccaaaaattt gtctctcact tattggaagt cgtaccaggg caaatccaat ccactgattt
     3901 tggatcgacg ttacaagaag tgaaagcagc cagtgaaaag caagaagccg cgcgtcattt
     3961 aggaatttgt ccaaaatgcc aggaacaaga agtcttactc tatcaaaagg tggcagcatg
     4021 tacgtctgaa gcctgtgatt tcaaactatg gacaacgatt gccaaaaaga aacttacagc
     4081 gacacagctg aaagagatca ttcaaaatag ccgaacaagc caaccagtga aagggttaaa
     4141 aggtcagaag ggttcgtttg aagcaacgat tgttttaaaa gaagacttca ctacaggctt
     4201 tgaattttct gaaaagaaaa aaacgaactt caagaaaaga acacgacgca ctacaaaata
     4261 atggagggtt aatcttatgc gtaatacaac acacaaacaa caagaaatcg aaaatcaaaa
     4321 agccaaaatt cgttcagaac aggaaaaaat gcgtgccagc aaacgaagac tgcagcaatt
     4381 gaagaaccag cagcggattc aaaaagaaat cgaattagat cgataaattc ctttcttgtt
     4441 aatataaaac taacatgtta tagttgtgtt agttttatat taactttagg ggaagagtaa
     4501 tgaaagacaa aatccaatgt atttttgata aacataatgg caacctatcc ttgaaggatg
     4561 ctagaagtga aggattatcg cctatgacgt tagatcgatt agccgaaaag gataaaatag
     4621 aaaaggtagc accaggtttc tataccttaa aggatgcgtt cgtggatgaa ttgtatctcg
     4681 ctcagagtat ctattccaga gggatttttt ctcatgagac tgcgctcgat ctttttcaag
     4741 taggaacata tatccctaaa aagatcaata tgatgtttcc taaaggatac aatgtatcaa
     4801 aggagaaatt agatcagtat gccattcaac cgcattacac taataaagaa aattttgacc
     4861 tgggagtcac agaaacagaa agcttttacg gcaataaaat tttagtctat gatttagagc
     4921 gaacgttatg tgacatgtgg aatccaagat acaaagcaag cgtagaggta aaacaagaag
     4981 cattaaaaga atatatgaca agtaccagta gaaatacaaa taagctaaga agatacatgg
     5041 atattttaaa atcaccaaaa gaaatgactt tgtatatgtt gccactttat tgagaggagt
     5101 aaagatgaat gacaccgaca caatttaaag agaaaacaag aaatttcgca gcagagaaac
     5161 atttaaaccc acaaattgtc caaagatatt atatgatgga aaaattatta gaatgtattg
     5221 ttgaatcgcc ttatcatgat gattttgtat taaaaggggg atttctgatt ggatcaaaat
     5281 acgggttaga aaaccgttca accgttgata ttgatacgac ttatcgaaat tcgaaactaa
     5341 caaaagaaaa gttagaaact gttttttctg aattaacagc taatcctaca aaagatggaa
     5401 ttcgttttga attaaaggga ttaacagaga cgagggaagc cgattattat ccaggttttc
     5461 aagcaaagtt tattgcttat cttgaaaatg cacgtgtacc ttttaaatta gatattactt
     5521 caggtgattc tattattcca gcagcattga tgtatgaaca caaattaatg tttgaagata
     5581 aaacgattca tattccagct tatcctacgg aacaaattct agctgaaaaa ttacatgcca
     5641 cgtttagttt tggaaaagat aattcgcgca tgaaagattt ttatgacctg tatatgattc
     5701 cgaaattaga acgtatcgat gagaaaaatt tgtataagag tgttcaagga acattcaagg
     5761 aaagaaataa tcaatcggtg tttaagcatt tgtatgatca ggaaatgcct caattggtga
     5821 aagatgaggg cttgagagaa aagtggaaaa gataccaaca agaaaactat tttgccaaag
     5881 acatttcttt cgaatctaca ctaacttccg ttgattccct gatgcaatca attgtagagc
     5941 aagagaaggc agaacgaaaa caaaatttta agagatcaca acaaatgaat caagaaatgg
     6001 agagataaag atgatcaata atgtaacttt agtagggcga ttaactaaag atccagattt
     6061 acgttatacc gcaagtggca cagcagtggc gactttttcc ctagccgtta atcgtaattt
     6121 cacgaatcaa aacggcaatc gagaagctga ttttatcaat tgcgtgattt ggcgcaaacc
     6181 agcagaaacg atggcgacat tagctaaaaa aggcagcttg atcggcgttg tcggacgaat
     6241 tcaaacacgg acttatgaca accagcaagg acaacgtgtg tatgtgacag aagttgtggc
     6301 cgataacttc caactattag aaagtaaagc agcgacagaa agccgtatgc acgctgatca
     6361 atcgtccagc agtccaagcc ctaccacctt tgaacaacgg gatacagcga cgcctaacaa
     6421 caatggattg aacgcttcac agaatccatt tggtggacaa tcaatcgata ttagcgatga
     6481 tgatctaccg ttctaagtag cattttctat tggttgcatt tgctacggtt tatgttataa
     6541 tttagacaac aaaaagaatt ttcaatctat aaccaaaaac cactagctgg cgggctagtg
     6601 gctttctttt acgtttctat tataagaatt aacgggtagg cttactgtct ttcttggcaa
     6661 atttacaatc aagccatttg cagatatagt gcgcggctac ttctgccatg attgcaaaaa
     6721 caaaatcaaa aagaattttc aaacctataa cctcctcccc ttgccagatt aggccggggt
     6781 agcagacagc aagactatta tagcataatt gaaatatcca aatgattgga tttttgctat
     6841 aattttagcg aggtgattgt gatgaacaaa tatgttggtt tgcttgataa aatccgagta
     6901 ataaaaacgc aaccgttatt ggttcgcttt actttacaaa cgattcatga atcgattaat
     6961 tgtgtagttg cagatattga aattatcgat aaattgctaa ttatggatga tggaaaatac
     7021 aatattgccg tcacaggtca cttcaataaa cgaaatcagc tagttattgc tagcatgtat
     7081 gttagaaatc ccgatcattt tacgcgatcg atgggcatat aaataaa
//
LOCUS       DAZUKC010000114         7092 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000114 DAZUKC010000000
VERSION     DAZUKC010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.116"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(718..3867)
                     /locus_tag="ACG80T_002497"
     CDS             complement(718..3867)
                     /locus_tag="ACG80T_002497"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFS4842112.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYAQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3881..5125)
                     /locus_tag="ACG80T_002498"
     CDS             complement(3881..5125)
                     /locus_tag="ACG80T_002498"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFS4842113.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELIKFKLEPGDFIMSGAGTIGRIS
                     RVPKQIKPGVFNQALIRLRINKEITDSEYFIQFIRADFMQRKLTGANPGSAITNLVPM
                     SEVKKWIVQFPILEEQKKIGNFFKQLDDTIALQQRKLDLLKETKKGFLQKMFPKNGAK
                     VPEIRFPGFTEDWEQRKFKEFSKKTGKKNTKDLDFPAYSVSNKAGLISQTEQFDGSRL
                     DDLEKTNYKFVEPNEFAYNPARVNVGSIAFNNLGMTVIVSSLYVVVKMSEDLDNEFIL
                     QFIKSPTFIKEVKRNTEGSVREYLFYENFANIKFPFTRNKEEQQKIGAFFKQLDDTIA
                     LHQRKLDLLKETKKGFLQKMFV"
     gene            complement(5118..6710)
                     /locus_tag="ACG80T_002499"
     CDS             complement(5118..6710)
                     /locus_tag="ACG80T_002499"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFS4842114.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHE"
BASE COUNT         2002 a         1446 c         1129 g         2515 t
ORIGIN      
        1 ggactaatac aggtagtcct cttttttgtt attttgatat tctaaatcat tttttattgg
       61 attattccct tgttcgatat agacccaaat tgatctatca ataattatca aactgagtca
      121 tatagagaca aaaatttcaa aaatgacctg ttaaatggtc atcgaatatc atttaaaatc
      181 aatacgacgg agtcagacac ggagtcaaaa caagcccgct gagagtaaaa aatatataca
      241 gtcccatagg taaagtgcca caggactgta tattttttta tttgagatta cctgaaatga
      301 ttccatttac tttttatctc attttttcct actagttagt atcaaatact ctcctatttt
      361 ctcaataaac tattttgggt tacatggtat ctgaaacagc taaaaccttg tcattacagc
      421 aaaaaatcac tttatctccg tttcatttca gatacatttc acttaagcgg attccaaaaa
      481 aacaaaagaa aaaggaaccc ctagttagcc tatgcttttt attggttcct ctcaaaacac
      541 ggtcatataa caacggggtg gggtaaatga atgaaaaagg tggaggaaac attattcaat
      601 agctgtctta aatacttcgt tataaaatgt gaacaaataa aagacatcta tccctatttt
      661 ctacacttcc actttactac catctttgaa tcgtacaatt ttcttaactc ttctcgttta
      721 cctcaattca ttttcaagtg gaatcagctc atcatctaac attttacgcc atgctcgctt
      781 catttcttgt gcgtatttaa atggcttcgc ttctggatgg ttggctttat atccttcgta
      841 atccttactg ttgataattg ctttcatatt aggaattgga tcttcacctg gcatatattg
      901 cagattagaa gcatgtaatt ctttctcaga aacaaaccac tcattggaaa agacatgaat
      961 caaatcatca ctcgcttgtg taaaatagcg acgtttaatg acaaagatat cttcattact
     1021 atctaaagtc cccacatcaa acgcatttaa aatttgtcgg taaacttctt tgactccttc
     1081 tgttttattc aatttagcca aagctttttc aatttcttct cgtgcatctg aactattcgc
     1141 cgcataagtt cctagtaatt gatcaatata agtagaatca atatcataca acttcgtggt
     1201 attttcacta taaaattcga tcgtggaaaa atcttctccc tctttttctg gttcttcctc
     1261 tattaatgaa cccttcacgg tctcgtacac gccaatttgt tcttccaaaa gttgaatttg
     1321 attgttcaag gttgaagatt tcaccatgtc atcattgtaa tcgtcataag tcactaacga
     1381 ttcataagca cgattcaatt cttggaatgc tttgacgtac tcgatacgga tttcaagtgg
     1441 cgtatgttca tcaatggatt ccatggatga aacctctttt aacttaccat atgcttgctt
     1501 gaatcttttt ttggattcct gataacttgg ataaatcaaa cccgattctt cttttgcttc
     1561 tgaatacagt tttgtcgcat cctcgacatt cttctccatt gtggctggct ttcggaaagt
     1621 aacaattagt ccttttgttt tttctggata cgtacggttc gtacgtgaaa aagcttggat
     1681 caatcctgca tactctagat tacgatccac aaatagggtt tgaatcgtag gtgcgtcaaa
     1741 acctgtcaat agacgatcca ctacgatcac taaatcaatt tgtttcccaa attgtttgaa
     1801 ttccgctctt tttcgtgcca aacgattatt gatgtctccg ttataacgct caatgtctgc
     1861 taatgaccag gaagtatcat aatactgatt atattcctca ataatttttt gcatttcttc
     1921 ttgttggaca cttgaatctc ggctgttttc ttctaatgaa taggtaatag cgattcgtgg
     1981 aaaatcagga tcctccatcg ttcttccttc acgaatgggc tgattaggaa actcttttgt
     2041 taaccagtca ggttctttcg tcatttcttt gatcgctcga taatactttt tggccatgtc
     2101 aatggaactc gtggttaaga tcgcagactt cgttggacgg ccatttcgaa aatcaaattt
     2161 tgtataggca ttgtctggac ggaacatttt atgaatgact ttttggatat gttcatctgc
     2221 ttcaaaaaca gtagggtcca aataactttc ttttttgaca ggctccattt gatcaatcat
     2281 acgattgatc tgttccggtg aatactctgc atatgtttct acttctttta atttccgata
     2341 gattttattc tctaatgaag tcggctccac ggtgttttca tgctctactt gaaatcctaa
     2401 aaccgagcca tcttctaatg cattcttgat ggtataggta tgcaagacct ctccatactg
     2461 atcatgggtc gtacgtgcca attgcccttt ggcttgtttc cgattctcag ggaaaatcgg
     2521 tgtacctgta aagccaaacc aagtggattt cggaaacatt tttttgattt cttccatatt
     2581 ttcagcactt aacgctcgat gacactcatc tactacaaac acgatatgtt gccccattaa
     2641 tttttggaag cgattggttc cttttttctc ttcttgtttt tttgcatatt tcaacgccgc
     2701 atctagcttt tgacgggttg taataatcac aacatttgca ttggcatctg ccaacagagt
     2761 ttcacttaac tctttggcac ttcctgttcc cacgatcaag ctattcgctt tggcgttccc
     2821 ggaagaaatc ccagtattaa attccgaagc aaatttcgta aactctgtgg tggtttgatt
     2881 atctaaatct ttgcggtcca ctaacatgat cgtccgatcg atccccgatt ttcgtgctaa
     2941 aagcttagta gatacaaaac tagtcaacgt ttttcctgaa ccagtcgcat gccacacata
     3001 tcctgattga tgtttatttg cagcaataaa caaggcttga atcgcatgta cttgataagg
     3061 atgtaacacc atgaggattt tattgtcttg atcttcactg acaatcgtat aatccgcaat
     3121 caagcgatga gcatccggga tatttagcac ttgtttgcaa aactcatata agttttctac
     3181 ctttttatta tctttcgttc gccaactaaa taaaaactta ggatgcaaat cctttggtaa
     3241 ggcatttgca aaataccgag tggtttgttc attggaaacc acaaacaact gtaaggtcga
     3301 aaagatattg ttccgaaaca taccttcttc tgcatatttt ttgatttggt taaacgcctg
     3361 ataaaaacta tcttttgcgg ttacttgttt cagctcgatt tggacaatcg gtaatccatt
     3421 gatcagtaag gtgacgtcaa aacgtcgatc ccgtgcttct atgttacttc cacgttttgc
     3481 aatttgatgg accacttcat aggtagagat tcctccacca atgtcttgat tagaataaag
     3541 aatgagcgag acagaaccca gttgcggatc ttcccgttca atcgtgatcc gagccattcc
     3601 attttcacct tttaaccatt tcgcagcatc aaagggggtt ttcgttcgta gtaacaattc
     3661 tgttttgatc gtttcaaatt ctttatcggt caaaggaaaa tcattcagtt ctgcttgatt
     3721 atttgagatg attttttggc gtaaattgac ccaaagatct tcttctgatt ttagatcggg
     3781 acgatacgtc cactgattat gcccttcccc caatacacgg atcaactgtt cttcgacttc
     3841 cgcttcctca cggtgactaa ttttcattta tcttccctcc ctaaacaaac atcttttgta
     3901 agaagccttt tttcgtttcc ttcagcaaat ctaacttacg ttgatgaaga gcgatcgtgt
     3961 cgtcgagttg tttgaagaaa gcgccgattt tttgttgttc ctctttattc ctagtaaaag
     4021 gaaacttgat attagcgaaa ttctcataga ataagtattc tcgtacgctt ccttcagtgt
     4081 tgcgcttgac ctctttgata aatgttggtg acttgataaa ctgaagtatg aactcattat
     4141 ctaagtcctc gctcattttc acaactacat aaagagagct aacaatgacc gtcataccaa
     4201 gattgttaaa tgcaattgat cctacgttta ctcttgctgg attataggcg aattcatttg
     4261 gttcaacgaa tttataattt gttttttcta aatcatccaa cctactacca tcaaactgtt
     4321 ctgtttgaga aattaaacct gctttattac ttaccgaata ggcagggaag tctaaatctt
     4381 tcgtgttctt ttttccagtc ttcttactaa actctttgaa cttacgctgt tcccaatctt
     4441 ccgtaaatcc aggaaaccga atttctggca cttttgctcc gtttttaggg aacatttttt
     4501 gtaagaatcc ttttttcgtt tcctttaata aatctagctt acgttgttga agagcgatag
     4561 tgtcgtccag ttgtttgaag aagttcccaa ttttcttttg ttcttctaat attggaaatt
     4621 gtactatcca ttttttcact tctgacattg gtacgagatt tgtaattgct gaacctggat
     4681 tagctcctgt gagttttctt tgcataaaat ctgcacgaat aaattgaata aaatattctg
     4741 aatctgtgat ttctttattt attctaagtc taatcaaagc ctgattgaat acacccggtt
     4801 ttatttgctt tggtactctt gaaattctcc caattgttcc agcgccactc atgataaaat
     4861 cgcctggttc taatttaaat ttgattaatt cattatattt tttttcagta attttatatc
     4921 ttgtttcgta attatcataa atagcatttt gttgttcata gacaacaaaa ttgctattag
     4981 atacaaagaa ttctttcttt aaggcactcc caaatggtcc cctccttact ccatcttttt
     5041 tatcaagtaa gcattcaaac ttacgctgtt cccaagcttc cgtaaatcct gggaaacgaa
     5101 tttctggttg tatatcatta ctcatgtttg aacacctcca gcgcaccttg taaccacgct
     5161 tcattttcag gtgttgtttg cagggaagag atagcttcta aaagttcttt ttctagcact
     5221 tgctgttctt gtcgtatctt cttgatgtca tttccgacat gaaccatatc aattggttct
     5281 tcttcttcaa acgtatctac atagcgtgga atatttaagt tatagtcatt ttctttgatt
     5341 tcatcaaagt ttgccaaatg agcgtatttc tcgacatctt ttctttcagc ataggtttcg
     5401 aggatttttt gaatgttttc ttccgataat ttattctgat tttttccttt gacaaattct
     5461 cgactagcgt caatgaataa tacgtctcga gtttggcggt tctttttcaa tacaatcacc
     5521 gtcgttggaa tcgatgtccc gaagaacaag ttcgcaggca taccgatcac agcataaata
     5581 ctgccgtctt ctagtaattt ttgacgaatg accccttctg cagcgccacg gaataaaact
     5641 ccgtgtggca agacgatggc catagtccct gtttccttca aatgatagaa accatgaaga
     5701 agaaacgcaa aatctgcttt ggattttggc gctaattttc cataacgatt aaaccgagaa
     5761 tcgtctaaga aggtcgtatc tgctgaccaa ttagccgaat aaggtgggtt cattactact
     5821 gcatcaaatg tgtaaggttc atctgttggc caatccttat tcaacgtatc cccattgcgg
     5881 agattcattt cttcggcatc taccccatgt aaaatcaagt tcatttttgc tagattgtat
     5941 gtcgttgtat ttaattcttg cccatgatat ttaacgttat ctgggtgagt taagtagtta
     6001 cgcacattta acatcaatga acctgaaccc atggttggat caaaaacact aaagaatcgt
     6061 cgttcttttt gatccaacgt cacgatttgt gccatcatat cagaaaccat atgaggcgtg
     6121 taaaattctc cggctttctt ccctgcttct gaagcaaatt gactgatcag aaattcataa
     6181 gcatccccga tcacgtcccc atcgtgacct aaaacttcca cgtcattcaa tttcttaatg
     6241 acttcggtaa tcgtcacatt tcgttgttgc tcatctgttc ctagtttttt tgattggaga
     6301 tccacatcat caaataaccc attaaattga ttgtaggtag atgccaattg cacaaaggct
     6361 ttgtttagat cattcaattg gaacgttgct tgttttgctt gatccgctaa gacgttgaaa
     6421 agatactctg gcgaaatcgc ataccccaaa atatcaacga ttgttgcaat cagatcttct
     6481 tttgattctt catctgaaag taattcgcga tacaacatcg tttgtttgct taccgtatca
     6541 tattcctcta aggattcatc tgctaaaaga accacttgtt ccaacaaacg gtcggataaa
     6601 tatttataga aaatcagtcc caataaataa tttttatatt ctgacgcatc cattttgcta
     6661 cggagattat ctgccgcact aaataatttt tgatttaatt ctgcacccaa aaagatacac
     6721 tcctatctct tatttaaagt tgctatatac cctcttattt tagcatactc ccttttagaa
     6781 aaagcaaaaa aaaaaagaaa gaaacttatt cttgctaata aactacgcga aaggagtcta
     6841 acgttgtttg atataactta gactgatcgt taacttattt tttatatttc tacatagtta
     6901 tctttagaac catttcagat acatatgaaa caattacata tgattttttc ataaaaaaac
     6961 agtactgtct agtagtataa aatatacaaa tttcttaaaa attttttcac taaaatcact
     7021 acctgaaaca gtctaaaaca cttatatatc aacgtttaat gaaactatct ccgtttcatt
     7081 tcagatacaa at
//
LOCUS       DAZUKC010000115         6954 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000115 DAZUKC010000000
VERSION     DAZUKC010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.117"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..443)
                     /locus_tag="ACG80T_002500"
                     /pseudo
     CDS             complement(1..443)
                     /locus_tag="ACG80T_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(954..1496)
                     /locus_tag="ACG80T_002501"
     CDS             complement(954..1496)
                     /locus_tag="ACG80T_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBECR4 domain-containing protein"
                     /protein_id="HFS4842115.1"
                     /translation="MSIKETTQITRQLNAIYKAAHLLQEHFVDKKVSFVGEVSTVAII
                     FSTTNFMHLCGIDYRRGTHLFFQDALDRKINLQDIQIKTDGTTFQKLQVIGSLDLLLG
                     KHISIVGRGVYSSLRYDAAIRTRKKILALSLKQNGLIYIPISLLNLSSKEIGPGQKVT
                     GIFSEDLTSGELKMIMEVID"
     gene            complement(1809..2360)
                     /locus_tag="ACG80T_002502"
     CDS             complement(1809..2360)
                     /locus_tag="ACG80T_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4842116.1"
                     /translation="MGYNVQPLRSQQEINDFLFCLRRNKNAERDVFLFLIGINSGLRM
                     SDIVKLKKKDVITSKNPRIVEQKTGKTRILYLSSLQDLIHGYTAPLDPEAYLFPSTKG
                     GHLEVNTVYQMFQKVAKLLGRDDIGTHTLRKTFGYHYYKKTKDVATLMEIFGHSSEKI
                     TKRYIGINEDEISETLLNFRLGF"
     gene            2677..3075
                     /gene="merR"
                     /locus_tag="ACG80T_002503"
     CDS             2677..3075
                     /gene="merR"
                     /locus_tag="ACG80T_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0045340 - mercury ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0046689 - response to mercury ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hg(II)-responsive transcriptional regulator"
                     /protein_id="HFS4842117.1"
                     /translation="MAYFISSFSEKCGVNKESIRYYERKNLLPEPDRTNSGYRVYSEE
                     DVKRVKFIKHLQEIGFSLNEIYKLLGVVDKDNIRCEGMFEFVSKKEREIQKKIENLNR
                     IEYMLKDLRARCPDEKELYACPIIEVLIEN"
     gene            3089..4729
                     /gene="merA"
                     /locus_tag="ACG80T_002504"
     CDS             3089..4729
                     /gene="merA"
                     /locus_tag="ACG80T_002504"
                     /EC_number="1.16.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016152 - mercury (II) reductase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0045340 - mercury ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046689 - response to mercury ion [Evidence
                     IEA];
                     GO_process: GO:0050787 - detoxification of mercury ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury(II) reductase"
                     /protein_id="HFS4842118.1"
                     /translation="MNQFRMRIQGMTCTGCEKHIEKALESMGALNAKADFSQSEAVFQ
                     LPDDYAIENAIEAVKKINYQPEEVEKLPVQKRVMTNNKEDYDLLIIGSGGAAFSAAIK
                     AVEYGSKVGMIERGTVGGTCVNIGCIPSKTLLRAGEINQSAHVNIFEGLHTSTGEVEL
                     DRLVDQKNELVDDLRKQKYTNLIDDYGFDLIKGEAKFIDENTVTVSGQTYSAQRFLIA
                     TGASPLLPRITGLKEVDYLTSTTLLELRKVPKRLTIIGSGYIGRELGQLFHNLGSEVT
                     LIQRSKRLLKEYDPEISEAMEKALIEQGIKLISGATYERVEQDGNIKKVHIIVNGKNK
                     VIESDELLVGTGRKPNTEVLNLSAAGVQVGPNNEIKINAFAQTSNRKIYAAGDVTLGP
                     QFVYVAAYEGGIAAGNAIGGLNKAIDLSVVPGVSFTSPQFATVGLTQQQAKEKGYEVK
                     KAVLSLENVPRAIVNHNTTGVFKLVVDRKTQKILGVHIVSENAGEVIYGASLAVKFGL
                     TVADLKDTLAPYLTMSEGLKLAALAFDKDISKLSCCAG"
     gene            complement(5254..5739)
                     /gene="vanZ-A"
                     /locus_tag="ACG80T_002505"
     CDS             complement(5254..5739)
                     /gene="vanZ-A"
                     /locus_tag="ACG80T_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFS4842119.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(5892..6803)
                     /locus_tag="ACG80T_002506"
     CDS             complement(5892..6803)
                     /locus_tag="ACG80T_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFS4842120.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         2228 a         1239 c         1238 g         2249 t
ORIGIN      
        1 ggcctttcgg ctgcggataa attcttcagc gactgcttct tcaagttctg attcgtcttt
       61 ctttggtttt gattgataat aataggactg acgtgataga cctaatattc tgcacatcgc
      121 tgatataggg taaagatgct tattcgcatc gattacttgt ctcttcgtcc gaatatcagc
      181 gctgcttgct ttaaaatatc attttccatt tctaattgct ggtttctttt acgtagttct
      241 aacaattctt tttgttcagg cgtaggatta tctttttctt tgaatgaacc actcgtttta
      301 gattgcttta cccatttgtc aaatgctgaa gccgttagtt catattctcg aatgatttct
      361 acacgtggct ttccagctaa gtaaagattg acgatttgtt gcttgaattc ttgtgaaaaa
      421 gttcttcgtg ttctcttaga cataaaaatt cctcctggta tgttttcttc tagtctacac
      481 accttaattt ttctgtctag ttaattgtag cctatccaac ttaagattaa tgacactaaa
      541 ttaacagact atacgtagta tctatttgct atgaccatat cttatatcca taagtcaact
      601 cagccggact aaaagggcta atcttactag aacacaagct gcacttgata tgcaaattaa
      661 ccctgaatcg gaaaggtgct taaattggat attaacggag ggataaagta aaatttatct
      721 ctagggaatg tttagaaagg atccaagttc cttaacaagt gacaattagt aatgtagtaa
      781 caacgataga ttgtatgccg aacatttcaa tttgatacgt ataaaagacg atttcaagct
      841 catccgtgat aaataaccca catgaatgat ttgaaatcgt cttaaaatgc tttattttat
      901 aacccaaaat agaataatta cttcaaaatg acgtcgcata tcactattaa tgattaatcg
      961 atgacttcca taatcatttt aagttccccg gatgttaaat cctcgctaaa aatgccagta
     1021 actttttgtc caggtccaat ttcctttgat gataggttta acaaagagat agggatatag
     1081 attaatccgt tttgtttcaa agacagagcc aatatttttt tccttgttcg aatagctgca
     1141 tcatagcgaa gagaagagta aacacctctg ccaacaatag aaatatgctt ccccaataat
     1201 aaatcaaggc tacctattac ttgtaatttc tgaaaggtcg tcccatccgt tttgatttga
     1261 atatcctgta aatttatttt cctgtctaag gcatcttgaa aaaacaagtg tgtgcctctg
     1321 cgataatcaa tgccacagag gtgcataaaa ttagttgtcg aaaaaataat cgctaccgtt
     1381 gagacttctc cgacaaagga tactttttta tctacaaaat gttcttgtaa gagatgagca
     1441 gccttataaa tcgcgtttaa ctgtctggta atttgggttg tctcttttat agacatgcgt
     1501 ttgtattctt tttttgccgc gtgcgaagaa tgctcctttt tcccaataaa aaaagctgct
     1561 caaaagagca gccttttgta caggtttagt tttggtgctt gccacccgcc ggacccttac
     1621 gttccggatt tgtatcgagt ttagttttgg tgctcgccac ccgccggacc cttatgttcc
     1681 ggatttatat caagttgact atgagcctgc tctcatttgt atatataata taccaaaaaa
     1741 aggttgattt gtccaatact gtgggtgatt ataaccgtct tactgtgctt atctattata
     1801 taatacaatc aaaaacctaa ccgaaaattt aataacgttt cactgatttc atcttcatta
     1861 attccgatgt agcgtttggt aatcttctcg ctgctgtgac caaagatttc cattaaggtg
     1921 gccacgtctt tggttttctt gtaatagtgg taaccaaagg tttttcgtag ggtgtgggtg
     1981 ccgatatcgt cccttcctaa cagcttcgcg accttttgaa acatctggta gaccgtattg
     2041 acttctaaat ggccgccctt agtactggga aacaagtaag cctccggatc taagggtgcg
     2101 gtatagccgt gaatcaagtc ctgtaagctg cttaagtata aaatgcgtgt ttttcctgtc
     2161 ttttgctcca cgatgcgcgg gttttttgag gtaatcacgt cttttttctt gagtttaacg
     2221 atgtcggaca tgcgcagacc gctgttaatt ccaattagaa acaagaaaac gtcacgttcc
     2281 gcatttttat tccgtcgcag gcaaaataaa aaatcattaa tctcctgttg cgaccgtagt
     2341 ggctgaacat tatagcccat gacaatccct cctttttagt ctgttcgaat acatgattat
     2401 agttattaca acacgaaatc gtgtgtttag ggggagtaaa aaaaaagaac cctataagga
     2461 taggattctt gatacatgat tttatgtaat ttcatgtatt taggtccgat attatttatt
     2521 ataaaagaag tctcaaaatt tattggtacc acatttaatt ttcacttcat ttttgaaagt
     2581 aaataagaag gaaaatgata ttcccccctt cgtattattt ttggttggac atgtacctaa
     2641 gtacatggtt tataattaaa aaaaggatgt gatagcatgg cttattttat tagctccttc
     2701 tctgaaaaat gtggtgtaaa taaggaatcc attcgatatt acgaacgaaa aaacttacta
     2761 ccagaacccg atcggactaa ttccggttat cgagtttatt cagaggaaga tgtaaaacga
     2821 gtaaaattca ttaaacacct acaagaaatt ggcttttctt taaatgaaat ttataaatta
     2881 cttggtgttg tagataaaga caacattcgt tgtgaaggta tgtttgaatt tgtctctaag
     2941 aaagaaagag aaattcaaaa aaaaatcgag aatttgaata gaatagaata tatgctaaaa
     3001 gatttacgag caagatgtcc agatgaaaaa gaactatacg cgtgtcctat catagaagta
     3061 ttaatagaaa attaacgggg gtaggaaaat gaatcaattt agaatgcgca ttcaaggaat
     3121 gacctgtact ggctgtgaaa aacatataga aaaagcgctt gagagcatgg gagctctcaa
     3181 cgctaaagca gatttcagtc aaagcgaagc ggtatttcaa ttaccagacg actatgcaat
     3241 agaaaacgcg atagaagcag ttaaaaaaat aaattatcag cccgaagaag tagaaaagtt
     3301 acctgttcaa aaacgcgtga tgactaataa caaggaagat tatgatttgc tcattatcgg
     3361 ttctggtgga gcagcttttt cagcagctat aaaagcagtc gagtatggat caaaggtggg
     3421 catgattgaa cgcggaactg taggcggaac ctgtgtgaac attggttgta tcccttcaaa
     3481 aacgctgctt cgagctggag aaatcaatca atcagcacat gtaaatattt ttgaaggatt
     3541 gcacacttct acgggagaag tagaattaga ccgcttagtc gatcaaaaaa atgaactagt
     3601 tgatgattta cgcaaacaaa aatacactaa tttaattgac gactatggtt ttgatttaat
     3661 taaaggagaa gcaaaattca ttgacgaaaa cactgtgaca gttagtgggc aaacatattc
     3721 tgcccaacgg tttttaatcg caacgggtgc ttctccactc ttacctcgta ttactggact
     3781 taaagaagta gattatttaa ctagcacaac cctccttgaa ctaagaaaag taccaaaacg
     3841 attaacaatt attggatcgg gttatattgg aagagaactg ggacagctct tccacaatct
     3901 gggttctgag gttaccctta ttcaaagaag caaacgactt ttaaaagagt atgatccaga
     3961 gatttctgaa gcaatggaga aagctttaat cgaacaagga ataaagctca tttccggagc
     4021 aacttatgaa cgagtagagc aagacggaaa tattaaaaaa gttcatatta ttgtaaacgg
     4081 caaaaataag gtcattgagt cagacgagct actagttgga acaggaagaa aaccgaatac
     4141 agaagtattg aacttgagcg cagcaggcgt tcaagttggg ccaaacaatg aaatcaagat
     4201 aaatgctttt gctcagacaa gtaatagaaa aatttatgca gcaggagacg tgacattagg
     4261 acctcagttt gtctatgtag cagcctatga aggaggaatt gcagcaggaa atgcaattgg
     4321 tgggttaaat aaagcaatcg atttatcggt agttcctgga gtatcattta ccagtccgca
     4381 atttgcaact gttgggttaa cacaacaaca agcgaaagaa aaaggatacg aagtaaaaaa
     4441 agcggtattg tcattagaaa atgttcctag agcgattgtc aatcataata cgacaggggt
     4501 gtttaaactt gtggttgata gaaaaacaca gaagattttg ggagttcata tcgtttctga
     4561 aaatgcagga gaagttattt atggagcatc cttagccgtt aaatttggat tgaccgtagc
     4621 tgatttaaaa gatactttag cgccttatct aacaatgtca gaaggcttaa agttagcagc
     4681 tctagccttt gataaagata tttcgaaatt atcttgttgt gcaggttaat atttctattt
     4741 agtttcttgt agtagctggt aacaccttgg tttatctttc gttattatct ataacgatag
     4801 gagttgaaga ttatgacatc agcaaacgtc tacgtgccaa ttaatagctc tattctagtg
     4861 gaggttgaag aaataaaaga ctatttagaa acgcaattgc tcatttaggc aacgcagcaa
     4921 gatattattg aagatgcgat tgttgcctta cgtgcttaat atagataatt aaatcgtatc
     4981 aaaaaaggat tctaccgctt aaaggggtac cgtcaggaaa atgcggattt acaacgttaa
     5041 ggaaatttat taaagagacc aattcctcag cactaaaatt gataattggg ctttctttgt
     5101 tactatagtg ttaggtaagc tttttacttt atcaccggtt ttcccctcac ttcacaccta
     5161 cgggcgagtt tccccacatc ccgttttcca tctaatccaa ttcattcaat atctttatgt
     5221 gtatgaaaga tcagattgct tgatttagac atcttacacg taatttattc ttaaatgggt
     5281 acggtaaacg agcaataata cgagcaaaag tatacctaca aaaataataa ctctgtctaa
     5341 ttttttttga ttcatatgct tattgcttaa accatataat ttcagtccaa gaaagcctcc
     5401 aacagtattt gtaattacat ctgttatgtc tgtcgctcca atagcgaaga taaattgaat
     5461 tatttcaaaa gtaagactta aaaccagtac aaaagcaaac ttaggtaaaa atccgatttc
     5521 tttaaaattg acattcaaaa gcaagccaaa tggaataaag attataacat tatctatcat
     5581 ctctctgaaa ttcccagtag cagtaaatgg agtcaagtta agacttcttt gatgataatt
     5641 aaatactgat aaaatattgt attgtaattt gaataacact aaccagatta gtgtcactaa
     5701 atataaagct agcaatcctc tagataatat ttttcccaat ataatttcct ctaattcttt
     5761 tgtttaagtt gtatcattag tatagcaatg tcctagttaa cgtgggtttt aaattaaaaa
     5821 gctaacgtta ccccaaaagt gctagtacaa taatgctgtt cattaatttc gttggtttca
     5881 ttccgccatc cttacctcct tgaattagta tgtgttgatc cgggaaacac tgtcacaatt
     5941 acaccgtcta tattgtttcc tgatatctca taacgaagat tagtcggcac atgaatggtg
     6001 gtatttttag taacaggata ataaaagatc tcatattttt ccccatttac actaacagaa
     6061 atggtttttt cttcttttag gtaatccata tattcctcga gaacgaaatt cttttctttc
     6121 ataatcgcac tatgtggtaa accaacatag cgaatatgcc atggttcata ttgaattcct
     6181 gttaactctg ttttgtcctc tggataacgt aaaatgaacc cgtatttcca agcattttct
     6241 tctatccact ttccttcagg ggctcgttcc attttcgtca agcttgatcc tacatctagt
     6301 gataaacctg aattatgctc actataacct gctggtaagg catactcagc ccccatttct
     6361 tggtaaagca cactttgctc atcaaagtct cgatagccac tattaataat aaaatgacta
     6421 acgccaccct ttacagcatc attgaccatc tctgaaaatt tttgtgctat ttcttttgac
     6481 atataaatat tactatcaag caacccgtat ccatttatta attcgtcatg tttagataaa
     6541 ttcacgatat ctgacttcac actttcttgg cgaacaggat atttactatt gattaatagc
     6601 agatttcctt gataaacctg ttcttctgta attgttttct cttgggtatt ttcagaagtc
     6661 ccactatttt ctaaatgttc tttgggattt tgatcataat tttgaaattc gactttttcc
     6721 tgagaaaaca gtgcttcatt aacgtagtca taacctaagt atattaagaa taacaataac
     6781 aataaaaaaa acaacttctt cattttcagt ctcctcctta actatatagt tggaagtatg
     6841 aaatatttct agaatcttcc ccttaaacgg cacaatctcg tttgagattg tgccgttttg
     6901 ccccgtaaat tacagtcaat tatttcgccc tactggattt actattatca ccaa
//
LOCUS       DAZUKC010000116         6941 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000116 DAZUKC010000000
VERSION     DAZUKC010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.118"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            678..872
                     /locus_tag="ACG80T_002507"
     CDS             678..872
                     /locus_tag="ACG80T_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842121.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            1063..1312
                     /locus_tag="ACG80T_002508"
                     /pseudo
     CDS             1063..1312
                     /locus_tag="ACG80T_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1556..2386)
                     /locus_tag="ACG80T_002509"
     CDS             complement(1556..2386)
                     /locus_tag="ACG80T_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFS4842122.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            2594..3928
                     /locus_tag="ACG80T_002510"
     CDS             2594..3928
                     /locus_tag="ACG80T_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4842123.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKIFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            3969..4880
                     /locus_tag="ACG80T_002511"
     CDS             3969..4880
                     /locus_tag="ACG80T_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4842124.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            4942..5730
                     /locus_tag="ACG80T_002512"
     CDS             4942..5730
                     /locus_tag="ACG80T_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4842125.1"
                     /translation="MTVIGIILIIPLLWMVFTSLKPMEEIVRYPPTFFPEKIVWENYL
                     DTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYGFAKLDFPGKKLMFALVLSTMMI
                     PGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFNIFLMRQFYLSINNELIEAAEID
                     GANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFLGPLLYIQDQEKYTLQIGLQVFQ
                     NQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGMDLTGGSKG"
     gene            5727..6344
                     /locus_tag="ACG80T_002513"
     CDS             5727..6344
                     /locus_tag="ACG80T_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFS4842126.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            6346..6864
                     /locus_tag="ACG80T_002514"
     CDS             6346..6864
                     /locus_tag="ACG80T_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFS4842127.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
BASE COUNT         2079 a         1201 c         1392 g         2269 t
ORIGIN      
        1 ctaaaggatt cctaaccatc ttattagctt ttatagggta tatgttggtt gggctgctaa
       61 aatcatacag taatgagttg ctgaattttt caacatttat taatgataca ttagttccaa
      121 gtttgttttt cattgtattt tttgctgttg gttattttat aataaagata tgatgactaa
      181 tttagctcaa ctattccagc gtcagctgaa gatattgcaa ttcccgattc cttaatcatt
      241 tatctggatg agacagaaag tgaagttgca acagatgtgc atgtagttga ggataaaaat
      301 gatagtttaa aaattaatag ttcagatact ttactaagag taagtcctat cttttcagct
      361 gttatttaga tgcaagtagc aactaagtta ggtagtactc ctgcaatgga taggtatttg
      421 tgtgttcgat atataagtgg tcgaagtatc aaggttatgt atactggatg ggagatagaa
      481 gagttaaaaa ttggaggtca ccgccacaaa tggaatttag atttagaggt accttagatt
      541 tggtatagtt attaaacaca agatggcctg gtttgtaggg aaagtcgaag agtatttttc
      601 cacttctgcg cccctttaat ttttctagta atgccgtgaa tttgtgatta attggaaata
      661 tgaagaaagg agagtctatg aaaacaacac tagttctatt ctataaaaag catccttatt
      721 ttacacttct gataaatata ttgttggcta gtgttatcgg gatttctgtt gaatatctaa
      781 taaataaaga tttcataggt tcttgttttt atacggcttt atttcttgga cttcttgaag
      841 cattttccat ttataaaaaa tcaaaaaaat aaaatagatt gccgtaagag atctttttca
      901 tttcattttt ttctgaaaac ccgttgtttc tgtcagtgaa gcagcgggtt tttagattta
      961 ttgtctttag acctaattga gcgcgccgag cgattgaaat acaaaaccac ttgctatgcg
     1021 ggtggagcaa taaagggaaa tagtttagcc cgtacaaagt agatgtgtaa ataccacatt
     1081 gtattcacac caaagtatag aagaaaaata atttataatc aatatcgagc caatattcag
     1141 gagatcatca agagattgtg caaatctaaa atataaattt gggtatcgac acttttgggt
     1201 ggaaggatac tatgtgacaa aagtaggaca aaatgaagcg accatcaaaa agtacattca
     1261 agaacaagaa aaacatgata gtgcgttaga taagctgagt gtacgagagt aagacgatcc
     1321 ttttaagggc taaggagtag tacataaacc ttttaagagg tgggcaaaac tggcaaaggc
     1381 aatatggctt gaacataaaa tatgaaagcc agcgtcttta ggcgctggcc ggtgatttga
     1441 gcttatagct ctagagaaaa ccaccctttt gaagggtggt tatgattaat gactttagtc
     1501 agttttgctc acaaagcgtg tggaacaaaa aacgggtgtt tctacgggta gatatttatt
     1561 tcttgaatgg aggtgtccca tacatttttg gtgttactgg ttctaatttt ggtgcagcgc
     1621 cttcgatctt aggttctttc aaccattgat attcgccttc gccatccagt gctttgccac
     1681 tagcccaaga accttcacta ctttggtctc cctctgagaa gttatagaag tcataagcaa
     1741 tgtcttttcg ttctttgtca cgcgggaaag tacttgggac taagattgaa ccttcttttt
     1801 cttctaattc tttcactgca cattcccata gattttgatg atagctatct cttgcaagga
     1861 gaacagacag catatctttt acgccacgat catcgatcat ttcgtaaata cgtgctactt
     1921 gaagtcttcc ttgtgattcg gcatttaaat tagctctaaa gtctgctaac aagttgccac
     1981 tcgcaatgat ataggcaccg ctccatggat tgcccacgct gtcagcagga cgaggtccta
     2041 agccagctac gatcgcgtgc tgtggattca ttccagagat aatggcaccg attgttggat
     2101 ctttttgcat cgctgcttct tgatcttcaa caggcgcacc ctctaataaa cgagcaacca
     2161 ttgtcgccaa catttccacg tggccgattt cttctgttgc aatgtccatc agcatatctt
     2221 tatatttttc ttctcctcgg cagctccagc cttggaacag gtactgcata gctacactca
     2281 tctcgccata ttggccgccg atcagctcct gcagatattt cgcgattaat ggattcggac
     2341 gatctggttt tgcttcaaac tgcaattctt tctggtgtct aaacatcaaa attcctccta
     2401 atctattttt aatacattct cattctgcta cgaaagatta aaaaattcaa agaaagcgct
     2461 cgcgaattat ataaagtatt ggtatcatta ataatttttt tagaatcata ccttataaat
     2521 tgttattttc agaacaaaat gatatcgtta tattaaaacc ggcatcggtt acattaaaat
     2581 ttggaggtaa aatatgaaaa aattattgtt tacagggttg ctggcaagtt cagcattgtt
     2641 tttattagga ggatgcggat caagtaatgc aagcgatgcc agttcaagtg cagcaaattc
     2701 ttctgaaaaa acaagtgatg gaagaaaaaa tattgatttc tggtcctttt ggggatccgg
     2761 tgcacgaaaa gaagctatcg aagaaatcat tgaggatttc aatcaatcac aaaacgagat
     2821 ccatgtaaat tataagtatc agccatgggg tgacatctgg actaaatctt tatctgcaat
     2881 aacagctgga aatgcacccg atgttattgt acaggacata aattccgttg cccaaagagc
     2941 agaagcacag caagcaacta atctctccaa atatgtagaa aaagacactt ctaaggattt
     3001 ttatccgcag ctttgggata cagtagaata tgaaggtgat ccttatgcga tcccattcaa
     3061 tacagataca caagtcattt tttataataa aaagatattt aaagaagctg gaatcagtga
     3121 acaagatcta ccgacaactt gggatgaatt agaagaaaca gcacgtaaac tagataaaaa
     3181 agaaggaaaa gaatttacac gcatcggttt ttatccatta tggaatttag gggctgatgt
     3241 atgggcgcta aacgcagata acgggactag ttggtttgac aaagatgaaa aagtcaagat
     3301 tgatactaaa aataaagtgg aagccttgga gtggatccaa gactggcaag attattatgg
     3361 gaaaaatacg atcaataaat tagaagccga atttgggtct ggtgtatctg atccatttat
     3421 ttccggatta gtagccatgc gtgcccaaaa tatcaattat tactctagtt tgatggaaaa
     3481 tgcaccggat gattttgatt ttggcgtgat gcagctccca gaaaaagaag aaggttcagg
     3541 ccattggtcg tggggtggcg gattcgtctt ggaagtacca tacggagcaa aagatcctga
     3601 ggcctcttat gaattcatca aatatctttc ttctccagaa gtacaagaaa aattcggcga
     3661 aaaaagtttt gatatcatgg cgagcaagac agccaatgag aatttagtga ataatgacaa
     3721 cctaacggat aaaggaaaaa tgatctatca aatggcggac gaaaacttta aaaatacggt
     3781 gatcacacca gttcctttag atgctccgga ttacacgaca ttagtgaatg aacagattga
     3841 ccagattctt ttaggaacaa aatcaccaaa agaaggattg gcagatgcac aaaaagcagt
     3901 agaaaactta gtcaagcaaa atcaataatc catttttgac tgtctaggta cagaggaggc
     3961 aagaaaattt gaagcgttta tctatccgaa agaaaaaaga agcaagatgg gcctatttat
     4021 ttatctcgcc ttttattatc gggttcttgg tatttatgct agggcctatg cttttttcag
     4081 ttattggtag tttcaccgat tataatttga catccaagat gaatttcatt ggtctagcaa
     4141 atttcaaaag aatgttcgtt cacgatgatt tattttggaa atcgttatac aacaccgttt
     4201 atttcgtcat cttcaatgta ccgctgacga cgattttcag tgtgttttta gccacagtac
     4261 tcaatcaaaa aatcaaagga atcagtgtat tccgtacagc tttctattta ccggcagtct
     4321 tatcaggagt agcagtctac atcttatgga tgcagctgct ttctccatct gcaggattga
     4381 tcaacacatt tctgggctgg ttccatatcc ctggaccagc ttggctgttt gatccacaat
     4441 ggacgaaacc tgcgttgatt ttgatgaaaa tatggagttc tggaggagca atgctacttt
     4501 acttagctac attacaaaat gttcctagat ctttatatga atcagcagaa atcgatggag
     4561 caggcgtttg gggaaagttc aaaagtatta ctttgccatt aatcactcct attatttttt
     4621 acgatgtgat cacctctacg atcggttctt tccaaatttt tcaagaagct tatgtcatga
     4681 cgaaaaatgg aacaggcgga cctgcaaatt ctttgctttt ttataatctg catatgtgga
     4741 acaaggcgtt cgtagctttt gatatgggtt atgcgatggc tatgtcaatg atcctgtttc
     4801 tgatcgtgtt ggttctgacg tttgtcaatt tgaagcttgc acctaagtgg gtctattatt
     4861 ctggaggtga cggcaaatga tggattattt caaaaagacc agtgtgaatc ggcaaacaaa
     4921 aaaatactaa gttatgtcct catgacagta ataggaatca ttttgatcat tccattgctt
     4981 tggatggtgt ttacttcatt gaaaccaatg gaagaaatcg ttcgttatcc tccgaccttt
     5041 ttcccagaaa aaatcgtatg ggaaaactat ctggatacga ttgccgcctt tcctttttgg
     5101 cgctatgcaa gaaatacatt attcatcact gtgctagtag tgatcggtaa tgtcctttca
     5161 aattctttta ttgcttatgg atttgcgaag cttgattttc ctgggaaaaa gctgatgttt
     5221 gctttggttc tttctacgat gatgatcccg gggtttgtaa cgatgatccc tcaatatgta
     5281 ttattctcca agatcggctg ggtaggtact tatttgcccc tgatcgttcc atcgttcttc
     5341 ggcaatgcgt tcaatatatt tctgatgcgt caattttact tatcgatcaa taatgaattg
     5401 atcgaagctg ctgaaatcga cggagcgaat catttgtata tttggagtca tttgatgctt
     5461 ccattgacga aacctgcctt gattaccatt gcgatcaatt ccttcaacgc agcttggaat
     5521 gatttcttag gtcctctgtt atatatccaa gatcaagaaa aatacacgtt acagattggc
     5581 ttgcaagttt ttcagaacca agcaacgaca caatggaatt atctgatggc aggagcaaca
     5641 cttgtcttga tcccgacgat tttgctgttc ttttttgcac aacgttattt cattgaaggg
     5701 atggatttga caggaggttc aaaaggatga tgaatcgatc gattggtaaa aaagccaatg
     5761 attatttctt agaaatcatt gaatggattc cacgtcttat ccatttacag ctactatggt
     5821 tcctgtgcgt cttgcctgtt ttcacattag gaactgcaag tcgaacagtt ctgtatatga
     5881 tttatcatta caaaaaaaat gaagagaaaa agctcggttc actattctgg aaaaagtttc
     5941 gtgagaactt ctctctttat ggaaagcaag atagtttagc aagtgtttat tttcttcttc
     6001 tattgatcga ttatcagatt ttccgctatt ggggtggggg aattggctat acattgatgt
     6061 atactacgtt gtttttgctt ctattaagcg cgcttctctt tagttacaaa atcttgcttc
     6121 aactggaaca aaaagaagct tcttggatta ctgcattttt cttgtttttt aatgatttca
     6181 aaagcgcgct tttctatttg gcaggcacgt tgatccttct tgtcgcattc tggtttgcag
     6241 gaccaatcta ttttgcttta ttaggtttca gtttcttgtt ttattttcaa gtcctgcttt
     6301 ttatccatcg tacgcaagaa aaggttctta aaaaggagga ataacatgtt gcgtttgatt
     6361 gctaaaatcg atgatagtat tatcaacaag ttcgccaagc aacggaaaac ggatagtcga
     6421 aaaatcaatt ttttgaaatt tatcagtaat atctctaatg gtggttttat ttggttgctc
     6481 cttgctgctg ttttagtctt gatacctgat actagagaat atggtctagc cattcttatc
     6541 ggcagtttga tctatttttt tgttggtttg ctttttatca aaaaaacgac aagacggaaa
     6601 agaccttatg aaaaaaatcg tcggcttgtc ctttacacaa gtaaaccgaa gacttcttct
     6661 tttccttcag gacatacagc gctagccttt ttatcagtag ctgcgttgtt cgaattaagt
     6721 ccagttgcag gaatattagc ctttttattt gcttgtttga tcgcttattc gaggattttt
     6781 ttacttttac attatcctac tgacatttta ggaggagcta ttttaggaag tgttattggc
     6841 atcctatgcg ctgccgttat ctgaattaat tgtacgaata aagcatgagt gcgtaaatgc
     6901 gcatttgtgc tttatttttt tgtttcgagg aaagcgtagt t
//
LOCUS       DAZUKC010000117         6805 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000117 DAZUKC010000000
VERSION     DAZUKC010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.119"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..395
                     /locus_tag="ACG80T_002515"
     CDS             <1..395
                     /locus_tag="ACG80T_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842128.1"
                     /translation="LLNLDSVNSNAQIKDQRVAEHNNKKMSLTKNIYSMSDEELYKEF
                     GDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSELVSYKQNHRQFIDVNTDSNAI
                     SIKDLKELLNSKRNELKQIKETNSVELE"
     gene            409..1098
                     /locus_tag="ACG80T_002516"
     CDS             409..1098
                     /locus_tag="ACG80T_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842129.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1223..3574
                     /locus_tag="ACG80T_002517"
     CDS             1223..3574
                     /locus_tag="ACG80T_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFS4842130.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3654..4049
                     /locus_tag="ACG80T_002518"
     CDS             3654..4049
                     /locus_tag="ACG80T_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842131.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4374..4991
                     /locus_tag="ACG80T_002519"
     CDS             4374..4991
                     /locus_tag="ACG80T_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4842132.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5032..5331
                     /locus_tag="ACG80T_002520"
     CDS             5032..5331
                     /locus_tag="ACG80T_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFS4842133.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5365..5532
                     /locus_tag="ACG80T_002521"
     CDS             5365..5532
                     /locus_tag="ACG80T_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS4842134.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKKYFYCP
                     KCHRRYGVHGT"
     gene            complement(5577..6047)
                     /locus_tag="ACG80T_002522"
     CDS             complement(5577..6047)
                     /locus_tag="ACG80T_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4842135.1"
                     /translation="MEFREGAFDFQTSQQNPSLDRLGRDYEDIKNTVAFMKQKKVALK
                     ILDAQFLDFNTGNELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKG
                     RPIEYSINSTDPQKRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
     gene            complement(6053..6733)
                     /locus_tag="ACG80T_002523"
     CDS             complement(6053..6733)
                     /locus_tag="ACG80T_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
                     /protein_id="HFS4842136.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWVQEYSKVLYYLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVK
                     YLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEI
                     KVLMGITA"
BASE COUNT         2290 a          993 c         1349 g         2173 t
ORIGIN      
        1 ttcttttgaa tttagattct gttaattcaa acgctcaaat aaaagatcaa agagtagctg
       61 aacataataa taaaaaaatg agtttgacca aaaatatcta ttctatgtca gatgaagaat
      121 tgtataaaga atttggtgat ttatccgggg aacagttaga agaaagtatt ccaggaatta
      181 gtgactcaga taaaattgtt gtcttgtctg ctggtgggca attaatggga gatggcaaat
      241 ccgaattagt tagttataaa caaaatcaca gacagtttat tgatgttaat actgattcca
      301 acgctatttc gattaaggat ctaaaagagc ttttaaatag taaaagaaat gaattaaagc
      361 agataaaaga aactaattca gtggaattag agtaaaggaa gattcattat gaaaaagatt
      421 tttggtgttt ctgttgggct acttatgatg ttaacatttg gaaatgcagt atatgctgct
      481 gagaaagaag ttatttcaga tccagagcag tacgcctcac gatactttgc tgattttgaa
      541 gaagaagata aagtacttgc attgccagaa ggtggttttt taaaagggga agctaaaatt
      601 gtatctatgg ataatccaga tataattcaa ggttcatata attcagaaac tgatcctgct
      661 tcagtttcag tgacagaagc aaaaaaaatt gttgctgaaa aattcaaaaa tgatattgaa
      721 actactccaa ttatgcctcg aggagcattt ccacccgatg ttggtactag cggacacctt
      781 cttgtactag caggaggagg aagctataaa tcacagccat ttagtggtgc gggatggcgt
      841 tttggaggta tgggtgtaaa accagctgat ggtacaggca actttttacg ttggaaatcc
      901 tacattgacg atggaagagt aggaaatgta aatcaagcca acgcaactaa atctggttct
      961 attcaaggaa tggcattgta tgctcgtgaa ggattcaaat actttgcacc aaacgggcct
     1021 ggttggacaa tgaatatagg tatttattac acttataatc ctgttgcagg tacgtattat
     1081 gttgttcaaa atatttaata aaacagcaca aagagatttc tttgtgctgt tttattattt
     1141 ttagttgttg acaaacacta aaatataaaa tataatcaac acataaacga acgtatgttc
     1201 ggcaagaaag caggggattt ttatggatga gatggtttta agtactcaga aatggctaaa
     1261 caagaaatac agtaatgtta cagggtttga taaggttcca gaaaatgggc gaacagggtg
     1321 gcctacaatc tatggactta ttgaaggact acaagtagaa cttggtatta ctaatctagt
     1381 agctaatttt ggacctacca ctgaaaaaat gtacgataat caggttactc ctaaatgggg
     1441 caaaaatcta cctaaaaata ttgtatttct tattcaaggt gccttttggt gtaaggggat
     1501 taatccaggt ggcttcgatg gggtatatac tccatattta gatactgctg taaaagagtt
     1561 gcagactgat gcagggttta atggaagcgc tgtaactgga gttttggatg cgaaatgggc
     1621 caaagcgtta tttgatatga gtgcttttgt tcttgttccc ggcggagata gtaaaattag
     1681 aaaaatgcag caatggttaa atgtaaatta tttagaatac acgggaataa tgccttgtga
     1741 tggtatttat caaagaaatt caaatcaagc attaattttt gcattacaag ctgaactagg
     1801 atattctccc agtgaagcaa cagggtctta tggtaatggg acaactagta aaacttatcc
     1861 tgtgtctgaa ggtaactcgg gaaactatgt aagaataatt caatatggat tatatgttaa
     1921 tggttatttt gaagacggta cattcgatgg tattttcact aaatatatgg gtttagaagt
     1981 actggcattc cgaaaattta tggtattgcc tgaatatact gaaatagcag atgttgttgt
     2041 aataaaagga ctattgtcta gcgctggaga tattagaaga tctgcagatg gcgcagatac
     2101 ttctacgcaa ttaactagat ctcaaatcca aacattggtt gataatgata taaaaatagt
     2161 tggtagatat cttactggta ctgttggaat tggaaaagat gaacgaaata agtatctaac
     2221 tacagaggaa ttgaataata ttttttctaa aaatttatct gtgtttccta tctatcaaga
     2281 tggaggtgca gcattagctt attttactta tgatcgtggc ttatcagatg gtaagaaggc
     2341 tatagatgca gcaaaaaatc taaatatacc attaacaaca gttatttatt ttgctgttga
     2401 tctagacatg ttaggggaag aaattcttgc atatgctgga gaatatttta agggggtatc
     2461 tgctgtaatg tcatatagcg ggtatcaaac gggtgtttac ggaacaagaa atgtttgttc
     2521 tcaaataatc aataacgggt atgcttcgtt tgcatttgtt tcagatatgt cgactggtta
     2581 tagtgggaat ttaggttttc ctatgcctag acaatggtct tttgatcaat ttgttgaatt
     2641 taccatagga agtggcaacg gagctgttgg tatcgattta atcgctacat cgggtagaga
     2701 tagcggattt aatgagttat caaatgatac taataatgat aattacattg ccaaatacaa
     2761 tcagaaggtt ataaatcaga tggtacgtgc gtatcaatat ctatcacaag ctggtcttga
     2821 taatccttgg aaccctcatt tgacatttta tagatatgta aattattctg gtttgtcatg
     2881 ggacatcatt tcttctcctg taacagagca tgatagaaaa atttatgatg aatataggaa
     2941 taaattaact aatagcgagg gcctgtataa ttattttata gatcctaata ctggttcaat
     3001 aataggtttg cctcacttaa ttgtgacgtt acaatcacag atgtttctag ccgacacaat
     3061 caatgatagt gtttctgact ttgcaggttg gttaggagat ttaatgactt gctggggtga
     3121 agttaagaaa ttaggaatac aaatggagca aggtgtattt gcattagtag gtacggctgc
     3181 aggtggaaca tttagtctag aagatttata ccaagatgtt gacgcggtaa atgtttatac
     3241 cttagcaaaa agaaattctg attcaacagc tttatcggct ttaaaaacgt actattgtga
     3301 taagggtgct attgatagat ttaatcaatt tgttaagaac agatttggtt cttcagataa
     3361 aatcgcttca gtaacaaata gtttattaga aaatgacgaa acagttgata tcttaggtgt
     3421 agggataatt tcatttttca ttgataaaat agttaataat tcacttggga cagattttac
     3481 ttatcttgat tttattggag atgaggggaa agttgcaaat ggattttctg aaaaaattat
     3541 tcaatttcaa gaaaatagaa gtaaacttaa ttaagaatcg tttactattt aaacagtgtc
     3601 cttttccttt ttaaatatat acgataaaat aagtatatga attgtaaaag gacatgatga
     3661 ttatggaaga aatagaatgg aataatctga ctcaaaataa taaaaataat tctggatgtg
     3721 gaattatctt tatgtttgtt atagttttgg gaatagtagt acttttaata actgtcaaga
     3781 tagtagatta ctttgcgaat actcctgaag aacaaataca gagaaaactt cctgagtttg
     3841 aaagagtacc accttatgat ccaagagata attcatttca agtaaaaaaa ttaaaagagt
     3901 ctccaaatga aatgtggttt gaagttacag ttgaatcaag cgacttcaaa gaaaaaaaga
     3961 aagaatacaa ttatattgta aaggcaaaag tatcaaaaag atttattttt aaccctaaat
     4021 tagaaatata tagtgttgta atagaataat agtgttaaaa aaattgtaaa agttaaagaa
     4081 gtcaaattct gatgtgaaaa ataaaatata ccatggaaga cataaaagaa aaactggata
     4141 actagtttat ttaatggcag accgttaagg cctgccattt ttatgtattt tagcggaata
     4201 aatataatca tgcgggagtg taaacttact gtttcaaaaa gtatactttt tgacatgcct
     4261 tgattttctt tgtttatgta tggcataata accacataat acaatttgaa acgtttctga
     4321 tttaatctga ttgttttgaa acaattttag tgaaaaaagg gtagataaaa acgatgagaa
     4381 aattttttta tatccgggtc agtagttatg atcaatcgat cgaacgacaa ctgatttccg
     4441 cgaaggaatt aggaatccct gaagaatata tatttgtaga aaaagccagc ggcaaagatt
     4501 ttaaacgccc tgaatatcaa ttaatgaaac ggatgtttcg aaaaggagat ttactctatc
     4561 tacaatcatt agatagacta ggcagaaata aacaaatgat tttagaagaa tggcgagaat
     4621 taactaagga aaagcagatt gatatttctg tgttagacat gcctctgtta gatactgcta
     4681 gttacaagca actagatggg ttagagacgc ttatctctga cttaattctg caattactca
     4741 gctatatggc cgaagacgag cggaaacgaa ttaatagccg gcaaagagaa ggcgtagaag
     4801 ctgcgaaaca aaagggtgta aaattcggca gaaagaaaat tgctttacct cctgaatttc
     4861 ctgcgatcta ttctgactgg aaagcaggaa agataactgc agtctatgct atggatcaat
     4921 tgggcatgaa acgaaatacc ttttatcgac gggtgaaaga atatgaacat accttgcacc
     4981 acttttcttg atagatataa cagctattag acgggaaggg agagtttgaa aatggactta
     5041 acgaatcaaa gggtcgtttt tactggaacg ctgcagcaaa tgacacgaac ccaagccatc
     5101 ggtttggttc actctttaaa cggccattgt caatcaagtg ttacttcaaa aacaaatttc
     5161 ctggtttgtg gtcagtattc taagtcgttg tttgatgatt ctctctatac aaaaaaggaa
     5221 caaaccgcgc gggacttgat tgcgttaggt catgagatta ccattttatc agaagtcgaa
     5281 ttttatgcgt taattagtca acagttgaaa gaatggcaac ggtatttata atcgttagta
     5341 aaatttacaa ggaggaaaat ttttatgaag cgaaaatctt atgatcgttg gggcattgaa
     5401 ataactagtt ttaatcgaaa tattgctttt cacgtggagt gtgactgtgg ggaattagca
     5461 aaaagtgttt atcaaaaaaa atacttttac tgtcctaaat gccatcgccg atacggggta
     5521 catggaacat agaaacgaaa tagtgcggta gaaaagcgaa tgaatgtagt atatttctac
     5581 aaatcattcg ctttctttat tttataaata gttggtcgag agatcccgtt ttcttttgca
     5641 atctctgcaa ctggaatgcc ttgctttaac atgtccacca cggttttaaa aactaagcgt
     5701 ttttgcggat ctgttgagtt aatagaatac tcgattggtc gtcccttata tcggccggct
     5761 ttttgagcca ataatactcc ttgccgctga cgttcacgaa ttttctcacg ctcgttttgt
     5821 gcaatataac ttagcagtga taaaaacata tcgaacatag cggtatccag gagttcattt
     5881 ccagtattaa aatctaaaaa ctgtgcatct aatattttta acgctacttt tttctgtttc
     5941 ataaaagcaa ctgtattttt tatatcttca taatctcttc ctaaacgatc tagtgatggg
     6001 ttctgttgcg aagtttgaaa atcaaacgcg ccctctctga actccaaata tcttaggctg
     6061 ttattcccat taataccttg atttcagtag acaccgaaaa gccgaagagc gttccatttc
     6121 ttcggttctt tttatatatt cctcgaatgg tctccatgcc cttaatcgtg gaagaggctg
     6181 tacggagact ttgataaaat ttatttcgtc gtttaatagg tcgatggtct tgttctatta
     6241 aattgttaag atacttcaca gttcggtgct ctgtcttagt atataaaccc acactctgta
     6301 actttctaaa ggcggagcca agagaaggtg ctttatcggt cacaattgct ttcggctcac
     6361 caaactgttt atggagtcgt tttaagaaag cataggctgc ttgcgtatcc cgtttctttc
     6421 gtaaccagat atctaaggtt aagccgtccg catcaattgc acgataaaga taatgccaac
     6481 gtcccttaat tttgatatag gtttcgtcca ttttccatga atagaaggat tgtctatttt
     6541 tcttcttcca aagataatag aggactttgc tgtactcttg cacccaacga taaatcgtag
     6601 tatgacaaac atttattcca cgatcatata acaattcctg aacttcacga tagcttagat
     6661 tgtaacgcag gtagtaacca acagcgacaa taatgacgtc ttttttgaat tgtttgcctt
     6721 taaaatgatt cattactctg tcctctctgt cttttttctc aattttacac taaaatagat
     6781 tttttggaaa actttgcaac agaac
//
LOCUS       DAZUKC010000118         6703 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000118 DAZUKC010000000
VERSION     DAZUKC010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.120"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            54..626
                     /locus_tag="ACG80T_002524"
     CDS             54..626
                     /locus_tag="ACG80T_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFS4842137.1"
                     /translation="MTENKATQERTPQERLQEEQEHKEHVHHTKINAAAIADHLLGNM
                     HTLHVKLHQYHWYVKGANFFTLHEKFEELYNENEKWFDKLAERLITSGHKPASTTVEF
                     EKYSMLSEDAVNKYAKAEDMVENIIEDFRSTRDLTIRAIRLAQDEGDDALEDTLIAFK
                     NDLDKNIWMLQAFIGKEALEDDDALDEDED"
     gene            771..1475
                     /locus_tag="ACG80T_002525"
     CDS             771..1475
                     /locus_tag="ACG80T_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011476586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS4842138.1"
                     /translation="MTKHTHHEIGDHAACIRLVPIFNHLEDSAMNYIAEKAHTKQYQK
                     GEFLFRSQEKEDTLYIINRGKIRIYRLADSGKEQLVRILNPGDFTGEWTLFNPDAVHE
                     EYAEATRDTVVCMIQQKDVQDFLEQYPAISLKLLAEMSKRLESSERQTTQVAVESVIT
                     RLVLFLAENVEPEMGNSPTITLPMAKKDIASYLGTTPETISRKFGELEDEGLIQQLSG
                     KRIKIQDLDDLLLYSE"
     gene            1703..2041
                     /locus_tag="ACG80T_002526"
     CDS             1703..2041
                     /locus_tag="ACG80T_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842139.1"
                     /translation="MHCFWVIVAITLSAAGAFFVVPFTKNTIGGEAKTSNVVEADRLA
                     MEKEEEVVKGAGVIGHGKVAYYEASDQGDKSVFTVWFPWSLAGILFNITMTRMTRKIE
                     EMEQIEWDSY"
     gene            2026..2880
                     /gene="lgt"
                     /locus_tag="ACG80T_002527"
     CDS             2026..2880
                     /gene="lgt"
                     /locus_tag="ACG80T_002527"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFS4842140.1"
                     /translation="MGFVLGVLNPHVVEIGSVVIHWYGVIIAAGILAALQLSTKEAKK
                     KGLEEDTIIDMALWAIPIGLLGARLYYVLFELGYYLQNPGQILAIWNGGIAIYGGLIA
                     GGGTLYWYAKKKGTSLALVLDILAPNVLLAQSIGRWGNFMNQEAHGGPVTRQFLENLY
                     LPEFIIEQMNMNGTYYHPTFLYESLWSLLGFVLIVTIRNRKQLLRQGEVALSYVLWYS
                     VGRFFIEGMRTDSLWIGEWIRVSQALSLALFIGAIVVWFIRRQDYPPISYYSDGNKGQ
                     KKTIKMVN"
     gene            3027..3260
                     /locus_tag="ACG80T_002528"
     CDS             3027..3260
                     /locus_tag="ACG80T_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFS4842141.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGIDKDSIKVLFNSSKV
                     KTNFDSAVTSIEEIQKAIENVGYPVLKAKVKAV"
     gene            3458..5362
                     /locus_tag="ACG80T_002529"
     CDS             3458..5362
                     /locus_tag="ACG80T_002529"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS4842142.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTEMAPESALKQMDNGEFEEVEVDDIDEGDILLVKTGAKVPVDGIVL
                     TGEGHINEASITGEAIPVNKLADAEVFAGTILENGTIQIRADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLAIVVWAFSQNIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVIQDFSNVDTIVFDKTGTLTVGNPTVAATELYEKDPA
                     ETLGYLASVERESDHPLAKAVLNQIGETNFYPVEGTEVVKGGGIVSSVAGHRVAVGNV
                     ALMEKENVTLSKKVQKDVKRFEQQGNSLVLTAVDGELKVLMGIRDQVRPGVKANLQEL
                     KDLGVKNLVVLSGDNQGTVDVVAGELGLTEAHGYMLPEDKSAYIGKLQQRGQIVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISVGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPTEKVKIS"
     gene            5716..6081
                     /locus_tag="ACG80T_002530"
     CDS             5716..6081
                     /locus_tag="ACG80T_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HFS4842143.1"
                     /translation="MTAEVCTTTCIHKEKVASVKNNLKDKDLSTLLVLGKCFSDSSRI
                     KIFYALETYKEMCVCDLAAILNASIATTSHHLRFLKKNGMAKSRQYGKVVYYSLANED
                     ILSAIHVFLKLSENLSMKS"
     gene            6098..6703
                     /locus_tag="ACG80T_002531"
                     /pseudo
     CDS             6098..6703
                     /locus_tag="ACG80T_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177616.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
BASE COUNT         2132 a         1101 c         1485 g         1985 t
ORIGIN      
        1 aggaatcaaa gaaagctatt ttaaacaaac caaaattata ggaggaataa atcatgactg
       61 aaaataaagc tactcaagaa agaacacccc aagaaagatt gcaagaagaa caagaacaca
      121 aggaacatgt tcatcatacg aaaattaatg cagcggccat tgcggatcat cttttaggca
      181 acatgcatac attacatgtg aaactgcacc aatatcactg gtatgtaaaa ggagccaatt
      241 tctttacgtt gcatgaaaaa tttgaagagt tgtataacga aaatgaaaaa tggttcgaca
      301 aacttgcgga gcgtctaatt acttctgggc ataaacctgc ttcgacaacg gttgaatttg
      361 aaaaatattc gatgctttcg gaagatgcag ttaataaata cgctaaagca gaagacatgg
      421 ttgaaaatat tatcgaggac ttcagaagca ctcgtgacct aactattcgc gcgattcgtt
      481 tagctcaaga cgagggtgac gatgctttag aagatacatt gattgctttt aaaaatgact
      541 tagacaagaa catttggatg ctgcaagcgt tcattggtaa agaagcatta gaagatgatg
      601 acgctcttga tgaggatgaa gattaaggta aaagtagaag ctgatttagg gattggaatt
      661 ctttccagtc ctttttccat tttcttgatg tgcatcaatt tttttgtagt aagagactta
      721 tataataaaa gcagcgaata taaacaaaat aaataaaggg ggagaatgaa ttgactaaac
      781 atacccatca tgaaattggc gaccacgcag cctgtatccg cttggttcca atattcaatc
      841 acttagagga tagcgcgatg aattacattg ctgaaaaagc ccacacgaaa cagtatcaaa
      901 aaggagagtt tctgtttcgt tcacaagaaa aagaggatac tttatatatt atcaatcgtg
      961 ggaaaatccg tatttatcga ttggcagact ctggcaaaga gcagctagtg cggatcttga
     1021 accctggtga tttcaccgga gaatggacct tgttcaaccc ggatgcagta cacgaggaat
     1081 atgccgaagc aacccgagat accgttgtct gtatgattca gcaaaaagat gtccaagatt
     1141 ttctggaaca atacccagct atttctttaa aactattggc ggaaatgtct aaacggttag
     1201 aaagttcaga acgtcaaacg acacaagtag ccgtggagag tgtcattacc cgtttagttt
     1261 tgtttttggc agaaaatgtg gaacctgaaa tgggcaactc tcccaccatc accctgccga
     1321 tggcaaaaaa ggacattgct tcctatttag gaacgacacc tgagaccatc agtcgcaaat
     1381 ttggagaatt ggaggacgag ggattgattc aacagctgtc tgggaaaagg atcaagattc
     1441 aggatttaga tgatttattg ctttacagcg aataatcgga cacacttttt tctacacgtt
     1501 ggtgttctgt cgctcttcat taacaacggg acagaactca gtgcttttgc ttccatctgt
     1561 ccgtttttag cttttttaag acagaacgac cttcattcat tggaaaaata aaagcacaaa
     1621 catgataacc ctcaagtttt tttgttttat ttttctttaa gatagggttg tcgaacaaaa
     1681 cgtaaaaata agtagggtga atgtgcattg tttttgggtg atagttgcca tcacgttgtc
     1741 agcagcgggt gcattctttg tcgtcccttt caccaagaac acgattggcg gcgaagcaaa
     1801 aacgtctaac gttgttgagg cagataggtt agctatggaa aaagaagagg aagttgtaaa
     1861 aggagccggc gtaatcggac acggaaaagt ggcgtattac gaagcgagtg accagggaga
     1921 taaaagtgta ttcactgttt ggtttccatg gagcttagcc ggaatcttgt tcaacatcac
     1981 catgactaga atgacgcgaa aaattgaaga aatggagcaa atagaatggg attcgtatta
     2041 ggcgttttga atccccatgt ggtggagatt ggttctgttg tgattcattg gtatggagtc
     2101 attattgcag cgggcatatt agcggctctc cagttgagca ccaaggaagc aaagaaaaaa
     2161 ggactggaag aggataccat tatcgatatg gccttatggg caattccgat tggactcctc
     2221 ggcgctcggc tttattatgt gttatttgaa cttggttatt atctgcaaaa tccaggacaa
     2281 atccttgcga tttggaatgg cggcattgcg atttatgggg gattgattgc aggagggggt
     2341 acgctatatt ggtatgcaaa gaaaaaaggt acttctttgg cactcgttct tgatatttta
     2401 gcgcccaatg tattgttagc ccagtccatt ggtcgttggg gcaattttat gaatcaggaa
     2461 gctcatggtg ggccggttac tcgtcaattc ttggaaaatc tttatttacc tgaatttatc
     2521 attgaacaaa tgaatatgaa tggtacctac taccatccga cttttttata cgagtcattg
     2581 tggagtttgt tgggctttgt cctcatcgtc actatacgga accgaaaaca gcttttgcgt
     2641 caaggagaag tcgctttgag ttacgttttg tggtactcag tgggtcggtt cttcattgaa
     2701 ggtatgcgaa cggacagttt atggattggc gagtggatcc gcgtttcgca agccttgtct
     2761 ctggccctgt ttattggcgc gattgtagta tggtttatac gcagacaaga ttatccacca
     2821 atttcttatt attctgatgg caataagggg cagaaaaaga ctattaagat ggtgaattga
     2881 gcaaaaagat aaaaagaagt gcaaaacttg atatgcgtca attttcgttt gctaaaagtg
     2941 agctatacta cagttgtaaa gaggaaaagg cagtgttcat taatctgctg ccgataaaat
     3001 aaaaacacat aaaggagata ggaattatgg aaaaagctat attgcaatta gaaacgttgt
     3061 cttgtccaag ttgtatgcaa aaaattgaag gtgccgtgaa atcagttaac ggtattgata
     3121 aagacagcat taaagtatta ttcaactcca gcaaagtcaa aaccaatttt gattcagctg
     3181 tcacttcgat tgaagagatt caaaaagcta tcgaaaacgt aggctatccg gttcttaaag
     3241 caaaagtcaa ggccgtttaa cagattaaat gaactaggca attctggaag aaaagctatt
     3301 tttccaggat tgcttttttt gttgcaatcc gattcgaggt gcttagaaac gataaaagag
     3361 aactttctaa acttgatgtc agtcaattaa ttacttaggc aattatctta tactgtgttt
     3421 atcaataaaa gagaaattaa atttggaggg aaaaagcatg caacaatata tattaagcaa
     3481 gaaaaatgtt atcaccgtca tcagtggatt gttaatcgta ctcggtttct tcagtcactt
     3541 tgttttagaa aacgtaggac tttcagagtg gtctttgatc atcgcctctg tctttgggat
     3601 tacgccaatt gccattcaag cgtttcaagc aatgaaagtc aaagtcatca gtattgatgt
     3661 cttagtcagt attgcagcga ttggtgcgct ttttattcaa aattatgaag aatcggctat
     3721 tgtcacgttc ttattcttat tcggacacta cttggaacaa cgaacattga accaaacgcg
     3781 atctgctatc aaagaattga ctgaaatggc acccgaaagc gccttgaaac aaatggataa
     3841 tggcgaattt gaagaagtag aagtggatga tatagatgaa ggggatatct tgctcgttaa
     3901 aacgggtgcg aaagttccag tagatggcat agtccttacg ggtgaagggc atatcaatga
     3961 agctagcatt acaggtgaag ctattccggt caacaagcta gctgatgcag aagtttttgc
     4021 aggaaccatt ttagaaaacg gaacgattca aatcagagct gaccgagttg gtgaggacac
     4081 cacatttgga aaaattattg aactcgtgga agaagcacaa gattctaaat ccgaagcgga
     4141 acggttcatt gatcgctttt ctaaatacta cacaccagct gtcttggttc tggcaattgt
     4201 tgtatgggcg ttcagtcaaa atattgagtt agcaatcacc atcttggttc taggttgccc
     4261 aggcgcatta gttatcggag tgcctgtctc aaacgttgcc ggtattggga atggtgctcg
     4321 taacggtgtt cttttaaaag ggagtgaagt cattcaagac ttcagtaatg tggatacaat
     4381 tgtatttgat aagacaggta ctttaactgt cggaaaccca accgttgcag cgactgaact
     4441 gtatgaaaaa gatcctgctg aaacgcttgg ctatctggcc agtgtggaac gggaatcaga
     4501 tcatccatta gcaaaagcgg tcttaaatca aattggagaa acaaactttt atcctgttga
     4561 aggaactgaa gttgtgaaag gcggcggaat cgtttcaagt gtagctggac atagagtggc
     4621 tgttgggaat gtggccttga tggaaaaaga aaatgtcact ctcagcaaaa aagtgcaaaa
     4681 agatgtcaaa cggtttgaac aacaagggaa ctctctcgtt ttgacagcgg tcgacggtga
     4741 attaaaagta ctgatgggca ttcgcgatca agtccgtccg ggtgtgaaag ctaatttgca
     4801 ggaattaaaa gacttgggcg tgaaaaatct agtcgttctt tcaggagata accaaggaac
     4861 cgttgatgtg gtcgccggcg aacttggttt gaccgaagct cacggctaca tgttgccgga
     4921 agacaaatcg gcttatatcg gaaaacttca acaacgtggt caaattgtag cctttgttgg
     4981 agatggcgtt aacgatagtc cgtccttagc tttagcagac attggaatcg caatgggaag
     5041 cggaacggac gtagcgattg aaacatccga tgtcgtttta atgaactcga acttcagcaa
     5101 cttgcctcac gcattaggat tagtaaaagc caccgcaaat aatatgaaac aaaatatcgt
     5161 gatttcagtt ggagtagtgt tggtcttgtt gaccagcgtc ttcttcagcg aatggatgaa
     5221 tatgtctatc ggaatgttgg ttcatgaagg tagtatcttg gtggtaattt tcaatggcat
     5281 gagattaatg aagtataagt tgaaaggtcg gaaagaacaa aaagaagtat cagcacctac
     5341 agagaaagta aaaatatctt aagaataaat tagaagaggc tgagacaata gtccatcctt
     5401 gaaatgacaa aagcggaaac tcagattttt aaatctggga ttttccgctt ttttgagttc
     5461 ttgaaaaaaa ataagagtat agagtatgtt ctttataaaa ttaattttcg tgtaaattat
     5521 taaaaattta ataataaaat ttaaattcct attaagttgt aacacatgaa attgcttaaa
     5581 atagtgtatc aaaaaccttg ttttattaat gttgaattta tttgaaaggt ctacagatta
     5641 acatattatt gacaaaacaa tatttaaata gttatacttc attcaaacgt ctgtttgaac
     5701 gaggtggtga agttaatgac tgcagaagta tgtacaacta cttgtataca taaagaaaaa
     5761 gtagctagtg ttaaaaataa tttaaaagat aaagaccttt caactttact tgtattagga
     5821 aaatgtttta gcgattcttc taggattaaa attttctatg ctttagaaac gtataaggag
     5881 atgtgcgttt gtgatttagc agcgatactt aatgctagta ttgctacaac gtcacaccat
     5941 ttacgttttt tgaaaaagaa cggaatggcg aaatcacgtc aatatggaaa ggtagtttat
     6001 tactctttag cgaatgaaga catcctttcc gctattcatg tttttttaaa gttatcagaa
     6061 aatctatcta tgaaatctta gtttggagga agttattttg ttacaaagta ttttttcagc
     6121 tatcgctgtt tatatttcta ccagcattga ttatttgttt atcttgctga ttattttttc
     6181 tcaaagtcat acaaaaaagg gctccgtcag attttttggg gtcaatatct aggaacaggt
     6241 atcttagtag ccgtaagttt atttgcagca tatgtgctga atttcattcc gcaagattgg
     6301 atcattgggc ttctcggtct aattccgatt ttcttaggaa tcagagtggc attggttggc
     6361 gaagaggagg aagaagaaga ggaagtcgtt gaaaagcttg aatcaagagg aacaaaccgc
     6421 ttcttttgga ccgttgcctt aataacaatt gcttctggtg gcgataactt agggatttat
     6481 attccttact ttgcctcgtt atctttttca gagattgtca ccgctctaat cgtatttgct
     6541 atttctgttg cggttctttg ctatatcagt tacaaattag ccaagatttc ttttgtttcc
     6601 gaaaccttag aaaaatatga acgaatcctt gttcctatag tgtttattgg cttaggaatt
     6661 tatattttga tagaaaatgg gacgattcaa acactattaa att
//
LOCUS       DAZUKC010000119         6659 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000119 DAZUKC010000000
VERSION     DAZUKC010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.122"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..468)
                     /locus_tag="ACG80T_002532"
     CDS             complement(<1..468)
                     /locus_tag="ACG80T_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate--amine ligase"
                     /protein_id="HFS4842144.1"
                     /translation="MNILILSCGRRCELVNAFKTALNNNEMEGKIIGADCSELAPALY
                     MCDEKIVLPKVSESNYIEELIKVIKFFEIKLIVPTIDTELSTLSRNKKYIENETDSVV
                     LVSSEKVIDICENKILTSAFFEQNGFKTPEILNSSEITKFPVFIKPLNGSSSVN"
     gene            complement(480..2297)
                     /locus_tag="ACG80T_002533"
     CDS             complement(480..2297)
                     /locus_tag="ACG80T_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFS4842145.1"
                     /translation="MFVLTRRRKIATLVVVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFSSLSASAASSILILLFINRRYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYVSADSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVVGFVDDDPNKRNTYLSGKKVLGALKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESARVKVNTMPSIEELASGKISVSKLKTIDVVDLLGRDEVKLDIESI
                     KDQITDKVILVTGAGGSIGSEICRQIIQFNPAKLLLLGHGENSIYLIDRELRTHHQNC
                     PTEIVPIIADIQDREKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPITVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSIQKVMDFVKSLPQDDEQLAKDIVTFANASNR"
     gene            complement(2310..3074)
                     /locus_tag="ACG80T_002534"
     CDS             complement(2310..3074)
                     /locus_tag="ACG80T_002534"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFS4842146.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNAHFRKNPNYLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIGKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(3102..3800)
                     /locus_tag="ACG80T_002535"
     CDS             complement(3102..3800)
                     /locus_tag="ACG80T_002535"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFS4842147.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLSNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQIFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLSKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(3812..4591)
                     /locus_tag="ACG80T_002536"
     CDS             complement(3812..4591)
                     /locus_tag="ACG80T_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFS4842148.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKEAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGSAPARPKATNGKTAPSRRSRSRV"
     gene            complement(4607..5551)
                     /locus_tag="ACG80T_002537"
     CDS             complement(4607..5551)
                     /locus_tag="ACG80T_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS4842149.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(5597..6376)
                     /locus_tag="ACG80T_002538"
     CDS             complement(5597..6376)
                     /locus_tag="ACG80T_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842150.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKNFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTIVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIDQIRNEKIRAAYATKAQTVAQQMGYSNISY"
BASE COUNT         1795 a         1358 c         1173 g         2333 t
ORIGIN      
        1 gttaacagat gaactaccat tcagaggttt tataaataca ggaaattttg taatttcaga
       61 agagtttaaa atttcaggag ttttaaaacc attttgttca aaaaaagcag aagttaaaat
      121 tttgttctca caaatgtcga tgactttttc tgaagaaact aatacaactg agtctgtttc
      181 attttcaata tattttttat ttcttgataa tgttgataat tcagtatcaa tagtaggaac
      241 aattaattta atttcaaaaa actttattac ttttattaat tcctcaatat aattactttc
      301 tgaaacttta ggtaatacta ttttttcgtc acacatatat agtgcgggtg ctagttcaga
      361 gcaatctgct ccaatgattt ttccttccat ttcattattg tttaatgctg ttttaaatgc
      421 attcactagt tcacaacgac gtccacaact taatattaaa atattcaatt tttaatctcc
      481 tatctattcg aagcatttgc aaaagtgaca atgtctttag ccagttgttc atcgtcttgt
      541 ggcaggctct taacaaaatc catcactttt tgtattgagt aacctttgat gtttcctaca
      601 aagattttct cataaactgc ttcatcattt ctttccttat ccaacaatag ttcttcatat
      661 agtttttccc ctggacggat acctgtttca atgatttcga tttcgtcttc gctatacccg
      721 ctcaatcgga tcatgttttt ggctaggtct acaatcttca ctggttcact catatctaga
      781 ataaaaattt cgccaccttt tgctaatgcg ccagattgaa taactaaacg actcgcttca
      841 ggaatcgtca tgaagtatcg ggtcatacga aagtctgtta cagtgattgg accaccttga
      901 gcaatttgtt ctctgaatac tggaatcaca ctaccgcgag aaccaaggac attcccaaat
      961 ctgacagctg agaatttcgt acagccttct tcattcaatc cagtcacgat catttcagcg
     1021 attcgttttg ttgaacccat gacgtttgga ggattgttag ctttatctgt cgataccatg
     1081 acgaagtttt tcacttttgc tgctttagct gcttctgcta cattttttgt accgaaaata
     1141 ttgtttttga ctgcttcttt tggattatat tccatcagtg gtacatgctt atgagcagct
     1201 gcatggtaga cgatatctgg atgatattgt tccatgatct cattgatttt ctcgcgatct
     1261 tggatatctg cgatgatcgg tacgatttct gtaggacaat tttgatgatg cgtgcgtaac
     1321 tcacggtcaa tcaagtagat cgagttctcc ccatgtccta aaagcaatag ctttgcagga
     1381 ttgaattgaa tgatttgccg acagatttct gaaccgatcg atcctcctgc gccggtgaca
     1441 agaatgactt tgtctgtgat ctgatctttg atggattcaa tatccagctt tacctcatct
     1501 cttcccagta aatccactac gtcaattgtt tttaattttg atacgctaat ttttcctgaa
     1561 gcaagttctt ctatagaagg catcgtgttg actttcacac gagcggattc gactaactcg
     1621 aagattcttc gtaatttttt tcttgataag gaagggatag cgatcgttac catttgaatg
     1681 tcatattttt caatcaattc cggaatatct tttaatgcac ctaatacttt ttttcctgaa
     1741 agatacgtat ttcttttatt tggatcatca tctacgaacc cgacaacgtg gatatcttga
     1801 gccgttttgg atccaagaaa gctgttgtat aaaatacgcc cgccttcgcc agcaccaaca
     1861 atcaatgtat ttttagcaga atctgctgat acatatctca tattttttgt ttcgatgtag
     1921 atacgccaga tcaatcggct accgatgatc aataagagcg aaagcagata agcaaagata
     1981 actaaccgta aactgtatcg ccgattaatg aacagcagta tgaggatact tgaggcagca
     2041 cttgcagata gtgaactaaa aattgcaatg atctctctta aatttgtata gcgattgatt
     2101 cgagtgaaaa ctttaaataa actaccataa aataaataaa aaccaatcga caaaccaata
     2161 gagattaaaa taaggtccat tggtatagct acaaacggct tcatgaattt agctgctgca
     2221 atatttgcag cgattaataa caagctgtcc acaacaacca atgtggcaat ttttcttctt
     2281 cttgttaata caaacaccct acattccccc taaaataaac caaatttctt cttttttacc
     2341 tcttcataga ctggataact gatttcatcc ccgttgatca gatcctttgc gacttgtttc
     2401 atctgtacta ctttttcctt accaaagtcc ttgccaatct gctcatagca ctcttttaag
     2461 taaaaggtgc gcttagagac cccatgggca tcagaagcag ccatctgcac gagattatgt
     2521 ttgaccatcg ttttagctgt tttctggatg tctttgccaa acaccccaac ataacttggc
     2581 gcagtaagtt gggccaaaca ccccatatct aggaacggaa gcaaataatt cgggttttta
     2641 cgaaaatgcg cgttacgttc tggatggacg atcactggtg tctttcctag ttctaacaac
     2701 tcaaaaaaaa gccgttctgc atataacggc acttccagtg tcggaaattc gatcagaaga
     2761 tatgtatcat ctaaatctgt aaataaaatt tcatctcggt ggatgtcttc gatgagcgtt
     2821 ccggtaatac gtacttcttg tccttccaat aacgtcaaag gcaattgtct tttctctaat
     2881 tcggcttgta acgaagcgac tagagaaatg acttgtgatt tttcattgct gtattttcca
     2941 ttattgtggt gtggtgtgca taaaatatgc gtgatgcctt gctgaatcgc tttttcagcc
     3001 attgcaatac ttgcttctaa gttttctgcc ccgtcatcga ttcctggtag gatatggcaa
     3061 tgtagatcga tcatttctct tgtctcctct cttcttattc cttaatttcc gtagtaataa
     3121 ccagcatctt tgctatgttc tgctccgttg tatacaacac ctaagactcg tgcttgtacc
     3181 atctctagca gttcttttgc tttagacaag gattctttac gtgatgtatt ttcacgtacg
     3241 accaataaag tgccatccgt ttttgaagac atgatctgtg catctgttac ggctactact
     3301 ggtggcatat cgaatatcac cacgtcaaat atctgtcttg cttcggctag aatctgatcc
     3361 attcttggcg agcttaacaa ttctgatggg ttgggtggct taggcccact aggcaaaatc
     3421 gacaagttct cgactggcgt tcgttggatc acatctgcta cacttcctga tgaactaagt
     3481 gctgtactta atccactagc attgctcaac tggaatgttt tatagacgac gggtttacgt
     3541 aaatctgcat cgactaatag aacccgttga ccggatttag caaaaacgac tgcaatgttt
     3601 gctgcagtgg tggattttcc ttctccagga cctgatgaag tcacgacaat cgttttgatt
     3661 tgctgaccag ctgcggaggc aaactggata ttcgtccgga tcgtacgata ttgttcggcg
     3721 attggtgaag atgggtttgt taatgtgatc aaactgactg catgagtttg tgctgctttt
     3781 tgtttctgtg ttcgtgccat ttttcttcct cctagactct tgagcggctt ctgcgtgatg
     3841 gcgctgtttt accatttgta gcttttggtc gtgcaggagc tgatcctttt ttacggatac
     3901 cagcgttcaa ttctttcgct gacatttctg gaactgttcc taagattggg aatcctaatg
     3961 tatccgtcac atatttttca tctttcactg tccgatcgag taattcgaat aagaatgcca
     4021 gacctacgcc taccatcatt cctaatacta agccaatcac taaattcaat ttattatttg
     4081 gcgaaacagg tgtcgtattg gcaactgcac ctgaaataat cgagatttta tcgacattca
     4141 tcacgtcttt tgcgttctct tggaaaactt gtgaagttgt gttagcgatc tgttgagcag
     4201 tcactgcatt cgtgcttatt gcctggatcg agaacatttg tgaattttgt gactgattga
     4261 ctgagatggc ctctttgatt tgttcagcag tcattttcac attgtcttct gtttctaaac
     4321 gatctttgac ttcatttagc actaaatcac tgacgatcat atctttatac gtattgatca
     4381 tttgcaggtt cgtattgaca tcattgacat ttgctgtgtc tgattgtggc agtgtcacga
     4441 tcaattgtgc ttgtgaactg tattttggtg tcaagatgaa aaatgttacg attcctgaga
     4501 tggccaagcc agcaaacatt gagatgatga ttgttaccaa gtgtttgcgt aatgtttcaa
     4561 aaatttcttt caagctgatt gtctcttcca ttttttctcc actttcctat ttattctcta
     4621 gattcatttg atcttttaat tcttttttca cacgatcaag ttcttgatcg ctgacttttt
     4681 gataggagat attatcttgc atgaagcctt ctccttgcag ctgatcttgc ttgatcgtac
     4741 caaatgcctc ccgataatct aaagcaattt ttttcatttc atcaaagctc atgtctgttt
     4801 tcatattcga ctctagagca ttcaaaacgg attgatagtt gctgacaccg tttagactca
     4861 atactttttt cgctacacct tctacgatct ttctttgacg ctcttgtcta ccatagtcac
     4921 catttggatc ttcataacgc atacgagaat aagataatgc ctgatccccg ttcaaagtaa
     4981 ttttaccgat tgtaaagtca tagccatctt gtgagaaagt gaggtcatta ttgacttcga
     5041 taccaccaac tgcatcaaca agttctttta gccctttcat gttgatagag acatagtgat
     5101 cgattgggat gtctacatat ttttgtacgg tatccatcgc catggcagct ccgccgaatg
     5161 cgtaagcgtg gttgatcttg tcttttgtgt cacgacctac gatgtcgaca tacgtatctc
     5221 gaggaatgct gacgatcgtt gtctgtttgt cttcaggact gactgtggca accatcatcg
     5281 tatctgatct tccttgatcg acccgaccta aatctccagt atcaatgccc atcaatagaa
     5341 cagagaaaga ttcttgtttg tccagatcaa ctggtttcgt tcgttctcct tgggttcgtt
     5401 ccactgattc gtaagttttc tcaatagaat gtgagagatc cagatataat tttgctccta
     5461 ctcctacaat tgctaatact aatacgataa gaactgctaa aataatcaga aatatttttt
     5521 tccctcttga ttttcttctt cgtcttgcca tagtttcctc cgtttgctaa ccttgcttga
     5581 atcaacgttt taactgtcaa tagcttatat tgctatagcc catttgctga gcaacagttt
     5641 gtgcttttgt tgcgtatgct gcgcgtattt tttcattccg gatctgatct atttgactga
     5701 gcaccgtcaa atattgatcg taagtagcgg tggcagtcac ttgaccatcc ttcaacatcg
     5761 tatcgaataa ctgatctagt tgcgtgactt gcgcgatctg agtatcagcg aatcctagat
     5821 attgcataac aatcgttttc caaggactat ctttaaaaaa gctcatattt tcacgtacac
     5881 gagcgacatc tgcacttgtc accttttctt tgatgacgac atcgtctaca gcttgctgcc
     5941 agtttgatac atttttagta aataaatcgt tgacagctgt ctggatcttc agttttgatt
     6001 ctgcactatc taactgtgtc acgattgctt tcttctcatc ttggattgca acagcttctt
     6061 taggcaattc tgattctttg atgtcgtaat catctgctga aagtttgaca gaggatagct
     6121 tatttgacaa acttgtgatt gtttcttctg ttatttcagg gtttagaaaa tttttttctt
     6181 ttgtactata cagcttgtct acttcttttg ataaagaagt caaggtgtct ttttgattgg
     6241 tgatggcaga ttctgcataa ttgatccgtt gctgatgttg ccgctgatta ttgatatggt
     6301 aaccaaacaa acagatcgca atagcacctg cacctaccgc cacagcagtc aatgacttga
     6361 cgaatggctt ttccatagat tcactcctaa gtattgatga tagataaatt aagatataat
     6421 tcacttacat aaaaatgaca gctttttatt ttaaaaaaca taattaaact aatatattca
     6481 cagaaatatt tgtaaacaag ttttccctat ataatcatac cacaaactgt tttcttgact
     6541 agggcaattt acgatttaaa aataaataaa acattaataa gataacaatt aatcgatttc
     6601 atttttattt ccttaatttt cataggtgtt tttaaaagtt acacaaaaaa taaaataac
//
LOCUS       DAZUKC010000120         6644 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000120 DAZUKC010000000
VERSION     DAZUKC010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.125"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..859)
                     /locus_tag="ACG80T_002539"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ACG80T_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG80T_002540"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG80T_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFS4842151.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ACG80T_002541"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ACG80T_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="ACG80T_002542"
     CDS             complement(2143..2373)
                     /locus_tag="ACG80T_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFS4842152.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="ACG80T_002543"
     CDS             complement(2603..3421)
                     /locus_tag="ACG80T_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4842153.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="ACG80T_002544"
     CDS             3582..4271
                     /locus_tag="ACG80T_002544"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFS4842154.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="ACG80T_002545"
     CDS             4285..5787
                     /locus_tag="ACG80T_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4842155.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="ACG80T_002546"
     CDS             5800..6276
                     /locus_tag="ACG80T_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFS4842156.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="ACG80T_002547"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="ACG80T_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1204 g         2236 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DAZUKC010000121         6535 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000121 DAZUKC010000000
VERSION     DAZUKC010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.126"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            89..943
                     /locus_tag="ACG80T_002548"
     CDS             89..943
                     /locus_tag="ACG80T_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFS4842157.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            944..2281
                     /gene="xseA"
                     /locus_tag="ACG80T_002549"
     CDS             944..2281
                     /gene="xseA"
                     /locus_tag="ACG80T_002549"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFS4842158.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            2286..2510
                     /locus_tag="ACG80T_002550"
     CDS             2286..2510
                     /locus_tag="ACG80T_002550"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFS4842159.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            2512..3402
                     /locus_tag="ACG80T_002551"
     CDS             2512..3402
                     /locus_tag="ACG80T_002551"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFS4842160.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            3411..4229
                     /locus_tag="ACG80T_002552"
     CDS             3411..4229
                     /locus_tag="ACG80T_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFS4842161.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            4336..4785
                     /locus_tag="ACG80T_002553"
     CDS             4336..4785
                     /locus_tag="ACG80T_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFS4842162.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            4803..6485
                     /gene="recN"
                     /locus_tag="ACG80T_002554"
     CDS             4803..6485
                     /gene="recN"
                     /locus_tag="ACG80T_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFS4842163.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
BASE COUNT         2208 a         1102 c         1506 g         1719 t
ORIGIN      
        1 ttttttctat gaatcatcat aaacctgtta taaatcggga aaattctatg gtagaatgaa
       61 aacacaaaaa atatctggga gtggttagat ggcagaattg attaacggaa aagaattagc
      121 tgaaaaaatg caggcagaga ttgctgaaaa aatccaaaaa ttgaaagata atggtatcca
      181 tcccggatta gtcgttttat tggtagggga aaatccagcc agccaaatct atgtaagaaa
      241 taaagaacgt gtagcaaaag aaatcggaat ccattcattg gtagagcgtt atcctgagac
      301 gatttctgaa gacgaactac ttgcagaaat tgaaaaatac aatcaagatc ctagttttca
      361 tggtatattg gtccaattac cattgccaaa gcatatagat gaagaaaaga tcctgttagc
      421 tatccgccct gaaaaagacg tagacggttt tcatccgatg aatttaggca gactccttgc
      481 gggtaaaccg gacaagattc cttgtactcc atacggcatc atgaaaatgt ttgaagctta
      541 ttctatcgat ccggcgggta aacgagcagt agttatcgga cgaagcaata tcgtcggtaa
      601 accgatggct cagctactct tgatggcaga tgcaactgtt actgttgctc attctaaaac
      661 cgaaaatctg gctgaactgg cacaagaagc agatatttta gtcgtagcaa taggacgtgg
      721 acattttgtc acaaaagaat tcgtaaaacc aggtgctgta gtgatagatg tagggatgaa
      781 tcgtgatgct aacggtaagc tgatcggtga tgtgaaattt gatgaagtgg agcctattgc
      841 aggctggatc actcctgtgc caaagggtgt agggccaatg actatcacga tgctgatgta
      901 tcaaaccgta aaatctgccg aagcttttgg agcaggtgaa tagatggcac aagaatattt
      961 aactgtcact acattaacga agtatttgaa aagaaaattt gatgccgatc cttatttaga
     1021 acgcgtatat ttaacaggcg aaatatcgaa tttccgttta cgtgccaatg cccatcaata
     1081 tttcagctta aaagatgacc atgcaaaaat atctgctatt atgttcaaag gagcattcca
     1141 aaaattacga ttccagccaa aagaaggaat gaaagtcctt gtcgttgggc gtatttcatt
     1201 atatgaagcg ggaggttctt atcaaatcta tgtagagcac atggagcctg atggaatagg
     1261 tgctttatat caagaattgg aagaacgaaa agaaaagctg atgaaggaag gcctttttca
     1321 agggcctaaa aaaatgctgc cacgtttccc aaagcgaatc gcggtcctta ccagtccaag
     1381 cggagcggtt atccgcgata tcatcacgac ggtgaaaaga agatatccaa ttgtccaatt
     1441 ggttttgttc ccaacagttg tacaaggaaa ccaagcagcg gacaatgttg tcaggaatat
     1501 ccagagagta gaagagatgg gcaatttcga tacaatgatc atcggtcgtg ggggtggttc
     1561 gattgaggat ttatggccgt ttaatgaaga gcgggttgct cgggcgattt atcaagctaa
     1621 tacgccggtg atttcatctg taggacatga aacagatacc acgatcgctg atttagtagc
     1681 agatgttcgg gctgccactc caacagcagc tgcagaatta gctgttcctg ttttgtcaga
     1741 agagttgatg cgcattcaag aacggcaagc tagattgcag caggcgtttg tacgccagat
     1801 ccagcgcaaa caagaacggt tcgagcgtgc tcgacgttcc tatgtatttc ggcagcctga
     1861 aagattgtat gaaggtcaga ccatcaaatt agaccagttg aaacagcgat tatatcaagc
     1921 tacacagcaa atttatcatg cgaaagaaaa acaaacgatc gcattaagcc accaattgga
     1981 acaagtggct ccgctttatc gagttaaagc agctcgccaa gaaactgctt atctagaaaa
     2041 acgcttagaa gagaaaatcc aacaatatat gaaacaacag cagcagcgtt ttgctcaagc
     2101 tgttcaatct cttgatttgt tgagtccatt gaaaatcatg ggaagaggct acagctatac
     2161 gacgttaaat gatcaagtag taaaaagtgt caccgagatt catcaagggg atttgctgac
     2221 gatccattat caagatggaa tcgttaaagg acaagtacaa gagatagaca aaggagaata
     2281 aaaatatgga aaatccaact tttgaagagt cactgcaaga attagaaaaa attgtcatgc
     2341 agttagaaca aggagacgtt ccattagaat cagctctaga ttcgtttaaa cgaggaatgg
     2401 aattaagcaa acattgccaa gatacattag ctaaagcaga agagaccttg acgaaaatga
     2461 tgacagaatc aaatgaagaa gttgattttg acggaaacga ggaaagctga gatgagatta
     2521 actgactttt cagctattca tctacctgct gtagaaaaag aaatcttgtc ttttttagat
     2581 gagcatacaa cggatagagg actctatgat gcgatgagtt attcagtgaa agcaggcggg
     2641 aaaagaatac gtcccttatt acttttaacg gccgttgctt cctttgatga gcctattgat
     2701 gttcccgtct atcaagtagc agctgcttta gaaatggtgc atacgtattc cttgatccac
     2761 gatgatttgc ctgcgatgga caatgatgat cttcgtagag gaaaacctac caatcataaa
     2821 gtattcggcg aagcattagc tatattggcc ggagatggtt tgcttacagg agcattccag
     2881 ctaatcagta tggcacactt aggtaattct cctaagcttc tgctgcttca gcaattagct
     2941 gtttgtgcgg gatcacaagg tatggttgct gggcaagctg cagatatcga gggcgaatcg
     3001 aaaaagcttt ctttagaaga attagcattt attcatgaaa gaaagacagg gcatttgatt
     3061 cgttatgcct tgctggctgg tggcatatta gcgaagcaac ctgaagaaat cttattgcta
     3121 cttcaacgat tagccgaaca tttaggttta gcttttcaga taagagatga tctactcgat
     3181 gtgattggta caacgaaaac attagggaaa acagctggta aagatgaacg tatggaaaaa
     3241 aatacttacc cgcgtttatt aggcctagaa aagacacgtg aggcgttgga gattgagctg
     3301 ttatccgcca acaagatcat agacaaatta gaagaggacg ttttttcttt tgatggcggg
     3361 ctgatgcgcc agatgatcaa gcaatttgat gtggagaaat gagggaaaga atggaaaaag
     3421 aacgcgtaga tgtattagcg gtcaaacaag gattatttga aacaagagaa caagccaaac
     3481 gttcagtgat ggctggtttg atttacaacg aaaaaaacga gcggtttgat aaaccgggag
     3541 aaaaaattcc agtgagtagt gaattaagag tcaaaggtaa aaaacttcct tatgtttctc
     3601 gagggggatt aaaattagaa aaagcactca aagcatttga tctatctgtt gaaggaaaaa
     3661 cattgttaga tattggtgca tccactggcg gatttactga tgcagctttg caaaatggcg
     3721 caaaaatgag ttatgcttta gacgttggct acaatcagct agcttggaaa ataagacaag
     3781 atcctagagt cgtcgtaatg gaaagagtga attttcgtta tgcaaaaccc gaagacttta
     3841 ccttaggaat tccagaagtt gctgttatcg atgtttcttt tatttcacta aaactgatgc
     3901 tgcctccatt acatgctatt ttaaaagaag agggagaagt gattgcttta atcaaacccc
     3961 aattcgaagc aggcagagaa gctgtaggga aaaacggaat tgttcgtgac cctcagaccc
     4021 atcaaaaagt tttagaagac attttgtcat ttgcggcaca aggtggatat gatgtgctgg
     4081 aattaagtta ttctccgatc acggggggag aaggaaatat cgagttcttg gctcacttga
     4141 ggaaagtacc cgaaagcgga acaatcaatt cagctattaa tatggcagaa gtggtttcaa
     4201 atgcacacga acagtttgac cataaataaa ttgtaactgt gaaaatcaag tgagaagtat
     4261 gcggattctt ctttattata ccaaggaaat aagctatcat tgattaagaa gaatatgcta
     4321 aggagcgaag acgtaatgag aaaaaaagat cggcatcgtt tgattactcg attgttgagt
     4381 gagcaagata tccgaaagca ggaagaattt gttgaaatcc tgaaaagcaa aggaatatct
     4441 gttactcaag ctacgatttc cagagatata aaggaactga aactgatcaa ggtaccggct
     4501 tcagatggcg gctaccgtta tagcatacca gctgaaacaa acgaagatgt aaatactaaa
     4561 ttggaaaaac tattaaaaga cgctttcgtg gcagttgacc aaatggaaaa atttgttatt
     4621 ttgaaaacat tgccgggaaa tgcttcggca gcagcaaatt tgattgataa acgttacaag
     4681 aaagaattat tttcgattat caatgatgac gataatgttt tgatgattac acgaactgaa
     4741 gaccaggcag ttttattgaa gaaagacttc ctgcattacc tttaagttga ggagcgaatg
     4801 tgatgctaca agaaatcagt attactaact ttgcgattat tccggaattg cggctttctt
     4861 ttcacgaagg aatgaccgcg ctgaccggag agactggtgc gggtaaatcc attattatcg
     4921 atgctttagg tctgttagca ggaggaagag gttcaagcga ttatatacgt caaggagcag
     4981 aaaaatgtgt gcttgaaggg ttgtttgaac taccaaaaca agaaggattc tctgaactga
     5041 tggttgaatt agggatcgaa acagatgagg acaatctgat tgttcgacgg gatatgtcct
     5101 taactggaaa aaatgtttgt cgagtgaatg gtcatattat cacattagct aatctgagaa
     5161 gaatcggcag ttatttagta gatatccaag gacaaaacga acatcaggaa ctgctccagc
     5221 ctgaatcaca tcttgcttta ttggatcgtt ttggagatgt agctttccaa cagaagaaaa
     5281 agagttatca gcaagaatat atctcttatc gtgagctgga aaaaagggta cgaaaagtgc
     5341 agcaaaatga aaaatcctat gttcagagaa tagatatgct ccatttccag caagaagaaa
     5401 tagctgctgc tgatttgcaa gtcggagaag aagagaaatt aaaagaagaa agagaaaaac
     5461 ttagcaatta tcaaaaaatt gctgatggat tagctgcagg ttacggagca ctgacgaata
     5521 atgaacaaaa cagtgtagat ggtgttggct tggccgtttc agaactgcaa gggatcgccc
     5581 atctagatgt ggagtatgaa gcaatttatg aaaatatcca aagtgcttac tatttgctgc
     5641 aagatgcaat cggcgatatg agtcgtcaga ttgatttatt ggagttagat gagagtcgtt
     5701 tagaagaagt tacgcaacgt ttagaattaa tccgacagtt gaaacgtaaa tacggcgaat
     5761 ccattgagtc aatattggct tactatgacg aaatcaccga agagctagct tcttctgatt
     5821 tttcagaagg tcaattggac aaaatgaaag aggaactaga gcaaaaagag cttctacttc
     5881 agcaacaagc cgcagatctt catgaagcgc gtaaaaaaat cgcaaaagaa ttggaaaaat
     5941 ccattctgca tgaattgaaa agtctgtata tggaaaacac tgagtttgag gtacgtttct
     6001 taaaagaaga aaatagacaa ttgaatcatg atggatttga ccaaatcgaa ttttacatta
     6061 cgaccaatcc aggagagcca ctgaagcctt tggtgaaagt cgcttctggt ggcgaactgt
     6121 ctcggatgtt gcttgctttg aaaactattt tttcttctga gcaaggtgtg acaagtatta
     6181 tttttgatga agtggataca ggtgtgagcg gaagagttgc acaggccatc gcagataaaa
     6241 tttctcaaat atcgaaatac tctcaagttt tgtgcatcac tcatctgcct caagttgctg
     6301 cagtcgccga ctatcaatat tatattgtga aagcagtgat tgatggacgt acgcaaactt
     6361 ctgtttcaga gttgaaaaca aaagaaagag aagaagaaat tgcacgtatg ctggctggaa
     6421 gtgagattac aaaactcaca ctcgaacatg caaaagaact attgaaactc gcaaaaagga
     6481 cctgagccac ctcgcagtgg ttcaggtcct tttcagcagc tgatagctat atttt
//
LOCUS       DAZUKC010000122         6394 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000122 DAZUKC010000000
VERSION     DAZUKC010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.127"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACG80T_002555"
     CDS             complement(99..1229)
                     /locus_tag="ACG80T_002555"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFS4842164.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ACG80T_002556"
     CDS             complement(1878..3080)
                     /locus_tag="ACG80T_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4842165.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ACG80T_002557"
     CDS             complement(3140..3343)
                     /locus_tag="ACG80T_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFS4842166.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4552)
                     /locus_tag="ACG80T_002558"
     CDS             complement(3485..4552)
                     /locus_tag="ACG80T_002558"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS4842167.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(4610..4717)
                     /locus_tag="ACG80T_002559"
     CDS             complement(4610..4717)
                     /locus_tag="ACG80T_002559"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4842168.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4880..5692
                     /locus_tag="ACG80T_002560"
     CDS             4880..5692
                     /locus_tag="ACG80T_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4842169.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6066..6257)
                     /locus_tag="ACG80T_002561"
     CDS             complement(6066..6257)
                     /locus_tag="ACG80T_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842170.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT         1916 a         1307 c         1006 g         2165 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttct
//
LOCUS       DAZUKC010000123         6173 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000123 DAZUKC010000000
VERSION     DAZUKC010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.128"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            725..811
                     /locus_tag="ACG80T_002562"
     CDS             725..811
                     /locus_tag="ACG80T_002562"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFS4842171.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="ACG80T_002563"
     CDS             complement(932..1621)
                     /locus_tag="ACG80T_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842172.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1866..2780)
                     /locus_tag="ACG80T_002564"
     CDS             complement(1866..2780)
                     /locus_tag="ACG80T_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFS4842173.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="ACG80T_002565"
     CDS             complement(2762..3142)
                     /locus_tag="ACG80T_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFS4842174.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="ACG80T_002566"
     CDS             complement(3592..3879)
                     /locus_tag="ACG80T_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFS4842175.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="ACG80T_002567"
     CDS             complement(3899..4123)
                     /locus_tag="ACG80T_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFS4842176.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="ACG80T_002568"
     CDS             complement(4578..4748)
                     /locus_tag="ACG80T_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842177.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="ACG80T_002569"
     CDS             complement(4763..5296)
                     /locus_tag="ACG80T_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFS4842178.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..>6173)
                     /locus_tag="ACG80T_002570"
     CDS             complement(5317..>6173)
                     /locus_tag="ACG80T_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFS4842179.1"
                     /translation="IVSRMREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEK
                     ILSLRFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYS
                     LQQFNELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPI
                     RQELTAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALAL
                     ADKQKEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLL
                     SDLDNLFQ"
BASE COUNT         1632 a         1151 c         1042 g         2348 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga atgactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DAZUKC010000124         6161 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000124 DAZUKC010000000
VERSION     DAZUKC010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.129"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..355
                     /locus_tag="ACG80T_002571"
     CDS             <1..355
                     /locus_tag="ACG80T_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842180.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
     gene            615..1076
                     /locus_tag="ACG80T_002572"
     CDS             615..1076
                     /locus_tag="ACG80T_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermophilic serine proteinase"
                     /protein_id="HFS4842181.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRTNESDYNKRDQVLL
                     VKLNEEISVEEAVRTFNASDMVNYAEPNYVVELEPLNDVEEPNTTDSLGNR"
     gene            1120..1206
                     /locus_tag="ACG80T_002573"
                     /pseudo
     CDS             1120..1206
                     /locus_tag="ACG80T_002573"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            complement(1172..2242)
                     /locus_tag="ACG80T_002574"
     CDS             complement(1172..2242)
                     /locus_tag="ACG80T_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4842182.1"
                     /translation="MKNDIVTYANTKCEEIGISNLEECFFDTNDLSQFLRTGRTNASK
                     LANDLVAKNVLFKINTRPVLFAIKERFEEHFLIPVKDVYESISELKTDLEPNSTDSIL
                     QKMIGYDMSLSDAVEQMKTAVFYPGRGLPMMITGDTGVGKSFFVHTAYQFMKKVNVIH
                     ENAPFKVLNCAQYHNNPELLSGILFGYVKGAFTGADEDRNGLLQDADGGVLFLDEVHR
                     LPPEGQEKLFVYMDGGTYSRVGETIHHASNVRLVFATTEGKEDFLETFMRRIPIHIHI
                     PNIEERGILEKNKLSKILSLAKAKTLARLLMFLLKSLVLHKYSQNILNYYFLEKSKKN
                     RIPNFHLHFQKDLHVDNQFLNQ"
     gene            2844..4688
                     /locus_tag="ACG80T_002575"
     CDS             2844..4688
                     /locus_tag="ACG80T_002575"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS4842183.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSNTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
     gene            4744..>6161
                     /locus_tag="ACG80T_002576"
     CDS             4744..>6161
                     /locus_tag="ACG80T_002576"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016620473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4842184.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSF"
BASE COUNT         2044 a          985 c         1247 g         1885 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taaacaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcgt
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aataagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
      601 aggaggagat gtagatgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
      661 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
      721 aaaggattat gagccaatta gatgactctc aaatagagta tgtacccaat gagctaattg
      781 tcagtttaaa ggctacacaa agaatttcaa cagagaaaaa taaaccgcaa aatttattgg
      841 gtgtaaaagt tatggtggaa aaagatttga ctcttaggac aaatgaaagc gattataata
      901 agcgagatca agtattatta gtgaaattga acgaagaaat tagtgtggaa gaggcagtaa
      961 gaacatttaa tgcttctgat atggtcaatt atgcagagcc aaactatgtg gttgaactag
     1021 agccgttgaa tgatgttgag gagccaaata caactgattc tttaggaaat cgataacgat
     1081 cctaattgga gtaaacaagc taatttaaga gagattagca tgccatccgc ttgggaagaa
     1141 accaagggtt ctaaggatgt gaaggtagcg gtcattgatt caggaattga ttatcaacat
     1201 gaagatcttt ctggaaatgt agatgaaagt ttgggatacg atttttttta cttttttcaa
     1261 gaaaataata attcagaata ttctgactat atttatgcaa cactagtgac tttaggagaa
     1321 acatcaatag ccttgctaaa gtttttgctt tcgctaaaga gagtattttc gataatttgt
     1381 ttttttctaa aatacctcgt tcctcaatat ttgggatatg gatatgtata gggattcttc
     1441 gcataaaggt ttccagaaaa tcctctttgc cttctgtagt cgcaaaaact aaacgtacat
     1501 tggaggcgtg atggatagtt tcaccgactc tagaataagt tccgccatcc atataaacaa
     1561 atagtttctc ttgcccttca ggaggcaatc tgtgtacttc gtctaaaaac agtactccac
     1621 cgtctgcatc ttgcaacaat ccatttcgat cttcatctgc gccggtaaaa gcacctttaa
     1681 catagccaaa tagtatcccc gacaataatt caggattatt atggtattgc gcacaattaa
     1741 gtactttaaa aggagcattc tcatgaataa cgttcacttt tttcataaac tgataagcag
     1801 tatgaacaaa aaaacttttt ccaactccag tatctccagt gatcatcata ggaagccctc
     1861 ttccaggata gaagacagca gttttcattt gttccaccgc atcggataag ctcatatcat
     1921 acccaatcat cttttgtaga atggaatctg tagaattggg ctccaagtct gtttttaatt
     1981 cagatatact ttcgtataca tcttttaccg gaatcaaaaa gtgctcttca aagcgctctt
     2041 tgatggcaaa taacactgga cgagtattga ttttaaatag aacgttcttt gcaactaagt
     2101 cattggcaag tttactggca tttgttcttc ccgttcttaa aaattgcgac aggtcatttg
     2161 tgtcaaaaaa gcactcttct aaattactga tgccaatttc ttcacatttt gtgtttgcat
     2221 aagtcactat atcatttttc atcttataaa cagtcctttc taaacttcta tcccttaaat
     2281 aaatagcaaa aaaaaggaag caatatcgat atgctgtcct cttaatttaa taaagcgcta
     2341 atctaattca ctgtattatc attttttagt agtatagcac aattatgaat gccttttctt
     2401 tatatcaaat aaagtttata taaaaaacct catcctgcaa gaaagaaaaa atgaacccat
     2461 tctttttata ctaggtctta aaaagacaga acatctgcta gaagatagaa gagaataaga
     2521 gttatatatg ttggaagata ttcttagaaa aaagacaaaa cttttagaac acgaaaacta
     2581 tattgaacga ataaaatgaa aaaatgatta aaaaatcatt ttattattta cgtttacatt
     2641 ctaatatgtt aaattgaata taacttaaac aggcatgtga ctggtaatgc aggcaggacc
     2701 taaataagtt gaataacaat gtatgcgttt caatacacgt ttgccttgtt gtcaatttat
     2761 ttagtttctg cctgtttttt gtgttgagga tggccacttc gactcaaaag ttcatcactc
     2821 aaaaaagaaa agagggaaac accatgaatt acaaacaaac agcagaagaa gtattgcagt
     2881 acatcggtgg ggaagaaaac atatcccatt tggagcattg ctcaactcgt ttacgtttta
     2941 cgttaaaaga caacagcaag gtaaatatga gccagttaga aaaagtggat ggcgttatgg
     3001 gcgttcgcca aaatgtgcaa tgccaagtaa tcattggaaa cgatgttgtt gaagtgtatg
     3061 atgaagtgaa agcgttatta ggtgacaatg tttcaaatac tgaagaagca ccaaaacaaa
     3121 aacaaaaatt aggttcagtt ctattagatt ttattatttc tattttccaa cctttaattc
     3181 ctgcgattgc aggtggagga gtattgaagt ccttgttgct actagcttct atcttagggc
     3241 tgattagtga ccaatctcaa acctatcaga ttttaaattt ggtcggcggg gcaccgcttt
     3301 atttcttgcc tattctggtg gcaatgacta cggctaataa attgaaagtc aatcaattgg
     3361 ttgcagtatc tgctgtcggt gcattgatcc ttcctgaaat gacagctatg ttggcggaag
     3421 gtgctacatt cttatctttt ggattggata atatagcgta tgcatctcaa gtattcccag
     3481 ccatcttaac tgttttattt tatgctcaac ttgaaaaatg gtttacaaaa gtttcaccaa
     3541 aaccaattcg tattttcttt gtacctatga tgagcttatt gattacagtt cctattgctt
     3601 tgttgcttct aggaccatta ggttataatg taggaacagc tttctcttct atcattgtgt
     3661 ctttgtacaa tcaatttgga tgggttgcaa ccggagtctt agcagctgta ctgccattaa
     3721 tggttgtaac gggtatgcat aaagcaatga tcccttacgc tgtttcatcc atgagtgaat
     3781 tgggtgcaga acttctttat ctcccggctt cattggcaca caatatttca gaagctggcg
     3841 catgctttgc tgtcagtatc aaaacaaaag acaaaaaatt gaaatctacg gctgtttctg
     3901 cgggtatatc tgcattattc ggaattactg aaccagctct ttacggggta acgattctgc
     3961 acaaacgtgt tttatatgcc gttatgtttt ctagcctgat cgggggagca tttgcaggaa
     4021 tcgttgcact tcaagccttt gctttagtgg gtcctgggat tgcaagtatc acgatgttcg
     4081 ctgacaaagc tgacccaatg aatctggttt gggcatttgc agtatcgggg atttcatttg
     4141 tggttgcttt tgctgcagcg ttggttcttt ataaagataa agaagttgtc aatgaagttg
     4201 aaactaagat ggaaacggcc gttgtttcag atgctgttct ttcaagtccc gtgaatggaa
     4261 aagtaatctc actatcagaa gtaaaagaca ctgttttttc tagtggtatg gtaggtgatg
     4321 gagtagcaat cattcctgaa gaagggatat tagtagctcc tgaagatgga gaaatcacaa
     4381 tggtatttga aacgaagcat gctattggtt taaaaacggc taatggtgca gaacttcttt
     4441 ttcatattgg tattgatact gttcagttag aaggaaaagg gtttgaggca tttgttcgtt
     4501 ctggtgaaaa agtagccaaa ggacaaaagc tgattgcatt tgatattgat ttaatcaaag
     4561 aagcaggatt cgaccccaca gttatctgtg tggtaaccaa taaggataat tacactatcc
     4621 gttcatttaa cctagaaaaa ttggtaacca atgatagtga tctcttagct atcagtccga
     4681 tagcttaaac acaagatagt gaaaaaaaat atattgaatg aaggaataga ggagaataga
     4741 ataatgacaa acacaacaag ttttccaaaa ggatttttat ggggcggcgc gatagcagca
     4801 aatcaagtag aaggtgcttg gaatgtagac ggtaaaggtc tttcagtagc ggatatggct
     4861 acttataaac caaatttaga caacaaagat tttgctgctc atatgcatgt caccctgcag
     4921 tctataaaag aagctagtga agacaaatct gataaatggt acccaaaaag aaggggcgtt
     4981 gatttttatc atcactataa agaagattta gctttatttg ccgaaatggg ctttaaaacc
     5041 ttgcgcctct ctattgcttg gactcgcttg ttccctacag gagaagagct ggaaccgaat
     5101 gaaaaagggg ttgcatttta tgaaaacgtc tttaaagaaa tgcaacgttt aggaattgag
     5161 ccgattgtga cactatctca ttatgaaatg ccattagctt tagcagttaa atataatggc
     5221 tgggtggagc gtcgagtaat tgatgatttt gttcgcttcg ctaacgtttg ttttgatcgc
     5281 ttcggcaaat acgtaaaata ttggctgaca tttaatgaga tagacagcat ccaccgtcat
     5341 ccatttacaa cagctggaat tatccaagag aaaagcgcag aaggtcaaga agctcaagat
     5401 atctatcaag cattgcatca tcaatttgtt gcgagtgcgt tagtgacaaa agatgctcat
     5461 gaaaaaattc caggaagcca agttggttgt atgttaacga aattgatgac ttatccatta
     5521 acttgcgcac cagaagacgt tgagctgacc ttgaaaaaga acttagaaaa taatttttat
     5581 gcagatgttc aagttaaggg agaatatcct gctctaatcg aaaaggattt ggaaaatcgg
     5641 ggaattcaga ttaaaatgga agatgaagat cgagaaattt taaagaaaca taccgtagat
     5701 tttgtttctt tcagctatta catgtccatg gcagaatcag ctgatccaaa cgcagaacgc
     5761 acaccgggaa atactgtttt aggggttaag aatcctcatt tgccttcaac agattggggc
     5821 tggcaaatcg atccaaaagg tttgaaaatt tcgttgatcg aactttatga tcgttaccaa
     5881 gtaccgttga tgattgtgga aaatggtatg ggagcagtcg atcaggtagt agatggtaaa
     5941 atccatgatc cgtaccgcat cgactacttt aaagcacact tccaacaaat gaaagaagca
     6001 atcgatgaag gtgtagattt gatagcttat actagctggg gaccaattga tttagttagt
     6061 gcaggaactt ctcaaatgtc taaacgatat ggatttattt atgtggatgc agacgatgaa
     6121 ggaaatggga catataaccg cttgaaaaag gattcgttca a
//
LOCUS       DAZUKC010000125         6068 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000125 DAZUKC010000000
VERSION     DAZUKC010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.130"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            832..1566
                     /locus_tag="ACG80T_002577"
     CDS             832..1566
                     /locus_tag="ACG80T_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4842185.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1582..2751
                     /locus_tag="ACG80T_002578"
     CDS             1582..2751
                     /locus_tag="ACG80T_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS4842186.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2767..3762
                     /gene="lacD"
                     /locus_tag="ACG80T_002579"
     CDS             2767..3762
                     /gene="lacD"
                     /locus_tag="ACG80T_002579"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFS4842187.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3841..4767
                     /locus_tag="ACG80T_002580"
     CDS             3841..4767
                     /locus_tag="ACG80T_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFS4842188.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4786..6036
                     /locus_tag="ACG80T_002581"
     CDS             4786..6036
                     /locus_tag="ACG80T_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4842189.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         2027 a          997 c         1246 g         1798 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccaa agtttgaaaa atgctatttc gaggtagaag gatattatat aagcacggtt
      241 gactaaacga agtgataata aaaaaagtta ttcaagaata agaaaaatta tgatacgaat
      301 tcagataaac tggagaatac agagaccttt aaaagggata agaaacaaca taaattcctc
      361 ttaagagaga acgaaaggac aaagccagca acttgataca cgagctagta ttagaggaat
      421 ttattctcat agactccctt ttgaatacgt ttcaacttta attttcatgt tcataaatga
      481 tttgaaacca aagcaaatgc gttgcacttt tcctccgtct tccgaaaatc gttcaattca
      541 ctggtaagta gtcatttgat agtttgaggc aaattgttga ttgatgaagg gtaggagcta
      601 aaaaaagata ccagttgaag agagaaaaag aaaaaaatag agggtgttct tgaatgaaaa
      661 aagttggaaa attatttttc atttcctttt attcctatta aagtcagtga tgtcaatcat
      721 attaacaaga ctaaaaatga aaataaaaaa aagattgttc ttagtattga ctcataaaaa
      781 ataccactac tatacttaca ttgtggttat agccagttag gagagacatc gatgcaaaat
      841 acctttcaat caaaatcact ttattatcag ctggtagaaa tattgaaaca acgaatcgaa
      901 aacagtatgg tcgctcatga caagctgcca tcagaaagag agttgaccag tttttatggc
      961 gttagtcgca cgacggtgcg actagcattg caagaactgg aaaaaatggg ctacatttat
     1021 cgacaacatg gaaaaggaac ctttgtttcg gaattaagga caaatgcgac tgatctagct
     1081 ggcgcatata gttttaccga acaaatgcgg gcaatgggca aacatcctga aaccaagata
     1141 ttggccttca agaagattga agcaactaaa tatttagcga gtcatctgaa tgttcaacta
     1201 ggtgagcctt tatttcaatt agaccgattg cgcttagctg atgatttgcc aatgatgatc
     1261 gagaaatcct atttgccagt gaagctattt atgtcccttt ctcaagaaat tctatttctg
     1321 aaacctttat ataatttgtt ttcagagaat ttcaatcaaa tcgtacggtt ggctgacgag
     1381 gaattttatg ccagcattgt ccagaaagaa gatagtgagt tgctaaatat tccagaagga
     1441 tcaccagtgc tccatttagt tcgctcaacc tataatctcg agaacgaagt gattgaattt
     1501 acattaagtg tagcgcgggc tgataaattt cattataaga tcaggcatct gcgttccact
     1561 aactgaattg gaggaaagaa aatgtttatg ttctcaaaag aagaactacg aaagctagga
     1621 gcagaaaata ctacccgaga aattcgacaa caaccggaac tgtggcatga gacgtttgac
     1681 cgttatcaag aaacagttgc agatttagat cgtttgttta cggatgcgaa acaacatgct
     1741 ggagagcgac caatccgagt catttttaca ggggccggaa cctcgcaata tgtgggagat
     1801 acactaattc cgtttcttac tagtcaagga aatacgaacc agtttatttt tcaaagtatt
     1861 ggttcgaccg atatcgttgc tacgccaaaa gaatacctga ttcctgatga gacaacgatc
     1921 ttggtctcat ttgcccgtag tggaaatagt ccagaaagtc tagccgcagt taagatggcc
     1981 aatcagctgg tcaacaagat ctatcatatc gtgatcacct gtgcaccaga cggattattg
     2041 gctacacaaa tgcctacaaa aaaagaaaat ctagttttac taatgccaaa caaagccaat
     2101 gacgctggtt ttgctatgac aagcagtttc agctgtatga cgctgactgc gctacttatt
     2161 tttgatcaag caagcaatca gcaaaaagaa agatatgttc atcaattaag ccgactagga
     2221 gaagaagtca tccaacgaga atcagagatc caagggtatc tggcatcaga ctttcatcgc
     2281 atcatttact taggttcggg aagcttaggc ggattgacta gagaagcgca attaaaagtt
     2341 ctcgagttaa ctgctggaaa aatcgcaacc gtatttgata catctttagg attcagacat
     2401 ggtcccaaat cttttgtcaa tgagcaaaca ttggttttcc tgtttgtcag caatgactca
     2461 tatacgcaga aatacgatgt agacatgctg gaagaaatca aaaatgacgg tattgcccaa
     2521 cgcgttgttg gaattggtca aaagggttca ttgtttagtg gtgattcctt tgaattagtt
     2581 gcagattcgt tactgccgga tggctatctt gctttagcag atatcatggt agcccaaacg
     2641 atcgcattac tttgttcggt gaaggttggt aatacaccag atacaccatc gccaagtgga
     2701 acagtcaatc gtgtggtcaa aggcgtaacg atttatccat atgaaaaata ggaaagagtg
     2761 tgaagcatgt tagaatggtc ggaaaataaa agacaagcaa tggaacgtct atcgacgcca
     2821 gaaggcatta ttaccgcttt ggcgatcgat cagcgaggcg cgctgaagaa aatgatgtcg
     2881 gctttaggga tggaagcgac aaaggaaaaa attgagcagt ttaaaacact aatttcccaa
     2941 gaattgactc cttatgcctc agcaatttta ttggatcctg aatacggact gcctgctgca
     3001 cagaagcgtg cagcagcgag cggtctgctg ttggcatatg aaaaaactgg ttatgatcaa
     3061 accgtacccg gccgcttgcc ggatctgctg tcggactgtt cagttctgcg attgaaagag
     3121 ttaggtgcgg atgcggtgaa atttttactg tattacgatt atgaagaagc agaggaaatc
     3181 aatcggcaaa agcaagtttt tgttgaacga attggaagcg aatgcatggc tgaaggactg
     3241 ccattttatt tagagttatt gtcatatgat gctaaaaatg tagatacgaa atcgctttcc
     3301 tatgccaaag caaaaccgcg caaagtgatc gaaatgatga aagaattctc gaaaacccag
     3361 tataaagtag atgtcttaaa ggttgaagta ccagtaaaca tgtcatttgt tgaaggaaat
     3421 gcaacagggg agatcgccta tacacgtcaa gaagctgccg cttattttaa agaacaaagc
     3481 gatgcaacag atttgccgta tatcttctta agtgctgggg tatcagcggc attattcatg
     3541 gagacattgc gttttgccaa acatgctggg gcacgattca acggtgtact ctgtggcaga
     3601 gcaacttgga aagatgcagt cgctcctttc gttgaaaaag gagaagcggc aactttagaa
     3661 tggctgacag aacaagggac acaaaacatt cgtcaattga atgaagtgat aagagaaact
     3721 gccatcccat ggtatgagaa agtaagtgaa agcacttgtt aatgtttcta atatattagt
     3781 agtgataaga gatatgtgtg attataattt atcattattg aaactgggag gatattttat
     3841 atgaataaaa gattgttaga ttattcagct tcagattttt tgaaagctaa gccgatggaa
     3901 ttaaagcagg caattttggc ttctgaaggt agaacaatta tggcagaaaa tgtgccaagt
     3961 agctgtcctt acatgggaaa atcagtgaca aatgcagaat tggagagagc tgctggagct
     4021 gatttaattt tgtttaatgc actagatctt tttaatcctc aaatttcagg tgtaccttta
     4081 actctaaaag agaatccaat aatctggatt aaaaaagctt taggaagagc tattggtgta
     4141 aatttagaac ctgtagacaa agaagttttg atgagtgaaa atcgaactga aatttcaagt
     4201 ggtagaatcg tttctcctga gactttaaaa aaggcaaatg aattaggctt taattttatt
     4261 tgtttaactg gaaatccagg aacaggagta tctaatagaa ctattgagaa agcaataaca
     4321 ttatccaaat caatttttaa tggattaatt atcgctggaa aaatgcatgg tgctggtgtt
     4381 gatgaaccaa ttatgaactt agatatagca aaaagattta ttaaaacagg tgtagatatt
     4441 ttattagttc ctgctccata cactgttccg tatttcatgg aaagtgattt gcgaggaatc
     4501 gttgattata taaaagattt taatagaaat aaatctattg aggaaaaagt tctagtacta
     4561 acggctaatg gaacaagcca agactcttct gaccaagaaa ctattaaaaa aattgcatta
     4621 gcatcaaaag catgtggagc tgatattcaa cacatagggg attctttaaa tggtatttgt
     4681 cttccagaaa atatttttgc tttgggtcaa gcaattcggg gatatcgtca tcaaattatt
     4741 atgttaggaa aatctaatta tcgttaatta aggaggaaat gaaagatgat cgacaaagtt
     4801 caaaaaatag ctgcaaaaat tagtaatcaa agacatctac tagctttaag agacagcatg
     4861 gctgcagtta tgccattaat cattattggt tcctttttta tgttattagc aaatttcccg
     4921 atagaaggat ttacaaaatg gttatctgaa attggagtac aatccttttt gaacaaagca
     4981 tcagattcaa catttggtat tattggatta gctgttacct actctttggg acacaatcta
     5041 gcaaagactt atggtgtaga ctcattatca gcaggattat tatctttgag tggatatgtt
     5101 ttacttacac ctttattgga tgcagatggt tctataggat ttccaacaaa atactttggt
     5161 acctcgggtc tttttgttgg tattattgta gcacttgtat ctacagaaat tttccggtgg
     5221 tttgtactta aaaatattac aattaaaatg ccggcaagcg ttccaccaaa tgtaagccga
     5281 gctttcgttg ctattattcc tggctttttt gtcgttctac tatggtttat tgttgttgta
     5341 atttgttata aacttggaat tgaaaatgtt catgctctta ttgcagatac actcgcaaaa
     5401 ccactatctt tactgacgaa aactttacca ggaattatat tggttatact tattcaatgt
     5461 ttcttttgga tgtttggtat tcatggtgca caggtaacgg ggccaataat tgaaccgtta
     5521 ttattgcaaa actctgatgt aaatagaatt gcttatcaag ctggaaaaga acttcctaac
     5581 attataactt atgaattctt atataacttt gttttttctg gtggtgcagg ttgtttattt
     5641 gcacttgcca ttttaacatt tttcttttca aagagtcagg aaaataaaac tttaggtaag
     5701 ttatcaatcg ctccagtaag ttttcaagta gcagagcccc ttctatttgg gtttccaact
     5761 atattaaata taaaaatgtt aattccattt gtaactgcac cagtagtaac aacattaatt
     5821 acttattttt caatgagtat gggattagtt gcaaaacctg taggagcaac aatcccatgg
     5881 acaactccac cgataattgc cggattttta gcttctggtg gacgaataag tggtgcagtg
     5941 attcaagtaa taacaattgc aataaatgtt ttgatatact atccattctt taaattagat
     6001 gataatgcca agctaaaaag cgaaaaaaat gattagaact acctatatgt ttctaactgt
     6061 agttaagt
//
LOCUS       DAZUKC010000126         6030 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000126 DAZUKC010000000
VERSION     DAZUKC010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.131"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..311)
                     /locus_tag="ACG80T_002582"
     CDS             complement(<1..311)
                     /locus_tag="ACG80T_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS4842190.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ET"
     gene            543..1535
                     /locus_tag="ACG80T_002583"
     CDS             543..1535
                     /locus_tag="ACG80T_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4842191.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            1595..3016
                     /locus_tag="ACG80T_002584"
     CDS             1595..3016
                     /locus_tag="ACG80T_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFS4842192.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            3009..4508
                     /locus_tag="ACG80T_002585"
     CDS             3009..4508
                     /locus_tag="ACG80T_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFS4842193.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            4547..5941
                     /gene="uxaC"
                     /locus_tag="ACG80T_002586"
     CDS             4547..5941
                     /gene="uxaC"
                     /locus_tag="ACG80T_002586"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFS4842194.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         1974 a         1111 c         1214 g         1731 t
ORIGIN      
        1 gtttctataa aatttagttc tttcctgatt tcagaataag tggagtaatc tgtatatgtc
       61 agcggatgct tgaatatgtc cgagaaattt ttggtcgaac catacgtaga aaacgaatat
      121 tcaatcgttc taattttcca ttcaacgtta gttttaactt gcctgagcaa attctttttg
      181 tctgttaaat aactcgattc gatcgattca taggtattct ggtaaatgaa taaactaaat
      241 cctatgacaa gtacggctcc taataaaaac atcggtaaaa atattttata aaatactgta
      301 cttttcttca ttttcttcct ctcctccttt taaaataaaa ggctttcatt tttctgtaaa
      361 attgatactc atctaattaa atgagcattc cccgctttca agaatagcat aattaaaaat
      421 aaaaatccaa gtaattttta acgtgtgcaa tcttttcata tcaaagatct attgacttat
      481 cgtagttatc gtaatatgat gaatgcgtta acaaaaagtg aaaaagtaag gaaaatgatg
      541 gaatggtaac gatcaaagat atcgctaaag cagctggcgt ttctcatacg acagtttctc
      601 gtgctttgaa taacagcagt ttaataaaaa atgaaacaaa acaaaaaata cgacaactag
      661 ctaaggaaat gaattattcg ccaaactata gtgcaaaagg tttagtgaat caaaaaaaat
      721 atctaatcgg actatttttc tcaagtttgc ataaaggaac ttcatcaaat ttcctagcag
      781 attccattgc aggcatccat tcagtattag acacaaatta cagtttatct gttgaaagta
      841 tcgatgttgt tgatttaaca gaaatcaacc tgcaacgcta cgatggactg atcgttatga
      901 gtcaaagtga tgatgatcag cctttcatcg actatctcaa agaaaaagaa tttcctttcg
      961 ttgttgtcaa tcgtcacatc aatgatccag cggttatcaa cgtcactgcc gatgatgcgg
     1021 ccggtgttat gcaggcagta gattacggta ttggatgcgg acatacgaag atcgcttata
     1081 ttggcggaaa gaaaaatttt cgttcgacaa aagaacggga aaaaggggta ttgaaaagtc
     1141 taaaaaaagc gagcatccct gtcaatctag cctatttttt aacaggagat tacagcttag
     1201 aaagcggcct ccaaaacatg gaatacctat tatcactttc tgacctgcct actctggttt
     1261 tttgcggcaa cgacgacata gcgattggtg cattgagagc tgtacattta gctggcctcc
     1321 gtgttcccca gaacatttct ctgatcgggt tcgatgattc tccagtcgtc tcctatctca
     1381 atccgccgct taccaccgta cgtaagcctt tgaaagaaat gtgtcaagaa ggaacaaagc
     1441 ttttactaga tttgatcgaa ggcaggacga tcgaacaatc taaaattgag ctctccacct
     1501 tgttgatcct tcgtgagtcg atcaattact tgtaaactac aagtaatttt atataaaaat
     1561 gttaacgtgt gcataacaca gaaaggaagt ttatatgaaa aaactgacaa aagaattagt
     1621 gaaaaaaaac gaagcacctg taaaagttct tcagtttggt gaaggcaatt ttatgcgtgg
     1681 atttattgat tggcagattc agcagctgaa taaccaacaa ttgtttaaag gtaatgttgc
     1741 aatcattcaa cctttagaac aaggtcttgg aaatatgatg aaccagcaag ataatttata
     1801 tacggtcatt ttacaaggac tcagcaatgg cgaaatcgtt gatacttccg agattatcac
     1861 cgtagtagaa caaatgatca atccttatga tgaatgggat gcttatctat ctctagcaga
     1921 aaatgacgac ttggcctata tcgtctccaa tacaacagag gcaggaatcc aattcgtgga
     1981 gactgataat ttaacagata ctcccccaca gagcttcccg ggcaaactga cagccttttt
     2041 ataccgacgt ttcaaattaa acaaagctgg ctttaccatc attccttgcg aactgattga
     2101 cagaaacggc gaacgtctaa aagaaatcat tttacgctac gcagaaaaat gggatttaga
     2161 acctgctttt gttgagtggt tagaaaaaga aaatgttttc tgttgtagtt tagttgatcg
     2221 catcgtccca ggttatcctc gcgaagatgc aggtgttttc aatcaacggc ataactatga
     2281 agatcaattg atggtcaaag cagaaccatt catgctttgg gtgatcgaag ggcctaaaga
     2341 attggagaat caactaccat tcaaacaggc tggcttgaat gtcatcatca cagatgatat
     2401 gactccttat cgtgaacgta aggtgcattt gcttaatgga ccgcatacag cgatggtccc
     2461 tcttgctttg ttggcggaat tggaaactgt agaagaagta atgaaagatc ctttattctc
     2521 cgcatatgtt gatcagttat tcaatctaga attgattcct atgctttctt tacctaaaga
     2581 tgaattagcc atttatgctg accaaatcaa agaacgtttc ttgaatcctt ttgcccatca
     2641 caaattagaa gcgatttcat taaatagtgt ttcaaagttc agcacccgtc tgcttccagt
     2701 gttcaagaag tatatcgaag aacaaaatca ggtaccaccg ttaatcactg tttcactagc
     2761 tgcgctactt cttatgtatc gaggcgatca agtaaagcca catgatgatg aaaagacgat
     2821 aagcgaattt actgatgctt ggtctgataa tggaacagct attcctcgat tattgcaaaa
     2881 cgctgcgctt tggggagaag atttgtctca aataccgaat gtgacagata cagttcaaga
     2941 aatagctgca aagatcgatc aaaaaggtgt ccggtcagta ttacaagaaa tgaaaggaac
     3001 gatggtccat gcttgaaacc ctgcaaccaa caatgaaatt aaatcgacga gacagtgtgg
     3061 tcgtcgcatt aacccctatt ccaaaacaaa cacaactgac gattgatcag caaacgatca
     3121 ccactcttga agatatcccg caaggtcata agattgcatt gggtgatttg aaagctggag
     3181 acaacgtgat caaatatggc tatccaatcg gtcatgtgac gacagacgta actgccggtc
     3241 aatggctgca cactcataat gtaaaaacca atctttctgg tgaattggat tatcgatatg
     3301 aaaaagatgt ccatccaagt cattaccttt ttgagaaccg aacatttcaa ggttacttgc
     3361 gaaaaaatgg aaaagtaggg attcgtaatg atttgttttt agtacctacc gttgggtgtg
     3421 tgaatggcat tgctgaactg atcgtcaaac agttcaagga aaaacatcca gatctgggac
     3481 agtttgacca tattacgatt ttaaagcatc cttatggctg ttcgcagctt ggaaaagatc
     3541 atcaaaatac tcgagaaatt ttagcggacg cagtaaatca tcccaatgcc ggaggagttt
     3601 tagtatttgg attaggttgt gagaacaata cagttcctga attcaaaaaa attttaggtg
     3661 aatatgatga agaacgagtg aaatttctag tggctcaaga tgtttacgat gagatcgaac
     3721 aaggtgtggc tctattagaa gagcttttaa cagcggcgga aaatgaccag cgcatatcag
     3781 tcccgctctc taagttgaat gtcggtttga aatgcggcgg ttctgatggt ctatcgggaa
     3841 taactgctaa tcctttactt ggtgcatttt ctgactactt gattgctcaa ggaggcagca
     3901 cgatcttaac agaagtgcca gaaatgtttg gcgcagaaca agtattgatg gcacgagcgg
     3961 aaaatgaaga agtttttgac tcaatcgttg acttgatcaa tgacttcaaa cagtatttct
     4021 tgtcttacgg ggaaccagtt tacgaaaacc catctcctgg taataaagca ggtgggatca
     4081 ctacattaga agataagtct ttaggctgca cgcaaaaatc aggaagctct cctgtcgttg
     4141 atgtgctaca atatggcgaa aaaatcagaa aaccagggct tagtttattg caagctcccg
     4201 ggaatgatct tgtagctgcc tctgctcttg cttcatccga ttgccagttg gtcttgttta
     4261 ccacaggaag agggactccg tttggctcat atgtcccaac ggtcaaggta tctacgaata
     4321 caactctttt tgaacgcaaa ggtcattgga tggattttaa tgcaggcgtc ttattagaac
     4381 aaccaatgga agtaatctta gaatcgtttg ttcaaaaaat catcgctgta gctagtggag
     4441 aagaaacgaa aaatgaacaa aatgacgttc gagaaattgc catttttaaa aacggagtaa
     4501 cactttgatt ttcataaaac agtataaaaa gaaagaagga agatttatgt ttttatctga
     4561 tgatttttta ttaaaagacg attgggcgaa aaagctttac cattcctacg caaaaaacat
     4621 gcccattatt gattatcact gtcacttaga tccaaaagaa atttacgaaa acaacaattt
     4681 cagcaatctg acagaagctt ggttaggagg cgatcattac aaatggcgct tgatgcgagc
     4741 ttgcggcgtg cctgaagaaa aaatcacagg aaatgcttct gattttgaaa aatttttagc
     4801 ttggtgtcaa accgtaccaa aaattatcgg aaatccgctt tacagttggt cacatcttga
     4861 attgaagcgt ttcttcggta ttgacttgtt gataaatgaa gaaaatgcag agaaaatttg
     4921 gacacaagca aatgaaattt tggcagctcc tgattttcgc cgtcgggaaa tcgcaaaaaa
     4981 atcgaatgta gaagtgattt gtacaacaga tgatcccgca gacgacttgc actatcatca
     5041 gttgcttgct catgaagaac cagatttgaa ggttctacct tcattccgtc cagataaagc
     5101 actcaatatt gaaaaagaag gatttaatgc atgggtcaca ctactggaag aagcagctga
     5161 ccaaacgatc accacctatc aagaactgat agaagtctta gggcagcgga tagaatactt
     5221 ccatcaacat ggatgtcgtg tttccgacca cgctttagac atcttacgct ataaagaagc
     5281 ggatgaaaca gtattagaag agatttttca aaaagcacga gctaacaaac tattatcttc
     5341 agatgaaatc gatgcttatc ttacagaaac actgatacat ttgacccact tctatcattc
     5401 tcataattgg acgatgcagc tgcacatcca tgcttatcgt aactgtaata cacaaatgtt
     5461 ggaaaaatta gggcccgata ccggctatga tggaatgaat gatctttctt tgactattcc
     5521 tctgcaaaaa ttattgaacc gtgcagaaga aacggatcag ttgccaaaga cgatccttta
     5581 ttcattgaat ccgaatgact atcctgcgat tttagctttg atgggctgtt tccaaaaaga
     5641 acagaatgga aaactgcagt taggttctgg atggtggtac aacgatactc gagctggtat
     5701 gcgcgatcaa ttgacacaat ttgctgatgg tagtgctttg gggaattttg tcggcatgct
     5761 gacagattca agaagtttcc tgtcctacac tagacatgaa tatttccgtc gtgttctttg
     5821 tgaattttta ggcgaaatgg tcatgcgagg agaagcacct gaagatgaac aacttttagg
     5881 aagtctagtt caagcgatta gttatacaaa tgctaaaaat tactttggct tttttgccta
     5941 attgattaat agtaacagta acgaatcaaa aacaggatag tgaaataacg gaatccgttt
     6001 atccacgttt tagagaagaa gacggaccaa
//
LOCUS       DAZUKC010000127         5863 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000127 DAZUKC010000000
VERSION     DAZUKC010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.132"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..751
                     /locus_tag="ACG80T_002587"
     CDS             <1..751
                     /locus_tag="ACG80T_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842195.1"
                     /translation="QGIIGNPSLNHRYRKGFYSLFKTLQQNRVAYYLLSEPKQIAASN
                     IFTAVMTLMCVMCFSIFSYLLVQFIRQNLTMSKLFKSLLDLLILGSMLTTLTDRFEQS
                     EKTVEGIKNFLTTIDQFINGYMYLFEVGFILTLFGCVFIKLIKRSIEKEMVIDLIPTL
                     SGYEYYVDFGKYEELIHTDIVHTSIHEVTVKGIRSTLKDGQETKTIDRRKEVYNFYKV
                     EASLRYGIGKLRLDGSNIQQNKPYVLGKVVI"
     gene            795..1064
                     /locus_tag="ACG80T_002588"
     CDS             795..1064
                     /locus_tag="ACG80T_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842196.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            1296..1592
                     /locus_tag="ACG80T_002589"
     CDS             1296..1592
                     /locus_tag="ACG80T_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842197.1"
                     /translation="MNVKIRDLDPKFISEIDRRCVELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQEKFDQLLEKFSEIQSYNTKILEEYVSQNNRIIEILIEQNRGSEL"
     gene            1594..2016
                     /locus_tag="ACG80T_002590"
     CDS             1594..2016
                     /locus_tag="ACG80T_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842198.1"
                     /translation="MAKVIDKHIYFHQTHIDVLDETISTHAEVKNYSEAVRFLCMNYD
                     PEVEKKVNSNQAKLNGMSKEISILTEMVSEFADLHLKDTGILIGKNSTVYQEAKERVE
                     GKIKSNQTKKYSPQLKKPVNESVHARVDPFDPTKILKR"
     gene            2035..3627
                     /gene="mobP2"
                     /locus_tag="ACG80T_002591"
     CDS             2035..3627
                     /gene="mobP2"
                     /locus_tag="ACG80T_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFS4842199.1"
                     /translation="MTQSPSVILTCQFTLPNKKSFSTYIDYITREQALEEKERRTPEE
                     EQELQRIKNALEEFYIPEGETFSESKTGKEFSETSNEAQSLMEHGINFDKLEEQDFTN
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSIASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWNYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            3648..3968
                     /locus_tag="ACG80T_002592"
                     /pseudo
     CDS             3648..3968
                     /locus_tag="ACG80T_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4129..5270)
                     /locus_tag="ACG80T_002593"
     CDS             complement(join(4129..5042,5042..5270))
                     /locus_tag="ACG80T_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4842200.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            5518..5712
                     /locus_tag="ACG80T_002594"
     CDS             5518..5712
                     /locus_tag="ACG80T_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842201.1"
                     /translation="MVHSTIGVVVEKSRDNLVFVTEIQTGRSFVVTDKAAKAYQSSDI
                     LTLNLTTKTFVDATEDYPFV"
BASE COUNT         2062 a          927 c         1173 g         1701 t
ORIGIN      
        1 tcaaggtatt attgggaatc ccagcttaaa tcatagatac cgtaagggat tttattcttt
       61 atttaaaact ttgcaacaga accgggtggc ctattacttg ttaagtgagc caaaacagat
      121 cgcggcgtca aatattttca cggcagtaat gacattaatg tgtgtcatgt gcttttctat
      181 ttttagttac ttactcgtcc aatttatcag gcaaaattta acaatgagta aacttttcaa
      241 aagtttattg gacttactta tcttaggcag tatgttaacg actctaacag atcggttcga
      301 acaatctgaa aaaacggtag aggggataaa gaacttctta acgacgattg atcaatttat
      361 aaatggatat atgtatttgt ttgaagtagg ctttattctc actttgtttg gttgtgtatt
      421 tataaaactc attaaaagat caatcgaaaa agaaatggtt attgatctaa ttccgacgct
      481 gagcggctat gagtattacg ttgattttgg aaagtatgag gagttgatcc atacggatat
      541 cgttcacacg agtatccatg aagtaaccgt taagggcatc agatctactt taaaagatgg
      601 acaggagacg aaaacaatcg accgaagaaa agaggtctac aatttttata aagttgaagc
      661 aagtctacga tatggcattg ggaaattgag gttagatggg agtaatattc aacaaaacaa
      721 accatatgtt ttaggaaaag tggtgattta gaacttccta tgaaagatga ataaataagg
      781 agaatctttt gaaaatgatt cttctttaca gaattgtgct tatagttggc acggagttaa
      841 aaatcttaaa gaaggagcag ttattcaaag tgaaagaaca tcataatcag ttactatgta
      901 ttaaaacggg tacgatgaac attcaatacc gaacagaata tttagaatta acgaatcaaa
      961 ctgatttttt gtcgattcga gagcaacata agcaagaaaa attaatggct acttatagcc
     1021 aattgattga tgagtgggaa caattcaagg aatctaaaac ataaaattcc atttaaacac
     1081 cttatttttt aaagagcata aggaaacgtg accccacttt taaaaaaagt ggttggatag
     1141 gctgtgccta tcacgtttct gaaaaaaata aatgacccca aaataccaat atataggata
     1201 gtacaaggat agcaaaggat agtaaaagga tagaggtagg atagcgaaaa ggataggatc
     1261 cttttcacta tcctatatta tattggagga tatttatgaa tgttaaaatt agagatttag
     1321 atccaaaatt catttctgaa attgatcgaa gatgcgttga actaagtaat cgtacaggaa
     1381 ataaatggag tcgtaatgat tatttaaagc tactcgttga aaatgatttt gatcgtcctt
     1441 tgatggaata caaacaagaa aagtttgacc agttattaga aaagttttca gaaattcaaa
     1501 gttataacac aaagatatta gaagaatatg ttagccaaaa taatcgtatt atagaaatct
     1561 tgattgagca aaatagagga agtgaacttt gatatggcaa aggttataga caaacacatt
     1621 tactttcacc aaacacatat cgatgtattg gatgaaacta tttctactca tgcagaggtt
     1681 aaaaactata gtgaagctgt tcgcttttta tgtatgaact atgatccaga agtggagaaa
     1741 aaagtaaatt ccaatcaagc aaagttgaat ggaatgagta aagaaatttc tattttaacc
     1801 gaaatggttt cagaatttgc ggatcttcat ttgaaagata caggaatatt aattggtaaa
     1861 aattcaactg tttatcaaga agcaaaagaa cgagtggaag gaaaaattaa atccaatcaa
     1921 acaaaaaaat attcccctca gttgaagaaa cctgtgaatg aatctgttca tgccagagta
     1981 gatccatttg atccaacgaa gattctaaaa agataaaaat gaaggaggat aataatgact
     2041 caatcgcctt cagttattct cacttgtcaa tttactttgc cgaataaaaa gtcattttct
     2101 acttatattg actatattac aagggaacag gctcttgaag aaaaggaacg acgtacgccc
     2161 gaagaagaac aggaacttca acgaatcaag aatgctttag aggagtttta tattcctgaa
     2221 ggagaaactt tttcggagag taagacgggt aaagaattca gtgaaacgtc gaatgaagct
     2281 caatcgttga tggaacatgg aatcaatttt gacaagttag aggaacaaga ttttaccaat
     2341 tatctttctt atatgacacg tcactatgcg ttagaacaaa agaaagaact gacgcaaagt
     2401 gaacaaaaag aataccaacg cttgaaccaa gcaatgaaaa aatatcaaac agaaccccat
     2461 aaaaaagctt caggaaatct tcctggagtt ttttctatag cttcagacga agtgaaagga
     2521 agtgatctga aggagattaa gcaaatcttc caaagaggac aacaaaaagg ctcaatcatt
     2581 tatcaagatg ttgtttctca tgataattcg tatcttgaaa aaataggatt gtacgatccc
     2641 aaaacagata ctttagatga agaagggtta aaagcagctg gaagaaaaat gatggagaca
     2701 ttattcgaga aagaacaatt gaatgaaaca ggttactgga tggcaacgat tcaccgtaac
     2761 actaaacata tccatattca ttttgcagtt gttgaaaaag aaaatacgcg taaaccttac
     2821 acggaggttg aaaatggtgt gagttatatc gtaccgaaag gtaagagaag acaagcaaca
     2881 ttagacgcaa tgaagaccaa ttatgtacat gagttagaac gtttttctta tgaaaaggat
     2941 cgaatgattg gtatggagaa acgaaattta ttgacgcgga aaagtgattt gcgtaacacc
     3001 ttaacaaaag tggtaaaaga gccagcgcgt tacgatcgac acgcgatgaa cttattaaat
     3061 gaagtatata aaaccctacc tgacaaacgt tcggaatgga attacgggga tgaaaaacga
     3121 accaaacttt caccatacac aagagagaaa ctcgatgagt taaccaaata tgttttaaca
     3181 aaagaacctg aatatcaaga atatatacag atttcaaaag cattgaaaga agaagcaaaa
     3241 agtctttatg gggaaagcaa gagagaatca aaggatgcag agaaaaatgc tctctttgat
     3301 ttaaaaaagc gcacaggaaa tgccatccta acagagctga agagacagga tagtcaaata
     3361 aaaccattaa tagaaagtgt tgatcagatg caatctattt acaatgaacg atcctttcga
     3421 acgcttaaac aattgccaac gaatgaatac aaatatagtt acgaagaatt catgaagcgc
     3481 aggaaacaat tccaacaaag agttgtcacg cagcggagtt tgcgtcaatt aaatcgaacg
     3541 attaatgccc atgaagaaaa ataccacgca agtaaagcat acgaagagca acaacgaaga
     3601 attcaaatgg aacaagaaag acagtaaaga cagtaggagg aatagcaatg aaacaatatt
     3661 tagatcaatg gaaagttata gaaggatcat taagagaaga acgaatcgaa caacttcctg
     3721 attgtttaga aaaagagcat ttgttccaga taagagaaat gttgagaaac gaacagtttg
     3781 atccaaatca atttttagtc gttgagtacc cagctactgg tgtgtattgt tgtaatcatg
     3841 tgaatggtga aaaatatttt atcattcaag aatatgaggg caagttagca ccttattata
     3901 ctacttggga aatgaatgaa gagggaatca ataactttcc atgtaaaagc attggatttg
     3961 agtcaatacc tcggacacga tttgtgagaa ttttttcgag attccagcgg gtgagtatct
     4021 catcgtttcg accagcggtc aaaccaaaac cgtttgaccg ctgatcgaag cgatgaagtt
     4081 gaaaccctgt ggaaatctcg ccaaaaaaat gtaagtggta gctaggaact aagcttgcaa
     4141 tgccaactcc tccatctgat ttggtgttaa atagttgata ctactatgga ttcgcttgcg
     4201 attgtaaaat ccttcaatgt attgaaataa cgctaaataa gcagcttcaa agttttcata
     4261 cgcttttgat ggatagactt cttctttttt gagtgaggcg tgaaacgatt cgataccggc
     4321 attatcgtag ggacagccct tacgactgaa ggagtgccga atgttcaatt cattcagctt
     4381 tgcctcatag gcttcgctag tgtactgaga acccaaatct gagtggatga tcaagttctc
     4441 ggagggtgtt tggtttaaga gcgcattgtc aagggtttcg ataaccaatt ctttcgtcat
     4501 ctgcttgcca aacttccaac cgatgacttt ctttgtgtgg aggtcttgaa tagtggagag
     4561 gtaggtccag ccatctttta gggtatgaat ataggtaata tctgtcaccc atttttgatt
     4621 gatcgacgta gtggtaaaat cttggtttaa caggttttta cgtccttcac ttttaacttt
     4681 cgcttgtttg ccaggcttgt acttcttgag ggtgactgaa cgcaagccta attttctcat
     4741 cagtatttgt actttcttta aggacaaggt ctcgccacgg ccaacaagaa tcttatgaat
     4801 tttaggcgca ccatagatac ccttgttttc aaagtagatt cctgaaatca agcggtttaa
     4861 gcgtttgttt tctgattggc gcttggaagg tttctttttc ttccagtcat agtagctgga
     4921 tttcggtgct tccaaaacgg tacacatttg ttttacgtca tgatcatgaa catttgattg
     4981 aataaattcg aagacatcac tcatttttac ttttgagcga atatggttaa agcctttttt
     5041 aggatagtgt tttcctcctg catgcgggcc atttcctttt tcatcgctag gatttctgcc
     5101 tcagtcatgc cggttgctac attctttgta tgaagcttct tccaacgata aatcgtttgt
     5161 gtagcgatac catattgcgc tgccagttct tcgaccgatt ggccagactg gtttaaatct
     5221 aagatcatct tcttaaagtc ttcatcaaat tttttcttgg cttttgccat cgtggacacg
     5281 tccttttctt agtagttaga ttctaccaaa tcgtgtccat gaaatcatcc taacatcaca
     5341 ttgaagagtc catttcactc acagagtgtt agttgttaag tgaataaaag aagtaaaaga
     5401 gtcctgaacg tgattgtcca ggactctttt tttcaatgaa ttgttttccg ccaactcatt
     5461 gagtggtgcg atttgcgact acattatatc aaaagaaaga gaagggagga agcagaaatg
     5521 gttcattcaa cgattggagt tgttgttgaa aaaagcagag acaatctcgt ttttgtgaca
     5581 gaaattcaaa caggcagatc ttttgtcgtc acagacaaag cggcaaaagc ttatcaaagt
     5641 agcgatattt taacgttaaa tctgactacg aagacgttcg tcgatgcgac agaagattat
     5701 ccatttgttt agtctaatgg tgataagtca agatgaaatt taagattttc tttattgagt
     5761 aatatgtttt gaagcgcacg aaactagacc cgatcaaaat caaatttttt tcgggttgat
     5821 tcaaggattt gcgtcaataa cggattgtta tttctctatc aca
//
LOCUS       DAZUKC010000128         5752 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000128 DAZUKC010000000
VERSION     DAZUKC010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.133"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            141..755
                     /locus_tag="ACG80T_002595"
     CDS             141..755
                     /locus_tag="ACG80T_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4842202.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(779..1129)
                     /locus_tag="ACG80T_002596"
     CDS             complement(779..1129)
                     /locus_tag="ACG80T_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS4842203.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1123..1386)
                     /locus_tag="ACG80T_002597"
     CDS             complement(1123..1386)
                     /locus_tag="ACG80T_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFS4842204.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            complement(1727..1906)
                     /locus_tag="ACG80T_002598"
     CDS             complement(1727..1906)
                     /locus_tag="ACG80T_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842205.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            complement(1922..2122)
                     /locus_tag="ACG80T_002599"
     CDS             complement(1922..2122)
                     /locus_tag="ACG80T_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842206.1"
                     /translation="MNKIIPLLFVLFVGEILMRHAAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKMIAAIFFIGLFR"
     gene            complement(2287..2994)
                     /locus_tag="ACG80T_002600"
     CDS             complement(2287..2994)
                     /locus_tag="ACG80T_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842207.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
     gene            complement(2987..3424)
                     /locus_tag="ACG80T_002601"
     CDS             complement(2987..3424)
                     /locus_tag="ACG80T_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842208.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            complement(3439..3690)
                     /locus_tag="ACG80T_002602"
     CDS             complement(3439..3690)
                     /locus_tag="ACG80T_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842209.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(3690..3896)
                     /locus_tag="ACG80T_002603"
     CDS             complement(3690..3896)
                     /locus_tag="ACG80T_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFS4842210.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            4474..>5752
                     /locus_tag="ACG80T_002604"
     CDS             4474..>5752
                     /locus_tag="ACG80T_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFS4842211.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQ"
BASE COUNT         1731 a         1122 c          973 g         1926 t
ORIGIN      
        1 cacatactat ttcctccatc attagttttt gtctatataa ggtataccct aactagacac
       61 gaccgcgaca ttgaaaaatc tggttttctc gtgtatgatt agggtgtttt ttaaatagtt
      121 gcagaaagta ggtaaaaatt ttgagagttg catacatacg tgtctcatcc atcgatcaaa
      181 atgaacaacg gcagattgaa gaaatgaaaa aattcggtgc agagagaatt ttcattgaga
      241 aacaatctgg cgcaacgatt actcatcgac ttgtttttca agaagcactg gatttcgtcc
      301 gagatcaaga tatttttatt gtggaagcta ttgatcgact tggaagaaat tacgacgaga
      361 tcatcgcttc agtcaattac ttgaagaaga aaaatgtgca gctgatcatc acaagtctac
      421 cgatcatggc cgaagcaatt ggcaatcctc tcctggataa attcattaaa gatttgatca
      481 ttcaaatttt ggccatgatc gctgaacaag aacgtaccga atctaaacga cgtcaggcac
      541 aaggcattaa gattgcaaaa gcgaacggtg tttataaagg acgtcctaag ctatacagtg
      601 ccaatgcaaa agatccccaa cgtcgtcttg tttataaaaa catcgttgag gatctaaaaa
      661 aaggtgttgc gatcgctaag attgcaaaag actacaatgt tacgagacaa accgtatata
      721 gaataaaaaa agacagcatg gtcaatcacg aatagttgat catgctgctg tttctatttt
      781 atttgcttac tttttgaaaa actgttctaa ctttcatcaa taactcctca actaaactat
      841 catgcgcttt ttccaaaaaa atacactctc tggcttcata atcaattgtc gttagttgat
      901 ctaataatac ttttcctgtt gtcttcattt catattctga gtttatattt acatatagcg
      961 gatagtttct agttgtacta gaaattggag caactgtaac aacgttacta taatccgcaa
     1021 taatatcatg acttaaaaca atataaggtc ttttacctgt ctgttcgtgt ccagatcgag
     1081 gtgctgtatt caataataga atatcgcctt gtctaggttt tctcaccatt tttcattacc
     1141 tactgcttca cctaaatttt gaatcgtcac atgatttctt tcatcagtgt aattttcaaa
     1201 taactgttca attgttaatt cattttctat attagccgct tcgataatta gccgcttctg
     1261 accgtttact tcttcttgta ttatcttcac tttgtcttct gtatgaattc caagatattc
     1321 cataatatac tttggcaatc taaggccatt actatttccc cattttttta taggtaacat
     1381 ttccatagta gacacctctt tctgtatata catagtataa catacataag aaaaaacgta
     1441 tatacttaat tatccttttc tattaaatta gattaacatt ccatgaatcc ataaactatg
     1501 ttaacattta tatgaaaata gtcgaaagga aatcataata tgtttaagaa aacaaaatct
     1561 ttagaatcag gtaagaaaaa aattgatcta aaaataatct cttctggata ctttaaagat
     1621 ttcatagtta ttgatacaaa atggaataaa aaggacatag aaaatattct aaatagtagg
     1681 aacaattaag tgctatcact tttgtttctt caaatgaaaa gtgacgtcac ttttttagtg
     1741 ctggtatatc aaatcctgct ttttcttgcg cgatcaatag atctaacgca atacacataa
     1801 tcttagattc actttttccg tacttctgct ttagttgctg caattctttt tgtctctttt
     1861 catctaatgt cacgggtaac cgtttatttc ctattgcaat tggcattagt actctctcct
     1921 ttcatcgaaa taaccctatg aaaaatatgg ctgcaatcat cttgaatagt agactcacaa
     1981 gggctaatac aaatcgccaa aagatcaaac aaaataccag tattcctaag aatactacga
     2041 agtttaaatg tacagctgca gcatgacgca taagtatctc gccaacaaac agtacgaaca
     2101 atagaggaat gattttattc atatggatct ccttctttct cttaggttat cgggctttgc
     2161 ccgcttattt cgcaccacct aagcggcggg ggcggttgga gcgattggtg cgaaataaga
     2221 accattccct taatttcaag gttttttgtt cttatttcac acatcctcac ttttcggtat
     2281 aaatggttat ttaccttttt tcctttcaaa tcgttgatat ggcattcgtt tacggtcaag
     2341 ttcattttgg atttcttctg tatcgtcgta agtgtaggtg tctaaaaaat cagttaaagt
     2401 cgtgtaccag tcgtcgcctg ggttatctac ttcttctaca tattccctca aaaatgcttc
     2461 gggtacagcg agttcataat ccattcccat taacacgata gattccgtcc cacacgtcaa
     2521 aaacctagta aatacaaggt aataatcgtc taccgtcgca tttttgctgt ttagctcttc
     2581 catttcttct tccattaatt ccatgctctc gaaaattgaa taatattctg attctgagta
     2641 atagtgtttc ctacaaatcg tgtagctgac aaagttttcg accagctcac tcctttctag
     2701 gtacacattc atgatgtatt tttctgtttc tagtcgtaga agctctgttc ctttttttac
     2761 cgtttcagga cgacgtttat acacgtctaa atgattatgg ctgactgtga ccttcgtttg
     2821 acttgtttcg ctttccatat acaactgttc ttccactctt tgaataaagt acttcatttc
     2881 ttccatacaa ctaaagtctt tttgaatccg tctgattact tcattcaagt gttctttgct
     2941 tgttaaaaat tgcgaaacat cttctgttgt tagctgataa accaccttat tcattatctt
     3001 tctcctccga ttcaatcgta taaaatgcac catttttttc gatactttca aaagtatcta
     3061 gccagtcgct agccattgct ttagctttct taatctgtgc gaattttgca taagtcagtt
     3121 ctaaggcttg atattcggaa ggtttgatag tgcttggttt gacacctact tccaagcata
     3181 gtaaggcttg gaaaaaccat tttccatttt gttttttcag ttcttccagt gttcgatagt
     3241 acgtcgcaat gagattcatt tcgacacctg attcaatcat ttcttccact gcgactgaca
     3301 atttttcctt gtaatgtttt ttgggtacac gtgtttcatg gtataggaac gtcatatcct
     3361 ccattttttg tgccatttta tccaaaggta atttgacaaa ttcgtcatag taattttgag
     3421 ccatattttt tctccttttt agttctctaa tttggttaac caatattcga tttcataagt
     3481 ttccatgttt tcttgataat gcagttcata tcctgcaatc ttcactaggt cttcaaatag
     3541 catagtcggt gcatcctcta ctaaaggggt ttctatcgtc cagtaccgcg gaataagtaa
     3601 agcttctgct actttttcag aaatgacttc tttgtccacc aaaatttgaa cgacttcttt
     3661 tacagtcaat aatttgtgtt gtgttttcat cagcgtttct ttcctttctc atcaatcaaa
     3721 atgtaatcgc cataagacaa ggtatatttt ttcccgcatg ttggacattg atacaaacga
     3781 tagcttttga tacatttcgc tctttctcca caatcgcatt caacagtatg tggtgcatga
     3841 tagtttggga ataattcgct gaagggaaca ccccaataat caaatttctt tcgcatgttt
     3901 gattctgcct cctcttagga aaggttttac ttcctttcgt tagttctttc tttattgatt
     3961 tgttggccag aataaatcaa cgaaaagcga caaggacaag ggcgcgcagc gtgccattta
     4021 cccttgtcct tgtcgctttt tggtgataaa aggcaacttg ttgccggtct ggcaaatcaa
     4081 taaagagata acgaacagga acgaaccttt tggtgagagg aagttttgtc tattttgtct
     4141 tgctttgcaa gacaccatat cttagtgaac gtgcagcgtt cacttccttt cttctcgaac
     4201 ggcggtggcg gttggagtga gagaaaggtt tttcaattga gaaggcactt gtgtcttcct
     4261 aaaaaggtct tttcctgttt ctatgtaaaa caaaaagagc tgcaatcctc atttaggact
     4321 acaactcttc ttttattggc tctataattt atgggactga tgaatcagaa ttgattcttc
     4381 agccccattt tcattttagg tattaaaaaa catatcgttc tctagtatga ttaaatcacc
     4441 acaaaataaa caactgagag gacgatacgc tatatgaatg attctatcaa aaaaatgctg
     4501 agaataacag agaaagattt gatgattaca gaggtctctt acgagaccct tcagaagaaa
     4561 aagacgttgg tcgtcgatgc tgttctctcg cctactcctc gtgcttgtag aagttgtggt
     4621 tctactgtgg tagatggaaa cgggaaagca attatagtga aaaatggaaa aaaggaaacg
     4681 attgtccgtt ttgaacaata caatcatatg cctttggtta tgcgcctaaa aaagcaacgc
     4741 tatacctgta aaaactgtcg cacccattgg acggctcaaa gttattttgt ccaacccaga
     4801 cattcaatcg caaatcatgt tagatataaa attgcttctt tactgactga aaaagtatct
     4861 ttatctttta ttgcgaaaag ctgtcaggta tctttgacca ccgttattcg tacattgaaa
     4921 gagtttaaaa gctatttacc aaagcaatct aagaagatcc tccccagagt attgatggtt
     4981 gacgaatttc gttcgcatgc ttccatagaa gataagatga gctttatttg cgcggatggc
     5041 gaaacaggaa aattaataga tgttttgcct acgcgtaaat tacctcgatt aacaagctat
     5101 ttcttaggtt gtaccaatcc agaagaagta gaattcttgg tgacagacat gaacgccgcc
     5161 tacttccagc tcaccaaacg tgttctgcca aatgcgaaag tcgtgattga tcggtttcat
     5221 attgtcaaac acatgaatca agcgttcaat gagttgcgta tccgtgaaat gaatgaactt
     5281 cgtaaagccg gacagaaaag ccaggcagaa aaactgaaaa agaactggcg ctttttgcta
     5341 aaaaatcgtg caaacatcaa ccattatgaa tacaaaacat ggaaaagttt ccgagcacca
     5401 aaatacccat ttcttactga agcaatgatg attgatcgat tgcttgagtt ttctccgccc
     5461 ctaaaggagg cgtatccctt ttttcatgaa ttagttgaag cctttcgaga caaagaccct
     5521 gacttatttt tctccttatt ggcagaactt cccgaaacgt tagatgacag ctttcgggaa
     5581 aagcttcaaa accttctgac ctatgaagaa ggcatcacca acgcaatgat ctatccttat
     5641 tccaatggaa aaatagaagc gaagaatacc cacataaaga caatgaaacg agtatcctac
     5701 ggatttaaat catttgagaa catgagaatt agaatctttt tgatcaatca at
//
LOCUS       DAZUKC010000129         5727 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000129 DAZUKC010000000
VERSION     DAZUKC010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.134"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            196..921
                     /locus_tag="ACG80T_002605"
     CDS             196..921
                     /locus_tag="ACG80T_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842212.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            911..1420
                     /locus_tag="ACG80T_002606"
     CDS             911..1420
                     /locus_tag="ACG80T_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842213.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1490..2938)
                     /locus_tag="ACG80T_002607"
     CDS             complement(1490..2938)
                     /locus_tag="ACG80T_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4842214.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2938..3654)
                     /locus_tag="ACG80T_002608"
     CDS             complement(2938..3654)
                     /locus_tag="ACG80T_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFS4842215.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3635..3985)
                     /locus_tag="ACG80T_002609"
     CDS             complement(3635..3985)
                     /locus_tag="ACG80T_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFS4842216.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            4129..4902
                     /locus_tag="ACG80T_002610"
     CDS             4129..4902
                     /locus_tag="ACG80T_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4842217.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            5073..5439
                     /gene="ssrA"
                     /locus_tag="ACG80T_002611"
     tmRNA           5073..5439
                     /gene="ssrA"
                     /locus_tag="ACG80T_002611"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1662 a         1124 c          994 g         1947 t
ORIGIN      
        1 aaaattattg tcgctgctcc tcttttcgaa taaacagtgt tcaaaagctt gcatctacgg
       61 taatagaata gggcagcttc ttcacaacct cttgcttttt acaaaatatt ttctggtttt
      121 aattcgtgat ttcactaaag gaatttcata ctttattcat attcattttc tatacttttt
      181 atagaggtga aaagtatgaa aataatccgc tatgctactg ctcgattaat ttattacaaa
      241 aagcagacaa tactctattg tttattttca agcttttctg cttttttatt gatagcatgc
      301 ctgactcttt accattcgca atcggcttta gccaatcaag tccaagatcg gctttcattt
      361 attggcgctt ctgcatctac agagcttctt ccggctttag aattagctag ccgattttat
      421 ataaaaggca tattcagttt attttttcta tttgccttcc tttttatctt tttcttttac
      481 tattcagtca ggcaaaacaa gcaggaattg atcaattggc gcctgactgg tctttctaaa
      541 accaaattat tcctttttat tttctggcag ctgttctttc ctctctttct ttgttgtatc
      601 ttggttttat tgtggatcgt cagcttccag tcagtttatg aagcgcttct tcaaaggatc
      661 aattttcttg tcttaaagac tactgaactg ccagatatcc gtgatatctt gacttcggta
      721 agcggactta caattccgat ggatcaacag acttttttta ccatcgattt ccaaaatgat
      781 ctttttttca cagatatact taagggattt gcccaaactc ttattgggct cagtggatgc
      841 tgtctcattc ttagtctatt ccctttcact ttttttatcc atcatttgca aagtaggagg
      901 cctggacgta atgaaacctg aatatttgat tcatgctgaa cacttgaccg ttattttgtg
      961 tcctctcgaa aaacagcata aaatattgga gcacttgaaa aaagaggttc ttgttgcaaa
     1021 actggataat tttgcgatca tccaaaaaaa ttggccgatg tttccttatc tgaatcttaa
     1081 agaccatgtc ctgttggatg taccggaaaa agaaatcaaa caagatcgtt tggcctatca
     1141 agaaaaactt gatatctctc cttccttgct taattgtgcg gcagctgagt tgacgtcttt
     1201 tgaaaaagta aagctgcaat tacttcatgc cttgctttcc aagcgaaaca atattgttat
     1261 tgaagataca tttgatgaac ttagtgtatt ggagatccaa gagctactcc acttgctttc
     1321 ttatttagct catgaagaaa atcaaggtat cttactcttt actcatgatg ctactattgc
     1381 tcaaagtcct tatattgatc gtttagagcc cgcgggctaa atcaatcatt ttatagtaaa
     1441 aacaagcggt atagaagatc gattcatgat cttttatacc gcttgttttt tactcttttt
     1501 tgatttctac gtcaggcaaa cgaacagcga attttgttcc ttttcctagt tcgctttcta
     1561 cttctaattt tcctttatgc aagcgtacaa gttgctgcac gattggtaaa cctaatcctg
     1621 attcaccata ttttgtattt tttcttgaag gatctacttt ataataacga tcccagatac
     1681 ttttcatttg ttgttcagac attccaattc ccgtatcttc aatcgtgatg atcgtttcaa
     1741 gatagccttt ttctaaagta atcgtgattt ggccgttttc agtaaactgt atcgcgtttt
     1801 gaataatatt gaccatgatt tggacaaatc ggtcataatc agcgtaaaca tcaatatcat
     1861 caacggcttt caatgtcaat gtatctcctg ctgcttctgc tttagcttct aactgtgcaa
     1921 caatattttt caatgtttct gttccattga attttttaat gaccatactg atttgattcg
     1981 tacggatttt ttcataatcc aagttttcat tcaccaaacg gatcagacgc tctgtttcat
     2041 ttttcatcaa tttaatcgcg ttttctcgct gattttcagg gattgcattg tattctaacc
     2101 cttccagaag tccgttgatt gtcgtaagag gtgttctcat ctcgtgagaa gcatctgcca
     2161 taaattgtct tcgacgattt tcttgttctt caatttctgc ttgagattct ttcaatgaat
     2221 tcgtcatttt attgaaatca tctgctaact catcaaattc atctttgtca tgtacgggga
     2281 gctgtacatc aaaattacca ttcgtcactt ctttcgtcgc attccttaac cgattgatac
     2341 gcttaacttg gaatgctgca aaagcatagc tggcaataac tgcaacgata ctggaaaaaa
     2401 taaatccttt gaacaggttc agtgtaattg gacgaacgct gttatcgata tttcgcgcag
     2461 gttgactaac tacaagtgct ccatagaatt catgattcaa attaaatggc acaagtgcgt
     2521 aagaggtcgc ctgtgcttgt cctaaaatat ttttattcga tgtgaatttt tcttgacgtc
     2581 cattcctcag atttttccat tgatcagctg ttaaggtaaa attcagtaaa gctgtattag
     2641 tcggataaat gacacgttcg tttttatcga taaatataaa gttcacattt tgttctgtca
     2701 gtacttgctc tgtcagcagc aatgcatttt gaaacgcctg atcttgacta aactgcggga
     2761 gcgtatcggc ataggtttgc gttgtctttt caactgattc agcatatcca aacagttgct
     2821 ggtaattgtt ttcttcaatc gtttgttttg tcagttgtgt aaaagaaact ccaacgatca
     2881 ataagatcaa cgcgaccatt ccgcaaaaag ctaagagttg ctgatacaaa taacgcatta
     2941 ggcaacacca gaatcatcga atttgtagcc gactccccaa acagtttgga tgacttgtgg
     3001 tccgaccttt tcgatttttt gtcgtaattt cttgatatgg gcgtccactg tccgttcatc
     3061 accaaagtac tgataatccc agactaattc aagcaactgc tcccgagaga atacttgacg
     3121 tggttttttt gctaaagtaa acaatagatc gaactcttta ggcgtcagtc cttcaattgg
     3181 ccggttgtcc agatacgctt ctcgtgtttt cgtattcatc ttgaaatgac tagtctcgat
     3241 atcaaaatga tcatctgaaa ctgctgcttc ttctcttgct tctgccaact cactacggcg
     3301 atgcaaagct ttcattcgag caataagcgt cagaggactg aaaggttttg tgacataatc
     3361 atctgccccc atctctaagc cgatcacctg atcgctttca gaatctctag ctgtcagcat
     3421 gataatcggc acagtacttg aaactttgcg gatctctctt cctactagca taccgtccag
     3481 tgtgggcaaa ttcaggtcta gtgtaatcat atcccaatgt tgtgggtttt ctaaaaaggc
     3541 atcgagccct tcttttccat catacttgaa agttgcttcc caaccttcat ttaagaaaaa
     3601 catctgcatc atttcagaaa cagattgatt atcttcaatc attaaaatat tcattatcgt
     3661 tccctccagt ttccaggtca tatttctgta atttcttttc tgtcataata aaaataggca
     3721 taattggaaa tactagcaga agcatttcaa tcagaaaata attcgtatgc ccagatgtct
     3781 ttaaataata gccgatcaat gccatcagta agcccataag aaaaaaccag tttccgcttg
     3841 tcttttgagc cagccgccaa tgagcgtctg tttttgcggc aagaggcgaa tgatacccat
     3901 aaaaaggcag atttcctttt gagggcagaa tccgaaaaag actgccaatt attaagaata
     3961 agataccaat actgaaaaaa atcattcttt ccctcctaaa ttctcttctt atagtatact
     4021 ctaaattgtt gactgaacca atatgttcag gaattcttca cgaaaggttt gtcttttgtt
     4081 cattctttgt taagataaac ttattccaat aaaaaaggag ttttatttgt gtcaaattat
     4141 cgtgcaatca ctttctttga tcttgatgga acattactag atgcgaaatc aaaaatcaca
     4201 cccgaagtgg caaaagctat gaacgcatta aaagaaaaca atgttctccc cgtcatcgct
     4261 acaggacgta cagaagcaga aatccactct atcatggaag atgcgggcat tacttctgcg
     4321 gtcaccatga atggttcttt cattcgagta gacggcgaag aaatctattc tcaaacattt
     4381 tcttcagaag aatgtcaaaa aatgtatgat catgtacagt tgaacggtca tgaactttct
     4441 tactataacg atcagaaaat ctggtgtacc ggtcatagcc aaacaatgat caatgcttac
     4501 gacttcattc attcagatgt tccggaaatt gatcctttag gctttaaaga aaacacagta
     4561 aatatgttgc tgatcttatc tcaatcaggt gacgaatact atcacgaaca ttttcctgag
     4621 ctaacttttt atcggaatgg tccttactcg atcgatacag tgaagaaagg aatctcaaaa
     4681 ggggcaggtg tcaaacgtct gcaaaaagaa cttcagctgg aaaaagttcc tacttttgga
     4741 tttggcgatg gtccgaatga ttttgccttg ttggaagctt gcgacaacaa aatcgctatg
     4801 gacaacgcct atgacggatt aaaagaactt tctaccttta tcacaaagaa aaatacagaa
     4861 ggcggaattg tccacgcatt gaagtatttt gatctgattt aagatctttt tttgaagcta
     4921 cctcttttct taagaaaatc gcttattttt tcttaagaaa ggggcagtta ttatataaat
     4981 aatgggatat gattcgcttt tttattgaat ttatcccaat atatgctata ctaattttgt
     5041 atgaaaaata cacgtaaccc ttgttagcta acggggacgt tacggattcg acaggcacag
     5101 tcgagcttga attgcgtttc gtaggttacg tctacgtaaa aacgttacag ttaaatataa
     5161 ctgctaaaaa cgaaaacaac tcttacgctt tagctgccta aaaacagtta gcgtagatcc
     5221 tctcggcatc gcccatgtgc tcgagtaagg gtcctaactt tagtgggata cgtttcaact
     5281 ttccgtctgt aagttgaaaa agagaacatc agactagcga tacagaatgc ctgtcactcg
     5341 gcaagctgta aagtgaatcc ttaaatgagt tgactatgaa cgtagatttt taagtggcga
     5401 tgtgtttgga cgcgggttcg actcccgccg tctccatata tgtatggttg agacagataa
     5461 acatgtatga aaaacgttgt taaatcaacg tttttttatt ttggtcaagg agagaaaagt
     5521 agagaaatac aaaatttttt gcattgattt tgcattcaaa aaaacattca aacatttaga
     5581 aatccccaat acgtattcag gaaaatctat tttaaggttt acaaaaagaa cttttgttcg
     5641 tacactcttt ttgaggtgaa cttttatgct gatggaagga aaaacacaat tatggtttaa
     5701 gcatgttcct tcaaataagt tttttta
//
LOCUS       DAZUKC010000130         5700 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000130 DAZUKC010000000
VERSION     DAZUKC010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.135"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(246..749)
                     /locus_tag="ACG80T_002612"
     CDS             complement(246..749)
                     /locus_tag="ACG80T_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFS4842218.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1108..2133
                     /locus_tag="ACG80T_002613"
     CDS             1108..2133
                     /locus_tag="ACG80T_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS4842219.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2456..3334
                     /locus_tag="ACG80T_002614"
     CDS             2456..3334
                     /locus_tag="ACG80T_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFS4842220.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            3810..3926
                     /locus_tag="ACG80T_002615"
     CDS             3810..3926
                     /locus_tag="ACG80T_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFS4842221.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            4854..5192
                     /locus_tag="ACG80T_002616"
     CDS             4854..5192
                     /locus_tag="ACG80T_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842222.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5286..5516
                     /locus_tag="ACG80T_002617"
     CDS             5286..5516
                     /locus_tag="ACG80T_002617"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFS4842223.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT         1913 a          858 c         1050 g         1879 t
ORIGIN      
        1 tcctttctag attgagatat tgactaggaa gaatgttaag attttaagcc tttaagctaa
       61 aaaaaaaccc tttttgttaa ataaggggaa tatagcctgt cagtttcaaa ctgcaagtcg
      121 tcctgctgca ataatatagt agattagaga tgaaaataga tttttttaaa ccaatgactg
      181 aataaaaaga ccgtcataga tacacattgg aatgtgtcta ttacggtctt tttttacgaa
      241 cgattttatt gaagacggac agttcgggtc aaaaataaaa aagctacgat cagaaataag
      301 aataaaatgt atcccaaaat aatggctaga cggtctaact tatttgtcga tagatgtttg
      361 tttagaagtt gataggccca taaacctatc agtcctcctg ttgtattagt tatcaaatca
      421 gtgatgtccg ctacgccaat accgaatagg aattgaatca attctgccaa gaagcttaca
      481 aggaaaataa tccctaattt tttagaaaaa gaaagtctct tgaaattgac attcaaaagc
      541 accccaaaag gaaaaaagac aatcaagtta taagtggtct cacgtaatac cgtcgtattt
      601 tcaaaagaaa aagtagaaaa agggacgaga ttgacattgc tgtaagaata agtgagaaat
      661 tgtggaagat taaatgatag cttgaataaa atcaaccata atagaaagaa taaataaatc
      721 cctaataaaa attttgatac ataccgcata gacatctcct ccatttaaaa agtataccat
      781 cgaaaaccgc tgaaaaatca taaaaaagta tgaagtttaa ggaccaacgt agttatcgta
      841 cgaaaattag aagctttttt ttgttgggaa tccggtttct ttaacaaagc taagatagcg
      901 agttgcttgg aaaagttgct atcttttttc tgtcaaaacg actcggaatg ttcgtttttt
      961 gttctaattt ctccaagttg tttacttttt gatactaagt ttatgaaaaa tcaagaaata
     1021 gttgttatac taggtaatgc tgacaaacgg gattggcatt tcatcttggt tcagcttttt
     1081 ttccaattac taggaaggag ggatagattg ttcaagactt attttcgttt caataaaata
     1141 caagataatc cttttcgtat catgaatctg atgctactta tctttattgt attatggtta
     1201 ggttacataa atcatgattt tttgatttca agtttttcat cattagggat ctttactttt
     1261 ttctattatc aaaacatacc gatgaaatat ttgctgaaac gtatgtttct gatcggttgc
     1321 ggactgttgg ttgctttcgt tttggggatc ttatcgactt atgttttatg gcttgaacca
     1381 tttgccgtag ctttggttgc tttttccagc agattcgtgt tgcgattatt ccatatttct
     1441 aaaccaggtg gcttgttttt tgcgatgctt tctgccatgg gaacgagcat gcagctgcca
     1501 atcgctcaat taccgatcgt ctcactttat ttttttatgg gtgtcgtttt tgccctgatt
     1561 gcggcagtga ttacaaaatt actggattca cggcctgagc agacaattga aaaagcaact
     1621 ttaaaagaaa gatttcacga agagccttta gtgatcattg attctgtttt ctacagcgct
     1681 gccttatttc tttccgttta tgtcagtcac ggcttgaatc tgcataatcc ttattggttg
     1741 accttgtcat gtgcatcgat tttgctagca gaaaacttgg atgcgatgaa gcatcgacaa
     1801 gtccaatatc tgattggatc gatgggtggg ttatgtgtgt cggcattctt gtcatttgtg
     1861 ccgtttacgc agttgcagac catttttctg atcacttttc tatatggaat cgcacaattt
     1921 ctagttgcta gaaattatgc agttgctaat atttttctta atccgatggc gttgatgcta
     1981 tctacactta ttcgtggagc atacttgatt tctttgatcg aatatcgttt tttagggatc
     2041 gtcataggaa gtttcatcgg tttaggtgtt gcgtgggtaa tgacggttgg tttgcaacat
     2101 tatttaacgg ttgttcgtca aaaattagaa tagaaggaca aatatatgtg ccaaaagagg
     2161 ttatgacaag atttttgtca caacctctaa ttcaaggtgt tcaaaagcca gattctcagc
     2221 attaagtcaa attcacacaa aaatgtggaa aggaactttc aaaaaaattt tatatttatt
     2281 ctatcaaaaa attttgttta tgatggtaac tgaataaact ttcctgagat ggtcaatgct
     2341 ttccttgaaa gtcttacaga tattctttct tcatagaaag tttaagtttt atagttagtg
     2401 tacaataaaa gtatgtttat accatatggt gtccggataa tcagtcattt aaacaatgag
     2461 gttcaaagat atgaaaaggg gaaatttgat gaagaaaata agcaatgaaa tccaaattct
     2521 ggcagaaact ttaactcttc ccaaagtcgt tatagagcaa atgattgtac aagcaaaaaa
     2581 catccttgat gaggtgtttg agcaatatag tcatgaacta atggatccta ataaagcaaa
     2641 ggcgactgca aagaaaatag acaaagtcta tgaagaggaa ccttttgcag cattagctat
     2701 ttatctatat gcagcttctc aaagttggaa aaaaatttat caaccattga agattccaag
     2761 aactatctat ttggcaacaa tgaatgcttt tactagattt attcatgaac atttccaagt
     2821 aacaggaagc tatcgctttg accgtggttt ttggatttgg cattacacta gtggcttgat
     2881 ctttcggatt ggtgaattgg agtacgaaat gacatatttt ccgaaagggt acacggatct
     2941 aagattagaa ggaaagcagt gcctttctct tcatattcct tccgatgcag atttatctca
     3001 gaataagata agagatagtt acgaaagtgc cattcgtttt tttgaacttt attttcctga
     3061 ttaccattac caagtgatgt acacagatac ttggttacta tcacccaatc ttactaaatg
     3121 gttgaaaaaa gagtcaaaaa tctgtctttt tgcagccgat tatcgattat tatcagttga
     3181 tgaacaggac gatagtggag tcccttggat ctttggacga gtagatgccc aaatacatga
     3241 ttatccagaa agtacgagtt tacagcgaca agcaaaagag caattgcttg caggcgagca
     3301 cattggctct ggcttaggta ttcttacttc ataagtttag tacttcgaaa tagtttaaaa
     3361 gagcgtgaga caaaagtaaa gaacactttt gtctcacgct ctaaacacgt ataaacggcg
     3421 gaagcagaag caactccttc ggaaataagc tgaaattcac aaaaatttga aaagcaattt
     3481 tcgtgaattt cttcttgttt cttggagtta aacgcttctg tcccagcctc ttttttagtg
     3541 ttctatcgaa cacacagtac gttcaatagg taaactagct ataacaatag atattaaagt
     3601 taacggacaa gagggccttt tttaggtagc taaaacgtaa taatgaaaat ttatttccag
     3661 agataggagt caatcagcta gtctgatcga ctgttttaac aagttgactt tcattttaac
     3721 ttttgatacc atttaaaaca caaatcgtaa ttgtttcgat ttgtgttttt tagcatgact
     3781 acaatcaaag aagaaagtgg gtcgtccata tggaaaaaac agatatctca agtgcatacc
     3841 gccgcttgaa aagcccaaat atcaaaacac gaaaacgtgc gctgaagatc atcaaagatg
     3901 tgaaacggaa tagtgggaaa aggtaagaag aaggaaactt aattaaaaaa atagccgaaa
     3961 aacgtttttc ggctattttc aataatcggt ttttgtgtaa ggtttccgaa agaactgctg
     4021 ttgattttcc atttttaaca tatgataaat tggtagaatc tttcttttca aattgatacc
     4081 attccctaat tccagttata tagttaacta cagaaccaca aagcacgtac gtttataaaa
     4141 gtattatctg tagttatttt tcaattccct ttcaacctct tattttgacc agtagtattg
     4201 atcactttac cttttaacac ctttgttcgt ttttgaaaca tttttagtca agaatggaaa
     4261 cgtaactaca taatcaaagg ttttgcgaaa tatttcatga aatatttcat gtagaagcga
     4321 gcaatacggt tgcaaataac cattatcgtt taataagcag ttacgaaagc tcccaatatc
     4381 tctaacgttc agctcactcc atttcttttc ttcaaaaaaa tcagtgattc gtatctcttt
     4441 ctatttgtcg aaaaagttaa ctttaaaaag taacgccgaa aaaatatgca aaaaaataaa
     4501 aaagcagtta ctattgctag aataacatca aggaataata agttttggtt ttcagataat
     4561 agtcgataag ttgtgtattg gaattttaga tacagtaata aagtattttt catgaaattt
     4621 tgtgagaatc actattacaa gtatctttaa taaaattaat ggttaaggag gaacttaaag
     4681 tagaatgtaa atgaatagtt gtagtcagtc gattgtgata ctagactcaa agttgcagga
     4741 ataagttttt caatttgtga ttggaagata aaaaatgaaa aagaagggct tttttttata
     4801 ctttgttgat tgtagtgttt attttccctc tgtttagcca agtaacattt tctatgactc
     4861 aaccagtttc aagtgaggaa catgataacc tttttcgtgc tacagaagag tttaagaaat
     4921 atcaggaaaa tattgttccg gagactccat tagctagtga gccaattact gatattgatg
     4981 gaaaccaaat aggatggtat atacagtacg aagttatggt ggtaaataat gactcaatag
     5041 taaaaaatac agtaggctta gagctaacac cgattgaaaa ggtgagttca ttgttaacgt
     5101 atatttatac tgatgaaagt gacgagactg agatcatttt aacggattac tcaaaaattg
     5161 ctactgataa aaagtatata ttgtggatgt agacactagt gaaatacttt atgatataga
     5221 aattgatgaa aatttactag atgtagcaca agaaatcgat catcaaaagc aggaagctat
     5281 tcgatatgct ggtagaaaag ggaaaagaaa atatttatta cgtgaatgta gcaaaagtta
     5341 gagaagatga aaatgagtgg aaagaattca agagtcgcta ctcaattaac tcgactccga
     5401 catttacagt ttacagagaa ggatcgatag aaaaaactgt tttttggact aaagaaagtg
     5461 gaatgtcttt agctgaagta gaagagtttt tggattatgt tagtatgcaa cagtaaggga
     5521 gttcttatta tgaaaaattt tgacacatat tatgttattg ttttttcgat atctttgctg
     5581 ttaatattac taactttaat gcagtataaa gagtatccag agtattttat tttaactttt
     5641 gtatatttag taagtagcta cacacttcta tataaattca gaagttcttc tagagtttct
//
LOCUS       DAZUKC010000131         5498 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000131 DAZUKC010000000
VERSION     DAZUKC010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.137"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            238..1629
                     /locus_tag="ACG80T_002618"
     CDS             238..1629
                     /locus_tag="ACG80T_002618"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFS4842224.1"
                     /translation="MNKNGEWNAARRIFIILMDVLVLNVSIYLSFLLKFQGEVPVRNL
                     ETFQHSAIFISIIFIALNILLGAYVFYNRMISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVILISFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVAIDNYYENTKKLIDDMDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENISS
                     LAEIDFSQEKIYR"
     gene            1688..2491
                     /locus_tag="ACG80T_002619"
     CDS             1688..2491
                     /locus_tag="ACG80T_002619"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4842225.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGKRIVPEYNNAIRVFSKRRNPFNHMTVMFD
                     KKAILEVGNYQPLSGFEDYYLWARLLKAGYKGYNIQETLVYARTGKDMYARRGGMKYL
                     GSGLMGRYRIWKDGLGSVKDFIIVSCGHIFISLLPNQLRGKFYQTQLRK"
     gene            2524..3378
                     /locus_tag="ACG80T_002620"
     CDS             2524..3378
                     /locus_tag="ACG80T_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HFS4842226.1"
                     /translation="MKKIQQITLKMSQEIVDFCLKNQLLCYFCGGGAIGTVREQGFIP
                     WDDDLDFFMPREDYEKFYQLWVNSEKSKKYPIQKASKNYNDHNSFTTIRDADTTFIKT
                     YQRDLDIVHGITIDIFPLDVAPVSKFSRKIQKLWALVYALFCSQVVPEKHGGIMATGS
                     KILLSIFRSPRTRYKIWSFAEKQMTKYNNEPTGYVTELCVGPKYMGNVYPAKDFESAV
                     WVPFEDTEMPVPVGYDNYLSQVFGDYMQLPPEKDQVSHHEAVYIDPEHPYTMYKGKYY
                     LTKGAEKK"
     gene            3375..4076
                     /locus_tag="ACG80T_002621"
     CDS             3375..4076
                     /locus_tag="ACG80T_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFS4842227.1"
                     /translation="MITAIIIAGGVGKRMGQDIPKQFITIEGKPIIIYTLESFQHHPQ
                     VDRILVVCKSGWEETMWAYIKEFNISKVKWVITGGSKGQESINNGVQFLKDYSEDDDT
                     IIIHDGIRPLVDELVLSDVIVKCKEFGNGVTSLPYNEQIFIKKTEETTEQYVDRNTLR
                     RVSTPQAYQYGKLRAAYDRAVKEDIGMTDSSYTNTMMVDLGETLYFAAGSDKNIKLTT
                     TDDLELFKAYLKMKD"
     gene            4084..5151
                     /locus_tag="ACG80T_002622"
     CDS             4084..5151
                     /locus_tag="ACG80T_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFS4842228.1"
                     /translation="MMTLIKQPMYQEDLAKVINHTPKIKQLHGKSVLMIGASGMIGSF
                     LIDTLMAANEQLGIKIKVYAMGRNRQKLEKRFASYLELDTFEIVEGDVTEPLPKEIQA
                     DYLIHGASNTHPKAYATDPIGTIMTNLAGTEQVLKHAVATQPVRVLFLSTVEIYGENR
                     GDIEKFTEDYCGYIDCNTLRAGYPEGKRVSESLCQAYIAKHDLDIVIPRLCRTFGPTM
                     LLSDTKASSQFILNAVEQKDIVLKSEGNQYFSYVYVGDAVSAILHLLLEGEKGEAYNV
                     SSENFDLRLKELAQQLADISGKQVIFELPDEIEQKGFSKASTAVLENKKIKETGWKPI
                     FPLNESLVHTVELVKGEKYNV"
     gene            5144..>5498
                     /locus_tag="ACG80T_002623"
     CDS             5144..>5498
                     /locus_tag="ACG80T_002623"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4842229.1"
                     /translation="MCEISIIVPVYKVEKYLRKCVDSILAQTFTDFEVILVDDGSPDN
                     SGKICDEYAEKDNRVRVIHKENGGLSSARNAGIDVARGKYLGFVDSDDYIDEDMYEIL
                     YENLKIHDADISSVEL"
BASE COUNT         1927 a          825 c         1072 g         1674 t
ORIGIN      
        1 tatatgaata ttgtaacttg tgaataagga attagcagga gaatactagt tcagcataaa
       61 ggaagtgatg ggacttctga gacaactttc tgttatgcag aaatacacat ataaacagac
      121 aataaacgct tttccccgtt tcttttaatt tcctttacaa tttggttatt cactaggttt
      181 ttaaactttg ctatggtaag atgatagagg cattatttta tggttaggag tgtatatatg
      241 aataaaaatg gggagtggaa tgcggcacgc agaatattca ttattcttat ggatgtactt
      301 gtattaaatg tatcgatcta tcttagtttt ttgctgaaat tccagggaga agtaccagtg
      361 agaaacttag aaacctttca gcattcagca atttttattt ccatcatctt tattgcgttg
      421 aatattttgt taggagctta tgtattctat aaccgaatga tcagtgatat tgtcttcatt
      481 actgttattg gacaagtact aatagcattg gggatcatgg tagttacttt tgctggccga
      541 tggtttgcat ttccgcgtgg cgtcattttg atctcgttta tcctaggtac gatattactt
      601 agtgtttacc ggatagtcgt ttacaaactg tacttaagag tcagtcgtga taagaaagtc
      661 cttatcgtgg gtttggagaa agatgtcgca ccagctatcc aaaattttca atctaagaaa
      721 aacaataaac ataaggttga agcagtggca atcgataatt attacgaaaa tacaaaaaaa
      781 ttgatcgatg atatggatat cgtctatttg gcttctaaaa tcccagaaga tgaaaaaatc
      841 aacatttatc aattagtgac taaaaaagag aagaaattat ttttaaatac gacttttgag
      901 aatctgacaa tgataaatcc gaatattatg aattttgaag atgaaagcat cattgaagct
      961 tctgggttca gaatccaacc agaatacgaa ctattcaagc gtctctttga tatcatcgtt
     1021 tcgcttattt tactgatcct cgcctcacct tttatgctaa tcacagcgat tttggtaaaa
     1081 acaacttcac ctggaccaat catctataaa caagtacgta ttacgaaaaa tcaaaaggaa
     1141 ttttcaatct ataaattccg ttccatgagt gcgacagctg aagcaaaatc aggaccagtc
     1201 ttggctaaaa gtaacgatgc acgtgtgaca ccagtaggta aatttatccg tgctgtacgt
     1261 tttgacgaat tacctcaaat ctttaatgta ttgaaaggcg atatgtcgat tgtcggtcca
     1321 aggccagaaa gaccattttt cgttgatcag ttcaatgaag aaaatcctta ttattactta
     1381 agacacaatg tacgtgcggg aattaccgga tatgcacaag tatacggaaa atatgtatca
     1441 gattataaca gtaaactacg ttttgatttg ttgtatatca aaaaatattc attactatta
     1501 gatttgaaga ttttacttca aacaatcaaa atcttatttg ataagatgtc atctcaagga
     1561 ctagatgaag atgaaaatat tagttcatta gcagaaatcg acttttcaca agagaaaatt
     1621 tatcgctaga ttggaaaaat tttgagcaaa gaggtaggag agaacattgg aaaataaaaa
     1681 tgtaagtatg ctgctttctt tatatgataa agaaaagcca gagtatttaa aggaagcatt
     1741 aactagtatt tttgagcaaa cagtaatgcc aaaagaaatt gtattagttt atgacggccc
     1801 tataaatgaa aaacttcaag tagttgtcac ttatttccaa caaaaagatc ctcacttgtt
     1861 tacagttgta aaattagaaa aaaatcaagg attggggatt gctttagcaa ttggattaga
     1921 acatacgaaa aatcgcttag tagccagaat ggatacagat gatataatgg aaccccaacg
     1981 attagaaaaa caattagaga tcttccaaaa agatccagaa atagcgattg ttgggagtaa
     2041 tattgaggaa tttactggca cgcttagcaa tgttatcgga aaaagaattg tacctgaata
     2101 taacaatgca attcgtgtat tttctaaaag aagaaatccc tttaatcata tgaccgttat
     2161 gtttgataaa aaagctattt tggaagtggg aaattaccaa ccattaagtg ggtttgaaga
     2221 ttattatctt tgggcaagat tattaaaggc aggttataaa ggatataata ttcaagaaac
     2281 attagtctat gcgcgaactg gcaaagatat gtatgcaaga agaggcggaa tgaaatatct
     2341 tggttctggt cttatgggtc gttatcgtat ctggaaagac ggtttagggt cagtaaaaga
     2401 ttttataatt gtttcttgcg gacatatttt tattagctta ctaccaaatc aattaagagg
     2461 aaaattttat caaacgcaat taagaaaata ggaggttttt ctttgtatag cactgatcca
     2521 aaaatgaaaa aaatacaaca gattacgttg aaaatgtctc aagagattgt tgatttttgt
     2581 ctaaaaaatc aacttctttg ttatttctgc ggtggtggtg ccatcggtac tgtgagagaa
     2641 caagggttta ttccatggga tgatgattta gatttcttta tgcctagaga ggattatgaa
     2701 aaattttatc aactatgggt taattcagaa aaaagtaaaa aatatcccat acagaaggct
     2761 tctaaaaatt ataatgatca taattctttt acaactatcc gcgatgcaga tactacattt
     2821 attaaaactt atcaaagaga tttagatata gtccatggaa ttactattga tattttcccg
     2881 ttagatgttg cccctgtttc gaaatttagc cgtaaaattc aaaaactatg ggctttagta
     2941 tatgctttat tttgttctca agtcgtccct gaaaaacatg gtggaataat ggctacaggt
     3001 agtaagatac tcttatctat tttccgctca cctcgtacga gatataagat atggtctttt
     3061 gctgaaaaac aaatgacaaa atataataat gaaccaacgg gctatgtgac tgaactttgt
     3121 gtcggaccta aatacatggg aaatgtttat cctgcaaaag actttgaatc tgctgtgtgg
     3181 gtgccattcg aagatacaga gatgccagta ccagttggtt atgataatta tttaagtcaa
     3241 gtttttggtg actatatgca gcttccacct gaaaaagatc aggtttctca tcatgaagca
     3301 gtttatattg atccagaaca tccatacaca atgtacaaag gaaaatatta tttaactaaa
     3361 ggagcagaaa aaaaatgatt acagctatta ttattgctgg tggtgtgggt aaacgaatgg
     3421 gacaagatat cccaaaacag ttcattacaa ttgaaggaaa acctattatt atctatactt
     3481 tagaatcatt tcaacaccat cctcaagttg accggatttt agtcgtttgc aaatctggtt
     3541 gggaagaaac gatgtgggcg tatatcaaag aattcaatat tagcaaagtc aaatgggtca
     3601 ttacaggtgg atcaaaggga caagagtcga tcaataatgg tgttcagttt ttgaaagact
     3661 attctgaaga tgatgatacg atcatcatcc atgatggaat ccgtccacta gtagacgaac
     3721 tggtattgtc agatgtaatc gttaaatgta aagaatttgg taacggtgta acctctctac
     3781 cttacaacga acaaattttt atcaagaaaa cagaagaaac gacggaacag tatgtcgatc
     3841 gtaacacatt gaggcgtgtc tcaactccac aagcttatca atacggaaaa ctacgggcag
     3901 catatgaccg ggcagtgaaa gaagacatcg gtatgactga ttcttcttat acgaatacca
     3961 tgatggtaga tttgggagaa actttatatt ttgctgctgg ttctgataaa aatatcaaat
     4021 tgacaacaac agatgatcta gaattgttca aagcatattt aaaaatgaaa gactaggtga
     4081 aagatgatga cgttgattaa acaaccaatg taccaagaag atttggcaaa agtaatcaat
     4141 catacaccaa aaataaaaca gctacatgga aaatccgttc tgatgattgg tgcttctgga
     4201 atgatcggct cttttttgat tgatacgttg atggctgcta atgaacaact aggcatcaaa
     4261 atcaaagttt atgcaatggg cagaaaccgt cagaagcttg aaaaaaggtt tgccagttat
     4321 cttgaattag atactttcga aattgttgaa ggtgacgtca cagaaccctt accaaaagaa
     4381 attcaagctg actatttgat acacggtgca agtaacacac atcctaaggc ttacgcaact
     4441 gatccaatcg gtacgatcat gactaatctt gcaggtacag agcaagtatt gaaacatgca
     4501 gtagcaaccc aaccggttag agtattattt ttatcaacag tagaaatcta tggcgaaaat
     4561 cgaggcgata tagaaaaatt cacagaagat tactgtggat acatcgattg taatacactg
     4621 agagcaggct accctgaagg aaaaagagtc agtgaatcat tatgccaagc ctacatcgca
     4681 aaacacgacc tcgacatcgt gattccaagg ctttgccgta cgtttggtcc gacgatgctt
     4741 ttatctgata cgaaagcatc ctcacaattt attttaaatg cagtcgaaca aaaagatatt
     4801 gttttaaaaa gtgaaggaaa ccaatatttt tcgtatgtat atgttggaga tgctgtttct
     4861 gctattcttc atttactgct tgaaggagaa aaaggagagg cgtacaacgt ttcttcagaa
     4921 aactttgatt tacgactaaa agaattagcc caacagctag ctgatatcag cggcaaacaa
     4981 gtaatttttg aattgccgga tgagattgaa caaaaaggtt tctcaaaagc tagcacagca
     5041 gtacttgaaa ataaaaaaat taaagagaca ggttggaaac caatttttcc tttgaacgaa
     5101 tcattggttc atacagtaga attagttaaa ggtgagaaat ataatgtgtg agattagtat
     5161 tattgttcct gtgtataaag ttgaaaaata tttaagaaaa tgtgtagatt cgattttagc
     5221 ccaaacattt actgattttg aagttatatt agtagatgat ggatctcctg acaatagcgg
     5281 gaaaatctgt gatgagtatg ctgaaaaaga taatcgagta cgtgtgatcc ataaagaaaa
     5341 cggcggatta agcagtgcta gaaatgcggg gattgatgtt gcgagaggca aatacttagg
     5401 ctttgttgac agtgatgatt atattgatga agatatgtat gaaattttgt atgagaattt
     5461 gaaaattcat gatgccgaca ttagctctgt tgaattga
//
LOCUS       DAZUKC010000132         5377 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000132 DAZUKC010000000
VERSION     DAZUKC010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.138"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            236..1111
                     /locus_tag="ACG80T_002624"
     CDS             236..1111
                     /locus_tag="ACG80T_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS4842230.1"
                     /translation="MKESASCFVLSYSGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAIEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            1215..1979
                     /locus_tag="ACG80T_002625"
     CDS             1215..1979
                     /locus_tag="ACG80T_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFS4842231.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            1966..3408
                     /locus_tag="ACG80T_002626"
     CDS             1966..3408
                     /locus_tag="ACG80T_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4842232.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAIFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            3419..4749
                     /locus_tag="ACG80T_002627"
                     /pseudo
     CDS             3419..4749
                     /locus_tag="ACG80T_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
     gene            4762..5220
                     /locus_tag="ACG80T_002628"
     CDS             4762..5220
                     /locus_tag="ACG80T_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFS4842233.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
BASE COUNT         1819 a          778 c         1121 g         1659 t
ORIGIN      
        1 tttcttatag agagttgcta tatttttttt gtttttatgt aattacctcc aaatcaattc
       61 gatacaagta ttatcataaa acgagtaaaa aagatattga taacgctttt attatgtgtt
      121 aaactatttt tgtaaattat gtagggtgct actattcaat taggcacgac ctaagaaagg
      181 gttctttctt aggtcgtttt ttgtctttaa aaattgtttg gagacaaaaa tatgtatgaa
      241 ggagtctgca agttgttttg ttttatcata tagtggaggt gaattaacgg tgtatgtgat
      301 gaaaaagctg aatcataatg ctgtattgat cgatgacgat ggaatagaaa aaattgcgat
      361 ggggaaaggg attggctttt ctgctaaagt gagtaagcca tttgatgttg aggatgctga
      421 caaattattt gtattagatt ctaaagaaaa gatcaaacga ttcagtgaga tagtctcgca
      481 gattccaatc gaatttatgg attttgcgga agaagtgatt gaactgataa cagctaggat
      541 ctcctctcca ttagattcaa atatctatat tacattgaca gatcatattt attttgctat
      601 tcaacgaaaa agagaagata gagatgtcac agcaattatg ttgccagaaa tgagattcct
      661 ttatccaaat gaatatgata tagcaataga agtggtcgag cgaatcaatc atcgatatca
      721 aaccgagtta gggtcaaatg aagtaggatt catcactatg catatcatta atgctgagct
      781 aggagagcct aatagtttaa agagcttaaa aatagtagaa atgacgacca cgattttgtc
      841 ccatctagaa aaatcctatt caaacgttta tgataaagaa tcactgactt atcatcgctt
      901 aatggttcat atcaaattcc tggttaaacg cttgatttat caggaagaaa cgccagtaga
      961 aaatattggt ttttttactg aaacatttaa acatagcaaa gcgtattgta cagcactaaa
     1021 gattaaagat atcatcgaac agcaatttca acttgccatt caagaaaatg aaatcatcta
     1081 tttggcaatt catctagcac gaatctatta aaataagggc gtaactatct ataggcgcaa
     1141 ccaaatgaca catagtaaat ggactatgat tatttggttg cgcctatttt agtagaaaaa
     1201 agaaggagaa tgaaatggga aaagtgattt ttaattcgga tgattttggc tatagccatg
     1261 gtgtgaatta tgggattatg gatgcttatc agcgaggaat tttgacatca actacattga
     1321 tggcaaatat gccgggtttc gagcacgctg tgaaactaag aaaggaaatg cctaggctag
     1381 gtgtaggagt ccatttaaca ttgacgtgtg gcaaaccact tctcaaaact gtcgatactc
     1441 ttatcgaaag cggagagttc agatccttat cttattatca gcatccattt aacattgaca
     1501 aagatcaact gtatcaggaa tggaatgctc agattcaaaa aatttatcat gcggggatca
     1561 taccaactca tttagacagt catcatcata ctcatacttt ggatgaaaac caggaggtag
     1621 ttgttacatt agcaaaaaaa tatgatttgc cggttcggag caactttgaa cgaaaagatg
     1681 aagttcgtca tgtcgattat tttgagcctt gtttcgatgt cgtaggagaa actgatgaaa
     1741 aaaaacgaca aatcaatacg agtgtggaag aatatttggc aagtttactc atgatattga
     1801 gagaagataa aacgatcgaa gtcatgtgtc acgcagcgta tttggatcag gaattgttaa
     1861 atggttcaag ttttgtgtat cctcgtactc accaagtcga atttttaatt cattcagcat
     1921 ttgctcaaaa agtgaaaaat gatcctttga ttgagttagt aacatatgag gatatttaat
     1981 agaggaggaa taaaaatgaa tgttaaatca aatgtgcaga agcttggaag agcgatgcta
     2041 cttccagttg cagcgatgcc attagcggga ttgatcatgc gtttatcagc tgatgatatg
     2101 ctgaacatcc cagtaattgg tgcagctgga aatgccgttt ttggaaactt ggatatctta
     2161 tttgctatag gagttgcaat cggttttgtc aaaaataagg acaaaggaat acctgcactt
     2221 actggttttt tagcgattgc taccttgaaa aaaggcttag aaatcatgga cccagaagtg
     2281 aatatgggga tttttggtgg aattatttcc ggtttagtag cggcatggac gtataatcgc
     2341 tttaaagaac aaaaattacc agtagttttt tcttattttg cgggcgaaaa attcccattg
     2401 actatggtga tgattctaca aacaatcgca gctatttttt tgggctttat ctggccgctc
     2461 atccaaattg ggattgataa ttttgcaaaa ctgctcgttg atatgggggc attaggtgtg
     2521 gggatattta tgtttttgaa tcgtttgctg atcccctttg gactgcacca tgtgttgaat
     2581 acgtatattt attacgattt aggaagctat acatctccta gcaaggaagt tttcagagga
     2641 gagatgacac gctttatcaa cggagatcct caggctggac tgtttttatc aggttttttt
     2701 gtagtaatga tgtttggtat accagctata tgtctagcta tctataaagc agcttataaa
     2761 gaaaataaag agatggtcaa aggaatcatg ggaagcggtg caggaacttc atttattgca
     2821 aatatcactg agcctgttga atttagtttc atgtttcttt ctcctatgtt gtacgtgatc
     2881 catgcacttt ttgccggttt agcagggatt gtctgttact tattcaatat acgaattggt
     2941 tttacattcg gagcatgtat tgttgattat ctcatcaatt ttcgaatcgc taccaatgct
     3001 attttgattc ttccaatcgg aattttcttt tttgccttgt attacgttac tttttattat
     3061 ttgatcaata agagaaacat tcagacatta ggacgtgaag ctaaggcaga gtttggcaat
     3121 gaagtaacct tagaagaaac agagttaggg ttagcaagta aaaattatga atatatggca
     3181 acaaaaatgc tacaagcgtt tggaggaaaa gccaatatcc tggatgtcta tagttgtaat
     3241 acacgcctac gggtagaggt agtggatccc accatggtcg aggaacagcg aatcaagcaa
     3301 ttagggataa gcgggatcat taaaccaact gaaaaaaatt atcaaattat catcggctta
     3361 gaagtgactt atgttatggc agaattcaat aaattattag aagaatagaa aggaaaagat
     3421 gatgatgaaa aaacagatta tcactatagc tggaggcggg agtacgtata caccaggaat
     3481 cgttcaagcc attttgaaca atgaaaaacg gttgccatta tctgaaatac gtttatatga
     3541 tattgatgaa gaaagaaata tggatatgta cttgattgtt aaatttatgc tgaaaaaaaa
     3601 ggtttttcaa atattcgcat tcgtgcaacg gatgatccaa agcttgcttt tacaggatgc
     3661 gattttgttt tctcacaaat tcgtgttgga ggattggaga tgcgagaaaa ggacgaaaag
     3721 attccactta aacatggatt agtaggacag gaaacgtgtg gactcggagg atttgcttat
     3781 gggatgcgct caatcaaagg tttattggaa attgtcgatc atatacaaga ctatgccccc
     3841 aaagcatgga ttttgaatta tacaaatcca gagtcgatcg tttctgaagc tgttcgtcgt
     3901 aaatatccga atgtaaaaat gattaatgcc tgtgatatga ctatttcaat tgaagaaacg
     3961 attgcagtga attacggtta tgatcgtaag aattggatcg ttacgtatta tggtttaaac
     4021 cactttggct ggtatacatc gatttatgat aaagaattgc aacgagaaat tatgccagag
     4081 attattgaaa aactaataac aaaagaaatg caagtcgccg attttaatat aggagacaaa
     4141 acttggcaaa aaacctttca aatgatgtca gtcatcacga aaaattttcc aagtaatatc
     4201 ccgaataatt acttagagta ttacttatat cctgatatgg tcgttgagca tactgacaaa
     4261 gagtatacac gtgctaatat ggtcatggat ggacgagaga aaaataccaa agaaatggct
     4321 gataaaatac gccgcggtat tgtggaagaa gtattgaact ttaattttgg ggagcatgga
     4381 caatatatcg tcgatatagc tatttccttg ttgaatgatg atcgtcgtcg ctttatgcta
     4441 atcgttccta atcaaggggc gattccgaat ctacggtcag atgcagttgt tgaaataccg
     4501 gcatatgttg gtgcaactgg tgtggagaca attacactaa gaaaaccaat cagtgatttt
     4561 cataaaggat tgatggaggc acaagttgca gcagagaagt tactagtaga tgcttacttt
     4621 gaaggttctt atcaaaaagc tttacaagca tttacattga atcaaaccgt accaaatgcc
     4681 agagtcgcaa aaaaaatact agatgaaatg atcaaagtaa acaaaaaata ttggccagca
     4741 ttaaagtaga ggtgataaaa agtgcatttc tttaaaacaa gaaacattct aaaagctgta
     4801 gctagtggtc aaattattcc attaacagca gtaaaagacg aggtcttttc ttcaaaaatg
     4861 ataggagttg gatacgctat aacgaatcat gatggatata tccatgcgcc agttaaagga
     4921 ataatcgata atatatttcc tacccgacat gccataacga tcgaaagtga atctggcaga
     4981 aaaatactga tccatatggg tttagataca gttgaactcg aaggaagtcc gtttcagata
     5041 cgggttgaaa gtaaccagga ggtaaaagaa gggacactgt tagctattat agatttgaag
     5101 aagttggagc aagcacaaaa agatacggtg atcattgtag tttctccagg catttttgaa
     5161 ggaaatttat taaaacaaaa ccaagaagta aagattaaag atagttgttt tgaattttag
     5221 tgtacgatga aaaaagttaa atagggtgat atttatgggt gaatttacaa aatttttttg
     5281 aaatgataag tttcaatcac tagatttaat cattttgatt tttgttaatt atcgaatttt
     5341 tttgttgctc ctatgattta gaaaacttat tttcagg
//
LOCUS       DAZUKC010000133         5245 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000133 DAZUKC010000000
VERSION     DAZUKC010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.139"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1273..1938)
                     /locus_tag="ACG80T_002629"
     CDS             complement(1273..1938)
                     /locus_tag="ACG80T_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4842234.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2064..4100
                     /locus_tag="ACG80T_002630"
     CDS             2064..4100
                     /locus_tag="ACG80T_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFS4842235.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4104..4742
                     /locus_tag="ACG80T_002631"
     CDS             4104..4742
                     /locus_tag="ACG80T_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4842236.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4770..5198
                     /locus_tag="ACG80T_002632"
     CDS             4770..5198
                     /locus_tag="ACG80T_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842237.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
BASE COUNT         1730 a          803 c          958 g         1754 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgaggacta cctgtattag tcctcttttt
       61 tgaccagatt ttgaccagtc tattttacat tctaatatat tcatatgagc taattttatg
      121 attaaatggt aagtgtttcc ttgtttgttt tagtttaaac tagtgtgtaa tttaaaaatc
      181 tatatggaag ggtctgtggg gtaatactta aataaaggct aggaacctga taacatcaag
      241 gttcctagcc tttttataat aaaatgaagc gaaaataatt tcttttaaag agccgagatt
      301 atcagctata taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg
      361 tagctctgct tatatgtttc gaatttaaga caaagagttg gcaaaagatg ttgataagga
      421 agataagata actgtgttat ataaactagg taactaactg taacaagcta attttaagag
      481 attcttatta gaagacgttt aggaatataa ctaagaaaat gattatacag gtaattgtca
      541 tttcaataat gttttctaat tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg
      601 gaaaaaagtt cgtacgatag tgaagagggg caaaatttcc tactaatatt cccactaaca
      661 agagataaaa cggccaggtt ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa
      721 tcccatagat taacaactga acaattttta caatgaaaaa attgttctgc cattactcaa
      781 agtctcttcc tccttgtatc gtttatttgt ttcattttat cattatgata ggcgcttgaa
      841 caatccattt tgtattttat tgagtactct tggataaact accagcgatt atcatattta
      901 tttatgttac tgtaagccat catataaaag taaatgagca gatcatagaa atataaaaaa
      961 gaactaaaat tgatctaaat tatgtgcagc ttgactacgt accaatctat attacttact
     1021 agattggtcg ttaatctatt cggatcgaat gagcaaaata tttttgtgaa tgtccatcct
     1081 tgattatgat tccaaaaaga atcattggga ggctgtggcg taaatattat actataagcc
     1141 agaaatacct atcattctga acttttttat ttaggaactt tcatctcaag gctttttcaa
     1201 ttaatttgta cccctgtttt ttggataaga tcatgtgcat tgattttttc tatctctacg
     1261 ttcatctcct acttaatgcc tatctggctc ctgagggctt ctttttaagg cgtcgtcata
     1321 gtcttggctg aggatttctt ggtaatctga gggaatggct tcccaagtga gcaggcgaac
     1381 gttcatgacg tgttttcctt tgtgtttgat tacagcatgg tttttctctt tttctacctc
     1441 tccagcgtga aaagacagcc atgtaccatg gccaaagata tcattacata cccaacgttc
     1501 gatggaattg tccacgtatc ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa
     1561 aggattgaat cgatcgatcg tttcattttt gtattgataa ttgataagaa aaagatagac
     1621 caaacaaaag acgagcagaa agaaagatag tgtctttttg agtgagaagg gttgttttga
     1681 tgaataagat ttcttgttaa caagaggttc atctggcaat gttggttcta ttgattcggt
     1741 agctgattca atagaaggaa aatcaatcgg cagacctaaa gtatgatgta aaagtgacag
     1801 catttcaagg tctggcattg tacgagaatt ttcccaacta gaaatcgttt tatcggaaac
     1861 accaattttt tcgcctaatt ctttttgcgt aagcttacaa tttctgcggt aagttcgtat
     1921 tttttctccg gtgttcatct tcttctcctt tcgattctac agtatataga tttattctac
     1981 atgacctata cacttgatag atacgttttt ttgatgaaaa acggtatgat tttatcaaag
     2041 aatcatttaa gtaagggaga gtggtgataa tcaaaaaaaa gttcacagtt ttactcacaa
     2101 tcatagtgac cggtatcatg agtggttttg ccttggttag tataaataaa caggtactct
     2161 atcggacaaa tgaatttttc catcaaacag atacttatac tccgattcga ttgccacaac
     2221 attttattgg gcaagaagaa tatgagcagc taatcatggt atttgaaaat gtctcatcag
     2281 aaactgagct ttcttatatg aaaaaaacag taaatgaatc caatgaagta gatacatctt
     2341 ggaacttcaa gatacagcca aagcttgaaa tgacattgta ttatcaaggg gatgtcaaat
     2401 cttctcacga gaaggtaccc tattctaata cctcctttcg cgtaacgtcc attgaagaat
     2461 cgatcaaaat aaaagatttt gaaggtgaat ttttcttgat gacgagagaa aaagaagcct
     2521 atgaacggtt catttcacta ttcactgatc gattcaatca agcatttcac actgattatg
     2581 ccactaatat ttttgaagac tttgaaaata gctattcgta ttcggttatt tcaaatacag
     2641 acgtatataa ttatcgagtg tatttaacta ttggattgat tcttttattc cttttgttat
     2701 catccttttt cttattgatg aataaagaaa tcagtttatt gttgaataat gacttttcat
     2761 tacatcaagt attgtggtgt ttattgggga aaaagaaaat cattattaca ttcataattg
     2821 caggaatcat tggtggtttt atttttatgt attataagga gcaatttctt tatttgttcc
     2881 agatgtacgt tttcttgttc agcggaatat atggtatctc tttgatttct cttttattag
     2941 ttgaaaaaat cgcttcgtta agagaagcgt tcaactatat ccaatacatt ctttttacat
     3001 ttggattgtt gttttctctt cttttgaccg ttgccaatgt cgaattagca actatcgttt
     3061 tttcttcttc acagttagct gtttcccatg aaccaccaaa agaaatcgct ggtgaggact
     3121 atcatgtatt ttatccagta accatcggga aaaaccacgt ggattttatt tattccaatc
     3181 gttttgcatc agcagcagat caggaattat atgaaacttt gaataaaaac ggaagtatat
     3241 tagtcaatgt atcagattac ttgaatgaag aaaatgaaca gtttggcaga gggataaaaa
     3301 taaatctgaa ttatcttaag aaatttcctt tgattgatgt ctctggaagg ttgatccaaa
     3361 taaccgaaaa agaaacgcac cctgttatat tgatccctga acgatataga catacagatt
     3421 taaaaaatga cctggcgcaa ataacagaat attatcaaga aatcagtgaa aaagaaccga
     3481 aatttctatt tataaaaaac aatcaaccca tttatacatt tattccttct atcccctgga
     3541 tcgaacatta tccggttgtt gaaatactga cattgaaaaa cagtatgtat ttggaaagaa
     3601 atattctcag cggagaaatc tatccaccgt taaagatcaa gataggctct ttgtcgaaag
     3661 aaaggttatt tgagctgatt gaagaaagtc agttacgtga taacttgcag ttatcatttc
     3721 cgtatactca gactgaagag atagctgtca aagtgttaag cagatcattt cattacttga
     3781 tacagatatt tttactcact ttcttttctt tcttcttgtt gagctatgtc atgttatgca
     3841 tatattttgt ctacaattgt cggaaaatcg ctatctttcg ttcaagtggt tattcgctct
     3901 ttgaaacgta taaagatttc ttcatgatga acctcatcaa atggggaacg acaagtgtaa
     3961 tcttcctttt tcttatagaa agagagccta aatatctttt taatattttt ttctttagct
     4021 tcattgattt tattctttca gttgttttta ttttgtctac agaaaagaaa agtcagttat
     4081 tgctgatgaa tggaggttaa caattggagc cgttgatagt gataaaaggt gcgaaaaaaa
     4141 tcattcgaaa caaaacgatt cttcatttgg atcgtttcac tgcggatgct ggggatcttg
     4201 tgactgttgt tggagaaagt ggagcaggta aaagtacgtt attgaatata ttaggattga
     4261 atgatcagtt cagttcagga caattctatt tgttcgatca agagacgaaa caattaactg
     4321 ggagagagaa gcgcttgata aaaagaaata ccatatcttt tctttttcaa gattttgggt
     4381 tgattgaata tgaaacaatc gattttaatt tagatattgg attgaagttt tcgaatttaa
     4441 atgtttcaga taagcgaaca gcaaagtatg aggcattgaa aaaagtaaat ataaacaaga
     4501 agctaagtac gcctatctac acattatctg gtggtgagaa gcaaagaatc gcctgtgcga
     4561 gagtcttatt gaagaatagc aaaatcattt tagccgatga accgactggg tcattggata
     4621 aaagaaataa ggataaagta gttgaaatct tatcgcaaca agcttcccaa ggtacgttag
     4681 ttatcgtagt cacgcatgat ccatatctga ccaaaatagg aaacaagaag atcatgcttt
     4741 aaaaatcaaa aaggaaagga tgtcgtttta tgaaaaaagc agttattatt tgtgcactag
     4801 ggataggttt tgttggagga aacgctatgt catctgaagt ggaagcaaat tcagttcacg
     4861 gtgaaatcta ttacaaatct ttaaagccag gagagattca ttttgatgct aaaggtaata
     4921 ttattaagtc aaatttacca gtagaccggg aaatttctag aacagcaatt aatcatagct
     4981 ctggaagatg ggtttattat agtgatacaa cttgtacgag aaaaacaggt cattctaacc
     5041 actatagtcg tgtctataat cgtcacggtg caaaagcgaa agttggaaac tctattcgat
     5101 cagcaaatgc aacaaaagga gtatgggcct atgcaagtgc gacaggaaat agaactgaca
     5161 cattctcatg ttggtacaat ccaaccggtt ggtattaagt atccaaaagt ttatataaaa
     5221 tagaaaaaac atataatgaa acagc
//
LOCUS       DAZUKC010000134         5118 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000134 DAZUKC010000000
VERSION     DAZUKC010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.140"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(187..1953)
                     /locus_tag="ACG80T_002633"
     CDS             complement(187..1953)
                     /locus_tag="ACG80T_002633"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4842238.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1913..3667)
                     /locus_tag="ACG80T_002634"
     CDS             complement(1913..3667)
                     /locus_tag="ACG80T_002634"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4842239.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLIKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3777..4346)
                     /locus_tag="ACG80T_002635"
     CDS             complement(3777..4346)
                     /locus_tag="ACG80T_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFS4842240.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4364..>5118)
                     /locus_tag="ACG80T_002636"
     CDS             complement(4364..>5118)
                     /locus_tag="ACG80T_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS4842241.1"
                     /translation="TTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITY
                     ENILKDINFDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLV
                     MQEMDYQFFTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLL
                     VSIASLSKTNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNR
                     IIYLKNGGIKKDIKLRVEDSTLIEQIFNDITK"
BASE COUNT         1704 a          957 c          689 g         1768 t
ORIGIN      
        1 ttatcgccaa ttttttaaaa ctttgattat tccttatatt ataagaatag acattaagtg
       61 aactaattca atcatactga ttctttcgtg tattcaaaat tagatgctgt ttaataaaat
      121 tattcttttt gaaaaagaat tataactata tcccaaaaat tgtaggttaa tttaaagcct
      181 atttcatcat cgtgattgag cttctttttc atagacgaat ttctcagata gtttagtttt
      241 ctttatgaga tttttgtata tttctgacct tcgatacaac tcattatgag taccttgatc
      301 aactagtttt ccttgatcca tcactacaat tttatttacc ttttcaatag acttcaagcg
      361 atgcgatata attagtactg tcttatcttg tattaaacga tttaaacttt cctgaatacg
      421 tttttcattt tccgcatcta aactggcagt aatttcatct aaaattaaaa ttggcgcatt
      481 tttcaaaaaa gcccgtgcta tggataggcg ttgtcgctct cctccagaaa gtgaacttcc
      541 gttttctccg accaatgtat gataaccgtc aggtagtttt tcaataaagt cttcgcagtt
      601 agctaaacga gctgcttttt tcacttcctc atctgacgct tgactatttc caatacgtat
      661 attttccaaa atacttgtat taaacaaagt tacctcttga aaaacaatag cgattttgct
      721 aaacaaagaa gctggagata cacgtttaat atcataccca tcaattaaaa tacagccttc
      781 atcataatca tacagtctag acactaattt caagatacta gttttcccgg aaccactagc
      841 acctactaac gcagtcactt ctccttgttt agccgtaaaa gagatatgat ctaaaattgg
      901 ggtattgtta tcatatgaga aagagacatc tcgcaactca atgtcaaaag atttcagagg
      961 actatctgat ccctcttgga tactggtttc cttcatagct ctaattcgct gtattttagg
     1021 agctaaataa aatatttcaa gcactgcttc tttcatagaa tcgaaggaat cctttatttt
     1081 tattgcggct agtaagtaac caacgacgta gagtatagtc acttcacccg ttaacagtaa
     1141 gtgaacacct actagaatga ctactgctaa actaacatat gagaaaatag aggataaggc
     1201 catgactgaa aaagtactta attctacctt taaatgaatc ctttcactct cttccatttt
     1261 tttgaaaagt ctgtcttgaa cttttttaga tagattaaag ctattaatct cttgctgcaa
     1321 ttctatagtt tcttgaaatt cttccgagtt ttctctcaaa gtatcgtaat atttagtatt
     1381 ggctttcgtt tgggatttct ttgataacaa gattaagaca aaactaaata acgttggcaa
     1441 aataacagct aatcccattt tgacattacc aaccaaaagc atcactgaaa taaaagggaa
     1501 aaacagagcc ataccgccgg ctttaggtat tgcatgactc atcgcatgct caataccttc
     1561 aacgtcagac atgatagttt gagataaatc tgataaatta tgttttgaaa aataggataa
     1621 tggtaaattt gacaaatcgt ctgcaatttg cactcttaaa tgcgcacttt ctttataagt
     1681 actattatat aagttttcat actccctatc taacaaccaa tacatgaaaa gcaaagtaac
     1741 aaacgatacg gcaaaataaa gccaacgggg ctttgtattt tctaaaacta attcgtctat
     1801 cagcatcatt ataatgaatg ctggtaccat attaatgcag tatacaaaaa aactagaaat
     1861 agaagcttta gttaaagctt ctgcgccctg atcacttaaa gcaaatttgt ttttataaaa
     1921 acttttcatt gtcaagcctc caatcatttg cattctcata attagtgacc aattgtttat
     1981 acacttgatg tttagccatt aattcattat gatttccacg ttctactatt ttcccttctg
     2041 acaagacaag aatttcatct acagatttaa tgctggataa tcgatgagct atcataataa
     2101 ctgttttttc ttttatcaga tgttgaaaag ccttttgtag ttcatactca ttatctggat
     2161 caatagatgc actggcttca tccataataa caatctttgc atcttttaaa attgctctgg
     2221 caatagctat acgttgtttt tctcctcctg acagataaac tcctttacta ccaattaagg
     2281 tatgctctct ttcgggaaat ttttctaaaa tcgattcgca accggctaat cttagcgcgt
     2341 caaatacttt acttgctggc gcttgaggat ttgctataga aacgttatcg taaatactct
     2401 gattgaatag tttcgtatct tgaaagacaa aagcaattgt gttaataatt gtgtcttttg
     2461 aatagtgatt taaaggctta ccaccaattt taatctgtcc cttatccaca ttgtagaaac
     2521 ctgaaattag tttagctatc gtactctttc cgcttcctga ttttcctact aatgcataaa
     2581 ttcttttttg ttctaaaact aagttgaaat tttctaaaac acactcttct ttgtatgaaa
     2641 agctgacatt ttcaaatgca atgtcacaat ttttaatctg tgtctcgttc ccatatttta
     2701 gttggtcttg cttcatttct aaataaaggg agtctagtgt ttcgactgca tatcttgctt
     2761 caaacaagtg catacttaca tacattattc tcatgaaaga aacaaaaata attccactta
     2821 aaaaaaacaa catcaacaag tcaacagcta atatcttggg agtggtaaga gaagttataa
     2881 aaaaggttat aggaactatc aaaatgcaaa ctatgccaaa aaatagccat tgaaaaagaa
     2941 caaaaggtgt tttcccactc aaagaatact tatatgcaaa cttagcataa tcgttaattg
     3001 ctttattcaa ggctttaaat gatgttactt tagcaccaaa tatctttata acttgaattc
     3061 cgcggatata ttctaccgtt tcagagctta acacatctaa cgattcttga tatttttgaa
     3121 taaaatttgt attctctgtc attttcttta acaagaatac accaattact aacatggcga
     3181 ttagaaccaa tccaatttta aagttaatga aaaagcctaa ggcaactgat aaaacaggtg
     3241 ttactatggc ttgagcacta tctggaatca agtgagcaat tgccatatgt gttttagccg
     3301 cgttgtcatc aattgtcttt cgaatatatc ccgaagaatt taaatcaaaa aaacgaaaac
     3361 ttgcttctgt taaaccgcta attccctttt ttctcaaatt agtctctaag cgaaacccaa
     3421 ggatatgaga aaataagcca gaaaaaatat aaaataaagc tcctaaagtt aaccaagcaa
     3481 cagtttgaac cgcataactc ttagctaaat attcatctcc tacaataatt agagactgaa
     3541 gaaatttgta aatactgtaa tatccataga ctgttaatac gacagaaaaa tcagaaaata
     3601 aagtggctaa ccatccgtag tatttcattt tagggacata actaaataat tttttgtata
     3661 ctttcatttt ttcctccccc gcattcaatt tcttctctta ttcgtttaca actgttaatg
     3721 ctttccgtaa tagcgtaaat tagagatgta caaacagata aaaggtaaac tacgttctat
     3781 tttttgtatt tttttaagac ttttcttcca atcaatacac caatcataga taaacttgct
     3841 acaactaaca ctgaaatgat tccccatata cctaccgcat agtggatcat tgcatccatt
     3901 gttgcagcag attgtccttt atctatccaa tcttgacgaa aacgatctgt aaagaaccat
     3961 ataggaatcc actgtccggc agcactgcct aattgcatta gagcccaaga cgcaacttga
     4021 ggaattgctc gatcatatcc aaatacagat agaataatat cagctaagat cgccatacta
     4081 atcgaactga taaaccaagg taagtacgcc ccgcccatta acgtaaaaaa taacaataaa
     4141 acaattgtgt aaatagtaaa aattcctttt ctagggtagt tatagctaat ataaacaaat
     4201 atgattccag ataataatcc aaaaatcccg ggggatatgc tgtgaccaaa cgccccacca
     4261 atactactaa ccattattga aaaaaagtaa attactaggg aaataactgt agctaaacca
     4321 ataaaaatat aatcttttgt tttcatatat attctcctca attctatttc gttatgtcat
     4381 taaaaatctg ttctatcaaa gttgaatctt ctactctaag ttttatatct ttttttattc
     4441 caccattttt taaataaata attctattgc atgttttata aagaaattcc ggatcatgag
     4501 tagctataac tactgcaccc ttttctttta agtctttaat aaggtcagca atcctttcca
     4561 tattaatata atccaaccca cttgtaggtt catcaaatag aaataaatta gtttttgaca
     4621 atgaagctat agaaaccaat aatctttgtt tttctccacc ggataaatca aacggtaatt
     4681 gatctttcat tttataaagt tccatttttt ttaaaatttt ttcttgctga tctttactaa
     4741 ctatttcatt ccccagggct aactcgctcc tcactgattc tgtgaaaaat tggtaatcca
     4801 tttcttgcat tactaaaaac ggcgatgaca aatttttttt attttcgatt atctttcctt
     4861 tggtagggcg aataatcttc atgagtattc ttagtaaggt tgttttcccc acaccgtttt
     4921 tccctaccaa accaataaca tctccaactt tgacatcaaa atttatgtct tttagaatat
     4981 tttcataagt aatttcttca acttttaata catctgcact taccttttcg atatagggaa
     5041 cttcaatcga actattaaaa atgtcaaatt tccttaatgc gttattgttt tcagttttta
     5101 acaactgaat agttgtag
//
LOCUS       DAZUKC010000135         5011 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000135 DAZUKC010000000
VERSION     DAZUKC010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.141"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..647
                     /locus_tag="ACG80T_002637"
     CDS             <1..647
                     /locus_tag="ACG80T_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4842242.1"
                     /translation="GAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIVLMNFINPILGLI
                     VVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLILSSVGYMGLGVF
                     NTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVGGWTLSIIGYDSL
                     ANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENCELLKQKHK"
     gene            657..1451
                     /locus_tag="ACG80T_002638"
     CDS             657..1451
                     /locus_tag="ACG80T_002638"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4842243.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            1464..3230
                     /locus_tag="ACG80T_002639"
     CDS             1464..3230
                     /locus_tag="ACG80T_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFS4842244.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            3253..>5011
                     /gene="uidA"
                     /locus_tag="ACG80T_002640"
     CDS             3253..>5011
                     /gene="uidA"
                     /locus_tag="ACG80T_002640"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFS4842245.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAY"
BASE COUNT         1799 a          599 c         1008 g         1605 t
ORIGIN      
        1 ttggtgcggc tattttctta ctccttgctc aattattaat tatgagtatg aataattatg
       61 tatttcctga ttactataat gatgcacagg gaatagtttt aatgaatttt atcaatccta
      121 ttttgggttt aatagttgtt tcgcctttaa ctttgttttt aagtaaaaaa tatggaaaaa
      181 aagaactggc atcagtagga atgttctttt cagcaatagt atatttgatc ttatttttga
      241 taaaagctag taatatgtat gtattcttaa ttcttagttc ggttggctat atgggtttgg
      301 gtgtgtttaa tactgttatt tgggcaaata ttactgatgt tattgatgat caagaagtaa
      361 agtctgatca aagagaagat gggaccgtat atgcggtata ttcttttgct agaaaaatag
      421 gtcaagctct agctggaggc gtcggaggtt ggactctttc tattataggc tatgactctt
      481 tagcaaatgt acaaacggga gatgttttag aaaagttata taacgcttca acattaattc
      541 catcaatttg ttttctgatt gtaggattaa ttttattttt cctttatcca ttatctaaag
      601 ataaagtctt agaaaattgc gagcttttaa aacaaaaaca taaataggag agaaatatga
      661 ataattggat attttgtatt gattcagatg gctgtgttat ggatacgatg acttataaac
      721 acaatctctt tttcggacca attgctgcag agatttttga tgttcatcat agagaaaaat
      781 tcctacagga ttggaataat attaatttat attcagagac tcgtggaata aacagatttt
      841 taggtttagt taaaacatta gaaaaaaatg atgtaacatc gattaatcga ttaaagcact
      901 gggtatctct aacaaaagaa ttgtctaatc catctttgaa gagggaaata gaatattctc
      961 agtcagagga tttattaaaa gctttaaaat ggtcggaaga agtcaatcaa cagattagtc
     1021 aagcaaaagg attagataaa ccctttttaa gtgctagaga tactataaaa gctctgaaaa
     1081 aatatgggaa gataattatt gtaagctcag ctaataagga agctgttcaa gaagaatggg
     1141 aaagacatga attattatca ttagttgatg aactgtgttg tcaagataaa ggaaaaaaag
     1201 aagatattat tcgaagtgta attgaaaatg gttgtgattt ggataagata ttaatgattg
     1261 gtgattctcc tggtgatcta gaagctgcaa ataaaaataa agttttcttt tatcctattt
     1321 tggtcaataa agaaaaagaa tcatgggaaa acttaagaac tgatgtttta tttaaatgtt
     1381 tatcgggaga ttatgtagat attgaacaaa aatatataga tactttttgg aaaaatctta
     1441 ctaataagta gaggagaaag aaaatgacta taaaagtaga tttacgaaaa aaaccatata
     1501 atttagatga agatgctatt tcttggattg aagagacgat tgattctatg actttggaag
     1561 aaaaaatagg tcaattgttt gttaatatgg gatctagtcg tgataaaaat tacttgagta
     1621 atatggtcaa tcagtaccat attggagctg tacgatataa tccaggaact gctgatgagg
     1681 tatatgatca aaactatatc ttacaaacag aaagtaaaat tcctctatta attgctgcta
     1741 atacggaagc aggtggtaac ggagcttgta cagatggtac tgaagttggt gtagaagtaa
     1801 aaattggtgc aacaaatgat gcgaaatatg cttatgaatt agggagagtt tctgggattg
     1861 aagcatcggc tattggatgt aactggagtt ttgctcctat tgtagatatt aatagaaatt
     1921 ggagaaatcc tatcatttct agtcgatctt ttggacaaaa ccctgataaa gttttagaac
     1981 ttagtttagc ttatatgaaa ggtattcagg agagtggaat tttgcctgca gctaaacatt
     2041 tccctggtga cggtattgac gaacgagatc agcatctatc ttttagtatt aatagtttag
     2101 gtacggaaga atgggataat tcttttggaa aagtatactc aggcttaata gaagctggat
     2161 taccttcaat tatggcaggt catatccata tgccagctta ttcaaaacat tttaatcctg
     2221 atctgaaaga tgaagattta cttcctgcaa ctttatcaaa agaattaatc actgacttac
     2281 ttagaggaaa actcggattt aatggattag tagttactga tgctagtcat atgttaggaa
     2341 tgactggggc aatgaaacga tcagaattat tgcccacttc tattgcagct ggatgtgatc
     2401 tatttctatt ttttaatgat ccagatgaag actttaatta tatgatggaa ggctatcgta
     2461 aaggaattat cactgatgat cggttattag atgccttaaa aagaatttta gggttaaaag
     2521 ctaaattggg attacacaaa aaagcaaaag aacaaatttt attacctaag caagaagctc
     2581 tggaaaaaat tggtttaatt gaaaataaat caatttttaa agaggtggct gaccaagcaa
     2641 ttacattagt taaagataaa caagatattt ggcctattgt accagaaaaa tataaaagga
     2701 ttttactagt agatgtacat ggggtaaaag gtgggtttgg tgaagtagtt gctggaaata
     2761 gacctcaagc cattgattta ttgaaacaga ttttgtcagc aaaaggattt gaagttgaaa
     2821 aatacatttc acctgaagaa gaattgaaag gattatcgca agaagaaaaa attaaaaata
     2881 tagctggaat atatgctgct aagagaccaa ttaaaaattt aactaaaaaa tatgacttga
     2941 tcattaatat agctaatgtt aatccaggca cagttcaacg aattgtctgg ccagcaagta
     3001 aaggaactcc ggatataccg ttttacattc atgaaattcc aacaattttt gtatctgtac
     3061 agtgtccgtt tcatttggct gatgtacctc aagtaaagac ttatattaat acttatgatg
     3121 gaaaacaaga aactttagaa atattggtgg acaaactaat gggtctttct gaatttaagg
     3181 gagtaagtcc aatagatgct tattgtggtt taaaggatac atttatttaa tatttagata
     3241 ggagtggttt atatgttgta tcccattatg acagagacga ggcaagtaat tgacttaagc
     3301 ggtctctgga gatttaaatt agatacaaat gatgacttac aggaagaact tgctttagaa
     3361 ccaataaatg aaaaagagag ttatccgatt tcagttccct cttcttataa tgatttattt
     3421 gaaacagaga acattagaaa tcatgttgga tgggtgtggt atgaaaaaga agtgactctt
     3481 cctaaaagat tattagaaga aagattagtt cttcgttttg gatctgcaac acatgaagca
     3541 aaagtttttt tgaatggaaa gtttattact caacacaaag gtgggtttac accatttgag
     3601 tctgaaatta atcggtttgt aaaagacggg aataatcgaa ttacagttgc tgtaaacaat
     3661 attattgacg atagtacttt acctgtgggg attatgaagg aaacagtaca ggaaaatggg
     3721 gatataaaaa aggaaaactt agttaatttt gatttcttca attatgcagg tattcataga
     3781 cctgttaagt tatatacaac tccgaaagac tatataagtg atattactat taatacaaga
     3841 ataactgatt tggatgcaag tgtagaattc aatatcgata cagaatggac cggaaaaatc
     3901 ttaatagcga tatatgacga agaggctcaa cttgtagctg aagctgaagg aaagaaagga
     3961 atgattcagc ttaagaatcc aattttttgg gaacctctag acgcttattt atatagtatg
     4021 aaaattagtt taattgatga taataaagtg atcgatacct atacagaaga atttggtatt
     4081 cgcagtgttc aggttaccaa tggcaaattt ttgattaata ataaaccttt ttattttaaa
     4141 ggatttggta agcatgagga ttcgtatgtt aatggacgtg gtttgaatga ggctgtaaat
     4201 attaaagatt ttaatttaat gaaatggata ggtgctaatt cttttagaac atcacattat
     4261 ccttactcag aagaaattat gcgattagca gatcgagaag gtattgtagt tattgatgaa
     4321 gtacctgcag tagggttaca tttaaatttc atggccactt tattagatga taatgtggaa
     4381 aaacataata cgtggaaaga gatacgaaca gaaaattctc ataaaacggt gatcaaggaa
     4441 ttgatagaac gcgacaagaa ccacccaagt gttgtaatgt ggtctattgc aaatgaacca
     4501 gattcggatt ccgaaggtgc taaagaatat tttgaacctt tagttgaatt aactaaagaa
     4561 cttgatccgc agaaaagacc tgtaacaatc gttacttatc taaaatcaac gccagatgtt
     4621 tgtaaggtag gagatattgt cgatgtttta tgtttaaatc gttattatgg ttggtatgtt
     4681 tcgggagggg atctggcaca tgcaaaatat ctgttgaaaa aagagctaga tggatggatg
     4741 gaaagatgtc ctgatacgcc aataatgttt acagaatatg gagcagatac agtagctgga
     4801 tttcatgata cagtaccaac aatgtttaca gaagagtatc aagtggctta ttacgaagca
     4861 aatcacgaag taatagatac atgtgaaaat tttgttggtg aacaagtgtg gaattttgca
     4921 gattttgcaa caagtcaagg gattttaaga gtgcaaggaa ataagaaagg aatctttact
     4981 agagatagaa aacctaaaat gattgcttac t
//
LOCUS       DAZUKC010000136         4812 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000136 DAZUKC010000000
VERSION     DAZUKC010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.142"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(92..1177)
                     /gene="xerS"
                     /locus_tag="ACG80T_002641"
     CDS             complement(92..1177)
                     /gene="xerS"
                     /locus_tag="ACG80T_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFS4842246.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            1526..1721
                     /locus_tag="ACG80T_002642"
                     /pseudo
     CDS             1526..1721
                     /locus_tag="ACG80T_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
     gene            complement(3253..3771)
                     /locus_tag="ACG80T_002643"
     CDS             complement(3253..3771)
                     /locus_tag="ACG80T_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFS4842247.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            4138..4386
                     /locus_tag="ACG80T_002644"
     CDS             4138..4386
                     /locus_tag="ACG80T_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842248.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         1580 a          787 c          732 g         1713 t
ORIGIN      
        1 atataaaaat gataatgaag cttgccttta aagttgggct tttatctaat tttagagaca
       61 ggcttcatta ttatactatt ttttactaca tttacaaagc gttcaaagca tcaattgtag
      121 ctgcattgtc tatatgagta tataaagttg ttgtatttgt acttgtatgt cccaattgtt
      181 gggcagttaa taccaatgaa tttgtctgtt gatagagtct agtagccaaa gtatgacgta
      241 atttatgtgg actgacttgg actctgaatc cttttgaata ttttgctacc atccgttcga
      301 ttgcaatacc ggatatacgc ttggcttctc cttgataaat tgctagaaat aaagcttttt
      361 cgttttctgt tactttgtat cgttctttcc gaatagctag ataattagct aaatattcca
      421 ttgcaaatgc tgctatattt actgcgtctc tcttatttcc ttttctagtt actaccacat
      481 tattcttttc taaagatagg tcctctaaat taatatttac aacctcggag agtcttaatc
      541 cagatcctaa aatcaatgca ttgatcgcca ggtctctttc tacatctcgt tgataataga
      601 tcaaagctcg tttgctcaaa gtttttttat attcattggc aatataatct agataatttt
      661 gtgtatcttc gtctagaaat aattttttct cgattgcgtt tgcacgggca gctaacgtag
      721 ttttatctgt gatagttgct acttgatcca taggattatt ttctaagtat ggttttccta
      781 aaggattatt ggaacttttc gaaagatatt taaatagaac ctttaatgct gtgatatttc
      841 tattgatagt agaagtagat agacctaagt tattattatc tttgagctta gtttttgttg
      901 tctctttttt tacattttcc tttgtgcgac gtttcaaatg agatttgtac aagtttagat
      961 cataggcagt taactgtctt atcgaggtga ttgaagtatc gggaacgata gactcactca
     1021 gaagccattc ataaaagagc ttataatttt ttagatattc taaagtagtt cgaggtgatt
     1081 gatctttatc ctcacgatct tgaacaaaat cttttatata atcgggcata tgaagtatga
     1141 ttgtatctat tttttgatat atttctgtat ttttcatgtt attcatcctt tcgataagta
     1201 ggggagagga gtatctctat aattggacta taccaattat agagtgtttt gtcaattggt
     1261 atactctgaa atttattaat ttatttcaaa aaagcagata aataggtata gatttaataa
     1321 tttaaaataa atggtataca tgaataggaa aggaaacaga aggaggcaat cttaattttt
     1381 taaattgtct aaattatata gaaagaacaa tctttctata taattagaca taagagggac
     1441 tacggttgat ttgatattaa caagcgtata tgaatgtata tacaaataaa aaaggggagg
     1501 gagaagttaa agaaaattca gagaagtggg gcactcagta gtattaacaa tttaaaagga
     1561 atttcttaaa gaaaatgggt tgcatcctgg agatacaatt tttttagata aagaaaaaat
     1621 tcaagcaaca attcaaaaaa atcttaactg ctgagattga tgtagcaata tctcaatcat
     1681 caacaaagta cgatcctgcc tttcaaaagc ttattgataa atgtttattc attataaaaa
     1741 taaaagaaaa atttatgtca gcttttttca tcaaagtggt aatgataatt cgtctattaa
     1801 ttgtattttt taggaaatat ggtataattg ttttaacgaa aaggtaattc atgatacacc
     1861 aaaaaccact agctggcggg ctagtggttt tcttttacgt tctattcaga ttaacgggta
     1921 ggcttactgt cttttttggc aataattttg caatcaagcc atttacagat gtaatgcgcg
     1981 gatacttctg ctaagattgc aaaaaacata tcgaaaaggt aattcataat acaacctcct
     2041 ccccttgcca taataggccg gggtagcaga cagcataaat attataccat aactgaaaat
     2101 ttcattttag tacatttcaa tatattgatg aaccagcaca ataaaacatg ccatgactaa
     2161 cagaaaaaaa cttattaaac cagatataac ttgtaaacat ccaactatat agaaaacgta
     2221 aattttctat atagttggat taagataacg actatttatt ttatcatatt tttactatac
     2281 cctatacttt tacttggtat atttatgaat aatcgcaaat ttacccctat actactagtt
     2341 tgtaagttag attaataaaa atactaaagt tatttgttct tttattaaat acacctacaa
     2401 cttataaatt gatggtattt agataacacg aaagaataaa aataacagct gatagtaaag
     2461 tttgttgacg attgctactc aatcgaaagt gagttagtgc gtatgaactt gcttctgaaa
     2521 acttatgaaa tgaaagacct tttacatctt gcagaaatcg gtactgggat taaactaagc
     2581 aataaaaact aatgttcccc tcattctttg aacttctact atttgattta tttattctga
     2641 taaatgacga tttcaccttc aggatcttca aatcccgtat agaatttatt taagatctct
     2701 tcagtatcag taataggata ggcgataggc cacataccct ttgatggtta gaataaactg
     2761 ctcaaaatct gtatcgtatg ttggctttag catattaaca aaggctatct ccaaattata
     2821 aaccataagc gttgaatcgt tcttaacaat ttcaattctt cctaacccaa agtcagaaga
     2881 tataattacc ggacttgcct tacccttcat gtgggttgtt gagattccat atttaaaggg
     2941 aattgtcggt actatcggcg gtctgaaaaa catcccaaaa ataaaaaaat ctttcccctg
     3001 ctagtctcct aagtcccttt gtagcggata tggcaccata atctcatttt atcgaagtga
     3061 gtcatggttt atctgttggc caaccaaaat tgatacaatc taacgatgca ccctctacgg
     3121 cattctaggg gagtgcactt gcattcaccc aataaaaacc ggcagttagc aaagaactta
     3181 tgtagtcgta tgttcttact gttaacatac aagaggtctc ctttccacaa ttgcttagca
     3241 gtaaacggga atttacaaag ctaatctctt ccattttctt atctttgttc taaaagtccc
     3301 aaatggagca actgtattta catgaataaa tttatatact tcccatacag ccgttttggt
     3361 tgcctcgtct gcccattttc ttttgtgtgc agtgaataga tcttcatcac tcattgaatc
     3421 aatcatcatg ttaatatcat caatattacg attgagaatt tcctctaatt ctttcaatgg
     3481 tagatgtgca taggtatttg taaaccactt atataactct cctaactgat tccatttaaa
     3541 ttgatcagaa ggcgttttaa cagataatcc atttctttca tcattttccc attttagaat
     3601 taaattcgtc caaccaactt gatatgctaa attttcagca ggtgttctat ccacttcctc
     3661 aactctttta tccttcaatt cttcaggaat agtttcaaat tctaaaatat atttagtata
     3721 agtcctattt atttcttttt ttaattcttc tttattttta tatgttctca ttatattacc
     3781 tccataaatt cctaattgtc tcctttaatt catctataat ttcatccacg atattttaat
     3841 ccgttctaca atgcatatct gatatgcatt gactgtctaa ctcatatgtg aatgatttat
     3901 aaaatatccc aacgtctcat ctaaagaaat gttgatcgtc agcaaggagc taaaaaatac
     3961 gaatatattt cttttgtagt aaaatcggac tggtttgcat atcattttct tcgtaaatat
     4021 caaaatcatg ttaactacta tcaattacta ataaaaacta ccactttaag cgttaagaac
     4081 tgttaagttt agttaagtga ggatagttca caaaatccat tgttaaactt cacgaaagtg
     4141 gtaaaactca aaatgcttta tataggatta cagcatcgct ctttcttcga ttgcgcgttg
     4201 ggtaaaaaat attatgaagt aaaaattgac gatgacacgt tccttacggc tcatcagatg
     4261 aaacagctac aaaaacgaaa tgatcaactc gaagaaaatt taattctaaa aaagcgattg
     4321 ccatattcac gcctcgctcg acgaactatt aaaggtcaag atttacctga agatcatcct
     4381 ttttaggaaa ttgaagcttt ttatgttgca caatgcattt ataatactac aatgatgttt
     4441 tcaccagata ttcttctagg gtgcggtgta atgaaacaga tacacttgca aaagaaaatt
     4501 caccgcgaat ttactaaaat tatgaataat tatgttaatc ttccgaaatt tttttccaaa
     4561 attaagggat caagctggtg ttatagattg tttaaggttg gcaaaaaaat aacttctttt
     4621 taaacaatta tagaaaaccg atactctgtt aagttggttg acacggtgtc gatttttatg
     4681 actttcttat tctgaaactt cactaacgga cagtacctaa aaagtctata gtatggacgt
     4741 ttacgagtac tattttatca aaaatgctgc tcatttcaac ttatatgttt tagtgaacta
     4801 cccccactta gc
//
LOCUS       DAZUKC010000137         4796 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000137 DAZUKC010000000
VERSION     DAZUKC010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.143"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..527
                     /locus_tag="ACG80T_002645"
     CDS             <1..527
                     /locus_tag="ACG80T_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS4842249.1"
                     /translation="KQYRTKGIAYFSKEDFHELLDIPKSYRESVINKKIIKPIRQELT
                     AIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQI
                     YSEKDPTSLQEKIKEQKRKLELLQQLEELERKQNNLNKENKEINEQIPDNNSDIPPEL
                     KKGLLDNLQNLFKE"
     gene            537..1034
                     /locus_tag="ACG80T_002646"
     CDS             537..1034
                     /locus_tag="ACG80T_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFS4842250.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            1410..1691
                     /locus_tag="ACG80T_002647"
     CDS             1410..1691
                     /locus_tag="ACG80T_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842251.1"
                     /translation="MDILVRKIDSKIISEIDRRCQELTTKTSKKWSRNTYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNEYVRTNNQLIETLIGGL"
     gene            1693..2109
                     /locus_tag="ACG80T_002648"
     CDS             1693..2109
                     /locus_tag="ACG80T_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842252.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISILSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNHQKETNQTFTAGNFDPTNFLKR"
     gene            2767..3192
                     /gene="bacA"
                     /locus_tag="ACG80T_002649"
     CDS             2767..3192
                     /gene="bacA"
                     /locus_tag="ACG80T_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="HFS4842253.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            3222..3386
                     /locus_tag="ACG80T_002650"
     CDS             3222..3386
                     /locus_tag="ACG80T_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842254.1"
                     /translation="MKLTKIAVILAVIAGIILYFVLPANQFFLYFGIPAILIVNGVRI
                     FEQRKKCSST"
     gene            4346..>4796
                     /locus_tag="ACG80T_002651"
     CDS             4346..>4796
                     /locus_tag="ACG80T_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS4842255.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRL"
BASE COUNT         1745 a          816 c          804 g         1431 t
ORIGIN      
        1 taaaacaata cagaaccaaa ggaattgcct atttttcaaa agaagacttt catgaattac
       61 ttgatatccc taaaagttat agagaaagtg ttataaataa aaaaattata aagcctattc
      121 gccaagaact aaccgcaatc ttcaaaggtc tgacaatcaa aaagaaatac ggaaaaggaa
      181 gaggaaaacc tgtcattggc tatcaattca cgtttaaacc tgaaataaaa aaagcagacg
      241 actttaataa acacgcacca acaaaagaag aacaaaataa agctttagaa ctagttaatc
      301 aacaaattta ctctgaaaaa gaccccactt cattacaaga aaaaatcaaa gaacagaaac
      361 gaaaattaga acttttacaa caattagaag agctagaaag aaaacaaaat aatctgaata
      421 aagaaaataa ggagattaac gaacaaatac cagataacaa ttccgatatt cccccagaat
      481 taaaaaaagg cttactagat aatcttcaaa acttatttaa agaataaggg gtttttatga
      541 cagataaaac tatcaaagaa ttagcagata ttctaggtgt ttcaaaagat aaagtgaagt
      601 atcaactgag gaaattacct agtaatttta cctacaaaaa aggaaatatt atctacctga
      661 aaaaagaagc agtaacaaca attacaaagg ttttatcagg taatgagccg ggtaacttac
      721 ctagtgaatt acctgattca ttacctaccg attacctact caaggagatt gaaactaaga
      781 ataatcatat tgaagaaaaa gataggcaaa ttgaaaaact tcaactagct ttagaaacac
      841 aacaaaaatt actcgaccag caacaacaac tcaccctgca agccaaccaa caaatcgaga
      901 aactgcagga acaactacag ttgacttata cggaagaatc ttcggaaaat aaatcaacga
      961 ctctttctga agaaacagaa tcgatagaaa aacaatcaaa gacggagaaa aaatggtggc
     1021 aattctggca ataaaaccag caatttaaat taataaaaat tgctggtttt attcttcttt
     1081 ttgcttttgc gagcagagca acatgggccc atgttgcgat attttggtgg cgccccaatc
     1141 cccgtttttt cgttgaatct tactcggact gcactcgttt tacagctgat ttttctgaat
     1201 tttgaaagag cataaggaaa cgtggtatca ctttcttacg aaaggtgcta gataagctgt
     1261 gcctatcacg tttctcagaa aaaaatttgc tatcactttt ttgccattat ggatagtgta
     1321 tgtatagtat tttggtaaaa aacagctata caaatactat atttgtatga tatacataga
     1381 ttatacgcac atgcgaaagg aaaattgcta tggatatttt agttcggaag attgattcaa
     1441 aaattatttc agaaatagat agacgttgcc aagaacttac aaccaaaacg agcaaaaaat
     1501 ggagccgtaa tacgtaccta aaagttctaa tcgaaaatga ttttgaccag gcacttaggc
     1561 attacaaaaa agatcaattc gatttattac tcgaaaaatt tatcgatata caagcaaaaa
     1621 atacggaaac actgaatgaa tatgttcgta caaacaacca actcattgaa acacttatcg
     1681 gaggactata acatgagaaa aacttctgtt caaaaacacg ttacccttta tcccagtcac
     1741 gtggacgttt tagattccat tattgaaaga aatccagata tcaacaatta ctcggaagcc
     1801 attcgttttt tatgtatgag ttatgaacct gataccaaca caaccactac tagcaaccaa
     1861 gccaaactaa atgctatggg aaaagaaatt tctatcctgt ccgaaatcat tgcagaattt
     1921 gctgatttat atctaaaaga tactggtgtt cttattggta ccaattctaa ggtgtatcaa
     1981 gaagtgaaag aaaaaattga aaatcaactc aatccttccc gacgaaatca acccaaaaat
     2041 catcaaaaag aaaccaatca aacattcact gctggaaatt ttgacccaac caactttcta
     2101 aaaagataaa aacagaacct gaaaaataaa aaaattcctc gatttctttt tagggggggc
     2161 ggtcttccag caagatttgc aagttgggac caacttgcgg aaaaaaagaa aaattcgctt
     2221 gcgcgaatcc ttctttttgc attttggggc gctgccccaa tccccaagta tcggttattt
     2281 aatagcgtgg caactcaaag aacagaacat actctgaaac aaaaaaagac tgctctaacg
     2341 agcagccagt taaaaaataa tgagtgttta ttcttgaaaa agcaacgcgc ttatgaagcg
     2401 ctaccaaaaa attatcatat tttattaaaa ataacaaccc agtaaaaaat gactggtaaa
     2461 aattcacaaa gaaacggaaa gaagcaaccg cagaaaatca agcttaaaaa gagcttttga
     2521 cctagaccaa tgaaaaatca caaagtccgc ccctgtacct gttggtagct gggctacttg
     2581 gcatactgta cattatgtca acaagaattt aatataacat atttttctag tttgttataa
     2641 caatgccact ctaacaaaaa tgtaagcatt atccttatta aaagctcctt gacaagacta
     2701 taccttttat tttatgattt ttacatagaa aacgttttct ctattttttg tttagaggtg
     2761 atttttatga tttgcaagaa caagaaaaaa attattgcta ttattgctag tttctctttg
     2821 atttttagta ctgcatgtac tgtcttatta acaaataatg ctaatgttga agctgcaagc
     2881 tcttcttaca aacataatca tgctggttat agatgttaca aaatgagtta ttatgtaagt
     2941 ccttctaaat gcaaacaatt ggctcgccaa actaataaac ttaataccgt ctcagctatt
     3001 gcaaacttta ttggactatc tggattaaca ccggggatta tatcattagt tttcggatca
     3061 gcagttagtg ctaataatgt atttgttaca gccgctagaa gaggaaaagg cgttcaatta
     3121 aattatattg cccattttag taataccact acctataatt atagttctaa ccaaaattat
     3181 gttataaaat aattaaaact ttaaactagg aggaatgctt tatgaaatta acaaaaattg
     3241 ctgtaatttt agctgttatt gctggcatta ttttatattt tgtattgcct gctaatcaat
     3301 tcttccttta ctttggaata cctgcgattt taatagtaaa tggcgtaaga atctttgaac
     3361 aacgaaaaaa atgtagctct acctaatcgt tacaaaacaa caatttcacc ttcggtgaaa
     3421 taagcgaaac tttacaaaaa gtggctagca aatgctagcc ttttttgtat tgtagagcgt
     3481 gaattttgag ggggattcaa gggggagctt gcgccccttt gacaagcggt tatttgcacc
     3541 aaatacctcc gtatttggct agcaatcctt ctaaaaaata cacctatcga tcagagtcaa
     3601 aacaaatcaa ttttcgagtg agcgaatccg aatttgcaaa gctgaagctt tgcaaatgag
     3661 tgtgccggct tccgtaaaaa tacatatggc tataatttgc gttttaaggc gttttaaacg
     3721 ttctggtata taaaattata ccaaaacaac tttaaaacgc cttagatcta attttagagg
     3781 tcttatagaa cattttaggg ttgttagggc ttgccctgac cgcctgtaag gcgctttgag
     3841 aacaagataa gggttttata ctaagactgt cttaaaacgg cttaaacaaa cgctcagaac
     3901 ttttcaaagg agttctttat gaactgtttt taagcaaagg agcctttagg cgactgcgca
     3961 gtataatgtg agagcgtagc gatcacacat ttttagtgac cgtagggagc gagtttccca
     4021 gtttagaaaa attcgcgaac ttgtttgagg aaaaaagcag gagtttttta ggcgggaaat
     4081 tttgcctcct cttttataat ctctttttaa acctcttttt aaacctcttt accgtcggct
     4141 gtatgcttac tctcccaagg tgtgcagaat cttttggggg tctttttgtc tgtttttagg
     4201 ggtctttttg tctgttttta ggggtctttt tgtctgtttt tgggggtctt tttgtctgtt
     4261 tttaggggtc tttaattagg ggcttgaccc ctattagaaa gctctttata attttgttaa
     4321 gtagttaatc ttgaaggagg attttatggc aaatgaaatt gtgaaatacc accatgaatt
     4381 aaatacgatt cctttacgca aatttacgtc agtagaaatg aatttattct tctctatagt
     4441 atctagaatg cgtgacgtag gcgataaaaa agtacaattt acattcgaac aattaaaaga
     4501 tttaagcaat tataaagcaa ctgcaaatgt tcgattcata gatgatttag aaacaacgta
     4561 tgacaaatta atggatttac gttttggtag aagaagtgct gacggtttgc aacgtgaacg
     4621 atttgtttta tttaatcaat ttaaaatcga tggaaaagct gatattcctt ttgctgaaat
     4681 acaagtccat gagaaagcct tacccttact taataatctt gaagaatggg ttcgatattc
     4741 tctccaacaa ttcaatgaat tagaaagcag ttattccaaa accatgttta gattat
//
LOCUS       DAZUKC010000138         4730 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000138 DAZUKC010000000
VERSION     DAZUKC010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.144"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            95..1054
                     /locus_tag="ACG80T_002652"
     CDS             95..1054
                     /locus_tag="ACG80T_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS1252 family transposase"
                     /protein_id="HFS4842256.1"
                     /translation="MTYTHLTTDELVMIESYYHQNIKIVKIAEYLKRSRTPIYNVINF
                     LKEGHTALEYYQQYKENKKRCGRRNIVLPKKQQSYIKEKIAQGWTPDVIIGRKEKPIS
                     CSVRTLYRQFKEKVFDESTLPMKGKRKPNGHQERRGKQAFKRNIVERETDYPTFKEEF
                     GHIEGDTIVGIHHKSAVITLVERLSKIIITLKPEGRKAIDIENTLNHWFGSIPRHLFK
                     SITFDCGKEFSNWKALSNQHDIAIYFADPGTPSQRALNENSNGLLRKDGLPKNMDFNQ
                     VDQAFISSVANKRNNIPRKSLDYQTPLEVFMSYMNEDILSSLI"
     gene            complement(1487..2059)
                     /locus_tag="ACG80T_002653"
     CDS             complement(1487..2059)
                     /locus_tag="ACG80T_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4842257.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2075..2680)
                     /gene="fic"
                     /locus_tag="ACG80T_002654"
     CDS             complement(2075..2680)
                     /gene="fic"
                     /locus_tag="ACG80T_002654"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFS4842258.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            complement(2860..3597)
                     /locus_tag="ACG80T_002655"
                     /pseudo
     CDS             complement(2860..3597)
                     /locus_tag="ACG80T_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336528.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(4040..>4730)
                     /locus_tag="ACG80T_002656"
     CDS             complement(4040..>4730)
                     /locus_tag="ACG80T_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TnsA endonuclease N-terminal domain-containing
                     protein"
                     /protein_id="HFS4842259.1"
                     /translation="YQYSNLTLIAEIARYLNFSPLEVQRILTESQDGLEQILFWSNTY
                     KQVVYEILIPKIFEYKFKITKSQITEDNEVVLLKFPQGKNYYKFSGKPELVIMDSDKQ
                     LENYKTKSFHADTYVFDSKPERELFLQYLENDTVEEIFFTGMFTSKQGDFYIQYIDPE
                     ANSVRSYFPDFVVKKRDGSREIIEVKGDNKKDDPVVLAKKAATEEQAGQSGYSYRMIL
                     GSEIMNNNYEI"
BASE COUNT         1436 a          916 c          804 g         1574 t
ORIGIN      
        1 gccgattgta aaattaagct agacaaataa ataagccatt cgtgctacac tcaaaatgta
       61 attccgacta aagaaaacat aggagtgatc acgaatgacc tacacccatc ttaccacgga
      121 tgaactggtg atgatagagt cttactacca ccaaaacatc aaaattgtga aaattgctga
      181 gtacctgaaa cgatctcgaa caccgattta taacgtcatt aatttcttga aagaaggcca
      241 tacggccctc gaatactacc aacagtataa agaaaataag aaacggtgtg gcagacgaaa
      301 catcgtcttg ccaaagaaac aacagtccta cattaaagaa aaaattgccc aaggctggac
      361 gccagacgtt atcattgggc gtaaagagaa accaatcagc tgttccgtaa gaacccttta
      421 tcgccagttc aaggaaaagg tgttcgatga atcgactttg cccatgaagg gtaagcggaa
      481 accaaatgga catcaagaac gccgaggaaa acaggctttt aaacggaata tcgttgaaag
      541 agaaactgat tacccgacat ttaaagaaga gttcggccat atcgaaggcg ataccattgt
      601 aggcattcat cataagagtg ctgtcataac tttggtagaa cgtctatcga aaatcatcat
      661 caccttgaag cccgaggggc gcaaggcgat cgatattgag aatactctga accactggtt
      721 tggatcgatt cccagacatt tatttaaatc cattaccttt gattgtggga aagaattctc
      781 taattggaag gctttaagta accaacatga catcgctatt tatttcgctg atccagggac
      841 tccttcgcaa cgagcactga atgaaaattc caatggcttg ctgcgaaaag atggtctgcc
      901 aaaaaacatg gattttaatc aagtcgacca agcttttatc tcttcagtgg cgaataagag
      961 aaacaatatt ccacgaaagt ccttggatta tcaaacaccc ctggaagtat ttatgagtta
     1021 catgaatgaa gatattttgt ctagcttaat ttgacaaatc aaattattta aaactttgca
     1081 acagaacctt agcagcagtg ctgattttta tgatagttat ttggttctta aatgaaagat
     1141 ctaaagacaa tcaacaccgt agggatatgg agataaaatc aatagagtca atagattatt
     1201 tatcgataat aacactaaaa aagccacgtt cctaaacgtg acttacgctg aacgatatgt
     1261 gttgtttggt agacgaacaa atcgttcatt ataaatatat gatggtatcc taatatagta
     1321 agcgtggcta gcataccttt aattacttta ccttttacag ttaccataca cattttttga
     1381 gcagaaatca agtaaataaa aaaatattat atttcacaac atgctaatag actcatatct
     1441 agaacgcatt aagtatttac ctgtctggtt cgctgagact tcttttttaa ctttcttcaa
     1501 ttttcttctt tattctaaaa agcgtactgc gactgaaacc ggttatagct tcaacttcct
     1561 tatacgattt acctgaagta agcagttcat atgcgtgcct cttcttcggt gtgattgttg
     1621 ctttcggacg tccttctctg aaattaggat tattctttct tgcaaacatt tttccttctt
     1681 gggtacgttc cacaatcata tcacgttcca tttctgctac actcaacaac gtccgtgtca
     1741 ccattcgccc catggacgta ttttcaatcg ttcctagatt caaaaccttg atcgttacgt
     1801 tatcatcgag taaaggttca atgatctcta atgcttctct cgtgttacga gcaaatcgat
     1861 caagcttcgt aacaactatt tcatctccag gcttcagacc agccaacagc tgattgaatt
     1921 ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa aatattcttc ttctctacgc
     1981 cgttctcaag caactgttga atttgttctt ttgtactttg agaagcggtt gagacacgtg
     2041 catatccata tttcgtcatt tggtccacct acttttacaa atcatctatc gtatactcag
     2101 aatacccttc atagaaatag ctgacatcga ttcctttcat atataactcg cgatcgtcaa
     2161 ttttatcagt cagagcattg gaaattagat agcgtatttc caaatcgttt atgggacttc
     2221 gctccattgc agaaagatag tctgctttat cgattaagtt ccaatctaca actttttgca
     2281 attcttcctt taaaatgaga tccagccaaa ttctagtact tcggccattt ccttctcgaa
     2341 atgggtgagc gatattcatc tccacatatt tcttgataat ctcttcaaaa gaagtttgtg
     2401 gcatctgatc aatatgctgt aacgaatgtt ctaaatacat aactggtgca aatcgaaaat
     2461 ttccttttgc aatattgacc gatcggattt ttccggcaaa atcgtaaata tccgaaaata
     2521 gaaattcatg aatttcagat aaacctttaa atgtaccaac ttcgatttca tttatttttc
     2581 cacaatcgta caattctttc gctttttgtt tactgagttt ttcttcaacc ttagccaact
     2641 caacttgatt ggttaatccc aatttattct ctaaaatcat gcgaaatctc cttaacttta
     2701 tgacataagt ttatgaaaca ctctcaaccc cattatatcg cgattttgtt tatgtgtcaa
     2761 aaactattaa gtttctgacc ctctacaaaa ttcacaatag atataatcta ttgaaaattt
     2821 aaaagaagac aaaatagagt acagaaaatg ttttctgtac tctataaatg attctgatac
     2881 ttcattgaag tccatggctt taactaatcc atcacgacgt aataatccat ttgaatgttc
     2941 atttagagcg cgttgtgacg gggtgcctgg atccgcaaaa taaatatcaa tatcattttg
     3001 atttcccata gctttccaat ttgaaaattc tttcccacag tcaaacgtaa ttgatttaaa
     3061 cagatgttta ggaaccgttt gaaaccaatg attaattcga ttttcaatgt ccattgcctg
     3121 tctaccggct ggcttcaatg taataatcac ttttgacaaa cgttccacca atgtaataac
     3181 agcacttttg tgttttcgac cgacaatagt gtctccttca agatggccaa actcgttttc
     3241 aaattctgga tgctccttga tgcgctcatg tatactgcgc cgaaaagctt gtttcccacg
     3301 tttttcttgg tgtccatttg gttttcgttt gcctttcata ggcaaatcac gcacattaaa
     3361 tgcgccagtt tgaaacattc gatacagtgt acggactgaa caatcaagtt tcatttccga
     3421 gcggccgaca atcacatccg gtgtccaccc ttgaacaacc ctcttttgaa catattcttt
     3481 ttgttcctct ggtaaagcaa tcgagcgtct accgcagcgt tttttgtttt ctttgtattg
     3541 ttgatagtag ttcagtatcg tagcaccagt tttcaaaaat tgatacacgt tatgaatcgt
     3601 ttgtcacgca cgtcctaagc attgcgcagt tttggcaacg gattgattat ttttgaaata
     3661 agactctatg attaccagtt cgtctgttgt aagatgggtg taggtcattt gtactcactc
     3721 cttaataatt ctttggtcgg aactattttg agtgtagcac aaatgatctt tttagttgtc
     3781 tagcttaatt ttacaatcga cgaaaagaat actatgatga gattagtttg aaatttcatt
     3841 ttgatgagta gatattagga actgattgat agaacgaata agttatattg atattttgta
     3901 tttgcagtat ttgaaaaagt tgaagatgat aaggaaaata aagaaacaac aggaacagtc
     3961 taacttgact attcctgttg ttgaaaagat tatgattaat ttactacttt attttttata
     4021 gacatgctat ttacatactc tatatttcat agttgttatt cataatttca cttccaagta
     4081 tcatcctgta tgaataacca gattggcccg cttgttcttc tgttgctgct tttttagcta
     4141 ggacaacggg atcatctttt ttattatctc ctttaacctc gataatttcc cgacttccat
     4201 ctcttttctt gaccacaaaa tccggaaaat aactacgaac agagttggct tcgggatcga
     4261 tgtattggat gtagaagtct ccttgtttgc tggtaaacat tcctgtaaag aatatctctt
     4321 cgactgtatc attttctaga tattgtaaga aaagttcgcg ctcgggtttt gagtcaaaca
     4381 cataggtatc ggcgtggaag ctctttgttt tataattttc taattgctta tctgaatcca
     4441 ttataaccag ttctggtttg ccactaaatt tgtagtaatt ttttccttga gggaacttca
     4501 agagaacgac ctcgttgtct tctgtaattt gagatttagt tattttgaat ttatactcaa
     4561 atattttagg gattaatatc tcataaacta cttgtttata ggtatttgac cagaagagaa
     4621 tttgctctag tccatcttgc gattcagtta ggattctttg tacctctaga ggactaaagt
     4681 tcaagtatct ggcgatttcg gcgattaaag ttagattgga atattggtat
//
LOCUS       DAZUKC010000139         4708 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000139 DAZUKC010000000
VERSION     DAZUKC010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.145"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(207..1169)
                     /locus_tag="ACG80T_002657"
     CDS             complement(207..1169)
                     /locus_tag="ACG80T_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4842260.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="ACG80T_002658"
     CDS             complement(1171..2610)
                     /locus_tag="ACG80T_002658"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFS4842261.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..>4708
                     /locus_tag="ACG80T_002659"
     CDS             2795..>4708
                     /locus_tag="ACG80T_002659"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFS4842262.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTV"
BASE COUNT         1494 a          810 c          778 g         1626 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttgatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtc
//
LOCUS       DAZUKC010000140         4576 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000140 DAZUKC010000000
VERSION     DAZUKC010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.146"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(15..962)
                     /locus_tag="ACG80T_002660"
     CDS             complement(15..962)
                     /locus_tag="ACG80T_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFS4842263.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(1140..1943)
                     /locus_tag="ACG80T_002661"
     CDS             complement(1140..1943)
                     /locus_tag="ACG80T_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFS4842264.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            2089..3003
                     /locus_tag="ACG80T_002662"
     CDS             2089..3003
                     /locus_tag="ACG80T_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS4842265.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            3051..3521
                     /locus_tag="ACG80T_002663"
     CDS             3051..3521
                     /locus_tag="ACG80T_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFS4842266.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            3606..4214
                     /locus_tag="ACG80T_002664"
     CDS             3606..4214
                     /locus_tag="ACG80T_002664"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFS4842267.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            4348..4548
                     /locus_tag="ACG80T_002665"
     CDS             4348..4548
                     /locus_tag="ACG80T_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4842268.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
BASE COUNT         1503 a          713 c          903 g         1457 t
ORIGIN      
        1 attgttttca gctattaata tcctgcagac aattttactt gattggtttg aagctcatgg
       61 gtggcaaagt agcttttcag attggttaaa aaaatagcca ttaatttctt ttgaaaatca
      121 ggtgtcaaac ctgcaatgtg ggaagtaatc agtacattgt ccattttcca aagaggatga
      181 tcttgtggaa gaggctcttc ctcaaaaacg tctaatgctg caaaggaaag ttgctggtta
      241 tttaaagcct gcaccagatc ttgtgtctta acagaagcac cacggccaac attgatgaat
      301 acagattttg gatcaaagtg ttcaaacata tgctgatcaa agagcccagt tgtttcctcc
      361 gttagaggta agatattgac tacgaaatct acttcaggaa gaacttcctt tagttgattt
      421 aaagaaaaag tcttttccat tccttctgct ggatgaccgg atgtattgat tccatagcag
      481 ttcatgtcta atgctttgca tttttttgca atcatttttc caatatggcc agttccaatg
      541 attagtattt ttttcccttg gatagattgg taatggaccg atgaacctaa ccatttcatg
      601 tcactctgtg ccttttgtgc ttgaaaaaga ccacgagaat acccaaggat gattcctaag
      661 acatgctccg taatcgattc actgtggatg ccactaccat ttgaaagtaa aatatgtttc
      721 tttgaaaaag tctcaagggg tagctgatca acaccagctg agatagattg gacccatttc
      781 aaggtagaag aggctaatag tttttcttgg tagtctgagt tccagcccaa actgatctct
      841 acattttgta gatcctctgg ttgtaattct gttttaaaga tataaccagg cgctatagta
      901 tggatttctt cgataaattc atcacgaaat ggacgatctg cataaatgat tctttctgac
      961 atagttaatt tcccgccttt ctttactcta agtgtagcgc atacaagaag aactttgaag
     1021 cgccttgctt gttttttgag aaagaaatat ttttcaaata aaaaaggggc tgtgacaaaa
     1081 aaatgtcaca acctctaaaa gggtctattg aagtagaaat aacgagttgt ctttatttct
     1141 taattgatcg atatttttgc ccatcctttg acagcttgag caaaaataaa gattcctgca
     1201 aaagcaatga aggcgctagt gtcaaaagaa ggaagaaaga ccatcaagat tgttgcaatt
     1261 atagtaggca atgaaattga atagatcaat gtttttaaac aatcaagaaa agtagctttt
     1321 gttagtcgaa agcgtgcata gagaaaagca gcaatgttgg taaatagcaa agtgatcagc
     1381 agattcagaa aactaggata aatagaaaat ataaatgtca ggacttttat ccaaagtggt
     1441 atagatgctt cacttaaaaa agaacggagc tgcttacctg ttaagttttt taaagattca
     1501 ttggtgtagg gaagttctaa ttggttattg ttcaacaaag cagaagtcgt gcctgtatta
     1561 ggaagagcta tgataagttt gttttttaac atcccgacac taacaaaatt ccccatcaaa
     1621 tcagtagaga tgtcctgttc tgttcgtttt ccctctggat caaatgtgaa gataatggag
     1681 tttgtttggt aaatgaatcc ttctttttct tcggtttgaa gctgcccgtt ttggattgta
     1741 aaatcaggaa tgcgagtagc aatcttttgc ccatccaatt gaatgttgtt taagacttga
     1801 aaaacttgaa aagagattgg caaagccata attatactca aaactaaaag atagacaatg
     1861 gctttccaaa atgggacatt tctcgcctcc tttaggtctt tgaatcgtat aaaggaactg
     1921 ataattaatt gttttgttgt cataagtact cctcatctag tagtgtcatc acttgcaatt
     1981 ctagcgaagt atcaagtgaa atacaagtgc tttaaggaaa cagctgaatg attggtgaaa
     2041 tatcaaaaaa atgttattct tgtctaaaga ctatccaata taaataaaat ggaggaagga
     2101 atagtgccta cacagccaca gagatcatca aaaaaactat ttggagtagt ggttttgaat
     2161 gcggtgatca ctttatcaga atttattgga gggattatgt cagggagttt gtctcttgtt
     2221 tcagatgctt tccataacct tagtgataca ttggcaatta ttttttctta cggggcacaa
     2281 aaaatggccc gaaaagaagc aaataaaaaa cgtacgtatg gctaccagcg tttagaaata
     2341 ttgagtgctt tcatcaactc attcatattg atcatattat ccttgttttt aaccgttgaa
     2401 gcattcaagc ggttcacttc accagaaaag ataaatagtc atttgatgct aacagtagca
     2461 gttattggat tgctcgcgaa tttgttctca gtattattat tacgacaaga agcagatgaa
     2521 agtttgaata taaaatctag ttatttgcat ttgttaagtg atacactgtc ttcggtaagt
     2581 gtgattatcg gagcaatttt gatccgtttt ttcgatatct actggatcga tccagttatt
     2641 acactgatca tttcgatata tatattgata gaagcgataa tggtgatcaa aaaagctgcc
     2701 gctattttga tacaaagtgc acctgctatc gactatgaaa aaatggaaca agaaattgaa
     2761 gcaatcgatg gggtaaagga tgttcatcat gtccatattt ggcagtatag cgaaaagata
     2821 attattttcg acggacatat tgattttgaa gatcagctac taagcgagat tgaaaagata
     2881 tatcctcgta taatttcttt attaaaatta aaatatggta ttacacatgt gacgatccaa
     2941 gcagagacaa aggcaaagga tcaaaaaaaa ttaatttttt taaataaaga tgaaagttat
     3001 tgaaatatag agtatgataa ataagaattt ttaataaggt aggttggctc atgaagcagt
     3061 atcgacagtt aaaaggatat ttagaagcaa aaggatattg ggagatacta gtctatctct
     3121 tttttggtgg tttggctacg attgtaaatt ttgttagttt tgctctcgca cgccaatatt
     3181 ttgatttaag tatggcctta tccaattcta tttcgtggtt ttgttctgta ttgtttgctt
     3241 ttgtaacgaa caaactttgg gtattccatt caaaatcacc gaattttaca catgcactta
     3301 ttgaatgcgg taagttcttc ttctaccgta tcctttctta cggattggac atgggggcaa
     3361 tggtattgtt gatcaatgtt atgaacagca atgagtatgt ggcgaaaatc atcactcaaa
     3421 ttattgttat ccttgcgaac tatattttca gcaagttgtt tatttttaaa gaaactgagg
     3481 tttttgaaga agaagtaggt caaagtgaaa aagacgacta ggcataacaa ttgaagaatt
     3541 tccttctctt tgttactctt tgtaatgtag acattattaa gaaaacattg aggaggaatt
     3601 tttttatgac ttacacatta ccagatctac cttatgcata tgacgcattg gaaccttata
     3661 ttgacgaaga aacgatgcat ctgcatcatg ataagcatca caatacttat gtgacgaatt
     3721 taaattcagc aattgagaaa tacccagaat taggcgaaaa aacaatagaa gaattattat
     3781 ctgatatgga cgctattcca acagatatca agacagctgt acgtaacaat ggtggcggac
     3841 atgctaacca ttcatttttc tgggaaatca tggcaccaaa tgctggtggc gaacctacag
     3901 gagaaataaa agaagcgatt aatgaagctt ttggtgattt ttcttctttt aaagaagaat
     3961 tcaaaaaagc agccgctgga cgatttggtt ctggatgggc ttggcttgta atggaaaatg
     4021 gaaaattagc tattacctct actgcaaatc aagattctcc attgatggaa ggaaagacac
     4081 caattctagg tttggacgtt tgggaacatg cttattattt gaaatataaa aatgtccgac
     4141 cagattatat tgcagcattc tggaatgtta ttaattggga tgaagtaaac aaacgtttcg
     4201 aagcagcaaa ataaccattt cctccgtcat atcagtctta aaaataattt ggaaaataaa
     4261 agagcgaaag cttgctagcg ttttctaaat tgggtataat gttcttgcaa ggttacttgc
     4321 aagaactttt ttggaggata tacatttatg gaacacggaa cggttaaatg gttcaacaat
     4381 gaaaaaggat ttgggtttat tacagtagac ggcggagatg acgtatttgt tcatttctca
     4441 gcaattcaag gagacggctt caagagccta gaagaaggtc aagaagtaga attcacgatt
     4501 gttgaaggtg cacgcggacc tcaagctgct gaagtgacta aattataata atttgttgaa
     4561 taaagacacg acttct
//
LOCUS       DAZUKC010000141         4425 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000141 DAZUKC010000000
VERSION     DAZUKC010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.147"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            690..1658
                     /locus_tag="ACG80T_002666"
     CDS             690..1658
                     /locus_tag="ACG80T_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS4842269.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADARNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="ACG80T_002667"
     CDS             1669..2484
                     /locus_tag="ACG80T_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFS4842270.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKKIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="ACG80T_002668"
     CDS             2552..3262
                     /locus_tag="ACG80T_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4842271.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYVMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="ACG80T_002669"
     CDS             3298..>4425
                     /locus_tag="ACG80T_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFS4842272.1"
                     /translation="MKQVLRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKML
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1488 a          662 c          922 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgcacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaaa aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatgttatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt tttgatttca aggagtagat tttatttatg
     3301 aagcaagtat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatgct tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DAZUKC010000142         4409 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000142 DAZUKC010000000
VERSION     DAZUKC010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.148"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(119..460)
                     /locus_tag="ACG80T_002670"
     CDS             complement(119..460)
                     /locus_tag="ACG80T_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS4842273.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     KKSDQIKRVYFPIAEWEKAGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSASDTKKL
                     NRFIEKLQRKV"
     gene            complement(450..731)
                     /locus_tag="ACG80T_002671"
     CDS             complement(450..731)
                     /locus_tag="ACG80T_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFS4842274.1"
                     /translation="MKVKNPTEKSRIQVGIDKNLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTKDMPVDVLDTDEKLEEWFDEA"
     gene            complement(1102..1341)
                     /locus_tag="ACG80T_002672"
     CDS             complement(1102..1341)
                     /locus_tag="ACG80T_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842275.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEDLLRKTQRKGYPDKILKQIGYYM
                     PEERIEKEFCEVLFDSEQLLVLLTQNKLICQFENN"
     gene            complement(1361..1609)
                     /locus_tag="ACG80T_002673"
     CDS             complement(1361..1609)
                     /locus_tag="ACG80T_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842276.1"
                     /translation="MEGMDFLDHEDLVDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDDTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            complement(1624..2190)
                     /locus_tag="ACG80T_002674"
     CDS             complement(1624..2190)
                     /locus_tag="ACG80T_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842277.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMEKENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGADDCVAYYTTAE"
     gene            complement(2191..2736)
                     /locus_tag="ACG80T_002675"
     CDS             complement(2191..2736)
                     /locus_tag="ACG80T_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842278.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDKSREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            complement(2762..3529)
                     /locus_tag="ACG80T_002676"
     CDS             complement(2762..3529)
                     /locus_tag="ACG80T_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HFS4842279.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
BASE COUNT         1248 a          913 c          619 g         1629 t
ORIGIN      
        1 cttaacgttt tcttaatgtt tataatcacg tatatattta cgtatatttt catatacata
       61 aatacgttta tataaacgta tatcttaata tacatataaa caaaaaagaa aagccttgtt
      121 agacttttct ttgtaatttc tcgataaacc tatttagctt tttcgtatca ctagctgata
      181 acataccaac ctttttgaat gatatgttct ctcggctttt gggaatagcc tttaaagacc
      241 ctacgtcaat ccatgattgc tttttaagtc ctgctttttc ccattctgca ataggaaaat
      301 acaccctctt tatttggtct gattttttct catattgact tgtgattcca aagaattcta
      361 ctctatcttc tttatcacta accaccaaaa taggtcttct tttaccacca tttttatctg
      421 taaatgcaac atagaccgtt actatctcat taggcttcat cgaaccactc ttctaacttt
      481 tcatctgtat ctagaacatc tactggcatg tccttagtta gctgttgcaa tctgattcct
      541 tgcttctcct cttcacttag ctttatctct actggaaact cacctctagc gactacttgt
      601 ttgtaaagaa ttgttattgc tgttgtagga tttaaaccta gctcttccaa tatcatttct
      661 gcgttctctt ttaaattctt gtctattccg acttggattc tgcttttttc agttggattt
      721 ttgaccttca tttcatccac tccttttgag tataaaacac tcattatttc tcaaattgtc
      781 cacatttatg attatattat actcttaata aactacattg caagtaatct aatcaaatta
      841 agatgctata tcatacggta attgaacaaa ttgtcgctaa cccacacata cggaatcgta
      901 atgaatgaaa aaaagacaag cctattcgca gtaggcttgt ctttgtgctt ctcttgtaca
      961 aaatcttcac atttgtaaat taattttagc acctttgtta ggaaagaaca acttaaatga
     1021 gatgctaaag aattaatagt tctcaatcta gcatattcac ttaataccat cttatcttca
     1081 ttcatggttc accacctcat cttaattatt ttcaaattgg caaatcaatt tattttgggt
     1141 taacaaaact aatagctgtt cactatcaaa aagaacctca caaaattctt tttctatcct
     1201 ctcttctggc atgtaataac ctatttgttt caatatctta tctggataac cttttcgttg
     1261 ggtttttctc aataaatctt catcatttcc aacataataa taagaacacc attgcatacc
     1321 cttactttta aattttgtca tgtttctatc ttcctttcac ttattgaccc aaccatgaaa
     1381 aaatatattt tgctaaatct tccttttcta ttccatataa aacattctct aaatccaatt
     1441 tatattcctc gtcaacaagg ctttctgtat cgtcatcata aagaacatat actgcataat
     1501 ttctttcgta aaaggcatta gcaagacttc tgctaatacc taccattcct ttccatgtat
     1561 aaccaaaatc aaccaaatct tcatgatcta gaaaatccat tccttccatg ctgattcaca
     1621 ctcctattct gctgttgtgt aatatgcaac gcaatcatct gcccctacta accacacaca
     1681 ccaatcttca atttcatcat cgcttctctt actcaattcc tctttggtac aaaaaactac
     1741 ttcaccattt tctttttcca tctcataatc ttgtagcatg tattcaaacc aacttctttc
     1801 ggttggaaat tctatctcgt ggcaactctg gcaaataaaa tcaccatatt cattgattcg
     1861 gtagcaacct tcatcttcta caaattcatg attacaacaa acacatctag tacctgttag
     1921 gacttcccca ttaatcgttg ttctaacggt atcttctctt gctacgtgtt cactgctgac
     1981 aagccaatca atgattttct ttgccaattc ttcatcagaa tcggcaaagc ctttaaacgt
     2041 gtttctcttt ccttgaaagt agtatttgca acttgcggta gtttctatgg ttccataact
     2101 tttttggaat aattcaatca tttcaccatc aatcactttt tgacttttcg cttctaaatt
     2161 agggaactgg tcttttcttt tttcaaacac ttatttcacg ctctcttctt ctaggtttat
     2221 tccataattt tctagcaata atctgtatcc cttaataaaa tctttcgtag caaactcttt
     2281 tccgctttca caaaacttgt ttagtcctat ctgttgaccc tcaaaatcaa acaatcgcaa
     2341 tctggtttta tcatctagac taaatccatt ttcatcgaac accatctcta aatgagcata
     2401 gccatcattg ttatctgaaa acttcatcac atcttcaatc gtttcttgat aaggttcata
     2461 gcgcccacca aaccctgttt catcataagc actcactcca gattcatagg taacaaaatt
     2521 gataactcct gtacttctat caaagattcc ttttgctaat tcttctcttg atttatctcg
     2581 taagtcgtat gctttattga ttaaataaat tccttgattc aatacgtcca tcagcatata
     2641 tcgaccatac ccccactgat aatgaatcga cacgcttgct ttcgttttgc tgttaccata
     2701 tttgactgta attaacgctt gtgttctttc gcccatcttg ttttcctctt ttctctaatt
     2761 tttataaaga accattttct tgaaatgagt aatagcttgt gtttttactt gttacgatga
     2821 tatggtctaa caattcaata ccaattgttt ttcctgcgtt tttcaaattc gtagtaaaac
     2881 ttttgtcatt ttcacttgga aattcatatc cgctcggatg attatgtgat actgcgattt
     2941 ttgctgaatt gtttagcaaa gctagttgaa aaatctctct tggtatcgct actgattgat
     3001 taattccacc tttgaaaact tcgctataag caatgacctc attctttgta ttcagacaca
     3061 tcactagcaa agattcacta gcattgtttc caattaagtt agtagcaaag tttgctagac
     3121 caaccgagct tgtgactcta taaaaacttc tatcttcact gatatatttt tgttcgattt
     3181 tcaagacatt cttaatcaat ccttcacctt cattctcggt cttgttttca acttcaatag
     3241 attcaagact gctatttccc atatcagaac ccaacaaagc atttctgaaa tccaatacct
     3301 tttcaaagtt atcagtcata accatttcat aggtgctatt ccattcattc aaatatttct
     3361 ctttgatgag aatgaacgct ctcggtttac ttctgccaat tctgatattt tgtgtatcgt
     3421 gaaaaatata gttatcaagt aaagtaccat cgtacttttc atagttccca ctgatatttg
     3481 ttaacttgtt taccatttct tgattaactt ctaaaattct atctctcatg ttgaacgctt
     3541 ctttctgttt ttgatttttg gtttttcaaa attctttttt gtttggattg ttttagaggg
     3601 aggtggtttt ttcttcatcg ctatttggtt ttgcatttta tgtttcttcc tttcgttcat
     3661 ttatttatct ttttcgcctg tcttgccttg tcgggagtga ttagaatgcg ttcttatctg
     3721 ccggattaat acaagtgttt aagtaaatta ttttcgtaac gcttgcgttt agaaaaaaat
     3781 ttacgccact tgtattaatc caaagatgag ataagctcta aacacttctg acaaggcaag
     3841 acaggcgaaa aagataatgg gcgtaaggaa ttataaatgg caaaaccaaa taaagagcga
     3901 tgaatttcaa tgctttcagg cgtagcttga aagcctaaaa gtcttgaaga atgaaagact
     3961 ttttaccttc aaagcagacc tagtaatctt aaataggcga tggctattta agattacggt
     4021 gttgacccac taaataaaca gaaatggaac cgatcaagca aaatcggact aggcgatggc
     4081 taggacggtt tgcggtggct ttaggaaacg ttcatggtga gatgagcata tctagcaatg
     4141 tcgggctaag ctatgcttag aacggcttgc ggtggcttta ggaaacgttc atggtgagat
     4201 gagcatatct agcaatgtcg ggctaagcta tgcttaggac ggcttgcggt ggctttagga
     4261 aacgttcatg gtgagatgag catatctagc aatgtcgggc taagctatgc ttagaacggc
     4321 ttgcggtggc tttagaaaac ttttatggcg agatgcacta atctagcgat gtcggactag
     4381 gcaatggcta ggacggcttg cggtggctt
//
LOCUS       DAZUKC010000143         4345 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000143 DAZUKC010000000
VERSION     DAZUKC010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.149"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(390..1004)
                     /locus_tag="ACG80T_002677"
     CDS             complement(390..1004)
                     /locus_tag="ACG80T_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS4842280.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1610..1987)
                     /locus_tag="ACG80T_002678"
     CDS             complement(1610..1987)
                     /locus_tag="ACG80T_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842281.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2287..3168)
                     /gene="galU"
                     /locus_tag="ACG80T_002679"
     CDS             complement(2287..3168)
                     /gene="galU"
                     /locus_tag="ACG80T_002679"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFS4842282.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(3211..4230)
                     /locus_tag="ACG80T_002680"
     CDS             complement(3211..4230)
                     /locus_tag="ACG80T_002680"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFS4842283.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         1276 a          921 c          676 g         1472 t
ORIGIN      
        1 gttatatcag gtaaacttca ttgcaataat gataacaaat gtactataat tactttgtaa
       61 agagttttgg ggaagactct ttcatcaacg tgtattaaga gacaagaaag aatccatatg
      121 gattctttct tgtctctttt tccactactt tttatttttc tgacactaca tattcttata
      181 actcatttct cataactgtg aaacaactta taaaaataac aaactttaaa caaaacaagc
      241 ttaaaatcaa agatgatcca tcttaaaata actaaaacaa aaaaagtagt cgttgttaat
      301 cctcattaat aatcgttttc tttttttcaa acgctccatt tttatgtgga acattatttt
      361 tttaaggaac tttgtcaaca tgtggataac tattttccac ttgtgtcctg gactagctgt
      421 tttgctgtaa aatacaaaac ttgctgcagc aaaaagatcg ttgcaagaga ggctgttccg
      481 cagatcgctg gatctagatt tggcaaagtc aagactgcat catgtaaatt ggcgtggttg
      541 tccaaaacca aatcagcaaa ttcaaataat cgcaaaccac tgctatgttg cgatttgata
      601 cttcgtgaca aatcaaatcc agtaacaatg attaatggat gaccattttc tttgatccac
      661 tgagctagtt cgatgattgc cgggtttctt ccttcattcg aaagaagaag aaaaatgtct
      721 tctgatttga cagaaagctg atcaataatt gcctgtcctg caccttctag cgtttcatag
      781 acacctccgg ctggattttc aatcaatata cttggtacaa gatccggggt ttgatcccaa
      841 aattcaaatg ctacaccact tagtgtgcgg ctagcatata tctgcaatct accaccttga
      901 ttaatttgtt tcgcgatcca agtgccagct tgaagcatca gcggctcttc tatccttaat
      961 ttctcttcca attggtgata atagtcaaaa taacataaac tcatttatct ctcccgccta
     1021 gctatacttg acatgaatag aaacagctgc ctaatctaag gaagtacata aaaaaacagt
     1081 agttagacag taacacatgt ggtctttgct gtttcaaaca caaaaaaggc attgaacgaa
     1141 tacgcccaat accttgacat agcaaataaa acaacgtgca gaccatggaa aatatctaca
     1201 taggggtaga attttcaccc tagattaggt acaataaatt aggtcgatgc actgcattga
     1261 ctatggcaaa atgatcggtc agggtgtatg tgtgtgactg gtcattccct gattcttagc
     1321 gccaactaag ggtcagggag ccttttttta ttcagctgtt tctatctgaa tttattgtaa
     1381 gtaatctctt atccaagtgc aagcaagaaa atacaaaaaa acattggtat gatttatata
     1441 tatataaata acataaaaaa aggaatggct tatcccgata caacaagcat tcctttaaaa
     1501 aagattcttt tatataagtg aaaaaagctt ttttccacca attaaataaa tctctcatct
     1561 caatagttta tccattattt tatctttttt tgcgtatacc tactaaatat taccgtcttc
     1621 ttctcgttct tctactttgt tcgagagaat ctgtactaga aaaagtagtt gtatgtctac
     1681 cttttctgcc tatccgagac tttcttttca tatatgggat ataaatgatt agatacagta
     1741 cagcaaaaaa taaagcaaat acgcaagctt cccaactacc taacgccaat tcaaccatca
     1801 aggtagcaac cccaacggat gctgcatacc agataaaaat cagaaacgtt tctttttttc
     1861 tgaccatgcg aaacatgata aatagactaa taagcaatag tggcaacaca aatatccaat
     1921 tgcttccttc catcagccac ttgagtatat caagcagcga ctgtgcaaca gcgactaatt
     1981 ttcccatagt gaacacacac ctttttttat aattaaaaaa ttcgcctttt tattctacta
     2041 tgaatcttct ggcttggctt tcatttctct tatgtaatca ttttctttta tacaacttaa
     2101 aaaaaaatta aaccattagc ataatatttt ctgaatctat tcacgaaagt ctttttctat
     2161 ctttcgtttt ccacgactca actgatctag agtcaaaaaa ctaaaacgac agttgcgcaa
     2221 atgacgcaca agctgccgct ttaataacaa atagcctttc attctcagca ggccacttat
     2281 ttccagttaa ctttctgttc ttagcttttt actcaactgg ataatatatg cacttaatga
     2341 agtcccaatt tccgaatgtt gaagtccata ttcaatattt gtcttcatat atcctaactt
     2401 atcgcctacg tcgtatcttt ttcctttaaa ctctctcgca tagaccgcct ctgtctggtt
     2461 aagagtctga agtgcatccg ttagctgtat ctcgtttcct gctcctggtt cttgagtttc
     2521 taatatctca aatattttag gagtcagtag ataacgtccg ataatggcta aattacttgg
     2581 tgcttttccc atttcaggtt tttctacaaa cccttttact ctgcaacgac ccttttcgct
     2641 atctgtttta ggatcaataa ttccgtattt agatgtttca ttttctggta cacgcatgac
     2701 ggcaacagcg gaagcttgtg tttcttcata atccatcatc aattgttgag tcaatgggat
     2761 atcatctttc atcaaatcat cccccagcat gacgacaaac ggttcatttc ctataaatgc
     2821 ttttgcttga agcacggcat cccccagtcc tcttggatga gattgacgga tgaagtgaag
     2881 attgacttga gtcgtttctt caactaaagc gagtaattct gttttttcct tttcccgcaa
     2941 attgttttcc aattcgatat ttgcatcaaa gtgatcttca atgggtcttt tttcttttcc
     3001 cgttacgatc aagatgtctt cgattcccga atccaatgct tcttccacaa taaactggat
     3061 cgttggttta tccacgattg gaagcatttc ttttgccatg gcttttgttg ccggcaaaaa
     3121 acgtgttcct agcccagcgg caggaatgac tgcttttttg accttcactt gctgttcctc
     3181 ctaaacgacg attaactatt gatcatctat ttaacgaaga gaaaattcat tctccatttt
     3241 tccgtcacgt aacatgattt cttttgctgc ttttttgata tcttctccat tatacaaaac
     3301 attatagatt gtttgagtaa ttggcatctc aacacctagc tgttgagcta gctctactgc
     3361 tgcttttgtc gttgccacac cctcaacgac cattcccatg ttttctagaa cttcttccag
     3421 cttatgtccc tgtcctaaca agttaccggc acgccaattg cgagaatgaa cacttgtaca
     3481 agtaacgatc aagtccccca caccactcaa accgataaat gtcagaggat tggcacccat
     3541 cgcgacacca agacggctga tttctgccaa tcctcgagtc atgattgctg cttttgcatc
     3601 atctccgaat cctaatccat ggatagcgcc ggcaccaatt gcaatgatat ttttcagtgc
     3661 tgcaccagtt tctacgccaa tcacatcatt atttgtataa atcctgaaat aatcattcat
     3721 gaacagtttt tgtacatagg ctgctgcttt ttgatcaata cttgcagctg taattgttgt
     3781 gatatcatgt actgccactt cctcagcatg gctaggacca gataatacaa caattgcctg
     3841 tcttttttct tctggaattt cttcagccaa aacttctgag attcgtttat gcgtatcttg
     3901 ttctagtcct ttacttgcat gtataatgat tggcttagta ttcattttac caataagttc
     3961 ctgtgcgact gaacgaatcg cttttgttgg aacaacaaat aaaatagcat ctgcgttatc
     4021 cactgcttct gccaattctt tatatccgat aatcgattct gggatcgtca aatcaggtaa
     4081 aaaatgctga tttgtatgat acttattgat ttcatcaatc tgttcaggta catttcccca
     4141 aatacggaca tcatgcccgt tttctgccaa tgtttgtgct aaagcagtgc cccaagatcc
     4201 aggaccaaga actgcgattt tttgtttcat ggatattcct ccaaataaat aattgataat
     4261 ttccattata gtatacattt cttaaagttg ggactaattt ccattgtttt taagtgtttg
     4321 tttacttctt ttatcgtttt tactt
//
LOCUS       DAZUKC010000144         4336 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000144 DAZUKC010000000
VERSION     DAZUKC010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.150"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(39..935)
                     /locus_tag="ACG80T_002681"
     CDS             complement(39..935)
                     /locus_tag="ACG80T_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4842284.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(928..1608)
                     /locus_tag="ACG80T_002682"
     CDS             complement(928..1608)
                     /locus_tag="ACG80T_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4842285.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(1609..2391)
                     /locus_tag="ACG80T_002683"
     CDS             complement(1609..2391)
                     /locus_tag="ACG80T_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS4842286.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(2384..3301)
                     /locus_tag="ACG80T_002684"
     CDS             complement(2384..3301)
                     /locus_tag="ACG80T_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS4842287.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(3465..3719)
                     /locus_tag="ACG80T_002685"
     CDS             complement(3465..3719)
                     /locus_tag="ACG80T_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842288.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
BASE COUNT         1272 a          948 c          757 g         1359 t
ORIGIN      
        1 aatacatttt tctactagtt ctcactaatc ttgtataatt aaaccctccg caacttgacg
       61 gagatggaga agatgccccc gtctaatcta gcggtcatct ctcctcccat tccttccacc
      121 aatttttttg acaggtaaag cccgattccg ctagattccg tatttgtgcg gctggtattt
      181 tcggtataaa aacgctggaa gactttttca acagaggttt gcgttcctgg tttgatagga
      241 ttggccactg ttaatcgaat gaatggatcc tcttttgaaa atgtcacctt tgcttgtcca
      301 tctgcataac gcaataaatt ctgtatgata ttttgcagca ctcgcagcaa catgagcgga
      361 tctgctaata cagttgtatt tatcgagtgt tcagcgaacg ctacctcgat atttttctca
      421 gaaaattgtt cataaaaaga gaaaaatact tcttccacct ctttagaaaa agaaagcgta
      481 tggagatcca tttctttttg cttcgtctct attcgagcaa gatcataaaa ctgatccgtc
      541 atactgagta atcttgcaac agattgtttg atgatcgtta aatacttctc ccgtttctcc
      601 gtatcctcta cctgttccag taattgtaca taaccgttaa tagcagtcaa aggagttctg
      661 agatcatgag aaaggccgac gatcgatagt ctgatgtttt cttccatttc ttgaaatgct
      721 tgtccttttt gttcattttc atccaaccat gtattcagtg catctactag gtccattaga
      781 ttcttttctc gaaattcgac tgaaagacga ccaccatatt cttgtcgttc agaaagagtg
      841 gcgattttct tagttaatct cttgatttcc ctttgcagga agaaaataaa acttcccata
      901 aagataacaa cgagaagcaa cagccattta atcaaatcta aaccccactc cccacaccgt
      961 atgaatatac acagcatgtg cctttgctag cttccctcga agattggaaa tatgcatatt
     1021 cactgttttc tcactatcaa aatatggttc ttgccaaact gtttcataaa gatttgctcg
     1081 cgaaaatact tttttcggat gttctaaaaa aagcttcagc agatcgaatt ctcttcctgt
     1141 caaatggaca gccacaccat tcacatttac atccctcgac tctttgtcca gtaagatttc
     1201 cttgtaatag atgcgttgcg acagatcttt tacttggaca gaagtctgat gacgaagttg
     1261 gatgccgatt cgtaacaaaa gttcttcgat atcaaatggt ttcgtcacat agtcatccgc
     1321 tcctgctgcc aacaaggaaa ctttatctct ttgatcgctt tttgctgaaa gaacgatgat
     1381 cggaacattc gatgtctgtc ggatttctgc taacaatgct tcacctgaaa gccctggaag
     1441 catgagatct agaaggatca aatcgacagg atatgtgctt aacaataatt ttccttccgt
     1501 acctgaataa gcatcaagaa gtttgtatct ctgttcaaga acctcctcca gtaaggaatg
     1561 aacagcttca tcgtcttcga taatcaaaat agtttccagc aggaccacct acttaatgtc
     1621 agatttttgg aaaataaaca ttcctagagc tgatgatacc acgattgtta ctagactgac
     1681 cagaatcatt ttcggtgctt gcgaataagt tgcttgttgc gcaaagtccg tatacatgag
     1741 ccacgtttcc gtaaaccact ccgttagaaa ttccaagcct ttgatttttg cgaaaacacc
     1801 gggaatgaag gccgttccca tgatataaag aaaagaaaga agcaaactga cactggtact
     1861 tctagtgatg aatgtcaaag ccactggaac agataatgtc cctaatgtca gcagaagcaa
     1921 tacaggaaat acttttagaa cataatttcc atagtttttg attccctcaa cttcaccaaa
     1981 tccgaaagca agtgtgtaag caagcatggc tagtcccgtg atcaaaacca cgccaagtgt
     2041 tgtgatcaaa agcgtaacga gaagtttcga cacataataa gtcattcgat tccgtcctaa
     2101 agataacgta ttgcgaatcg taccgttttg aaattcttcc ccaatataaa agcacgcaaa
     2161 tgctgcgaaa gccagaagaa tccatgcagc cattttttct accattaaaa tccctgttgc
     2221 accgttgatg actgtatccg ttgcttggtt taattcattg ccgtattttg tatgcatata
     2281 ccattcgttc caagaataaa aaagcggaag tacgaccatc agtcctagtg agacccacat
     2341 tttacggctc ttccatactt ttattttttc agcagttaat agattaaaca tgtggattgc
     2401 ctccagttaa ttgtaagaaa taatcttcca gtttttgccc aactaggtga ataccatcca
     2461 caggaacttt tgcttctacc aatgcttgat tgatggctgc tacttggtca attccttcaa
     2521 aaagctggat aacacggcta gattgtacaa aataatccct ataaccaaga gagtcgagaa
     2581 ccgttactgc ttgttccgtc gccgaagtgt caatttttat aaattgacga gattcttgtg
     2641 ccaattcttc tttcgataat tctttgatca agtgtccttc atgcaaaata ccatagtgag
     2701 tggcgatctg tgacaattca tctagcaagt ggctagaaag caagacggtg atgccccgtt
     2761 cttttaccaa acgttggatg atttctcgca tttctgccat ccctgctgga tccatcccat
     2821 tcgtcggttc atccaaaatc aaaaattctg gattcgtgat cagtgcattt gcaatagcta
     2881 aacgctggcg catacctaaa gagaaattct ttgctttctt tttccctgta ttttccaatc
     2941 gcatcatttt cagcaggtct tcgatttctc tatccgacac accgccgttc gctgcttgga
     3001 tacgtaggtt atctcttgca gttaattctg gataaagagc aggggtttca atcgattgac
     3061 ccatacgtcg tcttgctgcc tgcaaatccg tcgttccaaa taattcgatc gaaccttcat
     3121 caatcgagat caaacccata atagttctca taaaggtaga cttacccgct ccattttccc
     3181 caatcaatcc atagatcatt ccacgtttga tttcgattga tactttatct aatgctttgg
     3241 cagcaccgta ttttttggtt ataccagttg ctttcaagac cgtttcattt ccaaaatcca
     3301 tacttatcct tctttcttat tgatgagtga agtatagttg acgggtataa agaaagcagc
     3361 cagaaatgta taaagaaagt gtaaattttc caattgaatg tcaaaaaaaa caatcggaca
     3421 atacgtgtct ttcctattat caggcagttt gttatacgct tgtatcagtt tcctgattct
     3481 ttcttttcga ccaaagtcgt ttctctgtat tcctcttcac tgaaaatttt tgcttctttc
     3541 agatcaagct tgagtaattt taccagaccg gttataagga taactatagc aagactccct
     3601 cccagaaaaa ttagactgtc tcgcagacgt ggttgcgttg tcttccaatc aaagagcgca
     3661 ccaaacagtg ctaatccaag tggactggcc accatggtaa tcgttgttat cgttgacata
     3721 agtcgtcctt gtatatgcgt tggaacttct cgctgaagcc agaccatata aaaagcaaaa
     3781 gaaaacatta atccttaaaa acggacttat gaaaaataat acatgccgtt tgggatttac
     3841 aattagctac caaataagag gttatgacaa aagacttttc acaatccctt cttccgaata
     3901 aacggtgttc tcctgccaac tcctcggtca caagtcacaa tatttatcaa acatgagaag
     3961 caatttttgt aaattccttc ttatttcttg aagctgacca cttctgtcac aacctcagaa
     4021 gcggataaat aatattgttt gatgatactt tatctttttc ccgtttgttt atagatcgaa
     4081 tgatattata gaccaagaat cctacctatt aacatagcta aaaacacaaa aaaggcactt
     4141 tccaaattgt ggaaagtgcc agaaaagtta ggattaaggt aactgcgctc agcattgctt
     4201 cgcccagtac gattctcgta actcgtgaac tcgaacgata atcgcaatta acgttttgag
     4261 aactgtgaag ctttacgggc tttcttaaga cctggttttt tcttcgtatc attgaataag
     4321 tttgaaaaac cttgat
//
LOCUS       DAZUKC010000145         4293 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000145 DAZUKC010000000
VERSION     DAZUKC010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.151"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..790
                     /locus_tag="ACG80T_002686"
     CDS             <1..790
                     /locus_tag="ACG80T_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS4842289.1"
                     /translation="CGYSKTEPRHSFGPAIREQYLIHIVLEGEGYYSINDQKYFLKKG
                     QGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYYLKEIGVGKNHLTFKVENLNSFK
                     GLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSASYLNEGIITKKMNPFVRQTIEII
                     RENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIKEYISEIKISIANDLLSSTEYS
                     IQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLGLGIINGE"
     gene            887..3136
                     /locus_tag="ACG80T_002687"
     CDS             887..3136
                     /locus_tag="ACG80T_002687"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFS4842290.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3139..>4293
                     /locus_tag="ACG80T_002688"
     CDS             3139..>4293
                     /locus_tag="ACG80T_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4842291.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTR"
BASE COUNT         1501 a          610 c          790 g         1392 t
ORIGIN      
        1 ttgtgggtat tcaaaaacag aacctagaca tagttttgga ccagctattc gagaacaata
       61 cttaatacac atagtgctag aaggtgaagg ctattattca attaatgatc aaaaatattt
      121 tctgaagaaa ggccaaggat ttgttatccc accaggagta tctacttttt atcaagcaga
      181 tgaaaaaaat ccgtggtcat atgtatggtt gggtttaagt ggcaatcttg tagaatacta
      241 tctaaaagaa attggtgttg gaaagaatca tcttaccttt aaagtcgaaa atttaaattc
      301 atttaaagga ttaatttttg aaactttatc gtatgatcag gacaatctag tcagtgaatt
      361 gtccagacaa aaacaagttt ataaattttt agaactcctc ttaaattcag ctagttatct
      421 gaacgaaggg ataattacta aaaagatgaa tccttttgtt cgtcaaacaa ttgaaataat
      481 acgtgagaat gcgtatcaaa aattatctgt taaagatatt gctgtcagtc tatcgataca
      541 cgaaaattat ttatcacgat tgtttaagaa tgatatggga atatcaataa aagagtatat
      601 tagtgaaatc aaaatttcta ttgcaaacga tctgttatca tcaactgaat attcaatcca
      661 gcaaatcagt gacaagatag gattctcaac agtacaatct ttttctaaag cctttaagaa
      721 agcaaatggt gtttcaccgg gagtatatcg aaaacgtaga ttaggtctag gaatcattaa
      781 tggtgaataa actaaacatt tgattttgat aaatattcgt tactttaaac gcttactatt
      841 tatgtgagcg ttttttataa tgcaataaag aaacggagga gagcttatgt ataaggataa
      901 tgaaatgaat attatttatc gatcagaaaa ccaacaattt catttgttta atcaggagat
      961 aagttacatt tttaagatat cggaagatgg taaattattg catttatatt atggtgagaa
     1021 cttaccagat aatgactata gtcatctaat tgagatgcat catcggccta tgacaaccta
     1081 tagaaatgaa aacgatttgc tatattcgtt agagcactta aaacaagaat ttcctgaata
     1141 tggtacaaca gattttagac atcctgctat ttcattacta cagaaaaatg gctctcgctt
     1201 atcagatttt gtctatcaag ggtacgaagt ggaagaagga aaacctaaac tagaaggttt
     1261 acctgctacc tatgttgata ataatgatga aagcaaaaca ttgaaagttt atattttaga
     1321 ttcagttaca aatgttcaag tagaattatt atatacaatt tatagagatt atcctgttat
     1381 tactcgtagc gcgcgagtta tgaataaagg aaaagatatt caaataatcg agaatattag
     1441 cagtttatca ttggatttac ccgatgcaaa ttatgaatgg caacaattat ctggtgcttg
     1501 gggtagagaa agaactatta agtcgcgtac tttacaacaa ggaattcaat caatcgaatc
     1561 tactcgaggt atttcaagcc atcagcataa cccatttgtt actttaaaaa gaaaaaatac
     1621 tgatgaaaat caaggagaag ctattggtgc agcactagtt tactcaggaa acttcttaat
     1681 tcaagcagaa gtagatacat gggatgttac tagactacaa gttggcatca atccatttgg
     1741 atttaattgg aaactcaatc caggagaaat atttacagca ccagaagcga ttctggttta
     1801 ttcaagtaag ggtttaaatg ccatgagtca aacttttcat caactattta gaaaacgctt
     1861 agctcgtggt aagtggcgag aaaaagaccg tcctgtctta attaataatt gggaagcaac
     1921 atattttgat tttgacgaag aaaagttggt aaatattgct aaaaaagcat ttgatgttgg
     1981 tattgaatta tttgtattag atgatggctg gtttggagaa agagaaaacg attgtgcagg
     2041 attaggagat tggcatgtaa atcctaaaag actacctaga ggaatcggca gcctttcaga
     2101 aaaaattcgt agttttggta tgaaatttgg tttatggttt gaacctgaaa tggtgaataa
     2161 ggatagtgag ttatatcgca cacatccgaa ttggattatt tctacaccta atcgaaaacc
     2221 taatcatggt aggaaccaat atgtattaaa ttttgggtta gatgaggttg ttgaaaatat
     2281 tttcgatcaa atgtgtaaaa tcattgatga atcaaactta gattacatta agtgggatat
     2341 gaatcgtcca ttgactgatg tatttgacag tcacttgtca gcagatcaac aaggagaagt
     2401 attccacaga tatgtactag gcgtatatcg cttatatgaa aaacttataa caaaatatcc
     2461 taacatacta tttgagtctt gttcatcagg tggtgggcgc tttgatcctg gaatgcttca
     2521 ctatgcacct caagcttggg ctagtgatga ttcagatgca attgaaaggt taaaaattca
     2581 atatggaact agtatgctgt atcctctttc gtctattggt gctcatgtat caattgttcc
     2641 aaatcatcaa acaaaccgta taacacctat aaaaactaga ggaaatgtag ctttttttgg
     2701 tgcatttgga tatgagcttg acttaaatga attaagtgaa ggcgatttga ggattgtaaa
     2761 agaacaaatt gccttttata aaaaatatag aaccataatt cataatggta ctttttatag
     2821 actattatca ccattcgatg atgataatca aaccgcgtgg atggttgtta gtcatgataa
     2881 gaaaacggct attgttgctc attataaaac attaaatgag gtaaatgctc cttaccgccg
     2941 actgaaatta aaagggttag ataatgacgg actttataat gtaggacaaa atagtttgcg
     3001 atctggaatg gaattgatga gagcaggact agtctgttca gatgcatcta gtggacaaat
     3061 aaatgatcag aatcgacgcc cagaaacatt tgattttgat tccaagattt gggttataac
     3121 acaggaggaa aagtagagat gaataaccaa aaaagcttac ttcaaaaagg agcaatctta
     3181 agtatttcac taatgttaac aagctctcaa gcaattaatg gtgttattcc acaaatgaaa
     3241 cagtctttgg ggatatcaca aagtcaaagt gaacttttag gaactgcacc aagtataact
     3301 gttattttgt ttatcttatt atctagttac tttgccaaaa gaataggaat gaaaaagaca
     3361 ataattttag gtttattatt agctggtata ggaggaatta ttccagtatt tacagctacc
     3421 ttccaaaccg ttcttattag tcgagtaata ttaggtgccg gtttaggagt atataattca
     3481 ttggctgtga catatatcag ctctttatac tctggcaata ctagagcaac gctattaggt
     3541 attagaaatt caatggaagc tttaggtcag acaatcctta tttttcttgc tggagtttta
     3601 gtgaacatta attggacggc ttcgttttta gtctatgcaa ttgcgtttcc tgtagctctc
     3661 ttattcgcgc taaaagttcc tgaaattaga gatgaaaata gtgacatacc tgaaaatcat
     3721 gtaaaagaaa agatgaatcc aatggttttt gccctagttc tatttgcaat cttactcgta
     3781 atgaatagta ttgctatttc tgtacgattt gcatcaattg caactgaaat taaaggggta
     3841 aactttaatg ctagtaacta tttagctcta atgcctatct taggaatact agcaggattt
     3901 atgtttggac caataaataa gtggatagga aaaggaaccc tatacttggg aattatattc
     3961 tttataattt caaatttgtt aattgctgtg tcaaacggta atatgacgtt cctgctaacg
     4021 ggtttatttt tatcaagtat aacaggatca tggtgcttcc catttatctt tagtaactta
     4081 gataaagtca ctacaaagaa tacaattaat tttgctactt cattgatttt tattggctgt
     4141 aatattggta attttatagc tcctattgct atgcaattag cacaatttct aacaagaagt
     4201 acaaatttga ctgctccatt ttttgtattc gcaggaatat ttattgtgat cctatttgta
     4261 acattttttg cgacaaataa aaaaaatact aga
//
LOCUS       DAZUKC010000146         4098 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000146 DAZUKC010000000
VERSION     DAZUKC010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.152"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1037
                     /locus_tag="ACG80T_002689"
     CDS             <1..1037
                     /locus_tag="ACG80T_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HFS4842292.1"
                     /translation="LMKKSFRTVTLDNIQQGIKHLIQLISSALILWLGSYFVMDNRIS
                     LGQLITYNALLVFLTDPLQNIINLQVKMQTAQVANNRLNEIFAIDSEKSNQSFEYMLS
                     EQLFQQDITVKNVSFSYSMKAPTLNEISCTIPSHSKTAIVGVSGSGKSTLAKLFVRFY
                     EASEGSIHYGNINHLDIPFQILRDRVTYLPQESFFFSGTIIENLIFGLDSPPSFEQII
                     EVCEIVQLNEFINQQPLRYETLLEEGGNNLSGGQRQRLALARALLKNPDILILDEATS
                     GLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAKACDQILVLHEGHLVEKGTHDELRFA
                     DGIYQKLWEI"
     gene            1006..2421
                     /locus_tag="ACG80T_002690"
     CDS             1006..2421
                     /locus_tag="ACG80T_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFS4842293.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            2454..3488
                     /gene="sppA"
                     /locus_tag="ACG80T_002691"
     CDS             2454..3488
                     /gene="sppA"
                     /locus_tag="ACG80T_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFS4842294.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            3490..4011
                     /locus_tag="ACG80T_002692"
     CDS             3490..4011
                     /locus_tag="ACG80T_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFS4842295.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
BASE COUNT         1526 a          566 c          785 g         1221 t
ORIGIN      
        1 aattaatgaa aaaatcattt cgtacggtta cgcttgataa tattcaacaa ggaattaaac
       61 atttgattca attaattagc agtgcattaa ttttatggct aggttcttat tttgttatgg
      121 ataatagaat tagtctaggt caacttatca catacaatgc tttactagta tttttgacag
      181 atcctttaca aaacattatt aatttacaag tgaaaatgca aacggctcaa gttgcaaata
      241 atcgtttaaa tgaaattttc gcgattgatt cagaaaaatc gaaccagtct tttgaatata
      301 tgctttctga acaattattt caacaagata tcactgtaaa aaacgtttca ttctcgtata
      361 gcatgaaggc cccaacgttg aacgaaattt catgtacaat tccttcgcac agtaaaacgg
      421 ctatagttgg agttagtggt tctggaaaat caactcttgc aaagttattt gttcgttttt
      481 atgaagcatc agaaggatcg atccattatg gaaacattaa tcacttagac attccttttc
      541 agatattacg cgatcgagtg acatatttgc ctcaagagtc atttttcttt agtggaacaa
      601 tcattgagaa tcttatattc ggtttagata gcccaccaag tttcgaacaa ataatagaag
      661 tttgtgagat agtccaacta aatgaattta ttaatcaaca gccattacgt tacgagacat
      721 tgttggaaga aggtggaaat aatctttcag gagggcaacg acaacgatta gccttagcac
      781 gggctttatt aaaaaatccg gacatattaa ttttagatga agcaactagt ggattagata
      841 ccttgttaga acatgcaatt atgaattatc tcctcaattt aaaagaaaaa acgattattt
      901 ttattgcgca tcatttacca atagcaaaag catgtgatca aattttagtt ctccatgaag
      961 ggcatttggt agaaaaagga acgcacgatg aacttcgttt tgccgatggt atctatcaaa
     1021 aattatggga aatctaggag agggaaacaa tttatgcaga ataataattg gttcgaacat
     1081 tctagtatct atactaaaca acatcatagc ttctaccgtt gggtgttata ccctgtaatt
     1141 ctttttttat gtattattgg attgttttta acctttgcta aaaaagaggt tgttattcgg
     1201 acaacggctc aattgaaccc cgaaaaaata gagaagttac aagtgccatt agaagctaaa
     1261 ataatggaaa acttcctaaa agaaaatcaa tttgttcata aggacgatgt gttggttaga
     1321 ttggattgtt ctttaataga gaatgaaaag gctcagatag aacaagaaaa ccaacgtatt
     1381 actcaacaaa taaagatggc tcagctattt attgaaagta taagtaaagg aaaaaatttg
     1441 ttttcaacgg atgacagttt tggctacagt aatcaattaa agagcatgtt gtcagaaaaa
     1501 gaatcactcc gctacgcttt gaagcaaagt gaattaaatg atcaaaagca attagaagta
     1561 tacgaaaaga caaaaagaca actagaaaaa caaattgaga gttcagatag taaattacaa
     1621 gaatggcaac aagtacaggt agcttggagt aataatcaat cattaaaaga tttttcaaaa
     1681 gaaatgatgg caaactatga gaattggcaa gaacaactaa ataatgtttc tgatgatcaa
     1741 aaaaatcaag tgaaactgac aatttcagca agcataaatg aacaaattga gcaactaaaa
     1801 aaagaagtag aacagtatca gtcagaaaaa gctaaattag ttaaaccaac tacttctgag
     1861 aatgacagaa ttagtcaaac ggaaaaagga aagcaagagc tagaacaagc aataacaact
     1921 gtaaaacaaa cagttacaga gctaaatgaa aaacaagaga agaaccagtc agttatcaaa
     1981 agtttggacg atcaactttc aaaaggaata ctaaaagctc ctgtatctgg aacggttcat
     2041 ttaaatgaag aaacaaaggg acaagcagaa ctagccaaag gaacagtgct tgcagaaatt
     2101 tatccgatcc acgaaaaatc aaaaatgaaa tttacagcac ttttacctgc gaatgaaagt
     2161 acctatatta aagaagggat gaaggttcat tttaaattgg atcaaaaagg tggggctccg
     2221 attacgatcg acggaacttt agatgaaatt tcagaaaatt ctactgccac tgagaaaggt
     2281 gttttttatg cagtgaaagg aacgttaaaa acaactcaat cgtttccgtt tcgttatgga
     2341 ttaactggag agttgtcttt aattgtagga gaaaaaacat attggcaaca aattgtcgat
     2401 gtgtttctaa ataaaaaata attattttta ctaagaatat aggaggagtt tatatgaata
     2461 aaagaagatg gattgctgtg ggagtagcgt gcggattatt gttactatcc attatcgtcg
     2521 cgctaattcc aggaaaagat aaagaggaag catcaaatac aaccctaaca ggaataaata
     2581 agatttttta cggatctaac gaaatcacag aagaaacgtt agaagaaggc gcatcaaata
     2641 aaaagattgt caaactatcc gtcaatggag ttattgcaga tacaggtgaa agcaatcttt
     2701 ttagtaggga acaatataac catcagaatt ttttaacaca gctaaagaaa attcaagaag
     2761 acaaagcggt taagggagtg ttattagaag taaattcacc tgggggagga atatatgaaa
     2821 gtgctgagat tgctaaagaa atggcaaaaa ttaaaaagct agatattcct atctatactg
     2881 catttaaaaa tacagcggca agtggagggt actacatttc tgctggttca gataaaatat
     2941 ttgctacgga agaaacaaca actggatcaa ttggtgtcat tatttctggt ttaaattatt
     3001 ctggcttatt agaaaaactt ggcgtaactg atgcgacgta taaaagtggt gcattaaaag
     3061 atatgatgtc acctcaacac aaaccttcag aggaagagaa taaagttata caggaatttg
     3121 tgatgagtgc ttatgatcgt tttgtcaatg ttgtagcaaa aggaagaaat atggatacta
     3181 atgcagtgaa agaacttgcg gatggaagaa tttatgatgg taaccaagca gtagaaaatg
     3241 ggttggtgga tcaaattggt tactctgaag atgcattaga ttccctaaaa aaagagaaga
     3301 aattaactga tgcgacggtt atagaatata aaaatgacac aactggcttt gcttcttctt
     3361 ggttaggtaa taagattgct gaatggcaag ggctaaaggc aactacatca gatcgtgtaa
     3421 tttcagtttt tgaaaaactt ggattttctg atactccaaa accaatgtat tactatggag
     3481 ggctataaaa tgaataagaa gagtggcatt gaaactttgc ctaattatgt atttgcggga
     3541 ttttggttac gattttttgc ttttcttatt gatttaattt gtattagcat catcacccat
     3601 gctactatag gtctctttta caaaatcatg ggactggaat ctgacaaatc cattattact
     3661 ttttatggta tagcgtcatt agctatttat ttaacctatt ttgtactttt gactaagctg
     3721 aattcaggac aaacaattgg gaaaatgatt tttgggataa gagtagtgag ctttgaggaa
     3781 aaagagctaa gttggcaaac tatctttatt cgtgagtgta tatgtaggtt tatattaaaa
     3841 aattttgtat ttcaaattgg gtatttggtg gctgctttca cgcccaagaa acaacatatt
     3901 ggggattatt tttctaacac gtcagtggtt gttattcgtt cattagaagc tattaactat
     3961 ggtcaaaaaa tttctaattc taaaacactt aatctggaga gaattaaatg aataagagaa
     4021 aaacagagtt tattttgatt ttaattagtg gaataacagg gattttatgt agtcttttag
     4081 cattgatggg tggtatgt
//
LOCUS       DAZUKC010000147         4079 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000147 DAZUKC010000000
VERSION     DAZUKC010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.153"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            56..1357
                     /locus_tag="ACG80T_002693"
     CDS             56..1357
                     /locus_tag="ACG80T_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4842296.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEKAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNATLLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPMIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1384..1644
                     /locus_tag="ACG80T_002694"
     CDS             1384..1644
                     /locus_tag="ACG80T_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFS4842297.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2643..3878)
                     /locus_tag="ACG80T_002695"
     CDS             complement(2643..3878)
                     /locus_tag="ACG80T_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4842298.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1159 a          665 c          851 g         1404 t
ORIGIN      
        1 ctttgcgttg tcatgcaatg aatggttcat attttattga aaaggagtgg ttttagtgga
       61 aaagctgatg aaatgggtcg aagagaaact tgtcccacca atggcaaaaa ttggtacaca
      121 gcgtcattta ttagcaattc gtaatggggt tgtttcaaca ttgtcattga ttttgattgg
      181 gacatttttt atggtcttta tcaatttacc gtttcctgga tggaatgaat ttattgcccc
      241 ttattcggca acgattgttt taccttttag gatgacgatg ggattgatgg ctatttatgc
      301 aacttttgtt atgggttcgg atctagctaa atcatatgga ttagatagtg tgacaggtgg
      361 tatattatct ttaggaacgt tctttatgtt acaagttcct gtaaacgttt taactcctga
      421 aaaggcacct cttggctggg tcttacctat gagttctttg ggagcatctg ggatgtttgc
      481 ggggatctta tctatgattt ttgcagtgga agtatatcgt ttctttaaaa atcgaaatat
      541 tactatcaaa atgccagaac aagtgccacc tgctgttgcc cgtagttttg aggcattgat
      601 tccaggtgca gtcattttaa caattacttg gctgattcga agcgtcgcgg gctttgatgt
      661 aaacgctacc ttattatcgt tattcgagcc tttaaccaat attttaggga ataatttgct
      721 tggtgtttta ctaccaatgt ttttgatcca tttgttgtgg tcatttggta tccatggcat
      781 gagtattgta ggggctgttg ttcgtccaat gtggctgatc atgttggatg agaatgcaaa
      841 ggcattggca gatggtacag ctgccaccca attaccctat atcgctcctg aacagtttta
      901 tcaatggacg gtaacaattg gtggtgcagg agcaacgatc gtggtatctg ttttgtttat
      961 attcttctgt aaatcaaaat tcttgaaaga ggttggtcgt ttttctatta ttcctggtat
     1021 cttcaatatc aatgagccca tgatttttgg agctcctatg atgttgaatc cttacatgtt
     1081 catcccgttc aacttagttc ctttagtttt gactattatc agttattttg cagtcaaatt
     1141 agaaatggtc aatagattta ctgttttgcc aacttggaca ttaccagctc caatcggcgg
     1201 ttatctttca gcaggaaatg actggcgtgt tgttatcttg atcatgatca acacattgat
     1261 agcatttatt atctattatc catttgtaaa agcttatgat aaaaagatgc tgtcagatga
     1321 acaaagtcgt ggagaaaacg aagtccaaac tgtctaagtt tggtttttag aaggtgggaa
     1381 acaatgtatt ggctgatttt attttttgtt tttatctttt tattaacggc tagtcacctt
     1441 atattgaata tgctagctac atatcatatt caaatcaatc gatggatttg ggcactggca
     1501 tcatttttga tcgtgattct tccaaagatc attgtaccgc atatgaatgt tttgttttca
     1561 tgggggacat atgtactttg tggaattttt gctatcaatt ttatgatcga gcagcataga
     1621 tggtttgtca catcgaagct ttaaatgttc taaaagtggt tgtgacagaa gtggttagct
     1681 tcaagaaata agaagaaatt tacgaaaatt gcttcttaaa tttttgtgaa atgttggctt
     1741 atttttcgtt tggttgaact tattaccgca gatgcaagct tttgaccacc gtttattcgg
     1801 aatatgggac tgtgacaagg cttttgtcac agcccttttt tacataaaga ttattattgt
     1861 tttaactgtt ttggcagaaa gaaggaggca aaaatgaaaa ttttatcagc aatggattaa
     1921 gaattagtcg atctactacc aaagtgatca aggcaaatat agatttggga gcttacaacc
     1981 atatagtagg ggctgcagta tggtgtatga atggtaatat gtatgatgag agtgtttatt
     2041 caaatcatga aacgtgtata gaaataattg cgctgggaat agcaatcagt catggtgaac
     2101 gtgactttta gtttatcgtt gctgacgaca tagaacattc tgacatgatc tactcagcat
     2161 gtggggattg ctggcagttt atataggagt atgcatcgga ttgcgagatc attgtctcaa
     2221 cagaagaggg aggttgatta tcaaagataa agtggtaaat cctacatttg atttagtaat
     2281 tgttgaaggg actagcagca caaatctcta caaagcgatg cttctagatc tatatgacac
     2341 attttaagaa aatgtatttg cctaattatt atggtctttc ttttgaagaa acgttgagat
     2401 gatccaacca gagaaaggtg tcaaagaatt tgactaagaa taaatggcag acgctaccta
     2461 tgctcatttg gattgcgtca gatcttttat tttaaggatg attttgtgtt ttttagacac
     2521 cgtaatttaa gtaactctaa cttttcaatc cactatttac tttttttgta ttttcactgt
     2581 ctgggtctcg aatagcgaaa ttactgatga gtctctatta aaaaagcttt ttctacttga
     2641 aactatattg ggtggttgct caggaaggtt tcgatttctt gaattgcttg ttgtggacca
     2701 tttattggaa aaacggcttg cagcgtgcgt gcctgctgac gaatcttggg tgaagaaact
     2761 tcgcgcagag caatctgcag ttttctttca gtcactggtt ttgtgagggc gattgcgttt
     2821 ccgtatttta cacaatagtc aatattacag ctttgctcaa attgcatccc cacaccgata
     2881 aacggtttgc cggattcaca ggcagtttgg acggtccctt ccccgccgtg gataaaagaa
     2941 aaatcaatga attcatgcaa aagatgacta gacagatatt ctgttattaa aagatcttct
     3001 gagtcagatt gaatgtcatc aagcagatag tcaataggtg caataaccgc tagatccggt
     3061 tgtttttgta agaagaacaa aattttgcgt aacatacgcg gattgcctga actacccatt
     3121 gcgaaataga taatacgttt tctttcttgt tgctttcgct gtataaaagt tagcacttct
     3181 tcaggcaagg aagtactgag cttagcaaaa attggaccaa cgattcgata attttccggc
     3241 agctgttctt cttccaaaaa tagtttaggc gttgtgatga gatttagatc agcatccatc
     3301 atttgtaaac ttgtttttgg taaaggaagc atttcttttt gtgcagcgtg tttgaaggca
     3361 ttggggatcc atcgggtatt tactatacgc ttttttgtcc agtgccaaag gggattgctt
     3421 aaagggccga agaaacgtaa ctcgtggggc atcagcgcat gctctgattg aaaaaaaggt
     3481 tcactcaaag catagggttt aacatatact aaaggaatct ttttattgcg ggccgataaa
     3541 aaaattgtca cattcgaacc tgtcacaatc agtcgaggct caaacttagc aatcagactc
     3601 tgttcactag tgatacgaga ggcaaccatt tctttggtaa aagggttttt aagtgtacgc
     3661 aactggtcaa attttatgat ttgttcttct tgttttttcg taaggatcgg cgctagtaaa
     3721 tgatactcaa agccactttc tgtgatcaaa ggagcaaaag ttttagaaaa gccaaagaat
     3781 acacagtaat aacggtcttt catagctttg gcaacctcaa tcattcgtgt tgtctcagct
     3841 aaattgaata ctgcaggagc aaaaagaatt gtcttcatga aatcctcctt attggaatga
     3901 gtctatccct tcgtttctta ctagtatact aaaaaacaca gcgttcgagt agcctatgta
     3961 tattttgaat attaaactgt ctctcgctgt tttcacaact tctaaattta aattttgtaa
     4021 tcaagttgaa actccaattt tcaaaaaaac acgcaacaat tttttgatcg atttatatg
//
LOCUS       DAZUKC010000148         3864 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000148 DAZUKC010000000
VERSION     DAZUKC010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.154"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..91)
                     /locus_tag="ACG80T_002696"
     CDS             complement(<1..91)
                     /locus_tag="ACG80T_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFS4842299.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQV"
     gene            complement(221..805)
                     /locus_tag="ACG80T_002697"
     CDS             complement(221..805)
                     /locus_tag="ACG80T_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4842300.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(1136..1369)
                     /locus_tag="ACG80T_002698"
     CDS             complement(1136..1369)
                     /locus_tag="ACG80T_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFS4842301.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(1384..2334)
                     /locus_tag="ACG80T_002699"
     CDS             complement(1384..2334)
                     /locus_tag="ACG80T_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFS4842302.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(2331..3173)
                     /locus_tag="ACG80T_002700"
     CDS             complement(2331..3173)
                     /locus_tag="ACG80T_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFS4842303.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(3175..>3864)
                     /locus_tag="ACG80T_002701"
     CDS             complement(3175..>3864)
                     /locus_tag="ACG80T_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFS4842304.1"
                     /translation="YNDFLALNEISLKIQEGALTGIIGPNGAGKSTLLKAMLNIIPHQ
                     GEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKVFQRIKNAEWQ
                     RVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEPFVGIDLVSER
                     IIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVESVFNEENLKKA
                     YGDTIFIGEGE"
BASE COUNT         1286 a          715 c          472 g         1391 t
ORIGIN      
        1 ccacttgaaa tatatttccc tttcctaaaa aaataatgac cggaaaaaaa atatttaaag
       61 taatcaaggt taacggattg atttttgaca ctctttcacc tccaaaaaat aagatcgcac
      121 tgttatctta agctgataaa aatgcaccgt tattcagata acagtgcgat cttatcaaaa
      181 agcataactg agaacaattc tcaatgcaag ctttttttaa ttaactcagt aattcaatcc
      241 acccaggaac agaaaattga tacaatcttc tggttaatag catagtcttt tcttttgaat
      301 agctatgctt caatatatca tagaatgtag cgtagaatga gtaaatgtag aagtggattt
      361 cttcttcatt aaagactaaa ttttgattga tcattccgat agtctttaac ttatctagac
      421 aacagactgt ctttttcgtc acaaatgatg taattttttc aacaatatct tgatatttgg
      481 ttccagctga ttggaataaa atcaaatcga atatacgttt attctcaaaa atataattaa
      541 taaatggatc taattcattg aagttctctc tccaactttt ctctatatct atattttcta
      601 agtttaagag gtatttggta aactcttcat tatacatatt aataagcgca ttaatatgcg
      661 gctgaataat ttcttcaaat aacgcttcct tagaagtaaa atgtttataa aaagcacccg
      721 tagttacgcc tgcggtcagg cacagttgcc ttatcttagt ttgctgaaat ccttcttttt
      781 caaaatggct tagtccactt tctattaact tttggtgagt tataatataa tctctggttt
      841 ctatgggtac ttcctcctaa taagatgaat taactttatt atcttacaca ggacttgaag
      901 tttcaatcgt taacgtttat tactgaactc attccttaga gctaaactaa tgagaaagag
      961 cagaataact gatacaaaaa gcaatcctac aagaattaat agactcatct aaatcattca
     1021 ccttttctaa aataatccga gaaagattta aaaaaattat atccagctat taggataagt
     1081 cctatcataa tcactccaat agccattttc ttatcctccc taaaaataaa gtcttttact
     1141 taaaaccttt tttgtaatcg tcaataaatt ttttcccttt atcagttaat tgaaatccta
     1201 tatctaaata gtcctcatat ctaagtttag ccagcatctc acttacagat gctggactaa
     1261 cacttagaac ttttgctaaa tcattatttt ttaatttctt ttttcttttt gataattcct
     1321 gaattgctag taaataaatt agtatagtag tcgttttaat ttgtaccata cctaccctcc
     1381 aaattatttc gaaaggcctt cagcaatttt atccaggttc catttcatca tcgcatagta
     1441 actatcacca tcttgtcctt tttctgcaat tgaatcagta aaaatcgttg aatagatagg
     1501 aatattggta tcttttgata ctgttttcat tggtctatca tccacactgc tttctacgaa
     1561 taaggaggga acttttgttg tgcgtaattt ttcaactaag tgttttattt gatctggtgt
     1621 tccttcttct tcagtgttga tttcccaaat gtaagcagaa ggcacattat acgctttcga
     1681 gaaatattta aagcatcctt cacttgtaac aatcattttt ttatcattcg gaattgaagc
     1741 aaatttagat ttagcttctt tgtctagaga atccaatttt tcaatatact tatctagatt
     1801 ttctttatag aattttttat tatcaggatc tttttccgct aactgttttt caatattttt
     1861 agcgtaaata ataccgtttt ctaaatttaa ccaagcatga ggatcttcct tccctttctc
     1921 actctgaccc tctaagtaaa taacatctat gccatcactt gctgcaaaat agtctttgtt
     1981 ttcctctttg ttcgcatttt taactaattt tgtaaaccaa gcatttcctc cagtttccaa
     2041 gttaacaccg ttataaaaaa tcaaatctgc ttttgaagtt ttttgaacat cttcaggcaa
     2101 aggttcatat tcgtggggat ctttcccaat aggtacgata ctgtgtaaat caattttatc
     2161 ttttgctata ttttcggtaa tatctgctaa aatcgaatta gtaactacta cttttaattt
     2221 ttcgttcgaa gccactttat ctttgtcttt actatttatt gaacatgccc cgaatataaa
     2281 aagagcaatc aaaatcccca gacttcccag tactgctttc cattttctca tcataacgat
     2341 ctctccttct ttcttaaaaa tttttgtttt ggtgagatta gaaaactaat tacaaataag
     2401 aatgccgctg ttaaaacaat acttgatcct gctgctatgt taaaactata accaataaac
     2461 aatccaagca atgatgaact tgctccgact gttgcagaaa taaatatcat agttttcaag
     2521 ctatttgcat ataaatatgc tgtagctgct ggtgtaatta acattgcaac aattaatata
     2581 gttcccacac tttgcatagc tgttactgct actaaagtta atagaaccat caatagataa
     2641 tgataaaaac tgacattcat ccctattgct tttgccatta atggatcaaa agaagtgatt
     2701 aataattctt taaaaaatag aactactata accaacacta aaaccgaaac gccaattgta
     2761 atccacttat caatatcttg tacagctaaa atatttccaa ataaaatatg aaataaatcc
     2821 gttgagctat ttgcaactcc tataaggata actcctagtg ctaaaaaaga actgaaagta
     2881 ataccaattg ctgtatctcc ttttattaca ctattgcttt ttataaaagt aataatgata
     2941 gaagctaata gaccaaatac aatcgcacca ataaaaaaat taatacctaa aatataagaa
     3001 attgctactc ctggcaaaac agcatgagaa atagcatcac ccataagaga cattcctctt
     3061 aaaataataa aacaacctac tgttccagaa acaatcccta taactacaga tgtaattaga
     3121 gcattctgta aaaagtgaaa gtcatacaga ccattaataa aattttgaag catattattc
     3181 cccctctcca ataaatatag tatctccata ggcttttttt aaattttctt cattaaatac
     3241 agattctaca cttccgtgag caattagatt tcgattcaaa agaataatat tatcaaaata
     3301 cttctttacc ttgcttaaat catgatgcac aattagcact gtttttccct gatgtttcaa
     3361 tttttttaac gtatccataa ttattcgttc actaactaag tctatcccta caaatggttc
     3421 atctaaaaat atgaaatccg cattttgagc aagacatctt gctaaaagta cacgttgaaa
     3481 ttgtcctcca gataattctc ctatttgatg attagaatat tttcccatat cgaccttttc
     3541 caaagccttt gaaactcttt gccattctgc atttttaatt ctttgaaaaa ctttcattcc
     3601 ggcatatgtt cccatagata cacattcttt tacttttatt ggaaaagtaa aatcaatatc
     3661 agctttttgc tctacatatg caatcttcac cagtttttta ttgatatctt cattattaat
     3721 ggagacctct ccctgatgag ggataatatt taacattgct ttaagcaatg tggattttcc
     3781 agcaccattt ggaccaataa tacctgttaa agcaccttct tgtattttta aagaaatctc
     3841 gttcaatgct aaaaaatcgt tata
//
LOCUS       DAZUKC010000149         3826 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000149 DAZUKC010000000
VERSION     DAZUKC010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.155"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1086
                     /locus_tag="ACG80T_002702"
     CDS             <1..1086
                     /locus_tag="ACG80T_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4842305.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1304..2446)
                     /locus_tag="ACG80T_002703"
     CDS             complement(1304..2446)
                     /locus_tag="ACG80T_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4842306.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2451..2636)
                     /locus_tag="ACG80T_002704"
     CDS             complement(2451..2636)
                     /locus_tag="ACG80T_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFS4842307.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(2990..3235)
                     /locus_tag="ACG80T_002705"
     CDS             complement(2990..3235)
                     /locus_tag="ACG80T_002705"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4842308.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3229..3630)
                     /locus_tag="ACG80T_002706"
     CDS             complement(3229..3630)
                     /locus_tag="ACG80T_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS4842309.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
BASE COUNT         1256 a          697 c          593 g         1280 t
ORIGIN      
        1 gatatcaatg atgatggctc aagaaaaaaa gtggagaaag ttggcggaga cacccgacca
       61 gaggccgaag ctgctttacg aaaagtttta tcagatattg acgaaacagg acaatacttt
      121 ttaggtacgg atactcgagt aaaacaatac cttgattttt ggatggagga atacgttaaa
      181 ctaaatctaa aatacaatac ctatgaaaac taccgattta ccatcaaaaa tcatataaac
      241 ggttatttag gaaagaaaaa acttacggat ctctcccctg ctcttttaca aaatttcatc
      301 aatgctgaat ttaaaaaggg ttactcgaag aaaacaatga ctattactca ctctgtcctt
      361 aagaatgcgc tgaatatggc ggtttatcct tggggattaa tcaagcaaaa tcctatgctg
      421 tatgtaaaaa taccaaaata tgaagcacga ccaacgacta aaaaagatct aaaaatcatt
      481 tctcttgagg actttgatca tatgctagaa attactcctg aaggacatcc tttctatatt
      541 cctttgaata ttggatttta tacgggaatg cgcgttggcg aagtttgtgg tctgacgtgg
      601 gatgatgtcg atttttcaaa tggaacaatt actgtagaga aacaaatggt aaagaatgac
      661 ggcgcatggg tatatggtac gccaaagaca agcagttcca atcgaacgat ttttattgga
      721 caaaccttgc tagcaattct gaaaaaacat aagaaacaac aattagaaaa tcgaatgaag
      781 tatggaaagc tctacattga ttcaaatgca gtatgtacga aggaagacgg tgagctagtt
      841 acgccaagtg tagtgaaatg gaatactagg aggatatcga atgcactctc cctctctttt
      901 aacttccatt ctctcagaca tactcatgct acacttcttc tcgaaaatgg cgcaaaaatg
      961 aaagaaatct ctgaacgatt gggccacagc agaatttcaa ttacgatgga tacttactcg
     1021 catgtaacag ataagatgag aaatgaaacg gtcgatatca tggaaaatct tagaaagaat
     1081 tcttgatttt tgccaccgaa aaaaaccacc ggtggcaaac cggtggcaaa tccatgtaaa
     1141 catggtttat tttttacact gttttcaaac aaacccttgc tatttcttcg gttcgtcctc
     1201 gtccattttc gataccgaca gctagcttat aacccaaggt atatcaacat aaaaaaataa
     1261 aaataggcta tgacagcagc gtgacagcaa aaaagatgga aagttaaata tcaaacctcg
     1321 taatttcatt tgcgagtgct tgatttgttt ctggatataa gtgtccatat ttgtccaaag
     1381 tagttttaat agaggcatga cctagacgct tagatattgc atagatattt ttattatgtt
     1441 caattaaaaa ggcaacatgc gagtgtctta atccatgcat atgaattggt tttactcctg
     1501 caatttcagc tagttttttt attctgctta aaatagttct tggagatggc ggtaatccat
     1561 caaaagaaaa aaccaattct atatttttcc caattgattg ttgagttttt ttccatgttt
     1621 ttaaatcttc aacggtttgt ctgtctaacg aaattatccg aatacttgat gtattttttg
     1681 tttcagcaaa ttcataatct tgtcttgtac ggatgtaaat tgacttattt acgctacatc
     1741 tgccatttat taagtcaaag tctttccatt gtaacgctaa gagctcctca ctccttacac
     1801 ctgtaacaaa aataaaacgc atcaaacgtt tataaaattc ttcataaaaa tccccgtgat
     1861 aaacactatt atatactttc ttaaattcat caactgtcca aaattctact tgaggacgct
     1921 gagtttttat tttacctata gtatctacag gattttcttc aataatttca agtacaattg
     1981 cccttttaaa aactactctc aacttattga atatctgttt aatataattt ggtgataatg
     2041 gttgctcaat gccctttttt acaacatgga ttttcgataa aaactgttga aattcctgta
     2101 tatgaattgg tcgaatcttt acaacctgca tttctccaaa aaaatccaat gcacgttgta
     2161 aaatatgatc tgttttcata tatgtttttt ctacagttcc caacttatac caaggaaaaa
     2221 aatactctcg ataaaaatca tgaaacgtta ttccttgcag ataaattcct tgtgaatcag
     2281 aaaaactttg ttttaactct tcgagtcccc ttctagcttc tccttgagtt ttgaagcctc
     2341 ttctggtcac ttgaattctt tttccaaagc gatcttgtcc taaagttgca gaaaaaaacc
     2401 aagtgcctct ttttctgtct ttataaacat tcgcaatttt cctcatttta ctattcctcc
     2461 caagaaatat ggagaacttc ttctaaaatt gaaataggaa ttctttttat ttttgaatta
     2521 cgataaaaaa agtatccttt ctcaactaaa agttctttac attccttgaa gattttatta
     2581 gcatagtact gagaaaaacc tagattaatc aaatcttttc gagtagctgt tttcattata
     2641 gcaccttcct tacttttttt catttatttc tatcagctgc ttttgtcaaa agcacatagg
     2701 aatttcacct ctccccaagt ttacggcgag ccttacggaa gtatcattat cccaacttta
     2761 aatcatcgct ttttattgac tataaaaatt attttagaat atttatcgct ccttctttac
     2821 agcaagaaat catggcgcaa ctaaaatatg ctccttaaag tgttaacgta ctgatagaat
     2881 ctatttaatt ttcaatgaac aataggtttt ctacctaaaa gaatctacta attccttaac
     2941 tattagaaga ttcttttaaa cagaaaaagt ggaaagggga tttttttaat tagttaattt
     3001 taaattttaa gatcgcttct attaatttaa tttctagctg acttatcata tattcatcta
     3061 cataggtaac gaattgacct acctcgttaa atccatttgt caaacataac ttagccatat
     3121 aagaatgata atgtttgagt atcatcttga tcgaaattgg atctccttct agagcagata
     3181 agataatgga tagtggaact aataggtact gccgcttttt agatttatct accataggtc
     3241 ataccccatt ttcaattgtt caactgcctt ctttcgttga taccagatac cacttgtgct
     3301 catattgaga attttaccga tttcagcatc attaaaacct gcaaaataat aaagtaggat
     3361 aatctttctt cttatctccg ataactgatc aattgctgct gataactttt gatctgaaat
     3421 taatatttcc attccaaata agaaatagac atcttgttca tcaaaatgag tttgggaata
     3481 cctgccttca tttgaatctg ccaatggttc ctcattatcc tttatgttag cgtttcgttt
     3541 tcgaatagac ttagcttcgt ttcgtaatac ttttttacag taggcatcaa attggtgttg
     3601 tatagatatg tcattgtaat gttgttgcat gataatatgc tcctttttcc ccctttccac
     3661 taatacaacg taagcgcccc atagaacagt accttactaa caaatgcacc tcaagatata
     3721 cttgagctat caagaagtct ggaggttttc tatgtataaa ttaaatgaga ttgttcctgt
     3781 ccaattagga aacgaaaaga gtgcaccaga aaaaggtaaa cccgcg
//
LOCUS       DAZUKC010000150         3789 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000150 DAZUKC010000000
VERSION     DAZUKC010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.156"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(147..533)
                     /locus_tag="ACG80T_002707"
     CDS             complement(147..533)
                     /locus_tag="ACG80T_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS4842310.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(801..950)
                     /locus_tag="ACG80T_002708"
     CDS             complement(801..950)
                     /locus_tag="ACG80T_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842311.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            1412..1624
                     /locus_tag="ACG80T_002709"
     CDS             1412..1624
                     /locus_tag="ACG80T_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842312.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(1902..3701)
                     /locus_tag="ACG80T_002710"
     CDS             complement(1902..3701)
                     /locus_tag="ACG80T_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFS4842313.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT         1296 a          675 c          652 g         1166 t
ORIGIN      
        1 cgattgattg ccttgctctt tagtttgttt taggacttta agaacagtga ggctgacttc
       61 gaaggttaat tctgtttttt tgttttctag aatcagtaag caaaaaagca ctcacttcga
      121 gtgctttttt gcgatatatt cttagcttaa gataggccta atttttcttt tacagatgag
      181 ggtaccttat cgctggtaat tacttttttc tctatgatcc gcttttcgct tgctttcaac
      241 tgcaagatcg ttccttcggt gtaagcagaa ccattggcag agtcaccaga agtaatgaca
      301 tcgaaatcta actgtctttt ttcaccgttt tcagcataag catctacgtt gtatttaaaa
      361 cctcttccaa ttacttcccc attatctgct ttgatatcag tttctgcagg cattggagct
      421 ttgactaccg cataataatc tttacctaca taagtagact gatagtagtc ccaacctttc
      481 catcctataa aagcaatcaa tatgataacg atcaacccta aaattttttt catgaaagat
      541 ctcctttttt atttttatta tattaaatat tatacagata ataacggaat attatcatcg
      601 aaacagctaa caaaattgtc ataaaaaacg agatggttga tattagaact tgtgtttttg
      661 tttaggtcta gagataaaga gagacaattt ccaaatatat gtaatgaata caaaaagacg
      721 tttgtcgcgg attaccgtta caaacgtctt ttattgattt tccgagatct catcagtcgc
      781 catttggaat ggttcactcc ttatctagat tcgtttaatg ctcggactgc ttgttcgatt
      841 gtaataccaa acgcaacgtt attttcgttt tgtgcatcaa tcgccataaa acggttagaa
      901 gttgtatcaa aagctacgcg gtattgcata tttgtttttg taaacgtcat aaaattcatc
      961 tcctttgtaa tgtaatttag ttcttgaaag tttcctttaa actttcgaac ttataccaag
     1021 tttagcatcg tacgtttttt gatgcaatta aaaagcattt atcattttcc ttatttattt
     1081 tttcttaaat ggtttagcta ttttctttta atccgataag tagcccaatc atcaaggtta
     1141 caaatactat tgcttgaata taaatcatat gctcacctct ttataaataa cttagcaata
     1201 taaagaaaaa aacgcacgaa caacatagga atataaacaa acgtttttgt cacaacgtct
     1261 aagatcgtgt taaaaaatag ttatctaaaa agcagaaaga tactcaaaat aaaaaataaa
     1321 aaaatgaata aaaacgtttt caaatacaag gtaatttgtt attataaaat aaagaaaaaa
     1381 caaaataaaa ataaagaaaa gagggaatga aatggtaaaa gcagaagtag gaagatctta
     1441 tatatgcaaa ccgatcggag tatctcatga actaatcggc tgtattgaac gtacatatat
     1501 caacacggct gtcgtgtatg ttgaaagtta tgaccaacga gactatcaac ttatcaatga
     1561 aagcaagtat cgcatgctcg taaaattctg tgatattagc gcacctactg aattggtttc
     1621 ttaaacagta tttttgagaa ttcgcaagaa ctcccttata atataaataa agacagaagg
     1681 gagcgagaag atatgccaaa aagaaaaagt tcagggaaaa tgggaattat tcttgcagtg
     1741 atctctgcaa ttcctgctat tttagctatt gttcgtgcga tgaaggaatc aagagaaagt
     1801 taagcacaca ttatgagcgt caaacaaaaa acgcatatac ctgcggtggc aggttatgcg
     1861 ttttttgttg tataaagact tttttcatta acgatttttg attagccgac tcccaaaaca
     1921 aaatttagca gtttatctga ataagtgatt tcttcatctg tttgtttcac actttcattt
     1981 agtagtccca tctcatcggt tatgatacca tccacaccat aaaacatcat gcgactcatg
     2041 gcatctgaat cgttgattgt ccatacatat acttcttttc cgtcttggtg ggcagaatta
     2101 atgaagttac ggttgatcgt actatattcc attgtcagaa aatcagcagg cgtcacaggg
     2161 ggcccaacaa tattgaaagg taagatgtag cccacataaa agtttggttc tgcttctttt
     2221 aaatctgaaa cggtttggta cgtcaaactt tgcaaaatat gatggttctc taagataact
     2281 ttgcggtatt tatggacaaa tcgttcgaca aggtcatccg aatctttttt agaggtcttg
     2341 atctcaataa gtaatttttg gtttagctta tgtgcttttt ccaaataaga atcaaaagaa
     2401 caaacagggg cttgcactcc gttttcagta actgtaagat ttgtcaattc tttcaatgta
     2461 agatcctgtg gtgttccatt tacccctgtt aagtgacgaa gatttgcatc atgcatcact
     2521 acgaaatgac catcttttgt ttcctgaaca tccatctcta cataatcagg atgatagtct
     2581 ctgcttgttt tttcaagtgc ttctaaagta ttttgcgccc cattttgggt actcacacct
     2641 ctgtgagaga cagtcactgg ttttgtctga acagctgaag tcagataatc catattgtaa
     2701 agacagacgc caataccgaa aaaaactgca aaaagagtca aaccagtgtt tttcagcgca
     2761 gagaaacgca agttgggggc ttgtgggata aaccacttag ggatctcagg caagaagcct
     2821 tcgtcatcca tgaaatcaac aatgatataa aaaatcccta cggtagacat cacgatattg
     2881 aataataaaa tcccttgtaa aagcgtcata gcaaacacag cgctgatcaa tgcatagtct
     2941 gggaaaaatt gttcaaccat actttgtgcc aaaataacaa cgatatatcc taaagaagaa
     3001 aggagtaaaa tcgtaccgcc aatcacaata aattggcccg tgatggccaa aagacgcgat
     3061 ttggtcaatg accagctttc ccgaatggct gctcgaaaag aacgatctcg tagaatcatt
     3121 tccggtaaag caaaaatcag tcgaatccca atatagccaa ggaaaatata aacgagtaaa
     3181 aaggaactga cgatgatcca acgattagca aaaataaaat ccataatgaa agcggggatt
     3241 tttatttttg acagtagatc agaattaaaa cttaatccgc tgatcggtag aatcaataaa
     3301 aaataagcaa ggaaaaacaa aaaagtaatg ggtctcactt tcttcagctg aagtatcgtc
     3361 aagtgtaaga gttgcttcaa agagattggt tctttctttt taataaaaaa aacactcatc
     3421 aaaagaaaag taaattcgaa ataaactaac agtaaaatcg tcatcaaaac tacgattaac
     3481 gagagtagaa ccccaggatg ttgagaaaaa attgaaggaa tattgtcata agacaaatag
     3541 tcaattgccc ctcggtgtaa aataaaacgg gtcgtactgc tcaataaggg taaacaaata
     3601 aataaaataa agccatgcat cagcaagaca tcacggaaat aagcttgtgt tccttttaaa
     3661 aagtcccaag cattacctaa gctacgtttc aaataattca tcctatcacc tgctcttcac
     3721 attgtagcgc aaatctaatg aaaaaggaaa gggtgcagtg tgttttgcag accctttccc
     3781 agatttaac
//
LOCUS       DAZUKC010000151         3754 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000151 DAZUKC010000000
VERSION     DAZUKC010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.157"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..1092)
                     /gene="uxuA"
                     /locus_tag="ACG80T_002711"
     CDS             complement(13..1092)
                     /gene="uxuA"
                     /locus_tag="ACG80T_002711"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS4842314.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(1095..2726)
                     /locus_tag="ACG80T_002712"
     CDS             complement(1095..2726)
                     /locus_tag="ACG80T_002712"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFS4842315.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            3030..3701
                     /locus_tag="ACG80T_002713"
     CDS             3030..3701
                     /locus_tag="ACG80T_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4842316.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
BASE COUNT         1222 a          730 c          524 g         1278 t
ORIGIN      
        1 tcccttatat aattatttac tcgccccctt ttcaagcgct tcccaaatac catttaaata
       61 aactgcacct agagctcggt catataatcc ataaccaggt ctaccggttt ctccccaaat
      121 catccgacca tgatctggac gaatatagcc attaaatcca ttatcgtaca ttgtttttaa
      181 tatcttatac atatctagag aaccatattc tgatttatgc ggcgcttcgt aaaaatcgag
      241 tccattgtca cttaaatatt tgatattacg aacatgtgca aaaggaatcc gatcccgttg
      301 acaaaattca gatagaattt catagacatc attatctggt cgttctgcaa tacttccagt
      361 acataatgta ataccattag aaggagaatc cactacatta cacaaccaat ctaaatcgtc
      421 acgattttta acaatacgag gtaatccgaa aatggaatat ggtggatcat ctgggtgaac
      481 tgccatttta atcccaactt cttcacatgt cgggataatt gctttgagaa aatatgcaaa
      541 gttctctcgt aatttctctt catcaacttc tttataggct tctaataatt gcttaatttg
      601 tgataaccga tgaggttccc aacccggcaa ttcaaaatta ttagtagaat tttctacttc
      661 tctaataatt tcctccggat cagtagggat attttttgaa atataggcta acgtagaaga
      721 cccatcatct aaacgataat ccaaatccga ttttacccaa tcaaatactg gcataaaatt
      781 ataacagata acctcaatcc caaattctgc caaattgcga atcgtctgtt tataattctc
      841 aatgtattga tctcgtgttg gtaaaccaat tttgatatca tcatgaacat taacactctc
      901 aataactttc aaggtaagtc cattggcttc gacttcatct tttaaagatt taattcgttc
      961 tctcggccaa acctctccgg ctggtatatc cattaatgtt ccaacaattc ctttcattgc
     1021 tgggatctga cgaatttctt gtagtgagat tttttcttca tttgccccat accatctaaa
     1081 agtcatttcc atagttactt tcctccaact tctgttatat atttattgat tgttttacga
     1141 actgcttgag gacctactag catttcgcaa aagatttttt cagttttctt tcctacccca
     1201 acttgatata aatctaaacc aaagatttct ttattttcta aaatttcctg taccgcacaa
     1261 tgaatctttg aaggatcttg ttcacctaat ttcaatactg ataatttatc ctgcaactct
     1321 accaataacg gatcaggact tggcacaaaa ttttcccctt gatcatcgac tgccagtaaa
     1381 taacgtaacc aagcagcaat agcgatagga ataaatgtta gattttctgc atttccgtct
     1441 ttttcgacat actttttaat tgtttcacca tagcgaatcg ctactttttg agaagtatct
     1501 gttgcaattc gttgtggtgt atctggaata tagggattag gcaaacgttt attaattact
     1561 tcatcaataa attgttttgg atcaataatg cctggatttt caactactgg taaaccttct
     1621 aaataaccta ttcctttgat taaaccaacc aaatcatcat cctgcatctc tgctgaaatt
     1681 gattgatatc ctagcagaca acctgttact gccaaagcag tatgaagtgg attcaagctt
     1741 gtcgtaacct tcatgatgtc tgctttatca accgtctctc tatctgtcat aatcacacca
     1801 gccttttcca atttaggccg gccatttgga aaactatctt caataactaa ataatgaact
     1861 acctcagtat tagtgaaagc agcaatattg gtatgttttt ttgtgtgaat gatatctaaa
     1921 tcctcgagcc ccatcatcaa tagatcttcc tttacattct ctgaaggatt tggcgtaatt
     1981 cgatcaatca tactccaagg aaaagtcact ttttctttat cctttaaata agcaataaat
     2041 ttttcagaaa catatccttc tttttgccaa gcttctgcaa tcgtaaaaat actatcttga
     2101 aaacgttgac cattctgtga gaaattatca gtagaaacca tcgcaatcgg caactctcca
     2161 gcattaaatc tgctatataa caaggatgtt acaatactca tcgtatgttg aggattgcta
     2221 ggtccagatt tgatatcagc tttgactacc gaaaagaatt gccctttaga atcttttaaa
     2281 ctgtaacctt tttcagtaat agtaaacgtt gtaaactgta atttaggatc ttcaaaatat
     2341 ttaaccactc tttgatagtc cgctatattc ttcggattgc aatataaact atcagccgtt
     2401 actgccaaaa gtcttttttt gagttcacca tctccactca taacaatttc caaaatgtca
     2461 ttgttatagt catgataagc tttttccact acttcttcat caaatgtttc acacacaact
     2521 actcctgttt ctaattcatt ttgatctgct aagcattgag caatttctgc atgaaatcct
     2581 cgataaagat ttcctccacc aaaatgaagc catactggat gttgctggcc ttcttgctta
     2641 acttctttga catcaaaaca cggcagtaga atctgcattt cattgaattc ttcgatagaa
     2701 ttttgccaat ttttaatcga aaccataatt aacctcctca aactgatatg ttagttttga
     2761 tacattttat attttaaaac ccattgtaac cgttgtcaat ataaaaaggg aataaacttg
     2821 cttttttcta ccatatcaga atgattgtat tttttattca gaaaaataaa atactccttc
     2881 tttttggact gattttatcg aaacttattt tttctttttt tcatgtattc ttttattttt
     2941 ataactagtg tattacttca aaatttgata ttatatggca tgaacagaat taaaatatca
     3001 gttaaaataa aagttaagga gcattgtgta tgacaattaa aactagtctc caaaatcaag
     3061 cttaccaatc tattcgccga caaattatct atgctgactt agaacctgga aaaaaagtat
     3121 cagaaaaaaa attagaagaa acactttcta ttggtcgtac accaatacgt gaagcactga
     3181 ttcaactccg acaacaaggt cttgttgata caattcctca aagcggaaat tatatctcat
     3241 taattgattt agattcggcc aaaaatgctc gatttgtcag agaacatttg gaacgacaaa
     3301 ttatgttaga gtgttgtgcc aaaatgaata accaaaaaaa acggatttta gaaactataa
     3361 tttcagaaca agaaaaagct gttagtcaaa aaaatgctcg tagttttttt cataatgata
     3421 atttattcca taaagcttgt tttgaaattg cagagcgaga agaagtatgg caatggttag
     3481 aaaataacaa tacacactta gaacgatttc gatggctacg agtaaaaacg gaaggattaa
     3541 agtgggaaaa aattatggaa caacattatc aactatttga ggcattaaca aaacaagatc
     3601 cagaagaagc taactttcta acatcgttgc atcttcattt aatgcttaat gagcaaaata
     3661 ttgttttagc tactcatcca aaatatttca aaaatatata acttttaact ctttttaaat
     3721 taaatggttg ttctgatcag aaaaatatcg cttg
//
LOCUS       DAZUKC010000152         3668 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000152 DAZUKC010000000
VERSION     DAZUKC010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.158"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..254)
                     /locus_tag="ACG80T_002714"
     CDS             complement(<1..254)
                     /locus_tag="ACG80T_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008177227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4842317.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDT"
     gene            complement(244..1443)
                     /locus_tag="ACG80T_002715"
     CDS             complement(244..1443)
                     /locus_tag="ACG80T_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS4842318.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(1573..2949)
                     /locus_tag="ACG80T_002716"
     CDS             complement(1573..2949)
                     /locus_tag="ACG80T_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4842319.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2949..3605)
                     /locus_tag="ACG80T_002717"
     CDS             complement(2949..3605)
                     /locus_tag="ACG80T_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4842320.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
BASE COUNT         1125 a          687 c          512 g         1344 t
ORIGIN      
        1 gtatcgactt ccttaatggt ttgtcgcatg gaacggatac cgaataaata ctgaataaac
       61 accaatttga tcagaatcac tggatcgata cttgggcgac ctaaatcgga gtaagtggct
      121 tcgacgattg gataaataaa ttcaaaatca agcgcggcat caatctttcg aacgagatga
      181 tcttgaggaa ctagatcatc aatcgtaagc atcgcaaatt gatttctacc attcgtattg
      241 tttttagaca tcatagctat cactccttgc attcgttttc ataaatctat tataaaagaa
      301 aagactgtag acatcatcca taaaatggac tttgtctaca gtctgcgcat tctcattaga
      361 gaacactccc ttttttgtga cttttctgaa gctagagtga atactttcaa ctgcattagt
      421 agtgtacatg atttttctaa tggcgctgcc ataatcaaag agttgttcaa catgagaaaa
      481 gtttcgtttg catacatcta ctgcaccaga ataatgagac caccgatttt gaaaactgtc
      541 gaaggcagcg tgtgcagcgt ttaaagaaga agcactgtag aattttttca tgtctcgaca
      601 gacttcctta taatccttac ttgggaggta tcgtaacgca tttcgaacaa gatgaacaat
      661 acagcgctgc acaatgacag aaggaaaaat tgcttttgcg ccttctttga gaccggaaac
      721 accgtccatc gaaatgaaaa aaacatcttc tacaccacgt actttcagtt catcaaatac
      781 ttgcatccag cgatttttag attctgtttg attgagccat aggcctaaaa tctccttgtt
      841 tcctttaaga tcatagccaa gaatggtgta cacagcatat tctttagctt catagttttc
      901 gcgtaaagtg acgtacatac aatcaacgaa tagaaatgca taacacttgg ctaatggccg
      961 agcttgccat tcttccaatt caggaaggac ggcatccgtg atatcagaaa tcatttcgtg
     1021 ggaaatatca aagccgtaaa tatcttcaac ggttgcggcg atatcgcatt gactcattcc
     1081 tcgtgcatac atggaaagaa ccttaccttc gatgtcggag acatctcgtt ttctattagg
     1141 aatcaactca ggttcaaaag aagcctcccg gtctctggga acatcaatgt ttactttacc
     1201 aaagctcgtt ttaagcgtct tattgccata accgtttcgt cgattgtcat gtttctttgg
     1261 ctctttagaa tgggcgtcat accctaaatg gttattcaat tctccttgaa gcattttttc
     1321 aaataaggga ccaaacacat cttttaaaac atcttgcata tcctctacag attcgggttg
     1381 ataagtattc aaaatggatt cagctaactt ctcagcatca ggatttcttt tctttctagc
     1441 catcgtgtaa tcacctcttg atcttatttt agaaaaataa aaaccgtgaa gcaaccaccc
     1501 gcctaggatt gatggttact tacacggttt atattacact ctcatttttg gtgctaaaaa
     1561 gcaccgaaaa ttttaattat tgtcaattaa taattctttt ggattccttc ttaatatatt
     1621 gaatgaagaa ataatcaatg atacaagaag tactaaactc ataaatataa ctacataaat
     1681 catgaattta ggtaatacat tgatatctaa acttgataat gtcttgttaa atccttcagc
     1741 ttctgcccca ccacctaatt ggctagatgc agattgttta gctatttgtt tagctatatc
     1801 accagtaacc ttattcaata tattattccc aagcttgtca gctgtatatt gagctaagaa
     1861 ataggaacca acgaatgcgg ggattgatat aaataccatc tcaattagga attgaccaaa
     1921 aatttctaat tttgatatac ctaatgatag taatactgct atttctttct ttctagcatt
     1981 catccataag aataataata atgaaactac aactcctgca aatattaatg agccaaaaaa
     2041 taatttattt gaaattgagt aaatacctga tatagattgt tgtaatgcag ggtaatttga
     2101 tgaactttta atcaaattat attctctcca atttatatct aatttcccaa gatcttttat
     2161 tacactatca agattcttat caccttttac aaagaatgtt gcatcttggt atacagctgt
     2221 atcttcggta ttaccataaa ctttagcagc tgtatgaata tcagtgatta atgtattttc
     2281 gtatagttct tgtgctgcac ttactccacc gctattatgc ccatcgaata gacctttaat
     2341 ttctacttct actgtttcat ctgcaccttt ctcattatca gcatcaaata aattagattt
     2401 tatttttatc ttatctccaa ctttaaggtt atttttttca gctaaatctt tatgcattaa
     2461 gattttattt ttatcttcat tttctaaatg ctttccttct actaatttat atgcttctga
     2521 tacaaatttt gtttcttttg ttgagtcgtt aaccccagtt aacataactg ttcttttaaa
     2581 attctttgct ctttcaggtg attgattcgc aagagtctct tgagtttcaa taatatcata
     2641 atcaactaaa tctgcaacac tgtttattct ttttacatag gagtctatac tatctgtttg
     2701 agatatcttt ttaatatctt caccttttac atttccccca cctctaggtg ttcctggatt
     2761 tacttgtcta tttatctcca tagaaaaact atttgttata ttagcaaatg tttcttttga
     2821 agctctgtcc gtagcatctt taatagataa actaatgaga cttaaagttg acatagataa
     2881 aataactaat atgataatta atgattttaa actttttcta gttacataag caaatgcatt
     2941 ttttatcatt attcaacctc caaattcatt ttatttactt ttttcaactt tttaccactt
     3001 agttctaaaa tgatatccgc agaatctgct acttccttac tatgtgttac aactattaca
     3061 catttatttc tatctttagc taatgtcttt aatatattaa ttatttctcc agcagtaaca
     3121 ctgtctagat taccagtagg ctcatcagct agtattattg gagcatctga taccaatgct
     3181 ctagcaatag ctactctttg ttgctgtcca ccagataatt tcataacatt tctttttatt
     3241 tgttttttat ctaaacctag ttcaaacaag atactttcat ctgctgattt atttactaat
     3301 ctaatatttt caattggtga taaataatct attaaattat agttttgaaa tactaaagat
     3361 atattatttt ttctatgatt actatatcct ttattttgta tatcttcatt cttaaacaat
     3421 atttttcctg tttgaggttt atcaagtcca gcaagtaagg aaagaagtgt agattttcct
     3481 gttcctgact ttcctactat cgcataaaac ttcccaagtt caaatttttg atttactcct
     3541 gacaaaactt tttctttaga atttgcataa ctatatgcta cattctttat ttctaatata
     3601 tccattattt tctcctaact tatttttgat aagatttctt taggcttctt gattaacatt
     3661 aatgaaga
//
LOCUS       DAZUKC010000153         3620 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000153 DAZUKC010000000
VERSION     DAZUKC010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.159"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..1465
                     /locus_tag="ACG80T_002718"
     CDS             134..1465
                     /locus_tag="ACG80T_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFS4842321.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            1431..1781
                     /locus_tag="ACG80T_002719"
     CDS             1431..1781
                     /locus_tag="ACG80T_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842322.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1873..2292)
                     /locus_tag="ACG80T_002720"
     CDS             complement(1873..2292)
                     /locus_tag="ACG80T_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFS4842323.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(2293..2637)
                     /locus_tag="ACG80T_002721"
     CDS             complement(2293..2637)
                     /locus_tag="ACG80T_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFS4842324.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            2987..3583
                     /locus_tag="ACG80T_002722"
     CDS             2987..3583
                     /locus_tag="ACG80T_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFS4842325.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT          956 a          653 c          654 g         1357 t
ORIGIN      
        1 tttttttgca aaattaaaaa aatttggtta aattcaatgg gttcccttac atttttagtt
       61 ttgacctaaa tgatagttgt ccaaatcttt catttcaagt tatagttaaa gaaaatgaaa
      121 aagaaggtgt gtaatggaaa acgaaactga cgttatttat attcatcctc aaaaacgaat
      181 cgtttcacag aaaagaaagt atttttatct tggttttaca ggtgtttttt ttctttttat
      241 tggcttgctt tctaatactc ctactgataa ttggagtgga ttgctaacta ttcttacctc
      301 gccaagcaat cttttgacag attattttgc tttaggtgtt ttcggaagcg cattcataaa
      361 tgtagggatt ttgaccttac tttctgtgct tttagcttat cggcacaagg tgattttaaa
      421 cggtccctta tttgcttcga ttctaacagt taccggtttc tctttttttg gcaaaaacct
      481 ttataattct atctcaatca ttttaggtgt gtatctatac gctgtatttg tcaataaacc
      541 tttttctcag tacatcatga tcggactgtt tggtagcgta ttatcaccgg tcgttagtta
      601 tatcactttt ggtatgcgtt ttcctttact gataggtatt ttattgggga atttagctgg
      661 catagcaatt ggtatacttc ttccgccctt agctgctcaa actttagttt tccatcgtgg
      721 attcacactt tataatattg gattcacctc tgggttgatt gcgatgactt ttactgctgt
      781 tttgaggtta ttcagctatt caatcgtcga aaatacattg gttttcaatg aatatcactt
      841 tcctctcatt tggattattt ttggcttttt ttcgctgaca gtcggaattg gtttctatta
      901 taattccttt cggctttcgg ggattcgaga aattttcgat tcatctggaa aattgacaac
      961 tgatttcatt gctaattctg gtattggtgc tactttgatc aacatgggct tagttggtct
     1021 gatgctgtct tcttatgttt tgctggttgg cggtcagtta aatggacctg tcatcggtgc
     1081 tattttatct gcggtgggtt tcagtgcttt tggttgtcat ttaaaaaaca gttttccgat
     1141 actagtcgga atatttattg cttctttgtt tggaactttt catgagatca ccagtactgg
     1201 catgcttgtt gcagctgtgt ttgggacagg tcttgcgccc ataagcggtt tttacggcag
     1261 tttttacggt gtaatagctg gtgtgctaca tattgctctt gtccataacg tcagcacttt
     1321 gcacggagga ctaaatctat ataacagtgg tttttcaact ggtttcgttg caggtatcct
     1381 tgtaccgata ctagataact ttaccgctgt cagaaaggaa aaaaagacac ttggaaaaag
     1441 aattatcaaa aagaatcatc gatgagcttc ttacttattt atattcccat gaaataactg
     1501 aaatacgtat tggagtagaa tttgctgagg aagggctttt catagagata cagggtacgg
     1561 cagaaactca gccagaagat ttagatgtac ttgctgattt gctgaacaat cctcgggacc
     1621 ctagtatgga aatctactat caagaactat taggtaatcc ccgacatgat cgggaagatt
     1681 atcatctgtt aggtagtttg attgatgacg tggaaatcat gtatgcccag ccgattatca
     1741 atattaagat ctttcgacgt gcttctcatt cctattatta accctatttt ctatttataa
     1801 aagactgaat caaaaaagca gctcctgaaa gttcgattaa aatctacttt caggagctgt
     1861 tcaagcttga cattattgat tttccaatcc ttcatacact tcatctgtta caggttctaa
     1921 aaactctgaa ttaccagcag tgatcgccaa atgttcaaac caactgtctt ttgtcgcacc
     1981 atgccaatgt ttaatccctt tgtgcgttac gacgacatct ccagccatta atcgtcttgc
     2041 tggctttcct tcttcctgat accagccttc tcctccggtc acaagtaaga tttgatagcc
     2101 gtctgtatgg acatgccagt gattgcgaca acctggttca aaagttacat tacctacacc
     2161 aacagaaact tcgggatcat tcactaacat attcagatag ctttgcccgg taaatacttg
     2221 agcgtaagct tcgttttttt cacctgctgg aaaaatcacg ccattgtgta tttcttcgtg
     2281 ttttgtcatc atttacgcct ctttcttatc ttcgctattt tcttctaaat agatttcttt
     2341 tgctaaacta aaagctgacc aagcttttgg ccattctaca taaaaagcta aatgggtaat
     2401 gatttctgct atttcttcac tagtaatgcc attttgcttg ccaatcttca gatgagctgg
     2461 caattgttca aaatttcctc ctgaaattaa tgcagatatg gtgatgatac tacgctgatg
     2521 agcagataat tcttctctgg accatacttg tccaaataaa acatcatcat ttaattctgc
     2581 aaattttggt gcaaaatctc ctaattgatc tcgtcctgcg gtttgttttt ctgccatttc
     2641 tcttattctc ccttcacgac tgttaggtga ttcaactatg gttctaaggc aactttattt
     2701 tctattgttt cttcgcattt cattttaaat gtagtctttc agcctgtcca atgcttagtt
     2761 tattcagtcg atatgctttt taagcatagt tgctaacgct ttttttattt taaaaaaaac
     2821 aagttattaa ataagtcttt agttggatgt ttttttgaga tcaatctttt atacttatga
     2881 tgaacttaca aaattgtttg ttttctgtca tatcaaaaaa tggcgcagtt agttgctttt
     2941 ctagtaaagt atagttaaca aagaacctct aaggagttga tgaataatga atattgctgc
     3001 ctctcgaaaa ctgataaatt tcatatccat tatcggatta ggcctatcaa tcgcgctgac
     3061 gatttatttt attaatttag gtgttttcaa agatttgaat gcgttaagag gattagtagg
     3121 tgattccatc attttaggac cgattatttt tgtcttcatc caaatcctgc aagtagttat
     3181 tccaatcatt cctggaggga tcagtactgc agctggtgtc ctgatatttg gcccttatgc
     3241 tggatttatc tataactata ttggaatctg cattggttcg attatcattt ttctgctagg
     3301 gcgtcgttat ggaaagccat tcatactttc catgattagc gataagacat ataacaaata
     3361 tattggctgg ctggataatc aaaaccgttt tgagaagctg tttgctctag ctatttttct
     3421 tccagttgct cctgacgatg cgttatgttt gatggctgga ttgacaaaca tgtcagtgaa
     3481 acgatataca ctaatcattt tgattgctaa accactgtcg attttcttat atagtatggc
     3541 cttgatttat ggaggtcaat atctaagtgg tcttcttgga taatatttat tttgtaaaat
     3601 tagtcattga ttgacaatgt
//
LOCUS       DAZUKC010000154         3467 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000154 DAZUKC010000000
VERSION     DAZUKC010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.160"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2232)
                     /locus_tag="ACG80T_002723"
     CDS             complement(<1..2232)
                     /locus_tag="ACG80T_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS4842326.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTSNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="ACG80T_002724"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="ACG80T_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          974 a          766 c          549 g         1178 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtattgcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagttttact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacatt tttataaaac aacgaaaaat aaatcactac cagtcaa
//
LOCUS       DAZUKC010000155         3284 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000155 DAZUKC010000000
VERSION     DAZUKC010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.161"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..588
                     /locus_tag="ACG80T_002725"
     CDS             4..588
                     /locus_tag="ACG80T_002725"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4842327.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(664..1839)
                     /locus_tag="ACG80T_002726"
     CDS             complement(664..1839)
                     /locus_tag="ACG80T_002726"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFS4842328.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            1921..2301
                     /locus_tag="ACG80T_002727"
     CDS             1921..2301
                     /locus_tag="ACG80T_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFS4842329.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            2375..2737
                     /locus_tag="ACG80T_002728"
     CDS             2375..2737
                     /locus_tag="ACG80T_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842330.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
BASE COUNT         1116 a          539 c          621 g         1008 t
ORIGIN      
        1 aatatggcat tcccacaact agatttaaaa agtgaaaaag gaccaaaagc tgttatcaaa
       61 acaaaccgtg gtgacattac tgttcaatta ttccctgaac tagcaccgaa gacggttcaa
      121 aatttcattg aactttccaa aaaaggttat tatgatggcg tgatttttca ccgcgttatt
      181 cctgatttca tgatccaagg cggcgatcca actggaacag gaatgggtgg cgaaagtatt
      241 tatggcgaaa gttttgaaga tgaattcagt cgtgaactgt tcaaccttcg cggagcttta
      301 tctatggcaa attcaggtcc aaacacaaac ggaagccaat tctttattgt aaataacaca
      361 aatgtgccag ctaatatgct tggacaacta gaaggtgctg gattcccatc agaaatcatt
      421 gaagcgtata aaggtggcgg aactccttgg ctagatttcc gacatacagt ctttggacat
      481 gtactagaag ggatggacac agtagatgaa atcgcaaatg tacaacgtgg tccacaagac
      541 cgacctgtac atgatgtagt gattgaaaca attgaaattt cagaatagtt tggtatggta
      601 cggagtaatt atgaaaaagg acgaaaccta gtaaggtctc gtccttttat tttgaataaa
      661 tatttaatcg attgtctcta aagcatactt gattcgatta agtccttcca gtaaagtttc
      721 ttctggacaa gcaatattca agcgcatatg cccatgaccg gaaggaccaa acgtgatgcc
      781 aggatttaat acgacttttc ctttccgaac aagagtgttc tccaattctt ggtcgtctga
      841 aatataggcg gtaaaatcta accacattaa gtaagttcct tcagggttca ttattctcac
      901 tttcggaaga tactgttcaa aaaactgatt aactgtttga acattttttt ctagataagg
      961 aatcagttgg cttaaccatt ctccacccgt ctcataagcc gcttgggttc caatcagtcc
     1021 aaaagtgttg atttcgtgct gttggttcat cactaaatgt ttttcaaaat cagctttcat
     1081 ctctggattt ttgataaata ccattgaatt tttgattgca gctagattga aagtcttggt
     1141 tgcggaagta aaagcaacta gtagatcatc taaattagga tcaatcgtta gcatggaagt
     1201 aaatgtatga tcgaataaag ttaaatcttg atggatctcg tcactaaaca gaatcacttg
     1261 atgttttaaa cacaattcgc ttaactgttt caattcttct ttgctccaaa cacgccctcc
     1321 aggattgtga ggattgcata aaatcattgc ttttacattg ttttcttcga tttttttctc
     1381 catgtcagac aagtccatga tgaaatgatt atttttttct agcaaagagc ttcgaacgat
     1441 gtgacgttga tttgtttcta taatagagga aaatggagga taaactgggt catgaatcaa
     1501 aatagaatcg cctggttttg taaaagtttg tactgctgca gctaaactgg ttaacacacc
     1561 acttgtaaaa acgatatttt ctttcgtcaa ctccacagca tggtgttggc gttcccaatg
     1621 aatgatagcc tgatatagtt tatcagacag agtggcataa ccaaagattc catgtttgat
     1681 gtattcacta aacgctttag ctaccccacc gggagacaag aagtccatat ctgctaccca
     1741 caaaggaagt aagtcatcca tttgatatgt ctcagcaatt gcgtcccatt ttacactttc
     1801 tgtatctttt cttgaaatct gtttatcaaa ttcgatcatc ttaagacctc cagtcatttt
     1861 cgtcgcttct ttttttgagc tatttacagt ataatgataa atgcgaagag agggaagtag
     1921 atgacttata aaattggaca aataattgaa ggaaaagtga cagggataca gccttatggc
     1981 gcattcgttt ctcttgataa cgagacgcaa ggacttattc atgtttcaga agtacaatcc
     2041 ggttatacga aaaatatcca ttcattgtta aaagtcggac agccagtaac agttcagatc
     2101 atcgacattg atgaatattc caaaaaaatc agtttatctt taagaacgtt agaaaaaaca
     2161 aatccagctg ttccatatcg cagaaaaagg tatttcacga ataaaaatcg taagatcgga
     2221 tttaccacta ttgcggagca gttacctatt tggatagaag aagcattatc tgaactagaa
     2281 cggaaagaaa ataagaaata aaaagtgtat tttttctgaa aatgtgctag aatttttatg
     2341 taagccatag aacaagttga tcgaggtgga aattttggca agcaaaagag ttgctggtac
     2401 aattatgttg catttggagg atggtactaa aaaatttttg gtccattcaa ttgatgacaa
     2461 gttggaattt gcgttagcgg aactttctga aggaaagact ggtttagcga atatacttaa
     2521 tttcttaaaa gaaatagttc atatcgatgt tagcaagatt agtttgatgg aattgacaaa
     2581 tacacatatt aaagaggaaa atgttccgtt gtttgtcttt gaaactgaac aaaagaatca
     2641 aagagaagaa ttaggcgaag gatatatttg ggaagaacca ggtcaaatgc gctctgttct
     2701 tggaacttac gaagtagagg gagtgccttt cttctaaaat acagattaag cgaacaaaga
     2761 agtttgaatt tttttcaaac ttcttttttt attgtttggt ccaaaaaata caatgaaatg
     2821 ttctgctaaa gatagttttt tagtgcattt tattttcaaa atatgttgaa aacggttgta
     2881 taacgaataa taataattga tcgaaaaatc atttcacaaa gtaatgctta atttatagtt
     2941 gaccaaggaa gaaatacatg ataaatttaa cagcgttgat tctgaagtgg atgttatatc
     3001 acatttctaa aaaatttaaa aaatagttat tgacatgtta ctaacgacat gataagataa
     3061 ctgagtcgtt aaaaaagaac cacttgcgaa gcttgattct atcgaaaaaa acttcaaaaa
     3121 agttcttgac aaataacaaa cgataagtta tgataaatga gttgctgaaa gatttcgaaa
     3181 aacgaaatcg aaaaaaactt ttaaaaaaga gttgacaatc gcttgtctct ctgatatgat
     3241 ataaaagttg ctacggcagc caaaaacgaa tcgggaaaca ctcc
//
LOCUS       DAZUKC010000156         3168 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000156 DAZUKC010000000
VERSION     DAZUKC010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.162"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(63..608)
                     /locus_tag="ACG80T_002729"
     CDS             complement(63..608)
                     /locus_tag="ACG80T_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFS4842331.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(631..1116)
                     /locus_tag="ACG80T_002730"
     CDS             complement(631..1116)
                     /locus_tag="ACG80T_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS4842332.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(1106..1918)
                     /locus_tag="ACG80T_002731"
     CDS             complement(1106..1918)
                     /locus_tag="ACG80T_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFS4842333.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(2057..>3168)
                     /locus_tag="ACG80T_002732"
     CDS             complement(2057..>3168)
                     /locus_tag="ACG80T_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFS4842334.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT          977 a          680 c          566 g          945 t
ORIGIN      
        1 ttaaagttca ttagttggac tttgcgagga agtttttcag attgctctgt caattactca
       61 tttcaataat tcgctctagg accaccgata tatttaggac gagagcgaag ggctgtcacg
      121 tgagcttgtc caagggtttt caaacgaggt ctgacaggta cttggacatg tttgatatgc
      181 ataccgattg atgtgtcacc gatatcgata ccggcttgtc caactacgtg ttccacttcg
      241 acaggatcat tgaactggtc aaaagcagca acagaacaag cacctccagc atgtagtgca
      301 ggaacaacag agacaatttc gaaatttttt gcttctgcca ctgttcgttc tactactaat
      361 gctcggttca aatgttcaca accttggaca gctagcgcaa tttctttagg atccaacaac
      421 tcttttaacg tacggatgat ccattgacca acttccgcac tggaattttt tccaatatgt
      481 cctccaacga cttcgcttgt gctgcatcct aaaacaaaaa gatcaccttt tttcaagtgt
      541 gcctcagcca atatgtcatt aactatttca gtcaattggt cttttaattc tttttctgac
      601 aacttcattt tctaaaacct tctttttcaa ttaatattgc ttccgtgctt gaatacgttg
      661 aagacttgca gttataggaa aggcaagtcc cattccaatc agattttgga agatgtttcc
      721 tggtatagaa gccactccag ccggccaatc atataaaatc caaccggcga taccatagcc
      781 gatgatcata actaagcttc ctaataaaag ccctaatccc cacatacgag tagaagattt
      841 gctgaaagaa ccagcaacat atccttgtaa cccgtggatc actaatgaaa aaactgccca
      901 ctgcggatat cctgaaatca aatcgatcaa tagaccactg gaagcaccaa caactaatcc
      961 acccgttgga ccataaagaa aagcagcggt atagatcccg gcctcgcata atgtcacaaa
     1021 tccattcgta gccggtacag ggatcaaaat tgttaatgaa agggcaactg tcaacgcaat
     1081 cagcatagca tagatcgtta attgcttagt tttcatttct cattcctcct ttttgaatac
     1141 cgccagacca gacacttccg gtatgatcgt tcatcagcac gccattagaa atacaatggg
     1201 caacaaattt tttactttct gctactgctt tggtaaaatc tgcccctaac acaagattcg
     1261 ctgttattgc agaagaaaag gcacaaccgg ccccgtttac tgttgtagtg tcgatttttt
     1321 tatgagaaaa tacctcataa tgatcaattg aatagagaag atccacagct tcatctccag
     1381 ccatccttgt gccgcctttg atcacaatgt tttttgttcc tgaagcagtg agttctttag
     1441 cgagatggat catttctgtt aacgaagagg gaagagcttg accagaaagt aaagcagctt
     1501 ctgccaaatt aggcgtaatc atcgtaacaa gtggaaaaag ttcttcgatg agctgcgaaa
     1561 tatacaattg attgatcaca gcttcagttt ctttgaatgc caaaacaggg tctaaaatga
     1621 ttgggatatt ttgttttgtt tttatgaagt ctcgaacaac catgattgct tcacttgaat
     1681 tcaataaccc aattttgata ccatctaatg aagtcatttt atcaatcgaa actaactgtc
     1741 gtttaagcaa ttccaaagat atagattcca gcataaatcg ctcattaata tctagtgccc
     1801 ctatagaagt caaggcgctt agtccgaata gaccaaaatt ctcaaaagtt tttaaatctg
     1861 tttggattcc tccaccagca aatggatcag aaccgccaat tgtcaagatt cttttcataa
     1921 cattttcctc catgaataag agtataacga aattttcaaa aaagaaaaac agccaattgg
     1981 catattttta tctcatccaa tttaaaaaaa caaaaagaaa agaaagacca aaatggatct
     2041 ttctttcctt aaatagttag gacttttgtg attttaattc tttctcgatt tgaagccgtt
     2101 gcactttcat tgtagctgtc cgcgggattg catcaaaatc acggatgata gggacgttca
     2161 agcgaggaag ttcatggact tgctcccacc atcgattcca atccatttct ttatcaggag
     2221 ctaaagcaat aatcggttgt ggagaatgat ttttatccct tacaatcact acttctgcca
     2281 aaaaatctaa cgaatctaat aaaaagtctt caatagctaa tgtactattg atcgtttcga
     2341 tcaaatccac ttgccgatct ttcaaaaata ggtggccatg cttatccata aaaccgtaat
     2401 ctccgctatc ccaccaatcg ccataaacat tttcctggaa ccgtgcatct tccttgtaat
     2461 aagtcaaagc ccgtccttta gacagtaatt gtatatgacc atctgtgttt tcaggtagga
     2521 cattcccaac tgaatctgta atccgagcgc ttgtcatgtc ttccagtcca acgcccatat
     2581 ctcttgcatc acttgttttc aaactctcta gtgtatgagc cttaagaatc attggtccgc
     2641 attcactttg gccatatact tgaagaaata ccgcatcctg tgccatcgac gttcgcaaaa
     2701 atgtcttcat tgtctggttg tttatagcat caaaagtgga gtgatagtat cggatacccg
     2761 aaaaagcttc tggatgctcc ttagcagtaa aacgccactg aacaaagtta ttcggatggg
     2821 tctctagtgc aattggccgg tgagcttcca acatatgaac aacttttgaa ctttgtgcgt
     2881 ttgctaaagg catcatagga aatcccatgg acatcaaaga agagatacca atattgaatc
     2941 tggaatgcac tggagagatg tgaaaagcga ttaatttctt ttccgaaatt tttgtgaaaa
     3001 ctgttttttg ccatttcgtc cgccatccca tagaatgagc agaatgacaa ataagtttag
     3061 ggataccagt tgtaccagaa gtatgggtca tataagaaat ttcatttttg tctaaaaatg
     3121 attgagaaac agacaaagca ggttgttcca ttaaataaag aataggaa
//
LOCUS       DAZUKC010000157         2999 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000157 DAZUKC010000000
VERSION     DAZUKC010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.164"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            155..1051
                     /locus_tag="ACG80T_002733"
     CDS             155..1051
                     /locus_tag="ACG80T_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFS4842335.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIVHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMLERIIQLENEKE"
     gene            1143..1358
                     /locus_tag="ACG80T_002734"
     CDS             1143..1358
                     /locus_tag="ACG80T_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFS4842336.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            1376..1648
                     /locus_tag="ACG80T_002735"
     CDS             1376..1648
                     /locus_tag="ACG80T_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFS4842337.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILTKEKVA"
     gene            1650..2513
                     /locus_tag="ACG80T_002736"
     CDS             1650..2513
                     /locus_tag="ACG80T_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFS4842338.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELVKLYEKDVVKYVTPYSNRMTEAI
                     ISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTP
                     PIPKTPKLPGI"
BASE COUNT         1182 a          479 c          520 g          818 t
ORIGIN      
        1 gataaataga ggtctttcca ctactatcta aatggaaaga cctctatttt tcatatagga
       61 gctacttttg ggtcagccct atatattaat cacaaatcac ttatcacaaa tcacaagtga
      121 ttaatcacaa atcacttgtg atttgtgatt cttaatgata caatattact atacaaaaaa
      181 agaatggggc gtagttatgg agaaggaaaa actaaaaata cttgaagaat taagacgtat
      241 tttaaacaat aaaaatgaag caattattat cttgaataat tactttaaag gtggtgttgg
      301 aaagtccaaa ttatcgacta tgtttgctta cttgacagac aaattgaatt taaaagtttt
      361 aatgatcgat aaggacttac aggcaacatt gacaaaagac ttagcaaaaa cttttgaggt
      421 agaattgcca cgtgtcaatt tttatgaagg cttgaaaaat ggaaacttgg cttcttctat
      481 tgttcatttg actgataatt tagacttgat ccctggcacg tttgatttga tgttactgcc
      541 aaaattaact cgctcatgga cttttgaaaa tgaaagtaga ttgcttgcta ctcttttagc
      601 acctttaaaa agtgactatg atctcattat tattgatact gtaccaacgc caagcgttta
      661 tacaaataat gcaatcgtgg cgagtgatta cgttatgatc cctttacaag cagaagaaga
      721 aagtacaaac aacattcaaa actatatttc ctatttgatt gatttacaag aacaatttaa
      781 ccctggacta gatatgatcg gttttgttcc ttatttagtt gatacggaca gcgcaacgat
      841 aaaatcaaac ctggaagaac tgtacaagca acataaagaa gataacttgg ttttccaaaa
      901 tattatcaag cgaagtaata aagtaagtac ctggtctaaa aatggaatta cagaacacaa
      961 aggctatgac aaaaaagttt tatccatgta tgagaacgta ttttttgaaa tgcttgagcg
     1021 aattattcaa ttagaaaacg aaaaagaata gaatcacaaa tcacaagtga ttaatcacaa
     1081 atcacttgtg atttgtgatt gttgatgata aaataagaat aagaagaaat agaaagaagt
     1141 gagtgattgt gggaaattta ggcgcacaaa aagcaaaacg aaatgataca ccaatcagtg
     1201 caaaaaaaga tataatggga gataagacgg ttcgtgttcg tgctgactta caccatatta
     1261 taaaaattga aacagcaaaa aatggcggaa acgtaaaaga agttatggat caagccttag
     1321 aagaatatat acggaaatat ttacctgata aactttaaaa aggagtgaaa atgaaatggc
     1381 agttacgtat gaaaaaacat ttgaaataga gatcattaac gaattatcgg caagcgttta
     1441 taatcgagta ttaaactatg ttttgaacca tgaactagat actaaaaata ctcgtttact
     1501 agaagtgaat cttttaaatc aattagaagt ggcacaagaa gttgatttat ttcaacaacc
     1561 atttgaagaa ttacaagcta ttcatgagta ttggcggtca atgaatcaat attcaaaaca
     1621 aattttgaca aaagagaaag tggcttaaca tggcgaatat tactgacttc accgaaaagc
     1681 aatttgaaga tcgtttagaa aagaatgttg aacgactaac taaaaataga ctagcggttg
     1741 aatcgccaac cgctttttta cttggtgggc aaccaggatc aggtaaaact agtttgcgat
     1801 cggcaatttc cgaagagaca caaggaaatg ttgttatcat tgataatgat acgttcaaac
     1861 aacagcaccc taattttgat gaactagtga aactttatga aaaagacgta gtaaaatacg
     1921 ttacccctta ttctaatcgc atgacagaag cgatcataag ccgtttgaga gataaagggt
     1981 ataatttagt gattgaaggt acaggacgaa caacagacgt tcctattcaa accgcaacaa
     2041 tgcttcaagc caaagattat gaaacaaaaa tgtatgtcat ggcagtacct aaaatcaact
     2101 catatttagg aacaattgaa cggtatgaaa ctatgtatgc agatgatcca atgacagcca
     2161 gggcaacacc aaaacaagcg catgatattg ttgtcaaaaa cttaccgacc aatttagaaa
     2221 cccttcataa aacgggctta tttagcgata taaggcttta caatagagaa ggagtgaaac
     2281 tctattcaag cttagaaacg ccttccatta gtccgaaaga gaccttagaa agagaattaa
     2341 atcgtaaagt atcagggaaa gaaattcaac cgactttgga gagaatagag caaaaaatgg
     2401 ttcaaaatca acaccaagaa acccctgaat ttaaagcaat tcaacaaaaa atggaaagct
     2461 tgcagccacc tacaccacca atacccaaaa cacctaaact tccagggatt taaaccttaa
     2521 aaaagtaaaa gagtagttac caaaaaacgg taactactct ttttttataa aaacatttcc
     2581 tcatttttta aaagctgatc gagaaataaa gcacacaact gtattctttc ttttttcagt
     2641 tggtacattt tgcagtctgt aacgtgccat ttactgtttg ttaccttaac atatccagtc
     2701 gaaaacacaa gggtatataa acgagcaagc tcgcccttgt gttttctttg tgtctatgtt
     2761 gcgttctcgt cacagcaaac gacacgaaca cactggcgaa aatgaaccaa caaaacgaaa
     2821 aaagaaagga gcgtacactg taatttattt ctagctttta aaacatgagg aaaacagctt
     2881 cacttcctca aacgcctaac aaaaatggtt gtcaaaaaac taaaaatcaa aaaaagaaaa
     2941 gaggaaaaga aaatggaaaa aacaaaaaaa ttacaattag aagattttac agaaaatgg
//
LOCUS       DAZUKC010000158         2954 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000158 DAZUKC010000000
VERSION     DAZUKC010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.165"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..695)
                     /locus_tag="ACG80T_002737"
     CDS             complement(<1..695)
                     /locus_tag="ACG80T_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4842339.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSL"
     gene            complement(815..1462)
                     /locus_tag="ACG80T_002738"
     CDS             complement(815..1462)
                     /locus_tag="ACG80T_002738"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFS4842340.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(1546..>2954)
                     /gene="uxaC"
                     /locus_tag="ACG80T_002739"
     CDS             complement(1546..>2954)
                     /gene="uxaC"
                     /locus_tag="ACG80T_002739"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFS4842341.1"
                     /translation="LLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQYKNLTQV
                     WLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPIFEWSHLE
                     LQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDDPTDDLWY
                     HTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGLKQALSQR
                     VDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQFVTGIQLH
                     LMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVALFDTAEK
                     SGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMRQQLTTMA
                     QQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYLSQIVENI
                     CYRNAYNYFNFFEIGSRK"
BASE COUNT          938 a          612 c          404 g         1000 t
ORIGIN      
        1 tttaaagatt ttaacaatga aactttgtat tgagattggt caattcttac tcgttcagta
       61 gttagtttat aacatattaa ataacaaata agtgcacata tcgaaaatat tcccgcaaca
      121 attgtaaaag ttgatccacc acgaactatt tgagcgcctg aatcatctct tgtataaata
      181 aataatggtg ttaatacacc aatgaccaaa cttgctaaag tagctccaac atttcggaat
      241 gtcgatagca ttgttctttc ttctgtaata gaagtaatag ctgatgccat tgatccataa
      301 ggaatattta tagatgtgta aaaaatactc ccccataata tatacgtaac atacatgtat
      361 actaccttaa cgctcataga agctgaaatc atagaagttt gatacattaa aaaacttgct
      421 aaagcgactg gaccactcat tcttaaaatc cacggtttaa acttaccctg cttagttgct
      481 ttcgatctat caacaatacg ccccatagtt acatcagtaa ctgcatcgat aaatcttgct
      541 actaaaaata atgttccaac ggcagctccg cttatcccca atacttttgt ataaaagatc
      601 attaaaaaag aactagcaaa aatgaaagta aaatcgtttc caaaatctcc aaacatatat
      661 cctattttgt ctcttatacc aaattttctt tccatacaat tctctccctt ctgttgttaa
      721 gcgttttcat tctgatgaaa gtacgtacta tactctgaag cgtaaagagt cagtatttat
      781 tttttacatt tcagagtatt atttgttttt tctcctaata attctttatc tcataaaact
      841 tatctatata tttactagct actcgactta cttcttcaaa atcacctttt tcaacagaag
      901 ccaataaatt cccacctaca ccaactgcaa caactcctac ttcaaaccaa tcttctaagt
      961 tctctatact tactccgcca gtaggcatta tatttacgtg tggtaatgga gcttgatagg
     1021 cttttacaat ggatggacca aaagcagaac ctgggaatag tttaatgata tctacgccag
     1081 cagtaagtgc ctcctttatc tcagtaattg tcatacaacc tggtaaataa ggaatttgat
     1141 ataaattaca aatctcagct gtttgtcgat caaaagatgg actaacgata tattctgccc
     1201 cagccataat tgcgatacga gcagtaaacg catctaaaac cgtaccggct ccgattatta
     1261 catctttttg atcagcatat tttttagcaa tttctttgat tatttcttca gcattaggaa
     1321 ctgtaaaagt tagttcaatc cctttaattc cacccgcaat aattgaattt attgctttaa
     1381 tggcctcatc cttatttttc ccacgtacaa ccgcaatgac tccagccgct tctaacttac
     1441 ttaaaatttc tacttttttc atatctatct ccttccttta tggatacata ctttgtctag
     1501 ctgtactaac ttacaaagaa taaatttaag cttttatttc aagtattatt ttctacttcc
     1561 gatttcaaaa aaattaaaat aattatacgc attacgataa caaatattct ccactatctg
     1621 actaagatac atttcatcat ctggaattcg gcctgcttta acccactcgc ttagtaattg
     1681 acataatact cgacgaaaat actcatggcg aggatagctt agaaaacttc gagaatcagt
     1741 caacatacct gtaaaattcg acaacaaact ttgttgtgcc atcgtagtca actgttgacg
     1801 cattccttca gctgtatcat tgaaccacca agcacatccc aattggattt tttgaatcat
     1861 acccccttgg aagctttgca ttgctgttgc tagtggaagc caatcagttg gatttaatga
     1921 atataaaatt gtttttggta agcacccact cttttcagcg gtatcgaaca atgctacaac
     1981 attttgtgtc aaactagctt gatcccccat cgaatcacca ccgacatcta ctccaatttg
     2041 ttcctttaat atacttgaat tatttcttaa agcatttaaa tgcatttgca tcgtccaacc
     2101 atatcttgta tactcagaca tcaaatgtaa ttgaatacct gtaacaaatt gtgcaatttc
     2161 tgataaatgg aaatccgttt ttcctgctaa agcttttatt aacagattgt cgagtatttt
     2221 ttcatctgct atttcataaa aataagtatt caaaccgtga tctgctaatc gacagccaat
     2281 ttgatgaaaa tgatccactc gttggctcaa tgcttgcttc aacccattaa agtctttgat
     2341 tggtatagcc gctgtttgac taagttttaa aatatagttc gaaaagcctg atgatttgat
     2401 actccagacc ttatctggac ggaaagtagg taataccttg aaagaattat cctgtttaga
     2461 aagctcagta tgataccaca agtcatctgt aggatcatcc gttgtacaga tggctttcac
     2521 atccatcatc tccaacaatt ctttcgggcg ataggaatcc gtttgaattt tttcgttagc
     2581 acgtcgccaa atttctggtg cattcttctc agaaattgtc aaatcaatat caaagtaacg
     2641 ttgtaattcc aaatgagacc attcaaaaat aggattacct ggcgccttct ccactgtttt
     2701 tacaaaagct aaaaattttt cataatcatc acctggacca ctgatcaact cttcttctat
     2761 accattagct cgcatcaatc gccatttata atgatctcca gctcctttat catttaacca
     2821 tacctgagtc aaattcttgt attgcttatt ttctagcaaa tatgctgggt ctaaatgaca
     2881 atgatagtcg ataattggtt ggtgttttgc aaatttatga aaaagtcttt tagctatttc
     2941 attctctaat aaaa
//
LOCUS       DAZUKC010000159         2927 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000159 DAZUKC010000000
VERSION     DAZUKC010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.166"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            226..1194
                     /locus_tag="ACG80T_002740"
     CDS             226..1194
                     /locus_tag="ACG80T_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFS4842342.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1187..2218
                     /gene="vanA"
                     /locus_tag="ACG80T_002741"
     CDS             1187..2218
                     /gene="vanA"
                     /locus_tag="ACG80T_002741"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFS4842343.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2224..2832
                     /locus_tag="ACG80T_002742"
     CDS             2224..2832
                     /locus_tag="ACG80T_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFS4842344.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          865 a          560 c          713 g          789 t
ORIGIN      
        1 ggaggtccta agagatgtat ataatttttt aggaaaatct caaggttatc tttacttttt
       61 cttaggaaat taacaattta atattaagaa acggctcgtt cttacacggt agacttaata
      121 ccgtaagaac gagccgtttt cgttcttcag agaaagattt gacaagatta ccattggcat
      181 ccccgtttta tttggtgcct ttcacagaaa gggttggtct taattatgaa taacatcggc
      241 attactgttt atggatgtga gcaggatgag gcagatgcat tccatgctct ttcgcctcgc
      301 tttggcgtta tggcaacgat aattaacgcc aacgtgtcgg aatccaacgc caaatccgcg
      361 cctttcaatc aatgtatcag tgtgggacat aaatcagaga tttccgcctc tattcttctt
      421 gcgctgaaga gagccggtgt gaaatatatt tctacccgaa gcatcggctg caatcatata
      481 gatacaactg ctgctaagag aatgggcatc actgtcgaca atgtggcgta ctcgccggat
      541 agcgttgccg attatactat gatgctaatt cttatggcag tacgcaacgt aaaatcgatt
      601 gtgcgctctg tggaaaaaca tgatttcagg ttggacagcg accgtggcaa ggtactcagc
      661 gacatgacag ttggtgtggt gggaacgggc cagataggca aagcggttat tgagcggctg
      721 cgaggatttg gatgtaaagt gttggcttat agtcgcagcc gaagtataga ggtaaactat
      781 gtaccgtttg atgagttgct gcaaaatagc gatatcgtta cgcttcatgt gccgctcaat
      841 acggatacgc actatattat cagccacgaa caaatacaga gaatgaagca aggagcattt
      901 cttatcaata ctgggcgcgg tccacttgta gatacctatg agttggttaa agcattagaa
      961 aacgggaaac tgggcggtgc cgcattggat gtattggaag gagaggaaga gtttttctac
     1021 tctgattgca cccaaaaacc aattgataat caatttttac ttaaacttca aagaatgcct
     1081 aacgtgataa tcacaccgca tacggcctat tataccgagc aagcgttgcg tgataccgtt
     1141 gaaaaaacca ttaaaaactg tttggatttt gaaaggagac aggagcatga atagaataaa
     1201 agttgcaata ctgtttgggg gttgctcaga ggagcatgac gtatcggtaa aatctgcaat
     1261 agagatagcc gctaacatta ataaagaaaa atacgagccg ttatacattg gaattacgaa
     1321 atctggtgta tggaaaatgt gcgaaaaacc ttgcgcggaa tgggaaaacg acaattgcta
     1381 ttcagctgta ctctcgccgg ataaaaaaat gcacggatta cttgttaaaa agaaccatga
     1441 atatgaaatc aaccatgttg atgtagcatt ttcagctttg catggcaagt caggtgaaga
     1501 tggatccata caaggtctgt ttgaattgtc cggtatccct tttgtaggct gcgatattca
     1561 aagctcagca atttgtatgg acaaatcgtt gacatacatc gttgcgaaaa atgctgggat
     1621 agctactccc gccttttggg ttattaataa agatgatagg ccggtggcag ctacgtttac
     1681 ctatcctgtt tttgttaagc cggcgcgttc aggctcatcc ttcggtgtga aaaaagtcaa
     1741 tagcgcggac gaattggact acgcaattga atcggcaaga caatatgaca gcaaaatctt
     1801 aattgagcag gctgtttcgg gctgtgaggt cggttgtgcg gtattgggaa acagtgccgc
     1861 gttagttgtt ggcgaggtgg accaaatcag gctgcagtac ggaatctttc gtattcatca
     1921 ggaagtcgag ccggaaaaag gctctgaaaa cgcagttata accgttcccg cagacctttc
     1981 agcagaggag cgaggacgga tacaggaaac ggcaaaaaaa atatataaag cgctcggctg
     2041 tagaggtcta gcccgtgtgg atatgttttt acaagataac ggccgcattg tactgaacga
     2101 agtcaatact ctgcccggtt tcacgtcata cagtcgttat ccccgtatga tggccgctgc
     2161 aggtattgca cttcccgaac tgattgaccg cttgatcgta ttagcgttaa aggggtgata
     2221 agcatggaaa taggatttac ttttttagat gaaatagtac acggtgttcg ttgggacgct
     2281 aaatatgcca cttgggataa tttcaccgga aaaccggttg acggttatga agtaaatcgc
     2341 attgtaggga catacgagtt ggctgaatcg cttttgaagg caaaagaact ggctgctacc
     2401 caagggtacg gattgcttct atgggacggt taccgtccta agcgtgctgt aaactgtttt
     2461 atgcaatggg ctgcacagcc ggaaaataac ctgacaaagg aaagttatta tcccaatatt
     2521 gaccgaactg agatgatttc aaaaggatac gtggcttcaa aatcaagcca tagccgcggc
     2581 agtgccattg atcttacgct ttatcgatta gacacgggtg agcttgtacc aatggggagc
     2641 cgatttgatt ttatggatga acgctctcat catgcggcaa atggaatatc atgcaatgaa
     2701 gcgcaaaatc gcagacgttt gcgctccatc atggaaaaca gtgggtttga agcatatagc
     2761 ctcgaatggt ggcactatgt attaagagac gaaccatacc ccaatagcta ttttgatttc
     2821 cccgttaaat aaacttttaa ccgttgcacg gacaaactat ataagctaac tctttcggca
     2881 ggaaacccga cgtatgtaac tggttcttag ggaatttata tatagta
//
LOCUS       DAZUKC010000160         2883 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000160 DAZUKC010000000
VERSION     DAZUKC010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.167"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(173..1030)
                     /locus_tag="ACG80T_002743"
     CDS             complement(173..1030)
                     /locus_tag="ACG80T_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFS4842345.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(1316..1602)
                     /locus_tag="ACG80T_002744"
                     /pseudo
     CDS             complement(1316..1602)
                     /locus_tag="ACG80T_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1592..1921)
                     /locus_tag="ACG80T_002745"
     CDS             complement(1592..1921)
                     /locus_tag="ACG80T_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS4842346.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(1998..2270)
                     /locus_tag="ACG80T_002746"
     CDS             complement(1998..2270)
                     /locus_tag="ACG80T_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFS4842347.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(2270..2533)
                     /locus_tag="ACG80T_002747"
     CDS             complement(2270..2533)
                     /locus_tag="ACG80T_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS4842348.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            2767..>2883
                     /locus_tag="ACG80T_002748"
     CDS             2767..>2883
                     /locus_tag="ACG80T_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFS4842349.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNR"
BASE COUNT          876 a          570 c          385 g         1052 t
ORIGIN      
        1 tctgtctttc aacattggag gaagttttta tttcttgaac ttggtgcact tctaattgct
       61 tattttcaga tggtgtaatt gcttgctgct ttttctttgc ttctgcttct ttttttattt
      121 tggcgagtcc tttgttgttc ccaaaaatat caagtccttt gatgtcagcc aattactcca
      181 cctccgcata ccaattctcg tgttcttcca attctttcaa aatactgata aataattcgt
      241 ggcttttgcg atcccattgt tttaagagac ctttctttgt cttctccatc gtaatacctg
      301 atccgtcgta tcgttttaag cgcgcatttt tgagtacaac atttttaatc aaatgttccc
      361 cataaatctc ttgggcttgt ttatacattt cctgatctac tgcgtcaccg ctttccaaca
      421 tgaaaggaat aacaccagca acatataaat ttgtatcaaa ttctttcaca aaaaagtcca
      481 tatactcaag atattgcatc gcatttctca tcgattttac ttgggtctgt aagacgacga
      541 ttgcccaatc cgcaatgtac atcgcggatt tactatatac agaaatagaa ggtgggacat
      601 ctaaatagat gacgtcaaac tcatcaaata atggttctag cttttctttc atatatttga
      661 attgggataa tgaatcatta tattggtctt tgatccaatc atcatacatt tcaaaggaca
      721 tgtctgctgg aagtatatag aggttattga ttactttata gacagcctct ttaggtggaa
      781 gattgtttat caacacgtca gtcacagtat tttcgtaaac aatcggttca ccttctccgc
      841 ctccttcttc gttagtgaag ttcatatgcg ttaagttcat cacatcagaa gtatttcctt
      901 gcatatccaa gtcaattatc aaggttcttt ttccttcatt aactgctgat tcaaacccta
      961 acatttgcgc gcacgtactt ttacctacgc cacctttaaa attaccgatt acatatttct
     1021 tcgctaccac gttcctacct cttttctttt agcaatattc atttgatact attataacat
     1081 atttattttt agttactacc aaaaaaaaga tatatatgta ctaccttata attaataagt
     1141 aaaaggtagt acataaataa ggaaaagctg aaatatattg atttatagct ttcctcaaag
     1201 taatgacgtt atttaattta tcgagaagca gagaatactc gcgactttag tcatgtagac
     1261 gaatcagatt ttcgataaaa aagaagccgt ccttcttaag aacgacaaca aatcactaga
     1321 ttaatttttt caactgttca taaaagtttt catgcgttcc acataacaat actggaataa
     1381 ccttgttttc tataatctcg taagctactc tatatgttgt gcccgcatat ttgaacccac
     1441 tagaccaaat acctgataaa tccccagttt tttgttcacc cttatatgga tcaatcgcaa
     1501 tttcatcata aattaaattc aaaaattttt ctttcaatgg cttctctttt agctttttaa
     1561 atacttaatg acatgttttg aaggaacgat ttcataagcc aatttctgtt gccaactcct
     1621 ctttagtcat tactttagaa ttagtaagtg tatcttcagc aatatctcta aaggaagaat
     1681 gcattttgtt ttttctattc ttaaattcta caagaatctc atctttatcg tagccttctg
     1741 caattatatc agacaaaata tcttcgctaa aatcataaaa ttctttttgt ggttcaacaa
     1801 attcataaag aataccattc tctaccaact ttgcttcaac ttcgactcca ttaggaacat
     1861 taaaatcttt aggtactgtc aacatgattg aattgccttg ttttcttgtt tttgtaatca
     1921 tttaaatccc tcatttctaa cagtatgtaa ttacttagta aatacattat accacgagat
     1981 aatgtattta caagtaatta ctgtgactcg taatgcgtcc ttgctgaata aatttcaacc
     2041 acgtgttcat cttcatctac cttataaaca acccggtgtt gtctattcaa tcttctagaa
     2101 tacctacctt catgtgtagg ttttaacttc tcaaatgatt gggtagggag atagggatct
     2161 tcctttaaag tctgaataac cttctcaaat tgaggtttca aattagactg tttgattttc
     2221 tttaaatcag ctttagccga gtttttaatc atcacagaat agttattcat tataagttat
     2281 cccaatcaat ttcatcaaca ttagtaaatc cactatcatc tttttctcgc ttacgaacaa
     2341 cacccaaagt gcctatttgc tcaagtaata gcgtttcttt gattgctttc caatcatcca
     2401 aaccaataag caccgcatta ttttcactgc gctcactaat gatttcaatt tccgtatgat
     2461 tctcatttac atctttcaac aacttataaa aatttttccg tgctgagcta ggatttaaga
     2521 ctgtttttgc catatctact ccccctctaa tttatatctc cattctaacg tacttaatta
     2581 agtacgtaaa ttcgattgtt ttgtttaaat tggagtacca aaacagcatt gtaaagtgtt
     2641 atccgtttcc tttatacccg tggtgctagt taattttttc ctagaggaaa atgtctaaac
     2701 atgttggtaa atcaacaaaa aaacgcatag agtagtctca aaactcccaa gaaatgagga
     2761 ctacatatgc gttcatcaaa atatactgaa cattatacag atacttttat aacattcaag
     2821 gaaattatga gaactgtttc taacatatac cacaattgtg taccagataa gattaaaaac
     2881 cga
//
LOCUS       DAZUKC010000161         2855 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000161 DAZUKC010000000
VERSION     DAZUKC010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.168"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            56..1534
                     /locus_tag="ACG80T_002749"
     CDS             56..1534
                     /locus_tag="ACG80T_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFS4842350.1"
                     /translation="MKKLSQKRLAQIIIDSRKSLKMTQQQLAVATGINRALISRLESL
                     DFIPSIQQLESLAEVLNFEPTDLFINESNKSNRTKHQSKNIAVAGTGYVGLSIATLLA
                     QHNQVTAVDILPEKVKQINNKKSPIQDDYIEKYLAEKNLNLIATTDGAAAYREADFVV
                     IAAPTNYDSKQNYFDTSAVESVIKLVLEVNPKAIMVIKSTVPVGYTESIRKKYNTKNI
                     LFSPEFLRESKALYDNLYPSRIIVGTDQTDKTLREASHTFADLLQEGAIKKDIETLFM
                     GFTEAEAVKLFANTYLALRVSYFNELDTYAEMKGLNTQEIIHGVCLDPRIGTHYNNPS
                     FGYGGYCLPKDTKQLLTNYNDVPQNMISAIVDSNRTRKDFIADRVLELAGTYEANSSW
                     NAEKEKEVVIGVYRLTMKSNSDNFRQSSIQGVMKRIKAKGATIVIYEPTLENNTTFFG
                     SKVINNLDDFKNISHSIIANRYDSNLDDVKEKVYTRDLFQRD"
     gene            complement(1602..2693)
                     /locus_tag="ACG80T_002750"
     CDS             complement(1602..2693)
                     /locus_tag="ACG80T_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFS4842351.1"
                     /translation="MLELNTSLSNRTILITGAAGFIGSNLVLELLRTQSSIKIIGIDN
                     MNDYYDVSIKNYRLSEIETEAKNHPGSTWNFIKGSIADKATIDFIFDNYKPSIVVNLA
                     AQAGVRYSITNPDMYIESNIIGFYNILEACRHSYDNGESGVEHLVYASSSSVYGTNKK
                     IPYSTEDKVDNQVSLYAATKKSNESMAHAYSKLYNIPSTGLRFFTVYGPAGRPDMAYF
                     GFTDKLRAGDNIQIFNYGNCKRDFTYIDDIVEGVKRVMQCAPEKIDGEDGLPIPPYHI
                     YNIGNNTPENLLDFVDILQQELIAAGVLPEDYDFESHTELVPMQPGDVPVTYADTSAL
                     ERDFEFKPSTSLRDGLRKFAQWYKSFYEV"
BASE COUNT          997 a          512 c          456 g          890 t
ORIGIN      
        1 tgttatagaa gaaaaaattg tttgttatta ttagcaacaa aaaaaggagt gcatcatgaa
       61 aaagttatct caaaaacgtt tagctcaaat cattattgat tctagaaaat ctctgaaaat
      121 gacacaacaa caacttgcag tagctactgg tatcaaccgt gccttaatta gtcgattaga
      181 atctctcgat tttatcccgt caatacaaca attagaatca ttagcagaag ttctaaattt
      241 tgaaccaaca gatctattta taaatgaatc aaataaatca aacagaacaa aacatcagtc
      301 gaagaatatt gctgtagccg gtactggtta cgtaggtctt tccattgcta ctcttcttgc
      361 ccagcacaat caggtaacag cagtagacat tcttcctgaa aaagtaaaac aaattaacaa
      421 taagaaatct ccaatacaag acgattacat tgaaaaatat ctagctgaaa aaaatcttaa
      481 tttaattgca acgactgatg gtgcagccgc ctatagagaa gccgattttg ttgtcattgc
      541 tgctccaaca aattatgata gtaaacaaaa ttattttgat acttctgctg tagagtcagt
      601 aatcaaattg gtgttagaag tcaatccaaa ggcaataatg gtcatcaagt cgacggtacc
      661 cgtaggctat accgaaagta ttcgtaaaaa atacaatact aaaaatattc tattcagccc
      721 agaatttctc agagaatcta aggcattata tgacaatcta tatccttcgc gaattattgt
      781 cggcacggac caaactgaca aaactttacg agaagcctct cacacttttg cagatttact
      841 ccaagaagga gcaatcaaaa aagatatcga aactcttttc atgggattca cagaagctga
      901 agcagttaaa ttattcgcta atacttacct tgcacttcga gtatcttact tcaatgagct
      961 ggatacttat gcagaaatga aaggactaaa tacacaagaa atcattcacg gggtttgctt
     1021 agatccaaga attggcacac attataataa tccatctttt ggatatggag gctattgttt
     1081 accgaaagat acaaaacaac ttctcactaa ctataacgat gttcctcaga atatgataag
     1141 tgcaattgtc gatagtaatc gtacaagaaa agactttata gcagatcgag tgttagaact
     1201 tgctggcacg tatgaggcta acagttcgtg gaatgcagaa aaagaaaaag aggtagtcat
     1261 aggtgtatac cgtcttacaa tgaagagtaa ttcagacaac ttccgtcagt catctattca
     1321 aggagttatg aagcgaatta aagcaaaggg ggcaactatt gtaatttatg aaccaacact
     1381 tgaaaataac actacatttt ttggttcaaa agtaataaat aatttagatg attttaaaaa
     1441 tatcagtcac agtattattg ctaatcgtta cgatagtaat cttgatgatg taaaagaaaa
     1501 agtatatacc agagatttat ttcaacgaga ttaaaaaata acacctcaga tttgtagtgt
     1561 ctgaggtgtt tctataaact tttataagta aaaaaataca attatacttc ataaaaagac
     1621 ttataccact gcgcaaattt acgaagcccg tcacgcaaac ttgtacttgg tttaaattca
     1681 aaatctcgtt ctaacgcact agtatctgca taagtaactg gcacatctcc tggttgcatt
     1741 ggaactagtt cggtatgaga ttcaaagtcg taatcttctg gtagcacccc tgctgcaatt
     1801 agctcttgtt gtagaatatc tacaaaatct agtaggtttt ccggcgtatt gttaccaata
     1861 ttataaatat gatatggtgg gattggtagc ccatcttccc catcaatttt ctctggagcg
     1921 cactgcataa ccctctttac tccttcaaca atatcatcta tataggtaaa gtcacgcttg
     1981 caattcccat aattgaatat ttggatatta tcaccagcac gaagtttatc agtaaaacca
     2041 aaataagcca tatctggacg cccagcagga ccatatactg taaagaaacg taaccctgta
     2101 gacggaatat tgtaaagttt agaatatgca tgtgccattg attcattact tttcttagtt
     2161 gctgcataca acgatacttg attgtctact ttatcctctg tagaataagg aattttctta
     2221 ttagtaccat aaacggaaga cgatgaggca tacactaaat gttccacacc agattctcca
     2281 ttatcatatg aatgacgaca agcttcaaga atattataga atcctattat gttagattcg
     2341 atatacatat ctggattagt aattgaataa cggactcctg cctgagctgc taaattcacc
     2401 acgatacttg gtttataatt atcaaagatg aaatcaattg ttgctttatc agcaatagaa
     2461 cctttgataa aattccaagt agatcctgga tggttctttg cctccgtttc tatctctgat
     2521 aatctatagt tcttaataga gacatcatag taatcattca tattgtcaat gccaatgatt
     2581 tttattgatg actgagtacg taataactca agaaccaaat ttgatccaat aaaaccggct
     2641 gcgccagtta ttaatattgt tctatttgat aaggacgtgt ttaattccag cattttttag
     2701 cactcacttt ctttttttct tatgaataca tatttgttat atattaaaaa atcttattta
     2761 gacttatcta ttgtcacttt tatcattcta atctttgcac gtagtagaat aattatacag
     2821 ataggaaatt gctaagcaac ccacttcttt ctatt
//
LOCUS       DAZUKC010000162         2814 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000162 DAZUKC010000000
VERSION     DAZUKC010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.169"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            121..>2814
                     /locus_tag="ACG80T_002751"
     CDS             121..>2814
                     /locus_tag="ACG80T_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4842352.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
BASE COUNT          928 a          444 c          587 g          855 t
ORIGIN      
        1 caatatttgt agctatgaaa aaacataaaa atcatattag ataaaaacta aattggtaga
       61 taaattattt tgtcagatat ctgaggcaaa tattcgacta tttgtgtttc aaagtaaaaa
      121 ttggaataca aatatagaat agacatattg cagccgatag tttattatgc ttttagacac
      181 attaaactgg aggaaaaaat gaaaaagagc gctaaagatc gagtttttga tgagctgata
      241 cgactaacag ggaatatgtc ttctgtcact gccgagaaaa tttcgaagaa tctaggtatc
      301 tctcgtcaga atgccagcca ttatttgacg agattagtgg aagataaaaa ggtcgagaaa
      361 ttaccgggaa agcctgtttt gtggaagcct ttagatgaat atgcggttgt agataatacg
      421 gagcaaataa acgaagcctt tcattctgta gttggtcatg acggaagtct gagagaggtc
      481 attcaaaaat gtatagccgc tgtcaaatat ccgcctaacg gactcagtgt attaataaat
      541 ggagccacgg gtgttggaaa aagtttttta gccacaaaaa tttttgaata cgctatccat
      601 gagcaaatta ttgaaaaaga tgcaccattt gctatcttaa actgtgccga ttatgcggac
      661 aatcccgaac tgctttcggc aacactgttt ggatacaaga agggggcctt tacgggggct
      721 gaaaaggata ccgatggttt attggcaacg gccaacaacg gctatctttt tctcgatgag
      781 gtccatcgcc tatcgaaaga aaatcaagaa aagctctttc tatttattga tactggaaac
      841 tatcgtccag tcggtgaaaa tgtaaattgg cattctgcta aggttcgttt tattttcgcc
      901 acgacggaaa aaggtgaaaa ttatttatta gacacctttg atcggcgtat tcaaattagt
      961 gttatgttgc caacttttga agatcgcccc attcgagaac gattagagct gattcaatta
     1021 ttttttcaaa atgaagcgga tgttttacaa aaagatatta ttgtttcaag agaagccttg
     1081 tctttaatgt tatctcatcc ctttagtggg aatattggta agctgaagaa tatcattaaa
     1141 atcagttgtg cggatgtgta tagtcgaaca caagaagaac cattaacgat tgggaaaaat
     1201 gagatcttga ttcagttaaa tatgctggaa tccgaagtag aaagcttgcc gattgctagt
     1261 ttattgataa agaaaaatca aaagataaaa acggatcgaa tcaaaccgtt tgattatgat
     1321 tcgattttgg aaaaaattac tcagctgata aatgaaggta atttcaatga aaatttttct
     1381 tcgataaaga atgaagtaca gcatttggtg catcacattg ataaggattc ttttttatca
     1441 atgagtagaa aattgatgat tcagatgttt agaaaggtct gggtatccgt tttaggaaaa
     1501 acgtacggat tgacgacgag tggacctgtt gcagaaatta ttagcagatc ctatgtcatt
     1561 tccccaagct ataagacaga cttaaccggg ataaatcgta cattagctgc aaatctacct
     1621 cggacgactt acttatgtaa tcagttcttg gagcatttac caccgctctc agaaaaggac
     1681 gagaactttc taagactgct tattgtcgtg actttatctg attatgtaga tgaatccatc
     1741 gagctgaagg gcctgttgct tgctcacgga gatagtacgg ctagcagcat tcaagcagta
     1801 gtgaatcaat tatgcggcaa ttatgttttt gaagcgttgg atatgccagt agagagtaat
     1861 gtgtcagaga ttgtcgaatc gacaaggaat ttcgtgaaac ggcaagtgaa tacagatagt
     1921 ctagtgctga tcgtagatat gggctcttta aatcaaattt acacacagat caagaacgat
     1981 ttaaaagggg agttacttct tctgaataat ttgacgactt ccattgcact tgatattggc
     2041 ttgaagatga cgatgaatgc gccatttaaa cagattgcgg aggatgcagc agacaagtat
     2101 tcaatcaatg ttcaatactt tgaaggcttt tctcaaagta gcaacattat catttcctgt
     2161 atgtcgggtt tggggatttc ggacaaattg aaagatgttt ttcgtcatca tttatcggat
     2221 gaatccatcg aagttttcac gaaggactac agagaactac gtgaattaat cgaccaaaat
     2281 gatgaaactt attttgacaa gacaaaattg gtcatcacga cttcggatct tccacgttcc
     2341 ttttcaattc ctaatattaa tgtttatgac atcttagacg gtgatggagc agccaatttg
     2401 gagcatattt tgagtcctga aatgaagccg gcagaattta accgcttgat tcaggagcta
     2461 gtaaaattct tttcgttgga aggaattgcg gaccgtttga actttctgaa cccaacgatc
     2521 gtgatgagtg aagtggaaac agtcatttct aaatatgaaa attactatca tttcacttta
     2581 aatggccgtg taaaactaaa tctctatatg catacggcat tgatgatgga acgtttattc
     2641 ttggctcgga cagaaaaatc tgaagaaccg aattatgatt tgaaaccaga tgaagatgaa
     2701 ttttattatg tttctagaag tatttttcaa tcaatggaga tgaaatataa ttttaaagtc
     2761 aatgcttatg agctttcgct gctctatgaa ttattaggac cgtatattca aaaa
//
LOCUS       DAZUKC010000163         2720 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000163 DAZUKC010000000
VERSION     DAZUKC010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.170"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(75..1169)
                     /locus_tag="ACG80T_002752"
     CDS             complement(75..1169)
                     /locus_tag="ACG80T_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFS4842353.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1358..2119
                     /locus_tag="ACG80T_002753"
     CDS             1358..2119
                     /locus_tag="ACG80T_002753"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4842354.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2234..2674)
                     /gene="fabZ"
                     /locus_tag="ACG80T_002754"
     CDS             complement(2234..2674)
                     /gene="fabZ"
                     /locus_tag="ACG80T_002754"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFS4842355.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT          859 a          545 c          464 g          852 t
ORIGIN      
        1 ctataaaaaa acaaaataaa aaagcagaaa taactggcta tttctagcgg attatttctg
       61 caataactaa ttatctattt tttattttca attaatttaa agcttgacca tggtttcaaa
      121 aagtcaagga ggaataattc gtcatcggag attcttccta caacattgac acgtccgtcg
      181 tttttcatat ccttcaaggc gatttgtgtt tctcctttgt attgaccgta cccttcattg
      241 tcaatcagta catccccatg atgcatatct cgcgtattat gaggcgggaa atctttatct
      301 ttgtagtata cacgagtcat cgttgaacga aggatatatt ctgagcgatc ccctcgataa
      361 ctatgaaggt tatcaaacaa gcagattcgt tcgttttctg taataccttc ttccgtatct
      421 actttcaatg ttggataatc cgcgttaaat gcttctgcca tttctttcaa ttcttcttca
      481 cttgcgtacg catttccaat agaaatatca tcaatcaatc ctgtaaggat taaatgcttc
      541 acttgtgtcg caatttcaag atctcggtgg tcttccagcg tgcacaagcc atcttgcgta
      601 ggccaagggc caaactcagc agattgcgaa ttgacgaatg ccatcgtatt caaattatat
      661 tttctgaatt tttctgaaca aaaaacaaag tgttcgtatc ctaaaccaga gtatcgatga
      721 ggataaaaat tatgagaacc taataaatta tccgtgtttg gcgaataact catgatgttg
      781 tcaacgtaat tcgtgcccga actcatattg atctcgattt tgataccaaa aggattgcgc
      841 gtcatttttg cttcttctgc tcctgtgaat ccgatatcta agcggacccc gtaggcaccc
      901 atcttatgga aaaaagataa gtcatcgtaa gaaatctcca actgttcaaa taacgctgga
      961 ttgatatcaa ccattacttc catgcctagg ctattcgcgt aatcaactac ttctttaaat
     1021 tctgaaagaa ctttttcttt atcatcattg atctgcagca aactggtaaa aacccgttta
     1081 tacccatact tatgagctaa atccaagtac gctttgtcct tttcaaatgt tgaccgttct
     1141 ggataaatcg agattcctaa ttttcccata ttaccatttc ctcctgcatc ttttcgtttt
     1201 tgtatccact tacattatag caaacacaaa gtaaatggtc tacacctttt ctttttcttt
     1261 taaaataaat gataaattcc aataatttaa gtaggtattt tccctctatt ttgttatcat
     1321 taaagataac aaaatagtaa attggaaagg aacagttatg aagaaaaata aagttatttt
     1381 aagtattagt ttagcagcag cattgctgtt tgctgctggt tgttctgcaa acaacacgac
     1441 ttcttctagt tcgtctacag aaagttcaaa aagtacatct actgcggaaa gcacgactgc
     1501 cagtagcgtc gatttgaacg cattggaatt accacaattg acgaaagatg tcgccgaaaa
     1561 tgaagatctg gtcgaaatgg tgacgaataa aggcacaatc aaaatcaaat tatttcccga
     1621 acacgcacca aaagcggttg aaaactttat gacacatgca aaagacggtt actacaacgg
     1681 actgactttc catcgcgtca tcaaagattt catgatccaa ggtggcgatc ctaaaggcga
     1741 tggcactgga ggggaaagca tttggggaga aggatttgaa acagagatca acaaccacct
     1801 ttataatata agaggtgcgt tatctatggc tcgctcacaa gatcctaaca gtaacggcag
     1861 ccagttcttt atcgttcaaa atacagatga tatgcatgat ggtcttttaa aagacgacta
     1921 tcctcaagca atcatcgacg cttataaaaa cggcggttat ccttcactag atggccatta
     1981 tactgtcttc ggccaagtga ttgaaggaat ggatgtcgtg gatgctattg caaatatcga
     2041 aacagatagt tcagacaaac caaaagaaga agtaaaaatc gataaaatca atatactgca
     2101 agaagcaaaa caaaaataaa aaaataacgt ctgaatcctg catagatgac aacgtatgct
     2161 aggattcagg cgtcttcttt ttacataaaa agcaagtaag cttccttttg ttttgctata
     2221 tcaggtttta tagtcaagca ttttggttga cgatgaacgt gaactcgcac tcacagactt
     2281 ttttatcatc tacatatgct gcagataacg ctgtaccgac tgatcccttc attttggtga
     2341 tttcaaaatg acatttcagc acatctcctg gaacaacttt ttgacggaat ttggccttat
     2401 tgatcccacc gatataagct ctttttccta gaaactcttc tgattttagg ataagtatcg
     2461 aacctgcttg tgccagtgtt tctagaatca acgcacccgg catcactgga ttgtcaggaa
     2521 agtgaccttg gaaaaaatct tcattgattg ttacattttt tgtcgcaacg atcttttttc
     2581 ctggttctaa agtatctaca taatcaatat aacaaatagg ataacgatta ggaatcaatt
     2641 ccattacttc agtagcagat aatacgtaag acataattaa actccttctt tcaggcaatt
     2701 gccattttca ttactttgac
//
LOCUS       DAZUKC010000164         2639 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000164 DAZUKC010000000
VERSION     DAZUKC010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.171"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(76..1689)
                     /locus_tag="ACG80T_002755"
     CDS             complement(76..1689)
                     /locus_tag="ACG80T_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4842356.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNNYTSARILAGLGIKAILIEE"
     gene            complement(1830..>2639)
                     /locus_tag="ACG80T_002756"
     CDS             complement(1830..>2639)
                     /locus_tag="ACG80T_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4842357.1"
                     /translation="FRLLINESANLYAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKE
                     TQTFIIIQFYFDEQLLAHITGQSFSIDQEITSIITFLKNEQFKIDYLKHSKPAISYDQ
                     EINSFFILCQQMSIHLLSLLVTYCLQKIQLSSLPRHYQQELALLTIPSNEKVIPKKKH
                     ILYSDELVDPIIRYLEEHLSENFLLEQLAQIFFISPASLKKHFRKVNGKSVISYFKEL
                     KMEKAKRLVLENELTYTEIADILGFHSIHHFSSAFKKYTGLSPSKYYLSLK"
BASE COUNT          815 a          474 c          467 g          883 t
ORIGIN      
        1 cctatatact ttttcatttt tactcctcaa gtaaaaataa ctcctagacc gcggctaccc
       61 accgcggtcg tttttttatt cttcaatcaa aatcgctttg atacccaaac ctgccaagat
      121 tcgggcagaa gtataattat tcaatcctct tctgaaagta atgtaaactt ttttatcttt
      181 tgggatttca ttgattcgat ctcgtaactc attcattgga atattttttg ttgccgagat
      241 actgccagaa ggtgctttat taggttcacg tatatctaaa aagaacgctt ctttcaaatc
      301 gttttcgggt atatcacttg ctttgatcgt ttctacgata ttcgtcattt gattgattgc
      361 gacataacca gctatattca acgggtcacg ggtagtagaa tacggtggag agtaaggcaa
      421 ctccaaatca ggcaaatcaa agactgtcaa gtttccggtg attgctacag aaagttctcc
      481 catccgttta tcgacacctt tttctccaaa tgcttgtcca cctaaaatac gaccgctatc
      541 tgcatcaaaa atcagcttta gatttaatct tgtagcttct ggatagaaat aggcatggtc
      601 aaagggagtg ataaaaacag ttttataatt tgttatgcct gatgctttta attcatgttc
      661 tgtcattccc gcataacttg ctgtatgatc aaagattttt gcgacacctg caccaatata
      721 tccgcgatag cgcataggcg taccatttaa aatatctgcc agcatatgtc cttgacgatt
      781 ggccgcactg gaaagcatac tttggatcgg ctgaccagtc actacactag tcgtttcgat
      841 aatatcgcca atcgcataga tgtccggcag attggtttgt aactggtcat caacaatgat
      901 ctgacctgta tctgataatt gtatgccggt tgctttcacc acttcattct tcggagaaac
      961 accaacagca aaaattagca tatcagcaga aaggacggag ccatctgata atactatttc
     1021 tcgaccttta tcacggatct cagtaactct tgtttctaaa tgaacggcta atccttcttt
     1081 cagcaatttg tcataaacta aattagcaat ttctggatca taagggaaag cgacttgtgg
     1141 caattgatcg attaaagtca cgtctaagcc gcgatgcttg aaattctcag ctaattccag
     1201 acccataaca cctgcaccaa gaatgacgac atgttgtgga tttttttctt caaggaacga
     1261 tttgattcga tccgcatccg tcacactacg gaggacaaag ccgttttctg cttcaaaaac
     1321 tccgggaata tccggataat ctggtctagc accagaagag atgatcaact tatcgtaatc
     1381 tgtttttatt tcttcatttg tggataggtc tttaactgtt aatcgcttag tagaaggatc
     1441 gattgctgtt acttcgtgtt tagtaaagac atcgatgttg ttttttgttt tcaatatttc
     1501 tggtgtacgt tcgatcagcg agtcacggtc agtgatcaca cctcctaaat aataaggcaa
     1561 agcacaactt gcataagaaa tattttctcc gcgttcatag ataatgattt cgtgcgcttc
     1621 attcaatcga cggaaacgag tggcaaatga aggaccgcca gctacaccgc caatgacaac
     1681 aactttcata aaaacatctc cttttctacg ttgatcgtac tatgttatgg taaaaaacag
     1741 caaataattg tgccggaaaa ttattgttta ttttgaatag acagaaaata tagagaaata
     1801 atttgtggat aaaacctaaa aatttttttc tattttaagg atagataata ctttgaaggg
     1861 gataaccctg tatatttttt aaaagctgaa gaaaaatgat ggatcgaatg aaatcctaat
     1921 atatctgcaa tttctgtata ggttaattca ttttctagca cgagacgttt tgctttttcc
     1981 atttttagtt ctttaaaata gctaataaca ctttttccat tcacttttct aaaatgtttt
     2041 tttaagctgg ctggactgat aaaaaaaatt tgggctaatt gttcaagcaa aaaattttct
     2101 gaaagatgtt cttctaaata tcgtatgatt ggatcaacta attcatctga ataaagaata
     2161 tgcttttttt taggaatgac tttctcattt gaaggtattg tcaataaagc aagttcctgt
     2221 tgataatgcc ttggtaagga agacaattga attttttgca gacaataagt aactaataag
     2281 gaaagcaaat gaatgctcat ctgttggcat agtatgaaaa aagaattgat ttcctgatca
     2341 taggaaatag ctggcttact atgttttaga taatcaattt tgaactgttc atttttcaga
     2401 aaagtaatga tagatgttat ttcttgatct atagaaaaag attgtcccgt aatatgtgct
     2461 aataattgct catcaaaata aaactggata atgataaaag tttgtgtttc cttttgagta
     2521 gaaaagattt ggacttcttg ctgtttatca atgaataaaa gggaaggatt atctagttct
     2581 atagatttat gtgcaaattc agcatataaa ttagcgcttt catttattag tagtcgaaa
//
LOCUS       DAZUKC010000165         2557 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000165 DAZUKC010000000
VERSION     DAZUKC010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.172"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..719
                     /locus_tag="ACG80T_002757"
     CDS             <1..719
                     /locus_tag="ACG80T_002757"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4842358.1"
                     /translation="MKKEMYIMIKAVIFDLDDTLVSEYDYVKSGYKCVARRLAVQKIF
                     PATFDEIYRVLLDLFAEDSKNVFNRLYDYFEIPYSKKEIKELVNVYREHKPDINFLEN
                     ADTLLTELRSRGYKLGIITDGYAITQRNKLDALKVDYLVDYICITDELGRELWKPHPI
                     SYLLLLEKFNLKAQECIYIGDNELKDFITAKSIGMQTIKIERVNGIYRESSLKFKRFY
                     QADKKIRSLWEIKNFLGVIS"
     gene            716..1834
                     /locus_tag="ACG80T_002758"
     CDS             716..1834
                     /locus_tag="ACG80T_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HFS4842359.1"
                     /translation="MKSKRILLASPHMSEEGYEKEYVKEAFDTNWVAPLGANVNGFEK
                     ELAEYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPILYQGAIPV
                     FVDSDYETWNMKPEHLRTALEKYPNTKAVLVVHLYGLSANLDEITKICKEYDVTLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERIEKKRYIHSFYKKELSKLSNIQF
                     MPENNWNEPNYWLSVITIKGINANHLIDELDKNNIEARPVWKPMHLQPVFKNYDYIGD
                     DVAETLFDLGVCLPSDTKMTDEDLKYVVSTIKRVINDA"
     gene            1824..2444
                     /locus_tag="ACG80T_002759"
     CDS             1824..2444
                     /locus_tag="ACG80T_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFS4842360.1"
                     /translation="MMRKKTFYQLFGKRILDILLSGIALIVLSPIILIVGFLVRIKLG
                     SPIIFKQERPGKSEKIFSMYKFRTMTDERDHNGEYLPDEIRLTKFGKMLRATSLDELP
                     ELWNILKGDMSIVGPRPLLVEYLPLYSEKQRKRHNVRPGLTGLAQVNGRNAISWEEKF
                     DLDVYYVDKISFFNDLIIIIQTCKKVIKKENINTINNNIPEKFKGS"
BASE COUNT          968 a          322 c          494 g          773 t
ORIGIN      
        1 agatgaaaaa ggaaatgtat ataatgatta aagcggtgat ctttgattta gatgacactc
       61 ttgtgtctga atatgattat gtaaaaagtg gatacaaatg tgtagctaga agattagcag
      121 tgcaaaaaat tttcccggct acttttgacg aaatatatag agtcctttta gatctttttg
      181 ctgaagatag taaaaatgta tttaatcgtc tatatgacta ttttgaaatt ccttatagta
      241 aaaaagaaat taaagaactg gtaaatgtat atcgagaaca caaaccagac ataaattttt
      301 tagaaaatgc agatacttta ttaacggaat taagaagtag aggatacaag ctggggatta
      361 ttacagatgg gtatgctata acccaacgaa acaaattgga tgctttgaaa gtagactatt
      421 tggtagatta tatttgtatt actgacgaat taggccgtga attgtggaaa ccacacccta
      481 tctcatatct tttattgtta gaaaagttta atttaaaggc acaagagtgt atatatattg
      541 gcgataatga gttgaaagat tttataactg ccaaatcaat aggaatgcaa acaattaaaa
      601 tagaaagagt aaatggtatt tacagagaaa gcagtttgaa gttcaaaaga ttttaccaag
      661 ctgataaaaa aatacgttcg ttatgggaga taaaaaattt tttgggagtg atttcatgaa
      721 aagtaaaaga attctattag cttctccaca tatgagtgaa gaaggatatg aaaaagagta
      781 tgtaaaagaa gcatttgata caaattgggt tgctccttta ggtgcgaatg tgaatggatt
      841 tgagaaagaa ttagcagaat acgtgggagc tagtcatgct gccgcattat cttctggaac
      901 agcagctata catttagctt tgaaagcggc tggggtcggt aaagatgaca tagtgatttg
      961 tcagtccttg acgttctcag ctactgctaa tccaattctc tatcaaggag ctatacctgt
     1021 atttgttgat agtgattacg aaacttggaa tatgaaacct gaacatttgc gaacagcact
     1081 agaaaaatat cctaacacaa aggctgtatt agtagtacat ttgtacggtt tatcagctaa
     1141 tttagacgaa attactaaaa tatgcaaaga atacgatgtt actctaattg aagatgctgc
     1201 agaatcatta ggaacaacat ataaaggaaa atatactgga acatttggcg actatggaat
     1261 ttattctttt aatggaaata agataatcac aacttcaggt ggcggaatgc ttgtatccaa
     1321 tgacaaagaa aaggtggaaa aagtgagatt ttgggctacg caatctcgtg agaaggcacg
     1381 ccattatcaa cacaatgaaa ttggctataa ttatcgaatg agcaatatcg ttgctggtat
     1441 cggtcgagga caattgaaag tattaaatga aagaattgag aaaaaacgat atattcatag
     1501 tttttataaa aaagaattgt ctaaattgtc aaatatacaa ttcatgccag aaaataattg
     1561 gaatgaaccg aattattggt taagtgtcat aacaataaag ggaataaatg cgaatcatct
     1621 tattgatgag ttagacaaaa ataatataga agcccgccca gtatggaaac ctatgcattt
     1681 acaacctgtt tttaaaaatt atgattatat tggcgatgat gtagcagaaa ctttatttga
     1741 cttaggagta tgtttaccat cagatacaaa gatgacagat gaagatttaa aatatgtagt
     1801 atctacgatt aaaagagtga tcaatgatgc gtaaaaaaac tttctatcag ctttttggga
     1861 aaagaatttt agatatccta ttgtcaggaa ttgctctcat cgttttatcg ccgataatct
     1921 taattgtagg ttttttagta agaattaaac ttggttctcc aattatcttc aaacaagaac
     1981 gaccaggaaa aagtgaaaaa attttttcca tgtataaatt tcgtacaatg actgacgaaa
     2041 gagaccacaa tggagaatat ttacctgatg aaattcgact aactaaattt gggaaaatgc
     2101 ttagagcaac aagtctggat gagctacccg aactttggaa tattttgaaa ggtgatatgt
     2161 cgatcgtagg acctcgtcca ttattagtgg agtatttgcc actatatagt gagaagcaac
     2221 ggaaaagaca caacgtaaga ccaggattaa ctggactggc tcaggtcaat ggaagaaatg
     2281 ctattagttg ggaagaaaag tttgatttag atgtttatta tgttgataaa attagtttct
     2341 ttaatgatct tattattata attcagacat gtaaaaaagt aatcaagaaa gagaatatta
     2401 atacaatcaa taacaatatc ccagaaaaat ttaaagggag ttaaatacag cagcttaatg
     2461 caagttttgt gacttgcatt atatttaggt ataaatcaaa gcgagtatat tagaattata
     2521 taacatatgt taaaaaaacg tatagctaat aaaagta
//
LOCUS       DAZUKC010000166         2491 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000166 DAZUKC010000000
VERSION     DAZUKC010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.173"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1335
                     /locus_tag="ACG80T_002760"
     CDS             <1..1335
                     /locus_tag="ACG80T_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842361.1"
                     /translation="IDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            1322..>2491
                     /locus_tag="ACG80T_002761"
     CDS             1322..>2491
                     /locus_tag="ACG80T_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFS4842362.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKN"
BASE COUNT          831 a          377 c          420 g          863 t
ORIGIN      
        1 atagatgctg cttttttatt tagttgttta tatacttttg caacttatcg aacagttgat
       61 atctattatc gatccgcaat tgcagaagca atcgctctta cgattatccc agctgtttta
      121 tattatgctt atggattgat attcaataga gaaaaaaaag tcatcgcttt atctgtcagt
      181 ataagtctac ttgtgtatac tcatgtctta actacactat tgtgtagtgc attgattgct
      241 tgtatgatta tagttcgttt gtttttaagc aagcaaataa aaaacgatat aaaatttgtg
      301 gtcgtgaatt tagtaaaggc tgtttttttg acttgtgcat taactagcta tttttggtat
      361 cctatgttgc agcagatgaa ttatcaagaa atacacagtc ccttcaaaac agatttgcag
      421 ctagaagcac tttctatttc agatagtatc attcaagcgt taaataacga tattacaaca
      481 tacactatgg ggttattagg attggttgcg ttgatttttc ctttattcat actgaaagaa
      541 atggagaaaa aagaaaaaat catctatgtt ggcgtagttt ctacatgggt atgcgtaact
      601 aatttatttc catggttttt gtttcaaaaa acaccgataa gcttactcca gtttccttgg
      661 cgtatcttag gtatccaaat ctttttttct tcaatgatca taacgcttgt ttttatgaaa
      721 aagactatga ataaaaaata ttcatggtta atcattggcg ctactgtggt gtttctactc
      781 gtcacgtcta tagcagcaaa agaaaattac gcaaaagtta tccaatcaaa acacggtcat
      841 attgttatca atgaagaaac actcccacgt tatacaacat cagatatggg aggattatac
      901 gattacgccc caacagaagc gataaaagaa aggcctcact tagaaaaaca tgaagtaaaa
      961 gtaggagaat cgtgggaaaa agggaagtat aaagctgcag ataataacat tacttacaca
     1021 gtggcaagca acaaaaagca aaaagtgaca gtaccagtat atgcttattt aggtacacag
     1081 gtaaaagtaa acggtaaagt cgtaaatgac tcttataaaa aacatggctt agtgaatgtg
     1141 gatgtaaatg ctggagaaaa taagatagaa attacaacaa aatatactcc aactgctctc
     1201 atggctttat ggatcagtat tctgacttat tgtttagtgg tgtattcaag ttttaaacga
     1261 tttgcaccta ataagttgaa agattctcga tatgtgacta aaaaggagat aaacaagaag
     1321 aatgaaaata aataaaaaag tagtaatatt gagtggatta attatagtta gtagtatata
     1381 ttctttcatc tttttcaaaa atacacagag tatttatacc actggagatc tttcttttca
     1441 cttatctcga atcaaggggt tatcgactat ttttaccagt ccgattaatt atgagacatt
     1501 caattatact ggatatggtg taaattattt ttatcccttt ctgacttttt ttccagctgt
     1561 aatgttatat tggatgacaa aaaatttgat tgtaagctat attatttacg tttggttatt
     1621 gaacttgtgt acaacactcg tagcttatca ttatggcgag cggtttttaa aacaaaaaaa
     1681 agcggcattt ttatttagtt gtctctatat tttctctgct tatcggacag tagacatcta
     1741 ctatcgttcc gcaattgccg aagctatcgc aataacactt gttatccctg tattatttta
     1801 tgcttatcaa atcatctctg gaaaagaaga aaagtatccg tctgtaaagc tagcattgag
     1861 tatgagtttg ctagtgtaca gccatgtact atcaacatta atgagtacag cattaattat
     1921 catttttatt tttattcgtt tggtttctaa aggtttcaag aatgcagatt ttattgctat
     1981 atttaaaaaa ttatttagtg cggcaggaat gacattggtt ctgacgagtg ctttttggta
     2041 tccgatgttt gaacaaatgc tctaccaaaa aatcaacaaa cctagcgtga caaatttata
     2101 tgctcatgca tctaatgtgt tcgatagtct gactgaagct atgaacaacg acttaactac
     2161 ttactcaatg ggactagttg gtctgttatc tttatgtatt cccctgattt tatttaaaaa
     2221 attaacaaac atagaaaaaa agatttatta tggtacgtgt ttgacttggc tagctacaac
     2281 tagtttggtg ccatggtatc ttctgcaaaa tacgccagcc aaattgttac aatttccttg
     2341 gcggatctta agcttgcaaa taatattctc ttctctgata ttaacgatga tatttttcaa
     2401 aaatagaaga tacaataaga caagagaact attttattta tttatgactg ttttgttatt
     2461 aatcacttta actgtttcta gtaaaaaaaa t
//
LOCUS       DAZUKC010000167         2452 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000167 DAZUKC010000000
VERSION     DAZUKC010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.174"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(11..295)
                     /locus_tag="ACG80T_002762"
     CDS             complement(11..295)
                     /locus_tag="ACG80T_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842363.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            complement(995..1090)
                     /locus_tag="ACG80T_002763"
     CDS             complement(995..1090)
                     /locus_tag="ACG80T_002763"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFS4842364.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            1765..>2452
                     /locus_tag="ACG80T_002764"
     CDS             1765..>2452
                     /locus_tag="ACG80T_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS4842365.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLP"
BASE COUNT          798 a          393 c          456 g          805 t
ORIGIN      
        1 tttctcatgg ttatatcatt cctccgataa gcatttcaat gatttgatta tttgtgtaga
       61 catactcgct taatatttga gtattttctg tttggatagc aataaattta tcgagcaata
      121 aatcgaattg gtcttttttg taagtcgtaa gttgacggtc aaaatcactt tcgattaaaa
      181 cttttagata tttatttcga ctccattttt ctcctgtttt attcgtgagt tcttggcaac
      241 gtttgtctat tgttgcaaca aactttggat caatattccg aactaaaatg tccataaaaa
      301 atctcctttt gcaattatgt ataatttatg tataccatac aaatactata tttgtatagc
      361 tgttttttgc caaaatgcta tacataagct acccacgatg gcgaaaaagt gatatcaaat
      421 ttttttctga gaaacgtgat aggcacagcc tatctaccca ctttttttag aaagtgggta
      481 cacgtttcct tatgctcttt aaaattcggg ggggagtgct gcacgtgtaa tcgataacca
      541 aatttaacag acgaaaaatt gttggaatat aaaaatatga cacccgaaag aaaacgatcg
      601 ttggaaaaat taatgcaact agtgaacgaa gacggcggaa tttcagacgc agcttttgac
      661 gcatggagcg aatttcatgg agaatataat taagtgggtt taaatggctc taaaatcgct
      721 tctaagagtt ttttagcttg gaccaatgaa atacaccaaa aactaaataa aacgtcttaa
      781 atcgaaaaat acgagctctc agagcgactt tatttgtaaa tgttgcatat aagaaataat
      841 tacggatttt agacttttgt aggatatttt tgaaataaat atttatttct gttataattt
      901 gattgcaaat aagtgaacac gaaacacacg aaatccccag agaggtcgga ttctctgggg
      961 ctttttttgt gggctgagtc gccttaatta agttttactt cttgtttcgg ttattcaacc
     1021 agtaactgaa caaagcgctt aaaagcccaa caagaaggga gcaaataaag ttagtgaaca
     1081 cgaaacacac ctccttccag tcgcaagact gtcggatagg cgacaatcaa ttatacaata
     1141 aaaaagcaaa aataagccta agttttttat tgccttgggg ttgttagggc ttgccctgac
     1201 cgcctgtaag gcgctttaga acgaaggaca tagtttttat cgaatcttgt cttagaacgg
     1261 cttagacgag ctctcaggac gccacaaagg agtccctaat gaattatttt tggcgaagga
     1321 gcctaaaggc gactgctgca agtaaaatgt gagagcgtag cgatcactcc ttcttacagc
     1381 gaccataggg agctagattg gcagtttaat atcatgcgcg aacctgttta ttttaaaaag
     1441 tgggtgattt tttggcggga aattttgctc gcgcgcgcgc gtttatttta tttatttttt
     1501 aaagattttt tagaaccctt tttagggggc agttgagcct tactctccca acggatacag
     1561 aagggtacta acaacttttt atctgagtac taacaacttt ttatctgagt actaacaact
     1621 ttttatctga gtactaacaa ctttttatct gagtactaac aactttttat ctgagtacta
     1681 atatcctttt gtataataac aaccaaccta tttacgagtt ggttgttaaa ggttattatt
     1741 tatttgtaag gggggaagaa aggaatggat gaaaaaacgg aattaacagt caaatatcaa
     1801 aatgaattga atttagtacc gctgaaaaat ttcaatgcta agcagatgga tttatttttt
     1861 gcattatgcg ctcgtatgaa agatcgtggt ttgaaaaaag ttagattttc tttcgaggaa
     1921 ttaaaagagt taagtgatta taagatgact gctacaaaag cctttgtcgg tgatttagaa
     1981 aagctctata aagatatgct taatcttact taccgtactg aagatgagga ttctattcgc
     2041 tactttgttt tgtttactgg ttttgaaatc aataaaacaa aaaagtttgt tgaagtcagt
     2101 gttaatccgg acctagaata tattattaat ggtttaacag ctgaatttag tagatttgaa
     2161 ttatctgctt tcacgtcgtt gcgttctacg tatgcaaaaa ctctattccg actgcttatg
     2221 cagtaccgtt ctactggtta ttatgtagtt cgtattgaag agtttaggag cttaatggat
     2281 atcccagaat actatcaaat gggtaacatt gatcaaaaag tattaaatcc tgcattacga
     2341 gaactaaaaa aatacttcaa tgatcttaaa gtcacaaaaa taaaagcaaa aaagggcaat
     2401 aaaatcgcca aattagaatt tactttttca agcctaacgt cagatttgcc aa
//
LOCUS       DAZUKC010000168         2418 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000168 DAZUKC010000000
VERSION     DAZUKC010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.175"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..1149
                     /locus_tag="ACG80T_002765"
                     /pseudo
     CDS             5..1149
                     /locus_tag="ACG80T_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            1320..1778
                     /locus_tag="ACG80T_002766"
     CDS             1320..1778
                     /locus_tag="ACG80T_002766"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFS4842366.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1937..2407
                     /locus_tag="ACG80T_002767"
     CDS             1937..2407
                     /locus_tag="ACG80T_002767"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFS4842367.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          810 a          454 c          455 g          699 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgtcagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccaa aagatctagc cgatcagatc
      661 ggattttacc aaaatgctat cggagccgta cttggtcccc aagatagctg gttacttcag
      721 cgcggaatca agacactaag tgttcgtatg gaggaacatc aaaaaaatgc ttttgtggta
      781 gctgattttc tattttctca tccagcagta gaaaaggtct actatccagg attacccgat
      841 catgaactcc atggagttgc taagcagcag atgagcggct ttagcggaat gatttctttt
      901 actttaaaaa atgaagaatc tgctattcca tttgttgaat cactccaact atttacttta
      961 gcagaaagtt taggtggcgt agaaagttta gtggaaattc cttctgtcat gactcatgcg
     1021 tctattccaa aagaaaaacg agaagaggct ggcataaagg acggcttgat ccgtctttct
     1081 gtaggaattg agtatggtca ggatctcata aacgatttag ctcaggcatt tgacagaatc
     1141 aagaactaga agtcccaata aacgacttag cagaaaaatc taaaaaatat ttcagattgc
     1201 tacaccttgg aaacgataag tgttagaatc aagtagaaag acgtttttgt aaatttcact
     1261 aaataattct ttagtgaaaa gcatcaaaac gaatgaacaa acagatagga gtcttacaaa
     1321 tgagaatgaa tgtcgaaagt tttaatcttg accatacaaa agtcaaagca ccttatgtac
     1381 gtgtagctga tcgaaaaacg ggtgcgcatg gcgatgtcat cattaaatac gacgtccgct
     1441 ttaaacaacc aaataaagaa cacatggaca tgccaagtct acattcactt gagcacttga
     1501 ctgccgaact aatccgcaat cacgcagatt acatcgtaga ctggtctcca atgggatgcc
     1561 aaacaggctt ttacttgact gtattgaatc atgaaagcta cgaagacatt ttagatgttc
     1621 ttgaagcaac aatgaaagat gttcttgaag ctactgaagt acctgcaagc aatgaaacac
     1681 aatgtggctg ggctgccagc catacactag aaggtgcaca agctttggct cgtgaatttt
     1741 tagcaaaacg cgatgaatgg tctgaagtag aagcatagtt tctaaaagaa cgtaaagatg
     1801 agaagcaata tagctatctt atcttcgtat tttattaaat atttattgtc tgaaaagagt
     1861 tctaaacggg aaaacaaata gctagttttc agtttagcgc tctttttatt atataaaata
     1921 gaaaggagaa aactttatgt ctatttataa ttttgaaact actttagaag acggcactgt
     1981 ctattcattg aaaaaatatg caggcaaacc gataatcatt gttaacactg caacaaaatg
     2041 cggtctagca cctcaattta aacaattaga agaactttac caagcttatc agacacaagg
     2101 tctggttatc ttaggctttc cttccaatca atttaaacaa gagctagatt cagcgactga
     2161 agcagcagaa gcttgtaaaa ccacttatgg cgttaccttc cctatgcatc aattgattga
     2221 cgtaaatgga gaaaatacag atcctctttt tacttatctt aaaaatgaag ctcctggtac
     2281 tgtcactaaa gcaataaaat ggaattttac taaatttttg attgatagaa atggcaaagt
     2341 aatcgaacga tttgcaccac aaacttctcc attgaaaatg accgctgcaa ttgaatccgt
     2401 tctttaactt aataaaaa
//
LOCUS       DAZUKC010000169         2408 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000169 DAZUKC010000000
VERSION     DAZUKC010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.176"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(102..709)
                     /locus_tag="ACG80T_002768"
                     /pseudo
     CDS             complement(102..709)
                     /locus_tag="ACG80T_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            1232..1906
                     /locus_tag="ACG80T_002769"
                     /pseudo
     CDS             1232..1906
                     /locus_tag="ACG80T_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            1899..2060
                     /locus_tag="ACG80T_002770"
     CDS             1899..2060
                     /locus_tag="ACG80T_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842368.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
BASE COUNT          861 a          367 c          401 g          779 t
ORIGIN      
        1 ctatgaatat aaatagagtg tatcttcttt gataaatgtc agaaaaagtg tgacaaataa
       61 ccatcagtaa aattacttgt catactattg ataaggagta tttaattgaa gagaatgatt
      121 aggagaaagg atctccgtac tttttatagt aatcatagaa tccaagtttg tatagacgaa
      181 tctttttttc tcttatttct tctgtttcaa aatggactgc ttgaagaatc aagtctgtga
      241 attctatagg agctgttcca ttcgcagtaa tcaaattacg atctttcaca gcttgttgtt
      301 ctataaaatc ttctttattc tgataatttt ccgattctaa ccaaggaaat tgcgcatttc
      361 cggtgtgttt gtaaccttgc aataatccgc tctgagctaa ataatcaact gccccacaaa
      421 tagcagctac tggtacgtct gtttttaaca atttatcgat tatttgatat aaatgatgat
      481 tttttatgtc ccatgaattg ccaccgatca taataaaaag gtcaactttt tcaggtattt
      541 gttcgattaa gtaatcgatt tttgttttta atcctccaat ggaagtgacc tctgattgga
      601 tagaagcagt tttgttttgc catccctctt tttggtttag tgtgctagta atataagcac
      661 cttcccaatc ggcatattcc tctaatagaa acgtaatcgc agttttcatt ttcaagctct
      721 cctttactat ctatgacaag gacaacagtt ttaacataca gacattcatt aaatttggat
      781 agcgacagcg aaaacaacaa ttcttaagtt ttagacagag tatacagaca gtcaacttaa
      841 ttaaatacga actaaataaa aatgacaggt aatcattttt gtttaccagc attataacat
      901 tttgcataca tggatgacaa aagaaccaat tcccacttgc ttgagtacaa tcttaaaaga
      961 aactggaagg caaaaagtaa ttatagaaac gaaaaataaa tcatatcggg cactcagaca
     1021 tgaaaacaac cctagaaata tataactagt ccaatgaaaa aaaagcgaat gccgtagaaa
     1081 acaaggattc tgcgtacatt catatctcat tttgcgagca tatctgctat tactgtgact
     1141 aaatgttaat atggttaaaa tgaccgttat attttttata taaatatcgt ataattagta
     1201 tgaatataga agaaggaaag aaggtgcaat cttggaacct aaagacaagt atttagatta
     1261 cgctagggtc aataaagata aatttattga aacaatcatc aagggaaaaa tccctgatga
     1321 tgaaaaagat gcggtattca tggctggaag cccaggagca ggtaaaacag aggttgcact
     1381 aggtttagct taaaattatg acaaccatgt tgtgattgat gcggactatt ttagagcaca
     1441 atttccagaa tacaatggaa agaactcaag tgtttttcag aaagcttctt cttggttagt
     1501 tgaacaatca ttaaaatatg tactggaaca tggctattct ttcatactag aggatacttt
     1561 tgctatcctt agtgcagaga aaaatattat tcgagccctt aaaaataact ttcgcgttac
     1621 gattttctat gtttaccaag atcccaaagt tgcctgggat ttcactcgaa aacgtgaact
     1681 agctgaagga cgtcatgtgc caaaagaaat gtttatcaat gcgtttttta agtccagaga
     1741 gaatattgaa aaagtcaaaa atagacatcc tgaagtagtc cttcacataa tgattaagga
     1801 ttatcaaaac gacatttctg aagtgcatta tgcagcagat aatattcaat tagtactacc
     1861 aatacaatat acttcaaaag agttggagga ggaattacat gactgagaaa gaacaagtac
     1921 aacaaatcgt gaagaagtat aataaaagta tcgctgattt atcagaaaac gcctcagcga
     1981 aagaatttaa aacagtaatg aattacgtag cagatgaagc aaatcgtaag cagcgaaaac
     2041 tagttgggtt ggataaataa attttttaaa ccatatcctt gtaaagttga gttttttatt
     2101 ttaatgaatt aagactatta aatagataat tattgtaact taaaaaacac agctaaaaat
     2161 tagttgtgtt ttttattagg tttacttttc tggtataaaa ggaactagct actttttcaa
     2221 aatttctaaa tcattcaata tgtagcgctt actaaattat tcgccataaa attttataat
     2281 tggatcactc attgttttac ctaagtcata tttaagtaat aaccggtgtt tttacataag
     2341 aagacttcgg gtatttttta gtttgattag ctctgatata cctataaaga atatgctaag
     2401 gtaagaat
//
LOCUS       DAZUKC010000170         2406 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000170 DAZUKC010000000
VERSION     DAZUKC010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.177"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..433)
                     /locus_tag="ACG80T_002771"
     CDS             complement(<1..433)
                     /locus_tag="ACG80T_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFS4842369.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDV"
     gene            complement(411..725)
                     /locus_tag="ACG80T_002772"
     CDS             complement(411..725)
                     /locus_tag="ACG80T_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFS4842370.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            complement(828..1109)
                     /locus_tag="ACG80T_002773"
     CDS             complement(828..1109)
                     /locus_tag="ACG80T_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842371.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            complement(1114..1458)
                     /locus_tag="ACG80T_002774"
     CDS             complement(1114..1458)
                     /locus_tag="ACG80T_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFS4842372.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            complement(1484..1651)
                     /locus_tag="ACG80T_002775"
     CDS             complement(1484..1651)
                     /locus_tag="ACG80T_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFS4842373.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
BASE COUNT          688 a          518 c          344 g          856 t
ORIGIN      
        1 atacatcatc aaaactagtc tttgcttggc tttcattatt ggcatagata tcaatatcat
       61 aatttttaat accgtgttta gcggttagta gaaagaagtt gttccaagaa gcaaaaccag
      121 ttttaccatt accacgtccc attaatgaaa gatatctatt gaacactagt gttttatctt
      181 ttttccatcg aacaccataa ataaaacatt gtagaaattt ttcccacgga attaattcga
      241 atggaaagta ttgtgctggt atattgattg aatcctctac catctgctta tcgaagtaaa
      301 tatcttctct agtaaagact ctttcttcta gataattttt tagcaataat tgctctttgc
      361 ataccttgat agtgccttct tctatagctt tgaaccaatt ttcaatatgc ttataactca
      421 ggaattgatt catttgcttc acctaccaat tcaggagtaa tggcaagttt atccaacatc
      481 aatcccattt gtttgttgac agaaacaagc aacgctactg attcattctt tttaccattc
      541 tccagtctaa tgccgttctc ggatatatct tcttccagtg atatcgccgt ttcccataaa
      601 ctgatataac gatcaacatt atctaagaat ggctcaatat ttgttttctg actttccaat
      661 tggcttatta aagagcggcg taatttttct ctgtagcgat tttgagacaa ttcgttttta
      721 aacattttag ccctcctttc atgataaagt tcgaaaaaat ctcttttcct gacagccccc
      781 tccgtttcat cacccccaaa aaatttgcga tttattttaa gggggggtta tctcaccatc
      841 ggaatgaaag cttcagcgaa gtcaatgtaa taattaatct cttcaatact atatccaaaa
      901 acatttttta ttctttcgac gttattatct ttattcaacg cttctcttac ttgattcact
      961 ttgtatttac tacaacagtt atctgataac agatccctaa tacctacata gcgaacgtat
     1021 atcaaacgtt taattaatcc ctgagtataa gatgaatact cttcaatctt ttctgtatca
     1081 tactctctgc cattatcatt gatgatcatg cacttaccac ctttcacttg catcgaagtt
     1141 agcaaagctt tctatcttct tctcttgttt atctaatgct gtaagatatc tgccatgaac
     1201 ttcattatga tgttcaacac ataaacaaat aagattatct aaatctaaag ctaagtcagg
     1261 tctatccttg acttccttta tatgatgaac gttctctact ctatgatact tacctagtct
     1321 tctacactct tggcattcat agtgatctcg tttcatcgct ttctctctaa gcctgcgcca
     1381 tttaggagac tgatagaact taaccaaacg atcttctctt gtcaactgta atagccatct
     1441 atagaattcc tcggtcatgt tccgtctcct ttcgcaatct tatttaatgc ttagctattc
     1501 ttttgccata caatggaata acttcattgt tttcctttcg tttatatgta tcgctcttta
     1561 ttggtcttct atactcatat actttttcgc cattaccttt atgaacagtg attacttcat
     1621 acttctgttc taagtattgt ggtctataca ttgttgtttc ctcctttgtg caaaaataaa
     1681 aagaccactc aaagagtgat ctaatatgta atagtaactt gcacgatgca caaaacgcgt
     1741 acgaaattgc gcacccctat atttttaaac cgccgatgcc tcggttgctt aaagtcgctg
     1801 gagtgggatt gcaccactca cgagaactta ccaggctctc acgaggctac tcgccattta
     1861 ccgctgcgtc ttctacttcc gccacagtga ccgaagcttg gtggtgtaca cagatagcac
     1921 acttactaca ttgccgtacg tagcaccgta tagcttctta acgatctttt ttcggtagtc
     1981 gtaaccgtca taggcattta atgtcgctgg caaggaatcg aaccttgcat ggttgccgaa
     2041 gcattgacct agcacacatg cttagcgtct accctttccg ccacagtgac actataaaat
     2101 tattcttggc tgctactatt ttttattttg cccattttta aatccaatca tatagacatt
     2161 aagacagagc gcaaaaattg aaattattaa tggaatcatt tctcttcacc cacctttagt
     2221 tatcgtgtga ataattaaaa aacaatagac agcaacaaaa taacattgct ttgataattt
     2281 ggtataaacc actataaatt tcttttcttg caattatttt taatatatgc tagattatca
     2341 accgatatag tcactgcctg tactagcgga aactagtgca ggttttttgt tctatttact
     2401 caaaag
//
LOCUS       DAZUKC010000171         2385 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000171 DAZUKC010000000
VERSION     DAZUKC010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.178"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            306..560
                     /locus_tag="ACG80T_002776"
     CDS             306..560
                     /locus_tag="ACG80T_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS4842374.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            561..878
                     /locus_tag="ACG80T_002777"
     CDS             561..878
                     /locus_tag="ACG80T_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS4842375.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1437..2372
                     /locus_tag="ACG80T_002778"
     CDS             1437..2372
                     /locus_tag="ACG80T_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842376.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          853 a          338 c          453 g          741 t
ORIGIN      
        1 aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc ataccgaacg
       61 gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt aaatggatgt
      121 taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta tatgttttca
      181 gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct taagaaacac
      241 cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa aaggagtgtg
      301 ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga gtgatccttc
      361 caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa ggcaacttat
      421 tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt gaagaaagtt
      481 tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa gctttcggaa
      541 aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa agttttcaga
      601 ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct ccagccagca
      661 taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat tttccaacta
      721 tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg atcgtgatcg
      781 gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata cttgtaggac
      841 gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga aaaagtcgct
      901 catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt cggttactcg
      961 aagattgata tataagactt agtacctttt tattttgctc catgttatgg ccgaaggtct
     1021 aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg gggatcgctt
     1081 ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc tctattccca
     1141 aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc aaaattgacg
     1201 taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt taaaagagga
     1261 agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa aatatacatc
     1321 cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa aggaatggga
     1381 taaaatatag gtacaataac tatttgatgc taaatataat taatggagga gagatagtga
     1441 acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt actactttca
     1501 aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa ggatacaagt
     1561 ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt ctttctgaga
     1621 gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt gatatatata
     1681 cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc agtgaatatc
     1741 tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta ctaccatatt
     1801 tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca ggtaatcaag
     1861 gaaatatgaa tattacaagt aacaaaacgt cacgtgtgct attggcgttg atgggttacg
     1921 gaattcttta tagagatgac caaaatggcc agattctaat aaatcgggct gggcgttcat
     1981 tattaatgaa atatagaaaa tttataccgg aagattttca aaacttacag ttagaaaaca
     2041 tgcgtttaga agatttattt agattagcag aagatgataa gtatagattt gaagttttga
     2101 aaattcaaaa gaatgaacta ttgaataagt tatatcctga gaatgcgata aaagcacatt
     2161 ttggtgatgc taatcatatg tatagttggt tcattcctaa aagatttaga gggaaaatca
     2221 atgctggaga tcaagtgaaa gtactcaatc gaaatagtac agatgtcgta atgataacag
     2281 atatctatgt agaagatgaa gatataatca aatatatgaa gcctgtgata aaacttgtta
     2341 ataaaaataa aggaacccgt ggtgctagtt aattttttcc tagag
//
LOCUS       DAZUKC010000172         2356 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000172 DAZUKC010000000
VERSION     DAZUKC010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.179"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            30..>2356
                     /gene="esp"
                     /locus_tag="ACG80T_002779"
     CDS             30..>2356
                     /gene="esp"
                     /locus_tag="ACG80T_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFS4842377.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT          856 a          370 c          482 g          648 t
ORIGIN      
        1 agtaattatt aagggagttg atttgaataa tggttagcaa gaataataag agagtatttc
       61 tagaaaaaac aaagaaacga gtattgaagt acagtatcaa aaaattaagt gttggggttg
      121 catctgtttt gattggtgtt ggtcttgtct tgggaacaac tgaactagtt aaagcacaag
      181 atgaaatttc tccaagcagc ccacttgaaa cagcgataag ctccgtacaa gtaggtgaca
      241 aagtagcttc ggggaatact tttcaggaga atcctggtta tacgaaaaat tataactttt
      301 cggatttaca atttagtcct caagaactaa ctggagacac actaaaaggc aatacaattg
      361 gatttgaagt ttacggaaaa cataatattg cagcatcaac taaaaactgg gaaatccgtc
      421 ttcaattaga tgaacgatta gctaaatatg tagaaaaaat tcaggtcgat ccaaagaaag
      481 gaattggatc ttctagacgg acctttgtaa gaattaatga ttcgctgggt agacctacaa
      541 atatttggaa ggtcaattat attcgtgcaa gtgatggatt atttgctggg gcagaaacaa
      601 ccgatacgca aaccgctcct aatggggtaa ttacgtttga aaagagccta gatgaaattt
      661 ttaaagagat aggcatagac aatctcaaga ccgatcgttt aatgtatcga atctacttgg
      721 taagtcatca agacgatgat aaaattgtac cgggtattga tagtacgggc tactttttaa
      781 cagattcaga tgatttctat aatagcttag atgtatctga aaataatcct gaccagttta
      841 aacatggatc agtaagtgca aaatatgaga aacctaatac tcaaacaaaa gatggttcag
      901 gatccacagg tgctaatgga gctataatct tagatcataa gttaacgaaa aattataatt
      961 tctcatattc atcttccgca aagggcacgc cttggtatgc taattacaaa attgatgaac
     1021 gattagttcc atatgtagcg ggtatacaga tgcacatggt tcaggctgac aaagtaacat
     1081 atgatgtttc gtttgaatcc ggaacaaaag tagctgattt agcaatcgag cgacgggaag
     1141 gtcatgaaaa ttatggcata ggttcaataa ctaataatga tttaactaaa cttatcgatt
     1201 ttgctaatgc aagtccacgt ccagtcgtta ttcgatatgt tttacaattg actaagccat
     1261 tagatgaaat cttagaagat atgaaagcaa cagcacaagt tgaagaaaat aaaccatttg
     1321 gtgaagattt catctttgat tcttggttgt cggatacgaa taaaaaatta atccagaaca
     1381 cttatggaac aggttattat tatttgcaag atattgatgg tgatggaaac cctgacgata
     1441 aagaagagag cggagacacg aatccatata tcgggaaacc tgaattagaa gaagtatatg
     1501 atgttgacac aacagttaag gggaaagtat tcatccacga gttagcggga acaggtcaca
     1561 aagcccaact tgttgataaa gaaggtactg tattagcaga aaaaactatc gctccaaatg
     1621 aaaaagatgg ggctccaatt tcagatactg tagaatttga atttacgggt gtagattcaa
     1681 gtaaattaat cgcgaaagat gaattaaaaa tccaaatcgt ttctccaggt tttgataaac
     1741 cagaagaagg ttcaaccgtt attaaggaat caccaaaagc ggttgataaa caaaccgtgg
     1801 tagttggatt taaaccagat gctaaagaat caattcggaa taataaaaac ttacctgaag
     1861 atgcagagta ttcatggaaa acagagcctg atacttctaa cgttactgat agtacgaaag
     1921 gtattgtaac tgttaagatc ggaaatcgaa ctttcgacgt ggatgtagag tttgctgtaa
     1981 aagcttctca agctatggaa aatgatgcaa catacgtacc tataacaaca actccagaaa
     2041 cgacagttca aagtggaaaa cctacatttg ataaaccaga tgttccttta gctaaagatg
     2101 ccttttcaat ttcagatgtc tataataagt acttcggtaa tgcaagtgtt gatgcaaaca
     2161 ctggtattgt tacattcact ccagctaaag gtgtaggaga atcggagccg attactggaa
     2221 caattcctat taaaattgtt taccaagatg gttctgtagg cacgaccgat ttagcagtaa
     2281 ctgtaagtaa agatatttat gaaaatccag gagaaaacat tcctgcaggc taccacaaag
     2341 taaccttcac cgcagg
//
LOCUS       DAZUKC010000173         2335 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000173 DAZUKC010000000
VERSION     DAZUKC010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.180"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(51..1598)
                     /locus_tag="ACG80T_002780"
     CDS             complement(51..1598)
                     /locus_tag="ACG80T_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFS4842378.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="ACG80T_002781"
     CDS             complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="ACG80T_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFS4842379.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(2043..2237)
                     /locus_tag="ACG80T_002782"
     CDS             complement(2043..2237)
                     /locus_tag="ACG80T_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842380.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          653 a          483 c          420 g          779 t
ORIGIN      
        1 tacgtcaaat aacccgacct atagaataga atcgggaatg tttggcgttt ttagttacga
       61 gaaaaatgtt gttgtacatc cttgttccaa ggcaacagtt gatctaaagt ttctgctgaa
      121 agtacgggaa gatttggtat cttatcaaat agaaactcta gatactttac aggattcaat
      181 ccgttaagtt ctgctgtttt atatacactc agaatgattc ctgaagttcg tgctccttca
      241 agacttgtgg aatgcaacca atttttccgt cctatcacca gctctttgat atttcgctcc
      301 gcacgattat ttgtgagttc taaacgacca tcttcaaaaa taggttcaaa accacttcgt
      361 agtttacacg catattgaac ggctgcatcc aacttggatt ttgcaacagg atctatcgtt
      421 gccatccact cataaaaagc aataaataat ggacgcagtt cttgttgccg acagcttaat
      481 cgcttctcag gagaaaagtc tttccatttc ttttctagga caaaccattt atccaattgt
      541 ttgactgctt gagctgcggg aatatccgtg tttttctttc cttttggaat tgcttcgaaa
      601 aatttccgcc gaacatgcgc gaaacaatag acaagtcgta cactttccac ggcattatac
      661 ccgctatagc cgtcacagtg taaataccct gtaaactcct tgagaaaagt tttcggaaca
      721 tctccagcac gactttcgtc gtggtgataa taaacgatgg gatgctgact gtcttttcct
      781 gtacaaaact gccagtaata ggtatctgtt ttggcacttt caatcacacg ataacttgtt
      841 tcatccccgt gtagaatctc ttcttgatta agatagactc ttaatcgttc tcctaaaggg
      901 tctaacgcgt tttgcactgc tttaatatgc caattggtga tattatctct cgaaatatcc
      961 aacccatgtg tttgtttcca atagttctct tgccgatagg caggaacttt ctgaccaaat
     1021 ttcatccgga tcgtttccgc cactaaacta ttggaaccta agctattaga aatcaaaggt
     1081 ttaggaacga tcgctttttt aaaagaagaa agtccttctt tttcgcagct tttacaacaa
     1141 taggtatgtt gataatgggt gagacaatcc aacatagcct gatggaaacg aatttcttct
     1201 cgtacttttg tccgtccgat atctttcatc tgtgaaccgc aatgttcaca aaattgttct
     1261 tccaaaggaa ggagatgttc tttctctgtt ataggtaagt gtttgatttt gtctgctttc
     1321 gttcctacaa ctcgttgatg tccttttact ggaataatgg tggcagctgc ttctggaaca
     1381 cttacagaaa tttcttcatc actaaaaaga ttcaattgcc cattcgtttc ctcaaagaga
     1441 gtctctttag agcgtccaaa taatttgttg gttaaataat tgaccttttc attcaatagt
     1501 tggatttcag ttgtaagatt ctgaatggtt tgttgttgtg cttgattggt tttatgggct
     1561 tcttgaagca actgaagcaa cgtatctgta gtttccaaaa tttttcacca ccttgaatcg
     1621 aactgatttt gttacagaat accacaagaa acgcacaaga aataggggtt caacacattt
     1681 tttgtgttaa ccccccagct taaaaaactc ctttttttcc tggcaaaata gctcgaggtt
     1741 gatcgatact taatccttct aacaaccatc gaacctgttg gttcgttaag gttcgaactt
     1801 cgtctttcgt ccgaggccat tttaacttgc cgttttctat ccgcttataa cagagaagaa
     1861 aaccatcacc gtcccaatag agaagtttga aacggtcttg acggttcccg caaaataaaa
     1921 aaacggcatc ttcgtaaagc tcgagttgat actcttgttg aataagactg gcaagaccat
     1981 caattccttt tcgcaaatct gtttttccgc acacaatata aatatgtttg gtttgaagta
     2041 aatcataaag cattggacaa cagttctttc aaaatagctt gaagaatata attgttaata
     2101 ccgttataga tcaaaacttc agaaccattc tctcttttga tgcgcatcgc taacttggca
     2161 gccgcgggtt tacctttttc tggtgcactc ttttcgtttc ctaattggac aggaacaatc
     2221 tcatttaatt tatacataga agacctccag acttcttgat agctcaagta tatcttgagg
     2281 tgcatttgtt agtaaggtac tgttctatgg ggcgcttacg ttaaaaatgt tttgc
//
LOCUS       DAZUKC010000174         2261 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000174 DAZUKC010000000
VERSION     DAZUKC010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.181"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(365..1657)
                     /locus_tag="ACG80T_002783"
     CDS             complement(365..1657)
                     /locus_tag="ACG80T_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS4842381.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(1931..2191)
                     /locus_tag="ACG80T_002784"
     CDS             complement(1931..2191)
                     /locus_tag="ACG80T_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842382.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          746 a          430 c          344 g          741 t
ORIGIN      
        1 tatgggctac tctgtgatac ttattagcca ttaaactatt ttacccacat agcatatatt
       61 ttgaaatcgt tcattccgta aattcaacta aatatgccta aaaaaataaa aaatctaaga
      121 tagaataaga tttattcagt cttagatgga tttatagcaa ctttttcgta acaactttaa
      181 aaaatacgaa aagaaagagc tgttttactc tgactacgtt gctaaattgt cgtttaatca
      241 ttcatttaaa ccaaatcttt ttttatagaa ttcgttttaa tcctcctgaa tccaaaaact
      301 atgaaaaccg taattaaata gttaagagtt tttaaccacc ataattctct cggctatttt
      361 gaaatcagat aaattttgat cggacacata gttgaataaa cctaccgttg ttacggaagg
      421 taaatcgcat acttttcatt ctagaggaga aggtctcgtt gatcattaac tgattaagca
      481 ccatagccca gttttgaatt cgaccacctg accacttcgc gtgtaattct ttcgtgcgta
      541 aataaagtag ttttaggagt gcattctcat tagagaacgc tccttttttt gtgacttttc
      601 tgaagctgga gtggacactt tcaaccgcat tggtagtgta cataattttt cgaatggcac
      661 taccataatc aaatagttgt tcaacatgtg caaagttccg tttccagaca tctacagcac
      721 cagaataatg agaccaccga ttttgaaaac tgtcaaaagc agcatgtgca gcgtttagag
      781 aagaagcacc gtagaacttt ttcatatctc ggcagacttc cttatagtcc ttacttggaa
      841 tatagcgcaa tgcatttcga acaagatgaa caatacagcg ctgaacaatt accgatggaa
      901 agatcgcttt tgcgccttct tcgaggccag aaacaccgtc catcgaaatg aaaaagacat
      961 cttcgacacc acgtgctttc agttcatcaa atacttgcat ccagcgattt ttagattctg
     1021 tttgatttaa ccataatcct aaaatctcct tatttccttt gagatcatag ccaagaatgg
     1081 tgtatacagc atattctttg gcttcataat tttctcgtaa agtaacatac atacaatcaa
     1141 cgaatagaaa ggcataacac tttgctaggg ggcgggcttg ccattcttcc aattcaggaa
     1201 ggacagcgtc agtgatatct gaaatcattt catgggaaat atcaaagcca taaatagctt
     1261 cgacggttgc ggcaatatct cgttgactca ttcctcgtgc atacatggaa agaaccttcc
     1321 cttcgatgtc ggagacatct cgttttctct taggaattaa ctctggttca aaggaagctt
     1381 cccggtctct aggaacatca atagctactt caccaaaact ggttttaagc gttttagttc
     1441 catagccatt tcgacggtta tcgtgttcct taggctcttt agaatgggca tcataaccta
     1501 aatgattatt caattctcct tgaagcattt tttcaaaaag gggcccaaac acatctttca
     1561 aagcatcttg catgtcatcg acagattcag gttgataggc attcagaatg gattcagcta
     1621 acttttcggc atcaggattt cttttctttc tagccatcgt gtaatcacct cttgatctta
     1681 ttgtagaaaa agaaaaaccg cgaagcaacc acctgcctag gattaatggt tacttacacg
     1741 gtttatatta cactctcatc attaaaaaag tatctttcga tattactaaa caatgaaatt
     1801 atctatttga aatttaaatc tgataatata ttgagtgttc caatgagggc tttgatccta
     1861 ttatccatat ctttttcagt tttaatattg atacacatta taagaaaaag aaacttttgg
     1921 ttaatttatt tcatcccagt gcaaaatata aaaaagtaaa atagaccata ggcaatagat
     1981 taacaattaa tataatacaa gcaaatattc tattcattgt atttctgctt tcaaaaagtt
     2041 ctaatattga aagtataatt ccacaaatgc ctataataag tatgggaata agattagaaa
     2101 ataacacccc gagccaatct gtgaaaatta ctatatttac taatatccct attaacatac
     2161 ctatcatatt tattaaatca atttttttca ttagatattt ctcctttatt aaatctgaag
     2221 tacttatatt aaaataattc taaactattt acctcaaaaa g
//
LOCUS       DAZUKC010000175         2255 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000175 DAZUKC010000000
VERSION     DAZUKC010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.182"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(144..>2255)
                     /locus_tag="ACG80T_002785"
     CDS             complement(144..>2255)
                     /locus_tag="ACG80T_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFS4842383.1"
                     /translation="LEKIRKGAQEPKNKTFRRSHVPFRLNFLFFIIFTLFVALIARLG
                     YLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKANQAITYTRGTQVTA
                     KDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKRLSAKEKQLGNSEQY
                     SAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGVTDQELAVVAERASE
                     LPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLAKGYSRNDRVGQSYL
                     EKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSMNAEFQSKVEEILKR
                     NYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEITENALGTITSAFDP
                     GSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRSGQVALDAVKASELS
                     SNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFGLGTTTGIDLPNESP
                     GISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQYASTVANGGKRVQP
                     HLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGFHQVVHGTDPYTTAK
                     PLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEIAISVVLPNLLDEQD
                     HINLTIAKEIMDAYYDMFMAK"
BASE COUNT          574 a          505 c          411 g          765 t
ORIGIN      
        1 gtcacaacct tttttgccga gtaaacggtg ttcaaaagcc agtttctcgg tatgaagtca
       61 aatttaagca aaaatgtgag aagctatttt cacaaaattt ttcttcatac tcgaaactaa
      121 gcggcttttt cacgacctct tttttatttt gccatgaaca tatcataata cgcgtccatt
      181 atttctttag caatcgtcaa gtttatgtgg tcttgttcgt ctaataagtt cggcaagaca
      241 acactgatgg caatttccgg attgtctgtt ggtccgtatg ccacgatatt actattgact
      301 gtcgcaatat ctggatgacc ttccgcaacc gtttctgcag tccctgtttt tgcggaaagg
      361 tccattttcg cactggctaa tggtttggct gttgtgtaag gatcggtacc atggacaact
      421 tggtggaatc cttcacggag gatcgccata ttttctgcag aaatattgac cgtattttct
      481 actgttgtgc caatttcttt cttcacttca cccaagtttc cattttcgtc attgccatag
      541 atcgctttga ctaggtgcgg ctgtactcgt ttcccgccat tagcgactgt tgaagcatat
      601 tgagctaatt ggattggtgt atatgtgtca aactgtccaa atgccaaatc ggtaaagtta
      661 cctggagcat accattcttt gccattatcc gcattgaact tcttcatata atcaacagaa
      721 cgtgagatcc caggagattc attcggcaaa tcgatcccag tcgttgtacc aagaccaaac
      781 tctttgaaag agtctctcaa ttgctgataa gctttagctt cttcatctaa actaggtagt
      841 cccattccag gtgtatagtc tagtcctaac atctttaacg ccactttgat catatacgta
      901 ttagaagaca attccgatgc tttgactgca tcaagagcaa cttgtccgct tcgattaaag
      961 acagagcttt tttctgaagc accttgcaga cgaatcggct catcaatcaa aacttgattt
     1021 ccgctgatcg cgttacttgc ccagcctgct gtcaaagtac cagccttgac tacagaccct
     1081 ggatcaaagg cgctagtaat cgtgcctaaa gcattctcgg tgatttcttt ggaaccttgt
     1141 tcatgactga aaccagtcat tgctagcact tctcctgtct gaggattcat agccactgca
     1201 taagcaccag gcgaatactg tgctttgccg ttgttgatca atgtttgata attccttttc
     1261 aaaatttctt ctacttttga ttggaattct gcattcatag ataacattaa attagaacct
     1321 ttttccccag agaagatttc tttttgattc gaaacatttc cttcattatc aagggaaact
     1381 tcatattggg ttttggttcc ttgcaggaca tcttcatatt gtttttccaa ataactttgt
     1441 cctacacggt cattccgaga ataacctttt gctaggtatt tctctgcttc atctgctgga
     1501 agtccttgct tttcagtcgt cacactgcca agaatacttt tcagtgaact tgctgcatta
     1561 tattctcgtg tccagtctgt gccagtcgaa actcctggaa gttcagaagc gcgttctgcc
     1621 acaacagcta actcttgatc agtaacacca gagtttttga tatagacagt gtttaattca
     1681 tatgcactat tcatccgttt aaagatggta gcaattttta attgctcctc gtttaaatta
     1741 atctcatcag ctgtgacttt attgaccatc gcactatatt gttcgctgtt ccctaattgc
     1801 ttttcttttg ccgaaagtct ttttgtggct tctttcaaat gtttgggatc agccagccaa
     1861 aaatcttttt tatctctatc tgtcagattt tcatctaccg gaacatcgat cagcgagttc
     1921 agcttcgttg cgatttcaag caaatcttta gccgtaactt gtgttcctct cgtatatgtg
     1981 atggcctgat tcgctttatt cgtaactaaa gcttttcctg tggagtcgta gatcattccg
     2041 cgaggagtac tttcttggac cgtaatcgta gaagtagact tcactcgcgc ctccatctct
     2101 tcgccgttca cgatctgcaa ataacccaat ctagcaatca acgcaacaaa taacgtaaat
     2161 ataatgaaaa aaagaaaatt taagcgaaaa ggaacatggg accggcgaaa cgttttgttc
     2221 ttaggttctt gtgcaccttt tcttattttt tctaa
//
LOCUS       DAZUKC010000176         2198 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000176 DAZUKC010000000
VERSION     DAZUKC010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.183"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..942
                     /locus_tag="ACG80T_002786"
     CDS             <1..942
                     /locus_tag="ACG80T_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS4842384.1"
                     /translation="GCQIIETPIDRRGINPKTDFKLFFNYCNILKKIKPDMVIAYTIK
                     PNIYGGLACRLRKVSYALNITGLGTAFQKKGVLKSLIVFLYRLAAKKSKVIFFENFEN
                     MKTFQTLQIAKQSKYKLLNGAGVDVEHFSYLEYPKDEQVHFLFIGRIMKEKGVDELFE
                     AMKNLYSEEKNCVLDVLGYFEEDYGIKIEKYEKEGWLKYHGYQSDIRPFVERAHCFVL
                     PSWHEGMANTNLESAASGRPIITSDIPGCKEAVIESKSGYLCERKNSCSLYNSMNKFL
                     KLSYEQRINMGKMGRNYMIKKFNKKKVVDETIKSLFS"
     gene            949..1344
                     /locus_tag="ACG80T_002787"
     CDS             949..1344
                     /locus_tag="ACG80T_002787"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4842385.1"
                     /translation="MFCKVCDRKEGFCGFRIDIKELAKVSDIFLFTTLQEGMPRSMME
                     AMASGLPCIASKIRGNVDLLVEGKGGYLVPAKDAELVAECLRELINNKSLRKYMGNEN
                     LSRIKEFEVSIVKKRIKEIYEEVIGESVK"
     gene            1341..>2198
                     /locus_tag="ACG80T_002788"
     CDS             1341..>2198
                     /locus_tag="ACG80T_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4842386.1"
                     /translation="MKNPMFSIIVPIYCVEKYLKDCLDSIRNQTFKNFEAILVDDGSP
                     DECPEICDYYSQIDGRLKVIHKKNGGIIDARKIGVTSAMGEYIVFLDGDDWLANTYLE
                     KYAEVITKYNPDIICSGYFASRSNKIRKRELSCSFGYYDKNKIINLIYPKLIEDEHGE
                     YFSPQLWAKAFRTTLYKEQQQVSGQVSVGEDHACTKPCIYHANSIYMMEKCLYYYRIN
                     ENSITKKPQPYYWEGPKAIGEHFEKQIDMNKYDFQDQVYRNVTHNLFNVAVSQFNKQD
                     KYLNIKKDII"
BASE COUNT          869 a          242 c          419 g          668 t
ORIGIN      
        1 ggatgtcaaa ttattgaaac tccaatagat agaagaggaa ttaatccaaa aactgacttt
       61 aaactatttt ttaactattg taatattttg aaaaaaataa agccagatat ggtcattgct
      121 tatactatca aaccaaatat atacggtgga ttggcatgtc ggttaagaaa ggtttcttat
      181 gcattaaata taaccggatt aggaacggca tttcaaaaaa agggggtttt gaaaagtctc
      241 attgtatttt tgtatagatt agcagctaaa aaatctaagg tcatattttt cgaaaatttt
      301 gaaaatatga aaacatttca gactttacaa attgctaaac aaagtaaata caagctatta
      361 aacggtgcag gagtagatgt agaacacttt agttatttag aatatcctaa agatgagcaa
      421 gttcattttt tatttattgg aagaattatg aaagagaaag gtgtagatga actttttgaa
      481 gcaatgaaaa acttatatag tgaagagaaa aattgtgtac ttgatgtcct tgggtatttt
      541 gaagaagatt acggcataaa aattgaaaaa tacgaaaaag agggatggtt aaaatatcat
      601 ggatatcaat ctgatattcg tccctttgta gaaagagcac attgctttgt tttgcctagt
      661 tggcacgaag gaatggcaaa tactaacctt gaatcagcag catcagggag accaattatt
      721 acaagtgata tcccagggtg taaagaagca gttattgaat cgaagtctgg ttatttatgt
      781 gaaagaaaaa atagttgtag tctctataat tcgatgaata aatttctaaa attatcatat
      841 gagcagcgca ttaacatggg aaaaatgggg agaaattata tgattaaaaa atttaataag
      901 aagaaagttg tcgatgaaac aattaaatca ttatttagtt aaaaagatat gttttgcaaa
      961 gtatgtgata gaaaagaggg attctgtggc ttcagaatag atatcaaaga attggcaaaa
     1021 gtttctgata tttttttatt tactacatta caagaaggaa tgccaagatc tatgatggaa
     1081 gctatggcaa gtggattgcc atgtatagca agtaaaatcc gaggtaatgt agacctttta
     1141 gtggaaggaa aaggtggata tcttgttcca gctaaagatg cagaattagt agcagagtgt
     1201 ttgagagaac taatcaataa taaatctctt agaaagtata tgggaaatga gaacctttcc
     1261 agaattaaag aattcgaggt tagtatcgta aaaaaaagaa tcaaagaaat atatgaagaa
     1321 gttataggtg agagtgtaaa atgaaaaatc caatgttttc aataatagta cctatctatt
     1381 gcgttgaaaa atatttgaaa gattgcttag attcaataag aaatcagaca tttaaaaatt
     1441 ttgaagcaat actagttgat gacggtagtc ctgacgaatg cccagaaata tgtgactatt
     1501 attcccaaat agatggacgt ttaaaagtaa ttcataagaa aaatggtgga ataatagatg
     1561 cacggaaaat aggagtcact agtgctatgg gagagtatat agtttttcta gatggtgatg
     1621 attggcttgc aaatacttat ttagaaaaat atgcggaagt aattactaag tacaatccag
     1681 atataatatg tagtgggtat tttgcatcac gttccaacaa aattagaaaa agagaattgt
     1741 cttgctcgtt tggatattat gacaaaaata aaattataaa tttgatttat cctaaattaa
     1801 ttgaggatga acatggagaa tattttagtc cacaattatg ggcaaaagct tttagaacta
     1861 ctctgtacaa ggagcaacaa caagtatctg ggcaagttag tgttggtgaa gatcatgcat
     1921 gtacaaaacc atgtatttac catgcaaatt cgatatatat gatggaaaaa tgtttatatt
     1981 attacagaat aaacgaaaat tctataacaa aaaaaccaca accatattat tgggaaggac
     2041 caaaggctat tggagagcat tttgaaaagc agattgatat gaataagtat gattttcaag
     2101 atcaagtata tagaaatgtt acacacaatt tatttaatgt tgctgtttca caatttaata
     2161 aacaggacaa atatttaaat ataaaaaaag atattata
//
LOCUS       DAZUKC010000177         2168 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000177 DAZUKC010000000
VERSION     DAZUKC010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.184"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            71..>2168
                     /gene="acm"
                     /locus_tag="ACG80T_002789"
     CDS             71..>2168
                     /gene="acm"
                     /locus_tag="ACG80T_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFS4842387.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFA"
BASE COUNT          854 a          342 c          425 g          547 t
ORIGIN      
        1 ttgaagaaag gatttttgag agatgatata gtaggtttta tttttagtta aaatagggga
       61 gatagaaaaa atgaaaaagt gtagaaatat tattttatca atgttattta ttattactaa
      121 tataacatca ctgattcctg ttcatgtcta tgcggatgca ggcagagata tcagcagtaa
      181 tgtcacttcg ttgaccgtgg acccaacgaa tattacagat ggaggaaaca taaaagtaaa
      241 attctcgttt gacgaaaaaa aacaaaatat ccaaccaggt gattatcttt ggatcaattg
      301 gccaagtgaa ggaaatattc gcggtgaagg attccaaaaa gaaatcccat taatgattga
      361 aaataaaaat gttggaactt taacggtcag aaaagattct gcgcaggttg tgtttaatga
      421 aaatattaaa aacctagact cagtagaagg ttgggggcaa tttgagatac aggccagaaa
      481 cgtaaccgat accggtgaag aaaatacggg accattcacc gtgacgagcg gtgataaaac
      541 agctactgta aatgtgacaa agccagcttc tggttcttct tctagcgttt tttattataa
      601 aacaggagac atgctacctg aagatactaa gcacattcga tggtttttga atattaacaa
      661 taacggcact tatgttgaac aaccagttaa aatcagtgat gaaatccaaa gtggacaaag
      721 attagatcca agtacgtttg aaattaatca aatacactta ggtgaacaga aggtttatag
      781 aggagaagag ggaatccaac aatttttaca agatttcccc agcgctacat ttaatttttc
      841 agtaactgat aattacattg aaataactat accaaaaaac tttgtcaatc ttaggaaaat
      901 aatggtgagt tataagacga tcatagaaaa cccagaacaa ataaattttg agaatcattc
      961 ggaagcttgg tttaaagaat ttaataaacc tgctgttgat ggagaaagtt tcaatcatac
     1021 agttaaaaat atttcagctt ctggtggagt caacggtaca gttagaggcg aattgaagat
     1081 tttcaaatat atcaatgaca cagagattgg tatcccaaat gtgactttcg aattaagacg
     1141 agcagatgaa cagcctattc aaggacagtc tagtatcctg ttgacttcga atgaacaggg
     1201 agaagctagt atcaaaggcc tgcaagtagg agactatgtt gtaaaagaaa aagaagcacc
     1261 caattggatt gattttgatc cactaagttc aaatgaactc aaattctcca taaacgaaaa
     1321 tgatacagag ggagtcagtt tacctattta taacaagaaa aaggttacga acatcacagc
     1381 aactaaaatt tggaacggtg gaaccactcc aagaccgagt atttatttta aattattcag
     1441 agcaagtaca aacaattggg aaccagttcc tgatgcagaa acaaaacgat tagacaatgg
     1501 aataacttca gtgacttggg aagatatcca acagtatgat gatagtggaa acgaatatac
     1561 gtttaaagtg caagaagtag atcaaaacgg aaatgattat gtcccttcag gttatcaaaa
     1621 aattgaaaac gggctagtcg ttacaaatga gaataaagaa gtcatttcag tctcaggaca
     1681 aaaaacatgg gaagacaatg agaaccaaga cggcaaacga ccgacgacga tcacagtaaa
     1741 tctattagcc gacggtcaac cgatccaaca caaagaagta agtgaaaaag atgattggcg
     1801 ctatcgtttc acaaatctgc caaaatacag agacgggcaa gaaatcatct atactgtaac
     1861 ggaagacaac gtgcccgaat acagtacgac gattgacggg tataacatca aaaacagtta
     1921 tacaccggaa aaaacaaata tatctatacc aggtagccat ataacaccgt ggaaaaattt
     1981 tccagagaag acagagagag agaaaattag aaatctattc tctcaaaaat tgcaaatatt
     2041 tgctcataca ggagaatcca gcgaacaaag aagcaattat attagtaaga gagctgtacc
     2101 taaacagata acaaataaat caaaaagcat tcttcctaaa acaagtagta aaaaatctag
     2161 ttttgctg
//
LOCUS       DAZUKC010000178         2123 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000178 DAZUKC010000000
VERSION     DAZUKC010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.185"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(76..501)
                     /locus_tag="ACG80T_002790"
                     /pseudo
     CDS             complement(76..501)
                     /locus_tag="ACG80T_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
     gene            complement(610..1641)
                     /locus_tag="ACG80T_002791"
     CDS             complement(610..1641)
                     /locus_tag="ACG80T_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4842388.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          651 a          344 c          366 g          762 t
ORIGIN      
        1 tatgcgtttt tttgttgatt taccaacatg tttagacatt ttcctctagg aaaaaattaa
       61 ctagcaccac gggttaggaa tctgttttga ttttgttcca tttgaaattt ctctcactcg
      121 cttttttgtc tcatcatcaa ataaaatgaa agggtcttct aaacgatcga taaatacttc
      181 cattgttgtt gttggtagat gataagaagt catgacaggc actgcaccaa ggtaagacac
      241 agaaacagct agaaggtatg tgtcaaaagc ggaactctta taaataataa ctttgtctcc
      301 ggatttcact ccaagatgag ccaattgata agcccgtttc aacacacttt cgcagcattt
      361 tttgtaagtt gtttcaagtc ctaattctgg gaaagctgaa agtggctcat caaagataat
      421 cggggtctcg gaaaagttag ctgcagcttc ttgaaaattc gtaaataagt tcaaagggga
      481 atacgttatg gtctccatca ttcacactcc attatctttt gttaagtaat atacaaattc
      541 aagataatcg taacataatc ctttatataa taacatagtt tattgtgaaa aaaagatcat
      601 acatttgttt tatatggaag aaacccgaac gaatgccgct aattcgtgat aagaaaaatc
      661 aaccatcttt tcccagatat cttgaaaaga atgggatgag aggtatcgat taccacataa
      721 ataatttacc gcgtcagcat catctaaata tagttctgca taataacgat atgtttttct
      781 gcatagttct ggcgatacgt ttttatatac tgtaattttg ctcaaaattt ggcgaatagt
      841 ccgaggagaa atttgctgat catgtgagga acgaaaaaca aaagaatttt cgtccgtatt
      901 ttgtgctgtt aacttaaaat agttctccag aaatggcatc gcttcttttg gtattggtac
      961 tttattcagt ctgttttctt ttctattcag attaagaatc tgcttggtca aagatacatc
     1021 tttacgttta agttctacta cttcatggat ctttaatccg caatacagaa gaatggcagt
     1081 aattgcaagg ttcctcggtc tattcttttc ttcaaagcta gcttctctgc ttgtttgaaa
     1141 gccttcaatc gggataaaca aatgctcaaa atctttgaaa agttctgcat cgatatatcg
     1201 cggagctgct ttggttgttt ccggtaaact tgtacccaaa agagaaatct cttcttcaat
     1261 cagtcctttt tgatacaaaa atcgtaaaaa atgatttaat gaatattgct tccttgaaat
     1321 cccgtaatag ctattttcaa acaattgctc cgtttcttgg ttcgctgtag tttggaacga
     1381 acgagtatgt cgatagattt tagctaagaa attctgataa tcttgttttt ctaaatggat
     1441 aatcagctga tcatccactt cttcagaaaa tacttttctc aaataatcag taaacagatg
     1501 aaaatctttt gcgtattcat agcgactgcc taatgaaagg gaagaacgat tttcttctaa
     1561 aaactgttga aagacggaag ggagcaagtg tgtacgttgc tttaactttt cttgcagttg
     1621 ttttctagtt ttgctgtcca ttacaaattc cctccaatag tgttcatttt ctatatttta
     1681 ccgcatttca tcaaatgaag atagacagtt tgtcttgatc ttaggaagca aatttttaac
     1741 taagtactag ctaaaagata tgaagtaaga agatacattt ttagttattt tattagtgca
     1801 gtcattcttt tgatctttta attgatttat cctcttttta gctatgtggt tacttcatga
     1861 gcaatgaaat ggatttggtt cgttctaatg gcaggaggct ctttctttta tttccgaaaa
     1921 attagaaaac attttttcag aatcgattgg ctctccattt attcaataat ggtttgtcta
     1981 acgatattgt tgggataatt cttaaagaag tattagaaat agttggtact tcattacttt
     2041 acatgaagat ttattctatg gtaggagcaa ttatattgag aattaatcct tttttatata
     2101 agttgtttgc ttattgatgg att
//
LOCUS       DAZUKC010000179         2121 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000179 DAZUKC010000000
VERSION     DAZUKC010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.186"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            136..309
                     /locus_tag="ACG80T_002792"
     CDS             136..309
                     /locus_tag="ACG80T_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842389.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            949..1422
                     /locus_tag="ACG80T_002793"
     CDS             949..1422
                     /locus_tag="ACG80T_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFS4842390.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1431..1657
                     /locus_tag="ACG80T_002794"
                     /pseudo
     CDS             1431..1657
                     /locus_tag="ACG80T_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          686 a          341 c          314 g          780 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa
      901 taattattct gctatatttt agctatcaaa gataaaagag ggataaaaat gaaatttaaa
      961 acattgctat taaaatttgt cgttttaggt ataagtgccg tatttatttt gttcggcgta
     1021 cttttttata ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca
     1081 ttatttgttt ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta
     1141 ttgaaactat tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata
     1201 ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct
     1261 aaatttatga aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct
     1321 gtcgtattca ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa
     1381 catgcgattt tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta
     1441 tcgttaattt agatgtagta ttagctcaaa aaaaatgagt gtgactgaat tgagtgagaa
     1501 agtgggcatt acgatggcaa acatctctat tctaaaaaat ggaaaagcaa aagccatccg
     1561 tttttccact ctcgaaaaaa tctgccaagt tttggagtgt caaccaggtg atttgttgac
     1621 ctataaaaat gactcgaata ctataccaga agaataaaat aaagtagaac aaacagtaaa
     1681 taaaaaataa tctgaatgat agatgtgtga gacttttaga agttatttac gtctattatt
     1741 cagatttttt atttcgattg aataccgttt tatctataaa taagatatgt actgactcat
     1801 acaaaggggg ccttttcttc tccatgtact atcacttttc ctgatacaac tggtttcctg
     1861 ttacgttgta gttggatctg cttttagtat aatactttat tcattccacc agactgatag
     1921 cagtttgagc agtctaaata atcatgtagt attacaggcc attctgtttc ataccttgaa
     1981 gtgcttaaaa cttcagctaa tggcccattg tgttaactct tactccattc ttttttagta
     2041 agctcccata tatactgtaa agccttctat atattatgtc atgttgcttt ttaacattta
     2101 ctatgtagtt ttgataaaat g
//
LOCUS       DAZUKC010000180         2075 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000180 DAZUKC010000000
VERSION     DAZUKC010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.187"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..334
                     /locus_tag="ACG80T_002795"
                     /pseudo
     CDS             1..334
                     /locus_tag="ACG80T_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324676.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(606..896)
                     /locus_tag="ACG80T_002796"
     CDS             complement(606..896)
                     /locus_tag="ACG80T_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842391.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1104..1795)
                     /locus_tag="ACG80T_002797"
                     /pseudo
     CDS             complement(1104..1795)
                     /locus_tag="ACG80T_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          588 a          388 c          307 g          792 t
ORIGIN      
        1 ttgatgcaaa agaaatctac gatagtcaac aaaaaaattt tcattatatc cagaaaaatt
       61 tttcttagtt aaggacctat ggattgcagt aatgcagaat tcgtcgaata aactgccaaa
      121 gacatacaat catattgctt ttaaaattga cgaacaagat atagattcgt ttgtctcaaa
      181 aatccaaatg ttaggtttaa ctgtcgaacc aggacgttct cgtgtgaaag gcgaggcttc
      241 ttcaatttac ttttacgact atgataatca cctcttcgag ttacataccg gtactctcca
      301 ggaacgttta aaatcctaca attccactac ctgagactag taatgactag tctcttttta
      361 tggcctctga tccccccatt gagcgaatac taatagggct tcgaattgat ctgttcaatc
      421 tttaagcaat atccctcaaa tgagtttgga aagtttagtg attcatcaca aatacttaaa
      481 tacatgtagt tatatataga ttgaggttct tgtgagcgta tgctcgtgtg ggttcaaatc
      541 ccatcgtctg catccatctt tccaaaacaa gcggctagga catttttgtc tcagccgctt
      601 gttttctagt ttattctttc atcttgtcgg accttttttc ttgggtatag acttttctca
      661 tattctacca caattttggg tttccctaac acatgatagg atctcacaac aaattgcttt
      721 ctattattat aataacctgt aacgactaac ttcatccctt cgtctacttc agctaggaag
      781 ttcaaactat gggaagcaat caaacaattg accccattta aactgaaacg gatcaacggt
      841 ctggcactaa cttgcaaagt acggatctta tctactgttc catatttttt ctccataacc
      901 ttcacctcaa taccattata cgaacataca ttccctttgt aaagaagaaa ctctgtttta
      961 tctttttgac tttttgtcta ttacctttct taaaatcata ttggcacttc ctgcgaagat
     1021 gaaggacatg tactaaaaaa aagaggcagc agtatagatt actgttgctt ctttactaat
     1081 ttaggtttac ttgttaagaa agattattta cgcaaatgtt cttcaacaat ttgctgagat
     1141 ttatcagctg tatcttccac ttcatctttt gctttttcta acttttgaat atattgtttc
     1201 aagcttgagt tttcagcagt tgcttcgtct aatttctttt gtgtatctgt taactgattt
     1261 ctacatttgt tttttcttgt gtcagtgttg cgatttgttg attcaaacca tttacagtat
     1321 tttgtaattc tgaaagtttt gcttgtgctt cttgtagtct tgctgtccaa tctgcattca
     1381 gatgattgat ttcatcattt ttttggttga tctcatctag tttgacattg atttcatttt
     1441 gtttgctttg tagttgtgca tccttgctga ctaattgact ttctaagttt tgtttttctg
     1501 ctgccgattg atttttttgg ttttctaatt catttacttt tgtttggtat tcttggacat
     1561 ctgcctttga atcttgcaat gtctgttctt tttgtgctag tcgagcggta agatctgtaa
     1621 tcgcttgctc tttatctgtc aatgtatgat caagctgtgt cagattctca ttgattgtat
     1681 caatatctgt ctctccaccc cacaaaataa tttcatttgc gtaccgcgtg cctgtgtagc
     1741 caattagaca tagaaatgca acgacaatcc caccagtaat aaatttcttt ttcatttgca
     1801 tagctcctta atccaatact tttacatgct aaaagtaagt gatctcaaac acaatgtcaa
     1861 gcgtttttaa attacatatg taattttaat ataaatacta tcaaatactc atttaatcct
     1921 ccttttttat ctatttaaaa aatctttttt atagagaaaa atgcaggtta atactttctt
     1981 aaataaatat ttatggtgaa caaaaacaaa aaagcaagtt ttattaaacg taaccctttt
     2041 catttcatag attactgtat ttattacaaa aaacg
//
LOCUS       DAZUKC010000181         2063 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000181 DAZUKC010000000
VERSION     DAZUKC010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.188"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..914)
                     /locus_tag="ACG80T_002798"
     CDS             complement(<1..914)
                     /locus_tag="ACG80T_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFS4842392.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="ACG80T_002799"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="ACG80T_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFS4842393.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT          688 a          370 c          402 g          603 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agc
//
LOCUS       DAZUKC010000182         2036 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000182 DAZUKC010000000
VERSION     DAZUKC010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.189"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            200..742
                     /locus_tag="ACG80T_002800"
     CDS             200..742
                     /locus_tag="ACG80T_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842394.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..1347
                     /locus_tag="ACG80T_002801"
     CDS             898..1347
                     /locus_tag="ACG80T_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFS4842395.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMATIALDNEHPRI"
     gene            complement(1377..>2036)
                     /locus_tag="ACG80T_002802"
     CDS             complement(1377..>2036)
                     /locus_tag="ACG80T_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HFS4842396.1"
                     /translation="RIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQ
                     PLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLT
                     DVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQY
                     QFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEA
                     A"
BASE COUNT          733 a          320 c          389 g          594 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgg aatggcaaca
     1261 ctttttttga caaaaattat aaggtgttgt gaatgctgcg gtgttatggc tacgattgcc
     1321 cttgacaatg agcatcctcg gatctgattt tctccggcag aagcacaatc attcaattac
     1381 gctgcctctc cagaggtatc agatccgcgc tcattatcaa ggattcgctt cgccaatctc
     1441 tgttgtcgaa tttggatcgg tgtttcatac gccaacgtgc catgtaagcg tagatagttc
     1501 caccaatgca cataatcaaa caattctaga cgtagctgtg ccagtgtctc aaattggtat
     1561 tgatgcacga attctacttt gacagattta taggttgatt ccacaacggc attatcatac
     1621 ggacaaccct tcctactcaa tgaacgagta attccaaaac cattcagaat ctcatgaatg
     1681 gtttggttat caaattcctt tccccggtct gtatgaaaaa gcttgacgtc tgttaaagaa
     1741 tacggtatcc gtccaaaggc ttcttttacc aatgaggcat ctttcttctc accacaggaa
     1801 taaccaataa tttctcgatt aaacaaatca agtattaagc agatataatg ccactttttc
     1861 cccacgcgaa cataagtaag atccgtaacg atggcttcca atggctgttc ttgtgtaaag
     1921 gtccgatcta atacattcgc tgttttcgct tcattacaag ctgttcgttg ccctttaaaa
     1981 tgagcgatcg tataggtaga tgtcaatccg cggcgtttca tgattcgacc gattcg
//
LOCUS       DAZUKC010000183         2036 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000183 DAZUKC010000000
VERSION     DAZUKC010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.190"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            649..981
                     /gene="traE"
                     /locus_tag="ACG80T_002803"
     CDS             649..981
                     /gene="traE"
                     /locus_tag="ACG80T_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFS4842397.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1600..1941
                     /locus_tag="ACG80T_002804"
     CDS             1600..1941
                     /locus_tag="ACG80T_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFS4842398.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          710 a          271 c          428 g          627 t
ORIGIN      
        1 gaaataatta atttcaaata tagaaaaggg aacgtatgtt tgcatactcc ttttttttga
       61 gttataataa tacgcgaaga gagataattt cattatttaa acaagctaaa aatcttgata
      121 ttacttcttt tcaggcaata cgaatattat tcgcttacat tttttgtata aatttaattg
      181 tctgctatgc ttgtgatgaa ttgattttcg ttttgcactt ttggatgata aatttggaca
      241 attgaaaata caaaaaaaag aaaggcggta tgaactcgga aggctggtaa ccttcgtcag
      301 cgttcataca cggcattaag atttggccct taatgcgtta acctttcagt gttacggtat
      361 tacccgcaat ggatttagta taacagatat tcaaaagtga taccactttt tttggctgtg
      421 ttaaggttaa aaatacgtga tattacgatg aatctatgcc taagcataat gttataaaaa
      481 tgtccgtgtg gctctttgcc gtataacgaa cgctgaaatt agagggaacg catgtttgga
      541 tgcgttccct cttttttgtt tgtcatgaac gaaaatcaat tctctatttt tgaaaaataa
      601 caagaaaaac ttgttaaaaa tatttaaaga aattgggtgg tttattttat gtcatataca
      661 ggaattttga gtctcaaaga tatttgtcac tacggtaaac ggtgtaccgc tacagagaaa
      721 atcacgaaga aactatcaac tggccaaagt aaaaccgttg tgcagtgtaa gaaatatata
      781 atccagaaag acaaagtctc agaggaaatg atttattaca ttgggaaaca aaagcaaata
      841 attttaaaag atcctatccc cttaaaagag ctatatccaa caatcaaaca tgtttatgac
      901 caaaacggtg tactgattgg tcgaagaaaa aatggagtct tgcgttgtac cgcaaaaggg
      961 atggggcgtt tgatcggctg aattgatcat tctcataact tgtattttat cgtttttcgc
     1021 ttgcttgaaa atattgtcga gacttctaaa ggaacttaga gtcactagaa aaataaggag
     1081 tagacttgat ctatcaacaa ttcccttcgg ggagtgacgg ggtaggtttt aacgattggt
     1141 aacgtaaaaa attggtcaat cgagtaaatg cgcttgtttg gatagtggtg aaatgaattt
     1201 gtgtaaataa taagcgcaga gtaaacaaga gggaatttag aagttagaca agttagaata
     1261 gcgtctagat taatcatgag cagctactaa gggatctaga ctttgacgca aactcatgga
     1321 ataaggagta ggtcttctcg taatggggag gacgcacaag aaattgtgtc gtgtgctgca
     1381 gtacatgtag atacttgcga ggacgttgat tattggatca atcgagcatt taccgctgtc
     1441 tactagggtt tctcgtttcg gacgccataa acgagtcgaa attttttatg tatttttaag
     1501 taaacgaaaa acgataaaat atgaacaagt cctgaataaa gtgaaaaaat taatataaag
     1561 catagagaaa atgaaattta cgtaatggag gaatgacaaa tgaaaaatta tgatccgaat
     1621 atacgattgg gcacacatac tattaaggtt tctttccaac gttgggatta caaaggattt
     1681 gtgacgttta gaaggggcgg aaattgtaaa ggattagatg ttttagcatt agacgaggat
     1741 gatttatacg atcaaacatt gactgataat ccaattggat tcggcttgct acctgaagat
     1801 gatgaaggta atgaatggtt taaaatgacg cttatgaatg ataatggaga cgaattatct
     1861 gttgaagata cttggtcata tttgagtgat tatattgtca gctttgaaat cattgaattt
     1921 gtagcggata aagaagaata aaaactggag gaataaattt tacagataaa ggcttagaag
     1981 gagtcacgac gattaataaa aaggtagtat taccttaggc attgagtaag gaattt
//
LOCUS       DAZUKC010000184         2002 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000184 DAZUKC010000000
VERSION     DAZUKC010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.191"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..377
                     /locus_tag="ACG80T_002805"
     CDS             <1..377
                     /locus_tag="ACG80T_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFS4842399.1"
                     /translation="NVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFRR
                     FPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTDD
                     WQKVMAAEVIATYLDGDLVYKK"
     gene            425..1825
                     /locus_tag="ACG80T_002806"
     CDS             425..1825
                     /locus_tag="ACG80T_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFS4842400.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
BASE COUNT          554 a          339 c          432 g          677 t
ORIGIN      
        1 aaaatgtttc cacagaggac ttctttaatt attgtcgatt agaggaaagt ggtgtaacag
       61 tcactagcgg tacagattta ccgctgttta ttactgatat tcctgaatcg attataagag
      121 cagtattccg tcgttttcct gaaggagaag aagtctggga gaaagaaaca agcttcacga
      181 caaccgagct gctgaaaagt tttacaagca acagcttcaa agtaaatggt ttatcggagt
      241 acggagaaat aaaagtaggg aaaagcgcca gcttagctgt tttcagcgag gacctctgta
      301 ctgatgactg gcaaaaagtg atggctgcgg aagttatcgc tacctatctt gatggtgact
      361 tagtttacaa gaaataatgc ttttcgatat aacgttataa aacgaattaa agagaggtta
      421 aaaaatggaa acaatgaaaa gaaaattcac cttcccacat agttatacat tgatttttat
      481 cttaattatt ttggctgcaa ttttaacttg gataattcct agtggtcagt ttgagacagt
      541 tgttgaaaac gtcgcaggta tggaaaaaac aacggttgtt ccgggaacct atcaccaaat
      601 cgataaggca gattatggac aaggtttttc ctctatacta atggcaccag ctgaagggat
      661 tatttcagct gttgaggttg ttgcttttgt tctcatcgtt ggtggtgcct tcgggattat
      721 cctaaaaacg ggagccatcg atcgagggct gtttacctta gcaaaagttc tgggagacaa
      781 ggggatctta gttattccga tgtcgatgat tctattcagc ttaggaggat caacctttgg
      841 catgagcgag gaagtcattc ccttatttgc ggtgtttatt tctctgatgt tttctcttgg
      901 ctatgattcc atgactgcga tcttgattct atttttaagt acacaagcgg gatatgttgg
      961 gtcgacgatt aatcccttca acgttttaat cgcacaggga gtagctggcg tttacggaaa
     1021 tcccctgctt tggtttcgga tgctatcctg gattgtcatt actgggattt caattgtttt
     1081 caccatgctt tatgctagca gagtcaaaaa gaatccactg tcctctattg tttatgagag
     1141 tgatttaaag gttaagcata agtttcaaaa ttttgacact gaaaaagagt tttcaacaca
     1201 agataaacta attatcgccg gttttatcgt aggtttagga atcatgattt ggggtatcat
     1261 ttccaaggga tggtatatgg ttgaaatttc tgcactattc ttaggattag gtctttttgc
     1321 tgggggcatc gctagatttt ctcaaaagga aattgctgaa gcttttgtcg aaggctgttc
     1381 agatttcgcc tatgctgctg taatcattgg gctagctaga ggagtcttgg taatcttgga
     1441 aggtggaatg attattgaca ctattctaaa cagcttggct aaccttttag gtggtcttcc
     1501 aaaattcctg ttcactacga ttctactcat ttctcaagta attgtttcct tttttgttcc
     1561 atcctcctca ggagctgcgg cattaacgat gccggttatg gcaccattgg gtgatctaat
     1621 gaatgtaagt cgtgatgttg ttgttctaag taaccagttt ggaaatggtt taatgaatat
     1681 tatctcccca actagtggtg tattactggc aggattagcc atcgcagaga ttaatttctc
     1741 taaatggttt aaggttggct tcaaaatatt tgccatctta acccttgttt ctgctgtttt
     1801 gttatacatt gctacactct tttagtctta atcatccatt aataggaggc aatagttatg
     1861 attaaaataa atcaattagc aggaatgaat atccactatt tattttattc tctcaaagaa
     1921 tttttcgctt atcaacagaa aattggtatt tcttctgttg aactttgggg aggtgctccg
     1981 cattttttca tagatgcttt ta
//
LOCUS       DAZUKC010000185         1979 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000185 DAZUKC010000000
VERSION     DAZUKC010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.192"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(346..528)
                     /locus_tag="ACG80T_002807"
                     /pseudo
     CDS             complement(346..528)
                     /locus_tag="ACG80T_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(728..1744)
                     /locus_tag="ACG80T_002808"
     CDS             complement(728..1744)
                     /locus_tag="ACG80T_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS4842401.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVQGT
                     TTPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGINGWQ
                     LFDQYGEDIAYIEILKFHEGKGRIDFNPNKIGQFLASSMKGFIHDLFLDPHFSRADIA
                     CDIVDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWGSRASERQVRLYNKKLEQERK
                     KVIVPKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFASPHFLPIDIKPIDKIVIDGL
                     IAEPSNWSIIARHTKYKYRNLLKQESQNDELTNHLRETFKESADELKKELDTWLLGLD
                     VTEK"
BASE COUNT          559 a          459 c          308 g          653 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggttttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cacgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaaca ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaatgct ctccgagggt attcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt cacgcaaaat aatcgcttat
      661 cgtaaatcca cgtcactaaa cattgacgta aatttactct cactcatctg acttttctta
      721 ttcggtatca cttctctgtc acatccaacc ccaaaagcca cgtatccaat tcttttttta
      781 attcgtctgc agattcctta aaagtctctc gtaaatgatt cgttaactca tcattttgac
      841 tttcttgctt cagcaaattc cgatatttat attttgtatg tctagcaatt atcgaccaat
      901 tagaaggctc agctatcaaa ccatcaataa ctattttatc aataggtttt atatcaatcg
      961 gcaaaaaatg cgggcttgca aaactatcta agctttcctg aaccatcgca tgccaatccg
     1021 ttgctttgcc acgtcttagc tgcatttcta accgccacca cgtatcgatt tcttttggca
     1081 cgattacttt ttttcgttcc tgctctagct ttttgttgta cagtcgcact tggcgctcac
     1141 tggcacgact gccccagtac gctgtttcta gcttgccaga acggccgtag attggcttaa
     1201 aagaaatcgg gtctacaata cgatactgag taataaagtc atccggcaca tctacaatat
     1261 cgcaggcaat atccgcccgg gaaaaatgcg gatctaaaaa taggtcatga atgaatcctt
     1321 tcatggaact agctaaaaac tggcctatct tattcggatt aaaatcaata cgaccttttc
     1381 cttcatgaaa tttaagtatt tcgatatacg caatatcttc tccatattga tcgaataatt
     1441 gccagccgtt tatcccaact gatttcaaat atccctgatt ttcgttcaaa cgcattaatt
     1501 gctcgaaatc gacaaaaaca actccatttt tcgtccaata agctatggta tcattcagtt
     1561 ttccgacaat tgtaattcga tcaatcgacc aatttaattt taggggggtg gtcgtacctt
     1621 gcacacttaa ccgcctgtta gaaatacggt taagtttttc gagctgattt atcgttgttc
     1681 ttttttctgc ccctcgacct tcaatttttg attgaatttc actcaactga ttttcatttg
     1741 ccattttcgt cctccaaacg aaaaaagaga cttttggaaa gaatttccag agttcacgta
     1801 accctaaaaa ttcatatcca aaaatctcta gcttgaagaa acctaaaaat attcgtataa
     1861 tgaacctgag ttacggagct aggtgaagta gtttccgtaa atagtcgaga gtgttagcgc
     1921 actgctcggc ttttattttg ctcttttttc tcgttttctt tgattatagc actcctcct
//
LOCUS       DAZUKC010000186         1911 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000186 DAZUKC010000000
VERSION     DAZUKC010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.194"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1412
                     /locus_tag="ACG80T_002809"
     CDS             <1..1412
                     /locus_tag="ACG80T_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFS4842402.1"
                     /translation="PTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTN
                     TPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNV
                     TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNA
                     DKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTY
                     PDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYT
                     WKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTG
                     KTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNV
                     VVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGL
                     ILLGTLGLLGYKNRKKENE"
     gene            1546..>1911
                     /locus_tag="ACG80T_002810"
     CDS             1546..>1911
                     /locus_tag="ACG80T_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4842403.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPG"
BASE COUNT          764 a          275 c          402 g          470 t
ORIGIN      
        1 aaccaacagt agaaggcgaa aaagtagaag ttgggggcac agtagatcta acagataatg
       61 ttactaactt gccagcgtta ccagaaggaa caacagtaac agatgttact cccggcggta
      121 caattgatac caacacacca ggtaattacg aaggtgtcat tgaagtaacg tatccagacg
      181 gtacgaaaga tacggtgaaa gttccagtag aagtgacaga caatcgttct gacgccgata
      241 aatatgaacc aacagtagaa ggcgaaaaag tagaaattgg aggcacagta gatttaacag
      301 ataatgttac taacttgcca gcgttaccag aaggaacaac agtaacagat gttactcccg
      361 gcggtacaat tgataccaac acaccaggca attatgaagg tgtcattgaa gtaacgtatc
      421 cagacggtac gaaagatacg gtgaaagttc cagtagaagt gacagacaat cgttctaatg
      481 cagataaata caatcctgaa ggacaaaaag tgactacaga cttaaataaa gagccagatg
      541 catctgaggg aattaagaac aaagaagatt taccaaaagg aactacttat gcttggaaag
      601 agaaagtaga tgttagcaca gctggaaata aaaaaggtac tattgtagtg acatatccag
      661 atggatcatc tgatgaagtt gaagtagacg ttacagtgac agatactcgt tccgatgcag
      721 ataaatataa tcctgaagga caaaaagtga ctacagactt aaataaagag ccagatgcat
      781 ctgagggaat taagaacaaa gaagatctac caaaaggaac tacttatact tggaaagaaa
      841 aagtagatgt tagtacagct ggaaacaaaa aaggtactgt agtagtaaca tatccagatg
      901 gatctaaaga agaagtagaa gttactattt ctgtagtaga taaaaaagca ccgaataaac
      961 ctcaagttga tcctattaca gagggtgacc aaattgttac tggtaaaact gaaccaaatg
     1021 cagaggtgac agttacatta ccagacggaa gtcaacacca tgggacagct gataaaaatg
     1081 gtaattttac agttaaagtt cctaaattag aggcagggac aaaagtgata gtaactgcaa
     1141 ctgatgaatc tggtaatact agtgaaccta ctaatgtagt tgtttcttcc aacaaaaaag
     1201 acagtggaaa aaacggtagt aaaggtagta agactgacaa tcaaggtagc aacagcaatc
     1261 aagacaaaaa tagaggcaaa agccagtcct caaaagtttt tcctaagacg ggagagagtg
     1321 acagtaatat ctttactatt agcggtggtt taatactgtt aggaacgtta ggattattag
     1381 ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa tttcttaagt cttctctgag
     1441 aagaggaaga atctttttct tcctcttttt gcttttttat gatgggaaat aagtgttagt
     1501 taaagatgaa tatacttttt atagaaagga aaagaatgta attaagtgga aaaaatagta
     1561 attattggtt caaaccattc agggctagcc gcagctcgat ttctaaactt aagcgattca
     1621 aattatgaag ttattctaat cgataaaaaa tcagaaatta gttacttgga atgtgggaca
     1681 tcgttattta taaaaggaga agtaagtaat ttagaagatc ttttttatca aaatagaatg
     1741 acactaaatt ctgagataaa aaagttattt ctagagacag aagttgacaa aattgatatt
     1801 caaaataaaa agattagttg tgtaaaaaaa gatggtacaa gattctctca agaatatgac
     1861 aaagttatat tagctacagg ttcaaagcaa attatgctag atataccggg g
//
LOCUS       DAZUKC010000187         1858 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000187 DAZUKC010000000
VERSION     DAZUKC010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.195"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1822
                     /locus_tag="ACG80T_002811"
     CDS             <1..1822
                     /locus_tag="ACG80T_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842404.1"
                     /translation="NKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSPADSNQMKE
                     IKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWI
                     TVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVY
                     LGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFAL
                     ALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTF
                     QLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPW
                     IVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVD
                     FLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEE
                     AKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHA
                     LEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQER
                     FDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT          606 a          344 c          313 g          595 t
ORIGIN      
        1 taataaaaaa gaatcttttt atttacgtat aaagtggagc ataatcatta tattatcatt
       61 gttattttta acaggaattg cttatttctt ttattttatt ttttctcctg cggacagcaa
      121 tcaaatgaaa gaaataaaag attcgtgggt tttctataca aaagatgatc ctgattttaa
      181 gtttgacagc aagtacactc gtacgattcc taccgtggat aaagacgaaa cattcattat
      241 ggaaacgaca cttgaaaaac cgttggacga agcaaattta ctaatcaaag gaaaccatca
      301 atggattact gtatatctag gcgaaaagat tttataccaa cggaaagatt atcaggaaga
      361 aagcaatccc ggactctcat tggctgtgat agatttacca gcaaattata tcggaaaaaa
      421 attgaccctt acagtctcat ctccttatca gaactatgct ggactgccac cgaaagttta
      481 tctaggaaca actggaccat tgattagttt tatttattca caatcagtgc cgcaagtatt
      541 gacgatgata atcgctgttt ttatcgctat agggaccttt ttctatacga tctacttgat
      601 gtataaacaa aaacgtcttg aatactcact tattctctta agttgctttg ctttggcatt
      661 aggatttgaa gctgtatcag aagatatttt aagtggtatt ctttttgaac cattcgttca
      721 ttctttttta tctcatttat ttattatctt aacgtcagct ttcatcatct gctactattg
      781 gagccgaatg ttttactata aaaaatggta cggaattttt tgtacgttcc aacttttgat
      841 ttttatcggc gttctgcttt atgcaacttt tactcctgca gaacttcctg aattaatgcc
      901 gattgctaat attgctagtg ttataagcac tttagtcact tcacttgcat gcattggcga
      961 agcatataag aataaccgtt tttatgtagc ttgtacacct tggatcgtct tggtagccat
     1021 tatccattgc ttgatttaca tccaaacggc attaggtatc tatcaaacaa cgatcaattg
     1081 gtctacgatt ttatatatta tcattctaat cgttattatc agctacaatc taatcgatca
     1141 catcttaaat accgacaaag atcggagaca agtagatttc ttaaaaatca aaaatgattt
     1201 gttggagcaa cattatgagc agcttcgaca gcatatccaa gaagtaaata cacttcgcct
     1261 ggaatttgtc caagagatgg agaatctgca agatttaatg catacggacc aaacaaaagc
     1321 taaaaactat gtggaaaaga tattagaaga agcaaaaaat ttcgaaatgc tgtgttcttt
     1381 ttgcaaccac caggtgacaa atcttatctt agcgcgttac cagaagttag ctgccaaacg
     1441 gaatattcag ataaccttcc aagcagatct tcccgaagaa cttcctatca acgatgacga
     1501 tctggctcaa cttttgatcc acgctttaga gcattccttt agagaaactc atgccatcga
     1561 aaatccactg tatcgaaaaa tttatttgtc gatacacgaa caagaggaac atttcgtcat
     1621 ttgttgcgaa catagtgcac attacgatac aaacctattt agtcgaggca tcacagaaga
     1681 attagatgat caggaacgtt ttgacttaat gatgatgaaa gacgtagcgg atcgttacac
     1741 aggaacacta acgcaggaaa aagatacttt cattgatcgg atcactgttc aactctctcg
     1801 aaattacccg aatcctgtat aaaaaatagc ggaatccata tatgtggatt ccgctatt
//
LOCUS       DAZUKC010000188         1827 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000188 DAZUKC010000000
VERSION     DAZUKC010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.196"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      198..364
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            538..1644
                     /locus_tag="ACG80T_002812"
     CDS             538..1644
                     /locus_tag="ACG80T_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFS4842405.1"
                     /translation="MIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLSWSVIFLAR
                     SVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQFQPSELANL
                     FLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIAWVIFWAAA
                     VPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDSFIDSHGAG
                     YQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIGALCVLFLL
                     FSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGVPLPFVSYG
                     GTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
BASE COUNT          544 a          294 c          372 g          617 t
ORIGIN      
        1 tataaaaaaa cacataaaac taaaaaatta tcaaagaatc ctggtgcaac tgaaagaatt
       61 tgcaacggga ttctttcaac ttatttgtta tttttttcta aaaaatatta aagattgtta
      121 aatttcacta tttcatttca aattccattg cctaatcatt aggaaaagtc tatcatgaac
      181 tcgtaaatag aatatagtaa taaccttgtt aggtgaggct cctatacaaa cataggctgt
      241 tgcccagaaa cgtcgaaagt cgccaatggg taaaacagaa actgttggat taaggctttt
      301 tccaatagct aagaatgttt tctttacgtt gtatagtgct aaaactcgga cgatgagatg
      361 atgctaggtc agaattcttg ttcgaaaagg tttctgacct tttctttata tttttgtgac
      421 tgacttattt gatgagacaa actcgctcct cacctgacaa gtggattgct gtgtgaggag
      481 gagatgcggt gaaaaaagat cgattggtgg attttgggtc cttatttgac tttgtccatg
      541 atcggtcttt tagaagtata cagtgccagt tcttatcgtt tattgcaagc cgatgaaaat
      601 acaaaaagtc tattattgcg tcaactaatt ttcatatttt tgagttggag cgtgatcttc
      661 ttagctcgtt cagtcaaact acactattta cttcacccta aaatagcagg atacggttta
      721 gccttatcga ttttcttttt agtattagta agaataggga tattcggtgt cactgtcaac
      781 ggcgcacaac gttggatctc tctgtttggc attcaattcc agccttctga actggcaaat
      841 ctttttttga ttttttattt aagctggttt tttcgtgacg gaaatagtag cccaaaagat
      901 ctaaaaaaac cattcctgat tacagtaggt ataacttttc tgattttatt tcagccaaag
      961 attgctggag cattgatgat cctttcgatt gcgtgggtca tattttgggc agcggcggtt
     1021 ccatttaaaa aagggatcta tctaatcgtt actttttctg cattgctgat tggagcagca
     1081 ggcggggtat tatatttagg aaataaaggt tggcttccac agatgtttaa tcatgcttat
     1141 gaaaggatcg cgactttaag agattctttt atcgatagtc atggtgcggg ctatcaaatg
     1201 acccattcgt tttatgcact atataatggt ggcatatttg ggcggggatt aggaaatagt
     1261 atcacaaaaa aagggtatct tccagagaca gaaacagatt ttattttttc gattattaca
     1321 gaagagcttg ggctaatcgg tgcattgtgt gtgcttttct tattgttctc attatgcatg
     1381 agaatctttt gtttaagcag tcgctgtaaa aatcagcaag ctggattgtt tcttttagga
     1441 ttcggaactt tgctattcgt tcagacgata atgaacgtgg gaagtatcgc ggggctgatg
     1501 ccaatgactg gtgtgccatt gccatttgtt agctacggag gaacaagtta tctgatcctt
     1561 tctttaggaa tcggaatcac gttgaatatt tcttcaaaga tacaggccga agagttggcg
     1621 ctctatcgac ctgaaaaaca ataaagagaa actgagaaac tctcagtttg aattatcata
     1681 aagtacaatc ttttattttt caagcgagag gttcttttag aaagaggaat tcgcaatgaa
     1741 agtatctcaa aaaatcatat tatacccgtg gtgctagtta attttttcct agaggaaaat
     1801 gtctaaacat gttggtaaat caacaaa
//
LOCUS       DAZUKC010000189         1703 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000189 DAZUKC010000000
VERSION     DAZUKC010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.197"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            534..1574
                     /locus_tag="ACG80T_002813"
     CDS             534..1574
                     /locus_tag="ACG80T_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFS4842406.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          665 a          213 c          256 g          569 t
ORIGIN      
        1 actttaaagt aatttttttc agattaacac agtagtatct tattaaataa gttaatttta
       61 atggtagtac ataattatta tatatatctg acattggtag tacttattgt attttttagt
      121 agtatataat ctattgtttt ctagcctaaa gccttgtctt ataagaataa tataatatat
      181 actactgaaa taaggtagta tatattacta gtaatggtag tatgttttat atgcataagg
      241 tagttcttaa tttatttttt gttttgacac tggtagtact taattatctt taattttaaa
      301 actaaatttt attcatacgt ttttctggta gtatttaatt ataaataata tttatttaaa
      361 gtagttctta atgactttaa aggtagtaca taattcattt tatttctgat tttaggtagt
      421 atttaattaa aaaacagttt taatgcgttt gcatttttta tttatctttg ctattttcga
      481 aaagagtagg taattttaca taaataaaac aacgtgaaag tagaggatct acaatgactg
      541 attttaaatt ttttaaggcg gatagagttt acaacgaatt attttatcaa tttccaaaag
      601 tctttattgt ttctgacgaa tacaaaaaaa tgaaagattc aactaagatt gcctatatgc
      661 ttttaaaagc aagattagag atcgcaatca gcaaacggca aatcgatgaa gaaggtaatg
      721 tttattttac ttatacgaca aatgaactat gtagagtatt aaactgccaa aaacaaaaag
      781 cgatagcaat caaaaaagag ttggaatcct ttggtttatt attacaaaag cagatgggat
      841 ttaacaaaca gttagggaaa aataatccta atagactata tctagcagaa ttaaaagtct
      901 cagaaaatga tatctactta ctcgaaaaat ttgatagaga gaatagggaa aacgttgata
      961 aatcagaggg tatgaaaatc atacccaccc tcgacgaaaa atcagacgct gaatcccttg
     1021 gggctcaaga gggtatgaaa atcataccgt gccaaaacgt tgataaatca gagggtatga
     1081 aaatcatacc agaacttaat aataatatat tagacactaa tagacacaat atagacactg
     1141 aaaaagaccg cctacaagat caattgttgt tagacaattt tgagacaatt atgacaaacg
     1201 acagcattgc tacgtttgtc cctgaacgag tattaaattt gataaaaaca ttttcttcaa
     1261 gttacagtga agctcaaaaa accgtccaga ctattcataa tgcaaagaaa aaagctgaaa
     1321 tagaaagtga tatttcgata gtttttgaag aactcgatag ttattatgtc aatgcagaac
     1381 aagaattata cacgacactg ttaaaagcct atcaaaaatt aaaaaccgaa aaagtcgaaa
     1441 atatccagaa cctgattttt gtctatgtaa aaaattggtt tatcgaaaaa ccaatagctg
     1501 ctaaagtatc aagtgaaaaa cgtttgaatt atgcaagctc cccaagcact attacgaaag
     1561 actggttaga gtgaaacttt actgacaaag tgattgttgc ttcatggagt aacattaaaa
     1621 ttaaaaattt tttattattc taattatatt acgaatatta caaaataata gctattttaa
     1681 tagaatttat aagttaaaat att
//
LOCUS       DAZUKC010000190         1664 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000190 DAZUKC010000000
VERSION     DAZUKC010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.198"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACG80T_002814"
     CDS             131..505
                     /locus_tag="ACG80T_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS4842407.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACG80T_002815"
     CDS             complement(619..1248)
                     /locus_tag="ACG80T_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS4842408.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACG80T_002816"
     tRNA            complement(1382..1454)
                     /locus_tag="ACG80T_002816"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DAZUKC010000191         1644 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000191 DAZUKC010000000
VERSION     DAZUKC010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.199"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            81..503
                     /locus_tag="ACG80T_002817"
     CDS             81..503
                     /locus_tag="ACG80T_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842409.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            513..716
                     /locus_tag="ACG80T_002818"
     CDS             513..716
                     /locus_tag="ACG80T_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842410.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            927..1511
                     /locus_tag="ACG80T_002819"
     CDS             927..1511
                     /locus_tag="ACG80T_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4842411.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          578 a          230 c          300 g          536 t
ORIGIN      
        1 tttctagtat ataacttgat ttatctgtta agatttatta agaaaacgtt ttcttatttt
       61 tgatttcgag gtgatagaca atgaaaaata agtttatttt atctttaggt attttaacta
      121 ttggaattag tgtaactgga actacaattc ctacatttgc tgatagtaca aatgaaccaa
      181 ctactgtagt agataataac caagaattaa ctcctttaga taggaacgga atagccattg
      241 gtaacccaac taacgactta actgttactg aaaattattt aatagtttct ccattacttg
      301 ctcgagcagc ttctaaaacg atacgagtga caggaacgat gatttttgat aaagttggtt
      361 ggccttctag acctcctcaa tcacggaatt ggaaagaaaa aagaaatggt tattggtact
      421 atggaaaatt gtatttacaa agttataaat cagctggaaa caattggatt gcgacttatt
      481 caggaacctt aactagaaag tagggatttt ttatgccaga atttgtgaac acaccaaatt
      541 tttatcaaga tagcgatatt ctaagtaaaa ttaatttacc gaaagaagaa tcagctttac
      601 aagttcagcg aactttacag gagacacaaa aaattcaaaa atcaatttct aatctagaag
      661 attctgaaga aaaaagaaaa aaaagagacc aaaaaacata tcaaaaagaa aaataatgat
      721 tttaaaaaaa gagtgtctag tcgacattct ttttttctaa ttgaaagtgt tcataaaatc
      781 acccatttct ggacactttc tttttttgct ttatttttca aaaaaacaat tcatttcatg
      841 ttcataaacc cataacaata tctatgttca gttaaactat atggataaag ttatgttaca
      901 ataaaattaa tgaaggagtg atttttttga aaataggata cgcccgggta tctactggtt
      961 tacaaaattt ggatttacaa gaagacagcc taaataaatt tggttgtgaa aaaattttta
     1021 ctgatcatat gagtggggca aaaagtaatc gaccaggatt agaaatggcc attgattttg
     1081 ttcgctctgg cgatacactt gtcgtttggc gcttagatcg tttaggtaga aatatggaag
     1141 atttgatttc aatcgtcaat cgactgaatg agcgtggtgt gagttttcat agtattcaag
     1201 aaaatattac gatggacaaa tcaagttcta caggacagct catgtttcat ttgtttgcgg
     1261 catttgcaga atttgagcgt aatttgatcc tggaacgatc cgcagcagga cgagcagctg
     1321 ctcgtgcaag aggacggttt ggtggtcgtc ctgaaaaatt gtctgaacaa gatttggaat
     1381 tactgaagac tttagtggat agcggtacac ctattaagac tattgcggaa cggtggaacg
     1441 tctcaagaac caccatttat cgttatttgg caaaaatgga ggagaaaaat ggaaagacaa
     1501 attctcatta gtttttgtca tttaatgctg aaaatttttg ccatttatct agaaaagtga
     1561 caatagtatg agaatttttt gtaagtgtct actgataact tattctcaaa tgttgtaagg
     1621 attttattca tttgttaata ttaa
//
LOCUS       DAZUKC010000192         1615 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000192 DAZUKC010000000
VERSION     DAZUKC010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.200"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(482..1234)
                     /locus_tag="ACG80T_002820"
     CDS             complement(482..1234)
                     /locus_tag="ACG80T_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4842412.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(1249..>1615)
                     /locus_tag="ACG80T_002821"
     CDS             complement(1249..>1615)
                     /locus_tag="ACG80T_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFS4842413.1"
                     /translation="ICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQDREL
                     SLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKKNPNM
                     FVNYQNDPYQFTTQVILLF"
BASE COUNT          530 a          283 c          211 g          591 t
ORIGIN      
        1 tagttggtgc tgcaggagga cctatttcgg ccgttacagg aggtattggt aatgtacttg
       61 tcggatgtgc attaaagcct gttcctgcaa gttaaattta taaaaacagg aaagtaatta
      121 tctcaaatcc tatcaagtac aatcatagga ttgcacttga caggacaaat tttttaacta
      181 aaaaatgaaa aacactttct aaaaattagt agaatttgcc aaatctttcg catcttccac
      241 catacattta attttaattc ccctttatgg tacttaataa aaacaagaaa agaactattc
      301 atttggccct tttttggttc cagaccatag aaatattcct ccagtaacta atattgctgt
      361 tatgcacgta actaataaca gctataaaaa ttggtacaac tttaatagga ttcagaaaaa
      421 tttgcactag tgaaggaatc tattttatta gaaaaatgca atctccctaa agaaaaaacc
      481 actaaacagt gctttctttc aacttctcct taattttttg tgctttccct aaagtaaagt
      541 ttctggccaa ctctgaatca aaatatagtg aacgtttttt aaaatctatt tctttaacgt
      601 taaagatatt tactaaacac gctcgactaa ttcttgttaa cataggatat ttcttttcaa
      661 gctcactaat tcttccataa aattcgtact gcccatcttt agtacacaat gataatctat
      721 gtggtaattt tgaagcttct acaaaataaa tgtcatttat atcaaaagta aatgtttgag
      781 aacctatgga aaaaacaaat gcttgattcg taacctgttt tgttgcatct atcctttctt
      841 gcgctaataa caataactca acaatctcgt ttcgatactc atctagtgtt tgatcttttg
      901 ttacaaatcc taacgtttca actcttcttt ttattgttac gggtaacatc tcatcatgag
      961 tagttgtaaa aatgattttt gcttgaacat catattttct gattacttct gctaattcta
     1021 taccgttaac ttcatgattt aaatcaatat ctaagaaata tattccttgc tttggtcgga
     1081 attgtttgag gtatttttta acttctaacg gactttgtgt tttcaatact atcttaaata
     1141 ctttatcgtg aaaaagaatg aaattatcaa taattcgttc taactgattt aattgtataa
     1201 tttgatcttc acataatatt attggataac tcataaatcc tccttccctc aaaacaacaa
     1261 aataacttga gtagtaaatt gatacggatc gttttgatag ttcacaaaca tattgggatt
     1321 ctttttattg atttcctcta tactagataa acctaaccct tgatgtccct tttttgttgt
     1381 tattcctttt ttcattagag tatctataga gagtagtatc tctctacaac tattcgatat
     1441 cgaaatttct atctgtttat catctcggta aatagctaga gacagttctc tatcttgact
     1501 ctctatagat gcttcaattg cattatcgag aataattcca agcaccctaa tacagtcaaa
     1561 tattggaaca ggaacttcaa taatagtact tggacattcg aatttacaac aaatc
//
LOCUS       DAZUKC010000193         1595 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000193 DAZUKC010000000
VERSION     DAZUKC010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.201"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..250
                     /locus_tag="ACG80T_002822"
     CDS             <1..250
                     /locus_tag="ACG80T_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842414.1"
                     /translation="TIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHDVDRN
                     EILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(612..851)
                     /locus_tag="ACG80T_002823"
     CDS             complement(612..851)
                     /locus_tag="ACG80T_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842415.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            1137..1451
                     /locus_tag="ACG80T_002824"
     CDS             1137..1451
                     /locus_tag="ACG80T_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842416.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          545 a          218 c          279 g          553 t
ORIGIN      
        1 aactatcatt ttaggaaaaa aatatatttg ccaacctatt ggcttgaaac aaaaagtgat
       61 tggagaagta atcagtaaat tagaaaattg tgttgtgatc tgcgtagacg aatatcatga
      121 cgtcgatcgc aatgaaatat tagaaaaatg cggaaaagta gtggcaaaat atactcacgt
      181 ttatgagttg gaaacaacag aatatctata taactatagc aacaaatatg aacatacttt
      241 agtttcataa agatttaaat aaagtgcctt caaaagcgag aagaccaagc ttttgaaggc
      301 actttattta gttggcagtg attgttcatg atttttgatt tattcaaaag gttcgcctag
      361 agaatctgtc tctgtaacga ggcctggtgg agaaactcga taaaaaccta tactgccaga
      421 actgtcctat tttcggatag acaaactagt agttttaaaa agatcatccg atccaatcat
      481 ttcaaaaaat gcaaaactga tatcaggatc atttagctca ggcttgttat cgtaaagcat
      541 ttgtaaaggt tgcatttagt taggagtaaa cgcgaaacta tcatcattat ttgctgaaga
      601 agatgaagaa gctaattcag agtttgtttg aaataaagga gacgtgctga cagtttcttt
      661 accctgtttt ttgcagaggt agaaactttt atatcggaag tttctaccgg agaatttttc
      721 tcatttgtag aaattgttga atcgatacta ttcgaaattt gcgtatctga aggtatgttc
      781 tttgatgcag aagtcccaca agcagtaagt aacaataaat ttgataaact gataaacaga
      841 attcttttca ttatgtacac actccttata gtatcgttta gcatacacct atgctcagta
      901 ttaggcaaaa tcaatgcaaa actatcaagt cttttatagt atctattagt cttagtaata
      961 catatatgga gagggtgata tcccagttca aaccagatac attggttacc atctgtatta
     1021 acgattatgc ctctaaacgc tttttttatg aaaaaattta taagtgaaaa cgtaggtatg
     1081 atattatcta gaggtaattt ttttgggtat ttaaatagtt gctaggagga tagcttttgg
     1141 atttttacag taattttatc ttaataatag caatattatt acttctaaat atttggtttt
     1201 ttgataagag tagaaatgca gggataggtt ttagaaccaa acgaagtacg tcttctgaaa
     1261 aaaagtgggt atattcacag actatttttt acggaggggt tatttcaatc agtttacttt
     1321 cttcaacact ttattctttt aatgttatag acgtttccat gtcaaatttt atatctatta
     1381 ttggaatcct tatttctgca attattacac aactacttct tgtgtttgaa gagaagtcaa
     1441 aaaataatta aagcaattat gccttttagt acaggaatta ttacttctta caaatgtata
     1501 gcgaatgtga agcctgctgt tttaattgtt ctagaaaaac ttttgagtga acaattattt
     1561 tatgttagga tgaattaatt tctctagagt ttata
//
LOCUS       DAZUKC010000194         1573 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000194 DAZUKC010000000
VERSION     DAZUKC010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.202"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            40..897
                     /locus_tag="ACG80T_002825"
     CDS             40..897
                     /locus_tag="ACG80T_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFS4842417.1"
                     /translation="MEEKLIKKDEIIMGLLVPRANTYTIPLTKRQLIAKRYQRITRAI
                     NREFWNSESDTAHSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQ
                     VIKNAFQEILPNSDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMG
                     GNWKATNPKKEQEAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEA
                     MGNWKFVENNSGGQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICL
                     EKVLKKIAL"
     gene            917..1477
                     /locus_tag="ACG80T_002826"
     CDS             917..1477
                     /locus_tag="ACG80T_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HFS4842418.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
BASE COUNT          603 a          198 c          282 g          490 t
ORIGIN      
        1 ataaattgct tgaagtatta tatttagata tcataattta tggaagaaaa attaattaaa
       61 aaggatgaga ttataatggg gttactggtt ccgagagcta atacttatac tataccttta
      121 acgaaacgcc aattaattgc taaaagatat caaaggatta ctagagccat caatagagag
      181 ttttggaatt ctgaaagtga tactgctcac agtttatacg taggttctta tgggagagga
      241 acggcaatta gtactagtga tatagatata atagttgaat taccgatggc agaatttgac
      301 agattcaaaa attatttatc aaatggcccg tctaagttat tacaagttat aaaaaacgca
      361 tttcaggaaa tactaccaaa tagtgatatt agagctgatg gacaagttgt taaaataaat
      421 ttccatgatg gaatcaaatt tgaaatagtt ccagctttta atgaaaaaga ttattggggt
      481 gagtcaaaag ggtttattta tccagattca aacatgggag ggaactggaa agctacaaat
      541 cccaaaaaag agcaagaagc catgaaatta aagaatacaa aaagtaataa tttattatat
      601 gctacgtgta aacattttcg acatgtacga gatacagaat ttacgagtta tcatctttca
      661 ggaatagtaa ttgacagttt cgtatatgag gctatgggaa attggaaatt cgttgaaaat
      721 aacagtgggg gtcaaaacat tagttctgta tcttatgaga cagctttatt agaatactat
      781 aactcacata aagttatggg cggtttaaat ttatactctc ctggaagcaa tcaatttgtt
      841 aattctgata gtagtatcat atgtttagaa aaagtattaa aaaaaatagc tttataaagt
      901 tttatggagg aacaacatgg gagacgaaaa aaagcgcttg gaagttcaat tacgtgaagc
      961 atatggacga gtaacgtata cttacaccac tcatttaaag caagccgata atattatttc
     1021 tgacaataaa attttaaggt gtcttcaaat tggtttgtca gcccttacta ctggaggggt
     1081 acttagtacc attattactg ataatagtga gttaaccttt gttgctggat tatgttcaac
     1141 aatttctctt ggattaaact tatactttaa gaactttgat cttgctgaag aagctaaaaa
     1201 tcatcaaatt gttgccgata aattatggtt gatcagagaa gaatacgtaa gcttactaac
     1261 agatttaaat cgtttaagtg ttgaagatat tgtgaaaaaa agagatcaat taatgaacga
     1321 aactcatgaa gcatataaaa atgcccctaa aacaaatagt aaaagctatg aatcagctca
     1381 gaaagcatta aaatctgaag aagagcaatt ttttacagaa gaggaattgg ataagatgtt
     1441 accagagcat cttagaaaaa agagttctaa atattgaact ctttttttat gcttaaaatt
     1501 ctcttgtaat ttttctattc aatcaataaa taatctatat ttaagaagaa atatgatgat
     1561 agaaacatct cct
//
LOCUS       DAZUKC010000195         1549 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000195 DAZUKC010000000
VERSION     DAZUKC010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.203"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            26..>1549
                     /locus_tag="ACG80T_002827"
     CDS             26..>1549
                     /locus_tag="ACG80T_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS4842419.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     "
BASE COUNT          601 a          213 c          273 g          462 t
ORIGIN      
        1 aagctgtatt caggaggtga agataatggg catcgaattt agcgaacggc aaatgaaaat
       61 actatacttt ttgattgaaa atgagcacac aacttacaaa gaattatcag aacaattata
      121 tgtatctacg aaaacaatta gcaaagatat tttaattttg aaagaaataa tccaaaaaat
      181 gccagatgta caaatgttgg ttcaacctaa aaaaggcgtt tcgcttattg gaaaaaaaga
      241 aacacttcga aatttattat ccagtcataa aatcaatatc gcagactctc cggaggaccg
      301 tctaacttat gtgtttgcta aattattgaa tactaatgat tctctaaaga ttcaaaatct
      361 tgctgacgaa ttatacgtga gcaggtcgac ggtagaaagc acaatgaaag atgttagaaa
      421 aagactaaac aagcatggga tgtccttagt ttcagatcga aatggcgtgc gcatcaaagc
      481 tgatgaaaaa agtaaaagaa ggttgatgtc agaagttgtg aactattatt ggggtggctt
      541 tacagcatct aacagtaaaa cagatctctt agacttgaat atcaatttca ttggtgaagc
      601 gaataatttc ttaaataagg acatattgaa aaaagtagcc gaattgctta atcgatacat
      661 gaataagaca caattgaaat ttacagatta tgaatttcaa actttagcta tccaccttgc
      721 tattgcgata gaaaggattg aaacaagcaa tttcttatca cgtagtaata attctgaaga
      781 agaaatctca aagaatacac ttattttaat caaaatgatt gaaaaagcat tttcaattat
      841 tgtgccaata gatgaacaag cttatgttga cattcacatt acttcgattg aaaaaaatgc
      901 tatgaatcac gacttagatc aaacgaagct ttcctattta gatatgggtg ttgagctaca
      961 agagttagta agaattaaat taaatgaatt ttatcctgat gaagaattga tcaataatct
     1021 agtattacat ctcaatgcag cagttaaaag actcaaattg ggagtaaata tctataaccc
     1081 ttatacggac aaaatcaaat attcttttaa aaggtcattt atgataagtg tagatttatt
     1141 agaggaaatc gaagagagat tctgtataca ttttaatgaa gatgaaatcg cttacgtaac
     1201 actccatatt caatcacttt tggatcgtta caaacctgac aaaacgaaag tgatattagt
     1261 ttgtagtagc ggatatggaa catcaaaatt attagagcaa agaataaata acggttttgc
     1321 agatatggtt gagatagaag atgtactaag catcaatgaa ctacaagata gaaaagtcac
     1381 tgatgaactt gttataagca cgattccgat tgaaaataca aattttcctg ttattgttgt
     1441 tagtcctttg atgttagggg cagatgtcag aaaagtaaaa cagtatctac agttaaatga
     1501 acaaacagaa gatgcttttc taaatttatt agataaacga ttttgcttt
//
LOCUS       DAZUKC010000196         1536 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000196 DAZUKC010000000
VERSION     DAZUKC010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.204"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>1536)
                     /locus_tag="ACG80T_002828"
     rRNA            complement(<1..>1536)
                     /locus_tag="ACG80T_002828"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          321 a          476 c          337 g          402 t
ORIGIN      
        1 gggaaatctc atcttgaggt gggcttcaca cttagatgct ttcagcgttt atcccttccc
       61 tacatagcta cccagcaatg cccttggcag aacaactggt acaccagcgg taagtccatc
      121 ccggtcctct cgtactaagg acagctcctc tcaaatttcc aacgcccgcg acggataggg
      181 accgaactgt ctcacgacgt tctgaaccca gctcgcgtgc cgctttaatg ggcgaacagc
      241 ccaacccttg ggaccgacta cagccccagg atgcgacgag ccgacatcga ggtgccaaac
      301 ctccccgtcg atgtggactc ttgggggaga taagcctgtt atccccaggg tagcttttat
      361 ccgttgagcg atggcccttc catgcggaac caccggatca ctaagcccga ctttcgtccc
      421 tgctcgactt gttggtctcg cagtcaagct cccttctgcc tttacactct tcgaatgatt
      481 tccaaccatt ctgagggaac ctttgggcgc ctccgttacc ttttaggagg cgaccgcccc
      541 agtcaaactg cccatctgac actgtctccc accacgatta gtggtgcggg ttagagtggc
      601 cataacgcag gggtagtatc ccaccagcgc ctccatcgaa actagcgttc cgatttctac
      661 ggctcctacc tatcctgtac atgcggtaca gacactcaat atcaaactac agtaaagctc
      721 catggggtct ttccgtcctg tcgcgggtaa cctgcatctt cacaggtact aaaatttcac
      781 cgagtctctc gttgagacag tgcccaaatc gttacgcctt tcgtgcgggt cggaacttac
      841 ccgacaagga atttcgctac cttaggaccg ttatagttac ggccgccgtt tactggggct
      901 tcaattcgta ccttcgcttg cgctaagcac tcctcttaac cttccagcac cgggcaggcg
      961 tcagccccta tacttcatct tacgattttg cagagacctg tgtttttgat aaacagtcgc
     1021 ttgggcctat tcactgcggc tgatcgtatg atcagcaccc cttctcccga agttacgggg
     1081 tcattttgcc gagttcctta acgagagttc tctcgctcac cttaggattc tctcctcgac
     1141 tacctgtgtc ggtttgcggt acgggccgct gttttctcac tagaagcttt tctcggcagt
     1201 gtgacatcag gaacttcggt actattattt ccctccccat cacagcttgt ccttaagaaa
     1261 agaagcattt gactcctctt cagacttact gcttggacag acatttccga tcgtctgcat
     1321 tccttagcct cctgcgtccc tccattgctc aaacaaaaac aacgggtaca ggaatatcaa
     1381 cctgttatcc atcgcctacg cctgtcggcc tcggcttagg tcccgactaa ccctgggcgg
     1441 acgagccttc cccaggaaac cttagtcatt cggtggacag gattctcacc tgtctttcgc
     1501 tactcatacc ggcattctca cttctaagcg ctccag
//
LOCUS       DAZUKC010000197         1498 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000197 DAZUKC010000000
VERSION     DAZUKC010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.206"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..340)
                     /locus_tag="ACG80T_002829"
     CDS             complement(<1..340)
                     /locus_tag="ACG80T_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4842420.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEERE"
     gene            complement(337..990)
                     /locus_tag="ACG80T_002830"
     CDS             complement(337..990)
                     /locus_tag="ACG80T_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4842421.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1278..>1498
                     /locus_tag="ACG80T_002831"
     CDS             1278..>1498
                     /locus_tag="ACG80T_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS4842422.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEK"
BASE COUNT          481 a          257 c          161 g          599 t
ORIGIN      
        1 tttctctttc ttcaattatc aaagaattac tatcactttc taaattaaca tttatattat
       61 ctttgatttg tatttcattc ttattatttt tttcttttat gaatactttt atttcactac
      121 tcttggaata aaactccaaa gtttgttcta tatattttat ttcttttcca ttcatattac
      181 tagaaatttc atttgccata ttatgaatct tttcttttct cgtctccaaa tatgttttag
      241 gaaagataaa aaaaattaat gaatgaacta atataattat tattccaaga acagaaaata
      301 tttgtatgaa catttttgga aatatcttta attttttcat tttctctcca atttatatcc
      361 tacatttctt atagtagtta tacagtctaa ttgtaatttt tttctaagtt cttttatata
      421 tacatctatt actcgatcat aaggagtttc ttcgctatct ttccatacat aatcaataat
      481 ttgcattctt gttaacactt gtccattatt atccaataaa cattttaata cttctagttc
      541 ttttgcattt acatctattt cttcaccatt tattttagct gtatagctat taaaattaac
      601 cgatagatcc ttatattcaa acttctctaa atgtccataa ttcttcttaa ttaaagaatc
      661 tattctagct tttaagaccg gcaatgaaaa tggcttttct acatatccgt ctactaaatt
      721 agtaaatgca tcaattttat attcttcatc actaaatgca gtcaagatta gaattggtaa
      781 attgcttttc tttctaattt ctttcaacac ttctaaacca tttataaaag gtatctgaat
      841 gtctaagata accaaattta tatcgctatt aaattttgac aaggcttctc ttccatcttt
      901 agcttgaata acagtatatc caaattctga aagatattca cttattccct ctcttatcat
      961 attatcatct tcaacagtta atattttcat atatacctcc acttaagcct atacactaga
     1021 tattatacag catattatgg ctctataata tctagtgttg gtgacacctt ctgttgccaa
     1081 tactttttct tttttctaat aaatgcaaac agacaccttg aaatccttta gaataaaggg
     1141 ttctataata ttttgtgttg ttggaaaatc gaaggcgatt ttctactaac actttttctt
     1201 cgtttcaact caaaaagaga ctcacttttg gtaaaattta gtcgaccaaa acatcaccct
     1261 accaaaggag tcgctctatg gataatcata ctagaaaatt actcggatta acagataaac
     1321 atttattttt tgatgaagaa tggctgattg aaaaagaata taaaggcgtt caagcgcagt
     1381 ttattaaagg gagattggat gactcacctt cccattgtga acactgtgga tcgattcgta
     1441 ttatccgcaa cggttcctat acgacacgta ctcagatatt gaaagtcaaa gaaaagtt
//
LOCUS       DAZUKC010000198         1459 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000198 DAZUKC010000000
VERSION     DAZUKC010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.207"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..110)
                     /locus_tag="ACG80T_002832"
     tRNA            complement(26..110)
                     /locus_tag="ACG80T_002832"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(74..76),aa:Leu,seq:caa)
     gene            complement(128..199)
                     /locus_tag="ACG80T_002833"
     tRNA            complement(128..199)
                     /locus_tag="ACG80T_002833"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(165..167),aa:Gln,seq:ttg)
     gene            complement(209..281)
                     /locus_tag="ACG80T_002834"
     tRNA            complement(209..281)
                     /locus_tag="ACG80T_002834"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:His,seq:gtg)
     gene            complement(343..427)
                     /locus_tag="ACG80T_002835"
     tRNA            complement(343..427)
                     /locus_tag="ACG80T_002835"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(391..393),aa:Leu,seq:caa)
     gene            complement(448..518)
                     /locus_tag="ACG80T_002836"
     tRNA            complement(448..518)
                     /locus_tag="ACG80T_002836"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(484..486),aa:Cys,seq:gca)
     gene            complement(564..635)
                     /locus_tag="ACG80T_002837"
     tRNA            complement(564..635)
                     /locus_tag="ACG80T_002837"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(601..603),aa:Gln,seq:ttg)
     gene            complement(647..719)
                     /locus_tag="ACG80T_002838"
     tRNA            complement(647..719)
                     /locus_tag="ACG80T_002838"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(684..686),aa:His,seq:gtg)
     gene            complement(722..795)
                     /locus_tag="ACG80T_002839"
     tRNA            complement(722..795)
                     /locus_tag="ACG80T_002839"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(761..763),aa:Trp,seq:cca)
     gene            complement(802..882)
                     /locus_tag="ACG80T_002840"
     tRNA            complement(802..882)
                     /locus_tag="ACG80T_002840"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(846..848),aa:Tyr,seq:gta)
     gene            complement(884..959)
                     /locus_tag="ACG80T_002841"
     tRNA            complement(884..959)
                     /locus_tag="ACG80T_002841"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(924..926),aa:Phe,seq:gaa)
     gene            complement(995..1067)
                     /locus_tag="ACG80T_002842"
     tRNA            complement(995..1067)
                     /locus_tag="ACG80T_002842"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1032..1034),aa:Asp,seq:gtc)
     gene            complement(1077..1149)
                     /locus_tag="ACG80T_002843"
     tRNA            complement(1077..1149)
                     /locus_tag="ACG80T_002843"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1114..1116),aa:Val,seq:tac)
     gene            complement(1165..1236)
                     /locus_tag="ACG80T_002844"
     tRNA            complement(1165..1236)
                     /locus_tag="ACG80T_002844"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1201..1203),aa:Glu,seq:ttc)
     gene            complement(1251..1339)
                     /locus_tag="ACG80T_002845"
     tRNA            complement(1251..1339)
                     /locus_tag="ACG80T_002845"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1303..1305),aa:Ser,seq:gga)
     gene            complement(1379..1452)
                     /locus_tag="ACG80T_002846"
     tRNA            complement(1379..1452)
                     /locus_tag="ACG80T_002846"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1416..1418),aa:Asn,seq:gtt)
BASE COUNT          415 a          385 c          334 g          325 t
ORIGIN      
        1 aagaaagttg ctatattgat aattataccg gcggccgggg tcgaaccggc acgtcctcaa
       61 cggacactgg attttgagtc cagcgcgtct gccaattccg ccacgccggc aaatttaaaa
      121 ataataactg gggtagctgg attcgaacca acgcatgagg gagtcaaagt cccttgcctt
      181 accgcttggc tataccccaa gaataaaggg cgaatgatgg gaatcgaacc cacgaatgcc
      241 ggagccacaa tccggtgcgt taaccacttc gccacattcg ccatcataaa agatgttatt
      301 taatttctca ataaaaaact aagatgaaga catcttagtc aataccggcg gccggggtcg
      361 aaccggcacg ccctcaacgg gcactggatt ttgagtccag cgcgtctgcc aattccgcca
      421 cgccggcaag ttgatttatt agtactaagg cggtaaccgg atttgaaccg gtgatgaagg
      481 ttttgcagac ctctgcctta ccacttggct ataccgccat tatactatta tcaatctaca
      541 aaataaagaa taaattatag aaactggggt agctggattc gaaccaacgc atgagggagt
      601 caaagtccct tgccttaccg cttggctata ccccaagaat aaaaagggcg aatgatggga
      661 atcgaaccca cgaatgccgg agccacaatc cggtgcgtta accacttcgc cacattcgcc
      721 atggcagggg cagcaggaat tgaacccaca ctaacggttt tggagaccgc tgttctacct
      781 ttaaactatg cccctataaa atggaggaga gtggattcga accactgaac cctaaggaac
      841 ggatttacag tccgtcgcgt ttagccactt cgctactcct ccatggtggc gcgggacaga
      901 atcgaactgc cgacacacgg agcttcaatc cgttgctcta ccaactgagc taccgcgcca
      961 tattttaaaa ttattaattg aataacaaga acgacggttc cgacgggaat cgaacccgcg
     1021 atctcctgcg tgacaggcag gcatgttaac ccctacacca cggaaccagt tcattatgga
     1081 ggttaacggg atcgaaccgc tgaccctctg cttgtaaggc agatgctctc ccagctgagc
     1141 taaacctcca acattcaatt aaaatgaccc gtacgggatt cgaacccgtg ttaccgccgt
     1201 gaaagggcgg tgtcttaacc gcttgaccaa cgggccaggt aaatttgcta cggagagtaa
     1261 gggattcgaa cccttgagac agtttgcacc atctacatga tttccaatca tgctccttcg
     1321 gcctctcgga caactctcca gagtgaaaga agctcaatac tctttctcaa aatgagaact
     1381 ccggcagtag gactcgaacc tacgacatca tgattaacag tcatgcgcta ctaccaactg
     1441 agctatgccg gaataacat
//
LOCUS       DAZUKC010000199         1459 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000199 DAZUKC010000000
VERSION     DAZUKC010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.208"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(15..1154)
                     /locus_tag="ACG80T_002847"
     CDS             complement(15..1154)
                     /locus_tag="ACG80T_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFS4842423.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT          382 a          316 c          263 g          498 t
ORIGIN      
        1 ttattaaaaa aatcttacgc taagtaggat aaaataattc cttgatattt ttcgatcata
       61 tccaaataat tatcgatttc aacatactca tctacttgat gaggcaaagt aacgacacca
      121 ggcccaaaga cgacaaagtc gaaggaatgg ttagctttcg taaattcggc tgcatccgtt
      181 gttgctgccg ctccaactaa aggtaatggc tggctgaatt gttgctgaat gcaatgaatc
      241 aaaggagaat ctggatcagc tttgacaggt attttgttgt aatcgatcat caattccaaa
      301 tgatagtctg tctcttgatt caattcattg acgattgatt gcagcaatgc aatgatttta
      361 tcatttgggt attctggaat cgatcgaatg ttcccttgga gctgcgcatg gcttgggatg
      421 ctgttgactt gattcccgcc actgatcaat gtaacattat ggattgtttt tcctaggaca
      481 ggattctcga ttgtttcagc gaggtgattc atttcagcat ttgctttcgt aatgaattca
      541 tttaggtgat tgattgcatt ataaccttgg tctggcatag agctatgcgc ttcttttccg
      601 tgcgaggtga ctgtatagtt gattgatccc atgtgcgtat acatcaaact gtaattggtt
      661 ggctctccga tgatcaaggc atctaagtca tctacatagc ctgctttcgt gagctgttcc
      721 ccacctagtt cacccacttc ttctccgaca gtggctaata atttgactgt tccgttaaat
      781 ggttttcctg actctttcag ttcgatcatg gcaatcacca ttgctgccaa gccactcttc
      841 atatctgtag caccacgtcc gtacaaacgg ttcccatgga tttctgctgc aaagggtgca
      901 taggtccaag acgattcatc accagccgcc acgacatcca tatgcccaga taggcctaac
      961 acttttcctg attggccctt ttggtaacga gcaatcaaat tatctctacc atctctgtaa
     1021 gaaacaatct ctcctgttat gtcatgcttt gccaaaagct tattcaaata ggctgctact
     1081 tcaccttcgt ttccattcac tgatttaatt cggataatct cttgtaaaat cgcgattttt
     1141 tcttcttttt tcatctaatc ctctcccttg ccatttcttt ttattgtatt tgagtgtgct
     1201 cgaattttca aataggagca accaaagtaa aaatgaagaa agagagccac tttatgaaaa
     1261 atatgctgct acttccctcc tattttattt atacgagagg acttgcccat agattcctat
     1321 tctactgttc tatccttaaa agctcttata tgatgctagt tattgttagc tttccttttt
     1381 caaggatgat cttttcttgt accattcttt gtaaacatca tatacagaag ctagttgcaa
     1441 aaaaactctc tacctgctg
//
LOCUS       DAZUKC010000200         1444 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000200 DAZUKC010000000
VERSION     DAZUKC010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.209"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..825
                     /locus_tag="ACG80T_002848"
     CDS             64..825
                     /locus_tag="ACG80T_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFS4842424.1"
                     /translation="MHTPEEIMELSIHLGTKKIHKPILAKLILGFIGGAMISLGYLAY
                     VRVSASIPESMASLTSLVGASVFPIGLLVILLGGGELITGNMMAVAIAFMDKKVTFSQ
                     LVKNWSVITFANIIGAIFVAYFFGHFVGLTHSGVYLDQVIHIADHKIAVSWWQALGSG
                     IGCNWFVGLALWLSYGAKDAAGKVLTIWFPVMIFVAIGFQHSVANCFVIPAAIFEGQA
                     TWLEFLSNFIPVYIGNIIGGAIFVSGFYYVSYKHH"
     gene            1053..1247
                     /locus_tag="ACG80T_002849"
     CDS             1053..1247
                     /locus_tag="ACG80T_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842425.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          464 a          221 c          272 g          487 t
ORIGIN      
        1 gtgatttttt tcactatatt ttatcacatg aaaattcatt aagaaagtag gaatcgaagt
       61 attatgcaca caccagaaga aattatggaa ctcagtattc atcttggaac aaaaaaaata
      121 cataaaccta tcttagctaa actgatttta ggttttatcg gaggagccat gatttctcta
      181 ggttatttgg catacgtacg agtctctgct tctatcccag aatctatggc tagcttgact
      241 agtttagttg gtgcctcggt ttttcctatc ggcctgctgg tcatccttct tggcggtggt
      301 gaattgatca ccggaaatat gatggctgtg gcgattgcct ttatggataa aaaagtcaca
      361 ttctcacagc tggttaagaa ttggtcagtg attacttttg caaatattat tggtgcaatt
      421 ttcgttgcct atttctttgg acactttgtt ggtttaactc attcaggtgt ctatctcgac
      481 caagtgattc atattgctga tcataaaatc gctgtttctt ggtggcaagc attaggctcc
      541 ggaataggtt gtaattggtt tgtcggttta gcgttatggt tatcatatgg agcaaaagat
      601 gcggccggca aagtactaac gatttggttt ccagtcatga tttttgtggc aatcggtttc
      661 cagcatagtg tggccaactg ttttgtgata cctgctgcca tatttgaagg tcaagcgact
      721 tggttagaat ttctctcgaa ttttatcccc gtttatattg gaaatatcat cggcggagct
      781 atttttgttt caggttttta ttacgttagt tataagcatc attaagattg aaggtaatgt
      841 tcattcgagc attaccttca atctctttat atactattta tattttaaga acaatagcct
      901 tttattatat atattattca aattagttat atcgagtgaa aaaaactttc aaattgattc
      961 tttttattta tcataaagat atagatacat aatcattttc acttacatat atttgaatat
     1021 taactaaaaa cagcaagaaa gaagaggatc aaatgtatta tgtagaagta aaaacaaaag
     1081 gcgtcaaaaa caaacaatat gtgaaaggta tcagtaacga atatccttta cttggttcat
     1141 ggaaagaagc tgctcctttt tccaaaccat gtgccataaa aataaagaac gagctagaaa
     1201 aagaactgac ttgtggcaaa gctgttgtag atatcatcga aaagtaaatc caatgtatag
     1261 aaaggaagat cacacatgaa aagcgacgta caaattgcta agattataga attagaagaa
     1321 gaagacgcag tgttgacaat tgaaaaaaaa taaatgtttg tataccatat gattatttca
     1381 atatgtatcc tggaattgga gatctcgtag agctatatca agatgaatca aatatacttg
     1441 tcat
//
LOCUS       DAZUKC010000201         1442 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000201 DAZUKC010000000
VERSION     DAZUKC010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.210"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            73..234
                     /locus_tag="ACG80T_002850"
     CDS             73..234
                     /locus_tag="ACG80T_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFS4842426.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            253..>1442
                     /locus_tag="ACG80T_002851"
     CDS             253..>1442
                     /locus_tag="ACG80T_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4842427.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLI"
BASE COUNT          587 a          131 c          243 g          481 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaactaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcactcg aagctacaaa taatactaaa agaaatattt tatttagtag tggagtattt
      661 acaattaaag aaggtaaaaa tatagaagaa aatgataaga attcaattat tgttcatgaa
      721 gaatttgcta aacaaaacaa tctaaaatta ggtgatgaag ttgatcttga attactagat
      781 attgaaaaaa gtggaaaaat aaaaagtcat aaatttaaaa ttatagggat cttttctggt
      841 aaaaaacagg aaacatatac aggattatct tctgatttta gtgaaaatat ggtgtttgta
      901 gattattcaa caagccaaga gatattaaat aattcagaaa ataatgaaat tgcaaataaa
      961 attttaatgt attctggtag tgcagaatct acagacttag ccttaaacaa attgaaagag
     1021 cttaaaattg atgagtcaaa gtattttgtt gaaaaagatt ctaatgcgtt tgaagagtct
     1081 ttagagtcag tgagtggaat aaaatatata ataaaagtaa tgacttattc tattatgtta
     1141 ggtgggatgg ttgttctttc attaatcttg attctatggt taagggaaag aatttatgaa
     1201 ataggtatat tcttatctat tggaacatct aagatacaaa ttataatgca atttatattc
     1261 gagttaatat tcatatcgat accaagtata atatcttcct tatttttagg aaatgtatta
     1321 ctaaaagtaa ttgtagatgg attcattaac tcagaggatt caatgatttc cggtggaagt
     1381 ttaataaata atagtagttt tatgtcaaat ataacaacac ttggacaaag ttatttaata
     1441 tt
//
LOCUS       DAZUKC010000202         1433 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000202 DAZUKC010000000
VERSION     DAZUKC010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.211"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..944)
                     /locus_tag="ACG80T_002852"
     CDS             complement(<1..944)
                     /locus_tag="ACG80T_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFS4842428.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERPVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAF"
BASE COUNT          455 a          246 c          254 g          478 t
ORIGIN      
        1 taaaatgctt cttgtacata gctcaaaaat atttcgattg gtgttctgta attcaatgat
       61 tttcttggaa tatgattacg ttgattgctg acactggaaa taaatgtctg attcactttt
      121 ctaaaatcca ttgatttcgg cagtccatta cgacgcagaa tcccgttaga attctcgttt
      181 aatgggcgtt gagaaggtgt tccagggtcc gcaaaatata tatcaatatc atgttggtta
      241 ctaatggctt tccagttaga aaattctttt ccacagtcaa acgtaataga tttaaagaag
      301 tttttaggga agcgagaaaa ccattgatta agggcagttt caatatctaa tgccttacgg
      361 ccgttgggtt taatcgtgat aatgacttta gataatcttt caactaaagt aatgacggca
      421 cttttatgat gaatgccaac gatcgtatca ccttcaaggt gaccaaactc agaattgaaa
      481 tcaggataat tatcaggtcg atcatggatt gagcgctgat actgttgttt tccccgtttt
      541 tcctgatggc cattgggttt tcttttacct ttcatcggta gtgtgtcaat atcaaatatt
      601 cctttagaaa ataaacgata aagtgttctc ataccgcatg aaacaggcct ttcttttcgc
      661 ccgataatga cgtcaggcgt ccaaccaaga gtgactttct ctttaatgta atctacttca
      721 tgagcaggta attgaatgac ttttctacca cattttttct tattttcttt atactggtgc
      781 caataatcaa gagcagtctt gccttgcttg aacttattga ctacattata aatagtttgt
      841 atagcacgtc ccattcggtt agcgatttca accggcttat tatgttgaag ataatatgat
      901 tctatcattg tcagttcgtc tatggtaaga tgtttgtagg tcatttatgg ttacactcct
      961 ttgttttctt tcgtcggaaa tacaatttga gtgtaccata aatgcctttt tatttttcta
     1021 acttaatttt acaattcgcg atatattaaa caacgttgtc gcgcttcatt gaaacatttt
     1081 atgaaaattc caaaaaagag ttgacacatg tttttagacg tgataagata actaagtcgt
     1141 taaaaagaac cacttgcgaa gcttgattct atcgaaaaaa aacttcaaaa aagttcttga
     1201 caaataacaa acgataagtt atgataaatg agttgctgaa agatttcgaa aaacgaaatc
     1261 gaaaaaaact tttaaaaaag agttgacaat cgcttgtctc tctgatatga tataaaagtt
     1321 gctacggcag ccaaaaacga atcgggaaac actcccgatg aagtagatct ttgaaaactg
     1381 aacaaagtaa gacaaaccaa atgtgtaggg cgtcttgatt caattcaaga caa
//
LOCUS       DAZUKC010000203         1424 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000203 DAZUKC010000000
VERSION     DAZUKC010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.212"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..1424
                     /locus_tag="ACG80T_002853"
                     /pseudo
     CDS             4..1424
                     /locus_tag="ACG80T_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          590 a          223 c          318 g          293 t
ORIGIN      
        1 gtcatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ttagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccagaga
      121 aagaccaacg gtcaaccaat gaacgataag agaaatgggc attcttggtt taggcaacaa
      181 gagcgaatcg aaaataaagt gcatagtctg agagaagaga tcaagcaaca agaaaaacaa
      241 gtagaaaaac ttgaaagaca agaagagata aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgagaa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcga ctttttcagc cgctacgatc cgaaaatacc aacggaaatt ggaaacatta
      421 gaacagctga aagaaagatc ggaaaaagga aaagaaaacc ttcttcctga agtacaagca
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaatcc gaccatttat tttctgaaag
      541 gctatcgaaa agtcgcttta gaactgaatg aaaacgggga atttgaggaa tctaaaaaat
      601 accaagccac gtcggaagag gaaaaagcag ctgttcgtga attacttgaa gaggctgtac
      661 caactgattt tttgaaggag aaagaaaacg atcattttta tcgagtagaa ttcaacgaaa
      721 atgatccaga aaaaggattg aaaagttatg gtggggagat cgtaacattc gaattgatcg
      781 atcaactcaa aacgttggat aaccaatacg ccgcagaagc aggttattat aaattctatt
      841 ttgaagaagt gaagaacgga gaagtagtcg atcagatccg aatggatcta ggggatggcg
      901 tcaacagtaa tcaacccatc tatttagagt tagcaaaatt gtgcgaacaa gtacctgaga
      961 aggaacaaaa acaatcggtc aaaaatgaac ctgtttcgaa agaacaaatg cagtcgaaag
     1021 aaacaaaaac aaaacaaaag ggctatcaaa aacgaacggc agaagagatt aaacaagaag
     1081 ttaaatccct ttcgcaacag gcactggatg cggtgaaaaa gcatactcat tctcctgaag
     1141 acgtcaaaga acttttgaac tttatgtctc gttttccaga acgatccttt agaaatcaat
     1201 tattgattga ggaacaattt ccaggtgcaa cggcttgttt aggtcgtgcc gcgttgaaaa
     1261 aagaaggaat ctatatcaaa aaaggggaac aagggaacaa gatctttgtc agaaaaacgg
     1321 tcaaaggatt ttatgagaaa ggacgtggct ttgttcgaga aatagaagct acaccagaag
     1381 ataaaaaacg aatcgaaagt ggtcaaatcg aagtgatcaa aaaa
//
LOCUS       DAZUKC010000204         1424 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000204 DAZUKC010000000
VERSION     DAZUKC010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.213"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..1337)
                     /locus_tag="ACG80T_002854"
     CDS             complement(150..1337)
                     /locus_tag="ACG80T_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFS4842429.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DAZUKC010000205         1398 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000205 DAZUKC010000000
VERSION     DAZUKC010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.214"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            120..1298
                     /locus_tag="ACG80T_002855"
     CDS             120..1298
                     /locus_tag="ACG80T_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFS4842430.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRG
                     VQEVLLFITDGLKGMKDTIHQIYPKAKYQHCCIHVSRNIAHKVRVKDRKEICDDFKAV
                     YQANSKEEANTFLSGMIEKWKKNYPKVTQSLIENQDLLTFYDFPPSIRRTIYSTNLIE
                     SFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLVSMFTE
                     "
BASE COUNT          520 a          250 c          230 g          398 t
ORIGIN      
        1 agaaagattt tcgagagtat aaaatatttt gtgtaaatga aaaaatccat acaaaaaagg
       61 aagtcccttc tgtagaataa agttaacgac aaccaattca cagaaaagag gacttcccta
      121 tgaatgattt tactacagaa attgtgcaaa ctctagtcac taaaggcgat ttaaatgaat
      181 tattccgttc gcacttagaa aaagcgataa acacactcct acggactgaa ttaacggctt
      241 ttttagatta cgaaaaatat gatcgcactg gttttaattc aggtaattcg agaaacggtt
      301 cttactttcg atcaatcaaa accgaatatg gtgaattaac attggaaata cctagagatc
      361 gtaatggtga gtttaaacaa caaactttac cagcctacaa aagaacaaac gatacattgg
      421 aaaccactat tatccattta ttcgaaaaag gtgttacgat gtctgaaatt gctgatttga
      481 tcgaaaaaat gtacggtcat cactatactc cacaaaccat gtccaacatg actaaagttc
      541 tgactgaaga agtaaatgcc tttaaatcca gagccttaaa tgataagtat gtcgctattt
      601 ttatggacgc tacttacatt ccactaaaac gtcaaaccgt atccaaagaa gcgatttata
      661 ttgccattgg tatacgagaa gacggcacta aagaagtact gagttatgcg attgctccaa
      721 ctgaatcaac atacgtttgg aatgagctgc tacaggatat taactccaga ggagttcaag
      781 aagtcttgct ttttattacg gacggcttaa aaggcatgaa agatactatc catcaaattt
      841 atcctaaagc aaaatatcag cattgttgta tccatgtatc tcgtaacatc gctcataaag
      901 tacgtgtcaa agaccgaaaa gaaatctgtg atgactttaa ggctgtttat caagctaact
      961 caaaagaaga agcgaatacc ttcttatccg gcatgattga gaaatggaag aaaaactatc
     1021 ctaaagtgac gcagtcactc atagaaaacc aagacttatt aactttttat gattttccac
     1081 ctagcattcg tagaaccatt tactcaacca atctaatcga gtctttcaat aagcaaatta
     1141 aaagatacag ccgtagaaaa gagcagtttc aaaatgaaga atcactagaa cgctttctag
     1201 tcagcatttt tgatacatac aatcaaaaat ttctaaacag aagccataaa ggttttcaac
     1261 aggtaaccga tacattagtt tcaatgttta ctgagtaact aattattttg caggaggaca
     1321 atttatttac acaaaattat tgacgctccc aattaggact caattgttca tagatgagca
     1381 tttgtaatta agttatat
//
LOCUS       DAZUKC010000206         1392 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000206 DAZUKC010000000
VERSION     DAZUKC010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.215"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            7..417
                     /locus_tag="ACG80T_002856"
     CDS             7..417
                     /locus_tag="ACG80T_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS4842431.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            431..787
                     /locus_tag="ACG80T_002857"
     CDS             431..787
                     /locus_tag="ACG80T_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS4842432.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          510 a          209 c          260 g          413 t
ORIGIN      
        1 gagaaaatga taaaagtata tacttcgcct agctgtactt cctgtagaaa agcaagagca
       61 tggttgcaag cgaatcaact tgaatttgaa gagaagaata tttttgccga accgttaaca
      121 gaaagtgaga tcaaaaagat cttggcagcg actgaaggag gagttcaaga tattatttcc
      181 actcggtcga aaatttacga aaagctgggc atagatttta acgaattaac tttaaagcaa
      241 atgatcgtct tattcaaaga atatccttca ttattgagac gacctatttt gattgatgaa
      301 aaaagaatac taatcggatt taatgaagat gagatccggt catttgtccc tcgtgaaatt
      361 cgatgtgttg ccagaaatct gatctattta caaaacaata acatcttaaa ttcataggga
      421 gggagccttc gtggaagtga aagtctatgg atcagcgcac tgtgtaacaa gtctgaaggc
      481 gaaacaatgg ttagaacacc atcaattgcc ttatcaattt gttgacttag aagaacgcga
      541 tctaagtaca gaagaagcag aagaattatg cagttttgaa gaagtgcaag cagaacagct
      601 atttgcgact tggtctgagg cttttcacaa cattgcgatt gacctttcct gtgtaggaaa
      661 aaaacaactg atttttttat gcacacaaca taccaatctc ctcagacgtc ctttgattat
      721 tatcgatgaa gttctattta ttggatacaa tgagcagttg atggaacagc ttatcttaaa
      781 taaatagact ttaactgttt taatcaagtc actgagcatt tcatacagtt gtaacagtcg
      841 aacacttagc atcaagcgtg ttatgtgttc tttttttgac tttatttttc tactatagag
      901 acatgctcaa tttatgtggt caaaattcaa atctaggtgg ggatatagag ttaaaaatat
      961 attgacatgt gtaaatttac atgatatatt catttctgtt gttttaagta gcaaagcaat
     1021 aaaatagtat tttaaaaaac atataaaata gttgttgaca tgttagaaat gacatgataa
     1081 gataactgag ttgttaaaaa agaaccactt gtgaagcttg attctatcga aaaaaacttc
     1141 aaaaaagttc ttgacaaata acaaatgata agttatgata aatgagttgc tgtaagattt
     1201 cgaaaaacga aatcaaaaaa acttttaaaa aagagttgac aattacttgt ctctctgata
     1261 tgatataaaa gttgctacgg cagccaaaaa cgaatcgggg aacactcctg atgaagtaga
     1321 cctttgaaaa ctgaacaaag taagacaaac caaatgtgta gggcgtcttg attcaattca
     1381 agacaacaaa ca
//
LOCUS       DAZUKC010000207         1364 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000207 DAZUKC010000000
VERSION     DAZUKC010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.216"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACG80T_002858"
     CDS             complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACG80T_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="HFS4842433.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          392 a          242 c          217 g          513 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcggatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgcc
//
LOCUS       DAZUKC010000208         1360 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000208 DAZUKC010000000
VERSION     DAZUKC010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.217"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1360
                     /locus_tag="ACG80T_002859"
     CDS             82..>1360
                     /locus_tag="ACG80T_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HFS4842434.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAY
                     PYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQ"
BASE COUNT          461 a          255 c          259 g          385 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgtca gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaaatt gtcaggtatc tttgaccacc gttattcgta cgttgaaaga gtttaaaagc
      541 tacttaccaa agcaatctaa gaggattctc cccagagtat tgatggttga tgaatttcgt
      601 tcgcacgctt ccatagaaga taaaatgagc tttatttgcg cagatggcga aacaggacaa
      661 ttaattgatg tcttgcctac gcgtaaattg cctcgattaa caagctattt cttaggctgc
      721 gctaatcctg aagaagtaaa atttttagtc acagacatga acgccgccta cttccagctt
      781 accaaacgta ttctgccaaa tgcgaaagta gtgattgatc ggtttcatat tatcaaacac
      841 atgaatcaag cctttaatga gttgcgtatc cgtgaaatga atgaacttcg taaagtagga
      901 cagaaaagtc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagttttc gagcaccaaa atacccattt
     1021 cttacggaag cgatgatgat tgatcgattg cttggctttt ctacgtccct gaaggaggcg
     1081 tatccctatt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacggct ttcgggaaaa gctccaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcgatgatct atccttacac caatggaaaa
     1261 atagaagcga agaataccca tataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat
//
LOCUS       DAZUKC010000209         1338 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000209 DAZUKC010000000
VERSION     DAZUKC010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.218"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            359..1270
                     /locus_tag="ACG80T_002860"
     CDS             359..1270
                     /locus_tag="ACG80T_002860"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4842435.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT          537 a          200 c          256 g          345 t
ORIGIN      
        1 aatatatcat aataaaaaca acaaaaaaat gtctggaaaa tcgaacataa aagtgtctga
       61 tttgaatatc ccagcagcgg aagaagaaaa acaaaatcga taacatttgt tctaagaatt
      121 agttacgtta aatacggaac gcaagataga cttgaaaaac ggtaacaaaa gaagtagtat
      181 attaaggtgt gatgtgctac ttgacattta ttctgctgag cgaactttct ggattgtgta
      241 gccttatatc tattcaggac gtatcgagca gattagtaaa ggtgttcttg atgattttca
      301 tcttgaacac ctttgttttt agcaattcgc aaaaaacgaa aagtaggaga attgataaat
      361 gaaaaaaaga tttttagcac ttgccatcgt actaggcact ggcttattgt caggctgtac
      421 gaatgccggt gaaaaaacag ccgttagtta taaaggcggt acaatcagcg aacaagaagt
      481 aatggatagc ttgaaaaaaa tgcaaggagc agattccgct gtgcaacagt tgatcgttta
      541 tcaagtattt gaagataaat atggagatga cgtatcaacc aaagaaatcg attctcaata
      601 tgatcaaaca aagaaacaat tgggtgattc atttgattca caattgaaat ctgcaggtta
      661 cacagaacaa acattcaaag acagtatcaa acaaagtcta gcctttcaag aaggtctaaa
      721 gaaacatatc aaattgaccg atgaagattt aaaaacggct tgggaatctt tccatccaga
      781 agtggaagct caaattatcc aagtagcaag tgaagatgat gcaaaagatg tgaagaaagc
      841 tgctgataag ggagacgatt tctctaaatt agccaaagac aagtcaactg atactacgac
      901 aaaagaagat ggcggaaaag tgaaatttga ttccactact acaacggttc cggcagaagt
      961 caaagaagca gcctttaagt tgaaagatgg tcaagtttct gatgtgatca cttctaccaa
     1021 tgcttcaact tatactacgg aatattatgt ggttaaaatg gtgaaaaacc aaaacaaagg
     1081 caacgacatg gataaataca aaaaagaatt gaaagaaatt gcaacagaca caaaattaag
     1141 tgacagtacc ttccagaata aagtaattgg cgaagtattg aaagacgcta atgtaaaaat
     1201 caaagacaaa gactttgaaa atgttttgag tacattcact tcagattcta gtacagcaag
     1261 tagtaaataa gcggtataaa taaaaaatat gaactattta aatcaataaa actgttcatc
     1321 taaatctttt tattagaa
//
LOCUS       DAZUKC010000210         1302 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000210 DAZUKC010000000
VERSION     DAZUKC010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.219"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            52..>1302
                     /locus_tag="ACG80T_002861"
     CDS             52..>1302
                     /locus_tag="ACG80T_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS4842436.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          403 a          260 c          252 g          387 t
ORIGIN      
        1 cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa gatgtctcat
       61 actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt cgaggaaaat
      121 tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg aactcttctg
      181 tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg
      241 gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac ttatctggag
      301 ctgaagaaac aacgatttaa atgtaagtct tgccaaagaa cattcgtagc ggatacctct
      361 gttgctgaaa aacactgttt cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta
      421 aaagagaata cttccatgac ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc
      481 tatcgtgtca tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac tttgccaaag
      541 gtcttatgct ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat gagttttatc
      601 atgatggacg gacaaagcca tgcattattg gatattgtcg aaaatcgacg cttgccttat
      661 ttagaacgct atttttctcg gttttcatta gcgactcgtg aagccgttca attgattgtt
      721 attgatatgt atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg
      781 atcatcgatc gtttccacat cgtccaacat attggcagaa ctttcttgaa tcatcgagtg
      841 aaagaaacca cggtacgact taaaggaaat tcccactctc aacgagggtt agggaaaaaa
      901 ttgaagcgct attggaaact cctacaaaaa gaagaagcaa agttaaacta tgaaaaacgg
      961 gtttggcgtg ccagttttaa agattatttg actgaaagtg agattgccga tcgactgtta
     1021 gctgcttgtc ccaacttacg ccaaggctat cagttatacc aagacattct ttatgcggta
     1081 aagaaaagag atcagtcact atttgaggat tgtttaacta gagacattag cggtctgcca
     1141 gaaacctata caaccacttt acgaacgttt aaaaagtttc tgccacagat ccaaaatgcg
     1201 cttcactaca gctattcaaa cggaccattg gaatgcttaa acaaccacat caaagtatta
     1261 aaacgcaatg cgtacggctt tcgcaacttt tacaatttca aa
//
LOCUS       DAZUKC010000211         1284 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000211 DAZUKC010000000
VERSION     DAZUKC010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.220"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..733)
                     /gene="wzy"
                     /locus_tag="ACG80T_002862"
     CDS             complement(<1..733)
                     /gene="wzy"
                     /locus_tag="ACG80T_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy"
                     /protein_id="HFS4842437.1"
                     /translation="MKEKINILMFSLTLVAFTIFCAFSLNQNYWLNWLGILIYLYAIC
                     TWHWRKGDSIFSLYTIFFTLFLLFSYGQCIMWAFGIGTDRGIGTTVVAYGTGIIPSES
                     DMIMVKWYSCISMFVFHIGALLFTRKTTLRKVSWYESGDADADRKFLFKIGCIISIIV
                     VPIVFVSKLLEVRIALVHGYNALYYGDYATQSGYLQIILYLFFPALICLLIGSNFSKV
                     ITRFVFIIFALYSLLGIMSGDRGSWL"
     gene            complement(752..>1284)
                     /locus_tag="ACG80T_002863"
     CDS             complement(752..>1284)
                     /locus_tag="ACG80T_002863"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4842438.1"
                     /translation="FITACRLDKQKNLPLMIDAIGKLIHEGYECELDIYGEGGLKTEL
                     ESYINHNGLTEFVHLKEFTNKIHEKMQNAAGFVISSDYEGISNSMLEALAIGVPVIAT
                     DCPVGGARMYLGDRNEIGHLVECNNLDKLTDAMRRVLLDRESVVRKAQKGIKLRKELT
                     SKNISNIWYEYLNQFF"
BASE COUNT          451 a          253 c          143 g          437 t
ORIGIN      
        1 acaaccaact tcctctgtct cctgacataa ttcctaataa agaatataaa gcaaaaataa
       61 taaaaacaaa tctcgttatt acttttgaaa agttgctacc aattaataaa catattaagg
      121 ctggaaaaaa aagatataga ataatttgta aatatccact ttgtgtagca taatcaccat
      181 aatataatgc attataacca tggactaatg ctatacgtac ctctagtaat ttagaaacaa
      241 aaactatcgg tactactatt atagaaatga tacaccctat tttaaataaa aactttctat
      301 cggcatcagc atctccagat tcataccaac taacttttct tagtgtggtt tttctcgtaa
      361 ataagagtgc tcctatgtga aatacaaaca tagaaataca agaataccat tttaccatta
      421 tcatatctga ttcactagga ataatccccg taccataagc tactactgtt gtaccgattc
      481 cacgatctgt tccaataccg aatgcccaca taatgcattg cccataacta aataataaaa
      541 aaagagtgaa aaaaatcgtg tataatgaaa atattgaatc tccttttctc caatgccatg
      601 tacagattgc atataagtaa atcaatattc caagccaatt tagccaatag ttctgattta
      661 atgaaaaagc gcaaaatatt gtaaacgcga ctaacgttaa cgaaaacatc aatatattta
      721 ttttttcttt cacacaatat cctccttttg gttaaaagaa ctggtttaaa tattcgtacc
      781 atatatttga aatattttta gatgtcagtt cttttcttag tttaattcct ttttgcgctt
      841 ttcttaccac actttctcta tccaaaagta ccctacgcat tgcatctgtt aacttatcta
      901 gattattaca ttcaacaaga tgacctattt catttctatc acctaaatac attcttgcgc
      961 cacctactgg acaatctgta gcgataactg gcactccaat agccagtgct tctaacatag
     1021 aattagatat accttcataa tcagaagaaa ttacaaatcc tgccgcattt tgcatctttt
     1081 catgtatttt atttgtaaat tcttttagat gaacaaattc tgttaatcca ttatggttga
     1141 tataactttc aagctcggtt tttaatcccc cttctccata aatatccagt tcacactcat
     1201 atccttcatg aataagtttt cctattgcat caatcattaa gggcaaattt ttttgtttat
     1261 ccaatctaca tgcagtaata aatt
//
LOCUS       DAZUKC010000212         1280 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000212 DAZUKC010000000
VERSION     DAZUKC010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.221"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            149..1021
                     /gene="scrK"
                     /locus_tag="ACG80T_002864"
     CDS             149..1021
                     /gene="scrK"
                     /locus_tag="ACG80T_002864"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFS4842439.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          392 a          229 c          290 g          369 t
ORIGIN      
        1 attattagaa ttttaaaaaa tgataaaaat tcaaaaagaa aaaatagtag ttatttactt
       61 gtcatgttac taaataaaaa aataatttaa atatgatata tagtcattta tttagtgatt
      121 tcattctaaa ataaagggag aaatgtctat gctatacggt gcaattgaag caggcggcac
      181 aaaatttgtg tgtgccattg gcgatgaaga aatgacgatc aaggaaagag tgagctttcc
      241 gacaacgaca cctgaggaaa caatgccttt agtcattgac ttcttcaaac aataccaagc
      301 agatttagca gggattggga ttggctcatt tgggcctatc gatatccatc gcgattccgc
      361 cacttatggg tacattacct cgaccccgaa attggcatgg caaaattttg atttcatcgg
      421 taccatgaaa aaagaatttc cgatcccgat ttcttggaca acagatgtca atgcggctgc
      481 ctatggtgag tatgtcttcg gcagtggcaa aggattgagc agtgtcgttt actatacgat
      541 cggtacgggt gttggcggcg gtgccttaca agaagggcgc tttatcgaag ggtttagtca
      601 tccagaaatg gggcatatgc tcgtcgtccc tcatccaaaa gatgactttg ctggcagctg
      661 tccatttcat ggcaactgtt tagaagggat ggccgctgga cccgccatcg aaaaccgctt
      721 agggaaaaaa ggacaagagg ttgcagaaga tgatccttat tgggaaatcg aagcctccta
      781 tattgctcaa tgtgcctaca atacaacctt gatgttttct ccagatgtga tcatttttgg
      841 cggtggtgtg atgaaacaac gccatatgct gcaaaacgtc cacgacgcat ttgcaaaatt
      901 ggtcaatggg tatgtcgaaa cacctgagtt aagcaaatat atcgtgaccc cagcattaga
      961 agacaacgct ggaacgctcg gttgtttggc attggctcga gaagcggtct tacattcata
     1021 aacgaaaaaa ggctcttcga catttagtgt tggtagagaa agatacaact gaaaatttag
     1081 tcataaccag ttaggcagag tgcttaactg gtttttgttt tacttggaag atcaagcctt
     1141 gaatgagata aattcttgct cgaaagttat ggaaatttcg atagccgtag gccacttgta
     1201 ttgtgcaata gttttttaca aaaaaaaggg ggtgcggata tttttttgga cctttttaac
     1261 aaatatatct ttgcttcttc
//
LOCUS       DAZUKC010000213         1274 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000213 DAZUKC010000000
VERSION     DAZUKC010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.222"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..416)
                     /locus_tag="ACG80T_002865"
     CDS             complement(<1..416)
                     /locus_tag="ACG80T_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842440.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYK"
     gene            complement(433..>1274)
                     /locus_tag="ACG80T_002866"
     CDS             complement(433..>1274)
                     /locus_tag="ACG80T_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFS4842441.1"
                     /translation="SLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKTIEQIKI
                     DNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDTIRSEKN
                     YSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEI
                     GKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNY
                     ISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFN
                     NIS"
BASE COUNT          437 a          219 c          140 g          478 t
ORIGIN      
        1 tacttataat ctattaactt gtagttgttt aacttttctg aaaaagttag acccacgtaa
       61 taatcttccc agctttctct ttcaattaac tgatccaatt tttgataatc cataaattca
      121 tcatgtatca aaaagaataa ataacttgat attttagcat ctagcttaat cagattaaat
      181 aatgtttgtt taaaattgct accattaata tgactgatag attctctagc ttcttcacat
      241 aaacttttta ctttattaat tactactttg tcatattcat ttaacacact attattacca
      301 ttacttagac gctcagattc tacgaaacaa ctcaaatcag aaatttccca aaaaaatttt
      361 ttgttaaaat ccaatttcct caactcctcc aaactacgta aatgatatag tctcataata
      421 aaataaaacc tctcagctaa tgttgttgaa attgtccact aaacctaatt gatattgctt
      481 aggagataca cctgcgtact ttttgaatac tctcgaaaag tgtgaagaat ctgaaaatcc
      541 taaacctaaa gaaacttcac tgatagtatg gtttgatttc aacattattt tagccgtaga
      601 aatttttcgc ttagagatat aattataaat cgtaatatgc atttcattct taaagctttc
      661 tcttaattta gtttcactta cagaaaaata attagctatt tcctttacag taattctttt
      721 atagatattt aagccgatga attgaacgac agaagaaatt aagggagaaa gatgctctat
      781 cttagttttt ccgatttcct tagtataata aacaatccca gaatccctca cttttaagac
      841 ctcaggtaac gatacagcta attcgttttc ttgtatcaaa gcatctctac tttgatacgc
      901 ttctattata tccattccta atgttataga cgtttgtgct aacttttcaa aaacaatgat
      961 agaataattt ttttcagacc gaatcgtatc tccactaaca cttggaatta ttccattact
     1021 cagattaaaa accatgtttt ctaaatttcg aatatctcca tttttaacat attccagtat
     1081 tttattttca taaagaaaca gatatatttc tggatcgtaa ttttgtgata acagattatc
     1141 tattttgatt tgctcgatag ttttacttag attttctgta tattcaatta acggttcatg
     1201 gaatagatct tctatttttc cagtaaaaaa ataatgaact aaaatcagaa tatctcgaat
     1261 gtctcctaaa gaat
//
LOCUS       DAZUKC010000214         1213 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000214 DAZUKC010000000
VERSION     DAZUKC010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.224"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..602
                     /locus_tag="ACG80T_002867"
     CDS             <1..602
                     /locus_tag="ACG80T_002867"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4842442.1"
                     /translation="NPLTLSGSSLISNLDKKIISFTARYDINIKGFDYLCEVAKNLPV
                     GWAIRIAGDGTEEERKELKRLIEKNGVLDKIICAGALFGDELINHYIESSIFISTSRS
                     EGLPLVLIEAMTYGLPVISFDHGGAKEILQDGNYGILIKNFDVQDMNNEIEKLSNNRL
                     LMEKYQKFSLERAKDFNEKKIIGEWQTLLNELGNEDVRN"
     gene            599..>1213
                     /locus_tag="ACG80T_002868"
     CDS             599..>1213
                     /locus_tag="ACG80T_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase domain-containing protein"
                     /protein_id="HFS4842443.1"
                     /translation="MRTSIKNFNLWKEWYLYIFFIGMIIRAINFYSLLPSTFDSIFFK
                     LIGISGVLLLGIDFIIKFNKRKVTYNILLIAYMVILVITSFVHKEYGLGENLKTIMWA
                     ILQYFLIYQFAFENSDNKRFFKRISWGFIYSWFVLVSMSMVLFFGKFGYEKYYNARHR
                     IRIGFLESRLFGTFSDINNAAIASLVVIVLILFYLYNKKVTGLNK"
BASE COUNT          438 a          106 c          225 g          444 t
ORIGIN      
        1 ataacccatt gacattaagc ggctctagtt taataagtaa ccttgataaa aaaattattt
       61 catttactgc cagatacgat ataaatatta aagggtttga ttatttatgt gaagtagcta
      121 aaaacttacc tgttggatgg gctataagga ttgctggaga tggtacagaa gaagaacgta
      181 aagagctgaa aagattaatt gagaaaaatg gtgtgttaga taaaattatt tgcgcaggtg
      241 cattatttgg agatgaatta atcaatcatt atatagaaag ttctattttc atatctactt
      301 ctagaagtga gggactacca ttagtgttga tagaagcaat gacttatgga ttacctgtaa
      361 taagttttga tcatggtggt gctaaggaaa tattacaaga tggaaattat ggaattttaa
      421 ttaagaattt tgacgtacaa gatatgaata atgagataga aaaattgtct aataatcgtt
      481 tacttatgga aaaatatcaa aaattctctt tagaaagagc taaggatttt aatgaaaaaa
      541 aaataattgg tgaatggcaa acattgttaa atgaattagg aaatgaggat gttagaaatt
      601 gaggacttcg attaaaaatt ttaatttatg gaaagaatgg tatctatata tattttttat
      661 agggatgatt attcgtgcaa taaattttta tagtttatta ccgtccactt ttgatagtat
      721 tttttttaaa cttataggaa tttcaggagt tttgctatta gggatagatt tcataattaa
      781 atttaataaa agaaaagtaa cttataacat tttattgata gcatatatgg tgattttagt
      841 aataacgagt tttgttcata aagaatatgg tctaggagaa aacttaaaga ctataatgtg
      901 ggcaatctta cagtattttt tgatttatca gtttgctttt gagaatagcg ataataaacg
      961 attttttaaa aggataagtt ggggatttat ttatagttgg tttgttttag tatcaatgtc
     1021 aatggttctt ttctttggta aattcggcta tgagaaatat tataatgcta gacataggat
     1081 cagaattggt tttttagaat caagactgtt tgggacgttt tctgatatta acaatgctgc
     1141 tatagcatct ttagttgtta ttgtcctaat cttattttat ctttataata aaaaagtgac
     1201 aggattaaat aaa
//
LOCUS       DAZUKC010000215         1147 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000215 DAZUKC010000000
VERSION     DAZUKC010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.225"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            34..>1147
                     /locus_tag="ACG80T_002869"
     CDS             34..>1147
                     /locus_tag="ACG80T_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase"
                     /protein_id="HFS4842444.1"
                     /translation="MERTARLKKNTIWALVFQITSIVCGMILLRLILTKYGSEVNGMI
                     SSINQFLQIISFLQLGVGAVMQSTLYKPLAEKDNLMISQILASGGKFYRQIAYILLVY
                     VGLLLAVYYVLVPNEFGRSYTMAMIATLSINSFAQYYFGIVNTLLLSADQKIYITYIA
                     QTIAFISNTVACALLISLNTEILMVKLITAIIYLACPIYYRWYVDRNYNIKRNVSYDK
                     EPIPQKWNGVAQHVASVVLDSTDVVVLSIFSNLIYVSIYNVYNLVIYGVKQLFMAMTN
                     GVQALLGELWARKEIEVLYKTFAWFEWIVHTLTVFIFGCTGILIVPFIQVYTRGVTDA
                     NYNQLSFAALLVTANALHCLRTPYHVMIKAAGKYKET"
BASE COUNT          378 a          171 c          205 g          393 t
ORIGIN      
        1 ataaaaagat aaaacaagtc aggagttgca aaaatggaaa gaactgctcg gctcaaaaaa
       61 aacactattt gggctctggt gtttcaaata acttctattg tctgtggtat gatcttgcta
      121 cgtttgatat taacaaagta cggatccgag gttaatggaa tgattagctc tattaaccaa
      181 tttctacaaa ttatttcttt tttacaatta ggagtaggcg ctgtaatgca atcaactcta
      241 tacaaaccac tagcagaaaa ggataattta atgataagtc aaatcttagc ttctggaggg
      301 aagttttatc ggcaaattgc atatattcta ctagtatacg tgggtttgtt acttgcagtc
      361 tattatgttc tcgttcctaa tgaatttggg agatcatata caatggcaat gattgcaaca
      421 ttaagtatta attcatttgc tcaatattat tttggtattg ttaatacttt gttattaagt
      481 gctgaccaga aaatttatat tacgtatatt gcacagacaa tagcatttat ttctaataca
      541 gttgcatgtg ctttattaat tagtctgaac acagagatcc ttatggtaaa acttattact
      601 gctataattt atcttgcttg tccaatatat tatagatggt atgtggatcg aaactataat
      661 ataaagagga atgtgtcata tgataaggaa ccgatccctc aaaaatggaa tggggttgca
      721 caacacgtag cttctgttgt actagatagt actgatgttg tagtgttatc tatattttct
      781 aacctgatat atgtatctat atataatgtg tacaatttag taatatacgg agtaaaacag
      841 ctattcatgg ctatgacaaa tggcgttcag gccttactag gggaactttg ggccagaaaa
      901 gaaatagaag ttctttacaa aacatttgca tggttcgaat ggattgtaca tactttgaca
      961 gtttttattt ttggctgtac cggtatacta atcgttccat ttattcaagt atatacaaga
     1021 ggagtaacag atgctaatta taatcaatta tcattcgcag ctttacttgt aactgctaac
     1081 gcacttcact gtctgcgtac accttatcat gtaatgataa aagcagctgg taaatataaa
     1141 gaaactc
//
LOCUS       DAZUKC010000216         1146 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000216 DAZUKC010000000
VERSION     DAZUKC010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.226"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..330
                     /locus_tag="ACG80T_002870"
     CDS             64..330
                     /locus_tag="ACG80T_002870"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFS4842445.1"
                     /translation="MTSTYASFDKDNLYYDFPSVSGFSSMNNKKYLLMLTRLGYSTSN
                     VRAKRNGGTVITDILLSNQYQVIPNQEIQHQATSGVKIIESEVS"
     gene            433..708
                     /locus_tag="ACG80T_002871"
     CDS             433..708
                     /locus_tag="ACG80T_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842446.1"
                     /translation="MTNLESRQFDPCWHQIDAQTKELLLGWEENQVVRDFSLNFIMID
                     YYNLATSMQRFPNYGMDIDSFKQKSFSGEIQNEESNNLVIPILYEPG"
     gene            718..924
                     /locus_tag="ACG80T_002872"
     CDS             718..924
                     /locus_tag="ACG80T_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842447.1"
                     /translation="MDGEEYPYKVAFDDLIQLSNLPKDSNMKIAGEFFAPGFLVGLVI
                     SSIATGCFLFLLFLDYIKKTEDIP"
BASE COUNT          394 a          184 c          204 g          364 t
ORIGIN      
        1 acaatcaacc aaactctaac agaatttgaa gtatatcaag aatattggga accactaaaa
       61 aaagtgactt caacttatgc atcctttgat aaagacaatc tctactatga tttcccctca
      121 gtatcaggat ttagttcaat gaacaataaa aaatatttac taatgttaac gagattaggg
      181 tactcaacaa gtaacgtccg tgctaaacgg aatggtggta cagttattac ggatattttg
      241 ctaagcaacc aataccaagt aattcctaat caagaaatcc agcatcaagc gactagcggt
      301 gtaaagatta tagaaagcga agtgagttaa acctcagata ttgcttcttt ttcatttgac
      361 gttctagaag taacaaaaga tgagttactt tatgtttatt ttcccgtcaa cttggccaat
      421 aatttgcact atgtgaccaa tttggaaagt cgacaatttg atccctgctg gcatcaaatt
      481 gatgctcaaa cgaaagaatt gttgctaggt tgggaagaaa atcaagtagt tcgtgatttc
      541 tcattaaatt ttattatgat tgattactat aatttggcga catcaatgca acggttcccg
      601 aattatggta tggacataga ttcattcaaa cagaaaagct tttctggaga aattcaaaat
      661 gaggaaagca ataatcttgt tatacctatt ctctatgaac caggttaggt ttttactgtg
      721 gacggggaag agtatcctta caaagtagct tttgacgatt tgattcaatt gtctaactta
      781 ccaaaagata gtaatatgaa aattgctgga gaatttttcg caccaggatt cttggtaggc
      841 ttggtaatct ctagtattgc aactgggtgc tttttatttt tactttttct ggattatatc
      901 aagaaaacag aggatattcc ttaggaaaat cacaatctaa atcttcccta gaaactatag
      961 aatgacaagg gggatataag caaattccta aatgattaaa tagggtgcgt tactagttag
     1021 ctccatagta agattttttc aaaattttct tgaggttgga tcaaaaaggg ctcttttcaa
     1081 ttagattaaa gacactcata caaaatagag aaacgaactt ataatcgcgt tcgtttccct
     1141 atttaa
//
LOCUS       DAZUKC010000217         1138 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000217 DAZUKC010000000
VERSION     DAZUKC010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.227"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..390
                     /locus_tag="ACG80T_002873"
     CDS             <1..390
                     /locus_tag="ACG80T_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4842448.1"
                     /translation="KIVVSNEEKYFYLQRNDSIMGKSFSMNNFDVIEAWQYNKEKILD
                     AFPDLHDSYYRRLCWAYFYVLDKMVLSSSYQQIPRKKTVIEFLKKNRNFILTYKGFTK
                     SRKIAIIALSFRVDWYRKLVEQKNKKN"
     gene            435..>1138
                     /locus_tag="ACG80T_002874"
     CDS             435..>1138
                     /locus_tag="ACG80T_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4842449.1"
                     /translation="MCRISIIVPVYNVEKYLKKCVDSILGQTFTDFELLLIDDGSTDN
                     SGSICDELAKTDNRIKVIHKENGGLSDARNIGIEVAKGDFIGFIDSDDYIDEDMYAFL
                     YNNILKYDAELSMCGIYDVHKNKEIKKLTPFSQLVTKSEAIELVLDGKLVVANAVSKL
                     YKKELFENVRYPKGKIAEDAAVILKIINQCNKIHVDTSQKYYYYHRGDSITSKKFTKK
                     DFDIIDVWNENEKWII"
BASE COUNT          456 a          107 c          206 g          369 t
ORIGIN      
        1 aaaattgttg tttccaatga agagaaatat ttctatcttc aaagaaatga tagtattatg
       61 ggaaaaagtt tttctatgaa taatttcgat gttatagaag catggcaata taacaaagag
      121 aagattttag atgcttttcc agatttgcat gattcctatt atcgccgact atgttgggca
      181 tatttttacg tgttggataa aatggtttta tcaagtagtt atcaacaaat tccacgtaaa
      241 aaaacagtaa ttgaattttt aaagaagaac agaaatttta tattaactta taagggattc
      301 actaaatcgc gcaaaatagc tattattgct ctgagcttta gagtagattg gtataggaaa
      361 ttggttgaac aaaaaaataa aaaaaattag aatgtttaaa ttagtaaata aaagaaaagg
      421 aattgaaaac taaaatgtgc agaataagta taatagttcc agtttataat gttgaaaaat
      481 atttaaaaaa gtgtgtcgat tcaattctag gccaaacatt taccgatttt gaattattat
      541 taattgatga tggttctaca gataattcag gaagtatatg cgatgaactt gcgaaaacag
      601 acaaccgaat aaaagtgata cataaggaaa atgggggtct aagtgatgcc agaaatattg
      661 gtattgaggt agcaaaggga gattttattg ggtttataga tagtgatgat tacattgatg
      721 aagatatgta tgcattttta tataacaata tattgaaata tgatgcagag ttatcaatgt
      781 gtggtattta tgacgtccat aagaataaag aaattaaaaa gttaactcct ttttctcaat
      841 tggttactaa atctgaagca attgaattag tattagatgg taagctagtt gttgctaatg
      901 cagtaagcaa gctatataaa aaagaattat ttgagaatgt gagatatcca aaaggaaaaa
      961 ttgctgaaga cgcagcagta atactgaaaa ttatcaatca gtgtaataaa atccatgttg
     1021 atacttctca aaaatattat tattatcatc gtggagatag tattactagt aaaaaattta
     1081 caaaaaaaga ttttgatata attgatgtat ggaatgagaa tgaaaaatgg attattaa
//
LOCUS       DAZUKC010000218         1135 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000218 DAZUKC010000000
VERSION     DAZUKC010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.228"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..350
                     /locus_tag="ACG80T_002875"
     CDS             <1..350
                     /locus_tag="ACG80T_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4842450.1"
                     /translation="EAVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFE
                     DAFQYTVQGNSHNRLKSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDE
                     WIYSSRKYINFDK"
     gene            407..799
                     /locus_tag="ACG80T_002876"
     CDS             407..799
                     /locus_tag="ACG80T_002876"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4842451.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            800..>1135
                     /locus_tag="ACG80T_002877"
     CDS             800..>1135
                     /locus_tag="ACG80T_002877"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4842452.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGI"
BASE COUNT          438 a          124 c          204 g          369 t
ORIGIN      
        1 gagaagctgt taaaggaatt tttaagttca cagatattaa cttagcgcgt gaggctaaaa
       61 atcgattgat tcatgattat atcgatcaac caaaatattc aaaagcttgc gcatcattgg
      121 atgatggatt cgaagacgcc tttcaatata ccgtacaagg aaattcccac aatcgactaa
      181 agagtaccaa tctaattgaa cgactgaatc aagaagtacg cagaagagaa aagattattc
      241 gcatcttccc caatcaaaca tcagccaatc gcttaattgg agccgttctt atggacctac
      301 atgatgaatg gatttattct tcaagaaaat acatcaattt tgataagtag aaatggtaaa
      361 aacattgtat agcattttac acaggagtct ggacttgact gagtttatgg aagaagtttt
      421 aattgatgat aatatggttt ttgatattga taatttaaaa ggatttctta atgataccag
      481 ttcatttggg tttatagcta aagaaaataa taaaattata ggatttgcat attgctatac
      541 acttttaaga cctgatggaa aaacaatgtt ttatttacac tcaataggaa tgttacctaa
      601 ctatcaagac aaaggttatg gttcaaaatt attatctttt attaaggaat attctaaaga
      661 gattggttgt tctgaaatgt ttttaataac tgataaaggt aatcctagag cttgccatgt
      721 atatgaaaaa ttaggtggta aaaatgatta taaagatgaa atagtatatg tatatgatta
      781 tgaaaaaggt gataaataaa tgaatatagt tgaaaatgaa atatgtataa gaactttaat
      841 agatgatgat tttcctttga tgttaaaatg gttaactgat gaaagagtat tagaatttta
      901 tggtggtaga gataaaaaat atacattaga atcattaaaa aaacattata cagagccttg
      961 ggaagatgaa gtttttagag taattattga atataacaat gttcctattg gatatggaca
     1021 aatatataaa atgtatgatg agttatatac tgattatcat tatccaaaaa ctgatgagat
     1081 agtctatggt atggatcaat ttataggaga gccaaattat tggagtaaag gaatt
//
LOCUS       DAZUKC010000219         1109 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000219 DAZUKC010000000
VERSION     DAZUKC010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.229"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(10..>1109)
                     /locus_tag="ACG80T_002878"
                     /note="aph(2'')-Ia"
     CDS             complement(10..>1109)
                     /locus_tag="ACG80T_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HFS4842453.1"
                     /translation="RYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIED
                     LPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVA
                     YLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGY
                     KEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEY
                     ILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTG
                     IIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYY
                     PIETIVYGIKNIKQEFIENGRKEIYKRAYKD"
BASE COUNT          368 a          185 c           83 g          473 t
ORIGIN      
        1 attatataat caatctttat aagccctttt ataaatttct tttctaccat tttcgataaa
       61 ttcctgttta atatttttaa ttccataaac aatagtttca ataggataat attcttcaac
      121 tatatcttga tattcttttg ctttctcaat atctatattt ccatacattc ttaatatatc
      181 ttctccaaaa tttgttccta tttcttcttc actatcttca agtaagtata taaaatcaca
      241 atattcatct ataattccag aatctccaaa atcaattatt ccagttaatc tattattgcc
      301 atctaacaat agatgattac aactaaaatc attatggcat aaacactttt taccctcaaa
      361 aactgttgtt gcatttagtc tttccataaa actttctata taatcttttt ctatatcagt
      421 taaatcatta taaatagttt cacgcaacaa tatatactct tctaatacat tttgtttatt
      481 atcaatagta cattcactaa tatctgtata atctaaaccg tgcatttgtc ttaaaaaact
      541 ggcaatatct cgttttaaca aattttgttc ttcttctgac atagtagaat aaatttctgg
      601 tgttaaaaaa gttcctttaa tttctttata acctagtata gataattcat cactaatata
      661 cgaatattca atattaggaa tttttacatt agtttctaaa tttgtattta aaaaattata
      721 tattgctttt tcttttgcat aacctttttt cttattagta ctaaattttg ttttaaaaat
      781 gtattcatta ttaactaaat atgccacact atcataacca ctaccgatta tttcaatact
      841 atctactttg aaattatcaa agtaatgctc aattaaatat ttcattgcct taacatttgt
      901 ggcattatca tcatatctat attccattaa ataacaatct tcttttttgc cctcgtgtaa
      961 ttcatgttct ggcaaatctt caataattct aaaaccagat ttttggtatg cccttattgc
     1021 tcttggatta tttttatgag ggtctaaaat aactgcatta gcatttcttt cttttttcaa
     1081 aaattcaaaa atcaatttaa tatatcttg
//
LOCUS       DAZUKC010000220         1104 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000220 DAZUKC010000000
VERSION     DAZUKC010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.230"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..483
                     /locus_tag="ACG80T_002879"
     CDS             <1..483
                     /locus_tag="ACG80T_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFS4842454.1"
                     /translation="MKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVYTSNE
                     LLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQYLEFL
                     LVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFLSILI
                     "
     gene            494..>1104
                     /locus_tag="ACG80T_002880"
     CDS             494..>1104
                     /locus_tag="ACG80T_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4842455.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYY"
BASE COUNT          377 a          150 c          183 g          394 t
ORIGIN      
        1 atgaaaatta ttgatgctat caagatagag ttttttacta tattttttgc gacatcctta
       61 tatattgtat ttcgaatgtt gccggtcgta atgatctcat ggttgttggt tagtgtctat
      121 actagcaatg aattattatc ctctttagaa aaaatacatt taccgaaaaa aataatgtta
      181 agtattacag tagtgatcag attctttccg acttatcgtt ctgaaataag aatgattaaa
      241 gaatctttaa aaatgagaaa cattcgcttg tctttttttc agcctctgca atatttggaa
      301 tttctgttag ttcccgttct tatacgtgca actatgattg ctgaagaaat gaccgctaat
      361 gcaataacaa aggggataga atctcctgtg aaaagaagtt cttactataa aacaaacatg
      421 agcttattag actatatcat cctgttttcc gttactattt ttttcttaag tattttgatt
      481 tgaggtgaaa gaattgtttg aatttaacaa tgtttctttt tcatataagg atgttaagga
      541 acaagcgctt gttgacctta atttttcaat aaaaaaaggc agtcttacat tgattactgg
      601 ttcttctggg agtgggaaat ccacactatt aaagttaatg aatggactta ttccaagact
      661 ttatgacgga aaattggttg gggaaatttt atttgaaaat aactcgctac aatccttaac
      721 acaagaaagt atatcaaaaa atattggcta cgtaagtcaa gatcctagag gacaattctt
      781 tacaacaaat acaacgagtg agttagtatt ttcgatggaa aactttggat ttacaaaaga
      841 gcatatggat aaaagaatca atgaattagc tgaattatta gatctttcaa atatattaga
      901 taaaaacatt ttttcgctat caagtggtga acggcagaaa atttctattg gatgtagttt
      961 atcgctaaac tctaaattaa ttatattgga tgaaccgtct tcaaatttgg acttcaaagc
     1021 gactaagaaa cttgctgact tactttgtga tttaaaaaat aaaggattta ctattgttat
     1081 agctgaacat cgtttttact attt
//
LOCUS       DAZUKC010000221         1097 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000221 DAZUKC010000000
VERSION     DAZUKC010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.231"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1032
                     /locus_tag="ACG80T_002881"
     CDS             <1..1032
                     /locus_tag="ACG80T_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HFS4842456.1"
                     /translation="TRFLCHDCGRTFSAKTDLVDEHHQLTKELKQAILMELYENQSRK
                     LIAKKYFVSDGTVTRILREATKHYQSRMNFLPTVLCMDEFKSMKSVSGSMSFICVDGT
                     THQLFTILEDRRLYKLTQYFLHFPRKARLKVKYLVMDMNASYCQLLKTVFPNAEIVTD
                     RFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYRRLKRYWKLLLKDSTTWEPLKRHY
                     HRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLRYYFVKRGTEGFQETLKTISSTLP
                     QSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNNKIKLIKRIAFGYRNFYNFRARIY
                     LQQGLIFEN"
BASE COUNT          337 a          240 c          211 g          309 t
ORIGIN      
        1 acccgttttc tctgccatga ttgtggaagg accttttctg cgaaaacgga cttagttgat
       61 gaacatcatc aactaacaaa ggaactgaag caggcaattc tcatggaact gtacgaaaat
      121 caatcacgta aactaatcgc taagaagtac ttcgtctcgg atgggacggt gacacggatc
      181 ttgcgtgaag caacgaagca ttaccagtca cgtatgaact tccttccgac ggtgttgtgt
      241 atggatgagt tcaaatcaat gaagtctgtt tctggttcca tgagttttat ttgtgttgat
      301 gggacaaccc atcagctatt tacgatcctt gaagatcgca gactttacaa actaactcaa
      361 tacttcctgc actttcctag aaaagcccgt ttaaaggtca agtacctagt gatggacatg
      421 aatgccagct attgtcagtt gcttaaaaca gtctttccaa atgcggagat cgtaactgac
      481 cgctttcata ttgtccaaca catcaatcgc agcttcaatc aattacgtgt ccagatcatg
      541 aatcgttttc gaacctcaca ctctgaaaat cagaagaagt accgtcggct caagcgatat
      601 tggaaactct tactcaaaga ttccacgaca tgggagccac tcaaacggca ttatcatcga
      661 ctgtttaaac gaccaattag tcaaacagaa atcgttgatg agctgttaag ctacaacgaa
      721 gaattgcgaa ctgcgtatca tttttgtcaa ttattacgct attatttcgt taaacgaggt
      781 acggaaggct ttcaagaaac ccttaaaaca atttcatcta cgttacctca gtcgtttaaa
      841 aagaaattca cgatcttcca ttgctaccaa tcgggtattt caaatgcctt taaggtgtcc
      901 cactcaaacg gcgtcactga ggggctgaat aacaagatca agctgattaa acgaattgcg
      961 tttggttatc gcaactttta taactttcgg gcacggatct atttacaaca aggtctgatc
     1021 tttgaaaact aaaaaaatat gcggcgcaca gaacgcgccg catatcggac taagttttat
     1081 tcaaatttgt cttcacc
//
LOCUS       DAZUKC010000222         1073 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000222 DAZUKC010000000
VERSION     DAZUKC010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.233"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1073
                     /locus_tag="ACG80T_002882"
     rRNA            <1..>1073
                     /locus_tag="ACG80T_002882"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          329 a          214 c          308 g          222 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
     1021 ccggggctaa acatattacc gaagctgtgg agtacacctt taggtgtatt ggt
//
LOCUS       DAZUKC010000223         1071 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000223 DAZUKC010000000
VERSION     DAZUKC010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.234"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>1071)
                     /locus_tag="ACG80T_002883"
     CDS             complement(<1..>1071)
                     /locus_tag="ACG80T_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HFS4842457.1"
                     /translation="FPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTDLEGT
                     SAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKTKGNV
                     LWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWKQKGL
                     VFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVLFEYS
                     RSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDALASAT
                     LSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEMAAGA
                     AVNSSIIESLKGIETIKAYSGE"
BASE COUNT          383 a          204 c          150 g          334 t
ORIGIN      
        1 cttcaccgct atatgcttta attgtttcta ttcctttaag actttctatg atactagaat
       61 ttacagctgc accggctgcc atttcttccc tgttcgattt ttcatagctt ttcacgaaag
      121 cgaatattgc aactacataa aaaggcaaag agcatagagt gattaaaaac aacgtactat
      181 tctgaatagc taacgtaacc ccaacaagca acaccatacc aatatctaaa aatattgata
      241 acgtagcact tgctaaagca tcaataatct tactggcatc aagaaaacga gaaataattt
      301 cccctgattt tctcgtagca aaaaaattca ttggtaaagt taacacatgt ttgaaatact
      361 gaagcataat tgccatactc attcgctgtc ctaaaataat aagaagataa ctacgactat
      421 attcaaacaa tacccgaaat aaatagacag cgatcaagcc aatggaaatt atatttaaag
      481 ttgaacgtgc ttgattaggg atgaaaaaat caagaatacc ttgaaaataa tatgagcttc
      541 ctatgccaaa aaacgtaatg aaaatcgatg ctaaaacgat gtggaatact aatccttttt
      601 gtttccaaat tataggcaaa aaagaactta atccagctac tttttcttta ataggaatat
      661 aagtttctct aggcataggt aataaaagaa tacctgtcca ttctttttga aacgcttcaa
      721 tcgtttttct attttttcca ttagcgggat ctgcaatcca tagtacgttt cccttcgttt
      781 tataaattac aacgtagtgc atatagctgt tatcaaccaa tatatgtgca attaatggta
      841 agataagatc tctttctttc cagatctcac tatctgcttg aatagccata caatcaaaag
      901 acaatttttc aaatgtcttt ttcaatccaa atgctgaagt accctctaaa tcagtcccag
      961 ataggtcccg taatttatga atcgggatat cagttttaaa atattttaaa atcatcgaaa
     1021 ggcaagcaac accacaatct ttttcatctt gttgctgcac aaaaggaaat g
//
LOCUS       DAZUKC010000224          978 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000224 DAZUKC010000000
VERSION     DAZUKC010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.235"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(301..387)
                     /locus_tag="ACG80T_002884"
                     /pseudo
     CDS             complement(301..387)
                     /locus_tag="ACG80T_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          345 a          151 c          127 g          355 t
ORIGIN      
        1 tgtctgctac cccggcctaa tctggcaagg ggaggaggag atataatgaa ataccttttt
       61 gattttgttt ttgcaatcaa gccacttgca gatgtagtgt gcagttacct ctgcaaatgg
      121 cttgattgca aaattgccaa aaaagacagt aagcctaccc gttaattcta taatatagaa
      181 acgtaaaaaa aaccccatca gcctgcacgc tgatggggtt tggtgatata atgaaatacc
      241 tctttgttac tcataattat accataatta gtcacgaatg caaccaatag aaaaaactac
      301 ttagaacggt agatcatcgc taataacaat gcattgtcca ccaaatggat tttgtgaagc
      361 attaaataca ttgttattaa ttgtcgctgc ttttaactag gcctctcccc ttctattata
      421 aaagaagata tatatcattt cttaattacc tactacctta aaccaaaatt taagtactac
      481 ctaatttaag tagtacttaa acaatctaaa aaatagttat cccaattgtt atgcttttat
      541 aatacgatta tttgtaacaa aatgaatatt atataattaa acactaccat atttaggtag
      601 tgtttaatta ttagcaaaac aaaaaatatt tattctattc tcttaacttc aactaagtta
      661 tgctgcacac ctttatataa atcatatgca actcattagc tagacaaact acaagcatgt
      721 agttttatct gttaattatg tactacctaa ataaagtagt ttataattag caagtatata
      781 tatttaaaaa tttaagatta atttttttat gacttgtaca aatttatctg atattctctt
      841 attaagaact acctaatttg ggtagttctt attttttaca ttttaagtag tatttaattg
      901 caatttggta gtattttttt attatcttat tcttagaacg gtagtactta atataaatat
      961 aggtagtatc taatttaa
//
LOCUS       DAZUKC010000225          924 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000225 DAZUKC010000000
VERSION     DAZUKC010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.236"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            105..>924
                     /locus_tag="ACG80T_002885"
     CDS             105..>924
                     /locus_tag="ACG80T_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS4842458.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDP"
BASE COUNT          301 a          183 c          174 g          266 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaa
//
LOCUS       DAZUKC010000226          918 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000226 DAZUKC010000000
VERSION     DAZUKC010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.237"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(128..676)
                     /gene="aac(6')"
                     /locus_tag="ACG80T_002886"
     CDS             complement(128..676)
                     /gene="aac(6')"
                     /locus_tag="ACG80T_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFS4842459.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          266 a          190 c          139 g          323 t
ORIGIN      
        1 ggtacacggt gttccaaaag tagcttcttc ggaaataagt tggaatttca caaaaattca
       61 agaagtaatt ttcgtaaatt ccttcttatt tcttgaagct gattacttct gtcacaacct
      121 ctttttatta ttgagaattt ggtcgaggaa taatcgtttt tgccatccaa atatccggtt
      181 tgtcccagcc atttgcattt ggtaagacac ctacgatttt ataacctaat ttttcataga
      241 attcatacgg atgttcacga aggttctgga tggaagccac tttatcaaat gtatgctcat
      301 acaggtccgt ttgacttaac gttgttccat ggtctaaatc atccgtacct aaataaatcg
      361 tgattcctcc tctggaagct acttcttttt ctaagtaatt gactaatcga gtacctattt
      421 ggttctttcg tcgggagctt tctacaacta atggatgcaa ttcccaacct gtgataccgt
      481 attgagggat tgcaccaata aatcctacta actcatcttg gtctaccgct gctaccgcga
      541 ttcgttctgg attcatcatt tcttctactt cttctgccga gctgtctcca tattcttccg
      601 gccaagtcag tctcagtaaa tcagaaagct gatctttcaa tactggatta ttacggtcaa
      661 attcactgat tatcatacgt cttccccctt gttcttgctt attctaacag tccttttgaa
      721 aaaacgaaat gttctcgctc aaaaatcttt agctaccaat cagtatctct attctttcat
      781 tatttatctt acttataaaa taaaaaaatt atacagaaac actttattca aaataggaaa
      841 gatcaggaaa gccatcaatt ggaggaaaaa actctgtttg ttcatcatga tatagattat
      901 cttttgcttt aaaaaaaa
//
LOCUS       DAZUKC010000227          905 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000227 DAZUKC010000000
VERSION     DAZUKC010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.238"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..286
                     /locus_tag="ACG80T_002887"
     CDS             <1..286
                     /locus_tag="ACG80T_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HFS4842460.1"
                     /translation="LFLFNKFYEGQEVRHIGVTYSRLVYTDSLQLDLFSDPRKKIAEE
                     NLDRIIDKIRQKYGFTAIIHASSMLEGARSITRSTLIGGHAGGLGGLHND"
     gene            279..626
                     /locus_tag="ACG80T_002888"
     CDS             279..626
                     /locus_tag="ACG80T_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842461.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFAELLKWSQI"
     gene            623..805
                     /locus_tag="ACG80T_002889"
     CDS             623..805
                     /locus_tag="ACG80T_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842462.1"
                     /translation="MNIIKKYEFGYITYEQLIEELWGYGQQLINEVGIDCFCFYIESG
                     AGYHKNRYYIFPYPPK"
BASE COUNT          342 a          106 c          176 g          281 t
ORIGIN      
        1 tcttttttta ttcaataagt tttatgaggg gcaggaagta cgacatatag gagtaactta
       61 tagcagatta gtatacacag attccctaca attagattta tttagtgatc caagaaaaaa
      121 gattgctgaa gaaaatttgg ataggattat tgataaaatt agacaaaaat atggatttac
      181 agctattata catgcttcat ctatgttaga aggtgcaaga agtattacga gaagcacttt
      241 aataggagga cacgcaggag gattaggagg tttgcataat gattgaatat aacgatagag
      301 gacgtttaaa atggtcggga ttttatttgt ctgatcatac tgaaagaatc aatgcagaca
      361 atgtacaacg tgctaatcat aatttggcaa aagaacaaat gacgactgaa gagattgcga
      421 aaatactgag aactgctgtc ataaaaaata gacccgtctg cattcaaaaa gaggaacgaa
      481 acgctgaagg ttactatcca gatgatgtag taggaaaaat tattggatat gacgagcttg
      541 gtgtatttgt ggaagaagaa aaagttcact atgatgaaat taggaatgtt tctttcgcgg
      601 aattattaaa atggagtcag atatgaacat tattaaaaaa tacgaattcg gttatattac
      661 gtatgaacag ctaatagagg aattatgggg atatggtcaa caattaatta atgaagtggg
      721 gattgattgc ttttgtttct atattgaatc cggagcagga taccataaga acagatacta
      781 tatatttcca tatccaccaa aatagtctct tagagatttt taaattttct ccaaatactt
      841 tcaattattg tgaaatattt ccttttaatt aatcaaattt tttataaaag taatatataa
      901 aataa
//
LOCUS       DAZUKC010000228          882 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000228 DAZUKC010000000
VERSION     DAZUKC010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.240"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            117..416
                     /locus_tag="ACG80T_002890"
     CDS             117..416
                     /locus_tag="ACG80T_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4842463.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGENIGR"
     gene            681..>882
                     /locus_tag="ACG80T_002891"
     CDS             681..>882
                     /locus_tag="ACG80T_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842464.1"
                     /translation="MNAIVILAEITYWYRPKKIFNDDGQLLGYQKKFLEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKG"
BASE COUNT          369 a           87 c          176 g          250 t
ORIGIN      
        1 atgatacttt attcttataa atacaaataa atacattttt ttacgcaatt tgtatttatt
       61 tgtacttata aaggtagagg agttatagaa taattaatta aaaaggagag gtttttgtgg
      121 aagcaataaa attaatgatt tcagaagaac aggaggaaat gtttctaaag cagttttatc
      181 aaattgctac aaaagctatt gaaaaggctc aagaaaatgc ggggatgaca agggaatatc
      241 tcaatcaaaa agaaatggcg gaatacttaa atgtgagtga aaacttttta aaaaatgctg
      301 tgagaaatga gggattgccc gtagttgaat taggggcaag aaaattttat tcgaaaaagg
      361 cagttaatga gtttatgttt gctcgaatga aaagagggga aaatattgga agatgatgaa
      421 gtagaaatta taaaaaaatg ctttaaaaaa agaaaaaaag tgttcccaaa agtttgcaga
      481 ctgggggaac actcaataat aaagtgctga gaaaacttta tttatagcta ttatatcaaa
      541 aaagaaggtg aaaaggtgtt ttcttctgga aatgttgtag ttgacaaact tagagaaata
      601 aattttgacg gaaatgtaat ccacacacat ggtacgatca attaagaaaa aaaacgaaaa
      661 aaggtgttga gaagccctat ttgaatgcaa tagttatctt agcagaaatt acctattggt
      721 atagacctaa aaaaattttt aacgatgatg gtcagctact tggttatcaa aaaaagtttc
      781 ttgaagatat tttgcaaaaa tcatataagc aattaagcaa aaaaacagga ttatcagaac
      841 gagctataaa agatgcaatt gtgtacttag agaaaaaggg ga
//
LOCUS       DAZUKC010000229          871 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000229 DAZUKC010000000
VERSION     DAZUKC010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.241"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            182..418
                     /locus_tag="ACG80T_002892"
     CDS             182..418
                     /locus_tag="ACG80T_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFS4842465.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            437..652
                     /locus_tag="ACG80T_002893"
     CDS             437..652
                     /locus_tag="ACG80T_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HFS4842466.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
BASE COUNT          284 a          116 c          198 g          273 t
ORIGIN      
        1 ctcataattc cacaaaaact ttgaaaacag ttcttgaaca ctaacttttt cattcctgaa
       61 tcaaatttat tttggaaatt ttactattca agaataaact tgataagttt caggaagatt
      121 tttactgtta atcaaatacg tatgttacag tagcaccgta aaaaatatta tttaaagaag
      181 cgtgtttaaa catgcaaaat gtaaaggaac taagtattgt ggaaatgcac aaactgttgg
      241 tggaggcaat aatgcttggg gaaaattggg gcaagttgta ggtggactta ccacaggggc
      301 agtaggtggt gcaggtttag gaacagcaat ttgcggacca gcttgcggtg ttgttggagg
      361 gttatatgga gctgtcgctg gaggtgctgc agctggctgg gatgctcgaa aaaaataaaa
      421 aaggagaata agtagtatgc atattaaaaa cacaaaaaca acatttatat tatcttcaga
      481 agagttgaaa aatattcaag gtgggagtgc cgtgggagtt ttaggcacta ctttttctgg
      541 ggcaacagca ggagtaaaac tttgttcagc cggaggacct tatgcaatag ctgcttgtgg
      601 tgtaggtgga gctttattag gtgctggatt cagcatgtgg accggtgcgt aaacaattaa
      661 aaaatatcaa acctcaagat tggtttgtgt cgattacgaa cataggtatg ttctttatct
      721 tttcaaaagt ttggaatgga cagaactttt tactaaagct tcttgtgcta gtttttataa
      781 ttatattaaa tttttatctt ttattaaagt cagataaaaa ttctttggaa atgtaatggt
      841 agagatattt ttacaaaagg acttgcagcg c
//
LOCUS       DAZUKC010000230          867 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000230 DAZUKC010000000
VERSION     DAZUKC010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.243"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            81..>867
                     /locus_tag="ACG80T_002894"
     CDS             81..>867
                     /locus_tag="ACG80T_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HFS4842467.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNI"
BASE COUNT          321 a          160 c          161 g          225 t
ORIGIN      
        1 taaaagtaaa aaggccatat aacagtcctt ttacggtaca atgtttttaa cgacaaaaac
       61 atacccagga ggacttttac atgacccaag tacattttac actgaaaagc gaagagattc
      121 aaagcattat tgaatattct gtaaaggatg acgtttctaa aaatatttta acaacggtat
      181 ttaatcaact aatggaaaat caacgaacag aatatattca agcaaaagaa tatgaacgaa
      241 cagaaaaccg acaaagtcaa cgaaatggct attatgagcg cagctttacg acacgtgtag
      301 gcacgctaga attaaaagta cccagaacac gtgatggcca tttttcaccc acagtgtttg
      361 aacgttatca acgaaacgaa aaagccctca tggcttcaat gttggaaatg tatgtatcag
      421 gcgtttcaac tcgtaaagta tcaaaaattg tggaagaact ttgtggtaaa tccgtctcta
      481 agtccttcgt ttctagctta acagaacagc tagaacctat ggttaacgag tggcagaatc
      541 gtttattatc agaaaaaaat tatccttact taatgaccga tgtactctat ataaaagtac
      601 gagaagaaaa tcgagtactc tcaaaaagct gtcatatagc gattggaata accaaagatg
      661 gcgaccgtga aattatcggc ttcatgattc aaagtggcga aagcgaagag acctggacaa
      721 cattttttga atacctaaaa gaacgcggtt tacaaggtac ggaactcgtt atttctgatg
      781 cgcacaaagg attagtctct gccattagaa aatccttcac caacgtaagt tggcaaagat
      841 gccaagttca cttcctaaga aatatct
//
LOCUS       DAZUKC010000231          855 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000231 DAZUKC010000000
VERSION     DAZUKC010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.246"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..491
                     /locus_tag="ACG80T_002895"
     CDS             <1..491
                     /locus_tag="ACG80T_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4842468.1"
                     /translation="KIIFAEDNWFQLQVFMTNTRICFVDKLCYYYRQQNNSIMHKPKS
                     NLVFRHGNMIADYEKLIRQNGNNIIDKKACMLVAFDVLIMEIFNQKNVGGTCEDYKNA
                     LVSLKHHKILRENISNIVRMKAYELEPNMRKKLALVTISIMFNFKMYKTAYNMLWRIY
                     NR"
     gene            505..>855
                     /locus_tag="ACG80T_002896"
     CDS             505..>855
                     /locus_tag="ACG80T_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4842469.1"
                     /translation="MRKLTFVIGIMGKGGAERVIANLANNFIQDNWEIDIITIYGNRQ
                     DYELNRKIQVHPIICKSSFRAFRPLERFKELRKKIREIQPDLVISFLADVNIHVIISL
                     LGVKQKLIVSERNDP"
BASE COUNT          338 a           88 c          160 g          269 t
ORIGIN      
        1 acaaaataat ttttgcagaa gataattggt ttcaattaca agtatttatg acaaatacaa
       61 gaatctgttt cgtagataaa ttgtgttatt attatcgcca acaaaataat tctataatgc
      121 ataagccaaa aagtaatttg gtttttagac atggaaatat gattgctgat tatgaaaagt
      181 tgataaggca aaatggaaac aacataatag ataaaaaagc ttgtatgttg gttgcatttg
      241 atgtactaat tatggagatt tttaatcaaa aaaacgttgg tggaacatgt gaagattata
      301 agaatgcatt agtttctttg aaacatcata aaatattgag agaaaatatt tcaaatatag
      361 ttagaatgaa agcatatgaa cttgaaccta atatgagaaa gaagttagcc cttgtgacca
      421 ttagtataat gtttaatttt aaaatgtata aaacagcgta taatatgctc tggagaatat
      481 ataatcgata aagaggtagg aaatatgagg aaattgacct ttgtgatagg aattatggga
      541 aaaggagggg cagaacgagt aattgcgaat ttggcaaata attttattca agataactgg
      601 gaaattgata ttataactat atatgggaat cggcaagact atgaactgaa tagaaaaata
      661 caggtacatc caataatatg taaaagttcg tttcgtgcat ttagaccttt ggaacgcttt
      721 aaggaactta ggaaaaaaat aagggaaata cagcctgatc tggttatttc gttccttgct
      781 gatgtaaata tccatgtcat tatttcattg ttaggagtaa aacaaaaatt aattgtttct
      841 gaacgaaatg atcca
//
LOCUS       DAZUKC010000232          802 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000232 DAZUKC010000000
VERSION     DAZUKC010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.247"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>802
                     /locus_tag="ACG80T_002897"
     CDS             <1..>802
                     /locus_tag="ACG80T_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFS4842470.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSW"
BASE COUNT          272 a          152 c          150 g          228 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aa
//
LOCUS       DAZUKC010000233          776 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000233 DAZUKC010000000
VERSION     DAZUKC010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.248"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            42..743
                     /locus_tag="ACG80T_002898"
     CDS             42..743
                     /locus_tag="ACG80T_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS4842471.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT          274 a          144 c          137 g          221 t
ORIGIN      
        1 gtgacaataa atatagtgaa caataaggag aggacttttt aatgcgtgtt aataaaatcg
       61 attttcactt ttcaaaactc agatatcaac ctgattttga acatttctcg gatgaagagt
      121 tccaacagat caaggaacat actgttcttc gttcttataa aaaaggacag atccttttcg
      181 acgatggtga tcccagacaa cggttgtata tcttgaccaa aggactcgtt cgtttagaac
      241 gttatgatga aagtgcaact tattactact atgactatgt gaatacaaat aacctttttc
      301 ctatgtcagg aatgtttgga gaagagacct acacttatac agctcaagcg atgactgata
      361 ttgaaacatt ctaccttcct gtttcctact atgaaaaatt agcacgaaaa aatcccgaac
      421 agttgatcta tatgatacaa aaaatttcta aagtagttga aatacatgaa ttgcgtatcc
      481 aaatcggatt gacctctagc gcctttgacc gggtaaaaca aaatttattc attttgaaag
      541 aagaattggg cgtaccaaca aaagatggtc atatcaccat tccttatccg atcaccttaa
      601 aagaattagc agtgaatagc ggcacgacac gtgaaactgc tggacaagta atcaaacaac
      661 tacgtgattc cggactgtta gattatgaca aaaagcgttt tactttctgt aagacagcta
      721 ctactccagc tatgagagaa taacgcgaac aaaaataagc agtgaccctg atattt
//
LOCUS       DAZUKC010000234          749 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000234 DAZUKC010000000
VERSION     DAZUKC010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.250"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..231
                     /locus_tag="ACG80T_002899"
     CDS             <1..231
                     /locus_tag="ACG80T_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842472.1"
                     /translation="IGFRTKRSLSSKKNWVYSQTIFYGGIVLISLLSSTLYSLNIIDV
                     STSNSISIIGIIIAAIITQLFLVFGEKKRSKK"
     gene            complement(322..687)
                     /locus_tag="ACG80T_002900"
     CDS             complement(322..687)
                     /locus_tag="ACG80T_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842473.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
BASE COUNT          281 a          101 c          132 g          235 t
ORIGIN      
        1 ataggtttta gaactaaacg aagtctgtct tctaaaaaaa attgggtata ctcacaaact
       61 attttttatg gaggaattgt tttaattagt ttactttctt caacacttta ttctttgaat
      121 attatagatg tttccacctc aaattcaata tctattattg gaatcattat tgctgcaatt
      181 attacacaac tgtttttagt gtttggagag aagaaaagaa gtaaaaaata gaaaaactag
      241 tgatagtttt ggtgatataa gaaaaacata tatgtataaa aagaagaaag tagcttctct
      301 ttgtacatat atggtttgtt tttatggatg ccgagctttt ttataagata aggctaccgc
      361 ccataacgac ccaaggattt gtggaactaa gtaaatacct gcaagaagca agatcagctg
      421 ggtcggtttt aaaggatgat agaagtaacg ccattcagta aaattggcgc aaaataaata
      481 ggaagtgaca gtagatataa tattggcaat gaataaagat ataatacgcc tgttcctttg
      541 aaaataatat ccagcaaaaa gagataaaaa tatcaatgtg atgaacccgc ttacggaatg
      601 ataacgaaaa tcaagcagta aggcgaaatg cacataagga acaaaagata aaagcaacgg
      661 aaggtttttc cagcaagttt ttatcatctt tcatcaccac ctaacaagtg ttattatttt
      721 gttagtttta ttgtaaattg aatcctgca
//
LOCUS       DAZUKC010000235          741 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000235 DAZUKC010000000
VERSION     DAZUKC010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.251"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>741)
                     /locus_tag="ACG80T_002901"
     CDS             complement(<1..>741)
                     /locus_tag="ACG80T_002901"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFS4842474.1"
                     /translation="FPNAKEIFKALDDSTIKKVGIIGAGYIGVEIAEAIRKRGKEVYL
                     FDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYLGRKKIEKIVTTSGTQYS
                     MDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKDIYAIGDCATTFFTSINR
                     SSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFNYNFYAAGLNLKTARLLS
                     ESIDYIEIEDFIRPAFLRKNSRVKLRIS"
BASE COUNT          241 a          131 c           93 g          276 t
ORIGIN      
        1 aagctaattc ttaattttac acgactattt tttctcaaaa aagcaggtct aatgaaatct
       61 tctatttcta tataatctat agactcactt aataatcgtg ctgtttttaa attcaaacct
      121 gctgcgtaaa aattgtaatt aaatatagaa aagccatttg taaattggga accagaaggt
      181 gtaaaatctt gattgttaat atgttgagtt gcaatataag ctgttcttag agcattagaa
      241 atagaaaaat caacactact tctattaatg gacgtaaaaa aagttgtagc acaatcccca
      301 atagcatata tatcttttat atttgtttgc tggtattcat tagttagata tgctccacta
      361 acatctctca agagttcctt ttccccaatt ggactattag atagaaatcc tatagcttgt
      421 acgaccatat ccatagagta ttgggtacca cttgtagtga ctattttttc aatctttttt
      481 cttcctaaat attttttagg actttctgat agacaaagat gtattccatt ttttgatagt
      541 atttcttcaa ctttatctga aaaactacgg tcgtaataag tcgacaacac tctatctgca
      601 acatcgaaaa gatatacttc tttccctctt tttcttatgg cttcagcaat ttcaactcca
      661 atatatcctg ctccaataat tcctactttt ttaatagtac tatcatccaa agctttaaaa
      721 atctcttttg catttggaaa a
//
LOCUS       DAZUKC010000236          717 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000236 DAZUKC010000000
VERSION     DAZUKC010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.253"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            26..706
                     /locus_tag="ACG80T_002902"
     CDS             26..706
                     /locus_tag="ACG80T_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFS4842475.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEI
                     KVLLGIPA"
BASE COUNT          253 a          123 c          145 g          196 t
ORIGIN      
        1 gaaaaagata gcaggaggaa tgacgatgaa tcattttaaa ggaaagcaat ttcagcagga
       61 tgtgattatt gtagccgtgg gctactatct tcgttataac cttagctatc gtgaagttca
      121 agaaatctta tatgatcgtg gcattaacgt ttctcatacg acgatttatc gttgggtgca
      181 agaatatggc aaactactct atcaaatttg gaaaaagaaa aataaaaaat ccttttattc
      241 atggaaaatg gatgaaacgt acatcaaaat taaaggaaaa tggcattatt tgtatcgagc
      301 catcgatgca gatggtttaa ccttggatat ttggttacgt aaaaaacggg acacacaagc
      361 agcctatgct tttcttaagc ggttagtgaa gcagtttgat gaaccgaagg ttgtagtcac
      421 agataaagcc ccctctatta caagtgcctt taagaaacta aaagaatacg gcttttatca
      481 agggacagaa catcgtacca ttaaatacct gaataatttg attgaacaag accatcgtcc
      541 agtaaagaga cgcaataaat tctatcgaag tttacgcact gcctctacca cgattaaagg
      601 catggaagcc attcgaggat tatataagaa aacccgaaaa gaaggcactc tcttcgggtt
      661 ttcggtctgt actgaaatca aggtattatt gggaatccca gcttaaatca tagatac
//
LOCUS       DAZUKC010000237          707 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000237 DAZUKC010000000
VERSION     DAZUKC010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.254"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(64..639)
                     /locus_tag="ACG80T_002903"
     CDS             complement(64..639)
                     /locus_tag="ACG80T_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFS4842476.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          237 a          152 c          125 g          193 t
ORIGIN      
        1 caaataataa aatgaaaaaa cagagccaga ttagcattat tttgctaatc cagctctgtt
       61 tattcagcct atttttcttt ttgaatatat caagcgttgg ggcaacatag tgatgactaa
      121 tgaaacaacg acgatagaca acaagcgatc aaggtagtct gtacccgcct gtaccaagat
      181 cacacttgtt gtctgactta aacctaaccc atgtaatagc tgaacgatca tactggatcc
      241 tgaggaggtg atcccgccaa ataactgaac ggtaatgatc gaagaaacga tcgttccagg
      301 taccgacaaa atcaaagcga gtaataacag tttcaagctg tttttatctt tgccgagata
      361 tcttggcaaa atcaatccag caagcaagcc tgtgagaatt tggattggcg aataatacag
      421 agaaaaaata tctgtagtca ctccacttaa aataccgctg gacaatcctg tcagcattcc
      481 aggtacaggc ccaagcaatg caccagcaaa aatcgttccg atagaatcga gatagattgg
      541 caaacgaagc cataaagcaa tcgttcctcc aatataattg atcccaatac acaaagccat
      601 cactgtaagt acgcgagttg ccattttgtt tttattcatt ttgttagccc atctttaacc
      661 tttccaaatc tttcataatc gtttcttctt gtgcatttaa aagaacg
//
LOCUS       DAZUKC010000238          643 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000238 DAZUKC010000000
VERSION     DAZUKC010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.255"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..643
                     /locus_tag="ACG80T_002904"
                     /pseudo
     CDS             1..643
                     /locus_tag="ACG80T_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612146.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          243 a          110 c          125 g          165 t
ORIGIN      
        1 gaattgttag aactacgtaa aagaaaccag caattagaaa tggaaaatga tattttaaag
       61 caagcagcgc tgatattcgg acgaagagac aagtaatcga tgcgaataag catctttacc
      121 ctatatcagc gatgtgcaga atattaggtc tatcacgtca gtcctattat tatcaatcaa
      181 aaccaaagaa agacgaatca gaacttgaag aagtagtcgc tgaagaattt atccgcagcc
      241 gaaaggccta cggctcaaga aaaataaaaa aagccttatc aaaacgaggc attcagatca
      301 gccgacgaaa aattagtaga atcatgaaaa atagaggatt aaaatcgagc tatactgttg
      361 cttattttaa agtacatcat tctacttgca atgaagccaa aacgacaaac gtattgaatc
      421 gtaaattctt aagagacaac ccattagaag cgatcgtaac agacttgact tatgtacgag
      481 tcgggaaaaa atggaattat gtctgtttca ttttggatct gttcaatcga gaaattctcg
      541 gctattcttg tggagaacat aaagatgccg ttctagtaaa aaaagcattt agccgtatca
      601 aacaacctct gacagaggtt gagatttttc atactgatcg tgg
//
LOCUS       DAZUKC010000239          640 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000239 DAZUKC010000000
VERSION     DAZUKC010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.256"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..283
                     /locus_tag="ACG80T_002905"
     CDS             <1..283
                     /locus_tag="ACG80T_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS4842477.1"
                     /translation="LIQDDLHHTHRLWLMNKINLYFGHSYVFAFYDYRTNITEVDLVI
                     SNYYIDTGKTPLLLMKNIPTERNWRLFEKTIYQLKKEKEVVKSAFCPEY"
BASE COUNT          218 a           89 c          119 g          214 t
ORIGIN      
        1 tttaatacaa gatgatttac accatacgca tcggttatgg ttaatgaata aaattaatct
       61 gtattttggg cattcttatg tttttgcttt ttatgattac aggacaaata taactgaagt
      121 agatttggtt atttctaatt actacatcga cactggaaaa acgccattac tgttaatgaa
      181 aaatatacct accgaaagaa actggcgttt gttcgagaaa acgatttatc aattgaaaaa
      241 agaaaaagag gttgtgaaat cagcgttttg tcctgagtat tagagaaaca aacggaaaaa
      301 tagtttttca tattttgccg tttttgagct aataccgagg gacagctgat tgaacaccat
      361 ttattgagag attgtgaaaa aactgtcctg catcaagtaa taagaacagc caagtaaaaa
      421 cagcttctca tgtttgttga atattgtgac ttgtgaccga ggagttggca agagaacacc
      481 gtttattcgg aaaaagggac tgtgacacta catgtcacag tccctttttg attgaattat
      541 ctcatttgtt ttctaatttt ttctttattt gagaaaatag ttgaactgga taaaaagtac
      601 tggacgtgtc aaaattattt tggatcaaat tagatctatt
//
LOCUS       DAZUKC010000240          630 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000240 DAZUKC010000000
VERSION     DAZUKC010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.257"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>630
                     /locus_tag="ACG80T_002906"
     rRNA            <1..>630
                     /locus_tag="ACG80T_002906"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          156 a          144 c          196 g          134 t
ORIGIN      
        1 gaaagccccc ggctcaaccg gggagggtca ttggaaactg ggagacttga gtgcagaaga
       61 ggagagtgga attccatgtg tagcggtgaa atgcgtagat atatggagga acaccagtgg
      121 cgaaggcggc tctctggtct gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag
      181 gattagatac cctggtagtc cacgccgtaa acgatgagtg ctaagtgttg gagggtttcc
      241 gcccttcagt gctgcagcta acgcattaag cactccgcct ggggagtacg accgcaaggt
      301 tgaaactcaa aggaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga
      361 agcaacgcga agaaccttac caggtcttga catcctttga ccactctaga gatagagctt
      421 ccccttcggg ggcaaagtga caggtggtgc atggttgtcg tcagctcgtg tcgtgagatg
      481 ttgggttaag tcccgcaacg agcgcaaccc ttattgttag ttgccatcat tcagttgggc
      541 actctagcaa gactgccggt gacaaaccgg aggaaggtgg ggatgacgtc aaatcatcat
      601 gccccttatg acctgggcta cacacgtgct
//
LOCUS       DAZUKC010000241          603 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000241 DAZUKC010000000
VERSION     DAZUKC010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.258"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(128..505)
                     /locus_tag="ACG80T_002907"
     CDS             complement(128..505)
                     /locus_tag="ACG80T_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842478.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
BASE COUNT          182 a          104 c          108 g          209 t
ORIGIN      
        1 aaagaagcag atccgttagc ggttctgctt cttttagttt attatatttt cttatcaata
       61 ataggaacct gattttaaaa gcggctacct ctttaccgtg cctctttaca tacaaaaaat
      121 agaggaatta agcgtaaatg gcttggctat tataactaaa atgctggtta tggagatgtt
      181 gcagcaagtt ttcgagaaaa gcgtattcca tctcttgtcc attgaataag atctggatac
      241 gataatgatt tttgaaacgc ttctgttcat gaatctgact gattgaaagt tcgcctttgt
      301 gttctgtctg ataaagaaaa gtccttttac ggcattcagc acagcgcttg gttgctcggc
      361 ggatttttcg gaatgtataa aaactagtat ctttattgca taaataaacg tctttgatcg
      421 acagcaaatc attatataga caggaacgtt cttcagctgt taattctgat aaaaaaagct
      481 gaccttgttt tattgcatct tccatcggca agtgcctcct cgaacccatt atccaaattt
      541 ttagttaatt ctaatatacc atcattaagt ataaatgtaa acgcttgttg atggtataag
      601 att
//
LOCUS       DAZUKC010000242          601 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000242 DAZUKC010000000
VERSION     DAZUKC010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.259"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..>601)
                     /locus_tag="ACG80T_002908"
     CDS             complement(27..>601)
                     /locus_tag="ACG80T_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFS4842479.1"
                     /translation="MTYLTQQFPLNHLENKIPIYFYFLVLAVVFFELLGESSSSFSTL
                     CFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQHLLQTETKEQLLI
                     HLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHEKISDETETFYLDT
                     THNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT          184 a           94 c          106 g          217 t
ORIGIN      
        1 aataagagga tgtgacaaga attttgtcac atcctctttg ttttgttctc ataacctttt
       61 ttcatataca gttcatgaac cgcttttatt aacttgccaa ataacactgt attgtgcgtt
      121 gtatccagat agaaagtctc cgtttcatct gaaattttct catgctgatc tgtaatgatc
      181 aaatcagcat gttctgaata tgcggtaatt ttaacagcat ctttgcgaaa taactgctgc
      241 aaatggcttt gaagatgata atttcctgta aactgtttgg ttatctgcaa atggatgagt
      301 aactgctctt tcgtttctgt ttgtagtaag tgttggatca attgactaag gtattgactg
      361 atttggtttc tttccccatt ttttgaaaaa attgtatcga ttttttctct taatttcttt
      421 tcctgtataa aagaactaga atgattggac gtggcttctg gaaagcaaag tgtagaaaaa
      481 ctactgctgg attcacccaa cagttcgaaa aaaacaactg ccaacacaag aaaataaaaa
      541 taaattggta ttttattttc taaatggttg agaggaaact gttgagttaa gtaagtcatc
      601 a
//
LOCUS       DAZUKC010000243          593 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000243 DAZUKC010000000
VERSION     DAZUKC010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.260"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>593)
                     /gene="wzy"
                     /locus_tag="ACG80T_002909"
     CDS             complement(<1..>593)
                     /gene="wzy"
                     /locus_tag="ACG80T_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy"
                     /protein_id="HFS4842480.1"
                     /translation="IKWLILAVIGIYILNVITKARDGGGLSKLTLKDFTSVFDSDDSP
                     LVDSFFEMGGTMSIITFFLISGNGIYPFANTYLTSLLGSISTRMLSMFGIKFVLLADW
                     FSQDYLGITWGTGFSMIAEAYVNGGYIGGIVYIFIIGIVIGKILSNSIDNQDIENHPM
                     KMFLAITTANIIMGFPRAASYLIVKNFVYGVLIILLI"
BASE COUNT          230 a          112 c           71 g          180 t
ORIGIN      
        1 tttatcaaca atataattaa aacaccatac acaaaatttt tcacaattaa atatgaagca
       61 gctctaggaa aacccattat aatatttgct gtagttatag ccaaaaacat cttcatagga
      121 tgattttcaa tatcttggtt gtctatacta ttagaaagga ttttaccgat gactatacca
      181 ataataaaaa tatagactat acccccaatg tatccaccat tgacataggc ttctgcaatc
      241 attgagaatc cagtccccca agtgattcct aaataatcct gagaaaacca atcagctaat
      301 aaaacaaact ttattccaaa cattgatagc attcttgttg atatagagcc taagagtgag
      361 gtcaaataag tattagcaaa gggatatatt ccattaccac ttattaggaa aaaagttata
      421 atactcatag tcccccccat ttcaaaaaaa gaatcaacta aaggagaatc atctgaatca
      481 aatacagatg taaaatcttt taaagtaagt ttacttagtc ctccaccatc acgtgctttc
      541 gttattacgt ttagaatata tattcctata acagctaaaa taagccactt tat
//
LOCUS       DAZUKC010000244          588 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000244 DAZUKC010000000
VERSION     DAZUKC010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.261"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(65..150)
                     /locus_tag="ACG80T_002910"
     tRNA            complement(65..150)
                     /locus_tag="ACG80T_002910"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Leu,seq:taa)
     gene            complement(167..238)
                     /locus_tag="ACG80T_002911"
     tRNA            complement(167..238)
                     /locus_tag="ACG80T_002911"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(204..206),aa:Gly,seq:gcc)
     gene            complement(250..322)
                     /locus_tag="ACG80T_002912"
     tRNA            complement(250..322)
                     /locus_tag="ACG80T_002912"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Thr,seq:tgt)
     gene            complement(340..421)
                     /locus_tag="ACG80T_002913"
     tRNA            complement(340..421)
                     /locus_tag="ACG80T_002913"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(385..387),aa:Leu,seq:tag)
     gene            complement(429..501)
                     /locus_tag="ACG80T_002914"
     tRNA            complement(429..501)
                     /locus_tag="ACG80T_002914"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(466..468),aa:Lys,seq:ttt)
     gene            complement(505..577)
                     /locus_tag="ACG80T_002915"
     tRNA            complement(505..577)
                     /locus_tag="ACG80T_002915"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(542..544),aa:Val,seq:tac)
BASE COUNT          149 a          169 c          138 g          132 t
ORIGIN      
        1 cccctaacct tttgatccgt agtcaaacac tctatccagt tgagctatgg gcgcatattt
       61 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
      121 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      181 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      241 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      301 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      361 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      421 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      481 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      541 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DAZUKC010000245          573 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000245 DAZUKC010000000
VERSION     DAZUKC010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.263"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..212
                     /locus_tag="ACG80T_002916"
     CDS             <1..212
                     /locus_tag="ACG80T_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS4842481.1"
                     /translation="NVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIGK
                     VLGITRQGATNLKHRLYKKIQKMLV"
     gene            306..497
                     /locus_tag="ACG80T_002917"
     CDS             306..497
                     /locus_tag="ACG80T_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4842482.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
BASE COUNT          215 a           79 c           94 g          185 t
ORIGIN      
        1 ttaatgtttt aaatgtgact ctccttgttg aagatataaa attagcaaaa atccttcctt
       61 tgttgaaaga acctgaacaa atttttctta tcgaaaaatt tatctttgat aaaactgata
      121 aagaaattgg aaaggttctg gggattacaa gacagggtgc gacaaattta aaacatcgac
      181 tttacaaaaa aattcaaaaa atgctggttt aatatctgca taataactaa ttttgtatgc
      241 ttctttgctc gttctcctcc ttcccaaaga aatagattat aagtccatgg gaagaaggta
      301 gatttatgaa ttctttaaaa gaagatttca ttttagcaaa ggctggaaat gaagaagctg
      361 ttgaggcaat tctcaaaaga tttagctctt tgattcataa gcaatcctgg agaactggaa
      421 aatatgatca agattgttat caagaatgta tgcttgcaat atatctagct atctcgaaat
      481 tcgaaattaa agaataactc aactaaggga gtctgtgaca taagtaaaaa gatagtttca
      541 aatttcgaac aataaatttg aaactatctt ctt
//
LOCUS       DAZUKC010000246          540 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000246 DAZUKC010000000
VERSION     DAZUKC010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.264"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          180 a          100 c           61 g          199 t
ORIGIN      
        1 tattatgcct tttttaatat ataacacagt tatttttaga aataagaatg ctaaatcact
       61 ctacaataac ggcgaaagca caaaaaataa aaaaattatt acgttattaa aaaaacagat
      121 gaaagatcag gttttattag atctcattct ttcatcactt gaagggattt aatgaactac
      181 tttttcctct ttgtcgtttt tatatattcg tattccttca aacagtagga tagcctcttt
      241 caatgcttga tatttgaact gtccgttact taaaaattta gatgtgccgc cctcttccta
      301 gaacatattc ttttttactt tttcattaat tgacagacca atcgaacgaa actgtaaatt
      361 tcgataggat aatgattggt caaactcatt cattttcaag acatctcgat tcatattcaa
      421 gataggaatc tttttatcaa catcacttta ttcgtatttt cctctaccat aaaagcttca
      481 cgctccacaa aattgtttta tacactacta ttcagcccat tcaacgacca atgaattttt
//
LOCUS       DAZUKC010000247          522 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000247 DAZUKC010000000
VERSION     DAZUKC010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.265"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(66..464)
                     /gene="spxA"
                     /locus_tag="ACG80T_002918"
     CDS             complement(66..464)
                     /gene="spxA"
                     /locus_tag="ACG80T_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFS4842483.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          154 a          106 c           89 g          173 t
ORIGIN      
        1 aagatttctc tttaccaaca tcatttgaca aagagccagt cttttccaag tttcgtatta
       61 tccttctata atccagccat caattgtgct tgacgtaatt ctaattgtcg tacatcgcgc
      121 ggtaggaacc gacgaatttc atcttcatta aaaccaactt gcagacgttt atcatcaatc
      181 atgattggac ggcgaagcaa tccaggattg ttttgcacaa gttccaataa ttcttgtaac
      241 ggcagatcat ctaaatccat gttcagtttt tggaatactt tggaacgtgt cgagatgatt
      301 tcttctgttc cgtcttctgt catttgtaaa atcgctttta attcagaact gttcaatggt
      361 tctgaaaaaa tattacgttc aacaaatgga atctgatgtt cttgcaacca cgcacgagcc
      421 tttctgcagg aagtacaact tggggaagta tataatgtta acaacgtgca ccactccttt
      481 tcgtgataag aatcactttt ttgtaacaga agatcataat aa
//
LOCUS       DAZUKC010000248          519 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000248 DAZUKC010000000
VERSION     DAZUKC010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.266"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(113..238)
                     /locus_tag="ACG80T_002919"
     CDS             complement(113..238)
                     /locus_tag="ACG80T_002919"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842484.1"
                     /translation="MHLHRISALAVQIGRYHHSETRAVMVTRCCLLALVIAAANA"
BASE COUNT          170 a           87 c          117 g          145 t
ORIGIN      
        1 tatccataaa actaatataa agcttggtac gattctgttt gtaagacata cgaatagtat
       61 cagaggtagg agccaagctg cctgatatta aattatcgat aaagtagaaa ggttaagcat
      121 tagccgctgc aataactaat gctaggagac agcaacgtgt aaccattacc gcacgtgttt
      181 ctgaatggtg gtagcgaccg atttgaacgg ctagggcgga aatacggtgc aaatgcacgg
      241 tggtttcatg caatgcttac agggcgtatt ttgtaagggt atgtaacgga gataattccg
      301 acggtaatgg atgtgagagg ccaagccgac gctgacggta gggcaaacaa acatagttgc
      361 actctagcga tcgttattta tttttaacgg tcaaactagg gtacaactat gcttaaaaca
      421 cttcgcctca acttctggta gcaaaagtga ttttgaaagt caaaatattt tttatgagtt
      481 atttacaatt ctaaaaaaca agtgtattaa tctactaat
//
LOCUS       DAZUKC010000249          514 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000249 DAZUKC010000000
VERSION     DAZUKC010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.267"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACG80T_002920"
     CDS             complement(<1..132)
                     /locus_tag="ACG80T_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFS4842485.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DAZUKC010000250          494 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000250 DAZUKC010000000
VERSION     DAZUKC010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.269"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..460
                     /locus_tag="ACG80T_002921"
     CDS             <1..460
                     /locus_tag="ACG80T_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4842486.1"
                     /translation="LKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSIS
                     DELRIAYFYYQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLAL
                     ELPYSNAKIENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          163 a           99 c           88 g          144 t
ORIGIN      
        1 attaaaggcg ttgtctcggc ttctgatcaa acgacaggac aagcttgttt atgacaaatg
       61 gaccaaatgg cgcaattttg gttgggcgta tctcaccgaa agtgaagtgg tggatcgtct
      121 gctttcgatc tctgacgaat tacgaatcgc ctatttttac tatcaagaaa ttcttcaagc
      181 gtttcacgac aaagaggcgg acactttctt taaacttgta agaacgatgc ccaattccgt
      241 tccaaaagaa ctgcattcta taaaaaaagc ttttataaac tatgaatccg gcattcgttt
      301 agccctcgaa ttgccttatt caaatgccaa aatagaaaat ctgcataccc acatcaaagc
      361 attgaaacga gtcgcttatg gctttcgaag ctttcggaaa atgaagacca gaattttctt
      421 actaaataat ctgattacct atgaatcaaa aaacatctaa agtagaaaag gaattctacc
      481 ttagatgttc ttat
//
LOCUS       DAZUKC010000251          490 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000251 DAZUKC010000000
VERSION     DAZUKC010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.270"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>490)
                     /locus_tag="ACG80T_002922"
     CDS             complement(<1..>490)
                     /locus_tag="ACG80T_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HFS4842487.1"
                     /translation="SLIFFSSILLLTLMNPFISLWVGAKYMLPMSTTILIVLNYSVQV
                     YRNTSFVFIESFGLYWSQRKKPIIEAGVNLLLSLLLLIVFDMGINGVLIGTIGSSLGF
                     VIWYEAFIIYKTVFEKRFIHFFLTFWRSFIELIISAIVVFLLNEYLANGGSTFLQFIT
                     KT"
BASE COUNT          200 a           82 c           65 g          143 t
ORIGIN      
        1 aaggttttag ttataaattg aaggaacgta ctgcctccat ttgctaaata ttcatttaat
       61 agaaaaacga ctattgccga aattattaat tctataaaac ttcgccagaa agttaaaaag
      121 aaatgaataa aacgtttctc aaataccgtt ttatatatga taaatgcttc ataccaaatc
      181 acaaatccta gagacgatcc gatagtccct ataagtactc cattgattcc catatcaaaa
      241 actattaaca aaagcaaaga taataacaag tttacacctg cttcaattat aggctttttc
      301 cgttgagacc aatataaacc aaatgactct ataaaaacga aggatgtatt tctataaacc
      361 tgtacactat aatttaaaac aattaaaata gtcgttgaca taggtagcat gtatttggct
      421 cctacccaca agctaatgaa gggattcatt aaagtcaata acaatataga agaaaaaaag
      481 attagtgagt
//
LOCUS       DAZUKC010000252          476 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000252 DAZUKC010000000
VERSION     DAZUKC010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.273"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            25..>476
                     /locus_tag="ACG80T_002923"
     CDS             25..>476
                     /locus_tag="ACG80T_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4842488.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSDTFSLDKDYELLNPIIKNIFTKYYSKDFEIYLNEILANNINPL"
BASE COUNT          197 a           75 c           71 g          133 t
ORIGIN      
        1 ttaaaaataa aaaggagaaa aaagatgaag ccaaatcctt cagaagcagg aaaacgaatc
       61 aaacaattac gcctttcatg tggttttact atggaagaac ttggaaggaa aatagataat
      121 tcaccacgtg cgaccatatc caactgggaa cgtgggacca atttaccaaa tccacaaaag
      181 ctgaaattat tatcaactat cactaattca acaattgact ggattaaatg gggaacttta
      241 gaagaatata taacttcata ccttatcgat atagggtatg agctatatat taaggacttc
      301 ccagaaatac ctcacaaagt ctttaaagac attcaagaaa gatatagtga tacgttttcg
      361 ctagataaag actatgagtt attgaatcct ataataaaaa atatatttac aaaatattac
      421 tcaaaagatt ttgaaattta tctaaatgag atacttgcta ataatatcaa tcctct
//
LOCUS       DAZUKC010000253          470 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000253 DAZUKC010000000
VERSION     DAZUKC010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.274"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            185..409
                     /locus_tag="ACG80T_002924"
                     /pseudo
     CDS             185..409
                     /locus_tag="ACG80T_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          162 a           66 c           76 g          166 t
ORIGIN      
        1 tttctaacaa aagcacatat tttaatatat actacctatt ttaggtagta tatattttta
       61 gatacaatat cgatcagttt tgaggaaatg aaattctaga tattttttgc tacatagagt
      121 tactttcaat attttttaac gtaattaggc agaagtcact catcctctat aggtttgtga
      181 tgaaattgcc attgatccat ttaagtaaga gcaaaaaact ggaggtttat caggtaattg
      241 gtctaatgga tttaagtatg cggtcaccgt gtatagagtt gcttatgaga ttcaagaaaa
      301 taaagttatt ccagtattgc tatatggaac acataacaat ttttatgaat tagccgagaa
      361 cactcgcaac tttattcgtg tagatgaatc ggcttcggtt gagttataac tttagctata
      421 tcaatcaacc gaccgacata tgttaaatct atatataata aaaataccta
//
LOCUS       DAZUKC010000254          465 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000254 DAZUKC010000000
VERSION     DAZUKC010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.275"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>465
                     /gene="bsh"
                     /locus_tag="ACG80T_002925"
     CDS             <1..>465
                     /gene="bsh"
                     /locus_tag="ACG80T_002925"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile salt hydrolase"
                     /protein_id="HFS4842489.1"
                     /translation="ADKEKSIVIESMKDGLHIYDNPVGVLTNNPSFDYQLFNLNNYRV
                     LSSETPKNNFSNQISLNAYSRGMGGIGLPGDLSSVSRFVKATFTKLNSVSGDSESESI
                     SQFFHILGSVEQQKGLCDVGDGKYEYTIYSSCCNVDKGIYYYRTYEDSQITAI"
BASE COUNT          155 a           66 c           85 g          159 t
ORIGIN      
        1 gctgataaag aaaaatcaat tgtcattgaa agtatgaaag atggacttca tatatatgat
       61 aaccctgtgg gcgttcttac caataatcct tcatttgact atcaattatt taatttaaac
      121 aattatcgtg tcttatcgag tgaaactcct aaaaataatt tttcaaatca aataagtttg
      181 aatgcctata gccgcggtat gggagggata ggcttgcctg gagatttatc ctcagtatct
      241 cgttttgtta aagcgacttt tacgaagctg aattctgtat ctggagattc agagtcagaa
      301 agtattagtc aatttttcca tatcttaggt tcagtagaac aacaaaaagg tttgtgtgat
      361 gttggtgatg gaaaatatga atatacaatt tattcttctt gttgcaatgt tgacaaagga
      421 atctattatt atcgaacata tgaagacagt caaattactg caatt
//
LOCUS       DAZUKC010000255          460 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000255 DAZUKC010000000
VERSION     DAZUKC010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.277"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(20..91)
                     /locus_tag="ACG80T_002926"
     tRNA            complement(20..91)
                     /locus_tag="ACG80T_002926"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="ACG80T_002927"
     tRNA            complement(113..200)
                     /locus_tag="ACG80T_002927"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="ACG80T_002928"
     tRNA            complement(225..298)
                     /locus_tag="ACG80T_002928"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="ACG80T_002929"
     tRNA            complement(312..382)
                     /locus_tag="ACG80T_002929"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="ACG80T_002930"
     tRNA            complement(384..459)
                     /locus_tag="ACG80T_002930"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
BASE COUNT          132 a          128 c          108 g           92 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca
//
LOCUS       DAZUKC010000256          451 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000256 DAZUKC010000000
VERSION     DAZUKC010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.278"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>451
                     /locus_tag="ACG80T_002931"
     CDS             <1..>451
                     /locus_tag="ACG80T_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFS4842490.1"
                     /translation="ESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPITA
                     DDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKE
                     KVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          182 a           67 c          105 g           97 t
ORIGIN      
        1 ttgaaagtgg aacaacagtc tttgcagtga aagacggcgt aagcttacca gaagataaac
       61 ttccagtgtt gaaagcaaaa gatggttata cagatgcgaa atggccagag gaagcaactc
      121 aaccaattac agcagatgat acagaatttg tatcaagtgc aacaaaactg gatgataaat
      181 ctgacgctga caaatataat cctgaaggtc aaaaagtgac tacagaattg aataaggaac
      241 cagacgcatt tgagggaatt aagaacaaag aagatttacc aaaagatact aagtatactt
      301 ggaaagaaaa agtagatgtt agcgcagctg gaaataaaaa agggactgtt gtagtgacat
      361 atccagatgg atcatctgat gaagttgaag tagatgttac agtgacagac aatcgttctg
      421 acgccgataa atatgagcca acagtagaag g
//
LOCUS       DAZUKC010000257          449 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000257 DAZUKC010000000
VERSION     DAZUKC010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.279"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..>449)
                     /locus_tag="ACG80T_002932"
     CDS             complement(38..>449)
                     /locus_tag="ACG80T_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842491.1"
                     /translation="KELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIIDFSYGAAYN
                     QFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDPPRAVDWGN
                     LTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          137 a           82 c           74 g          156 t
ORIGIN      
        1 tttagaaaaa gcagtcggac tttctaaaag aagttcacta ttcgatcctg tcgatgattt
       61 cttcaggaaa atcgtctatt tgcttgtcaa ttttgctgtc tttgtatttc tcttcttgga
      121 tagtcaacaa aatctttgta agattccccc agtctactgc tcttggcgga tcgagccaaa
      181 ccactacttg agatgcactt tcttcatata gattattcct attcgtgatt ataacatccg
      241 ggaactcagg ctttcctgcc tctatcactt ccgtgttcag attaatgtac aagcttaaat
      301 ttttcttgat aaattgatta taggcagcac cataagaaaa atcgatgatg atctttactg
      361 gttcagaaat cagttgaatg ggtatgtgat tgatcaataa taataaataa ctgaaaaaaa
      421 gaaaaaattt atcttgaagc aactctttg
//
LOCUS       DAZUKC010000258          430 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000258 DAZUKC010000000
VERSION     DAZUKC010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.281"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>430)
                     /locus_tag="ACG80T_002933"
     CDS             complement(<1..>430)
                     /locus_tag="ACG80T_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842492.1"
                     /translation="NQKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEH
                     IIKHEAKIGSQWVNLPYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQ
                     VNANGLVQISGVKGVADIKVSTSYTVTTIISYLVTLTAYL"
BASE COUNT          126 a           79 c           63 g          162 t
ORIGIN      
        1 cataaatatg cagttaatgt aacaagataa gaaataatag tcgttactgt ataacttgtg
       61 cttactttaa tatcagcaac tcctttgact ccgcttattt gtaccaatcc attagcattc
      121 acctgctcct ttacaggttg atgatttatt tctacattcg ttcctaaata agcatataca
      181 ggaagtgtga ttacttggct cttctcatta gttactctat atatgataga actaccagtt
      241 gttttataag gaaggtttac ccattgactg ccaatctttg cttcgtgttt tattatatgc
      301 tccttattct ccaatgaagc gataggagaa taatcatata aaaaataatc cattccttta
      361 gtggtatacg cctctactgt ttctttgttt acaacaatat gtcccggctg agaaattatt
      421 ttttgattga
//
LOCUS       DAZUKC010000259          429 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000259 DAZUKC010000000
VERSION     DAZUKC010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.282"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          122 a           75 c           57 g          175 t
ORIGIN      
        1 ttgtgcatta tttcttcaca tttattaggt tatagtttaa gaaagtttaa acattgcgca
       61 atttaattat tttagctgtt tcttttcata aaaaaacatg ttgtgactct gagacaaatc
      121 atagtccata gtgatgtctc tagagtaact acgcctaata ttacgaggca tcatattaat
      181 ctatcagcac tagtacctaa actagcatcc ctttcgaata ttttttctta tataataaag
      241 tttgctttca ctcctctaat ctcaccaaat cgactggctt gcgttgtgtg atactaggaa
      301 ctgattggtt tagaacgact tttctattgt aatcatattt ttcgccatcc tcgttgtctt
      361 ctaatccttc aatgaatgat ccctaatgtg tgtattaaat aatttaattt ttttgttcct
      421 atttattta
//
LOCUS       DAZUKC010000260          419 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000260 DAZUKC010000000
VERSION     DAZUKC010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.283"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>419
                     /locus_tag="ACG80T_002934"
     CDS             <1..>419
                     /locus_tag="ACG80T_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFS4842493.1"
                     /translation="KIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNL
                     YLDSTHIKANANKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKL
                     KERKISKADPESGYYVKGEREKQFAYSAHTSCDDNGF"
BASE COUNT          154 a           72 c           87 g          106 t
ORIGIN      
        1 aaaatccccc atttctcaac ttttggaaag aattatgttc gccgatttag agaaacgacg
       61 gtgtttgagg atatttttgc ctatatttta gaacaagcag ttaaagcagg ttttgtaaca
      121 gaggataatt tgtatctgga ttctacccac atcaaagcta acgccaataa acataagttt
      181 acgaaagaaa tgacccatga tgaagcgaaa gcctatcagg atgaattaga agatgagatc
      241 aatcgtcaac gaatagaggc tggaaaacgc ccttttactt tggacttaga aaaggaagtg
      301 aagctgaaag aaagaaaaat aagtaaagct gatccggaaa gcggctacta cgtcaaaggc
      361 gaacgagaaa aacaatttgc ttattctgcc catacttcat gcgacgacaa tggatttat
//
LOCUS       DAZUKC010000261          418 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000261 DAZUKC010000000
VERSION     DAZUKC010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.284"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            255..>418
                     /locus_tag="ACG80T_002935"
     CDS             255..>418
                     /locus_tag="ACG80T_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS4842494.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIK"
BASE COUNT          151 a           70 c           60 g          137 t
ORIGIN      
        1 gtaaaaagct caagacattt tcttagccat aaggacatgt ttgatattta agaagatata
       61 tgaattttct tttctaagta taaagataac tatccattat caactaaaaa aacatttttg
      121 aaaataaaca tcagcagtcc tatcaatgca tgtgagctta tagaagttat tcgcagcgca
      181 gcataattgc attagcttct tttcgtgttt tttgcctctt ttccctttaa atcttaatgg
      241 aggaggtgtg caatatgtca gataacgacg aacgcgtaaa tgcccagttg attcgattct
      301 ttgaaaaggt cattttgaac accgctaata cttattataa aaaacaagaa gaaattcaaa
      361 aacatgaaca acttgatcaa ctcccaccat atttgtttac taaagatact ataaaatt
//
LOCUS       DAZUKC010000262          401 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000262 DAZUKC010000000
VERSION     DAZUKC010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.285"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>401
                     /locus_tag="ACG80T_002936"
     CDS             <1..>401
                     /locus_tag="ACG80T_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFS4842495.1"
                     /translation="VLFPNGDFPSRSRFTRLSSNLAYTIKIIRYFFIKKLTKGELVGI
                     IDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKTGFPITYSITN
                     PGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          137 a           75 c           66 g          123 t
ORIGIN      
        1 cgtcttattt cctaatggtg actttcctag taggagtcga ttcactcgct taagttcaaa
       61 cttagcttac actatcaaga ttattcgata ctttttcatc aagaagctca ccaaaggtga
      121 actagtcgga attatagata gttttcctag tccattatgt aaaccagtta gaaatagaca
      181 agcaaaacta ttgaatcaaa tagccaaagt tggctataac tcaacaaaaa aatcttattt
      241 ttatggtctt aaaattcata tgatcgttac taagactggc tttccgatta cttactcaat
      301 cacgaatcca ggtgtccatg acgtgaaagt gttagagact ttatcggaag aagcgaatct
      361 tcccaatatc ctaggggaca aaggatatat tagccataaa a
//
LOCUS       DAZUKC010000263          390 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000263 DAZUKC010000000
VERSION     DAZUKC010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.287"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            136..327
                     /locus_tag="ACG80T_002937"
                     /pseudo
     CDS             136..327
                     /locus_tag="ACG80T_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT          166 a           47 c           60 g          117 t
ORIGIN      
        1 aaacaggata tatagaatca aaatacgaat cttattttga tttttctagt tttttgaaaa
       61 aagcaccctt tgatcgttgt attagtggaa aagagaaaaa ggagcatact tatcatgtca
      121 caaaactaca actaaatatc taaacaacac caatttgatg cctattgaaa aaaagtattg
      181 cgaaatgaag ccaagtctat tagaaaatga aataatagaa taaaagaaaa tgggaaaccg
      241 ctgaatgatg taaatgaagg taagtatcca cgaactaatt ttgatgaaca agatatctat
      301 tttttatttg tattggagat actagtttaa tatcaaaagt tatcagcagt cataattagt
      361 tatccaatgt aaaaagtaca aacaatagtt
//
LOCUS       DAZUKC010000264          382 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000264 DAZUKC010000000
VERSION     DAZUKC010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.290"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>382
                     /locus_tag="ACG80T_002938"
     CDS             <1..>382
                     /locus_tag="ACG80T_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFS4842496.1"
                     /translation="FDITQTQLKPEDYPKIFPNRVFDFQLDKKGQSELQAGIQAVANN
                     IGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNTRNTPTQHLATSIHELAHA
                     RMHKFSELDTATKELQAEMTSYIV"
BASE COUNT          140 a           68 c           76 g           98 t
ORIGIN      
        1 tgtttgatat cacacaaaca cagttaaaac ctgaagacta tccgaaaatc tttccgaacc
       61 gtgtctttga ttttcaattg gataaaaaag gacagtcaga acttcaagct gggattcaag
      121 cggtagccaa caatattgga atacaaattc gagatatgac agaaagtgaa gtctatcaaa
      181 gagaattagg acaagcacgt ggggcgtatg ttcgtaacga atttactggc gaagaagaaa
      241 tcgtcatgaa tactcgaaat actcctacgc agcatttagc tacaagtatt catgaattag
      301 cccatgcaag aatgcacaaa ttttcagaat tagataccgc aactaaagaa ttgcaggcag
      361 aaatgacttc ttatattgtg tg
//
LOCUS       DAZUKC010000265          381 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000265 DAZUKC010000000
VERSION     DAZUKC010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.291"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(21..94)
                     /locus_tag="ACG80T_002939"
     tRNA            complement(21..94)
                     /locus_tag="ACG80T_002939"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="ACG80T_002940"
     tRNA            complement(108..181)
                     /locus_tag="ACG80T_002940"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="ACG80T_002941"
     tRNA            complement(209..281)
                     /locus_tag="ACG80T_002941"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="ACG80T_002942"
     tRNA            complement(301..374)
                     /locus_tag="ACG80T_002942"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
BASE COUNT          103 a          108 c           90 g           80 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgtttaat t
//
LOCUS       DAZUKC010000266          380 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000266 DAZUKC010000000
VERSION     DAZUKC010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.292"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(143..>380)
                     /locus_tag="ACG80T_002943"
     CDS             complement(143..>380)
                     /locus_tag="ACG80T_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4842497.1"
                     /translation="PRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVK
                     GLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT          110 a           65 c           56 g          149 t
ORIGIN      
        1 taaagtaaca atctttcagc tgagtgttag gttctgttac taaatatata gaattttcca
       61 tatccaccct cctatgttag atttggataa tttttgtttt attatgttta tattattaca
      121 taacccgtgg tgctagttaa ttttaatatc ctaaagaata tctcaaagtt ccttcaaaac
      181 gtcgttgtgc tcgacttaat agaacactgt aagccagtaa tatgctttca aatctactct
      241 ctagcccttt aacagaacgt gagttgaaat tttgacaacc taatttttct aaggaagaaa
      301 aaacagtctc aactgttttt ctttgttttc caagtaagct gtggtctagt ttttctgatt
      361 tatccatgtt tttacgtggc
//
LOCUS       DAZUKC010000267          366 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000267 DAZUKC010000000
VERSION     DAZUKC010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.293"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          151 a           49 c           53 g          113 t
ORIGIN      
        1 ttttaatacc tataccaatt taaagaggat ttttcagatt ttcacttaca aatttataaa
       61 aaaacaatat aattgatact ttgattttgc gaacattttg cctttccgcc taattcagct
      121 ttaatacctt gattatatca gtaactaatc gcctttttag ctaatttaag catcaaataa
      181 ttatccttaa tcagggtaaa aaataacgaa taaatggatt tttggaatag gtaaaaacta
      241 gatcggtttc tgacgaaaaa taacattaaa aaggaaatat aaaataacat ggagcgtggg
      301 aggaataaag aaattttaca cacaaggggc gtaggaatgt aaagaaactt aacacaaaac
      361 aaaagt
//
LOCUS       DAZUKC010000268          343 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000268 DAZUKC010000000
VERSION     DAZUKC010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.295"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          138 a           42 c           43 g          120 t
ORIGIN      
        1 tattcagaag taataatatc gctactatta agataaaatt actataaaaa agcaaaatat
       61 tacctcctaa ttaaatatta aataccgaaa aaattatgtt tagataatac catagttaag
      121 tttttccttg tgatgttttt cgtaaaaaga tattttagca gcattatcgt tacagtgcat
      181 agttaccatc ggcgttatga ctatataaag tatactttcg ttgatccaga aaggtatata
      241 gattaaaata agttacctaa aaaggacaaa tacaaatttt attatcttat tcacagtaag
      301 aagataattt taagattgcg aaatacgata ctatttgtaa tct
//
LOCUS       DAZUKC010000269          325 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000269 DAZUKC010000000
VERSION     DAZUKC010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.300"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          121 a           54 c           78 g           72 t
ORIGIN      
        1 tagaatgtat gtaaataaat aaaatgtctg acggatattg aatagcaata aataacaata
       61 attcagacgg atacagtgag tgaagtagct tgctacgaac gaacaggcag aatgaaacgg
      121 cagtttcatt agagcaagtc gcttgcgacg tagcgttacc gctgatgtaa ctggcaagtt
      181 acatagagag agtttgcttg caaacgaacg ttacctactt gaatagaaat aaaaaataac
      241 agattagtga attttgaata ggagccacaa ctcaaccaac cgcttgcggt gggtgagtcc
      301 cacggaggaa aagacgaacg aacgg
//
LOCUS       DAZUKC010000270          323 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000270 DAZUKC010000000
VERSION     DAZUKC010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.301"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            24..>323
                     /locus_tag="ACG80T_002944"
     CDS             24..>323
                     /locus_tag="ACG80T_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFS4842498.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKY"
BASE COUNT          118 a           34 c           60 g          111 t
ORIGIN      
        1 gaaatataag aaggagtata aaaatgaaca aacataatag aaaaataacg aataacaaag
       61 aactgatctt gttactaata gggatttggg gtatcttttt actctttaat atgtatatgc
      121 cgactatgag agcggatgac gtagtttatg caagtagatt agatgaactt ggctatttgg
      181 gtgcttcgat agaacattat aagacatgga gttcaagaat tattatagaa ctattcttaa
      241 tgttcttttc taaacacttt atgctatgga aattattaaa ttctacgatt atgttaggaa
      301 ttgtagtatt actttgcaag tat
//
LOCUS       DAZUKC010000271          321 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000271 DAZUKC010000000
VERSION     DAZUKC010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.302"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>321)
                     /locus_tag="ACG80T_002945"
     rRNA            complement(<1..>321)
                     /locus_tag="ACG80T_002945"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           63 a           96 c           74 g           88 t
ORIGIN      
        1 aaatccggac aacgcttgcc acctacgtat taccgcggct gctggcacgt agttagccgt
       61 ggctttctgg ttagataccg tcaagggatg aacagttact ctcatccttg ttcttctcta
      121 acaacagagt tttacgatcc gaaaaccttc ttcactcacg cggcgttgct cggtcagact
      181 ttcgtccatt gccgaagatt ccctactgct gcctcccgta ggagtttggg ccgtgtctca
      241 gtcccaatgt ggccgatcac cctctcaggt cggctatgca tcgtggcctt ggtgagccgt
      301 tacctcacca actagctaat g
//
LOCUS       DAZUKC010000272          309 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000272 DAZUKC010000000
VERSION     DAZUKC010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.304"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..309
                     /locus_tag="ACG80T_002946"
                     /pseudo
     CDS             1..309
                     /locus_tag="ACG80T_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          122 a           61 c           59 g           67 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcagc
      301 gatgtgcaa
//
LOCUS       DAZUKC010000273          303 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000273 DAZUKC010000000
VERSION     DAZUKC010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.305"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACG80T_002947"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACG80T_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFS4842499.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT           95 a           45 c           50 g          113 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atg
//
LOCUS       DAZUKC010000274          303 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000274 DAZUKC010000000
VERSION     DAZUKC010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.306"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>303
                     /locus_tag="ACG80T_002948"
     CDS             <1..>303
                     /locus_tag="ACG80T_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFS4842500.1"
                     /translation="DSTPVSIVPRKRIATRGGEILKGQIIKDRELIETTKKMLEKLNA
                     IGHITIQCIKNEKGIFYLEINARYGGGAPISIKSGANSPEYIIKMIDSKKNLRYRE"
BASE COUNT          132 a           35 c           60 g           76 t
ORIGIN      
        1 ttgattcgac accagtaagt attgttccta gaaaaagaat agcaactaga ggtggggaaa
       61 tactgaaggg tcagattatt aaagatagag aactaattga gactacgaaa aagatgttgg
      121 aaaaattaaa tgcaatcgga catattacta ttcaatgtat aaaaaacgaa aagggaatat
      181 tttatttaga aataaatgca agatacggtg gaggagcccc cattagcatt aaatcaggag
      241 ctaattcccc tgaatatatt ataaagatga tagattcaaa aaaaaatttg agatacagag
      301 aaa
//
LOCUS       DAZUKC010000275          299 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000275 DAZUKC010000000
VERSION     DAZUKC010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.307"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>299
                     /locus_tag="ACG80T_002949"
     CDS             <1..>299
                     /locus_tag="ACG80T_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS4842501.1"
                     /translation="TIVGIHHKSAVITLVERLSKAIIALKPEGRKAVDIENSINEWLQ
                     SVPKNLFKSITFDCGKEFSNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRK"
BASE COUNT          102 a           63 c           54 g           80 t
ORIGIN      
        1 acgattgtcg gcatccacca taaaagtgcc gtcatcacac tcgttgagcg actttcaaaa
       61 gccattatcg ccttgaaacc agaaggccgt aaggcagttg atattgaaaa ttcgattaat
      121 gaatggcttc aatccgtacc caaaaatctt ttcaaatcaa ttacctttga ttgtggaaaa
      181 gaattctcta attggaaaag tattagtaat acgaatgata ttgatattta tttcgcagac
      241 ccaggaacgc cttcccaacg gggattaaat gaacattcaa acggactcct ccgaaaaga
//
LOCUS       DAZUKC010000276          293 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000276 DAZUKC010000000
VERSION     DAZUKC010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.308"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..269
                     /locus_tag="ACG80T_002950"
     CDS             <1..269
                     /locus_tag="ACG80T_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842502.1"
                     /translation="NTTELTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKN
                     QKTVVYGISLLILSIIPFFLLTLLWVIPGVLGIIAGLMLLIRKS"
BASE COUNT           94 a           32 c           53 g          114 t
ORIGIN      
        1 taaatactac agaactaacc agtaaggata tagatatgtt agtcaatcta agcgggaatg
       61 ttgttttatt tggaggaatt accctagttt tttcgataat gattttcata ataggttttt
      121 tagtaagaaa aaatcaaaaa acagtggtct atggtattag tttactcatt ttatctatta
      181 ttccattttt tcttttaaca ttattgtggg taattcctgg agttttaggt attattgcgg
      241 gattgatgct tctaataaga aaatcttaat taggcaatgt gtagacaacg tga
//
LOCUS       DAZUKC010000277          268 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000277 DAZUKC010000000
VERSION     DAZUKC010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.denovo.309"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>268)
                     /locus_tag="ACG80T_002951"
     CDS             complement(<1..>268)
                     /locus_tag="ACG80T_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFS4842503.1"
                     /translation="DYEISYNEVVTITPRNYKLNFRKVNDLDTHYAMIGIAAGIADYP
                     LYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFIPWILG"
BASE COUNT           91 a           53 c           34 g           90 t
ORIGIN      
        1 tcctaaaatc caaggaataa attcaaaagg agatacattg tctttccctt cttgtatttc
       61 tttataatca gcatacccag aaaaatttag tccagccata ctcaatcctt tttcatttgt
      121 cgcatcgtaa taaagagggt agtcagctat accagcggca ataccaatca ttgcataatg
      181 agtatccaaa tcatttacct ttcgaaaatt caacttataa tttcttggag taatagtgac
      241 tacttcattg taagatattt cataatca
//
LOCUS       DAZUKC010000278        35462 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.guided.022, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000278 DAZUKC010000000
VERSION     DAZUKC010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.guided.022"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            449..1414
                     /locus_tag="ACG80T_002952"
     CDS             449..1414
                     /locus_tag="ACG80T_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6017 domain-containing protein"
                     /protein_id="HFS4842504.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            1436..1648
                     /locus_tag="ACG80T_002953"
     CDS             1436..1648
                     /locus_tag="ACG80T_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFS4842505.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            1723..2199
                     /locus_tag="ACG80T_002954"
     CDS             1723..2199
                     /locus_tag="ACG80T_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HFS4842506.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            2196..4004
                     /locus_tag="ACG80T_002955"
     CDS             2196..4004
                     /locus_tag="ACG80T_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFS4842507.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            4081..4296
                     /locus_tag="ACG80T_002956"
     CDS             4081..4296
                     /locus_tag="ACG80T_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family mobile element protein"
                     /protein_id="HFS4842508.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            4388..5251
                     /locus_tag="ACG80T_002957"
     CDS             4388..5251
                     /locus_tag="ACG80T_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB6/TrbL-like conjugal transfer protein,
                     CD1112 family"
                     /protein_id="HFS4842509.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            5288..5824
                     /locus_tag="ACG80T_002958"
     CDS             5288..5824
                     /locus_tag="ACG80T_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFS4842510.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            5838..6260
                     /locus_tag="ACG80T_002959"
     CDS             5838..6260
                     /locus_tag="ACG80T_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HFS4842511.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            6190..8589
                     /locus_tag="ACG80T_002960"
     CDS             6190..8589
                     /locus_tag="ACG80T_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HFS4842512.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            8621..10612
                     /locus_tag="ACG80T_002961"
     CDS             8621..10612
                     /locus_tag="ACG80T_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFS4842513.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            10635..10886
                     /locus_tag="ACG80T_002962"
     CDS             10635..10886
                     /locus_tag="ACG80T_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HFS4842514.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            10876..12105
                     /locus_tag="ACG80T_002963"
     CDS             10876..12105
                     /locus_tag="ACG80T_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1107 family mobile element protein"
                     /protein_id="HFS4842515.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            12102..14183
                     /locus_tag="ACG80T_002964"
     CDS             12102..14183
                     /locus_tag="ACG80T_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFS4842516.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            14332..18252
                     /locus_tag="ACG80T_002965"
     CDS             14332..18252
                     /locus_tag="ACG80T_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodL domain-containing protein"
                     /protein_id="HFS4842517.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLPNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            18253..19197
                     /locus_tag="ACG80T_002966"
     CDS             18253..19197
                     /locus_tag="ACG80T_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842518.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            19201..19404
                     /locus_tag="ACG80T_002967"
     CDS             19201..19404
                     /locus_tag="ACG80T_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HFS4842519.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(19704..19940)
                     /locus_tag="ACG80T_002968"
     CDS             complement(19704..19940)
                     /locus_tag="ACG80T_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4842520.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(20123..20494)
                     /locus_tag="ACG80T_002969"
     CDS             complement(20123..20494)
                     /locus_tag="ACG80T_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HFS4842521.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(20491..21819)
                     /locus_tag="ACG80T_002970"
     CDS             complement(20491..21819)
                     /locus_tag="ACG80T_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFS4842522.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(21780..22109)
                     /gene="mobC"
                     /locus_tag="ACG80T_002971"
     CDS             complement(21780..22109)
                     /gene="mobC"
                     /locus_tag="ACG80T_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFS4842523.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(22367..22738)
                     /locus_tag="ACG80T_002972"
     CDS             complement(22367..22738)
                     /locus_tag="ACG80T_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4842524.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            22883..23068
                     /locus_tag="ACG80T_002973"
     CDS             22883..23068
                     /locus_tag="ACG80T_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS4842525.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            23226..23393
                     /locus_tag="ACG80T_002974"
     CDS             23226..23393
                     /locus_tag="ACG80T_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842526.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            24011..24678
                     /gene="vanR-B"
                     /locus_tag="ACG80T_002975"
                     /pseudo
     CDS             24011..24678
                     /gene="vanR-B"
                     /locus_tag="ACG80T_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
     gene            24681..26024
                     /gene="vanS-B"
                     /locus_tag="ACG80T_002976"
     CDS             24681..26024
                     /gene="vanS-B"
                     /locus_tag="ACG80T_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFS4842527.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            26195..27001
                     /locus_tag="ACG80T_002977"
     CDS             26195..27001
                     /locus_tag="ACG80T_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HFS4842528.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            27019..27846
                     /gene="vanW-B"
                     /locus_tag="ACG80T_002978"
     CDS             27019..27846
                     /gene="vanW-B"
                     /locus_tag="ACG80T_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HFS4842529.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            27843..28814
                     /locus_tag="ACG80T_002979"
     CDS             27843..28814
                     /locus_tag="ACG80T_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFS4842530.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            28807..29835
                     /gene="vanB"
                     /locus_tag="ACG80T_002980"
     CDS             28807..29835
                     /gene="vanB"
                     /locus_tag="ACG80T_002980"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFS4842531.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            29841..30449
                     /locus_tag="ACG80T_002981"
     CDS             29841..30449
                     /locus_tag="ACG80T_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFS4842532.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(30701..31705)
                     /locus_tag="ACG80T_002982"
     CDS             complement(30701..31705)
                     /locus_tag="ACG80T_002982"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4842533.1"
                     /translation="MQRGGKSRGKAAAAGSGKQKASNGERFLKKIERTGGEAVNRLTR
                     RRKVYEAIHTLRQEKGYEIASLCALSGIPRCSYYKWLHREESASEKENTLLLEEIIRL
                     YSEVKGIYGYRRMTLNVNSRLKRNYNHKRIYRLMKSVHMQAVIRRKKKHYVILEPRVT
                     AENVLNREFTANGPNEKWLTDVTEFKLINGKKAYLSAILDLHDKSIVAYALGQSNNNQ
                     LVFDTFDRAVRANPGAHPLFHSDRGYQYTNRQFKAKLDGIHATQSMSRPGRCIDNGPM
                     EGFWGILKSEMYYLRQFHTFEELKKAIDEYIDFYNTRRLQAKLKGLAPLAYRNQTLVA
                     "
     gene            complement(31588..32271)
                     /locus_tag="ACG80T_002983"
     CDS             complement(31588..32271)
                     /locus_tag="ACG80T_002983"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4842534.1"
                     /translation="MSLKGKIPPEVKIQAVEDYLAIRKGSTQIREELGIRLSTFQAWL
                     RKYQTEGPAGLHPKKHVTSYPSSLKLAAVEDYMGGGGSLDAMCRKYGISSHAVLQQWI
                     TLYNQGHREFKTRRAREETDMAEKEKLSQEQKLQAVLYCLEHQLDYRRTSEQYKITYQ
                     QIYSWVKKYQEQGEAGLLDRRGRRRPPSEYSEEEKAAAKLRQLEAENRRLQMENDFLK
                     KLNELEGRR"
     gene            32717..33148
                     /locus_tag="ACG80T_002984"
     CDS             32717..33148
                     /locus_tag="ACG80T_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS4842535.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
     gene            33155..33388
                     /locus_tag="ACG80T_002985"
     CDS             33155..33388
                     /locus_tag="ACG80T_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4842536.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            33812..34012
                     /locus_tag="ACG80T_002986"
     CDS             33812..34012
                     /locus_tag="ACG80T_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFS4842537.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            34096..35289
                     /locus_tag="ACG80T_002987"
     CDS             34096..35289
                     /locus_tag="ACG80T_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4842538.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
BASE COUNT         8927 a         9090 c         9589 g         7856 t
ORIGIN      
        1 ggcatttcgc aggatatgag aactcaattt actaccaatt tactactttc ggattgagcc
       61 gcatatatgc tgaaccatac tgctaatacc cgccacccca aatacggcac atcataaatt
      121 gaatacgaca ccgtaaacgc ggcgttcctc tttcccgtca tgggagaaca ggacgccgct
      181 tttttatgcc caaatagaga aaggaggcga cgccatgcta tttacttccg gccctgaccg
      241 ggcgtttgaa cggctgatgc aggaaaagcc cggtttcgat cactacgacg gcggcggggc
      301 ggacgcgccg gaggattgcg gcacctgccg attttaccgc ccggattgga aatatcaatt
      361 ctgccagttt gcagagtgtc cctatcagcc gggcaagctg accgactatg cagcggtcaa
      421 attcacagtg aaaggagcga acaacgctat ggcagttttc cgtgtggaaa agaacaaagg
      481 ctatacggtg atgtcaaatc accacctgcg gaataagaac ctgaccttaa aggccaaggg
      541 cctgctgtcg cagatgttgt ccttgccgga ggattgggac tataccctaa aaggactgtc
      601 ctatatcaac cgggaaagca tcgacgcgat caggacagcg gtatgggagc ttgaaaaagc
      661 tggatatatc cggcgtgagc agggccgtga cgcaaaaggc aaaatggccg atatggtcta
      721 taccatctac gaacagccgg tattggaaaa cccggtattg gaaaatccaa catcggataa
      781 cccggtgttg gaaaatccga catcggataa tccggcgtcg gaaaatccaa cgcaattaaa
      841 taaagaagta caaagaactg acttatcaaa aaaagaaaaa ataattacag atggacaaag
      901 tacccattcc attcctatcc catccccgac ccctgcccct atggagggcg aggcagcggc
      961 tacgccgccg gaacggaaaa ggacaggaaa ggatgaggct gtacggattt atcgggaaat
     1021 cattttggag aacatcgagt atgcctatct cattcaggac aggagcattg accgtgaaca
     1081 gcttgacgaa atcgtagacc tgatgctgga aaccgtctgc accgcccgaa agacaatccg
     1141 tgtcgccggg gacgactacc ctgccgagct ggtgaaatcc aagtacatga agctgaccgg
     1201 cgaacatatc cgctttgtcc ttgactgtct gcgggagaac acaacgaaag tccgcaatat
     1261 caaacaatac ctgcgggcta tgctgttcaa cgcccccagc accatttcca actactacac
     1321 ggcccttgtc gcgcacgaca tggcacagcc cgattgggga aagcctaaat ccggcctgcc
     1381 ggactattcc tgcggcgagg gcgaaagcct gtaacgagaa aaggaggacg accctatgaa
     1441 gcagggcgca ttgatttttg acgagcgaac agaccgttac gacatccgct ttgatctggc
     1501 cgactattac ggcggcctgc attgcgggga gtgtttcgac gtgatgatcg gcggcaaatg
     1561 gaggcccacc cgcattgaaa tggccgaaaa ctggtatctt gtcggcatcc gggccgacga
     1621 cctttccggc ctgcgggtgc ggatttgacc tatatctacc tgcggcgtct ggtggttccg
     1681 ccttagacgc cgcttttgac tttctgacaa ggagggatgc gattgcagga agaagttact
     1741 caaaagacca ttgccctgtc catgaaaacg ggcaagctca cggctcaagt gctgcaagcg
     1801 gcgctgaaaa agtacctgca acaccgggcc aagggcaaaa acacgctgca ccacggccag
     1861 cagagcctaa agcagcttaa aaagcatggg gctgatctct ccaacattga gatcaccgag
     1921 gccaacattg gagcgttcaa gccatgcgcc aagaaatacg gcgtggattt taccttacga
     1981 aaagataaga ccacccagcc gccccactac gttgtgattt tcaaggccaa ggatgcagat
     2041 aaccttgatc tggcgtttcg ggagtttacc gccaagacgc tttccaagga gcagcggccc
     2101 tctatccgca aggttttgtc cgctgtgaag caaaaggcgg cggcccagac caagcagcgg
     2161 gccaaggaga aaatcaagga aagggggctg gaactgtgaa gcctgacgta aagaaattgc
     2221 tgatcctgaa cctcccgtat ctgatttttg tgtacctgtt tgcgaaatgc ggggaggcgt
     2281 accggctggc cgctggtgct gacctgtcgg ggaagctcct gcattttgcg gatggttttg
     2341 cggcggcttt tgccaacccg ctgccaagcc tgcacctgtt tgacctgtgt atcggtatcg
     2401 ccggtgccgt gatcgtgcgg ctggtggtgt actgcaagag caagaacgtg aagaaatacc
     2461 gcaaaggcat ggagtatggc agcgcccgct gggggacggc caaagacata gcgccttaca
     2521 tcgaccccaa gtttgaaaat aatatcctgc tcacccaaac ggaacggctt accatgaccg
     2581 gcaggcccaa agaccccaag acagcgcgga ataaaaacgt gctggtgatc ggtggcagcg
     2641 gttcagggaa aacgagattt ttcgtgaaac ccaacctgat gcaatgcttt ccgaccacgg
     2701 actatccgac ttcgtttgtg gtgacagatc cgaaaggcac tttggttctg gaaaccggca
     2761 ggatgttcca gcaggcgggc taccgcatta aaatcctgaa taccattaac ttttccaagt
     2821 ccatgaagta caaccccttt gtctacatcc attcggaaaa ggacgtgcta aagctggtaa
     2881 ataccctgat ttccaacacc aagggcgagg gggaaaagtc agcggaggat ttttgggtga
     2941 agtcggaacg gttgttttat tcggccctga tcggctatat ctggtacgag gccccagcgg
     3001 aggaaatgaa cttctcaacg ctgctggaaa tgataaacgc cagtgaagcc cgcgaggacg
     3061 acccggagtt tcaaagcccc gtcgatctca tgtttgagcg tttggagcag aaagaccccg
     3121 accattttgc ggtgcgccag tacaagaaat tcctgctgtc cgcaggcaag acccgttctt
     3181 ccatcctgat ttcatgcggt gccagacttg cgccctttga catccgggag gtacgcgagc
     3241 tgatggagga cgacgaaatg gagctggaca ccatcgggga cgaaaagacg gtgctgtatc
     3301 tcattatgag cgatacggac acgaccttta atttcattct ggctatgctc caaagccagc
     3361 taatcaacct gctttgtgac cgggctgatg atgtgtacgg cgggcggctg cctgtccatg
     3421 tacgcctgat tttggatgaa tttgccaaca ttggccaaat acccaatttc gataaactca
     3481 ttgccaccat tcgaagccgt gaaatatcgg cctctattat tttgcagagc cagtcgcagc
     3541 taaaagcgat ttacaaggac gccgccgaga tcatttcgga taactgcgat agtgtgcttt
     3601 tccttagtgg gcgcggcaaa aatgccaagg agatttccga tgcgctgggg aaagaaacga
     3661 ttgacagttt caacaccagc gagaaccggg gaagccaaac gtcccacgga ttgaactatc
     3721 aaaaattagg aaaggagttg atgtcccaag acgaaattgc cgtgatggac ggcggcaagt
     3781 gtatcttgca gctacggggt gtgcgcccgt ttttatcgga gaaattcgat attaccaagc
     3841 atccccggta caaatacctt gccgacgccg acaagaagaa tacctttgac gtggacagat
     3901 ttttgactat cacgcgccgg aaacggcagc aagtggtaac gcaggatgaa gtattcgacc
     3961 tgtatgaaat cgacctgtcg gacgaggacg ccgccgctga ataaacagcg gcttttttca
     4021 ttccaacaac aaaatcaaaa aatcattatg agccggaaac cggcagaaag aggtaaattt
     4081 atggcatttt ttaattccgc agttactact ttgcagacta tcgtgatcgg actgggcggc
     4141 gctctgtgcg tatggggcgg catcaatctg ttggagggat acgggcagga caatccgggg
     4201 gcaaaatcgc agggtgtcaa gcagcttgtc gctggcggcg gcgtggccct gatcggtgtc
     4261 actctggttc ccctgctgtc cgggctgctg ggctgatcct ggcctttgtc cctgtccaaa
     4321 cccttaacta atcgcctccg cgtcctcccg gccagaccgg gagggcgcgg gaaaggaggt
     4381 gctacccgtg attagtgaaa tcattgaaga atggataaaa ggcatcctca tggacggtat
     4441 cacgggcaac ctgtccgggc tgttcgatac ggtaaatgcc aaagtcggag aaatcgccgc
     4501 cgacgtggga cgaaccccgc aggcatggaa cagcggtatt ttcaatatgc tgcacagcct
     4561 gtcggaaacc gtgatcgtcc ccatagccgg ggccatactc gcccttgtca tgtgctatga
     4621 gcttatccag atgataacgg aaaagaacaa tatgcacgac tttgacagct ccatgttctt
     4681 ccgctggata ttcaaatccg cgtttgcaat cctgatcgta acgaacacat ggaacatcgt
     4741 catgggcgta ttcgacgcca cccaaagcgt cgtcaatcaa agcgccggtg tgattatcgg
     4801 gcagacatcc attgatttta acaccctgat accagacctt gaaagccaat tggaggctat
     4861 gagcgtgggc gctcttttgg ggctgtggtt ccaaaccctt gttgtgggcc tcaccatgaa
     4921 tatcctttcc atctgcattt tccttgtgac ctatggccgc atggtggaaa tctatgttgt
     4981 cacgtcgctg gggcctatcc cccttgccac aatgggcaac gcggaatggc gcggcatggg
     5041 ccaagggtat ctgaaatccc tgctggcgct gggcttccaa gcctttttaa tcatggttgt
     5101 ggtgggtatc tacgcggtgc tgatacagaa tatcggttcc gcttccgaca tatccggcgc
     5161 aatctggggc tgtatgggct atacggtgct gctgtgcttc tgcctgttca agaccggcag
     5221 tatatcaaag gccgtattcg gggcgcattg atcccaaaat ccaagcatac acaggaggaa
     5281 cttgtttatt tcaacgtaca acatcattta cgctgacccg ccgtggcggt atgactgcaa
     5341 aaacgtacag ggcgcagcgg aaaatcacta ttccaccatg agcattgacg agctgtgcgc
     5401 cctgccggtg gaacggctgg cgtccaaaga ctgcctgctg tttttgtggg ccacattccc
     5461 catgctgccg gaagctctgc ggttaatcaa agcgtggggc ttttcattca agaccgtggc
     5521 ttttgtatgg ctgaaacaga accgtaaaag ccttacatgg ttttacgggc tgggccgctg
     5581 gacaagggga aacgcggaaa tctgcctgct ggcaacacac ggcaagccga agcgtcgttc
     5641 tgcctctgtc catcagttta ttatcagccc gattgaacaa cacagcaaaa agccggatgt
     5701 gacccgtgaa aaaatcgtag agctggcggg cgatttgccc cgcgtggagc tgtttgccag
     5761 acagaaaacc cccggctggg acgtgtgggg gaacgaggtg gaatgtgacc ttatcctgac
     5821 gtgaaaggag gtgccaaatg gcttatgtaa ccgtccccaa ggacttaacc catgtgaaaa
     5881 gcaaggttgt gtttgggctt accaaacggc aactgatctg tttttccggg gcgctggtga
     5941 tcggcgtccc cttatatttt ctcacccgtg actatcttac caacagcgcg gcggccctgc
     6001 tcatggtatt tgccatgctg ccgggctttt tgcttgccat gtacgagcgt cacggccagc
     6061 cccttgaagt ggtgattatg cagatcatcc agtgctgctt catccagcca aaggagcgtc
     6121 cctatcagac cgacaacacc tacaccgccc ttgtgcggca ataccagatg gaacaggagg
     6181 taaaggccat tgtccaaaaa ggaaatccca agagaaaccg aaaaaacaaa gctgacccgc
     6241 gcccagcgaa aagaaattga cgcggtaatc cgtaaatata agggcgacgg caggccccac
     6301 acggcgcagc agagcatccc ctacgaggtc atgtacccgg acggcgtgtg ccgggtatcg
     6361 cccggcgtct tttccaagtg cattgagttt gcggacatca gctatcagct tgcccagccg
     6421 gacacgcaga cggctatctt tgaaaaactg tgcgacctgt ataactatgt ggacgcctcc
     6481 attcatatcc agttttcttt cctcaatcgg aaagttgacc cggtgcagta cgccaagagc
     6541 tttgagatcg cgccgcaggg ggacgacttc gacgacatcc gggccgagta caccggcatt
     6601 ctgcaaaagc agcttgcaaa cggcaacaac ggcatggtaa agaccaaata cctgacattc
     6661 accatcgagg ccgaaagcgt gaaagcggcg cgagccagat tgaagcgcat cggctttgac
     6721 ctgttaggct atttcaagag catgggggcc gtagcccatg tgatggacgg ctgggaacgg
     6781 ctgaacctgc tgcatggcgt gtaccacccg gacggcgaga tattcaattt tgactggaag
     6841 tggctggccc cgtccggcct ctccaccaag gattttattg ccccgtcctc cctgtgcttc
     6901 ggcaatgcaa agacattcgg catgggcggc aaatacggcg cggtaagttt cctgcaaatc
     6961 ctgtccccgg agctgtcgga cgatatgctg gccgacttcc tcaatacgga aagcggtgtt
     7021 ttagtcaatc tccatgtgca ggccattgaa cagaccaagg cgatcaagac catcaagcgc
     7081 aaaattaccg accttgacgc tatgaagatt gcggagcaga aaaaggccgt ccgcagcggt
     7141 tacgatatgg acattcttcc cagcgacctt gccacctacg gcgaggacgc caagaagctg
     7201 ctgacaaagt tgcagacccg caatgaacgg ctgttccagt tgacgttcct tgtcctcaac
     7261 gtggccgaca ccaagcaaaa gctgaataac gacgtgttcc aagccgcagg cgtggcccaa
     7321 aagcataact gtccccttgt ccggctggac taccagcagg aacagggcct tgcaagcagc
     7381 ctgccgctgg gcgtcaatca gatcaagata cagcgcagcc ttacgacttc cagcgtggcc
     7441 gtgttcgtcc cctttgtgac gcaggagctt tttcagggcg gcgcggccat gtattacggc
     7501 atcaacgcca agtcccgcaa catgattatg ctggacagga agcaggcccg ttgccccaat
     7561 gccttgaagc tcggcacccc cggcagcggc aaatccatga gctgtaaatc cgagatcgtc
     7621 agtgtgttct taacgacgcc tgacgatatt tttatttcag acccggaggc cgaatattac
     7681 ccccttgtca aacggctcca cggacaggta atccggctgt cgcctaccag caaggacttt
     7741 gtgaacccgc tggacatcaa cctgaattat tcggaggacg ataacccgct ggccctgaaa
     7801 tccgactttg tgctgtcgtt ctgtgagctt gtcatgggcg gcaagaacgg gctggaagcc
     7861 attgaaaaga ccgtgataga ccgggctgtg cgcgtgatct accggcccta tctggcagac
     7921 ccccgcccgg agaatatgcc gatcctgtcc gacctccaca aagcgttgtt agatcagcat
     7981 gtcccggagg ctgaccgggt ggcgcaggcc cttgacctgt atgtgtccgg ctccctgaac
     8041 gtatttaacc accgtaccaa cgtggacatc ggcaaccggc ttgtgtcctt tgacattaag
     8101 gagctgggca agcagctcaa aaagctgggg atgctcattg tccaagacca gatatggggg
     8161 cgcgtcacgg ccaaccgaag ccaaggaaag gccacatggt attttgccga cgagttccat
     8221 ttgcttttga aagaggaaca gacggcggcc tattctgcgg agatttggaa gcggtttagg
     8281 aaatggggcg gcattccaac aggtgccacc cagaacgtca aagacctgct ttcctccccg
     8341 gaaattgaaa atatccttga aaacagcgac ttcattacgc tgctcaatca ggcgtccggg
     8401 gatagaaaaa tccttgcgga acgcttgaat ttgtccaccg aacagcagaa gtacatcgac
     8461 aattccgaac ccggcgaggg ccttttgatc tttgaaaacg tggtgctgcc ctttaccaac
     8521 cccatcccgc acaacaccca gctttacaag attatgacga cccgcttaaa cgaggtggcg
     8581 ggagtatgac ccggcgacgc cacaaaggag gtgaaccacc atgaaagagc tgaaagccaa
     8641 ggcgaaagtg acgcaggcca tgacccgcga cgggctgacg ctggaaaatc aggccaccgg
     8701 cgaggtggag aacatttcca gccgggaggc cgaacaggat ttgtccgcgc cggggccggg
     8761 cggcaccgcc gataagctgc tggaacgggc cgacgccatc catgacggcc ataaggccaa
     8821 gaaagccgcg aagcaggccg gggctgtggc cgccgagggg gacgctgccc tgcaccggcc
     8881 atccgcccgg ctccaactgt cggaggaaga acgggccgac ccggatttac agccctatat
     8941 ccgaaaatcg gacaaacggg ccgataagct ggataaggcc cgcgccgcca tccccaagaa
     9001 gcgcgtcccg gtcaaggaaa ccgtctttga cgaggcaagc ggcaaagcaa agaccgtcct
     9061 gcactttgac aaacaggaca aggggcctcc ctccctgaaa cccaatccgg caggccgtcc
     9121 tgtttccgag gtttttctat tcggccacgg gaaaatccat gaagtcgagc atgaaaacgt
     9181 gggtgtggag ggcggccata agggggagga acttgccgag cggcaggccg gaaaagccat
     9241 ccgcagcacc atccggcacc acaggctgaa accctactgg gacgcggaaa aggccgagcg
     9301 caggctattg tctgccaacg cggagtattt ctaccagaaa tctttgcggg ataacccgga
     9361 gatcgcacag gcggccagca accccatttc ccggttctgg cagaaacagc agatcaagcg
     9421 ccagtacgcc aaggccgccc ggacagcggg ccagaccgca ggcgcggcgg cacagggcgc
     9481 ggccaagaca gccggggcct ccacgaaagc ggccaaggcc gcagccaaag aaagtgaacg
     9541 ggcggcctcc tttgtggccc gccactggaa aggggcgctg gccgtccttg tgttggggct
     9601 tttgatgctg cttatcatcg gcggcttaca gtcctgcacc gccatgttcg gcagcgccgg
     9661 aagcggcatc acggcgtcct cttacttttc cgaggatagc gatatgttgg ccgccgagga
     9721 cgcctacgcg gagctggaag ccgagctgca agactatctg gacaactacg aggccaccca
     9781 tgactatgac gaataccatt ttgaccttga cgagatcagc catgacccct atgtgctgat
     9841 ttccattctg tcggcgctcc atgacgggat atttaccatc ggggaggtac agggtgattt
     9901 gtccatgctc tttgacaagc aatatatcct gacagaaaac gtcgtggtag aaacccgtta
     9961 ccgcaccgaa accgatacat ggacggacgc ggagggcaac acccatacag acacctacca
    10021 agtgccgtat gactattaca tctgctacgt cacgctggaa aacttcgacc tgtcccatgt
    10081 gccagtctat atcatggacg aggaaacgct aagtttctat tccctgtata tgtccacgct
    10141 aggaaaccgg cctgatctgt tttccggcag ccagtacccc aacgcctcca ccctgacgcc
    10201 gccgacctac tacgacgtac ccccggaggc tttggaggat gccacctttg ccgccatgct
    10261 caaagaggcg gaaaaatata tcggttatcc ctatgtgtgg ggcggcagct ctcccagcac
    10321 cagctttgat tgcagcggct tcatttcgtg ggtaatcaat catagtggat ggaacgtggg
    10381 acggctgggg gcgcagggcc tatataacat ctgcacccct gtttccagtt cgcaggccaa
    10441 gcccggcgac cttgtatttt tcaagggaac ctacgacacc cccggcgtgt cccacgtcgg
    10501 cctgtatgtg ggcaacaatg tgatgctgca ttgcgggaac ccgatttcct acacgaacct
    10561 tacttcaaac tactggcaat cccattttta cagttacggg cgtttacctt aacgccagaa
    10621 aggagctttc aattatggca accaaacttg accgcatcga aaaggacatc cagaaaacca
    10681 agtctaagat cgcggagttt cagaaacagc ttcgggagct ggaaacgcag aaaaccgaac
    10741 aggagaattt gcagatcatc cagcttgtgc gcggcatgaa catgaagccg gaggaatttg
    10801 ccgcgttctt acgcagcggc gcaatgcagg ccgcccctgc cgcaacccct taccatgaac
    10861 aggaggacaa tgcccatgaa gaataagacc agacggattt cccgtattct ccccgcactt
    10921 ctggccgtga tgctctgcgt cacggccttt cccgttaccg cccttgcagg cggcaatgac
    10981 ccggcaccgg ccccgctgcc ggaggctaca accgaacccg ctacgggcgg tttggagcct
    11041 gaaacagacg aaaccggcac cgccacggac gggacagaaa ccgaggccga aaaagatacc
    11101 ggccctctga ccggcaagga catcaccgac ctgttttccg cgctgttcgg ttccaaggtg
    11161 agcattgccg ccacggacga cggcatccag atcaccaccg gcaccgacaa aaaagaacct
    11221 gaacagaccg gcaccgtcac caccaacggc ggcaggctga atgtccgcac cggcgcgggg
    11281 cttgacaaga ccgcgttcac ccagcttccc aatgggacga cggttgaggt agtcggcaca
    11341 gacggcgact ggattaaaat tctcttgccg gagcgcatcg gctatgtcca ttccgactat
    11401 atgaccgtca gcgaaaagga agtagcggct accggcgagg gcggtttttc cctctccatt
    11461 gacccggagg aaattgcttc tctgctggaa ctgttcaatg gcggcggggc cagcgccgcc
    11521 ctgtccccgg acgggaacct gtcactgatt gacgacatcg gcagcgccgc gaaaaatggc
    11581 aagcagttta tcaccgtgga aaccaaaaac ggcaatgtgt tttatctgat tattgaccgg
    11641 gacgacgagg gaaaagaaac tgtccatttc ttaaatcagg tggatgaagc cgatctgctg
    11701 acgctgatgg aggacgggga aaccgtcacc cccgctgctg tctgctcctg taccacgaaa
    11761 tgcaaggccg gggctgtgaa cacgaattgc ccggtatgta aaaccaacct gaccgagtgc
    11821 agcggcccgg agccgcagga accccagccg gaggaacccg aagccccgga ggaagaaccc
    11881 aagggcggcg cgggcggcct gatcgtgttc ctgctggtgg cgctggcggg cggcggtgcg
    11941 gccctttact atttcaagtt caaaaagccc aaggctgaaa ccaccggcag cgacgatttg
    12001 ggcgagtatg attttgggga ggacgaggat ttggaggacg aggaacccga aaccgagttt
    12061 gacccggaag ctgattttgt gagtgaacag gaggacgagg aatgacatat aaacttgtaa
    12121 tcgcggaaaa acccagcgtg gcccgcagca ttgcgggcgt gatcggggcc acggagaaac
    12181 acgacggcta tttggagggg aacggctatc tggtgagctg gtgcatcgga catctggtgt
    12241 cctttgccga cgccggacgc tatgacgagc gtttcaaaaa atggcgctat gaggatttgc
    12301 ccattctccc ggaaacatgg cagtacatcg tccccgacga caagaaacag cagtttgacg
    12361 ttctctgttc gctgatgcag cgccccgacg tgaccggcct tgtgtgcgcc accgacgccg
    12421 ggcgcgaggg ggaattgatt ttccgtttcg tctaccagat ggcgggatgc caaaagccct
    12481 ttgagcgcct ttggatttcc tctatggagg acagtgctat taaggacggc tttgcccacc
    12541 tgaaaccggg cggcgactat gatccgctgt atcaatcggc cctgtgccgg gccaaggccg
    12601 actggctgat cggcattaac gccacccggc ttttttccgt cctgtaccac aagaacctga
    12661 ctgtgggccg cgtccagacg ccgaccttaa aaatgctggt ggacagggac gccaagatta
    12721 ccgatttcaa gaaagagaaa tatcacatcg tccatatcac ggcgggcggc gcggacgcgg
    12781 taagcagccg cttttccgac gctgccgagg caaacaccgt caaggcggct tgtgcaaaag
    12841 cgcaggccgt ttgcgtttcc gtgacgcggg agaaaaagac ggaacagccg cccaagctct
    12901 atgaccttac caccttgcag cgggaggcta accggctttt cggctatacc gccaagcaga
    12961 cccttgacta tgcccaggca ctgtatctaa agaagctgct gacctatccg agaacggata
    13021 gtcaatatct taccgacgat atgctgcccg ccgtggaaag ccttgtgtcc ggcctgtggg
    13081 agctggtgcc gtttgccaag ggcctgaacc tttccccgca gtttgaccgc atcctgaaca
    13141 gcaaaaaggt gtccgaccac cacgccatta tccccaccgc tgaatttgtg aagcagggct
    13201 ttggcactct ggccgagagt gaaaaaaagc tgctgtccct gatctgctgc aagctcttat
    13261 gtgccgtggc cgggccgcat atctatgagg ccgtcaccgc cacatttacc tgtgccgggc
    13321 aggagtttac cgccaagggc aagaccgtcc tttcccccgg ctggaaagag attgaacgcc
    13381 ggttccgttc ttccttaaag accgacgccg acgaggacgc cgaggcggtg cgggagctgc
    13441 cggagctttg cgagggccag acctttgcag acgtggccgc ctctgtcacc gagcatttca
    13501 ccacgccgcc caagccctac accgaagata cgcttttgtc cgctatggaa cgggccgggg
    13561 ccgaggatat gccggaggat gccgagcgca agggattagg cacaccggcc acccgcgccg
    13621 ccattctgga aaagctggtg cagatgggct ttgtccagag aaaaggcaag cagctcattc
    13681 ccaccaagga cggcatcaat ctggctgtgg tgctgccgga ggcgcttact tctccccagc
    13741 ttactgccga atgggaaaac cggctgaccg agatcgccaa ggggcaggcc gatcctgacg
    13801 aatttatgga cggcatcgag acgcaggcgc gggagctggt gcagacctat tcctgtatca
    13861 gtgaggaaaa gcagaaattg tttcaggccg agcgtgttcc tatcgggacg tgtccccgct
    13921 gtggtgaggc tgtctatgag ggcaagaaaa actactactg cggaaaccgg gcctgccagt
    13981 tcgtcatgtg gaagaatgac cgattttttg aggaacgcaa gaaagcgttc accccgaaga
    14041 tcgccgctgc gctgctcaaa accggcaaag tcaaggtaaa gggcctctat tccgtaaaga
    14101 ccggcaaaac ctacgacggg acggtgcttt tggctgatac cgggggcaag tacgtcaact
    14161 accgcattga acacaggagc taaaggacag taacacagca agcataggga gtgtggctac
    14221 actcccggta aaatcccccg gccgcgtccc tgccgggccg gggatttttg cttgttgggg
    14281 aagtccccaa ccccctattt tttgaaacag aacagagaaa ggagtgaccg cgtgaacgaa
    14341 gtattttcta tccaagtcca taaccggcag tttttggaac agggcaaaca aggcgtctgg
    14401 ctggatttgc ccaccacaac cgaaacgctg caagccgccc tgcgggatgt tggcattact
    14461 gccgacaatc cgcaggattt ttttatcaac gggtattcct gcccggagga cagacacctt
    14521 gccattccct atgacatggt gctggccgcc gacgtggacg agctgaattt ccttgccgcc
    14581 cggctgggcc agcttgacgc cgccgagatc gcagagctga acgccgccct gcaaaatccc
    14641 cgtggcggct ttgagaatat cggccagatc atcgactaca ccgaaaatgt ggattactat
    14701 gtgcatcttc ccgacgttag gggggatgga cagttgggcg actactacct gaaccgttcc
    14761 ggcatggtgg atatgccgga ggaatggaaa gccgggattg atacggcgca gtttggacgc
    14821 cacattgccc agcaggaaca aggcgttttt acccagtacg gctatctggt gagaagcggc
    14881 gacgaatggc agcgcgtcca tgagggccag ccggtgccgg aggaataccg ggtgatgcac
    14941 tacccccagc cggaagctga ccgggaggaa ccgcaggccc agcagccgga ggcatccgaa
    15001 agccccgaca aagcgccgcc cccggttatc ccgatcatgc tggacggcat gaacagcaaa
    15061 gaacgcatga aagtgattac tgaccgtctg gaaaccggcg tacaggagct ttttgaaagt
    15121 agccgctata tggcttactt atccgctatg tccaagatgc acgattacag tctgaacaat
    15181 acgctgctga tcgccatgca gggcggttcg attgtggcgg gctataacag gtggcaggat
    15241 gaatttgacc gccatgtgaa gcggggcgag aaaggcatta agatactggc cccggccccg
    15301 ttcaaggtta agcagcagat ggagaaaatc gacccggcca cccagcagcc cgtgatcggc
    15361 gcggacggca agcccgtgac cgaagaacag gaagtgacgg ttccggcctt taaggtggtg
    15421 acagtctttg acatcagcca gaccgagggc aaggattttc ccatgcccta tatcagtgaa
    15481 cttaccggcg acgtgcagca gtatcaggat ttctttgccg cgctggaacg gacatcaccc
    15541 tatcccataa gcattgagcc gatttcagac ggggccaagg gccgctgctt ctacgcaaaa
    15601 cagcgcattg ccatcaacga gggcatgagc gaattgcaga ccctaaagac cgccatccat
    15661 gagattgccc atgccaagct ccacgccatt gaccgggagg cccccgccga ggaacaggcg
    15721 gcccggccag accgcagcac ccgcgaggta caggccgaaa gcgtggccta taccgtctgc
    15781 cagtattacg ggctggacac gtcggactat tcttttggct atgtggccga ttggagcagc
    15841 ggcagggagt tatccgagct gaaagcctcc cttgaaacca tccgggccac ggccagcgaa
    15901 ttgataaccg agatcgacgg ccattttgcc cagcttcagc aggagcgcga ggcccggcag
    15961 gaacaggagg ccgtcacggt tgacgttacc ccggaggcag cggccccggc gcaggccgag
    16021 gaagaacagg ccccggccct tgatccggca gccgagccgc ttgtgactgt gctatggagc
    16081 gaaagccccc agctaaagga cggccagcaa atgccgttgc atgaggccga cgcgctgttt
    16141 gcccgtctgg atgcagcata tccggcagag gcaggctatg acaaaaccgc gttccgcatt
    16201 gactgtatct ttcagggcga gccggacagc tacgaggggc ggcaggattt tggagatagg
    16261 gacggttccc tgatcgaaca tatccagagc taccaccaat tttacgcgca ggacgaaaac
    16321 tggaaaaagc atgtaatcgc tacaaccggc atagagggct gggagcagga caaggcccat
    16381 cgggatatgc tgttaggcga gtttgtcccc tatatgaagc tgcattgcag ccttgcccaa
    16441 atggaacaga cggcgcaggg ccgcttacag tccggggaaa ccctgacgcc ggaggaaacg
    16501 gcctatttgg gggcgcttct ctcttatgtg caggagtgcc gccccatgct caatcagggc
    16561 cagtatcagt tgccggagcc gccgaaactg gctgacttcg accagagctt gcaggactac
    16621 aaagcacagg tacaggccga gatcgcgcag gaggccgccg cagccggtat gaccgtggag
    16681 gaatacgcgg cggctggcta tgaagcaccc caacaggaca ccttttccat ctaccagctt
    16741 cgaggcgggg aggaaacccg cgactatcgg tttgagccat acgagcgttt acaggccgca
    16801 gggctggccg tggacagtgc caactatgac cttgtttata cggcaccgct ggataaaagc
    16861 acgtctttgg aggatattta ccgcacattc aacatcgacc acccggcaga ttttagggga
    16921 cacagccttt ccgtttccga cgtggtggtg ctgcaccaga atggacagga caccgcccac
    16981 tatgtagaca gcttcggcta tatggaagtg ccggagtttt tgcgggaaaa tcccctgcgt
    17041 accgccgaac tgtctacgga gcagaacgaa aacatgattg acggcattat caacaatacg
    17101 ccgcccgaac cgcagccgga ggaacccgaa cagctatctt ttgaggatgt ggcagttgct
    17161 tccggccaga cacaggagga acccccggag gctgcgcccc cgcaaacggc tgtccgctat
    17221 tatcccatca atgagggagc cgcccgccgc gccaaggaag ccatgagctt ttctgactac
    17281 cggcccggca gcgccacggc ggaataccgg cactatgtcg atcaggccgt ggaaattgcg
    17341 gagcgccaga aaaagcgtgt ggagccagag tatcacgaaa agatagacag cctccttgac
    17401 acctacgccc gcaagctggc cgagaacatg aataagggct atgagattac cgcccgcgtc
    17461 ccctctgtcc tgattgccgg gccgtccaat ttcccggtac gggcgaaaga aaagcagaac
    17521 gccgccagtg accgcaacat ggaggaattt cagtacattc agggcttgct tgacaaaatc
    17581 cgcagcaccg gcatgggtgg tatcagcgcc gacgatccgc aggccgtttc caagctggaa
    17641 aagaaactgg aaaagctgga agcgtcgcag gagttgatga aggccgtcaa tgcctactac
    17701 cgcaagcatg ggacgctgga cggatgcccc catctgaccg aaagaggcat tgaaaacctg
    17761 aaagcggata tggcgtcggg ctggcactat gagaaaaaac cgttccagtc ctggcagctc
    17821 cccaacaata acgccgagat acgccggtta aagggccgca ttgaggaatt gacccgccaa
    17881 aaggaggcgg cctatgtggg atgggagttt gacggcggca ctgtggagat caaccgggtg
    17941 gccaaccgcc tgcaaatctt ctttgagggg aagccggatg ctgctgtacg cgacgagctg
    18001 aaaagcaacg gtttccgctg gtcgcccaag gccggggcat ggcagcggca gttaaacgac
    18061 accacaatcc gcgtcgctga ccgtatcaag tgcatccagc ccctttccgg cgaaaagccc
    18121 agcgccttac agcttgcggc ccgcagggag aaagaaaagc cctctatccg ggcgcagctc
    18181 aacgcggcca agaccgaaca ggccaagcag ccaccggcgc gggaaaaatc aaagggattg
    18241 gaggtagagt aaatgcagaa gtttgaaaat gtggacgttc tggccgcgct ggaacagatc
    18301 atgcggcaga acaccgcgtt ttatcaaagt gactttggca ttgacaaaga cattatcagg
    18361 cgggatgccg ccagcgatca ggccgcagac aagaccctgc tctggatgtc ccggccctcc
    18421 gggacgtact gtttccggga acgtgatgtt ttcctgaaag acacccgaca gtacaacaca
    18481 tggaaatttt acggggagca gacccgtgac aagatactgg cctacgcggt ggagctgacc
    18541 ggcacacagg gcggcactat ccggggcaat ctctatgagc tggactatcc gcagcatttc
    18601 cggcacgtca ttgaaaccgc ccagccggtg agtgtgaacc ggctctttta tgagcatggc
    18661 acccgtgaca tcccggagag ccagcgtttt gacggaagcc ctgaccgggt attgggaaac
    18721 tttttgtact atgaggccca gccccatgac ccggctgtgc tgcaggaggc gctacggacg
    18781 gagcagcaca gccgggggcg ggccgtcccc ggcgatttca aagtccacgt caccgctttg
    18841 catgacagcc ggattgaaac cgaggcccgg cgtattgtgg acagcctgaa agatctgtcc
    18901 gcgcctaaca gccccaataa aacgcatttc atggtggaga tctccccgca ctttgcggcg
    18961 attgcttcca ccaaggataa tgaccggctc tttgccatgc tgccctataa atccctgtgt
    19021 tttaccggga tgaaaggcag gcatggcgtg tatgccgtga ttaaccggga cgagcgccgg
    19081 gatgtgagta ttcgcaggcc ccggccctct atccgcaagc agctatccga cagcaaacag
    19141 gccgccagcc caaaaaaggc ggcggccaga accaaaaaga atgaattgga ggtataagga
    19201 atgatgcact ttaccgtgga ggaagaaaac ctgatctgta tgtaccacaa tgcagaccgg
    19261 cgcagaacga taaacaggct caccgccgtc atgccggtta tggatgggga tatgcgggcg
    19321 caggcccagc agaccctttc caagctggaa cgcatgacgg atgccgacta tgacggccag
    19381 aagttccact tcacagacga ataacgaaca gcagacgccg ggcgcggcgg ccatgtggtc
    19441 gcttgcgtcc ggcgtctttt ctttttgcct attcctacgc atttagaacc cggtattgtc
    19501 ggggatagga tgaagtattc gcttgcccct gcaaacatgc gggagaagcc ttgcagcggg
    19561 cggttttcaa tcccttaaat gcgtaggttg cagctataat tagacccctg aaacagatat
    19621 agccgcgcag cgggtgtatc tgtttcaggg gtgtgcaggt gatagccgca aagcggcgta
    19681 agagggtgca gccctcttgc cgtctatctc tgttccagcg ggaagctcgg caatgcttcc
    19741 agcagtttca gggaaatgcg ctgccgtata tcttcgtcaa tgactttctt ctttttgccg
    19801 tttgcctgtc tgatctccac cgtggaaagc tcgttgatgt agtcagcata atgggccaac
    19861 actttttccg tggcccacct ctcaccggca acggcggctt tgatcgtttc atagtccagc
    19921 agcttttctt gtccgtccat cctgataccc ccgtcagact gattttttct atgataccat
    19981 attcggaagc atggaaccag agaaaacggc gcttttctgc tggtaaattc tacgcattta
    20041 gaacagcact tcggcgggga taggataaaa cacccacata ccccgctgac catgccggaa
    20101 aagccttgaa atataagggc ttttacagct ctaagtgcgt aggtttccag tatcttttcc
    20161 gctggcgctc ggcctctttg cggcggcgca tccgtttgga gcaggccgcg cagtatttgg
    20221 cccggttaga cttcggcgtg aacgtggccc cgcactcaca gcagcgtttc acttcgtccc
    20281 ggtctttggt gatctcggca tacagggccg catccagcgg gaggacagcg gccttaaacc
    20341 agcggcacag aagcgaatag gaaatgctct gtacgcaaat acattcttcg ccgtcgtcca
    20401 gcaacaggca gttgccgtca caatagttgc agcactcatg cgtcagcttc cgggccttgc
    20461 gatattgagg gtaattcatt cgtggaatac tcatagctct tgcgtccatt cctttcccgg
    20521 cgtcatacgc aaaatgccgt ccacattctg cttgatgatg ccgtattcct gcatctgctt
    20581 tttcgcttcg ccgtacttct catacagccg ttccttttcc tcggccaatc gcttgtactc
    20641 ggctttcagg gcgtacagat cggggagctt tttcacaccc tgtcccttta gggcctttgc
    20701 cgcagcttca tacaaaatca acgtgctttc atgctcacgc cggaattttg ccttatccgc
    20761 tgcattgcgg tattccatag ccacgggcca gagctgcttg taggtagaaa tattctttat
    20821 cagcaccgcc atatccgcaa gccggtgttc catagctttg agggtagcgg cggcctcgtc
    20881 gctggcggcg gtgatctcgg ccactttgct ttccaaatcc gggtaggtgt ctatcccatg
    20941 ttcagtcagg aaattcaaag tctttgcggc ctgcttcaaa ttgtggagct tcgcccacct
    21001 ctcatagcct gccgactgct gggccttaat gttgttttcc agatcaatcc gcaaagaaat
    21061 ccccttgcgc tctttgggcg ctctggtgat aacgcggcct ttgatacgct ctttgatggc
    21121 ttcctcggtg taggcttccc ccaatgtctt gcagcgggta aagcgttcct gtccgggagc
    21181 gcggaaagaa ataaactttc ccggcttgat ctcatagccc tgcgcctgca tgagccgcag
    21241 gaaatcatca aaatcttttg cctgcgggat agcggcgtca atggctgctt tgagcttcgc
    21301 tttccagctc gtccctttcc gctgggcgtc ccactcggca tagctcttgc ccttactctg
    21361 gccgggcttc accacggaca atccgttttc cttgcacagc ctgtcgctgg tgcgccggat
    21421 ataggcatag ctctgtttgt tggaaatgta cttgcgctga tttaccatat ccacggcaca
    21481 gaaaataatg tgattgtgga cgtggccctt atcaatatgg gtagtcagca catagggata
    21541 tttcccttgc agcacttcct cggcaagctg cttcccgatc tcatgggcct gttccggcgt
    21601 ggcttcgccg ggggcaaagg attgtatcag gtggtggccc agattattgc ccttttggta
    21661 tgcctgcgcc agcagcattt caaattcaat gtccgctgtt tcataagagc aggcaaagga
    21721 ggccaccagc agcttgccgt cagttttgtc cgggttctcg atgtagtcaa gggctttttt
    21781 cagcgttgac ttgataggat gtatctttgt aactgccata tctgcgccag cgcccccttt
    21841 atatcctcga tgtcctgtgc gtacaccggc cccataccgt tcagcctctt tgcaatctga
    21901 ttgacgttga cgccgatctt ctgtatctcg gcggtctgcg cccggatgtc gctggtgtcc
    21961 atgttgacga tatagccgtc aatgagcatc ttccgggcgt aggctgaaaa gttcttcgta
    22021 tgcagcagcg ccattttctg ttcaatcata gcccgttcct ccggcgtcac agggacgcgc
    22081 agcacgattt ttcgctttct gtttgccatg cttcaccgtc ctttccattc aggggtttgg
    22141 ggatttcccc aacaagcaaa acggacgcgg cccggcaggg acggggcagg ccgtttttcc
    22201 gggagtggct acactcccta tgcttgctac gttactgttt ctccactaac aagacgtttc
    22261 caaagcccca aactacccgc aaaatagccc tctggaaaga aaaaagtgca aaaaaataga
    22321 agctacgctg tgcagctccc gctaaaagta aaacagagga tgccagttac acatcctccg
    22381 tttcattctt cttcggcttt tcaattagta tggcgctgtc aatcgcgccc tcgatgatcg
    22441 gcaggtattc ttcggggcag agtttcagct tgtggctgac cctttgccgc agttcgcttt
    22501 cctcccgcat gatctccggg ttaaagtagc gttccacagg aagcccgcag gctttaataa
    22561 gctgtatgac aaccggcaga ctgggaatgg ccccgtcgtt ttcgatgttg gcaagatagc
    22621 gccagtcaat atttattttc tctgccattg ctttgcgcga aacgcgcttt gcttgccggg
    22681 ccgctttcac atccgcgccg aaagtttcaa agccgggaca atcttcaata ttcgccatat
    22741 cgcacctccc acttacattg tatatttcat actctgcccg tggaatgttg gtatatgcac
    22801 ctttcttaaa gtttatattt cacattttgt ttcttttatt cttcgagcaa aagaattata
    22861 atgtaccctg tggaggtgta ctatggacta catgacgttg aaagaggcag gcgagaaatg
    22921 gggtgtgccc ccacgatgga taaactacta ttgtgctgcg ggacgcatcc ccggcgctgt
    22981 aaagatggcg acgatctggc tcattcccaa aactgcggaa aagccgattg acaggcgtac
    23041 aaagaaagga aaggcggtta ctgaatagta taatgaaacc ggcgcatttt ggatatggtt
    23101 ttgaagagag agaaagcatg gtattttgtc acggaaaaac tataagatga tagatggcag
    23161 gctgctccag accgataaaa agtattctca tctgaaacag aaagaaaaga tttacacatg
    23221 gatgtatgaa gaagcgaaac tttatcatga cagaaatgga aaatgcccga aacaaagacg
    23281 aggaagctgc tgctgtatca gccgtatatg gtagaattga aaaggccggc atctggattc
    23341 tttatggtga agtctccaga cattggataa ctgctggtct aatgcgcctt tgagtatggg
    23401 gtgattgatt ttaataccgg ggaggtacgg ctttttgagg aaaatattct gggatttaca
    23461 tttggcgatt attacatttc ctgtaactgc aaggacacgg atgatgaaga tatgaggaat
    23521 atagacttgc tgattttcta ctgtcttgaa cggtattagg actgaatctg ctgcgcggag
    23581 tgcggcaaga gcgaccggag ggagtacaca caggccctgc ctgtgtgaaa agccataccg
    23641 caaggtgcat tcgcaaaaaa gaggccgcgc attttgctga ggcttggggt gtccccaaca
    23701 agcaaaacga gaacgccgca ggcgggcaag tggtggatca gaatatccgt aaaggatatt
    23761 ctgatccatt gcttgccagt attatcggga tgttttgaca accgctgtaa tcgaaacatc
    23821 ccaaacatta agaaggtgtt aattattatt atcttgattt aattcgttta aaccaataat
    23881 atagatacat acaccatgaa aagaagcgag tatcccaatg acggatgatt aaaatccgaa
    23941 aagaaatcgc tttgaaacta cagggaaact acagactgtt atgttatctt cttaaaatgg
    24001 agggattttt atgtcgatac gaattctact tgtcgaggat gatgatcata tctgtaatac
    24061 agtaagggcg tttctggctg aggcaggata tcaggtggat gcctgcacag atggaaatga
    24121 ggcatacacc aagttttacg aaaacactta tcaactggtt attcttgata ttatgctgcc
    24181 cggtatgaac gggcatgaac ttttgcgtga atttcgtgcg aaaaatgata ctcccattct
    24241 gatgatgaca gccctgtcgg atgacgaaaa ccaaatccgg gcgtttgatg cagaggcaga
    24301 cgactatgta acaaagccat tcaagatgca gattttacta aagcgggtgg aagccctgct
    24361 gcggcgcagc ggtgcgctgg caaaggaaat ccgtgtcggc aggctgacac ttctgccgga
    24421 ggatttcacg gtactttgtg acggtacaga gctgcccctg acacgaaaag aatttgaaat
    24481 ccttttgctg ctggtgcaga acaaaggcag aaccttaacg catgaaatca ttttgtcacg
    24541 tatatgggga tatgactttg agggtgatgg cagcacagtc cacactccca cactcatatc
    24601 aaaaatttgc gggccaagct gccggaaaat atcatcaaaa ccattcgcgg tgtaggatac
    24661 cgattggagg aatcataata atggaaagaa aagggatttt cattaaggtt ttttcctata
    24721 cgatcattgt cctgctactg cttgtcggtg taacagcaac actgtttgca cagcaatttg
    24781 tgtcttattt cagagtgatg gaattacagc aaacagtaaa atcctatcag ccattggtgg
    24841 aactgattca gaatagcgat aggcttgata ttcaagaggt ggcagggctg tttcactaca
    24901 ataaccaatc ctttgagttt tatattgaag ataaagaggg aagcgtactc tatgccacac
    24961 caaatgccaa tacatcaaat agttttagac ccgactttct ttatgtggta catagagatg
    25021 ataatatttc gattgttgct caaagcaagg caggtgtggg attgctttat caagggctga
    25081 caattcgggg aattgttatg attgcgataa tggttgtatt cagcctttta tgcgcgtata
    25141 tctttgcgcg gcaaatgaca acgccgatca aagccttagc ggacagtgcg aataaaatgg
    25201 caaacctgaa agatgtaccg ccgccgctgg agcgaaagga tgagcttggc gcactggctc
    25261 atgatatgca ttccatgtat gtcaggctga aagaaaccat cgcaaggctg gaggatgaaa
    25321 tcgcaaggga acatgagttg gaggaaacac agcgatattt ctttgcggca gcttctcatg
    25381 agctaaaaac gcccatcgcg gctacaagcg ttctgttgga gggaatgctt gaaaatatcg
    25441 gtgactacaa agatcattct aagtatctgc gcgaatgcat caaaatgatg gataggcagg
    25501 gcaaaatcat ttccgaaata ctggagcttg tcagcctgaa tgatgggaga atcgtaccca
    25561 tagctgaacc gttggacata gggcgcacgg ttgccgagtt gctgcccgat tttcaaacct
    25621 tggcagaggc aaacaaccag cggttcgtca cagatattcc agccgggcaa attgtcctgt
    25681 ccgatccgag gctgctccaa aaggcactat ccaatgtcat attgaatgca gttcagaaca
    25741 cgccgcaggg aggcgaggta cggatatgga gtgagcctgg tgctgaaaaa tgccgccttt
    25801 ttgttttgaa catgggcgtt cacattgatg atactgcgct tccaaggctg ttcaccccat
    25861 tctatcgcat tgatcaggcg cgaagcagaa aaagtgggcg aagcggttta ggacttgcca
    25921 tcgtacaaaa aacgctggat gccatgagcc tccagtatgc gctggaaaac acctcggatg
    25981 gcgttttgtt ctggctggat ttaccgctca catcaacatt gtaaatattt aaaacttaaa
    26041 tgattttgac cgacaggtat aaccctgccg gtcttttttt gctgctacag gaaaactaca
    26101 gactggctac agggaaagta cagatgcgct tgccataata acaatcgtac cagccacaaa
    26161 tcgtagtttt attgcaaagg aggcattcga tcaaatggaa aaaagcaact atcattccaa
    26221 tgcggatcat cacaaacggc atatgaaaca atccgtggaa aagcgggctt ttctatgtgc
    26281 gttcattatt tcgttcacag tctgcacgct gtttttgggg tggagactgg cttccgtatt
    26341 ggaggcaaca cagataccgc ccatccctgc aactcataca ggcagcagca ctgacgtagt
    26401 ggagaatttg gaggaaaacg ctcttgccac cgccaaagaa cagggagatg aacaggaatg
    26461 gagcctgatt ttagtgaaca ggcagaaccc catccccgca cagtacgatg tggagcttga
    26521 gcaactatca aatggtgagc ggatagatat tcggatttct ccctatcttc aagatttgtt
    26581 tgatgccgca agaactgatg gagtttaccc gattgtcgca tccggatacc gaacaacaga
    26641 aaaacagcaa gaaattatgg atgaaaaaat tgccgaatat aaggcgaaag gctacacctc
    26701 tgcacaggct aaagcggaag cagaaacttg ggtggccgtg ccgggaacga gcgagcatca
    26761 gcttggtctt gctgtggata tcaatgcgga cggaattcat tcaacaggca acgaggttta
    26821 tagatggctg gatgaaaaca gctatcgttt tggttttatt cgtcgctacc caccagacaa
    26881 gacagagata accggtgtga gcaacgagcc gtggcattac cgatatgttg gcatcgaagc
    26941 tgccacagag atgtacaacc aaggggtttg ccttgaggaa tatttaaaac cagaaaaatg
    27001 agaaaaggat gttatgttat ggacagaaaa agattgacac agcgtttccc gttcctgctt
    27061 ccaatgagac gggcgcagag aaaaatgtgc ttttatgcgg gaatgagatt tgacggctgt
    27121 cgctatgcgc agacgatagg ggaaaaatcg ctttcccatt tgctctttga aacggattgc
    27181 gcattataca accacaatac cggatttgac atgatatacc aggaaaacaa ggtgttcaac
    27241 ttaaagctgg cagcaaagac cttaaacggc ttattgataa gaccggggga aaccttttct
    27301 ttctggtggc tggtacgcca tgcggacaaa gataccccct ataaagacgg ccttacggtg
    27361 accaatggta aactcaccac catgtcgggc ggcggtatgt gccagatgag caatttacta
    27421 ttttggatgt tcctgcatac gccattgaca attatccagc gcagaggtca cgaagtaaag
    27481 gagtttccag agccaaacag tgacgagatt aaaggggtgg atgcaaccat ttcagagggc
    27541 tggattgatt taaaagtgcg aaacgatacc gactgcacct accaaatatg ggtgacctta
    27601 gatgatgaga aaatcatcgg tcaggtgtcc gccgacaaag agccccaagc attatacaaa
    27661 attacaaatg gcagtatcca gtatgtccgt gaaagtggcg ggatttatga atatgcccag
    27721 gttaaacgga tgcaagttgc cttaggtacc ggggaaataa tagattgcaa gctgctttat
    27781 acaaacaaat gcaaaatctg ttatcccctc ccggaaagtg tggatattca ggaggagaac
    27841 caatgagaaa aagtgtgggc attactgttt ttggatgcga acaggatgag gcaaatgctt
    27901 tccgcgcttt atcgccggat tttcatatta tccctacgct gattagcgat gcgatatcgg
    27961 cagacaacgc aaaattggcc gctggcaatc aatgcgttag cgtaggccat aagtccgagg
    28021 tttccgaggc gacaattctt gcgctgagaa aggtcggggt aaaatacatt tctacccgca
    28081 gcatcggctg cgatcacatt gatacgactg ccgccgagag aatgggaatc tcggttggca
    28141 cggttgcgta ttcgccggac agcgttgcgg attatgcttt gatgctgatg ctgatggcca
    28201 tacggggtgc aaaacccacc atgcacgccg tggcgcaaca agatttcaga ttggatcgta
    28261 tccgggggaa agaactgggg gatatgactg tgggagttat tggaaccggc catatcgggc
    28321 aagcggtcgt caaaaggctg cggggatttg gatgccatgt gctggcctat gataacagcc
    28381 gaaaaatgga tgcagattat gtccagcttg atgagcttct aaaaaacagc gatattgtta
    28441 cgctccatgt gccgctttgt gcggataccc gccatctgat cggtcagaag caaattggag
    28501 agatgaagca aggcgcattt ttaatcaaca ccgggcgcgg agcgcttgtc gataccgggg
    28561 cgctggtgga ggcgttggaa agcggaaagc tgggtggtgc ggcactggat gtgttggagg
    28621 gcgaggatca gtttgtttat accgattgct cgcagaaagt gcttgaccat ccctttttgt
    28681 cgcagctttt aaggatgccg aatgtgatca tcacacccca tacggcgtac tacactgagc
    28741 gtgtgctgca ggatactaca gaaaaaacaa tcaggaattg tctcaatttt gaaaggagtt
    28801 tacagcatga atagaataaa agtcgcaatc atcttcggcg gttgctcgga ggaacatgat
    28861 gtgtcggtaa aatccgcaat agaaattgct gcgaacattg atacggaaaa attcgatccg
    28921 cactacatcg gaattacaaa aaacggtgta tggaagctat gcaagaagcc atgtacggaa
    28981 tgggaagccg acagtctccc cgccatactc tccccggata ggaaaacgca tgggctgctt
    29041 gtcatgaaag aaagcgaata cgaaacacgg cgtattgatg tggctttccc ggttttgcat
    29101 ggcaaatgcg gggaggatgg tgcgatacag gggctgtttg tattgtctgg tatcccctat
    29161 gtgggctgtg atattcaaag ctccgcagct tgcatggaca aatcactggc ctacattctt
    29221 acaaaaaatg cgggcatcgc cgttcccgaa tttcaaatga ttgataaagg tgacaagccg
    29281 gaggcgggtg cgcttaccta ccctgtcttt gtgaagccgg cacggtcagg ttcgtccttt
    29341 ggcgtaacca aagtaaacgg tacggaagaa cttaacgctg cgatagaagc ggcaggacaa
    29401 tatgatggaa aaatcttaat tgagcaagcg atttcgggct gtgaggtcgg gtgtgcggtc
    29461 atggggaacg aggatgattt gattgtcggc gaagtggatc aaatccggct gagccacggt
    29521 atcttccgca tccatcagga aaacgagccg gaaaaaggct cagaaaatgc gatgattaca
    29581 gttcccgcag acattccggt cgaggaacga aatcgggtgc aggaaacggc aaagaaagta
    29641 tatcgggtgc ttggatgcag agggcttgcc cgtgttgatc tttttttgca ggaggatggc
    29701 ggcatcgttc taaatgaggt caataccctg cccggtttta catcgtacag ccgctaccca
    29761 cgtatgatgg ccgccgcagg aatcacgctt cctgcactga ttgacagcct gattacattg
    29821 gcgttaaaga ggtgataagt atggaaaatg gttttttgtt tttagatgaa atgttgcatg
    29881 gcgtccgttg ggatgccaag tacgccacat gggataactt cacaggaaaa ccggtggatg
    29941 ggtatgaggt gaatcgcatc atcggcacaa aggccgtggc gtttgctctg cgcgaggcac
    30001 aaatccatgc ggctgcgctt ggctatggct tgcttttatg ggatggatat cggccaagaa
    30061 ctgcggtgga ctgcttcctg cgttgggcag cgcaaccgga ggacaatctc acaaaagaaa
    30121 aattttaccc caatatagag cgagccgagt tgattacaaa gggttatgtg gcctcacaat
    30181 ccagccatag ccgtggaagc gcaattgatc ttacgctcta ccacctggat acaggggaac
    30241 ttgtttcaat gggaagtaac ttcgatttta tggacgaacg gtcgcatcat acagcaaaag
    30301 ggatagggga tgcagaggca caaaatcgaa gatgcttgcg taaaatcatg gaaagcagcg
    30361 gatttcagtc ttatcgcttt gaatggtggc actataagtt gattgatgag ccataccccg
    30421 atacctattt taattttgcc gtttcataat gaaagtattg gttttgtatc agattttgga
    30481 tttgataaaa tctatgctgg tcatggacaa tcttttactg gaaagtggtt aaagcatctt
    30541 ttgtaataca ttgcaaatta tagtattcaa tctgcccagc ggcagaaaag aaaaaaaccg
    30601 ctgttgggca gacctttccc acgcccattt tgtattttgc tggcggcggt tttgagaaat
    30661 gaaccgcccc ctgtcaagta gacagatgaa aaaagaaaaa ttacgccacg agggtctggt
    30721 tcctgtaagc aaggggagcc aggcccttta acttagcttg aagccgcctt gtattgtaaa
    30781 agtctatgta ttcgtcgatg gctttcttca attcctcgaa ggtatgaaat tgccggaggt
    30841 agtacatttc agatttgaga atcccccaga agccctccat agggccgttg tcgatgcagc
    30901 ggccgggccg ggacatactc tgcgttgcat ggataccgtc cagctttgct ttgaactgcc
    30961 ggttggtgta ctgatagccc ctgtcgctgt gaaacagagg atgggcgccg gggttggctc
    31021 tcaccgctct gtcaaaggtg tcaaaaacaa gctgattgtt gttggattgg cccagtgcat
    31081 acgcaacgat gcttttgtca tggagatcaa ggatcgcact caggtatgct ttcttcccat
    31141 tgataagttt gaattctgtg acatctgtca gccatttctc attgggacca tttgccgtaa
    31201 attctctgtt caatacgttt tccgcggtaa cacgcggttc taatatgaca tagtgctttt
    31261 tctttcggcg tatcacggcc tgcatatgta cacttttcat caggcggtaa atccgcttgt
    31321 ggttgtagtt tcttttgagc cggctgttca cattcagcgt catacggcga taaccgtaga
    31381 tgccttttac ttcgctgtac aaacggatga tttcttccag caagagggta ttttcttttt
    31441 ctgaggcact ttcttcccgg tgaagccatt tgtagtaaga acagcgggga atcccggata
    31501 acgcgcataa cgatgcgatt tcatatcctt tttcctgacg aagcgtatgg atcgcctcat
    31561 agaccttcct gcggcgtgtg agcctgttta ccgcctcccc tccagttcgt tcaatttttt
    31621 taagaaatcg ttctccattt gaagccttct gttttccgct tccagctgcc gcagctttgc
    31681 cgcggctttt tcctcctcgc tgtattcgga aggagggcgg cgtctccccc ggcggtcaag
    31741 caacccagct tctccctgct cctgatattt tttgacccag ctatagatct gctgataggt
    31801 gatcttgtac tgttcgctgg tccgccggta gtccagttga tgttctaagc agtatagtac
    31861 tgcctgcagc ttttgctcct gcgataattt ttctttctcg gccatatctg tttcctcccg
    31921 cgctctgcgt gtcttgaact ctctatggcc ttgattatac aacgtaatcc attgttgtaa
    31981 aaccgcatgg gaggaaatcc cgtatttgcg gcacatggca tccagcgagc ctcctccgcc
    32041 catgtagtct tctactgccg ccagcttcag tgaggatgga taacttgtaa catgcttttt
    32101 cggatgcaga cccgcaggcc cctccgtctg atatttccgc agccatgcct ggaaagtgct
    32161 caatcgtatg cctaactctt cccggatctg tgtggaaccc ttacggatcg ctaggtagtc
    32221 ctccacagcc tgaatcttta cctccggcgg tattttgcct tttaaactca tgataattgc
    32281 tccctccgtt tcgacagttt aatttttttg ctgtctactt cagagggagc atatcaaaat
    32341 caaggccgcc gttcttttct ggcagcaaaa ggtgtggcgg cccataggag gatgccgcta
    32401 tgctgttttg ctattgccat gctctaatcc acttccacca ctcaaaaaaa gccttgcccc
    32461 tccacagggc tattccggtt atgagtatgt actataccat gtaaatcagc agcatagcaa
    32521 agcattttct cgcccggtag gtttgcgacc tgccgggcga tttttgtcgt cttttgcggc
    32581 ccggctgtcc ccaaaaaaat tttttgagaa attttcaaaa aggaggtatt tagcgggtag
    32641 ctggcggcct tgtcaaagtc ttgttagcgg aaagggagag aaccacctaa gtcccccaca
    32701 gaaagggggt gagaagatgg atgctatccc ccgcgactat gaagcgcggt gcatgttcga
    32761 tgctttttgc aagacggtac tgcgtaacga ggccaaaagc tacttggctg aaatgaagcg
    32821 ccgccgtgac cgtgaagtgt cactaagctc tttgtcacag gcagatttgg acaagctctg
    32881 tacagtagat cactacccca gcgacagctt tacgttttcg tcgcatggct atgacctaca
    32941 tatcaacaat gagcttgttg ccgaagcctt tgccgcgctg cccagcatgg agcagagtat
    33001 tttaatcctg cactgtgttc tggatatggc agatggtgag atcggcggcc ttgtgggaat
    33061 gtcccggagt gccgttcagc ggcacaggac aaatacctta tcggagctgc ggaaacagct
    33121 aaaggcgttg atgccgaagg gaggttagac gcggatgaag aacccgaact acaagggcat
    33181 tgagcttttg cccctttcgg tgatcgaggc cgcccgcgct ggggatgccg gagccgtaga
    33241 gcgcgttttg cagtattaca gcggttacat gaacaagctg tgtacccgga cgctctatga
    33301 cgaaaacggc tatccgcatg tgtgtctgga cgaggccatg aagcgccgtc tggaaatcaa
    33361 gctcatccac gctatcgtca cgaactgacg aaccggcccc ggcagcatga aacggcttac
    33421 cctttccccg tttcggtgtt ttcccggtgg cctgatcgca ccttgataaa tatgcccgca
    33481 agataacggc ggcgcatcat agggcatacg gacacattct gtctgtgccg agccggacgg
    33541 ctggtgcgcc atgatacttt tccctacggg aaaagcggag cgaggaacac cacgccgcaa
    33601 tgtgagcgtg gcggcttgcg ggcgacgacg tacaggcagg actatgatac tcccttatag
    33661 ccacagtccg agcgttaaaa gcgtcgcagg caatgggtag ggccgcgtga gaaccacgcg
    33721 ggggtgaaag tcccgtggag ctgtgccagc agccgtccgt ctatgcccct aaatcacaca
    33781 aacctttgaa tttgtgaaag ggggctgcat tatgaaccat gatgtgccta tttgggaaaa
    33841 atacacgctg acgattgagg aagcgtctaa atatttccgt atcggagaaa acaaattgcg
    33901 taagctggcc gaggaaaatc cggcctctgg ctgggtcatt atgaatggca accgcatcca
    33961 gatcaaacgc aagcagtttg agaaaataat tgacactttg gacacaatct aatagtgaaa
    34021 aagggccgca gatatgttat aataatttat aagcatatat gcggctcttt ccgtcatgga
    34081 aaggagcatg acgagatgtc agaaaaaaga cgagacagca aaaatcgcgt tttgcggtct
    34141 ggagagagcc agagaaaaga cggacgatat gcttacaaat atgttgatac ctttggaaaa
    34201 ccgcagtttg tttactcatg gaagttagtg cctacggata agaccccggc aggtaaacgg
    34261 gaggatattt cactccggga aaaggagaaa gaaatccaga aagaccttga cgacgggata
    34321 gacaccatcg gcaagaaaat gaccgtctgc cagctctacg ccaagcagat caggcaccgg
    34381 ggcaatgtga agcacaacac caaactgggc cgggaacggc tgatgcgtat tctggaacaa
    34441 gatcggctgg ggagctgccc gattgacagt gtgaagctgt ccgacgcaaa ggaatgggcg
    34501 cttcggatga aagaaaaggg cctttcttat aagaccatca ataacgacaa gcgttccctg
    34561 aaagcggctt tttacaccgc catacaggac gattgcatcc gaaaaaaccc gtttgacttc
    34621 cagcttagtg acgttctgga cgacgatacg gaaccaaagg tgcctcttac accagcacag
    34681 gaagaaagtt ttctgtcctt tattcaaggt gataaggttt accaaaagca ttatgacgca
    34741 atcgttattc tgctggggac agggcttcgc atttcggaac tgtgcggact gacggacaaa
    34801 gatttggatt ttgaaaaccg ggtaatcatc gtgagccacc agcttttgag aaataccggc
    34861 gtcggttact atattgacga acccaagacc caaagcggcg tcagaaaaat ccccatgaat
    34921 gaggaagtgt atcaggcgtt ccagcgagtg ataaaaaacc gcaaaggtgc aaagccgttc
    34981 attattgacg ggtatgccaa tttcctgttt ctcaaacaga acggctatcc catgacggcg
    35041 gtagattatg gcgggatgtt tggcaggctt gtgaagaagt acaacaagag ccatgaagaa
    35101 gcgttgccga aaacgacgac gccccatgct atgcgtcata cattctgcac ccggttagcc
    35161 aatgcgggaa tgaaccccaa agccttgcag tacattatgg gacattccaa tatcaccatg
    35221 acgctgaact attatgcaca cgccacattc gattccgcaa gggcagaaat ggaacggctg
    35281 gcagcctgac acaaggccgg ttttactact ccatttacta cttttgaggg ggaaatggtg
    35341 agaggtaata agaacatttg taagtatcta acaatatcaa aaatgccgga aaagcccgga
    35401 aacacgggct ataccgacat tcaagaactt ctaaaaagat aatctaaaat cccatataat
    35461 tt
//
LOCUS       DAZUKC010000279        12925 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.guided.071, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000279 DAZUKC010000000
VERSION     DAZUKC010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.guided.071"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            179..1075
                     /locus_tag="ACG80T_002988"
     CDS             179..1075
                     /locus_tag="ACG80T_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFS4842539.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1225..2166)
                     /locus_tag="ACG80T_002989"
     CDS             complement(1225..2166)
                     /locus_tag="ACG80T_002989"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFS4842540.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2292..3412
                     /locus_tag="ACG80T_002990"
                     /pseudo
     CDS             2292..3412
                     /locus_tag="ACG80T_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            3670..5172
                     /gene="eat(A)"
                     /locus_tag="ACG80T_002991"
     CDS             3670..5172
                     /gene="eat(A)"
                     /locus_tag="ACG80T_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFS4842541.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5239..5766
                     /locus_tag="ACG80T_002992"
     CDS             5239..5766
                     /locus_tag="ACG80T_002992"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS4842542.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            5977..7038
                     /locus_tag="ACG80T_002993"
     CDS             5977..7038
                     /locus_tag="ACG80T_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFS4842543.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            7041..7937
                     /gene="murQ"
                     /locus_tag="ACG80T_002994"
     CDS             7041..7937
                     /gene="murQ"
                     /locus_tag="ACG80T_002994"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFS4842544.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7951..9933
                     /locus_tag="ACG80T_002995"
     CDS             7951..9933
                     /locus_tag="ACG80T_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFS4842545.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9998..10444)
                     /gene="nrdI"
                     /locus_tag="ACG80T_002996"
     CDS             complement(9998..10444)
                     /gene="nrdI"
                     /locus_tag="ACG80T_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFS4842546.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            10655..12079
                     /locus_tag="ACG80T_002997"
     CDS             10655..12079
                     /locus_tag="ACG80T_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFS4842547.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(12260..>12925)
                     /locus_tag="ACG80T_002998"
     CDS             complement(12260..>12925)
                     /locus_tag="ACG80T_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS4842548.1"
                     /translation="KKYTTIIDPVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTL
                     KNLLYRKYSRGEILVSKWITLVIYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQT
                     LIQSLFTYTLGSYIGLWLILSLVLMIACFINSSGASISAGIVFYFASSIISGILIALI
                     QKWEWIKWNPISMLNLQNQIGNEEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKR
                     KNV"
BASE COUNT         4151 a         2266 c         2795 g         3713 t
ORIGIN      
        1 gacaattaca actattcaga aaacttttag gcgaatttta atagaagaat gatttatttc
       61 atcatatttt catacaaatg ttaaattttc atacaaatat aggaaacgtt ttactggctt
      121 tttactattt aaacaggcta tcataggaaa aagtgacaga gaaagtgagg cggaaaagat
      181 gaacaaaaat gacgtactgc tagttgcaaa aaataaatca ctttattttt tgccttccat
      241 ccgctcttta gatgtttggt ccggaaaaga tttactacag tcggaattca ttaaatcagc
      301 tagacaacag ttggcaaatt atcagctgat cgttttttta gattatggat ttgatatgga
      361 gatggctcga cgcgtgcgcc catttaccaa tgcaaaaatt gtgttatttt tttggaatca
      421 cttcaaagca gaacacgagg cgatgctgaa gcaggcacaa gatgaaccgg caatcgatga
      481 gatttatcat tttgattttt tggaagcaaa agaacttggg ctgaaacata atagttcatt
      541 ttattcgaaa catatgaatt tttcttctag cgaaccaaag acggatctat tttttggtgc
      601 aacgaataat gggagaaaag aacgagcaga aagctataag caggaattta taaaaagaga
      661 tgttacgacc aactatttca ttcttccaca tcgagggaat gaacaacctg gatatcttac
      721 ttatgaagag tacctgaaaa aaacagggga atcgcgcggg attctggaac tgttgcggga
      781 gggtcagcaa ggagtgactt tgcggacatt tgaatccatg tattttcaaa agaaactagt
      841 gacggataat caagcgattg ctcattatca tttttatcgt cctgaaaata tttttttgct
      901 tcgagaacgt gatttagatg aattaccgga atttctgcat acaccattta agccaatcct
      961 atcagagcta ttggaatttt atgatgcaga aaattgggca aaacgattta tcactaacga
     1021 ttccactatt tatgagcaat acgaatatga cctctcctct gttgaaaaca aataaaaaac
     1081 ctacattttg aagagattat gtactgaaac aaacaagcac aattcttatg gcatcatgag
     1141 agttgtgttt tctttttgat cttttattgg gcttaaaaaa agagaaccag ctgctgattc
     1201 agctgattct catcagagag ccttttattc tgcgccaagg attccttctt ttgcatggac
     1261 taattgggtc aaaaaggcac aatatggtgt agcaatatca tatttttgtc cttttcgcca
     1321 aatggcacca ttgatgtagt cgatttcagt caaacgatgg tttttgatca ggtcttggta
     1381 catagatggg taatgcaacc cgatgttgtc aggatcataa gtagaagcaa tatgttggta
     1441 cacttcttct tgatctagta tgattccttc ttttgctgcg atagaagcaa attcagatac
     1501 gattgttcga accatgctct ctgccgcttt ttgcgcgcct agttcagcaa tattacaatc
     1561 caaaatcgtg cataaaccat tcaatgttcc atttacacaa gcttttcgcc aaatggaata
     1621 acgaacgtta tggctataga tcggatttaa atttgcttct tgaaagactt ctacaacttt
     1681 ttttgtaaat gcttcgcctt ctggttccaa gttctctagt tcgatttctc catcacctaa
     1741 caatgtgact tttccgggac cagctagacc agctgtccac atcgtgatcc caaataagat
     1801 atttttcttt ggcacatatt tttccagtac atcttcatga cctaatccat ttagtaaaca
     1861 caaaacataa gtattgtcag taataatgga ttggatcgaa caaaacatat catctaattg
     1921 attggctttt gttaatgcta cgatcaagtc cacgtgttcg ttgctttcga tgatttcttc
     1981 tggtgaatag attggcagtt ttgcgaccac ttctttaccg ttaaagtcag caatcaaccc
     2041 atttgtacga atggcttcaa tgtgagcagg ccaacgatcg atcaagagga cctcatttcc
     2101 gctttgatgt agcatcaatc cgattcggct gcccattgct ccggccccag ctattgcgat
     2161 tttcatgttt tttctctcct ttattcaatg agttcccttt tagtatatag aaaaatggaa
     2221 gaaaatagca ttgttttgtt tgaaaaaaat gaaaaaaagt gtcttactta agaagaaagt
     2281 gagggataca gatggatatt ttggcggcaa ttgattcatt taaaggaagt gcttcttcat
     2341 ttgaactaaa tcaggcagtt ctaaatggct gggaagatcg taaaggaaga aaaatcaatg
     2401 tgccgattgc agatggcgga gaaaatactg cagaagcaat ctattacgcg ctcaggggaa
     2461 catggcgtac cattgatggg atggatctct tattccgtca gcgaaaaatc agctatttat
     2521 tgacaacttg gcaaggaaaa aacaccgcag tcatcgaatc tgcacaagca ttggggttag
     2581 atctacttga taaaccaacg gatcaaacta tccgacttgc ttccagttat gccttaggag
     2641 aacttattaa agatgcactg cttcaagaag tagaacaaat tattgtcact ttaggaggaa
     2701 gcgggtgtag cgatggtggt ctaggattac tccaatcctt gggtgcttct ttagaagggt
     2761 acacagaagg aaatccttta ttatctacta aaaaacttca ttttgatcga agaaacgttc
     2821 ttgtttctaa agtaaaattg attatcgctg cagatgttac caatccatat acaggtcaac
     2881 aaggagctgc ttggcaattt ggaagacaaa aaggaggaat agatacgacg ctagctttct
     2941 tagataaaca agcaggaaag attgctcagc aaataaaaga tttctgggag gtggatttag
     3001 atgatattcc aggaagtgga gcagcaggcg gtattggtgg agcccttttt tgttgggagc
     3061 gaagatggtt tctggtttcg atttagtcag ccaaatcact ggattggaaa aaaagatcga
     3121 acaagcagat gtcatcatta caggagaagg gcggatcgat cagcaaacga tccacggaaa
     3181 agttccatac ggtgtagcag tgcttgcaaa aaaatatcga aaaccaataa tcgctatttg
     3241 cggaagtaga gaccaagagc tagaaggact ttctgatttt ctcccaattg tcttaagtat
     3301 tcatttgggt ccaataggtt tagaagaagc aatggaacac aggcaaacat tagaaaaaac
     3361 aaaaatctta ggacagacac ttagcagatt tttcaccatc ctcccgaagt agttgacaaa
     3421 atcattttct ttatgctatc gtaatttcaa atataggaca aagtgaggga tccgtgcatt
     3481 gaaaaattaa gtcgattgat gaaagctgca ttcagactgt accttgtgga gacaaggaag
     3541 cagtgtgcag attttcggga ttggcttggt tttttcgatg tatgggtctt tttgtgtgta
     3601 tccaaccctt gcatcctctt tagccagttc tgaaaatagg actggtcttt ttttacgagg
     3661 tgatggaaaa tgtctaaaat cgaaataaaa aatctgacat tcggctacga cagccaaggc
     3721 acattattat ttgaacaagc aaatctaaat tttgacacac aatggaaact aggacttatc
     3781 ggacgaaacg gtcgaggaaa gacaacttta ctgaatattc tacaaaacaa actaccttat
     3841 caagggcaag taatccatca gcaagaattt gcctatttcc cgcaacagac aaaagataaa
     3901 gaacgtttaa cctattacgt gttaaatgat attacggatt ttgagatatg ggaaatcgaa
     3961 agagagctcc aattgatgca aacagatcct gaaatcttat ggagagaatt cagcacacta
     4021 tcggggggag agaagacaaa agtcctactg gcacttttat ttgtggatga cactcatttc
     4081 ccgttaatcg atgaaccaac gaatcatttg gatatctctg gtagaaaaca agtagcggct
     4141 tatttgaaaa agaaaaaaca aggcttcatc gtggtcagcc atgaccgggg atttatcgat
     4201 gaagtagtgg accatgtttt agcaatcgaa aaaagtcaac tggaacttta tcaagggaat
     4261 ttctctatct atgaagaaca gaaaaaactt cgtgatgaat ttgaaatggc tcaaaatgaa
     4321 aaattgaaaa aagaagtcag taggctaaag aaaacagcag ctgaaaaagc cgaatggtct
     4381 cgttcccgag aaggagataa aacaaagaaa caagtcggat tcatcgatac tgaatctaga
     4441 cgagtgaata aaggagcagt gggtgctgat gctgcacgga cgatgaaacg atccaaagca
     4501 atcgtgaatc ggatggagac ccagatcagc gagaaagaaa aactattaaa agatatcgaa
     4561 tatatcgatt ctttgacgat gaatagccaa gcgtctcacc ataagcgact tttaagcgta
     4621 gaagatcttc aattagggta tgaaaatctg ttattcgagc caattcattt tacaatcgag
     4681 cctcatcagc gggtggcgat ttcaggtcct aacggtgcag gaaagtcatc cattatccat
     4741 tatcttctgg gggcattcaa cggcaaggtt ataggagaaa aataccagcc aaaacatctg
     4801 agcattagtt atgcaagcca aaattatgaa gacaatcgag gaacgttggc ggaatttgca
     4861 gagaaaaacc aagtagacta ccaagcattt ttgaacaacc tccgaaagct tgggatggaa
     4921 agagatgttt ttcataacaa gatcgagcag atgagtatgg gccaacggaa aaaagtggaa
     4981 ttggctaaat ctttatcaca gccagctgaa ctatatacat gggatgaacc attgaattat
     5041 ttggatgtct tcaatcaaga acaattagaa caactgatct tgaacgtgaa acctgccatg
     5101 ttactagtgg aacatgatca aaccttcctg gataaagtat ctactgagat tatttctctt
     5161 gagagaatct aacccttgta atctcaagaa caacaaggta tggttaaggg gaactttgcc
     5221 aacgaaggag agaatcacat gctttatgta acgagacacg gagaaacaac ttggaacgca
     5281 caaggattag tttgcggacg ggcagatgtg ccgctgactg aaaaaggaca gatgcaggca
     5341 cagaaactag cagaaaaggt agtagatttg cctgttccaa tcacgaaaat catccattcc
     5401 cctcttcaac gagcaagaga tacagcacaa gcagttgctg atcgtctcag cttgccgctg
     5461 accgttgatg aacgattggt agaaatggat tttggggatt atgatggact gccaagcaaa
     5521 gatgagaact ttcaaaaagc acgtttagcg ttcgctgtac gattcccaaa tggggagtca
     5581 gtcttagatg tgtatgcacg gatcgttcct ttattgaaag aatgcatcga agacgaagaa
     5641 aatgtctatc tgttagtttg ccataatgcc ttgataagag tgatcaatgc ttattttcat
     5701 ccgatgccta atgaggggtt ctttacattc atggtagata atacagaatt ggtttcttat
     5761 gagtaattat gtttttgtgt tatttaaata aaatatttca aggcactgct tttgcttagc
     5821 agtgcctttt ttaggttatg acaattcttt tgataatata aactcaacct ttagaaatga
     5881 aatttaatta ttttaataaa agtattgaaa ttttgtttct ttagatatat actctagtca
     5941 tagaaatgaa gacggataca gaaaaagagg gataatatgt ttggcttttc aatatttatg
     6001 aacgattctt tgtcagaaga gaagaaacag tatatccgcc aaatggcatc caatggcttt
     6061 gtcggtattt tcacttccat gcatatacca gaagatgatg tttcggcata tcaaaagcga
     6121 ttgatcgaac taggaagcga agcgaaaaag aataaactag aattgatggt cgatatttca
     6181 ggagaggcat tggaacgagc gggtttttca ttagaagatc taacgccttt gaaagcaatt
     6241 ggtgtaacag gtttaagaat ggattatcat atttccaatc agcagattgc cgactggtca
     6301 catcaattga aaatcagttt gaatgctagc acgatcacgc ctaaagatat cgatgaattg
     6361 aaagaagcag aagctgattt ctcacagatt gaagcttggc ataattatta tccacgtccc
     6421 gaaacagggc tggacaaaga atggtatcag aaaaaaaatc aatggctcaa aattcaaggt
     6481 ctccttgtcc aaggttttgt accaggagat acagagttga gaggcccact atatcaagga
     6541 ttgccaactt tggaagaaca tcgagggatc catcctcttg caggcgctct agatctatta
     6601 gcaagtaaca cggatattgt ttacatcgga gatgcaggtc tgtcagaaaa cgttcaggag
     6661 caatttgctt ctttccaaaa agaacagaca gtcttgctcc atacggaacc tgtagatgaa
     6721 gaattttacg aatatatatt agggaagcat acgaatcggc aagacgatgc aagagatgtc
     6781 atacgaagcg tagatgcacg ttttcgagag attccgccta tcccagcaag aaatacagct
     6841 acgcgtatga aagggtctat tactttagat aacgaaaagt atttgcgcta tatgggtgag
     6901 atccaattaa ccaaatatga tttgccagcg gatgaaaaag tgaatgtagt agctaaagtt
     6961 atcaaagaag agctaccatt gatcaaccaa atcaaagctg gtatgaacta tcaatttata
     7021 agaaaagaag gaagataaaa atgagcaaaa tcaatttaga gaacctaaca acggaacgca
     7081 gaaaccaaga aacttttgga ttagatgaga tgagtatttc tcaagccgtt caactaatga
     7141 acaaagaaga tcagaaagtg gccgctgcgg ttgaaaaaga actgacaagt atcgaaccag
     7201 tgattgctaa cacgattgag tcctttaaaa aaggtggacg tctgatttat atgggagccg
     7261 gaacaagtgg aagactaggt gtgttagatg ctgctgaatg cgtaccgaca ttcggtgtag
     7321 aaccggaaat ggtcataggt ctaattgctg gtggagaaga agcaatgact gtagctgtcg
     7381 aaggggccga agattcttat gagctagggc aagaagatct aatcaagctg aatttgacaa
     7441 aaaatgatat agttattggt atcgcagcta gcggtcgtac accatatgtg atcggtggat
     7501 tagattacgc aaaatcgatc ggtgctgtca caggaacgat ttcttgcaat aaacaagccg
     7561 aaatcagtaa acacgcggat ttcccaatcg aagtcgatgc aggtccagaa tttttgacag
     7621 gctctactcg attgaagtca ggaacagcac aaaaacttat cttgaatatg atttctacta
     7681 tcagtatgat tggtataggt aaagtattca ataacttgat ggtcgatgtc aaaccaacaa
     7741 atgaaaaact aatcgaacgc tcaaaacgaa tcatcatgca agcgacagaa gctgattacg
     7801 aaacagctga aaagtacttt gaagaagcag atcaaaatgt taagctggct atcgtcatga
     7861 ttttgacaaa tagtgataaa gaggaagctc aagcaaaatt agctgaagca aacggatttg
     7921 taaaagggac cttatgaggg ggaaaagacc atggcaaaaa acgaactgac tttggaagaa
     7981 ctggcaaaac aaatttataa aggggcaggc ggcatgggca atgtagaaag cattgtccac
     8041 tgcatgactc gtgtgcgtat gaagatcatc aatgatgatc ttgtaaatgt ctctgaatta
     8101 aaagcgattc caggagtgct tggtgtagta gaagatgaac agcttcaagt catcatcgga
     8161 cctgggaaag tgaacaaagt agcacagcaa atggttgatt tggcaggcgt gaaattagga
     8221 gaaaaacttt cttctgtaag tacagctaca gcaaattcat cagcatctgg aaaagaccgt
     8281 gtaaacgaac gggcacaaga aatgaaagcg gcacaaaaat cgaaacaaaa accatctaaa
     8341 atcaaatcgg tattaaaaga catttctaat atctttgtgc caatgattcc tgcatttgtc
     8401 ggtactggga tcgtggcagg gattgccgca gttttagcaa acttagtcac agccggaaca
     8461 cttgatgcag caacatggca acaatatatc gatgtgatga acatcttgaa aaacgggatg
     8521 tttagctacc tagttatcta tacaggtatc aatgctgcac aagtttttgg tgcaacacca
     8581 actcttggtg gagttatcgg ggccgttgtg atgctgacag ggatgaatcc ggaagcacca
     8641 ctgaaaaact tatttactgg tgaagctctt gcagctggac aaggtgggat cctaggtgtc
     8701 atcttcgctg tttggctatt gtcaatcgtt gagaaaaaac tgcataaagt agtacctgat
     8761 gcggtagata tcatcgttac accaactctt tcattgattg ccatcggtct gattgaaatt
     8821 ttcttcatca tgcctttagc aggtttcatt tctgatggca tggtcggtgg gatcaactgg
     8881 gtacttggcg ttggtggagc atttgccggc ttcgttctag gcacattatt cttgccgatg
     8941 gttatgttcg gactgcatca aattttgact ccaatccatg tgcaaatgat tgatgaaact
     9001 ggtcgtacgc tattgcttcc aattcttgcg atggctggtg caggacaagt gggtgcagca
     9061 ttggcattat ggattcgctg caaaaaagat aaagaattaa cagaaatgat caaaggagct
     9121 ttaccagtcg ggatcctagg aatcggcgaa ccattgattt acggagtaac attacctcta
     9181 ggacgcccat ttattactgc ttgtatcggt ggaggtatcg gcggggcagt gatcggaatg
     9241 atcggaaatg tcggggctat cgctattgga ccttcaggag ccgctttgat cccattgatc
     9301 tctgacggta aatggttagg ctacatcctt ggattattgg cagcttatgc tggtggcttc
     9361 gttgctacat tcttcttcgg tattcctaaa gaccgtttag aaaaagaaga attagcgact
     9421 gaaacagcca cagtaaatga agtgcctgaa aaagtagtac ctcaaacttc agctgaaagt
     9481 gtgactatcc tctcatcagt tgctgacgga acagttgttt ctttagataa agcaagtgat
     9541 ccagtttttg cacaaaagat gatgggtgaa ggctactttg tagaaccaga aaacggacat
     9601 atctattctc cagttactgg ggtagtcagc tctgtcttcc caacgaaaca cgctattggg
     9661 atcacaacgc caaatggttt agaaatcttg cttcatatgg gaatcaacac agttgactta
     9721 ggcggtacgc catttgatgt gaaagtaaca gaagggcagc aagtgacacc tgatacgctt
     9781 gtagcagaag ctgacttaga agcgatcaaa gcagcaggca aagaaacatc catgatcgtc
     9841 ttggtaacca atatggatcg tgtaaaaaac tttgtcttgg aaaaaacagg taaagcaaaa
     9901 gcaaaagcac ctgtgatgga tgtcaaagct taatatgata gaaacaatag aaacgaaaga
     9961 agaaactgaa atctattttt cagcttcttc ttttttatta tttattgttt tctgctaaaa
    10021 tctgataaat tcgtgtcaca tcagatggtg ttccccgtaa ttcataatct gccaagaaag
    10081 gaaagccaaa tgtttgtgca tattgtttgg ctgttagaca atactgatta ttgaagtttt
    10141 tatttccgct accgactaca ccaagacaaa gtttatgatt gtcttcatgt tccagatatt
    10201 cacgcatcgt ttcggtcaag atttctgtat caccgttatc taaaccattt ccgccgtcta
    10261 aataagtagg aacgaaagta aaaaatggtt ctgtttcttt cgtaaaatca ctattttcat
    10321 ggatttcttt taaagtaact tcaggattga caggatcttc tgcatgcatt ttttcagcat
    10381 attctgctag atgtttggca aaggcacgcg tatttcctga aatcgatata tataaaatgt
    10441 tcatggatca tcctccaatt ctttcctttt agtatagcat cttttttaag ttttttggtg
    10501 gtgtaatttg gaaagggact aggcgctttg cttgtataat gagaaaaaag ccggtagaat
    10561 tatatttcta ttaaagtttt tattaattaa gtaaaaataa aaaaggtttg ttatactttt
    10621 tgataaaagc ggttttattt tggaggatgc ttacatggat acagagcaac agaaaaaagg
    10681 aattggttta attccacttg ctgccttagt aattggttca gcgattggag gtggcgtatt
    10741 tggtattatg acggacatct ccggatcggc tggcggccca gctattttca gttgggtact
    10801 cgtaggaaca gcaatgttga tgctctcctt gtcgatcaat aatatcaata aaaaacgtcc
    10861 agatttaaaa ggcgggatct ttagttacgc agaagaaggt tttggccgtt tcgctggttt
    10921 tatcagcggt tggggttact ggctcacatg ctggttaggc aacgtagctt ttgcaacatt
    10981 gttgatgagc gcattaggat atttcttccc agtatttgaa ggtgggcaaa atctggcatc
    11041 gatcgtgatg agtacagtct ttttatgggg atatgcatgg ctagtgaatc ggggagtcga
    11101 aaatgctagt ttcgtcaatg ctatcgtcac attctgtaaa ttagtgccat tatttttatt
    11161 tattgtagta gctatcagca cgttttcgat ccaacaattc attgataact tttctagcga
    11221 cattatattg aatgcgaatg gacagccgat cccgttttcc agtcaagtat tgagttgcat
    11281 catggtgatg ctttgggtat ttgttggtat tgaaggagcc tctgtcgtag gttcacgagc
    11341 aaaaaaacaa tcagatgttg ggaaagcaac gattctaggg atattcggct tgattattat
    11401 ttacgtcttg gtttccatgc ttccatatgg gacaatgacg atgaatcagc tgcaaacgat
    11461 caaggcgcag cctgctatgg gctatgtgtt tgaaatgatg gttggtaaat ggggagcagt
    11521 cgtgatcaac ggcggtttga tcatttcttt agttggtgtt tggctctcat ggacgatctt
    11581 accgattgaa acaatgggaa acatggctga agacggactt ttgcctaaag catggggaaa
    11641 actcaacaaa aatggggcac caacttatgc tatcgtattg acgacgttat gtacgaatgt
    11701 ctttttactt tcattattat tcactgacag tgcttataac tttgcttaca cacttggtac
    11761 agcagctatt ttcttcacgt ggcttttctt aggattgtac caaatgaaac tttcttatca
    11821 gcggaaagaa tgggtccaat ttttagtagg gcttttagca agtgcctttc aagtatgggc
    11881 tatgctagtc gtagccttta atgaagtaat gcttgttctt gttcttttct tgccaggcat
    11941 ttatttttac ctcaaagcac gtaaggagca aaacgagcct gtacaacata tccacttaga
    12001 taagatttta cttataggtg tgacgatggt cggagtgatt gcaatggttt tatttgccac
    12061 tgggatgctt cacgtataga agtataaaag aagtataaag agaggttgtg agcctgtcgt
    12121 ttattcaaaa acatactagg aaaggagctg tgacacaaat gttgtcacag ctcctttttc
    12181 agaataaacg gtgtttaaga gacagccaat cggcaataag gctgaacgtc tctttcacag
    12241 cctctttttt gatgagtcat catacatttt ttcgcttaaa tacgagatat cctaatgcta
    12301 agaacaaaac gatgtaagct aaattaccga atagcatttg attagtagaa agatgagtca
    12361 attgcttcat gatctcttcg tttccaattt ggttttgcag attcaacata ctgatcggat
    12421 tccatttgat ccactcccat ttttggatga gtgcaataag gataccagat atgattgacg
    12481 aagcaaaata aaagacgatt cctgctgaga tggatgcgcc ggaactattg ataaagcaag
    12541 caatcatcaa cactaaactt aaaatcagcc ataaaccgat ataactaccc aaagtataag
    12601 tgaacaagct ttggatcagt gtttgaccag tagaaacctt ctccgtgaag gaaatcgaag
    12661 ggaaaagaat cagtttcatc aacacagtga caataatagc taaaaggtat aaataaacag
    12721 aataaataac taatgtgatc catttgctta ctaatatttc tcctcgagaa tactttcgat
    12781 aaaggagatt tttcaatgta ccatattgtg cttccatcga aatgatcgtg cttgcagctg
    12841 caatcatgat aaagacaatc caagatgtgg cactaaaaag ctgagcagtc atttcaactg
    12901 gatcaatgat cgtcgtgtat ttttt
//
LOCUS       DAZUKC010000280         6676 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.guided.121, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000280 DAZUKC010000000
VERSION     DAZUKC010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.guided.121"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            117..782
                     /locus_tag="ACG80T_002999"
                     /pseudo
     CDS             117..782
                     /locus_tag="ACG80T_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            818..1063
                     /locus_tag="ACG80T_003000"
     CDS             818..1063
                     /locus_tag="ACG80T_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS4842549.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            1166..2035
                     /locus_tag="ACG80T_003001"
     CDS             1166..2035
                     /locus_tag="ACG80T_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFS4842550.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLLECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            2016..2750
                     /locus_tag="ACG80T_003002"
     CDS             2016..2750
                     /locus_tag="ACG80T_003002"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4842551.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            2783..3691
                     /locus_tag="ACG80T_003003"
     CDS             2783..3691
                     /locus_tag="ACG80T_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFS4842552.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            3688..4230
                     /gene="sat4"
                     /locus_tag="ACG80T_003004"
     CDS             3688..4230
                     /gene="sat4"
                     /locus_tag="ACG80T_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFS4842553.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            4323..5117
                     /locus_tag="ACG80T_003005"
                     /note="aph(3')-IIIa"
     CDS             4323..5117
                     /locus_tag="ACG80T_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFS4842554.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            5393..5524
                     /locus_tag="ACG80T_003006"
                     /pseudo
     CDS             5393..5524
                     /locus_tag="ACG80T_003006"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            5600..6345
                     /locus_tag="ACG80T_003007"
                     /pseudo
     CDS             5600..6345
                     /locus_tag="ACG80T_003007"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            6456..6635
                     /locus_tag="ACG80T_003008"
                     /pseudo
     CDS             6456..6635
                     /locus_tag="ACG80T_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibD"
BASE COUNT         2287 a         1005 c         1461 g         1923 t
ORIGIN      
        1 tttccattta gatagtagtg gaaagacctc tatttatctt ttgaaagtga atacaaaaat
       61 aaagcacact ctgctataat ataggtgatt aatccatttg agagaggtgc tttatcatgt
      121 atcaaaatta taccataaac caactttgtt tgcctataga tcttgaaatt aaattagaag
      181 aaaatgattt tgctcatgcc attgtgcagt ttgttgactc tattcccgat gaagtctttc
      241 ttccttatta tcaatctatg ggtagacccc aatatcatcc tcgtatgcta ctttccatta
      301 ttctttgtgc ctatattcaa ggggtttatt cgggccgcca aatacaaaat atgctgatag
      361 atagtattcg gatgcgctac ttatctcaag agcaattccc taactttcgt actattaatc
      421 gttttcgcgt tcatccgatt atgaacgata ttctagatca ttcatttgtt caatttcgtg
      481 agctactggt tcaaagtggc ttaatcagtg gcagtgctct atacattgat ggtacaaaaa
      541 ttgaagcgga tgcgaataaa tactctttcg tttggaaaaa aagtatttta aagtataagg
      601 atagccttga tcaaaaagcc cttgaaaact accaacaaat ggtggaaaca aaaatcttac
      661 ctgagttaat agatgattta aaggatgaac tttctataga agagattgaa aggattcgtc
      721 aatctttgga agaaaaggaa aatcaattaa ttgaagcgat tgaccaaacc gaaacagtag
      781 aagggggctg acccattatt aataaggagt ggacaacatg attgatgtaa atgagctatt
      841 ggaagaagct attagagaaa ctgaaaatct aaatgatggc gaagtttttc ttgttaaaga
      901 tttattcaag ggatatgtgg ggaataggat acccagaaag gatcgacttc tgcttggaac
      961 gttattttta aaccatgtta gcaaaatgaa tggtaattta aaggctattg aaaagacttc
     1021 atctaatcag cagaggtata aaaagacaat tgataagttt taactatctt ttgtgaaatt
     1081 aaaaaaatta taaatatgtt gagtagaagc aaaagatttg gcttggaatt tttacagcag
     1141 tgaatcgata aaaggagcat gcttgatggt agataatatt attaaatcag tagcagagaa
     1201 attatcctct ctgtcttata tagaaggtat tgttttaggt ggttcacgtg caaggggcac
     1261 ccatacagag gattcggata tagatatcgg catctattac aattcagaat catttgacat
     1321 aaatactatt aatcaattcg ctacaaagct ggatgatgag catagaaata accttgttgt
     1381 acctcccgga gcatggggtg attggattaa tggcggcgga tggttagtca taaacgggta
     1441 tcatgtggat ttaattttac gtgatattaa acgtgtggaa caaataatga aagatacaga
     1501 gcacggaatt gttactgcca attatcagac tgggcatccc catggttata ttagtgcaat
     1561 gtatcgagga gaattagcga ttagcaaaat actatatgct aagaatgaaa gcttatgcga
     1621 attaaaaaaa caggcagaaa cttatcccaa tgctttgcag aaaagtttag ttaacttttt
     1681 tatgtttgaa gcagggttct ctttaatgtt tgtaaaagca aattcgggaa cagacgataa
     1741 atattatatt gcgggtcatg tttttcgtat agtttcatgt ttaaatcaag tgttatttgc
     1801 atgtaataat gcttattgta tcaacgaaaa gaaagctata aaactgcttg aaacttttga
     1861 acataaacct gaaaaatata ccgagaaggt aaatcatatt tttgaagtac tcggtatctc
     1921 acttttggaa tgctacgaca tgaccgagaa gctttataat gaagtgaatg aaattgtatc
     1981 ggagataaat aactttttaa acgaggagag ttcagatgaa agaaaacaaa tatgatgata
     2041 atatattttt tcaaaaatac agtcaaatga gtcgctcgca gaaaggactg gctggtgcgg
     2101 gagaatggga gactttgaaa aagatgctac ctgattttaa gggtaagcgt gtgcttgatt
     2161 taggatgcgg ctatggatgg cactgtatat atgcgatgga aaacggtgct tcctctgtag
     2221 taggtgttga tatttctcat aaaatgctcg aagtagcaaa aggaaaaacc cattttccac
     2281 agattgaata tgaatgctgt gccatagaag atgtggattt cccagaggag agctttgatg
     2341 taatactaag ttcgcttgcg tttcattatg tagcagacta tgagaattta ataaaaaaga
     2401 tatataggat gctgaaggct ggtggcaatt tagtttttac agttgaacat cctgttttta
     2461 ctgctcatgg aacacaagac tggtattata acgaaaaagg agaaatactg catttcccgg
     2521 tggacaatta ttattatgag ggcaaacgga cagctatgtt tttggaagaa aaggttacaa
     2581 aatatcatag aacactgacc acatatctaa atacactgct ttcaaatagt tttataataa
     2641 atcagattgt ggagccacag ccgccagaga acatgatgga tattccgggg atggcggatg
     2701 aaatgcgacg cccaatgatg ctgattgtat cggcaaaaaa gaagatgtaa taatatagaa
     2761 aaaataaacg aggagtatgt aaatgagatc agaaaaagaa atgatggatt tagtactttc
     2821 tttagcagaa caggatgaac gtattcgaat tgtgaccctt gaggggtcac gcgcaaatat
     2881 taatatacct aaagatgaat ttcaggatta tgatattaca tattttgtaa gtgatataga
     2941 accgtttata tctaatgatg actggcttaa tcaatttggg aatataataa tgatgcaaaa
     3001 gccggaggat atggaattat tcccacctga agaaaaggga ttttcctatc ttatgctatt
     3061 tgatgattac aataaaattg atcttacctt attgcccttg gaagagttag ataattacct
     3121 aaagggcgat aaattaataa aggttctaat tgataaagat tgtagaatta aaagggacat
     3181 agttccgact gatatagatt atcatgtaag aaagccaagc gcaagggagt atgatgattg
     3241 ctgcaatgaa ttttggaatg taacacctta tgttattaaa ggattgtgcc gtaaggaaat
     3301 tttatttgct attgatcatt ttaatcagat tgttcgccat gagctgctga gaatgatatc
     3361 atggaaggtc ggcatcgaaa caggctttaa attaagtgta ggcaagaact ataagtttat
     3421 tgaaaggtat atatccgagg atttgtggga gaaacttttg tccacctacc ggatggattc
     3481 ctatgaaaac atatgggaag cattatttct atgccatcaa ttgttcaggg cggtatccgg
     3541 tgaggtggcg gaaaggcttc attatgccta tccggagtat gataggaata taacaaaata
     3601 taccagggac atgtataaaa aatacactgg taaaaccggc tgcctggata gcacatatgc
     3661 cgctgatata gaagagaggc gggaacagtg attacagaaa tgaaagcagg gcacctgaaa
     3721 gatatcgata aacccagcga accatttgag gtgataggta agattatacc gaggtatgaa
     3781 aacgagaatt ggacctttac agaattactc tatgaagcgc catatttaaa aagctaccaa
     3841 gacgaagagg atgaagagga tgaggaggca gattgccttg aatatattga caatactgat
     3901 aagataatat atctttacta ccaagacgat aaatgcgtcg gaaaagttaa actgcgaaaa
     3961 aattggaacc ggtacgctta tatagaagat atcgccgtat gtaaggattt cagggggcaa
     4021 ggcataggca gcgcgcttat caatatatct atagaatggg caaagcataa aaacttgcat
     4081 ggactaatgc ttgaaaccca ggacaataac cttatagctt gtaaattcta tcataattgt
     4141 ggtttcaaaa tcggctccgt cgatactatg ttatacgcca actttgaaaa caactttgaa
     4201 aaagctgttt tctggtattt aaggttttag aatgcaagga acagtgaatt ggagttcgtc
     4261 ttgttataat tagcttcttg gggtatcttt aaatactgta gaaaagagga aggaaataat
     4321 aaatggctaa aatgagaata tcaccggaat tgaaaaaact gatcgaaaaa taccgctgcg
     4381 taaaagatac ggaaggaatg tctcctgcta aggtatataa gctggtggga gaaaatgaaa
     4441 acctatattt aaaaatgacg gacagccggt ataaagggac cacctatgat gtggaacggg
     4501 aaaaggacat gatgctatgg ctggaaggaa agctgcctgt tccaaaggtc ctgcactttg
     4561 aacggcatga tggctggagc aatctgctca tgagtgaggc cgatggcgtc ctttgctcgg
     4621 aagagtatga agatgaacaa agccctgaaa agattatcga gctgtatgcg gagtgcatca
     4681 ggctctttca ctccatcgac atatcggatt gtccctatac gaatagctta gacagccgct
     4741 tagccgaatt ggattactta ctgaataacg atctggccga tgtggattgc gaaaactggg
     4801 aagaagacac tccatttaaa gatccgcgcg agctgtatga ttttttaaag acggaaaagc
     4861 ccgaagagga acttgtcttt tcccacggcg acctgggaga cagcaacatc tttgtgaaag
     4921 atggcaaagt aagtggcttt attgatcttg ggagaagcgg cagggcggac aagtggtatg
     4981 acattgcctt ctgcgtccgg tcgatcaggg aggatatcgg ggaagaacag tatgtcgagc
     5041 tattttttga cttactgggg atcaagcctg attgggagaa aataaaatat tatattttac
     5101 tggatgaatt gttttagtac ctagatttag atgtctaaaa agctttaact acaagctttt
     5161 tagacatcta atcttttctg aagtacatcc gcaactgtcc atactctgat gttttatatc
     5221 ttttctaaaa gttcgctaga tagagttcta tattagagat gtaaagagtt ttggtgaaga
     5281 ggtttgtgag tatggctttt ataaccaggg tgcaatgaga agcactaaca gcagctagct
     5341 taagaacgca taggatatac tttttatgga agtcacagag gaggaataaa atttgaacag
     5401 gattaacaga gtaacctcta ttcttattca gctgcaatca aaaaaaataa ttcctgccaa
     5461 agaaattgca cagcgattta atataagctt aaggacagtt tacagagata tccggacact
     5521 tgaagaagcc ggaataccaa ttggatctga ggccggaaaa ggatattttc ttgtagaggg
     5581 cttcctactt ccgccggtaa tgtttacagc ggcggaagtg ggtgcattga ttacagcagg
     5641 gaaattttta aattgccatg gagatgaatc atttataaag gattttgatt cagctatgta
     5701 taaaatcaaa tctattttga agcatggtga aaaaaactat gcacaggagt tggagaacag
     5761 tattaacgtg tacagcacat ctggacagaa aaatacattg gcggataacg ttattgcagc
     5821 aattcagact gcaatctgca ataaaagggt aatatcaatt caatatcctg catcaggagg
     5881 gcaagaaccg gaaagcagaa tgatagagcc tatatcactg gggttttatg aacagaactg
     5941 gtacttaatc ggttttgcag gctaagaaat gaatatagga attttcgtgt ggatcggatt
     6001 aaaagcattt gcatagaaga agagctctgt caaagccatg atggctcttt ggaacaaata
     6061 ttaaaacaaa tgctttcata taaaaaacta tataatgtaa ttcttagagc tgaaaaaggg
     6121 gagacataca attctatcaa gaacaggtat tctctcggct tccttgaaga aaccgatttg
     6181 ggaagtaaaa tggaaatcga attccagaca gattcctttg agatattgag caagcagctg
     6241 attgaatatg gatcaggcat tgaaatagtc caacctgatg aattaaaatg cataacccgt
     6301 aaacacctgg cacaaattac gaatcactgc ctaaacttga tttagagaaa gcgattggtc
     6361 tgttatgtat tttttgtaag gtttcatatc ccctgctgac atacagctgt cagtgtatta
     6421 tgatattcta ctatcaaaaa ggaggattga aaaatatgaa accaaagata attatgcaca
     6481 cacaaattag ccttgatggc cgcattaaag gctttgataa tccggaagtt tactaccagg
     6541 ttgccggggg aattcattcg gatgcggtgc tgtttgggtc caatactgtt tttactgcat
     6601 ttgaaaaata tcctgctgaa acagaggctg attttggata aagtccgtat aattgtgtaa
     6661 aagtaaaaag gccata
//
LOCUS       DAZUKC010000281         6658 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.guided.123, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000281 DAZUKC010000000
VERSION     DAZUKC010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.guided.123"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            272..493
                     /gene="relB"
                     /locus_tag="ACG80T_003009"
     CDS             272..493
                     /gene="relB"
                     /locus_tag="ACG80T_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFS4842555.1"
                     /translation="MTVISLRIDTQEDKLIKEYAKAKNISVSALIRSSVLEKIEDEID
                     LGLYNQAMKDHKENPKSISFEDMLKELSD"
     gene            494..766
                     /locus_tag="ACG80T_003010"
     CDS             494..766
                     /locus_tag="ACG80T_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS4842556.1"
                     /translation="MSSGYQVEFEKGAQKALKKMDKYQSLLIMGWIQKNLVNCTDPRQ
                     HGKGLTVNRSGEWRYRIGDYRLIADINDETVTILMLEIGHRKNIYK"
     gene            complement(897..3863)
                     /locus_tag="ACG80T_003011"
     CDS             complement(897..3863)
                     /locus_tag="ACG80T_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFS4842557.1"
                     /translation="MKIARGRELLTPEQRQAFMQIPEDEWILGTYFTFSKRDLEIVNK
                     RRREENRLGFAVQLAVLRYPGWPYTHIKSIPDSVIQYISKQIGVSPSSLDHYPQRENT
                     LWDHLKEIRSEYDFVTFTLSEYRMTFKYLHQLALENGDAIHLLHECIDFLRKNKIILP
                     AITTLERMVWEARAMAEKKLFNTVSKSLTNEQKEKLEGIITSQHPSESNKTILGWLKE
                     PPGHPSPETFLKIIERLEYIRGMDLETVQISHLHRNRLLQLSRLGSRYEPYAFRDFQE
                     NKRYSILTIYLLQLTQELTDKAFEIHDRQILSLLSKGRKAQEEIQKQNGKKLNEKVIH
                     FTNIGQALIKAREEKLDVFKVLESVIEWNTFVSSVEEAQELARPADYDYLDLLQKRFY
                     SLRKYTPTLLRVLEFHSTKANEPLLQAVEIIRGMNESGKRKVPDDSPVDFISKRWKRH
                     LYEDDGTTINRHYYEMAVLTELREHVRAGDVSIVGSRQYRDFEEYLFSEDTWNQSKGN
                     TRLSVSLSFEDYITERTSSFNERLKWLAANSNKLDGVSLEKGKLSLARLEKDVPEEAK
                     KFSASLYQMLPRIKLTDLLMDVAHITGFHEQFTHASNNRKPDKEETIIIMAALLGMGM
                     NIGLSKMAEATPGLTYKQLANVSQWRMYEDAMNKAQAILVNFHHKLQLPFYWGDGTTS
                     SSDGMRMQLGVSSLHADANPHYGTGKGATIYRFTSDQFSSYYTKIIHTNSRDAIHVLD
                     GLLHHETDLNIEEHYTDTAGYTDQIFGLTHLLGFKFAPRIRDLSDSKLFTIDKASEYP
                     KLEAILRGQINTKVIKENYEDVLRLAHSIREGTVSASLIMGKLGSYSRQNSLATALRE
                     MGRIEKTIFILNYISDESLRRKIQRGLNKGEAMNGLARAIFFGKQGELRERTIQHQLQ
                     RASALNIIINAISIWNTLHLTTAVEYKKRTGSFNEDLLHHMSPLGWEHINLLGEYHFN
                     SEKVVSLNSLRPLKLS"
     gene            4009..4584
                     /locus_tag="ACG80T_003012"
     CDS             4009..4584
                     /locus_tag="ACG80T_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4842558.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            4798..5493
                     /gene="vanR-A"
                     /locus_tag="ACG80T_003013"
     CDS             4798..5493
                     /gene="vanR-A"
                     /locus_tag="ACG80T_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFS4842559.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            5471..6640
                     /gene="vanS"
                     /locus_tag="ACG80T_003014"
     CDS             5471..6640
                     /gene="vanS"
                     /locus_tag="ACG80T_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS"
                     /protein_id="HFS4842560.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVMYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRGSVAKF"
BASE COUNT         2102 a         1209 c         1271 g         2076 t
ORIGIN      
        1 ttctttaaaa atgaatcgag ttatttggca aaatattatt ttctcaatgg tagttgtcgc
       61 ttttctaatt accgtaagct tagtaggatt aacggatata gccattagtg tgattgttca
      121 tgaaggaagt accttagtcg tgatattaaa tggtcttcga ttattaaagt ctaaataaat
      181 acatgaaatt ggataaaatc atgtatctaa agtttgcagc ctctctaatt ttgtattaca
      241 atgtatatac aaaaagaagg gggtgattct aatgactgtc atatcgctca gaattgatac
      301 tcaggaagat aaattaatta aagaatatgc taaggcgaag aatatttcgg tttcggctct
      361 gattagaagt tctgtcttgg aaaaaatcga ggatgaaatt gatttgggcc tatataatca
      421 agccatgaag gatcataaag agaatccgaa aagtatttct tttgaagaca tgctaaagga
      481 gctatctgat taaatgagct caggttatca agtcgagttt gaaaaaggag ctcaaaaagc
      541 gttaaaaaaa atggacaaat accaatccct tttaattatg ggatggattc aaaagaatct
      601 agtgaattgt accgatccta ggcagcatgg aaaaggatta accgtcaatc gatcaggcga
      661 atggcgctat agaatcggtg attatcgctt aatagctgat atcaatgacg agaccgtaac
      721 cattttaatg ttagagattg gtcatagaaa aaatatttac aaataaacca ttcaaattga
      781 gtgcaagcat gtttaataaa aaaggattct actgcttttt aaggggtagc gtcaggaaaa
      841 tgcggattta caacgctaag cctattttcc tgacgaatcc ctcgttttta acaacgttaa
      901 gaaagtttta gtggtcttaa agaatttaat gagactactt tctctgagtt aaaatggtat
      961 tctcctagta aattaatatg ttcccaacct aagggcgaca tatggtgtaa caaatcttca
     1021 ttaaagctac ctgtccgttt tttatattca actgctgttg ttaggtggag agtattccaa
     1081 atacttatag cattgataat tatgtttaaa gcactggctc tttgcaattg atgctgtatg
     1141 gtgcgttctc taagctcacc ttgttttccg aagaaaatag ctcttgccaa tccattcatg
     1201 gcttctcctt tattcaatcc tctttgtatt tttcttctta atgattcatc cgatatataa
     1261 ttcaaaataa agatcgtttt ttctattcgg cccatctcac gtaaggctgt agctaagctg
     1321 ttttgtcttg aataggaacc tagcttcccc ataataaggg atgctgaaac tgttccctcc
     1381 cttatagaat gagctaatcg caaaacatcc tcataatttt ctttaatgac ctttgtattt
     1441 atttgtccac gtaaaatggc ttctagtttt ggatactcac ttgctttatc tatcgtaaat
     1501 aattttgagt ccgataaatc ccttattctt ggggcaaatt taaatcctaa taaatgagtc
     1561 agtccgaata tttggtcagt gtaaccggca gtgtctgtat aatgttcctc tatgtttaga
     1621 tccgtctcat gatgtaacaa accatccaaa acatgaatcg catctcttga attagtatga
     1681 ataatctttg tgtagtaaga agagaattga tcacttgtaa atcggtagat ggtggctcct
     1741 tttccagttc cataatgtgg atttgcatct gcatgtagtg atgaaacacc tagctgcatt
     1801 ctcataccat ctgacgaaga tgttgtaccg tcgccccaat agaaaggcaa ttgtaattta
     1861 tgatgaaagt ttactaatat ggcttgggct ttattcatgg catcttcata catgcgccat
     1921 tgagatacat tggctagttg cttatatgta agtccgggtg tggcttcggc catcttgctc
     1981 aagccaatat tcattcccat tcctaaaagg gcagccatga taatgattgt ttcttcctta
     2041 tctggttttc gattattgga agcatgagtg aattgctcat gaaatcctgt tatatgggcc
     2101 acatccatga gtaaatcagt taattttatt cttggtagca tctgataaag gcttgcacta
     2161 aatttttttg cttcttctgg aacatctttt tctaagcgtg caagtgatag ctttcctttt
     2221 tcaagagaaa ccccatctaa cttattggaa ttggcagcta accactttaa cctttcatta
     2281 aagctgctgg ttctctccgt tatataatct tcgaatgata aactaactga taatctcgta
     2341 ttccccttcg attgattcca tgtatcttcc gaaaacaaat attcctcaaa atccctatat
     2401 tgtctgctgc caacaatgga aacatctcct gcccgaacat gctcccgaag ttctgttaaa
     2461 acagccattt catagtaatg acgattaatt gttgtaccat catcctcgta taaatgtctt
     2521 ttccatcgtt ttgaaataaa atccacaggt gagtcatcag gcacttttcg ctttccagat
     2581 tcgttcattc ctcggataat ctcaacagct tgtaaaagtg gctcatttgc ctttgtagaa
     2641 tgaaattcca atactcttaa tagcgttggc gtatattttc ttagtgaata aaaccgtttt
     2701 tgcagtaagt ctaaataatc atagtcggca ggacgtgcaa gttcctgagc ctcttctact
     2761 gaagagacaa aggtattcca ttcaataacc gattctaaaa ccttaaaaac gtctaatttt
     2821 tcctctcttg ctttaattaa tgcttgtccg atgttcgtaa agtgtataac tttctcattt
     2881 agctttttac cgttttgttt ctggatttcc tcttgagcct tacgaccttt tgataacaaa
     2941 ctaagtattt gcctatcatg aatttcaaac gctttatccg ttagctcctg agtaagttgt
     3001 aataaataga tggttaatat cgaataacgt ttattttctt gaaagtcacg gaatgcatac
     3061 ggctcgtatc ttgagcctaa gcgagacagc tgcaacaggc ggttacggtg caaatgacta
     3121 atttgcactg tttctaaatc cattcctcgt atgtattcga gtcgttctat tatttttaga
     3181 aaagtttcgg gtgaaggatg acccggtggc tcttttaacc aacccaatat cgttttattg
     3241 gattcggatg gatgctgcga ggtaataatc ccttcaagct tttctttttg ctcatttgtt
     3301 agagatttac taaccgtatt aaatagcttc ttttcagcca ttgcccttgc ttcccacacc
     3361 attctttcaa gtgtagtgat agcaggcagt ataattttgt tttttcttag aaaatctatg
     3421 cattcatgca gtagatgaat ggcatcacca ttttccaaag ctaattgatg aaggtactta
     3481 aatgtcattc gatattcact cagggtaaaa gttacaaagt cgtattcact tcgaatttct
     3541 ttcaaatgat cccaaagtgt attttccctt tgaggataat gatcaagcga ggatggacta
     3601 acaccaatct gtttcgatat atattgtatg accgaatctg ggatgctttt gatatgagtg
     3661 tatggccaac cgggataccg aagaacagct aattgaacag caaatcctaa acggttttct
     3721 tccctccttc gcttattaac tatttctaaa tcccgtttgg aaaaagtgaa gtaggtcccc
     3781 agtatccatt catcttcagg gatttgcata aaagcctgtc tctgttccgg tgtaagcaat
     3841 tctctacctc tcgcaatttt cattcagtat cattccattt ctgtattttc aatttattag
     3901 ttcaattata tatcaataga gtgtactcta ttgatacaaa tgtagtagac tgataaaatc
     3961 atagttaaga gcgtctcata agacttgtct caaaaatgag gtgatatttt gcggaaaatc
     4021 ggttatattc gtgtcagttc gactaaccag aatccttcaa gacaatttca gcagttgaac
     4081 gagatcggaa tggatattat atatgaagag aaagtttcag gagcaacaaa ggatcgcgag
     4141 caacttcaaa aagtgttaga cgatttacag gaagatgaca tcatttatgt tacagactta
     4201 actcgaatca ctcgtagtac acaagatcta tttgaattaa tcgataacat acgagataaa
     4261 aaggcaagtt taaaatcact aaaagataca tggcttgatt tatcagaaga taatccatac
     4321 agccaattct taattactgt aatggctggt gttaaccaat tagagcgaga tcttattcgg
     4381 atgagacaac gtgaagggat tgaattggct aagaaagaag gaaagtttaa aggtcgatta
     4441 aagaagtatc ataaaaatca cgcaggaatg aattatgcgg taaagctata taaagaagga
     4501 aatatgactg taaatcaaat ttgtgaaatt actaatgtat ctagggcttc attatacagg
     4561 aaattatcag aagtgaataa ttagccattc tgtattccgc taatgggcaa tatttttaaa
     4621 gaagaaaagg aaactataaa atattaacag cctcctagcg atgccgaaaa gccctttgat
     4681 aaaaaaagaa tcatcatctt aagaaattct tagtcattta ttatgtaaat gcttataaat
     4741 tcggccctat aatctgataa attattaagg gcaaacttat gtgaaagggt gataactatg
     4801 agcgataaaa tacttattgt ggatgatgaa catgaaattg ccgatttggt tgaattatac
     4861 ttaaaaaacg agaattatac ggttttcaaa tactataccg ccaaagaagc attggaatgt
     4921 atagacaagt ctgagattga ccttgccata ttggacatca tgcttcccgg cacaagcggc
     4981 cttactatct gtcaaaaaat aagggacaag cacacctatc cgattatcat gctgaccggg
     5041 aaagatacag aggtagataa aattacaggg ttaacaatcg gcgcggatga ttatataacg
     5101 aagccctttc gcccactgga gttaattgct cgggtaaagg cccagttgcg ccgatacaaa
     5161 aaattcagtg gagtaaagga gcagaacgaa aatgttatcg tccactccgg ccttgtcatt
     5221 aatgttaaca cccatgagtg ttatctgaac gagaagcagt tatcccttac tcccaccgag
     5281 ttttcaatac tgcgaatcct ctgtgaaaac aaggggaatg tggttagctc cgagctgcta
     5341 tttcatgaga tatggggcga cgaatatttc agcaagagca acaacaccat caccgtgcat
     5401 atccggcatt tgcgcgaaaa aatgaacgac accattgata atccgaaata tataaaaacg
     5461 gtatgggggg ttggttataa aattgaaaaa taaaaaaaac gactattcca aactagaacg
     5521 aaaactttac atgtatatcg ttgcaattgt tgtggtagca attgtattcg tgatgtatat
     5581 tcgttcaatg atccgaggga aacttgggga ttggatctta agtattttgg aaaacaaata
     5641 tgacttaaat cacctggacg cgatgaaatt atatcaatat tccatacgga acaatataga
     5701 tatctttatt tatgtggcga ttgtcattag tattcttatt ctatgtcgcg tcatgctttc
     5761 aaaattcgca aaatactttg acgagataaa taccggcatt gatgtactta ttcagaacga
     5821 agataaacaa attgagcttt ctgcggaaat ggatgttatg gaacaaaagc tcaacacatt
     5881 aaaacggact ctggaaaagc gagagcagga tgcaaagctg gccgaacaaa gaaaaaatga
     5941 cgttgttatg tacttggcgc acgatattaa aacgcccctt acatccatta tcggttattt
     6001 gagcctgctt gacgaggctc cagacatgcc ggtagatcaa aaggcaaagt atgtgcatat
     6061 cacgttggac aaagcgtatc gactcgaaca gctaatcgac gagttttttg agattacacg
     6121 gtataaccta caaacgataa cgctaacaaa aacgcacata gacctatact atatgctggt
     6181 gcagatgacc gatgaatttt atcctcagct ttccgcacat ggaaaacagg cggttattca
     6241 cgcccccgag gatctgaccg tgtccggcga ccctgataaa ctcgcgagag tctttaacaa
     6301 cattttgaaa aacgccgctg catacagtga ggataacagc atcattgaca ttaccgcggg
     6361 cctctccggg gatgtggtgt caatcgaatt caagaacact ggaagcatcc caaaagataa
     6421 gctagctgcc atatttgaaa agttctatag gctggacaat gctcgttctt ccgatacggg
     6481 tggcgcggga cttggattgg cgattgcaaa agaaattatt gttcagcatg gagggcagat
     6541 ttacgcggaa agcaatgata actatacgac gtttagggta gagcttccag cgatgccaga
     6601 cttggttgat aaaaggaggg gttctgttgc aaagttttaa ataaagaata aaatccct
//
LOCUS       DAZUKC010000282         6658 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.guided.124, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000282 DAZUKC010000000
VERSION     DAZUKC010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.guided.124"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            272..493
                     /gene="relB"
                     /locus_tag="ACG80T_003015"
     CDS             272..493
                     /gene="relB"
                     /locus_tag="ACG80T_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFS4842561.1"
                     /translation="MTVISLRIDTQEDKLIKEYAKAKNISVSALIRSSVLEKIEDEID
                     LGLYNQAMKDHKENPKSISFEDMLKELSD"
     gene            494..766
                     /locus_tag="ACG80T_003016"
     CDS             494..766
                     /locus_tag="ACG80T_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS4842562.1"
                     /translation="MSSGYQVEFEKGAQKALKKMDKYQSLLIMGWIQKNLVNCTDPRQ
                     HGKGLTVNRSGEWRYRIGDYRLIADINDETVTILMLEIGHRKNIYK"
     gene            complement(897..3863)
                     /locus_tag="ACG80T_003017"
     CDS             complement(897..3863)
                     /locus_tag="ACG80T_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFS4842563.1"
                     /translation="MKIARGRELLTPEQRQAFMQIPEDEWILGTYFTFSKRDLEIVNK
                     RRREENRLGFAVQLAVLRYPGWPYTHIKSIPDSVIQYISKQIGVSPSSLDHYPQRENT
                     LWDHLKEIRSEYDFVTFTLSEYRMTFKYLHQLALENGDAIHLLHECIDFLRKNKIILP
                     AITTLERMVWEARAMAEKKLFNTVSKSLTNEQKEKLEGIITSQHPSESNKTILGWLKE
                     PPGHPSPETFLKIIERLEYIRGMDLETVQISHLHRNRLLQLSRLGSRYEPYAFRDFQE
                     NKRYSILTIYLLQLTQELTDKAFEIHDRQILSLLSKGRKAQEEIQKQNGKKLNEKVIH
                     FTNIGQALIKAREEKLDVFKVLESVIEWNTFVSSVEEAQELARPADYDYLDLLQKRFY
                     SLRKYTPTLLRVLEFHSTKANEPLLQAVEIIRGMNESGKRKVPDDSPVDFISKRWKRH
                     LYEDDGTTINRHYYEMAVLTELREHVRAGDVSIVGSRQYRDFEEYLFSEDTWNQSKGN
                     TRLSVSLSFEDYITERTSSFNERLKWLAANSNKLDGVSLEKGKLSLARLEKDVPEEAK
                     KFSASLYQMLPRIKLTDLLMDVAHITGFHEQFTHASNNRKPDKEETIIIMAALLGMGM
                     NIGLSKMAEATPGLTYKQLANVSQWRMYEDAMNKAQAILVNFHHKLQLPFYWGDGTTS
                     SSDGMRMQLGVSSLHADANPHYGTGKGATIYRFTSDQFSSYYTKIIHTNSRDAIHVLD
                     GLLHHETDLNIEEHYTDTAGYTDQIFGLTHLLGFKFAPRIRDLSDSKLFTIDKASEYP
                     KLEAILRGQINTKVIKENYEDVLRLAHSIREGTVSASLIMGKLGSYSRQNSLATALRE
                     MGRIEKTIFILNYISDESLRRKIQRGLNKGEAMNGLARAIFFGKQGELRERTIQHQLQ
                     RASALNIIINAISIWNTLHLTTAVEYKKRTGSFNEDLLHHMSPLGWEHINLLGEYHFN
                     SEKVVSLNSLRPLKLS"
     gene            4009..4584
                     /locus_tag="ACG80T_003018"
     CDS             4009..4584
                     /locus_tag="ACG80T_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4842564.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            4798..5493
                     /gene="vanR-A"
                     /locus_tag="ACG80T_003019"
     CDS             4798..5493
                     /gene="vanR-A"
                     /locus_tag="ACG80T_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFS4842565.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            5471..6640
                     /gene="vanS"
                     /locus_tag="ACG80T_003020"
     CDS             5471..6640
                     /gene="vanS"
                     /locus_tag="ACG80T_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS"
                     /protein_id="HFS4842566.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRGSVAKF"
BASE COUNT         2101 a         1209 c         1271 g         2077 t
ORIGIN      
        1 ttctttaaaa atgaatcgag ttatttggca aaatattatt ttctcaatgg tagttgtcgc
       61 ttttctaatt accgtaagct tagtaggatt aacggatata gccattagtg tgattgttca
      121 tgaaggaagt accttagtcg tgatattaaa tggtcttcga ttattaaagt ctaaataaat
      181 acatgaaatt ggataaaatc atgtatctaa agtttgcagc ctctctaatt ttgtattaca
      241 atgtatatac aaaaagaagg gggtgattct aatgactgtc atatcgctca gaattgatac
      301 tcaggaagat aaattaatta aagaatatgc taaggcgaag aatatttcgg tttcggctct
      361 gattagaagt tctgtcttgg aaaaaatcga ggatgaaatt gatttgggcc tatataatca
      421 agccatgaag gatcataaag agaatccgaa aagtatttct tttgaagaca tgctaaagga
      481 gctatctgat taaatgagct caggttatca agtcgagttt gaaaaaggag ctcaaaaagc
      541 gttaaaaaaa atggacaaat accaatccct tttaattatg ggatggattc aaaagaatct
      601 agtgaattgt accgatccta ggcagcatgg aaaaggatta accgtcaatc gatcaggcga
      661 atggcgctat agaatcggtg attatcgctt aatagctgat atcaatgacg agaccgtaac
      721 cattttaatg ttagagattg gtcatagaaa aaatatttac aaataaacca ttcaaattga
      781 gtgcaagcat gtttaataaa aaaggattct actgcttttt aaggggtagc gtcaggaaaa
      841 tgcggattta caacgctaag cctattttcc tgacgaatcc ctcgttttta acaacgttaa
      901 gaaagtttta gtggtcttaa agaatttaat gagactactt tctctgagtt aaaatggtat
      961 tctcctagta aattaatatg ttcccaacct aagggcgaca tatggtgtaa caaatcttca
     1021 ttaaagctac ctgtccgttt tttatattca actgctgttg ttaggtggag agtattccaa
     1081 atacttatag cattgataat tatgtttaaa gcactggctc tttgcaattg atgctgtatg
     1141 gtgcgttctc taagctcacc ttgttttccg aagaaaatag ctcttgccaa tccattcatg
     1201 gcttctcctt tattcaatcc tctttgtatt tttcttctta atgattcatc cgatatataa
     1261 ttcaaaataa agatcgtttt ttctattcgg cccatctcac gtaaggctgt agctaagctg
     1321 ttttgtcttg aataggaacc tagcttcccc ataataaggg atgctgaaac tgttccctcc
     1381 cttatagaat gagctaatcg caaaacatcc tcataatttt ctttaatgac ctttgtattt
     1441 atttgtccac gtaaaatggc ttctagtttt ggatactcac ttgctttatc tatcgtaaat
     1501 aattttgagt ccgataaatc ccttattctt ggggcaaatt taaatcctaa taaatgagtc
     1561 agtccgaata tttggtcagt gtaaccggca gtgtctgtat aatgttcctc tatgtttaga
     1621 tccgtctcat gatgtaacaa accatccaaa acatgaatcg catctcttga attagtatga
     1681 ataatctttg tgtagtaaga agagaattga tcacttgtaa atcggtagat ggtggctcct
     1741 tttccagttc cataatgtgg atttgcatct gcatgtagtg atgaaacacc tagctgcatt
     1801 ctcataccat ctgacgaaga tgttgtaccg tcgccccaat agaaaggcaa ttgtaattta
     1861 tgatgaaagt ttactaatat ggcttgggct ttattcatgg catcttcata catgcgccat
     1921 tgagatacat tggctagttg cttatatgta agtccgggtg tggcttcggc catcttgctc
     1981 aagccaatat tcattcccat tcctaaaagg gcagccatga taatgattgt ttcttcctta
     2041 tctggttttc gattattgga agcatgagtg aattgctcat gaaatcctgt tatatgggcc
     2101 acatccatga gtaaatcagt taattttatt cttggtagca tctgataaag gcttgcacta
     2161 aatttttttg cttcttctgg aacatctttt tctaagcgtg caagtgatag ctttcctttt
     2221 tcaagagaaa ccccatctaa cttattggaa ttggcagcta accactttaa cctttcatta
     2281 aagctgctgg ttctctccgt tatataatct tcgaatgata aactaactga taatctcgta
     2341 ttccccttcg attgattcca tgtatcttcc gaaaacaaat attcctcaaa atccctatat
     2401 tgtctgctgc caacaatgga aacatctcct gcccgaacat gctcccgaag ttctgttaaa
     2461 acagccattt catagtaatg acgattaatt gttgtaccat catcctcgta taaatgtctt
     2521 ttccatcgtt ttgaaataaa atccacaggt gagtcatcag gcacttttcg ctttccagat
     2581 tcgttcattc ctcggataat ctcaacagct tgtaaaagtg gctcatttgc ctttgtagaa
     2641 tgaaattcca atactcttaa tagcgttggc gtatattttc ttagtgaata aaaccgtttt
     2701 tgcagtaagt ctaaataatc atagtcggca ggacgtgcaa gttcctgagc ctcttctact
     2761 gaagagacaa aggtattcca ttcaataacc gattctaaaa ccttaaaaac gtctaatttt
     2821 tcctctcttg ctttaattaa tgcttgtccg atgttcgtaa agtgtataac tttctcattt
     2881 agctttttac cgttttgttt ctggatttcc tcttgagcct tacgaccttt tgataacaaa
     2941 ctaagtattt gcctatcatg aatttcaaac gctttatccg ttagctcctg agtaagttgt
     3001 aataaataga tggttaatat cgaataacgt ttattttctt gaaagtcacg gaatgcatac
     3061 ggctcgtatc ttgagcctaa gcgagacagc tgcaacaggc ggttacggtg caaatgacta
     3121 atttgcactg tttctaaatc cattcctcgt atgtattcga gtcgttctat tatttttaga
     3181 aaagtttcgg gtgaaggatg acccggtggc tcttttaacc aacccaatat cgttttattg
     3241 gattcggatg gatgctgcga ggtaataatc ccttcaagct tttctttttg ctcatttgtt
     3301 agagatttac taaccgtatt aaatagcttc ttttcagcca ttgcccttgc ttcccacacc
     3361 attctttcaa gtgtagtgat agcaggcagt ataattttgt tttttcttag aaaatctatg
     3421 cattcatgca gtagatgaat ggcatcacca ttttccaaag ctaattgatg aaggtactta
     3481 aatgtcattc gatattcact cagggtaaaa gttacaaagt cgtattcact tcgaatttct
     3541 ttcaaatgat cccaaagtgt attttccctt tgaggataat gatcaagcga ggatggacta
     3601 acaccaatct gtttcgatat atattgtatg accgaatctg ggatgctttt gatatgagtg
     3661 tatggccaac cgggataccg aagaacagct aattgaacag caaatcctaa acggttttct
     3721 tccctccttc gcttattaac tatttctaaa tcccgtttgg aaaaagtgaa gtaggtcccc
     3781 agtatccatt catcttcagg gatttgcata aaagcctgtc tctgttccgg tgtaagcaat
     3841 tctctacctc tcgcaatttt cattcagtat cattccattt ctgtattttc aatttattag
     3901 ttcaattata tatcaataga gtgtactcta ttgatacaaa tgtagtagac tgataaaatc
     3961 atagttaaga gcgtctcata agacttgtct caaaaatgag gtgatatttt gcggaaaatc
     4021 ggttatattc gtgtcagttc gactaaccag aatccttcaa gacaatttca gcagttgaac
     4081 gagatcggaa tggatattat atatgaagag aaagtttcag gagcaacaaa ggatcgcgag
     4141 caacttcaaa aagtgttaga cgatttacag gaagatgaca tcatttatgt tacagactta
     4201 actcgaatca ctcgtagtac acaagatcta tttgaattaa tcgataacat acgagataaa
     4261 aaggcaagtt taaaatcact aaaagataca tggcttgatt tatcagaaga taatccatac
     4321 agccaattct taattactgt aatggctggt gttaaccaat tagagcgaga tcttattcgg
     4381 atgagacaac gtgaagggat tgaattggct aagaaagaag gaaagtttaa aggtcgatta
     4441 aagaagtatc ataaaaatca cgcaggaatg aattatgcgg taaagctata taaagaagga
     4501 aatatgactg taaatcaaat ttgtgaaatt actaatgtat ctagggcttc attatacagg
     4561 aaattatcag aagtgaataa ttagccattc tgtattccgc taatgggcaa tatttttaaa
     4621 gaagaaaagg aaactataaa atattaacag cctcctagcg atgccgaaaa gccctttgat
     4681 aaaaaaagaa tcatcatctt aagaaattct tagtcattta ttatgtaaat gcttataaat
     4741 tcggccctat aatctgataa attattaagg gcaaacttat gtgaaagggt gataactatg
     4801 agcgataaaa tacttattgt ggatgatgaa catgaaattg ccgatttggt tgaattatac
     4861 ttaaaaaacg agaattatac ggttttcaaa tactataccg ccaaagaagc attggaatgt
     4921 atagacaagt ctgagattga ccttgccata ttggacatca tgcttcccgg cacaagcggc
     4981 cttactatct gtcaaaaaat aagggacaag cacacctatc cgattatcat gctgaccggg
     5041 aaagatacag aggtagataa aattacaggg ttaacaatcg gcgcggatga ttatataacg
     5101 aagccctttc gcccactgga gttaattgct cgggtaaagg cccagttgcg ccgatacaaa
     5161 aaattcagtg gagtaaagga gcagaacgaa aatgttatcg tccactccgg ccttgtcatt
     5221 aatgttaaca cccatgagtg ttatctgaac gagaagcagt tatcccttac tcccaccgag
     5281 ttttcaatac tgcgaatcct ctgtgaaaac aaggggaatg tggttagctc cgagctgcta
     5341 tttcatgaga tatggggcga cgaatatttc agcaagagca acaacaccat caccgtgcat
     5401 atccggcatt tgcgcgaaaa aatgaacgac accattgata atccgaaata tataaaaacg
     5461 gtatgggggg ttggttataa aattgaaaaa taaaaaaaac gactattcca aactagaacg
     5521 aaaactttac atgtatatcg ttgcaattgt tgtggtagca attgtattcg tgttgtatat
     5581 tcgttcaatg atccgaggga aacttgggga ttggatctta agtattttgg aaaacaaata
     5641 tgacttaaat cacctggacg cgatgaaatt atatcaatat tccatacgga acaatataga
     5701 tatctttatt tatgtggcga ttgtcattag tattcttatt ctatgtcgcg tcatgctttc
     5761 aaaattcgca aaatactttg acgagataaa taccggcatt gatgtactta ttcagaacga
     5821 agataaacaa attgagcttt ctgcggaaat ggatgttatg gaacaaaagc tcaacacatt
     5881 aaaacggact ctggaaaagc gagagcagga tgcaaagctg gccgaacaaa gaaaaaatga
     5941 cgttgttatg tacttggcgc acgatattaa aacgcccctt acatccatta tcggttattt
     6001 gagcctgctt gacgaggctc cagacatgcc ggtagatcaa aaggcaaagt atgtgcatat
     6061 cacgttggac aaagcgtatc gactcgaaca gctaatcgac gagttttttg agattacacg
     6121 gtataaccta caaacgataa cgctaacaaa aacgcacata gacctatact atatgctggt
     6181 gcagatgacc gatgaatttt atcctcagct ttccgcacat ggaaaacagg cggttattca
     6241 cgcccccgag gatctgaccg tgtccggcga ccctgataaa ctcgcgagag tctttaacaa
     6301 cattttgaaa aacgccgctg catacagtga ggataacagc atcattgaca ttaccgcggg
     6361 cctctccggg gatgtggtgt caatcgaatt caagaacact ggaagcatcc caaaagataa
     6421 gctagctgcc atatttgaaa agttctatag gctggacaat gctcgttctt ccgatacggg
     6481 tggcgcggga cttggattgg cgattgcaaa agaaattatt gttcagcatg gagggcagat
     6541 ttacgcggaa agcaatgata actatacgac gtttagggta gagcttccag cgatgccaga
     6601 cttggttgat aaaaggaggg gttctgttgc aaagttttaa ataaagaata aaatccct
//
LOCUS       DAZUKC010000283         5673 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.guided.136, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000283 DAZUKC010000000
VERSION     DAZUKC010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.guided.136"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            192..656
                     /locus_tag="ACG80T_003021"
     CDS             192..656
                     /locus_tag="ACG80T_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS4842567.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            640..1269
                     /locus_tag="ACG80T_003022"
     CDS             640..1269
                     /locus_tag="ACG80T_003022"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFS4842568.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            1623..3101
                     /gene="msr(C)"
                     /locus_tag="ACG80T_003023"
     CDS             1623..3101
                     /gene="msr(C)"
                     /locus_tag="ACG80T_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFS4842569.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            3148..3594
                     /locus_tag="ACG80T_003024"
     CDS             3148..3594
                     /locus_tag="ACG80T_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFS4842570.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(3793..4308)
                     /locus_tag="ACG80T_003025"
     CDS             complement(3793..4308)
                     /locus_tag="ACG80T_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS4842571.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            4425..5627
                     /locus_tag="ACG80T_003026"
     CDS             4425..5627
                     /locus_tag="ACG80T_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4842572.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1756 a          947 c         1205 g         1765 t
ORIGIN      
        1 taatcttggt tttagcgttg tgctttcttt atcatttttt ccgttcattc tacaaaaaat
       61 ttcttttctc tatacttttc tattgagagc gtttttttta ttacacagct gattctttga
      121 cttttttctt tttttattct attattgaca agtcaatcgt tgacgagtca attattagaa
      181 aggaggaaat tatgtcttta gaagagatta gtcacctttt atatcaaatc aaattagccg
      241 atcaaaaaat cgtccagatc tttgaagaaa atatcggtat aagtcttact agatacgaga
      301 tcatgatgag actgaaagaa gaaaatccat tgctacaaag cagtttgcaa gaaaagttgc
      361 agattgatca aggagcagtt actcgtcatc tgaaggtatt ggaagcaaat ggctatgttt
      421 cgcgtcagag aaatcctcag aacaaccggg aagtgtatgt agcgctaaca gaaaaagccc
      481 agaagaaaat caagcattgt gaaatggacc ataaagatac aggttccgtg ctatccccgg
      541 ctttcagcga tgtagaaatg aaacaatttc taacactttt agataaattg aatacccaac
      601 tttgcaatat aacaacagaa acgaaagagg ttaataataa tggaacaaaa aaatgatttt
      661 tcaacaatta tgactggtag aagatccgtt cgcgtatatg atgaaacata caaaatccca
      721 cacgaagaaa tgctggaaat gatcaaagaa gcgacgactg caccttcttc tgtcaatatg
      781 aaaccatggc gctttgtggt cgctgaaagc gaggaagcaa aagaaatctt gcgtcctttg
      841 atccgtttca atacgagaca aaatgatact tcttctgcaa tggtcatgat ttttggcgat
      901 atgcaatgtt acgaatatgg cgaagaaatc tacaaccaag cttacgaatc agggaagatg
      961 ccaaaagaag tcagagatca acaattagct gcgatcattc cttattacaa gagtttttca
     1021 agagaagaaa tgaacgatgt agtcaaagtc gattctagct tagcggctat gcaattcatg
     1081 ttagttgcgc gtgatcatgg ctatgaaaca aatccaattg gtggattcga agcagatcag
     1141 ttggcagagg catttggttt ggataaagac cgttatgttc cagttattat tttatcaata
     1201 ggaaaagcgg tagaagaagg ttacgagtct gttcgtttag caccagaaaa gatcactatg
     1261 ttcgaataaa aggttttcag ttactattca gtagctattg ctttttttga atcgtgtgtg
     1321 gtaacctaat aaaaaaggag gtcggaaaaa tgactgcatc gatgaaatta cgtttcgaac
     1381 ttttgaataa caactaaata aattcaaaag acatgtataa acaacaattt catggatgga
     1441 gtatgtccat tttttcgaac gacagtagaa taggtgaaag ctatgacaga tttggtgatt
     1501 tagtcatagt tatttcgttc agagtaggaa aagatgtgat gcagtttttt tgcaaacaga
     1561 tttttctgct cttttttagt atcgataatt gttgttttac atgaaatagg agtgtaaaac
     1621 atatggaaaa tttagcagta aatataacaa atctacaagt cagctttggc aaccagctag
     1681 aactatctat tgattctctt cgtgtctatc agcaagatcg tatagggatc ataggagaaa
     1741 atggtgtcgg taaatcgact ttgctcaaac taatagccgg tgaacttttt cccgatcatg
     1801 ggaaaatcca aacagagatc accttcaact acctgcctca attaacctat ctcgctgaag
     1861 caaaggacct aaatttggaa ttagccagtc atttccagtt aagactggaa gaaacttcag
     1921 agcggaaatg gagcggaggg gaagaacgaa agatcgagtt gatacgtctt ctttcttctt
     1981 atgaacaagg gatgcttcta gacgagccaa caacccatct agatagaaaa agtattgatc
     2041 gactgattga agagcttcgt tattattatg gtacgctggt ttttgttagt catgatcgct
     2101 attttctaga tgagctagca tcgaaaatct gggaagtaaa agacggagaa atccgagagt
     2161 tttcggggaa ttatagtgcc tatctcactc aaaaggaatt ggagaaaaag actcagctac
     2221 gagaagcaga gtcgatcatg aaagagaaaa aacgattgga aaaatcgatc caagaaaaga
     2281 aaaaacaagc ggaaaagttg gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac
     2341 tggatcggtt gtcttcctct aaacaaaaag acagtgtaca aaaagccatc caaaagaatg
     2401 cgaaaacatt agagagaaga ctccaaaaaa taggagaaac aaccaagccg cagcagatga
     2461 aacaaatccg ttttccagta ccaaaatctc ttgaactcca cagccgctat cccatcatgg
     2521 gacaaaatgt ccaattggaa agaagtggga gaacattact ggtaaatggt gattttcagt
     2581 tttctttagg taaaaaaatc gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat
     2641 tagaacatat ccgcaaacaa ggagaaggaa tccttctctc tccgaaagta agctttcaag
     2701 tatatcagca aaagggttat caaatgacat ctgaagaatc catcattcgt tttgtcatga
     2761 gacaaacaga gttttcagaa tcgcttgtcc gtagtttgct gaatcactta gggtttgctc
     2821 aggaaacttt gacgaaaccg ttatgtacat taagtggggg agaagcgacc cgtctgacga
     2881 ttgctttgct ttttactaag ccaagtaatg tgttgctgtt agatgaaccg actaatttta
     2941 ttgatatggc aacgatcgaa gctttagaga agctaatgca aatatatccg ggaacgattt
     3001 tgtttacttc tcatgattct tactttgtcg agcgtacggc tgatgaagtt tatgaaataa
     3061 aagggcagaa aataaaaaaa gtacttacga gaaattttta aatgtgctac gatgattttc
     3121 gtgacaaatt taaaggagtg gtacagattg cctacaaata aaaaagaagg aataattttt
     3181 acaacaatga tgtgtttttt gatggtcttg gggatgagcg cgtataattt atggctgcat
     3241 caagatcttc atttatcaag tttgttgatt ggtctagtgc ctggattcgt ggtggctttc
     3301 attttggatg tcttcatcat cggtgtgctg gcgaaaaaaa ttgcctttaa actaccagta
     3361 aatccagaaa gcaaggtcca aatgatctta actatttcat gcttgatggt tttaggaatg
     3421 gtgacatgta tgtctttatt cggtgtgatc atggaacaag gaatacctga tcaattattc
     3481 tctgcttata tgaatgcttg gaaaatgaat gtgattgccg ctttaccgtt gcagttgatc
     3541 atcgttggcc cgctttcaag aagtgtgcta aaagcaattc aaagtaatga atgatggaac
     3601 actgttattt gaaaaaaggc cttggcatgc tttttgtcat agtctcttga atgcagttct
     3661 agaaaaatag tgatccgaac gataggtttt ggcctgtcgt tcggatcgct ttttcttttc
     3721 caactgtatt gctcttttat tgaagataat ttgctcttca atagatattt ctagaaaggt
     3781 tctgtttatc ccttactcaa aaaactgctg catttgttct cttacttgct gacaccattg
     3841 gagttcctgc tgacaaagca taattctgcg ctggatcaca acataacgtc caaattcttg
     3901 tttcttgtct agcgagaggt cattcaattt tttttgattt ttttgcatga ttttgttcag
     3961 ctgttgttct ctagtgaata gcagtccagt agctgttgtt ttatcgagcg tcgaaaaagc
     4021 atagattttt gctagaaatt catctttttg cgtaggagcg ggagtttcct ctttgatcca
     4081 ttcctctaca agagcacgtc cttcatcagt caagtggtag atcttttttt gagagtgctc
     4141 gtctttgtcc aaaacggtca cataaccctg ctggtctaac ttggcaagcg ttgtatagat
     4201 ctgactgtga tgggcttccc aaaaaattgt gatcagctgc ttcaattcat acccagaacg
     4261 tggtttggat aaaagcacgc ataataatat ataggctaaa gtattcataa aaatcctacc
     4321 tctcgtctta ttttctttat tatatcacag taaagtttcc acgttttaca tgtgcatatt
     4381 gacatgtaaa ataaatcatg tatgatgcta atagagagaa agggatggaa atattggaga
     4441 atcaaataga tatacaagaa caaacaaatg aagaaacaaa ggggaaaagt tcagcgctag
     4501 ttgtagcagc tacctgtatg atcgctttta tgggagtggg tctagtagac ccaatcttaa
     4561 aaacgattgc tgcacagtta aacgctacgc cagctgaaac gaccctgtta tttactagtt
     4621 acatgttagt cactggtgtg gtgatgctat ttacaggttt cttatctagt cggatcggtt
     4681 tgaaaaaaac attgatgaca ggattgttta tcatcgttgt attcgctggt cttggcggat
     4741 tatctgggaa tatcggaatg ctgattgggt tacgtgccgg ctggggtgtc gggaatgctt
     4801 tatttgtctc tactgcttta gcaacgatcg tcagtatttt agttggtaac acagaaaaag
     4861 caatcatgat gtatgaagca gcacttggct gcggaatggc tgttggccct ctagtcggtg
     4921 gggtcttggg cagtggttca tggcgttatc cattttttgg ggtagcattt ttgatgtttt
     4981 gcggttttat agccctagcg gtacttttac ctgaaactga gaaaccaaag agaaaagtag
     5041 gcctttggga aggtgtcaaa gcgttaagca atcataaact tcgttcagtt ggactaatcg
     5101 cattgttata taatttcgga ttctttactt tacttgcgta tgcgccattt ttattgattg
     5161 gctattctga attagaagtc ggttttgtat tttttggctg gggtgtgctt ctggctattg
     5221 cttcgatttt cgttgcaccg gctcttgagc ggaagtttac gacttatcgg acaattttag
     5281 cagcatttgt cttattcatt atttgtttgc tgttattagg agctggaact tctatgccag
     5341 tccttgtacc gatcagcatt gtgttagcag gatttttcca aggaattatc aatacactgt
     5401 tgacgacgat tgctatggaa ataccaggtc tgcaacgaaa cgttgcttcg tccagctata
     5461 gttttgtccg ctttttcggt ggagctttag caccatttat tgcaggtgtg attggtgaac
     5521 atattggtga aagctatgcg ttttattttg caggtgccat tgttcttatc agtgtatgtt
     5581 gtatcattta tcaccgtaat tattttgcta caaatgaaga agaataagaa atcagaggtt
     5641 gtgaaaaagc tgtcctgcat caagtcataa gaa
//
LOCUS       DAZUKC010000284         3008 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.guided.163, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000284 DAZUKC010000000
VERSION     DAZUKC010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.guided.163"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            266..1234
                     /locus_tag="ACG80T_003027"
     CDS             266..1234
                     /locus_tag="ACG80T_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFS4842573.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1227..2258
                     /gene="vanA"
                     /locus_tag="ACG80T_003028"
     CDS             1227..2258
                     /gene="vanA"
                     /locus_tag="ACG80T_003028"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFS4842574.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2264..2872
                     /locus_tag="ACG80T_003029"
     CDS             2264..2872
                     /locus_tag="ACG80T_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFS4842575.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          896 a          571 c          725 g          816 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccaaa ggaggtccta agagatgtat
       61 ataatttttt aggaaaatct caaggttatc tttacttttt cttaggaaat taacaattta
      121 atattaagaa acggctcgtt cttacacggt agacttaata ccgtaagaac gagccgtttt
      181 cgttcttcag agaaagattt gacaagatta ccattggcat ccccgtttta tttggtgcct
      241 ttcacagaaa gggttggtct taattatgaa taacatcggc attactgttt atggatgtga
      301 gcaggatgag gcagatgcat tccatgctct ttcgcctcgc tttggcgtta tggcaacgat
      361 aattaacgcc aacgtgtcgg aatccaacgc caaatccgcg cctttcaatc aatgtatcag
      421 tgtgggacat aaatcagaga tttccgcctc tattcttctt gcgctgaaga gagccggtgt
      481 gaaatatatt tctacccgaa gcatcggctg caatcatata gatacaactg ctgctaagag
      541 aatgggcatc actgtcgaca atgtggcgta ctcgccggat agcgttgccg attatactat
      601 gatgctaatt cttatggcag tacgcaacgt aaaatcgatt gtgcgctctg tggaaaaaca
      661 tgatttcagg ttggacagcg accgtggcaa ggtactcagc gacatgacag ttggtgtggt
      721 gggaacgggc cagataggca aagcggttat tgagcggctg cgaggatttg gatgtaaagt
      781 gttggcttat agtcgcagcc gaagtataga ggtaaactat gtaccgtttg atgagttgct
      841 gcaaaatagc gatatcgtta cgcttcatgt gccgctcaat acggatacgc actatattat
      901 cagccacgaa caaatacaga gaatgaagca aggagcattt cttatcaata ctgggcgcgg
      961 tccacttgta gatacctatg agttggttaa agcattagaa aacgggaaac tgggcggtgc
     1021 cgcattggat gtattggaag gagaggaaga gtttttctac tctgattgca cccaaaaacc
     1081 aattgataat caatttttac ttaaacttca aagaatgcct aacgtgataa tcacaccgca
     1141 tacggcctat tataccgagc aagcgttgcg tgataccgtt gaaaaaacca ttaaaaactg
     1201 tttggatttt gaaaggagac aggagcatga atagaataaa agttgcaata ctgtttgggg
     1261 gttgctcaga ggagcatgac gtatcggtaa aatctgcaat agagatagcc gctaacatta
     1321 ataaagaaaa atacgagccg ttatacattg gaattacgaa atctggtgta tggaaaatgt
     1381 gcgaaaaacc ttgcgcggaa tgggaaaacg acaattgcta ttcagctgta ctctcgccgg
     1441 ataaaaaaat gcacggatta cttgttaaaa agaaccatga atatgaaatc aaccatgttg
     1501 atgtagcatt ttcagctttg catggcaagt caggtgaaga tggatccata caaggtctgt
     1561 ttgaattgtc cggtatccct tttgtaggct gcgatattca aagctcagca atttgtatgg
     1621 acaaatcgtt gacatacatc gttgcgaaaa atgctgggat agctactccc gccttttggg
     1681 ttattaataa agatgatagg ccggtggcag ctacgtttac ctatcctgtt tttgttaagc
     1741 cggcgcgttc aggctcatcc ttcggtgtga aaaaagtcaa tagcgcggac gaattggact
     1801 acgcaattga atcggcaaga caatatgaca gcaaaatctt aattgagcag gctgtttcgg
     1861 gctgtgaggt cggttgtgcg gtattgggaa acagtgccgc gttagttgtt ggcgaggtgg
     1921 accaaatcag gctgcagtac ggaatctttc gtattcatca ggaagtcgag ccggaaaaag
     1981 gctctgaaaa cgcagttata accgttcccg cagacctttc agcagaggag cgaggacgga
     2041 tacaggaaac ggcaaaaaaa atatataaag cgctcggctg tagaggtcta gcccgtgtgg
     2101 atatgttttt acaagataac ggccgcattg tactgaacga agtcaatact ctgcccggtt
     2161 tcacgtcata cagtcgttat ccccgtatga tggccgctgc aggtattgca cttcccgaac
     2221 tgattgaccg cttgatcgta ttagcgttaa aggggtgata agcatggaaa taggatttac
     2281 ttttttagat gaaatagtac acggtgttcg ttgggacgct aaatatgcca cttgggataa
     2341 tttcaccgga aaaccggttg acggttatga agtaaatcgc attgtaggga catacgagtt
     2401 ggctgaatcg cttttgaagg caaaagaact ggctgctacc caagggtacg gattgcttct
     2461 atgggacggt taccgtccta agcgtgctgt aaactgtttt atgcaatggg ctgcacagcc
     2521 ggaaaataac ctgacaaagg aaagttatta tcccaatatt gaccgaactg agatgatttc
     2581 aaaaggatac gtggcttcaa aatcaagcca tagccgcggc agtgccattg atcttacgct
     2641 ttatcgatta gacacgggtg agcttgtacc aatggggagc cgatttgatt ttatggatga
     2701 acgctctcat catgcggcaa atggaatatc atgcaatgaa gcgcaaaatc gcagacgttt
     2761 gcgctccatc atggaaaaca gtgggtttga agcatatagc ctcgaatggt ggcactatgt
     2821 attaagagac gaaccatacc ccaatagcta ttttgatttc cccgttaaat aaacttttaa
     2881 ccgttgcacg gacaaactat ataagctaac tctttcggca ggaaacccga cgtatgtaac
     2941 tggttcttag ggaatttata tatagtagat aacccgtggt gctagttaat tttaatatcc
     3001 taaagaat
//
LOCUS       DAZUKC010000285         1960 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.guided.193, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000285 DAZUKC010000000
VERSION     DAZUKC010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.guided.193"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            193..1104
                     /locus_tag="ACG80T_003030"
     CDS             193..1104
                     /locus_tag="ACG80T_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFS4842576.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1257..1742
                     /gene="vanZ-A"
                     /locus_tag="ACG80T_003031"
     CDS             1257..1742
                     /gene="vanZ-A"
                     /locus_tag="ACG80T_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFS4842577.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
BASE COUNT          696 a          267 c          380 g          617 t
ORIGIN      
        1 acgtgagttg aaattttgac aacctaattt ttctaaggaa attggtgata atagtaaatc
       61 cagtagggcg aaataattga ctgtaattta cggggcaaaa cggcacaatc tcaaacgaga
      121 ttgtgccgtt taaggggaag attctagaaa tatttcatac ttccaactat atagttaagg
      181 aggagactga aaatgaagaa gttgtttttt ttattgttat tgttattctt aatatactta
      241 ggttatgact acgttaatga agcactgttt tctcaggaaa aagtcgaatt tcaaaattat
      301 gatcaaaatc ccaaagaaca tttagaaaat agtgggactt ctgaaaatac ccaagagaaa
      361 acaattacag aagaacaggt ttatcaagga aatctgctat taatcaatag taaatatcct
      421 gttcgccaag aaagtgtgaa gtcagatatc gtgaatttat ctaaacatga cgaattaata
      481 aatggatacg ggttgcttga tagtaatatt tatatgtcaa aagaaatagc acaaaaattt
      541 tcagagatgg tcaatgatgc tgtaaagggt ggcgttagtc attttattat taatagtggc
      601 tatcgagact ttgatgagca aagtgtgctt taccaagaaa tgggggctga gtatgcctta
      661 ccagcaggtt atagtgagca taattcaggt ttatcactag atgtaggatc aagcttgacg
      721 aaaatggaac gagcccctga aggaaagtgg atagaagaaa atgcttggaa atacgggttc
      781 attttacgtt atccagagga caaaacagag ttaacaggaa ttcaatatga accatggcat
      841 attcgctatg ttggtttacc acatagtgcg attatgaaag aaaagaattt cgttctcgag
      901 gaatatatgg attacctaaa agaagaaaaa accatttctg ttagtgtaaa tggggaaaaa
      961 tatgagatct tttattatcc tgttactaaa aataccacca ttcatgtgcc gactaatctt
     1021 cgttatgaga tatcaggaaa caatatagac ggtgtaattg tgacagtgtt tcccggatca
     1081 acacatacta attcaaggag gtaaggatgg cggaatgaaa ccaacgaaat taatgaacag
     1141 cattattgta ctagcacttt tggggtaacg ttagcttttt aatttaaaac ccacgttaac
     1201 taggacattg ctatactaat gatacaactt aaacaaaaga attagaggaa attatattgg
     1261 gaaaaatatt atctagagga ttgctagctt tatatttagt gacactaatc tggttagtgt
     1321 tattcaaatt acaatacaat attttatcag tatttaatta tcatcaaaga agtcttaact
     1381 tgactccatt tactgctact gggaatttca gagagatgat agataatgtt ataatcttta
     1441 ttccatttgg cttgcttttg aatgtcaatt ttaaagaaat cggattttta cctaagtttg
     1501 cttttgtact ggttttaagt cttacttttg aaataattca atttatcttc gctattggag
     1561 cgacagacat aacagatgta attacaaata ctgttggagg ctttcttgga ctgaaattat
     1621 atggtttaag caataagcat atgaatcaaa aaaaattaga cagagttatt atttttgtag
     1681 gtatactttt gctcgtatta ttgctcgttt accgtaccca tttaagaata aattacgtgt
     1741 aagatgtcta aatcaagcaa tctgatcttt catacacata aagatattga atgaattgga
     1801 ttagatggaa aacgggatgt ggggaaactc gcccgtaggt gtgaagtgag gggaaaaccg
     1861 gtgataaagt aaaaagctta cctaacacta tagtaacaaa gaaagcccaa ttatcaattt
     1921 tagtgctgag gaattggtct ctttaataaa tttccttaac
//
LOCUS       DAZUKC010000286         1531 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.guided.205, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000286 DAZUKC010000000
VERSION     DAZUKC010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.guided.205"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            111..194
                     /locus_tag="ACG80T_003032"
     CDS             111..194
                     /locus_tag="ACG80T_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFS4842578.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            319..1056
                     /gene="erm(B)"
                     /locus_tag="ACG80T_003033"
     CDS             319..1056
                     /gene="erm(B)"
                     /locus_tag="ACG80T_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFS4842579.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1226..1486
                     /locus_tag="ACG80T_003034"
                     /pseudo
     CDS             1226..1486
                     /locus_tag="ACG80T_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
BASE COUNT          582 a          242 c          259 g          448 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccaaa ttttgtgtat aataggaatt
       61 gaagttaaat tagatgctaa aaatttgtaa ttaagaagga gggattcgtc atgttggtat
      121 tccaaatgcg taatgtagat aaaacatcta ctgttttgaa acagactaaa aacagtgatt
      181 acgcagataa ataaatacgt tagattaatt cctaccagtg actaatctta tgacttttta
      241 aacagataac taaaattaca aacaaatcgt ttaacttctg tatttattta cagatgtaat
      301 cacttcagga gtgattacat gaacaaaaat ataaaatatt ctcaaaactt tttaacgagt
      361 gaaaaagtac tcaaccaaat aataaaacaa ttgaatttaa aagaaaccga taccgtttac
      421 gaaattggaa caggtaaagg gcatttaacg acgaaactgg ctaaaataag taaacaggta
      481 acgtctattg aattagacag tcatctattc aacttatcgt cagaaaaatt aaaactgaac
      541 actcgtgtca ctttaattca ccaagatatt ctacagtttc aattccctaa caaacagagg
      601 tataaaattg ttgggagtat tccttaccat ttaagcacac aaattattaa aaaagtggtt
      661 tttgaaagcc atgcgtctga catctatctg attgttgaag aaggattcta caagcgtacc
      721 ttggatattc accgaacact agggttgctc ttgcacactc aagtctcgat tcagcaattg
      781 cttaagctgc cagcggaatg ctttcatcct aaaccaaaag taaacagtgt cttaataaaa
      841 cttacccgcc ataccacaga tgttccagat aaatattgga agctatatac gtactttgtt
      901 tcaaaatggg tcaatcgaga atatcgtcaa ctgtttacta aaaatcagtt tcatcaagca
      961 atgaaacacg ccaaagtaaa caatttaagt accgttactt atgagcaagt attgtctatt
     1021 tttaatagtt atctattatt taacgggagg aaataattct atgagtcgct tttttaaatt
     1081 tggaaagtta cacgttacta aagggaatgg agataaatta ttagatatac tactgacagc
     1141 ttccaagaag ctaaagaggt ccctagcgcc tacggggaat ttgtatcgat aaggggtaca
     1201 aattcccact aagcgctcgg gaccccttgt aggaaaatgt cctaagtgtg gcaacaatat
     1261 tgtattaaaa aaatcgtttt atggttgttc aaattatcct gaatgtaagt ttactttagc
     1321 tgaacatttt agaaagaaaa aactaaccaa aacgaatgta aaagaattac tggagggaaa
     1381 agaaaccctg gtaaaaggaa tcaaaaacaa agagaaaaag ccctacaatg ccgttgtaaa
     1441 aattggggaa aagggatata ttgattttat ctctttttca aaataaacat ggggctgacc
     1501 caaaagtagc tcctatatga aaaatagagg t
//
LOCUS       DAZUKC010000287         1268 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.guided.223, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000287 DAZUKC010000000
VERSION     DAZUKC010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.guided.223"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            16..365
                     /locus_tag="ACG80T_003035"
                     /pseudo
     CDS             16..365
                     /locus_tag="ACG80T_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(637..927)
                     /locus_tag="ACG80T_003036"
     CDS             complement(637..927)
                     /locus_tag="ACG80T_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4842580.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1135..>1268)
                     /locus_tag="ACG80T_003037"
     CDS             complement(1135..>1268)
                     /locus_tag="ACG80T_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFS4842581.1"
                     /translation="KLDEATAENSSLKQYIQKLEKAKDEVEDTADKSQQIVEEHLRK"
BASE COUNT          375 a          252 c          189 g          452 t
ORIGIN      
        1 cttttaaata cttaattgtt aaatacctta tttgatgcaa aagaaatcta cgatagtcaa
       61 caaaaaaatt ttcattatat ccagaaaaat ttttcttagt taaggaccta tggattgcag
      121 taatgcagaa ttcgtcgaat aaactgccaa agacatacaa tcatattgct tttaaaattg
      181 acgaacaaga tatagattcg tttgtctcaa aaatccaaat gttaggttta actgtcgaac
      241 caggacgttc tcgtgtgaaa ggcgaggctt cttcaattta cttttacgac tatgataatc
      301 acctcttcga gttacatacc ggtactctcc aggaacgttt aaaatcctac aattccacta
      361 cctgagacta gtaatgacta gtctcttttt atggcctctg atccccccat tgagcgaata
      421 ctaatagggc ttcgaattga tctgttcaat ctttaagcaa tatccctcaa atgagtttgg
      481 aaagtttagt gattcatcac aaatacttaa atacatgtag ttatatatag attgaggttc
      541 ttgtgagcgt atgctcgtgt gggttcaaat cccatcgtct gcatccatct ttccaaaaca
      601 agcggctagg acatttttgt ctcagccgct tgttttctag tttattcttt catcttgtcg
      661 gacctttttt cttgggtata gacttttctc atattctacc acaattttgg gtttccctaa
      721 cacatgatag gatctcacaa caaattgctt tctattatta taataacctg taacgactaa
      781 cttcatccct tcgtctactt cagctaggaa gttcaaacta tgggaagcaa tcaaacaatt
      841 gaccccattt aaactgaaac ggatcaacgg tctggcacta acttgcaaag tacggatctt
      901 atctactgtt ccatattttt tctccataac cttcacctca ataccattat acgaacatac
      961 attccctttg taaagaagaa actctgtttt atctttttga ctttttgtct attacctttc
     1021 ttaaaatcat attggcactt cctgcgaaga tgaaggacat gtactaaaaa aaagaggcag
     1081 cagtatagat tactgttgct tctttactaa tttaggttta cttgttaaga aagattattt
     1141 acgcaaatgt tcttcaacaa tttgctgaga tttatcagct gtatcttcca cttcatcttt
     1201 tgctttttct aacttttgaa tatattgttt caagcttgag ttttcagcag ttgcttcgtc
     1261 taatttct
//
LOCUS       DAZUKC010000288         1078 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.guided.232, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000288 DAZUKC010000000
VERSION     DAZUKC010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.guided.232"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..439
                     /locus_tag="ACG80T_003038"
     CDS             47..439
                     /locus_tag="ACG80T_003038"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4842582.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            440..>1078
                     /gene="aac(6')-Ie"
                     /locus_tag="ACG80T_003039"
     CDS             440..>1078
                     /gene="aac(6')-Ie"
                     /locus_tag="ACG80T_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
                     /protein_id="HFS4842583.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYD"
BASE COUNT          429 a           88 c          195 g          366 t
ORIGIN      
        1 aacattgtat agcattttac acaggagtct ggacttgact gagtttatgg aagaagtttt
       61 aattgatgat aatatggttt ttgatattga taatttaaaa ggatttctta atgataccag
      121 ttcatttggg tttatagcta aagaaaataa taaaattata ggatttgcat attgctatac
      181 acttttaaga cctgatggaa aaacaatgtt ttatttacac tcaataggaa tgttacctaa
      241 ctatcaagac aaaggttatg gttcaaaatt attatctttt attaaggaat attctaaaga
      301 gattggttgt tctgaaatgt ttttaataac tgataaaggt aatcctagag cttgccatgt
      361 atatgaaaaa ttaggtggta aaaatgatta taaagatgaa atagtatatg tatatgatta
      421 tgaaaaaggt gataaataaa tgaatatagt tgaaaatgaa atatgtataa gaactttaat
      481 agatgatgat tttcctttga tgttaaaatg gttaactgat gaaagagtat tagaatttta
      541 tggtggtaga gataaaaaat atacattaga atcattaaaa aaacattata cagagccttg
      601 ggaagatgaa gtttttagag taattattga atataacaat gttcctattg gatatggaca
      661 aatatataaa atgtatgatg agttatatac tgattatcat tatccaaaaa ctgatgagat
      721 agtctatggt atggatcaat ttataggaga gccaaattat tggagtaaag gaattggtac
      781 aagatatatt aaattgattt ttgaattttt gaaaaaagaa agaaatgcta atgcagttat
      841 tttagaccct cataaaaata atccaagagc aataagggca taccaaaaat ctggttttag
      901 aattattgaa gatttgccag aacatgaatt acacgagggc aaaaaagaag attgttattt
      961 aatggaatat agatatgatg ataatgccac aaatgttaag gcaatgaaat atttaattga
     1021 gcattacttt gataatttca aagtagatag tattgaaata atcggtagtg gttatgat
//
LOCUS       DAZUKC010000289          897 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.guided.239, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000289 DAZUKC010000000
VERSION     DAZUKC010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.guided.239"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            284..832
                     /gene="aac(6')"
                     /locus_tag="ACG80T_003040"
     CDS             284..832
                     /gene="aac(6')"
                     /locus_tag="ACG80T_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFS4842584.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          316 a          134 c          185 g          262 t
ORIGIN      
        1 tatggccttt ttacttttac acaattatac ggactttatc gtttttttta aagcaaaaga
       61 taatctatat catgatgaac aaacagagtt ttttcctcca attgatggct ttcctgatct
      121 ttcctatttt gaataaagtg tttctgtata atttttttat tttataagta agataaataa
      181 tgaaagaata gagatactga ttggtagcta aagatttttg agcgagaaca tttcgttttt
      241 tcaaaaggac tgttagaata agcaagaaca agggggaaga cgtatgataa tcagtgaatt
      301 tgaccgtaat aatccagtat tgaaagatca gctttctgat ttactgagac tgacttggcc
      361 ggaagaatat ggagacagct cggcagaaga agtagaagaa atgatgaatc cagaacgaat
      421 cgcggtagca gcggtagacc aagatgagtt agtaggattt attggtgcaa tccctcaata
      481 cggtatcaca ggttgggaat tgcatccatt agttgtagaa agctcccgac gaaagaacca
      541 aataggtact cgattagtca attacttaga aaaagaagta gcttccagag gaggaatcac
      601 gatttattta ggtacggatg atttagacca tggaacaacg ttaagtcaaa cggacctgta
      661 tgagcataca tttgataaag tggcttccat ccagaacctt cgtgaacatc cgtatgaatt
      721 ctatgaaaaa ttaggttata aaatcgtagg tgtcttacca aatgcaaatg gctgggacaa
      781 accggatatt tggatggcaa aaacgattat tcctcgacca aattctcaat aataaaaaga
      841 ggttgtgaca gaagtaatca gcttcaagaa ataagaagga atttacgaaa attactt
//
LOCUS       DAZUKC010000290          860 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5314
            SAMEA115707637-rid22580473.guided.245, whole genome shotgun
            sequence.
ACCESSION   DAZUKC010000290 DAZUKC010000000
VERSION     DAZUKC010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707637
            Sequence Read Archive: ERR13197365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288100.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,981
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 45 of 85
            Pseudo Genes (incomplete)         :: 38 of 85
            Pseudo Genes (internal stop)      :: 15 of 85
            Pseudo Genes (multiple problems)  :: 9 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707637-rid22580473.guided.245"
                     /isolate="VRE5314"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..830
                     /locus_tag="ACG80T_003041"
                     /note="aph(2'')-Ia"
     CDS             <1..830
                     /locus_tag="ACG80T_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HFS4842585.1"
                     /translation="IEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLN
                     TNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFL
                     RQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVF
                     EGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFG
                     EDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRAYKD"
BASE COUNT          370 a           57 c          142 g          291 t
ORIGIN      
        1 gtattgaaat aatcggtagt ggttatgata gtgtggcata tttagttaat aatgaataca
       61 tttttaaaac aaaatttagt actaataaga aaaaaggtta tgcaaaagaa aaagcaatat
      121 ataatttttt aaatacaaat ttagaaacta atgtaaaaat tcctaatatt gaatattcgt
      181 atattagtga tgaattatct atactaggtt ataaagaaat taaaggaact tttttaacac
      241 cagaaattta ttctactatg tcagaagaag aacaaaattt gttaaaacga gatattgcca
      301 gttttttaag acaaatgcac ggtttagatt atacagatat tagtgaatgt actattgata
      361 ataaacaaaa tgtattagaa gagtatatat tgttgcgtga aactatttat aatgatttaa
      421 ctgatataga aaaagattat atagaaagtt ttatggaaag actaaatgca acaacagttt
      481 ttgagggtaa aaagtgttta tgccataatg attttagttg taatcatcta ttgttagatg
      541 gcaataatag attaactgga ataattgatt ttggagattc tggaattata gatgaatatt
      601 gtgattttat atacttactt gaagatagtg aagaagaaat aggaacaaat tttggagaag
      661 atatattaag aatgtatgga aatatagata ttgagaaagc aaaagaatat caagatatag
      721 ttgaagaata ttatcctatt gaaactattg tttatggaat taaaaatatt aaacaggaat
      781 ttatcgaaaa tggtagaaaa gaaatttata aaagggctta taaagattga ttatataata
      841 tatgaaaagc tattataaaa
//
